LOCUS       DAVKLW010000001        78326 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKLW010000001 DAVKLW010000000
VERSION     DAVKLW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 78326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.001"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            126..2018
                     /gene="pepO"
                     /locus_tag="VW324_000001"
     CDS             126..2018
                     /gene="pepO"
                     /locus_tag="VW324_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9725715.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEESWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2141..2770)
                     /locus_tag="VW324_000002"
     CDS             complement(2141..2770)
                     /locus_tag="VW324_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9725716.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(3110..3352)
                     /locus_tag="VW324_000003"
     CDS             complement(3110..3352)
                     /locus_tag="VW324_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725717.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            3763..5985
                     /locus_tag="VW324_000004"
     CDS             3763..5985
                     /locus_tag="VW324_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9725718.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALIYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            6015..6458
                     /locus_tag="VW324_000005"
     CDS             6015..6458
                     /locus_tag="VW324_000005"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9725719.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTDIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6497..6912)
                     /locus_tag="VW324_000006"
                     /pseudo
     CDS             complement(6497..6912)
                     /locus_tag="VW324_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7270..7632
                     /locus_tag="VW324_000007"
     CDS             7270..7632
                     /locus_tag="VW324_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725720.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEEDVLILLSELQNFFLDVFQAKRIHRA
                     FCVRLCLKAQFLLSNNDS"
     gene            7813..8112
                     /locus_tag="VW324_000008"
     CDS             7813..8112
                     /locus_tag="VW324_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725721.1"
                     /translation="MNRDVLEFLRTETAEKISLYISEANRLEGDVTLLAPSSQDLEDI
                     KNAMLSNSNLGLKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(8207..8644)
                     /locus_tag="VW324_000009"
     CDS             complement(8207..8644)
                     /locus_tag="VW324_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725722.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(8746..9411)
                     /locus_tag="VW324_000010"
     CDS             complement(8746..9411)
                     /locus_tag="VW324_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9725723.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVEISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIEGNSN
                     KEY"
     gene            complement(9599..10249)
                     /locus_tag="VW324_000011"
     CDS             complement(9599..10249)
                     /locus_tag="VW324_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9725724.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            10361..11203
                     /locus_tag="VW324_000012"
     CDS             10361..11203
                     /locus_tag="VW324_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9725725.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMNFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            11279..11716
                     /locus_tag="VW324_000013"
     CDS             11279..11716
                     /locus_tag="VW324_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9725726.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            11785..12087
                     /locus_tag="VW324_000014"
     CDS             11785..12087
                     /locus_tag="VW324_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725727.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLDFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(12178..13251)
                     /locus_tag="VW324_000015"
     CDS             complement(12178..13251)
                     /locus_tag="VW324_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9725728.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDAELQLYFDLFFGTILDAGISSINDLTSLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            13407..14084
                     /locus_tag="VW324_000016"
     CDS             13407..14084
                     /locus_tag="VW324_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9725729.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            14077..15051
                     /locus_tag="VW324_000017"
     CDS             14077..15051
                     /locus_tag="VW324_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9725730.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(15100..17043)
                     /gene="thrS"
                     /locus_tag="VW324_000018"
     CDS             complement(15100..17043)
                     /gene="thrS"
                     /locus_tag="VW324_000018"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9725731.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LEHYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(17101..17277)
                     /locus_tag="VW324_000019"
     CDS             complement(17101..17277)
                     /locus_tag="VW324_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9725732.1"
                     /translation="MTKQGRYYDETSYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(17284..17424)
                     /locus_tag="VW324_000020"
     CDS             complement(17284..17424)
                     /locus_tag="VW324_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9725733.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            18294..18923
                     /locus_tag="VW324_000021"
     CDS             18294..18923
                     /locus_tag="VW324_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9725734.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSSAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKEHISLLEK"
     gene            19064..19333
                     /gene="rpsO"
                     /locus_tag="VW324_000022"
     CDS             19064..19333
                     /gene="rpsO"
                     /locus_tag="VW324_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9725735.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            20149..20763
                     /locus_tag="VW324_000023"
     CDS             20149..20763
                     /locus_tag="VW324_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9725736.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(21055..21804)
                     /locus_tag="VW324_000024"
     CDS             complement(21055..21804)
                     /locus_tag="VW324_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9725737.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            21937..24273
                     /locus_tag="VW324_000025"
     CDS             21937..24273
                     /locus_tag="VW324_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9725738.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            24374..25051
                     /locus_tag="VW324_000026"
     CDS             24374..25051
                     /locus_tag="VW324_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9725739.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            25038..25835
                     /locus_tag="VW324_000027"
     CDS             25038..25835
                     /locus_tag="VW324_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9725740.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            25852..26955
                     /locus_tag="VW324_000028"
     CDS             25852..26955
                     /locus_tag="VW324_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9725741.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQAPANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            26956..27036
                     /locus_tag="VW324_000029"
                     /pseudo
     CDS             26956..27036
                     /locus_tag="VW324_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            27256..28275
                     /gene="galE"
                     /locus_tag="VW324_000030"
     CDS             27256..28275
                     /gene="galE"
                     /locus_tag="VW324_000030"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9725742.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            28277..29242
                     /locus_tag="VW324_000031"
     CDS             28277..29242
                     /locus_tag="VW324_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9725743.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFSAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(29293..29499)
                     /locus_tag="VW324_000032"
     CDS             complement(29293..29499)
                     /locus_tag="VW324_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9725744.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            29551..30027
                     /locus_tag="VW324_000033"
     CDS             29551..30027
                     /locus_tag="VW324_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9725745.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            30036..30707
                     /locus_tag="VW324_000034"
     CDS             30036..30707
                     /locus_tag="VW324_000034"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9725746.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            30837..31175
                     /locus_tag="VW324_000035"
     CDS             30837..31175
                     /locus_tag="VW324_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9725747.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            31178..31807
                     /locus_tag="VW324_000036"
     CDS             31178..31807
                     /locus_tag="VW324_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9725748.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            32047..33198
                     /locus_tag="VW324_000037"
     CDS             32047..33198
                     /locus_tag="VW324_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9725749.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            33511..34260
                     /gene="truA"
                     /locus_tag="VW324_000038"
     CDS             33511..34260
                     /gene="truA"
                     /locus_tag="VW324_000038"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9725750.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            34250..35023
                     /locus_tag="VW324_000039"
     CDS             34250..35023
                     /locus_tag="VW324_000039"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9725751.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            35001..35462
                     /locus_tag="VW324_000040"
     CDS             35001..35462
                     /locus_tag="VW324_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9725752.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            35689..37030
                     /locus_tag="VW324_000041"
                     /pseudo
     CDS             35689..37030
                     /locus_tag="VW324_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(37158..37361)
                     /locus_tag="VW324_000042"
     CDS             complement(37158..37361)
                     /locus_tag="VW324_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9725753.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(37354..38436)
                     /locus_tag="VW324_000043"
     CDS             complement(37354..38436)
                     /locus_tag="VW324_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9725754.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(38562..39344)
                     /locus_tag="VW324_000044"
     CDS             complement(38562..39344)
                     /locus_tag="VW324_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9725755.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(39344..40687)
                     /locus_tag="VW324_000045"
     CDS             complement(39344..40687)
                     /locus_tag="VW324_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9725756.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(40786..42102)
                     /locus_tag="VW324_000046"
     CDS             complement(40786..42102)
                     /locus_tag="VW324_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9725757.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            42295..43521
                     /locus_tag="VW324_000047"
     CDS             42295..43521
                     /locus_tag="VW324_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9725758.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            43720..45294
                     /locus_tag="VW324_000048"
     CDS             43720..45294
                     /locus_tag="VW324_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9725759.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            45559..46347
                     /gene="codY"
                     /locus_tag="VW324_000049"
     CDS             45559..46347
                     /gene="codY"
                     /locus_tag="VW324_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9725760.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            46347..46922
                     /locus_tag="VW324_000050"
     CDS             46347..46922
                     /locus_tag="VW324_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9725761.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            47004..47089
                     /locus_tag="VW324_000051"
     tRNA            47004..47089
                     /locus_tag="VW324_000051"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47038..47040,aa:Leu,seq:aag)
     gene            47372..48502
                     /gene="ugpC"
                     /locus_tag="VW324_000052"
     CDS             47372..48502
                     /gene="ugpC"
                     /locus_tag="VW324_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9725762.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(48625..48795)
                     /locus_tag="VW324_000053"
     CDS             complement(48625..48795)
                     /locus_tag="VW324_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725763.1"
                     /translation="MEIKEQTRKLAVSYSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDV"
     gene            49013..49771
                     /locus_tag="VW324_000054"
     CDS             49013..49771
                     /locus_tag="VW324_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9725764.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            49858..50370
                     /locus_tag="VW324_000055"
     CDS             49858..50370
                     /locus_tag="VW324_000055"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9725765.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            50552..51496
                     /locus_tag="VW324_000056"
     CDS             50552..51496
                     /locus_tag="VW324_000056"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9725766.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            51505..52182
                     /locus_tag="VW324_000057"
     CDS             51505..52182
                     /locus_tag="VW324_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9725767.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            52280..53038
                     /locus_tag="VW324_000058"
     CDS             52280..53038
                     /locus_tag="VW324_000058"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9725768.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            53076..54581
                     /gene="lytC"
                     /locus_tag="VW324_000059"
     CDS             53076..54581
                     /gene="lytC"
                     /locus_tag="VW324_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9725769.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWYGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            55077..55595
                     /locus_tag="VW324_000060"
     CDS             55077..55595
                     /locus_tag="VW324_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9725770.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            55725..56231
                     /locus_tag="VW324_000061"
     CDS             55725..56231
                     /locus_tag="VW324_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9725771.1"
                     /translation="MTKKIVAIWAQDKEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPGELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            56231..56401
                     /locus_tag="VW324_000062"
     CDS             56231..56401
                     /locus_tag="VW324_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725772.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            56433..57665
                     /gene="clpX"
                     /locus_tag="VW324_000063"
     CDS             56433..57665
                     /gene="clpX"
                     /locus_tag="VW324_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9725773.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            57674..58261
                     /locus_tag="VW324_000064"
     CDS             57674..58261
                     /locus_tag="VW324_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9725774.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            58272..58652
                     /locus_tag="VW324_000065"
     CDS             58272..58652
                     /locus_tag="VW324_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9725775.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            58704..59594
                     /gene="rapZ"
                     /locus_tag="VW324_000066"
     CDS             58704..59594
                     /gene="rapZ"
                     /locus_tag="VW324_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9725776.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKCLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            59591..60568
                     /locus_tag="VW324_000067"
     CDS             59591..60568
                     /locus_tag="VW324_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9725777.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            60565..61476
                     /gene="whiA"
                     /locus_tag="VW324_000068"
     CDS             60565..61476
                     /gene="whiA"
                     /locus_tag="VW324_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9725778.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHYQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(61510..62475)
                     /locus_tag="VW324_000069"
     CDS             complement(61510..62475)
                     /locus_tag="VW324_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9725779.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            62611..63468
                     /locus_tag="VW324_000070"
     CDS             62611..63468
                     /locus_tag="VW324_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9725780.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            63455..64234
                     /locus_tag="VW324_000071"
     CDS             63455..64234
                     /locus_tag="VW324_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9725781.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            64258..65610
                     /locus_tag="VW324_000072"
     CDS             64258..65610
                     /locus_tag="VW324_000072"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9725782.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            65684..66058
                     /locus_tag="VW324_000073"
     CDS             65684..66058
                     /locus_tag="VW324_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9725783.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            66060..66908
                     /locus_tag="VW324_000074"
     CDS             66060..66908
                     /locus_tag="VW324_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9725784.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNDPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            67174..67941
                     /locus_tag="VW324_000075"
     CDS             67174..67941
                     /locus_tag="VW324_000075"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9725785.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            67953..69137
                     /locus_tag="VW324_000076"
     CDS             67953..69137
                     /locus_tag="VW324_000076"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9725786.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            69134..71005
                     /locus_tag="VW324_000077"
     CDS             69134..71005
                     /locus_tag="VW324_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9725787.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDATLLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPIAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(71148..72332)
                     /gene="mntE"
                     /locus_tag="VW324_000078"
     CDS             complement(71148..72332)
                     /gene="mntE"
                     /locus_tag="VW324_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9725788.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDILRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            72636..75332
                     /locus_tag="VW324_000079"
     CDS             72636..75332
                     /locus_tag="VW324_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9725789.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALITFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            75345..76136
                     /gene="yghU"
                     /locus_tag="VW324_000080"
     CDS             75345..76136
                     /gene="yghU"
                     /locus_tag="VW324_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9725790.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            76252..76662
                     /locus_tag="VW324_000081"
     CDS             76252..76662
                     /locus_tag="VW324_000081"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9725791.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            76674..78269
                     /locus_tag="VW324_000082"
     CDS             76674..78269
                     /locus_tag="VW324_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725792.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        22968 a        14613 c        17848 g        22897 t
ORIGIN      
        1 ttatgcgttt tatcatggaa agatttttta taatagctaa aaaataatag ttaaatggtt
       61 attaattgca ttccctagtg atttttgtta agataaatgc aaatacaaat gaaagcgaga
      121 acaagatgac acgttatcaa gatgattttt atgatgctat caatggagaa tggcaacaga
      181 cagctgaaat cccagcagat aagtctcaaa caggaggttt tgttgattta gaccaggaaa
      241 ttgaagacct gatgttggcg acaacagaca agtggttagc aggtgaagaa gtgcctgagg
      301 atgctatctt ggaaaacttt gtcaaatacc accgcctagt tcgtgatttt gacaagagag
      361 aagctgacgg tatcacacct gtcttaccac tccttaaaga attccaagaa ttggaaactt
      421 ttgcggattt tacagctaaa ctagcagagt ttgagcttgc aggaaaacca aacttccttc
      481 cttttggtgt atcgccagac tttatggatg ctagaatcaa tgttctatgg gctagcgctc
      541 caagcacaat cttgccagat acgacctact atgcagaaga acatcctcag cgcgaagaac
      601 tcttgactct ttggaaagaa agcagcgcaa atctcctcaa ggcttatgat ttctctgatg
      661 aagaaattga agacttgcta gaaaaaagac ttgaattgga ccgccgagtt gcggcagtgg
      721 tgctctctaa tgaagaaagt tcagaatatg ctaaactcta tcatccatat tcttacgaag
      781 atttcaagaa attcgcgcct gccctacctt tggatgactt cttcaaagca gttattgggc
      841 aattaccaga caaggttatt gtagacgagg aacgtttctg gcaagcagca gagcaattct
      901 acagtgagga atcctggtct ctccttaaag caaccttgat tttgagtgtt gtcaatcttt
      961 caaccagcta tttaacagag gatatccgtg ttttgtctgg tgcctacagc cgtgcccttt
     1021 ctggagttcc agaggcaaaa gataaggtca aagcagctta tcatctagca caagaacctt
     1081 tcaagcaagc cctggggctt tggtacgccc gtgagaagtt ctctccagaa gccaaggcgg
     1141 atgtggagaa aaaagtggca accatgattg atgtctataa ggagcgtctg cttaagaatg
     1201 actggctcac tccagaaacc tgtaaacagg ctatcgtgaa gctcaatgtg atcaaacctt
     1261 atattggcta tccagaagaa ttgcctgcac gttacaagga taaggtagtg aatgaaactg
     1321 ccagtctttt tgagaatgct ctagcctttg cgcgtgtgga aatcaagcac agttggagta
     1381 agtggaacca gcctgtagac tataaggaat ggggcatgcc tgctcatatg gtcaatgcct
     1441 actacaatcc tcagaagaac ctgattgtct ttccagcggc cattttacag gcgcctttct
     1501 atgacttgca tcagtcatct tctgctaact acggtggtat tggggcagtg attgcccatg
     1561 aaatttccca cgcctttgat actaacgggg cttcctttga cgaaaatggt agcctcaagg
     1621 attggtggac agagagcgac tatgctgcct tcaaggagaa aacacaaaaa gtcattgacc
     1681 aatttgatgg acaggattct tatggagcaa ccattaacgg taaattgact gtatcagaaa
     1741 acgtggctga cttgggagga atcgcagcag cgcttgaagc agctaagaga gaagcagact
     1801 tctcagcaga agagttcttc tacaacttcg gtcgcatctg gcgcatgaaa ggtcgaccag
     1861 aatttatgaa acttttggct agcgtcgatg tgcacgcacc agccaaactc cgtgtcaatg
     1921 tgcaagtacc aaacttcgac gatttcttta caacctatga tgtcaaagaa ggagacggaa
     1981 tgtggcgttc accagaggag cgcgtgatta tttggtaata caaaaaatct agtcatcaga
     2041 aaaaaggcat ccaatcatgt gtgaaaacct tgataggatg ccttttgtaa tagaaagatt
     2101 tgctggatag tttacttaaa tttcgattgt catcatcttg ctagaaaaag ggattgaagg
     2161 ccttttcgtg agcgatagta gtagctggac catgtcctgg atagacatcg tagtttggta
     2221 gggtgaagag ttgggtctgg atactatgaa ggagttgctc catgctacca gtcggaaggt
     2281 cggtccgtcc gatagtttcg cggaatagag catctcccgt caagactagg tgagcatcag
     2341 gaaagactag ggaaacacca ccgatagagt gccctggggt cggtagaacc ttaaaacgaa
     2401 attcctctag ttggtattct tcgtgaaaga caaaggtgtg ttctgcaggt tttgtgacca
     2461 catctgccat atcatcgtgg cgagggagac cggagagatt atcgacagga gtgtagagcc
     2521 agctggcttc gctctctgcg atatagacag gaggattgcc aaacgtctcg cgaaccaagt
     2581 ccagactcat gatatggtca taatgggcgt gggtcaagag aatagcacag atcggtttgt
     2641 tgatcttctc gattgtctga cgaatggctt cccaatgact accaggatcg acgacgatga
     2701 ggtgcttttc gccttctaga taataggtat tttcataggc aacaggattc acggttttat
     2761 ggattttcat actagctcca atctcaaaga atgtactaat actcttcgaa aatctcttca
     2821 aaccgcgtca actttatctg caacctcaaa gcagtgcttt gaacagcctg cggctagctt
     2881 tctagtttgc tctttgattt tcattgagta taaattaagt atagcacagt tagggagaat
     2941 aggtaaggat ttaaaatgaa cttaaaaacc agcataacta gatgaagaca ggttggttat
     3001 caatttatca atattggaaa ggtgtcaatt gcccttcata ggttgcataa actccgtcat
     3061 tagcttgttt gatagacgtg atgtttagag tgctaccgta gttggcttct catttgtcgc
     3121 tatattcact ataagttatc ctactgggca ggttctggtc gttagcatag tattgaacga
     3181 gttttttata gctttgctgg ctgaatagga acaggcagca aactcctaac attaggagag
     3241 caaagataga aatagcagtt ttttcatagg tattctcctt ataaactaaa ggatagaaga
     3301 aagtatcttg aggattttta tactcttcaa aaatctcttc aaaccgcgtc aacgtcgcct
     3361 tgccgtatat atgtgactga ctttgtcagt cttatctaca acctcaaagc agtgctttga
     3421 acagcttgcg gctagtttcc tagtttgctc ttggattttc attgagtatt aattccaaaa
     3481 tccagtcaag gtaccagtgt atgtgatacg agttccaccg tttatctttc gtatcttcaa
     3541 tggaattggg gaagtcagaa agtctttggg ttacaatatg gtttccgttc ttctatctct
     3601 tcttctatca cctaagttta aaatctaact tcgtagtata ttatgggcca ataattcaga
     3661 attatcaagc aattttgaat tatttttttc ttattctgtt ccaatctagt tttcatattc
     3721 ttaaaaaagt gataaaatgg taggaagaat tggagagtag agatgccgaa agaagtgaat
     3781 ttaacaggcg aagaagttgt cgctttaacc aaagaatatt taacggaaga ggatgttcat
     3841 tttgtccata aggccttgat ctatgctgtt gaatgccaca gtggtcaata tcgcaaatca
     3901 ggcgagcctt atatcattca ccctatccaa gtggcaggta ttttagctaa gctaaagctg
     3961 gatgctgtaa cagtagcttg tggattcttg catgatgtgg tggaagatac agatgcgacc
     4021 ttggacgatt tggaaagaga gtttggtcct gatgtgcggg tgattgttga cggagttacc
     4081 aagcttggca aggtcgagta caaatcgatc gaggagcaat tagcggaaaa tcatcgcaag
     4141 atgctcatgg ccatgtctga ggacatccgc gttattttgg tcaaactgtc tgaccgcttg
     4201 cacaatatgc ggaccctgaa acatcttcga aaagacaagc aggagcgtat ttccaaagaa
     4261 accatggaaa tctatgcccc acttgcccat cgtttgggga tttccagtgt caaatgggaa
     4321 ttagaagact tgtctttccg ttatctcaat ccaacggagt tttacaagat tacccatatg
     4381 atgaaggaaa agcgcaggga gcgtgaggcc ttggtggatg aggtagtcac aaaattagag
     4441 gagtatacga cagaacgtca cttgaaaggg aagatttatg gtcgtcccaa gcatatttac
     4501 tcaattttcc gcaaaatgca ggacaagaga aaacggtttg aggaaatcta tgatctgatt
     4561 gctattcgtt gtattttaga tacccaaagt gatgtttatg ccatgcttgg ttacgtgcat
     4621 gaattttgga aaccgatgcc aggtcgcttc aaagactata ttgccaaccg caaggccaat
     4681 ggttatcagt ctatccatac gactgtttat ggaccaaaag ggccgattga attccagatt
     4741 cgaaccaagg aaatgcacga ggtggctgag tacggggttg cggctcactg ggcttataag
     4801 aaaggtataa aggggcaagt taacagcaag gaatcagcta ttggaatgaa ctggatcaag
     4861 gagatgatgg agctccaaga ccaggctgat gatgctaagg aatttgtgga ctctgttaag
     4921 gaaaactatc tggctgagga gatttacgtt tttaccccag atggagctgt ccgttccctt
     4981 cccaaagatt caggaccgat tgattttgcc tacgaaatcc ataccaaggt cggtgaaaaa
     5041 gcaactggtg ccaaggtcaa tggccgcatg gttccactga caaccaagtt aaagacaggg
     5101 gatcaggttg aaattatcgc caacccgaac tcctttggac ctagccgtga ctggctcaat
     5161 atggtcaaga ctagcaaggc gcgcaataag attcgccagt tctttaaaaa ccaagataag
     5221 gaattgtctg tcaacaaggg tcgtgagatg ctgatggctc agttccaaga aaatggctat
     5281 gtggcaaata aatttatgga caagcgccac atggatcaag ttctgcaaaa gaccagttac
     5341 aagacagaag actccctctt tgcggccatt ggttttgggg aaatcggtgc gattaccgtc
     5401 tttaaccgtc tgactgaaaa ggaacgccgt gaggaagagc gtgccaaggc caaggctgag
     5461 gcagaggagc ttgtcaaagg tggcgaggtc aaggttgaaa ataaagaaac tctcaaggtc
     5521 aagcatgagg ggggagtggt tattgaaggt gcttctggtc tcctagtgcg gattgctaag
     5581 tgttgtaacc ccgtgcctgg tgacgatatt gttggctaca ttaccaaggg tcgtggtgtg
     5641 gctattcacc gtgtggactg tatgaacctg cgtgcccaag aaaactacga gcaacgtctc
     5701 cttgatgtgg aatgggaaga ccagtactct agctcaaata aggagtatat ggcccatatc
     5761 gatatctacg gtctcaaccg tacaggactg ttgaacgatg tactgcaagt tctttcaaat
     5821 acaaccaaga atatttcaac ggtcaatgcc caaccaacca aggatatgaa gtttgctaat
     5881 atccatgtgt ccttcggtat tgccaacctc tctacactga ccacggttgt cgataaaatt
     5941 aagagtgtgc cagaagttta ctctgtcaaa cggaccaacg gctagttgtc ctagctctta
     6001 ctagaaaggc tattatgaaa atcattatcc aacgggttaa aaaagcccaa gtgagtatag
     6061 aaggccagat tcagggaaaa atcaatcagg gacttttatt gctagttggt gttggaccag
     6121 aggaccaaga ggaagatttg gactatgctg tgagaaaact ggtcaatatg cggatttttt
     6181 cagacgcaga aggcaagatg aacctgtctg tcaaagatat tgaaggagaa atcctctcta
     6241 tttctcagtt taccctcttt gcggatacta agagaggcaa tcgtccagcc tttacagggg
     6301 cagctaaacc tgatatggca tcagacttct atgatgcttt caatcaaaaa ttagcgcaag
     6361 aagtgcccgt tcagacagat atctttggag cagatatgca ggttgagctg gttaataacg
     6421 gacccgttac cattatccta gatactaaaa agagataaaa aagaccaagc ccagtcggct
     6481 tggtctttct catctatcat aaaatactcc aaaaagaaat cggttcttga tatgcttggg
     6541 ggaagtctct tttcaggctt tggcagatgc gataggaagg gatgagatgt cctaggatga
     6601 ggagagttcc ctgaaggaaa agtggaatat cacttaaaga caccaaggag agaatcatca
     6661 ggctatccca taggagaatt tgtaaagcaa aacgccagaa tctcggtcta atctgggaat
     6721 agagctgact aaagtggtca caaagttggt ttgaaagata ggtcaatagc acaggatgaa
     6781 agaaaatgag ccaaccgcta taaatcaaaa tccagtccca agtaagcccc cctttttaaa
     6841 tgtcaaaaat gccagcatga ttccatcaat gacaaggagt tgaatggttt tgatgaagat
     6901 gatttttttc atgctaaaac ctccttttcc ttagagacac tcccctacag gtattttaga
     6961 atgtatattg cttagatatt ccttatttta ttagcttttt aaggtattaa agtagttata
     7021 taagtttatg aagggtatcc tatcctgcac ggctaatttt gagttcgaaa ctgaggagtt
     7081 tataactcaa aatttcgatt aaatcagcat ttaacgatag tatgtttgtc ataaaatgat
     7141 aaatttttag attgtggaaa ttttttttaa cttaattctt gatttttttc taaatgtgat
     7201 atacttaatt tacgggataa aaaaaccacc ttagaaaggt gtgataatgt gcaaaaagag
     7261 aatgttagaa tgcgtttagc gtctgaatcc agacagatat tagataagct taaagatgat
     7321 gtttacaata aattaggcta tgaagtaagc tattccagta ttgttagtca agctgttaga
     7381 gaatacgttc ctaaaaaaga acgtatagat tggataaaat taaaagagac agctataccc
     7441 ttttcatcgt taaaacagag caataattgg gagtaccaga catcacttat gttagaggaa
     7501 gatgtattaa tcttactgtc tgaactacaa aattttttct tagatgtctt ccaagcgaaa
     7561 cgtatacata gggcattttg tgtacgccta tgcttgaaag cacaattttt attaagtaat
     7621 aatgattctt aatactatca atattaatgc ggagtgtaat gctccgctaa aataaaacct
     7681 attacgggat aaaaacacta ctttatttag gatggaaaga ggaacgaatg aagatgaaag
     7741 ctagagattc accattgaat tgattttgtt tttagaaaaa atcaataatt agaaaaaata
     7801 cgaggtaaaa aaatgaatag agatgtatta gagtttctga gaacagagac cgctgaaaaa
     7861 attagtcttt atataagcga ggctaataga ttggagggcg atgtaactct actcgcacct
     7921 agttctcaag atttggaaga tattaaaaat gctatgcttt ccaattctaa tctcggatta
     7981 aaagttgcga gactggatgt gatgaagaaa atagcatatg cttctacaag aaatcattat
     8041 ttaacagggg ctacgatttt tggggatatt tcgaaaggaa catacaattg tgatcctaaa
     8101 agttatgtct aatataaggg aggttaatcc ctccccttgt ttttgaaaat cgaaaggaaa
     8161 agtataaaaa tgaaagttaa tgaatatttc tggagaacga gctgtcttat actaaaaaag
     8221 aagaaatcga taaaatagta tacgtgttag agatgtgttt gtcgtaaaag tctggatagt
     8281 tttctcctag gtagtcagct gtcatataat aaaaagtaga atggcaagca gtgataattg
     8341 gcataagaga tgaaaaacgc tgagaactaa tgaaggctaa gagaacaaaa aatagaaaat
     8401 caatggtgag aacccctaaa tagtagaagc gaatcttttt attgatttga ggataaatct
     8461 cagcgaattg atgtttgagt cggacgacca agagaaatag caggagtcct tgagcaagga
     8521 agaaaattaa accagagagc aagagccact caaaagatgt ctcgagtcta gtgataaaaa
     8581 atgcaaatag tagcaaatca ataactgcaa ttgctataaa atggattcta tagagttttt
     8641 tcatgacaag accttcctct tttatctaac ttcattctac tccaaaagaa tgggagttac
     8701 aactaaaatg ataaaaatag cagaagggag attctcttaa gttggctagt attctttatt
     8761 tgagtttcct tctatgtatc taacttcttc atcattccag acaaataaag ctccgattgc
     8821 attgaggata taaaagatgt atttaccgat attggcgaag tttccttgaa taccagcttt
     8881 tgtcagctga acgaaattgt aaatcaacca aaagccccac tgagttgtta gtttcaatgc
     8941 attcaaagca ttggcaatga gggacagtgc aaaggcaata gttgttacgt aggcaaggag
     9001 attcatcttg ccctcatatc cgatatagtt ggtcacaaag gcaaagagga aggcgatgat
     9061 ggaaatgatg atggccgcca attttacctg tttttggctc atttggttgg gtctgccttc
     9121 ttgcgaagct ttccatttct ttatagcaaa ggtataaatg aggaaggtga cgggataggt
     9181 aatgatggcc gccttatttc caaggatata atcaatagca ccggacaaaa tggtattaac
     9241 aataccaaag taatttcccc atttgcttaa tttccccgtg aaacgagtgg acaacatgga
     9301 aatctcaacg ttggttacgg aaatcaatcc aaagggtaca agagctgtcc atggtcccca
     9361 gtctacaaat ttatcgaggc gtgagttgag gtaaccagat gcaatcgcaa tccaacgacc
     9421 aaagcaaccc cgaagaggtc aaactattta gatgtagcaa aaatttttag tgattttttc
     9481 ataggttaaa ctacctttct ttttttcaaa tattctccca ccaaatgaaa gtaaaataaa
     9541 atgatagaaa taaaaccctg aaaataaaac tttcagggtt ggtaggggac ttgagaaatt
     9601 agttgatttt ctcgacatag agttgttttg caatgtccat attcacttgt aaatcctcgt
     9661 cgttactgag gatagtgaag gtattgctga agccatcaaa ctgcttgact tggagctggt
     9721 caccgatgtg aagtgagtgc ttgtccagat aatggagaat gtcaaaatta tcgtgcaccc
     9781 gagtcaggcg gtaggcgcca gcttccttga tatcagctag tgggaggtta ttgatttcaa
     9841 cgaggagttc tcccttggca ggaatagttc ctccgtgggg gcaggtttta gggaaaccta
     9901 gcagtttatc tagtctttcc acgaacaggt cagagacagt gtgttccaag acctcagctt
     9961 cctcgtgaat ctggtcactt gtatagtcta aatgatgaac tagaaaaact tcaatcaagc
    10021 ggtgcttacg atagagctca gagactagtt tgagaccgag gtcagtcagt agatagccac
    10081 attccttgtc ctttaggatg aggttttcac ttttcattcg tttgatcatt tcagttacgg
    10141 cagggggaga gacttgcatg cgagccgcaa tttccttgtt ggtaatctta tgcaggtcta
    10201 tgccaatttc ataaatacat tttagatagt cttctttgtt tggggtcatt cgtttcctct
    10261 tgtctaatat ctttatctag tatatcaaaa aaagctagat ttctctagct gtgacttttt
    10321 atgttatact tattgcaaga gaaaaaacga ggagatggat atgacgaaaa tagctcttct
    10381 ttcagatatt catggaaata ccaccgcctt ggaggctgtt ttggcagatg ctcggcagct
    10441 aggagtggat gaatactggc ttttgggaga tattctcatg ccagggacag gacgtagaag
    10501 gattttggac ttgttggatc aactaccgat tacggctaga gttttgggaa actgggaaga
    10561 cagtctttgg catggtgtcc gtaaggaatt ggatagtact cgccccagtc aacgctatct
    10621 cttgcgccag tgccagtatg ttttagagga aatttcccta gaagaaattg aagtgctcca
    10681 caatcaacct cttcaaattc atcgtcagtt tggggatttg acggtgggaa ttagtcatca
    10741 tctgcctgat aagaactggg ggcgagagtt gattcatact ggcaaacaag aggattttga
    10801 ccgcttggtg actcatcctc cctgtgatat tgctgtttat ggacatattc accagcagtt
    10861 gcttcgttac gggactggtg ggcaattgat tgtcaatccg ggttcgattg gccaaccttt
    10921 ctttttggac gctcagctac ggaaggactt gcgggcccag tatatgattt tggagtttga
    10981 tgacaagggc ctggtagata tgaacttccg acgggtagac tacgatgtag cagctgaatt
    11041 gcagctggct aaagacctga gacttcccta ttttgaggtt tactatgaaa gtctggtcaa
    11101 tgggatccac catactcatc atcaggaatt tttgagagaa ttggctcaga aagagggttg
    11161 cgatcgggag ttagacgact ggttgaaaag tggtaacgat tgacattaca actaaaaagg
    11221 gtataataat agaaaaagaa gagtagaggc agggtagcct cgtaaaaagg agaagaggat
    11281 gcaaattcca agtagattta ccattgcgac tcatatgctg ataatcattg ccctcgaggg
    11341 gaaggaaagc aaggtgacca gtgattttct ggctgctagt gtcggggtca atcctgtcat
    11401 tatcagaaag atcttgtccc agttgaagaa ggcagagctg atttcagtag cgcgtggaac
    11461 gggcggaaca gagattgtca aggaccttaa ggatattagt cttttagatg tttatcaggc
    11521 ggtcgaatgt cttggtaaga caggtcaact cttcagtttc catgacaatc cgaatccaaa
    11581 ttgccctgta ggagctcata ttcatgatgt tttggatcaa aaattggaga gaattcagtt
    11641 gactatggag gcagaacttg gtcagaccag tctagaaaaa gtcgtggccg atgcagagag
    11701 tcagatgaag gattaagagg gataaatgcc ctcttttttt ctttggataa ttatgataaa
    11761 atgtagctat taaaggagta agaaatgtat ctcgttatag gaattgtatt agcctttata
    11821 gtgtcatttt ggaaggataa tagaagtttg tggaatccag tattattttt attatccctc
    11881 atttcaagtt atttttatct atcctacctt ttttataaaa atggatacga gaatgttcag
    11941 ttagattttt atgtatttgc ctttgtctta cttccttttt tgctttttct aagtggcatc
    12001 tttttaatct ataatggggt cattttgttg aagcgagagg gacgttccaa gccccattat
    12061 ctctcaatgc tctttgattt ttattgacta ttagattcta tttaaaaaga cttagaatgc
    12121 acatgaaagt atgtattcta ggtcttttgt tttgtataca tttaattttc aagagtatta
    12181 tctatttttc catgcctggt aacgagtatc aatcaataac tgggtaaggc gacccaaggt
    12241 ttctctttct tctggagtga gggattgagc ttggacaaag agatgatgaa tgtattcaag
    12301 cgcctttaag gaagtcaagt cattgataga gctaatacca gcatcaagaa tagtgccaaa
    12361 gaagaggtcg aagtagagct gaagttctgc gtcagggact gtggccaccc attctttaaa
    12421 ggttgtgtct acttgctggc tatcactgtt ggtcttatcc agttggacga agtgcttgtc
    12481 ctcaatctgc caactaaagg tatcgtgctg ggcgatgcca cccagggcag tactctgaac
    12541 gatgatttgg tgagcaggaa tttccagcat cataccgata atggaacctt gtggaatgaa
    12601 tatcttgctt ctatccatta tcctttgata acccgcagtc tgtgtcaatt cttgatggag
    12661 accaggtgca tcaaatgtat aaactgctgt gatctgattt tgcaaacttt gctcaatttg
    12721 gctagcagca tagatagcga gatttcctcc cttggaatgc ccagccagaa taaccttttg
    12781 cttaggatga tgggcaaaaa agttctttaa atagcgaagg gcgtgctttt gagcaggaat
    12841 ttccttcata taggtcaggt ggaaatcttc cttccagcca atgatactgt catctgtccc
    12901 acgaaagaca atcagatagg tatcgaggct gacacgataa gtcatagccg caaattgctt
    12961 ttgcagttca gggtcgatgt cgttgataaa atgggagagt ttgcaatttt tgaagcgctt
    13021 gtgttgagcc aattcatcta ataattgaag gcgatttttg ctagtaagca tggtgggctc
    13081 tcttggaacc tgaggtgcta gatctaaaag acgttgagga agtgtggaga ccagattatc
    13141 aaaggagagg taggtgattt ctattaaggt tagaatgtct aactcattca agggaaggtc
    13201 gtaataagaa tcatatgcga catctttcag atagtcaaaa atattggcca taagagctcc
    13261 tttcttttta tttatcttag caaatttccc tcaaattagc tagtagcatt gcctgtttgt
    13321 actggctaaa aacaggctat ttcaaattca gtttcagacc atctggcatg gaaaaatctg
    13381 ttataataat agaaaaggag aagcgcatgc acaagatttt attaatagaa gatgatcagg
    13441 tcattcgtca acaggtcggg aaaatgctct ctgaatgggg atttgaagtg gtcctggtag
    13501 aagactttat ggaagttttg agtctatttg ttcagtcgga acctcatctg gtcctcatgg
    13561 atattggttt gcccttgttt aatggttatc actggtgtca ggaaatccgc aagatttcca
    13621 aggtacctat catgtttctt tcttcgagag accaggctat ggatattgtc atggcaatca
    13681 atatgggggc ggatgacttt gtgaccaagc cttttgacca gcaggttctt ttagctaagg
    13741 ttcagggctt gttgcgtcgt tcctatgagt ttgggcgtga tgagagtttg ctggaatatg
    13801 ctggtgttat cctcaatacc aaatccatgg atttacatta tcaagggcaa gtcttgaatt
    13861 tgaccaagaa tgaattccag attttacgcg tgttatttga gcatgcaggc aacatcgtag
    13921 cacgtgacga cctgatgcgg gaactttgga acagtgactt tttcattgat gataataccc
    13981 tctctgtcaa tgtggctcgt ttgcgtaaaa agttggagga gcagggattg gtaggattta
    14041 tcgagaccaa gaaaggaata gggtacggat tgaagcatgc ttgattggaa acaatttttt
    14101 ctagcctatc tgcgctcccg tagtcgtctt tttatctatc tgctttcttt ggcatttctt
    14161 gtcttactct ttcagttttt atttgccagt ctaggaattt acttcctcta ctttttcttc
    14221 ttgtgttgct ttgtaaccat cttatttttc acttgggaca tattggtgga aacgcaggtc
    14281 tatcgccagg aacttctcta tggagagagg gaagccaagt ctcctttgga aatagcttta
    14341 gcagaaaaat tagaagcgcg tgagatggaa ctctatcagc agaggtcaaa agcagaaaga
    14401 aaactgacgg atttgctgga ttactatacc ttgtgggtcc atcagataaa gacccccatt
    14461 gcagccagtc aactcttagt tgcagaagtg gtcgaccgcc aactgaagca gcagctagaa
    14521 caggaaattt tcaaaatcga ctcctatacc aacctagttt tacagtacct gcgtttagaa
    14581 agtttccatg atgatttgct cttaaagcag gttcaaattg aggacttggt caaggaaata
    14641 attcgtaaat atgctctttt ctttattcaa aaaggcttaa atgtcaatct acatgacctt
    14701 gataaagaaa tcgtgacgga taaaaagtgg ctgctagtgg ttattgagca aatcatctca
    14761 aacagtctca agtacaccaa ggaaggtggt ctggagattt atatggatga ccaagagctt
    14821 tgtatcaaag atacgggaat cgggataaaa aacagtgatg tcctccgagt atttgaacgt
    14881 ggcttttcag gatacaatgg ccgtttgacc cagcagtcct ctggacttgg cctttatcta
    14941 tctaagaaaa tttctgaaga actggggcac cagattcgta tcgagtctga ggtcggaaaa
    15001 ggaacgacag tgcggattca gtttgctcaa gtgaacttag tccttgagta aaaatagatg
    15061 agaaaaagcc tccagattgg aggcttttat gctagactct tatttctcaa cgcgtgattt
    15121 gttggcgata tcagctagga tagcttgaac aaaattatca actgagacag tttgtgtttc
    15181 tttttggccg tagcgacgaa cgttgactgt ttcgtcttcc atttctttgt ctccaacaat
    15241 taattggtaa ggaatcttgc tggtttgtga agcacggatc ttgaactgca ttttttcatt
    15301 gcgctcatct acgtctgcac ggacaccgcg gtcacggagt ttcttggcca cttcccaagc
    15361 gtagtccacg tgtttttcgt tagatactgg gatgagggtt acttggtgtg gtgccagcca
    15421 tgttgggaag gcccccttgt agttctcaat caagatagct gtgaagcgtt ccatagttga
    15481 gataacccca cggtggatca tgactggacg gtgatcttcg ccatcagctc cgatgtattt
    15541 gaggtcgaag cgttctggca acaagaaatc aagttggata gtagaaaggg tttcttcttt
    15601 tccaagggca gttttaatct ggatatccaa ttttggtccg tagaaggctg cttcaccttc
    15661 ggcttcaaag tagtccacgc ccatttcatc aagagctgca cgaagcatgg tttgggcatt
    15721 ttcccacatc tcatcgttat caaagtactt atgagtatct tgagggtcac gaagagagag
    15781 gcggaagcgg tagtcagtca agttgaagtc ttcataaaca tcgataatca actgaagggc
    15841 acgttggaat tcttcttgga tttgttctgg agtaacgaat aggtgaccgt cgttgagtga
    15901 catttcacgt acacgttgaa ggccagtgag ggcaccagat ttttcgtaac ggtgcatcat
    15961 accgatttca gcgatacgga ttggcaattc acggtaagag tgaacatggt gtttgaaaac
    16021 ttggatgtgg tgcggacagt tcattggacg aaggacaaat tcttccccgt cacccatgtc
    16081 catggttggg aacatgtctt cttggtaatg atcccagtga ccagaagtct tgtaaagctc
    16141 aacagaagca agtggtggag tgtagacgtg ttggtagcca gaagccaact ctttgtttac
    16201 gatgtagtgt tccaattcac gacggatagt cgcaccattt ggcaaccaga atggcaaacc
    16261 ttgtcccact tcttgtgaaa tcataaagag gtcaagctct ttaccaagtt tacggtggtc
    16321 acgttcctta gcttcttcac gcatttgaag gtagtttttc aagtctttct tgtcaaacca
    16381 agctgtaccg tagatacgtt gcatcatagc gttgtcgctg tttccacgcc agtacgcacc
    16441 agctacatgg agaaggtgga agatttggat acgacctgtt gatggaacgt gaggtccacg
    16501 gcagaggtct acatattcac cctgacgata gatagtcaaa ccgccttcgt cttctgagtg
    16561 ttcttcaatc aattccaact tgtaagggtc atttttgaag atttcacgtg cctcgtcttt
    16621 agtcacttct tcacgaatag atgggaagtt ttctttgacg attttttgca tttcttcttc
    16681 gatacgagga aggtcttcgt tagagatttg accagctgtg ttgtcagtat cgtagtagaa
    16741 accatcttcg atggctggac caactcccaa gtgaatgtct gggaaaagac gacgagctgc
    16801 ttgggcgaac aagtgagctg ctgagtgacg caagattgga agggcatctt cgtgatcagg
    16861 tgtcacaatt tcgatgcttc catcttcagt gatagcgcga gtagtgtcga tgagtttgcc
    16921 gttgaattta ccagccaagg cttttttagc tagggaattg ctgatagatt gggcaatttc
    16981 aaaagttgtt acgccagatt cgaattcacg aacagcgcca tctgggaaag taatgttaat
    17041 catgatgttc tcctttttta ttatttcata gtttctaagc attgattttt tatcagcaga
    17101 ctacttccga gtcctaacgt tcaattgctt ttctttatca gagaggaagt tttccatttc
    17161 cgtcagaatg tctatatcca aacgttgaga aagttctaat agccaccaga tattttctga
    17221 aagtttttgt tccagtgtgt aggatgtttc atcatagtag cgtccttgct ttgtcatcac
    17281 cagtcattgg aaatttccaa tatcattaga taaagccaag aggtcttctt ctaccgtcca
    17341 cttgaaatca tgatgcttaa cttccagttc gtgataagct tgtcggattg cccaactgcg
    17401 ctccactaat tcttgcaact ccataattag tatcctaata aaaaaatgcg acatcctcga
    17461 aaggacgtcg caacgtggtt ccaccttcat ttatgtacct caaaagagag ggacacctct
    17521 gatcggctct aacgtggcca ccgttttatg ttttcataaa aaactcaaga gtagtatcag
    17581 tttagacttc ctatgcgttt ccagcaacca cgcactctct agtagaaagg gactaagtga
    17641 cttgtctcta tggattatta tagcatgatt gtgagatttt tcaaggattt tgtgaaagtt
    17701 ttttgttttt cattttttgt agaatatgct tgatgctaat accagaatcc atgcccacca
    17761 gagcaactgt ttcatagatt agcaagtaaa ttagtataaa ctcaccaaag gttatgatag
    17821 taaagtaaaa gagcagtccg ttaccaacta accatcaaag tgagaaacgg aaacggtagc
    17881 tatagagtaa cgatacaatc aagattacca taggtgtaaa gaagagaaga ttgtttatat
    17941 agagtccctt gagtgtaagg gggtcagaaa caactagcaa gaacaactca taaagtggcc
    18001 aataaaagaa taatatgacc agatagtagg ggagatggga atagaatcta cgcatgataa
    18061 tcacctcgtt ttaaaaaaac agacaactta gatctcttgc tatgattatt atagcatgtt
    18121 taaaacaaga aagtaaattt gttgacaaag aagatttttc ttttcactag aatcatagtt
    18181 agttttcttg gtagaaatat tttgataata attaagcaag actattgata ttgattatta
    18241 agagtgtcac aattgttgta actaaaatga ttacaacaat agaggtataa aaaatgaaac
    18301 tagcagttat tgcagcaaat ggacaagcgg ggaaagcaat tgttgaagaa gcagtcaaac
    18361 gaggacatga agtaacggct attgttcgtt ctgaaaacaa aagtcaagct gagtctatta
    18421 ttaaaaaaga tttatttgag ttgacaaaag atgatcttac tggatttgat gctgttattt
    18481 cagcgtttgg tgcttataca ccagatacgc taccgctcca cagcaaatca atcgaactat
    18541 ttaatcagct tcttgctgga acacagacac gttttctagt tgtaggggga gcaggaagtc
    18601 tttatataga tgaaacaaaa acgacacgac ttttagatac gtctgatttt cctgaagaat
    18661 ttaagtcact agcaaaagat caagcggatg aattggatct tcttcgtaca aaaaataatc
    18721 tcaactggac ttttgtcagt tctgctgtag attttattcc cgatggtgaa aaaacaggaa
    18781 actacattct agcaggagaa atttttacta ctaatgaaaa aggaataagc cagattagtt
    18841 atgcagatta tgctattgga ttggtagatg aacttgaaaa aggtcatcat ataaaagaac
    18901 atatctcttt acttgaaaaa taataactat gagagtatta atgtttgaca gaaaatttaa
    18961 atcttggtaa aatagggatt gtcgtaaaga caaataactt cttcttggtt acaggcatgc
    19021 caacctgtca ctcggatgaa gccaaataaa aaggagaaac atcatggcaa tctcaaaaga
    19081 gaaaaaaaat gaaatcattg cacaatatgc acgtcacgaa ggtgatacag gttcagtaga
    19141 ggttcaagtt gctgtcctta cttgggaaat caaccacctt aacgaacaca tcaaacaaca
    19201 caaaaaagac cacgctactt accgtggatt gatgaaaaaa atcggtcgcc gtcgtaactt
    19261 gcttgcatac ttgcgtaaaa acgacgttaa ccgttaccgt gagttgatca actctctagg
    19321 acttcgtcgc taattcaaga tacaaatgct gtcaaaagca caaagcaaaa ataggaaaat
    19381 tgacgaagaa acttcagttt ctaggagatt ttatcttttt tgccaagtgc ttaggccgtg
    19441 ttcaattgag catatcttga taatgaagct actctaaatt gggtagcttt tttgcgttgt
    19501 caccttacct cgatatactc aagtataatc tttggttacg gttcctagca ctgtaaggta
    19561 aaataaacca gcttttctcc cttcggggag attttttttg tttcactgaa agtttcgtac
    19621 gatcttcgcc tcgtcgccta gtctataaac gatttatcta gcaagaatca tgatgctaag
    19681 ggcgtcaaaa atccgtatga aaatagggaa atgacacagt gttcgatgag cacaaggagt
    19741 ttcatctttt tcactaggat tttagcccgg gctcaaatca gctctctgac ttcagagggc
    19801 ttttttattg aggttttatc ggtaacaatt ttggagacta caaaaacctc aaacggtatc
    19861 agctaattac accataaagg tgcgcagtta ctcggctttt caagccgagt agctgtatgc
    19921 aagcccccta gaagagccca ctctttacaa agtaaagtat agtatgttat actttacatg
    19981 gaagtagtca ctgaattcca gttagaaatt actttgtaac tacgttttga ggaggagtaa
    20041 aatgctttcc tacgttcgac attacccact agcgatagct aaattaatgt gtctgtgctc
    20101 tcctaaaatc tgctgattta ttactgacta atacaggagg ttttttttat gggacagaca
    20161 atcatatctg ctattggtgt ttatatttcc accagtatcg attatttaat tattttaatt
    20221 attttatttg cacagctatc acagaataaa cagaaatggc atatttatgc ggggcaatat
    20281 ctaggcacag gcttacttgt aggggcgagt ttagttgctg cttatgtcgt taatttcgtg
    20341 cctgaagaat ggatggttgg attgcttggt ttaatcccta tctatttagg gattcgcttt
    20401 gcaattgttg gagaagatgc ggaagaagaa gaggaagaaa ttattgaaag attagaacaa
    20461 agcaaggcaa atcaactgtt ttggacagtt acattgctga caattgcgtc tggcggagat
    20521 aatttaggta tctatatacc ttattttgct tcgttagatt ggtcacagac cctcgtggcc
    20581 ttgcttgtgt ttgtaatcgg cataattatc ttttgcgaga ttagtagggt gttatcctct
    20641 attccgttaa tattcgagac aattgaaaaa tacgagcgaa tcattgtgcc cttagtattc
    20701 attctacttg gactatacat catgtatgaa aatggcacga tagagacttt tctgatcgtg
    20761 tagatttttt tgtttcacta ggattttagc ccgagctcaa atcagctctc tgattttcag
    20821 agggcttttt cattgagtat aaatggacaa tcttaaagtt actctattgt agagtggctt
    20881 ttgatttgat taggagattc ttataaatat agtaaaatga aataagaata ggacaaatcg
    20941 atcaggacag tcaaatcgat ttctaaaaat gttttagaag tagaggtgta ctattctagt
    21001 tccaatctac tatataaatg aaaaccttag ctgtttggcc aaggttcatt tcttctatgc
    21061 aagttcttct cttttcttat ctgggttcca gatgaagctt gcgataaagc taaagatgat
    21121 ggttaggata gcgaggataa tagcaaggat gagggcaggg atgcggataa accaccagag
    21181 tggatttggt tttttatcat tgtgccattg tttcgtttct tcgtccagac gttggaattc
    21241 tgtttgaata cgattggcga ctgtttcaat gccttcatca ttcattttct tgatatctga
    21301 gatatcgatt ggatttccaa agttcatatc gacacgttca cggctaatca agcccttcaa
    21361 agtcatggga ccggtgtagg taaccggcat gatacggacc ttggccattt tggcaatcag
    21421 tgctgcgccc cccttgacat cgtttgagtg gcggctacca cttggaaaca tgatgagaga
    21481 gcggtcactt tttttgagaa cgttgatagg atatttgatg gctgaggcgc tgggattttc
    21541 acggtcgatg ggaaaggcgc cacacatacg aatccaccaa ccaaagatac ggttggtaaa
    21601 gagttctttt tttgccataa agatgaactg ttttggcttg gtcgcaaagg ccatataaac
    21661 aggatcccac caggtacggt gaggcgcaac taaaatataa ttttcatctt gattaggaat
    21721 tttatcagta ttatgatagt gagcattgcc attgatggac cataagagca atacaactaa
    21781 tccacgcaaa taagtataaa acatgcgatc tccttcgatt gttttcttgt tattattata
    21841 ccttatcaaa ggagggctgg caaacttttc ccttgactag atacatattt aggatgaaat
    21901 tagaattctg ttaaaaaaaa tgatataata gaatttatgg ataaaaataa gattatggga
    21961 ttaacccaaa gagaagtcaa ggaaagacag gctgagggtt tggtcaatga ctttaccgca
    22021 tcagccagta ccagcacttg gcaaatcgtt aaacgaaatg tctttaccct ttttaacgct
    22081 ttgaacttag ccattgcttt ggctcttgcc tttgtgcagg cttggagcaa tctggtcttc
    22141 tttgctgtta tctgctttaa cgctttttct gggattgtga ccgagctacg agccaaacac
    22201 atggtggaca agctcaatct catgaccaag gaaaaggtca aaaccatccg tgatggtcag
    22261 gaagttgctc ttaatcctga agaattagtg ctaggagatg tcattcgttt gtctgcagga
    22321 gagcagattc ctagtgatgc cttggttttg gaaggctttg cggaagtcaa tgaagccatg
    22381 ttaacgggag aaagtgattt ggtgcaaaag gaagttgacg gcttactttt gtcaggaagt
    22441 ttcctagcca gtgggtcagt tttatctcaa gttcaccatg tcggtgcaga caactatgct
    22501 gccaaactca tgcttgaggc taagaccgtt aaacccatca actcccgtat catgaaatcg
    22561 ctggacaagt tggctggttt tactgggaag attatcattc cctttggtct ggctctcttg
    22621 ctggaagcct tgcttttaaa aggcctgcct ctcaagtcat ctgttgtaaa ctcgtcgaca
    22681 gctcttttgg gaatgttgcc taagggaatt gcccttttga ccattacttc gctcttgact
    22741 gcagttatca agctaggttt gaaaaaggtc ttggtgcagg agatgtactc tgttgagacc
    22801 ttggcgcgcg tggatatgct ctgtctggac aagacgggta ccatcaccca aggaaagatg
    22861 caggtggagg ctgttcttcc gttgacggaa acgtatggtg aagaggctat tgccagcatc
    22921 ttgactagct acatggccca tagtgaggat aagaatccaa ctgcccaagc cattcgccag
    22981 cgttttgtgg gagatgttgc ttatcctatg atttccaatc ttcccttctc gagcgaccgc
    23041 aagtgggggg ctatggagtt agaaggcttg gggacagttt tcttaggggc acctgagatg
    23101 ttgctggatt ctgaagtccc agaagccagg gaggccttgg agagaggatc acgtgtcttg
    23161 gtcttagctc tcagtcagga gaaattagac catcacaaac cacagaaacc atctgatatt
    23221 caggctctag ccttgctgga aatcttggac cccattcgag agggagcagc agagacgctg
    23281 gactatctcc gttctcagga ggtaggactc aagattatct ctggtgacaa tccagttacg
    23341 gtgtccagca ttgcccagaa ggctggtttt gcggactatc acaactatgt agattgctca
    23401 aaaatcaccg atgaggaatt gatggccatg gcggaggaga cagctatttt cggacgtgtt
    23461 tcccctcatc aaaagaaact catcatccaa acgttgaaaa aagcgggtca tacaacggct
    23521 atgacagggg acggggttaa tgatatcttg gcccttcgtg aggcggattg ttctatcgtg
    23581 atggcggagg gggatccagc aacccgtcag attgccaatc tggttctctt aaactcagac
    23641 tttaatgatg ttcctgagat tctcttcgag ggtcgtcgcg tggtcaataa cattgcccac
    23701 atcgccccga ttttcttgat aaagaccatc tattccttcc tgttagcagt catctgtatt
    23761 gccagtgctt tactaggtcg gtcagagtgg attttgattt tccccttcat tccgatccag
    23821 attaccatga ttgaccagtt tgtggaaggt ttcccaccat tcgttttgac ttttgagcga
    23881 aatatcaaac ctgtcgagcc aaacttcctc agaagatcca tgcttcgtgc cctaccaagt
    23941 gccctcatgg tcgtgtttag cgttcttttt gtgaaaatct ttggaacgag tcaaggttgg
    24001 tctgagttag aaatctcaac tctcctctat tatctcttgg ggtcaattgg tttcttatcc
    24061 gtatttagag cctgcatgcc attcactcta tggcgagtcc tcttgattgt ctggtcagta
    24121 ggaggcttcc tagccacagc tctcttccca agaatccaaa aactgcttga aatttcaacc
    24181 ttgacagaac aaacgttgcc tgtttatggt gtcatgatgt tggtctttac cgtgattttc
    24241 atcctgacta gtcgctatca agctagaaaa taaagaaaga ctgcaatctg tggattgcag
    24301 tttttttagg tacaagattg ccagccgaaa tgtggtataa taagaggtaa tagagtttgg
    24361 aaagtgagag aaaatgattt caaagagatt agaattggta gcttcctttg tgtcacaggg
    24421 ggctatttta ctagatgtgg gaagtgacca tgcttatctg cctatcgagt tggttgagag
    24481 aggccaaatc aaaagcgcta ttgcaggtga ggtggtggaa ggtccctatc agtctgcggt
    24541 taaaaatgtt gaggctcacg gcctaaagga gaaaatccaa gtccgtttag ccaatggctt
    24601 ggcagctttt gaagagactg accaagtgtc tgtcattacc attgctggca tgggtggtcg
    24661 tttgattgct aggattttag aagaaggttt ggggaagtta gctaatgtag agcgtttgat
    24721 cctccagccc aataatcgtg aagacgactt gcgtatctgg ctacaggatc atggattcca
    24781 gattgtagca gaaagcatct tagaagaagc tggaaagttt tatgagattt tggtggtgga
    24841 agcaggacaa atgaagctat cagccagtga tgttcgcttt ggtcccttct tgtccaaaga
    24901 agtcagtcca gtatttgtcc aaaaatggca aaaagaagct gagaagctag agttcgccct
    24961 cggacaaatc ccagaaaaaa atctggaaga acgtcaagtt ctagtagata agattcaagc
    25021 tatcaaggag gtgccccatg ttagcaagtg aagtgatcca agcttatgaa gccttttgcc
    25081 ctcaggaatt ttctatggag ggagacagcc gtggtctgca aattggcact ttagacaagg
    25141 gtatccaaag ggtcatggtt gccctcgata ttcgtgaaga gacagtggca gaggccattg
    25201 aaaagggtgt ggacttgatt atcgtcaagc acgcgcctat ctttcgtcct atcaaggact
    25261 tgcttgccag ccgtccgcaa aatcagattt acatcgacct gattaagcat gatatcgcag
    25321 tttatgtcag ccataccaat attgatatcg ttgaaaatgg gctcaatgac tggttttgtc
    25381 agatgctagg aatcgaggag acgacttatc tgcaggaaac aggtccagaa cgtggaattg
    25441 gacgtattgg gaatattcag cctcagacat tttgggaatt ggcccaacag gtcaagcaag
    25501 tctttgacct agatagcctt cgaatggtgc attatcaaga gaatgatttg cagaagccta
    25561 tttcaagagt agcaatttgt ggtggaagcg ggcagtcttt ctataaggat gctttggcaa
    25621 agggggcaga tgtctatatc actggcgaca tctactacca tactgctcag gatatgctgt
    25681 ctgatggctt gttagcattg gatccaggtc actatatcga agtgcttttt gtggaaaaaa
    25741 tcgcagcact ccttactcaa tggaaagaga aaaaaggctg ggaactagag attttaccta
    25801 gtcaagcatc gaccaatcct ttccgtcata tctagttaga aagtgaagac aatgaaaaaa
    25861 gttgccatta ttggagcagg aattgtggga gcaacagctg cctactacct ctcgagagaa
    25921 agtgacctag aggtgaccgt ttttgaccat gagcaaggtc aagccaccaa ggccgcagca
    25981 ggaattatca gtccttggtt ttccaaacgc cgtaataaag cctggtataa gatggcgcgc
    26041 ttgggggctg atttttatgt ggatttatta gctgatttag agaaatcagg acaagaaatc
    26101 gacttttacc agcgttcggg agtctttctc ttgaaaaagg atgaatccaa gttggaagaa
    26161 ctctatcaac tggctctcca gcgcagagaa gaatctccct tgataggcca gttagccatt
    26221 ctggaccaag ccccagctaa tgaattattc cctggcttgc agggattcga caacttgctc
    26281 tatgcttctg gtggagcgag agtagatggt cacctcttag taactcgttt gctggaagcc
    26341 agtcaggtca agttggtcaa agaaaaagtg actctgacac tcttatcatc aggctaccag
    26401 attggcgaag aggtgtttga tcaggttatt ttggcaacgg gagcttggtt gggggacatg
    26461 ttagagcctt taggttatga agtggatgtc cgtcctcaaa aaggacaact acgagattat
    26521 cagcttgtcc aagacatgga agattaccct gttgtcatgc cagaagggga gtgggatttg
    26581 attccctttg caggtgggaa attatccttg ggcgctaccc atgaaaatga catgggattt
    26641 gatttgacgg tagatgaacc cttgctccaa caaatggagg aggcggcctt gcctcattac
    26701 ccagttttag ctagagtgac ttcaagggct gagcgtgtgg gaatccgtgc ctataccagt
    26761 gatttctctc ccttctttgg gcaggtgcca gaattggcag gtgtctatgc agccagtgga
    26821 ctaggttcat caggcctcac aactggtcct atcattggtt accatctagc ccaactgatc
    26881 caagacaagg agttgacctt ggaccctcta aattacccaa ttgaaaacta tgtcaaacga
    26941 gtaaaaagcg aataatactc aatgaaaatc aaagaacaaa ctaggaaact agccgcgggt
    27001 tactcaaagc actgctttga ggttgtggat agaactgacg aagtcagctc aaaacactgt
    27061 tttgaggttg tggatagaac tgacgaagtc agtaaccata cccatggcaa ggcgacgttg
    27121 acgtggtttg aattggattt tcgaagagta taagcatttt actgaaattt tatcaggtag
    27181 tttaggatgg caaatgacat tccctgtcaa aaatggtaaa ataagaaaaa atgatctgag
    27241 aatcgaggaa aaaagatgca agaaaagatt ttggtaactg gtggtgccgg ttttattgga
    27301 acccacactg ttattgaatt gatccaagca ggccatcaag ttgttgtggt ggataatctt
    27361 gtcaatagca atcgtaagag tttagaagtt gttgaaagaa tcacaggagt tgagattcct
    27421 ttctatgagg cagatattcg tgacacagat accctcagag atattttcaa gcaggaagaa
    27481 ccgactggtg tcattcactt tgctggtttg aaggctgttg gcgaatcaac acgtatccct
    27541 cttgcctact atgacaacaa tatcgctgga actgtcagcc ttttgaaagc catggaagaa
    27601 aacaactgta aaaacatcat cttcagttct tctgcgacag tttacgggga tccgcacaca
    27661 gtgcccatct tggaagattt cccactttca gtgacaaacc catacggtcg taccaagctc
    27721 atgctagagg aaattttgac cgatatttac aaagcagact cagaatggaa tgttgtcttg
    27781 cttcgttact ttaacccaat cggagcccat gagagtggtg atttgggaga aaatccaaac
    27841 ggtattccaa acaatctctt gccatatgtg actcaagtag ccgttggaaa attagagcaa
    27901 gtgcaagtgt ttggagacga ttacgatacg gaagatggaa caggtgttcg tgactatatc
    27961 cacgttgtcg atttggctaa gggtcacgtt gcagctttga aaaaaatcca aaaaggttca
    28021 ggactaaacg tttataacct tggaactggt aaaggttact cagttcttga aattatccaa
    28081 aacatggaaa aagcggtggg acgtcctatt ccttaccgca tcgtagaacg tcgcccaggt
    28141 gatatcgctg cctgctactc agacccagca aaagctaaag cagaactcgg ttgggaagca
    28201 gaactcgaca tcacccaaat gtgtgaagac gcatggcgtt ggcaaagcaa gcatccaaat
    28261 ggatttgaag actaagatga tgatttcaat catcgtccct tgtttaaacg aagaggaagt
    28321 acttcctctt ttttatcagg ctctggaagc tttacttcca gatttggaaa cagaaatcga
    28381 gtatgtcttt gtcgatgatg gatcaagtga tgggaccttg gaactcttaa aggcctatcg
    28441 ggagcaaaat ccggcagtcc attatatttc tttctctcga aattttggca aagaagcagc
    28501 cctttatgca ggcttgcaat atgcgacagg agatttggtg gtggtgatgg atgcagacct
    28561 ccaagatcct cctagtatgt tgtttgagat gaaaaatgta ctagacaaaa atgtagactt
    28621 ggactgcgtt gggacacgga gaactagtcg ggagggagaa cccttctttc gcagtttctc
    28681 tgctgttctc ttttatcgcc tcatgcaaaa aatcagccca gtagctctgc cgtcgggtgt
    28741 ccgtgatttt cgtatgatga gaaggtctgt agtcgatgcc attttaagct tgaccgagtc
    28801 caatcgtttt tcaaaggggc tcttcgcctg ggtgggtttc aaaacctact atttggacta
    28861 tccaaatgtc gaaaggcagg ctggcaagac aagttggagt tttaggcaac tctttttcta
    28921 ctccattgaa gggattatta atttttcaga ttttcccttg attatagcct ttgtagctgg
    28981 tctcctatct tgttttcttt ctctgctgat gacctttttt gttgtggttc ggaccctcat
    29041 tttgggcaat ccgacatctg gttggacctc tctgatggct gttattctct ttcttggagg
    29101 cattcaactc ttgaccattg ggattctcgg caagtatatc agtaagattt atttagagac
    29161 taaaaaaaga ccactttatc ttatcaaaga aaaaagtgac cttcctgatt ttacagaaaa
    29221 aaataaagtg aaaagactat aattttacat ggaaatgtgc taaactagag ggagtggatt
    29281 atcgccattg atttaggctt cctggtttcc aatcagggcg cgagttgggc aatttttaac
    29341 agcctctaag acatcctgac taggagaaat ttctttttcc agttggtcag ggtcatcgta
    29401 aaaacgcacg attccattat cgtggtaatc aaataaatca gaataagttt ggcaaagccc
    29461 acaggcgata catcgttcag gtataagtgt gattttcatg ttcatattta tattgtaata
    29521 agaaaagtaa aaaaaaacaa ggagtaaggt atggcaaaag aaccgtggca agaagatatc
    29581 tatgatcaag aagaatcaag agcagagcgt cggcatcgaa accacggagg ggcggatagg
    29641 atggctaatc gtattttgac gatcctagct agtattttct ttgtaattgt ggtggtgatg
    29701 gtcatcgttc tcatctatct atcatcgggg gggagtaatc gcacagcagc cttaaaagac
    29761 tttcatgatt ctgatgcaag tgtagtacaa atctcatctt caagcagttc tcagcctgag
    29821 cagagttcag agccagaatc tacttctagt agttcagaag aagctgctaa tcctgaagga
    29881 acgattaaag ttctcgcagg agaaggggaa gcagctattg ccgctcgtgc aggaatctcc
    29941 attgctcagt tagaggcctt gaatcctggg cacatggcta caggatcttg gtttgctaat
    30001 ccaggtgatg ttataaaaat aaaataggag ccgatatgaa aacaattcaa attgctattg
    30061 atggtcctgc ttccagcggt aagagtacgg tcgcaaagat tattgctaag gattttggat
    30121 tcacctacct tgatacagga gctatgtatc gtgcagcgac ctatatggct cttaagaacc
    30181 aattaggagt tgaagaagtc gaagcccttc tagccttgtt ggaccagcat ccaatcagct
    30241 ttggacgttc agaaactgga gaccagcttg tttttgtagg agatgtggat attacccatc
    30301 ctatccgtga aaatgaagtg accaatcatg tttctgctat tgcagcaatt cctgaagtgc
    30361 gtgagaaact ggtttctctc caacaagaaa ttgcccagca aggcgggatt gttatggatg
    30421 gtcgcgatat tggaactgtt gtattgccgc aagcagaatt gaaaattttc ctagtagctt
    30481 ctgttgatga gagagcagag cgtcgttaca aggaaaatat tgccaaggga attgaaacag
    30541 accttgaaac cctaaaaaag gaaattgctg cgcgtgacta caaggatagt catcgtgaga
    30601 cttctcctct caaacaagca gaggatgctg tctaccttga tacaactggt ttgaacattc
    30661 aagaagtagt tgaaaaaatc aaagcagaag ctgaaaaaag aatgtaaatg tagaaaagcc
    30721 gatagattga tatcggtttt ttctaaattt gtcaaaatgc tgattttagg gtataatagt
    30781 tgctgttcga gaaattttga ttttcaaaga atagtgaatg atgataagga gaaatcatga
    30841 acaatttacc aaattgtcca aaatgtaact cagagtatgt ctacgaagac ggtgccctac
    30901 tggtttgccc agagtgtgct catgagtgga atcctgctga agttgcagaa gtagaagaag
    30961 gtcttgtcgc tatcgatgcc aacggaaata aattgactga tggtgataca gtaactctca
    31021 tcaaggactt gaaagtaaaa ggtgcgccaa aagatttgaa acaagggacg cgcgtgaaaa
    31081 atatccgcat cgtagaaggc gatcacaata tcgactgtaa gattgatggt tttggtgcca
    31141 tgaaacttaa atcagagttt gtgaggaaga tttaagcatg tcaaaacact ataaacttgt
    31201 attttatagc cgtatcttct tgtttctagc ggcttttacg ggagtttatc ttgaaattac
    31261 taggcatggt ggttttggga tgcttctcta ttacacggtt ctgtccaacc tcttggtaac
    31321 tatttttacc ctttatcttc taaaggttat gagccgtgta ggtgaaaatt ggcaaaggcc
    31381 aagtctcttg cgcttaaaag gtggggtcac catgagtatc atgattacct gtgtgattta
    31441 ccatttcctc ttggcgccca ttgcgactaa tttctatacc ctagaaaatt tcctttgcca
    31501 ctatatcgtt cccatctggt ttttagcgga taccctcttt tttgacaaac agggtcaata
    31561 caagatttgg gatccagcag tgtggaccat tttacccttt ctgtatatga tgtttgctct
    31621 ttttaatggc ttggttctaa aactcaatat tccaaatgcc aaggataatc ctttccctta
    31681 cttctttttg aatgtgaaca agggttggaa tgttgtgttt aagtggtgtc tgattatctt
    31741 tgttgcctat atggtagcag gatttatttt ctactttatc aagcaaatca agagaaagtc
    31801 atcctaatac tcttcgaaaa tctcttcaaa ccacgttagt ttccatctgc aacctcaaaa
    31861 cactgttttg aacaacctgc ggctagcttt ctagtttgct ctttgatttt cattgagtat
    31921 gagatacaag ggattcattt tcgaatttaa gaaggagtct acaaaccaat gaggcttctt
    31981 cttttcttgc ttatttgata actatcaaaa aagtagaaga ttaagagaaa tatagaaaga
    32041 gatttcatga aacaattttt agaacgggcc agcattttgg ctctctccct cgttttgatt
    32101 acctcctttt ccatttcgag tgccctacca gccatgtttg actattatca gggttattct
    32161 aaggaacaaa ttgagctctt ggtgagcttg ccttcctttg gaatcatgat gatgttactg
    32221 ctaaatggtt tcttagaaaa aatatttcct gagcgcttac agattagttt gggcttgctg
    32281 attttatcat tgagcggtac agctcccttc tggtaccaag cctatccctt tgtctttgga
    32341 acacggcttc tctttggttt gggtcttggg atgatcaatg ccaaggccat ttctattatc
    32401 agtgaacgct accaaggaaa aaggcgaatt cagatgttag ggctacgcgc ttctgcagag
    32461 gtcgttggag cttctctcat taccttggcc gtcggtcagt tgttggcctt tggttggaca
    32521 gctatctttc tagcctatag tgctggattt ttggtgatgc ccctttatct gctctttgtc
    32581 ccttatggaa aatcaaagaa agaagtcaag aaaagagcga aggaagcaag tcgtttaact
    32641 cgagaaatga aaggcttgat ttttacctta gctatcgaag cggcagttgt agtttgtacc
    32701 aatacagcta ttaccatccg tattccaagt ttgatggtgg aaagaggatt gggggatgcc
    32761 cagttatcta gttttgttct tagtatcatg cagttgatcg ggattgtggc tggggtgagt
    32821 ttttctttct tgatttctat ctttaaagag aaactgctcc tctggtctgg tattaccttt
    32881 ggcttggggc aaatcgtgat tgccttgtct tcatccttgt gggtggtagt agcaggaagt
    32941 gttctggctg gatttgccta tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa
    33001 cgaattccag ctaaactcct caatcaagca acttcatttg ctgtattagg ctgtagtttc
    33061 ggagccttta cgaccccatt tgttctaggt gcaattggct tactaactca caatgggatg
    33121 ttggtcttta gtatcttagg aggttggttg attgtaatct ctatctttgt catgtaccta
    33181 cttcagaaga gagcttagga ttgattccta agtttttctt ttgatactca atgaaaatca
    33241 aagagcatag ttgattgagt ttggaatagt acgctgtagc tgctaaaata tttctagaaa
    33301 ttaatttgac ttttctaata gagttattca tatcttattt caatctacta tagaaagcta
    33361 gccgcaggtt gctcaaaata gtgttttgag gttgcagatg gaaactgacg tggtttgaag
    33421 agattttcga agagtatgag atttttatac ccatacaata tttgttttag gtccttgttt
    33481 acagactaat ttcttgataa aatagaggtt atgacgagat ataaagcaac tatttcctat
    33541 gatggttatg cctttgctgg ctttcagcgc cagtctcatg cgcgtagcgt tcaggaagaa
    33601 attgaaaaaa ccttgaccag attaaataaa gggcaaacca ttactgttca cggtgctggt
    33661 aggacagata gtggggttca tgccctggga caggtcattc attttgacct gccttatcag
    33721 atggatgagg agaaactccg ttttgccttg gatacccagt ctcctgaaga tattgatgtg
    33781 atttcgattg agcttgtggc agatgatttt cattgccgtt atgccaaaca tagcaagacc
    33841 tatgagttta ttgtggatag aggacgtccc aaaaatccta tgcgccgtca ctatgccacc
    33901 cactttccct acccactcga tgtggaacga atgcagattg caatcaaaaa gctagaggga
    33961 acccatgatt ttaccggttt tacagcctct gggactagtg tagaggataa ggttcgcacc
    34021 atcacagaag ctagtttaat agtcgatgag acaggacaat ttttgacctt taccttttca
    34081 ggaaatggtt tcttgtataa acagattcgc aatatggtgg ggactcttct caaaatcggt
    34141 aacaaccgca tgccagtaga gcagattgat ctcatcttgg agaagaagga caggcaactt
    34201 gcaggtccca ctgcagcacc aaatggtttg tatttaaagg agattcgtta tgaagaataa
    34261 tcgtatttta gcactttctg gaaatgatat ttttagtggt ggtggactgt cagctgattt
    34321 ggctacctat accttgaacg gcttgcatgg gtttgtagca gtgacttgtt tgacagcctt
    34381 gacagaaaaa ggatttgaag tctttccaac tgatgatacc atttttcaac atgaattaga
    34441 tagcttacgt gatgtggaat ttgggggaat taagattggt cttctcccta ctgtcagtgt
    34501 ggctgagaag gccttggact ttatcaaaca acgcccagga gtacctgtgg tgttggatcc
    34561 tgtcttggtc tgcaaggaaa cgcatgatgt agctgtcagt gagctctgcc aagagttgat
    34621 tcgctttttc ccttatgtca gtgtgattac gcctaatctc ccagaagcag aattattatc
    34681 cggtcaggaa attaaaacct tggaagacat gaaaactgca gcgcagaaat tgcatgattt
    34741 aggagcgcca gcagtcatta tcaagggagg caatcgtctt agtcaggaca aggctgtgga
    34801 tgtcttttat gatggacaga cctttactat cctagaaaat ccagttatcc aaggccaaaa
    34861 tgctggtgca ggttgtacct ttgcctctag cattgccagt cacttgatta aaggtgataa
    34921 acttttgcca gcagtagaaa gctctaaggc tttcgtttat cgtgctattg cacaagcaga
    34981 tcagtatgga gtaagacaat atgaagcaaa caaaaacaac taaaatcgcc cttgtatccc
    35041 tattaaccgc cctttctgtg gttctaggtt atttcttaaa aatcccaaca cctacaggaa
    35101 ttctaactct tttagatgct ggtgtcttct ttgcggcctt ttactttggt agtcgtgaag
    35161 gagcggtagt cggaggacta gcaagtttct tgattgacct cttatcaggc taccctcagt
    35221 ggatgttctt tagcttggtc aaccatggct tgcagggatt tttcgcagga tttaaaggaa
    35281 aaagtcagtg gttaggcctt attttagcaa ctattgccat ggtaggaggc tacgccttgg
    35341 gttctacttt gatgaatggc tgggcagcag ccctcccaga aattctacca aatttcatgc
    35401 aaaatatggt agggatgatt gtaggattta ttcttagtca aagtatcaag aagattaagt
    35461 aatactcttc gaaaatctct tcaaaccacg tcagcttcca tctgcaacct caaaacactg
    35521 ttttgagcag cctgaggcta gctttctagt ttgcactttg attttcattg agtataaaga
    35581 ggttgagtaa aaagtatttc aaataagaaa aacgcatagt atcaggtgtt gaatgaactg
    35641 ccccccaaaa gttagacaga aaaaatctaa cttttggggt gttttattat gaaactaact
    35701 tatgatgata aagttcagat ctatgaactt agaaaacaag gatatagctt agagaagctt
    35761 tcaaataaat ttgggataaa caattctaat cttaggtaca tgattaaatt gattgatcgt
    35821 tacggaatag agttcgtcaa aaaagggaaa aatcgttact attctcctga tttaaaacaa
    35881 gaaatgattc ataaagtctg acatgaaggc tagactaaag atagagtttc tcttgaatac
    35941 ggtctcccaa gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat
    36001 actattgttg agaaaacaag agggagagta cctaaaatgg gacgtaagcc aaaaaagaga
    36061 cctgaagaga ggacagaatt agaacgtctt caagcagaaa atgagtacct gagagcggag
    36121 aatgccatcc taaaaaagtt aagagaactc cgattgaagg aggaaaaaga gaaagacaga
    36181 aattgtttaa gaattaatga ctgagttttc gttagatctt cttctaaaag ccattaaact
    36241 agctcgttcg acctactact atcacttgaa acagctagac aaaacagata aggaccaaga
    36301 gcttaaagct gaaattcaat ccatttttat cgaacacaag ggaaattatg cttatcgtcg
    36361 aattcattta gaactaagaa atcgtggtta tctggtaaat cataaaagag ttcaaggctt
    36421 gatgaaagta ctcaatttac aagctaaaat gcgacagaaa cgaaaatatt cttctcataa
    36481 aggagacgtt ggtaagaaag cagagaatct cattcaaggc caatttgaag gctctaaaac
    36541 aatggaacag tgctacacag atgtgactga atttgccatt ccagcaagta ctcaaaagct
    36601 ttacttatca ccagttttag atggctttaa tagcgaaatt atcgcctata atctttcaac
    36661 ttcacccaac ttagaacaag tacaaacaat gttggaacag gcattcacag agaagcacta
    36721 cgagaatacg attctccata gtgaccaagg ctggcaatat caacacgatt cttatcatca
    36781 gtttcttgag ggtaagggaa ttcaagcatc catgtcacgc aagggaaaca gccaagacaa
    36841 cggtatgatg gagtccttct ttgggattct gaaatcggaa atgttttacg gttatgagaa
    36901 gtcgtttcag tcgcttaagc aattggaaca agctattgta gactatattg attactacaa
    36961 caataaacga attaaggtaa aactaaaagg acttagtcct gtgcaataca gaactaaatc
    37021 cttcggataa attatttgtc taactgtttg ggggcagtac attttaacac ttgatactat
    37081 gcgttttatt gtgggaagat tttcctcatt ttctagtgaa attgaacttt ttctaacatc
    37141 ttttgataga tgacttctta atcaattatc atttccattg ttgtttcttg gggtttcatc
    37201 ccttgtcttt gataaaagcg gagagcaccc tcgttatcat tccagacact tagggttaga
    37261 ttattgcagc caatttcttt agcataatag cagaaaaatg acaataaaat agcccctaga
    37321 tattttgaaa ggaaagcata ggggctatat agtttaatca aaataaagca aatctttgct
    37381 ggtgcttgta aagaggtcga agccatcctt ggtaacaaca ccgcagtctt caatacgaac
    37441 accgacttta ccagggatat agatacctgg ttcaacagag aagcacatgc cttcttcgat
    37501 gaccatgtcg tttccttcca tgatagatgg gaattcatgg acatccatac cgataccatg
    37561 cccgagacgg tggttgaagt actcaccata accagctttt tcgatgacct cacgggcagc
    37621 gcggtccact tcatgagcag tcacacctgg cttgataaag tcaagagcag cttgttgggc
    37681 ttcaagagtc aagttgtaaa tatctttctt gaattggtct ggtttgccga cagcgactgt
    37741 acgagtcata tctgacgcat agccattgac cagaacaccc aggtcaaaga ggagaagagc
    37801 atcattttca accttattag ctgctggaat gccgtgtgga ttcgcagcat tatcaccagt
    37861 caagaccatg gtatcaaagc tcatttcata accttcacgt ttcatggcaa agtcgatttg
    37921 tgcgatgata tctgtctcag tcttatcaag agaaatattg tcaaaaccaa catgaacagc
    37981 cttgtcagca taaagacctg caaccatcat tttttgcact tcatcagctg atttgatgag
    38041 gcgcatgcgt tggatacgag gagtgaggtt gtcaaactca gcagtctcaa aaactgtttt
    38101 caaaccatgg tatttggtca agatgagatt gtcaaactca acagcgacac gtttgaagtc
    38161 aagttgtgga agagcatgtt tgattttttg ccatggattt tcagaatcga catagcccac
    38221 tactgggaag gaaacggtgc tacttgcacg ttctacttca agagctggga caaagaggag
    38281 aggttcctga tccgctagga caaagaggaa catttggcgt tcatggggat cactgtaaaa
    38341 accagtgagg taattgattg tgacggggtc agatacgaca gcgacgtcta gtttttctga
    38401 ttcaagatat gttaggattt gttgtaattt agacatatgc taccttcttt ctacccctct
    38461 attttggcaa aaaatgagag aaaatgcaag ggagaagggt aaaagaaaag acaaaaaact
    38521 ccgacaagat aacggagttc tttgatatca ttttcctttg tttaagaaaa gtcagccttg
    38581 cttttgacgt cactgtactc ttcagcgatt tcttggctga gaatgtcctc ataggcaggg
    38641 agttggatgt cctgaccata tttcttcttg agggcagtag taactaggcg ataagccaga
    38701 ttggcattac gagagaggat aggcccgtgg aagtaggaac caaagacatt cttataatga
    38761 accccttcac cgaccttttc ttcgttgttt ccatttccat agacaacctg tcccagcggt
    38821 ttttggtcat cagagaggaa ggtacgacct tggtgatttt caaatccata gtaggtttca
    38881 tcgaaatctt cattgtgaat cttgatgtca ccgataaaac ggttattggt ctggttgagc
    38941 gtgtagtgtc ccatgacccc tagcccttcg atacgttttc ctgaagcttc aacataatat
    39001 tgacccaata gttggaaacc accgcagata gccagaacta caccgtcgtt ttggatgtag
    39061 ttgtcaatgc tctctttttt agcaggtagg tcgtctgcaa tgatactttg ttcaaagtct
    39121 tgaccaccac cgaaaaaggc gatgtcgtag tgattttcat caaagtcatc atggagagaa
    39181 acgatgtcaa cggtcacatg ggctcccagt ttttcagcca catacttgag catgaggatg
    39241 tttccattgt ccccgtaggt attcatgaga tttccgtaga ggtgggcaat gttgagctga
    39301 taggggtaat tgctatcttt tgaggaaagt gaagtataaa ccattagttc atctcctttc
    39361 taacaatctg acgactagcc agcagttcac gaaattccag catggcagta taagttgcca
    39421 gaatataggc atgcttgcag tcttgattct caatggtctt gagaacttgc tccagattac
    39481 tcgtttcagt gattttctca gctggatagc cagtcactcg gaggcgacga gcgatttcag
    39541 aatgacgaac accgccagcg ttgatttcag gaatgtccat gtcagtgatt tgctcaaagt
    39601 ctgcatccca gatccagcta gtgtcaattc catctgcata gttggcatta aggaggacag
    39661 ataggctaaa tggataaggt gctagtttga tcatttcgat agcttgggtt gcaccgactg
    39721 gatttttaat caagacaagg gtacattcct tgtcaccgat atgaaaggtt tcttggcgtc
    39781 caaagacagc acggctcttg tcaaatccct gtttgatgag ttgggaatct gcgccgagga
    39841 aacgggcgat ggccacagca gctagggcgt tatagatatt atagagcccg ccgatttgga
    39901 taccgtattc ttggccgtct atgacaaagc gagagcgatt gttggtcaac tcaaccagtt
    39961 ttgtcaaacg atagtcgaga tcaggacgtt tacatccaca accttcacag atataggcac
    40021 ccaagtttgc ataggtatta tgctcatatt tgaggatgcc ttggcagtca ggacagagaa
    40081 tcccttcggt attgtagtga gcaagttggg ctggtccttt ttccaagtca aaaccaaaat
    40141 actctatagg gtttggaata gttggcttgt agaaaagtgg actgtctccg ttaaggagaa
    40201 cagtagcagt tggaactttt cgaatggcat ccaatatcat gttataggta gtatagattt
    40261 caccgaaacg gtccatctgg tcacggaaga tattagtaat gacaaaaaga ctaggctgga
    40321 tatagtcaca gatacgagat agactggctt cgtcaatttc gaggacggca atatttttcc
    40381 cagtttttga agatttggct gttaggaagg ttgttgcaat ccctgtaatc atgttggcac
    40441 cgcttgggtt ggttagaact tgaccataaa cctcttttaa aatgccgaca gtgagggcag
    40501 ttgtcagggt ttttccattt gttccagtga caacgacaat ctcgtagttc ttagctaggc
    40561 tttgtaaaat atctttatca aattgaaggg cgactttccc tgggagcgta cttccacgtc
    40621 caagacggct taaaacgaag tgggaagaac gcccagcaag aaggcccaaa gtagttttta
    40681 agttcatgtt tctattatat cacaaaagag ggaaaagaca gatttggtgt ttcgtagaag
    40741 taaaaacagc ccaaagaggg ctgtcagtta agatgagatt tcaacttaaa tcgcaaacaa
    40801 gtcattcaag ttctcagcca aggttgggtg agtgaagatt tgttttgtga agtaagtgta
    40861 aggaatcttg ttgtccatag caacagtaat gatgttgatg atttcttgag aaccttctga
    40921 gaagatgctt gcaccaagaa tttcttttgt ttcagtatta acaacagctt tgaaagctcc
    40981 gcgaaggtct ccatttacgt gaccacgagg catggctgca acagggattt ctttcactgc
    41041 gtatggaagt ttcaaatcag ctgcttggct ttcagtcaaa ccaacttgtg aaagtgcagg
    41101 tgtgatgaac atagtatttg gcacattgag acggtcctca agtgtgtagc tgccatctcc
    41161 agcaaggtag ctgtaaacaa cacggaagtc atcaagtgaa atgtaagtaa attgaaggcc
    41221 accgttgaca tctccaactg caaagacacc aggaacgttt gtttgacagt gtttgtctac
    41281 tttaatagca ccacgttcag ttagttcaat atctgtattt tcaagttgaa gtggttctac
    41341 atttggtttg cgtccagttg cgtagagaag ggcgtcgaaa cggtaagttt cgtcttcagt
    41401 tacgacaagc acttggtcac catcgttttt gatttcagta gtatggatat tttgaagcaa
    41461 ttcaatgcca tcttcttcca tgtattgttt agcaagagct gcgatggaag gttctgcacg
    41521 aggtaggaat gtatccaagg catctaggac tgtgaccttg cttccaagtt tgttgtaaag
    41581 gccggcaaat tcaagaccga tatttccgcc accaaggatt ccaagttttt caggtaattt
    41641 gtccaagctt tggatacctg ttgagtcaaa gatgtttttg cttgtagcaa gtccaggaat
    41701 tggcaagacg tttgaaacag caccagtgtt gatgacgatt gtttcagcag tcagttcttt
    41761 cttttcatca ccagcttgga tttcgatgac tttatttgaa aggaagtgag cttccgcatc
    41821 aaagatatct acgcctgtac cagcaacagt cgcatagttt ttaccgttga ggcgaccagt
    41881 gatcgtgttt ttagtagcaa tcacttcttc aaaagacaag tccttttcag cagcaactag
    41941 caaggtttta gttgggatac aaccaatgtt gatacaagtt ccaccgtaca tagccttgct
    42001 gcgttcaacg agggcaactt ttttgccagc tgaagccaat ttacctgcta gtgttttacc
    42061 agctttacca aatccaataa caattaaatc gtatgttaac atttatcttc ctcctattcg
    42121 aatttcattc tagcacaaga aaaaaacttt tgcacaattc tgctacggaa aagacagaaa
    42181 aagataaaaa cgctttccag acaaaaaact agtaattttt tgacaagctt gataaactaa
    42241 aatcctatct atttttatat aagaaagaat aaatgttcga aaataggagg ttttatgaaa
    42301 tcgaatcgtt atattattgc ctttgctggg gtcattttac acttaatgct gggttcgact
    42361 tatgcttgga gtgtttatcg taaccctatt attgaaaaaa cgggatggga tcaggcttct
    42421 gttgccttcg cctttagtct agcaatcttt tgtttgggct tatcggctgc atttatgggg
    42481 cgtttggtag aaaaatttgg tccgaaagtc atggggagtc tatctgcttt tctatacgca
    42541 ggtggaaata tcttaacagg atttgcaata gaccgtcagg aactgtggtt gttgtatcta
    42601 gcttatggca ttttaggtgg gcttggtttg ggagcaggct atattacccc tgtgtcaacg
    42661 attataaaat ggtttcctga taaacgtggt ctcgcaacag gtttagcgat tatggggttt
    42721 ggttttgctt ctttattgac tagtcccata gcgcaacacc tcatcgcagg ggtagggatt
    42781 gtagaaactt tttatatttt aggagcaagt tactttatta tcatgctcct agcttcacaa
    42841 ttcattaagc gtccaaatga gcaagagctt gcaattttat cttcttcagg gaaagaaaaa
    42901 acagcctctt tgacgcaagg aatggctgca aatcaggctc taaaaagcaa tcggttttat
    42961 atgctttgga ttattttctt tatcaacata gcttgtggtt taggcttaat ttcagcggca
    43021 tcgccaatgg cacaggagat ggctggcttg tctacaagtc atgcagcagt aatggtgggt
    43081 gttttgggga ttttcaatgg atttggtcgc ttgctctggg cgagtttgtc tgactatatc
    43141 ggtcgccctc taacctttag tatattactg cttgtcaatc ttttcttttc tctctcactt
    43201 tggctcttta cagattccgt tttatttgta gttgctatgt ctattttgat gacttgctat
    43261 ggagctggtt tttctttgat tccagcttat ctcagtgata tttttggaac caaggaattg
    43321 gccgctctgc atggttatat tttaacagct tgggcaatgg ctggtttagc gggacctatt
    43381 ttattagcag agacttataa aatggctcat tcgtacacac aaaccttgtt cgtttttctc
    43441 attttataca gtatcgcctt ggctttgtct tattatctag gtcgttcaat caaaaaagaa
    43501 agtcaaaaac cgcttacatg attttctgta gaaatggggc tatcttttgc tctggtcttg
    43561 tgttatacta gataggttgc aaagaaaaca gtacttttct tttgtggaaa agaagcaaca
    43621 cgattttata tctagttata tgaaaatacg tcataaaaag aaaagtataa actaagccct
    43681 atagggtggc ttcgcaccac ctttagaaag aagaataacg tgaaatttaa tgaattaaac
    43741 ttgtctgctg atttgctagc agaaattgaa aaagctggtt ttgtagaagc tagtcctatc
    43801 caagaacaaa ctattccttt ggcccttgaa ggcaaggatg ttatcggtca agctcagact
    43861 ggtacaggaa aaactgcagc ctttggcttg cctacccttg aaaaaatccg tatagaagaa
    43921 gcgactattc aagccttggt catcgctcca actcgtgaac tagctgtcca aagtcaagaa
    43981 gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc gttcagtata tggcggatca
    44041 agcattgaaa aacaaattaa ggctcttaaa tctggtgccc atatcgtggt gggaactcca
    44101 ggtcgcctct tggacttgat taaacgcaag gccttgaaat tacaagacat tgaaaccctt
    44161 atccttgacg aagcggatga aatgcttaac atgggcttcc ttgaagacat cgaagccatt
    44221 atttcccgtg tacctgagaa ccgtcaaact ttgcttttct cagcaactat gccagatgcc
    44281 atcaaacgta tcggtgttca gtttatgaaa gcccctgaac atgtcaagat tgcggctaag
    44341 gaattgacaa cagaattggt tgaccagtac tatatccgtg ttaaggaaca agaaaaattt
    44401 gacaccatga ctcgtctcat ggatgtggca caaccagaac tcgctattgt atttggtcgt
    44461 accaaacgcc gtgtggatga attgactcgt ggtttgaaaa ttcgtggctt ccgtgcagaa
    44521 ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg tccttcgtga ctttaaaaat
    44581 ggcaatcttg atgttttggt tgcgacagac gttgcagcgc gtggtttgga tatttcaggt
    44641 gtgacccatg tctacaacta cgatattcca caagatcctg agagttatgt tcaccgtatc
    44701 ggtcgtacag gtcgtgctgg taagtcaggt caatctatta cttttgttgc tccaaacgaa
    44761 atgggttacc ttcaaatcat tgaaaacttg actaagaaac gcatgaaagg tctcaaacct
    44821 gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag ctctcaagaa aatcgaacgt
    44881 gattttgcag atgaaaccat tcgtgccaac tttgagaaat ttggtaagga tgctcgcaaa
    44941 ttggctgctg agtttactcc agaagaattg gcaatgtata tcttgagtct gacagtccaa
    45001 gacccagata gccttccaga agtggagatt gcacgtgaaa aaccactacc gtttaaacca
    45061 tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg gaggccgtcg tggggacgac
    45121 cgtcgagagc gtgatcgtcg tggcaatggt cgccgtgatg agttcaaaaa aggaagtcgt
    45181 ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg ataataaaaa accacgcaat
    45241 actttaagcg aaaagcaaac aggctttgtt attcgtaaca aaggtgataa ataagaaaaa
    45301 gcggttcaaa atgaatcgct tttttatata aggagaccga atgtgaaaaa gacaaataaa
    45361 attattatat tatcttttat aatattatga tacacaaaaa taggcgactt gtcaatagaa
    45421 aaggaacaat ttaattaaaa atattctggt ttttaattaa attgtaagtc aatgcaagtc
    45481 aataaggaat tttcagataa ttattgaaaa agagacgttt ttatgttata atgatagcga
    45541 ttaaattcga ggtgaaaaat ggcacattta ttagaaaaaa ctagaaaaat tacatcaatt
    45601 ttgaaacgct cagaggagca gttgcaggat gagctccctt acaacgctat tacgcgtcag
    45661 ttagcggata ttattcattg caatgcctgc attatcaata gtaagggacg tctgcttggc
    45721 tattttatgc gttataaaac aaatacagat cgcgtagagc aattcttcca aactaagatt
    45781 ttcccagatg actacgttca aggggctaat atgatttacg aaacagaagc aaacttgcct
    45841 gttgagcatg atatgagtat tttccctgtt gagagtagag atgattttcc agatggcttg
    45901 acgactattg caccgattca tgtatcgggg attcgccttg gttctttgat tatttggcgt
    45961 aatgataaaa aattcgaaga tgaggacttg gttcttgttg agattgccag taccgttgtt
    46021 gggattcagc ttcttaactt ccaacgtgaa gaagatgaga aaaatattcg tcgtcgtact
    46081 gctgtcacca tggcggtcaa taccctttct tactccgaac tccgtgctgt ttcagcaatt
    46141 ttaggggaat taaatggaaa tgaagggaag ttgactgcgt cagtgattgc agatcgtatc
    46201 ggaatcactc gctctgtgat tgtcaatgct cttcgtaaac ttgagtctgc ggggattatt
    46261 gaaagtcgct cacttggaat gaagggaacc tatcttaagg tcttgatttc agatattttt
    46321 gaagaagtga agaaaagaga ttactaatga caaaggcttt aatttcgatt gattatacag
    46381 aagattttgt tgctgatagt gggaagctga cagcaggtgc tccagctcag gcgatttcgg
    46441 atgccatcag caaggtaact cgattagctt ttgaacgagg agattatatc ttctttacta
    46501 ttgatgctca tgaagaaaac gattgtttcc atccggaaag taagttattt cctcctcata
    46561 atctgattgg gacgagtgga cggaatttat atggagattt ggggatcttt tatcaagagc
    46621 atggttcaga cagtcgtgtc ttttggatgg ataaacgcca ttactcagct ttttcaggga
    46681 ctgacctaga tattcgtttg agagagcgta gagtgtctac cgttatctta acaggtgtct
    46741 tgacggatat ctgtgtccta catacagcta tagatgccta taatctagga tatgacatcg
    46801 agattgttaa accagctgtt gcttccatct ggcctgaaaa tcatcaattt gccctaggtc
    46861 atttcaaaaa tacactcgga gctaagttag tagatgaaaa tctaaatgaa ctttctgagt
    46921 aacctggttg gtgaaaatga aaaaatttga aaaaaagtgt tgacaagcat cccaaaagtt
    46981 gatatactag taaagtaatc gatgcgggga tggcggaatt ggcagacgcg caggactaag
    47041 gatcctgtga ccgctttagg tcgtgagggt tcaagtccct ctctccgcat aggaaaagag
    47101 ttagctaaag ctagctcttt tgtatttctc taaaaaatta gaatccagtc aaatgtagtg
    47161 gagaatcagc gaaaatatgg gaaaactgat ccttgtttct gcttttgtca tagaagaata
    47221 ataaagatta tcttcttcaa attctccgat attctctaaa gttttgtgca agttgcacag
    47281 aactttttta tttttttggt catcttgcca tagaaatata aagcgttttc atctataata
    47341 taattatcaa aagacaaaag gagttcacct catggtagaa ttgaatctta aaaatattta
    47401 caaaaaatat ccaaacagcg aacactattc agttgaagat ttcaacttga acatcaaaga
    47461 taaagaattt atcgttttcg taggaccttc aggatgtggt aaatcaacta cactccgtat
    47521 gattgctggt cttgaagaca tcacagaagg tactgcatct atcgatggcg tggttgtcaa
    47581 cgacgtagct ccaaaagacc gtgatatcgc catggtattc caaaactacg ctctttaccc
    47641 acacatgact gtttatgaca acatggcttt cggtttgaaa ttgcgtaaat acagcaaaga
    47701 agacattaac aaacgtgttc aagaagcagc tgaaatactt ggattgaaag aattcttgga
    47761 acgtaaaccg gctgaccttt caggtggtca acgtcaacgt gttgccatgg ggcgtgcgat
    47821 tgtccgtgat gcgaaagtat tcttgatgga cgaacctttg tcaaacttgg atgccaaact
    47881 tcgtgtatca atgcgtgctg aaatcgctaa aattcaccgt cgtatcggag ctacaactat
    47941 ctatgtaact cacgaccaaa cagaagcgat gacacttgca gaccgtatcg ttattatgtc
    48001 agctactaag aaccctgctg gtacaggtac tatcggacgt gtagaacaaa tcggtactcc
    48061 tcaagaagtt tacaaaaatc cagttaacaa attcgttgca ggattcatcg gaagcccagc
    48121 tatgaacttc atcaacgtga aattggttgg tagcgaaatt gtttctgacg gtttccgttt
    48181 gaaagtgcca gaaggagcat tgaaagttct tcgtgaaaaa ggctacgaag gaaaagaatt
    48241 gatctttggt atccgtccag aagacgtgaa tgcagaacct gctttccttg aaacattccc
    48301 agactgtgtt gtaaaagcga ctatctctgt atcagaactg cttggttcag aatctcacct
    48361 ttactgccaa gttggtaaag acgagtttgt tgcaaaagtt gatgctcgtg actacttgca
    48421 aacaggtgca acagttgagc ttggatttga cttgaacaaa gcacacttct tcgatgtaga
    48481 aactgaaaaa acaatctact aagaataaat aaaattcaaa gcactacaag aaaagatatc
    48541 tctttatcaa ttgtagtgga gagatatcag ttaatctagg gagagaaaca aaatgcttct
    48601 ctcctttttg ctagggaagt catattatac atctatattg tgatgctctt tatactcttc
    48661 gaaaatctct tcaaaccacg tcaacgtcgc cttgccgtac gtatgattac tgacttcgtc
    48721 agttttatct gcaacctcaa agctgtactt tgagtagctt acggctagtt tcctagtttg
    48781 ctctttgatt tccattgagt attatttgtg ggtaccatct acaagtgaag ctatatgcgt
    48841 aaactacgtg agcaattgaa ttcgaactag agaggtaata ataaatttat gctatagtta
    48901 tggtgacttg tatgcttttg attctagttt atcaaataat agattagaat tgtcagataa
    48961 tatcattttg tgtcattttg tgttataatg aagaaaaaac agaggtgttc aaatgtcaga
    49021 agcaggtcat aagtttttag caaaattggg gaaaaaacgc ttacgtccag gtggaaagcg
    49081 tgccacagat tggttaattg cagaaggagg attttcaaaa gaaaagagaa tactagaggt
    49141 tgcgtgtaat aggggaacta cagcaattga gttggcacag cgttttggtt gcaagataac
    49201 tgctgttgat atggatgctc aagctttaga agtggctaaa aaatctgctg gaacggcagg
    49261 tgttgctcat ttaatcagtt ttgaaagagc aaatgcaatg aaacttcctt atcaagatgc
    49321 tagttttgat attgttataa atgaagctat gctgactatg caagccgatc aagctaagaa
    49381 aaaatgtgta atggaatatc taagggtatt aaaacctgga ggtcttctct tgacacatga
    49441 tgtgcttctt aaggaagcta aagagtctat cagacaggaa ttatcacaag caattcatgt
    49501 aaatgtaggt cctttaactc aagatggttg ggaacaggtg atgatagaat caggttattg
    49561 tgatgtgaaa gtattgactg gtgaaatgac attaatgaaa ttatcgggta tgatttatga
    49621 cgaaggttgg ctaggaactt tgaaaatttg tgtaaatgct tgtaaaaagg agaatagaaa
    49681 gcagttttta actatgtata aaatgtttgc taagaataaa cagaaattgg gctttattgc
    49741 gatggctagt tataaatcgt caaaacgtta gataattatt gaagttaact tttccttttt
    49801 tctttcttaa aaaatatgct ataatagaga gtaaaaaact ttgaaagaaa gaaaaagatg
    49861 aatttaaaag attacattgc aacaattgaa aattatccaa aggaaggcat taccttccgt
    49921 gatattagtc ctttgatggc tgatggaaat gcttatagct acgctgttcg tgaaatcgtt
    49981 cagtatgcta ctgacaagaa agtcgacatg atcgtgggac ctgaagctcg tggatttatc
    50041 gtgggttgtc cagttgcctt tgagttggga attggttttg cgcctgttcg taagccaggt
    50101 aaattgccac gcgaagttat ttctgctgac tatgaaaaag agtacggtgt cgataccttg
    50161 actatgcacg cggatgccat taagccaggt caacgtgttc ttattgtaga tgaccttttg
    50221 gcgacaggtg gaactgttaa ggcaactatc gagatgattg aaaaacttgg tggtgttatg
    50281 gcaggttgtg ccttccttgt tgaattggat gaattgaacg gccgtgaaaa aattggtgac
    50341 tacgattaca aagttcttat gcattattaa tgaaaacagt ccctagggct gttttctcta
    50401 cactaggata taaaaataga ctataactag ttagagaaaa actataattg aaaactatat
    50461 cttcttacag tataataaaa ggactaagtg tttgagattt gtcttcaaac atatgcaatt
    50521 attcctgaaa gagtacagtt aggagagggt tatgccgatt cgaattgata aaaaattgcc
    50581 agctgttgag attttacgga cagagaatat ctttgtcatg gatgatcaac gtgctgccca
    50641 ccaagatatc cgtcctttga agattttaat tttaaatctc atgccacaga aaatggtcac
    50701 agagacccag ttgttgcgcc acttggctaa tacaccccta caactggata ttgattttct
    50761 ctatatggag agccaccgtt ctaaaacaac tcgttcagag cacatggaga ccttctataa
    50821 aacttttcct gaagtcaagg atgagtattt tgatgggatg atcatcacgg gtgctccagt
    50881 tgagcattta ccatttgagg aagtggacta ttgggaggaa tttagacaga tgcttgagtg
    50941 gtctaagact catgtctatt cgacccttca tatctgttgg ggggctcagg ctgggcttta
    51001 tctgcgctat ggtgtagaaa aataccagat ggacagtaag ctatcaggta tttatcctca
    51061 ggacacccta aaagagggtc accttctatt tagaggcttt gatgatagct atgtatcccc
    51121 tcattcacgg cacacggaga tttctaagga agaggtctta aacaagacca atctcgagat
    51181 tttatcagaa ggacctcagg ttggggtttc tattttggcc agtcgtgatt tacgagaaat
    51241 ttatagtttt ggtcatttgg agtatgaccg tgataccttg gcaaaagagt attttcgaga
    51301 tcgtgatgca ggtttcgacc cacatattcc agaaaattac tttaaagatg atgatgttaa
    51361 tcaggtacct tgtctttgtt ggtcttcatc tgcagccctc tttttcagta attgggtaaa
    51421 ccatgcggtc tatcaggaga cgccttttga ctggagaaag atagaagatg atgcatctgc
    51481 atataggtat ttataagagg aattatgaca tatttagacg cttttaaatc aggtaccttg
    51541 gttttaccga gtgccctgct cttgcatttt aaggaactct ttccttctag cgacgatttt
    51601 ctggtttggc aatttttcta tttgcaaaat acgacaggct tagaagaaat atcgccaagc
    51661 cagattgctg aaaggattgg caaggaaatt tcggatgtca accagtccat ttctaatctg
    51721 acggaaaggg gactgctcca gtatcgtact atcgaattaa atggcgaaat tgaattgctc
    51781 tttgatgcta gtttggcctt ggaacgtttg gatgacctgt ttggagcagt tcattcaagt
    51841 tcagaccagc taacaccaca aaaccagctc aaggatttgg tggaaacctt ccagcaggag
    51901 ttgggacgat tgttgacgcc ttttgagatt gaggatttga ccaagacact aaaggaagat
    51961 ggaaccagtg ctgacttgat taaggaggct cttcgtgaag ctgttttgaa cggaaaacca
    52021 aactggaagt acattcaggc gattttgaga aactggcgcc atgaaggaat caagagtgtg
    52081 gctcaaattg aggccaagag agcagaaaga gaagcaagca atcctcagtt gacacaggta
    52141 tctgcagatt tcataaatgc catggatctc tggaaggatt aatccatgca agtaggcttg
    52201 aaatccgagt aagatttgca agctgtgtat aattgtgata gaataaatag aaaataaatt
    52261 gaaaaaagag gtatgtgaaa tgtcacgtaa accatttatc gctggtaact ggaaaatgaa
    52321 caaaaatcca gaagaagcta aagcattcgt tgaagcagtt gcatcaaaac ttccttcatc
    52381 agatcttgtt gaagcaggta tcgctgctcc agctcttgat ttgacaactg ttcttgctgt
    52441 tgcaaaaggc tcaaacctta aagttgctgc tcaaaactgc tactttgaaa atgcaggtgc
    52501 tttcactggt gaaactagcc cacaagtttt gaaagaaatc ggtactgact acgttgttat
    52561 cggtcactca gaacgccgtg actacttcca tgaaactgat gaagatatca acaaaaaagc
    52621 aaaagcaatc tttgcgaacg gtatgcttcc aatcatctgt tgtggcgaat cacttgaaac
    52681 ttacgaagct ggtaaagctg ctgaattcgt aggtgctcaa gtatctgctg cattggctgg
    52741 attgactgct gaacaagttg ctgcctcagt tatcgcttat gagccaatct gggctatcgg
    52801 tactggtaaa tcagcttcac aagatgatgc acaaaaaatg tgtaaagttg ttcgtgacgt
    52861 tgtagctgct gactttggtc aagaagtcgc agacaaagtt cgtgttcaat acggtggttc
    52921 tgttaaacct gaaaatgttg cttcatacat ggcttgccca gacgttgacg gtgcccttgt
    52981 aggtggtgcg tcacttgaag ctgaaagctt cttggctttg cttgactttg taaaataatc
    53041 agtaagtagc aaaagctagg tggaacagca ttcagatgtc tgttacattt tttataggag
    53101 aggaaagatt gaaaacaaaa attggattag caagtatctg tttactaggc ttggcaacta
    53161 gtcatgtcgc tgcaaatgaa actgaagtag caaaaacttc gcaggataca acgacagctt
    53221 caagtagttc agagcaaaat cagtcttcta ataaaacgca aacgagcaca gaagtacaga
    53281 ctaatgctgc tgcccactgg tatggggatt attatgtaaa ggatgatggc tctaaagctc
    53341 aaagtgaatg gatttttgac aactactata aggcctggtt ttatattaat tcagatggtc
    53401 gctactcgca gaatgaatgg catggaaatt actacctgaa atcaggtgga tatatggccc
    53461 aaaacgagtg gatctatgac agtaattaca agagttggtt ttatctcaag tcagatgggg
    53521 cttatgctca tcaagaatgg caattgattg gaaataagtg gtactacttc aagaagtggg
    53581 gttacatggc taaaagccaa tggcaaggaa gttatttctt gaatggtcaa ggagctatga
    53641 tgcaaaatga atggctctat gatccagcct attctgctta tttttatcta aaatccgatg
    53701 gaacttatgc taaccaagag tggcaaaaag tgggcggcaa atggtactat ttcaagaagt
    53761 ggggctatat ggctcggaat gagtggcaag gcaactacta tttgactgga agtggtgcta
    53821 tggcgactga cgaagtgatt atggatggtg ctcgctatat ctttgcggcc tctggtgagc
    53881 tcaaagaaaa gaaagatttg aatgtcggct gggttcacag agatggtaaa cgctatttct
    53941 ttaataatag agaagaacaa gtgggaaccg aacatgctaa gaaaatcatt gatattagtg
    54001 agcacaatgg tcgtatcaat gattggaaaa aggttattga tgagaacgag gtggatggtg
    54061 tcattgttcg tctaggttat agcggtaaag aagacaagga attggcgcat aacattaagg
    54121 agttaaaccg tctgggaatt ccttatggtg tctatctcta tacctatgct gaaaatgaga
    54181 ccgatgctga gaatgacgct aaacagacca ttgaacttat aaagaaatac aatatgaacc
    54241 tgtcttaccc tatctattat gatgttgaga actgggaata tgtaaataag agcaagagag
    54301 ctccaagtga tacagacact tgggttaaaa tcatcaacaa gtacatggac acgatgaagc
    54361 aggcgggtta tcaaaatgtg tatgtctata gctatcgtag tttattacag acgcgtttaa
    54421 aacacccaga tattttaaaa catgtaaact gggtagcggc ctatacgaat gctttagaat
    54481 gggaaaaccc ttattattca ggagaaaaag gttggcaata tacctcttct gaatacatga
    54541 aaggaatcca agggcgcgta gatgtcagcg tttggtatta agcgatgatt tgaaagaggg
    54601 atgtgatagt agcaccctct ttttctttgt tttatgatag ttcatcctcg agtaaattca
    54661 agatcttgct tggaaatgaa gcttatatag tagattgaat atagacaaat accttgtgat
    54721 tggtaaaaca ttttagaaat tcatttacct ttcctaatcg acttggtttc atcttatttc
    54781 aatctattat agtattgggg aatttcttca aaccacatca gcttggtcag ttctacctgc
    54841 gacctcaaaa cttgtgcttt ggtcaaactg ggtttagttt cctagtttgc tgatggattt
    54901 ccattgacta taagcatcca accctctttt tgtcttctaa agaattctta aattatcagt
    54961 ctattgcaac ttttctcata taagttcttt gtcttgctat tggttttcct tagtagtata
    55021 ctaaggtagt aatcattaag aagtggttac aaaaaataat gaatgaggta aagaaaatgg
    55081 tagaattgaa aaaagaagca gtaaaagacg taacatcatt gacaaaagca gcgccagtag
    55141 cattggcaaa aacaaaggaa gtcttgaacc aagctgttgc tgatttgtat gtagctcacg
    55201 ttgctttgca ccaagtgcac tggtatatgc atggtcgtgg tttccttgta tggcatccaa
    55261 aaatggatga gtacatggaa gctcttgacg gtcaattgga tgaaatcagt gaacgcttga
    55321 ttacactcgg tggaagccca ttctctacat tgacagagtt ccttcaaaat agtgaaatcg
    55381 aagaagaagc tggtgaatac cgtaatgttg aagaaagctt ggaacgtgtt cttgttatct
    55441 accgttactt gtcagaactt ttccaaaaag gtttggatgt cactgatgaa gaaggtgacg
    55501 atgtgacaaa cggtatcttt gcaggcgcta aaactgaaac agataaaaca atttggatgc
    55561 ttgcagccga acttggacaa gcacctggtt tgtaagaaat aaggaaacat agataaatct
    55621 gtaggaaatt tcttacagat tttttctatt ttgtaagcta ttccaaacca gtcttttttt
    55681 gagttttttg ataaaatagt actatcagtg aaaaggatgg aagcatgact aagaaaatcg
    55741 tagctatttg ggcccaggat aaagagggtg tgattggtaa ggaaaatcgt ctgccttggc
    55801 atttgccagc agaattgcag cactttaaag aaacaactct gaatcatgct atcttgatgg
    55861 ggcgtgtgac ctttgatggg atggggcgtc gcttgcttcc aaaacgggaa accctgattt
    55921 tgacgcgtaa tccggaagaa aagatagatg gggttgctac ttttcaggac gtccagtctg
    55981 ttcttgattg gtatcaggct caagaaaaga atctctacat tatcggtggg aagcaaattt
    56041 ttcaggcttt tgaagcttac cttgatgaag tgattgtcac tcacattcat gctcgggtgg
    56101 aaggagatac ctatttccct ggagagcttg acttgtctct ttttgagaca gtttcaagca
    56161 aattttacgc caaagatgag aagaatcctt atgattttac catccaatac cgcaagagaa
    56221 aggaagtcta atggaacgga gtttatttgg tctattcacg gcttttctgt gtttcatctg
    56281 ctttctggca ggagcacaag cttttcgtaa aaagcgttac gggctttcta tcctcctttg
    56341 gttaaatgcc tttaccaatc tggtcaatag tattcatgct ttttacatga ccttatttta
    56401 gatagaatga cagtatagaa tggaaggaaa tcatgtctac aaatagaaaa aatgatatga
    56461 tggtttattg ctcattttgt ggcaaaaacc aagaagaagt acaaaaaata attgctggca
    56521 acaatgcttt tatttgtaat gaatgcgtgg agttagctca ggaaatcatt cgagaagaat
    56581 tggttgagga agtcttggca gacttgtctg aggtgccaaa accaattgaa ctcctccata
    56641 tcttgaacca ctatgtaatt ggtcaagatc gtgccaagcg tgccttggca gtggcggttt
    56701 ataatcacta caaacgcatc aatttccacg atacacgcga agagtcagaa gatgtggatt
    56761 tgcagaagtc aaacattttg atgattggcc caactggttc agggaaaact ttccttgccc
    56821 agaccttggc taagagcttg aatgtgcctt ttgctattgc ggatgcgaca gctctgacgg
    56881 aggctggtta tgtgggtgag gatgtggaaa atatcctcct caaactcttg caggttgctg
    56941 actttaacat cgaacgtgca gagcgtggca ttatctatgt ggatgaaatt gacaagattg
    57001 ccaagaagag tgagaatgtg tctatcacac gtgatgtttc tggtgaaggg gtgcaacaag
    57061 cccttctcaa gattatcgag ggaactgttg ctagcgtgcc gcctcaaggt ggacgcaaac
    57121 atccacaaca agagatgatt caagtggata caaaaaatat cctcttcatc gtgggtggtg
    57181 cttttgatgg tattgaagaa attgtcaaac aacgtctggg tgaaaaagtc atcggatttg
    57241 gtcaaaacaa taaggcgatt gacgaaaaca gctcatacat gcaagaaatc atcgctgaag
    57301 acattcaaaa atttggtatt atccctgagt tgattggacg cttgcctgtt tttgcggctc
    57361 ttgagcaatt gaccgttgat gacttggttc gcatcttgaa agagccaaga aatgccttgg
    57421 tgaaacaata ccaaaccttg ctttcttatg atgatgttga gttggaattt gacgacgaag
    57481 cccttcaaga gattgctaat aaagcaatcg aacggaagac aggggcgcgt ggacttcgct
    57541 ccattatcga agaaaccatg ctagatgtta tgtttgaggt gccgagtcag gaaaatgtga
    57601 aattggttcg catcactaaa gaaactgttg atggaacgga taaaccgatc ctagaaacag
    57661 cctagaggtg actatggaac ttaatacaca caatgctgaa atcttgctca gtgcagctaa
    57721 taaatcccac tatccgcagg atgaactgcc agagattgcc ctagcagggc gttcaaatgt
    57781 tggtaaatcc agctttatca acactatgtt gaaccgtaag aatctcgccc gtacatcagg
    57841 aaaacctggt aaaacccagc tcctgaactt ttttaacatt gatgacaaga tgcgctttgt
    57901 ggatgtgcct ggttatggct atgctcgtgt ttctaaaaag gaacgtgaaa agtgggggtg
    57961 catgattgag gagtacttaa cgactcggga aaatctccgt gcggttgtca gtctagttga
    58021 ccttcgtcat gacccgtcag cagatgatgt gcagatgtac gaatttctca agtattatga
    58081 gattccagtc atcattgtgg cgaccaaggc ggacaagatt cctcgtggta aatggaacaa
    58141 gcatgaatca gcaatcaaaa agaaattaaa ctttgaccca agtgacgatt tcatcctctt
    58201 ttcatctgtc agcaaggcag ggatggatga ggcttgggat gcaatcttag aaaaattgtg
    58261 aggaaaagaa aatggcaaaa acaattcata cagataaggc cccaaaggct atcgggccct
    58321 atgttcaagg aaaaatcgtt ggcaaccttt tgtttgctag cggtcaagtt cccctatccc
    58381 ctgaaactgg ggaaattgta ggagagaata tccaagaaca gacagagcaa gtcttgaaaa
    58441 acatcggtgc tattttggca gaagcaggaa cagactttga ccatgttgtc aaaacaactt
    58501 gtttcttgag cgatatgaac gactttgttc cttttaatga ggtttaccaa acggccttca
    58561 aagaggaatt cccagctcgt tcagctgtgg aggtagctcg tcttcctcgt gatgtaaaag
    58621 tcgaaattga agtcatcgca gagattggat aagctagttg aagtttggtg ttgccaaact
    58681 tcttttgata taaggagaaa aagatgacaa agaaacaact tcacttggtg attgtgacag
    58741 ggatgagtgg cgcagggaaa actgtagcca ttcagtcctt cgaggatcta ggttatttca
    58801 ccattgataa tatgccgcca gctctcttgc ctaagttttt gcagctggtt gaaattaagg
    58861 aagacaatcc taagttggcc ttggtagtgg atatgcgtag ccgttctttc ttttcagaga
    58921 ttcaagctgt tttggatgag ttggaaaatc aagatggttt ggatttcaaa atcctctttt
    58981 tggatgcggc tgataaggaa ttggtcgctc gttacaagga aaccagacgg agtcacccac
    59041 tagcagcaga cggtcgtatt ttagatggaa tcaagttgga acgtgaactc ttggcacctt
    59101 tgaaaaatat gagccaaaat gtggtggata cgactgaact cactccacgt gagctgcgca
    59161 aaacccttgc agagcagttt tcagaccaag aacaagccca gtctttccgt atcgaagtca
    59221 tgtctttcgg atttaagtat ggaatcccga ttgatgcgga cttggtcttt gatgtccgtt
    59281 tcttgccaaa tccctattat ttaccagaac tgagaaacca aacgggtgtg gatgaacctg
    59341 tttatgatta tgtcatgaac catcctgagt cagaagactt ttatcaacat ttattggcct
    59401 tgattgagcc gattctgcca agttaccaaa aggaaggtaa gtccgttttg accattgcca
    59461 tgggatgtac gggtggacaa caccgtagtg tggcatttgc taaatgcttg gcgcaggact
    59521 tatccaagaa ttggtctgtt aatgaagggc atcgcgacaa agaccgcaga aaggaaacgg
    59581 taaaccgttc atgagaaaac caaagataac ggtgattggt ggagggactg gaattcccgt
    59641 cattctaaaa agtctgcggg aaaaagatgt ggaaatcgca gctatcgtga cggtggcaga
    59701 tgatggtggt tcttcaggtg aactccgaaa aaatatgcaa cagttgacac cgccaggtga
    59761 tcttcgtaat gtccttgtgg ccatgtcgga tatgcctaag ttttatgaga aggtctttca
    59821 gtatcggttc tctgaggatg ccggagcctt tgctggccat ccattgggaa atctcatcat
    59881 tgctggcttg tcagaaatgc agggttcaac ctataatgcc atgcagttat tgagcaaatt
    59941 tttccataca acagggaaaa tttatccttc cagtgaccat cctttgaccc ttcatgcagt
    60001 ctttcaggat gggacagaag tggctggaga gagtcatatt gtagaccatc gaggcataat
    60061 tgacaatgtc tatgtgacca atgccctaaa cgatgatacg cctctggcca gccgtcgagt
    60121 agtgcagacc atccttgaaa gtgacatgat tgtcctaggg ccaggttccc tctttacctc
    60181 tattttgccc aatatcgtga ttaaggaaat tgggcgggct cttttggaaa ctaaggcaga
    60241 aattgcctat gtctgcaata tcatgaccca acgtggggag acggaacact ttacagatag
    60301 cgaccacgtg gaagtcttgc atcgtcacct tggtcgccct tttatcgaca ctgtcttggt
    60361 gaatattgaa aaagtgcctc aggaatacat gaattccaac cgttttgatg aatacttagt
    60421 gcaagtggaa cacgattttg taggtctttg taagcaagtt tcgcgcgtga tttcatctaa
    60481 cttccttcgt ctggaaaatg gcggtgcctt ccacgatgga gatttgattg tggacgagtt
    60541 gatgcgcatt atacaggtga aaaaatgagt ttcacagtag cagtaaaaga agaaatcctg
    60601 ggccaacacc acctgagccg gcatgaatta tctgccatta tcaaaatgtc tggtagcatc
    60661 ggtctctcga cttcgggctt gactttgtca gttgtgacag aaaatgccaa actggcccgt
    60721 cacctctatg agtcctttct ccatttctat gaaatcaaat ctgaaattcg tcactaccaa
    60781 cggagcaatc ttcgcaagaa tcgcgtctat accgttttta cagatgaaaa ggtgcaggat
    60841 ttgttaagtg atttgcactt ggcagactct ttctttggcc tggaaacagg tattgatgag
    60901 gcgattttat cggacgagga agcaggtcgt gcctatctct gtggcgcttt cttggcaaat
    60961 gggagcattc gtgaccctga gtcaggcaag taccagttgg aaatcagttc tgtttatctg
    61021 gaccacgcgc aaggaattgc ctcccttctc caacagtttt tactggatgc caaggtgctt
    61081 gagcgcaaga agggggctgt gacctatctc cagcgagcag aagacattat ggacttcttg
    61141 atagtaatcg gagccatgca ggcacgtgat gattttgagc gggttaagat tttgcgagaa
    61201 acccgtaacg acctcaatcg ggctaataat gccgagacag ctaatatcgc tcggacagtt
    61261 tctgccagca tgaagaccat taacaatatc agtaaaatca aagatatcat gggcttagaa
    61321 aatttgccag tagatttgca ggaagtggcg caactgagga ttcagcaccc agactactct
    61381 atccagcagt tagcagatag tctcagcacc cctctgacca aaagtggtgt caatcacaga
    61441 ctcagaaaaa tcaataagat agcggatgaa ttataaaacc acgaatccta tgtgactcgt
    61501 ggttcttttt tataaactag tagagtgttt tggttgtact ttttgttcag ggtcaatgta
    61561 gttgatagcg ttgttgacag cagttggagc ttctccgagg cctgtcgcaa tcagatcaat
    61621 ttttccgtca tagtagcagc agtcaccgat agcatagata cctgcttggc tggattcctg
    61681 tttgctgttg acgataatct tgtgacggtt gaggtcgagc ccccagtttt taaggttacc
    61741 gacagaagat ttgaaaccat agttgacaaa gaggtggtct aggtcaatgg tttcagtttc
    61801 atcagatttg acttttgtga tttcaagttt atcaagtgtt tttccatttc caaggagttg
    61861 gctaggggcg aatggtgtct tgatggttac agatgattct tgcaaggctt gaacactgtg
    61921 ttccaaggca cggaaattat ctctgcggtg aacaagggta gttggtgcga ttttttcaaa
    61981 agccaaagcc caatccacag ccgagtctcc cccaccaaga atcgtcactt tcttaccagc
    62041 gtattgctga atgttagaaa cgtggtagtg gatattttca tagccctcaa ccccttcaag
    62101 ttccagcgga cgtggtttga aggcaccgcc acccatagcg atgataactg ttttagtcag
    62161 gtgacttcct ttagaagttg tgatggcaaa ttcttcttgt ttgtcaatct caagaaccgt
    62221 ttcattgaga tgaatagggg tatcaaatcc atttagctgt tcaatcaagc ggttagtcaa
    62281 ctcttctcca gtcaggtttg ggaagcctgg tacgtctagg atttcctttt cagggtagag
    62341 aatagcaggt tgtccaccta gctggggaag agagtcgatg atttgaacct tggcttggcg
    62401 taggtgggca taaaaggctg caaaaagccc gacaggacca ccacccacaa tggtaatatc
    62461 atagagttga gacatagttt ctcctttgtt ttttctagtc agtttatttt atcatatttt
    62521 tgttcaatgt aaaatggact aggataggga aaaaaatgcc agaaaatggt ataataggac
    62581 gaaagtgttt tgatagggag gatatcaggg atgaattttc aacaattatc caatctgcaa
    62641 tattggacca gtttgtttgc aagtccatgg acgatagcta tcaatctgat tgatattttg
    62701 attgttgctt atattttata ccattttaca aaagctattg caggaaccaa gattatgatt
    62761 ttggtacgtg gagttttggt gtttatttta gctcaaatcc ttgcaaatat gattggtttg
    62821 actacgattt cttggttaat caatcaaatt attacttatg gggttattgc ggcggttgtt
    62881 atcttctctc cagagattcg gactggtttg gaacgtttgg gaagagcgac agattttttt
    62941 tccaatgccc ctattagtgc tgaggaacag atgattcgtg cctttgttaa gtctgttgaa
    63001 tacatgagtc ctcgtaaaat cggggccttg gttgctattc agcgtgtacg taccttgcag
    63061 gagtatattt cgacaggaat tcccttggat gctaagattt ctgcagaact tctcattaac
    63121 atttttattc ccaacactcc cctacatgat ggtgcggtga ttatcaaaga agaacgtatc
    63181 gctgtgacgt ctgcctatct gcccttgaca aaaaacacag ggatttccaa ggaatttggg
    63241 accagacacc gggcggctat cggtttatca gaagtctcag atgccttgac ttttgtcgta
    63301 tcagaggaaa cgggaggaat ttcgataacc tataatggaa ggtttaagca caacctaaca
    63361 cttgatgaat ttgaaacaga attacgtgaa atcttacttc caaaagagga agtgggtctt
    63421 agttttaaag aacgattgct aggaggatgg aaacatgaaa aaaaatagtt tatatatcat
    63481 atcctcactc ttttttgctt gtgtcttatt tgtctatgct acggcgacga attttcaaaa
    63541 cagtaccagt gctaggcagg taaaaacgga aacctatact aatacagtaa caaatgtccc
    63601 tattgacata cgctataata gtgataagta ttttattagc ggttttgctt cagaagtatc
    63661 agtggtcttg actggggcaa atcgcctatc gctagctagt gaaatgcaag aaagtacacg
    63721 taaattcaag gttactgctg acctaacaga tgccggtgtt ggaacgattg aagttccttt
    63781 gagcattgaa gatttaccca atgggctgac cgctgtagcg actccgcaaa aaattacagt
    63841 caagattggt aagaaggctc agaaggataa ggtaaagatt gtaccagaga ttgaccctag
    63901 tcaaattgat agtcgggtac aaattgaaaa tgtcatggtg tcagataaag aagtgtctat
    63961 tacgagtgac caagagacat tggatagaat tgataagatt atcgctgtct tgccaactag
    64021 cgaacgcata acaggtaatt acagtggttc agtacctttg caggcaatcg accgcaatgg
    64081 tgttgtctta ccggcagtta tcactccgtt tgatacaata atgaaggtga ctacaaaacc
    64141 agtagcacca agttcaagca catcaaattc aagtacaagc agttcatcgg agacatcttc
    64201 gtcaacgaaa gcaactagtt caaaaacgaa ttaaaaatag aaaaggattt tataacaatg
    64261 ggtaaatatt ttgggactga tggagtccgt ggagaagcta acctagaact aacaccagaa
    64321 ttagccttta aactaggacg ttttggaggc tatgttctta gtcaacatga aacggaagcg
    64381 ccgaaagtct ttgtaggacg tgacacacgt atttcagggg aaatgctgga atcggccttg
    64441 gtggcaggtc tcctttcagt agggattcac gtatacaaac ttggtgtcct tgcaacacca
    64501 gcagtagctt acttggttga aactgaagga gcaagtgccg gtgtcatgat ttctgctagc
    64561 cacaacccag cccttgataa cggaatcaag ttctttggcg gtgatggctt caaactagat
    64621 gatgaaaaag aagcagaaat tgaagccttg ctagatgctg aggaagacac tcttcctcgt
    64681 ccaagtgcag aaggcttagg aattttggta gattatccag aaggcttgcg taagtatgaa
    64741 ggataccttg tttcaactgg aactcctctt gatggaatga aggttgcctt ggatacagct
    64801 aatggagcag cttctaccag tgcccgtcaa atctttgcag accttggtgc ccaattgacg
    64861 gttatcgggg aaacaccaga cggtcttaac atcaacctta atgttggttc aacacatcca
    64921 gaagcccttc aagaagtggt caaagaaagt gggtcagcta ttggtttggc ctttgatgga
    64981 gacagtgacc gcttgattgc tgttgatgag aatggtgaca tcgtcgatgg tgacaagatt
    65041 atgtacatca tcggaaaata cctttctgaa aaaggacaat tggctcaaaa tacaattgtg
    65101 acaactgtta tgtctaacct tggtttccac aaggccttga atcgcgaagg tattaacaag
    65161 gcagttactg cagttggtga ccgctacgtt gttgaagaaa tgagaaaatc aggctacaac
    65221 cttggtggtg aacagtctgg tcacgttatc ttgatggatt acaataccac aggtgatggt
    65281 caattatcag cagttcaatt gactaaaatc atgaaggaaa ctggtaagag cttatcagag
    65341 ttggcggcag aagtaacgat ttatccacaa aaattagtta atatccgagt ggaaaacgtc
    65401 atgaaggaaa aggccatgga agtgccagct atcaaggcca tcatcgagaa gatggaagaa
    65461 gaaatggcgg ggaacggccg tatccttgtt cgtccaagtg gaacagagcc cctcttgcgt
    65521 gttatggcag aagcgcctac aacagaagaa gtggactact atgttgatac catcacagat
    65581 gtagttcgtg ctgaaattgg gattgactaa atcctagaaa actagatgaa aataaaaaat
    65641 tatggtacaa tagcttataa tattgtagcc gatggagaaa gacatgaatc ttaaacgtga
    65701 acaagaattt gttagtcagt atcattttga tgcgcgtaat tttgaatggg aaaatgaaaa
    65761 tggagctcct gaaaccaagg tggatgtgaa tttccaattg ctccaacatg accaagaaaa
    65821 tcaagtgact tccttgattg tcatcttgag ctttatgatt gtatttgata agtttgtcat
    65881 tagtggaaca atctcacaag taaaccatat cgatggccgt attgtcaatg aaccaagtga
    65941 attgaaccaa gaagaggtgg aaaccttggc tcgtccatgt ttgaacatgc tcaatcgttt
    66001 gacttacgaa gtgactgaaa ttgcattaga cttaccagga attaatttgg agttctaata
    66061 tgaaattagc tgttttcaca gattcctctg cttatctcag tgaagagacc ttgcaaagag
    66121 aagacttgtt tgtcttggat attcctgtca atattgatgg tgaggaatat gtcgaaggca
    66181 tcaatctgtc tgctgaggaa ttttaccaaa aaatggctca ggcttctgaa ttgcctaaga
    66241 ccagtcaacc aagtattgcc aagttagatg agatcttaac ttcgctcaaa gaacaaggct
    66301 atacacatgc cttggggctt ttcctatctt ctggaatttc aggtttttac caaaatatcc
    66361 agtatatggt cgatgactat gagggcttaa ccattgcttt cccagacact ttgattacaa
    66421 gtgctcccct aggcatcatg gttgaaagcg tctttaattg gcgtgatcag ggcgatgatt
    66481 ttgccagcat tcaggataag ctagccattc aaatcagccg tacgtcagcc tttatcatgg
    66541 tagatgactt ggatcatttg gtaaaaggtg gacgcctttc aaatggggct gccattttgg
    66601 gcaatttgct tagcattaag ccaatccttt attttaacga tcaaggtgtg attgaagttt
    66661 acgaaaaagt tcgtactgaa aagaaggcca ccaaacgctt aattgaaatt atcaaggaaa
    66721 caacggcttc aggtcaatac cgggtcattg tcattcatgg gaatgatcct gaaaaggctg
    66781 aagaattgcg tcagcatttg cttgattttg gcttgggttc ggatgtttca cttgctacat
    66841 ttggtagtgt cattggaacg cacctaggag caggaagtat tgctctgggt tatattccag
    66901 tgatttagta ggcacttcta ggctagttaa tcttagcaat tgaacacgga ctacctgcct
    66961 cttgaaaaaa gatgtctgtc ttgcctttcg tggaaagtca gtgccattcc ctatttttca
    67021 tgggtggcta agatcctttg tattttgata attgaacacg cctggaaccc tgtgtgaaaa
    67081 agatagttct tccaaggagt agacacttct tgatcagaat tcctattttc actttgtgtt
    67141 cttacaggct ttgtatctta gacaggagta gagatgagta ttcgagtaat tattgccggt
    67201 tttaagggaa agatgggtca agctgcttgc cagatggtct tgcctgattc agatttggac
    67261 ttggtagcag ttttggatcc ttttgagtct gagtcagaat ggcagggtat tcctgttttc
    67321 aaggataagg ctgatttagc tggttttgaa gcggatgtct gggtagactt tactactcca
    67381 gctgttgcct acgaaaatac acgttttgct cttgaaaatg gctttgctcc agtagttgga
    67441 acgactggtt tcacgagtga agaaattgca gagttaaaag aattttctcg tgcccaagac
    67501 ttgggtggcc tgattgcccc taactttgcc ttgggtgctg tcttactcat gcaatttgcg
    67561 acgcaggctg ccaaatattt cccaaatgtg gagattattg agctccatca tgacaagaaa
    67621 aaggatgctc cgagtggaac agccattaaa acagctgagt tgatggcaga ggttcgagag
    67681 tccattcagc aaggcgcagc agatgaggaa gagctgattg ctggtgctcg tggtgctgac
    67741 tttgatggta tgcgcatcca ctcagttcgt ttgccaggtt tggtagccca tcaggaagtc
    67801 atctttggca atcaaggaga aggattgacc ctccgtcatg actcctatga tcgcatctcc
    67861 ttcatgacag gagtcaattt gggaattaaa gaagttgtca agcgtcatga gcttgtctat
    67921 ggattagaac acttattatg agattaacgc aaatgccttc tgaatttcag aaggctttac
    67981 cagtattaga aaaaattaaa gaagcaggct ttgaggctta ttttgttggg ggctctgttc
    68041 gagatgccct tctccatagc cctatccacg atgtggatat tgcgacgtct tcttatccag
    68101 aagagaccaa gcagattttt ccgcgaacag ccgatatcgg aatcgagcat ggaaccgtct
    68161 tggtcttaga tggggatgag gagtatgagg taacaacctt tcggacagag gatgtctatg
    68221 tggactatcg cagacccagt gcggtttcct ttgtgcgctc gctagaagaa gacctcaaac
    68281 gccgtgattt cacagtcaac gcctttgcct tggatgagac aggagaaatc gttgacttgt
    68341 tccatggttt agaagatttg gaaaagcaag tcttgcgagc agttggagtg gctagtgagc
    68401 gtttcaacga agatgcttta cggattatgc gtggtttccg ttttcaggct agtcttggtt
    68461 ttgcacttga gccagaaaca tttaaagcta tgaagacctt gacgccgctt ttggagaaca
    68521 tttctgtaga gcgtaccttc gtcgagtttg ataaactctt gctggctcca ttttggagaa
    68581 ggggcttggc ttccatgatt gagagtcaag cttatgacta tctccctgat atggcatcta
    68641 gccagaacaa gctcaacaga ctgtttgatt tggagactga ttttactttt gaatcctctg
    68701 aacaagcctg ggcggcttta ctatgggctt tggagattga aaatgcgcag tcatttttga
    68761 aatcttggaa gacctcacgc cagtttgcca agcaagttca ggatttgctg attattttgg
    68821 ctctgcgtga aaatggagaa ttgagcaagc gagattgtta tcgctttgac atagatttgc
    68881 ttttacaggc tgaaaatctt cgtcaagctc gaggaaaaga agtcaaccca caagccatca
    68941 cagaaaaata ccaaagcttg actatccatg acaagaaaga gattcagatt aatggcgata
    69001 ttttgatcaa ggaatatggc tatcagccag gcccagactt gggagagatt ttaacagaga
    69061 ttgagtttgc cattgtcgat ggagaattgg agaataatcg tgaagccatc catgcttacc
    69121 tgagggagaa aaaatgagtg attttatcgt tgaaaaacta agtaaatccg ttggtgacaa
    69181 gaccgttttt agggatattt cctttattat ccatgactta gacagaattg gtttaatcgg
    69241 tgtcaatggg actggcaaga ccaccctttt ggacgtcctt tctggtgttt ctggctttga
    69301 tggggatgtc agtccttttt cagctaaaaa tgattaccag attggttact tgactcagga
    69361 tcctgatttt gatgatagaa agacagtttt ggatacggtt ctatctagtg aactcaagga
    69421 aatccagctc attcgtgagt atgaattgat tatgctcgac tatagtgagg acaagcaggc
    69481 gcgtttggaa cgtgtcatgg cagagatgga ctctctccaa gcttgggaaa tcgagagtca
    69541 ggtcaagacc gttcttagca aattgggcat tcaagactta tctactcctg ttggggaatt
    69601 gtcaggtggt ctgagaagac gggtacagtt ggcacaagtc ttacttggca accacgacct
    69661 cttgcttttg gatgagccga ccaaccatct ggatattgcg attattgagt ggctgaccct
    69721 ctttttgaaa aattctaaga agaccgtcct ttttatcact cacgatcgtt atttcttaga
    69781 cgctttgtca acacggattt tcgagttgga gcgagcaggc ttgacagaat atcagggcaa
    69841 ttaccaggac tatgttcgcc ttaaggcaga acaggacgag cgcgatgcaa ctctacttca
    69901 taaaaaagaa caactctaca aacaagaact ggcctggatg cgcagacaac cgcaggcacg
    69961 tgcgaccaag caacaagctc gtatcaatcg tttccatgat ctgaaaaagg aagtttcagg
    70021 cagtagtgct gagacagact tgactatgaa ctttgaaacc agtcggattg ggaagaaagt
    70081 catcgagttt caggatgttt cctttgccta tgaaaataag cccattttgc aagattttaa
    70141 tctcttggta caggtcaaag atcgtatcgg aatcgttggg gacaatggtg ttggaaaatc
    70201 aaccctactt aacctgattg caggaagtct tgagccgata gcaggacaag ttgtgattgg
    70261 ggaaactgtt cgcatcgcct atttctctca acaaattgag ggtttggatg aaagcaagcg
    70321 tgtgatcaat tacctgcagg aagtggcaga ggaggtcaag accagtggtg gttctacgac
    70381 ttccatcgct gagttgctgg agcaattcct cttcccacgt tcgacgcatg ggaccttgat
    70441 tgagaaatta tctggtggag agaaaaaacg tctttatctc ctcaaactgc tcttggagaa
    70501 accaaatgtt cttcttttag acgagccaac caatgacttg gatattgcaa ctttgacagt
    70561 cttagagaat ttcttgcaag gttttgcagg tcccgtttta acagtcagtc acgaccgcta
    70621 tttcttggat aaggtagcga ccaagattct cgcttttgag gatggcaaga ttcgtccttt
    70681 ctttggtcat tacaccgact atcttgatga aaaagctttt gaaacagata tggccaatca
    70741 agtgcaaaag gccgaaaagg aaaaagtggt caaggttcga gaagacaaga aacgcatgac
    70801 ctaccaagaa aagcaggagt gggcaagtat tgaaggtgat attgaaacct tggaaaaacg
    70861 tatcgctgct attgaagagg aaatgcaggc taacggctct gactttggta agctggctac
    70921 tctccaaaaa gaattggatg agaaaaatga agcactcctt gaaaaatacg aacgctatga
    70981 gtatctcagt gaatttgata gttaagacta ttcgaaaatc aaagtgcaaa ctatgtcggc
    71041 ttcacattgc cgactcaagt acagcctgtg accggcttcc taatttgcac tttgattttt
    71101 attgagtata aaagaataga aaaatcccgt ctcatgaaag gatttctcta ttcttttttg
    71161 gtttcttgat gaaagatatt ttgccaggtt tcgtggcgac gccagatact ggtatggatg
    71221 ataccatcta actcatagca gatgagtttg gttttttgac tgatggaagt aatttgaatg
    71281 tccttgatag cagaatttaa ctcttttttg gtcttataag cctctttatc catctgctct
    71341 ccatcttggc gaatatagac aaaatcatca gtcaagagtt cttctagttg gtttccttgg
    71401 tcaatcaatt gttcacgcat aagcaatttt ttatagacat tgtctaaaat ttcatcctca
    71461 gggataggtg ctggttcgat atggacatcg gtatcaaaga cgccaaaacg ctcctccagc
    71521 atagactcga cctgatccgc gatttcatgg ctttcaaaaa cagacaagtc aggattcatc
    71581 tctagtgtaa tatccaggta gatgttgcta ccgtaggtgc gacctctttg cgatttgacc
    71641 ttgctgattt tgggaatttc catgatagcc ttttggtagt cctcgagcag gcggtcgtca
    71701 aagccatctg aaagactaaa ggaagactcg atgaagatat cataggcagt cttcaagata
    71761 aagaaagtga tgatgatagc aaccagttta tccacaatcg gataattgaa actactagct
    71821 aggatggcaa tggcggttcc aagtgaggta acagcgtcag aaagattgtc cttagcagct
    71881 gccttcagcg ccttggagtt ggatttctta ctgaggcgag tattgtagag atagaccaca
    71941 aacataatcg ctgcagaaat gattcctaga gttgcaccaa gaggatcaat gaccgtttct
    72001 tcccgactga gaatcttttg aatggtatct cttagaatat cgaaaccgac atagaacatg
    72061 atgatagaag tgatcaagct tgccaaatct tcaatcttcc aatgaccaaa acggtggtca
    72121 cggtctgcag gctggcgcgc catccgaatc ccgattaaga gggccacatt tccaatgatg
    72181 tccgatacgt tattaaaacc atcggccacc aaactggatg aatgaaggag atgaccagct
    72241 gctaatttag ctgcagacaa gatcaaatag gtcgaaatac tgataatggc tccacgctca
    72301 gctaacttga gatttgagat agattgcttc attcaacttc ctttctagtc acatagcatt
    72361 ctaccattat actggttttc aaggaaaaat tcaaataagg cagtttttac tctttgaaaa
    72421 ctccccaaaa tttggtataa tggtactact caagggagta gctggcagaa acctgtgata
    72481 gtgtcgtcat tccgaatttt atgctgaaaa gtatgctttc cggccctatc ttaaacagcg
    72541 agacttgtta tgattaacag gtctcttttt gtttgtcgtg aaaagatatg acagaaagag
    72601 agtctgtttt gcacacaatg tcaaggagga gacacatgtc aaaagaacaa aaacgccaag
    72661 cgttttatac tcagagccct gaagaggtct tgcaggctgt ggatgcgacc gagcaaggtt
    72721 tgtcatcaag tgaggcggaa aagcgccttg ccgaatttgg gcacaatgaa ctcgaagaag
    72781 gcgagaaacg atcaatcttg gtcaaattca tcgagcaatt taaggatttg atgattatca
    72841 tcctagttgc ggcagcaatc ttgtcagtcg tgacttctgg tggggaagat atcgcagatg
    72901 ccattatcat cctagctgtg gttatcatca acgctgcctt tggtgtttac caagaaggaa
    72961 aagctgaaga agctatcgaa gccctcaaat ccatgtctag tccagttgcc cgcgttcttc
    73021 gtgatggaca catggcagag attgactcta aagaattggt accaggcgat atcgttgccc
    73081 ttgaagcagg tgacgtggta ccagcggacc tacgtttgct agaagccaac tctcttaaaa
    73141 ttgaagaagc agccttgacg ggtgaatctg taccagtcga aaaagacttg tcagtcgagc
    73201 ttgcgacaga tgctggtatt ggtgaccgtg tcaacatggc cttccaaaac tcaaacgtaa
    73261 cctatggtcg tgggatgggt gttgttgtca atacaggtat gtacactgaa gttggtcata
    73321 ttgctggtat gcttcaagat gcggatgaga ctgatacacc actcaaacaa aacttgaaca
    73381 acctttctaa ggtcttgacc tatgctatct tggtcattgc ccttattact tttgtagtgg
    73441 gtgtcttcat tcaagggaaa aatccacttg gtgagttgtt gacttctgtt gcccttgccg
    73501 ttgcagccat tccagaagga cttcctgcta tcgttaccat cgttctttct cttggtactc
    73561 aagttttggc caaacgacat tccatcgttc gtaagttgcc agcagttgaa acacttggtt
    73621 caactgaaat catcgcttct gataagactg gtacgctgac tatgaacaag atgacagtcg
    73681 aaaaagtctt ctacgatgcg gttctacatg actcagctga tgatattgaa ctaggtcttg
    73741 aaatgccact acttcgttca gttgtcttgg ccaatgatac gaaaatcgat gtggaaggta
    73801 acttgattgg tgacccaacc gaaacagcct ttatccaata tgccttggac aagggctatg
    73861 atgtcaaagg tttcttagag aaatatcctc gtgtagctga attgccattt gactctgacc
    73921 gtaagctcat gtcaacagtt cacccattgc cagatggtcg tttccttgta gcagtcaagg
    73981 gtgcgccaga ccaactctta aaacgttgtc ttcttcgtga taaggctggg gatattgctc
    74041 cgattgatga gaaggttaca aatctcattc atacaaacaa ttctgaaatg gctcatcaag
    74101 ccttgcgtgt ccttgcaggt gcttataaga ttatcgatag tattccagaa aatctcactt
    74161 ctgaagagct tgaaaatgat ttaattttta ctggtttgat tgggatgatt gaccctgaac
    74221 gtcctgaagc cgctgaggct gttcgtgtgg ctaaggaagc gggaatccgt ccgattatga
    74281 ttacaggtga ccaccaagac acagcggaag ccattgccaa acgtttggga atcattgacg
    74341 caaacgatac agaaggtcac gttttaactg gtgctgaact caatgaactg tcagatgaag
    74401 aatttgaaaa agtcgttggt caatactctg tttatgcccg tgtgtctcca gaacacaagg
    74461 ttcgtatcgt caaggcttgg caaaaacaag gtaaagtcgt tgccatgaca ggtgacggtg
    74521 tcaatgacgc gccagctctg aaaacagccg atatcggtat cggtatggga atcactggta
    74581 cagaggtttc taagggggct tctgatatga ttcttgcaga tgataacttt gcgactatta
    74641 tcgtcgcagt ggaagaagga cgtaaggtct tctcaaacat tcaaaagact attcagtacc
    74701 tactttctgc taatactgct gaagtattaa ccatcttcct atcaaccttg tttggttggg
    74761 atgtcttaca gccggttcat cttttgtgga tcaacttggt aacggatacc ttcccagcta
    74821 tcgctcttgg tgttgaacct gcggaacctg gtgtcatgaa tcataaacca cgtggacgca
    74881 aggcaagctt cttctcaggt ggtgttttga gttctatcat ttatcaaggt gtactccaag
    74941 cagctcttgt tatgagtgtt tatggccttg cgattgctta cccagttcat gtgggtgaca
    75001 atcatgctat tcatgcagat gccctaacga tggcctttgc aacccttggt ttgattcagc
    75061 tcttccatgc ctacaatgtc aagtctgtct accaatccat cttgacagtt ggcccattca
    75121 agtctaagac ctttaactgg tccatcttgg tatcctttat ccttctcatg gcaacaatcg
    75181 ttgtagaacc gcttgaagga atcttccacg taaccaaact agacttgtca caatggggaa
    75241 ttgttatggc tggaagcttc tcaatgatta tcatcgtcga aatcgttaag tttattcaac
    75301 gcaaactcgg ttttgacaag aatgcgattt aagggaggtt tcggatgtca gcatatcaat
    75361 taccgaccgt atggcaggat gaagctagta atcaaggagc ttttacgggg ctaaacagac
    75421 caacagcagg tgcccgtttc gaacaaaact tgccaaaagg agaacaagct tttcagcttt
    75481 attcactggg aacaccaaat ggtgtgaagg ttactatctt attggaagaa ttactagaag
    75541 ctggttttaa ggaagcggct tacgacttgt ataagattgc tatcatggat ggggatcaat
    75601 tcggctcaga ctttgtgaag ctcaatccaa attccaagat tccagcctta ttggaccagt
    75661 caggtactga aaacgtaaga gtctttgagt ctgctcatat tcttctttac cttgctgaga
    75721 aatttggagc ctttttacca agtaatcctg tggaaaaggt agaagttttg aattggctat
    75781 tctggcaagc aggtgcagca ccttttctag gtgggggatt tggacatttc ttcaattatg
    75841 ctcctgaaaa attggaatat cctattaacc gttttacgat ggaagtgaaa cgccagttgg
    75901 atttattgga taaggaattg gctcagaaac cttatattgc aggcaatgac tatacgattg
    75961 cagatattgc tatctggtct tggtatggac agttagttca aggaaatctt taccaaggtt
    76021 ctgcaaaatt cttggatgcc tcaagttatc aaaatctagt aaaatgggca gaaaaaattg
    76081 ccaatcgtcc agctgttaag cgtggcttgg aagtaactta tacagaaatt aaatagcaaa
    76141 aaagtcatct tcggatggct ttttgctaca gttgagggaa agggtttgca gttatcggct
    76201 tttgtgctat aattgctata atatagtaaa aagcaagagg agtgtgagga agtggaaaag
    76261 aaaattgtga aggatatctt atttttatct caagtgtctc agccggcaag tcaggaggac
    76321 ctttatcttg ccagagattt gcaggataca ctcttagcaa atcgtgatac ctgtgttggt
    76381 ctagctgcca atatgattgg ggtgcagaag cgcgtgatta tctttaatct tggcttggtt
    76441 cccgtggtca tgtttaaccc agtgcttctg tcctttgaag gatcctatga ggcagaagaa
    76501 ggctgtttgt ccttggtagg tgtgagatca actaagcgtt atgaaaccac aaggcttgcc
    76561 tatcgtgaca gcaagtggca ggaacagacc attaccttga caggcttccc agctcagatt
    76621 tgccagcatg agctggatca cttggaagga cgaatcattt aggaggaaag caaatgaaac
    76681 gaatagtctt tgaacttatt tttatcgcaa cgacctggta tatcttttta ccgcccctta
    76741 acctgaccag ctgggaattt ctcttcttcc tctgtgggca tttgttagtt gtggcaatct
    76801 tatttggctt tggcaagggg ataaaccttg tcaaaacggt tcatgtgcgc cacggtaagg
    76861 cggaagctgc cttaaatctt gagggtttca aaatcaatcg gttagggaaa attctgttag
    76921 cttcgattgg aggaattctt ctcttggcag ctttggtttc cttggtaact tccagcatgt
    76981 ttcaggctaa aaattatgcc aatgtagtca cggttacgga aaaagacttt actgaatttc
    77041 ctaggagtga caccagtaag gttcctatcc tagatagaag tactgctgaa aaaattggag
    77101 accgctactt gggttcccta accgataagg tgtcgcaata cgtagcggca gatacctata
    77161 cccaattgac aattgatggg aaaccttatc gggtcacacc actagaatat gcagacccta
    77221 tcaaatggtt taacaatcaa gccaagggaa tcggtgagta tatcaaggtg gacatggtaa
    77281 ctggaaatgc ggatttggtg gacttgaaga caccaatcaa gtattcagac tcggagtatt
    77341 ttaaccgtga tgtcaaacgt cacctgcgct tgaagtaccc gactaaaatc tttaaaactc
    77401 catcttttga ggtggacgat gagggcaatc ctttctatgt agcaacggtt taccaaaagc
    77461 aatttggact tgcggttcct cgtcctgctt cagtcattat cttggatgct acaaatggag
    77521 aaaccaagga atacagctta tcagatgttc cagaatgggt ggacaggatc tatccagcag
    77581 aggaaaccat tgagcaaatc aactacaacg gcaagtacaa ggacggtttc ttgaatgcca
    77641 tgatttccaa gaaaaacgtg acccagacta ccaatggcta taattacttg tctatcggta
    77701 atgacatcta tctctacaca ggtgtgacgt cggctaatgc ggatgagagt aatcttggtt
    77761 tcatccttga aaatatgcga acaggagaaa ttactaagta tagcttggct tctgcgacag
    77821 aagaatcagc ccgtgaatca gcagaaggtg ctgttcagga gaaatcctac aaagcaacct
    77881 tcccaatcct catcaacctc aatgacaagc ctctctacat catgggctta aaggacaatg
    77941 ctggcttggt caaagagtac gccctggtag acgcagtcga gtaccaaaat gttatcgttg
    78001 ctactacagt ggaagagatg ctcagcaagt atgccaataa aaacgacctt gaaattgaca
    78061 atgcaacgac agaaagcatc aagggagtag tagcagacct caaatcagct gttatcaagg
    78121 gagacactgt ctacttcttt aaagttgatg gcaagatata caaggtaaag gcttcagtat
    78181 ccgatgacct tccttacctt gaaaatggta aaaccttcga aggtcaagta ggaaaagaca
    78241 attatctcaa gacctttaaa gtacaataat actcttcgaa aatctctagc tctatctgca
    78301 acctcaaaac agtgttttga gcagcc
//
LOCUS       DAVKLW010000002        61822 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKLW010000002 DAVKLW010000000
VERSION     DAVKLW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.002"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(105..1466)
                     /gene="dnaA"
                     /locus_tag="VW324_000083"
     CDS             complement(105..1466)
                     /gene="dnaA"
                     /locus_tag="VW324_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9725793.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(1679..2437)
                     /locus_tag="VW324_000084"
     CDS             complement(1679..2437)
                     /locus_tag="VW324_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9725794.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(2495..3676)
                     /locus_tag="VW324_000085"
     CDS             complement(2495..3676)
                     /locus_tag="VW324_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9725795.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            3859..4338
                     /gene="rlmH"
                     /locus_tag="VW324_000086"
     CDS             3859..4338
                     /gene="rlmH"
                     /locus_tag="VW324_000086"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9725796.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4382..4455
                     /locus_tag="VW324_000087"
     tRNA            4382..4455
                     /locus_tag="VW324_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4416..4418,aa:Arg,seq:tct)
     gene            4621..4746
                     /gene="comC"
                     /locus_tag="VW324_000088"
     CDS             4621..4746
                     /gene="comC"
                     /locus_tag="VW324_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9725797.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            4767..6092
                     /locus_tag="VW324_000089"
     CDS             4767..6092
                     /locus_tag="VW324_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9725798.1"
                     /translation="MDLFGFGTVIVHLLIISHNYHLICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIAIFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFS
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            6089..6841
                     /locus_tag="VW324_000090"
     CDS             6089..6841
                     /locus_tag="VW324_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9725799.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQFIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            6893..6964
                     /locus_tag="VW324_000091"
     tRNA            6893..6964
                     /locus_tag="VW324_000091"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6926..6928,aa:Glu,seq:ttc)
     gene            6970..7043
                     /locus_tag="VW324_000092"
     tRNA            6970..7043
                     /locus_tag="VW324_000092"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7004..7006,aa:Asn,seq:gtt)
     gene            complement(7084..7626)
                     /locus_tag="VW324_000093"
     CDS             complement(7084..7626)
                     /locus_tag="VW324_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9725800.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            7805..8572
                     /locus_tag="VW324_000094"
     CDS             7805..8572
                     /locus_tag="VW324_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9725801.1"
                     /translation="MIGISLIPALYNIIFLSSMWEPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            8576..8995
                     /locus_tag="VW324_000095"
                     /pseudo
     CDS             8576..8995
                     /locus_tag="VW324_000095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9023..9337
                     /locus_tag="VW324_000096"
     CDS             9023..9337
                     /locus_tag="VW324_000096"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9725802.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(9438..11990)
                     /locus_tag="VW324_000097"
     CDS             complement(9438..11990)
                     /locus_tag="VW324_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9725803.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLNRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(12052..13674)
                     /locus_tag="VW324_000098"
     CDS             complement(12052..13674)
                     /locus_tag="VW324_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9725804.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEIQAKVKELWKD"
     gene            13880..14905
                     /gene="trpS"
                     /locus_tag="VW324_000099"
     CDS             13880..14905
                     /gene="trpS"
                     /locus_tag="VW324_000099"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9725805.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            15057..16535
                     /gene="guaB"
                     /locus_tag="VW324_000100"
     CDS             15057..16535
                     /gene="guaB"
                     /locus_tag="VW324_000100"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9725806.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(16586..17683)
                     /gene="recF"
                     /locus_tag="VW324_000101"
     CDS             complement(16586..17683)
                     /gene="recF"
                     /locus_tag="VW324_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9725807.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(17686..18054)
                     /gene="yaaA"
                     /locus_tag="VW324_000102"
     CDS             complement(17686..18054)
                     /gene="yaaA"
                     /locus_tag="VW324_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9725808.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            18213..19463
                     /locus_tag="VW324_000103"
     CDS             18213..19463
                     /locus_tag="VW324_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9725809.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            19460..20743
                     /locus_tag="VW324_000104"
     CDS             19460..20743
                     /locus_tag="VW324_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9725810.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            20785..21606
                     /locus_tag="VW324_000105"
     CDS             20785..21606
                     /locus_tag="VW324_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9725811.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            21617..22162
                     /gene="pgsA"
                     /locus_tag="VW324_000106"
     CDS             21617..22162
                     /gene="pgsA"
                     /locus_tag="VW324_000106"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9725812.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            22159..22986
                     /locus_tag="VW324_000107"
     CDS             22159..22986
                     /locus_tag="VW324_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9725813.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            22971..23810
                     /locus_tag="VW324_000108"
     CDS             22971..23810
                     /locus_tag="VW324_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9725814.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            23803..24597
                     /locus_tag="VW324_000109"
     CDS             23803..24597
                     /locus_tag="VW324_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9725815.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            24656..25474
                     /gene="mreC"
                     /locus_tag="VW324_000110"
     CDS             24656..25474
                     /gene="mreC"
                     /locus_tag="VW324_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9725816.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            25474..25968
                     /gene="mreD"
                     /locus_tag="VW324_000111"
     CDS             25474..25968
                     /gene="mreD"
                     /locus_tag="VW324_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9725817.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            26062..27240
                     /locus_tag="VW324_000112"
     CDS             26062..27240
                     /locus_tag="VW324_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9725818.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            27464..28243
                     /gene="rpsB"
                     /locus_tag="VW324_000113"
     CDS             27464..28243
                     /gene="rpsB"
                     /locus_tag="VW324_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9725819.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            28322..29362
                     /locus_tag="VW324_000114"
     CDS             28322..29362
                     /locus_tag="VW324_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9725820.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            29609..29932
                     /gene="tnpB"
                     /locus_tag="VW324_000115"
     CDS             29609..29932
                     /gene="tnpB"
                     /locus_tag="VW324_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9725821.1"
                     /translation="MIIQLSDLGQVHLVCSKIDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENVRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(30087..30395)
                     /locus_tag="VW324_000116"
                     /pseudo
     CDS             complement(30087..30395)
                     /locus_tag="VW324_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30386..30784
                     /locus_tag="VW324_000117"
     CDS             30386..30784
                     /locus_tag="VW324_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9725822.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(30981..31901)
                     /gene="cysK"
                     /locus_tag="VW324_000118"
     CDS             complement(30981..31901)
                     /gene="cysK"
                     /locus_tag="VW324_000118"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9725823.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(32186..32821)
                     /locus_tag="VW324_000119"
     CDS             complement(32186..32821)
                     /locus_tag="VW324_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9725824.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            32877..34175
                     /locus_tag="VW324_000120"
     CDS             32877..34175
                     /locus_tag="VW324_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9725825.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILAEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            34172..34834
                     /locus_tag="VW324_000121"
     CDS             34172..34834
                     /locus_tag="VW324_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9725826.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            34914..35462
                     /gene="raiA"
                     /locus_tag="VW324_000122"
     CDS             34914..35462
                     /gene="raiA"
                     /locus_tag="VW324_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9725827.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            35598..37571
                     /locus_tag="VW324_000123"
     CDS             35598..37571
                     /locus_tag="VW324_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9725828.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            37568..38020
                     /gene="rplI"
                     /locus_tag="VW324_000124"
     CDS             37568..38020
                     /gene="rplI"
                     /locus_tag="VW324_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9725829.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            38064..39416
                     /gene="dnaB"
                     /locus_tag="VW324_000125"
     CDS             38064..39416
                     /gene="dnaB"
                     /locus_tag="VW324_000125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9725830.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            39418..39684
                     /locus_tag="VW324_000126"
     CDS             39418..39684
                     /locus_tag="VW324_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725831.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            39773..41119
                     /gene="cbpD"
                     /locus_tag="VW324_000127"
     CDS             39773..41119
                     /gene="cbpD"
                     /locus_tag="VW324_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9725832.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      41202..41304
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41375..41665
                     /locus_tag="VW324_000128"
     CDS             41375..41665
                     /locus_tag="VW324_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9725833.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETPH"
     gene            41628..42386
                     /locus_tag="VW324_000129"
     CDS             41628..42386
                     /locus_tag="VW324_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9725834.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            42424..43431
                     /locus_tag="VW324_000130"
     CDS             42424..43431
                     /locus_tag="VW324_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9725835.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            43431..44159
                     /locus_tag="VW324_000131"
     CDS             43431..44159
                     /locus_tag="VW324_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9725836.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            44278..44736
                     /locus_tag="VW324_000132"
     CDS             44278..44736
                     /locus_tag="VW324_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9725837.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            44738..47170
                     /locus_tag="VW324_000133"
     CDS             44738..47170
                     /locus_tag="VW324_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9725838.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            47491..48144
                     /gene="rr06"
                     /locus_tag="VW324_000134"
     CDS             47491..48144
                     /gene="rr06"
                     /locus_tag="VW324_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9725839.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            48141..49472
                     /gene="hk06"
                     /locus_tag="VW324_000135"
     CDS             48141..49472
                     /gene="hk06"
                     /locus_tag="VW324_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9725840.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            49555..50082
                     /locus_tag="VW324_000136"
     CDS             49555..50082
                     /locus_tag="VW324_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9725841.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            50161..52323
                     /gene="pspC"
                     /locus_tag="VW324_000137"
     CDS             50161..52323
                     /gene="pspC"
                     /locus_tag="VW324_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9725842.1"
                     /translation="MFASKSERKVHYSIRKFSVGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGQDISKKYADEVKSHLEKILSEIQLDKRKHTQNLAFNKKLSRIQTEYFYLK
                     KKLKAELTSKTEELTSKTKKELDAAFEQFKKDTLSTEPEKKVAEAEKKVAEAKKKAED
                     QKEEDRRNYPTNTYKTLELEIAEFDVKVKEAELELLKEKAKESRDEEKIKQAEAEVES
                     KQAEATRLENIKTDRKKAEEEAKRKADAKLKEANVATSDQGKPKGRAKRGVPGELATP
                     DKKENDAKSSDSSVGEETLPSSSLKSGKKVAEAEKKVEEAEKKAKDQKEEDRRNYPTN
                     TYKTLELEIAESDVKVKEAELELVKEEAKEPRDEEKIKQAKAEVESKQAEATRLEKIK
                     TDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPKPEKPAPAPK
                     PENPAEQPQPAPAPQPEKPAPKPEKPAPAPKPENPAEQPKAEKPAQPSTPKTGWKQEN
                     GMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQ
                     NNGSWYYLNANGSMATGWLQNNGSWYYLNANGDMATGWLQNNGSWYYLNANGDMATGW
                     LQNNGSWYYLNSNGAMVTGWLQNNGSWYYLNANGDMATGWVKDGDTWYYLEASGAMKA
                     SQWFKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWVN"
     gene            complement(52553..53533)
                     /gene="dusB"
                     /locus_tag="VW324_000138"
     CDS             complement(52553..53533)
                     /gene="dusB"
                     /locus_tag="VW324_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9725843.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(53520..54392)
                     /gene="hslO"
                     /locus_tag="VW324_000139"
     CDS             complement(53520..54392)
                     /gene="hslO"
                     /locus_tag="VW324_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9725844.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            54557..55984
                     /locus_tag="VW324_000140"
     CDS             54557..55984
                     /locus_tag="VW324_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9725845.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            56143..57651
                     /gene="glpK"
                     /locus_tag="VW324_000141"
     CDS             56143..57651
                     /gene="glpK"
                     /locus_tag="VW324_000141"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9725846.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            57693..59519
                     /gene="glpO"
                     /locus_tag="VW324_000142"
     CDS             57693..59519
                     /gene="glpO"
                     /locus_tag="VW324_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9725847.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESNWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            59587..60291
                     /locus_tag="VW324_000143"
     CDS             59587..60291
                     /locus_tag="VW324_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9725848.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            60588..60791
                     /locus_tag="VW324_000144"
     CDS             60588..60791
                     /locus_tag="VW324_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725849.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(60878..61486)
                     /locus_tag="VW324_000145"
     CDS             complement(60878..61486)
                     /locus_tag="VW324_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725850.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT        19316 a        10425 c        13179 g        18902 t
ORIGIN      
        1 tgtgaaatct gtggaaaaca gcctttaaac aagtcatatc aagtttttta gcttgtttaa
       61 aaaatgtgga taaaaaagat aaaaaactaa aagttatcca caaattattt gattttcttt
      121 ttgattgatt caatttctaa acgtaaatta tcgtcttgat caatcaaaga ttttattttg
      181 gcatgggcat gaatgactgt ggtatgatct tttcccccaa attccttccc aatttttgga
      241 agactattat ctgttagttc tctagataaa tacatggcta cttgacgggc caaaacaata
      301 ttttgaaggc gtctacttcc cttcatttct ttgatactaa caccataaaa gttaccaact
      361 tcagtttgga ttttatcaat tgggatgacg agcatttggc taacatcttg tttgcgggct
      421 ctaatggctt ctgcagcaat atcaatagtg atatccttga ttttttttac tctggcaatt
      481 aaagtgatgt cgttgatggc tccctcaaga tctcgaacat ttgaatcaaa ttgcccagct
      541 aggtattcta gagtatcact ttggaaattg tagcctaaat gttccgtttt actttgtaaa
      601 atggcaatac gtgtttcaaa gtcagggggt gtgatagttt gtgtcaatcc ccaactaaaa
      661 cgcgtgacaa gcctctcctc gagcccttct agatgttttg gactacgatc actcgttagg
      721 acaatctgtt tttgcttgtc atgaagggcg ttaaaggtat tgaaaaattc ttcctgagtt
      781 gcgacttttt ttccgctgag tgactgtata tcatcgatta acaaaagatc aagactacga
      841 taggtctttt taaacttttc catttcccca agtcttaggt gatcaagaaa gtcattaata
      901 aagctttcgg cagggatata tttaacacgc gcattaggaa tattttttag aatttcattt
      961 ccaatagcgt ttaataagtg agtcttacca aggcctggtc ctccatagat aaaaagaggg
     1021 ttataggtca gagccaaatc ttcagagaca gctaaagcgg ctgatacagc ccaaacattt
     1081 ccatcccctt gaataaagtt atcaaaggta tacttttctt ttaatcccgt atctgaataa
     1141 ggaatagata ctaactttgg actatagtca taaagagtta aatttgtagc ttcttcaact
     1201 tgtgagctag tcgtatcttg aggtttggtg aaaatatagt ggggagttat ttcagcgtca
     1261 taaatttcaa aaccagctac tacaataata tcttttagtt gtttttccca gaccatttcc
     1321 atttcagagc gaggtagaaa tatagtggca acattttcct ctaccttgat gagttcagct
     1381 tgaatagcat agaaatcata catggatcga gtcagtcttt cttgtgcaaa ttctaatata
     1441 cgattccaaa attgtttttc tttcaatcct ttctcctcct ttactattta aaagtttaat
     1501 actagagata ttttaccata gatagcaaga attttccaca agctgtgaaa aataatccac
     1561 aatgttacta aactttatcc acaggttggg gataaaagaa gaaattattg atttattaag
     1621 ctttttatcg aaaaacatag tggataacct tgtgagataa aaaaataaaa gaacagcctt
     1681 atttcaggct gttgataatt ctactatatt cttcttgatt tgaaaaagaa ataatgattt
     1741 ttccactgtc ttttttagat agtttaattt ctacatctaa tccgagtagt tttcttaact
     1801 gtttttcttc attttgtatg aaatgattag ttttttgctg tttcttttgt tttttctctg
     1861 tcagaagagc ttctaacttc cttacagaaa tatcttcttc tataatccgt tgaaagaaat
     1921 agtcttgttg ttccttattt aacccaacta gggaacgcgc atgggcttgt gatagtttgc
     1981 cattttctac ttctgagaga atctgttctg gcaaggaaag taaacgaatg gagttgctga
     2041 tatatggacg agacttgccc atcttatctg caatttcagc atgggtgaat cctttctcta
     2101 cgagagattc ataggcgcgt gcttcttcta ttgggtttaa attttctctc tgtaaatttt
     2161 caataatgga ctggaccatc atctcttggt ctgaaatctg tttaacaaca gctgggatag
     2221 accgtagacc agctaaaagt gaagcccgat agcgtctctc tcctgcaagg atttcataac
     2281 caataacagg agattgacga acaataatcg gttgaatgac cccattttct ttgatagact
     2341 gtgctagttc atctagtttt tctctatcaa attcttttcg gggttgatag ggattttttt
     2401 gtatatctgt gatagaaatc atttcaaatt tttccatgat tctacactaa cacatctttt
     2461 ctcttatgta aagctttctt tacatagatg tcaattaaga ttctaaatca cctgaactct
     2521 tgttaagttt gatagaggta gtttcttctt tcccgttacg atagtaggtt atcttaatgg
     2581 tgtctccgat agaatggttg taaagagcac tttgtaaatc tgttgatgaa gcaatctctt
     2641 tgtcatctac ttttgtaatt acatcgtatt tttcaaggtg accattggca ggcatattac
     2701 tttgtaccga acgaacaact acaccagatg taacattact tggaatattg agtcttctga
     2761 tgtcgcttgt actcacatta gataaattaa ccatctggat tcccaaagct ggacgagtca
     2821 cttttccgtt tttttctaac tgttcaataa tattgatagc atcatttgca ggaattgcga
     2881 aaccaagacc ttctacagat gttcctccat ttgtagcaat tttacttgag gtaattccga
     2941 taacctgccc ttgaatattg atcagtgggc cgccagagtt acctgggtta atagcagtat
     3001 cagtttggat ggcttttgta gaaatagctt gtccatcttc cgatcttaag gatacatttc
     3061 tattgagact ggatacgata ccttgagtga cagtatttgc atattcagaa cctaacgggc
     3121 taccgatggc aatagcagtt tctcctacag ttaacttact agaatcacca aactcagcta
     3181 ctgttgtcac tttttctgaa gagattttga cgacagcaat atcagagaaa gtgtcagctc
     3241 cgacaatttc tccaggtact ttagtcccat ctgacaatcg aatatctact ttgctggcgc
     3301 catttataac gtgattgttg gtgacgatgt aagcttcttt atcattcttt ttataaataa
     3361 ctccagatcc ttcactagag attcgctgag aatctgtgtc agtatcatca ttgccaaata
     3421 cgctattttg tctgtttgcc gaataagtaa taacagaaac aacagcatct tttactttgt
     3481 taacagcctg tgttgttgaa ttttcgttct tataggcagt ttgtgtaata gtactattgt
     3541 tgttagagtt gtttacacta cttttttgag ttagttgagt tattgaaaaa ctacccaagg
     3601 ctccactaaa aaagctaatg acgataacga ctaataattg aaaccatttt ttgtaaaatg
     3661 tttttagatg tttcatattt gcctccatat gtttgaatta ctgaaagtat aaactgacta
     3721 gcttaattat aacttaaaca caaaagtttt acacaaactg tggataactc ttttgaaact
     3781 gtgattttct taattgaaat ctatttttta ttttgtgaat aagatgtgaa aaaatagaga
     3841 atatgttaga atagagtcat gaaaattaaa gttgtaacag ttgggaaact gaaagaaaag
     3901 tatttaaaag atggtatcgc agagtattca aaacgaattt ctagatttgc taagtttgaa
     3961 atgattgagt tatcagatga aaaaacacca gataaggcca gtgaatcaga aaatcaaaag
     4021 attttagaaa tagaaggtca gagaatttta tcaaaaattg ctgaccgtga tttcgttatt
     4081 gtgttagcca ttgaagggaa aactttcttc tcagaagaat ttagtaagca gttagaagaa
     4141 acttctataa aaggattttc tactcttact tttattattg ggggaagttt aggattgtca
     4201 tcatctgtaa aaaatagagc caatctttct gtcagttttg gtcgcctaac cttacctcat
     4261 cagttaatga gactagttct tgttgaacaa atctatcgcg cttttacgat tcagcaggga
     4321 ttcccctacc ataaatagaa aattgacttt taattgaatt tttggtagaa taattgtgtt
     4381 aggtctcata gctcagctgg atagagcatt cgccttctaa gcgaacggtc gcaggttcga
     4441 atcctgctgg gatcaatata atagcaaagc tgggaatttt cccggctttt ttcttaaaaa
     4501 agtacacttt gggagaaaaa aatgacagtt gagagaattt tatctaaaac gaaattccat
     4561 tttgtataat ggtttttgta agttagctta caagaaaaaa acattttagg agattttatt
     4621 atgaaaaaca cagttaaatt ggaacagttt gtagctttga aggaaaaaga cttacaaaag
     4681 attaaaggtg gggagatgag gctgtcaaaa ttcttccgtg attttatttt acaaagaaaa
     4741 aagtaatgaa ataaggggaa agagtaatgg atttatttgg atttgggaca gttattgttc
     4801 atcttttaat tattagtcac aactaccatt taatttgtaa aggtcaaata aatagaaaag
     4861 aattattcgt ttttggtgct tatacattac taacagaaat agtatttgat tttcccttat
     4921 atattctata tttagatggg ttagggattg caatattttt atttcctttg ggcttatatt
     4981 cctattttcg atggatgaaa cagtatgaga gggatagagg actattccta agtttactac
     5041 tatctctttt atatgagagc actcataact ttctgtccgt aactttctcc tctataacag
     5101 gagataattt tgttttacaa tatcatttcc cattcttttt cgttgtgacg gtgttaacct
     5161 attttgttac attaaaaatc atttattatt tccatttgga actagcctat tttgacaagg
     5221 actaccttta tcctttcttg aaaaaagtat tttttgcttt actattgcta catattgtat
     5281 ctttcgtttc agatatggta agtacgatta aacatttgaa tagttttgga agtattttgt
     5341 catctattgt ctttatctct ctccttttga ccttctttgc aatgaattct cataaagttc
     5401 aaatggagaa agagattgct ttgaagcaga agaaatttga acagaaacat ttacagaatt
     5461 acacagatga aattgtcggt ctgtataatg aaatccgtgg ttttcgacat gattatgctg
     5521 ggatgcttgt cagcatgcag atggcaattg acagtggtaa tttacaggaa attgacagaa
     5581 tttacaatga agttttagtc aaagcaaatc ataaattgcg ttcagataag tacacttact
     5641 ttgatttgaa caacatagaa gactcagctt tacgaagttt ggttgctcag tcaattgtct
     5701 atgctcgaaa taatggtgta gagtttacac tggaagtaaa agatacgatt accaagcttc
     5761 caattgaact attggatttg gttcgtatca tgagcgtttt attgaataat gctgtcgaag
     5821 gatcggctga tagctataaa aagcagatgg aagtagcagt tattaagatg gaaactgaaa
     5881 cagttattgt gattcagaat tcatgtaaaa tgacgatgac tccttcagga gatctatttt
     5941 ccttaggatt ctccactaag ggaagaaatc gcggagtcgg attaaataat gtgaaagaac
     6001 tactagataa gtacaacaat attattttag aaacagagat ggaaggcagt acatttagac
     6061 aaatcattag atttaagagg gaatttgaat gaaagtttta attttagaag atgttattga
     6121 acatcaagtg agactagaga gaatattgga tgaaatttcg aaagaatcga atattccaat
     6181 atcatacaag acaacgggaa aagtccgtga atttgaagaa tacattgaaa atgatgaagt
     6241 aaatcagctt tatttcctag atatcgatat tcatggaatt gagaaaaagg gatttgaagt
     6301 ggctcagttc attcgtcatt acaatcctta cgctattatc gtctttatca ctagtcgatc
     6361 agagtttgcg actctaacct ataaatacca ggtatcagcc ctagattttg ttgataagga
     6421 tatcaatgat gagatgttta agaagagaat tgagcaaaat atcttctaca cgaagagtat
     6481 gttacttgaa aatgaagatg ttgtagatta tttcgactac aattacaagg gaaatgattt
     6541 aaaaattcct taccatgata ttttgtatat tgaaacaaca ggggtatctc ataaattgcg
     6601 cattattggt aagaattttg caaaagagtt ttatggtacc atgacagata ttcaggaaaa
     6661 ggacaaacat actcagcgat tttattctcc tcacaagtca tttttggtaa atataggcaa
     6721 tatcagagaa attgatcgaa aaaacttaga aattgttttc tatgaagacc atcgttgtcc
     6781 tatttcaaga ttaaaaatta gaaaattaaa agatatttta gagaaaaaat ctcaaaagtg
     6841 attgacaatt agcaagaaat tgatataatg gttatatctg ctacagatag aaggtccgtt
     6901 ggtcaagggg ttaagacacc gccttttcac ggcggtaaca cgggttcgaa tcccgtacgg
     6961 actattttat ccgccatagc tcagttggta gtagcgcatg actgttaatc atgatgtcgt
     7021 aggttcgagt cctactggcg gagtatagat aaaagggaac acagctgtgt tcctcttttt
     7081 gtatcaattt gtatcaccaa gcattttcat aaggaagtct gttatttctt gagaactttc
     7141 tttttttcca tgtgcaatcc aagtttggca gacaccaaaa agtgcatgag ttagatagat
     7201 gctactatat tctaattcag tggtatttag attcagttgc ataaatcgct tttgtaaatc
     7261 tgtactgagc atgatatgaa gtttatttcg taagaaattt tggatttctt tagtcccatt
     7321 ttcagaaaga agggcagcca gaagtggttc tgactctaga tattcaaaaa cttctaaaat
     7381 agcgtctctt ttgtgatgag catgtttttg aaaaatatat tcaaatgtat ggaatagctt
     7441 gctttgatag tgctcaatca tatcatactt atccttatag tgagtataga agctggaacg
     7501 actaattccg gctttttcta ctaatttgac agtagaaatt ttatcaaatg gctgttccat
     7561 cagtaattgt accatagcgt tttcaatagt tcgctttgtt tttaagcgtt tgttactttc
     7621 ttgcatattt cctccttgta aacaaattag actatatgtc taaaaataga ttttttatct
     7681 tgtaatttag attttttaat gtataatcta ttatatcaaa attttagaca atatgtttaa
     7741 aaaaggagaa actatgttta aagaatggaa agcaatttaa aaaaaaacaa cctttattat
     7801 tgtcatgatc gggatttctc ttattccagc tctgtacaat atcatatttt tgtcatcaat
     7861 gtgggagcca tatgggcaat tgtctgactt acctgtggca gttgtaaata atgataaaga
     7921 ggcttcctat aatggtaata ctatggcaat aggaaaagac atggtgtcca atttaaaaga
     7981 aaataaaacc ttggattttc attttgtaga tgaagaggaa ggaaagaagg gattggaaga
     8041 tggcgattac tatatggtag tgactttacc aagtgattta tctgaaaaaa caactacatt
     8101 atccaatatt caatcgacag cagcttatca atcattgaca agtgagcaac aaactgagat
     8161 aagtgattct gtatctcaaa attcaactga tagtattcaa tcggctcagt caattgtagc
     8221 tttagtacaa ggtttacagg gaagtttaga aaacttacaa aatcaatctt ctaatctttc
     8281 gactttaaaa aatcaagcta atcaagtatt acctattact tctacttctt tgataggatt
     8341 gtcaagtgga ttaacagaga tacaaggaga tgttactagc aaattagttc ctgccagtca
     8401 gtcgattgca tcaggtgtaa acgcatatac tacaggtgtt gataaagttt ctcagggcgc
     8461 aagtcaacta agtgaaaaaa atgccacctt gacaggtagt ttggataaac tagtttcagg
     8521 ctcaaacacc ttgacacaaa aatcttctag attgacagca ggagttggtt aattacaatc
     8581 aggatctggg caattagcag acaaatccag tcagttactt tcaggtgctt ctccattaga
     8641 gaatagagct aataaattgg cagatggatc taggaaacta gcagaaggtg gaacaaagtt
     8701 aacttctgga ttggaagatt tacagacagg acttgcttct ttaggacaag gactaggtaa
     8761 tgctagtgat caactcaaat cagtatcaac agaatctaaa aatgcagaga ttttgtcaaa
     8821 tccactcaat ctttcaaaaa cagacaatga tcaagttcct gtaaatggaa tcgcaatagc
     8881 tccttatatg atatcagttg ctcttttttt tgcagcaata tcaacaaata tgatatttgc
     8941 gaaattgcct tcaggacgtc atccagagag ccgttgggct tggttgaaat cttgagctga
     9001 aataaatggt attatagctg ttttggcagg aattttggta tatggaggag ttcagcttat
     9061 tggtttaact gctaatcatg agatgagaat atttattctc atcatcctaa caagtttagt
     9121 attcatgtct atggtgacca ctttagcaac gtggaatagc cgtataggag cttttttctc
     9181 acttattttg cttttactac agttagcatc aagtgcaggt acttatccac ttgctttgac
     9241 aaatgatttc tttagatcta ttaatccctg gttaccaatg agctattcag tttcgggatt
     9301 acgacaaaca atctctatca acaagtcatt ttcctagctg tcatactagc tctatttact
     9361 ggtttaggta tgctagccta tcaacataag aaaatggaag aagattaaaa aaatcgaccg
     9421 attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata gattccaaat
     9481 agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg aatgaaggaa
     9541 agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt ttgagcttgt
     9601 ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata aggaatggtg
     9661 aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt tttagaagtt
     9721 gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt tttggtatct
     9781 aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa actaacattg
     9841 atattgaaag tttttttctc tttagtatat cctagattaa agaaggagaa gacattatca
     9901 gttgtaaaag tctttttttc accatttaca aggatgtcaa ccttcttttg tttatcgtta
     9961 gaaaagtgaa ggtttatgaa agagagataa acttggctgt tttctggaac ttcaatttga
    10021 tactggattg ctgcatcttc atttgaagaa cttgtgacac taatcaaatc attagtattt
    10081 tctatttttt ctgttttttc ataaggtatt ggagaaaaat aatcaaaatt gacgttagca
    10141 agttgattta aaaatgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg
    10201 taaacagatt gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga
    10261 ttatcttttt gatagatatc tttaaagcca tacttatcaa taggactgtc tgagatattg
    10321 tactggatac caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga
    10381 ttagtcccag aggatttaaa accaagttta tctaaagtag agcttgatga acgatttcga
    10441 acagatgaaa attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga
    10501 gtttgtagtt tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata
    10561 gctgggatat ctcgactata agcacttcga gaagcaaatc cccattcctt agcaattccg
    10621 tccatttgag atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg
    10681 gcaaatagat tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca
    10741 accaaaaatt caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt
    10801 agatagatgg tagctaaaaa tcctgctact acaagaaaaa gcgaaactaa aaaattccag
    10861 actttaagtt ctttcagacg atttaagact tctgctgctg tgtaaattaa caaggtagag
    10921 aaaatccaag catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag
    10981 tcaagagctt ctatgtaaaa gcttgcaatt agaaatgcaa agaatattac atatatgagt
    11041 ttcacgtgaa acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaagggaagt
    11101 agtccaacaa aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat
    11161 tgcttagcaa agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc
    11221 aatttttccc catgtgtctg taaatcaaac agagtgggaa gagtcataat caaactagcc
    11281 ataccagcta aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag
    11341 tcccacgaaa tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa
    11401 taataatttt gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca
    11461 gttatcagta gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag
    11521 gtttttatct ctaattgact gacagtgaaa ctcatcagag cataggaagt agataaggct
    11581 agttttaaaa tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga
    11641 ccaatcaatc caaattttaa gagagttgtc agatagatag catctggcat attcgttaga
    11701 tcaaaaaagt aaaccagagg cgcgagaaaa ctacccaagt aataactaga tagggcatag
    11761 aagtttagcc ctagaccact tgtaaaggtg taaaacagat tactatttcc atgtaggata
    11821 tttcgtaagg ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag
    11881 ttgtcgctat tccagtagat accttgagat agatatactc cagacataat cactacagga
    11941 atgatgaaag aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt
    12001 agaatgatag aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt
    12061 ccaaagttct ttaacttttg cttgtatttc tgcattttct aggaattcat cgtaggtttc
    12121 atcgatacgg tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg
    12181 aataaattcg tggtcatggc tggcaaagat gattgattct ttaaagtttt tcaatccatc
    12241 attcaagctt gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac
    12301 atttgatttt aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa
    12361 gacatttaca ggtttgttaa cttcatctcc agagaagagc atacggccga ggaagccacg
    12421 taggaaagta ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga
    12481 ttctcctcct gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac
    12541 tccccacttg acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt
    12601 cgtttgaata tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact
    12661 aatattatcc aagatagttt caccatcaat ctttacagtt aaattttcta ctgtcaagag
    12721 atcattacca atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg
    12781 cacaatctct tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga
    12841 cttagaagca ttggcagaga aacgagcaac aaattcttgc aattgtttaa ttttttcttc
    12901 tgctttagca ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa
    12961 gtcgtagttt ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca
    13021 aactttgttt aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat
    13081 caagaagtct tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa
    13141 gagaagaaca tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc
    13201 gttggccaat tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag
    13261 tagttgagag gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag
    13321 ttcggcagca cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt
    13381 ctctttcatg atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc
    13441 atcttcgtag tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga
    13501 gatgtgacca gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc
    13561 ggcaccatta gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc
    13621 atcaaaaagt ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatgtttt
    13681 tctcctatat gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat
    13741 ttgtcaattt tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag
    13801 gctgatttat tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac
    13861 tatatcgaag gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg
    13921 aaaattgcat attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga
    13981 ggataagtat gatatgtttg tgttcttggc tgaccaacaa gccttgacag atcatgccaa
    14041 agatcctcaa accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg
    14101 attggatcca aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt
    14161 gtctatgtat tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa
    14221 gacagagatt tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc
    14281 aatcgctcaa gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga
    14341 tcagaaacca atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa
    14401 ctgtgatgtc ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt
    14461 gcctggttta gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc
    14521 tgatgatgcg gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat
    14581 ccgcgttgag gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt
    14641 tggtcgtcca gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg
    14701 tcttggtgat gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc
    14761 tattcgtgag cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca
    14821 aaaaggtagt gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc
    14881 aatgggactt cattacttta actaagttta ttttacaaga aactatcttt tctatctcga
    14941 agaaaagata gttttttatt tacccctgta acatttgaca aatttttata gaatggtatg
    15001 atagatacaa tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa cttcgaatgt
    15061 ctaattggga cactaaattt ttgaaaaaag gttttacctt tgatgatgta ttgcttattc
    15121 cagctgaaag tcatgtgttg cctaacgatg cagatttaac aactaaattg gcagataatt
    15181 tgactttaaa tatcccaatt attaccgctg ccatggacac agttacagag agtcaaatgg
    15241 ccattgctat tgctcgtgca ggcggtctcg gagttatcca taaaaacatg tcaattgctc
    15301 aacaagcaga cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt attgatccgt
    15361 tcttcttgac gcctgaacat acaattgctg aagcagatga gcttatgggt cgttaccgca
    15421 tcagtggtgt tccagttgtt gaaacacttg aaaatcgtaa attggttggt attttgacaa
    15481 accgagatct tcgttttatt tcagattata atcaaccaat ttcaaaccat atgactagtg
    15541 aaaatcttgt tactgctcct gtgggtacgg atcttgcaac ggctgagagt attcttcaag
    15601 agcatcgtat tgaaaaactt ccgttggtcg atgaagaagg cagtctttct ggtttgatca
    15661 ctatcaaaga tattgaaaaa gttattgagt ttccaaatgc agctaaagat gagtttggtc
    15721 gtcttctagt tgcaggtgca gtaggtgtta cttcagatac atttgaacgt gcagaggctc
    15781 tttttgaggc aggagcggat gcgattgtta ttgatactgc acatggtcat tctgcaggtg
    15841 tcttgcgtaa aattgccgag attcgtgctc atttcccaga tcggactttg attgctggaa
    15901 atattgctac tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac gttgttaagg
    15961 ttggtattgg accaggttct atctgtacta ctcgtgtgat tgctggtgtt ggtgttccgc
    16021 aagtaacagc tatctacgat gctgcagctg ttgcgcgcga atatggtaaa acgattattg
    16081 ctgacggtgg gatcaagtat tctggagata ttgtaaaagc acttgctgca ggtggaaatg
    16141 ctgttatgct tggatctatg tttgctggaa ctgatgaagc tccaggcgaa actgaaatct
    16201 tccaaggacg taaattcaag acttaccgtg gtatgggatc aattgctgct atgaagaaag
    16261 gttcaagcga ccgttatttc caaggttctg tcaatgaagc aaacaagctt gttccagaag
    16321 gaattgaagg tcgtgttgct tataaaggag cggcagctga tattgttttc caaatgattg
    16381 gtggtattcg ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa ctacacgata
    16441 atgctcaatt tattgaaatg tctggtgctg gtttgaaaga aagccatcct catgatgtgc
    16501 aaattactaa tgaggcacca aattattcta tgtaaaaaac aatgaaaaga actccagtga
    16561 aaacaggagt tcttttacaa tgttgtcaat ttccatttac agcagcttta ccatcctgaa
    16621 tagtgaagat acttagattt tctggcagat tttgaagatg gtctaagctt gttgttgtga
    16681 taaaggtttg gattgattga gaaatcgttt ctaataattt taactgtcta gtgttgtcaa
    16741 gttcactcat cacatcgtca agcagtaata taggagattc tgtggtaatg ctttccatta
    16801 attcgatttc tgctaatttt atcgagagga cgagactacg atgttgacct tggcttccga
    16861 aactagcatc catcccattt atataaaaag aaatgtcatc tcgatgagga ccgacaccag
    16921 tattcttttt aaataaatct ctggatctac ttttttctaa agcaattttg aaagattcgg
    16981 ataagttttg tttgtcagtt atattgacag aagattgata ggatattgac aactcttcga
    17041 tctgattaga gagttcaaaa tgtttcttac gcccaaatga ttctagtttt tttatgaaat
    17101 ctaagcggtg attcattaca cgacatccat aatcaactag ctgatcatct aacacagaaa
    17161 ggaatgtttc atctattttt tgagctgatt ttaggtaagt gtttctttgc tttaggatgt
    17221 ggttataatt ggttaagtca gataaataga ttggcttaat ttgcccaagt tccatatcaa
    17281 tgaattttcg tcgaatcgaa ggtgctcctt taattagttg taaatcttca ggagcaaata
    17341 agacaacatt catgtgtcct acataatctg aaaggcgtgc ctgttttaag tgattaactt
    17401 ttgttacacg acctttttgt gttagttcga tttctagggg aatggatcca gtttttttct
    17461 gaacgagacc tgaaagatga agttgttcct catcaaaatg aatgagattt ttatctgttc
    17521 gagttcgatg actacgcgtt aaggctaaaa aatagatagc ctctaacatg tttgtttttc
    17581 cttgtgcatt acgtcccaaa aagacattca atttaggatt aaagtctatt ttcgtttctt
    17641 tgtaattacg aaaagtcttg agagatagat gttgtagcca catgattatc taccagggaa
    17701 tcgtggagct tgttggcttt taggtgatga agcaggtttc gatttgtctt ttttaactcc
    17761 cttattcatc tttttgacaa gtttagcgat ccgttctttt tcaactttat cagcttggta
    17821 ttcatcttgt tcttcagaag taggttgtgt caacaagatg tcaatgttca tgtcagggat
    17881 gtcaacttta tcgccaatac gaagtttttt tccacgacgg ctctctaatt ccctattaaa
    17941 gtaaacagaa tgttcagaga gaaatgattt aatagctcct ccgctatgtg taattccaag
    18001 ttctttgagt agtgcttgga gggtaataaa ttcttcaaat aatttgtatt ccataattca
    18061 cctcaatctt tggtattata ccatattttc ctaaaaatag tgagagagac agcgagaaat
    18121 gtaagcgatt tttatttcaa aagtgttaca gggaacaaga aaatttcagg ttttcgtgat
    18181 ataatagaag tctgtatata aggaggtaaa tcatggagtt agtgcatgga atttcaacac
    18241 attttatcca atcaaaaaag tttaaaacaa acaaaattac cgtgcgtttt accgctccat
    18301 tatcccttga tacgattgca ggtcacatgt tgagtgcaag tatgctagag actgctaatc
    18361 agatgtaccc cacttctcaa gatttgagga gacacttggc cagtctatac ggtacagata
    18421 tgtcaaccaa ttgtttcaga agagggcaaa gccacattat agaattgaca tttacctatg
    18481 ttcgtgatga gtttttaagt aggaaaaacg tgctaacctc tcagattttg gaacttgtaa
    18541 aagaaactct tttttcaccc gtagtagttg ataatgggtt tgatccggcc ttatttgaaa
    18601 ttgagaaaaa acaattgcta gcaagtttag cagctgatat ggatgattct ttttattttg
    18661 cacataaaga attggataaa ttgttttttc atgatgaacg tcttcaattg gaatatagtg
    18721 atttacgaaa tcgtatttta gctgaaactc cacaaagttc ttattcttgt ttccaagaat
    18781 ttttagccaa tgatcgaata gatttctttt tcctaggtga ttttaatgag gttgaaattc
    18841 aaaatgtatt agaatcattt ggctttaaag gtcgaaaagg agatgtgaag gttcagtatt
    18901 gtcaacctta ttctaatatc cttcaggaag gtatggttcg gaaaaatgtg ggacaatcca
    18961 ttttggaatt aggttatcat taccgttcta aatatggtga tgaacaacat ttacccatga
    19021 ttgtaatgaa tggtttactt ggtggatttg ctcactctaa gctctttaca aatgtccgtg
    19081 aaaatgctgg attagcttat accatttcaa gtgagcttga tttatttagt ggattcttga
    19141 ggatgtatgc tggtatcaat cgagaaaatc gtaaccaggc tcgtaaaatg atgaataatc
    19201 aactgcttga tttaaaaaaa ggttatttta cagagtttga gttaaatcag accaaggaaa
    19261 tgattcgttg gtcgttgtta ctttctcaag ataatcaatc ttcattgatt gaacgtgctt
    19321 atcaaaatgc cttatttgga aaatcttcag cagactttaa aagttggatt gcaaagcttg
    19381 aacaaattga caaagatgct atttgtagag tagctaataa tgtgaaacta caagcgattt
    19441 actttatgga aggaatagaa tgacaaaggt tgtttttgaa gaaaaatact atccagctgt
    19501 aaaagaaatg gtttatcgaa ctcgtttggc caacggattg acagttgctc ttttgcctaa
    19561 aaaggaattt aaagaggttt acgggagtgt cactgtacag tttggttcgg tagatacgtt
    19621 tgtcacagaa gttgacggag atgtaaaaca atatcctgga ggaattgctc attttcttga
    19681 acataaatta tttgagagag aagattctag tgatttgatg tcggctttta cgagtctagg
    19741 tgcagatagt aatgccttta caagctttac aaaaacaaac tatctttttt cagcaacgga
    19801 ttatttttta gaaaatttag atttacttga tgaattggta acatcagcac actttactga
    19861 agcttccatt ctgacagagc aggatattat tcagcaagaa cgagaaatgt accaagatga
    19921 tccagattcg tgtttattct tttcaacttt agcgaatttg tatcctggta cacctttagc
    19981 aactgatata gttggaagtg aggagtccat ttcccaaatc aatctaacta atttgcaaga
    20041 aaattttaca aagttttaca aacctgtaaa catgtctctg tttttagttg gtaattttga
    20101 tgtggagcga gtacaggact attttgaaag caaagaactg aaagattcag attttcagga
    20161 agtagcaaga gaaaagttgt ttttacagcc tgtaaagcca acagatagta tgagaatgga
    20221 agtatcttct cccaaactag cgattggagt tagaggtaag cgagaagttt ctgaagcgga
    20281 ttgctatcga catcatattt tattaaaatt attgtttgca atgatgtttg gttggacttc
    20341 ggatcgtttt caaaaatgtt atgaatcagg taaaattgat gcgtccttat ctctggaagt
    20401 tgaaataaca agtcgctttc attttgtcat gttgacaatg gatacgaaag agccagttgc
    20461 tttgtctcat caatttagga aggctattcg taattttaca aaggatttag atattacaga
    20521 ggaacattta gatattatca aaagagagat gtttggcgaa tttttcagta gcatgaactc
    20581 tcttgaattt attgcaacgc aatatgatgc ttttgaaaat ggtgagacaa tttttgattt
    20641 gccgaaaatt ttacaggaaa ttactttaga ggatgtcctt gatgctggac atcatttaat
    20701 agatgatggt gacatagttg attttacaat attcccatcg tagtaaccta ttataataga
    20761 cactagaaag aagggatgac aagtatgaga aaaaaaacaa ttggagaggt tttacgatta
    20821 gctagaatca atcagggatt gagtttagat gaattgcaga aaaagacaga aatccagtta
    20881 gatatgttgg aagcaatgga agcagacgat ttcgatcaac ttccaagtcc tttttacacg
    20941 cgttctttct tgaaaaaata tgcatgggct gttgagttag atgaccaaat tgttttggat
    21001 gcttatgatt ctgggagtat gattacttat gaggaagtag atgttgatga agatgagttg
    21061 acaggtcgta gacgttcaag taagaaaaag aagaaaaaaa tatcattttt acctttattt
    21121 tattttatcc tttttgcttt atcgatttta atttttgtga cttattatgt ttggaactat
    21181 attcaaactc aaccagagga gccttctctt tctaattaca gtgtggttca atcaacaagt
    21241 tcaactagct ctgttcccca ctcctcaagt agtagttctt ctagtataga atcagctata
    21301 agtgtatcag gcgaaggaaa tcatgtagaa atcgcttata agacaagtaa ggaaacagtt
    21361 aaattgcaat tggcagtttc agatgttaca agttgggtca gtgtttcaga aagcgaactt
    21421 gagggcggtg taaccttatc gccaaagaag aaaagtgcag aagcaacagt tgcaactaaa
    21481 agtcctgtaa caattacgtt aggtgttgta aaaggtgttg atttgacagt agataatcag
    21541 actgttgatt tatcgaaatt aacagctcag actggacaaa tcactgtaac ctttactaaa
    21601 aattaaggaa aaacgaatga aaaaagaaca aattcccaat ctcttaacaa taggtcgaat
    21661 tctctttata cctattttta tctttatttt aacgatagga aattcgatag agagtcatat
    21721 agttgcagct attatctttg ctgttgccag tattaccgac tatttagatg gatatttagc
    21781 tcgtaaatgg aatgtggtca gtaattttgg taaatttgca gatcctatgg cggataagtt
    21841 actagttatg tcggctttta ttatgttgat tgagttaggt atggctccgg cttggattgt
    21901 tgcagtgatt atctgtcgtg agttagctgt gacaggttta aggcttttat tggttgaaac
    21961 tggtggaaca attttagcag cagcaatgcc tggaaaaatt aaaactttta gtcagatgtt
    22021 tgccattatt ttcttgctat tacattggac tttgcttggt caagttctac tttatgtagc
    22081 cttatttttc actatctact ctggctatga ctatttcaag ggtagtgcct atgtatttaa
    22141 agggacattt ggttcgaaat gaaatcaata attgatgtaa aaaatctttc ttttcgctat
    22201 aaagaaaatc agaactacta cgatgtgaag gatattacgt ttcacgtgaa acgtggagaa
    22261 tggctttcga ttgtagggca taatggtagt ggtaaatcaa cgacggttcg attaattgat
    22321 ggcttactgg aagcagaatc cggagagatt gtaattgatg gccaacgttt gactgaggaa
    22381 aatgtttgga atatacgtcg tcaaatcggt atggtttttc aaaatccaga caatcaattt
    22441 gttggagcga ctgttgaaga tgatgttgct tttggtttgg aaaatcaggg actttctcgt
    22501 caagaaatga aaaagagagt ggaagaagct ctggctttag ttggcatgtt ggactttaaa
    22561 aagagagagc cagcgcgtct atcaggtggc caaaagcaac gtgtggccat tgcaggtgtt
    22621 gtagccctaa gaccagctat tttaatctta gatgaagcaa cgagtatgtt ggatcctgag
    22681 gggcgtagag aacttattgg gacagtaaaa ggaattcgaa aagactatga tatgacggtc
    22741 atttctatta cccatgattt ggaagaagtc gccatgagtg atcgcgtatt ggtcatgaaa
    22801 aaaggggaaa ttgaatcaac tagtagtcca agggagcttt tctctcgaaa tgatttagat
    22861 caaattggat tagacgatcc ttttgctaat caattaaaaa aatctttgag ccagaatggc
    22921 tatgatttac ctgaaaatta tttgacagaa agtgagctag aggataagct atgggaattg
    22981 ctctagaaaa tgtgaatttt acatatcaag aaggtactcc cttagcttca gcagctttgt
    23041 cggatgtttc tttgacgatt gaagatggct cttatacagc tttaattggg cacacaggta
    23101 gtggtaaatc aactatttta caactcttaa atggtttatt ggtgccaagt caagggagtg
    23161 tgagggtttt tgatacctta atcacctcga cttctaaaaa taaagatatt cgtcaaatta
    23221 gaaaacaggt tggcttggta tttcagtttg ctgaaaatca gatttttgaa gaaacggttt
    23281 tgaaggacgt tgcttttgga ccgcaaaatt ttggagtttc tgaagaagat gctgtgaaga
    23341 ctgcgcgtga gaaactggct ctggttggaa ttgatgaatc actttttgat cgtagtccgt
    23401 ttgagctgtc agggggacaa atgagacgtg ttgccattgc aggcatactt gccatggagc
    23461 cagctatatt agtcttagat gagccaacag ctggtctaga tcctctaggg agaaaagagt
    23521 tgatgaccct gttcaaaaaa ctccaccagt cagggatgac catcgtcttg gtaacgcatt
    23581 tgatggatga tgttgctgaa tatgcgaatc aagtctatgt aatggaaaag ggacgtttag
    23641 taaagggggg caaaccaagt gatgtctttc aagacgttgt ttttatggaa gaagttcagt
    23701 tgggagtacc taaaattacg gccttttgta aacgattggc tgatagaggc gtgtcattta
    23761 aacgattacc gattaagata gaggagttca aggagtcgct aaatggatag tatgatttta
    23821 gggcgttata tcccagggga ttcgattgtt caccgattgg atccacgtag caaattgctt
    23881 gctatgatgc tactgatttt gattgtgttt tgggctaata accccttgac gaatctgatt
    23941 ctttttatag cgacagggat atttattgcc ttgtcaggag tatctctttc attttttatt
    24001 cagggcttga agtctatgtt tttcttgatt gccttcacaa ctatttttca actatttttc
    24061 atttctaatg ggaatgtttt atttgagttt tcgtttgtga gaatcacgga ttatgctttg
    24121 caacaagctg ggattatttt ttgtcgcttt gtattgatta ttttcttttc aactttgtta
    24181 accttaacaa ccatgccttt aagtttggca tcagctgtcg aagctttatt agcaccttta
    24241 aagcgtgtga aagttccagt tcatgaaatt ggattgatgc tgtctatgag tttgcgtttt
    24301 gtcccaacct tgatggatga tacgactcgg attatgaatg cacagaaagc tcgtggagtg
    24361 gattttggag aaggaagcat cgttcaaaaa gtaaaggcga tgattcccat tttgattcct
    24421 ctttttgcga caagtttaaa acgtgcagat tccttggcta tcgctatgga agcgcgtggc
    24481 tatcagggtg gaaaaggcag aagtcaatac cgacaattga aatggactct gaaggatacg
    24541 ctgaccattc ttgttattct cgtacttggt tgttgtttat ttttcttaaa atcttagtag
    24601 ctttcaggaa ttgataaaaa gttactgtaa cagtttttga tataaaggta ggaatatgaa
    24661 ccgttttaaa aaatcaaaat atgtcattat tgtttttgtc actgttctgc ttgtgtcagc
    24721 tctcttagcg acgacttatt caagtacaat tgtgacaaaa ttaggagatg gaatctcatt
    24781 ggttgataga gttgtacaaa aaccttttca gtggtttgat tctgtcaaat cagatttggc
    24841 tcatttgaca cgaacatata atgaaaatga aagtttgaag aaacagcttt accaattaga
    24901 agttaaatca aatgaggtgg aaagtttaaa gacagaaaat gaacaactgc gccaattgct
    24961 tgatatgaag tctaaattgc aagccacaaa gactttagca gcagatgtta ttatgcgttc
    25021 tccggtatct tggaagcagg agttgacctt agatgcaggt agatcaaaag gtgcttctga
    25081 gaacatgtta gctattgcaa atggtggctt gattgggagt gtttcaaaag tagaggagaa
    25141 ctctactata gtcaaccttc tgacaaatac ggaaaatgct gataagattt ctgttaaaat
    25201 tcaacatggc tctactacaa tttatggaat tattattggc tatgacaagg aaaatgacgt
    25261 tcttaaaatt agccaattaa atagtaatag cgatattagt gcgggagata aggtgactac
    25321 tggtggatta ggaaacttta acgttgctga tattcctgtt ggtgaagtgg ttgccacaac
    25381 gcatagtaca gactatttga cacgagaagt aactgttaaa ttgagtgcag atactcataa
    25441 tgtagatgtg atagaattag tggggaattc ataatgagac agttgaagcg agttggagta
    25501 tttttattgc ttcctttctt tgttctaatt gacgcccata ttagccagct tctgggctca
    25561 tttttccccc atgtacattt ggctagtcat tttctttttc tatttctctt atttgagacg
    25621 atagaagtat cagagtatct ctacctagtc tattgttttg ttataggctt ggtttatgat
    25681 gtttactttt tccatctaat agggattaca actctcttat ttatcttatt gggagccttc
    25741 cttcataaat tgaatagtgt tattttgttg aatcgttgga caagaatgct agctatgatt
    25801 gtgctgacat tcctgtttga aatgggtagt tatcttttgg cttttatggt agggttgaca
    25861 gtagatagca tgtcgatttt tatagtctat agcttggtac cgacgatgat tttaaatttt
    25921 ttatggatta ctgtttttca atttattttt gaaaaatatt atctataaga acgacatata
    25981 aatgtaacaa aggcgtaata tttattaggc ctttttttgg tatactagta ttgtctttaa
    26041 aagaaggagt atctacgtaa tatgaagaaa aaaatcttag cgtcactttt attaagtaca
    26101 gtaatggttt ctcaagtagc tgttttaaca actgcgcatg cagaaacgac tgatgacaaa
    26161 attgctgctc aagataataa aattagtaac ttaacagcac aacaacaaga agcccaaaaa
    26221 caagttgacc aaattcagga gcaagtatca gctattcaag ctgagcagtc taacttgcaa
    26281 gctgaaaatg atagattaca agcagaatct aagaaactcg agggtgagat tacagaactt
    26341 tctaaaaaca ttgtttctcg taaccaatcg ttggaaaaac aagctcgtag tgctcaaaca
    26401 aatggagccg taactagcta tatcaatacc attgtaaact caaaatcaat tacagaagct
    26461 atttcacgtg ttgctgcaat gagtgaaatc gtatctgcaa acaacaaaat gttagaacaa
    26521 caaaaggcag ataaaaaagc tatttctgaa aaacaagtag caaataatga tgctatcaat
    26581 actgtaattg ctaatcaaca aaaattggct gatgatgctc aagcgttgac tacgaaacag
    26641 gcagaactaa aagctgctga attaagtctt gctgctgaga aagcgacagc tgaaggggaa
    26701 aaagcaagtc tattagagca aaaagcagca gctgaggcag aggctcgtgc agctgcggta
    26761 gcagaagcag cttataaaga aaaacgagct agccaacaac aatcagtact tgcttcagca
    26821 aacactaact taacagctca agtgcaagca gtatctgaat ctgcagcagc acctgtccgt
    26881 gcaaaagttc gtccaacata cagtacaaac gcttcaagtt atccaattgg agaatgtaca
    26941 tggggagtaa aaacattggc accttgggct ggagactact ggggtaatgg agcacagtgg
    27001 gctacaagtg cagcagcagc aggtttccgt acaggttcaa cacctcaagt tggagcaatt
    27061 gcatgttgga atgatggtgg atatggtcac gtagcggttg ttacagctgt tgaatcaaca
    27121 acacgtatcc aagtatcaga atcaaattat gcaggtaatc gtacaattgg aaatcaccgt
    27181 ggatggttca atccaacaac aacttctgaa ggttttgtta catatattta tgcagattaa
    27241 tttacagagg gactcgaata gagccctctt ttcaggtttt accgtgacaa tccctattaa
    27301 aaattatatc aaaatagctt gaaaatattg gaaaagtatg gtaaaatgaa aattgtcgtg
    27361 tgaacgataa tactcattct tgatgaattg tgaagcagtt gcccttgggt cgttttgcga
    27421 gttgaagtca agaagaggaa aaaaacaaaa aggagaaata ctcatggcag taatttcaat
    27481 gaaacaactt cttgaggctg gtgtacactt tggtcaccaa actcgtcgct ggaatcctaa
    27541 gatggctaag tacatcttta ctgaacgtaa cggaatccac gttatcgact tgcaacaaac
    27601 tgtaaaatac gctgaccaag catacgactt catgcgtgat gcagcagcta acgatgcagt
    27661 tgtattgttc gttggtacta agaaacaagc agctgatgca gttgctgaag aagcagtacg
    27721 ttcaggtcaa tacttcatca accaccgttg gttgggtgga actcttacaa actggggaac
    27781 aatccaaaaa cgtatcgctc gtttgaaaga aattaaacgt atggaagaag atggaacttt
    27841 cgaagttctt cctaagaaag aagttgcact tcttaacaaa caacgtgcgc gtcttgaaaa
    27901 attcttgggc ggtatcgaag atatgcctcg tatcccagat gtgatgtacg tagttgaccc
    27961 acataaagag caaatcgctg ttaaagaagc taaaaaattg ggaatcccag ttgtagcgat
    28021 ggttgacacc aatactgatc cagatgatat cgatgtaatc atcccagcta acgatgacgc
    28081 tatccgtgct gttaaattga tcacagctaa attggctgac gctattatcg aaggacgtca
    28141 aggtgaggat gcagtagcag ttgaagcaga atttgcagct tcagaaactc aagcagattc
    28201 aattgaagaa atcgttgaag ttgtagaagg tgacaacgct taatttatac aaatagtaat
    28261 tacctaggag ggcggggctt agcccggctc tcctattttc aaaaaatata ggagaattaa
    28321 aatggcagaa attacagcta aacttgtaaa agagttgcgt gaaaaatctg gtgccggtgt
    28381 tatggacgct aaaaaagcgc ttgtagaaac agacggtgac atcgaaaaag cgattgaatt
    28441 gcttcgtgaa aaaggtatgg ctaaggcagc taagaaagct gaccgtgttg ctgcagaagg
    28501 tttgactggt gtttatgtta acggtaatgt tgcagcagtt attgaagtaa acgctgaaac
    28561 tgacttcgtt gcaaaaaacg ctcaattcgt tgaattggta aatactacag ctaaagtcat
    28621 tgctgaagga aaacctgcta acaatgaaga agctcttgct ttgataatgc cttcaggtga
    28681 aactcttgaa gctgcatacg tatctgcaac agcaactatc ggagagaaaa tctcattccg
    28741 tcgctttgca ttgattgaaa aaacagacgc acaacacttt ggagcatacc aacataacgg
    28801 tggacgtatc ggtgttattt cagttgttga aggtggagac gaagcacttg ctaaacaatt
    28861 gtcaatgcac atcgcagcga tgaaaccaac agttctttct tacaaagaat tggatgagca
    28921 attcgttaaa gatgagttgg cacaattgaa tcacgttatc gaccaagaca acgaaagccg
    28981 tgcaatggtt aataaaccag ctcttccaca cttgaagtat ggatcaaaag ctcaattaac
    29041 tgatgatgtt attgctcaag ctgaagctga catcaaagct gaattggctg cagaaggcaa
    29101 accagaaaaa atctgggaca aaattattcc aggtaaaatg gatcgcttca tgcttgataa
    29161 cactaaagtt gaccaagctt acacacttct tgcacaagtt tacatcatgg atgacagcaa
    29221 gacagttgaa gcataccttg aatcagttaa cgcttcggta gttgagtttg ctcgctttga
    29281 agttggtgaa ggtatcgaga aagctgcaaa cgacttcgaa gctgaagttg cagctacaat
    29341 ggcagcagcc ttgaataact aataatagaa aggaagcaaa ttgcttcctt ttttgtatag
    29401 ggcttagatt tttcctgaaa ttgttttttc cccagtctca ctcgattaga ataattaaaa
    29461 caaaaagccc tataataagg gctaagggca tttaggagac tatacttcaa attcatagta
    29521 agcgcgttca taacaaggta tctatcctaa ttccccaact tccagtatac tagataaaaa
    29581 ataattatcg gaggaaaggt attgtactat gataattcaa ctaagtgatt taggtcaagt
    29641 tcaccttgtt tgtagcaaga tagatatgag gcagggaata gactcattag cctatgtagt
    29701 taaaacccac tttgaattgg atcctttctc cggtcaagtc tttctctttt gtggtggacg
    29761 taaagaccgc tttaaagccc tttactggga tggtcaagga ttttggctac tatataaacg
    29821 ctttgagaac gtcagactga cttggcccag tacagaaaag gatgtcaaag ctctcacacc
    29881 tgaacaagta gactggctta tgaagggctt ttctatcact ccaaaaatat agtagattga
    29941 aactagaata gtacacctct gcttctaaaa cattgttaga aatcgatttg actgtcctga
    30001 tcgatttgtc ctgttcttat ttcattttac tataaatcca tcagaaagtc gtgatttcta
    30061 ttgaaatgag gactttcttt ttatactcat ctgttttcaa aaagcattct agtccatctc
    30121 cgattaacga tggactttat cacctccttc tccagtcctt gtataacatc ttggagttga
    30181 ttcatgacat cttccaaagt ttgaaaggtt ttattcttaa atccacgttt acgaatctct
    30241 ttccacactt gttcaatggg gttcatctct ggtgtgtatg gaggaataaa tgcaaagcca
    30301 atattagtcg gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt
    30361 aaaagataat catctggata agcttgtgaa atctcctatt cctaaagccc ctttagcgca
    30421 taactttggc tcagcttcta ttatcgctca caccatccat cagaagttta atctgaaggt
    30481 acccaattat cgccaagaag aagattgggc taggatgggt ttaccaatca cacgtaagga
    30541 aatctctaat cggcatatca agacgagtca atactatttg gagccccttt ataacctctt
    30601 gcgagagaga ctattgactc agcccttact tcatgcggat gaaacttctt atagggtgct
    30661 agagagtgat agtcagctga cttactattg gacttttttg tctgggaaag ctgagaaaca
    30721 agggattacg ctttaccatc atgtcctaat cgatttgttc atatcttatt tcaatccatt
    30781 ataaatagtg agaaatatct atcctatctt ctagaatgtc ttccaaacgt ggaaactctc
    30841 gtaaacaaag aggttttaga ggcctattta ccatggacta aagttgtaca agaaaagtgc
    30901 aaataagaaa tctccagatt aggaactatc cgtgagttct ctagtctgga gatttttcaa
    30961 tagacttcgt tattggacgg ttacaattca taaagtgctg tagagagata acgttcaccg
    31021 ttatctggtg ctagggcaag gactttttta cctgtaccta attttttggc aacctcgatg
    31081 gctccgtaga tagctgcagc tgaggaaatc cctacaagga agccttcttt tccaccaatt
    31141 tcacgtccga gtgcaagagc gtcatctgat gttacacgaa cgataccatc ataggcttta
    31201 gtatcaagtg tatcaggaat aaatccagct gagatacctt gaattttgtg aggaccaggt
    31261 ttttcaccag atagaatagc agattcatct gcttctactg caaaaacttg aatgttagaa
    31321 ttttctgatt tgagtgcatg agaaacacca gaaatcgttc caccagtacc tactccagca
    31381 acaaaggcat ctaatccatc tttaccgaaa gcagctagta tctcagctcc tgttgttctt
    31441 tcgtgtactt ctggattagc tggattgtca aattgaagag gaaggaaacc atcacgttca
    31501 gcagcgattt cttgagcctt agcaatagca cctttcattc cctcgctacc aggagttagg
    31561 acgagttcag caccataagc ttggataatt ttacgtcgtt ctacactcat agtttcaggc
    31621 ataacgatga cgactttata ccctttagca gcacctaccc atgaaagtcc aataccggtg
    31681 tttccacttg ttgcttcaac aatagtagaa ccaagtttca gaataccatc ttgttcagct
    31741 ttttcaatca tgctaagggc aatacggtct tttacagatg aaccaggatt aaatgcttca
    31801 agctttatat agacgtctgc agcaccttct ggcacgatgt tgttaagttt aacaatcggt
    31861 gtttgaccga ttaattcagt aatgttgtta taaatagaca tatgaatacc tctttgtata
    31921 agatatcttt agtataacgc tatccatacc atttgtaaaa tatagaaatc ctatcgaagc
    31981 gataggattt ctatatatca tagatcaaag tcattaattt tcaagtgatt gcgttcagtt
    32041 aattctagta aataggtgtg aaggaaaacg atatcaggtt tcgtttgaat tgcttcattt
    32101 tctagtatat tttggtacgc atctttttat tttgctcgct aaagtagttg tgagaatgtc
    32161 aacgaaacgc tgtattattc attgtttaca ctaagtttac aggaacttca acctctcgta
    32221 aaccttggtc agttaaagtg acttttccat taaaaaactc cacaagtgca gctttaatag
    32281 tttctttttc ttctttatca acataaatca tcgtatcgac ttgatctgta aagtttgtat
    32341 ccagctccat gagatcatgt tctttaagga agttactgta ctcttggtac tgagcataag
    32401 acatttgaat agcaatgcca gcctgttctt ttatttcaat aataccaatt tctttgacag
    32461 ctaaggcgac actgccggcg taagcacgaa ttagtcctcc agcgcctagt ttaataccac
    32521 caaagtagcg tgtcacgacc acacagacat tggtgagatt gtgattttct agtaccccaa
    32581 gcatgggaac accagcagta ccactaggct caccatcatc acttgtacgt ttaatttcac
    32641 tacgttctcc aataataaag gcagagcagt tatgtgtcgc tttgtagtgt tcttttttga
    32701 tggcagtaat gaagtcacga gcctcttctt cgctataaac acgcttggca tggcagataa
    32761 agcgagattt tttgatttct tcttggactt ggccgtcctc tttaattgtc ctaaattcca
    32821 tgatttaatt gtactaaaaa atcatctaaa gtgctagttt ttacgaataa agaagtatga
    32881 aagtaaattt agattatctc ggtcgtttat ttactgagaa tgaattaaca gaagaagaac
    32941 gtcagttggc ggagaaactt ccagcaatga gaaaggagaa ggggaaactt ttctgtcaac
    33001 gttgtaatag cactattcta gcagaatggt atttgcccat cggtgcttac tattgtcgag
    33061 agtgcttgct gatgaagcga gtcagaagtg atcaaacttt atactatttt ccgcaggagg
    33121 attttccgaa gcaagatgtt ctcaaatggc gcggccaatt aactcctttt caagagaagg
    33181 tgtcagaggg actgcttcaa gcagtagaca agcaaaagcc aaccttagtt catgcggtaa
    33241 caggagctgg aaagacagaa atgatttatc aagtagtggc taaagtgatc aatgcgggtg
    33301 gtgcagtgtg tttggctagt cctcgcatag atgtttgttt ggagctgtac aagcgcctgc
    33361 aacaggattt ttcttgcggg atagctttgc tacatggaga atcggaacct tattttcgaa
    33421 caccactagt tgttgcaaca acccatcagt tattgaagtt ttatcaagct tttgatttgc
    33481 tgatagtgga tgaagtagat gcttttcctt atgttgataa tcccacgctt taccatgctg
    33541 tcaagaatag tgtaaaggag aatggattga gaatcttttt aacagcgact tcgaccaatg
    33601 agttagataa aaaggtccgt ttaggagaac taaaaagact gaatttaccg agacggtttc
    33661 atggaaatcc gttgattatt ccaaaaccaa tttggttatc ggattttaat cgctacttag
    33721 acaagaatcg tttgtcacca aagttaaagt cctatattga gaagcagaga aagacagctt
    33781 atccgttact catttttgct tcagaaatta agaaagggga gcagttagaa gaaatcttac
    33841 aggagcaatt tccaaatgag aaaattggct ttgtatcttc tgtaacagag gatcgattag
    33901 agcaagtaca agcttttcga gatggagaac tgacaatact tatcagtacg acaatcttgg
    33961 agcgtggagt taccttccct tgtgtggatg ttttcgtagt agaggccaat catcgtttgt
    34021 ttaccaagtc tagtttgatt cagattggtg gacgagttgg acgaagcatg gatagaccga
    34081 caggagattt gcttttcttc catgatgggt taaatgcttc aatcaagaag gcgattaagg
    34141 aaattcagat gatgaataag gaggctggtc tatgaagtgc ttgttatgtg ggcagactat
    34201 gaagactgtt ttaactttta gtagtctctt acttctgagg aatgatgact cttgtctttg
    34261 ttcagactgt gattctactt ttgaaagaat tggggaagag aactgtccaa attgtatgaa
    34321 aacagagttg tcaacaaagt gtcaagattg tcaactttgg tgtaaagaag gagttgaagt
    34381 cagtcataga gcgattttta cttacaatca agctatgaag gattttttca gtcggtataa
    34441 gtttgatgga gacttcctgt taagaaaagt tttcgcttca tttttaagtg aggagttgaa
    34501 aaagtacaaa gagtatcaat ttgttgtaat tcccctaagt cctgatagat atgctaatag
    34561 aggatttaat caggttgagg gcttggtaga ggcagcaggc tttgagtatc tggatttatt
    34621 agagaaaaga gaagagagag ccagttcttc taaaaatcgt tcagagcgct tggggacaga
    34681 acttcctttc tttattaaaa gtggagtcac tattcctaaa aaaatcctac ttatagatga
    34741 tatctatact acaggagcaa ctataaatcg tgttaagaaa ctgttggaag aagctggtgc
    34801 taaggatgta aaaacatttt cccttgtaag atgaggaaaa aatttgcaaa aatagaatga
    34861 aagcgttata ataaaatcat aaaaacaaaa atgtttagaa agaaggtact catatgatta
    34921 aatatagtat ccgtggtgaa aacctagaag taacagaagc aattcgtgat tatgtagttt
    34981 ctaaactcga aaagatcgaa aagtacttcc aaccagaaca agagttggat gcccgaatta
    35041 acttaaaagt ttatcgtgaa aaaacggcta aagtggaagt aacgattccg cttggatcta
    35101 ttactctccg cgcagaagat gtatctcaag atatgtatgg ttcaattgac cttgtaactg
    35161 ataaaattga acgtcagatt cgtaaaaata aaacaaaaat cgagcgtaaa aataaaaata
    35221 aggtagcaac tggtcaatta tttacagatg ctttggtgga agattcaaat attgtccagt
    35281 ctaaagttgt tcgttcaaaa caaattgatt taaaaccaat ggatttggaa gaagcaattc
    35341 tacaaatgga tttattgggg catgatttct ttatctatgt ggatgttgaa gatcagacaa
    35401 ccaatgtgat ttatcgtcgt gaggatggcg aaattggttt gttagaggtt aaagaatctt
    35461 aatttcaata tgtgtaaagg taggtttact gaattgtaaa ctaccttttt cttataattg
    35521 atagaattac tgattacact tttaaaaagt cgctttttga ggggtattat ggtataatgg
    35581 gtgccaagag gttttgaatg aaaaaatttt atgtaagtcc aatttttcct attctagtag
    35641 gattgattgc gtttggagtc ttatccactt tcattatttt tgttaataat aatctgttga
    35701 cggttttaat tttgtttctt tttgtaggag gctatgtttt tttatttaag aaactgagag
    35761 tgcattatac aaggagtgat gtagaacaga tacagtatgt aaaccaccaa gcggaagaaa
    35821 gtttgacagc tctattggaa cagatgcctg taggtgttat gaaattgaat ttatcttctg
    35881 gagaggttga gtggtttaat ccctatgctg aattgatttt gaccaaggaa gatggtgatt
    35941 ttgatttaga agctgttcaa acgattatca aggcttcagt aggaaatccg tctacttatg
    36001 ccaagcttgg tgagaagcgt tatgctgttc atatggatgc ttcttccggt gttttgtatt
    36061 ttgtagatgt atccagggaa caagccataa cagatgaatt ggtaacaagt agaccagtga
    36121 ttgggattgt ctctgtggat aattatgatg atttggagga tgcaacttct gagtcagata
    36181 ttagtcaaat caatagtttt gtagctaatt ttatatcaga gttttcagaa aaatacatga
    36241 tgttttctcg tcgggtaagt atggatcgat tttatctatt tactgactac acggtgcttg
    36301 agggcttgat gaatgataaa ttttctgtta ttgatgcttt cagagaagag tcgaaacaga
    36361 gacagttgcc cttgacctta agtatgggat tttcttatgg cgatggaaat catgatgaga
    36421 tagggaaagt tgctttgctc aatttgaact tggctgaagt acgtggtggc gaccaggtgg
    36481 ttgttaagga aaacgacgaa acgaaaaatc cagtttattt tggtggtggg tctgctgctt
    36541 caatcaagcg tacacggact cgtacgcgcg ctatgatgac agctatttca gataagattc
    36601 ggagtgtaga tcaggttttt gtagtcggtc acaaaaattt agacatggat gctttgggct
    36661 ctgctgtagg tatgcagttg ttcgccagca atgtgattga aaatagctat gctctttatg
    36721 atgaagaaca aatgtctcca gatattgaac gagctgtttc attcatagaa aaagaaggag
    36781 ttacgaagtt gttgtctgtt aaggatgcaa tggggatggt gaccaatcgt tctttgttga
    36841 ttcttgtaga ccattcaaag acagccttaa cattatcaaa agaattttat gatttattta
    36901 cccaaaccat tgttattgac caccatagaa gggatcagga ttttccagat aatgcggtta
    36961 ttacttatat cgaaagtggt gcaagtagtg ccagtgagtt ggtaacggaa ttgattcagt
    37021 tccagaattc taagaaaaat cgtttgagtc gtatgcaagc aagtgttttg atggctggta
    37081 tgatgttgga tactaaaaat ttcacctcgc gagtaactag tcggacattt gatgttgcta
    37141 gctatctcag aacgcgcgga agtgatagta ttgctatcca ggaaatcgct gcgacagatt
    37201 ttgaagaata tcgtgaggtc aatgaactta ttttacaggg gtgtaaatta ggttcagatg
    37261 tactaatagc agaggctaag gacacgaaat gctatgatac agttgttatt agtaaggcag
    37321 cagatgccat gttagccatg tcaggtattg aagcgagttt tgttcttgcg aagaatacac
    37381 aaggatttat ctctatctca gctcgaagtc gtagtaaact gaatgtacaa cggattatgg
    37441 aagagttagg cggtggaggc cactttaatt tggcagcagc tcaaattaaa gatgtaacct
    37501 tgtcagaagc aggtgaaaaa ctgacagaaa ttgtattaaa tgaaataaag gaaaaggaga
    37561 aagaagaatg aaagtaatct ttttagcaga tgttaaagga aaaggtaaaa aaggcgaaat
    37621 taaggaagta ccaacagggt atgcgcaaaa ctttcttatc aaaaagaatc tagccaaaga
    37681 agcgactgct caagctgtag gtgaacttcg tggtaaacaa aaatcggaag aaaaagctca
    37741 cgctgagatg attgcagaag gaaaagcaat taaagcacaa cttgaagcag aagaaactgt
    37801 tgtagaattt gttgaaaaag ttggtccaga tggtcgtacc tttggttcta ttaccaataa
    37861 gaagattgca gaagaattgc aaaagcaatt tggaattaag attgataaac gtcatattca
    37921 agtacaagct ccgattcgag cggttggttt gattgatgtg ccagtgaaaa tctatcaaga
    37981 tatcacaagt gtaatcaatc ttcgtgtgaa agaaggataa gtttacacct tcttgacaag
    38041 attgtaaaag gaagggaagt ctgatggcag aagtagaaga gttacgagta caacctcaag
    38101 atatcttagc tgagcaatcc gttttagggg ctatctttat tgatgagagt aaacttgttt
    38161 ttgtgcgaga atacattgag tctcgggact tttttaagta tgcccatcgt ttgattttcc
    38221 aagccatggt cgatttatcc gatcgtggtg atgccataga tgcaacaacg gttcgtacta
    38281 tccttgataa tcaaggtgat ttacagaata ttggtggctt gtcttacttg gttgagattg
    38341 tcaattctgt gccaacttct gctaatgcgg agtattatgc taagattgtt gcagaaaaag
    38401 caatgctacg tcgtttaatt gccaagttga cagagtctgt caaccaagct tacgaagcgt
    38461 cacaaccagc tgatgaaatt attgctcagg cagaaaaagg gctgattgat gtcagtgaaa
    38521 atgcaaatcg aagcgggttt aagaacattc gagatgtgtt gaatctcaac tttggaaatc
    38581 tggaagctcg ctcgcaacaa acgaccgata ttacaggtat tgcgacaggt tatcgtgatt
    38641 tggatcatat gacaacagga cttcatgagg aggagttgat tatcttagca gctcgtccag
    38701 cagttggtaa gacagcattt gccttgaata tcgctcagaa tattgggact aagttggaca
    38761 aaacggttgc tattttttca ctcgaaatgg gtgcggaaag cttggtagac cgtatgttag
    38821 ctgcagaagg cttggtggag tcacattcta tccgtacagg gcaattgaca gatgaggagt
    38881 ggcaaaaata tactattgct cagggtaatc tagctaacgc cagtatctat atcgatgata
    38941 cgccaggtat tcggattaca gagattcgtt ctcgttctcg taaattggct caagaaactg
    39001 gaaatcttgg tttgattttg atagactatt tgcaacttat cacgggaact ggtcgagaaa
    39061 atcgtcaaca agaagtttct gaaatttctc gtcagttgaa aatactagcc aaggaattga
    39121 aggttccagt aatcgctctg agtcagcttt ctcgtggtgt agaacaacgt caggacaaga
    39181 gaccggtctt gtctgatatt cgtgaatctg ggtctattga gcaggacgct gatatcgtag
    39241 cttttctcta tcgcgatgac tactatgaac gtggtggtga agaagaggag ggtatcccaa
    39301 ataataaggt ggaagttatt atcgagaaaa accgtagtgg agctcgtgga acagtggaat
    39361 tgattttcca aaaagaatac aataaatttt caagtatctc aaagagggag gcataagatg
    39421 tcagatgcat ttacagatgt agccaagatg aaaaaaatca aagaagaaat caaggcacat
    39481 gagggacaag tcgtagaaat gactttggag aatggtcgta agcgccaaaa aaatagattg
    39541 ggtaagctaa ttgaagttta tccatcccta tttattgtgg agtttgggga tgtggaagga
    39601 gataaacaag ttaatgttta cgttgaatcc tttacttact cagatattct tacagaaaag
    39661 aatttgattc attatcttga ctaaagtgag aaattttctc actttttctt ttttctccga
    39721 ataatttagg tgaaggcaat catcgcttta tattattttt taaggaggaa gaatgaaaat
    39781 tttaccgttt atagcaagag gaacaagtta ttacttgaag atgtcagtta aaaagcttgt
    39841 tcctttttta gtagtaggat tgatgctagc agctggtgat agtgtctatg cctattccag
    39901 aggaaatgga tcgattgcgc gtggggatga ttatcctgct tattataaaa atgggagcca
    39961 ggagattgat cagtggcgca tgtattctcg tcagtgtact tcttttgtag cctttcgttt
    40021 gagtaatgtc aatggttttg aaattccggc agcttatgga aatgcgaatg aatggggaca
    40081 tcgtgctcgt cgggaaggtt atcgtgtaga taatacaccg acgattggtt ccattacttg
    40141 gtctactgca ggaacttatg gtcatgttgc ctgggtgtca aatgtaatgg gagatcagat
    40201 tgagattgag gaatataact atggttatac agaatcctat aataaacgaa ttataaaagc
    40261 aaacacgatg acaggattta ttcattttaa agatttggat ggtggcagtg ttgggaatag
    40321 tcaatcctca acttcaacag gcggaactca ttattttaag accaagtctg ctattaaaac
    40381 tgaaccccta gttagtgcaa ctgtgattga ttactattat cctggagaga aggttcatta
    40441 tgatcaaatt ctcgaaaaag acggctacaa gtggttgagt tatactgcct ataatggaag
    40501 ctatcgttat gttcaattgg aggctgtgaa taaaaatcct ctaggtaatt ctgttctttc
    40561 ttcaacaggt ggaactcatt attttaagac caagtctgct atcaaaactg aacccctagt
    40621 tagtgcaact gtgattgatt actattatcc tggggagaag gttcattatg atcagatact
    40681 tgaaaaagac ggctacaagt ggttgagtta tacggcttat aacggaagtc gtcgctatat
    40741 acagctagag ggagtgactt cttcacaaaa ttatcagaat caatcaggaa atatctctag
    40801 ctatggatcc aataatagtt caactgtcgg ttggaagaaa ataaatggta gttggtatca
    40861 tttcaaatca aatggttcta aatcaacagg atggctgaaa gatggttcta gctggtatta
    40921 tttgaaatta tctggtgaaa tgcagacagg atggttaaag gagaatggct cgtggtatta
    40981 tctgggtagt tcaggggcaa tgaaaacagg ctggtaccag gtctctggta agtggtatta
    41041 ttcttactct tcaggcgcct tagctgttaa tacgacggtg gatggctaca gagtaaacag
    41101 tgatggagaa cgagtataga aaattggagt aggagaaatt tcctgctctt ttctgtaagc
    41161 agtttccttg acatttttct tattttgcgc tatcatatat acatataata tatgggagtc
    41221 tgtgtacagt ctgagaggaa gtgttaaact tcgaccgcac ctgatctggg taatgccagc
    41281 ggagggaacg atacttagtc taattttgca ccttttccat gtatggtaaa ggtttttctt
    41341 tttttaaaaa ggaaaacgag aagaggaggt tcttatgaaa gcaagcattg ccttgcaagt
    41401 tttaccccta gcacagggga ttgatcggat agctgttatt gatcaggtca ttgcttatct
    41461 gcaaactcaa gaagtgacga tggtagtgac accatttgaa acggtcttgg aaggggagtt
    41521 tgatgagctt atgcgcattc taaaagaagc gctggaagtg gcagggcagg aggcagacaa
    41581 tgtctttgcc aatgtcaaaa taaatgtagg agagatttta agtattgatg agaaacttga
    41641 gaagtatact gagacgccac attagtctat tgggctttct cggagtcttg tcaatctggc
    41701 agttagcagg ttttcttaaa cttctcccca agtttatcct gccgacacct cttgaaattc
    41761 tccagccctt tgttcgtgac agagaatttc tctggcacca tagctgggcg accttgagag
    41821 tggctttact ggggctgatt ttgggagttt tgattgcctg tcttatggct gtgctcatgg
    41881 atagtttgac ttggctcaat gacctgattt accctatgat ggtggttatt cagaccattc
    41941 cgaccattgc catagctcct atcctggtct tgtggctagg ttatgggatt ttgcccaaga
    42001 ttgtcttgat tatcttaacg acaacttttc ccatcatcgt cagcattttg gacggtttta
    42061 ggcattgcga caaggatatg ctgaccttgt ttagtctgat gcgggccaag ccttggcaaa
    42121 tcctgtggca ttttaaaatt ccagtcagcc tgccctactt ttatgctggt ctgagggtca
    42181 gtgtctccta cgcttttatc acaacagtgg tatctgagtg gttgggaggt tttgaaggtc
    42241 ttggtgttta tatgattcag tctaaaaaac tgtttcagta tgataccatg tttgccatta
    42301 ttattctggt gtcgattatc agtcttttgg gtatgaagct ggtcgatatc agtgaaaaat
    42361 atgtgattaa atggaaacgt acttaaaata gagcgttttt gaaaaagaaa agaggaaatc
    42421 aaaatgaaga aaacatggaa agtgttttta acgcttgtaa cagctcttgt agctgttgtg
    42481 cttgtggcct gtggtcaagg aactgcttct aaagacaaca aagaggcaga acttaagaag
    42541 gttgacttta tcctagactg gacaccaaat accaaccaca cagggcttta tgttgccaag
    42601 gaaaaaggtt atttcaaaga agctggagtg gatgttgatt tgaaattgcc accagaagaa
    42661 agttcttctg acttggttat caacggaaag gcaccatttg cagtgtattt ccaagactac
    42721 atggctaaga aattggaaaa aggagcagga atcactgccg ttgcagctat tgttgaacac
    42781 aatacatcag gaatcatctc tcgtaaatct gataatgtaa gcagtccaaa agacttggtt
    42841 ggtaagaaat atgggacatg gaatgaccca actgaacttg ctatgttgaa aaccttggta
    42901 gaatctcaag gtggagactt tgagaaggtt gaaaaagtac caaataacga ctcaaactca
    42961 atcacaccga ttgccaatgg cgtctttgat actgcttgga tttactacgg ttgggatggt
    43021 atccttgcta aatctcaagg tgtagatgct aacttcatgt acttgaaaga ctatgtcaag
    43081 gagtttgact actattcacc agttatcatc gcaaacaacg actatctgaa agataacaaa
    43141 gaagaagctc gcaaagtcat ccaagccatc aaaaaaggct accaatatgc catggaacat
    43201 ccagaagaag ctgcagatat tctcatcaag aatgcacctg aactcaagga aaaacgtgac
    43261 tttgtcatcg aatctcaaaa atacttgtca aaagaatacg caagcgacaa ggaaaaatgg
    43321 ggtcaatttg acgcagctcg ctggaatgct ttctataaat gggataaaga aaatggtatc
    43381 cttaaagaag acttgacaga caaaggcttc accaacgaat ttgtgaaata atgacagaaa
    43441 ttagactaga gcacgtcagt tatgcctatg gtcaggagag gattttagag gatatcaacc
    43501 tacaggtgac ttcaggcgaa gtggtttcca tcctaggccc aagtggtgtt ggaaagacca
    43561 ccctctttaa tctaatcgct gggattttag aagttcagtc agggagaatt gtccttgatg
    43621 gtgaagaaaa tcccaagggg cgcgtgagtt atatgttgca aaaggatctg ctcttggagc
    43681 acaagacggt gcttggaaat atcattctgc ccctcttgat tcaaaaggtg gataaggcag
    43741 aagctatttc ccgagcggat aaaattcttg cgaccttcca gctgacagct gtaagagaca
    43801 agtatcctca tgaacttagc ggtgggatgc gccagcgtgt agccttactc cggacctacc
    43861 tttttgggca caagctcttt ctcttagatg aggcctttag cgccttggat gagatgacaa
    43921 agatggaact ccacgcttgg tatcttgaga ttcacaaaca gttgcagcta acaactctga
    43981 tcatcacgca tagtattgag gaggccctca atctcagcga ccgcatctat atcttgaaaa
    44041 atcgccctgg gcagattgtt tcagaaatta aactagattg gtctgaagat gaggacaagg
    44101 aagtccaaaa gattgcctac aaacgtcaaa tcttggcaga attaggctta gataagtaga
    44161 aaaataggga gttggtgaag attatccttt accagcgccc tttttctttt aaaaatgaga
    44221 aaatttcggt ataatagtca aacaaggtca aggtttaaag agagaggtgg gtttgttatg
    44281 agatttaaaa atacatcgga tcatattgag gcctacatca aggcgatttt agatcaatct
    44341 ggtatcgtgg agttgcaacg gagtcagttg gcagatacct ttcaggttgt tcctagtcag
    44401 attaactacg tgatcaagac acgctttacg gaaagtagag gctacttggt tgaaagtaag
    44461 cgtggtggcg gaggctacat tcgtatagga cggattgagt tttctagtca tcatgaaatg
    44521 ctccgggatc tgctttactc gattggtgag cgagtcagtc aagaaattta tgaggatatt
    44581 ctccagcttt tggttgagca ggaattgatg accaagcagg agatgaattt gctagaatca
    44641 gtagctttgg atcgcgtttt aggagaagaa gctccagttg ttcgagcaaa catgctacgt
    44701 caggtcatac aagaggtaga tagaaaaggg aagtaagatg aactattcaa aagcattgaa
    44761 tgaatgtatc gaaagtgcct acatggttgc tggacatttt ggagctcgtt atctagagtc
    44821 gtggcacttg ttgattgcca tgtctaatca cagttatagt gtagcagggg caactttaaa
    44881 tgattatccg tatgagatgg accgtttaga agaggtggct ttggaactga ctgaaacgga
    44941 ctatagccag gatgaaacct ttacggaatt gccgttctcc cgtcgtttgc aggttctttt
    45001 tgatgaagca gagtatgtag cgtcagtggt ccatgctaag gtactaggga cagagcacgt
    45061 cctctatgcg attttgcatg atggcaatgc cttggcgact cgtatcttgg agagggctgg
    45121 tttttcttat gaagacaaga aagatcaggt caagattgct gcccttcgtc gaaatttaga
    45181 agaacgtgca ggctggactc gtgaagatct caaggcttta cgccaacgcc atcgtacagt
    45241 agctgacaag caaaattcta tggccaatat gatgggcatg ccgcagactc ctagtggtgg
    45301 tctcgaggac tatacgcatg atttgacaga gcaagcgcgt tctggcaagt tagaaccagt
    45361 catcggtcgg gacaaggaaa tctcgcgtat gattcaaata ttgagccgta agactaagaa
    45421 caaccctgtc ttggttgggg atgctggtgt cgggaaaaca gctctggccc ttggtcttgc
    45481 ccagcgtatt gctagtggtg acgtgcctgc ggaaatggct aagatgcgcg tgttagaact
    45541 tgatttgatg aatgtcgttg cagggacacg cttccgtggt gactttgaag aacgcatgaa
    45601 taatatcatc aaggatattg aagaagatgg ccaagtcatc ctctttatcg atgaactcca
    45661 caccatcatg ggttctggta gcgggattga ttcgactctg gatgcggcca atatcttgaa
    45721 accagccttg gcgcgtggaa ctttgagaac ggttggtgcc accactcagg aagaatatca
    45781 aaaacatatc gaaaaagatg cggctctttc tcgtcgtttc gctaaagtga cgattgaaga
    45841 accaagtgtg gcagatagta tgactatttt acaaggtttg aaggcgactt atgagaaaca
    45901 tcaccgtgta caaatcacag atgaagcggt tgaaacagcg gttaagatgg ctcatcgtta
    45961 tttaaccagt cgtcacttgc cagactctgc tatcgacctc ttggacgaag cagcagcaac
    46021 agtgcaaaat aaggcaaagt atgtaaaagc agacgattca gatttgagtc cagctgacaa
    46081 ggccctgatg gatggcaagt ggaaacaggc agcccagcta atcgcaaaag aagaggaagt
    46141 acctgtctac aaagacttgg tgacagagtc tgatattttg accaccttga gtcgcttgtc
    46201 aggaatccca gttcaaaaac tgactcagac tgacgctaag aaatatctaa atcttgaagc
    46261 agaactccat aaacgggtta tcggtcaaga tcaagctgtt tcaagtatta gtcgtgcgat
    46321 tcgccgtaat cagtcaggaa ttcgcagtca caagcgtccg atcggttcct ttatgttcct
    46381 aggacccaca ggtgtcggta agaccgagtt ggccaaggct ttggcagaag ttctctttga
    46441 tgacgaatca gcccttatcc gctttgatat gagtgagtat atggagaaat tcgcagccag
    46501 ccgtctaaat ggagctcctc caggctatgt gggttacgaa gaaggtgggg agttgacaga
    46561 gaaggttcgc aataaaccct attccgttct cctctttgat gaggtagaga aggctcaccc
    46621 agatatcttt aatgttctct tgcaggttct ggatgacggt gtcttgacag atagcaaggg
    46681 acgcaaggtc gatttttcaa ataccattat cattatgaca tcgaacctag gtgcgactgc
    46741 ccttcgtgat gataagactg ttggttttgg ggctaaggat attcgttttg accaggaaaa
    46801 tatggaaaaa cgcatgtttg aagaactgaa aaaagcttat agaccggaat tcatcaaccg
    46861 tattgatgag aaggtggtct tccatagcct atctagtgac catatgcagg aagtggtgaa
    46921 gattatggtc aagcctttag tggcaagttt ggctgaaaaa ggcattgact tgaaattaca
    46981 agcttcagct ctgaaattgt tagcaaatca aggatatgac ccagagatgg gagctcgccc
    47041 acttcgcaga accctgcaaa cagaagtgga ggacaagttg gcagaacttc ttctcaaggg
    47101 agatttagtg gcaggcagca cacttaagat tggtgtcaaa gcaggccagt taaaatttga
    47161 tattgcataa aagaataaaa gtatcagcat ctgaccataa gtcacagtgg agtgaaactc
    47221 aatgaaaatc aaagaacaaa ctaggcagct agccgcaggt tgctcaaaac actgttttga
    47281 ggttgtagat agagctgacg tggtttggag agattttcga agagtataag ctgcaagatg
    47341 aatgattttc ttgtattgac gttgttgttg acaaaaagta gcggataaat gaaatccatt
    47401 ccattatcat agatgatagg ctggtaggaa attttcaaat agcatacagg aaatagatgt
    47461 atggagttct ggtagtagaa agggagagag atgaacattt tagttgcaga tgacgaggaa
    47521 atgattagag aaggaattgc agcatttctg acagaagagg gttatcatgt cattatggct
    47581 aaggatggac aagaggtctt ggaaaaattt caagatctcc ctatccatct catggtattg
    47641 gatttaatga tgcctaggaa gagtggtttt gaagtgttaa aagaaatcaa tcaaaagcac
    47701 gatattcctg tcatcgtctt gagtgctctg ggagatgaaa ctactcagtc acaggtattt
    47761 gatctctatg ctgatgatca tgtgacaaaa cctttttctt tggtactgct tgtcaagcgt
    47821 attaaggcgc ttatcagacg ttactacgtc atagaggata tttggcgata tcaggatgta
    47881 acagtggatt ttacctctta caaagcacat tataaaaatg aagaaattga tctcaaacca
    47941 aaggaattac tggtactaaa gtgtttgatt cagcataaaa atcaagtttt aagtagagag
    48001 cagatattgg aagaaatttc aaaagatgta gctgatttac cttgtgatag ggtcgttgat
    48061 gtctatattc gtactcttcg caaaaaatta gctttagatt gtatcgtgac tgtgaaaaat
    48121 gttgggtata agattagctt atgataaaaa atcctaaatt attaaccaag tcttttttaa
    48181 gaagttttgc aattctaggt ggtgttggtc tagtcattca tatagctatt tatttgacct
    48241 ttccttttta ttatattcaa ctggaggggg aaaagtttaa tgagagcgca agagtgttta
    48301 cggagtattt aaagactaag acatctgatg aaattccaag cttactccag tcttattcaa
    48361 agtccttgac catatctgct caccttaaaa gagatattgt agataagcgg ctccctcttg
    48421 tgcatgactt ggatattaaa gatggaaagc tatcaaatta tatcgtgatg ttagatatgt
    48481 ctgttagtac agcagatggt aaacaggtaa cagtgcaatt tgttcacggg gtggatgtct
    48541 acaaagaagc aaagaatatt ttgcttttgt atctcccata tacatttttg gttacaattg
    48601 ctttttcctt tgttttttct tatttttata ctaaacgctt gctcaatcct cttttttaca
    48661 tttcagaagt gactagtaaa atgcaagatt tggatgacaa tattcgtttt gatgaaagta
    48721 ggaaagatga agttggtgaa gttggaaaac agattaatgg tatgtatgag cacttgttga
    48781 aggttattca tgagttggaa agtcgtaatg agcaaattgt aaaattgcaa aatcaaaagg
    48841 tttcctttgt ccgcggagca tcacatgagt tgaaaacccc tttagccagt cttagaatta
    48901 tcctagagaa tatgcagcat aatattggag attacaaaga tcatccaaaa tatattgcaa
    48961 agagtataaa taagattgac cagatgagcc acttattgga agaagtactg gagtcttcta
    49021 aattccaaga gtggacagag tgtcgtgaga ccttgactgt taagccagtt ttagtagata
    49081 ttttatcacg ttatcaagaa ttagctcatt caataggtgt tacaattgaa aatcaattga
    49141 cagatgctac cagggtcgtc atgagtctta gggcattgga taaggttttg acaaacctga
    49201 ttagtaatgc aattaaatat tcagataaaa atgggcgcgt aatcatatcc gagcaagatg
    49261 gctatctctc tatcaagaat acatgtgcgc ctctaagtga ccaagaacta gaacatttat
    49321 ttgatatatt ctatcattct caaatcgtga cagataagga tgaaagttcc ggtttgggtc
    49381 tttacattgt gaataatatt ttagaaagct atcaaatgga ttatagtttt ctcccttatg
    49441 aacacggtat ggaatttaag attagcttgt agacagatta gttttttatt aaagttcata
    49501 tagggttaac ataagtgtgt tattctttgt gtagataaaa gaaaggatac taatatggta
    49561 ttagcgatta ttttagtaac attctttatt cgattgattt ttttaaagcg ttcgatagag
    49621 aatgagaaac gaatccttag caatggcggg aaagaatttg gagttgagaa tacaaaacga
    49681 ttaactatgg ctcatattgt tttttatctc tcttgcttgg ttgaggcaat ggtgcacaag
    49741 acaatttttg atggcatggg catggttggt ttagtcttgc ttattttttc tatgctgatg
    49801 ttgatgttgg tgattcactt gttgggagat atttggacag tgaagcttat gcttgtcaat
    49861 aatcacaaat atgtagatca tatcttgttt aggacagtaa aacatcctaa ttacttttta
    49921 aatattcttc ctgagttgat tggcttgacc ttgttgagtc atgcttatgt gacttttgtt
    49981 ttagtttttc cagtttatgc agttattttg tatcgacgaa tagctgaaga ggaaaagcta
    50041 ttacatgaag ttataatccc aaatggaagc ataaagagat aaatacaaaa ttcgatttat
    50101 atacagttca tattgaagtg atatagtaag gttaaagaaa aaatatagaa ggaaataatt
    50161 atgtttgcat caaaaagcga aagaaaagta cattattcaa ttcgtaaatt tagtgttgga
    50221 gtagctagtg tagtagttgc tagcttgttc ttaggaggag tagtccatgc agaaggggtt
    50281 agaagtgggg atacccccaa ggttacatct agtgggcaag atatatcgaa gaagtatgct
    50341 gatgaagtca agtcgcatct agaaaaaata ttgagtgaga tccaattaga taaaagaaaa
    50401 catacccaaa atctcgcctt caacaaaaag ttgagcagaa ttcaaacgga gtatttctat
    50461 ttaaaaaaga agttgaaagc tgagttgacg tcaaaaacag aagagttgac gtcaaaaaca
    50521 aaaaaagagt tagacgcagc ttttgagcag tttaaaaaag atacattatc aacagaacca
    50581 gaaaaaaagg ttgcagaagc tgagaagaag gtagcagaag ctaagaaaaa agccgaggat
    50641 caaaaagaag aagatcgtcg taactaccca accaatactt acaaaacgct tgaacttgaa
    50701 attgctgagt tcgatgtgaa agttaaagaa gcggagcttg aactattaaa agagaaagct
    50761 aaggaatctc gagacgagga aaaaattaag caagcagaag cggaagttga gagtaaacaa
    50821 gctgaggcta caaggttaga aaacatcaag acagatcgta aaaaagcaga agaagaagct
    50881 aaacgaaaag cagatgctaa gttgaaggaa gctaatgtag cgacttcaga tcaaggtaaa
    50941 ccaaaggggc gggcaaaacg aggagttcct ggagagctag caacacctga taaaaaagaa
    51001 aatgatgcga agtcttcaga ttctagcgta ggtgaagaaa ctcttccaag ctcatccctg
    51061 aaatcaggaa aaaaggtagc agaagctgag aagaaggttg aagaagctga gaaaaaagcc
    51121 aaggatcaaa aagaagaaga tcgccgtaac tacccaacca atacttacaa aacgcttgaa
    51181 cttgaaattg ctgagtccga tgtgaaagtt aaagaagcgg agcttgaact agtaaaagag
    51241 gaagctaagg aacctcgaga cgaggaaaaa attaagcaag caaaagcgga agttgagagt
    51301 aaacaagctg aggctacaag gttagaaaaa atcaagacag atcgtaaaaa agcagaagaa
    51361 gaagctaaac gaaaagcagc agaagaagat aaagttaaag aaaaaccagc tgaacaacca
    51421 caaccagcgc cggctcctca accagaaaaa ccagctccaa aaccagaaaa accagctcca
    51481 aaaccagaaa aaccagctcc agctccaaaa ccagagaatc cagctgaaca accacaacca
    51541 gcgccggctc ctcaaccaga aaaaccagct ccaaaaccag aaaaaccagc tccagctcca
    51601 aaaccagaga atccagctga acaaccaaaa gcagaaaaac cagcacaacc atctactcca
    51661 aaaacaggct ggaaacaaga aaacggtatg tggtacttct acaatactga tggttcaatg
    51721 gcgacaggat ggctccaaaa caatggctca tggtactacc tcaacagcaa tggcgctatg
    51781 gcgacaggat ggctccaaaa caatggttca tggtactatc taaacgctaa tggttcaatg
    51841 gcaacaggat ggctccaaaa caatggttca tggtactacc tcaacgctaa tggttcaatg
    51901 gcgacaggat ggctccaaaa caatggttca tggtactacc tcaacgctaa tggtgatatg
    51961 gcgacaggat ggctccaaaa caatggctca tggtactacc taaacgctaa tggtgatatg
    52021 gcgacaggat ggctccaaaa caacggttca tggtactacc taaacagcaa tggtgctatg
    52081 gtaacaggat ggctccaaaa caatggctca tggtattacc tcaacgctaa tggtgatatg
    52141 gcgacaggtt gggtgaaaga tggagacacc tggtactatc ttgaagcatc aggtgctatg
    52201 aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg ctcaggtgcc
    52261 cttgcagtca acacaactgt agatggctat ggagtcaatg ccaatggtga atgggtaaac
    52321 taaacctaat ataactagtt aatactgact tcctgtaaga actttttaaa gtattcccta
    52381 caaataccat atcctttcag tagataatat acccttgtag gaagtttaga ttaaaaaata
    52441 actctgtaat ctctagccgg atttatagcg ctagagacta cggagttttt ttgatgagga
    52501 aagaatggcg gcattcaaga gactctttaa gagagttacg ggttttaaac tattaagcct
    52561 tctccaattg caagagggct tcaatctctg ctagggtgct agcttgcgaa atggctccac
    52621 ggagtttggc agcgccagat gttccacgga gatagtgagg agcgaggccg cggaattcac
    52681 gaactgcgac gttttctcct ttgaggttaa tcaatcgttt caagtgttcg taggcgatct
    52741 tcatcttgtc ttcaaaggtc aaatcaggta ggatttctcc tgtttcaaag taatggttga
    52801 tttggttgaa gaggtaagga tttcccatgg cagctcggcc aatcatgact gcgtcagcac
    52861 caacttcttc gatgcgttgc ttggcttctt ggacagtacg gatatcaccg ttggcgatga
    52921 atggaatctt ggttagagct tgggcaacct tgtaaagggt ctcaaggtct gcgtggccag
    52981 tatacatttg ttcacgggta cggccatgca tggcgagggc agaaacacct gcagcttcag
    53041 cagcgagggc attttctact gccagagatg ggtccgccca gccggtacgc attttgacag
    53101 taagtgggat atcaaggaca gactggacct tgttgatgat ggagtaaatc ttgtctggat
    53161 ccttgagcca catagcacca gcttcgttct tcacgatttt gttgacaggg cagcccatgt
    53221 tgatatcgac gatatcggtc ttggtgtttt cttggatgaa ttctgctgcg cgtgctaggc
    53281 tgtcttcatc gctaccaaaa agttggatag agacagggtt ttcgccctca tcgatatgaa
    53341 gcatgtgcag ggttttttcg ttgttgtatt ggattccctt gtcagagacc atttccatta
    53401 caacgagtcc agctccgagc tcttttgcga tggtacgaaa ggctgagttg gtcacgccag
    53461 ccataggcgc taaaacggta cgattgggaa tctcaacatt gccaatcata aaaggtgtat
    53521 taagatttgt cacgaatgag ttcctccagg tccttttcat caaagttgta agtagtttgg
    53581 cagaattgac aagtgatttc tgccccgtgg tcttcttctt tcatttcctg taagtctgag
    53641 cttggaaggc tggcaagagc gttcataaag cgttcatggc tacagtcaca ttggaaacgg
    53701 atttcttctt cagaaagacg cttgtaggct tcgtccccgt agatagcctt gaggagggct
    53761 tcgatatggt cgtcgctttc gagaagagta gagatagctg gcatttcttg gatgcgtttt
    53821 tcaaagcgag caatctcttc tttcttggct cctggcaaga cttgaactag gaaaccacct
    53881 gcaaccttga ccttgtcttc ctcgtccaaa aggacattga ggccgaccgc tgaaggcgtt
    53941 tgttggcttt cagtaaggta aaaggcaagg tcttcaccga tttctccaga gatgagggga
    54001 gttatagagt tgtaaggatt tccagtaccg tagtctgtga taacgaggaa ttgaccattt
    54061 ccaacaaaag gtccgactag gacttcacca gtcgcagtct ttttgatgtc aacaccagga
    54121 ttttgaacgt agcctttgac gttccccttg gtatcagcga cggtgataat agcacctaga
    54181 gagctagatc ccaacacctt aactgtaagt ttggtatttc ctttttcatt ggctgcgaga
    54241 atctggctgg cgataagagt tcgaccaagc gctacagttg agctagcttg ggtttgatgt
    54301 ttttcttgag cagtgcggac ggtttcagtg ctatcaagga caaaagcacg aaaggctccg
    54361 ctttctgata tagttttaat aattttatcc atagctacta ttttagcata aaaatgccca
    54421 aagggggagc cgtgtgttta ctgattttca ggataatgga ccaggaaatc agcatgaaaa
    54481 taaaaagaga aacagattat tttagcattt gtaagattta tgctatgctt aaggtagaaa
    54541 atgaaagggg taacaaatgt atttaggaga tttgatggag aaagccgagt gtggtcaatt
    54601 ttcaatcctt tcctttctat tacaagagtc tcagacgacc gtcaaggctg taatggaaga
    54661 aacaggattt tcaaaagcaa ccctaaccaa atatgtcacc ctgctcaatg acaaggcttt
    54721 ggatagtggc ttagagctgg ctattcactc agaagatgaa aatctgcgtc tgtctatcgg
    54781 tgcagctacc aaggggagag atattcggag cttgtttttg gagagtgctg ttaaatacca
    54841 gattttggtt tatcttctct accaccaaca gtttttagcc catcagctgg ctcaagaatt
    54901 ggtgattagc gaggctacgc ttggtcgtca cttggctggt ttaaatcaga ttttgtcaga
    54961 atttgattta tccatccaaa atggacgttg gcgaggtcca gagcatcaga ttcgctattt
    55021 ctatttctgt cttttccgaa aggtctggtc gagtcaggaa tgggaaggtc acatgcagaa
    55081 accagagaga aaacaggaga ttgccaattt agaggaaatc tgcggtgcaa gtttgtctgt
    55141 ggggcagaaa ttggacttgg ttctctgggc tcacatcagt caacaacgtc ttcgggtcaa
    55201 tgcttgtcag tttcaagtca tagaagagaa aatgcgaggg tattttgaca atatcttcta
    55261 tcttcgtttg ctgagaaagg ttccgtcctt ttttgctggg caacatattc cactaggagt
    55321 tgaggatggt gagatgatga tattcttctc ttttctccta tctcatcgca ttcttcctct
    55381 tcatactatg gaatatattc ttggttttgg agggcagttg gcagatttac tgacgcaatt
    55441 gattcaagaa atgaagaagg aggaactatt gggggattat acagaggacc atgtcaccta
    55501 tgaactgagt cagctttgtg ctcaagtcta tctctataag ggctatattt tacaggatcg
    55561 ctacaagtac cagttagaga atcgtcatcc atatttactg atggaacatg attttaaaga
    55621 gacagcagag gagatttttc atgctctacc tgcttttcaa caggggacag atttagataa
    55681 gaagattctc tgggaatggc tccagttaat cgaatatatg gctgaaaacg gtggccagca
    55741 tatgcggatt ggtctggatt tgacatctgg ttttcttgtc ttttcaagga tggcagccat
    55801 tttgaaacgg tatttggaat acaatcgttt tattaccatt gaagcttatg accctagtcg
    55861 gcattatgat ttgctggtta ccaataaccc gattcataag aaggaacaga ccccagtcta
    55921 ttatttaaaa aatgacttgg atatggaaga tttggcaggg attcgtcagt tattattcac
    55981 ttaaaaggct tggttaatcc aggtcttttt tgtgaaattc acacaatctc ctcacatttt
    56041 tttaaaaatt aaaaaaagtt gataaacaag aaagcgcttt attttgtata ctagtaagtg
    56101 taaagaggaa acacctcaag atctttatca ggaggacagt acatgtcaca agaaaaatac
    56161 atcatggcca ttgaccaggg aactacaagt tctcgtgcca tcattttcaa caaaaaaggg
    56221 gaaaaggtta gctcgagtca aaaagagttt acccagattt tccctcaggc aggttgggtt
    56281 gagcacaatg ccaatgaaat ttggaactct gttcagtcag ttattgcggg tgctttcatc
    56341 gaaagtggtg tcaagccaaa tcaaatcgag gcaatcggga ttaccaacca acgtgaaaca
    56401 acggttgtct gggataagaa aacaggactt cctatctaca atgctatcgt ttggcagtca
    56461 cgccagacag cacctttggc tgagcaacta aaaagccaag gttatgtgga aaaattccat
    56521 gaaaagactg gtttgattat tgatgcttac ttctctgcta ccaaggttcg ttggattttg
    56581 gatcatgtag aaggcgctca agagcgagca gaaaaagggg aattgctctt tggtactatc
    56641 gatacttggt tggtttggaa attgactgac ggtgcggctc acgtgactga ctactcaaat
    56701 gcagctcgta ccatgcttta taacattaaa gaactcaaat gggatgatga gattttggaa
    56761 atccttaaca ttccgaaggc tatacttcca gaagttcgtt ctaactccga aatctacggc
    56821 aagacagctc cattccattt ctacggtgga gaggtgccaa tctcaggtat ggctggggac
    56881 caacaagcag ccctctttgg acagttggct tttgagccag gtatggttaa gaatacttat
    56941 ggaacaggct ctttcatcat catgaatact ggggaagaga tgcagttgtc tgaaaacaac
    57001 ctcttgacaa ccattggtta cggaatcaac ggtaaggttt attatgcctt ggaaggttct
    57061 atcttcatcg caggaagtgc tattcagtgg cttcgtgacg gtcttcgcat ggttgaaaat
    57121 tcaccagaat ctgaaaaata cgctcgtgat tctcacaaca acgatgaagt ttatgtcgtt
    57181 ccagccttta caggtctagg cgctccatac tggaaccaaa atgctcgtgg ttccgtcttt
    57241 ggtttgactc gtggaacaag caaagaagac tttatcaagg cgactttgca atctattgct
    57301 tatcaagtgc gtgacatcat cgacaccatg caagtggata ctcagactgc cattcaagta
    57361 ttgaaggtgg atggtggtgc agccatgaac aacttcctca tgcagttcca ggcggatatt
    57421 ttaggcattg atattgcacg tgctaaaaac ttggaaacaa cagctctagg agcggccttc
    57481 ctagcaggtt tatcagtagg gtactggaaa gacttggacg agttgaaact cttgaacgag
    57541 acaggagaac tctttgagcc atctatgaat gaatctcgca aggaacaact ctacaagggc
    57601 tggaagaagg ctgtgaaagc aactcaagtc tttgcggaag tagacgacta atactggcag
    57661 aataaagcga tttatttaga aagtgtgtaa atatggaatt ttcaaagaaa acacgtgaat
    57721 tgtcaattaa aaaaatgcag gaacgcaccc tggacctctt gattatcggt ggaggaatca
    57781 caggagctgg tgtagccttg caggcggcag ctagcggtct tgagactggt ttgattgaaa
    57841 tgcaagactt tgcagaagga acatctagtc gttcaacaaa attggttcac ggaggtcttc
    57901 gctacctcaa acaattcgac gtagaagtgg tctcagatac ggtttctgaa cgtgcagtgg
    57961 ttcaacaaat cgctccacac attccaaaac cagatccaat gctcttacca gtttacgatg
    58021 aagatggagc aacctttagc ctcttccgtc ttaaagtagc catggacttg tacgacctct
    58081 tggcaggtgt tagcaacaca ccaactgcga acaaggtttt gagcaagaaa gaagtcttgg
    58141 aacgccagcc aaacttgaag aaggaaggct tggtaggagg tggagtgtat cttgacttcc
    58201 gtaacaacga tgcgcgtctc gtgattgaaa acatcaaacg tgccaaccaa gacggtgccc
    58261 tcattgccaa ccacgtgaag gcagaaggct tcctctttga cgaaagtggc aagattacag
    58321 gtgttgtagc tcgtgatctc ttgacagacc aagtatttga aatcaaggcc cgtctggtta
    58381 ttaatacaac aggtccttgg agtgataaag tacgtaattt gtctaataag ggaacgcaat
    58441 tctcacaaat gcgcccaact aagggagttc acttggtagt ggattcaagc aaaatcaagg
    58501 tttcacagcc agtttacttc gacacaggtt tgggtgacgg tcgtatggtc tttgttctcc
    58561 cacgtgaaaa caagacttac tttggtacaa ctgatacaga ctacacaggt gatttggagc
    58621 atccaaaagt aactcaagaa gatgtagatt atctacttgg cattgtcaac aaccgctttc
    58681 cagaatccaa catcaccatt gatgatatcg aaagcaactg ggcaggtctt cgtccattga
    58741 ttgcagggaa cagtgcctct gactataatg gtggaaataa cggtaccatc agtgatgaaa
    58801 gctttgacaa cttgattgcg actgttgaat cttatctctc caaagaaaaa acacgtgaag
    58861 atgttgagtc tgctgtcagc aagcttgaaa gtagcacatc tgagaaatat ttggatccat
    58921 ctgcagtttc tcgtgggtct agcttggacc gtgatgacaa tggtcttttg actcttgctg
    58981 gcggtaaaat cacagactac cgtaagatgg ctgaaggagc tatggagcgc gtggttgaca
    59041 tcctcaaagc agaatttgac cgtagcttta aattgatcaa ttctaaaact taccctgttt
    59101 caggtggaga attgaaccca gcaaatgtgg attcagaaat cgaagccttt gcgcaacttg
    59161 gagtatcacg tggtttggat agcaaggaag ctcactatct ggcaaatctt tacggttcaa
    59221 atgcaccgaa agtctttgca cttgctcaca gcttggaaca agcgccaggt cttagcttgg
    59281 cagacacctt gtcccttcac tatgcaatgc gcaatgagtt gactcttagc ccagttgact
    59341 tccttcttcg tcgtaccaat cacatgctct ttatgcgtga tagcttggat agtatcgttg
    59401 agccaatttt ggatgaaatg ggacgattct atgactggac agaagaagaa aaagcaactt
    59461 accgtgctga tgtaaaagca gctcttgctc aaaacgactt agcagaatta aaaaattaag
    59521 aaaaaataaa agaggtggag ggtagcaatc cttgtcgccc gccccttttt taatggagac
    59581 agaaaaatga tgagtgaatt atttggagaa tttttaggga ctttaatcct gattcttcta
    59641 ggaaatggtg ttgttgcagg tgtggttctt ccaaaaacca agagcaacag tgcaggttgg
    59701 attgtgatta ctatgggttg ggggattgca gttgcggttg cagtatttgt atctggaaaa
    59761 ctcagcccag ctcatttaaa tccagctgtg acagttggtg tagctttaaa aggtgacttg
    59821 ccttgggctt ccgttttgcc ttatatctta gcccagttcg caggggctat gctgggtcag
    59881 attttggttt ggttgcaatt caaacctcac tatgaggcag aagaaaatgc aggcaatatc
    59941 ctggcaacct tcagtactgg accagccatc aaggatactg tatcaaactt gattagcgaa
    60001 atccttggaa cctttgtttt ggtgttgaca atctttgctt tgggtcttta cgattttcag
    60061 gcaggtatcg gaacctttgc agtgggaact ttgattgtcg gtatcggtct atcactaggt
    60121 gggacaacag gttatgcctt gaacccagct cgtgaccttg gacctcgtat catgcacagc
    60181 atcttgccaa ttccaaacaa gggagacgga gactggtctt acgcttggat tcctgttgta
    60241 ggccctgtta tcggagcagc cttggcagtg cttgtattct cacttttcta gtttatactc
    60301 ttcgaaaatc aaattcaaac cacgtcagcg tcgccttacc gtactcaagt acagcttgcg
    60361 gctagcttcc tagtttgctc tttgattttc attgagtatt agaaaacaat tatgttgata
    60421 gagcttgggc aagagcccaa tttcagcaaa aaatgaagta aatcttctca taataaaacg
    60481 catcatatca agcacgaaaa ttccacgagg tcaactacag tcagaaagct gaacaacaag
    60541 ccaaaacgcc caaaaaaggc ggcaaaaagc aagcacctgc aagcaacgtg ccgaaatggt
    60601 caaatcctga ttatgtcaac gaattagacc caaaaatcgt tgatatgcta gtagaatttc
    60661 acaagtcaca aggcactttg gaaactcccg aggcgcaagc agaaatcgcc caaaaacgtg
    60721 aagaaatcga gcaaaggaga gctgagcttg aggataaaaa acaagagctt ttgaaccgct
    60781 tgaacaaata gagtttcgca agtattatgc ttacaaatta cttgagcaat taactaaaat
    60841 ataaaccctg cctttatatc taggcagggt ttatatttta gaaattcacg taggttgtta
    60901 cggtttttac atacccagta tagtttgagt ttctatagta ttcagtgata aacttccatt
    60961 ttctttgagc aacatggata taagtacttg ttatgtagta tggatatggg ctttgtgaat
    61021 ccaagtaaga ctgataagct tgtataccaa aatatgctcc accaattatt gcaccccatg
    61081 gaccccccaa taaagcacct atcctaccaa tcatataact gattccagca ccagtcatga
    61141 agttagcgaa tgtgttagct tgtttattcc catgtattgt gttgacgtaa ttccaaacat
    61201 taggatcgta tgatctaaaa gatatattta ggtcgatttc attcttttga taagccatat
    61261 aaaatgcccc attgatatag acgccgtcag cacgtcgttc aatagtgtct acacttccat
    61321 ctggattgac aacctcaaga acttcatcgc ttaaaatatt tacttgcgta tctccgaacc
    61381 gcactgatga gccattctca aactgagcct caccagatac aactttagag tttgccgata
    61441 agctatcatc agcaaaaaca acaagcgacg gggaaatgct agacatacag aaaacagaca
    61501 taactagcaa acacatgcat ttaaacatct tagacataac ggaaactcct ttgtattttt
    61561 gatttttttc aacttttatt atacaataaa accaaataaa aagaaagcgg taacaatatg
    61621 cttaatgcga aaatttttta tatattttta tgtttgatcg ttatcgaaac tacaggcttg
    61681 ttgttgttga aaagaggtct cgaaatgggt tatttagaca cagaagctat tatcctcgca
    61741 gttttttcat ttgcttttta caacctatgt tcattcgctt gggtctgctc tacaataaaa
    61801 aacaataaaa aataaataga cg
//
LOCUS       DAVKLW010000003        58510 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKLW010000003 DAVKLW010000000
VERSION     DAVKLW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.003"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            444..1919
                     /locus_tag="VW324_000146"
     CDS             444..1919
                     /locus_tag="VW324_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9725851.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1885..2600)
                     /locus_tag="VW324_000147"
                     /pseudo
     CDS             complement(1885..2600)
                     /locus_tag="VW324_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2856..3716
                     /gene="speE"
                     /locus_tag="VW324_000148"
     CDS             2856..3716
                     /gene="speE"
                     /locus_tag="VW324_000148"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9725852.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            3713..4972
                     /locus_tag="VW324_000149"
     CDS             3713..4972
                     /locus_tag="VW324_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9725853.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4972..6099
                     /gene="nspC"
                     /locus_tag="VW324_000150"
     CDS             4972..6099
                     /gene="nspC"
                     /locus_tag="VW324_000150"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9725854.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6096..7181
                     /gene="aguA"
                     /locus_tag="VW324_000151"
     CDS             6096..7181
                     /gene="aguA"
                     /locus_tag="VW324_000151"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9725855.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7191..8066
                     /gene="aguB"
                     /locus_tag="VW324_000152"
     CDS             7191..8066
                     /gene="aguB"
                     /locus_tag="VW324_000152"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9725856.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8247..9056)
                     /locus_tag="VW324_000153"
     CDS             complement(8247..9056)
                     /locus_tag="VW324_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9725857.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            9328..9549
                     /locus_tag="VW324_000154"
     CDS             9328..9549
                     /locus_tag="VW324_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9725858.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            9603..10274
                     /locus_tag="VW324_000155"
     CDS             9603..10274
                     /locus_tag="VW324_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9725859.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(10326..10523)
                     /locus_tag="VW324_000156"
     CDS             complement(10326..10523)
                     /locus_tag="VW324_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9725860.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            10516..11424
                     /locus_tag="VW324_000157"
     CDS             10516..11424
                     /locus_tag="VW324_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9725861.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11421..11882
                     /locus_tag="VW324_000158"
     CDS             11421..11882
                     /locus_tag="VW324_000158"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9725862.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            11872..12759
                     /locus_tag="VW324_000159"
     CDS             11872..12759
                     /locus_tag="VW324_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9725863.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            12762..14645
                     /gene="cbpE"
                     /locus_tag="VW324_000160"
                     /gene_synonym="pce"
     CDS             12762..14645
                     /gene="cbpE"
                     /locus_tag="VW324_000160"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9725864.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            14725..15855
                     /locus_tag="VW324_000161"
     CDS             14725..15855
                     /locus_tag="VW324_000161"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9725865.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            15865..17127
                     /locus_tag="VW324_000162"
     CDS             15865..17127
                     /locus_tag="VW324_000162"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9725866.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            17131..17928
                     /gene="proC"
                     /locus_tag="VW324_000163"
     CDS             17131..17928
                     /gene="proC"
                     /locus_tag="VW324_000163"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9725867.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            18148..18786
                     /locus_tag="VW324_000164"
     CDS             18148..18786
                     /locus_tag="VW324_000164"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9725868.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            18783..19673
                     /locus_tag="VW324_000165"
     CDS             18783..19673
                     /locus_tag="VW324_000165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9725869.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLQT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            19713..20030
                     /gene="yabA"
                     /locus_tag="VW324_000166"
     CDS             19713..20030
                     /gene="yabA"
                     /locus_tag="VW324_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9725870.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            20033..20902
                     /gene="rsmI"
                     /locus_tag="VW324_000167"
     CDS             20033..20902
                     /gene="rsmI"
                     /locus_tag="VW324_000167"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9725871.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            21129..21647
                     /locus_tag="VW324_000168"
     CDS             21129..21647
                     /locus_tag="VW324_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9725872.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            21756..21893
                     /locus_tag="VW324_000169"
     CDS             21756..21893
                     /locus_tag="VW324_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9725873.1"
                     /translation="MDFEYFYNREAKRFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     K"
     gene            21900..22334
                     /locus_tag="VW324_000170"
     CDS             21900..22334
                     /locus_tag="VW324_000170"
                     /inference="COORDINATES: protein motif:HMM:NF018653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9725874.1"
                     /translation="MSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDIK
                     KGIGLIERVRLGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQ
                     EVKNLDSNYIENNKSKYSKREYSFGENGLGTFQNVFLAAEDI"
     gene            complement(22392..23199)
                     /locus_tag="VW324_000171"
     CDS             complement(join(22392..22826,22828..23199))
                     /locus_tag="VW324_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9725875.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIIDATEVKINRPKKELANYSGKKKCYAMKAQAIVTSQGRIVSLDITVN
                     YCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKFKPLTVEDKTYNH
                     ALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            23194..23448
                     /locus_tag="VW324_000172"
     CDS             23194..23448
                     /locus_tag="VW324_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9725876.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            23792..25126
                     /gene="trmFO"
                     /locus_tag="VW324_000173"
     CDS             23792..25126
                     /gene="trmFO"
                     /locus_tag="VW324_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9725877.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            25210..25947
                     /locus_tag="VW324_000174"
     CDS             25210..25947
                     /locus_tag="VW324_000174"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9725878.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            25956..26513
                     /gene="frr"
                     /locus_tag="VW324_000175"
     CDS             25956..26513
                     /gene="frr"
                     /locus_tag="VW324_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9725879.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            26573..27427
                     /gene="cvfB"
                     /locus_tag="VW324_000176"
     CDS             26573..27427
                     /gene="cvfB"
                     /locus_tag="VW324_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9725880.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            27436..27651
                     /locus_tag="VW324_000177"
     CDS             27436..27651
                     /locus_tag="VW324_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9725881.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            27737..28705
                     /locus_tag="VW324_000178"
     CDS             27737..28705
                     /locus_tag="VW324_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9725882.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(28683..28862)
                     /locus_tag="VW324_000179"
     CDS             complement(28683..28862)
                     /locus_tag="VW324_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725883.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            28898..29398
                     /locus_tag="VW324_000180"
     CDS             28898..29398
                     /locus_tag="VW324_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9725884.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            29401..29661
                     /locus_tag="VW324_000181"
                     /pseudo
     CDS             29401..29661
                     /locus_tag="VW324_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(30028..30354)
                     /locus_tag="VW324_000182"
     CDS             complement(30028..30354)
                     /locus_tag="VW324_000182"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725885.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(30300..30713)
                     /locus_tag="VW324_000183"
     CDS             complement(30300..30713)
                     /locus_tag="VW324_000183"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9725886.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(30714..31139)
                     /locus_tag="VW324_000184"
     CDS             complement(30714..31139)
                     /locus_tag="VW324_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9725887.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            31316..31885
                     /locus_tag="VW324_000185"
     CDS             31316..31885
                     /locus_tag="VW324_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9725888.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            31953..32603
                     /locus_tag="VW324_000186"
     CDS             31953..32603
                     /locus_tag="VW324_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU9725889.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            32587..34827
                     /locus_tag="VW324_000187"
     CDS             32587..34827
                     /locus_tag="VW324_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9725890.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            34969..35193
                     /locus_tag="VW324_000188"
     CDS             34969..35193
                     /locus_tag="VW324_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725891.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYYSYSEYPVGSGWNYARYEVINYYTGGY"
     gene            35225..35812
                     /locus_tag="VW324_000189"
     CDS             35225..35812
                     /locus_tag="VW324_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9725892.1"
                     /translation="MLRLTHVTLKTRQVILQDADFTFKKGRVYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            35816..36997
                     /locus_tag="VW324_000190"
     CDS             35816..36997
                     /locus_tag="VW324_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725893.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVVVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    37157..37288
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            37304..37834
                     /gene="infC"
                     /locus_tag="VW324_000191"
     CDS             37304..37834
                     /gene="infC"
                     /locus_tag="VW324_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9725894.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            37867..38067
                     /gene="rpmI"
                     /locus_tag="VW324_000192"
     CDS             37867..38067
                     /gene="rpmI"
                     /locus_tag="VW324_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9725895.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            38119..38478
                     /gene="rplT"
                     /locus_tag="VW324_000193"
     CDS             38119..38478
                     /gene="rplT"
                     /locus_tag="VW324_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9725896.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            38536..38916
                     /locus_tag="VW324_000194"
     CDS             38536..38916
                     /locus_tag="VW324_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9725897.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            39166..39966
                     /locus_tag="VW324_000195"
     CDS             39166..39966
                     /locus_tag="VW324_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9725898.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            39977..40915
                     /locus_tag="VW324_000196"
     CDS             39977..40915
                     /locus_tag="VW324_000196"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9725899.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            40957..42990
                     /locus_tag="VW324_000197"
     CDS             40957..42990
                     /locus_tag="VW324_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9725900.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(43051..44706)
                     /gene="pavA"
                     /locus_tag="VW324_000198"
     CDS             complement(43051..44706)
                     /gene="pavA"
                     /locus_tag="VW324_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9725901.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            44811..45308
                     /gene="ybeY"
                     /locus_tag="VW324_000199"
     CDS             44811..45308
                     /gene="ybeY"
                     /locus_tag="VW324_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9725902.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            45289..45684
                     /locus_tag="VW324_000200"
     CDS             45289..45684
                     /locus_tag="VW324_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9725903.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            45701..46600
                     /gene="era"
                     /locus_tag="VW324_000201"
     CDS             45701..46600
                     /gene="era"
                     /locus_tag="VW324_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9725904.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            46649..47473
                     /gene="mutM"
                     /locus_tag="VW324_000202"
     CDS             46649..47473
                     /gene="mutM"
                     /locus_tag="VW324_000202"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9725905.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            47473..48078
                     /locus_tag="VW324_000203"
     CDS             47473..48078
                     /locus_tag="VW324_000203"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9725906.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFEQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            48065..49264
                     /locus_tag="VW324_000204"
     CDS             48065..49264
                     /locus_tag="VW324_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEU9725907.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            49264..49413
                     /gene="rpmG"
                     /locus_tag="VW324_000205"
     CDS             49264..49413
                     /gene="rpmG"
                     /locus_tag="VW324_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9725908.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKEKKVTL
                     HLESK"
     gene            49453..49686
                     /gene="secG"
                     /locus_tag="VW324_000206"
     CDS             49453..49686
                     /gene="secG"
                     /locus_tag="VW324_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9725909.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            49788..52142
                     /gene="rnr"
                     /locus_tag="VW324_000207"
     CDS             49788..52142
                     /gene="rnr"
                     /locus_tag="VW324_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9725910.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIVEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGIGRGSNRRSDKKEDKRKS
                     GRSNDKRKYSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            52105..52572
                     /gene="smpB"
                     /locus_tag="VW324_000208"
     CDS             52105..52572
                     /gene="smpB"
                     /locus_tag="VW324_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9725911.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            52588..53448
                     /gene="tehB"
                     /locus_tag="VW324_000209"
     CDS             52588..53448
                     /gene="tehB"
                     /locus_tag="VW324_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9725912.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            53521..54474
                     /gene="coiA"
                     /locus_tag="VW324_000210"
     CDS             53521..54474
                     /gene="coiA"
                     /locus_tag="VW324_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9725913.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            54493..56295
                     /gene="pepF"
                     /locus_tag="VW324_000211"
     CDS             54493..56295
                     /gene="pepF"
                     /locus_tag="VW324_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9725914.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            56297..57010
                     /locus_tag="VW324_000212"
     CDS             56297..57010
                     /locus_tag="VW324_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9725915.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            57077..58018
                     /gene="prsA"
                     /locus_tag="VW324_000213"
     CDS             57077..58018
                     /gene="prsA"
                     /locus_tag="VW324_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9725916.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(58135..58476)
                     /locus_tag="VW324_000214"
                     /pseudo
     CDS             complement(58135..58476)
                     /locus_tag="VW324_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        17593 a        10704 c        13250 g        16963 t
ORIGIN      
        1 tttttatcaa aaaatacttt acaagttctt aaaaacatga tatagtaata aagcttagaa
       61 aatgagatga tgttttctag caaatataaa cccgagtaaa aaatgcctac ggacaggcag
      121 ggttgaatgc cgaagcgtgg ttgaaaagcc acattattga tagggttaaa agcctacttt
      181 tataagttga tgttaggaca cttgtcctaa ttcataaatt tttagtgtgg tgaaagcaca
      241 cgtcatcttg tgaaacgatc aataaagtac gtaatatttg ctactagaga gttaggaaac
      301 atcgggaaca gacatactca acagaaacca aaataaacac gtcagaagat tgcagagcag
      361 gtgaaaacct gctctttttt catgagtcaa cctttagttc cttagttttc ataaggtcct
      421 aaaaatattg aaaggagtat gttttgaaag agttagatca aaaccaagcc ccaatttatg
      481 aggccttggt gaagttgcgc aagaaaagga ttgttccctt tgatgttcca ggtcacaagc
      541 gtggacgggg aaatccagaa cttgtcgaac tcttaggaga aaaatgtgta ggcattgatg
      601 tcaattcgat gaaacccttg gataatttag gccatcctat ttcgattatt cgtgatgcag
      661 aggagctggc tgcagatgct tttggagcta gccatgcctt tctaatgatt ggtggaacaa
      721 cttcatcggt gcagactatg attctggcaa cctgcaaggc aggagataag attattctgc
      781 cacgaaatgt ccataaatct gctatcaatg cgttggttct atgtggtgcc attcccatct
      841 atatcgagat gagtgtagat cctaagattg gtatcgcttt aggtcttgaa aatgaccgag
      901 tagcacaggc cataaaggac catccagatg ctaaggctat cctaatcaac aatcctactt
      961 actacggcat ctgttcagac ctaaaggggt tgacagaaat ggctcatgaa gctggcatga
     1021 tggttttagt agatgaagcc cacggagcgc atttgcattt cactgataaa cttccaattt
     1081 ctgctatgga tgcaggggct gatatggcag cagtttccat gcataagtct ggtgggagtt
     1141 tgacccaaag ctccatttta cttatcgggg agcagatgaa ttctgaatac gttcgtcaga
     1201 taattaacct gacccagtct acatctgcct cttacttgtt gatggctagt ttggatattt
     1261 cacgtcgcaa cttggccctt cgtggtaaag agtcgtttga ggaagtcatt gagctatctg
     1321 agtatgcccg ccgtgaaatc aatgctatcg gtggctacta tgcctactca aaagagttaa
     1381 tagacggtgt ttcggtttgc gattttgacg taactaagtt gtcagtttac actcagggta
     1441 ttggcttaac aggtatcgag gtttatgacc tcttgcgaga cgaatacgac attcagatcg
     1501 agtttggtga tatcggcaat atcttggcct atatttccat cggcgaccgc atccaagaca
     1561 tcgagcgctt ggttggtgct ctggctgata ttaagagact ctattcaaga gatggaaaag
     1621 atttgatagc aggagaatat attcagcccg agttagtgct gtctccgcaa gaagccttct
     1681 attcagaaag aaaaagttta actttggatg attctgttgg acaggtctgt ggagaatttg
     1741 ttatgtgtta ccctccaggt attcctatct tggctcctgg tgaacgcatt acacgagaaa
     1801 ttgtcgacta tatccaattc gccaaggaac gtggttgctc cctccaaggg acggaagatc
     1861 cagaggtcaa tcatatcaac gttattaaga gaaagacaaa ctataagaaa agtcaatagt
     1921 ttatctaaac tatttcttat ttcaatttga tgatttggcg atgattttag agcacggcaa
     1981 aaagcccttg aattagaagc ggtcaatcgc ttaatttcta tcagcttatc aaatcctgcc
     2041 tcaatccttt tctgaggatt agggtagcgt gtcaagagtt ggtaggtata ttctgaatgc
     2101 tttccaacga ttttatccaa ctcaggaaag atgatatcaa ggcaacgagt gtattgtact
     2161 ttccaatcag actgtttttt cttgagacga tgaatatgtc tagtcagtat ttttagttct
     2221 acttgccgat tattgtgttg aaattgttca cgattggggt cagaaagaag tttaagagcg
     2281 atgccatgag cgtctttctt atccgtttta gtcttgcgaa gtgataatga tttggcaaat
     2341 ttcttgatga gcaaaggatt gtaggtgtaa actttatatc cttgttcatg caggaagttc
     2401 agtagattaa aggcataatg tccagtatct tcaagagcga tgagacagtc ttggttgagc
     2461 tgtcgaagag acagatctaa gagttcaaaa ccagctttat tatttgaaaa agtgagtggt
     2521 ttaagaacag tttttccttg aacattcaag gctgtaacat cgtgtttatt tttagcgaca
     2581 tcaatgccca cataaggcat gggagtacct ccagatgtag tatttcaagt ctacttggtt
     2641 atccacgaac tttttgccct gttaccttag acgagataaa acgtctatgc gttatcaaac
     2701 tcattaccaa ttgaaacaaa aagctgtggt tagagccttt aggaaatcgt caagcgattg
     2761 gaggaaatga actaatccac agtggcttat tccaagtata ccacttgggc tttggcagta
     2821 gctaactgcg ctaaatataa tataaggaga aatagatgga tttatggttt tctgaagttc
     2881 atactccaga tgtcaaattg tctctgagaa cagccaagca actttacgct ggaaaaagtg
     2941 aatggcagga tatcgaagtc ttggatacgc cagcttttgg gaaaatactg attttaaatg
     3001 gccatgtctt gttctcagat gcggatgatt tcgtctacaa tgaaatgacc gtccacgttc
     3061 ccatggctgt ccacccaaat ccaaagaaag tattggttat tgggggtggt gacggcggtg
     3121 ttgcccaagt attaacactc tatcctgaac tggaacaaat cgatatcgtg gaaccggatg
     3181 agatgttggt cgaggtctgt cgtgagtatt tcccagactt tgctgcaggc ctagatgatc
     3241 ctcgtgttac tatttactac caaaatgggc tacgcttttt gcgaaactgc gaagatgact
     3301 atgatattat catcaacgat gcgacagatc catttggcca tacggaagga ctctttacca
     3361 aggaattcta cggcaatagt tatcgagctc tgaaggaaga cggcatcatg atttaccagc
     3421 atgggagtcc cttctttgac gaggatgagt cggcctgccg aagcatgcac cgcaaggtca
     3481 atcaagcctt tccaatcagt cgggtttatc aggcccatat tccaactagc ccagctggct
     3541 attggttgtt tggatttgca tcgaaaaaat accaccctgt caaagatttt gacaaggaag
     3601 gctggaaaaa acgccagctt ttcacagaat actacactgc aaacttacac gtgggagcct
     3661 ttatgttgcc caagtatgtt gaggacattt tagaagaaga ggaaggaaaa aaatgagtcg
     3721 tttactagtt attggttgtg ggggcgttgc ccaagttgct atttcaaaga tttgtcaaga
     3781 tagcgaaaca tttacagaga ttatgattgc tagccgtacc aagtcaaaat gcgatgactt
     3841 gaaagcgaag ctagaaggca aaacaagtac taaaattgaa actgcagcac ttgatgctga
     3901 caaggttgaa gaagtgattg ccctgattga aagctacaaa ccagaagctg ttttgaatgt
     3961 agctctgcct tatcaagatt taaccattat ggatgcttgt ttggcaacag gtgttcacta
     4021 tatcgataca gccaactacg aagcagaaga cacagaagac cctgagtggc gtgctatcta
     4081 cgaaaaacgt tgtaaggaac ttggttttac agcctacttt gactactcat ggcagtgggc
     4141 ttatcaagag aaattcaaag aagcaggctt gactgctctt cttggttctg gttttgaccc
     4201 aggtgtaact agtgtctttt cagcttatgc cctcaaacac tattttgatg aaatccatta
     4261 tatcgacatt ttagactgta atggcggtga ccacggttat ccatttgcaa ccaactttaa
     4321 tccagaaatt aatctccgtg aggtttctgc gccaggttct tactgggaag atgggaaatg
     4381 ggtcgaagtc gaagctatgt ctatcaagcg tgagtatgat ttccctcaag ttggacaaaa
     4441 agatatgtat ctccttcacc atgaagaaat cgaatcattg gccaagaaca ttccaggtgt
     4501 caaacgcatt cgtttcttta tgacttttgg tcaatcttac ttgacgcaca tgaaatgtct
     4561 tgaaaatgtt ggactccttc gtacggatac cattaacttt aacggccaag aaattgttcc
     4621 aattcaattt ttgaaagcct tgcttccaga tcctgctagc cttggcccac gtactgtagg
     4681 taaaaccaat attggatgta tctttacagg tgtcaaagat ggcgttgaaa agactatcta
     4741 catctacaat gtttgcgacc atcaagagtg ctatgcagaa gtcgggtcac aagcaatttc
     4801 atacacgaca ggcgttccag ccatgattgg gacaaaatta gtcatgaacg gaacttggaa
     4861 acaagctgga gtgtataacc ttgaggagtt agatccagat ccattcatgg aagctttgaa
     4921 tgagtatggt ttgccatggg ttgtggttga aaatccacaa atggtggact aatgaagtta
     4981 gaacaagtac caacaccagc ctatgttatt gacttggcca agttagaagc taattgccgc
     5041 attctacaat atgtacaaga agaggccggt tgcaaggtct tgcttgccca gaaggcatat
     5101 tccctctaca aaacttatcc cttgattagc cagtatctat caggtacgac agctagtgga
     5161 ctctatgagg ccaaattggc aagggaagaa tttcctggtg aagtccatgt ttttgcacca
     5221 gctttcaagg atgaggacat ggaggaattg cttgggataa cagaccatat cgtctttaac
     5281 tcagagagac agttgcgtaa gcatggggct cgttgtcgag aggctggtgt cagtgttggt
     5341 ttgcgcctca accctcagtg ttcaactcaa ggagatcacg cgctctatga cccttgtgca
     5401 cctggttctc gctttggagt tactatagac aagattccga gtgatttgct agatttggtt
     5461 gacggacttc attttcatac cctttgcgag cagggagcag atgatttaca aacaactttg
     5521 aaagcagtag aagaacagtt tggtccctac ttacatgagg taaaatggct caatatgggt
     5581 ggtggtcatc atattacaag agaaggttac gatgtggatt tgctgatttc agaaatcaag
     5641 cgtatccgaa aaacttacaa tcttgaaatc tatatcgagc ctggtgaagc cattgcgctt
     5701 aatgcgggtt atttagcaac tgaggtatta gatattgtag aaaacggtat ggaaatcttg
     5761 gttttagacg cctctgcgac ctgccatatg cctgatgtac ttgagatgcc ctatcgtcca
     5821 cctttgagaa atggctttga gtcacaggaa aaagcccata cctacagact ttcttctaat
     5881 acctgtctga ctggcgatgt gattggtgat tatagttttg aaaatccagt ccaaatcgga
     5941 gacagacttt attttcaaga catggccatt tattcttttg tcaaaaataa tacctttaat
     6001 ggtattggat tgccaagtct ctatctcatg gacgaacagg gagactgtag cttactcaaa
     6061 gcttttggct atcaagactt taaagggaga ttatcatgat ggacagtcca aaaaaattag
     6121 gctatcacat gccagcagag tacgaacccc atcatggtac cctcatgata tggccgactc
     6181 gaccaggatc atggcctttt caaggaaagg ctgctaaaag agcatttact cagatcatcg
     6241 agaccatagc agaaggggaa agagtctatc ttttggtgga gcaggcctat ctatctgaag
     6301 cccaatccta tcttggagac aaggttgttt atttagacat tcccaccaat gatgcctggg
     6361 cgcgtgatac tggcccaacc attctcgtca atgataaagg taagaaatta gccgtggatt
     6421 gggcctttaa tgcttgggga ggcacctatg atggtcttta tcaagattat gaagaggatg
     6481 accaagtagc cagtcgtttt gctgaggcct tggaaaggcc tgtctatgat gctaaacctt
     6541 ttgtactgga aggaggcgca atccatagcg atggtcaagg aactattctc gtaactgaaa
     6601 gttgcttgct tagtcctggt cgcaatccta acctgactaa agaggagatt gaaaacacat
     6661 tattagaaag tcttggtgct gaaaaagtta tttggcttcc ttatggtatt tatcaggatg
     6721 aaaccaatga acacgtcgat aatgttgttg cctttgttgg tcctgctgag gttgttttgg
     6781 cttggacaga tgacgaaaat gatccccagt atgccatgtc aaaagcagat ctcgaactct
     6841 tagaacagga aacagatgca aaaggttgtc acttcaccat tcataaattg cctatccctg
     6901 cagttcgaca agttgtgaca gaagaagatt tgccaggcta catctatgaa gaaggagaag
     6961 aagagcgata cgcaggtgaa cgactagcag cttcctacgt aaacttttat atcgccaaca
     7021 aggctgtctt ggttccacag tttgaggatg taaacgacca agtggcctta gatatcctca
     7081 gcaagtgttt cccagaccgt aaagttgtcg gaataccagc cagagatatt ctcttaggtg
     7141 gtggcaatat ccactgtatc acccaacaaa ttccagaata ggagaaaaag atgagaaatg
     7201 taagagttgc aaccattcag atgcaatgcg ctaaggatgt ggcaacaaat atccaaaccg
     7261 cagagcgttt agtacgtcag gctgctgagc aaggagccca aattattctc ttgcccgagt
     7321 tgtttgaaca tccctatttc tgtcaggaac gtcagtatga ctactaccag tatgcccaat
     7381 ctgtagcgga aaatactgcc attcagcatt ttaaggtgat tgctaaggaa ctacaagttg
     7441 ttttaccaat cagtttctat gaaaaagatg gtaatgtctt gtataactct attgccgtca
     7501 ttgatgcaga tgggggagtg ctgggcgttt atcgaaagac ccatatacca gatgaccatt
     7561 attatcaaga aaaattctat ttcacgcctg gtaacactgg tttcaaggtc tggaatactc
     7621 gctatgctaa gattggtatc ggtatctgtt gggatcaatg gttccctgaa acagcccgct
     7681 gtcttgcatt gaatggtgct gaattgctct tttatcctac agctatcggt tcagagccaa
     7741 ttttggatac agatagttgt ggtcactggc aacgtactat gcaagggcac gcagcagcga
     7801 atattgttcc agtcatcgca gccaatcgtt atggtttaga ggaggttact cctagtgagg
     7861 aaaatggcgg acagagttcc agtcttgact tctacggttc ctcctttatg acggatgaaa
     7921 caggagctat tctagaacga gctgaaagac aagaagaagc tgttctgtta gctacttatg
     7981 acctagacaa gggagcaagt gaacgtctaa actggggctt gtttcgagat agaagaccag
     8041 aaatgtatag acaaattaca gattagtgtg ggagaaatga gagattcatt ctgctagact
     8101 aacttcttat tagcaactat aagatactat ggcatctagt aaatcgattt ttatgattcg
     8161 ctattcttgt ctattgatta gtccgtattt taaaatatta gcaaaaaagc aaatagcagt
     8221 aacttctgtc tatttgcttt tcttttttat agaatatatt tctcaatagc acgcgcaacg
     8281 ccgtcttctt cgttgcttga ggtaacggca tccgcaagag atttgacata atcgctggca
     8341 tttcccattg caatcccaag ccctgcaaac tggagcattt cgatatcgtt attagcatcg
     8401 cccatggcca taatctctga ggaatcaatc ttcaaaatct cagctagtcg tgaaagagca
     8461 gtagcctttg tcgttccaag cggcattgct tcataaatga caggctgcga acgaactcca
     8521 ctgaatcgtt ggcaaagctc ttcagcaaaa cgctgctcaa aatcgtctgt ttgttctttt
     8581 gttcctaaaa acataccttg gaacatccgg aactttccac tagtcgcttc ttcaagagaa
     8641 atttcagtca ggtctgaaaa tactagttta gcatcatttt caataacttg attgggtttg
     8701 ccaccgagaa caaaataatg tgactcgtca aaaagtgtca actgaacatc actcttttca
     8761 gcaaggtcat agaggtattc gatgtcagct ggactcagtt ctttccagtc aactagactc
     8821 caatcactgg tctggtgagt tgaacaaccg ttgttaacaa taatatattc gttctggagg
     8881 tcaagctcca gttttttgta gtaggggagg acaccgaaaa gggggcgacc cgtacagaga
     8941 accagtttga cacctttttc aatggctttg tgaatagcag taatgtgagc ttgtgggatt
     9001 tccttggctt cattgaggag ggtgccgtcc atatccaagg ctagtagttt aatcataggt
     9061 cttccttctt tatcttttgc tattattata gcatattttg gagaagaaat tgatagaaag
     9121 cttgagacta attgatttta tagtttaaga tgttttgatg acaattcatg atttgaagag
     9181 gatatttggc aaagatatgc tatactatgt ttgtcaatgt tgcaactaga caaattaaaa
     9241 aaccaactta atataatagt ttttttgtaa gtaggtatga gtatcagatt actcaactaa
     9301 tctgaagaat aatggaggaa atatatcatg attttaatga caaaaaatat aaatctaaca
     9361 aatgaagaat tagagctgat acaaggtgga gcagattcat atggtaaaaa tcctaatggt
     9421 aggtacgatt gggaaataga accagtatta actctgccgg ttcatggatt ttgttccaga
     9481 ggcacctatg atttaggata tattggagga ggcaatcatc tttgcaaagg aagtgctgcg
     9541 agattttaag taaaatttat tagtaatatg aagaaacaag tggagaaagc agagaattta
     9601 atatgaaaaa acgggctatt caaattttac tagcattgtc cttaattttt tacaaatcaa
     9661 cttggttttg gagagttttc aatcacctcg caaagcccta tctaccagca agtcgtgaat
     9721 tttttcagat tctgcttttg atggagagcg gagttctttt cttagcggtc atctatctac
     9781 tggtttttgc aggaaagaaa atttttcatt tcaagtggca gctgaggtac ttcatctacc
     9841 ttttactggg ctacatcatt tcatatatgt ctgacttcct cttttcgtat ttcatatccc
     9901 tgtcttcaaa tcagatttct ttgaatgaaa cgatagaaat gatggggaga caggagttcc
     9961 cttatttctt gctcatcgtt tgcttcatcg cccctattgc tgaggaattg atttatcgag
    10021 gtgtgcttat gacaaccttt ttcaaaaact caccttggta cggagatgtt ttgctttctg
    10081 ctattatttt cggttatatt catatcaatt ttgctttaac tcctcttgct tttttcattt
    10141 atgctagtgg aggtctgatt ttagctctat tgtatcgcat gactaaaaat ctctactatc
    10201 caatactagt tcatattctc atcaatatca ctgccttctg ggatgtttgg ttactcctat
    10261 tttcaggaag ttagcttact aaaaaaatgt cggaattttc cggcattttc ttttttcaca
    10321 aatagtcaac gtttttcttt ccgatactga agtggtgtgt agccacttat ttttttgaat
    10381 tgattttgaa aataagattg gcgtgagaaa ggcagatagt gaagatagtt aagaagaata
    10441 ggatgttctt ttttcctttt tggaaaactt ctaaaatatg gtataatgaa aagataaaga
    10501 agttgggggt agaagatgaa cattcaacaa ttacgctatg ttgtggctat tgccaatagt
    10561 ggtacttttc gtgaagctgc tgaaaagatg tatgttagtc agccgagtct gtctatttct
    10621 gttcgtgatt tggaaaaaga gttgggcttt aagattttcc gtcggaccag ctcagggact
    10681 ttcttgaccc gtcgtgggat ggaattttat gaaaaagcgc aagaattggt taaaggattt
    10741 gatatttttc aaaatcagta tgccaatcct gaagaagaaa aagatgaatt ttccgttgct
    10801 agccagcact atgacttctt accaccaact attacggcct tttcagagcg ctatcctgac
    10861 tataagaact tccgtatttt tgaatcaact actgttcaaa tattagatga agtggcgcaa
    10921 gggcatagtg agattgggat tatctacctc aacaatcaaa ataaaaaggg gattatgcaa
    10981 cgggttgaaa agttaggtct ggaggtcatc gaattgattc ctttccatac ccatatttat
    11041 ctctgtgagg gtcatccttt agcccagaaa gaggaattag tcatggagga tttagcggat
    11101 ttaccaacgg ttcgtttcac tcaagagaaa gacgagtacc tttattattc agagaacttt
    11161 gtcgatacca gcgctagctc acagatgttt aatgtgacag accgtgccac cttgaatggt
    11221 attttggagc ggacggacgc ctatgcgaca ggttctggat ttttagatag tgacagtgtt
    11281 aatggcatta cagttattcg tctcaaggat aacctagata accgcatggt ctatgttaaa
    11341 cgtgaagaag tggagcttag tcaagctggg actctcttcg tagaagtcat gcaagaatat
    11401 tttgatcaaa agaggaaatc atgaaaaaaa gagcaatagt ggcagtcatt gtactgcttt
    11461 taattgggct ggatcagttg gtcaaatctt atatcgtcca gcagattcca ctgggtgaag
    11521 tgcgctcctg gatccccaat ttcgttagct tgacctacct gcaaaatcga ggtgcagcct
    11581 tttctatctt acaagatcag cagttgttat tcgctgtcat tactctggtt gtcgtgatag
    11641 gtgccatttg gtatttacat aaacacatgg aggactcatt ctggatggtc ttgggtttga
    11701 ctctaataat cgcgggtggt cttggaaact ttattgacag ggtcagtcag ggctttgttg
    11761 tggatatgtt ccaccttgac tttatcaact ttgcaatttt caatgtggca gatagctatc
    11821 tgacggttgg agtgattatt ttattgattg caatgctaaa agaggaaata aatggaaatt
    11881 aaaattgaaa ctggtggtct gcgtttggat aaggctttgt cagatttgtc agaattatca
    11941 cgtagtctcg cgaatgaaca aattaaatca ggccaggtct tggtcaatgg tcaagtcaag
    12001 aaagctaaat acacagtcca agagggtgat gtcgtcactt accatgtgcc agaaccagag
    12061 gtattagagt atgtggctga ggatcttccg ctagaaatag tctaccaaga tgaggatgtg
    12121 gctgtcgtta acaaacctca gggaatggtt gtgcacccga gtgctggtca taccagtgga
    12181 accctagtaa atgccctcat gtatcatatt aaggacttgt cgggtatcaa tggggttctg
    12241 cgtccaggga ttgttcaccg tattgataag gatacgtcag gtcttctcat gattgctaaa
    12301 aacgatgatg cgcatctagt acttgcccaa gaactcaaag ataaaaagtc tctccgcaaa
    12361 tattgggcga ttgttcatgg aaatctgcct aatgatcgtg gtgtaattga agcgccgatt
    12421 ggccggagtg aaaaagaccg taagaaacag gctgtaactg ctaaagggaa gcctgcagtg
    12481 acgcgttttc acgtcttgga acgctttggc gattatagct tagtagagtt gcaactggag
    12541 acagggcgca ctcatcaaat ccgtgtccac atggcttata tcggccattc agtcgctggt
    12601 gatgaggtct atggtcctcg caagactttg aaaggacatg gacaatttct tcatgccaag
    12661 actttaggtt ttactcatcc gagaacaggt aagaccttgg aatttaaagc agatatccca
    12721 gagattttta aggaaacctt ggagagattg agaaagtaag aatgaaaaag aaattaacta
    12781 gtttagcact tgtaggcgct tttttaggtt tgtcatggta tgggaatgtt caggctcaag
    12841 aaagttcagg aaataaaatc cactttatca atgttcaaga aggtggcagt gatgcgatta
    12901 ttcttgaaag caatggacat tttgccatgg tggatacagg agaagattat gatttcccag
    12961 atggaagtga ttctcgctat ccatggagag aaggaattga aacgtcttat aagcatgttc
    13021 taacagaccg tgtctttcgt cgtttgaagg aattgggtgt ccaaaaactt gattttattt
    13081 tggtgaccca tacccacagt gatcatattg gaaatgttga tgaattactg tctacctatc
    13141 cagttgaccg agtctatctt aagaaatata gtgatagtcg tattactaat tctgaacgtc
    13201 tatgggataa tctgtatggc tatgataagg ttttacagac tgctgcagaa aaaggtgttt
    13261 cagttattca aaatatcaca caaggggatg ctcattttca gtttggggac atggatattc
    13321 agctctataa ttatgaaaat gaaactgatt catcgggtga attaaagaaa atttgggatg
    13381 acaattccaa ttccttgatt agcgtggtga aagtcaatgg caagaaaatt taccttgggg
    13441 gcgatttaga taatgttcat ggagcagaag acaagtatgg tcctctcatt ggaaaagttg
    13501 atttgatgaa gtttaatcat caccatgata ccaacaaatc aaataccaag gatttcatta
    13561 aaaatttgag tccgagtttg attgttcaaa cttcggatag tctaccttgg aaaaatggtg
    13621 ttgatagtga gtatgttaat tggctcaaag aacgaggaat tgagagaatc aatgcagcca
    13681 gcaaagacta tgatgcaaca gtttttgata ttcgacaaga cggtttagta aacatttcaa
    13741 cttcctacaa gccgattcca agttttcaag ctggttggca taagagttca tatggaaact
    13801 ggtggtatca agcgcctgat tctacaggag aatatgctgt cggttggaat gaaattaagg
    13861 gtgaatggta ttactttaac caaacgggta tcttattaca gaatcaatgg aaaaaatgga
    13921 acaatcgttg gttctatttg acagactctg gtgttgctgc taaaaattgg aagaaaatcg
    13981 ctggaatctg gtattatttt aacaaagaaa accagatgga aattggttgg attcaagata
    14041 aagagcagtg gtattatttg gatgttgatg gctctatgaa gacaggttgg cttcaatata
    14101 aggggcaatg gtattacttt gctccatcag gggaaatgaa aacgggctgg gtaaaagata
    14161 aagaagcctg gtactatatg gattctactg gtatcatgaa gacaggtgag atagaagttg
    14221 ctggacatca ttactatcta gaagaatcag gagctatgaa gcaaggctgg cttaaaaagg
    14281 caaatgattg gtatttctac aagacagatg gttcacgagc tataggttgg attaaagaca
    14341 aggataaatg gtacttcttg aaagaaaatg gtcaattact tgtgaatggt aagacaccag
    14401 aaggctatac tgtggattca agtggtgcct ggttagtgga tgttccgatc gagaaatctg
    14461 ctacaattaa aactacaagt cattcagaaa taaaagaatc caaagaagta gtgaaaaagg
    14521 atcttgaaaa taaagaaacg agtcaacatg aaagtgttac aaattcttca actagtcaag
    14581 atttgacatc ctcaacttca caaagctctg aaacgagtgt aaacaaatcg gaatcagaac
    14641 agtagtagaa aagaaggttt tagggccttc tttttcctat caactctttt ctatttcctg
    14701 ttattcatgt tataatggat aaatatgaat aatcggagtg agactatgaa atacaaacgg
    14761 attgtcttta aggtgggtac ttcttctctg acaaatgagg atggaagttt atcacgtagt
    14821 aaggtaaagg atattaccca gcagttggct atgctgcacg aggctggtca tgagttgatt
    14881 ttggtgtctt caggtgccat tgcggctggt tttggagcct taggatttaa aaagcgtccg
    14941 actaaaattg ctgataaaca ggcttcagca gcggtagggc agggactttt gttggaagaa
    15001 tatacgacca atcttctctt gcgtcaaatc gtttctgcac aaatcttgct gacccaagat
    15061 gactttgtgg ataagcgtcg ttataaaaat gcccatcagg ctttgtcggt tttgctcaac
    15121 cgtggggcaa ttcctatcat caatgagaat gatagtgtcg ttattgatga gctcaaggtt
    15181 ggggacaatg acactctaag tgctcaagta gcggcgatgg tccaagcaga ccttttagtt
    15241 ttcttgacag atgtggacgg tctctatact ggaaatccta attcagatcc aagagccaaa
    15301 cgcttggaga gaatcgagac catcaatcgt gagattattg atatggctgg tggagctggt
    15361 tcgtcaaacg gaactggggg tatgttaacc aaaatcaagg ctgcaactat cgcgacggaa
    15421 tcaggagttc ctgtttatat ctgctcatcc ttgaaatcag attccatgat tgaggcggca
    15481 gaggagaccg aggatggttc ttactttgtt gctcaagaga aggggcttcg tacccagaaa
    15541 caatggcttg ccttctatgc tcagagtcaa ggttctattt gggttgataa aggggctgcg
    15601 gaagctctct ctcaacatgg aaagagtctt ctcttatctg gtatcgttga agcagaagga
    15661 gtcttttctt acggtgatat cgtgacagta tttgataagg aaagtggaaa atcacttgga
    15721 aaaggacgcg tgcaatttgg agcatctgct ttggaggata tgttgcgttc tcaaaaagcc
    15781 aagggtgtct tgatttaccg tgacgactgg atttccatta ctcctgaaat ccaactactt
    15841 tttacagaat tttagaggta aactatggtg agtagacaag aacaatttga acaggtacag
    15901 gctgttaaaa aatcgattaa cacagctagt gaagaagtga aaaaccaagc cttgctagcc
    15961 atggctgatc acttagtggc tgctactgag gaaattttag cggctaatgc cctcgatatg
    16021 gcagcggcta aggggaaaat ctcagatgtg atgttggatc gtctttattt ggatgcagat
    16081 cgtatagaag cgatggcaag aggaattcgt gaagtggttg ccttaccaga tccaatcggt
    16141 gaagttttag aaacaagtca gcttgaaaat ggtttggtta tcacaaaaaa acgtgtagct
    16201 atgggtgtca tcggtattat ctatgaaagc cgtccaaatg tgacgtctga tgcggctgct
    16261 ttgactctta agagtggaaa tgcggttgtt cttcgtagtg gtaaggatgc ctatcaaaca
    16321 acccatgcca ttgtcacagc cttgaagaag ggcttggaga cgactactat tcatccaaat
    16381 gtgattcaac tggtggagga tactagccgt gaaagtagtt atgctatgat gaaggccaag
    16441 ggctatctag accttctcat tcctcgtgga ggagctggct tgatcaatgc agtggttgag
    16501 aatgcgattg tacctgttat cgagacaggg actgggattg tccatgtcta tgtggataag
    16561 gatgcagacg aagacaaggc gctgtctatc atcaacaatg ctaaaaccag tcgtccttct
    16621 gtttgtaatg ccatggaggt tctgctggtt catgaaaaca aggcagcaag catccttcct
    16681 cgcttggatc aaatgttggt tgcagatcga aaagaagctg ggttggaacc aattcaattc
    16741 cgcctagata gcaaagcaag ccagtttgtt tcaggtcaag ctgctcaagc acaagacttt
    16801 gataccgagt ttttagacta tattctagct gttaaggttg tgagcagttt agaagaagcg
    16861 gttgcgcata ttgaatccca cagtacccat cattcggatg ctattgtgac ggaaaatgct
    16921 gaagctgcag catactttac agatcaagtg gactctgcag cggtgtatgt taatgcctca
    16981 actcgtttca cagatggagg acaatttggt cttggttgtg aaatggggat ttctactcag
    17041 aaattgcacg cgcgtggtcc aatgggcttg aaagagttga ccagctacaa gtatgtggtt
    17101 gctggtgatg ggcagataag ggagtaagag atgaagattg gatttatcgg tttggggaat
    17161 atgggtgcta gcttggcaaa atctgtcttg cagactagga cgtcagatga gattctcctt
    17221 gccaatcgta gtcaagctaa ggtagatgct ttcattgcag actttggtgg tcaggcttcc
    17281 agcaatgaag aaatgtttgc agaagcagat gtgatttttc taggagttaa gcctgctcag
    17341 ttttctgaac tgctttctca atatcagacc atccttgaaa aaagagaaag tcttcttttg
    17401 atttcgatgg cagctggatt gaccttagaa aaactagcaa gtcttatccc aagccaacac
    17461 cgaattattc gtatgatgcc taatacccct gcttctatcg ggcaaggagt gattagttat
    17521 gccttgtctc ctaattgcag ggctgaggac agtgagatct tttgtcagct tttagccaag
    17581 gctggtctct tggttgaatt aggagaaagc ttaatcaatg cagcgacagg tcttgcaggt
    17641 tgtggaccag cctttgtcta tctctttatt gaggccttgg cagatgcagg tgttcagacg
    17701 ggattaccac gagaaacagc cttgaaaatg gcagcccaaa ctgtggtagg agctgggcaa
    17761 ttggtcctag aaagccagca acatcctgga gttttgaaag atcaagtttg tagtccaggc
    17821 ggttcgacta ttgctggcgt agcaagccta gaagcgcatg cttttcgagg cacagtcatg
    17881 gaggcagttc atcaagccta taaacgaaca caagaactag gtaaataaga ggtagtttta
    17941 tctgcctctt ttatggtggt tgatactctt cgaaaatcaa attcaaactg cgtcagcttc
    18001 accttgccgt actcaagtac agcctgcggc tagcttccta gtttgctctt tgattttcat
    18061 tgagtatgaa atgagaagac aaaaagattg tcacaaaccc ctattttttt gatagaatag
    18121 aagtagtaaa aaagaaatga gttagacatg tcaaaaggat ttttagtctc tcttgaggga
    18181 ccagagggag caggcaagac cagtgtttta gaggctctgc taccaatttt aaaggaaaaa
    18241 ggagtagagg tgttgacgac ccgtgaacct ggcggagtct tgattgggga gaagattcgg
    18301 gaagtgattt tggatccaag tcatactcag atggatgcta aaacagagct acttctctat
    18361 attgccagtc gcagacagca tttggtggaa aaagttcttc cagcccttga agctggcaag
    18421 ttggtcatca tggatcgttt tatcgatagt tctgttgcct atcagggatt tggtcgtggc
    18481 ttagatattg aagccattga ctggctcaat cagtttgcga cagatggcct caaacccgat
    18541 ttgacactct attttgacat cgaggtggaa gaagggctgg ctcgtattgc tgcgaatagt
    18601 gaccgcgagg ttaatcgttt ggatttggaa gggttggact tgcataaaaa agttcgtcaa
    18661 ggctaccttt ctcttctgga taaagaggga aatcgcattg tcaagattga tgctagtctc
    18721 cctttggagc aagttgtgga aactaccaag gctgtcttgt ttgacggaat gggcttggcc
    18781 aaatgaaaca agatcaacta aaggcttggc aaccagctca gtttgaccgt tttgtccgta
    18841 tcttagaaca agaccagctc aatcacgcct atctcttttc aggtttcttt ggaagcttgg
    18901 aaatggcgca atttttagct aagagcctct tttgtacgga taaagttggc gtcttaccat
    18961 gtgagaaatg ccgaagttgc aagctgattg aacaggaaga gtttccagat gtcaccttga
    19021 ttaagccagt caatcaggtc atcaagacag aacgcattcg ggaattggtg ggacagtttt
    19081 ctcaagcagg gattgaaagc cagcaacagg tctttattat cgagcaagcg gataaaatgc
    19141 atcccaacgc agccaattct ctgctcaagg tcatcgaaga accccagagt gaagtttata
    19201 ttttcttctt gactagcgat gaggaaaaga tgttaccgac aatccgaagt cggactcaga
    19261 tcttccactt taaaaagcaa gaagaaaaac ttatcttact cttagaacaa atgggacttg
    19321 ttaagaaaaa agcgactctt ttagctaagt ttagtcaatc gcgagctgaa gcagaaaagt
    19381 tggctaatca ggcaagtttt tggaccttgg tcgatgaaag tgaacgcctg cagacttggt
    19441 tagtagctaa gaaaaaagaa agttatctac aggttgccaa attagccaac ttggcagatg
    19501 ataaggaaaa acaggatcag gttttacgga ttcttgaagt tctctgtggg caggacctct
    19561 tgcaggtaag agtaagagtg attctacaag atttactaga agctagaaaa atgtggcaag
    19621 ctaatgtcag ctttcaaaat gccatggaat atctggtctt gaaagaaata taaactcaaa
    19681 aatgaatgat aaagaaagga aagggctgtt ttatggacaa aaaagaatta tttgacgcgc
    19741 tggatgattt ttcccaacaa ttattggtaa ccttagccga tgtggaagcc atcaagaaaa
    19801 atctcaagag cttggtagaa gaaaatacag ctcttcgctt ggaaaatagt aagttgcgag
    19861 aacgcttggg tgaggtggaa gcagacgctc ccgtcaaggc caagcatgtt cgcgaaagtg
    19921 tccgtcgtat ttaccgtgat ggatttcacg tatgtaatga tttttatgga caacgtcgag
    19981 agcaggacga agaatgtatg ttctgtgatg agttgttata cagggagtaa gcatgcaaat
    20041 tcaaaaaagt tttaaggggc agtctcccta tggcaagctg tatctagtgg caacgccgat
    20101 tggcaatcta gatgatatga cttttcgtgc tatccagacc ttgaaagaag tggactggat
    20161 tgctgctgag gatacgcgca atacagggct tttgctcaag cattttgaca tttccaccaa
    20221 gcagatcagt tttcatgagc acaatgccaa ggaaaaaatt cctgatttga ttggtttctt
    20281 gaaagcaggg caaagtattg ctcaggtctc tgatgccggt ttgcctagca tttcagaccc
    20341 tggtcatgat ttggttaagg cagctattga ggaagaaatt gcagttgtta cagttccagg
    20401 tgcctctgca ggaatttccg ccttgattgc cagtggttta gcgccgcagc cacatatctt
    20461 ttacggcttt ttaccgagaa aatcaggcca gcaaaagcaa tttttcgact cgaaaaaaga
    20521 ctatcctgaa actcagattt tttatgaatc gccccatcgt gtggcagata cattggaaaa
    20581 tatgctagaa gtctacggtg accgctccgt tgtcttggtc agggaattga ccaaaatcta
    20641 tgaagaatac caaagaggta caatttctga attgctggaa agcattgctg aaacgtctct
    20701 caagggtgaa tgtcttctga ttgttgaagg tgccagtcag gatgtggaag aaaaaggcga
    20761 ggaagacttg ttcttagaaa tccaagctcg tattcagcaa ggtatgaaga aaaatcaagc
    20821 tattaaggaa gtagctaaga tttaccagtg gaataagagt caactctacg ctgcctacca
    20881 cgactgggaa gaaaaacaat aaagggagac aggatgtaat aattctgtct gtttctgttt
    20941 aacttaatta gtgatgataa tataaagatg tatcacttgg tatagaagct ttggtattaa
    21001 gttttttatt aagcccatac ggaataccga tggttggagc agcagttata gcgttcttag
    21061 aaggtataaa tagaaaaata aggtcatttt aaatcaaagg attgataaat cagaaagaag
    21121 gtgatttttt gcgaacatac gaaaataaag aagaactaaa agctgagata gagaaaacat
    21181 ttgagaaata tattttagaa tttgataata ttccagaaaa tttaaaagat aagagagctg
    21241 atgaagttga cagaactcca gcagaaaacc ttgcttatca ggttggttgg accaacttgg
    21301 ttcttaaatg ggaagaagat gaaagaaagg ggcttcaagt aaaaacacca tcggataaat
    21361 ttaaatggaa tcaacttggt gaattatatc agtggttcac agatacctac gctcatttat
    21421 ctctgcaaga gttgaaagca aaattaaatg aaaatattca ttctatctct gcaatgattg
    21481 attcgttgag tgaggaagaa ttatttgaac cgcatatgag aaagtgggct gatgaagcga
    21541 ctaaaacagc gacttgggaa gtgtataagt ttattcatgt aaatacggtt gcaccttttg
    21601 gaactttcag aactaaaatc agaaaatgga agaagatagt attataaatt atatttttaa
    21661 ctttaaaaaa tttcataaaa atggttacca aaggcgatag aagaaaaact atcgtctttt
    21721 tctttgcaaa tttttaagaa gggaggtgat cttgcatgga ctttgaatat ttttataaca
    21781 gagaagcgaa aagatttaac ttcttaaaag taccggagat attagttgat agagaagaat
    21841 ttcggggctt atcagcagaa gcaattatcc tttattccat acttcttaaa tagacaggaa
    21901 tgtcatttaa gaataactgg atagacaagg aaggcagagt atttatctat tttactgtcg
    21961 aagaaattat gaaaagaaga aatatctcaa agccaactgc cataaaaaca ttagatgagc
    22021 ttgatataaa aaaaggaata ggactgatcg aaagagtaag gcttggactt ggtaagccga
    22081 acatcattta tgttaaagac tttatgagta tatttcaggt aaaagaaaat gacttacaga
    22141 agtcaaaaaa cttaacttca gaagtaaaag attttaacct cagaagtaaa gaaaatgaac
    22201 ttcaagaggt taagaacctt gactctaact atatagagaa taataagagt aagtatagta
    22261 agagagaata tagttttggt gaaaacggac ttggaacatt tcaaaatgtg tttttagctg
    22321 ctgaagatat ataggattta caaatcataa tgaactcaca gcttgagaat tacattagac
    22381 ttcctgcaaa actagaatcc tagttcatga ttgataatac cagcaatcaa attcattcgt
    22441 aatccgaatc gtttacgatg atttcgatag gttgttgaaa acattttaaa cgtttctact
    22501 ttggcaaaga tgttctcaac cttgcttctc tccttagata gcgcatggtt ataggtttta
    22561 tcttcaactg ttagcggctt gaatttgctg gatttacgtg gagtttgtgc ttgaggatat
    22621 atcttcatga gcccttgata accactgtca gccaagattt taccagcttg tccgatattt
    22681 ctgcgactca ttttgaacaa cttcatatca tgacaatagt tcacagtgat atccaaagaa
    22741 acaattctcc cttgacttgt gacaatcgct tgagccttca tagcgtagca tttcttttta
    22801 ccagaataat tcgctaattc tttttttagg gcgattgatt tttacttccg tcgcatcaat
    22861 aatcattacc gtgtcctcag aactgagagg agttcttgaa atcgtaacac cactttgaac
    22921 aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgaa taccaaaatc
    22981 agccgcaatt tgttcataag ttcgatattc tcgcacatat tgaagagtgg ccataagaag
    23041 gtcttctagg cttaatttag gttttcgtcc accttttgcg tgtttaagtt gataagctgt
    23101 ttttaataca gctaatatct cttcaaaagt cgtgcgctga acaccaacaa gacgcttaaa
    23161 tcgtgcatca gttagttgtt tacttgcttc atcattcata gaactactat accatatttt
    23221 gtttcgcagg aagtctattg gaaagtaaga aatattgaag ctgaggcgat tagaagaaat
    23281 tgtgagcgtg gtgctatttt ttcaggtaaa ataaaacatc acgaagattc tcagtttaaa
    23341 ggagaccact atgttgaatg ttatgctgtt ttagataata cggttatagc aagagataga
    23401 ataacagtcc ctatcgatcc gttatgtgga aaagatttta tagagtagca tataattgat
    23461 tcttaactgg aatactctct atctctttac atcaagaaaa tgactaaaca gggaagtttg
    23521 ccttcttccc tttttttgtt atactagtag aagaaaaaaa tagaaagatt tgtgggagtg
    23581 aaaaagtccg ggggactttt tcagcttgag ccaagaaatt cgagagcgag gaaagtctgg
    23641 gggacttttt cagcttgagc caagaaattc gagagcgagg aaagtctggg ggactttttc
    23701 agcctgagcc aagaaactcg aaatctcgta agtctgattg gctttttcaa tgtgaatctt
    23761 aacttcacac tcccaaagag gtattagtgt cgtgtctcaa tcttatatca atgttatcgg
    23821 tgctggtttg gcaggttctg aagcagctta ccaaatcgca gagcgtggta ttccagttaa
    23881 actttatgaa atgcgtggtg tcaagtctac accccagcat aaaacagaca attttgctga
    23941 gttggtttgt tccaattctt tgcgtgggga tgctttgaca aatgcagttg gtcttctcaa
    24001 ggaagaaatg cgtcgcttgg gttctgttat cttggaatct gctgaggcta cacgtgttcc
    24061 tgccggtgga gcccttgcgg tggatcgtga tggtttctct caaatggtga ccgaaaaagt
    24121 ggtcaaccac cccttgattg aagtggtgcg tgatgaaatt acagaattgc caacagatgt
    24181 tattacagtt gtggcgactg gtcccttgac tagcgatgcc ctggctgaaa agattcatgc
    24241 ccttaataat ggcgatggct tctatttcta cgatgcagca gcgcctatta tcgatgtcaa
    24301 cactatcgat atgagtaagg tctatctcaa atctcgttat gataagggag aagcagccta
    24361 tctcaatgct ccaatgacca agcaagagtt tatggatttc catgaagcct tggtcaatgc
    24421 ggaagaagca ccgctcaatt cttttgaaaa agaaaagtac tttgaaggat gtatgccaat
    24481 cgaagttatg gccaaacgtg gcattaaaac tatgctttat ggccctatga agccagtcgg
    24541 tcttgagtat ccggacgact acacaggacc tcgtgatgga gaatttaaaa caccttatgc
    24601 ggttgtgcaa cttcgtcagg ataatgcagc tggtagccta tacaatattg ttggtttcca
    24661 gacccacctc aaatggggag aacaaaagcg tgtcttccaa atgattccgg gtcttgaaaa
    24721 tgcggagttt gtccgttatg gtgtcatgca tcgcaattct tacatggatt caccaaatct
    24781 tcttgagcag acttaccgtt ccaagaaaca accaaatctc ttctttgctg gtcagatgac
    24841 gggggtggaa ggctatgttg agtcggcggc ttcgggctta gttgcgggaa ttaacgcagc
    24901 tcgtctcttc aaggaagaaa gcgaggttat tttccccgag acgacagcga ttggaagctt
    24961 agctcattac attacccatg ccgacagcaa acatttccaa ccaatgaatg tcaattttgg
    25021 gatcatcaag gagttggaag gcgagcgtat ccgtgataag aaggctcgtt atgaaaaaat
    25081 tgcagagcgt gcccttgccg acttagagga atttttgact gtctaatttt tttgaaagaa
    25141 ttgttcatga tactataaaa atcttagaaa ttgtgataaa ataggtagga tgaaagaagg
    25201 agagtgaaaa tggcgaatcc caagtataaa cgtattttaa tcaagttatc aggtgaagcc
    25261 cttgccggtg aacgtggcgt agggattgat atccaaacag ttcaaacaat cgcaaaagag
    25321 attcaagaag ttcatagctt aggtatcgaa attgcccttg ttattggtgg aggaaatctc
    25381 tggcgtggag aacctgcagc agaagcaggt atggaccgtg ttcaggcaga ttacactgga
    25441 atgcttggga ctgttatgaa tgctcttgtg atggcagatt cattgcaaca agttggggtt
    25501 gatacgcgtg tacaaacagc tattgctatg caacaagtgg cagagcctta tgtccgtgga
    25561 cgtgcccttc gtcaccttga aaaaggccgt atcgttatct ttggtgctgg aattggttca
    25621 ccatacttct cgacagatac aacagcggcc cttcgtgcag ctgaaatcga agcagatgcc
    25681 atcctcatgg ctaaaaatgg cgtcgatggt gtgtacaatg ccgatcctaa gaaggacaag
    25741 acagccgtta agtttgaaga attgacccac cgtgatgtta tcaacaaagg tcttcgtatc
    25801 atggactcaa cagcctcaac cctctcaatg gacaacgaca ttgacttggt tgtcttcaac
    25861 atgaaccaat caggcaacat caaacgtgtt gtatttggtg aaaatatcgg aacaacagtt
    25921 tcaaataata tcgaagaaaa ggaataagaa agaatatggc taacgtaatt attgaaaaag
    25981 ctaaagagag aatgacccag tctcaccaat cacttgctcg tgaatttggt ggtatccgtg
    26041 ctggtcgtgc caatgcaagc ttgcttgacc gtgtacatgt agaatactat ggagtcgaaa
    26101 ctcctcttaa ccaaatcgct tcaattacga ttccagaagc gcgtgttttg ttggtaacac
    26161 catttgacaa gtcttcattg aaagacatcg aacgtgcctt gaacgcttct gatcttggta
    26221 tcacaccggc taatgacggt tctgtgattc gcttggttat cccagctctt acagaagaaa
    26281 ctcgtcgtga ccttgctaaa gaagtgaaga aggtcggcga aaatgctaaa gtggctgtcc
    26341 gcaatatccg tcgcgatgct atggacgaag ctaagaagca agaaaaagca aaagaaatca
    26401 ctgaagacga attgaagact cttgaaaaag atattcaaaa agtaacagac gatgctgtta
    26461 aacacatcga cgacatgact gctaacaaag agaaagaact tttggaagtc taaaaataaa
    26521 cagaaaaact cagttggcat tgctggctga gttttattcg aaagaaggaa atatgaatac
    26581 aaatcttgca agttttatcg ttggactgat catcgatgaa aatgaccgtt tttactttgt
    26641 gcaaaaggat ggtcaaacct atgctcttgc taaggaagag ggccaacata cagtagggga
    26701 tacggtcaaa ggttttgcat acacggatat gaagcaaaaa ctccgcctga caaccttaga
    26761 agtgactgcc actcaggacc aatttggttg gggacgtgtc acagaggttc gtaaggactt
    26821 gggtgtcttt gtggatacag gccttcctga caaggaaatc gttgtgtcac tcgatattct
    26881 ccctgtgctc aaggaactct ggcctaagaa gggcgaccaa ctctacatcc gtcttgaagt
    26941 ggataagaaa gaccgtatct ggggcctctt ggcttatcaa gaagacttcc aacgtctcgc
    27001 tcgtcctgcc tacaacaaca tgcagaacca aaactggcca gccattgttt accgtctcaa
    27061 gctgtcagga acttttgttt acctaccaga aaataatatg cttggtttta ttcatcctag
    27121 cgagcgttac gcagagccac gtttggggca agtattagat gcgcgcgtta ttggtttccg
    27181 tgaagtggac cgcactctga acctctccct caaaccacgc tcctttgaaa tgttggaaaa
    27241 cgatgctcag atgattttga cttatttgga aagtaatggc ggtttcatga ccttaaatga
    27301 taagtcatct ccagacgaca tcaaggcaac ctttggcatt tctaaaggtc agttcaagaa
    27361 agctttaggt ggtcttatga aggctggtaa aatcaagcag gaccagtttg ggacagagtt
    27421 gatttaggga ggcttatgag aaaatcattt tacacttggc tcatgaccga gcgcaatcct
    27481 aaaagtaaca gtcccaaagc aattttggca gacctcgctt ttgaagaggc agcttttcca
    27541 aaacatacag atgattttga tgaggtcagt cgctttttgg aggagcatgc cagtttctct
    27601 tttaacctag gagattttga cagcatttgg caggaatatc tagaacacta gcatttattc
    27661 attgggtttg ggctagtaat ttctccatcc ctctgctata ataaaaagaa ataaaaggat
    27721 tagagaggtt ctttatttga aggaacattc aatagacatt caactgagtc atccagatga
    27781 cctgtttcat ctttttggtt ccaatgaacg ccatcttcgt ttgatggaag aagagcttga
    27841 tgttgtgatt catgctcgta cggagattgt ccaggttttg ggagaagagt ctgcctgtga
    27901 ggaagcccgt caagttattc aggctttgat ggtcttggta aatcgtggga tgaccgttgg
    27961 tacgccagat gtagtcactg cgattagcat ggtcaaaaat gatgaaattg acaagtttgt
    28021 cgccctttac gaagaagaaa ttatcaaaga taatactggg aaacctatcc gtgtcaaaac
    28081 cctagggcaa aagctttatg tggacagtgt caaacagcat gatgtgacct ttggaattgg
    28141 gccagcaggt acagggaaga ccttccttgc agtgaccttg gcagtgactg cccttaaacg
    28201 tgggcaagtc aagcgaatta tcctaactcg tccagcggtg gaagcgggag agagtcttgg
    28261 atttcttccg ggtgatctta aggagaaggt ggatccttac cttcgtcctg tttacgatgc
    28321 cttgtatcag attctgggga aagaccaaac aactcgactc atggagcgtg aaattatcga
    28381 gattgcgccc cttgcctata tgcgtggacg gaccttggat gatgcctttg tcattctcga
    28441 tgaggcgcaa aatacgacca tcatgcagat gaagatgttc ttgactcgtt taggttttca
    28501 ttctaagatg attgtcaatg gagatattag tcagattgac ctgccacgta atgtcaagtc
    28561 cggtttgatt gatgctcaag agaaactcaa gaacatccat cagattgact ttgttcattt
    28621 ttcagccaag gatgtggttc gccatccagt tgtcgctcag attatccgag cctatgaata
    28681 ttctactgaa gttgcacacg actgattttg aggaagtttg cctgcaaaag aatatacttt
    28741 tgagagagga aaatccagtt gtataggcta aaggttttat ccaaaggttt gagacaacga
    28801 ttaggcacga tggaaagaac ttttatgtgg ctgatgacga tcagtgcatc ttcctgtgtc
    28861 ataatcacag ggcacaagaa agtaggaatt tgaaaagatg attgaccaac tatctaagta
    28921 ttacagttgt aggatactaa ctgaaaagga tattccaagt attttatctt tatatgaaag
    28981 taatcctctg tattttcagc attgtccacc agagccaaat tttgcaactg taaaagagga
    29041 catgctttgt ctacctgaag gtaaagctaa ggctgataag ttttttgttg gattttggaa
    29101 tggatctgac cttgtggctg ttatagattt tgtctatgca catcctgatg aggagactgt
    29161 ttttattggt ttgtttatgg ttgatcaagc ctatcagagg aaagggattg gtagtcatat
    29221 tgtgacagaa gcactagctt attttgctaa gaactttcga aaggcacgtt tggcttatgt
    29281 taagggaaat ccgcaatctc agcatttttg ggaaaagcag ggctttaaat caattggatg
    29341 cgaggttaag caagaactct atacggttgt tatcgctgaa cagagcctag aagattagaa
    29401 atggcatcaa gtaagaacta tttggaattt gttttggaac aattatcagg attagatgat
    29461 gtgacttacc gttccatgat tggggagtat attctttact tccgcggcaa gattattggc
    29521 ggcatttatg acgatcgctt tttagttaaa cccgtgcaag cagtcttaga taagattgac
    29581 caatcttctt ttgagtttcc atacaaaggt gccaaagaaa tgatttgagt ggaagaactt
    29641 gataataaga tgtttctata agacctgatt ttagctatgt ataaccaact gccaacgccc
    29701 aaacctaaaa agaaaaagca agggtgaacg aagtaaaaaa gaagtctgct aaggccctgt
    29761 ctttgcacgg gtaaaatttt atatataaaa agaagctggg actaaagagc tcagcttcct
    29821 ttggtttata taattgtcat tacaagacga agtggttggg cgaaactctg ttgactttat
    29881 tcaatttaga gtttcttatg cacaattgag tctggaacga aagtctccag ttgcaaagta
    29941 tacagtacaa taaaccaacg atgtaatagc tgatgacaca aagcacagtg ggtaggactt
    30001 gcgaagtcac ccttttcttt tcaaaattta tactaaatca ttgatatcag tgtagtcacg
    30061 attaagtcct tgagcaactg gtaggctagt caagtaacct tgataagtgg tcacaccttg
    30121 acgcaagcct tcatcttcag agattgcttg tgcgaatcct ttgccagcca aagcttcgat
    30181 ataaggaaga gtgacattgg ttagggcgat ggttgaagtg cgggcaaccg caccagggat
    30241 attggcaacg gcatagtgga gaacaccgtg tttttcatag acgggttcat cgtgcgttgt
    30301 cacacggtca gctgtttcga taacgccacc ttggtcaaca gcaacgtcaa cgatacagag
    30361 cctggacgca tttgtttgac catctcatct gtcaccaatt ccggtgcttt tgcaccaggg
    30421 atgagaatgg ctccaatcac cacatcagca tctctcatac ttgcttcaat gttgaatgaa
    30481 ttagatataa gagtttgaat ttgacttcca aagacttctt ctagaactga gagacgcttg
    30541 gcactaatat ctaaaatagt cacttgagca ccaagaccaa gggcgatgcg ggcagcatgt
    30601 gtaccgacga caccatcacc gatgatagtt acttttcctt ttggaacacc tggtacacca
    30661 ccaggtagaa caccagagcc accagcttgc ttagtaagga agtgagctcc gatttaaaca
    30721 gccatacgac ctgcaacctc actcatagga acgaggagcg gtagttgtcc ttgattgtca
    30781 cgaacagttt cataggcaat tcctgttgtt tttgctgcta acatagcatc tgctaattct
    30841 ggagcagcgg ccatgtgcaa gtaggtgaag agaagaagat cgtcgcgcaa gtaaccgtat
    30901 tcagaactta aaggttcttt tactttcaca accaactctg ctgcccaagc ttcaccagca
    30961 gtagcgacaa tctcagctcc ttgcttttga tagtcagcat cagtaaagcc agaaccgaga
    31021 ccagcatttg tttcgataag gacacgatga ccacgactaa ctaagctatg aacacctgca
    31081 ggtgtgaggg cgacacggtt ttcgttattt ttaatttctt ttgggattcc gattaacatt
    31141 gagataacct acctttcaat tgacggtctt gttttggttg tcacattcca gttcataaat
    31201 caaaaatgtg acggtttcat tgtatatgaa accgcttcaa aaatcaagaa aaacttgtca
    31261 tccaaatttt tttatgctag actagtgaaa atcaagctct aatggaggga aaagtatgga
    31321 atcaatattt gtgaaatttg cccagtatcc gtctatagaa acggagcgtt tattgcttag
    31381 acctgtaact ttggatgatg cggaagcaat gtttgactat gcctcggaca agggtaatac
    31441 acgttacact tttccaacca atcaaagctt ggaagaaacc aagaataaca ttgctcagtt
    31501 ctacttggct aatcccttgg gacgttgggg gattgaatta aaagataacg gcaagtttat
    31561 cggaactatt gacttacaca agattgatcc tgttcttaag aaggcagcta ttggctacat
    31621 tatcaataaa aagtattgga atcaaggatt aacgccagaa accaatcgtg ctgtgattga
    31681 gctagctttt gagaagatag gaatgaataa gttagttgct ttatacgata aggacaattc
    31741 tgcgtcagga aaggtcatgg agaaatcagg catgcgtttt tcccatgcag aaccatatgc
    31801 ttgtatggac cagcatgaaa aaggccgaat cgtgacaaga gttcattatg tcttgaccaa
    31861 ggaagactat tttgcaaata aataagcagt tgaaaagaaa tttttcgact gttttttctt
    31921 cctcttacga ataatctaag agaggagaaa atatggaagc aattatcgag aaaatcaaag
    31981 agtataaaat catcgtcatc tgtactggtc tgggcttgct tgtaggcgga tttttcctgc
    32041 taaagccagc tccacaaaca cctgtaaagg aaacgaattt gcaggctgaa gttgcagctg
    32101 tttccaagga tttggtatcc gaaaaggaag tgaacaagga agagaaggaa gaaccccttg
    32161 aacaagatct aatcacagta gatgtcaaag gtgctgtcaa atcgccaggg atttatgact
    32221 tgcctgtagg tagtcgagtc aatgatgctg ttcagaaggc tggtggcttg acagagcaag
    32281 cagacagcaa gtcgctcaat ctagctcaga aagttagtga tgaggctctg gtttacgttc
    32341 ctactaaggg agaagaagca gttagtcaac agactggttc ggggacagct tcttcaataa
    32401 gcaaggaaaa gaaggtcaat ctcaacaagg ccagtctgga agaactcaag caggtcaagg
    32461 gactgggagg aaaacgagct caggacatta tcgaccatcg tgaggcaaat ggcaagttca
    32521 agtcagtaga cgagctcaag aaggtctctg gcattggtgg caaaacaata gaaaagctta
    32581 aagactatgt tacagtggat taagaatttc tctattcccc taatttacct gagttttcta
    32641 ttactttggc tttattacgc tattttctca gcatcttatc ttgctttgtt gggctttgtt
    32701 tttctgctag tctgtctctt tatccaattt ccgtggaaat ctgctggtaa agttctaata
    32761 atttgcggaa tctttggatt ttggtttgtt tttcaaaatt ggcaacagag tcaagcgagt
    32821 caaaatctgg cggattctgt tgaaagggta cggattttgc ctgatactat taaggttaat
    32881 ggtgatagtc tatcctttcg tggcaagtct aacggtcgtg ctttccaagt ctattataaa
    32941 ctccagtccg aggaggagaa agaagccttt caagctttaa ccgacctgca tgagatagga
    33001 ctagaaggga agctttcgga gccagaaggg cagagaaatt ttggtggctt taattaccaa
    33061 gcctatctga agactcaggg aatttaccag actctcaata tcaaaaaaat ccagtcactt
    33121 caaaagattg gcagttggga tataggagaa aacttgtcca gtttacgtcg aaaggctgtg
    33181 gtttggatta agacgcactt tccagaccct atgcgcaatt acatgacagg actcttgctg
    33241 ggacatctgg acaccgactt tgaggagatg aatgagcttt attccagtct aggaattatc
    33301 cacctctttg ccctatctgg tatgcaggta ggttttttca tgaatggatt taagaaactt
    33361 ctcttgcgat tgggcttgac ccaagaaaag ttgaaatggc tgacttatcc cttttccctt
    33421 atctatgcgg gactaactgg attttcagca tcggttattc gcagtctctt gcaaaagcta
    33481 ctggctcaac atggggttaa gggcttggat aattttgcct tgacggtgct tgtcctcttt
    33541 attgtcatgc caaacttttt cttgacagca ggaggagtct tgtcctgcgc ttatgctttt
    33601 atcctgacca tgaccagcaa agaaggggag gggctcaagg ctgttactag tgaaagtcta
    33661 gtcatctcct tgggcatatt gcccattcta tccttctatt ttgcggaatt tcaacctttg
    33721 tctatccttt tgacctttgt cttttccttt ctatttgact tgaccttctt accgctcttg
    33781 tctattttat ttgtcctttc ctttctctat ccagtcattc agctgaactt tatctttgaa
    33841 tggttggagg gcattattcg cttggtgtca caggtgacaa gtagacctct ggtctttgga
    33901 caacccaatg catggttttt aatcctattg ttaatttcct tggctttggt ctatgatttg
    33961 agaaaaaaca ttaaaaagct aacggtattg tgcttattga ttacagggct ctttctcctg
    34021 accaagcatc cactggaaaa tgaaatcacc atgctggatg tggggcaagg agaaagtatt
    34081 ttcctacggg atgtaactgg gaaaaccatt ctcatagatg taggtggtaa ggcagaatct
    34141 tataagaaaa tcgaaaaatg gcaagaaaaa atgacgacca gcaatgccca gcgaaccttg
    34201 attccctatc tcaaaagtcg aggagtagct aagattgacc agctaatttt gactaacacg
    34261 gacaaggaac atgtcggaga tttgttagag gtgaccaagg ctttccatgt aggggagatt
    34321 ttagtatcaa aaggcagttt gaagcagaag caatttgtgg tagaactaca ggcgactcaa
    34381 acaaaggtgc gtagtatgac agtaggggag aacttgccca tttttggaag tcagttagaa
    34441 gttctatccc caaggaaaat gggagatgga ggacacgatg ataccctagt tctgtatggg
    34501 aaattcttgg ataagcaatt tctcttcacg ggaaatttgg aggagaaagg agagaaggac
    34561 ttgctgaagc actatccaga cttgaaagta aatgttttga aagctagcca acatggcaat
    34621 aaaaaatcat caagtccagc ctttctagaa aaactcaaac cagagcttac tcttatctca
    34681 gttggaaaga gcaatcgaat gaaactcccc catcaggaaa cattgacacg actggaaggt
    34741 atcaatagca aagtttaccg aactgaccag caaggagcta tacggtttaa aggttggaat
    34801 agttggaaaa tcgaaagtgt tcgataggaa agataaatgt tgtagattag tgaaataaac
    34861 taaaaatttg ttgcataata atgataaaaa tggtataatg aaaacgtatt caatactgag
    34921 gatataaaat cattaaaaat tagcaaaaat cgtttaatta gttagtttat gattattggt
    34981 ctttttcagt ccagtatatc tgctgtgaca gtcactaaaa gttacaagta tgattggaat
    35041 acggtttggg aatatagtac taactatcac gaccatcagt atgcttggat tccgtcatgg
    35101 tctcgttatt acagctattc tgagtatcca gttggttctg gctggaacta cgctcgttat
    35161 gaggtcataa actactatac tggaggttat taatccttaa agagtgagaa aaggagggct
    35221 ggatatgtta aggcttactc atgttaccct aaaaacgcga caagtcatct tgcaagatgc
    35281 ggattttacc tttaaaaagg gtagggttta tggtcttctt gctatcaatg gctcgggaaa
    35341 gacgacacta ttccgagcta tgagcaagtt gcttcccctt agtagtggac acatcgcagt
    35401 tcctccttct ttgttttatt atgagagcgt tgaatggctg gatggaaact taagtgggat
    35461 ggactacctt cgtctcataa aaaacatctg gaagtcagac ctaaacttga gagatgaaat
    35521 cgcctactgg gaaatggctg actatatcag tcttcccatc cgcaagtatt ccttaggcat
    35581 gaagcaacgc ttggtgattg ctatgtattt tctcagtcag gccaaatgct ggctcatgga
    35641 tgagattaca aatggcttag acgagtatta tcgacagaag ttttttgata ggctagcaca
    35701 aatcgataga caagaacagc tggttctttt aagttcccac tataaggaag agttggttga
    35761 tatctgcgat agagtagtaa ccattcatca ggggcagata gaagaggttt agtttatgaa
    35821 agatgttagt ctatttttat tgaaaaaagt tttcaaaagt cgtttaaact ggattatctt
    35881 acttttattt gcatctgtac tcggtgttac cttttattta aatagtcaga ctgcaaactc
    35941 acacagcttg gagagcaggt tggaaagtcg cattgcagcc aacgagaggg ctatcaatga
    36001 aaatgaagag aaactctccc aaatgtctga taccagctcg gaggaatacc agtttgctaa
    36061 aaataattta gacgtgcaaa aaaatctttt gacgcgaaag acagaaattc taactttact
    36121 aaaagaaggg cgctggaaag aagcctacta tctgcagtgg caagatgaag agaagaatta
    36181 tgaatttgta tcaaatgacc cgactgctag ctctggctta aaaatggggg ttgaccgcga
    36241 acggaagatt taccaagccc tgtatccctt gaacataaaa gcacatactt tggagtttcc
    36301 gacccacggg attgatcaga ttgtctggat tttagaggtt atcatcccaa gcttgtttgt
    36361 ggttgcgatt atttttatgc taacacaact atttgcagaa agatatcaaa atcatctgga
    36421 cacagctcac ttatatcctg tttcaaaagt gacatttgca atatcctctc ttggagttgt
    36481 agtgggatat gtaactgtgc tgtttatcgg aatctgtggc ttttcttttc tagtgggaag
    36541 tctgataagt ggttttggac agttagatta tccctaccca atttatagct tagtgaatca
    36601 agaagtaact attgggaaaa tacaagatgt attatttcct ggcttgctct tagctttctt
    36661 agcctttatc gtcattgtgg aagtcgtcta cttgattgct tactttttca agcaaaaaat
    36721 gcctgtcctc tttctttcac tcattgggat tgttggctta ttgtttggca tccaaacaat
    36781 tcagcctctt caaaggattg cacatctgat tccctttact tacttgcgtt cagtggagat
    36841 tttatctgga agattatcta agcagattga taatgtcgat ctaaattgga gcatggggat
    36901 ggtcttactt ccttgcctga ttatcctttt gttagtgggg attctattta tcgaaagctg
    36961 gggaagttca cgtaaaaagg aagtttttaa agtttagttt tcctataggg aagctaaaga
    37021 aaaactaaca tagggagaga atcaacttgg ttctctcttt ttgattcgaa aaccaaacca
    37081 aaatacaaac acaaactttt caaaaaataa ctttttatct tgacaagagc tagaaaactt
    37141 ggtatcatat aaaagttgag aaaagcagaa gtgagagctt ctcgccttgt gacattaagt
    37201 tgcctggccc tacggatgaa aagtttcgaa gaaacgctat cataacgtgc gggcttgtat
    37261 atttacaagt ccgctattgt ttttctctaa taaaacaaaa gaggtgaaaa ccatagcaaa
    37321 gcaagactta ttcatcaatg atgagattcg tgtacgtgaa gttcgcttga ttggtcttga
    37381 aggagaacag ctaggtatca agccactcag tgaagcgcaa gctttggctg ataacgctaa
    37441 tgttgaccta gtattgattc aaccccaagc caaaccgcct gttgcaaaaa ttatggacta
    37501 cggtaagttc aaatttgagt accagaagaa gcaaaaagaa caacgtaaaa aacaaagcgt
    37561 tgttactgtg aaagaagttc gtctaagtcc aactattgac aagggtgact ttgatacaaa
    37621 acttcgtaat gcacgtaagt tccttgaaaa aggaaataaa gttaaggtat ctattcgctt
    37681 taagggtcgt atgattaccc ataaagagat tggtgcaaaa gttttagccg agtttgctga
    37741 agcaactcaa gatattgcca tcatcgaaca acgtgctaaa atggatggac gtcaaatgtt
    37801 catgcaattg gcgccagcga ctgacaaaaa ataattgtca gaaagttaaa taaaggagaa
    37861 aaaatcatgc caaaacaaaa aacacaccgc gcatcagcta aacgtttcaa acgtacaggt
    37921 tctggtggac ttaaacgttt ccgtgcttac acttctcacc gtttccacgg aaaaactaag
    37981 aaacaacgtc gtcatcttcg taaagcatct atggtgcatt caggagatta caaacgtatc
    38041 aaagcaatgc ttactcgctt gaaataatag cttgactgta agtaaacaat tctaggaaat
    38101 atttggagga aataaaacat ggcacgtgtt aaaggtggcg ttgtatcacg caaacgtcgt
    38161 aaacgtattc ttaaattagc aaaaggttac tatggagcta aacacatctt gttccgtact
    38221 gcaaaagaac aagtaatgaa ctcttactac tatgcatacc gtgaccgtcg tcagaaaaaa
    38281 cgtgacttcc gcaaattgtg gattactcgt atcaatgcgg cagctcgtat gaacggactt
    38341 tcatactcac aattgatgca tggtttgaaa ttggctgaga ttgaagttaa ccgtaaaatg
    38401 cttgctgact tggctgttaa cgatgcagta gctttcacag ctcttgcaga tgcagctaaa
    38461 gcaaaacttg gtaaataaca actgatgaga ctggaacagg attgtccagt ctttttaaaa
    38521 ataaaggaga aagatatggc ttcaaaaatg ctacacactt gcttacgagt agaaaatctt
    38581 gaaaaatcaa tcgcatttta tcaagatgct tttggtttta aagaattgcg tcgcagagat
    38641 tttccagacc atgccttcac gattgtctat ctaggtcttg agggtgatga ttatgagttg
    38701 gagttgactt ataactacga tcacggtccg tatgtggttg gtgatgggtt tgcccatatc
    38761 gccctcagta cacctgatct tgaggcactt catcaagaac acagtgcaaa aggctatgaa
    38821 gtgactgagc caaatggtct accaggaact acaccaaact attactttgt taaagaccct
    38881 gatggatata aggtcgaagt cattcgtgaa aaataatttc gtgcaagtta agtagaatat
    38941 tcgttttcta cttgactttt tttagctgaa agagtatact aataaaaatt caacctttaa
    39001 ggggagtcca gagagactca caaggtgtca gataaaagaa tagtgcaatt ttctagagga
    39061 gaccttttga gtgtgctctc ttgtgttgta cgattttaac tgaggccttg cactagcaag
    39121 gtcttttctt tatctggtcc ccttaaaatt taaggaggaa aagttatgaa tctcacatgt
    39181 aagaagcgtt tgggtgtcat tcggttggaa accatgaagg tggttgcaca agaggaaatc
    39241 gcgccagcaa tctttgaatt agtcctagaa ggagaaatgg ttgaagccat gcgagcaggc
    39301 caatttcttc atctgcgtgt accagacgat gcccatctct tacgtcgtcc tatttcaatt
    39361 tcgtctattg acaaggcaaa caagcagtgt cacctcattt atcggattga gggagctggg
    39421 actgcaattt tttcaacctt aagtcaggga gacactcttg atgtgatggg gcctcaggga
    39481 aatggttttg acttgtctga ccttgatgag cagaatcagg ttctccttgt tggtggtggg
    39541 attggtgttc cacccttgct tgaggtggcc aaggaattgc atgaacgtgg agtgaaagta
    39601 gtgacagtcc tcggttttgc taataaggat gctgttattt tgaaaacgga attggctcag
    39661 tatggtcagg tctttgtaac gacagatgat ggttcttatg gcatcaaggg aaatgtttcc
    39721 gttgttatca atgatttaga tagtcagttt gatgctgttt actcgtgtgg ggctccagga
    39781 atgatgaagt atatcaatca aacctttgat gatcacccaa gagcctattt atctctggaa
    39841 tctcgtatgg cttgtgggat gggagcttgc tatgcctgtg ttctaaaagt accagaaagc
    39901 gagacggtca gccaacgcgt ctgtgaagat ggtcctgttt tccgcacagg aacagttgta
    39961 ttataaggag aaaattatga ctacaaatcg attacaagtg tctctacctg gtttggattt
    40021 gaaaaatccg attattccag catcaggctg ttttggcttt ggacaagagt atgccaagta
    40081 ctatgattta gaccttttag gttctattat gatcaaggcg acaacccttg aaccacgttt
    40141 tgggaatcca actccaagag tggcagagac gcctgctggt atgctcaatg caattggctt
    40201 gcaaaatcct ggtttagagg ttgttttggc tgaaaagcta ccttggctgg aaagagaata
    40261 tccaaatctt cctattattg ccaatgtagc tggtttttca aaacaagagt atgcagctgt
    40321 ttctcatggg atttccaagg caactaatgt aaaagctatc gagctcaata tttcttgtcc
    40381 caatgttgac cactgtaatc atggactttt gattggtcaa gatccagatt tggcttatga
    40441 tgtggtgaaa gcagctgtgg aagcctcaga agtgccagtt tatgtcaaat taaccccgag
    40501 tgtgaccgat atcgttactg tcgcaaaagc tgcagaagat gcgggagcaa gtggcttgac
    40561 tatgatcaat actctggttg gaatgcgctt tgacctcaaa accagaaaac caatcttggc
    40621 caatggaaca ggtggaatgt caggtccagc agtctttcca gtagccctca aactcatccg
    40681 ccaagtagcc caaacaacag acctgcctat cattggaatg gggggagtgg attcggctga
    40741 agctgcccta gaaatgtatc tggctggggc atctgctatc ggagttggaa cagctaactt
    40801 taccaatcct tatgcctgcc ctgacatcat cgaaaattta ccaaaagtca tggataaata
    40861 cggtattagc agtctggaag aactccgtca ggaagtaaaa gagtctctga ggtaaactgc
    40921 aatcaatctg ttcttgattt tttattagtt tgtaatatga atttaggaga attttggtac
    40981 aataaaataa ataagaacag aggaagaagg ttaatgaaga aagtaagatt tattttttta
    41041 gctctgctat ttttcttagc tagtccagag ggtgcaatgg ctagtgatgg tacttggcaa
    41101 ggaaaacagt atctgaaaga agatggcagt caagcagcga atgagtgggt ttttgatact
    41161 cattatcaat cttggttcta tataaaagca gatgctaact atgctgaaaa tgaatggcta
    41221 aagcaaggtg acgactattt ttacctcaaa tctggtggct atatggccaa atcagaatgg
    41281 gtagaagaca agggagcctt ttattatctt gaccaagatg gaaagatgaa acgaaatgct
    41341 tgggtaggaa cttcctatgt tggtgcaaca ggtgccaaag taatagaaga ctgggtctat
    41401 gattctcaat acgatgcttg gttttatatc aaagcagatg gacagcacgc agagaaagaa
    41461 tggctccaaa ttaaagggaa ggactattat ttcaaatccg gtggttatct actgacaagt
    41521 cagtggatta atcaagctta tgtgaatgct agtggtgcca aagtacagca aggttggctt
    41581 tttgacaaac aataccaatc ttggttttac ataaaagaaa atggaaacta tgctgataaa
    41641 gaatggattt tcgagaatgg tcactattat tatctaaaat ccggtggcta catggcagcc
    41701 aatgaatgga tttgggataa ggaatcttgg ttttacctca agtttgatgg caaaatggct
    41761 gaaaaagaat gggtctacga ttctcatagt caagcctggt actacttcaa atccggtggt
    41821 tacatgacag ccaatgaatg gatttgggat aaggaatctt ggttttatct caaatctgat
    41881 gggaaaatag ctgaaaaaga atgggtctac gattctcata gtcaagcttg gtactacttc
    41941 aaatccggtg gttacatgac agccaatgaa tggatttggg ataaggaatc ttggttttac
    42001 ctcaagtctg atggcaaaat ggctgaaaaa gaatgggtct acgattctca tagtcaagcc
    42061 tggtactact tcaaatctgg tggctacatg gcgaaaaatg agacagtaga tggttatcag
    42121 cttggaagcg atggtaaatg gcttggagga aaagctacaa ataaaaatgc tgcttactat
    42181 caagtagtgc ctgtcacagc caatgtttat gattcagatg gtgaaaagct ttcctatata
    42241 tcgcaaggta gtgtcgtatg gctagataag gatagaaaaa gtgatgacaa gcgcttggct
    42301 attactattt ctggtttgtc aggctatatg aaaacagaag atttacaagc gctagatgct
    42361 agtaaggact ttatccctta ttatgagagt gatggccacc gtttttatca ctatgtggct
    42421 cagaatgcta gtatcccagt agcttctcat ctttctgata tggaagtagg caagaaatat
    42481 tattcggcag atggcctgca ttttgatggt tttaagcttg agaatccctt ccttttcaaa
    42541 gatttaacag aggctacaaa ctacagtgct gaagaattgg ataaggtatt tagtttgcta
    42601 aacattaata atagcctttt ggagaacaag ggcgctactt ttaaggaagc cgaagaacat
    42661 tatcatatca atgcccttta tctccttgcc catagtgccc tagaaagtaa ctggggaaga
    42721 agtaaaattg ccaaagataa gaataatttc tttggcatta cagcctatga tacgacccct
    42781 tacctttctg ccaagacatt tgatgatgtg gataagggaa ttttaggtgc aaccaagtgg
    42841 attaaggaaa attatatcga taggggaaga actttccttg gaaacaaggc ttctggtatg
    42901 aatgtggaat atgcttcaga cccttattgg ggcgaaaaaa ttgctagtgt gatgatgaaa
    42961 atcaatgaga agctaggtgg caaagattag tactataagt gaatatgatt tgagtgaata
    43021 gtaagttaaa aatcctgatt tcaagtaaaa tcaggatttt ttcatggatg caattttttt
    43081 ggagtctggt gtgacgcgga gggtcttttg tcctgtgtaa gtgacaaagc cgggttttcc
    43141 accagttggt ttattgagtt ttttgacttc aatcatatct acctgcacca gattcgacag
    43201 gcgcccttga gagaagtagg cagctaactc tgctgcgtct gtcttgactg catcagatgg
    43261 gtcaagattt cctgagatga caacatggct tccaggaatg tccttagcat ggaaccaaag
    43321 ttcctccttg cgggccattt taaaggtcaa ttcctcattt tgaagattgt ttcgtccgac
    43381 atagatgatg gttttgccat cgcttgctag atattgttct agttttttgc gtttctggat
    43441 tttctcccgt tgtcttctgc ggataaaacc tgtttgaatc aattcttcac ggatttcagc
    43501 gatttcttcc agtccagctt ggttgaggac ggtttctaca ctttccagat agagaatagt
    43561 ggctttggtt tcttcaatca aatcagtcaa gtatttgaca gcttctttga gtttctgata
    43621 ccgtttaaaa tagcgttggg cattctggtt gggagtcaga gccttatcaa gcgcaatcat
    43681 gataggttgg ttggtatagt agttgtctag gataacctgg tcttggtcgt taggcacttg
    43741 gtgtaggaag gttgtcagca attctccttt ttgacgaaat tcttcagcgt tgtctgtcgc
    43801 cagtaactct ttttcctgtt ttttgagttt gtgtcggttt ttctgaagtt cattttcaac
    43861 acgacgaatc agttcactgg cctgctgttt gacgcggtcg cgctcagcct tatccttata
    43921 gtaggtgtcc aacaaatcag aaagatttgc aaaaggctct cccacctgat ttgcaaaagg
    43981 aactggactg aaggaagtct cagtcaagca tggcttggtt tcttgattga aaaaatttcg
    44041 gaaagcggaa agtttttcac taaccagtat cctttccaat tcatttgcag tatcgcgtcc
    44101 cagaccttga aagaggcttt gaagattttt tgctgttagt tcttgggttt gcaggatttc
    44161 aaagagcttt tcatctttga tagtaaaagg attgagagat tctgtacttg gcggagcgat
    44221 ataggtcgat cctggaagta aggtgcggta gctattttgt gaaaaaccga cgtgtttgat
    44281 aacttcgagg attttatgac tgcttttatc gaccagtaga atattactgt gtttccccat
    44341 aatttcaata atcaaggtag cctggatatg gtctccaatc tcgtttttat tggaaactgt
    44401 aatttccaca atacggtcat tttccacttg ctcaatcgac tcaatcaggg ccccctgcaa
    44461 atactttctc aaaaccatga taaaggtaga aggttgagct ggattttcaa aagtcgtttg
    44521 ggtcagctga atgcgtccaa aaactggatg ggcagaaagg agcaggcgat ggctttggcg
    44581 attgctgcgg atttgcaaga ccaactcttg ttcaaaaggc tgattgattt tctggatgcg
    44641 accattcact aattcgcttc gcaattcctc aactatgtgg tgtaaaaaaa atccgtcaaa
    44701 tgacatcgtt ctctccttgt gattgtattc catagtatta tatcaaaaag gtagaataaa
    44761 atcatggaaa tgtggtataa taaagccaag taaagagaaa cgagaagcac atgtatattg
    44821 aaatggtaga tgaaactggt caagtttcaa aagaaatgtt gcaacaaacc caagaaattt
    44881 tggaatttgc agcccaaaaa ttaggaaaag aagacaagga gatggcagtc acttttgtga
    44941 ccaatgagcg tagtcatgaa cttaatctgg agtaccgtga caccgaccgt ccgacagatg
    45001 tcatcagcct tgagtataaa ccagaattgg aaattgcctt tgacgaagag gatttgcttg
    45061 aaaatccaga attggcagag atgatgtctg agtttgatgc ctatattggg gaattgttca
    45121 tctctatcga taaggctcat gagcaggccg aagaatatgg tcacagcttt gagcgtgaga
    45181 tgggcttctt ggcagtacac ggctttttac atattaacgg ctatgatcac tatactccgg
    45241 aagaagaagc ggagatgttc ggtttacaag aagaaatttt gacagcctat ggactcacaa
    45301 gacaataaac gaaaatggaa aaatcgtgac ttgatatcca gtttagaatt tgccatcaca
    45361 gggattttta ctgctatcaa ggaagaacga aatatgcgaa aacatgcagt gacggctcta
    45421 gtggtcatcc ttgcaggttt tgtttttcag gtgtcacgaa tcgaatggct ctttcttcta
    45481 ttgagtattt tcttggtagt agcctttgag attatcaact ctgctattga aaatgtggtg
    45541 gatttggcca gtcactatca cttttccatg ctggctaaaa atgccaagga tatggcggcc
    45601 ggcgcggtat tagtggtttc tctttttgca gccttaacag gcgcattgat ttttctccca
    45661 cgaatctggg atttattatt ttaaacagta agaggaaatt atgactttta aatcaggctt
    45721 tgtagccatt ttaggacgtc ccaatgttgg gaagtcaacc tttttaaatc acgttatggg
    45781 gcaaaagatt gccatcatga gtgacaaggc gcagacaacg cgcaataaaa tcatgggaat
    45841 ttacacgact gataaggagc aaattgtctt tatcgacaca ccagggattc acaaacctaa
    45901 aacagctctc ggagatttca tggttgagtc tgcctacagt acccttcgcg aagtggatac
    45961 cgttcttttc atggtgcctg ctgatgaagc gcgtggtaag ggggacgata tgattatcga
    46021 gcgtctcaag gctgccaagg ttcctgtgat tttggtggtg aataaaatcg ataaggtcca
    46081 tccagaccag ctcttgtctc agattgatga cttccgtaat caaatggact ttaaggaaat
    46141 tgttccaatc tcagcccttc agggaaataa cgtgtctcgt ctagtggata ttttgagtga
    46201 aaatctggat gaaggtttcc aatatttccc gtctgatcaa atcacagatc atccagaacg
    46261 tttcttagtt tcagaaatgg ttcgcgagaa agtcttgcac ctaactcgtg aagagattcc
    46321 gcattctgta gcagtagttg ttgactctat gaaacgagac gaagagacag acaaggttca
    46381 catccgtgca accatcatgg tcgagcgcga tagccaaaaa gggattatca tcggtaaagg
    46441 tggcgctatg cttaagaaaa tcggtagtat ggcccgtcgt gatatcgaac tcatgctagg
    46501 agacaaggtc ttcctagaaa cctgggtcaa ggtcaagaaa aactggcgcg ataaaaagct
    46561 agatttggct gactttggct ataatgaaag agaatactaa gtagaggtag gctcatgcct
    46621 gcttcttgtt tttacagaag gaggacttat gcctgaatta cctgaggttg aaaccgtttg
    46681 tcgtggctta gaaaaattga ttataggaaa gaagatttcg agtatagaaa ttcgctaccc
    46741 caagatgatt aagacggatt tggaagagtt tcaaagggaa ttgcctagtc agattatcga
    46801 gtcaatggga cgtcgtggaa aatatttgct tttttatctg acagacaagg tcttgatttc
    46861 ccatttgcgg atggagggca agtattttta ctatccagac caaggacctg aacgcaagca
    46921 tgcccatgtt ttctttcatt ttgaagatgg tggcacgctt gtttatgagg atgttcgcaa
    46981 gtttggaacc atggaactct tggtgcctga ccttttagac gcctacttta tttctaaaaa
    47041 attaggtcct gaaccaagcg aacaagactt tgatttacag gtctttcaat ctgcccttgc
    47101 caagtccaaa aagcctatca aatcccatct cctagaccag accttggtag ctggacttgg
    47161 caatatttat gtggatgagg ttctctggcg agctcaggtt catccagcta gatcttccca
    47221 gactttgaca gcagaagaag cgacagctat tcatgaccaa accattgctg ttttgagcca
    47281 ggctgttgaa aaaggcggtt ccaccattcg gacttatacc aatgcctttg gggaagatgg
    47341 aagcatgcag gactttcatc aggtctatga taagactggt caagagtgtg tacgctgtgg
    47401 taccatcatt gagaaaattc aactaggcgg acgaggcacc cacttttgtc caaactgtca
    47461 aaggagggac tgatgagaaa aatcatcgga atcactgggg gaattgcctc tggtaagtca
    47521 actgtgacaa attttctaag acagcaaggc tttcaagtag tggatgccga cgcagtcgtc
    47581 caccaactac agaaacctgg tggtcgtctg tttgaggctc tagtacagca ctttgagcaa
    47641 gaaatcattc ttgaaaacgg agaactcaat cgccctctcc tagctagtct catcttttca
    47701 aatcctgaag agcaaaaatg gtcgaagcaa attcaagggg agattatccg tgaggaactg
    47761 gctactttga gagaacagtt ggctcagaca gaagagattt tcttcatgga tattccccta
    47821 ctttttgagc aggactacag cgattggttt gctgagactt ggttggtcta tgtggaccga
    47881 gatgcccaag tagaacgctt aatgaaaagg gaccagttgt ccaaagatga agctgagtct
    47941 cgtctggcag cccagtggcc tttagaaaaa aagaaagatt tggccagcca ggttcttgat
    48001 aataatggca atcagaacca gcttcttaat caagtgcata tccttcttga gggaggtagg
    48061 caagatgaca gagattaact ggaaggataa tctgcgtatt gcctggtttg gtaattttct
    48121 gacaggagcc agtatttctt tggttgtacc ttttatgccc atcttcgtgg aaaatctagg
    48181 tgtagggagt cagcaagtcg ctttttatgc aggcttagca atttctgtct ctgctatttc
    48241 cgcggcgctc ttttctccta tttggggtat tcttgctgac aaatacggcc gaaaacccat
    48301 gatgattcgg gcaggtcttg ctatgactat cactatggga ggcttggcct ttgtcccaaa
    48361 tatctattgg ttaatctttc ttcgtttact aaacggtgta tttgcaggtt ttgttcctaa
    48421 tgcaacggca ctgatagcca gtcaggttcc aaaggagaaa tcaggctctg ccttaggtac
    48481 tttgtctaca ggcgtagttg caggtactct aactggtccc tttattggtg gctttatcgc
    48541 agaattattt ggcattcgta cagttttctt actggttggt agttttctat ttttagctgc
    48601 tattttgact atttgcttta tcaaggaaga ttttcaacca gtagccaagg aaaaggctat
    48661 tccaacaaag gaattattta cctcggttaa atatccctat cttttgctca atctcttttt
    48721 aaccagtttt gtcatacaat tttcagccca atcgatcggc cctattttgg ctctttatgt
    48781 acgcgactta gggcagacag agaatcttct ttttgtctct ggtttgattg tgtccagtat
    48841 gggcttttcc agcatgatga gtgcaggagt catgggcaag ctaggtgaca aggtgggcaa
    48901 tcatcgtctc ttggttgtcg cccagtttta ttcagtcatc atctatctcc tctgtgccaa
    48961 tgcctctagc ccccttcaac taggactcta tcgtttcctc tttggattgg gaaccggtgc
    49021 cttgattccc ggggttaatg ccctactcag caaaatgact cccaaagccg gcatttcgag
    49081 ggtctttgcc ttcaatcagg tattctttta tctgggaggt gttgttggtc ccatggcagg
    49141 ttctgcagta gcaggtcaat ttggctacca tgctgtcttt tatgcgacga gcctttgtgt
    49201 tgcctttagt tgtctcttta acctgattca atttcgaaca ttattaaaag taaaggaaat
    49261 ctagtgcgag taaaaatcaa tctcaaatgc tcctcttgtg gcagtatcaa ttacctaacc
    49321 agtaaaaatt caaaaaccca tccagacaag attgaggttt taaagtattg tcccaaggaa
    49381 aaaaaagtaa ccctacatct tgaatctaag tagattttat ggtaaaataa tagggatttt
    49441 aaggagtttg atatgtataa cctattatta accattttat tagtattatc tgttgtgatt
    49501 gtgattgcaa ttttcatgca accaaccaaa aaccaatcca gcaatgtatt tgatgccagt
    49561 tcaggtgatt tgtttgaacg cagtaaagct cgcggttttg aagctgtaat gcagcgtttg
    49621 acagggattt tagtcttttt ctggctagcc attgccttag cattgacggt attatcaagt
    49681 agataagaaa ataatgggca ggactaggtc tttgcctctt tttattttta aaggatgttt
    49741 gagaaggttt tacagtaaaa gaaaattaaa aaatctagaa agaaaatatg aaagatagaa
    49801 taaaagaata tttacaagac aagggaaagg tgactgttaa tgatttggct caggctttgg
    49861 gaaaagacag ttccaaggat tttcgtgagt tgattaaaac cttgtcctta atggaaagaa
    49921 agcaccaaat tcgttttgaa gaagatggta gtctgacatt agaaattaag aaaaaacatg
    49981 agattaccct caaggggatt tttcatgccc ataaaaatgg ctttggcttt gttagtctgg
    50041 aaggcgagga ggacgacctt tttgtaggga aaaatgatgt caactatgct attgatggtg
    50101 ataccgtcga ggtagtgatt aagaaagtcg ctgaccgcaa taagggaaca gcagcagaag
    50161 ccaaaattat tgatatccta gaacacagtt tgacaacagt tgtcgggcaa atcgttctgg
    50221 atcaggaaaa acctaagtat gctggctata ttcgttcaaa aaatcagaaa atcagtcaac
    50281 cgatttatgt taagaaacca gccctaaaat tagaaggaac agaagttctc aaggtcttta
    50341 tcgataaata cccaagcaag aaacatgatt tctttgtcgc gagtgttctc gatgtggtgg
    50401 gacactcaac ggatgtcgga attgatgttc ttgaggtttt ggaatcaatg gacattgtat
    50461 ccgagtttcc agaagctgtt gttaaggaag cagaaagtgt gcctgatgct ccgtctcaaa
    50521 aggatatgga aggtcgtctg gatctaagag atgaaattac ctttaccatt gacggtgcgg
    50581 atgccaagga cttggacgat gcagtgcata tcaaggctct gaaaaatggc aatctggagc
    50641 ttggggttca catcgcagat gtttcttatt atgtgaccga ggggtctgcc cttgacaagg
    50701 aagcccttaa ccgtgcgact tctgtttacg tgacagaccg agtggtgcca atgcttccag
    50761 aacgactatc aaatggcatc tgctctctca atccccaagt tgaccgcctg acccagtctg
    50821 ctattatgga gattgataaa catggtcgtg tggtcaacta taccattaca caaacagtta
    50881 tcaagaccag ttttcgtatg acctatagcg atgtcaatga tatcctagct ggcgatgaag
    50941 aaaagagaaa agaatatcat aaaattgtac caagtatcga actcatggcc aagcttcatg
    51001 aaactttaga aaacatgcgt gtgaaacgtg gagctctcaa ttttgatacc aatgaagcga
    51061 agattttagt ggataaacaa ggtaagcctg ttgatatcgt tcttcggcag cgtggcattg
    51121 ccgagcggat gattgagtct tttatgttga tggctaatga aacagttgcc gaacatttca
    51181 gcaagttgga tttgcctttt atctatcgaa ttcacgagga gcctaaggct gaaaaggttc
    51241 agaagtttat tgattatgct tcgagttttg gcttgcgcat ttatggaact gccagtgaga
    51301 ttagtcagga ggcacttcaa gacatcatgc gtgctgttga gggagaacct tatgcagatg
    51361 tattgtccat gatgcttctt cgctctatgc agcaggctcg ttattcggag cacaatcacg
    51421 gccactatgg actagctgct gactattata ctcactttac cagtccaatt cgtcgttatc
    51481 cagaccttct tgttcaccgt atgattcggg attacggccg ttctaaggaa atagtagagc
    51541 attttgagca agtgattcca gagattgcga cccagtcttc caaccgtgaa cgtcgtgcca
    51601 tagaagctga gcgtgaagtc gaagccatga aaaaggctga gtatatggaa gaatacgtgg
    51661 gtgaagagta tgatgcagtt gtatcaagta ttgtcaaatt cggtctcttt gtcgaattgc
    51721 caaacacagt tgaaggcttg attcacatca ctaatctacc tgaattttat catttcaatg
    51781 agcgtgattt gactcttcgt ggagaaaaat caggtatcac tttccgagtg ggtcagcaga
    51841 tccgtatccg tgttgaaaga gcggataaaa tgactggaga gattgatttt tcattcgtac
    51901 ctagtgagtt tgatgtgatt gaaaaaggct tgaaacagtc tagtcgtagt ggcatagggc
    51961 gtggttccaa tcgtcgttcg gataagaagg aagacaagag aaaatcagga cgctcaaatg
    52021 ataagcgtaa gtattcacaa aaagacaaga agaaaaaagg aaagaaacct ttttacaagg
    52081 aagtagctaa gaaaggagcc aagcatggca aagggcgagg gaaaggtcgt cgcacaaaat
    52141 aaaaaggcac gccacgacta tacaatcgta gatacgctag aggcagggat ggtcctgact
    52201 ggaactgaaa tcaagagtgt acgagctgct cgaattaatc tcaaggatgg ctttgctcaa
    52261 gtgaaaaatg gagaagtttg gctgagcaat gttcatatcg cgccttacga agagggcaat
    52321 atctggaacc aggaaccaga acgtcgtcgt aaactcctgc tccataaaaa gcaaattcaa
    52381 aaattggaac aagagaccaa agggacagga atgaccttag ttccccttaa ggtctatata
    52441 aaagatggct acgctaagct tcttttagga cttgccaaag ggaagcatga ctatgacaaa
    52501 cgggagtcta tcaaacgtcg tgagcaaaat cgagatatcg cgcgtgtgat gaaagctgtt
    52561 aatcagcgat aaaaagagga attgaaaatg gaaaaattag ttgcctataa acgcatgcct
    52621 ttgtggaata aacaaacaat gcctgaagct gttcagcaaa agcacaatac aaaagttggg
    52681 acttggggga aaattactgt cttgaaggga gctctcaagt ttattgaatt gacagaagaa
    52741 ggggaagttc tagctgaaca cctctttgaa gcaggggcag acaatccaat ggcccaacct
    52801 caagcctggc accgagtgga agctgccaca gatgatgtgg aatggtactt ggaattttat
    52861 tgtaaacctg aggattattt tgctaaaaaa tacaatacca atcctgttca ttcagaggtc
    52921 ctagaggcca tgcagacagt gaaacaaggg aaagctttgg atttgggttg tggtcagggg
    52981 cgtaattctc tttttctagc ccagcaagat tttgatgtga cggctgtaga tcaaaatgga
    53041 ctagctcttg aaatcttgca aagcattgtg gagcaggaag atttggacat gcctgttggc
    53101 ctttacgata tcaattcagc tagcattgaa caagaatatg attttatcgt ttcaacagtt
    53161 gttctcatgt ttctacaagc ggaccgcatt ccagctatta ttcaaaatat gcaggagaaa
    53221 accagtgttg gtggttacaa ccttatcgtt tgtgccatgg acacggagga ttatccttgc
    53281 tcggttaact tcccattcac ctttaaagaa ggagaactgg cagactatta caaggattgg
    53341 gaattggtta agtacaatga aaatccaggc catttgcacc gtcgcgatga gaatggcaat
    53401 cgtattcaac tacgctttgc gaccttacta gctaagaaaa tcaagtaaac atacatgaag
    53461 attaggaatt ttcctgatct tttttctttt ttacgaatga tatagaaaag gagggaattc
    53521 atgtttgttg cgagagatgc taggggagaa ttggtaaatg tgttagagga taaacttgag
    53581 aagcaagcat acacctgccc agcttgtgga ggccagatcc atttgcgtca aggaccaagt
    53641 gtacggacgc attttgccca taaatcctta aaagactgtg attttttctt tgaaaatgaa
    53701 agtccagaac acctggccaa taaggaatcc ctctatcact ggttgaaaaa agagacaaag
    53761 gttcaattag agtacccgct ttcagaactt aaacagattg cggatgtatt tgtaaatggc
    53821 aatctagctc tagaagttca gtgtagtccc ttgcctcaga aagtccttaa agagcgaagt
    53881 gagggctatc gtagtcaggg ttaccaagta ctgtggttgc tgggtcaaaa actgtggctc
    53941 aaggagcgtt tgactcgtct acagcaaggt tttctttatt tcagtcaaaa catgggcttt
    54001 tatgtttggg aattagacaa ggaaaaacaa gttttaagac tcaaatacct gatttaccag
    54061 gatctccgcg gtaaactcca ttatcaaatc aaggaatttt cctatggtca aggtagttta
    54121 ttggaaatat tgcgtcttcc ctataagaga caaaaaatat ctcattttac agtttctgag
    54181 gacaaggaca tctgtcgcta tatccggcaa caactttatt atcaaaatct cttttggatg
    54241 aaagaacaag cagaagccta tcaaaaggga gaaaatatcc tgacttatgg actgaaagaa
    54301 tggtatccac aaattcgacc aatagtgggc aaatttttcc agattgaaca agacttgact
    54361 agctattatc agcactttta tacctattac caaaaaaatc ctcaaaatga ttggcaaaag
    54421 ctttatccac cagcctttta tcagcaatat ttcttgaaaa atatggtaga atagaaagga
    54481 tggaggaatc taatggtatt acaaagaaat gaaataaatg aaaaagatac atgggatcta
    54541 tcaacgatct acccaactga ccaggcttgg gaagaagcct taaaagattt aacagaacaa
    54601 ttggagacag tagcccagta tgaaggccat ctcttggata gtgcggataa cctactagaa
    54661 atcactgaat tttctcttga aatggaacgc cagatagaga agctttacgt ttatgctcat
    54721 atgaagaatg accaggatac acgtgaagct aagtatcaag agtactatgc caaggccatg
    54781 acactctaca gccagttaga ccaagccttt tcattctatg agcctgaatt tatggagatt
    54841 agcgaaaagc agtatgctga ctttttagaa gctcaaccaa agctgcaggt ttatcaacac
    54901 tattttgaca agctcttgca aggcaaggat cacgttcttt cacaacgtga agaagaatta
    54961 ttggctggag ctggagaaat ctttggttca gcaagtgaaa ccttcgctat cttggacaat
    55021 gcggatattg tgttccctta tgtcctagac gatgatggta aagaagttca gctatctcat
    55081 gggacttaca cacgtttgat ggagtctaaa aaacgtgagg ttcgccgtgg tgcctatcaa
    55141 gctctttatg cgacttacga acaattccaa cacacctatg ccaaaacctt gcaaaccaat
    55201 gttaaggtgc aaaattaccg tgctaaagtt cgtaactaca agagtgctcg tcatgcagcc
    55261 ctcgcagcga attttgttcc agaaagtgtt tatgacaatt tggtagcagc agttcgcaag
    55321 catttgccac tcttacatcg ctatcttgag cttcgttcaa aaatcttggg gatttcagat
    55381 ctcaagatgt acgatgtcta cacaccgctt tcatctgttg aatacagttt tacctaccaa
    55441 gaagccttga aaaaagcaga agatgctttg gcagtcttgg gtgaggatta cttgagccgt
    55501 gttaaacgtg ccttcagcga gcgttggatt gatgtttacg aaaatcaagg caagcgttca
    55561 ggtgcctact ctggtggttc ttacgatacc aatgccttta tgcttctcaa ctggcaggac
    55621 aatctggaca atctctttac tcttgttcat gaaacaggtc acagtatgca ttcaagctat
    55681 actcgtgaaa ctcagcctta tgtttacggg gattactcta tctttttggc tgagattgcc
    55741 tcaactacca atgaaaatat cttgacggag aaattattgg aagaagtgga agacgacgca
    55801 acacgctttg ctattctcaa taacttccta gatggtttcc gtggaacagt tttccgccaa
    55861 actcaatttg ctgagtttga acacgccatt caccaagcag atcaaaatgg ggaggtcttg
    55921 acaagcgatt tcctaaataa actctacgca gacttgaacc aagagtatta tggtttgagt
    55981 aaggaagaca atcctgaaat ccaatacgag tgggctcgca ttccacactt ctactataac
    56041 tactatgtat atcaatattc aactggcttt gcggccgcct cagccttggc tgaaaaaatt
    56101 gtccatggta gtcaagaaga ccgtgaccgc tatatcgact acctcaaggc aggtaagtcg
    56161 gactatccac ttaatgtcat gagaaaagct ggtgttgata tggagaagga agactacctc
    56221 aacgatgcct ttgcagtctt tgaacgccgt ttaaatgagt ttgaagccct tgttgaaaaa
    56281 ttaggattgg cataaaatgg ttgaatcgta tagtaagaat gctaaccata acatgcgtcg
    56341 tcctgtcgtc aaagaagaaa ttgtagactt gatgcgtcag cgtcaaaagc aggtcacagg
    56401 tttcttgaaa gaattggaag actttgcccg caaggaaaat attcctatta ttccccatga
    56461 aacggttgct tatttccgtt ttcttatgga aaccatgcag cctaaaaata ttctggaaat
    56521 tgggacggct atcggttttt cagctctctt gatggctgaa catgcgccaa atgctaagat
    56581 tacaactatt gatcgtaatc cagaaatgat tggttttgcc aaggaaaatt ttgcccagtt
    56641 tgacagtcgc aagcaaatca ctctcctaga gggagatgcg gtggatgtct tatctacact
    56701 gacagagtct tatgatttcg tatttatgga ttctgccaag tctaaataca tcgtctttct
    56761 gccagaaatc ctcaaacatt tggaagttgg tggtgtggtt gtcttggatg atatttttca
    56821 aggtggtgat gttgccaagg atattatgga agtccgtcgt ggtcagcgaa ccatttatcg
    56881 aggccttcaa aaattatttg atgcaacctt agacaatcca gaactcaccg caacattagt
    56941 gcctttagga gatggtattc tcatgcttcg taaaaatgta gcagatgttc aactgtctga
    57001 aagcgaatga ttttcagaaa aatttaagaa aaaatagtaa aatagataga gtaacactta
    57061 tctcaaagga gtagacatga agaaaaaatt attggcaggt gccatcacac tattatcagt
    57121 agcaacttta gcagcttgtt cgaaagggtc agaaggagca gaccttatca gcatgaaagg
    57181 ggatgtcatc acagaacatc aattttatga gcaagtgaaa aacaaccctt cagcccaaca
    57241 agtcttgtta aatatgacca tccaaaaagt ttttgaaaaa caatatggct cagagcttga
    57301 tgataaagag gttgatgata ctattgccga agaaaaaaaa caatatggcg aaaactacca
    57361 acgtgtcttg tcacaagcag gtatgactct tgaaacacgt aaagctcaaa ttcgtacaag
    57421 taaattagtt gagttggcag ttaagaaggt agcagaagct gaattgacag atgaagccta
    57481 taagaaagcc tttgatgagt acactccaga tgtaacggct caaatcatcc gtcttaataa
    57541 tgaagataag gccaaagaag ttctcgaaaa agccaaggca gaaggtgctg attttgctca
    57601 attagccaaa gataattcaa ctgatgaaaa aacaaaagaa aatggtggag aaattacctt
    57661 tgattctgct tcaacagaag tacctgagca agtcaaaaaa gccgctttcg ctttagatgt
    57721 ggatggtgtt tctgatgtga ttacagcaac tggcacacaa gcctacagta gccaatatta
    57781 cattgtaaaa ctcactaaga aaacagaaaa atcatctaat attgatgact acaaagaaaa
    57841 attaaaaact gttatcttga ctcaaaaaca aaatgattca acatttgttc aaagcattat
    57901 cggaaaagaa ttgcaagcag ccaatatcaa ggttaaggac caagccttcc aaaatatctt
    57961 tacccaatat atcggtggtg gagattcaag ctcaagcagt agtacatcaa acgaatagtc
    58021 caaatcaatg agtcagggaa aaaactctac ttcaggaaaa aatgaagcaa acattcccac
    58081 aataaaacgc gtagtacaag gtttgtactg acccccaaaa gttagacaaa taatttatcc
    58141 gaaggattta gttctgtatt gcacagggct aagtcctttt agttttacct taattctctt
    58201 gttgttgtaa taatcaatat agtctataat ggcttgttcc aattgattaa gtgatttaaa
    58261 tgttttctca tagccataaa acatttcgga ttttaaaata ccaaagaaag attccatcct
    58321 accgttgtct tggctgtttc ccttgcgtga catagatgct tgaattccct tactctctag
    58381 gaaccgatga taagaatcgt gttggtattg ccagtcttgg tcactatgga gaatcgtatt
    58441 ctcgtagtgc ttctctttga atgcctgttc caacattaac gatcaatcaa tttaatcatg
    58501 tacctaatat
//
LOCUS       DAVKLW010000004        54619 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKLW010000004 DAVKLW010000000
VERSION     DAVKLW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.004"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            165..479
                     /locus_tag="VW324_000215"
     CDS             165..479
                     /locus_tag="VW324_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725917.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            489..695
                     /locus_tag="VW324_000216"
     CDS             489..695
                     /locus_tag="VW324_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9725918.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            695..892
                     /locus_tag="VW324_000217"
     CDS             695..892
                     /locus_tag="VW324_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725919.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            895..1629
                     /locus_tag="VW324_000218"
     CDS             895..1629
                     /locus_tag="VW324_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9725920.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1641..2282
                     /locus_tag="VW324_000219"
     CDS             1641..2282
                     /locus_tag="VW324_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9725921.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            2300..2530
                     /locus_tag="VW324_000220"
     CDS             2300..2530
                     /locus_tag="VW324_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725922.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2566..2646
                     /locus_tag="VW324_000221"
                     /pseudo
     CDS             2566..2646
                     /locus_tag="VW324_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2927..4690
                     /gene="aspS"
                     /locus_tag="VW324_000222"
     CDS             2927..4690
                     /gene="aspS"
                     /locus_tag="VW324_000222"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9725923.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            4668..5609
                     /locus_tag="VW324_000223"
     CDS             4668..5609
                     /locus_tag="VW324_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9725924.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5730..5810)
                     /locus_tag="VW324_000224"
                     /pseudo
     CDS             complement(5730..5810)
                     /locus_tag="VW324_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5946..6932)
                     /locus_tag="VW324_000225"
     CDS             complement(5946..6932)
                     /locus_tag="VW324_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9725925.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(6942..7742)
                     /locus_tag="VW324_000226"
     CDS             complement(6942..7742)
                     /locus_tag="VW324_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9725926.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNIYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDSNYHK"
     gene            complement(7995..8837)
                     /locus_tag="VW324_000227"
     CDS             complement(7995..8837)
                     /locus_tag="VW324_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9725927.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8839..10131)
                     /locus_tag="VW324_000228"
     CDS             complement(8839..10131)
                     /locus_tag="VW324_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9725928.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(10242..11513)
                     /locus_tag="VW324_000229"
     CDS             complement(10242..11513)
                     /locus_tag="VW324_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9725929.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            12063..13580
                     /gene="malQ"
                     /locus_tag="VW324_000230"
     CDS             12063..13580
                     /gene="malQ"
                     /locus_tag="VW324_000230"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9725930.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            13606..15864
                     /gene="glgP"
                     /locus_tag="VW324_000231"
     CDS             13606..15864
                     /gene="glgP"
                     /locus_tag="VW324_000231"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9725931.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(16909..17481)
                     /locus_tag="VW324_000232"
     CDS             complement(16909..17481)
                     /locus_tag="VW324_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725932.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALEGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(18326..19174)
                     /locus_tag="VW324_000233"
     CDS             complement(18326..19174)
                     /locus_tag="VW324_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9725933.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSKLTEITIEAEI
                     LVGKAF"
     gene            19326..19604
                     /locus_tag="VW324_000234"
     CDS             19326..19604
                     /locus_tag="VW324_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725934.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            19606..21669
                     /locus_tag="VW324_000235"
     CDS             19606..21669
                     /locus_tag="VW324_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9725935.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRICVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(21747..23003)
                     /locus_tag="VW324_000236"
     CDS             complement(21747..23003)
                     /locus_tag="VW324_000236"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9725936.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            23145..25610
                     /locus_tag="VW324_000237"
     CDS             23145..25610
                     /locus_tag="VW324_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9725937.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKAGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            25675..26586
                     /locus_tag="VW324_000238"
     CDS             25675..26586
                     /locus_tag="VW324_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9725938.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            26816..27514
                     /gene="dapD"
                     /locus_tag="VW324_000239"
     CDS             26816..27514
                     /gene="dapD"
                     /locus_tag="VW324_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9725939.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            27582..28712
                     /locus_tag="VW324_000240"
     CDS             27582..28712
                     /locus_tag="VW324_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9725940.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            28724..29263
                     /locus_tag="VW324_000241"
     CDS             28724..29263
                     /locus_tag="VW324_000241"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9725941.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            29247..29924
                     /locus_tag="VW324_000242"
     CDS             29247..29924
                     /locus_tag="VW324_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9725942.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(29996..30895)
                     /gene="galU"
                     /locus_tag="VW324_000243"
     CDS             complement(29996..30895)
                     /gene="galU"
                     /locus_tag="VW324_000243"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9725943.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(30917..31933)
                     /locus_tag="VW324_000244"
     CDS             complement(30917..31933)
                     /locus_tag="VW324_000244"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9725944.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            32145..32584
                     /locus_tag="VW324_000245"
                     /pseudo
     CDS             32145..32584
                     /locus_tag="VW324_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(32699..33124)
                     /locus_tag="VW324_000246"
     CDS             complement(32699..33124)
                     /locus_tag="VW324_000246"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9725945.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(33192..33842)
                     /gene="phoU"
                     /locus_tag="VW324_000247"
     CDS             complement(33192..33842)
                     /gene="phoU"
                     /locus_tag="VW324_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9725946.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLSDLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(33857..34609)
                     /gene="pstB"
                     /locus_tag="VW324_000248"
     CDS             complement(33857..34609)
                     /gene="pstB"
                     /locus_tag="VW324_000248"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9725947.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(34611..35426)
                     /gene="pstA"
                     /locus_tag="VW324_000249"
     CDS             complement(34611..35426)
                     /gene="pstA"
                     /locus_tag="VW324_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9725948.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(35419..36282)
                     /gene="pstC"
                     /locus_tag="VW324_000250"
     CDS             complement(35419..36282)
                     /gene="pstC"
                     /locus_tag="VW324_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9725949.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(36400..37275)
                     /locus_tag="VW324_000251"
     CDS             complement(36400..37275)
                     /locus_tag="VW324_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU9725950.1"
                     /translation="MKFKKILTLAAIGLSGFGLVACGNQSATSKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(37442..38773)
                     /locus_tag="VW324_000252"
     CDS             complement(37442..38773)
                     /locus_tag="VW324_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9725951.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(38774..39481)
                     /locus_tag="VW324_000253"
     CDS             complement(38774..39481)
                     /locus_tag="VW324_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9725952.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGKQEGDDSDNIADNSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(39565..40050)
                     /locus_tag="VW324_000254"
     CDS             complement(39565..40050)
                     /locus_tag="VW324_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9725953.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            40036..40594
                     /locus_tag="VW324_000255"
                     /pseudo
     CDS             40036..40594
                     /locus_tag="VW324_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40909..42600)
                     /locus_tag="VW324_000256"
     CDS             complement(40909..42600)
                     /locus_tag="VW324_000256"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9725954.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            42736..43182
                     /gene="argR"
                     /locus_tag="VW324_000257"
     CDS             42736..43182
                     /gene="argR"
                     /locus_tag="VW324_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9725955.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            43233..45767
                     /gene="mutS"
                     /locus_tag="VW324_000258"
     CDS             43233..45767
                     /gene="mutS"
                     /locus_tag="VW324_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9725956.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            45914..47608
                     /gene="patA"
                     /locus_tag="VW324_000259"
     CDS             45914..47608
                     /gene="patA"
                     /locus_tag="VW324_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9725957.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(47938..48201)
                     /locus_tag="VW324_000260"
                     /pseudo
     CDS             complement(47938..48201)
                     /locus_tag="VW324_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            48399..50165
                     /gene="patB"
                     /locus_tag="VW324_000261"
     CDS             48399..50165
                     /gene="patB"
                     /locus_tag="VW324_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9725958.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            50304..50993
                     /locus_tag="VW324_000262"
     CDS             50304..50993
                     /locus_tag="VW324_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9725959.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            50997..51176
                     /locus_tag="VW324_000263"
     CDS             50997..51176
                     /locus_tag="VW324_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9725960.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            51163..52512
                     /locus_tag="VW324_000264"
     CDS             51163..52512
                     /locus_tag="VW324_000264"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9725961.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVIIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            52636..54096
                     /locus_tag="VW324_000265"
     CDS             52636..54096
                     /locus_tag="VW324_000265"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9725962.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            54110..54544
                     /locus_tag="VW324_000266"
     CDS             54110..54544
                     /locus_tag="VW324_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9725963.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVSELIPYWWGEERASKFNER"
BASE COUNT        16038 a        10305 c        11890 g        16386 t
ORIGIN      
        1 gaagagtatt aatgatagat aaagtggtca ggaacttact cctgaccttt ttcttttgca
       61 aaatgacaaa aatcataaac tttttgacaa atatacttgt caaaaagaaa aagatatgtt
      121 acaatgaatt caagttaaga aataggaagc agaaaggagt tataatgtgg gtactaggat
      181 ttatactatt tatgattttc ttttattcta ataattctaa aaaaatcaag aaactagaga
      241 ataaaatcaa aaaacttgag cgaaaagaga aaggaaacgc agaaatgtcg agattattac
      301 aagaaatgat tggaaaggaa ccaattataa cgggagtgta tattgggcca gataactggg
      361 aagttgtgga tgttgatgag gaatgggtca aactacgtag tgttgataat acgggaaaag
      421 aaaaattcaa gttgcaacgt attgaagata tccaaaccgt tgaatttgac ggagagtagg
      481 tgtgtaacgt gcaactaaaa aatcgtctaa aagagcttcg agctcgcgat ggtctcaatc
      541 aaaccgacct agccaaactg gcaggggttt ccagacagac cattagccta ctagaacggg
      601 atgagtacac cccatccatt atcattgccc tgaaaatatc tcaaattttc aacgaaacag
      661 tcgaatcggt atttcgtttg gaggaggacg agtgatgaac aagtataaag tgatttatta
      721 tgtagtggtc atagctttat tagtcagcgt atttctactg attgggatgg acctaagctg
      781 gtttaatccc tatcaaagcg accaatttgt ttgggtctac tttgctctca tcccagtaat
      841 tgaatggatt gaaaagaaat ccaaaaatct agcaagtgaa aaaggagaat gaatatgaaa
      901 aagaaacgta gattgttatt tttgatgtct attgtcttgg gggggttctt gggcatgttt
      961 gtagggatgt ttaaggcacg tgtcgaatcc cacgaaatta ttttagatgt aaaagccttg
     1021 atgccatgga tatcagctat ttgtttactg ataggtttca ttagtatgtt tttgactttc
     1081 aatttcttaa agaaaagcag aaaatttcat tccttgtatc aagaggaaat ggatgacgat
     1141 ctgaatgaaa cctattatgt gcaaatgtat cggaatcttg agtttggaac cattgctttt
     1201 aatattacag gtgtagcgat tccattggct atttttattt cattaagtga ggtgattata
     1261 ttgcatacaa accctcaaac atttttcctt tctttcttac tctttgtggt attcttagtc
     1321 gctcaaaaat ctctttttaa aaccattgcg attgttcgtc agtttgattt ggaatttttc
     1381 gctacaccaa aggatgtctt gaactatata aattcttatg atgaagggga gcgtcaggct
     1441 aatttggaac agagttttcg aattttattc caattacacc aatatgtctt accagcctta
     1501 tatatttttc ttattatcat ttctttcttg acaggagaga ttcagttact agctttcttg
     1561 cttgtaggag ccatccatgt ttatatcaat gtgatgcagt tacctatggt aaaacgttat
     1621 ttcaaataaa ggagttatct atgaaattac ttaaaaacct tggctggatt cttctagccc
     1681 ttctatcctt tttctttatc tatggcttta ttcaggggct cgcgactgcg tcgcttgctt
     1741 taggcgcttc accctatgct gttactctac tctatgtggt cttggctgga gtatatgtgt
     1801 acggcattta caaatggtat cagaaagctc ctgtttatat tgagaagagc ggctttaacc
     1861 gatttatttg gcttcctgcc ttggtttggt tcttatctct ggtcgttcaa ttcttcttgc
     1921 cagatgatcc ttcagtaaat cagcaaatag cgacagactt gaccttgtct caaccacttt
     1981 tctcattctt tgctgtggtt atttttgctc ctttgacgga agagattgtt tttagaggga
     2041 tgttagcacg ctatctcttt cctaagcagg acaatagtaa acgaaccctg atttttcttc
     2101 tggtatctag tcttctattt gccttgattc attttccagg tgatgtgcaa caattttttg
     2161 tctattttag ccttggtttt agtttgggtt tggcttacgt tagcagaaaa ggtctggtct
     2221 acagtatttc tcttcacgct ttgaataatt tagtcggctt tttgatgatt ctcatgctat
     2281 aatagagtca ggaggtcaca tgaaacgagt aattttatta gcagtgattc aggcagtcgt
     2341 tctatttttc atcattggag cgctagctta tgccttcaaa ggcgatttct tctataacta
     2401 tctagcagtt gtctttgctc ctattgcagg tgtactgcgt tttgggacgg cttacataac
     2461 ggaaattgtc ttgcctcgaa aggcagccga aatcgctgaa aagcgtaaag caggcaaaaa
     2521 ttcaaaataa aagagtccgg tgaagttcat cggatttttg tattatactc aatgaaaatc
     2581 aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac actgttttga ggttgtggat
     2641 agaactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc
     2701 agctcaaaac atggttttga ggttgtagat gaaactgacg aagtcagtaa ccatacctat
     2761 ggtaaggcga cgctgacgtg gtttgaagag attttcgaag agtattagcg ctaattttta
     2821 gggctttcac ctgactttta aagactggca aacttttctg atgattttca tgaagagcct
     2881 gcgcttttat ggtaaaatag taacagaata aaagaggaga gaaataatga aacgtagtat
     2941 gtatgctggt cgtgttcgtg aggaacacat cggacaagaa ataaccttga aaggatgggt
     3001 tggccgtcgt cgtgaccttg gtggtttgat ctttatcgat cttcgtgacc gtgaaggaat
     3061 catgcagttg gttatcaacc ctgaaaaagt atctgcagag gttatggcaa cagctgaaag
     3121 ccttcgtagc gaatttgtta ttgaggtgac tggtcaggtc gctgcgcgtg agcaagccaa
     3181 tgataagttg ccaactggtg cggttgagtt aaacgtgaca gctcttattg tgcttaacac
     3241 agctaagaca acaccatttg agattaagga tggcattgag gcaaatgacg atacacgttt
     3301 gcgttaccgt taccttgacc ttcgtcgtcc agaaatgttg gaaaatctta aacttcgtgc
     3361 caaggtgacc cactctatcc gcaactattt ggatgagttg gagtttatcg acgtggagac
     3421 accattcctt tctaagtcaa cgcctgaagg ggcgcgtgat tatttagtac cgtctcgtgt
     3481 taataagggg catttttacg ctcttcctca aagtccacaa atcacgaaac agctcttgat
     3541 gaatgctggt tttgaccgtt actaccaaat cgttaaatgt ttccgtgacg aggacttgcg
     3601 tggagaccgc cagcctgaat ttactcaggt cgacttggaa acgtccttcc ttactgagca
     3661 agaaatccaa gatattacag aaggcttgat cgcgcgcgtg atgaaggaaa caaaaggcat
     3721 cgaagtaacg ctaccgttcc ctcgtatgaa atacgatgat gctatggctc tttacggttc
     3781 tgacaagcca gatactcgtt ttgacatgtt gcttcaggac ttgacagaag tggtcaaagg
     3841 tgtagacttt aaagtctttt cagaagcacc tgctgtaaaa gcgattgtgg tcaaaggagc
     3901 tgcggacaac tattcacgta aagacatcga caagatgacg gaagtagcca aacagtatgg
     3961 tgccaaaggt cttgcttggg tcaaggtggt tgatggagaa ttaaacggac cagttgccaa
     4021 gttcttgact ggtatccaag aagaattgac aacagcgctt gctcttgaag ataaggactt
     4081 ggttctcttt gtggcggata cgcttgaagt ggctaatgca acactgggtg cccttcgtgg
     4141 acgtattgct aaagagcttg gcttgattga taatgataag ttcaacttcc tttgggtggt
     4201 tgactggccg atgtttgaat ggtctgaaga agaaggccgc tacatgagcg cccaccatcc
     4261 tttcaccctt ccacaggaag agactgctca cgaattagaa ggtgatttgg ctaaggttcg
     4321 tgccattgct tacgatatcg tcttgaacgg ttatgagctt ggtggtggta gccttcgtat
     4381 caaccaaaaa gaccttcaag aacgcatgtt caaggctctt ggtttctcaa ctgaagaagc
     4441 caatgaccag tttggtttcc ttcttgaagc catggactat ggtttcccac cacacggtgg
     4501 tttggctatc gggcttgacc gttttgtcat gttgcttgct ggagaagaaa atatccgtga
     4561 agtcattgcc tttcctaaga acaacaaggc aactgaccca atgacacaag ctccatcaac
     4621 agtcgctctc aaacaactag aggaactcag cttacaagta gaagaagatg aaacaaacaa
     4681 aacgaattaa gcggtggcgc tattatctgc gccgctttgc ttatcagata aaaattttac
     4741 gtgtcttaca aagtatctct cgagaaaagt atgatgagaa gatttcggcc tctctggtct
     4801 atggtttttt atcagcagta gcagttaatt tctttttcca accagggcat gtgtattcga
     4861 gtggtgcaac aggtctggca cagattatct ctgccttgag taatcactgg tttggttttc
     4921 atattccgat ttcgctaagc ttctacgcca ttaacttccc tttgatggtc ttagcttggt
     4981 atcagattgg ccataagttc accgtcttta cctttatcac ggtatctatg agttccttct
     5041 ttatccagtt tgtccctgtg gcaaccttga cggaggatcc cattatcaat tccctttttg
     5101 ggggtgttgt tatgggtttg gggattggtt ttgctcttcg aaacaatatc tccagtggtg
     5161 ggacggatat cgtcagcctg actattcgca agaaaacggg taagaatgtc ggtagtattt
     5221 ctttcttggt aaatggaact atcatgctga tagcaggttt aacctttggt tggaaatacg
     5281 ctctttattc tatgattacc atctttgtct ctagccgtgt gacagacgca gtctttacta
     5341 agcaaaagcg tatgcaggcc atgattgtga caaatcatcc agagaaggta attgaaaaaa
     5401 tccataaaaa attgcaccgc ggagcaacca tgatccacga tgcagaagga acctataatc
     5461 acgagagaaa ggcagtttta atcactgtca ttacacgtgc agagtttaat gaatttaaac
     5521 agattatgac acaagtggat ccaagctcct ttgtctctgt ctcggaaaat gttcatattc
     5581 taggaagatt tgttgagata gataattagt gattaatact cttcgaaaat ctcttcaaac
     5641 cacgtcagct tcaccttgcc gtaggtatgg ttactgactt cgtcagtttc atctacaacc
     5701 tcaaaaccat gttttgagct gacttcgtca gtttcatcta caacctcaaa acagtgtttt
     5761 gagcaacctg cggctagctt cctagtttgc actttgattt tcattgagta taaaaagatc
     5821 ttgggaaaag actcaatttc agtagaaaag gaagtaaatc ttcccacatt aaaacgcata
     5881 gtatcaagtt ttttcaatac ctgatactat gcgtttttgc ttttaacgac tttttgccca
     5941 gtctcttatt tttcgataat tttgtgggcg atcaattgac ggtaacaaat ttgtttattg
     6001 tttttattat cattaataat ctggagaata gtttcaaggg aaacacgacc aagctctaaa
     6061 ctattgatat cgacataggc tgccaagttg agcttgggat taaccgagtc aaagctgaga
     6121 acagggacat ccagctggtg tttggcaata tagttacaaa caccttcagc taggaggcta
     6181 tcggttgtga tgatagcatc aatttgtgga tcgtgcttga ataatcgctt gctaaattta
     6241 tagccctttt cttccagaaa ctcgtcggca aagtagatgc gattgttgtc agtggtaagt
     6301 ttgtaatgtt taagcgcctg ttcatagcct gttaaacggt ctttggtcac gaagagcttt
     6361 ttacttcctc cgataaaggc aatgcgtttg cagccttttt tgatgaaata ttcagtcgca
     6421 tcaaaaccag cttgaacatt gtcgttgtcg acaagtggga tgaaaggaga tagagattta
     6481 cctaagataa ggaaggggaa ctgttcttct gcgacgagtt ttacgagagg gtcttcttct
     6541 tgggcataga gaaagattag cccatctaca cgcttgccgt agaccatttg tgaaatagcg
     6601 ttgagacgct ccttctcatc tttccctgtt gctatctgaa tggcatagtg gttttcagat
     6661 gcgacttgag agatgccacg tagaaccgat ggaaagaaag gattctggta gaaggcgtct
     6721 gagtcatcag gaagaactaa tccgataacc tgagtatagc tgcttaccaa gctacgagcg
     6781 ttgaggtttg ggtggtagtt gagttccttc atagctttgc gaacgcgttt ttttgtttcg
     6841 tcgctaatgg ttgatttatt ttgaataaca cgggttacgg ttgaaggcga aacaccagca
     6901 gccttggcca cgtctttaat cgtaacgggc ataaaaatct cctatttatg gtaatttgaa
     6961 tcacggaaat gtgttttata aaagatagtg accagataca gaacaaaaag aatattttgt
     7021 acagtaatca gggttgtcat attttggcca aataatccca aaataagtgt aatcagagtc
     7081 ggcaatccta aacagttcaa gataaagtga tagcactctt taaaggttct aaatgaaaag
     7141 aggcgtgatt tcttagtgat ataaaggaga agactagctc ctagagagac gataaagaaa
     7201 ttcaaaccaa agaggaagct cgcaccgaga actaggaaga ggctgatata gacacgattt
     7261 tgttggtacc agtctttaga aattgcttgg gtcaagctgt ctttgctttt gaaactctca
     7321 gtctgaatgg ctcggtaaga gatgcgggtc agttccttgc tttccttgct gatgactagc
     7381 tcatttgtat cgaaatgcag ttgcaagtcc ttaggtaatt ccttgatttg acttggacca
     7441 atcacaacag aaggggcttg actagctgtt ccagtataag ttaatgtgcc atcgacaatt
     7501 gtagcatgtt cagagagatc ctggacaacc tcatttgtca gaggttcata gatattatcg
     7561 ataaaagttt ctagcggata ggtctcctgg gagctgtttt ggatagcaat gggtaccata
     7621 gataagctga taaggaagat actggtaaag agaagttgaa accagttgag ctcgaaacgt
     7681 ttggacaggg gcttacgaaa cccccaaata cttgaaaaat aggaaaatgg atatggaagc
     7741 ataggtatct ttctaaaagg tgttttctat atgagtatta ttatatagag aaatgcgctt
     7801 tatttcaagt ctaggagaaa aattgcttgc tacaaggata ttatttacag tgacaattgc
     7861 taataatcaa tgaaaatcaa agtgcaaact agccacaggt tgctcaaagc acaactttga
     7921 ggttggagat aaagctgacg tggtttgaag agattttcga agagtataaa atgaaaaagg
     7981 gtggcgggga taaattatcc cttgtcgcca ccacttgtaa gtcctgaaac aaagttcttt
     8041 tgtaggaaga agaagagaat acagattgga agggcgatga ggatagcacc tgctgagaag
     8101 taggcaatct tcaagttttt cgcattgtta acgaaggttt ggagacctac ggcaacagta
     8161 aagtattctt tctcacgaag caagaaacta gagaggatgt agtccccgaa aggtcccatg
     8221 aaggcccaga gagcttgtac ggcaaccatt gggcgaacaa gtggaagaac aatttgccag
     8281 aagcggcgga agtgtcctgc accgtctagt tttgcagatt cgtctaaaga cattggcact
     8341 gtatcgaagt agcctttcat gagccaagca ttcatcggga taccaccacc aacgtagagg
     8401 aagatgagga accagttgtg gttaagggcg ttcaacataa gcgccataac gaagaaggct
     8461 gtcaaagcgg ccatagttgg caccatttgg atgatcaaga agaagaccaa actttgttta
     8521 cgagccaaga agttgtaacg gctgtaggca taaccagcaa gtacgatgat acttgtttga
     8581 acagccatgg taattaaggc gataatcaaa gtgttgaggt accaagtacc gtacaaggtt
     8641 tcagtgaaga ggcctttaaa gttatcaaaa ttgaggtcga tattagtatc tagtttaaag
     8701 gctgagacgt tacctgcttt aaaggctgac ataatggtaa tcaacagtgg atagataatt
     8761 acaattgata gaccaatcag gtaaaggtaa gtaaggcttt gagtcagtct acgtttgagt
     8821 ttaattgagt tattcatctt agacgtcctc catatcaaat gcgtgtagtt tcttgaatgc
     8881 gatcatagag attgagatga caatgataga gataatcaag gtaacagctg ccgccattga
     8941 gtattgagga gatgtacctg ttgtcaaacg gtagatccat gagatcaaga tatcggttga
     9001 accagctcca cctccgacac taccaggtcc tccaccattg aagaggtaca tgatagagaa
     9061 gttgttaaag ttgaaggtgt attggctaat caaagtaggt gccgcaacag ccaaaatcat
     9121 tgggaaagtg atgttgcgga atttttgcca agcgttggca ccgtcaatat aagctgcttc
     9181 gtaaaggtcg ttaggaatag attgcaagat acccaaagtc aaaacgtaga tgtatgggaa
     9241 tccgagccaa ccttgcatca taatcaaggc aatcttagtc caagttgggt ctgttttcca
     9301 aggaataaga gctccatcaa ggaaaggaag gaatttagcc aagattggca atacttgagt
     9361 gttgatagca ccgacactat cgttaaacat gtttgagaat gtcaagatag tgatgaaggc
     9421 tgggacagcc caaggaagaa ggaaaataac accaaagata cgttttcctt tgataaatgg
     9481 ttggttggca atgatagctg tgaagatacc aattacgatt tgtaaagtag aagctgccaa
     9541 agcccaaatg atagtccaag aaagaacaga accaaaggca gaacggaagg tactcaagct
     9601 ccaaatgttt gtaaagttgg tcaaaccaac ccagtccaac aacttgtttg gtggcaagtg
     9661 ttggaagtcg tagttggtaa aggcgatcat caaggttacg ataactggga agataatcgc
     9721 gaaggtcatg gcaacataag atggaatgat caagaggtaa gggaagccat tttcataaat
     9781 ccctttgatc atgtctttga gtgtgcgtgg aactggaatt ccattgttaa tgcgttttgc
     9841 aatcgtatgt gcatctttga tatttgagaa ataaaagagt acataaacga ttacaaagat
     9901 tagatggaag gcaccacgaa tcagcataaa gagggaatta tcacgacctg gtttgtcacc
     9961 aagagtgatg aggttgctca attcaggggt tgcaagtgct aggaagtaaa ggacaaatac
    10021 gatggttaca ccaaggaaga taaaaccttt ggctttttgt ttattgtaaa tctgtcctaa
    10081 cccaggaatg atagacagca gggctgcttt actaggttgt tgcttttcca tattaactcc
    10141 tttcatagga tactactttt ttataagaaa gctaaattat ttgtgtctct attgataaat
    10201 tcaaaggggg atttgatttc cacccccctt gaacaaattt tttattcacc aaatttttgt
    10261 ttgattgttt ctttgatcaa tgttacagca tcgttagcag ctgttttagc atctttttga
    10321 ccacttacag catcaaagag catatttttc gctggatccc aaactgcaga catttgagag
    10381 atgtttggca gtggttgagt gttcttgaac tgtttgataa cagctgttgt caactcatcg
    10441 tttttacctt cagcgtatga acgagcctca gtattagctg ggatttcgtt agtcttatca
    10501 tataatactt tttgttgttc agttgcaaca aggaagtcta caaatttttg agaagcttca
    10561 aggttcttaa cggcttgagg aatgacccaa gctttaccac caccgaatgc agcatattct
    10621 tttccatttg gaagagttgg gatagttgca actccgtagt ttactttagc atctttaaag
    10681 gcttgagctt tccaaggtcc gtcgatgata gcagctgttt taccttcttg gaattgagtt
    10741 tggattaagt ttccagcacc ttctgtatct tgcatacctt taggccattt ttcgtaccaa
    10801 gatttagcgt agttgatacc tgcgatagaa ccgtcgtttg caagaccgat gtctttagcg
    10861 tctttaccgt tttggccaaa gacgtaagca ccgttaccgg caagaagtcc atatgtatag
    10921 tagaagtttg tccagtcagc taggaaggca gtagttttac catcttcacc agcgaatgcg
    10981 tatttgctat ctttagcaag gttttccaag tcagcaaatg tttttggagc atctttcacc
    11041 aagtctttgt tgtagtacat aacaagtgac tcgataacgg caggagcacc gtaaacttta
    11101 ccattagcag ctgttacaag agatttagtt gtgtcgtctg ttttagcacc atcgctcaat
    11161 ttcacttctg aaagttgtcc gtcagaacca aggctaccta cacggtcgta tggagccatc
    11221 ataacatcag ggacattacc agattggttg tcaagagaaa gtttatcaag acctcctaga
    11281 gcatcaccag ttttaagagt gacttttact ccagcttctt tttcataagc tttagcaacc
    11341 tcttcaatat agctcttata tccctcgtct acatatacag tgagttcttt gacttcagat
    11401 gaaccagaat cagcaggctt atcagcagtt ttgcttccgc aagctaccaa aagcaagcta
    11461 gcaagtgtag cagttccaag caccgcagtg ctcttcataa atttagatga catagtgtat
    11521 tcctcccaaa gaatagcaag ttttattgat aaggaaacgc aaacgttttc ctttatgagc
    11581 ttagtatagc acaaatagaa aacggttgca agtatttttt gtaaaaattt taaaaaattt
    11641 ttaagcttga tttgatagca taattttgca ttctaataga agaaaagtat gaaatagata
    11701 agaatacagt atttttaata aaaatatatg gaatctgtct aaagagggaa atcaggaggg
    11761 gctacctccc tgtttctaat ccctatctat tttctataga cgtttgtgct ttgttgcata
    11821 taaattcaag ctccctaagg aggttttgta ctttgtagac tgtctcgtga gagtcaaact
    11881 ttaggattag atagttttaa gttatctttg ctaaaatgag ttagtctcct taaaaataat
    11941 caaaaaagtt ttttaaaaac gcttgcaatt atgcttgaaa aggagtatac ttataagtaa
    12001 cgcaaacgtt tgcgtctgca aaatacgcaa cgttccatta ttttaacaca cgaggtgcta
    12061 ttatgaaaaa acgtcaaagt ggtgtgttga tgcacatctc ttctcttcca ggagcttacg
    12121 gaatcggatc atttggtcaa agtgcttacg acttcgttga tttcttggtc cgtacaaaac
    12181 aacgttactg gcaaatcctt ccattaggag caactagtta cggggattct ccttaccaat
    12241 ctttctcagc cttcgcagga aacactcatt ttatcgattt agatatcttg gtggagcaag
    12301 gtttgttgga agcaagtgac cttgaaggag ttgactttgg tagcgatgcg tctgaagttg
    12361 actatgctaa aatctactat gcacgtcgtc ctcttttaga aaaagcggtg aaacgtttct
    12421 ttgaagtcgg agatgttaaa gattttgaga aatttgctca agacaaccaa tcatggcttg
    12481 agctctttgc tgagtatatg gctatcaaag agtattttga caatcttgct tggactgaat
    12541 ggccagatgc agatgctcgt gctcgtaaag cttcagcact tgaaagctat cgtgagcaat
    12601 tggcagacaa gttggtttac caccgtgtga ctcaatactt cttcttccaa caatggttga
    12661 aattgaaagc ttacgctaac gacaaccaca tcgaaatcgt tggggacatg ccaatctacg
    12721 tagcggaaga ttcaagtgat atgtgggcaa atccacatct cttcaaaaca gatgtcaatg
    12781 gtaaggctac ttgtatcgca ggatgcccac cagatgagtt ttctgtaact ggtcagcttt
    12841 ggggtaaccc aatctatgac tgggaagcaa tggacaaaga cggctacaaa tggtggattg
    12901 aacgcttgcg tgaaagcttc aaaatctacg atatcgttcg tatcgaccac ttccgtggct
    12961 tcgaatctta ctgggaaatc cctgctggtt ccgatacagc agcacctggt gagtgggtga
    13021 aaggtccagg ctacaagctt tttgcagccg ttaaggaaga acttggtgag ctaaacatca
    13081 tcgcagaaga ccttggcttc atgacagatg aagtgatcga attgcgtgaa cgtactggct
    13141 tcccaggaat gaagattctt caatttgcct tcaacccaga agacgaaagc attgatagcc
    13201 cacacttggc acctgctaac tcagttatgt acacaggaac acacgataac aatacggttc
    13261 ttggttggta ccgtaacgag attgatgatg cgactcgtga gtacatggct cgttacacga
    13321 accgtaaaga atatgaaaca gtggtacacg ctatgcttcg tacagtattt tcatcagtta
    13381 gctttatggc aattgcaact atgcaagatt tactagaatt ggatgaggca gctcgtatga
    13441 acttcccatc tacccttggt ggaaactggt cttggcgtat gactgaagat caattgacac
    13501 cagctgtcga ggaaggtttg cttgacttga caacaattta tcgccgaatt aatgaaaatt
    13561 tggtagattt aaagaaataa gacaataatc aggagacaac taaacatgtt atcactacaa
    13621 gaatttgtac aaaatcgtta caataaaacc attgcagaat gtagcaatga agagctttac
    13681 cttgctcttc ttaactacag caagcttgca agcagccaaa aaccagtcaa cactggtaag
    13741 aaaaaagttt actacatctc agctgagttc ttgattggta aactcttgtc aaacaacttg
    13801 attaaccttg gtctttacga cgatgttaaa aaagaacttg cagctgcagg taaagactta
    13861 atcgaagttg aagaagttga attggaacca tctcttggta atggtggttt gggacgtttg
    13921 gctgcctgct ttatcgactc aattgctact cttggtttga atggtgacgg tgttggtctt
    13981 aactaccact ttggtctttt ccaacaagtt cttaaaaaca accaacaaga aacaattcca
    14041 aatgcatggt tgacagagca aaactggttg gttcgctcaa gccgtagcta ccaagtacca
    14101 tttgcagact ttactttgac atcaactctt tacgatattg atgttactgg ttatgaaaca
    14161 gcgactaaaa accgcttgcg tttgtttgac ttggattcag ttgattcttc tattattaaa
    14221 gatggtatca actttgacaa gacagatatc gctcgcaact taactctctt cctttaccca
    14281 gatgatagtg accgtcaagg tgaattgctc cgtatcttcc aacaatactt catggtttca
    14341 aacggtgcgc aattgatcat cgacgaagca atcgaaaaag gaagcaactt gcatgacctt
    14401 gctgactacg cagttgtcca aatcaacgat actcacccat caatggtgat tcctgaattg
    14461 attcgtcttt tgactgcacg tggtatcgag cttgacgaag caatctcaat tgttcgtagc
    14521 atgactgcct acactaacca cacaatcctt gctgaggcgc ttgaaaaatg gcctcttgaa
    14581 ttcttgcaag aagtggttcc tcacttggta ccaatcatcg aagaattgga ccgtcgtgtg
    14641 aaggcagagt acaaagatcc agctgttcaa atcatcgatg agagcggacg tgttcacatg
    14701 gctcacatgg atatccacta cggatacagt gttaacgggg ttgcagcact ccatactgaa
    14761 atcttgaaaa attctgagtt gaaagccttc tacgaccttt acccagaaaa gttcaacaac
    14821 aaaacaaacg gtatcacttt ccgtcgttgg cttatgcatg ctaacccaag attgtctcac
    14881 tacttggatg agattcttgg agatggttgg caccatgaag cagatgagct tgaaaaactt
    14941 ttgtcttatg aagacaaagc agctgtcaaa gaaaaattgg aaagcatcaa ggctcacaac
    15001 aaacgtaaat tggctcgtca cttgaaagaa caccaaggtg tggaaatcaa ttcaaattct
    15061 atctttgata tccaaatcaa acgtcttcac gagtacaaac gccaacaaat gaacgctttg
    15121 tacgtaatcc acaaatacct tgacatcaaa gctggtaaca tccctgctcg tccaatcaca
    15181 atcttctttg gtggtaaagc agctccagcc tacacaatcg ctcaagatat cattcacttg
    15241 atcctttgca tgtcagaagt tattgctaac gatccagcag tagctccaca cttgcaagta
    15301 gttatggttg aaaactacaa cgttactgca gcaagcttcc ttatcccagc atgtgatatc
    15361 tcagaacaaa tctcacttgc ttctaaagaa gcttcaggta ctggtaacat gaaattcatg
    15421 ttgaacggag ctttgacact tggtactatg gacggtgcta acgtggaaat cgctgagttg
    15481 gttggagaag aaaacatcta catcttcggt gaagattcag aaactgttat cgacctttac
    15541 gcaaaagcag cttacaaatc aagcgaattc tacgctcgtg aagctatcaa accattggtt
    15601 gacttcatcg ttagtgatgc agttcttgca gctggaaaca aagagcgctt ggaacgtctt
    15661 tacaatgaat tgatcaacaa agactggttc atgactcttc ttgatttgga agactacatc
    15721 aaagtcaaag agcaaatgct tgctgactac gaagaccgtg acgcatggtt ggataaagtc
    15781 atcgttaaca tttctaaagc aggattcttc tcatctgacc gtacaatcgc tcagtataac
    15841 gaagacatct ggcacttgaa ctaatactct tcgaaaatct cttcaaacca cgtcagcttt
    15901 atctgcaacc tcaaagcggt gctttgagca acctgcggct agcttcctag tttgctcttt
    15961 gattttcatt gagtataaga tacaaattta tactaataca tcttgtaaaa aagcgagttt
    16021 cgattgaaat tcgctttttt aaatgctgtg gatttgggtc aatcttgtct aaaaataggg
    16081 aaatcctaga tacagtgaag gctttaaatg ctggttttta ctgtcttcag ccttatattt
    16141 tttcgtagtt ggttacctca catctcttgt attcgcttac ataaagtatt ataatataat
    16201 tgtaggaaag aaggtgtttt tatgatatac acacttaaat tggtgttgtt tattaccttt
    16261 cttgtaataa gcttgttacc tgataagatt tttggaaaaa ataaaaaaat ttggaaaata
    16321 gtttttgcaa tattgacggc agtggcagca ttgtcattta tgtactaagt tattttaaga
    16381 atgtagggaa ataaacccta catttttttt agttttttct gttttctaaa ttctatttat
    16441 ccaagcgatt caacatttct tgcttcttcg cttcaagttc tgcacgcttt tcttcgattt
    16501 cggcatgttt tttctcgagt tcagaacaac ttgcaccatt tctaaattct tttcgccatc
    16561 aggagatagg gtgagtcgac atgtctatta ctcacccaaa gcagtcctac aaagcaggaa
    16621 ttttctgtta cttttttgga aatagtaacg tttatacagc tttgacactt cgtatcaaag
    16681 cgccaaacac actccgaggg atttacagaa agcagaaaag gaatgatctg gtataagatc
    16741 attccttttc tctctttttc tttaagtaat tatatacaat gtacgacgaa gtcgtcattg
    16801 caatgctgat ccaccaccta aagggaactt taaacaacat tgataagata aagaatataa
    16861 acaacgaaaa tacgttatac ccaattaatt ttattgtata tctcatgatt aaaagttaat
    16921 ccttccgttg ttaggaatgg catcattttt atcccataat tgtgctaaat aagtccctgg
    16981 tgataataaa ttcatagcga attctaaagc aacatcattt acaaaccaac tacctagata
    17041 tctagaaatt gctgaacgaa tagcactttt tgctgcatgt tttcctttta ctttaattag
    17101 atttgcaagg cctgcagtag ttccttctaa tgctaaagct attgcagtat ctaatagagc
    17161 acccatttga ttaactgtaa taccttgcca agctgctcta aatggagagt atgtaggtgg
    17221 gattgtataa tcgccttgta attgtcggtt aattacttct ttgatccatt gttgtgagac
    17281 gtctggatga aaagattgga tttcgtttgc aagtgtattg atttgttctt ctgttagaga
    17341 agtgacaggt tgaagttcca tatttgtttc aatttgcgat acttgttcag aagcgtatac
    17401 agctgaaaca cttggaatcg ctgatacaat taacacaatt gacgtcaaaa aaaccgaaat
    17461 aaatttcatt aatttgttca tgagcttttc tcctttttat ttgtatctgc ttacatttta
    17521 ttatatactg ttattatagt caagaaaaat atgctattat gttaaaaaaa tatttttcaa
    17581 aatataaatg gacggattta ttttggattt tatttgttat tttgacctgc ctctatattg
    17641 gtaaccatga tttgtttact ctcaatcatc aagaattctc ttttcgtggt agcgtttggg
    17701 gtctggtact ggccttatat cacttattgt tcattgataa gtttgtaatt tcgaatcgaa
    17761 aataaagatt agaactatga ttgactggct acttttaata ttgattttgg aggaagattt
    17821 tgtctctatt atttattatt ttaaatttat ctattttgta caagatctat tctttgagac
    17881 gggagaaatc gaaatacttg atttatacgg cctatatcat atttggggta aatgtagtat
    17941 atggtattca atggttatta agagaactga tttcaactat ttccccttaa tgtgtatgga
    18001 aactataaca aaacgcataa gattaaggtt tgtgtacctg ttcttatgcg tttttgtaat
    18061 attgaagatt ttcggtaagt tttatacctt tgggaagtct agtttatact caatgaaaat
    18121 caaagagtaa actagccgta ggttgctcaa agtacagctt tgaggttgca gataaaactg
    18181 acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa gtcagctcaa
    18241 aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacg tacggcaagg
    18301 cgacgttgac gtggtttgaa gagtattaga atgctttccc aaccaaaatc tctgcttcga
    18361 tggtaatctc tgtcagtttg ctccagtcaa ttttgctctg gtcaacgtga aagaggagag
    18421 gagtcatact gagtagggct tggagttgtt ctgctgtgat agtcttagtc agagaggcag
    18481 tttgactaga taggatggta aagtgttcct ggaaatgctc cttgatatct tggttagaat
    18541 actccttgtt tgtcagctgg tcctgtactc tttgacggat ttctttgagg tgattttcag
    18601 ttgggataac ctttatcaag ataccgtctt tggataaaac gcgacgaaat tctccatagt
    18661 tggcaggtga gaagatatca agcagaatat ccatgttagc gtcttttata ggaagtcgtg
    18721 ccaagtcgcc aacgaaccaa ttgactgccc agttgggttc acttttagca gcgatttgga
    18781 ctgaatcttt ggagatgtca aaggcataga aagttttttc agagtgactt tcttgtagtt
    18841 tgcgagaata gaatccttca ccacaaccga tatccaaaat tgttgtggta gtttttgagc
    18901 ttgcaagcaa gtcagataca gcatctaaga tagcttggta aaagccggct tctaggattt
    18961 gttgacggtt ttgaaaattt tccttgtcat agttagcaga ttgcttgatt tgaggtgcta
    19021 gattgacata gccaaatttc gccaagtcaa aagaatgacg gttgcagcac ttgaaattag
    19081 tctctaacag agtcagattt tcttgacaga taggacaggc aaaggcagtc gcagaagcaa
    19141 aacgctgaag tttgggcttg agatttgtat tcatagttta agtgtatcaa aagaggcaaa
    19201 tgaaagcaac tttaggaata agtagatggg agattcagta gaaataaaac taattctcca
    19261 atttatagaa tgaaattgct tttatatcta aattgctata taataatgat aaggaggaaa
    19321 taagtatgtt aaaagaagta ttaaccgttg caaaagttgc gaaaaaatct tcattatttt
    19381 tgggtggtgt cgcatttggt acccttggtt tgaaaatctt agcaagtaag gaagctaaaa
    19441 aaggttattc taaagctttg gctaaggctt acaagttgaa agacgagcta gatgcatctg
    19501 tttctgtcgt gaagcaacat ggagacgatg tcttgcaaga tgccaaatat ttgtacgagc
    19561 aagagaaaaa agaagagcaa ttagatagcc ttataggtga ataatatgtc ttttaaagtg
    19621 ctacatagag gataccaaca tatccgacta tcatcttctt tttcacttac cttggatatt
    19681 caagactatc ttcgttcctt ggcgagagat gaaaagggga ttgagtctat ccagttttac
    19741 atggatcaac agcactttac tctacgcata aaagaaggct tttctgtatt agataatgca
    19801 gaagcctttt taaaaagaat tgataaaggg aaagtttctg agttgatgac tcttcccatt
    19861 cgtagagaag agagtgctta ttctattgtt tcaggtgcag cggttaagcg tgtacttttt
    19921 cgtagttttg tgccgtatcc tattcgctat atatggactt gttatcaggc tttgggttat
    19981 attagagaag cctatcaaac actagcgcgt aaggaactaa cgatggaagt cttggactgt
    20041 tcggctattt tattgtcttt gtttatgaac caatccaaga cagctagcaa tatcatgttt
    20101 atgcttgatt tggggaatca tttagatcag tggtccttga aaaaaactgc aacagattta
    20161 gaacagagtc ttcttgcaaa agagagcgat gtattcctag tacagggcga tacagttgtt
    20221 agtatcaaga gttccgatgt tcaaatagga gatgtcttga tcttatctca aggaaatgaa
    20281 attctgtttg atggacaagt agtttcaggt ttaggtatgg tcaacgaaag ttccttgaca
    20341 ggagagagtt ttccagttga aaaaagagag tctgatttgg tttgtgcaaa tacagtatta
    20401 gaaactggag agttacgcat ttgtgtaaca gataatcaga tgaacagccg tattttacag
    20461 ctgattgagt tgatgaagaa atctgaagaa aacaagaaaa cgaaacaacg ctatttcatc
    20521 aagatggcgg ataaagtcgt caaatataat ttcttggggg ctgggctgac ttacctattg
    20581 acaggttctt tttctaaggc tatttctttc ctattggtcg atttctcctg cgctttgaaa
    20641 atctctactc ctgtagctta tttgacagtt atcaaggaag ggttgaaccg tgaaatggtg
    20701 attaaggatg gagatgttct ggagaaatat ctggaagttg atactttctt gtttgataag
    20761 acaggaacaa tcacaactag ttatcctata gttgaaaagg tgttaccttt tggagactat
    20821 agtgaggaag atattctcag aatcagtgcc tgtcttgagg aacacattta tcatcctatt
    20881 gctaatgcca tcgtcaagca agctgagata gagggaattg aacatgagga aatgcatggg
    20941 aaactccaat atatcgcaag caaggggatc aaatctcata tagatggcca accagttctt
    21001 attgggaatt atgtcttgat gcaggatgag cagattcata tcagttcaga acaaaatgct
    21061 ttaattgaag agtacaagag tcactacaat ctcttattct tggcttatca aaatgaattg
    21121 attggaatgt tctgcattca tactcctttg agaaaagaag caaaaacagc cttggataaa
    21181 cttaaggcac aggggaaaaa attgattctg gcaacaggag acaccttgat taggacagag
    21241 gaattagtca aagatttgcc ctttgatcag gtctatacag acttgaaacc tgatgggaaa
    21301 tttgagttag tagagaaact gcagaaagca ggtcacacta ttttgatggt tggagatgga
    21361 ttgaatgact cagcggcttt aactctatca gatatcggtg tggtgatgaa tgagagtgca
    21421 gatatttcta agcagatgag tgatatctta ttgttagata atcgtttgga tttcttccaa
    21481 gagttggatt cgttatcatc atctttgcaa acactcatca agaaaaatat tcaggatacc
    21541 gttgtcgtaa atagtagttt gattggcttt ggcttattta attggttcag tccttcaaat
    21601 ctctctatct tacataatct aacaaccttg cgcattgtcc tgcgtagcct gtctattaaa
    21661 aatagataga tgagagttat taacagcaaa aaggagttac ctttctcgag gttaactcct
    21721 ttgtttatag ataaatgttg aatagtttag taagtcaata caaagtattt tttcttccca
    21781 cgacggataa cagtcagttc attttctaac ttatcagcgt cactcaagac atagtcaagg
    21841 tcttggatgc ggtcgccgtt tacgtagatg gctccgtttt ggacgtcttc acgggcttgg
    21901 cgttttgagt taactatacc agatgagacg agcagttcca cgatattgtt gttttcgtct
    21961 gcctgtactt ggtagtttgg cacaccacga agtccttgtt tgagctcttt gacagaaagg
    22021 tttttgatgt ttcctgcaaa gagttgctca gtgatgttaa gtgcttcttt gtaggcttct
    22081 tctccgtgaa caagtgtaac aacttcacga gccaagactt tttgagccaa gcgttcgtgt
    22141 ggcgctgctt caaattgttt acgaatatct tcaatctcat caagtgacaa gaaagtaaag
    22201 attttcaaga agcgaacagc gtcagcgtcc atcacgttca tccagaattg gtacatttcg
    22261 tatggagaag tcttttcggg attgagccag acggcatttc cttctgattt accaaatttt
    22321 ttaccagttg catctgtgat tagtggaaca gtgataacgt gaccagtctt gtccgcctta
    22381 cgacgaagca attcggtacc agctgtcata tttccccact ggtcagaacc accgatttga
    22441 agagtgacat tatggtcttg gttaaggacg aagaagtcgt acccttgcat gatttggtaa
    22501 gcgaactcag tgtaagaaat tcctgtttcg atccgttttt taacagattc cttactcatc
    22561 atgtagttga ccgtgaagta ttttccaata tcacggagga agtcaatgaa gctgatgctg
    22621 ccaaaccagt cgtagttgtt gaccatgaca gccttgtttt cgccattttc aaagtcaaga
    22681 aaacgagaaa gttgtccttg gatagacttg acccagccat ctactgtgtc ttttgtttgg
    22741 agactacgtt cagcatcttt gaaggacgga tctccgatga gacctgtagc accgccaacg
    22801 agcgcataag gtttgtgacc tgctagttgc aagcgacgac ttgtcaagat tgcgacaagg
    22861 tggcctaggt gaaggctgtc agcagttgga tcgtagccag tataataaga aacttgacct
    22921 tcttctaggg ctttacgcaa agcttcttca tcagtcgttt gaaatatcaa accacgctct
    22981 tttagctcat caaaaatgtg catgtgtctt ttctcctttt taaaattatt gtttctacct
    23041 attgtatcac aaacttgggc aatagcctag cagaataggg aagaaagtgg ctattttatt
    23101 gaaaatttcc cttttgtggt ataatagata aagtgaggaa atccatgcaa aatcaattaa
    23161 atgaattaaa acgaaaaatg ctggaatttt tccagcaaaa acaaaaaaat aaaaaatcag
    23221 ctagacctgg caagaaaggt tcaagtacca aaaaatctaa aaccttagat aagtcagcca
    23281 ttttcccagc tattttactg agtataaaag ccttatttaa cttactcttt gtactcggtt
    23341 ttctaggagg aatgttggga gttgggattg ctttggggta cggagtggcc ttatttgaca
    23401 aggttcgggt gcctcagaca gaagaattgg tgaatcaggt caaggacatc tcttctattt
    23461 cagagattac ctattcggac gggacggtga ttgcttccat agagagtgat ttgttgcgca
    23521 cttctatctc atctgagcaa atttcggaaa atctgaagaa ggctatcatt gcgacagaag
    23581 atgaacactt taaagaacat aagggtgtag tacccaaggc ggtgattcgt gcgaccttgg
    23641 ggaaatttgt aggtttgggt tcctctagtg ggggttcaac cttgacccag caactaatta
    23701 aacagcaggt ggttggggat gcgccgacct tggctcgtaa ggcggcagag attgtggatg
    23761 ctcttgcctt ggaacgcgcc atgaataaag atgagatttt aacgacctat ctcaatgtgg
    23821 ctccctttgg ccgaaataat aagggacaga atattgcagg ggctcggcaa gcagctgagg
    23881 gaattttcgg tgtagatgcc agtcagttga ctgttcctca agcagcattt ttagcaggac
    23941 ttccacagag tcccattact tactctcctt atgaaaatac tggggagttg aagagtgatg
    24001 aagacctaga aattggctta agacgggcta aggcagttct ttacagtatg tatcgtacag
    24061 gtgcattaag caaagacgag tattctcagt acaaggatta tgaccttaaa caggactttt
    24121 taccatcggg cacggttaca ggaatttcac gagactattt atactttaca actttggcag
    24181 aagctcaaga acgtatgtat gactatctag ctcagagaga caatgtctcc gctaaggagt
    24241 tgaaaaatga ggcaactcag aagttttatc gagatttggc agccaaggaa attgaaaatg
    24301 gtggttataa gattactact accatagatc agaaaattca ttctgccatg caaagtgcgg
    24361 ttgctgatta tggctatctt ttagacgatg gaacaggtcg tgtagaagta gggaatgtct
    24421 tgatggacaa ccaaacaggt gctattctag gctttgtagg tggtcgtaat tatcaagaaa
    24481 atcaaaataa tcatgctttt gataccaaac gttcgccagc ttctactacc aagcccttgc
    24541 tggcctacgg tattgctatt gaccagggct tgatgggaag tgaaacgatt ctatctaact
    24601 atccaacaaa ctttgctaat ggcaatccga ttatgtatgc taatagcaag ggaacaggaa
    24661 tgatgacctt gggagaagct ctgaactatt catggaatat ccctgcttac tggacctatc
    24721 gtatgctccg tgaaaatggt gttgatgtca agggttatat ggaaaagatg ggttacgaga
    24781 ttcctgagta cggtattgag agcttgccaa tgggtggtgg tattgaagtc acagttgccc
    24841 agcataccaa tggctatcag accttagcta ataatggagt ttatcatcag aagcatgtga
    24901 tttcaaagat tgaagcagca gatggtagag tggtgtatga gtatcaggat aaaccggttc
    24961 aagtctattc aaaagctact gcgacgatta tgcagggatt gctacgagaa gttctatctt
    25021 ctcgtgtgac aacaaccttc aagtctaacc tgacttcttt aaatcctact ctggctaatg
    25081 cagattggat tgggaagact ggtacaacca accaagacga aaatatgtgg ctcatgcttt
    25141 cgacacctag attaacccta ggtggttgga ttgggcatga tgataatcat tcattgtcac
    25201 gtagagcagg ttattctaat aactctaatt acatggctca tctggtaaat gcgattcagc
    25261 aagcttcccc aagcatttgg gggaacgagc gctttgcttt agatcctagt gtagtgaaat
    25321 cggaagtctt gaaatcaaca ggtcaaaaac cagggaaggt ttctgttgaa ggaaaagaag
    25381 tagaggtcac aggttcgact gttaccagct attgggctaa taaggcagga gcgccagcga
    25441 caagttatcg ctttgctatt ggtggaagtg atgcggatta tcagaatgct tggtctagta
    25501 ttgtggggag tctaccaact ccatccagct ccagcagttc aagtagtagt tctagcgata
    25561 gcagtaactc aagtactaca cgaccttctt cttcaagggc gagacgataa ttctctaaat
    25621 gaagtggcca atcaatggat tgccactttt ttcttttcgt gttacaataa gggtatgaat
    25681 cagtatcaga aaaagattgt taacggaaaa atttattcgc tcctatccgg cttaatatgg
    25741 ggaatctgtg gaattttagg agagtacttc tttactcatt atcaggtgtc ttcgggctgg
    25801 attacctcta tgcgtttgac actggcaggg agtcttgtac tcatttggtc tgcaatacaa
    25861 ttaaaatcgc aagtgctaga tatttggcga gacaagaaaa attacctgcc ctttttagcc
    25921 tatgctattt tggggatttt ttcagttcag tattttttct atctctgtgt agaatactca
    25981 aatgctacga cagcaactat tttacagttt attagccctg tctttatcct cttttacaat
    26041 cgcttggttt atcaaaaacg agcgtcaaaa agcgctgttt tctatgtttt ggttgccatg
    26101 ctgggtgttt gcttgatggc aacaaaggga gatctctctc agttatccat gacgccgcta
    26161 gctcttataa caggtttgct gagtgccatg ggtgttatgt ttaatgttat cttgccccaa
    26221 ccttttgcta agcgttatgg ttttgttcct acggttgggt gggggatgat tttggcaggt
    26281 ttgtttagca atgtcctctc gccggtttat cagctttcct ttactcttga tatttggagt
    26341 atcttgattt gcctcattat cgctttcttt ggaacggctt ttgctttttt catttccatg
    26401 aaggctgtgt ccttggtttc tcctttggtg gttgccgtta tcagtgccag tgaacctctc
    26461 tcttctgctc tcttgagtgt tttgttctta ggattagtag tggattggtc cctccttcta
    26521 gctatagcct tgattatttt acccatgata tttttgtcta tagaagaagc gaaagaaagt
    26581 agataaaaag tcttttctta attctaaaag tgtgctatac tagaatagtc aataaaacac
    26641 ggggagatag ttgtgaagag tgtttttagg ttgtatcggt aggggcttgc ttttaccgac
    26701 agcacgtttt atctcacatc cctaaaaaat catacctaaa aacagacaaa aaggtttcaa
    26761 atttgaggcc ttttttggta gaataggtat cattataacg aaccaggagg cacctatgac
    26821 tgctacaaaa atgaacgctc aagaaattat ccaatttatc gccaatgctg aaaagaaaac
    26881 cagtgtcaaa gtaacctttg aggggcaact cgcaactgct gtgcctagct ctgttgtcaa
    26941 actaggaaat gtcctattcg gagactggaa ggacgtggct ccgcttcttg aaggtttggt
    27001 agaaaatcaa gattatgttg tcgagcaaga tgctcgtaat tctgcagttc ctttgctaga
    27061 taagcgtgct atcaacgctc gtatcgagcc aggtgctatt atccgtgacc aggtggaaat
    27121 tggtgacaat gctgttatca tgatgggagc tgttatcaat atcggtgctg aaatcggtgc
    27181 tggaaccatg attgacatgg gtgccatcct tggtggccgt gccatcgttg gaaaaaatag
    27241 ccacgttggt gcaggtgcag ttttggcagg tgtgattgag ccagctagtg ctgaaccagt
    27301 ccgtgtcgga gacaatgttc ttatcggtgc taatgcagtg gttatcgaag gagtccaaat
    27361 cggtagtggt tcagttgtcg ctgcaggagc tatcgttacc caagatgtcc cagaaaacgt
    27421 ggtagtagca ggtgttccag ctcgtatcat caaagaaatt gatgcccaaa cccaacaaaa
    27481 aacagcgcta gaggatgcgc ttcgtacttt gtaattgtaa aagtaaaaaa gaggcggaac
    27541 cctttttcca gcctctttct gctatatcgg aggacagata gatgttagat ttgattcaga
    27601 ctagacgagc tttacaccag attccagaga ttggcttgga ggagttcaag actcaggctt
    27661 atttgctgga tgtgattgag aaattgacta cgggcaagga ttttgttcaa attcgtactt
    27721 ggcggacagg gattttggtc tacctgcagg gaagtcagcc ggagcgaacc attggttggc
    27781 ggacagatat tgatggcctg cctatcgtcg aacaaacagg tctacctttt gcttctcaac
    27841 accaaggtcg catgcatgcc tgtggccatg attttcatat gaccattgcc ttaggctgtc
    27901 ttgagcgcgc ccttgaggag caacccaaga ataatctgct ctttctattt cagcctgctg
    27961 aagaaaatga agctggtggt atgctcatgt atgaggacgg tgcttttgga gattggttgc
    28021 caaaccaatt ctatggtctc catgttcgtc cagatttgaa agtcggccag attgcgacta
    28081 atactcatac actctttgca ggaacttgcg aggtgaagat ccgtttcaaa ggcaaaggtg
    28141 gccacgcagc ttttcctcat gaagccaatg acgccttggt ggcggctagt tactttgtga
    28201 cccaagtgca gtcagttgtt agccgcaatg tcaacccaat cgagggagca gtggtgacct
    28261 tcggcgtttt tcaagctgga accaccaaca atgtcatcac agacacagct tttttacatg
    28321 gaactattcg tgccttgaca caggacatga gtctcttggt gcaaaagagg gtaaaaacag
    28381 ttgcagaagg ggttgcagct gcctttgata tggaagtcga agtggaactc aaacaaggtg
    28441 gatacctacc tgttgagaac aatccagcct tggcgcgtga actgatggac ttctttgacg
    28501 aaaaagacgg aatcgagttg attgatatcg aacctgctat gacaggcgag gactttggtt
    28561 atctcctttc aaaggttgat ggcgtgatgt tttggctagg tatcgatagt ccctacgctc
    28621 ttcatcaccc tcaaatgagt cctaaggaag aagtcttagc tattggggtg gctgcggtgt
    28681 ctagtttcct gaaaaagaag gcagcagaat agagggcttg tctatgaaat cggaattacg
    28741 caagcaagtc ttgcatgaaa tgaaggcttt atctcaagaa caaaaacagg ctatagacca
    28801 agctttaacc gagcgaattt tacaacaccc cttttatcaa gaagccaagg tcatcgcaac
    28861 ctacctctct ttttctcatg agtttcaaac gcgggaactg attgagcagg cgctgaagga
    28921 cggcaagaag gttttaatac ccaaaactta tcccaagggg cgcatggact ttgtagtcta
    28981 tgatccgcag cagttggtaa aaactgcctt tgccttactg gagccacagg gagatttgga
    29041 agtggtggat gtatctcaga ttgatttgat tcatgttcct ggcctggctt ttacgacgga
    29101 tggatatcgg attggatatg gtggaggtta ttatgaccgc tatctggaac atttttctgg
    29161 tcatactttg agtacggttc atccttgtca aattcaggac tttatacctg aaaaccatga
    29221 tattcctgtt caggaggtat taattgatga aggaaatctt tgatagacgt taccctgtga
    29281 cgagtttctt cctcttagtg acggccttgg tatttttact aatgttggtc actgcaggcg
    29341 gaaactttga cagggcagat acattatttc gatttggagc catgtatggg ccagctattc
    29401 gcctctttcc cgagcaggtt tggcgtctct tgtctgccat ttttgttcat attgggtggg
    29461 aacatttcat tgttaatatg ctttcacttt attatcttgg aaggcaggta gaggagattt
    29521 tcggttctaa gcagtttttc tttctctatc ttttatcagg aatgatgggc aatctctttg
    29581 tttttgtatt tagtcctaaa tccttagcag caggcgcctc tacctctctt tatgggctat
    29641 ttgccgcgat tattgttctt cgctatgcaa ctcgcaatcc ttatattcaa cagctagggc
    29701 aatcctatct gacacttttt gtggttaaca ttattggaag tgttctgatt ccaggaatca
    29761 gcctagcagg ccatatcggt ggtgcagttg gtggcgcatt tctagcagtt atctttccag
    29821 ttagaggaga aaaacggatg tataatacca gccagagatt aggagcggta gtcttgttcg
    29881 taggactcgc cattttgctt ttctacaagg gaatgggaat gtgacgaagt tgaaagaaaa
    29941 tttgtgtaat gaaaaaagat aaggcatttt ttgaacctta tctttttatt ttattttatt
    30001 ccttctcagt caattctttt ccaagttgga tgaggtaatt cttcaaatca tctttgactt
    30061 gtgggtgttt gagggcgtag tcgatggatg ttttcatgaa gccaaacttg tctccgacat
    30121 cgtaacgagc cccattgaac tcacgagcaa atacacgttg tgttttattg agggtgtcga
    30181 ttgcatctgt cagctgaatt tcatttcctg cacctggagc ttggttttcg aggatttgga
    30241 aaatttcagg cgtgaggagg tagcgtccga taatagcaag gtcgctagga gcgtcctctg
    30301 gagctggttt ttcaacaaag gtttcaacac tgtaaagacc atcttttcct tcgccttgcg
    30361 gagcaataac cccgtaagca gatacttcgt catgagggac tggcatgaca gcgatagtag
    30421 acgcgtgggt acgctcgtag tcatccatga gttgtttggt aagtggaaca gccttttcgt
    30481 ctgtgatatc catcaagtca tcaccaagca taacgacaaa aggttcattt ccgacgaaag
    30541 ccttggcttg caaaacagca tctccgagac cgcgtggatg agtttggcgg ataaaatgca
    30601 gccgcatgtc agttgtttta tcaactagct tcaaaagatc tgttttccct ttttctttga
    30661 ggttatattc caattcgaag tttgaatcaa agtggtcctc aatagaacgt tttgatttac
    30721 cagtgacaac tagaatatct tcaatacctg atttgagagc ttcttccacg ataaactgga
    30781 tagttggttt gtctacgatt ggcaacattt ctttggcaag ggccttggtt gctggtaaaa
    30841 atcgagttcc tagtccagca gcagggatga ctgcctttct aacttttgat gtcataaaaa
    30901 tcctttctat agagggttaa gaccactcat tttctgcttt aaattcattg ttcatgatgt
    30961 cataaatggc atctttgata ttggttccgt gataaataac ttggtaaata gcctgtgtaa
    31021 tgggcatata gactccaagt tcttgggcta gttcataggc tgctcgagtc gttgaaattc
    31081 cttcgattac catgcccata ttagcttcta tatcagctag ggattctcct cgtccgagag
    31141 catctccagc tctccagtta cgagagtgga tggaagttcc cgttacgatc aaatctccca
    31201 caccagataa gccgctatag gtcaatggac tggccccgag tgctacccct aggcgggtga
    31261 tttctgctaa acctcgagcg atgatggctg ccttagcatt atcaccaaat ccaagaccat
    31321 gtaaagctcc agcaccgaca gcaataatat ttttaagagc accagcagtt tcaaccccga
    31381 taacatccgt attggtataa agtcggaagt agtgattact aaatagcttc tgaacgtatt
    31441 gagctgtttg taaatcttta gaagcagcag ttattaaagt taggtcacgc acaatggtct
    31501 cttctgcatg actaggccct gaaacaacga cgatatcact acggagatgt tcaggaattt
    31561 cttcttcaag aatggttgat aatcgtttat ggctatcagg ttctaatccc tttgatgcgt
    31621 gcatgatgat aaccttatgg tccaaggttt gtgcaacttg ctgggcaaca agtcgtgtca
    31681 cttttgttgg gacaacaaac aaaatcgcat ccacatcttt caatgtttct gctaagtcgg
    31741 tgtaggcaat gatattttcg tctagaacga catctttaaa gtagtgctta ttagtatggt
    31801 gtgtattaat ttcattgatt tgctcgggaa gatttcccca aatacgtacc tcgtgtccat
    31861 tgtcatttaa gacttgtgaa agggcggttc cccaagaacc aggccccaag acggcgacgg
    31921 tttgtttttc catgatttcc tccttgatag agttctttct cttattctat catattctag
    31981 gggattttgc acttgcatta ctggggtatg gtggaaaaat acacaaaaac cgagacgaag
    32041 ggataaactc ttagtcttta gttaaagtga gaagaaatct gatagatagt ttgattcagt
    32101 atatgcttat aatagaggta aacaactcag gaggttcttg taggttgcga gagtttggcg
    32161 aaaaaattaa aagattacgt ttggctaaaa aaatcagtcg ttcagaattt tgtggtgatg
    32221 agtctgaatt aagtatccgt caattaatta gaattgaaaa tggagaatcc agaccaatac
    32281 taacaaagtt aaaatatatt gctgaacgtt tggaggttga agattacaag ttgatgccaa
    32341 gttatataga gttggataag gaatacctag aattgaagta tttcttgatg aggactccta
    32401 catacgaaga tgaaactatc gcccaaaaga aagagagtgt tttgataaga tttttgaaga
    32461 gtattatgat aggctacctg aggaagaaag atttatcatc ccaaattatt catatctggc
    32521 actaacgaac tacacagttc aaaaattacc agaaaagcta gttgaaatac tgtccttctg
    32581 gtgattttgc ttaatttctg atgttcgaga cataattgag gagatttgtc ttgatttttt
    32641 cctaaaaaag ggcagactcc tcccttggtt tgatgctcga tttttaaaat aaggtgactt
    32701 attctgttag gcatgttttc ccctgccctc tagctctata tggaacaggg agtatcagat
    32761 ttatctggca gattgtatca aataaggctt gaaactgttt tgaataggca tatagacttg
    32821 agaatttgag aatatgtcgt ctagcagtag agacatattt tccggcaata tgaaggaaga
    32881 gacgtcgaaa acgcttgata gtcaaggcct ttacttcatc accagctagc tgctttaaaa
    32941 agaggtagag attataagcc agacagccca tcatcatacg tacttcattc ttaatcatgg
    33001 tcgaactatc tgtcttatcc ccaaagaatc ctgctttcct ttctttgata aagttttcag
    33061 cattccctcg ttcacgatag aattgaaaca gctcctcact atctacactg cttaaattgg
    33121 tcaaatgaac ttttttacaa ccataaaaaa gagcacttaa tcgtactctt ctcttttaag
    33181 gattagttga attaattcaa atccactagt tctcctgttt ctaggtagac taggcgttca
    33241 caaatgttag caatgtaatc agcgaagcgt tccagatgcc ctatgatata aaggtattgt
    33301 gttccattgg gaattgaggt ttcttggtct ttcataagtc caatgatttc ctttgataag
    33361 gcataataat attggtcaat ctgttcatct ttttgagcaa tactaatagc ttttgaggct
    33421 tggtgcaaag gaaaggcaac caataaatcg gatagcatgc tgagggataa tttacccatt
    33481 tggtgtaact gttcttcgtc aggggctagt tgattttctt ttagttgcaa aacagcttta
    33541 gcaatgcctg ccatatggtc tcccatacgt tcaaggtctg aacaagaaga catgatgcta
    33601 atcacaaatc gaaggtcaga cacttgtggc tgctgcaagg ccaacaaacg ggcacaggtc
    33661 aattcgatag cgctttgacc ttggttgata gcatgatcct tattgataat tagctctgcc
    33721 atctccttgt ctttggaggc taaggccagt aaggtttttg aagctgtttc aaggacaagt
    33781 tgccctagtc ctaaaaagga ttgttctaat tcatgcaatt ctaagtcaaa ttgatttctc
    33841 ataggttttt ccttccttat ccgaaccgtc ctgaaatata gtcttctgtg cgctgatctt
    33901 ttggattggt aaacacgtca acggtgtctc caaattcgca aatttctcct gttaagaaaa
    33961 aagcagtttt atctgaaata cgtgaagctt gttgcatgtt atgggtaaca atgataatcg
    34021 tataatcctt ttttagttgc tgaatgaggt cttcaatttt taaagtggag atagggtcta
    34081 aggctgaagt cggctcatcc attaacagaa catcaggttc tactgctaaa gctcgcgcaa
    34141 tgcaaaggcg ttgctgctga ccgcctgata aggacatggc actcttttta agattatctt
    34201 tgacttcgtc ccaaatggct gcccctttta aagatttttc cactaaggca tctaattgtt
    34261 ttttgtctcg aataccatgt gtccttggac cataagccac gttatcatag atagacatgg
    34321 caaagggatt aggctgttga aaaaccatcc ctacacgctt gcgtagctga ttaaggttga
    34381 atttgctact ataaatatct tgctcatcta agaggacttg gccttcaata tggcaagaag
    34441 gaaccaaatc gttcatccga ttaagggttt ttagaaaagt tgatttgcca caaccagatg
    34501 ggcctatcaa ggcagtaatc tgtctttctg gtaattgaat cgaaatattt tttaaggctt
    34561 gaaaatcccc gtaaaataag tctaggtgtc tgactgaaaa tgttcccata ctaggaagct
    34621 cctttcacaa gtttccgaga taataagctt gatagagtat ttatcattaa aacagtaata
    34681 attaaaatca cgccggtagc ataggcttca ttgacatgta gcccctcact tgacagcata
    34741 tacatatgta gggctagaga acggcctgaa gacatgagac tacttggcgt attggtagag
    34801 gtacctaatg tatacatgag ggcagctgtt tcaccaacga tacggccaat agctagtatc
    34861 actccagcta aaatacctgg catggcaact ggtagaacaa ttctaaaaac agtccgtaac
    34921 ttacctgccc caagtccata acttgcttga cgcatgctat cactaacaga taaaagggct
    34981 tcttctgttg agcgaataat gactggcaac accatgataa ctgaggttaa gattcctgat
    35041 aacagagagt attgaaaacc taagaagact acaaagaaga gcatgccaaa cagaccaaaa
    35101 acaatggaag gaatcccaga caaggtatct gaggccaatc gcatgatttt aacacaaagg
    35161 gaatcttttt ttgtatattc cacaagataa aaaccagcaa aaatccctat gggcaaagct
    35221 aaaagaagag caccaaaaac cagaataacg gtggaaataa tcgctggcat aagggaaatg
    35281 ttctcagaag tataagtcca agaaaagagg gatagactta gatgaggtaa gcctttgatg
    35341 aggataaaac caatgattaa aaagagagag ccaaaggtta aagctgaaaa acaataaacg
    35401 agaagtttta gcaggtattt actcataaga tgattttcct ttcaagtagg caaagtaggc
    35461 attaatcaag agaataagga aaaagagaac tgctgaggtt gcaataaggg cttccctatg
    35521 ctgacctgat gcgtaagcca tttccagaac aatattggtt gttaaggttc tggttcctga
    35581 aaagagtcca cttggaataa ttggctggtt gcctgccacc aaaataactg ccatggtttc
    35641 acctactgcg cgaccgattc ctaaaataac tgctgataaa ataccagatc tcgcagctgg
    35701 caagatgaca ctaaaaatac tccgttcatg actagctcct agagccaagc taccagaata
    35761 atacgttttg ggaactgttc ggatagcaga ttctgacaaa ctgataatgg ttggcaaaat
    35821 cattattcct aatagtaacg aagcggttag gacactcatg ccatttccta aaaaggttct
    35881 aatccaaggc accaataatt gtaggccgaa aaaaccataa acaatagatg gaatggctgc
    35941 catcaagttg atagctgatt ttaagaagcc atagacgggc tttggacaat aataaaccat
    36001 aaacaccgat gtcaagatgc ctgttggcac cccaatcaca atcgctccta aggtaattaa
    36061 taaggaacca acgatcattg gtaaaatacc atagcttgcc ggaatgttcg ttggcgacca
    36121 atcactgcct aataaaaaac gggcaaagcc gtagttagcc atgaaaggta agccattact
    36181 aaaaataaag aaacagatta gcaaaatagc tacaacagct actgttgcac tcatgaaaaa
    36241 aattgcccta aaaactgctt ctttgaaggc ttgttttgtc acatcttgtc ctttctagtg
    36301 aagaaagtaa gggagatacg acacctccct acttgccttc tttatcttat tgtacgatga
    36361 aacgtctgca tctctttaga gatttatgga gcaaacattt tatttaatct tgtcccaggt
    36421 ggttaatttg ccactaaaaa cgtctgcaag ttcagccata ctgacttggc ttgccttatt
    36481 gtcattattg accacaacag caataccgtc taaagcaata gcatcatggg tgagactctt
    36541 accttcttca ggagttaatt ccctagaaac cataccaata tcagcggttt tctccttaac
    36601 agcggtaata cctgctgaag acccattaga ggtaatatca atcgtaactt ctggattttc
    36661 ttttttataa gcttctgcta atttttccat taaagaagat actgaagtgg aacctacaac
    36721 agacaacttg cctgataagt gttggcttgt atattctgtg gtttcagttt tagcttcaat
    36781 aaatttatta tctgtgacca cttgttgacc ttgtttggag tggataaagc tgataaaatc
    36841 ttgacctagc ttggaaagat tagaagacca aacaatgttg aagggacgtt gaagagggta
    36901 ttcaccatct aaaactgtgt ctcgactagc cttgacacca tcaatctcta aagccttgac
    36961 agatttcgtt aaagatccca aggagatgta gccgatagca ttagcattcc cttgaactgc
    37021 tgagagaaca ccttctgtac tattttgaat cacagctgtt ttggcagtgt tgtcaatttt
    37081 tttatcaccg tcttttttga gaatccctgt gatttctgtg aaggcacccc gtgtcccaga
    37141 gccattttct cgtgaaatca cctcaatcgt tcctgaagct gactgtttgg aagtagctga
    37201 ctgattgcca caggcaacaa gcccaaatcc tgataagcca atggctgcaa gagtaagtat
    37261 ttttttgaat ttcataataa tcacctttat ctctatgtat ttttcttgtg taggcttact
    37321 acatttatag tctaacaagt ctttgtaaag gtttatccct gattcatgta aagattgtgt
    37381 aaagaatcaa aaaaagccac ttttgaaaaa tggctgctcc taaaaatagc tttaaaaatt
    37441 attagtcctg tgcgaaagat tggttaggaa gaaaaatcgt gaagcaactg cctctgccaa
    37501 gctgactcgt caccgtgact tggccaccta ataattgact gagttctttg acaatggcaa
    37561 ggccaagacc agtgccacca gtttgtctgc ttcgaccttt attaactcgg taaaaacgtt
    37621 caaaaatacg atcctgctct aattgactaa taccaatccc tgtatctgat acagaaatct
    37681 taatgccttc gttcaccttt tgggtcttga cctcaatttt tcccccttgt tcagtgtaac
    37741 ggatggcatt ggataaaaga ttgagtaaga tttgggaaag taattgacta tctgatacga
    37801 gggtgacatc atctggcacc tgcaccttta gctgtaaatc cttcttcttg agctgaggtt
    37861 gcaagctttg agtcaaatcc tgtacaaatt ctgccaaaga aagggtcgtc cattgtatag
    37921 gcatttgttg agccttagat aaggtaagaa gatgctcaac aatatgctca agacgcaagc
    37981 tttctttgta aataatgtct agaaagtcat ccttgagcgc ttcttcttca gctgacatcc
    38041 ccttaatggt ttcagcaaag cccttaatcg aagtaactgg tgtcctcaat tcatgggagg
    38101 catttgagac aaaggctaaa tttaactttt cataagttct aatcgttgtt aaatcatata
    38161 gcaagacgag cacagcttcc acagattggg tggggctaaa aacgggaact gctgtcactt
    38221 ctaaaatcaa gtcaccctca tgaaacccac ttacttcttg ttttaacctt gttttttgat
    38281 caaaggcttg gtgaactaaa ttccgaatat ccatccgttt gaggtcatca agtgaactta
    38341 tgtcgccgtc cacatcggga aaataatgag gcagagagcg actggataat aacatctgac
    38401 cttgagcgga aactaaaaac gtccccatgg ttaggtgcga cagaagaacc tccattgttt
    38461 cggctaggtc cttgtattgc tgatcctgtt gggagacttt ggtttttagg ccagacacat
    38521 actgagccaa agactttaag tcttcttgcc ctttttctaa aaagtattca ctactggtca
    38581 agagaggttg gtgcaaggtc tcaaaagcaa cttcccattt ccaaaggcaa aagagccagt
    38641 agccacctag tcccaaagaa agggctagaa gaaagagacc gatgccttta ctgatccaag
    38701 ttaatgccat ccctgcaatc agaatgaggc taacacttag attgactagc caaaattgaa
    38761 ggtagcgttt catctataac tccttgaact tataaccata accccgaatg gttcgaataa
    38821 attgaggggc tttaggattg tcttcaattt tttccctcaa cttaccaata tgaacgtcca
    38881 ccaaacgtgt ttcctgccca aagtcatacc cccagatacg ttccaaaaga cgctctctag
    38941 tcagtgtcat gttgggatgt ttcataagat agagcaagag ttcaaattct tttggggtca
    39001 aactcagtaa cttattcgcc ttgtagactt catgacgctc agggtatact ttcaaggtcc
    39061 caaatagcca agaattgtca gcgatattat ctgaatcatc tccttcttgt tttcctttag
    39121 ttcgcctgag gacagccttg acacgcgcca gtaattctct agggctaaaa ggcttggtca
    39181 ggtagtcatc agcccctaat tccaaggcca aaaccttatc aaattcatca cttttcgcag
    39241 aaaccatcat aattggagtt ttgacgcctt tggctctcag ccgcttacaa acttctatgc
    39301 catctaattg tggtaacatg atatcaagca agataaaatc aaagggttct gtttctgcca
    39361 aagctaaggc cttccgtcca tttgttacca attgagtaga aaagccttcc ttacttaaat
    39421 ggtagtcaag caatttcaga atgtgttctt catcatccac taataagact tgttttgtca
    39481 tctattatct cctattggta acattataac acaattatca gaaatcctaa cattgctaaa
    39541 tcagattaaa tttgcttatc aagactagta tctggtcaaa cgctcaatca tctccttgtg
    39601 ctctggatag gtcgccagta gatctaccct ttcaaataat tcaaaatcct caaattcaaa
    39661 accaggagca acaagacaag aaaccagagc atcatcctta tcaactgttg atccccaaat
    39721 agtgccctta ggaacacagt agtgaagttg ttgccctttg gatatgtcca ggcctaaagt
    39781 gactgcttcg tagtgaccat ctgctgtaat catgtgaaca gtaagtgggg attctgcatg
    39841 aaaataccag atttcatctg ctgtcaatcg gtgaaaatgt gaaggattcg tttcttctaa
    39901 taagaaataa atactggtat aaagcgccct tcccttacca gcaaggttta tagtgtctga
    39961 agcttttttt gtttgtctaa aatagccacc ttcaacatgg ggagctaact ctagagttct
    40021 tatcaagtct tctttatccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt
    40081 tttacgattt caagaactcc tctcagttct gaggacacgg taatgattga tgcgacggaa
    40141 gtacaaatca atcgccctaa aagaagaatt agcgaatgat tctggtaaaa agaaatgcca
    40201 cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcgc
    40261 tgtgaactat tgtcatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc
    40321 tggtaaaatc ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca
    40381 aactccacgt aaatccagca aactcaagcc actaacagtt gaagatcaag cctataacca
    40441 tgcgctatcc aaggagagaa gcaaggtcga gaacatcttt gccaaagtaa aaacgtttaa
    40501 aatgttttca acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat
    40561 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt
    40621 aggtgaatta gtgaagcgtt taggcaagtg tctctggtta cgacgtcatg gcctctaaat
    40681 cgattatatt taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta
    40741 gcgtctaaca attaggaact ttagttccaa taactttaag attacgacgt tttaggacat
    40801 aaatcgatca tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat
    40861 ttggcgttag ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc
    40921 tggcgcttct actccaagca agcgaagggc ttcttttaga acgactgcgg ttgcgtagct
    40981 gagggctaga cggctgtcgc gttctgggct ttcatccaag atacgtgtat gtgcatagta
    41041 tttgttaaag gattgagcta ggctaattgc aaatttagca atgatagaag gttcaaagtt
    41101 atctgccgca cggttgataa tacgtgggaa gtcttgaatg agtttaatga tttcccagct
    41161 ttcagtatca ttcaagctat agttgccagc tgtttctggt ttgaaatcgg ctttgcgtaa
    41221 gatagattgg atacgagcgt aggcatattg aacgtaaggt ccagtttcac cctcgaagga
    41281 taccatagct tctaggtcga agtcgtatcc atttgtacgg tcggttttga ggtcatagaa
    41341 tttaatggct ccaaccccaa cagcatgtgc tacttggtct ttgttttcta gttcaggatt
    41401 tttagcctcg atttggacct tggcacggct aacagcctct gcaacagtag gctctagcaa
    41461 gatgacattc cctttacgag tagagagctt cttcccttct tttgtaacca gaccaaaagg
    41521 aacgtgggta atgtcgtcac tccaatcgta gcccatctct tgcaagacag ctttgagctg
    41581 tttaaagtgg gcagattgtt cttgaccaac gacatagata gatttagcaa attggtattc
    41641 gtttttacgg tagagggctg cagccaagtc acgtgtgata tagagagttg caccatcaga
    41701 cttcttgatg agggctggat gttcaattcc atatttctca agattcacaa cttgggcacc
    41761 ttctgattca agaagtagtc ctttttcaga aagaatgtct acaactgcat ccatcttatc
    41821 attgtagaag gcttctccgt tatagctgtc aaattcaacc ttcaattcat tgtaaaggcg
    41881 gttaaattcc actaaacttt catcgcggaa ccattgccaa agagcgagag cttcctcatc
    41941 tccattttca agtttacgga accattcgcg cgcttcttca tccaagctag ggtcattttc
    42001 agcttcagcg ttgatgcgga catagagttt aaggagttca tcgattggat gagcttttac
    42061 agcttcttcg tcgccccatt ttttgtaggc aacaatcaac atcccaaatt gtttacccca
    42121 gtctcccaaa tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa
    42181 gctatctccg ataacagttg aacgcaggtg gccaatagaa aatggtttag cgatattcgg
    42241 actagacatg tcgataacaa cattttcttg tttgccaata ttttggtcag catagtgttc
    42301 tttttcagtg gtaacagctt gcaatacttg agcagaaatg gcagatttat caaggaaaaa
    42361 gttaacgtaa ggtcctgttg cgacaacttt ttcaaaggct tggctgttca ttttttcagc
    42421 cagttcagcc gcaatcattt gtggtgcttt acgttcgact tttgcaagag aaaaagcagg
    42481 gaaagcaatg tctcccattt ctgagttttt aggggtttcc agtaacttta aaatagcctc
    42541 ttggtccagg ctatcaatga tgctagataa ttcgctagca atcaattctt ttgtattcat
    42601 taagagctcc tttttggact tttctactat tttatcacaa ttttaaagaa agaagaaaaa
    42661 atttttgaaa tctcctgttt ttttggtata atatggttat aaatatagtt ataaatatgc
    42721 acgcaagagg attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact
    42781 gaggagaaat taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt
    42841 gtgacgcaga caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa
    42901 aatgatatgg tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt
    42961 ttgtctcatc atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa
    43021 ttgggagaag cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta
    43081 ggaacagttg ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa
    43141 ctcatggaag atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct
    43201 caagacttat ttttgaaaag gagagacaga aaatggcgat agaaaagtta tcacccggca
    43261 tgcaacagta tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg
    43321 gtgattttta tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt
    43381 ccttaacgag tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc
    43441 attctgccca acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag
    43501 agcagatgga agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca
    43561 ttacgccagg gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt
    43621 ccatagaccg cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact
    43681 tttatgtgac aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg
    43741 ctcgagaagt ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc
    43801 agatgaatct ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt
    43861 tgcgattggc aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga
    43921 ctcagatgag ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct
    43981 tgcagatgga ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta
    44041 agaaacaagg cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc
    44101 tcttgcgttc ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag
    44161 aagtagtgca ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca
    44221 agggtgttta tgacattgag cgcttggcta gtcgtgtttc ttttggcaaa accaatccaa
    44281 aggatctctt gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag
    44341 aagggatgga gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt
    44401 tggagagttt gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg
    44461 gaattatccg gactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag
    44521 ggactagctg gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc
    44581 tcaagattga ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactag
    44641 gaaatgtgcc agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa
    44701 ccgaagaatt agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc
    44761 tcgaatacga aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac
    44821 aagctctagc ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg
    44881 aaacccagca tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag
    44941 ggcgccatgc tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc
    45001 agatggcaga agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta
    45061 cctatatgcg tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg
    45121 ctgaaagcgc ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg
    45181 acttggtttc gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt
    45241 cgcatgcgac caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt
    45301 atgacgggat ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta
    45361 agaccctctt tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact
    45421 tggtcaatgt ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga
    45481 ttgaaccggg accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc
    45541 cagcagacct tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag
    45601 agagtcctcc tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata
    45661 gggcagaaga gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac
    45721 ctatgcaggt tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac
    45781 tagttaatct agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat
    45841 aacatcacac aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt
    45901 ttaaggagaa agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccttggcttc
    45961 gctcctgatg acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga
    46021 agtattagac gccctcctta ctgggaaata tgaagctatt tatagtatcg gggcttggtt
    46081 gattggtgtg gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta
    46141 tattgcccaa ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt
    46201 ttcttatgct gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga
    46261 tatcaaccag attcagaacg ttgtcatgat gaccttccag attcttttca gacttcccct
    46321 cttgttcatc ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat
    46381 tgttctcatg gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc
    46441 tcgttttgcc aagtttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt
    46501 acgtggcgtt cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac
    46561 agaggtctca gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt
    46621 ggaacccttt atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc
    46681 gggaatggtt cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct
    46741 aagccagatt atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc
    46801 catgatttcc atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa
    46861 ggatatccca gatgaagagt tggttggaag ccttagcttt gaaaatgtga cctttaccta
    46921 tccaatggac aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat
    46981 ggttggtgta gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg
    47041 tctctttgat ccacaggaag gggctatcaa aatcggaggt aaggatattc gagaagtgag
    47101 tgaaggaacc ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg
    47161 aacgattgca gataacttga gacaggggaa gggaaatgct actctatttg aaatggagcg
    47221 cgcagccaat attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag
    47281 tccagttgaa gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc
    47341 gcgtgggatt gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga
    47401 tgccaaatca gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac
    47461 cattattatt gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa
    47521 tcaaggacga ttgattggtc aaggcacgca tgcagacttg gttgccaaca atgccgttta
    47581 ccgtgaaatc tatgaaacac agaaatgaaa gacaaactat aagaaaagtc aatagtttta
    47641 tctaaactat ttcttatttc aatttgatga tttggcgatg attttagagc acggcaaaaa
    47701 gctattgaaa aagtccattt tttcaaaggt aatcctgtgt taatttcaga aattacatca
    47761 ctttttgttc gtcaaatggc agctcttttt taggatataa aacagggttc ggataagttt
    47821 ttttgcaagg tggatgatgg ctacattgta atgttttcct tgttctaact tagtcttaag
    47881 ataggcctta aaagcaggtg aaaagcgagg gcatgctttg gcagcttgta tgagtaccta
    47941 ccgcagatga ggggaacccc gtttgaccat tcttcctgct aaatcaatct gacctgactg
    48001 ataaatagaa gaatccagtc cagcgaaagc ttgtaattga gcaggattat caaaggcatg
    48061 aatatttcga atctcggcta aaatgaccgc ccctaaacga ttctcaatcc cagtaaccgt
    48121 cgtgatgacc gagttgaact cagccatcaa gtcattgaca cattttttcg ccttgtcaat
    48181 gagcctcttg taatgtttga tgttttcatt acaagagata aaacgtttat gcgttatcaa
    48241 actcattacc aattgaaaca aaaagctgtg gttagatcct ttcggaaatt gtcaagcgat
    48301 tggaggaaat gaactaatcc acagcggctt attccaagta taccacttgg gctttggcag
    48361 tagctaactg cgctaaatat aatataagga ggagtaaaat gaagacagtt caattttttt
    48421 ggcattattt taaggtctac aagttctcat ttgtagttgt catcctgatg attgttctgg
    48481 cgacttttgc ccaagccctc tttccagtct tttctggaca agcggtgacg cagctagcca
    48541 atttagttca agcttatcaa aatggcaatc cagaacttgt atggcaaagc ctatcaggaa
    48601 tcatggtcaa tcttggcctg ctggttttgg ttctatttat ctctagtgta atatacatgt
    48661 gtctcatgac gcgcgtgatt gcagaatcga ccaacgagat gcgcaaaggc ctctttggta
    48721 agcttgctca gttgacggtt tctttctttg accgtcgaca agatggcgat atcctgtctc
    48781 attttaccag tgatttggat aatatcctcc aagcctttaa cgaaagcttg attcaggtca
    48841 tgagcaatat tgttttatac attggtctga ttcttgtcat gttttcgaga aatgtgacgc
    48901 tggctctcat caccattgcc agcaccccat tggctttcct tatgctgatt ttcatcgtga
    48961 aaatggcacg taaatacacc aacctccagc agaaagaggt agggaagctc aacgcctata
    49021 tggatgagag catctcaggc caaaaagccg tgattgtgca aggaattcaa gaggatatga
    49081 tggcaggatt tcttgaacaa aatgagcgcg tgcgcaaggc aacctttaaa ggaagaatgt
    49141 tctcaggaat tcttttccct gtcatgaatg ggatgagcct gattaataca gccatcgtca
    49201 tctttgctgg ttcggctgta cttttgaatg ataagtctat tgaaacaagt acagccctag
    49261 gtttgattgt tatgtttgca caattttcac agcagtacta ccagcctatt atccaagttg
    49321 cagcgagttg gggaagcctt cagttggcct ttactggagc tgaacgaatt caggaaatgt
    49381 ttgatgcaga ggaggaaatc cgacctgaaa aggctccaac cttcactaag ttgcaagaaa
    49441 gtgttgaaat cagtcatatc gatttttcat acttgcctga taaacctatt ttgaaagatg
    49501 tcagcatttc tgcccctaaa ggccagatga cagcagttgt tgggcctaca ggttcaggaa
    49561 aaacgactat tatgaacctc atcaatcgct tttatgatgt tgatgctggt ggtatttatt
    49621 ttgatggtaa agacattcgt ggctatgact tagatagtct tagaagcaag gtgggaattg
    49681 tattgcaaga ttcggtcttg tttagcggaa cgattcgaga caatatccga tttggtgtgc
    49741 cagatgctaa tcaggaaatg gttgaggtag cagcaaaagc aacccacatt cacgactata
    49801 tcgaaagttt gcctgataag tacgatactc ttattgatga tgaccagagc atcttttcaa
    49861 cagggcagaa gcaattgatt tcaatcgctc gaaccctgat gacagatcca gaagttctca
    49921 ttctcgatga agcaacttca aacgtagata cggtgacgga aagcaagatt cagcatgcca
    49981 tggaggtggt tgtagcaggt agaactagtt tcgtcattgc ccaccgcttg aaaaccattc
    50041 tcaatgcaga tcagattatt gtccttaaag atggagaagt cattgaacgt ggtaaccacc
    50101 atgaactttt gaagctaggt ggcttttatt cagaactcta tcacaatcaa tttgttttcg
    50161 aataagaaag aagttgtcct atgtgggcag ctttttcttg tccataaaaa atgtttatca
    50221 cagccttaaa aaaaacatat tagacgaaag tcattttgag tgatatgata ggactatcgt
    50281 tagtattcga aaggagaggc atcatggcta gaacggttgt aggagttgct gcaaatctat
    50341 gtcccgtaga cgcagaaggc aaaatcattc attcatctgt atcttgtaga ttcgcagaga
    50401 tcattcgtca agtcggtggt ctccctttag tcattcctgt tggtgatgag tcagttgtac
    50461 gtgattatgt ggaaatgatt gacaaactca ttttgacagg aggccaaaat gttcatcctc
    50521 agttttatgg agagaaaaag accgtcgaaa gcgatgatta caatctggtc cgtgacgaat
    50581 ttgaattggc actcttgaag gaagcgcttc gtcagaataa accaattatg gcaatctgtc
    50641 gcggtgtcca acttgtcaat gttgcctttg gtggaaccct caatcaagaa atcgaaggtc
    50701 actggcaagg actacctttc gggacatctc actctattga gacagtagaa ggaagcgtgg
    50761 tggctaagct atttgggaaa gaaagtcagg tcaactcagt ccatcgtcaa agcattaaag
    50821 atttggcacc taatttccgt gtaactgcta ttgattcaag agaccaaact attgaagcga
    50881 ttgagtctat cgacgagcac cgcattatcg gtttacagtg gcatccagag tttctggtta
    50941 atgaagaaga tggcaattta gaattatttg agtatttatt gaatgaactg taacgattgg
    51001 aacatctggt cgttctttct tttatttttt caaaattttt agaatgtggc attttacgca
    51061 aacgtttgaa ttctgataaa aattggagaa attcgacaaa aaaacttgaa aaaaacgaag
    51121 gtaagcgtta tgatagaaaa gaagaaatat tggaggaaga acatgtcaca tattaaattt
    51181 gattattcaa aagttttaga caaatttgtt gcaccacatg aagtggaata catgcaatca
    51241 caagtaacag cagcagatga attgatccgt aaaggaactg gtgctggtag cgacttcttg
    51301 ggatggttgg accttcctga aaaatacgac cgcgaagaat ttgaccgcat cttgaaagct
    51361 gctgagcaaa tcaagtcaga cagcgatgtt ttggttgtga tcggtattgg tggatcttac
    51421 cttggtgcaa aagcagcaat cgacttcttg aatcaccatt ttgctaactt gcaaacaaaa
    51481 gaagaacgca aagctccaca aatcctttac gcaggaaact caatctcatc tacttacctt
    51541 gctgacttgg tagagtatgt tgcagacaaa gacttctcag taaacgtaat ttctaaatca
    51601 ggtacaacaa ctgaaccagc gattgctttc cgtgtcttta aagaactctt ggttaagaaa
    51661 tacggtcaag aagaagctaa taaacgtatc tatgcaacaa ctgaccgcca aaagggtgct
    51721 gttaaggttg aagcagacgc taacggttgg ggaacatttg ttgttccaga tgatatcggt
    51781 ggacgcttct cagtattgac agccgttggt ttgcttccaa tcgcagcatc aggagctgac
    51841 ataaaagctc ttatggaagg tgcgaatgca gctcgcaaag actacacttc agacaaaatc
    51901 tctgaaaacg aagcttacca atacgcagct gttcgtaaca tcctttatcg taaaggctat
    51961 gcaactgaga tcttggtaaa ctatgagcca tcacttcaat acttctcaga atggtggaaa
    52021 caattggctg gtgaatcaga aggaaaagac caaaaaggta tctacccaac ttcagccaac
    52081 ttctcaactg acttgcactc acttggtcaa tttatccaag aaggaactcg tatcatgttt
    52141 gaaacagttg tccgtgttga caaacctcgt aaaaacgtga ttattcctac tttggaagaa
    52201 gaccttgacg gacttggtta ccttcaagga aaagacgttg actttgtaaa caaaaaagca
    52261 actgacggtg ttcttcttgc ccacacagat ggtgatgtac caaacatgta tgtgactctt
    52321 ccagagcaag acgctttcac tcttggttac actatctact tcttcgaatt ggcaattgcc
    52381 ctttcaggtt acttgaatgc tatcaaccca tttgaccaac caggtgttga agcttataaa
    52441 cgtaacatgt ttgcccttct tggaaaacca ggatttgaag aattgagcaa agaacttaac
    52501 gcacgtctat aatagaagaa aagagtggtt tgcccactct ttttactctc tttatccata
    52561 gaaattggac tcagccaaga cttgtgatat aatatagaaa gcaaaaaggc agacgcctag
    52621 ataataggag aaactatgtc aaaagatatc cgcgtacgtt acgcaccaag tccaacagga
    52681 ctactacaca tcggaaatgc ccgtacagca ttgtttaact acttgtatgc gcgccatcat
    52741 ggtggaacat ttctcatccg tatcgaagat actgaccgta aacgccatgt cgaggatggt
    52801 gaacgttcac aacttgaaaa ccttcgctgg ttaggcatgg attgggatga aagtccagaa
    52861 tcacatgaga attatcgcca gtctgagcgt ttggacttgt atcaaaaata cattgaccaa
    52921 ctattagctg aaggaaaagc ctataaatct tacgttacag aagaagagtt ggcagctgaa
    52981 cgtgaacgtc aagaagctgc tggtgaaaca cctcgatata tcaacgaata ccttggtatg
    53041 agtgaagagg aaaaagcggc ttatatcgca gaacgtgaag cagctggtat cataccaaca
    53101 gttcgtttgg ctgtcaatga gtcaggtatc tacaagtggc atgatatggt caaaggtgat
    53161 atcgaatttg aaggtggcaa tatcggcggt gactgggtta tccaaaagaa agacggttac
    53221 ccaacttaca actttgccgt tgttatcgat gaccacgata tgcaaatctc tcatgttatc
    53281 cgtggagatg accatattgc taatacacca aaacagctta tggtctatga agctcttggt
    53341 tgggaagctc cagagtttgg tcacatgacc ttgattatca actctgagac tggtaagaag
    53401 ttatctaaac gtgatactaa tacacttcaa tttatcgaag attaccgaaa gaaaggttac
    53461 cttccagaag cagtctttaa ctttattgct cttcttggtt ggaacccagg tggtgaagat
    53521 gagattttct ctcgtgaaga actcattaaa ctttttgatg aaaaccgcct cagcaagtca
    53581 ccagcagcct ttgatcagaa aaaattagac tggatgagca atgattatat caagaatgca
    53641 gaccttgaaa ccatctttga aatggcaaaa ccattcttag aggaagcagg ccgtttgact
    53701 gacaaggctg aaaaattagt tgagctctat aaaccacaaa tgaaatcagt agatgagatt
    53761 atcccattga cagatctttt cttctcagat ttcccagaat tgacagaagc agagcgcgaa
    53821 gtcatgacgg gtgaaacagt tccaacagtt cttgaagcat tcaaagcaaa acttgaagcg
    53881 atgacagatg atgaatttgt gacagaaaat atcttcccac aaattaaagc agtccaaaaa
    53941 gaaacaggta ttaaagggaa aaatcttttc atgcctattc gtatcgcagt ttcaggcgaa
    54001 atgcatgggc cagaattacc agatacaatt ttcttgcttg gacgtgaaaa atcaattcag
    54061 catatcgaaa acattctaaa agaaatctct aaataaagag gatacaataa tggacatctg
    54121 ggaaaagatg tacgaagaag cacagaaact atacaatcca catgaagtat cagactttgt
    54181 ttatgccaat catgttgtag ctgcagtaga agcaggagat ggacaaatat ttacaggatt
    54241 ctgtatggag ggaacctgtg gtgttttcca tctctgcgca gaacgggcgg ccctcttcaa
    54301 tatgtaccaa ttttcaggac aaactaaggt taagaaagta ttagcctttc gagataaacc
    54361 accttatggt ggaagttcag ccatgccttg cggtgcttgt agagaattcc ttttagagct
    54421 taatgctgaa aataaagatg cagaattcat gatggactat aatataagaa aaacagttaa
    54481 agtctcagaa ctaatccctt attggtgggg agaagaacgt gcttctaagt ttaatgagcg
    54541 atagaagagt cagtataatt ctggctcttt ttactataag ttgaaaaaat aaaaaaaatt
    54601 agaaaaagta gttgacaaa
//
LOCUS       DAVKLW010000005        53327 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKLW010000005 DAVKLW010000000
VERSION     DAVKLW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.005"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            271..2250
                     /locus_tag="VW324_000267"
     CDS             271..2250
                     /locus_tag="VW324_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9725964.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2317..3813
                     /locus_tag="VW324_000268"
     CDS             2317..3813
                     /locus_tag="VW324_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9725965.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3813..4739
                     /locus_tag="VW324_000269"
     CDS             3813..4739
                     /locus_tag="VW324_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9725966.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4748..5815
                     /locus_tag="VW324_000270"
     CDS             4748..5815
                     /locus_tag="VW324_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9725967.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5826..6752
                     /locus_tag="VW324_000271"
     CDS             5826..6752
                     /locus_tag="VW324_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9725968.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            7319..8179
                     /locus_tag="VW324_000272"
     CDS             7319..8179
                     /locus_tag="VW324_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9725969.1"
                     /translation="MSQFITHQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELVRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            8191..9084
                     /locus_tag="VW324_000273"
     CDS             8191..9084
                     /locus_tag="VW324_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9725970.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            9097..9900
                     /locus_tag="VW324_000274"
     CDS             9097..9900
                     /locus_tag="VW324_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9725971.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(9933..10643)
                     /gene="treR"
                     /locus_tag="VW324_000275"
     CDS             complement(9933..10643)
                     /gene="treR"
                     /locus_tag="VW324_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9725972.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            10828..12795
                     /gene="treP"
                     /locus_tag="VW324_000276"
     CDS             10828..12795
                     /gene="treP"
                     /locus_tag="VW324_000276"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9725973.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            12973..14598
                     /gene="treC"
                     /locus_tag="VW324_000277"
     CDS             12973..14598
                     /gene="treC"
                     /locus_tag="VW324_000277"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9725974.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            14854..15102
                     /locus_tag="VW324_000278"
     CDS             14854..15102
                     /locus_tag="VW324_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9725975.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            15309..16103
                     /gene="racE"
                     /locus_tag="VW324_000279"
     CDS             15309..16103
                     /gene="racE"
                     /locus_tag="VW324_000279"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9725976.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            16100..17071
                     /locus_tag="VW324_000280"
     CDS             16100..17071
                     /locus_tag="VW324_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9725977.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            17047..17568
                     /locus_tag="VW324_000281"
     CDS             17047..17568
                     /locus_tag="VW324_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9725978.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            17565..18026
                     /locus_tag="VW324_000282"
     CDS             17565..18026
                     /locus_tag="VW324_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9725979.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            18017..18757
                     /gene="xerD"
                     /locus_tag="VW324_000283"
     CDS             18017..18757
                     /gene="xerD"
                     /locus_tag="VW324_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9725980.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            18757..19485
                     /locus_tag="VW324_000284"
     CDS             18757..19485
                     /locus_tag="VW324_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9725981.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            19504..20073
                     /locus_tag="VW324_000285"
     CDS             19504..20073
                     /locus_tag="VW324_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9725982.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            20060..20782
                     /locus_tag="VW324_000286"
     CDS             20060..20782
                     /locus_tag="VW324_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9725983.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            20782..21024
                     /gene="yidD"
                     /locus_tag="VW324_000287"
     CDS             20782..21024
                     /gene="yidD"
                     /locus_tag="VW324_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9725984.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(21249..22214)
                     /locus_tag="VW324_000288"
     CDS             complement(21249..22214)
                     /locus_tag="VW324_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9725985.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(22310..23062)
                     /locus_tag="VW324_000289"
     CDS             complement(22310..23062)
                     /locus_tag="VW324_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9725986.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(23059..24015)
                     /locus_tag="VW324_000290"
     CDS             complement(23059..24015)
                     /locus_tag="VW324_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9725987.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATSLVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(24005..24964)
                     /locus_tag="VW324_000291"
     CDS             complement(24005..24964)
                     /locus_tag="VW324_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9725988.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTNDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(25387..26088)
                     /gene="galE"
                     /locus_tag="VW324_000292"
     CDS             complement(25387..26088)
                     /gene="galE"
                     /locus_tag="VW324_000292"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9725989.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            26489..27553
                     /gene="pepA"
                     /locus_tag="VW324_000293"
     CDS             26489..27553
                     /gene="pepA"
                     /locus_tag="VW324_000293"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9725990.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            27575..27871
                     /locus_tag="VW324_000294"
     CDS             27575..27871
                     /locus_tag="VW324_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9725991.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            28553..28996
                     /locus_tag="VW324_000295"
     CDS             28553..28996
                     /locus_tag="VW324_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9725992.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            29000..29764
                     /locus_tag="VW324_000296"
     CDS             29000..29764
                     /locus_tag="VW324_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9725993.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            29779..30507
                     /locus_tag="VW324_000297"
     CDS             29779..30507
                     /locus_tag="VW324_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9725994.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            30500..32020
                     /locus_tag="VW324_000298"
     CDS             30500..32020
                     /locus_tag="VW324_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9725995.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVSKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            32365..33171
                     /locus_tag="VW324_000299"
     CDS             32365..33171
                     /locus_tag="VW324_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9725996.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(33338..33874)
                     /locus_tag="VW324_000300"
     CDS             complement(33338..33874)
                     /locus_tag="VW324_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9725997.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            34007..34897
                     /locus_tag="VW324_000301"
     CDS             34007..34897
                     /locus_tag="VW324_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9725998.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGAVVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLIISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSDLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            35141..35494
                     /locus_tag="VW324_000302"
     CDS             35141..35494
                     /locus_tag="VW324_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9725999.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            35529..36521
                     /locus_tag="VW324_000303"
     CDS             35529..36521
                     /locus_tag="VW324_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9726000.1"
                     /translation="MDLRPQIIEADDVIIRVVRACVCGSDLWRYRNPETKAGHKNSGH
                     EAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGSCDNHIGNNLGGDFQA
                     EYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHAARVANVQKGDKVVVI
                     GDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVVAERGQEGITKVREIL
                     GGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRALGSTFMQNISVAGGAA
                     SATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDERKTIKSMIVIE"
     gene            complement(36567..37574)
                     /gene="galR"
                     /locus_tag="VW324_000304"
     CDS             complement(36567..37574)
                     /gene="galR"
                     /locus_tag="VW324_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9726001.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            37738..38916
                     /locus_tag="VW324_000305"
     CDS             37738..38916
                     /locus_tag="VW324_000305"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9726002.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            38935..40416
                     /locus_tag="VW324_000306"
     CDS             38935..40416
                     /locus_tag="VW324_000306"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9726003.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGISLD"
     gene            40486..40890
                     /locus_tag="VW324_000307"
     CDS             40486..40890
                     /locus_tag="VW324_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9726004.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            41071..41835
                     /locus_tag="VW324_000308"
     CDS             41071..41835
                     /locus_tag="VW324_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9726005.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNYGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVKNG
                     QVRDPEKVTKEFKQSLFLRKNSLSVRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            41832..42128
                     /locus_tag="VW324_000309"
     CDS             41832..42128
                     /locus_tag="VW324_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726006.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESNYENLCVMVYTLSDPDC
                     VRWLSENMEFGDEKQLSLLNKKYSWEYGDELPEWLESPKHRLLLISELLERNLR"
     gene            42125..42292
                     /locus_tag="VW324_000310"
     CDS             42125..42292
                     /locus_tag="VW324_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HEU9726007.1"
                     /translation="MKKYTVEITETLTRLVSIEAENPDEAERLVREKYKSCEIVLDAD
                     DFQDDETSIYE"
     gene            complement(42339..43601)
                     /locus_tag="VW324_000311"
     CDS             complement(42339..43601)
                     /locus_tag="VW324_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9726008.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSTEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVVIILLINIFTKGFIKSISILIGLVVGTAIAASMGLVDFSPVAAAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKTRLPIYYAAGFLVLLGLLPKFGALAQIIPSP
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(43601..44182)
                     /locus_tag="VW324_000312"
     CDS             complement(43601..44182)
                     /locus_tag="VW324_000312"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9726009.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFASAGITKVVTIEASGIAPAIFTAEALNVPMIFAKKAKNITMNEGILTAEVYSFTKQ
                     VTSTVSIARKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVEAIGIVIEKSFQE
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(44312..44407)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(44632..45018)
                     /locus_tag="VW324_000313"
     CDS             complement(44632..45018)
                     /locus_tag="VW324_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9726010.1"
                     /translation="MDYQAVIPEFVVSDIEKSRHFYCDLLGFTIEYERPEENFLFLSL
                     EDCQLMLEEGTKDELVDLTYPFGRGVNISFGIVDVPQLHQKLLEADYPIHRPLTKREF
                     RVGDSFIYPHEFAVLDPDGYFLRFSE"
     gene            complement(45030..45857)
                     /gene="xth"
                     /locus_tag="VW324_000314"
     CDS             complement(45030..45857)
                     /gene="xth"
                     /locus_tag="VW324_000314"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9726011.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHLEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDKFFVTQVYTPNAGDGLKRLEERQVWDAKYAAYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRLADKVTKSDMIDSGARQDHTPIVLEIEL"
     gene            45991..46446
                     /locus_tag="VW324_000315"
     CDS             45991..46446
                     /locus_tag="VW324_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002281066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 domain-containing protein"
                     /protein_id="HEU9726012.1"
                     /translation="MKFYSYDYVLSQISQQNGIMIGFGIVLLAITGFFAFKAYRDKKG
                     TKFRELVMILALTLVAMLLVTISKYQTNQASNNQFQTSLHFIEVVSKDLGVDKSEVYV
                     NTSAATDGALVKVGSNVYRAMNGSQPDKYLLEKIELHQTDAIELVEVNK"
     gene            46443..47078
                     /locus_tag="VW324_000316"
     CDS             46443..47078
                     /locus_tag="VW324_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9726013.1"
                     /translation="MTLNYMEILIKLALGLFSLVFVINVTGKGNLAPNSATDQIQNYV
                     LGGIIGGVIYNSSISILQYAVILMMWTILVLTLKWLNNNVRFVKRLIDGKPTLLIKNG
                     QIDPEACRSVGLSAADVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVV
                     TDGVIQVDVLESIGRSEEWLLDNLSKQGHENVANIFIAEYDKGAVTVVTYE"
     gene            complement(47253..47330)
                     /locus_tag="VW324_000317"
                     /pseudo
     CDS             complement(47253..47330)
                     /locus_tag="VW324_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            47376..49121
                     /locus_tag="VW324_000318"
     CDS             47376..49121
                     /locus_tag="VW324_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9726014.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQVLLLALFYLLLAAFGMYYLRYVWRMYILGTSYRLGQIMRSRLFEHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNITQRGKVSYQRIEELLSQESPVQDPEFPLDGIENG
                     RLQYAIDSFAFENEETLTNVHFSLEKGQTLGLVGQTGSGKTSLIKLLLREYDVDRGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNSKLPLSAVEEATKLAQV
                     YQDIVAMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKEMRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            49111..50862
                     /locus_tag="VW324_000319"
     CDS             49111..50862
                     /locus_tag="VW324_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726015.1"
                     /translation="MRNKKEQWDVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAITVLLAYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNIYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQTEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLYLG
                     ITAGTMYAFIQYINRLFDPLIKVTQNFSTLQTSMVSAGRVFALIDERTYEPLQNDGQA
                     KIQEGNIRFEHVCFSYDGKHPILDDISFSVKKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGLVLLDDVDIRNYSQEELRKNIGLVLQDPFLYHGTIKSNISMYQEISDEQVKDAA
                     TFVDAASFIQDLPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            51035..51727
                     /locus_tag="VW324_000320"
     CDS             51035..51727
                     /locus_tag="VW324_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9726016.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            51770..52996
                     /locus_tag="VW324_000321"
     CDS             51770..52996
                     /locus_tag="VW324_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9726017.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
BASE COUNT        15339 a        10133 c        12216 g        15639 t
ORIGIN      
        1 gttataattt aatttgaatt ccaaaatcac attgttccgt ccgtatttca ttttactgta
       61 tgtcttccta agctccaaaa tttctgtgtc aaatttccta ttttcactgt agtctttgac
      121 acttcgaacg acaatttgaa tcatatattc aaaaaaaatg aaaaatttgt gccaacaagc
      181 ctaaatattc tgaaaatttg tttgattttt aatggataat gtgatataat ggttgtcaat
      241 ctataattca cataaacaga ggagtagaaa atgaaaaaaa atcgtgtatt tgctacagca
      301 ggtcttgttt tattagcagc aggtgtactt gcagcatgca gttcttcaaa atcatctgat
      361 tcatcagccc ctaaagctta tggctatgtt tatacagcag acccagaaac cttggactac
      421 ctgatttcaa gtaaaaatag tacaacagta gtgacttcaa atgggattga tggtttattc
      481 actaacgata attacggtaa tcttgctcct gcagttgcag aggattggga agtctctaag
      541 gatggtttga cctacactta taagattcgt aaaggggtta aatggtttac ctctgatgga
      601 gaagaatatg cagaggtgac ggctaaagat ttcgtgaacg gtttaaaaca cgcagcagat
      661 aaaaaatcag aagctatgta tttagctgaa aattcggtta aaggcttggc agattatcta
      721 tcaggaactt caacagattt ttcaacagtt ggtgtcaagg cggttgatga ttatacgtta
      781 caatacactt tgaaccagcc tgaaccgttc tggaactcta agttgaccta ttctattttc
      841 tggcctctga atgaagaatt cgaaacatca aaaggaagcg attttgctaa accaacagat
      901 ccgacatcct tgctttataa tggtccattc ttgttgaaag ggttgactgc aaaatcttct
      961 gtagagtttg taaaaaatga gcaatattgg gataaagaaa atgtccacct agatactatc
     1021 aatctagctt actatgatgg atcagatcag gagtcgctag agcgtaactt cactagtgga
     1081 gcttatagtt atgcccgtct ttaccctacc agctccaact attctaaggt tgcagaagaa
     1141 tacaaggaca atatctatta cacacaatca ggctctggga ttgctggtct gggtgtgaat
     1201 attgatcgcc aaagttacaa ctatacttct aaaactacag attcagagaa agtagctact
     1261 aagaaggcat tgcttaacaa agatttccgt caagccttga attttgctct tgatcgctca
     1321 gcttactcag ctcaaatcaa tggtaaagat ggagcagctt tagcagttcg taatttattt
     1381 gtaaaaccag actttgtttc agctggtgag aagacctttg gtgatttagt cgctgctcaa
     1441 cttcctgctt atggtgatga gtggaaaggt gtgaatttag ctgatgggca ggatggttta
     1501 ttcaatgctg acaaggccaa ggcagagttt gcgaaagcta agaaagcttt agaagcagac
     1561 ggcgttcagt ttcctattca tctggacgtt ccagtagacc aagcatcaaa aaactatata
     1621 tctcgtattc agtcctttaa acaatctgta gaaacagttc ttggtgttga aaatgtcgtt
     1681 gttgatattc aacaaatgac aagtgatgaa ttccttaata ttacttacta tgctgccaat
     1741 gcttcatctg aggattggga tgtatcggga ggagtttcat gggggccaga ctatcaagac
     1801 ccatctactt acctggatat tttaaaaaca actagcagtg aaactacaaa aacatattta
     1861 ggatttgata atccaaatag cccttcagta gttcaagttg gtttgaaaga atacgataaa
     1921 ttagttgatg aagctgccaa agagacaagc gacttgaatg tccgttatga aaaatatgca
     1981 gcggctcaag catggttgac agatagttca ctctttattc ctgctatggc ttcttctggt
     2041 gcagcaccag tgctttcacg aattgttcca tttactggag cttctgcgca aacaggctct
     2101 aaggggtcag atgtttactt caaatatttg aaattacaag ataaagcggt gactaaggaa
     2161 gagtatgaaa aagctcgtga aaaatggttg aaagaaaaag ctgaatcaaa tgagaaagct
     2221 caaaaagaat tggcaagtca tgtgaagtaa ataatttcta atagaacttt ctctccatga
     2281 ggagaaggtt cttttgggat ttttaaagga aataatatga aaaaatatat ttttatgcgt
     2341 gttttgcggt cattggtttc gattttctta gtaacgactt tgacctacac gattatctat
     2401 accttggttc ctcgaaaatt gattttcaag caggatccta actataataa aattgcgaca
     2461 acggctgata aacgtgataa ctatgaaaat actgtgtttg agcgtatggg ctacattgag
     2521 tattacgata ctaaagagtt gcaagaaaag gcaagtagca tggattcttc tgtaacagta
     2581 gaagcaaatg cgaccaataa agctatttat gaaaagtaca tcaatcaatt aggtcatggt
     2641 tggactttgg gagaatttac tgaaagtggt caattctatg ctactcgtga aattccaatt
     2701 tttgaacgtg tttttcactt ctatgctaac ttgattgaca ttgaccatac aaataaaatc
     2761 caagaccctg aaaatccaga cttgaaacgc taccttcgtt ttgaaaacga tccagctatc
     2821 ggatggtcat tggtcggttc aggaactaaa cataaatatc tcttgtactt taacagtcag
     2881 ttcccatttg ttcatcaaaa ctttgtgaac ttgaatttag gtgactctta cccaacctat
     2941 gctaatacac cagttcttca ggttattact caaggtcaag gacaaaccaa aactgcccaa
     3001 gttcagttcc caacaggtaa gaaaacgtct tctgtaaata tttactcaag aacctacaag
     3061 tcacctagtc aggctgactc tcgtgaagta gctagctatg ggaaagatga tccttataca
     3121 gcgactgaaa gtaattacca atatccatct atgattgtca gctctgctat tactggtttg
     3181 attggtttgg ttcttgccta tgctcttgcc gtgccacttg gttcagccat ggctcgtttc
     3241 aagaacactt ggattgatag cctctcaaca ggggctttga ccttcttgct tgctcttcca
     3301 acgattgcct tggtttacat cgttcgattg attggatcat ctatttccct tccagattca
     3361 ttccctatct tgggagctgg agattggcgt tcttacgttt taccagcagt catccttggt
     3421 ttgttgggtg ctcctggtac agccatttgg attcgtcgtt acatgattga cttgcaatct
     3481 caagactttg ttcgtttcgc tcgtgcaaaa ggtttgtctg aaaaagaaat ttcaaacaaa
     3541 cacatcttta aaaatgccat ggttccgctg gtttcaggaa ttcctggtgc cattattggg
     3601 gttatcggtg gtgcaaccct tactgaaaca gtcttcgcct tcccaggtat gggtaaaatg
     3661 ttgattgact ctgtaaaagc atctaataac tctatggtcg ttggtcttgt cttcatcttt
     3721 acatgtattt ctatcttctc acgtcttttg ggagatattt ggatgactat tattgaccca
     3781 cgtattaaat tgactgagaa aggaggcaaa taatgtctac aatcgataaa gaaaaatttc
     3841 agtttgtaaa acgtgacgat tttgcctctg aaactattga tgcgccagca tattcttact
     3901 ggaaatcagt gtttaaacaa tttatgaaga aaaaatcaac tgtagtcatg ttgggaatct
     3961 tggtagccat cattttgata agtttcatct acccaatgtt ttctaagttt gatttcaatg
     4021 atgtcagcaa ggtaaacgac tttagtgttc gttatatcaa gccaaatgcg gagcattggt
     4081 tcggtactga cagtaacggt aaatcgctct ttgacggtgt ctggttcgga gctcgtaact
     4141 ccatcctcat ttctgtgatt gcgacagtga ttaacttggt tatcggtgtt tttgtcggtg
     4201 gtatttgggg tatttcaaaa tcagttgacc gtgtcatgat ggaagtttac aacgtcatct
     4261 caaacatccc acctcttttg attgttattg tcttgactta ctcaatcgga gctggattct
     4321 ggaatctgat ttttgccatg agcgtaacaa catggattgg tattgccttc atgatccgtg
     4381 tgcaaatctt gcgctatcgt gacttggaat acaacttggc gtcacgtact ttgggaacac
     4441 caaccttgaa gattgttgcc aaaaatatca tgcctcaatt ggtatctgtt attgtgacaa
     4501 ccatgactca aatgcttcca agctttatct catacgaagc cttcttgtct ttcttcggtc
     4561 ttggattacc gattacagtg ccaagtttgg gtcgtttgat ttcggattat tcacaaaacg
     4621 taacaaccaa tgcttacttg ttctggattc cattgacaac ccttgtcttg gtatccttgt
     4681 cccttttcgt agttggtcaa aacttagcgg atgctagtga tccacgtaca catagatagg
     4741 agtagaaatg acaaaagaaa aaaatgtaat tttgactgct cgcgatattg tcgtggaatt
     4801 tgacgttcgt gacaaagtat tgacagccat tcgcggcgtt tcccttgaac tagtcgaagg
     4861 agaagtatta gccttggtag gtgagtcagg atcaggtaaa tctgttttga caaagacctt
     4921 cacaggtatg ctcgaagaaa atggtcgcat tgcccaaggt agtattgact accgtggtca
     4981 ggacttgaca gctttatctt ctcacaagga ttgggaacaa attcgtggtg ctaagattgc
     5041 gactatcttc caggacccaa tgactagttt ggaccccatt aaaacaattg gtagtcagat
     5101 tacagaagtt attgtaaaac accaaggaaa aacagctaaa gaagcgaaag aattggccat
     5161 tgactacatg aataaggttg gcattccaga cgcagataga cgttttaatg aatacccatt
     5221 ccaatattct ggaggaatgc gtcaacgtat cgttattgcg attgcccttg cctgccgacc
     5281 tgatgtcttg atctgtgatg agccaacaac tgccttggat gtaactattc aagctcagat
     5341 tattgatttg ctaaaatctt tacaaaacga gtatcatttc acaacaatct ttattaccca
     5401 cgaccttggt gtggtggcaa gtattgcgga taaggtagcg gttatgtatg caggagaaat
     5461 cgttgagtat ggaacggttg aggaagtctt ctatgaccct cgccatccat atacatggag
     5521 tctcttgtct agcttgcctc agcttgctga tgataaaggg gaactttact caatcccagg
     5581 aacacctccg tcactttata ctgacctgaa aggggatgct tttgctttgc gttctgacta
     5641 cgcaatgcag attgacttcg aacaaaaagc tcctcaattc tcagtatcaa agacacattg
     5701 ggctaaaact tggcttcttc atgaggatgc tccaaaagta gaaaaaccag ctgtgattgc
     5761 aaatctccat gataagatcc gtgaaaaaat gggatttgcc catctggctg actaggagga
     5821 aggaaatgtc tgaaaaatta gtagaaatca aagatttaga aatttccttc ggtgaaggaa
     5881 gtaagaagtt tgtcgcggtt aaaaatgcta acttctttat caacaaggga gaaactttct
     5941 cgcttgtagg tgagtccggt agtgggaaaa caactattgg tcgtgctatc atcggtctaa
     6001 atgatacaag taatggagat atcatttttg atggtcaaaa gattaatggt aagaaatcgc
     6061 gtgaacaagc tgcggaattg attcgtcgaa tccagatgat tttccaagac cctgccgcaa
     6121 gtttgaatga acgtgcgact gttgattata ttatttctga aggtctttac aatcaccgtt
     6181 tatttaagga tgaagaagaa cgtaaagaga aagttcaaaa tattatccgt gaagtaggtc
     6241 ttcttgctga gcacttgact cgttaccctc atgaattctc aggcggtcaa cgtcaacgta
     6301 tcggtattgc ccgtgccttg gtcatgcaac cagactttgt tattgcagat gagccaattt
     6361 cagccttgga cgtttctgta cgtgcccaag tcttgaactt gctcaaaaaa ttccaaaaag
     6421 agctcggcct gacctatctc ttcatcgccc atgacttgtc ggttgttcgc tttatttcag
     6481 atcgtatcgc agttatttac aagggtgtta ttgtagaggt tgcagaaaca gaagaattgt
     6541 ttaacaatcc aattcaccca tatactcaag ccttgctttc agcggtacca atcccagatc
     6601 caatcttgga acgtaagaag gtcttgaagg tttacgaccc aagtcaacac gactatgaga
     6661 ctgataagcc atctatggta gaaatccgtc caggtcacta tgtttgggcg aaccaagccg
     6721 aattggcacg ttatcaaaaa ggactaaact agtaatggtt ttataatttc catgtcaatt
     6781 tttatggaaa ttataaaact ttttttgttg gactgatttg aaaaactttg aaagcttcct
     6841 gaaagaattt ttgaaaaatt ttaaaaatta tgaaatgtta ttgacaggtg atgaaaaagg
     6901 tggtaggaca ggaagcatca aaaaagaaag cagaaaaaga aatgaataag agacaagccg
     6961 taactatgaa tcaaaacttt tactttagct actttagata gtgtttgtag acatgtcacc
     7021 atggatgcaa acagttcaag caatttgttt gtatctatgt ggctaagatt tttgattgtg
     7081 gtccatatag atgaatatct aaatggacta gctaagttgt aacttgttag gttatttcaa
     7141 gtatccgttt agatagtatc taggcggttt tttgttttat cttttctgag aaggagtttc
     7201 attatgaaag ttgttcgaaa atcactagcc ccctcttggt agtggggctc ctcttgactt
     7261 ctctgatcag tttgcatcag ttgaaggcag ataagaaaaa gatgtctttc gtatcggtat
     7321 ttcgcaattt attacccacc agtccttata cgctactaga gaaggatttg tggatgagct
     7381 ggccaaacaa ggctatgttg agggggaaaa tattgagatt gatttgcaaa atgcacaggg
     7441 agaacaaaga aatctaaaaa cgatttccca gcaactagca gaatccagcg atgtcgttct
     7501 agccatcgca aggccttctg ctcagagctt ggccaatacg acgcaaacga caccagttat
     7561 cttttcagct gtaacagacc cagtcagtgc caagttggtt gagtcaagag aacatcctgg
     7621 gggcaatgta actgggacaa gcgatcagtc atcagatgcc atttcaaccc aaatcaattt
     7681 gataaagaaa gtgttgccaa aggctaaaac gattggaatc ctctatactc agagcgagcc
     7741 caattcggtt gttcaaaagg atgaagctaa gcgacttctg aaagaaaaag gctttaccgt
     7801 tgttgaaaaa acaatcttgg acagtaataa cgtcaaggcg gcagcagaga gtttgatggc
     7861 agaggtggat atggtttttg tcccaactga taatatcatt tcgttaacca tggaaacagt
     7921 taagcaggtt tctattaaac acaaggttcc agtattcggt ggttcaacag aaatgattgc
     7981 tgttggtggc ttgtataact acggaaccaa ttatgaagaa ttggtacgtc agacagctcg
     8041 catgctgatt cgtgttttaa aaggtgagaa accagaaaat atagcagttg agttgcctga
     8101 aaaactggaa ttgcatacca atcaagaaat ggcagatgca ttgggaattg atattagtaa
     8161 gttagaaggc aaggaataag gaggattaag atgaattttg tgttatctag tttatcagaa
     8221 ggtttgttgt ggtcgattgt ggcgattggg gtctacttga ctttccgtat tttggatatt
     8281 gcggatatga ctgctgaggg agcctttcct ctgggggcag cagtcgttgt atcacagata
     8341 caggcagggg caaatccttg gcttgcgact ttacttgctt tgctggcagg tatggtagca
     8401 ggtcttgtat caggaatgct ccacaccaag atgaaaatcc cagctctctt gacagggatt
     8461 gtgaccttga cagggctcta ttcaatcaat attaaaatca tgggcagtgt acccaatctt
     8521 tccttgggag attctgcaac cgtctttaaa cagttggcga gcttgggtct gacaaatgaa
     8581 ggaactgttt tctcacttag tttggcctgt tttttacttg tttgtttggt cttgaccctt
     8641 ttgatgaaaa cagagattgg cttggtcttg cgttcgactg gggataatat cccgatgagt
     8701 gaggccaatg gagtcaatgt agacaccatg aagattgttg gttacatgat ttcaaacggt
     8761 ttgattgctc tatgtggttc cttgtttgcc caaaatgatg gattttcaga tgtgacttct
     8821 gggacaggaa ccattgttgt tggtttgagc tcggtgatta ttgcggaagt cttgatacac
     8881 gacttgacca ttggaggccg cttattatcc atcggaatcg gtgctattgt ttaccgtttg
     8941 attattttaa atatctatga aattccaaat ctagatcaaa acttggtacg tctctttaat
     9001 gcaatcttgc tcgctttggt tttatttgca ccagaattgc aaaagagatt aaagattcgt
     9061 ggtctgaaat tgagaaatga ataggaggag aaagttatga caagtttgtt aacacttgaa
     9121 aatattcaca agacatttga agcagggacg gtcaatgaga accatgtcct caaaggatta
     9181 gatttagagg ttgaagaggg agattttatc tctgtgattg gtggaaatgg agcaggaaaa
     9241 tccactttga tgaatatctt ggctggcaat ctatcggtgg acgaagggga ccttctactg
     9301 gcagggaaat ccattaaaaa tctgagtgtc agaaagagag ccaaggatat tgcccgtgtt
     9361 tttcaagatc ctaagatggg aacagcttct cgtttgacga ttgaggagaa tatggctatt
     9421 gccttgagac gcgggcagaa gagagggctt ggttggggcg tgaaggagaa ggatagaatc
     9481 cagttccaag aggccttgaa ggagttgaat attggtcttg aaaaccgctt aaaagtagat
     9541 actcaatatc tctcaggagg acaaagacag gctttgactc tagtcatgac agatctgatg
     9601 aaaccaaaac ttttactttt ggatgaacat actgcggcac ttgacccgaa aaccagtcaa
     9661 atggtgatgg acttgacgca aaagattgtg gagcaccatc agtggacgac tttgatgatt
     9721 acccatgata tgaatcatgc gattgagtac ggcaatcgtc tcattatgct ctatcaaggc
     9781 aagatagtgg tggatgtcaa gggagaagag aaaaagcatc tgacagttga agaccttatg
     9841 catctcttcc agaaaaatag tggccaaagt ctggtcagtg atgaattagt tttgggataa
     9901 agaaaaaggc tgagatctgg tgtctcagtc ttttatttct tgcgttttgc aaaatctaca
     9961 aatctaaatt tctctaactt atggcgactt tcggtaaact gaaattggcg tccgtcttgg
    10021 agatagactt ttgatttaat cgaaacgaca taagggtctt tgccaatgtc catgagaatc
    10081 ttgtctcggt cgcttgagtg gtcaatggtg atttccttct gagcataatc aataaggagt
    10141 ttgaggccat tttctatata agaatagata gactgctcag caatttggcg agtgagattt
    10201 ggaattagtt ccatatccag atagtccgta tccagaacgg ataccagatc atccaccaca
    10261 cgctggcgga ccaccttcca aaccatccga aactctggga aaccggttat cagtgaggat
    10321 tttttatcaa taataatctt gtccagactg accacgttgg ttttagagcg cagtccaagt
    10381 tctttaacta gttcttggta gctggttagg ttggatacag ggaaattgac ggtttcttct
    10441 ttgacgactt gagaaccttg ctctcttatc tttttgatca atccttcctc ttggaggaga
    10501 gacagggcct ttcggacggt atcacgactc acttgatatt gctccataag gtcgtgctcg
    10561 ctaggaagga aatctccgac agcgtaagtc tcgttttgaa tggtttcttg gatttgctta
    10621 aataattgtt ggtatttctt cattttgcct gctttaatag gtttttagtc tagtttaatg
    10681 aacacctgta tctattttac catacaagtg ctagaaaatt tacaaaaaaa cacccttttt
    10741 ataccaaaca agttgcagac aagtttggta tcgtttacaa tatacttatc aaatcaaact
    10801 tgctttgaca agtataagga gaatcaaatg ggaaaatttg aacaagaagc caaagatctg
    10861 cttcaggcaa ttggaggcaa agaaaatgtg actgccgtaa ctcactgtgc gacacggatg
    10921 cgctttgttt taggagatga taagaaggct aatgttaaag ctatcgagtc aattccagct
    10981 gttaaaggaa cttttacaaa tgcaggtcaa tttcaggtaa tcattggaaa tgacgtgccc
    11041 atcttttata atgattttac agccgtttca ggtattgagg gtgtttccaa agaagcagcc
    11101 aagtctgcag ctaagagtaa tcaaaacgtg gtccaacgtg ttatgaccac tctggcggag
    11161 atttttactc cgattattcc agccttgata gtcggaggat tgatcctcgg tttccgtaat
    11221 gtcttggaag gtgtccattg gtcgatgttg gatggcaaga ccatcacaga atcctctcag
    11281 ttttgggcag gtgtcaatca cttcctctgg ttgcctggtg aagctatctt ccagttctta
    11341 ccagtaggga ttacttggtc tgtttctcgt aagatgggaa ccagccaaat tttgggaatt
    11401 gttctcggaa tctgtttggt atcgcctcag ttgctcaatg cctatgcggt tgcttcaacg
    11461 ccagcagctg atatcgcggc aaactgggtt tggaattttg gctattttac tgttaatcgt
    11521 atcggttacc aagcccaagt tatcccagcc ttgcttgcag gtttgagtct gtcttatctt
    11581 gaaatcttct ggcgcaagca tatcccagaa gtcatttcta tgatttttgt acctttcttg
    11641 tcattgattc cagccttgat tttggctcat actgttttgg gaccaatcgg ttggacaatt
    11701 ggacaaggac tttcatcagt tgtcttggca ggtttaactg gtccagttaa agggctcttc
    11761 ggtgcaattt ttggtgccct ctacgctcca tttgtcatca caggtctgca ccatatgacc
    11821 aatgccattg atacacaatt gattgcggat gctggtggca ctgccctatg gccaatgatt
    11881 gctctttcta atattgctca aggctcagcc gtgtttgcct attatttcat gcatcgccat
    11941 gatgagcgtg aggctcaggt ttcacttcct gcaaccattt cagcctatct cggtgttaca
    12001 gaaccagctc tttttggggt taacgtaaaa tatatttatc catttgttgc tgggatgact
    12061 ggttcagccc ttgcaggcat gttatccgtt acttttaatg taactgcggc ttctattggt
    12121 atcggtggtt tgccaggtat tctctctatt caacctcaat acatgctgcc atttgcagga
    12181 actatgctag ttgcgattgt tgttccaatg ctcttgactt tcttcttccg caaggctggt
    12241 ctctttacaa aaacagaggg cgatacgaac ttgcaggcag aattcgttgc tcaagaagaa
    12301 gcagaatttg tgaaccatga accagtagaa cttacttcgg tagaaattat cagcccacta
    12361 actggccaag tgaaagaatt gagtcaagcg acggatccta tttttgcatc aggtgtcatg
    12421 gggcaaggtc tggtcattga accaagccaa ggtgagttga cctctccagt taatgggaca
    12481 gtgacggttc ttttccctac caagcatgcc atcggcattg tctctgacga gggagttgaa
    12541 ttgctcatcc acatcggtat ggatacagta ggtcttgatg gcaaaggttt tgaaagtctt
    12601 gtagtccaag gagatcacgt tacagttggt cagcaactga ttcgttttga tatggatgtc
    12661 attaaggctg caggtctggt gacagaaact cctgttatca tcaccaacca agatgcttat
    12721 acagcgacta ttcccggaac ttatccgaca acgattcaag ctggagcatc tctcatggtc
    12781 gctacacgaa tctaatactc ttcgaaaatc tcttcaaacc acgtcagctc tatctgcaac
    12841 ctcaaaacag tgttttgagc aacctgtggc tagcttccta gtttgctctt tgattttcat
    12901 tgagtataat tgaaatagca caaataggga cggaagactg cttcctccct tttatcagaa
    12961 aaggagaaac agatgacact tgataaagga aaagtagtct atcaaatcta tccaaaatct
    13021 tacaaagaca ccactgaaaa cggtattggg gatttccgtg ggattatcga aaaaattccc
    13081 tatttagcca aactaggcgt ggatatggtc tggctcaatc cattctatcc aagccctcaa
    13141 cgggataatg gttacgatat ttcagattat atggcagtgg atcctctttt tggtgatatg
    13201 gctgattttg aggaaatggt gtgtgtcggt aaagagcaca agattgactt tatgctggac
    13261 atggtactta atcattgttc gacagagcat gaatggtttc agaaagccct agctggtgat
    13321 aagtattatc aagacttttt cttcatccaa gaccaaccaa cagattggca gtctaagttt
    13381 ggtggttctg catgggcgcc tttcggggat actgggaaat actatcttca cctatttgat
    13441 gagacccagg ctgaccttaa ctggcgcaat tccaatgtcc gtaaggagct tttcaaggtt
    13501 gttaatttct ggcgcgacaa gggtgtcaaa ggtttccgat ttgatgtgat caatttgatc
    13561 ggtaaggacg aggtttcagt ggactgtcct gaaaatgaag ggaagccagc ttatacagac
    13621 aaacccatcg ttcataacta tttgcgtatg atgaaccaag ccacttttgg atctgacgat
    13681 agctttatga cagttgggga aatgtcttct accaccatgg aaaactgtgt cctctattcg
    13741 tcgcctgatc gtcaggaatt atccatgacc tttaattttc atcacctcaa ggtggactat
    13801 aaggatggac aaaagtggac cttggctccc tttgattttg aagagttgaa gagtctttac
    13861 catagttggg gtaaggaaat gagtgataaa gacggttgga gtgccctctt ttggaacaat
    13921 cacgatcaac cacgtgcctt gaaccgtttt gtcgatattc aaaacttccg caaggaagga
    13981 gccaccatgc tagcggccag cattcacctg tcacgtggga caccttatat ctacatgggt
    14041 gaggaaatcg ggatgattga cccagactat gattctatgg ctgattatgt ggatgtcgaa
    14101 tcgcttaatg cctatcagat gctcttagaa gaaggaaaga gtcagcaaga agccttccag
    14161 attattcaag ctaagtcgcg tgataattca cgcattccca tgcagtggga tgcttcggaa
    14221 aatgcaggat tttcaacagg cactccttgg ttgaaagctg gtaaatccta taaagacatc
    14281 aatgtagaaa atgaaatcca gggtccgatt tttaccttct atcaagactt gattcgactg
    14341 cgtaaggaaa tgccaatcat ttcagaagga agttacaaac cagcctttga agatagcaaa
    14401 caagtctacg cttttgaacg ccagtttgag gatcaaaagt tactagtgct caataacttt
    14461 tatgctaaag aagtggaaat cgacttgcca gcagtctacc aaaatggaca gattttgatt
    14521 tcaaactatg aagatgcaga agtgtctgag aaaattctac tcaaacccta tcaaacactg
    14581 gctatttatg taaattaata ctcttcgaaa atctcttcaa accatgtcag ttttatctgc
    14641 aacctcaaag cggtgctttg agcaacctgt ggctagcttc ctagtttgct ctttgatttt
    14701 cattgagtat aaacttagca aggagacctc tttttcagaa aagcagggtt ctttttgctg
    14761 tctattccat ccttgaaagt gtagaacaaa ctggtcaggg tggctagttt gtggtataat
    14821 gaaagggact caaaaagaaa caggagaaaa atgatggatt tacttttagc aattgtattg
    14881 attgtgctag cttttctagg aggagctctt ggaggaatgt acttggttcg taagcaaatc
    14941 gaaaaagaat ttgctgacaa cccacgtttg aatgctgaag cagttcgtac tcttttgagt
    15001 gcaaatggtc aaaaaccaag cgaagctaag gtacaacaag tttaccacca aatcatccgc
    15061 caacaaaagg cagcccttgc taacaataaa aagaaaaaat aaataaggaa aagtctggga
    15121 tgaaagttcc agacttctca ctatgttggt tatggtttag ggatgagact ttttccttgc
    15181 attctattga tatttgatta tgattaagag agaggtagtt gacattctat caagctgtcg
    15241 gtcagttcgt tcagtacaag gaatcataat gaacgatcaa tcagaaaaaa gactagaaag
    15301 aagactgtat ggataatcga ccaattggtt ttttggattc gggtgtcggg ggcttgaccg
    15361 ttgtgcgcga gctcatgcgc cagcttcccc atgaagaaat cgtctatatt ggagattcgg
    15421 cgcgggcgcc ctatggcccc cgtcctgctg agcaaattcg tgaatatact tggcagctgg
    15481 tcaactttct cttgaccaag gatgtcaaaa tgattgtcat tgcttgtaac actgcgactg
    15541 cggtcgtctg ggaagaaatc aaggctcaac tagatattcc tgtcttgggt gtaattttgc
    15601 caggagcttc ggcagccatc aagtccagtc aaggtgggaa aatcggagtg attggaacgc
    15661 ccatgacggt acaatcagac atataccgtc agaaaatcca tgatctggat cccgacttac
    15721 aggtggagag cttggcctgt cccaagtttg ctcccttggt tgagtcaggt gccctgtcaa
    15781 ccagtgttac caagaaggtg gtctatgaaa ccctgcgtcc cttggttgga aaggtggata
    15841 gcctgatttt gggctgtact cattatccac tccttcgccc tattatccaa aatgtgatgg
    15901 ggccaaaggt tcagctcatc gatagtgggg cagagtgcgt acgggatatc tcagtcttac
    15961 tcaattattt tgaaatcaat cgtggtcgcg atgctggacc actccatcac cgtttttaca
    16021 caacagccag tagccaaagt tttgcacaaa ttggtgaaga atggctggaa aaagagattc
    16081 atgtggagca tgtagaatta tgacaaataa aatttatgaa tataaggatg accaggactg
    16141 gtatgttggg tcttatagta tttttggtgg cgttaacagt ttgagcgact ataagacaga
    16201 ttttcctctg tttgaattct ccaaaatatt tggagatgaa gagtatggtt tcccgctttc
    16261 agttactgtt ttacgctatg gttctaccta ccgtttgttc tcctttgtgg tagatatgct
    16321 taatcaagaa atgggacgaa acttggaagt tattcaacgt catggggccc tgctcttggt
    16381 tgaaaatggg caactcttgt atgtagaatt gcctaaagaa ggggtcaatg ttcatgattt
    16441 ctttgagaca agcaaggtca gagaaacctt gttgattgcg actcgtaacg aaggcaaaac
    16501 caaggaattc cgagctatct ttgataagtt aggctacgat gtggaaaatc ttaatgacta
    16561 ccctgacctg cctgaagtag cagaaacagg tatgaccttt gaagaaaatg cccgccttaa
    16621 ggcagaaacc atttctcaat taacgggtaa gatggttttg gcagatgatt ctggtctcaa
    16681 agtcgatgtc cttggtggct taccaggcgt ctggtcagct cgtttcgcag gtgtgggagc
    16741 aactgaccgt gaaaataatg ccaaactctt gcacgaattg gccatggtct ttgaactcaa
    16801 ggaccgctcg gctcagttcc acacaaccct agtcgtagcc agcccaaata aggaaagttt
    16861 agttgttgaa gcagactggt caggttatat taactttgaa cctaagggtg aaaatggctt
    16921 tggctatgat cccctcttcc ttgtaggaga gacaggtgag tcatcagctg aattaaccct
    16981 agaagaaaaa aatagtcaat ctcaccgtgc cttagccgtt aagaaacttt tggaggtatt
    17041 tccatcatgg caaagcaaac catcattgta atgagcgatt cccatggcga tagcttgatt
    17101 gtggaagaag tccgtgatcg ctatgtgggc aaagtcgatg ccgtttttca taacggcgat
    17161 tctgaactac gtccggattc tccactttgg gagggcatcc gcgttgttaa agggaacatg
    17221 gacttctacg ccggctaccc agaacgtctg gtgactgagc ttggttcgac caagattatc
    17281 caaactcatg gtcacttgtt tgacatcaat ttcaactttc aaaagttgga ctactgggct
    17341 caggaggaag aggccgctat ctgcctctat ggtcacttgc atgtgccaag tgcttggttg
    17401 gaaggcaaga tcctctttct aaatccaggt tctatcagtc aaccacgagg taccatcaga
    17461 gaatgtctct atgctcgtgt ggagattgat gatagttact tcaaagtgga ctttttgaca
    17521 cgagatcacg aggtgtatcc aggtttgtcc aaggagttta gccgatgatt gccaaggagt
    17581 ttgagacttt cttgttgggg caggaggaaa cttttttgac ccctgctaaa aatctagctg
    17641 tgttgattga tacccacaat gcggatcatg cgaccctctt gctcagtcag atgacctata
    17701 cccgtgttcc cgttgtgaca gatgaaaaac agtttgttgg gacgattgga ctcagagata
    17761 ttatggctta tcagatggag catgacttga gccaagaaat catggcggat acggatatcg
    17821 ttcatatgac aaaaacggac gtagcggttg tttcgcctga tttcaccatt acggaggtct
    17881 tgcacaagct agtagatgag tccttcttac cggtcgtgga tgcagagggt attttccaag
    17941 ggattattac gcgcaagtcc atcctcaagg ccgttaatgc cctcttgcat gactttagta
    18001 aggaatatga gattcgatgc caatgagaga caggatttca gcctttttag aggaaaagca
    18061 gggcttgtct gtcaattcca agcagtccta taagtatgat ttggagcaat ttttagacat
    18121 ggtaggtgag cggatttctg agaccagtct caagatttac caagcccagc tagccaatct
    18181 aaaaatcagc gcccagaagc gaaagatttc ggcctgtaac caatttctat actttctcta
    18241 tcaaaaagga gaggtggaca gcttttaccg cttggaatta gccaaacaag ctgaaaagaa
    18301 gacggaaaag ccagagattc tatacctaga ctctttttgg caggaaagcg accatccaga
    18361 gggccgcttg ctagcgctct taatcctaga aatggggctc ttgcccagtg agattttagc
    18421 catcaaggtt gcggacatca atctggattt tcaggtgctg cgaatcagca aggcttccca
    18481 acagaggatt gtcaccattc ccacggcctt gctttcagaa ttggaaccct tgatggggca
    18541 gacctatctt tttgaaagag gagagaaacc ctattctcgt cagtgggcct ttcgtcagtt
    18601 agaatctttt gtcaaggaga aaggttttcc atccttatca gctcaagtct tacgtgaaca
    18661 gtttattcta agacaaatag aaaacaaggt cgatttgtac gaaattgcaa aaaaattagg
    18721 attaaaaaca gtcctgacct tagaaaaata tagataatgg atattaaatt aaaagatttt
    18781 gaaggacccc tggacttgct cttgcatctg gtttctaagt accagatgga tatctacgat
    18841 gtgcccatta cggaagtcat cgaacagtat ctagcctatg tctcaaccct gcaggccatg
    18901 cgtctggaag tgacgggtga gtacatggtc atggctagtc agctcatgct gattaagagt
    18961 cgtaaactcc ttccgaaggt agcagaagtg acagacttgg gggatgacct ggagcaggac
    19021 ctcctctctc aaatcgaaga atatcgcaag ttcaagctct tgggtgagca cttggaagcc
    19081 aagcaccaag aacgggccca gtattattcc aaagcgccga cagagttgat ttacgaagat
    19141 gcggagcttg tgcatgacaa gacgaccatt gacctctttt tggctttttc aaatatccta
    19201 gccaagaaaa aagaggagtt tgcacaaaat cacacgacga tcttgcggga tgagtataag
    19261 attgaggaca tgatgattat cgtgaaagag tccttgattg gacgagatca attgcgcttg
    19321 caggatttgt tcaaggaagc ccagaatgtc caagaggtca tcaccctctt tttggcaacc
    19381 ctagagttaa tcaaaaccca ggagctgatc ctcgtgcaag aggagagttt tggagatatc
    19441 tatctcatgg aaaagaagga agaaagtcaa gtgcctcaaa gctagacttg atagagagga
    19501 aagatgagta ctttagcaaa aatagaagcg ctcttgtttg tagcgggtga agatgggatt
    19561 cgggtccgcc agttagctga actcctctct ctgccaccga caggcatcca gcaaagttta
    19621 ggaaaattag cccagaagta tgaaaaggac ccagattcca gtttggcttt gattgagaca
    19681 agtggtgctt atagattggt gaccaagcct caatttgcag agattttgaa ggaatactct
    19741 aaggcgccta tcaaccagag cttgtctcgg gctgcccttg agaccttgtc cattattgcc
    19801 tacaaacagc cgattacgcg gatagaaatt gatgccatcc gtggagttaa ctcgagtgga
    19861 gccttggcaa agttgcaggc ttttgacctg ataaaggaag acgggaaaaa ggaagtattg
    19921 gggcgcccca acctctatgt gactacggat tatttcctag attacatggg gataaaccat
    19981 ttagaagaat taccagtgat tgatgagctt gagattcaag cccaagaaag ccaattattt
    20041 ggtgaaagga tagaagaaga tgagaatcaa taagtatatt gcccacgcag gtgtggccag
    20101 taggagaaaa gcagaagagc tgattaagca aggcttggtg acggttaacg gccaagtggt
    20161 gcgtgaacta gcaaccacta tcaagtcagg cgacaaggtc gaagttgaag gtcaacctat
    20221 ctacaacgaa gaaaaggtct actatctgct taacaaacca cgcggtgtga tttccagtgt
    20281 gacagatgat aagggtcgca agacggttgt cgacctcttg cccaatgtca aagagcgtat
    20341 ttaccctgtg ggtcgtttgg actgggatac atcaggtgtc ttgattttga ccaatgatgg
    20401 ggactttaca gacgagatga ttcaccctcg taatgagatt gacaaggttt atgtcgcgcg
    20461 tgttaaaggt gtggccaata aggacaatct ccgccccttg acccgtggtc ttgagattga
    20521 tggtaagaaa accaagccag ctgtttatga aattctcaaa gtggacccag tcaaaaatcg
    20581 ctctgtggtg cagttgacca tccatgaagg gcgtaaccat caggttaaaa agatgtttga
    20641 agctgttggt ctccaagtag ataagttgtc tcggactcgt ttcggacacc tagacttgac
    20701 aggactccgt ccaggagaat cccgtcgtct taataaaaaa gaaatcagcc aactacacac
    20761 catggctgta actaagaaat aatgaaacga attttaatag cgcctgtgcg cttttaccaa
    20821 cgttttatct caccagtctt tccaccctct tgtcgctttg agctgacttg ttccaactac
    20881 atgattcagg ctattgaaaa acatggtttt aagggggtat tgatgggctt ggctcggatt
    20941 ttacgttgtc atccctggtc gaaaacaggt aaggaccccg ttccagacca cttttccctt
    21001 aaacgaaatc aagaagggga atgaggtggg gtaaatagat ttcaaaatga taaaaacgca
    21061 tcctatcagg tttgagtaaa cttgatagga tgcgttttag aatgtcaaaa ttttatactc
    21121 ttcgaaaatc tcttcaaacc gcgtcagctt tcatctgcaa cctcaaaaca gtgttttgag
    21181 caacctgcgg ctagtttcct agtttgctct ttgattttca ttgagtatta aattgagttt
    21241 gaagtggctt atttcaaagc tttttgtatg tcttcaatca tgagttttgt tgattcaagt
    21301 ccgcctccgc ttagatacca gaggtctggt gttagttgga taatcttacc atttttagca
    21361 gcaggtgttt cagcgataag ggcattttct aggacaccgt cgttgctaga gttgtcccca
    21421 ccgatggcaa gggtacggtt gatgacaaag aggatgtcag ggttgatttc tttgacactt
    21481 tcaaagctga cttcttgtcc gtggcgtgag tcttcaaatt ttgtatcagt tggtttgaat
    21541 ttcaaggttt ggtacaagaa agagaaacga gatttggcac caaaggctgc catttttcct
    21601 tcattaagga ggatcgcaag ggcttttttg tcagagcttt catttttagt agcgacttct
    21661 tggatgctct tgtctagctt ggccaattct tccttggctt tctgtgtacc agtttcgccg
    21721 aaggcacttg ctaaggattc gatattagcc ttggtagaag tccagtagtc gtccttgctt
    21781 gcttggaaga gaacggttgg ggcgatttct ttgaatttgt ctacgaattt ttgtgtacgt
    21841 ggtgaagcga taatcaaatc aggctcaagg gcggcgatag cttctaaatc aggttctttc
    21901 atagaaccaa catttttgac agttcccact aggtctttta gataagtcgg aacagttttt
    21961 gtaggcattc cgacgatatt tttttcaaat cctaaagcgc gaatagtatc cgcagcgccg
    22021 aggtcaaagg tcacaatctt ttcaggaact ttggaaagtt tgacctcggc cagtgaactt
    22081 ttaatggtta cctctgttgg agcagagcta ctggtctctg tctgactagt gcttgagttt
    22141 gtactacatg caccaagtag gagcaagaag ctggccacta gggcagtgaa ataaagttta
    22201 agggatgttt tcataatttc tcctctttaa aatgtgataa cgatttaggg agtctcttga
    22261 tcttattgac taagagactg aaggttctct aacttgagct tttatgttac tagctataga
    22321 tacagatctt tttgtcattg atatcagcta gcgtgatggg aatctcataa agttgactga
    22381 gcaggtcagc ctgcatgatt tgatcggttc ttcccttgct aaagacctgg ccgtccttga
    22441 aggcgacaat ttcatctgca tactgactgg ctatgttgat atcgtggagg acgatgataa
    22501 tggtcttgcc gagttcctcc accagtcgtc gaagaatctg catcatgctg acgctttgct
    22561 tgatatcgag attgttgagt ggttcgtcca gcaagataaa gtccgtatcc tgggccagta
    22621 ccatagcgat aaagacgcgc tggagttgcc cccctgacag gctattgatg tagcggtctt
    22681 ttaagttggt cagttccaaa tagttcagag tttctcggat tttttcccag tcttctgatc
    22741 taagtcgacc tcggctgtag ggaaaacgtc caaaactgac cagttcttca acagtcaatt
    22801 tggcttggta attgattttc tgttttagga tggttagttc ttgggccagt tcttgcgaat
    22861 tccagctctc gatttcacgt cctttgatac tgagaactcc ctgatctttc ttggttagtc
    22921 tgctcatgat ggagaggaga gtcgattttc cagcaccatt tggaccaata aaggctgtca
    22981 gtttttgagg actgacttca agcgaaatgc cttgcaaaat atcctgtttt tgaatggatt
    23041 tgtcaatgtt ttccagtttc actgacgagc cctcctatat agtaagataa agaataagaa
    23101 gccacccaca ctctcaatga tcatactgat acgaatttcc agtgcaaaga ctcgttcaat
    23161 caaggcttgc cccaaggtta agctaataaa tccaaccaga atggccacta taaagagtaa
    23221 cttgtgctga tagtctttga caatcaggta ggtgaggttg gccagcataa agccgaagaa
    23281 ggccataggt cctaccaagg aagtggccgt tgaggtcaaa agcacgattc cccagaggag
    23341 ctctttctgt tctttttcaa catcgagtcc caatatctga gccgtttctc tttgcaggtg
    23401 caagacatct agaacgactg cttttcgaaa gaaaaagatt gtcaaagcga ggatgatcag
    23461 agaaccgatg gctaggatgg aagtgttgag atgttgaaag gaggcaaaaa gactattttg
    23521 cagtttatcg tattcgtttg gatccattag gacttgaagg aaggtgctga tatttcgaaa
    23581 gagacttctg agcgctagac agatcagcag gatgaagacc aggtcttgct tcatcagtgt
    23641 cttcaagtaa ccttgtaagg cgagaaagaa gagggactgg acaagaagta agactaggaa
    23701 ttctaagata ggggatttgc caagttgaag aaacttgctt tcaaaaacca gtagtagggt
    23761 ttgtagtagg acgtagaagg attcaattcc caaaatacta ggcgtcagga agcgattttc
    23821 cgtcagggtt tgaaaactaa tggtcgaaat cccagtcgcg atggctacca agagataaac
    23881 gatgatcttt tgggaacgca acttccaagc aaaggctgac aagtgagtga tgggccaaaa
    23941 gtagagaaga caagctccga tggcaagaat aatgagaatc cagaagagct tggtatgttt
    24001 gcttttagtc tgcatctttt cgtccccctc tccagagaag taggataaag acgagactac
    24061 cgatgattcc tagcaagaga ctgacagaca actcataggg cctaatcaga actcgggata
    24121 ggatatcgca agccagaact agattggcac caaccagtgc gaccatgagt ttggtttgac
    24181 ttagattatc tccatagcgc ttgcgaacaa gattgggaac gataactccg agaaatggta
    24241 ggccacccac ggtaatcatg gtgacgcttg tcgttagcgc caccagaaag agggccagtt
    24301 tttcaagtag ggagtaggaa atccccaaac tctcgctggt ttctttccct agattcatga
    24361 tggtgaaggt ttgggataat ttccaaacgg ttatcaggat gatgaggcct aagaagagcc
    24421 actcatactg atgggtctga atcatggaga aggagccctg ggtccaggca gtcatactct
    24481 gaaccagatt gaaacgatag gcgataactt ctgtgactga gccgataatc ccgctataga
    24541 tgatcccaat cagaggcaac atccaccttt cctttacagt aaaaatggtc ataaaggcta
    24601 ggaagaagag ggtgaatacg atggatgaaa caaaagcgaa gagcatcttt tgggtcagac
    24661 tagccgatgg aaagacaaaa aggctcagca ccattcccag tttggcggct tcagtcgttc
    24721 caactgtact cggtgcagca aactgatttt gggtaatagt ctgcatgaga aggcctgcca
    24781 tactcatact agaggcagtc aggagaatac tgatagttct tgggagacgg gactcttgaa
    24841 agaggagcca ggtctgctgg tcgaaatcaa atagctttcc ccatgaaaaa tcattggtcc
    24901 caatgctaat agagagaaag actaggagta gaagtaagcc aattaaataa tgagaaagtt
    24961 tcataccccg tcctttcatg tagatttggt atcgaaagat atctgcggat ataaatgtaa
    25021 cattattttt ctaatctgtc aataaaattt ctgacaattt aataaataca acaaggagag
    25081 agcaacaaga ctttctcctt tgttatccta ttctaaaatg tttttacctt aatctgataa
    25141 aataatatct tcgagggagt agctagccgt ccaatcaaga tattgtttag cttttgaagc
    25201 atctgctagg acactggctg ggtcactagc acgtcgagca acaatctcgt gtgggatttt
    25261 ttaatttagt aattcttcag cagttttaaa gatttctttg atagtatagc cttttttagt
    25321 tcctaagtta aagatttgag aagaactgtc ttcttgaaat aggtagttca ttcctttaac
    25381 atgagcctat gcaaggtcca agacataaat gtaatctcga atacatgaac cgtcacgtgt
    25441 atcgtagtca tctccaaata tttttaagct atcattttgt cccaatgcgg tcttgttgat
    25501 atttggaatg atgtgagttg gatttttcac acgcagaccg tttgaagcat ccatttcagc
    25561 cccagcaaca ttaaagtaac ggaaaataac atatttccag tcgtagcgat tggccatcca
    25621 gtaaatcatt cgttcgccca tcagttttgt ctctgcataa gggttgacag ggtcgagcag
    25681 ggtatcttca gtcaccggct tgtcaataca gttatttcca tagagagaag cagtcgaaga
    25741 gaacatgatt ttttgaatgc caacttcaga taagactttg agaacttggt tcataccagc
    25801 aacgttggca gtgaagtatt tacttggatt ttcaatactt tcgctcacaa caatttcacc
    25861 tgcacaatgg aggacagcat caatctgatt ttcttctaag taagccttta aggcgctagc
    25921 atcataaaca tccagttctt taaagctagc acgactatcc acagccgctc gatttcctgt
    25981 ggagagatta tcgaggacat gcaccttata gccagcattt aaaagagctt taacggtatg
    26041 ggagccaatg tagctagtcc cacctgtaac aagaattgtt ttggtcatga ttttttcctt
    26101 ttctagtttt gtactatagt aaattgaaat aagatatgaa caactctatt aggaaagtca
    26161 aattaatttc tagaaatgtt tcagcagcta cagcgtacta ttccaaactc aaccaactat
    26221 atatttaaag aaagaagaaa gggaagtcaa ctattttctg taaatttcat gaaaaatcca
    26281 aacaactttg atggtgtatt gtttttttgt ttggaaataa atattcgagt taattttgga
    26341 atgtttacta gattttgcac tacatgcaag agaatactat ccttttctaa gatagagcag
    26401 gactaagata ctaacttaga aatccacctt cccactatcc ttctcctata aaaagtggta
    26461 aaatggatga aagaaagaag gaactgaaat gacaacatta ttttcaaaaa ttaaagaagt
    26521 aacagaactt gctgcagtct caggtcatga agcgcctgtc cgtgcttatc ttcgtgaaaa
    26581 gttgacaccg catgtggatg aagtggtgac agatggcttg ggtggtattt ttggtatcaa
    26641 acattcagaa gctgtggatg caccgcgcgt cttggtcgct tctcatatgg acgaagttgg
    26701 ttttatggtc agcgaaatca agccagatgg taccttccgt gtcgtagaaa tcggtggctg
    26761 gaaccccatg gtggttagca gccaacgttt caaactcttg actcgtgatg gtcatgaaat
    26821 tcctgtgatt tcaggttctg ttcctccgca tttgactcgt ggaaaggggg gaccaaccat
    26881 gccagccatt gccgatatcg tttttgatgg tggttttgcg gacaaggctg aggcagaaag
    26941 ttttggcatc cgtcctggtg ataccatcgt accagatagt tctgcaattt tgacagccaa
    27001 tgaaaaaaat atcatctcaa aagcttggga taaccgctac ggtgtcctca tggtaagcga
    27061 gctagctgaa gctttatcag gtcaaaaact cggcaatgaa ctctatctgg gttctaacgt
    27121 ccaagaagaa gttggtctgc gtggcgctca tacctctaca accaagtttg acccagaagt
    27181 cttcctcgca gttgattgct caccagcagg tgatgtctac ggtggtcaag gcaagattgg
    27241 agatggaacc ttgattcgtt tctatgatcc aggtcacttg cttctcccag ggatgaagga
    27301 tttccttttg acaacggctg aagaagctgg tatcaagtac caatactact gtggtaaagg
    27361 cggaacagat gcaggtgcag ctcatctgaa aaatggtggt gtcccatcaa caactatcgg
    27421 tgtctgcgct cgttatatcc attctcacca aaccctctat gcaatggatg acttcctaga
    27481 agcgcaagct ttcttacaag ccttggtgaa gaaattggat cgttcaacgg ttgatttgat
    27541 taaacattat taaaccataa gggaggtggt agggatggta gaaccaaacc tagaaagcct
    27601 tataaaagat ctttacaatc atgctcgaca tgatttgagt gaagatttag ttgctgctct
    27661 cctagagact actaaaaaac tgcctactac aaatgagcaa ttgcaggcag ttcgtctctc
    27721 aagcctggtc aatcgtgaat tgctcctaaa tcccaaacat ccagcacctg agttgctcaa
    27781 cttggctcgc tttgtcaaaa gagaagaagc caagtacaga ggaactgcga cttctgcgct
    27841 tatgtatgag gaacttttta aaatgctttg attccattgt ggatcaaagc attttttata
    27901 agacctatct cgtgagcgtt caaatagtag taacaaggaa tcatgagaag tcagccgaga
    27961 ccatagtagt gaagaaactt ctgtaatgga agtggagtga agtgaggaat cgtcaaacgg
    28021 taggggatag gctgtttgaa gggtgctacg aaaaagatag ggtaaggtga aattatagcg
    28081 gaatgaaata agatatgaac aaattgatta gggaaatcaa atgaatttat agaaatcttt
    28141 tagcagtcat ggcgtcctat tctagtttca aaatgctata aatacgaaca aattgattgg
    28201 gaaattcaaa tcgctttcta tcaatgtttt agaagataca gtgccctagt ccaacttcaa
    28261 tatactataa aagaacagat ggaagcaatt atgatttact tctatcttta aaataaatga
    28321 tctgatagga aaaatgtgtc ccctcataaa aaataggata gtacggatgg aaggcttttg
    28381 tagactggag gacgaaggaa tttagaaact ctcgttaaaa gttagttgag ccggagtatg
    28441 gtggtgtgaa taattatcaa aaattatttc caaatcgaaa tagttgacag ttagaacgat
    28501 gagtgttaca attattttat tcatttcgat atcgaaataa attggaggtg ttatgaaaga
    28561 tagtcatttg ctagcccatc atattcgttt gttgaatggg cggatttttc aaaagttact
    28621 gagtcaagat cctgaggctc tttatagggg tgaacagggc aagattttag cggttttatg
    28681 gaatagtgaa actggctgcg caactgcgac agatatcgcg cttgcgactg ggcttgcgaa
    28741 taatacgctg acgactatga taaaaaagct agaggaacaa aagcttgtaa ttgttagtcc
    28801 gtgtggaaaa gacaagcgta agaagtattt agttttaacg gagttaggca agttccagaa
    28861 agaagtgggg catcgtgtca gtcagaaatt ggatactatc ttttacaaag gattttcaga
    28921 ggaagaaatt caccaatttg aaggttttca agaaagaatt ttggcaaatc tgaaagagaa
    28981 ggaaaatgag gtttagaata tgtcacaaca agtgaaaaat gctcacaacc tatacatcca
    29041 tgccattcaa gacggacgag ttgctgaggc tcaagcccag tctgtagggg atacctatat
    29101 ccaacattcg acaggtgttc ctgacgggaa agaaggattt gcagccttct ttgcagattt
    29161 ctttgagcgt catccagagc gtcagattaa gattgtccgc accattgagg atggcaatct
    29221 ggtctttgtt catgttcatc aatatctgaa tggtggagaa gctcaatggg tgacgacgga
    29281 tactttccgt gcggatgaga atggttgtat tgttgagcat tgggatgtca ttgactacta
    29341 tcgaacacct gaaaatgatc aactagatca aatttttgga gattttgaaa tcaaggattt
    29401 ggataagaaa gcagaaaata aaaagctggt tcgccgtttc ttgacagaaa ttttccaaaa
    29461 tggggagcta gagcagtgga gtgattatgt ggcagacgat ttgattcagc ataatcatga
    29521 gattggacaa ggaagtgctg cttataaaaa ctatgtggct gaatatagtg tcacttttga
    29581 ctttgttttc caactcttag gacaaggaaa ctatgtggtt agctatggtc agactcagat
    29641 tgatggcgtt gcttatgccc agtacgatat cttccgttta gagaacggga aaattgtgga
    29701 gcattgggat aataaggaag tcatgcctaa ggtagaagac ttgaccaatc gagggaagtt
    29761 ttaaattgag gacaaagaat gattgaatac aaaaatgtag cactgcgcta cacagaaaag
    29821 gatgtcttga gagatgtcaa cttacagatt gaggatgggg aatttatggt tttagtaggg
    29881 ccttctggat caggtaagac gaccatgctc aagatgatta accgtctttt ggaaccaact
    29941 gatggaaata tttatatgga tgggaagcgc atcaaagact atgatgagcg tgaacttcgt
    30001 ctttctactg gttatgtttt acaggctatt gctctttttc caaatctaac agttgcggaa
    30061 aatattgctc tcattcctga aatgaagggg tggagcaagg aagaaattac gaagaaaaca
    30121 gaagagcttt tggctaaggt tggtttacca gtagccgagt atgggcatcg cttacctagt
    30181 gaattatctg gtggagaaca gcaacgggtc ggtattgtcc gagctatgat tggtcagccc
    30241 aagattttcc tcatggatga acccttttcg gccttggatg ctatttcgag aaaacagttg
    30301 caggttctga caaaagaatt gcataaagag tttgggatga caacgatttt tgtaacccat
    30361 gatacggatg aagccttgaa gttggcggac cgtattgctg tcttgcagga tggagaaatt
    30421 cgccaggtag cgaatcccga gacaatttta aaagtgcctg caacagactt tgtagcagac
    30481 ttgtttggag gtagtgttca tgactaattt aattgcaact tttcaggatc gttttagtga
    30541 ttggttgaca gctctatctc aacatttgca gttgtcactt ttgaccttgt tactagctat
    30601 tttgcttgcg attcccttgg ctgtttttct tcgctatcat gagaagctgg ccgactgggt
    30661 cttgcagatt gccggtattt tccagaccat cccgtctctg gccttgttgg ggctctttat
    30721 ccctttgatg ggaattggga ccttgccggc tttgacagct ctagtgattt atgcgatttt
    30781 ccctattttg caaaatacta tcactgggct gaagggaatt gatccgaacc tgcaagaggc
    30841 tgggattgcc tttgggatga ccagatggga acgtctcaag aaatttgaaa ttccactcgc
    30901 catgcctgtt atcatgtctg ggattcggac ggcagctgtt ttgattatcg gtacggcaac
    30961 cttggcggcc ttgattggag cagggggact gggttccttt atcctgttgg gaattgaccg
    31021 taataatgcc agtttgattt tgattggggc actttcttct gcagtgcttg ctatcgcctt
    31081 taacttccta ctaaaagtaa tggaaaaagc aaaattacgg acgattttct caggttttgc
    31141 cttggtggct ttattactgg gtctgtctta tagtccagct cttttggttc aaaaagagaa
    31201 ggaaaacttg gttattgctg ggaaaatagg tccagaacca gaaattttgg ccaatatgta
    31261 taagttgctg attgaagaaa ataccagcat gactgcgact gttaaaccga attttgggaa
    31321 gacaagcttc ctttatgaag ctctgaaaaa aggcgatatt gacatctatc ctgaatttac
    31381 tggtacggtg actgaaagtt tgcttcaacc atcacccaag gtgagtcatg aaccagaaca
    31441 ggtttatcag gtggcgcgtg atggcattgc taagcaggat catctagcct atctcaaacc
    31501 catgtcttat caaaacacct atgctgtagc tgtttcgaaa aagattgctc aagaatatgg
    31561 cttgaagacc atttcagact tgaaaaaagt ggaagggcag ttgaaggcag gttttacact
    31621 cgagtttaac gaccgtgaag atggaaataa gggcttgcaa tcaatgtatg gtctcaatct
    31681 taatgtagcg accatggagc cagcccttcg ctatcaggct attcagtcag gtgatattca
    31741 aattacggac gcctattcga ctgatgcgga attggagcgt tatgatttac aggtcttgga
    31801 agatgacaag caactcttcc caccttatca aggggctcca ctcatgaaag aagctcttct
    31861 caagaaacac ccagagttgg aaagagttct taatacattg gctggtaaga ttacagaaag
    31921 ccagatgagc cagctcaact accaagtcgg tgttgaaggc aagtcagcaa agcaagtagc
    31981 caaggagttt ctccaagaac aaggtttgtt gaagaaatga tgggacagag actttaaaca
    32041 tactgaactc aacttcctta aacgactaga taaaagaatg ctcagacaat ttcgtctggg
    32101 cttttttgat gtgagaatga ctgaatgtca cttaaaatgg aaagaaagag aaaattttca
    32161 tgattttcta aaattttact gtaaagacag ttgactattc ataaaatagg tgtataatat
    32221 tttgtgaact acttaacaaa taaggggttt cagctcatgt cgactaaggt tagaaatctt
    32281 gtgtataggc agttcattat ctaatagagc gttgcgtccg aaagtctatc cagacacggc
    32341 tctttaaaaa caaaaggaga aatgatgcat acttatttgc aaaagaaaat tgaaaatatc
    32401 aaaacaaccc taggtgaaat gtcaggtggt taccgtcgta tggttgcggc tatggctgat
    32461 ttaggatttt caggaactat gaaggctatc tgggatgacc tctttgccca tcgtagtttt
    32521 gcccagtgga tttatttgct ggttttagga agttttcctc tctggctgga gttggtttac
    32581 gaacatcgta ttgttgactg gattgggatg atttgtagct tgacagggat tatctgtgta
    32641 atctttgtat cggaaggtcg agcaagtaat tatctttttg gcttgattaa ctctgttatt
    32701 taccttattt tggccctaca gaaaggcttt tatggtgagg tgctgacgac actttacttc
    32761 acagtcatgc agccaattgg acttctagtt tggatttatc aggcacagtt taagaaggaa
    32821 aagcaggagt ttgtcgcgcg taaactggac ggcaagggct ggacaaagta tctttccatt
    32881 agtgtgcttt ggtggttggc ctttggcttc atttatcagt ctattggtgc caatcgtccc
    32941 tatcgtgatt caatcacaga tgcaaccaat ggggtagggc aaatcctcat gacagctgtt
    33001 taccgtgaac agtggatatt ctgggcggct accaatgtct tttcaatcta tctctggtgg
    33061 ggagaaagcc tgcaaattca agggaaatat ctaatttatc tcattaacag tctagttggt
    33121 tggtatcaat ggagcaaggc agctaagcag aatactgatt tacttaacta ggaaaagatg
    33181 tttgaaagtg ctgttttgag atttcgatta aaacagatat agttgataat caaggattta
    33241 tagtatgaaa aagaggatcg gcgggtcctc ttttgttgtt gaaaagataa aaaactcagt
    33301 aacctagaaa taagacaact gaagctttac tctatattca atttttagga atgagaaggt
    33361 ctagataaaa ttggacaact tcctggtctg tgaaatcttg accttttttg agccaccagg
    33421 tcaatgtttc gataaagtta gttacgacaa aatgttggag gtaagaagta ggtagacttg
    33481 ggtgagtttg cttcaaatca gcagccagca tgggatagac gtggtgctca agctctctct
    33541 gtagttgacg gaggaaatag tcatttttgg aaaatagcag actggcgata tggtcttggt
    33601 ttttctgaaa atggagaaag aggtgggcga ggtagtcctc ggttgaaatg gcttgctctc
    33661 tttcaaaaag atgatggaag aggtagcggc agagctgatc cagaagaagc tctttactct
    33721 catagtgaca gtaaaaggtg gatcgtccca catctgcgag atcaatgata tcctgaacag
    33781 tagtggtctc gtagtcctta gcattcaaaa gttgtataaa agcttgatag atggcttttt
    33841 tggttttgct gatacggcgg tcaatgttag tcatatggac acttgaggca aattgttcag
    33901 aactgaataa agctgacgtt ttgcttctat cctttctttg agttttagtg gataatgata
    33961 atgaacaagg tgttcataaa tctattataa caaaggaatg agaaatatga aggcaaaata
    34021 tgctgtttgg gtggcttttt tcttaaattt gacttatgcc attgttgagt ttattgcagg
    34081 tggagtattt ggttctagcg ctgttcttgc tgactctgtg catgacttgg gagatgtgat
    34141 tgcaattgga atatcagctt ttctagaaac aatctccaat cgtgaagaag acaatcagta
    34201 caccttgggc tataagcggt ttagcctgct aggagccgtg gtaacagctg tgattctcgt
    34261 aacaggctct gttctagtca ttttggaaaa tgtcacgaag attttgcatc cgcaaccagt
    34321 caatgatgag gggattctct ggttaggaat tattgcgatt actatcaatc tgttagcgag
    34381 tctggtggtt ggtaagggaa agacaaagaa tgagtctatt ctgagtctgc attttctgga
    34441 agatacgcta gggtgggtag ctgttatcct gatggcgatt gttcttcgat ttacggactg
    34501 gtatatccta gatcctcttt tgtcccttat catttctttc tttattcttt caaaagccct
    34561 tccacgtttt tggtctacac tcaagatttt cttggatgct gtgccagaag gtcttgatat
    34621 caagcaagta aagagtgacc tggagcgatt ggacaatgtg gccagcctta atcagcttaa
    34681 tctctggact atggatgctt tggaaaaaaa tgccattgtc catgtttgtc taaaagaaat
    34741 ggaacatatg gaaacttgta aagagtctat tcgaattttc ctaaaagatt gtggttttca
    34801 aaatattacc attgaaattg atgctgacct agaaactcac caaacccata agcgaaaggt
    34861 gtgtgacttg gaacggagtt atgagcatca acattagaaa aaagtgaaaa atacttgggt
    34921 actatcttat ttggaataga gtaatttctt tattatttaa atatttcaaa aattggtaag
    34981 agaagagcat tgtataaact ccagatatat gattgttaat gataaaaatt tttcgattag
    35041 atacaaaatg cttgacttgg agtcaactca aagttatata ataagataag tgagttagaa
    35101 tagcgtgaat tcagtgaatg aaatgagagg aggttagcgt gtgaatatta aatctgccag
    35161 tgatttgttg ggaatttcag cggatacgat tcggtattat gaacgggttg gtcttgtgcc
    35221 accgattact cgtactgcta ctgggattcg tgattttcaa gatcaggata tcgaagcgct
    35281 ggaatttatt aagtgttttc gttcggcggg tgtctctgta gatagtttag ttgactatat
    35341 gtcgctctac caaaagggag atgaaacgag agaggagagg cttggtattt tagaagagga
    35401 aaagcaaaaa ttagaggagc gcttgtctca gctacagaca gctttaaatc gtttaaatct
    35461 caaaattaaa ctttataagg aaggaaaatt ttaaatgaaa tcagcagtat atacaaaggc
    35521 aggtcaggtt ggacttgcgt ccgcaaataa tagaagcgga tgatgtgatt attcgtgtgg
    35581 ttcgtgcgtg cgtttgtggt tcagatttat ggaggtaccg taatccagaa acgaaagctg
    35641 gacacaaaaa tagtggacac gaagcgattg ggattgttga agaagctggg gaagccatta
    35701 cgacggtgaa accaggtgat tttgtgattg tcccttttac acatggatgt ggtgagtgtg
    35761 atgcctgtct tgctggattt gacggttctt gcgacaatca tattggcaat aatttggggg
    35821 gtgattttca ggcagaatat attcgcttcc actatgcaaa ctgggcgctg gttaaaatcc
    35881 ctggtcaacc ttctgactat acagaaggga tgctcaagtc ccttttgact cttgcagatg
    35941 tcatgccgac aggctatcat gcggcgcgtg ttgcaaatgt tcaaaaaggg gacaaggttg
    36001 ttgttatcgg tgatggggct gttggtcaat gtgctgtcat cgcggctaag atgcgtggag
    36061 catcacaaat tatccttatg agccgtcatg aagaccgtca aaagatggct atggagtcag
    36121 gtgcgacagc tgttgttgca gaacgtggtc aagaaggaat taccaaggtg cgtgaaatcc
    36181 tcggtggagg agcagatgca gcacttgaat gtgttggtac ggaggctgct atagaacagg
    36241 cgctaggtgt tcttcataat ggagggcgta tgggctttgt aggagtccca cactataata
    36301 atcgtgctct tggttcgaca tttatgcaaa atatctctgt agcaggtggg gcagcttctg
    36361 ctacaacata cgataagcaa tttttactaa aagccgtcct tgatggtgat atcaatccag
    36421 gtcgcgtctt tacttcaagt tataaactgg aagatatcga ccaagcctat aaagatatgg
    36481 atgaacgtaa gacaattaag tctatgattg taatcgaata aaaaacgaat aggagtttta
    36541 gaactctatt cgttttttat gttatcctat tcttgattta gggtactttc tcttaatgtc
    36601 agtctggttc ccagcatggt caggctaggg attttccgac cgtggaggac ttccttgtta
    36661 agaatatcca tacctgctcg ccccatttct tcagtataaa ctgtgatgct ggagagggga
    36721 ggataaacct gcttggtcag gctggtgtcg ttaaaggaaa tgaggctgac acggtctggt
    36781 aggctgattc cagcttcttg gagcgcacga agggcaccga tggctaaact atcgctggct
    36841 gcgaaaaatg ctggtgggag ttggtctccc aagttctgaa tggcttcctt catcaagtca
    36901 tagccagact gggcagtaaa gtttccttga aagatcagtt catcatgata gattcctttt
    36961 gcttgactgt agtttctgaa attttccagc cgcttatcct caatgatttc ttcttggtca
    37021 gttgtttctt caaggcctgt tagaatcccg atacggtcca ttccttggct gaggaaataa
    37081 tcgacaacct gtttcatagc agtgtaaaaa tccgtgataa tacaggtatg tcccagggaa
    37141 agtgtatcgc tgtctagaaa tacaagaggc ttttggtatt cttcaaaggc agaaatctga
    37201 gctcggctaa attttccgat gcagagaatc ccaatcactt cctcgcttag ggtaaaagga
    37261 tggtcattaa aatagcgcaa gatatcgtag tccaactctt gggctctttt ttctattcct
    37321 aggcgaatct ggtagtagta gaggtcgtct agctcccctt gttcgctgac ccattgaata
    37381 atggcaatct tttgcttggg tttatgggac tcgcctgtct tgaggtgctt ggtgtagccc
    37441 agctcttcag caacggttaa aatacggtgt ctggtttctt ctgtaacaga taggctctgg
    37501 tcgcggttga ggacacggga tacggtcgcg atagagacag aggctagctg tgcaatgtct
    37561 tttaaggtag ccataaatcc tccttgatta ggttagtata tcatattttt cttcttttta
    37621 ctgatagttt actaaaattt tagtaaaaag gattgacctt gggaaattcc ttggatacaa
    37681 tagaagaaaa cgattacacg ttaagatggc ttaacggaca gtcaaaggag aattcatatg
    37741 gcacaacatc ttactactga agtccttcgc aaagactttc ttgctgtttt tggtcaagaa
    37801 gcagaccaaa ccttcttttc accaggtcgt attaatttga ttggtgaaca cacagactac
    37861 aacggtgggc acgtttttcc tgctgctatt tccttgggaa cttacggtgc agctcgtaag
    37921 cgtgacgacc aagtcttgcg tttctactca gctaactttg aggacaaggg cattatcgaa
    37981 gtgcctctcg ctgacctcaa gtttgaaaaa gagcacaact ggaccaatta tccaaaaggg
    38041 gttcttcatt tcttgcaaga agctgggcac gtgattgaca aaggttttga tttttatgtt
    38101 tatgggaata tcccaaatgg tgctggcttg tcttcttcag catccttgga gctcttgaca
    38161 ggagtcgtgg cagagcatct ctttgattta aaattagagc gtctcgattt ggttaaaatc
    38221 ggcaaacaaa cagaaaacaa ctttatcgga gtcaactctg gtatcatgga ccagtttgct
    38281 attggtatgg gggctgatca acgtgctatt taccttgata ccaacacctt agagtatgat
    38341 ttggtgccac ttgatttgaa ggacaatgtc gttgttatca tgaacaccaa caaacgccgt
    38401 gaattggcgg actctaaata caatgaacgt cgtgctgagt gtgaaaaagc agtggaagaa
    38461 ttgcaagttg ccttggatat tcagaccctg ggtgaattgg acgagtgggc cgttgaccaa
    38521 tatagctatc taattaaaga tgaaaatcgt ttgaaacgtg ctcgccatgc tgtgcttgaa
    38581 aaccaacgta cccttaaagc tcaagcagcc cttcaagcag gtgatttgga aacatttggt
    38641 cgtttgatga atgcgtcaca cgtttctctg gagcatgatt atgaagtaac tggtttggaa
    38701 ttagataccc ttgttcacac agcttgggca caagaaggag ttctcggtgc tcgtatgaca
    38761 ggggctggtt ttggtggctg tgccattgcc ttggttcaaa aagatactgt tgaggccttt
    38821 aaggaagctg taggcaaaca ctacgaggaa gtagttggat acgctccaag cttctatatc
    38881 gctgaagttg caggtggcac tcgcgtcctt gactagtcaa aaggaggctc tatagtgacc
    38941 ttagtagata aatttgtaac acatgtcatt tctgaaagtt catttgagga aatggatcga
    39001 atctacctga ccaatcgtgt tttggcacga gtgggagaag gtgttttgga agttgagacc
    39061 aatctggata aattgattga cctcaaggac cagctagttg aggaagccgt tcgattagag
    39121 acgattgagg atagtcagac tgcgcgtgaa atccttggta ctgaactgat ggacttggtg
    39181 actccttgtc cgagtcaggt caatcgtgac ttctgggaag cctatgccta ctctcctgag
    39241 caagcgatag aggattttta tcaactcagt cagaaaaatg actacatcaa actcaaggcc
    39301 attgctaaaa atatcgctta tcgtgttcca tctgattatg gagaacttga aattaccatc
    39361 aatctctcta agcctgaaaa ggatccgaaa gagattgcgg tagccaagtt ggtgcaagct
    39421 agtaattatc ctcagtgtca actttgttta gagaatgagg gttaccatgg tcgggtcaac
    39481 cacccagctc gcagcaacca ccggattatc cgttttgaaa tggttggtca ggaatggggg
    39541 ttccagtatt cgccctatgc ttactttaat gagcattgta tctttttaga tggccagcat
    39601 cgtcccatgg ccattagtcg tcagagtttt gaacgtctgt tggctatcgt agagcagttt
    39661 ccaggatatt ttgctggatc taatgccgac ctgccgattg tggggggctc tattctaact
    39721 cacgatcatt atcagggagg ccgtcacgta tttcctatgg aattggctcc cttgcaaaag
    39781 accttccgat ttgctggttt tgagcaggtc aaggctggaa ttatcaagtg gccaatgtct
    39841 gtcctacgct tgacttcgga ttccaaagag gatttgatta acttagctga taagattttc
    39901 caggaatggc gtcagtattc agattctagt gtgcagattt tagcagagac agacgggaca
    39961 ccgcatcaca ccatcacacc aatagcgcgt aagcgtgatg gacagtttga gttggacttg
    40021 gtcttgcgag acaatcagac ttcagcagag catcctgatg gcatctatca tccccacaag
    40081 gatgtccaac atatcaagaa ggaaaatatc ggcttgattg aggtcatggg cttggcaatc
    40141 ttgccaccac gtctgaaaga agaagtggag caagtcgcta gctatcttgt aggagaagct
    40201 gttacagttg ccgattatca tcaggagtgg gcagaccaac tcaaatccca acatccagac
    40261 ctaacggata aagaaaaagc ccttgcaatc gtcaaggact ctgtgggtgc tatctttgtg
    40321 cgtgtacttg aggatgcagg agtctacaag cagacagaac aagggcagac agcctttatg
    40381 cgctttgtgg aacaggtcgg aatttcacta gactaggagc tttctccttg cacagtccta
    40441 taaaaagtag tatcatagtg tcgtttgaaa tatcaggagg aaacgatgaa agactttcat
    40501 tttgacgcta tatctgcctt tgaaaattac gaaattgaaa aaatgagaga tggtcacgtt
    40561 gtggtgacga ccaaagtagt gaactcgtcg ctcaactact atggcaatgc ccatggtggc
    40621 tatctattca cgctatgtga ccaaatcagt ggtttggtgg ttatctcgct gggacttgat
    40681 ggggtgacac tccaatcctc tatcaactac cttaaggcag gaaaactcga cgatgtattg
    40741 accattaaag gagaatgtgt ccatcaaggt cgtacaacct gtgtgatgga tgtggatatc
    40801 accaatcaag agggcagaaa tgtctgtaaa gcaaccttta ccatgtttgt cacaggccaa
    40861 aggtctgagg aaagacgggt aaggatataa agaaaggtga gtgggataaa tttatctcac
    40921 tcatttttaa tttatgagat atgttagaag gaaatataaa agagagtggt gaaatacaaa
    40981 tgtaataggt acatatatgt ggtatactag ttatagtaga ttttaattgc ttatatcgag
    41041 tgagttctca tattagtgag gaatttttaa atggaattac actttaattt agaattagta
    41101 gaaacctata aaagtaatag ccaaaaggca cgtatcttaa cggaggattg ggtttatcgt
    41161 cagtcttatt gccctaatta tgggaacaat cccttaaatc attttgaaaa taatcggcct
    41221 gtagcagatt tttactgtaa tcattgtagt gaggagtttg aactaaagag caaaaaagga
    41281 aatttttcat caacaatcaa tgatggtgct tatgcaacga tgatgaagcg tgtgcaggca
    41341 gataataatc ctaatttctt ttttttaact tatacaaaaa attttgaggt aaataatttt
    41401 ctggtccttc caaagcaatt tgttacaccg aaatcaatta ttcaaaggaa gccacttgcg
    41461 ccaactgcta gacgagcagg ttggattggt tgtaacattg atttatcaca agtaccttct
    41521 aaaggaagaa tatttcttgt gaaaaatgga caagttagag atccagaaaa agttacaaaa
    41581 gaatttaagc aaagtttatt tttaaggaag aactctcttt cagtaagagg ttggacaata
    41641 gaaattctaa attgtataga taagatagag ggttcagaat ttacccttga agatatgtat
    41701 cgttttgaaa gtgacctaaa aaatatcttt gttaagaaca atcatatcaa agaaaagatt
    41761 aggcaacagc ttcaaatatt aagagacaaa gaaataatag aatttaaagg tagaggaaag
    41821 tatcggaaat tatgaaaacg aaacaacttg ttgcatcaga agaggtgtat gatttcttaa
    41881 aagtcatctg gcctgattat gaaacagaga gtaattatga aaacttatgt gtaatggttt
    41941 atactttatc agaccctgat tgtgtgaggt ggttatctga aaatatggag tttggtgatg
    42001 agaaacaact ttctttattg aataaaaaat atagctggga atatggagat gaattaccag
    42061 agtggttgga aagcccaaaa catagattat tgctaataag tgagttattg gagagaaact
    42121 taagatgaaa aagtatacag tagaaattac agaaactcta acccgtttag taagcataga
    42181 ggctgaaaat ccagatgaag ccgaacgact tgtaagagaa aagtataaga gttgtgaaat
    42241 tgttcttgat gcagatgatt ttcaggacga tgagactagt atatatgagt agatgttttt
    42301 attttgtcac acaaaaaaga ggctcacacc tctttttctt atttcttttt atgatttaat
    42361 acggcattga ggacaatggc aagtaggctg gctacgacga ttccgtttga gaagaacatt
    42421 tggaaggctg tcggcatgct gacaaagaga ttactgttgt tgagtccgac acctgcagca
    42481 attgatacag ctgcgataag gaagttgtgc tcattgttag caaagtcaac acgggcgagg
    42541 atttgcatcc cttgaataga cacaaaacca aacatcacca gcatggcacc accaaggaca
    42601 gggcttggaa tgatttgagc aagggcgcca aacttaggaa ggagtccaag gagaaccagg
    42661 aaaccagctg cgtagtagat tggcaggcga gtcttgatac ctgacaattt aaccaaacca
    42721 acgttttgtg aaaatcctgt gtaagggaag gtgttaaaga ttcctccgag aagtacggcc
    42781 aaaccttctg cgcggtaacc gttgcgaagg cgtgtgctat cgattgggtc tttcgtgata
    42841 tcagacaagg ctagataaac accagtagac tcaaccatag acaccgttgc gatgatacac
    42901 atcatgacaa tagatgagat ttcaaaggtt ggcatcccaa agtagagggg agttgggaca
    42961 tggacaagag gtgctgctgc aacaggagag aagtcaacca agcccatgct agcagcaatg
    43021 gcagttccaa caaccagacc aatcaaaata gagatagact tgataaatcc tttggtaaag
    43081 atattaatta agaggataat cacaacggta atagctgcaa gtagaagact ttgaccagtt
    43141 ggctctggaa cgttatttcc catatttcca atagcgacag gaatcaaggt taaaccaatc
    43201 gtggtaatga cagatcctgt tacgatagat gggaagagat tggccacttt tgagaagatg
    43261 cctgaaacaa gaaccacgta aatacctgat gcgataaggg caccaaacat agcaccacta
    43321 ccatggcttt gcccaatcat aatcaaggga gcgacggatt ggaaggcaac tccaagaacg
    43381 actgggagtc caatcccaaa gtatttgttg agttggagtt ggaggaaggt tgccacccca
    43441 cacatgaaga tatctgtgga aatcaggtag gtcaactgct cagttgaata gccaagggct
    43501 gtcgcaatca tgataggaac caggatggat cctgagtaca tggctagtaa gtgctgcaag
    43561 ccaagaacgg ctgcttgcga atgtttttct tgagtttgca ttagagatct gcctccttaa
    43621 atacgacttg accattttca aaacgatcca aacgagcgag tgatagaaca gggtagcctg
    43681 ctttttcaag caaatcacgg ccttcttgga aagatttttc aatcacgata ccgatagctt
    43741 cgactgtggc accagcttgt tcgatgattt gaatcaagcc tttggcagct tggccattag
    43801 caaggaaatc gtcgataatc aagaccttgt cctctggtga gaggaatttt ctagcgatag
    43861 aaacggtact ggtcacttgc ttggtaaagg agtaaacttc ggcagttaag attccttcgt
    43921 tcatagtgat gtttttagct tttttagcga aaatcatggg aacgtttaag gcttcagctg
    43981 taaaaatggc tggggcaata cctgacgctt caatggtcac gactttggta atgccagcag
    44041 aagcaaattt ttccgcaaaa accttaccaa tctctcgcat caagctaaag tcaacttggt
    44101 gggttaaaaa ggaatccacc ttgaggatgt tatcacccaa gatatgccca tccttgagga
    44161 tgcgctcttc taataatttc ataagacctc ctaaagtcta aaagccaatc catttgcttg
    44221 tttcagattt ctacccagta aaacggatag aaaaaggacc tacttaatct ctaagtaagt
    44281 ccccaaaata ggcatggcaa aaacgaccat acctcacttc tgacttactt attgttaggt
    44341 gttccggcac cttgtagaaa cgtcgtgcca attcacgaca taaacaagta aaacgatatt
    44401 caatttcaaa taggcgtgag ccaatgtttt tattttacac taaataactt tggaaatcaa
    44461 atgttttgtt agtgttttgg tttaaaaagc gaacgaatat aataaaaatg ggcaaaagtt
    44521 aacttattag ctaacattta gaaggttttt tcatcataaa aggacatatt attaggtttt
    44581 taaatgcctg acaatatgcc tttttctgat ttgaaggatt ttttctattc tctactcgct
    44641 aaatcttaaa aaatagccat ctggatccaa gactgcaaat tcatggggat atataaagct
    44701 atctcctact cgaaattctc tttttgtcag gggacgatgg ataggatagt cagcttccag
    44761 cagtttttgg tggagctgag gaacatctac aatgccaaag gaaatattga caccgcgtcc
    44821 gaaaggatag gtcagatcaa ctaattcatc ctttgttccc tcctctaaca ttagctgaca
    44881 atcttcaagc gataagaaga ggaagttttc ttctggtcgc tcgtattcga tggtaaaacc
    44941 cagcaaatcg cagtagaagt ggcgtgattt ttcgatgtca gatactacaa attcaggaat
    45001 gacagcttga tagtccattc gttttctcct tagagttcaa tctccaagac aatcggtgta
    45061 tggtcttggc gagcacctga gtcaatcata tcagatttag tgaccttgtc tgcgagacgg
    45121 ttacttgtga gccagtagtc gattctccag cctgtattgt tgattttaga agtcttgctg
    45181 cgttgtgccc accaagtgta gcgttctggg acatcgccgt gaacatggcg gaaggtatcc
    45241 gtaaatccag ttgccaagag attggtaaat ccagcacgtt cctcgtcggt aaatcctggt
    45301 gaacgacggt tgctggcagg atttgcaagg tcgatttcat tgtgggctac attgtagtca
    45361 ccggtcgcaa ggactggttt ttctttgtct agctcagcca agtacgcagc atatttggca
    45421 tcccagactt gacgttcttc caagcgtttg agaccatcgc cagcgtttgg agtgtaaact
    45481 tgggtcacga aaaatttatc aaattctaga gtgatgatac gaccttccaa gtccatggta
    45541 gaaggggcac cgatttctgg gaaagtgaca gtgggtgtga gttctttctt ataaaggaac
    45601 atggttccag catagccttt acgggcaggt tcttgggaag agcgccacgt attttcgtag
    45661 cctgggaaga gttcttctaa aatttccaag tgtttctttg taggaccctt agcagaaagc
    45721 ttggtttctt ggatagcgat aatatcagca ttttcagcga ccaaggtttg taggacttct
    45781 tgggacaatt tcgcgcgagc tgagtcacta gttagggcag cattgagaga atcaatattc
    45841 catgagataa gtttcataaa gttacctttt tcattcagat tatagatttt attataccaa
    45901 aaaaaggtct atttccccaa tgtatggttt gaaaaatcac cctctttcgt ttataattaa
    45961 gaatgatttt atgaaaagga gtgaaaataa atgaaatttt actcatatga ctatgtgctt
    46021 agccaaatca gtcagcagaa tgggattatg attggctttg ggattgttct cctagctatc
    46081 acaggatttt ttgcttttaa ggcctatcgt gataaaaagg ggactaagtt tcgggaattg
    46141 gtcatgattt tggccctgac tttagtggcc atgcttttgg tgacaatttc aaaataccag
    46201 accaatcaag cctctaacaa tcaatttcaa acctcccttc atttcataga ggttgtttcc
    46261 aaagacttgg gagtggataa atcagaagtt tacgtcaata cttcagctgc cactgatgga
    46321 gcgcttgtca aggtaggttc aaatgtctat cgtgctatga acgggagcca accagacaag
    46381 tatcttttag agaaaataga attgcatcaa acagacgcta ttgaattggt ggaggtaaac
    46441 aaatgacact caattatatg gaaattttaa tcaaactggc cttggggctc ttctctcttg
    46501 tttttgttat caatgtgaca ggaaaaggaa acctagcgcc taactctgcg acagaccaaa
    46561 ttcagaacta tgttcttggt ggtatcatcg gtggggtgat ttacaatagt tcaattagta
    46621 ttctgcaata cgcagtgatt ttgatgatgt ggacgatttt ggtcttgacc ctcaaatggc
    46681 tcaacaataa tgttcgtttt gtcaaacgct tgattgatgg gaaaccaact ctcctcatca
    46741 aaaatgggca gatcgaccca gaagcctgtc gttcagttgg cttgtctgca gcggatgtag
    46801 ccctcaaact tcgtagccaa gggattttcc agatgaagca agttaaacga gctgtgcaag
    46861 agcaaaatgg ccaactcatc gtggtccaaa tgggagatga aaatcctaag tatccagttg
    46921 tgactgacgg tgtgatccaa gttgatgttt tggaatcgat tggtcgtagc gaagagtggc
    46981 tgcttgataa cctcagcaaa caaggacatg aaaatgttgc caatatcttt atagctgagt
    47041 atgacaaggg tgctgttaca gtcgtaactt atgaataaga aaaacctggg gtcttgtact
    47101 cttcaaaaat ctcttcaaac tgcgtcaacg tcgccttgcc gtatgtatat gttactgact
    47161 tcgtcagttc tatctgcaac ctcaaagcag tgttttgagc tgacttcgtc agttctatct
    47221 gcaacctcaa agcagtgttt tgagctgact tcgtcagttc tatctacaac ctcaaagcag
    47281 tgctttgagc agcctgaggc tagtttccta gtttgctctt tgattttcat tgagtattgg
    47341 cctcaggttt ccatttgcaa tcagaaaggg attttatgtc cattattcaa aaactttggt
    47401 ggtttttcaa gttagaaaaa cgccgttatt tagtggggat tgtggctttg gtcttggttt
    47461 ccgtactcaa tctcattccc cctatggtca tggggcgggt cattgatgcc atcacatcgg
    47521 ggcaattaac ccagcaggtc ctccttcttg ccctatttta cttgcttctt gcagcctttg
    47581 ggatgtacta tctgcgctat gtttggcgta tgtatatcct tgggacttcc taccgtttgg
    47641 gacagattat gcgctctcgc ttatttgagc atttcacaaa aatgtctcca gctttttatc
    47701 aaacctatcg gacgggggac ctgatggcac atgccaccaa cgatatcaat gccctaacgc
    47761 gtctggcagg tggcggagtc atgtcagcag tggatgcctc aatcacggct ttagtgacct
    47821 tgctgaccat gctctttagc atttcatggc agatgacctt ggttgccatt cttcccctgc
    47881 ctttcatggc ttatgcgacc agtcgtctag ggagaaagac ccacaaggct tttggtgaat
    47941 cacaggctgc cttttctgaa ctcaataaca aggtgcagga gtctgtatca ggtattaaag
    48001 tgaccaagtc tttcgggtat caggcggacg agttgaagtc tttccaggca gttaatgaat
    48061 tgaccttcca aaagaacctc caaaccatga aatacgatag tctctttgac cccatggttc
    48121 tcttatttgt tggttcctcc tatgttttaa cgctcttggt cggctccttg atggttcagg
    48181 aagggcaaat cacggttggg aatctagtta cctttatcag ctacttggat atgctggttt
    48241 ggcctcttat ggccatcggt ttcctcttta atattactca gcgaggcaag gtttcttacc
    48301 agcggattga ggaacttttg tctcaggaat cacctgtaca agacccggag tttcctttag
    48361 atggtattga aaatgggcgc ttgcagtatg ccattgatag ctttgccttt gaaaatgagg
    48421 aaacactgac aaatgttcac tttagtttgg aaaaagggca aactctgggt ttggttggac
    48481 agacaggctc tgggaaaacg tccttgatca aactcctctt gcgtgaatac gatgtggata
    48541 ggggagccat ttacctaaac ggtcacgata ttcgggacta tcgtctgaca gatcttcgca
    48601 gtctcatggg ctatgttcct caggaccagt tcctctttgc gacttcaatt ttagacaata
    48661 ttcgctttgg caattctaag ctgcctcttt cagcggtcga ggaagcgacc aagctagccc
    48721 aagtttacca agacattgta gccatgcctc agggatttga tacgctgatt ggtgaaaagg
    48781 gagtcagtct ttctggaggt caaaagcagc gtctggccat gagtcgggct atgattttag
    48841 accctgatat cttgattttg gatgattcct tatcagccgt ggatgccaag acagagtatg
    48901 cgattatcga caaccttaag gagatgcgaa aggacaagac aaccatcatc acagcccatc
    48961 gcctgagtgc agttgttcat gcagatttga ttttagtcct gcaaaatggt caaatcatcg
    49021 aacgaggcag gcacgaagac ttgctagccc tggatggctg gtatgcccaa acctaccagt
    49081 ctcagcagtt ggaaatgaaa ggagaagaag atgcggaata agaaagaaca atgggatgta
    49141 ttgaagcgcc tgatgtccta tctcaagcct tatggactcc tgaccttttt ggcactcagt
    49201 tttctactag cgacgacggt cattaaaagt gtcattcctc ttgtggcttc ccactttatc
    49261 gaccagtatc tgagtaatct taaccaactg gccattaccg ttttgctggc ctactatggc
    49321 ctttatatcc tacaaactgt agttcagtat gtcggcaatc ttctctttgc gcgggtatcc
    49381 tacagcatcg ttagagatat tcgtcgggat gcctttgcca atatggaaaa gctgggcatg
    49441 tcttattttg acaagacgcc agcaggttct atcgtttctc gtttgaccaa cgataccgag
    49501 acgattagtg atatgttttc tgggatttta tccagcttta tctcagcagt ttttatcttt
    49561 ctgacaaccc tttataccat gttggtgttg gattttcgtt tgacagcttt agtcctgctc
    49621 tttcttccct tgattttcct tttggtcaat atctaccgga aaaagtcagt caagattatc
    49681 gagaaaacca gaagtctctt gtcagatatc aatagtaagc tggcagagaa catcgaggga
    49741 atcagaatta tccaggcctt taatcaagag aaacgcctgc agacggaatt tgatgagatt
    49801 aaccaagaac acttggccta tgccaaccgt tctgtagcct tggatgccct ctttttgcgc
    49861 cctgccatga gtttgctgaa actcttaggt tatgcggtct tgatggccta ctttggctat
    49921 cgtggccttt atctgggaat aacagctgga accatgtatg cctttattca gtacatcaac
    49981 cgtctctttg accccttgat taaggtgacg caaaactttt caaccctgca aacgtctatg
    50041 gtttctgcag gccgagtatt tgccttgatt gacgagagaa cctatgagcc gcttcaaaat
    50101 gatgggcaag ccaaaatcca agaaggcaat attcggtttg aacatgtatg tttctcatat
    50161 gatggcaagc acccgattct ggatgatatt tctttttctg ttaagaaggg tgaaaccatt
    50221 gcctttgtgg gtcatacagg ttctgggaaa tcgtctatta tcaatgtcct catgcgtttt
    50281 tatgaatttc agtcaggcct agttctcttg gatgatgtgg atatcaggaa ctacagtcag
    50341 gaagagttga gaaaaaacat cggtctggtc ttgcaggatc ctttcctcta tcatggaaca
    50401 atcaagtcca atatctccat gtaccaagag atcagtgatg agcaggtaaa ggacgcagct
    50461 acttttgtcg atgcagcttc ctttattcag gatcttcctc agggttatga ttcccctgtt
    50521 tctgagcgtg ggtcgagttt ctctactggg cagcgccagc ttctggcctt tgctagaaca
    50581 gtagccagtc agcctaaaat cctgattttg gatgaagcga cagccaatat tgactctgaa
    50641 acagaaagtt tggttcaagc ttctctggcg aagatgagac agggccgaac aactattgct
    50701 atcgctcacc gcctttctac tattcaagac gccaactgca tctatgtctt ggataaggga
    50761 cgcattatcg agagtggaac ccatgaggaa ctcttggctc tgggaggaac ttatcacaag
    50821 atgtatagtt tgcaggcagg ggccatggcc gatactcttt gaaaatctct ttaaaccatg
    50881 tcagctttat ctgcaatctc aaagctgtac tttgattttc attgagtact agaaggaaat
    50941 ccttcaaatt acagatttct ttcaccgcct tttccatttt gtggtataat gaaaaatgtt
    51001 gacaaatagt ataataaaaa caaaggagaa cagcatgctg aaatgggaag acttgcctgt
    51061 ggaaatgaaa tcaagcgagg ttgagtctta ctaccagctt gtctctaaaa ggaagggttc
    51121 gctgattttc aagcgttgct tggactgggt tttggccttg gtcttactgg ttctaacctc
    51181 tcccatcttt ctcatcttga gcatttggat caagttggat agcaaggggc cagtgattta
    51241 caagcaagag cgtgtgaccc agtacaaccg tcggttcaag atttggaagt tccgtaccat
    51301 ggtgacggat gcggataaaa aaggaagtct ggtgacttct gctaacgata gccgtattac
    51361 caaggttgga aatttcatcc gacgtgtccg tttggacgaa ctgcctcagt tggtcaatgt
    51421 ccttaaaggt gagatgtcct ttgtcggtac acgacctgaa gtgccacgtt atacagagca
    51481 gtatagccct gaaatgatgg caaccttgct cttgcaagca ggaattacct ctccagccag
    51541 catcaactac aaggatgagg acaccatcat cagtcaaatg acggagaaag gtctgtcagt
    51601 tgatcaggcc tatgtggagc atgttcttcc tgaaaagatg cgctataacc tcgcctatct
    51661 ccgagagttt agtttctttg gggacatcaa aatcatgttt caaaccgtgt ttgaggtact
    51721 aaaataaagt agtcataaga aaatgagtac agataaaagg agcaaatcaa tgccaaatta
    51781 caatattcca ttttcaccgc ctgatatcac agaagcagaa attgctgaag tagcggatac
    51841 cctgcgttct ggttggatca caacaggtcc taaaacaaaa gaactggagc gccgcttgtc
    51901 tctttacaca cagacaccta agactgtttg tctcaactct gcgacagccg ctctggagtt
    51961 gattttacgc gttttggaag tgggacctgg tgatgaagtc atcgttccag ccatgaccta
    52021 tacggcttca tgtagtgtca ttacgcacgt gggagcaacc cctgtcatgg tggatatcca
    52081 agcagatacg tttgagatgg actatgacct gcttgagcaa gctatcactg agaaaactaa
    52141 ggtgattatc ccagtagagc tcgcagggat tgtttgcgat tatgaccgtt tgttccaagt
    52201 cgtggagaaa aaacgtgact tctttaccgc ttcaagcaag tggcaaaagg cctttaaccg
    52261 tattgtcatt gtctctgata gtgcccacgc tttgggatct acttataaag gacaaccttc
    52321 tggttctatc gctgacttta cttccttctc attccatgcc gttaagaact ttacaacggc
    52381 agaaggtgga agtgcgactt ggaaagccaa tccagtgatt gatgacgaag agatgtacaa
    52441 ggaattccaa atcctttccc ttcacgggca aactaaggat gctcttgcca agatgcaact
    52501 ggggtcatgg gaatacgata tcgttacacc agcctataag tgcaacatga ccgatatcat
    52561 ggcttcactt ggtttggtac aattggaccg ctatccaagt ttgttgcaac gccgtaagga
    52621 cattgtggac cgctatgata gtggttttgc aggttctcgc atccatcctt tggcacacaa
    52681 gactgaaact gtcgaatctt cacgccacct ctacatcacc cgtgtagaag gagcaagcct
    52741 agaagaacgc aacctcatca tccaagaatt ggctaaagca ggaattgcaa gtaatgttca
    52801 ctacaaaccg cttcctctct tgacagccta taagaatctt ggatttgata tgacgaacta
    52861 tcctaaggcc tatgccttct ttgagaatga aattaccctc cctcttcata ctaaattaag
    52921 cgatgaagaa gtagactata tcattgagac tttcaaaaca gtttctgaaa aagtgctaac
    52981 tttatcaaaa aaatgacaaa ctacagtcaa gcgaaagtga tcctgtcctt aaaaaatcta
    53041 attgagtgta aaaactgttg ttttcaattg ataatagttt acacctgtag ttgaggctcc
    53101 tttctcctca gagagagaat ttttatagga ttttcctttc ttgtgggagt cccgtggttt
    53161 gaaataagat gtgagcaatt tggtgtagca tttagaatcc ttactagaca tcatttagaa
    53221 aatctagtgt cttgttctag ttttcaattc accctatttt ttgaaagacg tgagtttcca
    53281 tgagtgagat tgtggaaact cgcgtctttt tttgttttca gaatatt
//
LOCUS       DAVKLW010000006        52081 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKLW010000006 DAVKLW010000000
VERSION     DAVKLW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.006"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..2724)
                     /locus_tag="VW324_000322"
     CDS             complement(<1..2724)
                     /locus_tag="VW324_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU9726018.1"
                     /translation="MDALTRRQFDRAMFAKNRTLAIRVGDYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKHIKQQGGIEAKIQAQIEATDKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKETKRKAEEALRNAGASSLLAQEAKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELAGASTLLAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEV
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASRIASVQA
                     GSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISFDVTFPVALA
                     NDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNNVFIRFGTNY
                     GFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSLDAGVSRLTE
                     GLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATK
                     DKASKSELTQTAEELSSKIASVQASGRNLFLNSLFKQDISKTGIWTTSTYTA"
     gene            complement(2729..3079)
                     /locus_tag="VW324_000323"
     CDS             complement(2729..3079)
                     /locus_tag="VW324_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726019.1"
                     /translation="MAQLTINGVAVKPPKSFKVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            complement(3088..6693)
                     /locus_tag="VW324_000324"
     CDS             complement(3088..6693)
                     /locus_tag="VW324_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726020.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEAARYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIKSTEAFKRFSNGQSWDQLDFQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSIATIGLGIGVFLAESIANG
                     LGRQKERIIKALVALFDNIGNIAEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKDFEKVVVTNAPKISSIFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWEGSWKPFAEFLSNTFGISIETVADL
                     LGGIILEALKLLADTIKLVADGFTAFSDWCKENKEIISTIASVIGTLATVWQGIKFLS
                     WAEQAGGLAGAFELLSSKVSFIVSGIKNLGLALKALTFDKLVSFGETIYLNALYAKDF
                     VVNSGKTIAQLGKTALELGKSALAWTAHTAKMGLATAAEFAHSVAAGVATAATWAFNA
                     ALAVLTSPITLVIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEF
                     FSGLWTKLQEIFEPIGQWFGEKFQQAWDAIVNIFTPIGSWFGQRWADVTSALANIGAW
                     FTDMFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSWFGNMFTSAYNAVKNAFSS
                     IGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFRSAVNGVLGTIENVVNGFIGMINGVIG
                     MINKIPGVSLGGIGYVSLPRLARGGIVDSPTIAMIGEAGKEAVVPLENTGFIQTLGRV
                     VSSAVVNAMAGISPQGGFSGDGDIVIQIAGHEFGRVAIQEINKEHERAGQTLLKI"
     gene            complement(6680..7030)
                     /locus_tag="VW324_000325"
     CDS             complement(6680..7030)
                     /locus_tag="VW324_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726021.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKVFEFWEYAPELFVEEQQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEVNGNS"
     gene            complement(7069..7449)
                     /locus_tag="VW324_000326"
     CDS             complement(7069..7449)
                     /locus_tag="VW324_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726022.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVQ"
     gene            complement(7454..7867)
                     /locus_tag="VW324_000327"
     CDS             complement(7454..7867)
                     /locus_tag="VW324_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU9726023.1"
                     /translation="MLANGITLSYSKTKGSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYELGIGDVGELEYKFSYNNSSATAPYRVLRKAADDKEKLYFEQAYPDGTKVTFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSELEFADGVGG"
     gene            complement(7873..8241)
                     /locus_tag="VW324_000328"
     CDS             complement(7873..8241)
                     /locus_tag="VW324_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726024.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDKEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            complement(8238..8753)
                     /locus_tag="VW324_000329"
     CDS             complement(8238..8753)
                     /locus_tag="VW324_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEU9726025.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNAAIKVVQADAR
                     RLVPVNDGELITSIKTRAKMDGDRAIGEVYTNLKYAPYVEFGTGPKGQASHSGISPEV
                     SVTYKSHPWYVHEDQINVGSYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIQ"
     gene            complement(8728..9066)
                     /locus_tag="VW324_000330"
     CDS             complement(8728..9066)
                     /locus_tag="VW324_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726026.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            complement(9047..9358)
                     /locus_tag="VW324_000331"
     CDS             complement(9047..9358)
                     /locus_tag="VW324_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU9726027.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            complement(9360..9548)
                     /locus_tag="VW324_000332"
     CDS             complement(9360..9548)
                     /locus_tag="VW324_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726028.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVT
                     ISDVLEKILPEPEFLEED"
     gene            complement(9538..9720)
                     /locus_tag="VW324_000333"
     CDS             complement(9538..9720)
                     /locus_tag="VW324_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEU9726029.1"
                     /translation="MSLLLRRHYIQEEQVNQYSDLENKTLEELKDLAKEAGVAGAYKL
                     TKAEIVEVLEELKSEI"
     gene            complement(9732..10577)
                     /locus_tag="VW324_000334"
     CDS             complement(9732..10577)
                     /locus_tag="VW324_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEU9726030.1"
                     /translation="MTAGQTKLATMVNPEVMADMVAAKLPKLIKFTPLAYVETKLEGQ
                     PGSTLTVPAWEYAGDATEIEEGQAITPDQLTTKKTTMTIKKAGKGYEITDESLLSGLG
                     DPLGQATYQLGLAIANKIDNDLVAVAKTATQHITETPTTLEAIDKALDIFEDEEDAQY
                     VAIINPKDATKLKTAVAKEWIKGSELGANMVVSGTFGEVDGVQIVRSKKVDEGKGFLV
                     KVSPSQTQTDDANKYGAFVILLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGGA"
     gene            complement(10584..11168)
                     /locus_tag="VW324_000335"
     CDS             complement(10584..11168)
                     /locus_tag="VW324_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEU9726031.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            complement(11385..11636)
                     /locus_tag="VW324_000336"
     CDS             complement(11385..11636)
                     /locus_tag="VW324_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726032.1"
                     /translation="MLEKAKQLASQEFSRLSGREIKAEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGNYAEITHNGDKKETYVDVYAKVSNRAIKD"
     gene            complement(11638..11883)
                     /locus_tag="VW324_000337"
     CDS             complement(11638..11883)
                     /locus_tag="VW324_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726033.1"
                     /translation="MKYRKKPVVVEAVQWNGNNHKEVIDFAENKIWFDALGNIWIATL
                     EGDMVAKKGDYIIKGVQGEYYPCKLDIFAETYEKTEE"
     gene            complement(11926..12339)
                     /locus_tag="VW324_000338"
     CDS             complement(11926..12339)
                     /locus_tag="VW324_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9726034.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIHYLST"
     gene            complement(12336..12545)
                     /locus_tag="VW324_000339"
     CDS             complement(12336..12545)
                     /locus_tag="VW324_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726035.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            complement(12547..14184)
                     /locus_tag="VW324_000340"
     CDS             complement(12547..14184)
                     /locus_tag="VW324_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEU9726036.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEI
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPENEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLISLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKITTDSQAENISLSKATSKINDALRYTTIFDPDTFAKEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNDSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            complement(14093..15562)
                     /locus_tag="VW324_000341"
     CDS             complement(14093..15562)
                     /locus_tag="VW324_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU9726037.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSHLKKLKSYYM
                     GEHAILKHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            complement(15574..16872)
                     /locus_tag="VW324_000342"
     CDS             complement(15574..16872)
                     /locus_tag="VW324_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEU9726038.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVVRLLKYSWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAELRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            complement(16850..17290)
                     /locus_tag="VW324_000343"
     CDS             complement(16850..17290)
                     /locus_tag="VW324_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEU9726039.1"
                     /translation="MSGLRIKQKRFADEYIISGNATEAYKKAGYRVSSDRVAGVEGHK
                     LLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQTLISIGELGQTITD
                     IDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDIPD"
     gene            complement(17595..17677)
                     /locus_tag="VW324_000344"
     tRNA            complement(17595..17677)
                     /locus_tag="VW324_000344"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17644..17646),aa:Tyr,seq:gta)
     gene            complement(17758..18162)
                     /locus_tag="VW324_000345"
     CDS             complement(17758..18162)
                     /locus_tag="VW324_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEU9726040.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            complement(18235..18606)
                     /locus_tag="VW324_000346"
     CDS             complement(18235..18606)
                     /locus_tag="VW324_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726041.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            complement(18603..19040)
                     /locus_tag="VW324_000347"
     CDS             complement(18603..19040)
                     /locus_tag="VW324_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726042.1"
                     /translation="MTPRYRAWIKTEKRMFFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIG
                     IVKQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            complement(19052..19474)
                     /locus_tag="VW324_000348"
     CDS             complement(19052..19474)
                     /locus_tag="VW324_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726043.1"
                     /translation="MTETIKLPDYYEPDWKNARYGSLEELKELLLFKRIVKWDKDFLL
                     LEDGTKVTIEMSESDCCASAGGEFQDVSLDAVITNIEIGEPEEIPDHWGTGYKNKVTI
                     FHNQNPVAIANCEAEHNGYYYSVCSLVIGDIHFPVVNA"
     gene            complement(19471..19731)
                     /locus_tag="VW324_000349"
     CDS             complement(19471..19731)
                     /locus_tag="VW324_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEU9726044.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYDKVKVGDEVLL"
     gene            complement(19889..20377)
                     /locus_tag="VW324_000350"
     CDS             complement(19889..20377)
                     /locus_tag="VW324_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU9726045.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPEWFYENNIEKFCLAWLDGYEVEKE
                     KRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEDANFGWVFDCPGIEIEE
                     VE"
     gene            complement(20379..20696)
                     /locus_tag="VW324_000351"
     CDS             complement(20379..20696)
                     /locus_tag="VW324_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726046.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWIGYSPSMAQVVIAVYLIY
                     GGN"
     gene            complement(20721..20903)
                     /locus_tag="VW324_000352"
     CDS             complement(20721..20903)
                     /locus_tag="VW324_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726047.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            complement(20919..21350)
                     /locus_tag="VW324_000353"
     CDS             complement(20919..21350)
                     /locus_tag="VW324_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEU9726048.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRARLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            complement(21347..21676)
                     /locus_tag="VW324_000354"
     CDS             complement(21347..21676)
                     /locus_tag="VW324_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726049.1"
                     /translation="MASKINATERIAIIIDKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            complement(21690..21899)
                     /locus_tag="VW324_000355"
     CDS             complement(21690..21899)
                     /locus_tag="VW324_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726050.1"
                     /translation="MEWTDWVDWKPETKTDIKTKIENDGYTFPHYDKKNNGVKYVIST
                     LDIKRDCLRLGVPFEDVYPLQTTLF"
     gene            complement(21901..22596)
                     /locus_tag="VW324_000356"
     CDS             complement(21901..22596)
                     /locus_tag="VW324_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9726051.1"
                     /translation="MEIDKIIKKDVLEFMETIPDNKIDLIVTDPPYLINYKTNWRKEK
                     HKFSNVIKNDNNPELIKEYIKECYRILKDDTAIYIFCSFDKVDFFKKEIEKYFSVKNI
                     IIWRKNNHTAGDLEAQFGKQYEMIILANKGRKKFNGERLTDVWDFKRVSSDKLLHQNQ
                     KPIELIKRCIVKHSDVGDTVFDGFMGSGTTALAALETDRHFIGTEIDEYYFGIAEERI
                     KNHNAQLSLFDEV"
     gene            complement(22609..23025)
                     /gene="ssb"
                     /locus_tag="VW324_000357"
     CDS             complement(22609..23025)
                     /gene="ssb"
                     /locus_tag="VW324_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9726052.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGLTGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            complement(23120..23455)
                     /locus_tag="VW324_000358"
     CDS             complement(23120..23455)
                     /locus_tag="VW324_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEU9726053.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            complement(23448..23771)
                     /locus_tag="VW324_000359"
     CDS             complement(23448..23771)
                     /locus_tag="VW324_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726054.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFNVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            complement(23939..24979)
                     /locus_tag="VW324_000360"
     CDS             complement(23939..24979)
                     /locus_tag="VW324_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEU9726055.1"
                     /translation="MQELQVKVTQAQVEIIDREKFEQNINEVVAKYQNYTVTAATIKD
                     DKQTLADLRKLDKQVSDERIRNKKVLSEPADEFDKYVKNAIQPLKDIITKIAGDVKEF
                     EEHQKAVRIDTVKGYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTLKKATMKSL
                     DDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFEQIKTDYEFE
                     KQKEELRQDLERAERSNQELLAAQQTKQQEQAPKSTETPNFDPETGKILDGGQILQNE
                     PNALRGAENDLKRYAQKMTLEVYFVDTAEKDCFKAGLSKLGFDFKKNYQVSGYQRIDP
                     LTQAELNEQCGW"
     gene            complement(24989..25753)
                     /gene="bet"
                     /locus_tag="VW324_000361"
     CDS             complement(24989..25753)
                     /gene="bet"
                     /locus_tag="VW324_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEU9726056.1"
                     /translation="MTNNQLATQIKRDITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYQNNSGTEFSLIVSKEAFMKRAERCEGYDGFEAGITVM
                     RNGEMIEIEGSLKLPEDILIGGWAVVYRKDRSHRYKVTVDFNEYVKTDRNGNPRSTWK
                     SMPATMIRKTALVQTLREAFPDELGNMYTDIDGGDTFDAIKDVTPQESREDVVARKMA
                     QIDQFNKEQEANHADPEPTQNEDPIQGELLDGELEY"
     gene            complement(25765..25995)
                     /locus_tag="VW324_000362"
     CDS             complement(25765..25995)
                     /locus_tag="VW324_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726057.1"
                     /translation="MTQMTNKGKSFIRAEISEKQKEYIRLLAELRGVTTQELLGQVVE
                     RFIDRNLQLIQDYKNELDDLNSKSRRRIDMNT"
     gene            complement(26115..26258)
                     /locus_tag="VW324_000363"
     CDS             complement(26115..26258)
                     /locus_tag="VW324_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726058.1"
                     /translation="MTKIEIENRVWLLANHEEKNELLDLGLTSKARYVKRVLELGKVY
                     AHV"
     gene            complement(26245..26505)
                     /locus_tag="VW324_000364"
     CDS             complement(26245..26505)
                     /locus_tag="VW324_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726059.1"
                     /translation="MFKALKTIKKIKQLQKEMHDVSLAFLALQDVGLMPETERSKAKA
                     QTMHDVSHMLKDVLGGKSVDEAMKRLNSEVKIEEVEQEDDKD"
     gene            complement(26518..26772)
                     /locus_tag="VW324_000365"
     CDS             complement(26518..26772)
                     /locus_tag="VW324_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726060.1"
                     /translation="MYIISIYVKNTETGNEDFSLIGRDFLPTGHQDYIARVFETKEEA
                     IDYLKSISYIASGVHGNDWVYQNEKLPEIESRCRIWKVGE"
     gene            complement(26773..26994)
                     /locus_tag="VW324_000366"
     CDS             complement(26773..26994)
                     /locus_tag="VW324_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726061.1"
                     /translation="MKLLTKLKLRLEVVLKAVNLDWREVAVELMTDLFEERKRRFAFE
                     QENYDLKQELAAYKYKENFDIKARLQGEV"
     gene            complement(26994..27155)
                     /locus_tag="VW324_000367"
     CDS             complement(26994..27155)
                     /locus_tag="VW324_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726062.1"
                     /translation="MKRKKEQWKPRIVNIMADGSQVDDLTGYVIPAGHSYYDIILGMN
                     KQSNEEGVA"
     gene            complement(27220..27363)
                     /locus_tag="VW324_000368"
     CDS             complement(27220..27363)
                     /locus_tag="VW324_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726063.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            27481..27705
                     /locus_tag="VW324_000369"
     CDS             27481..27705
                     /locus_tag="VW324_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015990842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726064.1"
                     /translation="MSKLSRKPNHHVKKLTWSDLDNILISAFTESATDHPSAVIRLSD
                     YEMSKSEIIEEAIAQGYTVIDNSDDFLEFE"
     gene            complement(28016..28282)
                     /locus_tag="VW324_000370"
     CDS             complement(28016..28282)
                     /locus_tag="VW324_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9726065.1"
                     /translation="MMVVLANHPNWQVYPDEIAKRKGVSRDTVDSYFKILEKNGYLRI
                     VKKGMGRGKGVRVFRFFSDVKISDFQFEIMKQRLNESISKLSTG"
     gene            complement(28447..28635)
                     /locus_tag="VW324_000371"
     CDS             complement(28447..28635)
                     /locus_tag="VW324_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9726066.1"
                     /translation="MQGERLRKWREKEKMSQEELAEKSNVSRTTIHLIESGQSSTVKI
                     RTLQKLAVVFNKQVKDFF"
     gene            complement(28688..28849)
                     /locus_tag="VW324_000372"
     CDS             complement(28688..28849)
                     /locus_tag="VW324_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726067.1"
                     /translation="MRPIGYRLNVEVSGIEELKEACKEVSKKAEELQEAIDRLSIIEF
                     ELKAKPVND"
     gene            28896..29702
                     /locus_tag="VW324_000373"
     CDS             28896..29702
                     /locus_tag="VW324_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEU9726068.1"
                     /translation="MLSIDTQFVDTISKILSDYVSHSEITRMGEVLGYPQNDQNSGLN
                     KHHRVHNIMSDILNKTQDKSNIKLVIEYICNPLRYIDTVSDFENLRLKLNVVLSLKGL
                     TISDDGHVVITTASQTLVEAKKRFESLEHMLRTLNVHPNVLKFCTQELLQENYFHAVF
                     EASKGIFHRIRLLTGSSLDSASLIDQCFKTKEPIMIINGNKLQTLDEQSEYKGLKNLL
                     LTIAHLYRNSKAHKLKYYNPDSVHDALTALTLMSLAHNLLDNCTNTRRLD"
     gene            complement(29637..29930)
                     /locus_tag="VW324_000374"
     CDS             complement(29637..29930)
                     /locus_tag="VW324_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726069.1"
                     /translation="MEKLAEVTQEETEKILTYDEIVLLKPITHLHKSIFRQTEYLSEQ
                     LTERLHHLEDYNSPIDEETIRLAEVTIEFYNLLIKSPSISTVVKEIVSEGHKS"
     gene            30173..30622
                     /locus_tag="VW324_000375"
     CDS             30173..30622
                     /locus_tag="VW324_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726070.1"
                     /translation="MNNFSFIETLAIAAIPAFVSGMWSYIAAKGNSKHEIDKINIAHS
                     QELENVENQFKQDMEKMQKQHSQELYSLQQTHELRLLELEKVSQLDTQTDQGLKINDL
                     IFKAISGEISADVAIKNISTLSHFANKQQPSDLQKQFMKKLSKKNHK"
     gene            complement(30613..30780)
                     /locus_tag="VW324_000376"
     CDS             complement(30613..30780)
                     /locus_tag="VW324_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726071.1"
                     /translation="MNESFLTILGISMIASFITNLIAYLAGKHHLKKKIKNHKMWFDS
                     EIERIKKKYHL"
     gene            complement(30855..31130)
                     /locus_tag="VW324_000377"
     CDS             complement(30855..31130)
                     /locus_tag="VW324_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726072.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLETFSTNCRQF
                     TFGWTELADEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKMNVA"
     gene            31285..32073
                     /locus_tag="VW324_000378"
     CDS             31285..32073
                     /locus_tag="VW324_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9726073.1"
                     /translation="MKLGELLKSYRTEHKLSMDAFCELSDLTKGYISMLEKNEHPKSK
                     KPIVPSYDTIEKIAKGMQISTEDLIDMLDDDQEIQINATPALLSKSPIQTIYDELEPP
                     RQGKVLNYAKRQLDEQENEEETKINEVSENIIRLDDYRQTTYRRVTGVVSAGSGSIQD
                     DDLDMEVSFYEDEIPDDYDAIAYVVGNSMEPKIKNGDYLFIKNTQQVDYNTIGIFQVD
                     GANYVKKLRQGYLESLNPDYEDIHLDESNDIRTIGEVVSVYREK"
     gene            32075..32275
                     /locus_tag="VW324_000379"
     CDS             32075..32275
                     /locus_tag="VW324_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726074.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            32457..33902
                     /locus_tag="VW324_000380"
     CDS             32457..33902
                     /locus_tag="VW324_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9726075.1"
                     /translation="MITTNKVAIYVRVSTTNQVEEGYSIDEQKDKLSSYCDIKDWNVY
                     KVYTDGGFSGSNTDRPALESLIKDAKKRKFDTVLVYKLDRLSRSQKDTLHLIEDVFIK
                     NGIEFLSLQENFDTSTPFGKAMIGLLSVFAQLEREQIKERMQLGKLGRAKSGKSMMWA
                     KTSYGYDYHKETGTVTINPAQALTIKFIFESYLRGRSITKLRDDLNEKYPKHVPWSYR
                     AVRTILDNPVYCGFNQYKGEIYPGNHEPIISKEEYDKTQSELKIRQRTAAENVNPRPF
                     QAKYILSGIAQCGYCGAPLKIMLGVKRKDGSRLKKYECHQRHPRTLRGVTTYNDNKKC
                     DSGFYYKDKLEASVLKEISKLQDDADYLDKIFSGDNAETIDRESYKKQIEELSKKLSR
                     LNDLYIDDRITLEELQSKSAEFISMRATLETELENDPALRKNKRKADMRKLLNAEKVF
                     SMDYESQKVLVRRLINKVKVTAEDIVINWKI"
     gene            complement(33920..33982)
                     /locus_tag="VW324_000381"
                     /pseudo
     CDS             complement(33920..33982)
                     /locus_tag="VW324_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
     gene            complement(33984..35000)
                     /locus_tag="VW324_000382"
     CDS             complement(33984..35000)
                     /locus_tag="VW324_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9726076.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(34948..35889)
                     /locus_tag="VW324_000383"
     CDS             complement(34948..35889)
                     /locus_tag="VW324_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9726077.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(35965..36330)
                     /locus_tag="VW324_000384"
     CDS             complement(35965..36330)
                     /locus_tag="VW324_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9726078.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(36481..37539)
                     /locus_tag="VW324_000385"
     CDS             complement(36481..37539)
                     /locus_tag="VW324_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9726079.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(37702..38853)
                     /gene="nagA"
                     /locus_tag="VW324_000386"
     CDS             complement(37702..38853)
                     /gene="nagA"
                     /locus_tag="VW324_000386"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9726080.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(39006..40823)
                     /locus_tag="VW324_000387"
     CDS             complement(39006..40823)
                     /locus_tag="VW324_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9726081.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(40921..42063)
                     /gene="tgt"
                     /locus_tag="VW324_000388"
     CDS             complement(40921..42063)
                     /gene="tgt"
                     /locus_tag="VW324_000388"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9726082.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            42193..43050
                     /locus_tag="VW324_000389"
     CDS             42193..43050
                     /locus_tag="VW324_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9726083.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(43078..43722)
                     /gene="pcp"
                     /locus_tag="VW324_000390"
     CDS             complement(43078..43722)
                     /gene="pcp"
                     /locus_tag="VW324_000390"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9726084.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(43829..44188)
                     /locus_tag="VW324_000391"
     CDS             complement(43829..44188)
                     /locus_tag="VW324_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9726085.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            complement(44199..44624)
                     /locus_tag="VW324_000392"
     CDS             complement(44199..44624)
                     /locus_tag="VW324_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9726086.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(44947..46059)
                     /locus_tag="VW324_000393"
     CDS             complement(44947..46059)
                     /locus_tag="VW324_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9726087.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(46225..46845)
                     /locus_tag="VW324_000394"
     CDS             complement(46225..46845)
                     /locus_tag="VW324_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9726088.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(46849..48129)
                     /locus_tag="VW324_000395"
     CDS             complement(46849..48129)
                     /locus_tag="VW324_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9726089.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(48190..49674)
                     /locus_tag="VW324_000396"
     CDS             complement(48190..49674)
                     /locus_tag="VW324_000396"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9726090.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(49750..51027)
                     /locus_tag="VW324_000397"
     CDS             complement(49750..51027)
                     /locus_tag="VW324_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9726091.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(51134..51217)
                     /locus_tag="VW324_000398"
     tRNA            complement(51134..51217)
                     /locus_tag="VW324_000398"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51181..51183),aa:Leu,seq:caa)
     gene            complement(51227..51298)
                     /locus_tag="VW324_000399"
     tRNA            complement(51227..51298)
                     /locus_tag="VW324_000399"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51264..51266),aa:Gln,seq:ttg)
     gene            complement(51312..51384)
                     /locus_tag="VW324_000400"
     tRNA            complement(51312..51384)
                     /locus_tag="VW324_000400"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51349..51351),aa:His,seq:gtg)
     gene            complement(51399..51469)
                     /locus_tag="VW324_000401"
     tRNA            complement(51399..51469)
                     /locus_tag="VW324_000401"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51435..51437),aa:Trp,seq:cca)
     gene            complement(51474..51554)
                     /locus_tag="VW324_000402"
     tRNA            complement(51474..51554)
                     /locus_tag="VW324_000402"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51518..51520),aa:Tyr,seq:gta)
     gene            complement(51567..51639)
                     /locus_tag="VW324_000403"
     tRNA            complement(51567..51639)
                     /locus_tag="VW324_000403"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51604..51606),aa:Phe,seq:gaa)
     gene            complement(51643..51716)
                     /locus_tag="VW324_000404"
     tRNA            complement(51643..51716)
                     /locus_tag="VW324_000404"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51680..51682),aa:Met,seq:cat)
     gene            complement(51727..51816)
                     /locus_tag="VW324_000405"
     tRNA            complement(51727..51816)
                     /locus_tag="VW324_000405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51778..51780),aa:Ser,seq:tga)
     gene            complement(51899..51972)
                     /locus_tag="VW324_000406"
     tRNA            complement(51899..51972)
                     /locus_tag="VW324_000406"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51936..51938),aa:Ile,seq:gat)
     gene            complement(52007..52077)
                     /locus_tag="VW324_000407"
     tRNA            complement(52007..52077)
                     /locus_tag="VW324_000407"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52043..52045),aa:Gly,seq:tcc)
BASE COUNT        14694 a        11987 c         9193 g        16207 t
ORIGIN      
        1 agccgtatat gtactcgttg tccaaattcc tgtttttgaa atatcctgct tgaatagtga
       61 gttcaagaat agatttcgac cggatgcctg cacactcgct atcttactag agagctcctc
      121 agctgtctgc gtgagttctg acttgctggc tttatccttg gttgcattca ggatttcctg
      181 acggatagag ccagctcgca cctcaaattc agcctgactc aacttctgat ttagtttgtt
      241 ctgcgtgtct gtctcaaggc tcttcacaga ttgccggata ttttcagcag tcacattgag
      301 tgagctgata tcggctttag ttctaagccc ttcagtcaga cggcttacac cagcgtcgag
      361 tgaatcagca cgctgcttaa agttggattc gactactgag acacggtctt cttggtcttc
      421 atacgctggt tgataggctg gaaaataatt accaaccgat aacatagcgt tctcaatgac
      481 gacctgcaga ccagcaggaa atccataatt agtaccaaaa cgaatgaaca cattattagt
      541 ctgatagtcc tcagaagaac tagacaagtc aatcgtaaac tcaaaatgtt ggcgttcgac
      601 agttccacct ttaaaaatta agtttctgta ggcataccat ggatgagcac taaaatgcac
      661 catagcaggc atgtcatttg ctagggcgac agggaaagtc acatcaaaag atatgcgaac
      721 ataatcacgc ttgaacctgt cactgttctt ccagaaatca ggaactatga atgttcgata
      781 gtcgtatacc gcttgacctc ctgttgtgaa cgttcttgaa cgtgaattcc tgaagtaatt
      841 ccgtgaacta cctgcctgca cactcgcaat ccgactagcc agctcctcag ctgtttgcgt
      901 gagttctgac ttgctggctt taccattggc caagttggtc agttctgaca gtctacgagt
      961 cgtcgtctcc tcatacgtcg cttgcgctga cttcacacca gccagttcat tcttagtccg
     1021 actaagtact tcaacttgct tggcaatctc agcttcagcc tgtgcttgct tcggtcgaat
     1081 atcgtttgcg atagttcgtt ttagaacatc caagtcacct gacaaagccg tctgagcgct
     1141 cgtagtctgc gacttaaacg cttcaagtct agcaacagaa tccagctcaa tccgcttcgc
     1201 ttcctgtgca agcagggtac ttgcgccagc cagttctttt ttggtctgaa caagtgcttc
     1261 aacttgcttg gcaatctcag cttcaacctg tgcttgcttc ggtcgaatat cattcacgat
     1321 agtccgtttc agagcgtcca agtcacctga cagagccgtt tgtgcgctcg tagtctgcga
     1381 cttaaacgct tcaagtctag cgacagaatc cagcccaatc cgcttcgctt cctgagcgag
     1441 taagctgctt gcgccagcat ttcgcaaagc ttcctcagcc ttgcgcttag tttctttcaa
     1501 tggcccgttg tcaaagctat taaatcgctg attgatagtg tcagacagtt ctctcttgac
     1561 ttcttcagct ctggctttgg ccagttctac ttgatcgtta aagtcttttt tgattttgtc
     1621 gaccttttgg tcaaaatctt tatctgttgc ttcaatctgc gcttggattt tcgcttcaat
     1681 gccaccttgt tgcttaatat gcttggtaat cgtaccctcg taagaatact gagtatcgtt
     1741 tccagcctta ctatctgcgc tgatacgccc tctcagacca cctttaaaag taaagctctg
     1801 acttaacacg ggaactttaa aagtctcttt cttgttggtc tgaatggtta cccactgccc
     1861 aacctcaagt aacaaatgtc cttggtagtt gagattatac ggatagtaag ttaggttttt
     1921 cagtttgtaa tacaggtcat ttaaagcgct ctgggtcatg aagacattgt ccagttccaa
     1981 agaccgacct gtcttcatac cgaccgtcag agacttcttg tccgtcttac aagtgatacc
     2041 agctatctga tactcaatct cactcttggt caagccatgc aagaagtaac tgtcagcgtt
     2101 gatcgtgata ttggactcag tcaaatcacg gatttccatc ttgccttctc tgttgaagaa
     2161 acaagacatc ccaatcatct gagtcatagc gctcagcata tccctaaagg aaagtttctt
     2221 gccctcagga acttgctcaa tatgataacg catcgcgctg attccgaaat agtcattcgc
     2281 taactcaatg cctgttttca ggcagatttc ctgaataacc tctcgtactt cagctgggaa
     2341 atgcaaatcc gtcacgtact cacgattgag cttaaacata ccgtccataa gttcaagtgt
     2401 ggttgtgttg cggtttcggt caatctcaat atcgttgatg aagtattccc ccatcttgac
     2461 ccactggtag gtatccccaa ccagtagacc aatctcaggg tgcagggtat ccagcttatt
     2521 gaacgtggta atgatactgg taaaggtaat tttaccgcta ccagcgcagg ttccaccagg
     2581 cttataagta tcgcccttga tgtagccata ctcaaaacta gcctctttga tatcccgtga
     2641 agcataatct ccaacacgga tagccagcgt cctgtttttg gcaaacatgg ctctgtcaaa
     2701 ttgtcgtcta gttaaagcgt ccattttctt acctctctac cagattaaat ttagcgccag
     2761 accaaggttt aaacttctca gtaaaggtat agctaggagc tgtcctatca ccgacataga
     2821 aagtctttgt gacttggcca tccatggggt ctggataaga cacctcaaaa aattcagatg
     2881 atacagcatg taaaagctga cttatttctc cctgagtcat cataccccat tcacagtcta
     2941 gtttgcgttt ggtcgtgata cggtcacgca ccatgtcgcc attggcatta cgccctgtct
     3001 ctccatcgat atcttgaata ccgactttaa aagatttggg aggcttcaca gccaccccat
     3061 tgattgtcaa ttgtgccatt taacctccta aatcttgagc aaggtttgac ctgctcgttc
     3121 atgttccttg ttgatttctt ggatagctac ccgtccgaac tcatggcctg cgatttgaat
     3181 aacgatgtcg ccgtcaccag aaaaaccacc ttgtggacta ataccagcca tggcatttac
     3241 taccgcactg ctgacaactc gcccaagggt ttggataaag cctgtatttt caagtggtac
     3301 gaccgcttct ttaccagctt caccaatcat ggcgattgtt gggctatcga cgataccacc
     3361 acgggcaaga cgaggtaggc tcacatatcc aataccacca agggatacgc caggtatttt
     3421 gttaatcatg ccaataacac cattgatcat gccgatgaag ccattgacta cattttcaat
     3481 agtcccaaga acaccattga ctgcacttct gaaagcccca cctacagcgc taccaacctt
     3541 ttgtccagca ttgacaaata tgctttgaac cgttgaccat acaccgctga agaagccacc
     3601 aattgaacta aacgcgttct tgactgcatt ataagcacta gtaaacatat tcccaaacca
     3661 agaagataca ttcgcaagaa cacttgtaac atctgcccat ctctcgccaa accataaacc
     3721 tagtttgcta aagatgtttg ttagaccagt ccatgctttt tggaacatgt cagtaaacca
     3781 tgccccgata ttagccaagg cactagtcac atccgcccaa cgttgtccaa accatgagcc
     3841 gattggtgtg aagatattaa cgatagcatc ccatgcttgc tggaacttct cgccaaacca
     3901 ttgacctatt ggctcaaaga tttcttgtag ttttgtccat agaccactga aaaattcgcc
     3961 aatagctcga caaataccac taataaaatc acatagtcct tgccatgcag ttttagcaaa
     4021 ctcaacaaca gtgtcccagt tttggtagag caagacacca atagcaatta aggctgcaat
     4081 agcagcaata actaaggtta ttggacttgt caaaactgct aacgctgcat taaaagccca
     4141 tgttgcagct gtagcgactc ctgctgccac agaatgtgca aattccgccg cggttgctaa
     4201 tcccattttc gctgtatgag cagtccatgc tagagctgat ttaccaagtt ctaaagcagt
     4261 tttccctagc tgtgcaattg ttttacctga attgacaaca aaatcttttg catacaacgc
     4321 attcaaatag atggtttctc cgaagctgac caatttatca aatgtcaaag ctttcaaagc
     4381 tagtccaaga tttttaattc cgctaacaat aaaggaaacc ttactactta ataattcgaa
     4441 tgcccctgca agtcctccag cttgttcagc ccaagacaag aacttaattc cttgccacac
     4501 ggttgcaagc gtaccaatca cactagcgat tgtggagata atctctttat tttctttaca
     4561 ccaatctgaa aaagcagtaa aaccatcggc tactagcttg attgtatcag ctagtaactt
     4621 caatgcctct agtatgatac cgcctagtaa atcagcgacg gtttcaatac ttatgccgaa
     4681 tgtgttagac aagaactctg caaaaggctt ccaacttcct tcccaaagta tttgaataat
     4741 gtcaattagc ccattaaaag cattagcaat agagttaata gcaggggcta catgttcatc
     4801 ataaacacga cttaagccat cgccaaattt gttaacagac ctttcaatgc tctcaaatac
     4861 aggcgcaaca gtatctaata aactttggaa gattgatgaa attttaggag cgtttgtcac
     4921 aacgactttt tcaaaatctt taaacaaact tcctgctaat ttactaccaa cttcaacaat
     4981 ggtagatgtc aaactcaaca gagttgacac aatagcgcta ccgatacgaa ccgcaccagt
     5041 tgaggtaatg acgtcgtaga aagcactaga aaagtcctga gcgatgtttc ctactgcctc
     5101 ggcaatgtta ccaatattat caaacaaagc gactagcgcc ttgataatgc gttctttttg
     5161 ccttccaagg ccattggcaa tactttcggc aaggaaaaca ccgataccta gcccgatagt
     5221 agctattgag cctgtcactt gccctaaagc ataagcaatt ttctcagcca ttcggttaaa
     5281 ggcattcaca acccttgggt cagtggcgat ttctcccatt gtcttagcta tttggtctaa
     5341 ggcagtctta atgcgtttta taccttctgg tctaaatgct gcatcaaaac ctttcttgaa
     5401 gaggtcaaac aaccctttga gcttatctcc aagaccatca aaaatgctct tgaatttgtt
     5461 gtccatgtcg gtcaactcga cttctggcaa gatgtctttg aaaggtccgc caccgcctcc
     5521 ctttccttta ccacctttgc caccgcctcc agacccgcct gcgtcgtcat cttttggttt
     5581 ttgcaagatg ttaatctcat caaatcccaa aagacctagc aactctttag cggccttctt
     5641 agcgtttttg gcggagtctc caagattgtc agcaagtcct cctgctgaat ctccagcgtc
     5701 gtctactgcg tcagcaaggt ctcctgctcc gcctgcagca tccttcatgg cgttacccat
     5761 gtctccaact gctccaccaa caccatcttt cactgttgct ttcttgttga acatcaaagc
     5821 gataaactca gcgagtttag cagtaacgtt ctttaagacc atagcaaaag agttcaagac
     5881 aggcataatg gcattgataa tcggtaacat agagttacca aggttcaatg ctgcgtcctt
     5941 cattagcgac ttaaacagac tgatacgacc atttacagaa ttagacaagg tatctccata
     6001 cttggctgta gcttgctcca ggatagccat aagacggatt tgttgctggg tttgaaagtc
     6061 gagttggtcc caactttgac catttgagaa acgtttaaaa gcttcagtgg acttaatcat
     6121 agccacattg acgttgattc ctaggtcctc aattgcttcg gtgttcccta gtagacctga
     6181 gcgaatccgc tccataacgt ctgtaatcgt gcgccctgaa ccttcagcaa ccactgccga
     6241 tgtctgcaac atcttagcag tataggcgct tagcttattg gtatctttga taaatccaga
     6301 aaacaggttg gagtaaaccg caccgtacct ggccgcttca cctacaccca tattcattgc
     6361 atttgcgtta tcgttaaccc attttaagaa agattgcgaa ctctcgccca tctgacgctt
     6421 gatttggttc atagacgctg acacttcaag agctgtctgc gttgaataca tcccaacatc
     6481 aagtaatttc ttaccaagga ttgcaaaacc agcgaactta gccagcttgc caaacgcact
     6541 accgatagag ttcgactgtt cacgaacttt ggcagtggca tttttcactt ggtcagatgt
     6601 tcctttgacc tgattctcga cttctttcat cttcttcctg aaaggcgcta tctcagcgtc
     6661 aatcatgacc ttcaattcat caagagttgc cattcacttc ctcctttctt ttgcgattgt
     6721 gtctctctgc aaaatcacgc atccgttcct tatgcaacaa aagcgcttgt ctctgtcgtt
     6781 cctgttctac tgcttgctgt tcttctacaa acaactcagg cgcatattcc cagaactcaa
     6841 aaaccttggc atccttggac aataacaaag aaacgtggtt ggatatcatc tgcgaaagtc
     6901 tgtatgagtc aataatcttc tctttacgct cttggatttt gacacggttg tagctttcta
     6961 tcatttccct gatttcaagc accgtcaaat cccaaaaatc aagaggctta cctccgatgt
     7021 ccaaaaacat tgggtaaagt ctctcaataa tctgcgttac cgttaagatt actggactac
     7081 tgtcattttc ttcttggaag ttttcttgtc cttgcttcct cgtggagtaa aacccgatac
     7141 ttcaaagagt ggcattaaca cctctgtcat gaatgttgtt tggtctccac cattgtccac
     7201 gtattcatcg tataaatcat agacatcctc aagagaatac ccatgttcat actgctgcaa
     7261 ggcgccgtga actaacaaca acataacttt caaaggtggc aaagtgaact cttcgcctgc
     7321 ttcaggcatg aaaatcttca gcaagttcat gccgattttt tcttccacag ttgcagcttg
     7381 atgagatgtc aaacgtagct tcaactcttt ttcgtcagta actttccaag ttgtgtattt
     7441 taacgccatt taattaacct ccaacaccat ctgcaaattc caactctgac tgtaaagcaa
     7501 ttttaagggt gaaatcgata acggcattga caccgccacc gccaagtttt acggatactt
     7561 gaccttcaaa tgtgacctta gtaccgtctg gataagcttg ttcgaagtag agtttttcct
     7621 tgtcgtctgc tgccttacgc aatacacggt aaggagcagt tgcgcttgaa ttattataag
     7681 agaacttgta ctcaagttct cctacgtccc caataccaag ctcgtacttc ttcaccgtat
     7741 cttcaagagt agtattttct actttttcga gttcaatacc aaactctggc acttctttca
     7801 atccaacaag cttagtatag ctaccttttg ttttgctata agaaagcgta attccatttg
     7861 ctaacatata ttttaattct ccattctaaa ttgaaaaaca agctctgaat ctaagtcaac
     7921 gacaccttca aaacgcatga ccttatgtct caaatgagac gggtctggca cgtcttggca
     7981 gtcggttctt cgcaaaccta aagactcaaa aatctgattg attttaacag ctaactcact
     8041 agtgctggta tcatcaaaaa tatccacctt atagcggata gaggattttt gttccttgtc
     8101 gtcaaaccaa tcacctggct tgttttgttc ttctaaaaaa ataacgactg ggaaagtctc
     8161 ccaatcgcta ggataagtat cggtcacatt atctgcaacc ttttgcaatt ctttataaat
     8221 aacaggcttg atattgatca ttgtatttgt tctcttatct ttctacggac ataattcgaa
     8281 atattcttag acacacgctc ttgattgtct ctcaaagctg gataaagata aggctgagca
     8341 ggttgaccat acatcttgta aaactcccca atcttttgaa agtggtaaga tcctacattg
     8401 atttggtctt catgcacata ccaaggatga gacttgtaag tcacgctgac ctctggagag
     8461 ataccagaat ggctagcctg acccttaggt cccgttccaa actcaacgta aggagcgtat
     8521 ttaaggttgg tgtaaacttc gcctatagcc ctatctccgt ccatttttgc tctggttttg
     8581 atactagtta taagttctcc atcattcact ggtacgagtc ttcttgcatc agcttggaca
     8641 acttttatag cagcattgtg taccgcacgt aagacgatat cctcgccagt ttttttacta
     8701 gctaaccgtc tacatttagc tataagccta tctgccccta gtagccctga cacgctctaa
     8761 ctccaaaact tgatgatgtg tgtagacctt tttagaaata accctatgag tcacttccgt
     8821 ctgactatcg atacacacac catcttttac tttgatagta gctgacttgt tggcatttgc
     8881 gttcaaaata tcattgacac gctcgccata caattcagat tgtagcttgc tactagctgg
     8941 ccacaattca aggcggactg tctcagcttc cttggcatac ccttctttcg cgacaccttc
     9001 ctcagtgaca gtcttttcaa accgtcgcat tggatagggt ttcagtctac tctgcttcaa
     9061 aaacatgacc tgccaccctt gctagcctgt gcatgcgtat tcgctgtaga agacccgtag
     9121 ataggccgtt ttctccgtag actactgcta taccaccctc ggttctagaa tgctctcctt
     9181 ccgctcctga gcggttgtgg agctcgatag caacctcagg tatcaaaaga cttaaagcag
     9241 gtgtcaaaga tgtgcgatta gtctctgaca agataagatt tgtagccctc gtttggagca
     9301 acatgagaag ctgagtatct tcttcgcctg ttaatttctt cagcaactct atagacatat
     9361 caatcctctt ctaagaactc aggttcaggg aggattttct caagaacgtc tgagatagtg
     9421 acaccgttgc tggcaatatt gtcagccagc tcagcatagc gctcctcagt aatctcaagt
     9481 tcctctcctg ccagtcgttt cacatttgat tcccaatcat agaaatcttg tttgatttta
     9541 aatttcactt tttagttcct ccaaaacttc tacaatttcg gcttttgtca acttataagc
     9601 gcctgctaca cctgcttctt tcgctagatc cttcaactct tctagagttt tattctctaa
     9661 atcagaatac tggttaacct gctcttcttg aatataatga cgtcgtagca ataaactcat
     9721 atcgtcacct cttacgcgcc accgaatttt acaactcgtg tagggtcgta aaggtaaacg
     9781 ccgtagtgtt catcacctgt gattacggta gtcttcttca agatatcgcg gtctgtttcg
     9841 atagccacat cacgtttaag caagataaca aaagctccat atttgttagc gtcgtctgtc
     9901 tgagtttgac ttggtgacac tttaacaagg aagcctttac cttcatcaac ttttttagag
     9961 cgcacgattt gcacaccatc aacttcaccg aaggttccag aaacaaccat atttgctcca
    10021 agctctgaac ctttaatcca ttcttttgct actgcagttt ttagcttagt agcatcttta
    10081 gggttgatga tagcaacata ctgtgcatct tcttcgtcct caaagatatc tagagcttta
    10141 tcgattgcct caagagttgt tggagtttct gtaatatgtt gtgttgcagt tttagctacc
    10201 gctaccaaat cattatcgat cttgttggca atagctaaac caagctggta agtcgcttga
    10261 cctagtgggt caccaagacc tgacaaaaga gactcatcgg taatttcata acctttacct
    10321 gcttttttga tggtcatagt agtcttttta gtagtcaatt ggtctggcgt aattgcttgg
    10381 ccttcttcaa tttcagtagc gtctcctgca tactcccatg ctggcactgt taaagtgcta
    10441 cctggttgac cttcaagctt tgtctctaca tacgctagcg gtgtaaattt aatcaattta
    10501 ggtaatttag cggctaccat atccgccatt acttctggat taaccatagt ggctaattta
    10561 gtttgtcctg ctgtcatttt ctattatcct ttcaatttct tataaagctc tgggttcttt
    10621 tgatagagct catttcgact ctgataaccc atacgagcaa attcttcttt tgtgataccg
    10681 tcactatcga ctggcgcttg tttcattgga gctccgcctt ttagtttttc ttgaacgcct
    10741 ttttgcacgg cttgctccca tgatttctgc aatacagcga cagactgcga taccgtctct
    10801 gcgcttgtca aatcaactac atttactaac tcaacaggta agtcacgttc acttagcatt
    10861 gctttagctt ctgcggtcaa ttccttgcga gcaatagcct tctcacggtc agctagttct
    10921 tgctcacgct gatccaactg atatttttgt ttctcgtcag cgttcatctt ggcaagtttc
    10981 ttagcttcgt tttccttggc ttcttgctca gcttcccatt tagagcgttc ggcagatagc
    11041 atcttaccga tttcagcacg agtgaaagtt cgttcgtgct tttcttcctg cactgaatca
    11101 acatttcctt gagtgtcgac agtctcagtt gattcagtag atacagttgc attgatttct
    11161 tctgacataa ttgtcctcca gcgattacgt cgccactcga taatctcgtt ttacgtccgg
    11221 cgacggaaca gtacagcttt taatgtcatc ggtacagttt ggacaatata aaaaccgtac
    11281 ggggttccat acggttaggg cataagaaaa ccgcctcgat ttcgatgcgg tttatagcaa
    11341 tttatagcag tctattcctg caagtcaaga tgttggatca cctcctaatc tttaatggca
    11401 cgatttgaaa ctttggcgta aacatccaca taagtctctt tcttatctcc gttatgcgtg
    11461 atttctgcat aatttccaca aggttcgctt gatgtaattg cgttcgtact aacaagagct
    11521 ttccagtttt gcagggtctt gctaaaccaa actacaaagc agtcttctgc tttgatttca
    11581 cgacctgata agcgtgaaaa ttcttgcgat gccaattgtt ttgctttttc taacatttta
    11641 ttcctccgtt ttttcatatg tttctgcaaa aatatccagc ttgcatggat aatattctcc
    11701 ttgcacgcct ttgataatat aatccccttt tttagctacc atatcacctt caagtgtagc
    11761 aatccatata ttcccaagtg catcaaacca aatcttattt tctgcaaagt caattacctc
    11821 tttatggtta ttgccgttcc actgcacagc ctcgaccact actggttttt tacggtattt
    11881 cattcctcac tcctttctgg gtacgaaaaa agcacttaga tctctctagg tgcttaagta
    11941 gtgaattgca tttttatatt ttttaacacg ctcgtagtct gtattggtaa cagatttcaa
    12001 acgtgataaa tctgagttat gtttcaaatc tgcaagtttt acaactcttg ctaaattatt
    12061 tgattttact ttcccaagat attcttgata actttgacct tttttctttg tcaaaatttg
    12121 taccgctgta acaacttcat ttgacaagcc agacgcgaat aaatcggcag cagttatatc
    12181 gctatcctca atcacatcat gtaaaagagc gacagctttt tcttgttcag tgttgacttg
    12241 actggccaca tagagaggat gctgtatgta atcaacaccc gctttatcta cctgccctgc
    12301 atgtgctttt ttagcgatag ccaaggcaat atcaatcatg ccgctaccat cctgtcaata
    12361 taagtaaatg catcattttc tgaaatttct tcaaaatccg taaagtcatt aaaaaagatt
    12421 ttattaaacc aatccatgct attaacccac tttttttcaa tgtcaaaaac ttgcatgaca
    12481 ccatcaatca aacgaagtac ttgagcattg ttcgtcgttg tgcggtagta tttaatatct
    12541 ttcatatcac ttcaccctct ctatattttt aggaatctca agcccattgc ttaaatcaag
    12601 catttcctta aataatttca tgcgttctag atcagatgta ttcgtatcac gatacttctc
    12661 atagagttca tgtaatgaat cattttttaa gtcgaaactt tcctgagtat gatactgcat
    12721 ttcaaagttg ataccatctt tttcaacgac tgtattcaca cctttgtatg gtccatctac
    12781 tagccaagtg ttttttactt ttacaatttt ataaccttct gcgataagct tctgtttcat
    12841 cttcaaatac tcttttgcga aagtatcggg atcgaaaata gttgtatacc gcaaagcatc
    12901 gttaatttta cttgtagcct ttgataaact tatattttca gcttggctat ctgttgtaat
    12961 tttacgtgat aatgactcaa ctgttttctt cctaaattca agacctgcca atttgttttc
    13021 tcctgcgata cgttgcatat cacttgtaat ttttggctca actcctgaaa ttttggacaa
    13081 tagttgttcg ctataaaatt tcgccttggc ttctcttgta tcttgattat acaccttttc
    13141 cccgtctttc gcaacatact tgctatacca ctctttataa gtcatatcgg caggcacgta
    13201 ctcaacttta cctgtctctg gattccttgc tctgcgcttc aacttgctgt agtctgcgtc
    13261 ctcatcgtat ccgacagtag tagacctaca ccaagggtgc ataggcggac aattgacacc
    13321 agggacagcc ttatccctat catagacctg attgtcatgc tcctgacaaa tgcgtgatgt
    13381 acgcttgtct aagacggcca caaagatata cttttctatg tctgcttctt catagctgag
    13441 tagctccatc tggttatgaa aaaaggcgga ttctgttcga accaaacgcc ttgcatcgtt
    13501 ctgacctaca ttgaacctct cagcaattgc ttgtgcagtt tctcgtgtat ctctgcctgt
    13561 catgaggcta atgaggagtt catcttttat gctggaagta agttttcctg tattcttcca
    13621 gatgtctgtt gagtacgtac ttccatctcc tacccaacta aaagactgta gatgtttaat
    13681 ctcgttctca ggaagcccag aaaagccata tgctagtcct gtctgctgtt gtaggtcaaa
    13741 ggtagccttg tagtaactat ccttcatcag gtcgctataa aaggcatctg agcctgtctt
    13801 ctccgaatga tagatagatt cacgcatacg atctaaatcg tcgctcaaac gctctaggcg
    13861 cttcatgcgg aaagaataag ctgggctgtc taagtcagct agtaatcttt ggatgttcgg
    13921 gtcattcggt ctcgcttcaa gtactttacg aagttcattc agatttttct tgtctttcat
    13981 gttcttcaag acttgtctag cttctacctg actcagacca taatcacgtt ggaatttatc
    14041 gaaaatctta ttgatttcct tatccaagta agtcttagct tcctgataga ccttatcgaa
    14101 ctggtctgcc tgcttttcgg ccttgtccat ctgctggtaa atcagattgg ctttcctctt
    14161 cgcccaatac tcctgattct tcatcctcta cctcgtcttc gggtttcgtg ttgtctttgt
    14221 tgaacatcgg catgtcttcc atgttcttct ttttctcttc ttccaaggct tccagctcag
    14281 cgtcagggtc ttccacaaac ggcaagagag aaataagctg tctattggtc actttgcctt
    14341 ccaaattgtt cacaatctga gagatttcta ataagttctt aggcaaacca cgactgaatt
    14401 gtggaacgat tgaatgagac tctaaagcaa tctgcttcat gcctaagtaa tgagcaaaaa
    14461 tcgcaatacg ctgacgcaat cctcgcttat agttcgcttc cttggtctta gtaatcatct
    14521 caaggcccat cagcttaaat tccatggcta cgcctgatgt attccctgcg aaattctcat
    14581 cagtcaaatt aggcacatgg ctaaatgtgt agatgtcctc tttaagagct gtacgcaaga
    14641 tttcagtagc actttcgtcc agcgtattct tcaagaactc agcccttgca ctatcgcccg
    14701 gcaattccaa aagaccttct tcagaaagaa tcttcattgc taccttagcg tcttctggag
    14761 tgtctgctaa ctgcgtgcca tacaagacaa gtatagactc tacagcctgt tccttatcat
    14821 tgacacgatt ccccatcaag gaattataag cgtctatcaa gctaatttgt tgctcgtagt
    14881 cgccaatcgc aaagtgatta ttgcgatact cgataattgg gatttgacca aggttgtgag
    14941 gtgttgcctc ctcgctctga gttgttcctg aatctgtact tctcagcacc atgtgatagt
    15001 gcagattttc ggtaaagacc tcagcctggt gcttggtagt gtctttcgta tcgtctttta
    15061 cttcatagta atagaccgca aacaaaggct tccgctcaat actatcatcg tagaccatga
    15121 aagtattctc cggatcaata ctagttgaat ccaactcagt cataccctct ttagcataga
    15181 tgtactcgta agcacggccg tagatagcca tatttagtgc gttctgcgca tccacttggt
    15241 caatctcagc accatcaaag gctgtaagta gttcatcaat atcaccgtca gcagtattgt
    15301 tatacttgat aggattgccc ataaaatagc ccgtagccgt gtctgcgata tccttggcat
    15361 gattagctac cgtcttgtaa ttgggtgcgt tcacgttgcg tctcgtgtgt tttaagatag
    15421 catgctcgcc catgtagtag cttttaagct tcttcaaatg tgaactttca gtgttatgta
    15481 tcgttatcaa tttgtaaatc aggtctttct tcaaagaacc ctcatcatat ccatcccgtg
    15541 gataggttaa atattggtac atgtctttcc tctctataga ccataatcag aacgtctgcg
    15601 gacggttgct ttcccacctt cgatacattg aaggctgtaa cgcaaagcgt ccatcaagtg
    15661 gttgttttta tcctctggtt tattcaacca gttgccttct ttatctcgct ggtagcagta
    15721 actataaaat tcatccatga tgtttttaca atctggatgc acataaatag cgtatccttg
    15781 caatttggat acacctgcca taatactatc cttacctttc cgactctctt ttattctaga
    15841 tatgccatgt tctgacctga gctcttcaat cagtcgtaat tcagcgctat cagcaatgat
    15901 ttgtgagcga tgataacctt tgtcctttat catcttcgca acttctttgg ttatcaatcc
    15961 gactttatac gcctcatcaa agacataaat ctctttcgtc gtgtcattta tcaacgaaca
    16021 acacaaagca gttggatcat gagtaaaacc aaagtcaaga ccgatacata acttattagc
    16081 tgaatcttgt agtaattcat ctttattgaa atccttgaca gtcacgtttt cgtagattaa
    16141 accttcagca actccccatt cgccatcaca aacgattcta gcacgtctgg ggttcgtatg
    16201 atacaaatcc tcatagcgtt tgatatcgac ttcatcaagc cactcattgc atttgtaagt
    16261 ggttgtagta gcgaatgtgt cagcccgtct cgtctcttca tcgaagaata cacgcttgag
    16321 ccaatgcctc tcattccacg ggttaaatgt gactgtgatt tgtttaaaga aatcaggtac
    16381 atctaagcta ccacggatag actcaacaac cgtgctgaac ttgtcttcag tctcaatttg
    16441 atatgcttcc tcgaaccatg cccaacaaag actgccgaca tcgaccgtga tagatgtgat
    16501 tttgagttca tcatccaaac cacggaatag gattttttga ccagtcgctt ttatggttat
    16561 ttcaggcaaa gactcattga atttaaacaa atgagtcacc cccaacacat tacacgccca
    16621 tttaaaatcc gtataagttg attgcttatt tgtattcgaa tacctacgaa taacaagcaa
    16681 gttggcccag gaatatttca aaagacggac aacatagttt aaagcggttg tcttggactt
    16741 cttcgaacca cgggaccctt tgactacacg ataaagactt cttgagcgcc agaactgtcc
    16801 gtacccagct cctactgtct taggtaagtc aacgacaata tcgttttgct taatctggta
    16861 tgtctgactc atttgcaaac accaccgttc cagaaacatc cgcctctacc ttatccgtcc
    16921 aaagcctatg ccgtttacca agaagttcag ccgccttgat tctatcttta gctcctacat
    16981 ctatatccgt aatcgtctgc cctagttctc caatgcttat caaggtctgt tcttgcgtct
    17041 ctcctcgcat gactgaagtt agataaccta agacctcttg ctgatccgca atcttttcag
    17101 aatcaagctg tttcagtcgt tcatctatat agcttttaat cttaggattc tttagtaact
    17161 tatgtccttc aacgcctgcc actctatcac tagaaacacg ataacctgct ttcttatagg
    17221 cttccgtcgc attacctgag atgatgtact catctgcaaa tctcttttgt tttattctca
    17281 atccactcaa ttttccatca ccacctttca aacaatcaaa aaagccacac gatgtgcgac
    17341 cttcttgcaa ggcgactact accttgcgtg cgtattaaat tttgacttct tttttatttt
    17401 ttgtagtctt taaaacctct gagggaatca aaccctctag cttataactt atccggaata
    17461 taattagcta cgcaatcatg cgaggtccag tcgcttccgc aaccattttt aagttaatga
    17521 gtgataggag ttaatgagtg atatgtgaat ccccacccag aagatttaac tcattctggg
    17581 acacaaacac tcaaaggaga ggggaggact tgaacctcca aggccattac agccccctga
    17641 cattacaggt aaccatctac caattctgag acctctcttt tcaattcttg atactaccat
    17701 tctaacagat ttttagaacc gtgctgttcc aaaaagtccc ataagctcac tatgaggtta
    17761 gataacttct tccaaagcta agaccgcctc attttttaac ctgtagtagg ttgtacgact
    17821 catattcaaa tcataacaaa cgctatcagc ggtgcctttg ttgatgtaag tcattcttaa
    17881 tactgccctg tactttggat ttttaagcct attgatcatt ctacctaatt caagttttct
    17941 gttaatgacc tctttagtat cctgctctat agcctctttc atcactacca actgagtata
    18001 gacatcatca acttttctag tctgtccacc ttggacttta acctcggacc atttagggct
    18061 tgagagcaaa cctgcctcaa gctcgttaat ttcgtctata cggctttgga tgtccatgtc
    18121 cagatcctgc aactctttca agagctcttt agccttgttc actctctgtc tcctttgtga
    18181 tataatagtc tttgcgagaa ctattagctg aggcagagag tgtcttggct ttttttaatg
    18241 cttaaattcg ttgaccaggt cccggataaa gaacttccaa tcagattctc taaacgtcaa
    18301 gaaacgatct gtagtaaaat ttctaagtct tttatagaaa agcatcttta gttggattga
    18361 ctcaccaaca ctcagtaaaa taccggggaa gcgatgtact gaatgcactc tatttccgta
    18421 tccagaaata tctaaatgta ttaacgtttc tggatatatg cgccccatac tagcttcaac
    18481 tccgaactca accttaactt cttctacaat tggaacttcg tcaaaaattg gtcgtgcaga
    18541 aaatattggc gacggcgttt cttgtttttt tcttcttcct gaatatggat atttacaagg
    18601 tctcatttgc gtcctccaaa ctccttattt tcatagatgt tgccgatgat ttcattttct
    18661 tcaatttcag tccataaaca tactgcgtca ctgcccgtat caattaccca agaaccctca
    18721 agctgcttaa caatccctat aaattccttg tcatactcat agaaaccgcc aatttcgtca
    18781 gctctaccca aaaatctagt agttcgtaca atatcgcctt caaagatttc cttgccgttc
    18841 ttgtctttga gtcctgttga ttgcatgagt tcgatttcgt caaaatcata acaatagata
    18901 tctctatcgt ctggtaaacc attctcaaaa taaacttgtt gtgtcactat ttctttgttt
    18961 tcgtagtcaa tagcaagaat gtcatctgaa aaaaacatac gtttttctgt ttttatccac
    19021 gctctatatc ttggtgtcat gttaaatcct cctaagcatt aacaactgga aaatgaatat
    19081 caccaatcac taaagagcat acgctgtaat aatagccgtt atgctctgct tcacaattgg
    19141 caatagctac agggttctga ttatggaaga tagttacttt gtttttataa ccagttcccc
    19201 aatggtcggg gatttcttcc ggttctccaa tttcaatatt agtaatcaca gcgtcaagtg
    19261 atacatcttg gaactcccca cctgctgagg cacagcaatc actttcagac atttcaatag
    19321 tgacctttgt gccgtcttca agcagcaaaa agtccttatc ccatttcaca atacgcttaa
    19381 agagcaacaa ttctttaagc tcttccaacg acccgtacct tgcatttttc caatcaggct
    19441 cataatagtc tggtagttta atagtttctg tcatagtaac acctcatccc caactttcac
    19501 tttatcgtac acgtccttcg taaccacgaa caccccgtag tcacgaatgg taagcgtata
    19561 tagcttgcca tgccgtcctt tctcgacgac tttaccgaat atctcagcgc ctgcgttatc
    19621 agctttatag acgacaatcg ggcgcttttc ttctaacttt ttaatgtaga tactctgcca
    19681 gatattcaat ccagcagata gcagaatcca gatagctatg aatcgtttca atttatgacc
    19741 tcctccttca atttaagctc aatctctaag taaaagcttt gatcaggtat ctccagtatc
    19801 gctgtagtgg ttttaccatc agaaccaacg ataatttctc cgattgccaa aactaagtct
    19861 ccaattgtgc tatttagcgt aaggctcatc actccacctc ctcaatctca atccctggac
    19921 aatcaaacac ccagccgaag tttgcgtctt ctagttcttt ttgagtacct ttataattcc
    19981 tagctgttat atcttgacta aaataaagag tactccctga ttgcgatttg accaaaggct
    20041 gcctattttt taaagtcacc aaataccgct tctctttttc aacctcgtag ccgtcaagcc
    20101 aagcaagaca gaatttttcg atgttatttt cgtaaaacca ctcaggaact ttcttatcat
    20161 aatgatcttc aattactctc attgcaccgt aaacatgaaa attgtttttc tttttaaatt
    20221 ctatatattc cgccacacac tgcggaactt tgactggttt tggtttatct agttgttcca
    20281 agtcttgcag aaaaatttga cgagctagtt ctgctccttc agcattccat acaccctcaa
    20341 gttttttata tttctcaatc aattcctgct tattcatctt agtttcctcc ataaatcaaa
    20401 taaactgcaa taactacctg agccatgctt ggcgaatagc caatccaatc atcaaactcc
    20461 ttagattttg gcaaccaacc cttagtagct cccaaatcat agtctgtagg cttttcatca
    20521 gcaaagatgc attccatcgc tcccataaac gtcataccat cttctgccat ttcccaaaaa
    20581 tagtccgccc ggtctttcac cgcttgtggt aaatcttgct tgggaggttg cggcttcccg
    20641 tcttctaccg tccagttgta tacttcatta actttttgct ttaactcttc catcatcttc
    20701 caacttcttc actttccgtc ttaattctca ttatcttcct cctcaatttt aataacggcc
    20761 ctaccgtttg ggtgtcgtct ttggtgtgat gtgtaagtgt aatactttaa catcctttca
    20821 gtaattcctg tctcgctact gatctgcgct aatgttccaa gcgtaacaaa cacatcacct
    20881 tgatataatg cgtagtcagc catctgctcc tcatttcttc aaatactcag ggatttcatg
    20941 acctcaatct caacctctat ccgtggattt agactgtaga acttgcctac atcatgtagc
    21001 gctatctgac cgtcgtcctg gaagacgatc cctgacatgc tgtcatatag cgctttttcg
    21061 tagttatcta tgtcaggctt tttgcctact ggtataattt catccaggag ggcctgttgg
    21121 ttcttcttga ctttagaaat gtactgagga ggtttgatat aaaatctaag ccgtgccctc
    21181 aaagctccct caaggatagg ctgacccatg tactgattag caatgagcag ctggcaatga
    21241 ttgcgccatg ttttcatatc cttgtcttcg taagttgtgg taaaactccc acgtcttgca
    21301 aaccttggcc gtgattgagg tttaggctca atgttcaggg tcaatttcat tcaagagccc
    21361 ccttaaatcc tgccatctca aagagatttt ctctgttttc gtttacgaac tcaaagaatt
    21421 ttttaacctc ttgtagcgtc ttgatgttgc tcttgactcg tgttaatgag gtgaaaaata
    21481 catcattttt gggaattgcc ttaactttgc acttgtagac cggttcaaaa aggtcaccat
    21541 tgtcatctag tgtaggagcc gtgtctttgt tatcaaagct aatgctcata tcatagttta
    21601 gagtcgtaac gacctctatt ttttgtttat caatgatgat agcaatacgt tctgtcgcat
    21661 tgattttact tgccatgttc tttctcctgt taaaaaagtg tcgtttgcaa agggtacaca
    21721 tcttcaaacg gtactccaag tcttagacag tctcgtttga tgtccagtgt agagatgacg
    21781 tacttgacgc cgttgttttt cttgtcatag tgtggaaaag tgtacccgtc attttcaatt
    21841 ttggttttaa tgtccgtttt ggtttcaggt ttccaatcca cccaatccgt ccactccatc
    21901 tcatacctca tcaaataaac ttaattgagc gttgtggttt tttatccgct cctctgctat
    21961 accaaaataa tattcatcta tttcagttcc tataaaatgc ctatccgttt ctaacgctgc
    22021 taatgctgtc gtaccgcttc ccatgaaacc atcaaaaaca gtatccccaa catcagaatg
    22081 tttaacaata catcgtttta tcaattcaat aggtttttga ttttgatgga gtagtttatc
    22141 tgaacttact ctcttgaaat cccaaacatc tgtcagtcgc tcaccgttga atttcttccg
    22201 tcctttgttt gccaatataa tcatctcgta ctgttttcca aactgcgcct ctaaatcgcc
    22261 agcggtatga ttattttttc gccatataat aatatttttt actgaaaaat acttttcaat
    22321 ttctttttta aaaaaatcaa ccttgtcaaa tgaacagaat atataaatag ccgtatcatc
    22381 ttttaaaatt cgataacact cttttatata ctcttttatc aattcaggat tgttatcatt
    22441 tttgataaca tttgaaaatt tatgcttttc tttccgccaa tttgttttat aatttatcaa
    22501 ataaggcggg tctgttacga ttaaatcaat tttattatca ggaattgttt ccataaattc
    22561 caagacatct ttttttataa tcttatctat ttccactaac tgtccctcct aaaacggcaa
    22621 accgtcattt gggaggtcaa aggggttagg atcggtaaaa ggtgagctat tcccattttg
    22681 gaaactgttg ccttgtccgt gctgactgtt gcgactctct agcagagcta cactctcagc
    22741 gattacttca gtcacatatc gacgctgacc gtctttctcg taagacctaa cttgtaagcg
    22801 ccctgtgagg ccaataagtg agcccttgct gcaatactga gcaatgatgt cagctgtacc
    22861 tctccaagct tgaaaattga taaaatcagc ctcacgctct ccattttcgt ttttgaaatt
    22921 gcgattgact gcaagcgtgc cctgtaaact agatacatta ttaggcgttt ttcgtagatc
    22981 aggaggcgct acaagcctcc caaccagtgt gacgttattg atcatctgat ttgtccccct
    23041 ctagtgctac gctctcccaa gagataccct aaaaacatcc ataggatagc catcccaatc
    23101 tctttgataa aatcattcat tatttctctc ctttgcattc ataacataca ttttgaccta
    23161 catcttttcc cttgattatt gataagctac cacatttctc acagctgatt atgaaaccta
    23221 aaccatttga attaatactg cttatattgt tctctgaggg aactttgtaa ataatcaatg
    23281 cggatgtatg ccaatattca gcactgactc cactgtcagc gacagcagac acatttgatt
    23341 gaaatttgat gtcaatcaac ttaatgcctg gattttcggc aagccagcta tttatttggt
    23401 cgtcaatcgc ctcatgatgt ggataatcac atgaaaaaaa tacggtttta atcatattcc
    23461 cctcctggat tgtgccacca gatcatcagg tcttcctgat tatctctgat gtactgctca
    23521 aatatttcaa agtggacgat agcatgtttt aagcgttgca taccctctcc agattttgag
    23581 caaaagctga aaactttaaa gacaggctca atcatgtcaa taatttctac gacttggcca
    23641 ttgaggttcc agacgctatc ctctcccaca ttgaaatcaa ggataaactc atcccctagg
    23701 ttgtggataa cctgcaattt cttgccgtcc gagtagatgg ctacgctgtc agatattttt
    23761 ctgatgtcca tggttaccct ccccattgac tctggagaaa tatcccaaga tttttctatc
    23821 tccaatttcc ttttttcgcc atacagagtc gctaataggt cctctatttt tcctattaac
    23881 tcatcaggca ccccatattc agccaattct tctgaaattt tttcaatttc tgtcatactt
    23941 accacccaca ttgttcattt agctcagcct gagttaatgg atcgatacgt tgataaccgc
    24001 tgacttgata gttcttttta aaatcaaatc cgagtttact tagaccagcc ttgaaacagt
    24061 ctttttcggc tgtgtctaca aaatacacct ctaaagtcat tttttgggca tatcgtttta
    24121 ggtcattttc agcccctcta agagcgttag gctcattttg gaggatttgt ccaccgtcca
    24181 agattttgcc cgtttctggg tcaaaatttg gggtttccgt tgattttgga gcctgttctt
    24241 gctgtttggt ttgttgggct gctaaaagct cctgattaga tcgctctgct cgttctagat
    24301 cctgtctgag ttcttccttt tgcttttcaa actcataatc agttttgatt tgttcaaaga
    24361 cttcagcaag agtcaagtct ttcagctgtc taatgtaagg tgagtcagtc atgccgtact
    24421 cagcacataa ccctgaaata gctgacttgg ctttttcaaa ttcttgttgt ttctgaaatt
    24481 caaatgtgac catgtcatca agtgacttca tagtggcttt tttaagcgtc acgccatctg
    24541 ccataaaatc gctagctttg acatactcaa gggccttttc atcaaagaga cgaggatcca
    24601 gcatgtactc agctgatttg ttggctaggt agcctttgac tgtgtcaatt cggacagcct
    24661 tttgatgttc ttcaaactct ttgacatcac cagcaatttt ggtaatgatg tcttttagag
    24721 gctggatggc attcttgaca tacttgtcaa attcatcagc tggttcagat aagactttct
    24781 tattcctgat ccgttcatca gaaacctgct tgtctaattt tcgtagatcg gcaagtgtct
    24841 gcttgtcatc cttgatagtt gcagccgtaa ccgtgtaatt ttgatacttt gctacaacct
    24901 cattgatatt ctgctcaaat ttctcacggt caatgatttc aacctgtgct tgtgttactt
    24961 ttacctgtaa ttcttgcatg ttgtcctcct aatattcaag ttcaccgtct agcaactcgc
    25021 cctggattgg atcctcattt tgagtaggtt caggatctgc atgatttgcc tcttgctctt
    25081 tgttgaattg atcaatctga gccatcttgc gtgctacgac atcctcacgg ctctcttgag
    25141 gtgtgacgtc tttgattgcg tcaaatgtat ctccaccatc gatgtctgta tacatgttcc
    25201 caagttcgtc agggaaagcc tctctaagag tctgcactag agctgttttt ctgatcatag
    25261 tggctggcat tgatttccaa gtgctccgtg gattgccatt tctgtctgtt ttgacatact
    25321 cattaaagtc aactgtgacc ttatatctgt gtgaacggtc tttacgatag acaacggccc
    25381 aaccacctat tagaatgtcc tcaggtaatt taagagagcc ctcaatctct atcatttctc
    25441 catttctcat gacagtaatt ccagcctcaa aaccatcata tccctcacaa cgttcagcac
    25501 gtttcataaa tgcctctttt gagacaatca agctgaactc tgtgccgcta ttgttttgat
    25561 aggctacaat gtagacctcg ttagcaaatg ggttaagatt gcgaccttta cacaaggcta
    25621 gagcctgacc tacttgtttt tcagtcagta ggttttgtgg gtcaaaatac tttttgatgt
    25681 ctgccccagt caataaactt ggatcagtag tgatgtcacg ttttatctgt gttgctaatt
    25741 gattattagt catcttgttt tctcctatgt gttcatgtca attctgcgtc tagacttact
    25801 atttaagtca tctaattcat ttttgtaatc ttgaataagt tgcaaattcc ggtcaatgaa
    25861 gcgttctaca acttgaccta gaagttcttg tgttgtcacg cctctcaatt cagcaagaag
    25921 tctgatatat tctttttgtt tttcagagat ctctgctctt atgaacgact ttcctttatt
    25981 ggtcatctgc gtcatttttt tctcctttag gctagcaatc tcctacatag atccattgac
    26041 cagcgctgaa aatccaatca gctgggtcaa gttcttctcg ttcttcaggc ggttgcatta
    26101 tatctctgtc ataattaaac atgagcatac accttcccaa gctccagaac tcgtttcaca
    26161 tatctagcct tggatgttag cccaagatcc agcaattcgt ttttttcttc atgattggcc
    26221 aaaagccaga cacggttttc aatttcaatc tttgtcatct tcctgctcca cctcttcaat
    26281 tttcacttcg ctatttagac gtttcatggc ttcatctacc gacttgccgc ccaggacgtc
    26341 cttgagcatg tggcttacat cgtgcattgt ttgagccttc gccttgcttc tttcagtctc
    26401 tggcatcaat ccgacatctt gtagagccag aaaggctaaa ctgacatcgt gcatttcttt
    26461 ctgaagctgt ttgatttttt tgattgtttt tagtgcttta aacatattgt tctcctttta
    26521 ttctcctact ttccaaattc ggcaacgtga ctcaatttct ggtagttttt cattttgata
    26581 aacccaatcg ttaccatgaa cacctgatgc gatgtaagat atagatttta agtaatcaat
    26641 cgcttcttct tttgtttcaa aaactcttgc aatatagtct tggtgccccg tcggtaagaa
    26701 atcacgtcca atcaaactga aatcctcgtt tccagtttca gtattcttga cataaatcga
    26761 tataatgtac atctacactt ctccttgcag tctagccttg atatcaaagt tttctttgta
    26821 cttgtaggca gcaagctctt gtttcaaatc gtagttttct tgctcgaaag caaagcgacg
    26881 tttgcgctcc tcaaataggt cggtcatgag ttcgaccgct acctctcgcc agtcaaggtt
    26941 gactgcttta agaactactt caagtctgag ttttaacttg gtaagtaatt tcattaagct
    27001 acgccctcct cgttagattg cttgttcatg cctaaaataa tgtcatagta ggaatgacca
    27061 gcagggatga catagcctgt caaatcatca acttgagagc catctgccat gatgtttaca
    27121 attcttggtt tccattgctc tttttttctc ttcatgttat aattacctcg aatacttttg
    27181 ttgagcgcct gattgccgtc aggtgctttt tgttgtcttc tagactgtct tactttccat
    27241 cgccctgagt tctatctcat ggctgacttg tttcaatagc ttctcacacg ctattttagc
    27301 ttctctgtac gttgtagatt cgctgatgaa gtagtcagca agttctatga ttttatcttc
    27361 cattcaacct cctatatcag tcttgagact gatgtaattc cttcctaaat tgctataata
    27421 ctcttgacta ggacctctca ctgttttagt caaaatttca atagaaagga ggaaaattat
    27481 atgagtaaac ttagcagaaa accaaaccac catgttaaaa aacttacttg gtcagacctt
    27541 gataacattc tgatatctgc ttttacagag tcagcgactg atcatcctag tgcagtaatc
    27601 cgattatctg attatgagat gtctaaatcc gaaattatcg aagaagcaat tgcacaaggt
    27661 tacacagtta ttgacaattc tgatgacttc ttagagtttg aatagcgtgg gtcaaatatg
    27721 atatgttagt actatttgct tcaacatatc tttttaattt agcaatctgt tcatcggatt
    27781 gccttttctt tcttcctgaa tacggatatt ttcttggtct catttcctca cctcctttgt
    27841 atttattttt tctaccctct cttttattta tttagagaag taggactggt tgtcttttaa
    27901 tatttattgt tatttaatac ttgttgttag ttaatattta ttagtgccta aattttctga
    27961 tttgtaaaat acagatttgt aaaatacaga tttgtaaaaa tcggaaatgt aaattctaac
    28021 ctgtggataa cttagatata ctttcattca atctctgttt catgatttca aattgaaaat
    28081 cggatatttt tacatctgag aaaaatctga aaacacgaac tcctttacca cgtcccatgc
    28141 cttttttaac aattcgtagg tagccatttt tttctaatat tttgaagtag ctatcaactg
    28201 tgtctcgact aacacctttt cgtttagcta tttcatctgg atagacttgc caatttgggt
    28261 ggttagctag gacaaccatc ataataccaa cagctgtaaa gtccagtttt gggtcattga
    28321 taaaactatt actaacagca gtataatttt cagtcgcatt cttgaaagat aaattgacaa
    28381 tctaaatttt taaagtctgt catacgctct cctttctttt tttgtttaat ttgttaaaca
    28441 ttttctttaa aaaaaatctt tcacttgctt attaaaaact actgctaatt tttgaagtgt
    28501 tcgaattttc actgttgacg actgacctga ttcaattaag tgtattgttg ttcgagaaac
    28561 atttgacttc tctgcaagtt cctcttgaga cattttctct ttttcacgcc attttcttaa
    28621 acgttctcct tgcacgcact cacccccttg tgttaatagt tgccctgtcg tttctagata
    28681 gattgttcta atcattgaca ggcttggctt ttaattcaaa ttcaataata ctaagtcgat
    28741 cgattgcttc ttgcaattct tcggcttttt ttgatacttc tttacaggct tcctttagtt
    28801 cttcaatacc cgaaacttca acattaagcc gatatcctat tggtctcatt tctttctcct
    28861 ttttgatata atgtttaata aaaacgaggt ttgctatgtt aagtattgat acacaatttg
    28921 tagatacaat cagtaaaata ctatctgatt atgtttcaca ttctgaaatc acaaggatgg
    28981 gagaagtttt aggatatccc caaaacgacc agaactctgg actcaataaa caccatagag
    29041 ttcacaatat catgtctgat atactcaaca aaacacaaga taaatcaaat atcaaacttg
    29101 taattgagta tatctgcaat cctttgaggt acatcgatac ggtttcagat tttgaaaact
    29161 taagattaaa actaaatgtc gttctttccc taaaaggcct taccatatca gatgacggac
    29221 atgtagttat tactactgct tcacaaactt tagttgaggc aaagaaacga tttgaatcac
    29281 ttgaacatat gttgagaaca ttaaatgttc atccaaatgt tttaaaattc tgcacccagg
    29341 aactcttaca agaaaattat tttcatgctg tatttgaagc aagtaaagga atctttcacc
    29401 gcattcgttt gctaaccggt tcgtctctgg actcagcaag tctaatagac caatgtttta
    29461 aaaccaaaga acccatcatg attatcaacg gaaataaatt acaaactctc gacgaacaaa
    29521 gtgaatataa aggattgaag aatttacttc tgacaatcgc acatctttat cgtaattcca
    29581 aagcacataa acttaaatac tacaatccag atagcgttca tgatgcctta actgccttaa
    29641 ctcttatgtc cctcgctcac aatctccttg acaactgtac taatactagg agacttgatt
    29701 agtagattat aaaattctat agtcacctca gctaatctaa ttgtttcttc atctatggga
    29761 ctattgtagt cctcaaggtg atgaagcctt tcagtcaact gctctgataa gtattctgtt
    29821 tgcctaaaga tagatttatg aagatgagta attggtttta gcaacacgat ttcatcataa
    29881 gttaatattt tttcagtctc ctcttgagtt acttctgcta atttttccat accagaaata
    29941 tcaacattaa catgcggctg ttccattatc gttccccctc gtcttacttt ccagcgccct
    30001 gagttctatc tcatgactga cttgaagaaa tagcttctca cacgctattt tagcttctct
    30061 gtacgttgtg ttttcgctga tgaagtaatc agcaagttca atgattttat cttccaattc
    30121 taactacctt tcaaatgtgg tataatcaaa ataaaacgat tggagaaatc ttatgaataa
    30181 ttttagtttt atagaaacac ttgcgatcgc tgccatacct gcctttgttt ctggcatgtg
    30241 gtcttacatc gctgctaaag gaaacagcaa gcatgaaatt gataaaatta acattgcaca
    30301 ctcacaagag cttgaaaatg tcgaaaatca atttaaacaa gatatggaaa aaatgcaaaa
    30361 gcaacactca caagaacttt attcgctgca acaaactcac gaattaagat tacttgaact
    30421 tgaaaaagta tctcaactag acactcaaac cgaccaagga ttaaagataa atgacctaat
    30481 ttttaaagct atttcaggag aaatatctgc agatgtagca ataaaaaata taagcactct
    30541 tagtcatttc gcgaacaagc aacaaccttc cgatcttcaa aaacaattta tgaaaaaatt
    30601 atccaagaaa aatcacaaat gatacttttt cttgatacgc tctatctcag aatcaaacca
    30661 cattttgtgg tttttaattt tctttttcag atgatgctta ccagctaagt aagcaattaa
    30721 attcgtgata aaactagcaa tcattgatat gcctagtatc gtaagaaaag attcattcat
    30781 cgttctactc ctttctctct tttttcgctc tatgagcaac aacctgccaa ggattcgaac
    30841 cttggtgata ccaatcaggc tacattcatt ttatcaagca ttcctgcgaa cgctgcatcg
    30901 aaacgaatgt catcgatttc ctcttgagtg aaaccagaat cgagaaggta acgctcttgg
    30961 cgttcaatct catctgctaa ctctgtccat ccaaaagtga attgacggca gttagtactg
    31021 aatgtttcaa gttggctgta gaggaagttt tcctcgtaag tgccttggat taaagtttcc
    31081 ttagctactg ctttaaagat gttgattgct ttctcgttta atgtgttcat ggtgtttccc
    31141 tccggtttgt ttttgttatt tccttaagct tgattatagt ttaacacgtt aaacacaaaa
    31201 tgtcaagtgt gttaaacaaa aaaatttact ttttttattt aaagatgtat aatggattaa
    31261 acaatatata gaaaggggtt ttaaatgaag ttaggagaat tgctaaaatc atatagaaca
    31321 gagcataaat tatcaatgga tgctttttgt gaattatctg atttaacaaa aggatatatt
    31381 tctatgcttg aaaaaaatga acatccgaaa tcgaaaaagc ccattgtccc atcttatgac
    31441 acaatagaaa aaattgctaa aggaatgcaa atttctacag aagatttaat tgatatgctt
    31501 gatgatgatc aagaaattca aatcaacgct actccagctc ttctttcaaa atccccaatc
    31561 caaaccatct acgacgaact agaaccacct agacagggca aagtcctgaa ttatgcaaag
    31621 aggcaactgg acgagcagga aaacgaagaa gaaacgaaga taaacgaagt atcggagaac
    31681 atcatcagac tggacgacta cagacagact acttaccgac gtgttactgg ggttgtctct
    31741 gctggtagtg gttcgataca ggacgacgat ttagatatgg aggtttcgtt ctatgaggat
    31801 gaaatcccag acgactacga cgctatcgct tatgtcgtcg gcaactctat ggagccaaag
    31861 ataaagaatg gtgactatct ttttataaag aatacccaac aggttgatta taacactatc
    31921 ggcatcttcc aagtagacgg cgctaactat gttaagaaac tgcgtcaggg atatctggaa
    31981 agcttgaatc cagattatga ggatatacac ctagacgaaa gcaacgacat ccgaactatt
    32041 ggggaagttg tcagtgtgta tagggagaaa taacatgagt aacgaaagta gaccgatgga
    32101 agtgattaaa cacaacctag attgcaaatg ccacagacga agagagtgga ttagagtaaa
    32161 tgataaatgg catgctatcg agttttcggt agacgatcca aacgaacctc ctatgacaga
    32221 ggaagagaaa gccaacgtgg ccttaattct tcaacaacac ttatcgaaag aataaaacca
    32281 actgtttcca aaatggaaat aattgcaaac aaaaaaagcc ccacgctctc aaaactttgg
    32341 cgagtctgag cgtgaggcat gtgacaggaa aagattttca tggagataac ctctcatgat
    32401 gtcttttctt gtacccattt tatcattttt taggaaattt tgaaagaggt actactatga
    32461 taacaacaaa taaagtagct atatatgtca gggtatcgac gacaaaccag gttgaggagg
    32521 gatactctat agatgagcaa aaagacaagc tctctagcta ctgcgacatt aaagactgga
    32581 atgtatacaa agtatatact gatggaggtt tctcaggatc caacactgac agaccagcgc
    32641 tagaaagtct tatcaaagac gctaaaaaaa gaaaatttga cacagttcta gtctataagc
    32701 tggaccgtct tagccgtagt caaaaagaca cgcttcactt gattgaggat gtattcatca
    32761 agaatgggat tgaatttctg agcttgcagg agaactttga cacctctact ccttttggta
    32821 aggctatgat tggactcttg agcgtctttg ctcagctaga aagggagcaa atcaaggaac
    32881 gcatgcaact tggcaagcta ggacgtgcca aatctggtaa atccatgatg tgggctaaga
    32941 catcctatgg ttacgattac cacaaagaga caggcacagt gaccatcaat ccagctcagg
    33001 ctctgaccat taagtttatc tttgagagtt acctgagagg gagatctatt actaagttga
    33061 gagatgatct aaatgagaaa tacccaaagc atgtgccttg gagctatcgg gcggtcagaa
    33121 ccatactcga taaccctgtc tattgtggtt tcaatcagta taagggagaa atttatccag
    33181 gtaatcatga gccgattatt tcaaaagagg aatacgataa gactcaatct gagctaaaaa
    33241 taagacaaag aacagcagca gagaatgtca atcctagacc attccaagct aagtacattt
    33301 tatccggtat cgcccaatgt ggatattgtg gcgctccttt aaaaattatg ttaggcgtaa
    33361 agaggaaaga tgggagcagg ttaaaaaaat atgaatgcca tcaaaggcac ccacgaacgc
    33421 tgagaggcgt tactacctac aacgacaata aaaagtgtga ctcaggattt tactacaaag
    33481 acaagctaga ggcctctgtg ctaaaagaaa taagcaagct acaagatgac gctgattacc
    33541 tggacaaaat attttcagga gacaatgctg agaccataga ccgtgagagc tataagaaac
    33601 aaatagagga gctatcaaag aaactgagca gacttaacga tctatacata gatgaccgca
    33661 ttacccttga agaattacag agcaagtcag ccgaatttat aagcatgagg gctactcttg
    33721 agactgaact ggaaaacgat ccagcactca ggaagaacaa aagaaaggct gatatgagga
    33781 aactgctaaa cgctgagaaa gtcttttcaa tggactacga aagtcaaaag gtgcttgtta
    33841 gaaggcttat aaacaaggtt aaggtgacag ctgaggacat tgttatcaat tggaaaatat
    33901 aaataatttt agtaacctac atttccacca atgtaaaagc tttaacctta gcttttttca
    33961 agaatgtcat catttttttc attttaaaaa tttacctcca tattttgata catgggcatg
    34021 agcattgccg cataaagtaa aacgataatc agtgccacaa agataaaaac cagtggctgc
    34081 accaaattca tggtgcggtt gactcgggta aaaaaggctt cccaagtttt ttcagcatag
    34141 atttccaact cactacccag cttggactta acttccccat actctatgat aagactcaat
    34201 tccttcctaa agaaaggata ggttcctatc gtctgagaaa attcacggcc atttttcagg
    34261 gtttgagcca gatcttgacc gatttcttta aagagctggg aaccttgttc ctgcatcatt
    34321 tgaaaaatct gcgtcagctc cattccctgt gaaatcatat tcccccattc acgtgcataa
    34381 taggctgtca agtaggtctg cacaaagatt ccaataaagg gaaggcgtgc taagatagaa
    34441 aagacactca tcttagaact tcttttataa aaagttagtg ctaaaagggc aagcacggaa
    34501 acaagcccta ccatgcctag aaaaatttgt ggcagattac cgataattcg ggtggcaata
    34561 ttgctactat ccagttgtgg gagcaggtaa ttccgtagcc ccagcataat taagagaaga
    34621 aaacccagca aaatcaaagg ataggtcgct acttcaatta actttttctt gaccttggcc
    34681 agattgtcta gatattcttc tatctttccc aaactcaggt gaagattgcc atgaacttca
    34741 gctagagata actgagtgac aatggtactt gaacatccca aactttccat catttctgag
    34801 aatgatttcc cctgagacaa gcctgtacgc atctgggtca cacactgctt gtccaacaaa
    34861 gcactcctat ctaaaaagga gatggtctcc accagatgaa aaccgctaga aaagagattg
    34921 ttaaataggg tgatgatatt tttttgctta gctgtagcta attttttccg tctcagcctg
    34981 aagacttgtg atatgtccat ctttaagaag ctggtcaatt tgctcattcc acttggctgc
    35041 ttggtgttct tgataatctc tgcttgcaaa gtcaacgatt cctcctcccc cgattaatct
    35101 ctggtagcag actccttgca gaacaactgc caattcttct tcactcacac ccaactccag
    35161 cagacgctca taaacacctc ggatactttt ggcgtgaatg gttgaaaaga ctgtcgcacc
    35221 tgtcaaacta gctctgacca ctgcacgcgc cgtctcgctg tcacgaattt ctccgataat
    35281 caagagatct ggtcgatgac gcaaggaaag tttgattaga ttttcatagg ttaggccgat
    35341 tgcttcgttc aactgcaact gaagcatgtc gtcctgcttg atttcgacag gatcttcgat
    35401 ggacataact tgctgtcctt taaagagtga cttggacaat tcatgcatca aggtcgtctt
    35461 accactccca accggaccag caaaaagata gagtccccgt tgcctgtact gcttgcctaa
    35521 ttcttcaata tcctgaaacc aaaaatgcaa gtcctgctcc tcatcgtgca acaaacggat
    35581 aaccaaactc tcatgccccc gataatcgcc tacagtagat aaacgtagag acgctatctt
    35641 ctggtcatag gcataatcac aggaacccag ttgactacgt cttttttctc ccacattcat
    35701 acccgccaca aacttaaagt gactgataac ggctgcaaac ttttcaaaat catagctacc
    35761 aattttacag cgctcgtctc ctaccctcat atgaagctca taggcatcta acttagggac
    35821 aaaataaatg tcttgcgccc cttttttccg agccgaacga atgatttctt gtgcaatttc
    35881 ttgaaccata cttacctcct cacctatact attcgcaaag cgttagaaaa ataaaaaaag
    35941 caactccaaa aaagttactt ttctctattt taatttcata cggcaagaac gattcctttc
    36001 ctgactggtt tgtttttcat agcctgagcg tagtttttcg tctgggataa cctgctcatc
    36061 ctgcaaaaga gccaaagaat ggtattgttg taaatactca tcacattcat cacaccagcg
    36121 ttggtcttcc gggtcccaaa aaatggtcat caagtcgctt ctacttttat aaagagggga
    36181 ttcttgttcc aatctaaacc agcaagtttt gtagtatttg agagcatcat aatactggtc
    36241 aaattttcta cttgctacaa tatcttcttc ccaaccttct aagaaccacc acggttcaaa
    36301 atctccgtac atttctataa cacgatacat attcattcat tcctttgtac cgtatattat
    36361 aaccaattcc gccagacaag gaaagaaata tgctcatttc ttaatttttt gataaaaaat
    36421 ccagccatct gatggatgac tgaatttcta tttacttatg tacaaaaata tttttactac
    36481 ttaggcttct gagatatcgt tttcgataat gaccttaatg gcatggtggt ctgctgcctt
    36541 actgaagact tcgtaggctt tttcaatttc actgagtttg aaatagtgag ttaccaattt
    36601 ttctggttca atcttatgac tttcaagtgc tttcaacaat tgtggagtcg tatttgtaga
    36661 taccaaacca gttgttacat tgatgttgcg aatccaaagt ttatctaaat cgaattcaac
    36721 tggtttacca tgcacaccac agttggcaac cgttccgtct acaccgataa tcttttgaca
    36781 gaaatcaaat gttgcaggaa taccaacagc ttcgatagcg acatccacac cacgaccatc
    36841 tgtcaaatca taaatttctt taatggcttt ttcagggtct gaagaattaa ccttatgagt
    36901 cgcaccgaat gatagggcag tttccaagcg gttatcgtct aggtctacca taatcaattt
    36961 agctggtgaa tagaattggg ctgttaaaag agcagccaat ccaactggac ctgaaccaat
    37021 aatggctacg ctgcaaccag gttctacttt cccttttaag acaccaattt catatccagt
    37081 aggcagaatg tctgacagca taaccaaagc ttcatctgac aagtcttctg gagtatggta
    37141 aagagtatta tctgcatgag ggacacgtag atattcagcc tgcataccat caatcaagtg
    37201 accgaaaatc cagccccctt cgtcttcaca gtgggcataa attccttttt tacagtagta
    37261 gcatttacca caggcacaga cgcaagaaat caagaccttg tcgccttttt tgaagttgga
    37321 aactccttcc ccaacttctt caacaatccc aatcccttcg tggccaagaa tggtaccact
    37381 ttggcaagta ggaacatccc ctttgataat atggaggtct gttccacaaa tagtggtttt
    37441 tacaatacgc acaatagcgt ctgttggctt gcgaataact ggtttgtcta catcaacaaa
    37501 agaagcaagt cctggtttaa cataagtata ggctttcata aagcactcct ccgttttttt
    37561 atttgtaata tttatagttc aattgtaagc ctttttcttt gtttcttcaa gctttttttg
    37621 tgattttttt aactttttat ttaccagtaa agtaccctgt ataaaaaagc agaagctagt
    37681 ctctaacttc tgccttctct cttatgcttg ataacgtttt acgccatcta gataggttgc
    37741 taccaattcc aaatctttat ctaatacgat gaagtcagcg tcgtagcctt cacggatttg
    37801 gccacagacg tcatcgatgt gaacggattt agctgggttg aagctggcca tcatgactgc
    37861 ttcatgcgga ttcgcaattc cccattcgac cacattcttc aaaccatctt tgagtttgag
    37921 gatagaacct gccaagttac ctgtcgattt gaggcgtgca gttccatttg caacgacaac
    37981 tgggaattct cccaacatat aatctccgtc ttccaatccc ccagctgtca tacagtctgt
    38041 gataagagcg atgttttctg ttcctttttg tttgataaga atttcgcaag cctttggatc
    38101 tacgtggtga ccatcacaga tcaactctgc ataggtatgt ggcaattggt acatggctcc
    38161 aaccataccc aattcacggt gagtcaaccc acgcattcca ttgtaggcat gcacccaaac
    38221 actcgctcca gcatcgactg cttttttggc ttcatcaaaa gtcgcgtttg aatgtccaag
    38281 agcaaccgtc acaccttcgc ccgtaactgt acgaacaaag tcttccaccc catcacgttc
    38341 tggtgcaagg gcaattttat taagcaagcc atttgccgct ttttgccaag aatgaaactc
    38401 ctcaacaccc gggtctctca tataagttgg attttgtgcc cccttaaaag tttctgtgaa
    38461 atatggacct tcataataaa tcccacgaat cttagcacct gttgcttctt tataatggtt
    38521 tccaagattt tcagtgactg caagcaattg ctcataagtg gctgttaaag ttgtgggtaa
    38581 gaaactggta acaccggtac taagaagtcc ttcactcata gtatgcaatg taccttcaat
    38641 gttgttgtcc atcacatcta cacctgcata tccatgaata tgagtatcca caagacctgg
    38701 ggcaatgcta taacctgtat agtcaatcac ctcagcccct tcaggaatct gctctacatg
    38761 tttcccaaac ttgccgtcca caagttccaa gtaaccacct cgacgaactc cgtgtgggta
    38821 gaaaaactga tccgctttaa tatagttagg cataatgtta acctccttaa aagattgatt
    38881 ctacaattta ttatgtcaat tacattataa acctttcttt tttatttgta aatggtatag
    38941 acctattttt tggagatatt ttaaaagagc tggatttctc caactcttct cttttttaat
    39001 acaagttatt ttgatttaac cggcttgtct tgagctgtct gcaaagctgt ggcaatcgta
    39061 tctgcataca attttgctcc tgcttcgata gtgctactct cactcccgaa atgaacctgg
    39121 tctgttccag cccaaatttc tggatgctct ttcgcaactt gattccaatc tgctatcgta
    39181 atgtaaggtg tcttctctgc caattctctc atataggcag cagccttctc aacgatggca
    39241 taggtctctt ttgtcttatc tccctcataa ggagtcacca aaatcatatg gtgtccctta
    39301 ggaagatttt tcacgatact gtcccagtca tccttgtaat tctcaggatt atttacccca
    39361 gtcgcaatga ccaccgtctt aggtaaaaat ttattctggc tattatttag catgatttca
    39421 tttgcggtct tggttgttac gctgacctgc gcgttaatct gtgctccagg aagagctgtc
    39481 tgtagtgctg tatttgccct taaagccact gagtcaccaa ttaacatagt gccatcagca
    39541 attcccaaac tgtttgcatc tgcccgttct gccatcacct tggtctggcc aatatttgtt
    39601 gcagcttgct tcaagccatt gacagtcaag tctgtctcaa acgctcccac ttgtggtgcc
    39661 aacaaggtca ccgtgcagac aatgatggtc aagattcctg tacctgctac aagaattgcg
    39721 tgaatataag gcaggggacg aagagtttgt aaaataggtg tgttcttgcc tgcaatccaa
    39781 ggttccaata catagaatga cagactggca aagccataag aacaaatcag agtcagtaat
    39841 acagcaagaa gatttgatgt caactgtgag aaaatgatat agaaaggcca atggaaaaga
    39901 taaaccgcat agctagtatc cgctaaaaag ctgataatct tcggctcctg tatatgatgt
    39961 gtcttttcat gtaagacacg cgccgccaga atcatggcaa gagctgcaag actggcaagc
    40021 aagaagccga taagataggc aaaaagataa gtgaatttga caaagaaagt caaaagaact
    40081 aggaaaccaa agcctcctcc aaaaactacc aaagtctttc gtaaatccca gattttatcc
    40141 aactgcttga cgagggaagt cgtctgacga acacctacaa tagttgctaa catacttccc
    40201 aaaaagaatg gatagacatg agttaaactg gagaaataaa cagaggaata agaggtcact
    40261 agaaaactac caataaacat ggagaagaaa ctgatcaaga aggcagcagc agataagaga
    40321 aagaccatcc ccttcaactg accatttgat ttagcttgtt tggataagaa ccaaactgcc
    40381 aatccccaaa gaatatagta gtgaacctca actgccaaac tccaattatg aacaaacaaa
    40441 tgaggaatga actgagattc ataactccca cctgttagga gttcatagaa gttggtcata
    40501 aagcctaaga cgcccgcaat ctggccacca attccagcaa catagtcttg gcgaaccaag
    40561 aaagtaaaag gcatggtcac caagaccatc aaaaccacag gtggcacaat ccgataaaag
    40621 cgtcttctaa aaaatcctat caaatcaatc tcattgttct tagaaaattc ttcaatgagt
    40681 agagctgtaa tcaggaaacc tgaaaatgtg aaaaagacat ctaccccgaa aaatcctcca
    40741 ggaaagatcg tctgaaagaa gtgatacaag agtaccaaaa gtaaacctat aatcctaatc
    40801 aaggaaaacc atttaatgcg catacgagtt tattcttcct ttcattgtac actttattct
    40861 atcaaaaaaa gaagaaaaat cctaagagaa aacttaggat ttttttatat caattccatt
    40921 ttagaaatta cgtcctgact tattatagcc atatttttcc acaaaatact cacggaattc
    40981 caagagattg tcatccatga tggcttgtcg cacttgcttc atcaggttaa gcaagaagta
    41041 aagattgtgg tagctagtca agcggatacc aaaggtttca tcagccttga gcaggtgacg
    41101 aaggtaagcg cgtgtatagt tattacatgt gtagcagtca cactcaggat ccagtggcgt
    41161 aaagtcctca gcaaactggg catttttcac aaccaaacgt ccttgactgg tcatacaagt
    41221 cccgttacga gcaattcgag tcggtaagac acagtcaaac atatccaccc cacgaatgac
    41281 cccatcgatc aagctatctg gcgctcccac acccatcaga taacgaggtt tattttcagg
    41341 tagcagttga gttgtaaagt ccaagaccgc attcatctct tcatgggttt ctcccactgc
    41401 caaaccaccg atagagtagc ctgagaaatc catgctgaca agatcatgag ctgattggcg
    41461 gcgaaggtct tcaaatcctg caccttgcac aattccaaac aaaccttggt catgtggacg
    41521 acggtgagcc ttcaaaccac gctcagccca acggctggta cgctcgatcg atttcttaac
    41581 gtagtcataa ggttgataaa actgaggaca ttcatcaaag gacatcatga tgtctgaacc
    41641 cagattattc tgaatagaga tggctttttc tggggatagg aacatcttag aaccatttag
    41701 atgattttta aaggttactc cttcttctgt gatattacgg ctatctgcta aagaataaac
    41761 ctgaaaacca ccactatctg tcaagatagg ctggtcccaa ttcatgaact tgtggagacc
    41821 accagcgcgt gcaatgagtt catctccagg gcgaagccag agatgatagg tgtttgatag
    41881 gataattccc gaacccatct ccttcaattc ttcaggtgac tgagttttga cagtggcttg
    41941 tgtcccaact ggcataaaca taggtgtcgg aaaggtaccg tggggagtga tgatttctcc
    42001 cagacgagct cctgtgtgtt tttctttctt aatcaaacga tatttgattg gtgaatctga
    42061 cattttttac ctccgaagct gggaaagaca gtcccagttc atacagtgtg cccaaggaca
    42121 cactgtatga ttttatcaaa aaaagtagga actgtcacga actatgtata agaactatgg
    42181 tataataaga gaatgaacta tccaaaaatt gatttaaaaa caattcgtca ggagtcaaaa
    42241 cattttcagg ctgacacccc ccgtctcttt ctcctctata tccttcccag tatgctggtc
    42301 atcttatctg gttttcttaa tcccttatct cggatccacg ggactgtttt agaacagcca
    42361 tttttcagca tacttggcca aatacttcaa acctacctct ttcctctact agtctccttt
    42421 atcgggacta ttcttttgac cagttccgtc tatgcaacac tgacactcat gaaagattct
    42481 aaaacggagc catctgtaaa aaacagccta gctctctttg atgaggagcg cttttctcaa
    42541 acctttttga ccctacttct caaacgtttc tatctctttt tatggagtat ccctaattta
    42601 cttgggattt acctcctctt ttatagtagc tttttagcca aaaaattcgt caccctacat
    42661 cctgagtttc caaatctaga tctctcatca gttgaaaccg agcgtttcct catggtcttt
    42721 ggactctact ttcttgcaag tctcatcttg atcattgtag gaaatatcct ctatattcca
    42781 cagtattatg cctattcaca ggtggaattt cttctctgtt atagtctaga cctaggacag
    42841 gtcccaccaa gacgaatcct aaaaactagc cgttccttta tgaagggcta caagttccag
    42901 cactttgtcc tagatttaca gcttcttcct tggtacttcc tcaattggat tacctttggc
    42961 attgctagtt tttcactcct accttatatc caatgcacta aaattatgtt ttaccgagca
    43021 gtattggccc gaaaacgtcc aaaagcttga aggtttttcc cctcaagctt ttttctatca
    43081 atgagtttct ccgcctacca acttgagttc ctgatctcca tgttctatta tagcgccgat
    43141 tgctgcttct atccctcgcc gaatatccac taaactcata gctggagtag tcggtctgtt
    43201 caccacctgt tccatcatat aaggaatatg cataaaacct gccttaacat atggagattt
    43261 cttttctacc aaatagagag cctgatacat caaatggctg cagacaaaag tccctgccgt
    43321 attggaaaca gaggccggta agccctcttt ttttatagct tgaaccatcg ctttaatcgg
    43381 caaactacta aagtaggccg aagcaccatc tgggcgaatg ggacggtcaa tcggttgatt
    43441 atcttcgtta tcagaaatgc atgcatcgtc ttgattaatg gtcactcgtt caggtgtcaa
    43501 actagttctt ccaccagctt gcccaataca aaggacaaag tcaggttgat aacgattcat
    43561 ctcttcttcc aatacttgag cagatttgtg aaaaactgtc ggcacctcta gccaacggac
    43621 ctcagcacca tggatttcag ctggtaaacc tttaatggcc tccaaagctg gattgccctt
    43681 ttcccctcca aagggctcaa aacccgtcac taatactttc atttctaact cctcaaaacc
    43741 aattcaatga gacttttttc ttaaaaacaa gtataacata tttcccttat tctggattaa
    43801 aaaggattag cgagattaga atcgaccttc aagtgtattt aaagagtaaa atactaatca
    43861 aggccaaaat agctgatcca ccttgtttca aaataatttt tttatccgct gttaaagagc
    43921 cgtaagtcgc agcaccaatc acaaataaga caaaaatagt cacaatttct aaattctgtg
    43981 agaaataaat gacatacaag agaaagactc ctagcagagc cttataaatt ccttgatttt
    44041 tgaacaatga acttactgac ggatgagcca attcttcctt ttccatatta aatacacgac
    44101 tagtcgcatc tgattgcgtg gcaatacttt ccaaataaaa aatgtaaaaa tgctccaaag
    44161 caacgatcgt tgttaaaata attgacatct atttctcctt aaatttctat attttcaata
    44221 cccaaaacca atttatttaa taaacggctg agttctgttc tctcaaactc tgttaagata
    44281 ctttctatct cttccttaac cctgatatgt tgcagagggg gattcaccac taactgctcc
    44341 ttggcatact tcgcagcctc taccaacact tcccgctgat tttcaggatt acgatgacgc
    44401 tccaccaaac cttccgtttc caaaattttg aaatgccgtg tcaaagcagc ctgatcaatt
    44461 ttcaaacgct cctgaaccgc catttggtta caaggagaat gctccagcaa aaacagtaaa
    44521 atctgatacc gtgtcaaact aatcccaagc cttttttcaa acaattgcgt actcgcttgc
    44581 tcagacaagc ggagttgata cagtaaatct tgaatctcta gcatttcttc ccctctcttt
    44641 ttattgattt atcaatattt gactaattaa ttgtgatata aagtaaaata gattgcaaga
    44701 atgttgctta actcattaca agatgataac tttccatttt ccattcaaat tgatactcaa
    44761 tgaaaatcaa aaagcaaact aggaagctag tcgcaggttg ctcaaagcac agctttgaag
    44821 tttcagataa aactgacgtg gtttgaagag attttcgaag agtatgacaa agaaaaaacg
    44881 aacgagaatc gctttccatc acgaaagcta gatctcattc gtcaaaatga tgttatcaag
    44941 tcgggtttat ccattcattg aaacgtgaac gtgatcatag tgattttctg tcacactacc
    45001 acggtctggc attgggttcc aagtgttagc tggcccatat ttgctatcga atggagcata
    45061 gaaacgttgt ttccagatga tgtaactaat gccacggctg gccatatttt gaatagcata
    45121 ttccgcaatc ttatccccta attctgaacg ttctggtacc ataaagtcga tagccaaacc
    45181 ttttccgtga tctccactgt ctcctggacg ataaccacta aaggatgtaa tgccaaacaa
    45241 gttagcaatt tcttctttaa aggcagctgt ttgtggttga agacctgcat tttcagattt
    45301 tgctactgca agtccagcat aatcgggcgc agctggagcc tcgtaagttg ttgaaattat
    45361 tttcgtctct tctggttgat aagtagaaac tgctgctgta gctccatttg atgatgctac
    45421 ttcttttgct tctgaacttg ttgtagttac ttccactgct gatgcagctt gagggcttgc
    45481 ttgtgtttcc tgcttctcag ctggagtcgt ttcctgagga gttgcttctt caactgggcg
    45541 agttgtttcg gtcgtttgct cctctgggac agaagtgccc gtagatggtg ccacttcttc
    45601 agaagcaatc actgtctttg taacttctgc aattggttga gaaaggtctg caacctgaac
    45661 agtttgatca tcaacggtca cttgattagt ggtcaaatct gctgtcgcag ttgtcacttc
    45721 ttcactagag tctgcttgag gtgtttggat ttcaacttct gttacttctt ctgcttcatt
    45781 gacagtcgtt gtcaaaacag tttctgggaa aatcaagtcc atattagtga ttttgttcag
    45841 attcgcaagc actgttacat ctacacccaa ggcttctgca atggtgctca aagtatcacc
    45901 atactgtacg gtataacttg ttttgttgtc cgttttagtc aaatcgtttt ggatttgctc
    45961 aacactacgt gcagtccaaa gaacttcttc tgcttgagtt gccaatactg gggcaaatga
    46021 caaggctact gttgacgcta ataacattct tttcttcatt ctttcaaatt cctttcaaat
    46081 gagtacctgt ctatcataac acaaaaaatg tagaaaaaaa gccctctcgg gcctatttaa
    46141 cagaactgtc tattccttgt aacaaactag gttacttgaa atagtttgtt aggtctctgt
    46201 cacaaaactg acacaatctt tttatcactt agtctcaaaa atacgggaaa tatctgctaa
    46261 cgcttgaatc ctgtgattcc cttcataagt agactctaaa aagttgatgc tttgaatccc
    46321 actattctgg gcaaattcca catccagagt ccgatcccct atataataag tattatcaga
    46381 attcaactga tacttatcta gcagataggt agccgcttct ggacttggct tccgcacaaa
    46441 gccactctga ctggttaaaa tctctgtaaa ataggattcc acccccaagt ccttgagaat
    46501 ggtaaaagcg ttgttcccct tatgagtata tataaactgc tgaattcctg attcgtctgc
    46561 ccaagctagc acctcacgcg cacctggcat caaaactacc tgagcattct tctcagccag
    46621 actctgggca cgcacctgat ttagcacctc aacatccaga tttctatctt ctgccacccg
    46681 cacaagcaaa tcttgcaccg aatacttgaa gataaactct ctcaccttct ccttatcata
    46741 aggaatagaa aactgagcaa aagtctcctc aatccctgat aaaatcgctt cgtaagagtc
    46801 caataaagtc ccgtctaaat cccaaataaa agctgttttt tgcatttcct atcctttcaa
    46861 gctcatatag cgctggtaac gatagcgtag aaataaccag cgaaaaccat tatccaagag
    46921 agaccctgcc caaataccag gcaagcccca accaagcaca atccccatca gatatcctgt
    46981 cccaatgcgg atacaccaca ttcctatact tgtcgcataa aaagggaggc gtgcatttcc
    47041 taatccctgc cagactgccg tatagatgac tgttcctgtc gtcatagggg tcccaagtag
    47101 tgaaaacagt gtcactagaa cactagcctc caccgctaga gaatcagtcg tatagagatg
    47161 agttaatggt acacccaaga catatatact aaaggacagg ggcaacatga ggaacagaga
    47221 aagccaaaag gtttgtttac tcaaactagc aactcttttc caatcatcct ctccaactgc
    47281 tcgggccaac agcatgaccg ttgccgtagc gacgccaaag gcaggcatat agttaaactg
    47341 ggtcaagact tctccgattg cattcccagc aactgcctcc gtcccaaaag aaacgaccaa
    47401 ggcaatgatc actacatctc cagccctcat cataagtcgc tctccagctg ctggtaaagc
    47461 caaggtcaac agttccttat ctaaaccaaa agttggcttc ccataaggca gttttaattg
    47521 tgaccacaaa atcacaagac caaccaaacg agacacaatt gtcccccaag caacaccagc
    47581 tatccccata tccagaacaa aaatagctag acttgaaaaa agaatattca aggcattgga
    47641 taaaaaacta acatagagag gcagacgtgg attatgcgtt gcacgaatca aggctcctag
    47701 actagtcatt aaacctaaga gaacaatcga tccgcctacc aaagatagat acagtccacc
    47761 actctcagct acatccctct ccgtccccaa aagtcctatc atctctttcc cagcgaagat
    47821 ggacaaaaat cctaaaagga aacttaatag taaggtaatc ttcaacgcct cagtcacatg
    47881 ataggccaac ttcgactggt ctttctgccc tatgcttttt gaaataacac tggaaatagc
    47941 agctcccaga gcgatgaaaa tcgcctgata aatggtgata atattaccag ctactgaaac
    48001 ccctgaaata gctatcaatc ctaaatgagc aaccaaataa ctgtccacca ttcccattag
    48061 catctgcaaa aagttttcac ccatagctgg caatgcaata ttaagaatgt ctttattttt
    48121 cttaaacaat ctctcctcct gatgaaaaga aactcagttg gtttcccaac cgagtttgct
    48181 ccctctgtct taaagtccta aataagcctc aaccgctgct tgcatgtcag cagctgccac
    48241 tgttgtcttg tgacgaattg gagctatttc aagcccatca actgctggtg gcactgcaac
    48301 gcctgagatt tcatgtaatt gagccaaggc ttcaaagtct gttaaacctg cttttccagt
    48361 tacagcttct actgcaacta ctgggaactt gtatggacta gctgttgaag caatcactgt
    48421 cttagttaca tctccagtgg ccgattggta ttttttataa actgctgaag caacagctgt
    48481 atgagggtcc tcgatataag aatctaactc acaaacacgc ttgatctctg ctgccgtttc
    48541 ttcctcagtc gcatattcag ctgcaaagag gtccaaaatc tctgcatcaa agtctgtcaa
    48601 cttatattgt ccttgcgtgt tcaaggcatt cataagttca gttgtctttt cagcattatt
    48661 tcccaaaaga tggaaaatca agcgctccaa gtttgaagat accaagatat ccatagatgg
    48721 gctggttgtt accttaaact cacgtttttt gtcatagaca cgtgttttaa agaagtctgt
    48781 caaaacattg ttgtcatttg aagcacagat taatttacca actggcaaac caatttgttt
    48841 ggcataaaag gcagccaaga tatttccaaa gtttcctgtt ggtactgtga agttaacctt
    48901 ttcaccagct acaatttcac cagtcttaac caattgagcg taagcataaa cataataaac
    48961 aatttgtggc accagacgac caatgttcat agagttagct gatgaaaatt gcaacttgtt
    49021 ggtagtcaat ttttcacgaa gagccacgtc gttaaacatg tgcttcacat ttgtttgcgc
    49081 atcgtcaaag ttaccatcaa tagcaataac atgagtattg tcgccagtct gagtggtcat
    49141 ttgtaactct tgaatcttgc tgacaccatc ctttggataa aagacgataa tctcagtacc
    49201 aggcacattc gcaaaccccg ccatagcagc tttccccgtg tcaccagatg tcgctgtcaa
    49261 gataacaatc ttgttctcca aaccatgttt cttagcagca gtcgtcataa agtatggcaa
    49321 aatagacaag gccatatcct taaaggcaat cgttgaacca tggaaaagtt ccaaattgta
    49381 ttgcccatct aatttcacta atggtgcaat agctggagta tcaaatttgc tatcgtaggc
    49441 attgttgata cagtagtcca actcctcaac tgtaaagtca tctaaaaatg ctgacaaaac
    49501 tagcttagca acttcctggt aagaagcatc tttcaatttg tcaaagttca aatctacctt
    49561 tggataagta tccggtgtaa acaaaccgcc gtccgtcgcc aaaccttgca aaattgcttg
    49621 gctggcagtt actgtattgt tggcatcacg cgttgattga taaactaatg tcattactct
    49681 ctatccttta tctatagtct cgtccattat atcatgattt ttcagaaaat tctcccttta
    49741 taagagaaat tataggctca attcttctct caaaggctgt aaataggtca tttcttcctt
    49801 atttctcacc actagtccct cctcagctag taggagtaag tctttggaaa attcaataat
    49861 cgtagtttct tcctcatctg taagattttt cttagaaaat tgtctcctta aagacttgta
    49921 atcataacca aatactttaa agaaaggtgc tgtttctaag taagcttcta acttgtctaa
    49981 attaaccaat aatcccaagt gaaaagctgc agaagcaaaa gtcctatcaa gtggctgtgt
    50041 acacacacta cgaaactcaa ctgttcctcg agtcgttaaa tcttggtact ggtaactacg
    50101 atgagtttca aaatccttct cttgggggta aataataacc tcatccccat tcagagcaaa
    50161 tgcttggatt tcggacgtag ccaaatagtc cccagcctga ataggataaa aataataggt
    50221 ctgcccatca cgttccgcag taaaaatcgc agaatgattt agatagtcaa aaaaatcagc
    50281 ttcatcatta aggagtctag cattgacccc aacattctct ggatagatac catgcataga
    50341 ttcttcccag aaaatatccc ttgaaatttt cgtatcccaa tccgcacccg aaaattcaga
    50401 gtttgcaaat aaataagcct tagccgcttc aatttgagta aaagcattaa tcacccgtaa
    50461 gtagttggtt tttgaaatat ccagctgaac ctggctccca cagataaaag taccatattc
    50521 agggaaatga tgtaaatctg atttaataat atttctactc aaattcaaat aatccatcaa
    50581 catctgatag cgtggataag ccactggaca attctcattt ttatcccagt tgggatggat
    50641 accacagcca acaatagcat gatttgattc agctaacttt ctctgaatta cattcatata
    50701 attattgaaa cgattttcga cctcttgaat cgtttcagcc ttaccaaatg caaactcaat
    50761 cgttgtatag gaaacttcaa ataaaatagc atcctgactt atcggatcta ctaactggat
    50821 cagattgcca aaatcatcta cctttgctac ggtgagatcc aaagtagaaa ctaaataatg
    50881 aaatagatcc ttcataactt caacatctgt agcatcccct tctaaacttg caacaggata
    50941 ctccaactca attccgacaa acaattcagg attttcttta atatttttca aataccgatg
    51001 ttttaataaa tcgatagaac aagacatact cccctacact ttcttaatct aaataaaatt
    51061 tgaataacta taattatacc aaaaataata taaaaaagga acgaagaata acaacgttcc
    51121 taacactata ctataccggc ggccggggtc gaaccggcac gtccttgcgg acactggatt
    51181 ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tagtaactgg ggtagctgga
    51241 ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaat
    51301 aatataaaat aggcgagtga tggggatcga acccacgcat gccagagcca caatctggtg
    51361 tgttaaccac ttcaccacac ccgccataat tctattaaca cgggcagtag gaattgaacc
    51421 cacactgaag gttttggaga ccttagttct acctttaaac tatgcccgta aaatggaagg
    51481 ggagggattc gaacccccga acccgaagga gcggatttac agtccgccgc gtttagcctc
    51541 ttcgctaccc ttccaaaata tataaatggc gcgagacgga atcgaaccgc cgacacatgg
    51601 agcttcaatc cattgctcta ccaactgagc taccgagcct tattgcggga gcaggatttg
    51661 aacctacgac cttcgggtta tgagcccgac gagctaccga gctgctccat cccgcgttaa
    51721 tataaaagga ggatgtggga ttcgaaccca cgcacgcttt tacacgcctg acggttttca
    51781 agaccgttcc cttcagccgg acttgggtaa tcctccaaaa tagtttatac gggaacaagc
    51841 ccgtgtcctc ttagtgaaca gataatattc caatccccta gtaatggact aaacactatg
    51901 ggcacgagtg gactcgaacc accgacctca cgcttatcag gcgtgcgctc taaccacctg
    51961 agctacgcgc ccaagtcaaa aacttggtac aaagaacaaa gttcaaagcg ggtgacgaga
    52021 atcgaactcg cgacaacagc ttggaaggct gtagttttac cactaaacta cacccgctaa
    52081 a
//
LOCUS       DAVKLW010000007        51887 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKLW010000007 DAVKLW010000000
VERSION     DAVKLW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.007"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..412
                     /locus_tag="VW324_000408"
     CDS             <1..412
                     /locus_tag="VW324_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9726092.1"
                     /translation="LFDQVATLLEEREIVTPTYREALITREKSFPTGLDMEFLGKDLP
                     NVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNIL
                     AQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            450..755
                     /locus_tag="VW324_000409"
     CDS             450..755
                     /locus_tag="VW324_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9726093.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            803..2278
                     /locus_tag="VW324_000410"
     CDS             803..2278
                     /locus_tag="VW324_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9726094.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2583..9284
                     /locus_tag="VW324_000411"
     CDS             2583..9284
                     /locus_tag="VW324_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9726095.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9532..10388)
                     /locus_tag="VW324_000412"
                     /pseudo
     CDS             complement(9532..10388)
                     /locus_tag="VW324_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10599..10958)
                     /locus_tag="VW324_000413"
     CDS             complement(10599..10958)
                     /locus_tag="VW324_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9726096.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            11050..12006
                     /locus_tag="VW324_000414"
                     /pseudo
     CDS             11050..12006
                     /locus_tag="VW324_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11990..12547
                     /locus_tag="VW324_000415"
     CDS             11990..12547
                     /locus_tag="VW324_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9726097.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12540..12794
                     /locus_tag="VW324_000416"
     CDS             12540..12794
                     /locus_tag="VW324_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726098.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12806..14860
                     /locus_tag="VW324_000417"
     CDS             12806..14860
                     /locus_tag="VW324_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9726099.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15039..15899
                     /locus_tag="VW324_000418"
     CDS             15039..15899
                     /locus_tag="VW324_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9726100.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            16110..16820
                     /gene="ccdA2"
                     /locus_tag="VW324_000419"
     CDS             16110..16820
                     /gene="ccdA2"
                     /locus_tag="VW324_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9726101.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16840..17406
                     /locus_tag="VW324_000420"
     CDS             16840..17406
                     /locus_tag="VW324_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9726102.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17417..18529
                     /gene="msrB"
                     /locus_tag="VW324_000421"
     CDS             17417..18529
                     /gene="msrB"
                     /locus_tag="VW324_000421"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9726103.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEIMLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18797..19534
                     /locus_tag="VW324_000422"
     CDS             18797..19534
                     /locus_tag="VW324_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9726104.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19531..21222
                     /locus_tag="VW324_000423"
     CDS             19531..21222
                     /locus_tag="VW324_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9726105.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21338..21901
                     /locus_tag="VW324_000424"
     CDS             21338..21901
                     /locus_tag="VW324_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9726106.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            21923..27592
                     /locus_tag="VW324_000425"
     CDS             21923..27592
                     /locus_tag="VW324_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU9726107.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            27653..29374
                     /gene="pabB"
                     /locus_tag="VW324_000426"
     CDS             27653..29374
                     /gene="pabB"
                     /locus_tag="VW324_000426"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9726108.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            29494..31020
                     /locus_tag="VW324_000427"
     CDS             29494..31020
                     /locus_tag="VW324_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9726109.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            31107..32026
                     /locus_tag="VW324_000428"
                     /pseudo
     CDS             31107..32026
                     /locus_tag="VW324_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            32161..33120
                     /locus_tag="VW324_000429"
     CDS             32161..33120
                     /locus_tag="VW324_000429"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9726110.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFELAKEEDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            33217..34056
                     /locus_tag="VW324_000430"
     CDS             33217..34056
                     /locus_tag="VW324_000430"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9726111.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(34104..34274)
                     /locus_tag="VW324_000431"
     CDS             complement(34104..34274)
                     /locus_tag="VW324_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9726112.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            34395..35279
                     /gene="miaA"
                     /locus_tag="VW324_000432"
     CDS             34395..35279
                     /gene="miaA"
                     /locus_tag="VW324_000432"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9726113.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            35272..36510
                     /gene="hflX"
                     /locus_tag="VW324_000433"
     CDS             35272..36510
                     /gene="hflX"
                     /locus_tag="VW324_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9726114.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            36503..37126
                     /locus_tag="VW324_000434"
     CDS             36503..37126
                     /locus_tag="VW324_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9726115.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            37141..38070
                     /locus_tag="VW324_000435"
     CDS             37141..38070
                     /locus_tag="VW324_000435"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9726116.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            38091..38846
                     /locus_tag="VW324_000436"
     CDS             38091..38846
                     /locus_tag="VW324_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9726117.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(38900..39808)
                     /locus_tag="VW324_000437"
     CDS             complement(38900..39808)
                     /locus_tag="VW324_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9726118.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            39872..40141
                     /locus_tag="VW324_000438"
     CDS             39872..40141
                     /locus_tag="VW324_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9726119.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            40141..40521
                     /locus_tag="VW324_000439"
     CDS             40141..40521
                     /locus_tag="VW324_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9726120.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            40610..40837
                     /locus_tag="VW324_000440"
                     /pseudo
     CDS             40610..40837
                     /locus_tag="VW324_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40877..41602)
                     /locus_tag="VW324_000441"
     CDS             complement(40877..41602)
                     /locus_tag="VW324_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9726121.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            41764..43605
                     /gene="typA"
                     /locus_tag="VW324_000442"
     CDS             41764..43605
                     /gene="typA"
                     /locus_tag="VW324_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9726122.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            43625..43879
                     /locus_tag="VW324_000443"
     CDS             43625..43879
                     /locus_tag="VW324_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9726123.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            44243..44542
                     /locus_tag="VW324_000444"
                     /pseudo
     CDS             44243..44542
                     /locus_tag="VW324_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            44613..46634
                     /locus_tag="VW324_000445"
     CDS             44613..46634
                     /locus_tag="VW324_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9726124.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASFFILRSRDWVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            46636..47277
                     /locus_tag="VW324_000446"
     CDS             46636..47277
                     /locus_tag="VW324_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726125.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLDLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWGQVDQVIRVTDLSHR"
     gene            47381..48733
                     /locus_tag="VW324_000447"
     CDS             47381..48733
                     /locus_tag="VW324_000447"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9726126.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            48735..49793
                     /locus_tag="VW324_000448"
     CDS             48735..49793
                     /locus_tag="VW324_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9726127.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQALFSKGG
                     FVSVPPVIAACVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            49803..51023
                     /gene="divIB"
                     /locus_tag="VW324_000449"
     CDS             49803..51023
                     /gene="divIB"
                     /locus_tag="VW324_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9726128.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYISVLFNDSEQIKAFT
                     SELAQINPELKVAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
BASE COUNT        16077 a         9401 c        11418 g        14991 t
ORIGIN      
        1 tctctttgat caggttgcaa ctttattgga agaacgagaa attgtaactc caacttatcg
       61 tgaagccttg atcacgcgtg aaaagtcatt tccaactggt ttagatatgg aatttctagg
      121 aaaggacttg ccaaatgtag cgattcctca cacagatatt gttcataatc tagctgagaa
      181 agtggtggtt gttcgattag aaaaaccagt aacttttcac aatatgatag ctcctgataa
      241 ggaagtagaa gtatctctac tcttctttat cattaacaat tcaagttcaa gtcaaacgaa
      301 cattctggct cagttgatgg actttttcac aggaaatggt catcttgaag acctatcaaa
      361 aatttctgaa ccagaaaaac tttatgctta cattgctgaa gcaaccgctt aatcttgtct
      421 attaaaaaat aaaatcggag gaaatccaaa tgattaaaat tcttgctgcc tgcggtgcag
      481 gtgttaactc aagtcaccaa attaaaagtg ctctagaaga agaactttca aaccgtggtt
      541 atgatgttca ctgtgatgca gtcatggtga aagatgtaaa cgaagacctt atgaaaggtt
      601 acgatatctt tacaccaatc gctgcaacag accttggttt tgaaccaggt atcccagtta
      661 tcgaagctgg gccaatctta ttccgtatcc cagcaatgag cgctccagta tttgacaata
      721 ttgaagcagc tattaaagaa catggattaa gctaattcta atttgttaac attcatataa
      781 cataaaaaag ggaggaactg ttatggatgc aatctttgac ctaatcggaa aggttttcaa
      841 tcccatctta gaaatgggtg gacctgtcat catgttgatc attttgacag tattggcttt
      901 actttttgga gtgaaattct ccaaagcgct tgaaggtggt atcaaacttg ccatcgctct
      961 tacaggtatc ggtgctatca tcggtatgct aaatggtgct ttctcagcat cacttgcaaa
     1021 attcgttgaa aacactggta tccaattgag tattacagac gttggttggg caccactcgc
     1081 aacaatcact tggggttctg cttggacact atacttcttg ctcatcatgt tgattgtcaa
     1141 cgtagtgatg cttgctatga agaaaaccga tacacttgat gtcgatatct ttgatatctg
     1201 gcacttgtct atcacaggtc tcttgattaa atggtatgct gataacaatg gtgtgagtca
     1261 aggggtttca ctctttattg ctacagcagc tatcgtcctt gtcggtgtgt tgaaaattat
     1321 caactctgac ttgatgaaac ctacatttga tgaccttctt aacgccccaa gttcatcacc
     1381 aatgacatca actcacatga actacatgat gaacccagtt atcatggttt tggataagat
     1441 ttttgaaaaa ttcttcccag gccttgataa atatgacttt gatgctgcta aattgaacaa
     1501 gaaaatcggt ttctggggat ctaaattctt catcggtttc atccttggta tcgttatcgg
     1561 tattatggga actccacatc caattgcagg tgttgcagat gcagataaat ggcgtcttgt
     1621 tatcaaagga tggttgtctc ttggtttgac tgccggtgta tctttggaac tcttctcact
     1681 tatcggttca tggttcatcg cagccgtaga accactatca caaggtatta caaacgttgc
     1741 tactaaacgt cttcgaggac gtaaattcaa tatcggtctt gactggccat tcatcgctgg
     1801 tcgtgctgaa atctgggctt gtgccaacgt acttgcacca atcatgttga ttgaagcagt
     1861 gcttctttca aaagttggaa atggtatctt gccacttgca ggtatcatcg ctatgggtgt
     1921 tactccagct ctcttggttg taactcgtgg taaattgctc cgtatgatta tcttcggaac
     1981 actcttgttg ccactcttcc ttctttcagg tacacttatt gcaccatttg caacagaact
     2041 tgctaaaggt gtaggtgcct tcccagaagg tgtgagccaa actcaattga ttactcactc
     2101 tactcttgaa ggaccaatcg aaaaacttct tggttggaca attggtaaca ctacaactgg
     2161 tgatatcaaa gcaatccttg gtgcagtagt cttccttgta ttctatatcg gtatctttgc
     2221 ttggtacaga aaacaaatga tcaaacgtaa cgaagagtac gcagcaaaag caaaataatg
     2281 cgctcctata aaatataaac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag
     2341 gttgctaaaa acacagtttt gaggttgtag ataagactga cgaagtcagc tcaaaacatg
     2401 gttttgaggt tgtagatgaa actgacgaag tcagtaatca tatctacggc aaggcgacgc
     2461 tgacgaagtt tgaattggat tttcgcagag tgtaaattgt tgaaactagg ttgtcatacc
     2521 atgtatacca cttgggcttt ggtagtagct aactgcacta aatataatat aaggagggtt
     2581 ttatggggaa aggccattgg aatcggaaaa gagtttatag cattcgtaag tttgctgtgg
     2641 gagcttgctc agtaatgatt gggacttgtg cagttctatt aggaggaaat atagctggag
     2701 aatctgtagt ttatgcggat gaaacactta ttactcatac tgctgagaaa cctaaagagg
     2761 aaaaaatgat agtagaagaa aaggctgata aagctttgga aactaaaaat gtagttgaaa
     2821 ggacagaaca aagtgaacct agttcaactg aggctattgc atctgagaag aaagaagatg
     2881 aagccgtaac tccaaaagag gaaaaagtgt ctgctaaacc ggaagaaaaa gctccaagga
     2941 tagaatcaca agcttcaagt caagaaaaac cgctcaagga agatgctaaa gctgtaacaa
     3001 atgaagaaat gaatcaaatg attgaagaca ggaaagtgga ttttaatcaa aattggcact
     3061 ttaaactcaa tgcaaattct aaggaagcca ttaaacctga tgcagacgta tctacgtgga
     3121 aaaaattaga tttaccgtat gactggagta tctttaacaa tttcgatcat gaatctcctg
     3181 cacaaaatga aggtggacaa ctcaacggtg gggaagcttg gtatcgcaag actttcaaac
     3241 tagatgaaaa agacctcaag aaaaatgttc gccttacttt tgatggcgtc tacatggatt
     3301 ctcaagttta tgtcaatggt cagttagtgg ggcattatcc aaatggttat aaccagttct
     3361 catatgatat caccaaatac cttcacaaag atggtcgtga gaatgtgatt gctgtccatg
     3421 cagtcaacaa acagccaagt agccgttggt attcaggaag tggtatctat cgtgatgtga
     3481 ctttacaagt gacagataag gtgcatgttg agaaaaatgg gacaactatt ttaacaccaa
     3541 aacttgaaga acaacaacat ggcaaggttg aaactcatgt gaccagcaaa atcgtcaata
     3601 cggacgacaa agaccatgaa cttgtagccg aatatcaaat cgttgaacga ggtggtcatg
     3661 ctgtaacagg cttagttcgt acagcgagtc gtaccttaaa agcacatgaa tcaacaagcc
     3721 tagatgcgat tttagaagtt gaaagaccaa aactctggac cgttttaaat gacaaacctg
     3781 ccttgtacga attgattacg cgtgtttacc gtgacggtca attggttgat gctaagaagg
     3841 atttgtttgg ttaccgttac tatcactgga ctccaaatga aggtttctct ttgaatggtg
     3901 aacgtattaa attccatgga gtatccttgc accacgacca tggggcgctt ggagcagaag
     3961 aaaactataa agcagaatat cgccgtctca aacaaatgaa ggagatggga gttaactcta
     4021 tccgtacaac ccacaaccct gctagtgagc aaaccttgca aatcgcagca gaactaggtt
     4081 tactcgttca ggaagaggcc tttgatacgt ggtatggtgg caagaaacct tatgactatg
     4141 gacgtttctt tgaaaaagat gccactcacc cagaagctcg aaaaggtgaa aaatggtctg
     4201 attttgacct acgtaccatg gtcgaaagag gcaaaaacaa ccctgctatc ttcatgtggt
     4261 caattggtaa tgaaataggt gaagctaatg gtgatgccca ctctttagca actgttaaac
     4321 gtttggtcaa ggttatcaag gatgttgata agactcgcta tgttaccatg ggagcagata
     4381 aattccgttt cggcaatggt agcggagggc atgagaaaat tgctgatgaa ctcgatgctg
     4441 ttggatttaa ctattctgaa gataattaca aagcccttag agctaagcat ccaaaatggt
     4501 tgatttacgg ttcagaaaca tcatcagcaa cccgtacacg aggaagttac tatcgccctg
     4561 aacgtgaatt gaaacatagc aatggacctg agcgtaatta tgaacagtca gattatggaa
     4621 atgatcgtgt gggttggggg aaaacagcaa ccgcttcatg gacttttgac cgtgacaacg
     4681 ctggctatgc tggacagttt atctggacag gtacggacta tattggtgaa cctacaccat
     4741 ggcacaacca aaatcaaact cctgttaaga gctcttactt tggtatcgta gatacagccg
     4801 gcattccaaa acatgacttc tatctctacc aaagccaatg ggtttctgtt aagaagaaac
     4861 cgatggtaca ccttcttcct cactggaact gggaaaacaa agaattagca tccaaagtag
     4921 ctgactcaga aggtaagatt ccagttcgtg cttattcgaa tgcttctagt gtagaattgt
     4981 tcttgaatgg aaaatctctt ggtcttaaga ctttcaataa aaaacaaacc agcgatgggc
     5041 ggacttacca agaaggtgca aatgctaatg aactttatct tgaatggaaa gttgcctatc
     5101 aaccaggtac cttggaagca attgctcgtg atgaatctgg caaggaaatt gctcgagata
     5161 agattacgac tgctggtaag ccagcggcag ttcgtcttat taaggaagac catgcgattg
     5221 cagcagatgg aaaagacttg acttacatct actatgaaat tgttgacagc caggggaatg
     5281 tggttccaac tgctaataat ctggttcgct tccaattgca tggccaaggt caactggtcg
     5341 gtgtagataa cggagaacaa gccagccgtg aacgctataa ggcgcaagca gatggttctt
     5401 ggattcgtaa agcatttaat ggtaaaggtg ttgccattgt caaatcaact gaacaagcag
     5461 ggaaattcac ccttactgct cactctgatc tcttgaaatc tagtcaagtc actgtcttta
     5521 ctggtaagaa agaaggacaa gaaaagactg ttttggggac agaggtgcca aaagtacaga
     5581 ccattattgg agaggcacct gaaatgccta ccactgttcc gtttctatac agtgatggta
     5641 gtcgtgcaga acgtcctgta acctggtctt cagtagatgt gagcaagcct ggtattgtaa
     5701 cggtgaaagg tatggctgac ggacgagaag tagaagctcg tgtagaagtg attgctctta
     5761 aatcagagct accagttgtg aaacgtattg ctccaaatac tgacttgaat tctgtagaca
     5821 aatctgtttc ctatgttttg actgatggaa gtgtacaaga gtacgaagta gatagctggg
     5881 agattacgga agtagataaa gccaaacttt cagtagctgg atcacgtatt caaatgactg
     5941 gtcagttagc tggagaaact attcatgcaa cccttgtggt agaagaagga aatgctgcag
     6001 cacctgtagt gccaactgtt actgttggag gtgaagcagt aacaggtctt actagtcgac
     6061 aaccaatgca atatcgtact ctatcttatg gtgcccaatt gccagaagtc acagcaagtg
     6121 ctgaaaatgc tgatgtgaca gttcttcaag caagcgcagc aaacggcatg cgtgcaagca
     6181 tatttgttca gcctaaagat ggtggccctc ttcaaaccta tgcaattcaa ttcctagaag
     6241 aagcaccaaa aattgctcac ttgagcttgc aagtggaaaa agctgacagt ctcaaagaag
     6301 accaaactgt caaattgtcg gttcgagctc actatcaaga tggaacgcaa gctgtattac
     6361 cagctgataa agtaaccttc tctacaagtg gtgaagggga agtcgcaatt cgtaaaggaa
     6421 tgcttgagtt gcataagcca ggagcagtca ctctcaaagc ggaatatgag ggagctaaag
     6481 gccaagttga actcactatc caagccaata ctgagaagaa gattgcgcaa tctatccgtc
     6541 ctgtaaatgt agtcacagat ttgcatcaaa aacctactct tccaacaaca gtaacggttg
     6601 agtatgacaa aggtttccct aaagctcaca aagtcacttg gcaagctatt ccgaaagaaa
     6661 aactagactc ctatcaaaca tttgaagtac taggtaaagt tgaaggaatt gaccttgaag
     6721 cgcgtgcaaa agtctctgta gaaggtatcg tttcagttga agaagtcagt gtgacaactc
     6781 caatcgcaga agcaccacaa ttaccagaaa gtgttcggac atatgattca aatggtcacg
     6841 tttcatcagc taaggttgca tgggatgcga ttcgtccaga gcaatacgct aaggaaggtg
     6901 tctttacagt taatggtcgc ttagaaggta ctcaattaac aactaaactt catgttcgcg
     6961 tatctgctca aactgagcaa ggtgcaaaca tttctgacca atggaccggt tcagaattgc
     7021 cacttgcctt tgcttcagac tcaaatccaa gcgacccagt ttcaaatgtt aatgacaagc
     7081 tcatttccta caataaccaa ccagccaatc gttggacaaa ctggaatcgt actaatccag
     7141 aagcttcagt cggtgtccta ttcggagatt caggtatttt gagcaaacgt tcagttgata
     7201 acttaagcgt tggcttccat gaagaccatg gagttggtgc accgaagtct tatgtgattg
     7261 agtattatgt tggtaagact gtcccaacag ctcctaaaaa ccctagtttt gttggtaatg
     7321 aggaccatgt ctttaatgat tctgccaact ggaaaccagt tactaatcta aaagcccctg
     7381 ctcaactcaa ggctggagaa atgaaccact ttagctttga taaagttgaa acctatgctg
     7441 ttcgtattcg catggtgaga gcagacaaca aactaggaac gtctatcaca gaggtacaaa
     7501 tctttgcgaa acaagttgcg gcagccaagc aaggacaaac aagaatccaa gttgacggta
     7561 aagacttagc aaacttcaac cctgatttga cagactacta ccttgagtct gtagatggaa
     7621 aagttacggc agtcacagca agtgttagca acaatggcct cgctaccgtc gttccaagcg
     7681 ttcgtgaagg tgagccggtt cgtgtcatcg cgaaagctga aaatggcgac atcttaggag
     7741 aataccgtct gcacttcact aaggataaga acttactttc tcataaacca gttgctgcgg
     7801 ttaaacaagc tcgcttgcta caagtaggtc aagcacttga attgccgact aaggttccag
     7861 tttacttcac aggtaaagac ggctacgaaa caaaaaacct gacagttgaa tgggaagaag
     7921 ttccagcgga aaatctgata aaagcaggtc aatttaccgt tcgaggccat gtccttggta
     7981 gtgatcttgt tgctgaggtc actgtacgag tgacagacaa actaggcgaa gctctttcag
     8041 ataaccctaa ctatgatgaa aacagtaacc aggcctttgc ttcagcaacc aatgatattg
     8101 acaaaaactc tcatgaccgc gttgactatc tcaatgacgg agatcattca gaaaatcgtc
     8161 gttggacaaa ctggtctcca acaccatctt ctaatccaga agtatcagcg ggtgtgatct
     8221 tccgtgaaaa tggtaagatt gtagaacgga ctgttgctca agccaaactt cacttctttg
     8281 cagatagtgg tacggatgca ccaactaaac tcgttttaga acgctatgtc ggtccagagt
     8341 ttgaagtgcc aacctactat tcaaactacc aagcctacga cgcagaccat ccattcaaca
     8401 atccagaaaa ttgggaagct gtgccttatc gtgcggataa agacatcgca gctggtgatg
     8461 aaatcaacgt aacatttaaa gctgtcaaag ccaaagctat gagatggcgt atggagcgta
     8521 aagcagataa gagcggtgtt gcgatgattg agatgacctt ccttgcacca agtgaattgc
     8581 ctcaagaaag cacgcaatcg aagattcttg tagatggaaa agaacttgct gatttcgctg
     8641 aaaatcgtca agactatcaa attacctata aaggtcaacg gccaaaagtc tcagttgaag
     8701 aaaacaatca agtagcttca actgtggtag atagtggaga agatagcctt ccagtacttg
     8761 ttcgcctcgt ttcagaaagt ggaaaacaag tcaaggaata ccgtatccag ttgactaagg
     8821 aaaaaccagt ttctgagaag acagttgctg ctgtacaaga agatcttcca aaactcgaat
     8881 ttgttgaaaa agatttggcc tacaagacag ttgagaaaaa agattcaaca ctgtatctag
     8941 gtgaaactcg tgtagaacaa gaaggaaaaa ctggtaaaga acgtatcttt acagcgatta
     9001 atcctgatgg aagtaaggaa gaaaaactcc gtgaagtggt agaagctccg acagaccgca
     9061 tcgtcttggt tggaaccaaa ccagtagctc aagaagctaa aaaaccacaa gtgtcagaaa
     9121 aagcagatac aaaaccaatt gattcaagtg aagctagtca aactaataaa gcccagttac
     9181 caaatacagg tagtgcggca agccaagcag cagtagcagc aggtttagct cttctaggtt
     9241 tgagtgcagg attagtagtt actaaaggta aaaaagaaga ctagaaattt tgataccttc
     9301 tttatcatta aagaccaaag aaaaactctt gtcctaaata gaagataaag agtctggaac
     9361 aaaatagttc tcaaccacaa aaaagctaga tatttccaat tgcggaactc tagcttttta
     9421 attttgagtc gttctcttat tgtatagtag attgaaacta gagtagtaca cttctacttc
     9481 taaaacattg ttagaaatcg atttgactgt ccttatcgat ttgtcctgtt cttatttcat
     9541 ttcactatgt tttaggaggt tattggccat aagtaccaat cccatgtcaa ttctcacttg
     9601 acgcttgcct ctcagattac atctcttgta acccaaacaa gcctttatct gcccaaagac
     9661 aggttctaca tcaatcttgc gttgagcgaa aatctgtcta tcttggggag ataaaagcgc
     9721 ctgacattct ttagctttca agttttgata gcgttcgttc atatacaatc ccttttgagg
     9781 ggctgattca ggttcatcag cgtagtaaac cttgatttcc tgttgaaagt ctgtctgtgt
     9841 tttctggtac ttgatatggt gaaaacgata gcaccagcca tcaggatgta tgtagctatc
     9901 ctccttgtca ttatagtgcc aattcgctaa gtttttagct gactgtttat accctctctt
     9961 ttgttcctta tcaaacatgg catatttaat cagaagggga gtgatttctt cttgaaaata
    10021 gacttgtatc tgttcttgaa gtttggcgga gaatttctct gtcgttttct tccacacaaa
    10081 actatacttg ttggcattgg attcaatctt agttccgtca ataaacaaac aatctaaggt
    10141 cactaactct tccatttttc aatttttcga ccagagaaaa tcccttgtga ataggcaaat
    10201 agaagagtag atacaagcat tttagggtga taagacgggc gaccaaaggc atgataggag
    10261 gtgtagaagt gacgttcctc caaggtattc accacttttt caatagtaaa gacgagatgg
    10321 tcttgtggta agaaggaact gatttctagt ggtaaagttg tttgatttgt gttatagtga
    10381 atatgcatgg gatagccttt ctaaatggtt tattaagcaa ttctatttta ccaagactat
    10441 cgtaaccatg caaaaaagca acggcaaatc cgttgccttt tttggatata agagactatt
    10501 gtcccagact cttttctttg tataaaattg tatagtgtta ttgtctatac tattttgtag
    10561 agaaagcaaa aatccctaga aaatgcttag ggattcgact atttcattaa gaacaattca
    10621 acaatcatct ctatatacat gataagggtt aataaaaatc ctgtacgcca gaagaatttg
    10681 atgaatttag gatagtaaaa gctgcgtttt tttaaaagga agaaaaagac gagaataatg
    10741 gctaggaacg agagggctag ccccagtcta gggaggaaat tatgggtaaa ggttttagct
    10801 gtaatcaagt aatactcaaa taccaagact ggaaaagcca aatccgcaaa gtttcgtcct
    10861 agttttttta atctaaaaag tttggttagg ataatgcaga cgactaaggt cagtatcaat
    10921 aataaaatag atgctaattt cattaaaatc atacccatat tgtatcataa aaaaaggcta
    10981 atggaaacga gaaacagagg aatttatagg aaagtcaggc ttttgagatt gtgggtgatt
    11041 tttgttataa tgaaagttat aaaatcttat gataccttga atgattatta tcgaaaactc
    11101 tttggagaaa agacttttaa agtccctatt gatgcgggat ttgactgtcc caatcgtgat
    11161 ggaactgtgg ctcatggagg ctgtactttt tgtacggttt ccggttctgg agatgccatt
    11221 gtagccccag atccgcctat ccgtgagcaa ttttataagg aaattgactt tattcatcgt
    11281 aagtggccag atgttcagaa gtatctggtt tattttcaaa attttaccaa cacccatgaa
    11341 aaggtggaag ttattcgaga gcgttatgaa caggctatca atgagccagg tgtggtagga
    11401 atcaatattg gaacgcgccc agactgttta ccagacgaaa ccatcgaata tttggctgag
    11461 ttatcggagt gcatgcatgt gacggttgaa ttgggcttgc agactactta tgaagcaacc
    11521 tctgacctga ttaaccgtgc gcattcttat gaattgtagg tggaaacggt caagcgtttg
    11581 agaaagtatc caaaaattga gattgtttcc catttgatca atggtttgcc tggtgaaacc
    11641 catgagatga tggttgaaaa tgtccgccgt tgtgtcacgg ataacgatat tcaagggatt
    11701 aaactgcatt tgctccatct tatgacaaat actcgtatgc aacgagatta ccatgaggga
    11761 cgtttacaat tgatgagtca ggacgaatat gtcagggtca tctgtgacca actggaaatt
    11821 attcccaaac atatcgtcat ccatcgaatt acaggagatg cgcctaggga tatgctgatt
    11881 ggtcctatgt ggagcctcaa taaatgggaa gtcctcaaca gcattgagat ggagatgaga
    11941 cgtcgtggaa gtgttcaagg atgcaaggct gtaaaacagg agtttgaaaa tgaaaagacc
    12001 acttgagatg gcacatgatt ttttggctga ggtcgtgaca aaagaggatg tcgtagtgga
    12061 tgcgactatg ggaaatggtc atgacacgct ttttttagcc aagctagcca agcaagtcta
    12121 tgcctttgat attcagaagc aagccttgga aaagacccaa gagcgtttgc atcaggctgg
    12181 cttgacaaat gcccagttaa tcttgcaagg ccatgagaca ctggaccagt ttgtgacaaa
    12241 agctaaggca gggattttta atttgggcta tttgccgtca gctgataagt ctgtcatcac
    12301 ccgaccgcag acaacgattg aggcattaga aaagctatgt ggcttacttg tcaaaggtgg
    12361 acgaattgct attatgattt actatggtca tgaaggaggc gacctcgaga gagatgctgt
    12421 cttggatttt gtgatccagt tgaaccaaca agagtacaca gctgccattt accgaacttt
    12481 aaaccaagtc aacaacccgc cgtttttagt gatgattgaa aaattagaga gatacagaca
    12541 tggataaaca atacctacac gaaaagctgg atgccatgcg ccagaatttt gttgaatcaa
    12601 cctaccacga acgagcggtg ggcgtgctag accaagcgca tatgagcaaa aaaatgctta
    12661 aaatcaagaa aaaattagtt gctcttgaaa tggaacggtg ccagagaaaa attgagcaca
    12721 aagactgttc caagattgac caaaaaatca aagagcagaa ggagatattt gaatcctgtt
    12781 gtaaaaaaga ttaaggagga cgtgcgtgga attactgatt tacctcatcc tatttttact
    12841 ggtcttgatt gtctcaagta caaccaataa gctcctaccc tttttgcctc tccctttggt
    12901 gcagattctt ttgggaattg tgattggtct ctttttaccc aatactgact ttcaccttaa
    12961 tacggagttg tttttggcac tggttatcgg ccccttgctt ttccgagagg ctgaagaagc
    13021 agatgttacg gctattttaa aacactggcg aatcattgtt tatctcatat ttccagtgat
    13081 ttttatctcg accctgagtt tgggtggctt ggcccatctt ctttggttca gccttccctt
    13141 ggcagcttgc ttggctgttg gggcagccct tggtcctacg gacttggtgg cctttgcctc
    13201 tctttcggag cgttttagct ttcctaagcg cgtgtccaat attcttaagg gcgaaggact
    13261 cttgaatgat gcttctggtt tggtggcttt tcaggtagct ttgacagctt ggacaactgg
    13321 agctttttct ctggggcaag ctagcagttc actcatcttt tcaatcctag gcggtttttt
    13381 aattggattt ttaacagcta tgaccaaccg cttcctccat accttcttgc taagtgtgcg
    13441 cgcaacggat attgccagtg aacttttatt agagttgagt ttgcctctag tgaccttctt
    13501 tctggcagaa gaagtccatg tttcagggat tattgccgtc gtagttgctg gaattttaaa
    13561 ggcaagtcgc ttcaagaaaa tcacgctcct cgaagcccaa gtggatacgg tgaccgagac
    13621 ggtctggcat acagtgacct ttatgctcaa cggttctgtc tttgtgattt tagggatgga
    13681 gttggaaatg atagcagaac ctatcttgac caatccaatc tataatcctc tacttttatt
    13741 gctatctctc atcgccctta cctttgtcct ctttgtcatt cgttttatta tgatctatgg
    13801 ctattatgcc tatagaaccc gacgcctcaa gaaaaagcta aataagtata tgaaggacat
    13861 gtttctcttg accttttcag gtgttaaggg aacggtgtcg attgctacga ttctcttgat
    13921 accaagtaat ctagaacagg agtatcctct cttgcttttc cttgttgcag gtgtgacgct
    13981 tgtcagcttt ttaacaggtc tcttggtctt gcctcatctt tctgatgaag aggaagaaag
    14041 caaggattat ctcatgcata tcgccatttt gaatgaagta acgctagagt tggaaaaaga
    14101 gttggaagac accagaaata aacttcccct ctatgcggct attgacaatt atcatggacg
    14161 tattgaaaat ctcattttaa gccaagaaaa ccaggatgat caagaagact gggctgcttt
    14221 gaaactcttg attcttagta ttgaaagtga tggtttggaa caggcctatg aagaggggaa
    14281 cattagcaat cgtgcttacc gagtttacca acgttatctg aaaaatatag aacaaggaat
    14341 caatcgtaaa cttgcctcaa gactgaccta ttattttctt gtttccttga ggattttacg
    14401 ttttctcctt catgaagttt ttactcttgg aaagaccttc cgtagctgga aggacaagga
    14461 gcaaagccgt ctccgtgctc ttgattatga ccaaattgca gagctctatc ttgccaatac
    14521 agagatgatt attgaaagtt tggaaaacct gaagggagtc tacagacgct ctttgattag
    14581 ttttatgcag gagtctcgtc ttcgagaaac agctattatc agcagtggtg cctttgtcga
    14641 acgggttatc aatcgtgtca aacccaacaa tatcgatgaa atgctgagag gctattatct
    14701 ggagcgcaag ttgattttcg aatacgaaga aaaacgattg attacgacta agtatgccaa
    14761 gaaattacga caaaatgtaa ataacttaga gaactattcc ttgaaggaag ctgccaatac
    14821 cctaccgtat gatatggtgg aattggtaag aagaaattag ttaatactct tcgaaaatct
    14881 cttcaaacca cgtcagcgtc gccttaccgt atgtatgtga ctgacttcgt cagtcttatc
    14941 tacaacctca aaacagtgtt ttgagcagct tgcggctagc tttctagttt gctctttgat
    15001 tttcattgag tataagatta taagtgaagg agtatgacat gaaaaagtgg tggaaagagc
    15061 tgatagacaa acctttatta aaagcttttt tgcattatta tcaagcatca gatagtgagt
    15121 tgaccagtgt cgcagtagcc tactattggt tgatttcgat tttccccctg cttttggtgg
    15181 tggtcaatat cctcccctat tttcagattc ctgtgggaga atttctgggc tttatgaaag
    15241 acgtcctgcc cccaagcctc tatgaaggtg tggaaaaaat agccagagaa gtcttgacgc
    15301 aaccttcaac aggtttattg agtttttctg ttttatcggc gttatggagt ttttcaaaat
    15361 ctatgaattt cttgcaaaaa gcttttaaca aggcttatgg cgtcgaaaag agtcgtggac
    15421 ttatttccca tcagatgcta agtctcctag tcagttttgg cttgcagctc ctctttgcgt
    15481 ttgccttgtt tttgatctta tttggtcaga tgattttggc tttacttgct cattattgga
    15541 caaaagatgg cattatctat caggctttac aagggttggc aggtcctctg atttacgccc
    15601 tcctctttgc tatcttggtc atgctttatt attttcttcc caatctatct aacaggaaaa
    15661 ttagctatac cctaccaggc agtgcctttg ttctcttagt gattttaggt ttactaactc
    15721 tgttctctag ttatctcaat tactatgtcc atcatttggt ggatgtccga attttaggtt
    15781 ccgttcttct tgttgttatg atgttttggt ttatcttgat tgctaaaatt gttatcctag
    15841 gcgcggttat caatgccagt atgcagagtt tgaaagatcc ggtctttaaa aaagactaac
    15901 tatttatcca ttataaaagc gcatagtatc aagactttat agaaccttga tactatgcgc
    15961 ttttgtgctt tgacagtttt ttgtatagtt tttgtccaag atttctaaat catctgatta
    16021 aattcctatt ttcccctatc attgtccctg ttttttcgaa aatggaagga gtataataaa
    16081 tctatcaata aaatgatagg aggaacttta tgggtcatat tttctttttt ctaagtgtct
    16141 ttttggcagg gattctatcc ttcttttctc cttgtatctt acctttgtta ccggtctata
    16201 caggagtgtt actagatgat aaggatggtg ctcaggcttc tagcggcaaa ttttcaatct
    16261 cagttactag tttattacga actctggcct ttatagcagg aatttccttt atatttattt
    16321 tgttgggcta tggagctggt tttttaggcg atttgcttta tgcttcttgg ttccaatatc
    16381 ttactggggc aattattatc cttcttggtt tgcaccaaat ggagattcta cactttaagg
    16441 ggctttataa ggaaaagaga ctacaactgc aaggacaggg gcaaaatggt aagggctata
    16501 gtcaggcatt tttattgggc ttgaccttta gttttgcttg gacgccttgc gtggggccgg
    16561 ttctggggtc tgttttggcc ttggcggctt caggtggttc aggagcttgg cagggagctg
    16621 gtctcatgtt ggtgtatacg ctgggcttgg cgctaccatt cttgcttcta gctctgacct
    16681 ctagttatgt tttgaaacat ttccgaaaac ttcatcccta tctcggaatc ctcaaaaaag
    16741 tgggtggttt tctcattatt gtgatgggac tcttggttct gtttggaaat gcttcaattt
    16801 taagtcaatt atttgaataa aatggaaagg aatatcaata tgaaaaaatg gcaaacatgt
    16861 gttcttggag caggttcgct cctttgtttg acggcttgtt caggcaagtc cgtgactagt
    16921 gaacaccaaa cgaaagatga aatgaagacg gagcagacag ctagtaaaac aagcgcagct
    16981 aaagggaaag aggtggctga ttttgaattg atgggagtag atggcaagac ctaccgttta
    17041 tctgattaca agggcaagaa agtctatctc aaattctggg cttcttggtg ttccatctgt
    17101 ctggctagtc ttccagatac ggatgagatt gctaaagaag ctggtgatga ctatgtggtc
    17161 ttgacagtag tgtcaccagg acataaggga gagcaatctg aagcggactt taagaattgg
    17221 tataagggat tggattataa aaatctccca gtcctagttg acccatcagg caaacttttg
    17281 gaaacttatg gtgtccgttc ttacccaacc caagccttta tagacaaaga aggcaagctg
    17341 gtcaaaacac atccaggatt catggaaaaa gatgcaattt tgcaaacttt gaaggaatta
    17401 gcctaggagg tgtcttatga atgataagtt aaaaatcttc ttgttgctag gagtattttt
    17461 tctagccata accggtttct atgttctatt gatacgaaat gcagggcaga cagatgcctc
    17521 gcaaattgaa aaggcggcag ttagccaagg aggaaaagca gtgaaaaaaa cagaaattag
    17581 taaagacgca gacttgcacg aaatttatct agctggaggt tgtttctggg gagtggagga
    17641 atatttctca cgcgttcccg gggtgacgga tgccgtttca ggctatgcaa atggtagagg
    17701 agaaacaacc aagtacgaat tgattaacca aacaggtcat gcagaaaccg tccatgtcac
    17761 ctatgatgcc aagcaaattt ctctcaagga aatcatgctt cactatttcc gcattatcaa
    17821 tccaaccagc aaaaataaac aaggaaatga tgtggggacc cagtaccgta ctggtgttta
    17881 ttacacagat gacaaggatt tggaagtgat taaccaagtc tttgatgagg tggctaagaa
    17941 atacgatcaa cctctagcag ttgaaaagga aaacttgaag aattttgtgg tggctgagga
    18001 ttaccatcaa gactatctca agaaaaatcc aaatggctac tgccatatca atgttaatca
    18061 ggcggcctat cctgtcattg atgccagcaa atatccaaaa ccaagtgatg aggaattgaa
    18121 aaagaccctg tcacctgagg agtatgcagt tacccaggaa aatcaaacag aacgagcttt
    18181 ctcaaaccgt tactgggata aatttgaatc cggtatctat gtggatatag caactgggga
    18241 acctctcttt tcatcaaaag acaaatttga gtctggttgt ggctggccta gttttaccca
    18301 acccatcagt ccagatgttg tcacctacaa ggaagataag tcctacaata tgacgcgtat
    18361 ggaagtgcgg agccgagtag gagattctca ccttgggcat gtctttacgg atggtccaca
    18421 ggacaagggc ggcttacgtt actgtatcaa tagcctctct atccgcttta ttcccaaaga
    18481 ccaaatggaa gaaaaaggct acgcttattt actagattat gttgattaat actcttcgaa
    18541 aatctcttca aaccacgtca gcgtcgcctt accgtatgta gggttactga cttcgtcagt
    18601 tttatctaca acctcaaagc tatactttga gctgacttcg tcagttttat ttacaacctc
    18661 aaagctatac tttgagaaac ctgtggctat cttcctagtt tactttttga tttttattga
    18721 gtataagagg tctttcctaa gcagttagag gagaattttg ctatactgat actagtaagt
    18781 gacaaaggag agaagcatga cctacacaat cttaatcgta gaagatgaat atctggtaag
    18841 acaaggtttg actaaactgg tcaatgtagc agcctacgat atggaaatca tcggtcaggc
    18901 tgaaaatgga aggcaggctt gggaattgat ccaaaagcag gtgccagata tcattttaac
    18961 cgatatcaac atgcctcatc taaatggcat ccagttggcc agtctggtac gagaaaccta
    19021 tcctcaggtt catttggtct ttttaacagg ttacgatgat tttgattatg ccttgtctgc
    19081 tgtcaaacta ggtgtggacg actacctgct caaacccttt tctcgtcagg atattgagga
    19141 aatgttgggg aaaatcaaac aaaaactaga caaggaagag aaagaagagc agttacaaga
    19201 tttattgact aataggtttg aaggaaacat ggcccagaaa atccagtctc atctggctga
    19261 tagccaattt agtttaaagt ctttagccag tgacttaggt tttagtccga cctatctgag
    19321 ttccttgatt aagaaagagt tgggcttgcc ttttcaggat tatctggtga gagaacgtgt
    19381 taaacaagcc aagctcttgc ttttaactac agatctgaag atttatgaga tcgcagagaa
    19441 ggttggtttt gaagatatga actattttac ccaacgtttt aagcagattg caggtgtgac
    19501 acctcgtcag tttaagaagg gagaagaccg atgaagcgtt cttctctttt agttagaatg
    19561 gttatttcca tctttctggt ctttctcatt ctcctagctc tggttggaac tttctactat
    19621 caatcaagtt cttcagccat tgaggccacc attgagggca acagccaaac gaccatcagc
    19681 cagactagcc actttattca gtcttatatc aaaaaactag aaaccacctc gactggtttg
    19741 acccagcaga cggatgttct ggcctatgct gagaatccca gtcaagacaa ggtcgaggga
    19801 atccgagatt tgtttttgac catcttgaag tcagataagg acttgaaaac tgttgtgctg
    19861 gtgaccaaat ctggtcaggt catttctaca gatgacagtg tgcagatgaa aacttcctct
    19921 gatatgatgg ctgaggattg gtaccaaaag gctattcatc agggagctat gcctgttttg
    19981 actccagctc gtaaatcaga tagtcagtgg gtcatttctg tcactcaaga acttgttgat
    20041 gcaaagggag ccaatcttgg tgtgcttcgt ttggatattt cttatgaaac tctggaagcc
    20101 tatctcaatc aactccagtt ggggcagcag ggctttgcct tcattatcaa tgaaaaccat
    20161 gaatttgtct accatcctca acacacagtt tatagttcgt ctagcaaaat ggaggctatg
    20221 aaaccctaca tcgagacagg tcagggttat actcctggtc acaaatccta cgtcagtcaa
    20281 gagaagattg caggaactga ttggacggta cttggcgtgt catcattgga aaagttagac
    20341 caggttcgga gtcagctctt gtggaccttg cttggggcca gtgtcacatc tcttcttgtc
    20401 tgtctctgct tagtgtggtt cagtcttaaa cgctggattg ctcctttgaa ggatttgaga
    20461 gaaaccatgt tggaaattgc ttctggtgct caaaatcttc gtgccaagga agttggtgcc
    20521 tatgaactga gagaagtaac tcgccaattt aatgccatgt tggatcagat tgatcagttg
    20581 atggtagcta ttcgtagcca ggaagaaacg acccgtcagt accaacttca agccctttcg
    20641 agccagatta atccacattt cctctataac actttggaca ccatcatctg gatggctgaa
    20701 tttcatgata gtcagcgagt ggtgcaggtg accaagtcct tggcaaccta tttccgcttg
    20761 gcgctcaatc aaggcaagga cttgatttgt ctctctgacg aaatcaatca tgtccgccag
    20821 tatctcttta tccagaaaca acgctatgga gataagctgg aatacgaaat taatgaaaat
    20881 gttgcctttg ataatttagt cttacccaag ctggtcctac aaccccttgt agaaaatgct
    20941 ctttaccatg gcattaagga aaaggaaggt cagggccata ttaaactttc tgtccagaaa
    21001 caggattcgg gattggtcat ccgtattgag gatgatggcg ttggcttcca agatgctggt
    21061 gatagtagtc aaagtcaact caaacgtggg ggagttggtc ttcaaaatgt cgatcaacgg
    21121 ctcaaacttc attttggagc caattaccag atgaagattg attctagacc ccaaaaagga
    21181 acgaaagttg aaatatatat aaatagaata gaaactagct aactcccagt caattctggg
    21241 agttttatgc gtaattgggc aaactttcta ggttgtctat cttgctaaaa acaagaaaaa
    21301 acggtatgat agaagagtga caacagaggt aacagagatg aacgagacac agattcaaag
    21361 agaaacacgt caggtagtgg aagatgtttt agaaaagacc aatttgaaac agggggccct
    21421 ttttgtttta ggtctatctt ctagtgaggt gctaggtggt cagattggga aagaatccag
    21481 tcaagagatt ggtgagctga tagtggagac tatcttgggt attctaggta gtagaggtat
    21541 ccatttagca gttcaaggtt gtgagcatgt caatcgggct ctcgttgttg aacgtcaggt
    21601 ggcagagcag tttggtctgg aaattgtcag tgtccatcct actcttcatg caggaggttc
    21661 ggggcagttg gcagccttta agtttatgca ggatccagtt gaggttgaat ttatcaaggc
    21721 tcatgctgga ttggatatcg gagacactgc aattggcatg catgtcaagc atgttcaggt
    21781 tccgattcgc cctattttga gagagattgg tcatgcccat gtaacggcac tggctagtcg
    21841 tccaaaatta atcggaggtg cgcgtgcgca ctatccgcaa gatgctatta gaaagacctg
    21901 agaatgaata aaggagataa aagtgtttaa aaaagaccgt ttttcaattc gtaagattaa
    21961 gggagttgta ggctctgtat ttcttggaag ccttttgatg gctccttctg tagtggacgc
    22021 agccacctat cactatgtaa ataaagagat tatttcacaa gaagctaaag atttaattca
    22081 gacaggaaag cctgacagga atgaagttgt atatggtttg gtgtatcaaa aagatcagtt
    22141 gcctcaaaca gggacagaag catctgtttt gacagctttt ggttatctat caggtgacat
    22201 cctcaaaacg cttggcttgg acactgtttt agaagaaacc tcagcaaaac ctggagaggt
    22261 gactgtagtc gaagttgaga ctcctcaatc aacaacaaat caggagcaag ctaggacaga
    22321 aaaccaagta gtagagacag aggaagctcc aaaagaagaa gcacctaaaa cagaagaaag
    22381 tccaaaggaa gaaccaaaat cggagataaa acctactgac gacacccttc ctaaagtaga
    22441 agaggggaaa gaagattcag cagaaccagc tccagttgaa gaagtaggtg gagaagttga
    22501 gtcaaaacca gaggaaaaag tagcagttaa gccagaaagt caaccatcag acaaaccaac
    22561 agaggaacca aaagttgaac aagtaggtga accagtcgag ccaagtgaag acgaacaggc
    22621 accaacggca ccagttgagc cagaaaagca accagaagct cctgaagaag agaaggctgt
    22681 agaggaaaca ccgaaaccag aagataaaat aaagggtatt ggtactaaag aaccagttga
    22741 taaaagtgag ttaaataatc aaattgataa agctagttca gtttctccta ctgattattc
    22801 tacagcaagt tacaatgatc ttggacctgt tttagaaact gcaaaaggtg tctatgcttc
    22861 agagcctgta aaacagcctg aggtaaatag tgagacaaat aaacttaaaa cggctattga
    22921 cgctctaaac gttgataaaa ctgaattaaa caatacgatt gcagatgcaa aaacaaaggt
    22981 aaaagaacat tacagtgata gaagttggca aaacctccaa actggggtta cagaagctga
    23041 aaaagttgca gctaatacag atgctaaaca aagtgaagta aattctgaga ctgcaagtct
    23101 taaaacagca atttctagat taaatacaga taaagttgaa ctcgaaaatc aacttaagat
    23161 tgcacaggga aaaactgaaa ctgatttcag catggaatct tggactgttt tgagtacagc
    23221 taaaaataaa gctcaggaag taaaagataa cggaacagcg acccaagaac aaataaacga
    23281 agcagagaaa agtttgaaaa cagcccttgc tgacttatct gttgacaaaa cagctttggg
    23341 aagcgctatc gatacagcta ctaagaaaaa taaagagaat tatacaaatc aaacatgggc
    23401 agagttggaa acagcgctta cagcagcaaa atctgtaaat actaacgagt ctaaacaaag
    23461 cgacgttaac gaagctgctg aaaaattaac tgcaactatg gaaaaattgg ttgaattgtc
    23521 tgaaaagcca cgtttgactc tatctattga aaagagagac atagatcgaa aagctacggt
    23581 cacgtatact ttggaaaatc cagcaaatac tcaaataaaa tcaataactg caactctgaa
    23641 aaaaggagag gaagttgtta aggattttgt tttaacagaa gaaaatctta aaactaatca
    23701 tttaactgct ttgtttgaaa agctagacta ctacaaagaa tacactctat ctacggatat
    23761 ggtatataac agaggaaatg atgatgagac ggagagtatt tcagaagaac taattcagct
    23821 gaatcttaag aaactagaat tgaaagatat ccagacagtc tctctgatga aatttgaaaa
    23881 tggtcaagaa agtcaggtta cacacttgtc tgataaaccg accgatttga gtaaattata
    23941 cttaaaagtt acatcatcaa cgtctaagga tgctgtgtta gccgttagct ctatagaaga
    24001 agagattatt gaaaataaaa aaatctttaa aattcatgca gatacaccag aattagtagt
    24061 tcgtaagaaa gatggcagtc tttctaaggg atttgactat tacatggaga gagttatccc
    24121 tcatgatggt gatatttatt atgattttaa agacttgatt tcagctatga catctaatcc
    24181 tacaggtacg tttattttag ggagagatat ttcctcgcgt aatgtaaaac cggatggaaa
    24241 tggaaaatct tatatcaaag gtgaatttaa aggaaaacta cttggtacaa atgataatgt
    24301 tagacactcg atctttgact tagaatatcc attgtttgat actataaaat caggagtcgt
    24361 gaaagatatt gatttcaagc atgtcaatat ggtattccca gatagcaatc aaggagataa
    24421 cgtagcaaca attgcaagag taatcaaaga taagacaaaa attgagaatg ttaatgttga
    24481 aggttatctc gagggtcgag atcatgtagc tggtttagtt aataatcttg aaggtaattc
    24541 cgaaattgaa aatgtatcct ttacaggaaa gattaagtct aaaggtggta attctattac
    24601 agcaggtatt gcaggtagaa atatcttatc tcgtgttaaa cgtgcctatg taaatgcaga
    24661 tatagaggta catcgatcat ctaattctag catgcttgtt gcagtaaatg gaataaacgc
    24721 agatgcaagt ggtggttggg gaacctgggg tagacttact gagtcagttg ctaaaggaac
    24781 attagaaacc aagcaaggag ggcaagctgg aggagcctct tctacagtct ggccttatgg
    24841 agcaattgat aatgttgtca gctatgcaaa agtgaccaaa gggaaagaat tatttggatc
    24901 agatggtgat ttaaattatg attggtttat gaaaaaaatt agtaatattt ttggtgttca
    24961 aggcatcagc tcaggcgatt cgggtagtga ttctaaattt acgcgtattt ctgaggaaga
    25021 agcaaatcaa aaagttgctt cttataatat tacagctccc aacttgatga gtgactcaag
    25081 tcttttggta gacagactca atgaaagttg gaaaaatact gatcaatttg aaagtataca
    25141 ggattatcaa gcccaaaacc aattaattta ccagaacttg actaaattta ctccttatta
    25201 caataaagaa tttattgttc atgaaggaaa tgctctcact cctgaacagg aaatattgaa
    25261 gactaagaaa atcaaatcga ttgtagggtt gaaaggaaca gagttcgttg tagatggcag
    25321 tgatattgat actatcatgc ttcattttga ggatggtagt cagaagcgat ataaggtaac
    25381 ctctactggt aagttttcga taactaattt accggaatat caggttgaag atttaaatgt
    25441 ggtgtataca tcagaacata tagtacatcc actagattcg tcactgataa ataaccttgt
    25501 tgaagaattg aaaaaagtag aattgtatac agagtctact taccaagttc taggtattga
    25561 taaagataat gctaacaaat tgaatagaac taagagatta ttcttagatg agtcattaga
    25621 tgctgttaaa acacaattgc ctacctttgt aaaaacaatg tttgaaaatg agtggttgca
    25681 catcaatgga gaatctagtg gagctgtcgc tgctttacga caaaaaatca tggataataa
    25741 gacggctata ttgcttgctt tgacttatat caatcgttat tatgatgtta aattctcaga
    25801 ctataatatt aaaaaactga tgctctttaa accaactttc catggtgaaa agattgatct
    25861 attagatcga ttaatccgac taggatcatc tggagaaaat agattaaaag gttcagaaaa
    25921 tgcagaaacg ttcaagcagt tatttgctag cgaaacgaag cagaaggatt tggtgactta
    25981 tttagactac aatcgctctt tgttgactaa ttatcaaaca acaggggaat ggtttaaaga
    26041 aacgactaag gattatattc aatttgagga acgtccatcc ttagtagagg agattaaaga
    26101 tgccaaatat cgagtttacg ataatttaac cgcaccgtat tatcaaggat atatcctacc
    26161 tctcttaacg ttgaagaata ctcatttagc aattttatcg aactatagta ctatgacatt
    26221 tgtcagccgt gaaaaacgcc ctaattggaa aaatgaagat tttgataagt gggtgaaata
    26281 tgtagcaact gctcatagaa accatgttga tacttggtat aagattttac ctgataatat
    26341 caaaggtaaa atggttaaag agaatgttac tgctgtttgg gaaggattga gcatcccagg
    26401 aagtgaatgg gtagaccaga atgctgtaga tagaaaagga agagattacg ccccagctcg
    26461 agaattcttt aatcttgtag gtggtcctat gggaggttgg tatgcttacc atggttatgg
    26521 tgcgcatgct ggaggaagaa atcgggttaa ttatgaggtg tttgatgtat taagtgagta
    26581 tggtatatcg gtctttactc atgaattgac tcacgttaat gatacttgga tttacttagg
    26641 agggtatggt agacgtgaaa atatgggacc tgaagcttat gctcagggat tattccagtc
    26701 tcctgttcca ggtcagccgg gttggggggc gcttggtctc aatatggcat ttgaaagaaa
    26761 aaatgatggt gatctaattt ataatgcctc cccaactcaa tttgaaaata ggaaagagtt
    26821 agatagttat atgaaaaatt ataacgatac tctcatgatg gtggactact tggaaggaga
    26881 tgcagttatc tctaaaggta aagaagctat tactaaatgg tttaaaaaag ttgaacctaa
    26941 ggttgttagt caaacagctc agtatgatac ggttcgtcag ttgactgctg aagagaaaga
    27001 aaaactttct gtttctagtg ttgatgattt agttgatcaa ggattaatgt cagacagagc
    27061 tgtaggaaat aatacctata atccagctga ttttgaaaca agttatattg cgatagatta
    27121 tatgactggt atttatggtg gaggaaagaa tagtgtaggt tcaccaggtg ccttgatgtt
    27181 taaacataat actttccgta tgtggggcta ctatggtttt gaagaaggtg ttctaggata
    27241 tgcttctaat aaatttaagc aagcatctag agatgaaggt catgctggac ttagtgataa
    27301 ctttattatt tctaagattt ccaaagggga atttttaact atggaagcat ttaaaaaggg
    27361 atattttaag aaagtagtag aagagttaaa aactaaaggt attcgtcctg ttactatcaa
    27421 tcaaaaaaca tatagcacct ttgaagaatt gcaagaagga tttaagcagg ctgttgaaag
    27481 agatttgaag aaaaatcagt tagatgagag ggaaacaaga aacttcaaat tccaagtttt
    27541 cagacaactt cttcaacaaa cagatagctt taaaacttct atctttagat aatcgaaatc
    27601 tctcatctgc tttgcggatg agggttttct tattttatgc tatacttaaa gtatgcatag
    27661 aaaaacagtg attgatttta ggactttggg ggagagatat acctttaccc agcctattaa
    27721 agagttgaaa acgagaaatg tagaagaagt ggcagatttg ctggcacaag tggaaagcta
    27781 ccaagagcaa ggttattatg tggtggggta tgtcagctac gaggctgcac ctgcttttga
    27841 ggaaaaatta gcagttcaca aggatcccct actgggagag tacctgcttt attttactgt
    27901 tcatgatagc gtagaaactt cccctattcc tctgacttat gaggatattg atttgccctc
    27961 aaattggcag gaagtaacgt ctgcagcaga ctatgaaaag gccattgccc aaattcacca
    28021 tcatttacgg cagggagata cctatcaggt caactacacc gtccaactta agcaaaagtt
    28081 aaatgccaat ccttttgcca tctacaatcg tatggtggta gagcaggagg cgggctacaa
    28141 tgcctatgtg gaacatgacg agatggcagt gatttccatg agcccagagc tcttttttga
    28201 gcaaaatgat cgcgagttga caacacgacc aatgaagggg acgactcagc gtggggtaac
    28261 tgaccaagaa gatcttgaac aggccagttg gttggaacag gatcccaaaa atcgctctga
    28321 aaatatgatg attgtggacc tcttgcgcaa tgatatgaac cgtatttctg aagttgggag
    28381 cgagcacgtg gagcgtctgt gtcaggtaga gcagtattca actgtttggc agatgacttc
    28441 gaccatcaag agtcagttgc gagaggatgt ggaccttgtt gaaatcttcc actcactctt
    28501 tccttgtggt tccataacgg gtgcaccgaa aattgcgaca atggagatta tcaaggactt
    28561 ggagcctcaa ccgcgtggag tctactgtgg aacgattggt ctcttgcttc caaatggacg
    28621 acggattttt aatgtggcca ttcgtaccat tcaacttcac aaaggtcaag ccatttatgg
    28681 agttggcgga gggattactt gggatagcac ttgggaatct gaataccgtg aagttcatca
    28741 aaaggcagca gttctctatc gtaaacaagc tcgcttccaa ttaattacaa ctggaaaaat
    28801 cagccaaaaa caactgatgt ttgaagaaca acatctggaa aggctgagaa aagctagtcg
    28861 gtattttgct tatccttttg acccagaaat tttgagacaa aagattgaag cagagtgtca
    28921 gacttgtgat gctaatcaag attaccgctt gcgaatttct ctcagcaagt ctggagagat
    28981 agaattcagt cgtcaaatct taacacctct tagtccaagt ttttgtcagg ccaaactttg
    29041 tctgcaagaa gcgaatttga atcaatcctt tacctatttt aaaacgactt acagaccgca
    29101 tttgagcata gaggaacaag agaagattta ccataatgga aaaggagaac ttcttgaaac
    29161 ctctatcgga aatttggttc tgaaaatcga tgggaaactc tacacaccgc ctatccgact
    29221 tggaatcttg ccaggaattt atcaccagta tttactagaa acaggacagg tagaagagaa
    29281 agtcttgacc ttggcagatt ttgcccaagc agaagctatt tacggctgta atgcagtgag
    29341 aggcttgtat gagttgcaag tgataggatt gtgagtttta gctattaata gtttttaata
    29401 tatattaaaa tatattggtg tttacttttg atgagatatc atatttgtgt tataatgtaa
    29461 ccgtaaacaa tagaaattta aaggagattt ttgatgaaat caaaaatagt tttgggagct
    29521 tcgctagcga tcgcaacact tagcttagta tctcttgtcg agattgaagg actctcaccg
    29581 tttttaatag agaatgttag cgcaaatact cattctgcaa ataaagttat taatcataaa
    29641 gtaagtattt acttagaaaa tgctgatgaa ggtaaaggtc taactgtaaa cttttcaaca
    29701 gacagtgtta gtccaaattt atttgatgag ttcgaaaaaa agagtggaat tacaataact
    29761 actatgttag tgaatgctaa gacaggtgaa gttgtagaaa aaagattaac accaagcgtt
    29821 tttttacgtt ccaatgattt aaccagtggg accatatctt cgtttatttt tagtgaatat
    29881 cctgatggag aatataaata tgttgtgtct aaaggagact ttatcgatcc taagactcag
    29941 tttaaacatc agtatagagg agaatctcca gtttttagaa tacgtaatcg aaaatatgtg
    30001 gagttgggaa caactgataa aaaattagat gaaagacgtg ataatagcgt ttataaagat
    30061 ggggttgttg aacacaaggt aaatttaagt ttaacaagtt atcaaggagg taatggagtt
    30121 actgcaattt tttcaactga tagtgttaac tctaacttac ttaattcttt tggtgaaaag
    30181 gctaaaaaag ttttgattag gtctaagtta attaatgtta agactggcga ggttattgat
    30241 gaaacgtttt ctccaaaagt aagtttaacc tctaaaattt tgaaaagtgg ttctacagca
    30301 gtattttatt ttatagattt aactgatggc gaatataaat atgttgcgta tgaaagccag
    30361 caatatactg atcctcaaac aacattgact catcagtata gaggagaatc tcctattttt
    30421 agtatcaaag atggaaaatt ttctggttta gtatctgcct caaaaccaga cgaaaacccg
    30481 aagccaacgc caaaaccgga tgagaaacca aaaccttcag caccacaaca ggagaaaaca
    30541 aaacctacag ttcagtctgg ttgggtaggt tcatcttatt atcaaaatgg taaaaaagtc
    30601 actagtaagt ggatttttga taaaaaatat aattcatact tctatctaga tgcatcagga
    30661 aattatgttc aaaacgcttg ggtaggtaac tattatctca aatcaggcgg atacatggct
    30721 aaaagtgagt ggatttatga taagaactat ggttcatact actatctaac atcagaaggc
    30781 agctacgctc gtaacacttg gtcaggcaat tattatttga agtcaaatgg taagatggct
    30841 aaaagtgagt gggtatacga tagtaactac aaatcatatt actacttgac atcagaagga
    30901 agttatgctc gtaacacttg ggtaggcaat tactatctta aatcaaacgg taaaatggct
    30961 gttaatgaac gtacaccaga tggttatcgt gtagatggtt caggtaaatg ggttaaataa
    31021 gaaactaaaa aataggataa taggtaatat cagatgaata aaaaaaatta ttttcatgtg
    31081 ctttagcatt ttcaacgatt ggaacattgt caacgattga taaatcagga gttcttcctt
    31141 tttcagcgca aacggtgcag gctgacatac aaacgcttac cccaatggca aaatattata
    31201 ttattcttcc gaaggatgca gaaatatata aaacttggag aggtactgtc aatattccca
    31261 ttattgatgc tacaaaaact actccagaat tatcttattt caaagaagat catcgcaact
    31321 atatagctaa tgagaataag tctggtgcca attacataga gtggaaggga acagtagaag
    31381 agtttaaaga ggccattaag aagttgactg ataaaaaatc aactactgca acaccaaaaa
    31441 aggatgaaaa gccaacacca aaaccggatg agaagccaaa accaacacct acagttcagt
    31501 ctggttgggt aggttcatcc tattatcaag atggtaaaaa agtcattagt aagtggattt
    31561 ttgataaaaa atataattca tacttctatc tagatgcatc aggaaattat gttcaaaacg
    31621 cttgggtagg taactattat cttaaatcag gcggatacat ggctaaaggt gaatgggttt
    31681 atgatgccac ctatcaagct tggtattatt tgacatcaga tggttcttat gcttacagta
    31741 catggcaagg aaattactat ctaaaatcgg atggtaaaat ggctgtcaat gaatgggttg
    31801 atggtggacg ttattatgtt ggcgctgacg gagtttggaa ggaaggtcaa gcaagtacag
    31861 cttcttctag taatgatagc aatagtgaat attctgctgc tttaggaaag gcaaaaagtt
    31921 ataattcgtt attccacatg tcaaaaaaaa acgtatgtat agacaattaa cttctgattt
    31981 tgataaattt tcaaatgatg cagctcaata tgccattgat cattagatga ttaacagata
    32041 ctggtaggcg aaaaaattcc ttataattca cttacgcaaa cctttgcata agtgaatgga
    32101 ttttgatata ctagtactgt aagcattttc aattactaca caatgaaaaa ggagaatatg
    32161 atgagtcaaa agattattgg gattgacctt ggtggaactt ctatcaaatt tgcaatctta
    32221 acaacagcag gagaaatcca aggaaaatgg tcaatcaaga ccaacatttt ggatgaggga
    32281 agtcatatcg ttgatgatat gattgagtct attcaacatc gtttggactt gcttggattg
    32341 gcagcagcgg acttccaagg cattggaatg ggatcaccag gtgtggttga ccgtgaaaaa
    32401 gggactgtta tcggtgccta caacttgaac tggaaaaccc ttcaaccaat caaacaaaag
    32461 attgaaaaag ccttgggcat tccatttttc atcgataatg atgccaacgt agcagctctt
    32521 ggtgagcgct ggatgggtgc tggagataac caaccagacg ttgtctttat gacactcggt
    32581 actggtgttg gtggcggtat cgtcgcagaa ggcaaattgc ttcacggtgt tgctggtgca
    32641 gcaggtgagt tgggccacat cactgttgac tttgaccagc caattgcatg tacttgcggt
    32701 aagaaaggct gccttgagac agttgcttca gcaacaggga ttgtcaactt gactcgtcgc
    32761 tatgccgatg aatacgaagg cgatgcagcc ttgaaacgct tgattgataa cggagaagaa
    32821 gtaactgcta agactgtctt tgagctcgca aaagaagaag acgaccttgc tttgattgtt
    32881 taccgtaact tctcacgtta cttgggtatt gcatgtgcta acattggttc aatcctaaac
    32941 ccatcaacaa tcgtcatcgg tggtggtgtg tcagctgcag gagaattcct tctacaaggt
    33001 gttcaaaaag tctacgatga aaatagtttc ccacaagtac gcacatctac taaattagca
    33061 cttgcaactc taggaaatga cgctggagtt atcggagcag catcacttgt attgcaataa
    33121 gataatgaga gaggaaaatg ctaacctaga gttagtaatg ttcctctttt attttttatg
    33181 ctatactagt taggacaatg aatgaggtga aacgaaatga caaaagcaga tacgattttt
    33241 aaagagaata ttgaacgcat tctaaaggaa ggtgttttct cagagcaggc tcgtcctaag
    33301 tacaaggatg ggactgttgc taattccaag tatgtaactg gtgccttttc cgagtacgac
    33361 ttatctaaag gagaatttcc tatcacaacc ttgcgtccca ttgcaatcaa atccgccatc
    33421 aaagaagttc tctggattta ccaagaccag tcaaatagct tagaagtgct taatgacaag
    33481 tacaatgttc actactggaa tgactgggaa gttggagaca cgggaaccat tggtgagcgc
    33541 tatggtgccg ttgttaagaa acacgacatt atcaataagc ttctcaaaca gttggaaacc
    33601 aatccttgga accgccgcaa tattatttcg ctctgggatt accaagcttt cgaagaaaca
    33661 gatgggctgc tcccgtgcgc ctttcagacc atgtttgatg tccggcgtgt tgatggggaa
    33721 atctatctgg atgcgacctt gacccagcgc tccaatgata tgctggtggc ccaccacatc
    33781 aacgctatgc agtatgtggc tttgcagatg atgattgcca aacattttgg ctggaaggtt
    33841 gggaagttct tctacttcat caacaacctc catatctatg ataatcaatt tgaacaagct
    33901 caggaattgc tccgtcggga gccgtcaaac tgccaaccac gcttggtttt aaatgttcct
    33961 gatgggacta atttctttga tatcaaagca gaagattttg agttggtgga ttatgaccct
    34021 gttaagccac agttgaagtt tgacctagct atttaaaaga atagaaaaaa gaagttgaga
    34081 ataatcccaa cttcttttgt ttcttaacgt gatacgcggc gacgagctgc ttttttacgg
    34141 ttttcttcga tgaaagctgc tttttgctct tctggttcga ttactttctt tttaaatgcg
    34201 tatactgcac ctgcaacggc agcgacagtt cctgcgacac ctgttacaag acctttagcg
    34261 aatcctttag ccatgagtct tcctccttta tattctcaat cagccagcct cctcaagagg
    34321 tcacattttt ctgactgacc tttttgtgtt ataataatag taacgaaaaa atgggaattt
    34381 ttcaaggaaa aaagatgaaa acaaaaataa ttgtgattgt tggaccgact gctgttggaa
    34441 agacggctct agctattgaa gttgcaaagc gctttaatgg cgaagtggtt agtggagata
    34501 gccagcaagt ctatcgagga cttgatattg ggacggccaa ggctagtcca gaagagcagg
    34561 cagctgttcc tcatcattta atcgatgtta gagagataac cgagtcttac tcggcttttg
    34621 attttgtttc agaagctaag atgactattg aggatattca gagccgtggc aagctagcca
    34681 ttatcgccgg tgggactgga ctttatatcc agagcttgct agaaggttac cacctaggtg
    34741 gggagactcc tcatgaggag attttagctt atcgagctag tttggagcca tattcagatg
    34801 aggaattagc ccatttggtg gagcaagcag gccttgagat tccccagttt aatcgtcgtc
    34861 gtgctatgcg tgccttagaa attgcccatt ttggtcagga tttggaaaat caagagatct
    34921 tgtatgaacc gctgattatc tgcttggatg atgaacgtag tcaactttat gagcgtatca
    34981 accaccgagt ggatttgatg tttgaggctg ggcttttgga tgaggccaag tggctgtttg
    35041 accattcccc taatgtacag gctgctaaag gaattggcta taaggaactc tttccttatt
    35101 tccgtggaga gcagaccttt gaggaagctc gtgagagtct taaacaggcg acccgtcgtt
    35161 ttgccaagcg tcagctgacc tggttccgta atcgcatgca ggtcaccttt tatcagattg
    35221 gagaatctgg tgtacaagac cgcattttaa gccagataga ggagttttta gatgattgaa
    35281 acggagaaaa aagaggagcg agtcctgctg attggtgtgg aattgcaggg tatggacagt
    35341 tttgacctct ccatggaaga attggctagt ttagcgaaaa cggcaggggc agtcgttgta
    35401 gatagctaca gacaaaaacg tgaaaaatat gattccaaga ccttcgtcgg ctctggtaag
    35461 ttggaagaga ttgcgcttat ggtggatgca gaagaaatca ctactgtcat cgtcaacaat
    35521 cgtctgaccc caaggcagaa tgtcaatcta gaggaagttc tcggtgttaa ggtcattgac
    35581 cgtatgcagt tgattttgga tatctttgcc atgcgggctc gaagccatga agggaagctc
    35641 caagtccacc tagcccaact caaatacctc ttgcctcgct tggttggtca ggggattatg
    35701 ctcagccgtc aggcaggggg aattggttcc cgtggtcctg gtgaaagcca actggagctg
    35761 aaccgtcgta gcgttcgcaa tcaaatcacg gatatcgagc gccagcttaa ggtggttgag
    35821 aaaaatcgtg cgactgtcag agaaaaacgt ttggagtcta gcacttttaa gattggtttg
    35881 attggttata ctaatgctgg gaaatcaact atcatgaaca tcttgaccag taagacccag
    35941 tatgaagcag atgagctctt tgcgactctg gatgcgacaa ccaagagtat tcatctggga
    36001 ggcaatctcc aagtaacttt gacagatacc gttggcttta tccaagattt gccgacagag
    36061 ttggtgtcca gtttcaagtc aaccttggaa gaaagcaagc atgtggacct tctggttcat
    36121 gttatcgatg ctagcaatcc ttaccacgag gagcatgaaa aaacggttct ctccatcatg
    36181 aaagacctgg acatggaaga tattcctcac ttgacgcttt ataataaagc ggatttggtg
    36241 gaggatttca cgcctaccca aacgccatat accctcattt ctgccaagtc tgaggacagt
    36301 cgtgaaaact tgcaagcatt attgctagat aagattaagg aaatttttga agcatttacc
    36361 ctgcgagtgc ctttttcaaa gtcctacaag attcatgatt tagagagtgt tgcaattctg
    36421 gaagaacgtg attatcagga agacggcgaa gtgattacag gctacatttc cgagaaaaat
    36481 aaatggaggt tagaagaatt ttatgactga tattaaaact ttggctctaa agtatggagg
    36541 ttatacaagt ctggacaagg tctatctgga tcagcttcta gctggtaaaa cagatcagga
    36601 gcagttaaca cttattacac ctccgccaag tgttgttaat gcctactttg cagaactcta
    36661 tcagaaaaag agtcctcagg ctgcgacgga ttattttgca gaactcagtc aggaactgaa
    36721 tctttacaat actgagccaa gtttcaccct agaaaataag ccttttattc ggcttaatct
    36781 gtctggtaaa tcctttggtt tttgttatga gagtgacggc cttggtcgga ttttctttga
    36841 aaatgaggag aaaatctcgg atgacttgct ctttgaaatt gcgcaaattt tcccccatca
    36901 attagtcttt gaagagtctg gtaagattta catgaagcct gtcgaggacg aggaagttat
    36961 cagcctagaa agtctcacag ctttgactga tttggaaaga ttggctgatg gtcgaaaacg
    37021 tctcaaaggc tacagccaag aggatttatt gcaagaagct gctgcttttt ctggcaaacg
    37081 ctatttccga tcggaaaacc gcacagccat gttatatatt gattaattag aaagtatcga
    37141 atggatattc aatttttagg aacgggggct ggtcagccct ctaaagcccg caacgtttca
    37201 agtctcgccc tgaaactttt ggatgagatt aacgaagttt ggctctttga ctgtggagaa
    37261 ggtacgcaaa atcgcattct ggaaaccaca attcgaccac gtaaggtcag caaaatcttt
    37321 attacccatc tgcatggaga ccacattttt ggtttgccag gtttcctttc tagccgtgcc
    37381 tttcaggcca atgaagagca gacagatttg gaaatctacg gacctcaagg aatcaagtca
    37441 tttgtcttaa ccagccttcg tgtgtcaggt tctcgtctgc cctaccgcat tcatttccat
    37501 gagtttgacc aagattctct gggtaaaatt cttgaaaccg ataaattcac tgtgtatgca
    37561 gaggagctgg accacactat tttctgtgtt ggctatcgtg tcatgcaaaa ggatctagaa
    37621 gggacgctgg atgctgaaaa actcaaggct gctggtgttc cgttcggccc gctttttggt
    37681 aaaatcaaaa acggccagga tcttgttttg gaagacggaa ctgaaatcaa ggcagcagac
    37741 tatatctcag cgccacgtcc aggtaagatt atcactattt taggagacac tcgaaaaacg
    37801 ggtgccagtg tgcgtctggc tgtcaatgca gatgtcctag ttcatgagtc cacttatggc
    37861 aagggtgatg aaaaaattgc tcgtaaccat ggtcactcaa ctaatatgca agctgcacaa
    37921 gtagcggtag aagcaggtgc caaacgcctc ctactcaacc atatcagtgc ccgtttcctc
    37981 tcaaaagata ttagcaaact caagaaagac gctgccacaa tttttgaaaa tgtccatgtg
    38041 gtcaaagact tggaagaagt ggaaatctag cagtcacaga aaggataagt atgcctacta
    38101 ttctcattac cggagctagc ggtggtctag ctcaagaaat ggtcaaactc ctgcccaatg
    38161 accaactcat cttgcttggt agaaataagg aaaaattagc ccaactctac ggaaattatt
    38221 cccatgcaga attgattgaa attgatatta ccgacgattc agccctagaa gctctggtaa
    38281 ctgatcttta tctccgctat ggcaagattg atgtcttgat taacaacgct ggttacggga
    38341 tttttgaggg atttgaccag attgctgata aagatattca ccagatgttt gaggtcaata
    38401 cctttgccct gatgaatctg tctcgtcacc ttgcggctcg tatgaaggaa agcagcaaag
    38461 ggcatatcat caacatcgtc agcatggcag gtctaatagc tactggcaag tctagtcttt
    38521 actcagcgac caagtttgcg gctattggtt tttcaaatgc tctgcgactc gaacttatgc
    38581 cctatggagt ctatgtgaca acagtcaatc caggtccaat ccgaacagga tttttcgacc
    38641 aagccgaccc agatggaact tatcttaaat cggttgaccg cttcctgcta gaaccagatg
    38701 cagtggctaa aaagattgtc aagattatag gaaaaaacaa acgagaactc aatctcccga
    38761 ttttgttgaa cctagctcat aagttttata ccctctttcc taagctagct gataagttgg
    38821 caggggaaac ttttaattat aagtaaaaag aaccaatgtg caggttgttg ctagcctaca
    38881 tattggttct tatcatttct caactatcaa actgaacttc ttctaggaga tagtctataa
    38941 agcgttcgcc catcttagat aggctggttt tctcatgctg gatatagacc agctcgatcg
    39001 ggtcatcaat atccagtggg atagaaacga tattgtctcc gtttaggttg ctgttcaaaa
    39061 tccctgtcgc aatggtataa ccatccaaac caatcaagag attaaagagg gtggcacggt
    39121 cactgaccac aatggatttc ttgtggtgtt cttgagaaag aatctcttct gaaaagtaga
    39181 aggagttgtg cgtcccttgg tcatagctga ggtaagggaa attctccaaa tcagacagtt
    39241 tcaccttgtc tttctttgcc agagggttgg tcttgctgac aaagatatgt ggttgcgctg
    39301 tgaagagatg gtgggctagc aggtgattgt catccagcat cttggttaaa acatcacggt
    39361 tgtaactgtt taagaagagg accccgacct cactgcggaa gttcttgacg tcgtcgataa
    39421 tctcccaagt ccgagtttca cgaaggaaga gttcgtattt ctccatatcg cttttcttga
    39481 gcaaagagac aaaggcattg accacaaagg catagtgttg agacgaaacg ctaaatagtt
    39541 cgcggtgggc gacaggattt ttatagcgtt cctccagaag ctgggtctgc tcgacaacct
    39601 gacgggcata agagagaaac tccatcccat cacgggtcaa ggtgattccc ttgggattgc
    39661 ggataaagat ctcaatgccc atttcatttt ccaaatctcg cactgcattg gagagacttg
    39721 gctgagtgat aaagagttgc ttggctgcct cattcatgga gccagtttcg acgattttga
    39781 taatataatg taattgttga attctcatgt ttttattgta ccatacttgc cttagaatcg
    39841 ctaaagaaga taggaaaata aggagcattg tgtgagtctc ttcttgaaaa aaacacaaaa
    39901 ttttgctaga atgagtctta tgaaaacatt ctatgatgtg cagcaattcc tcaaacgatt
    39961 tggtattatt gtttacatgg gaaaacgctt atatgatatt gaactgatga agttggaact
    40021 atctcggatt tacgatgcag ggttgatgga taaattagac tatctagagg cagaagcagt
    40081 tcttcgcaga gagcacaagg tagaattgga ttatattgag aaaaatggag aaaagaacta
    40141 atggttactt ggattttgtg ggcacttata ctagcaatgt tggcgtggat gggctttaac
    40201 tatcttcgta ttcgccgtgc ggctaaaatt gtggacaatg aggagtttga agccttgatt
    40261 cgtacgggtc aattgattga tttgcgcgac ccagcagaat tccacagaaa acatatcctt
    40321 ggtgcacgca atattccttc aagtcagttg aaaactagtc ttgcagccct tcgtaaagat
    40381 aaacctgtcc ttctctacga gaaccaacgt gcgcaacgag ttacaaatgc agctctttac
    40441 ttgaaaaaac aaggtttttc tgagatttat atcctttctt atggcttgga ttcttggaaa
    40501 gggaaagtga agactagcta accttatttc caaattagtc gaatgatagg atgaagctat
    40561 tgttttatag tataattaat ttgtataaaa tttaaggagt tcttagaaca taaaagaaaa
    40621 gaagaaaaat ccactttttg tgggtatctt gtcgattatt cttggtttgt tatttccaat
    40681 tgtaggcctg attttgggta tcataggatt ggtcttggct atttcatacc aaaaagagtc
    40741 ataattagac tataaaatag aaaagattct taatatctta ggaattgtga tttctgtagt
    40801 taactggatt gtagcaatcg ccttaatttt tcggtaaata aaagaaaaaa gctttagttt
    40861 taaagctttt tttgttttat cgactcatct ctaaatagtt tttaatgatt tgtaactctt
    40921 tttggttcaa agggcggtag ttactttctg ctaaatctgg gttcaatgtg aagtccccaa
    40981 attggattct cttgaggata gtcaccttaa caccaaccga gaggaacatt ttcttgattt
    41041 gatgaaattt tccttctgaa atggtgatag aggcttgact gatggaggga cttgcagata
    41101 gaatctctag ttttgcgggt ttacagacag taccatctaa aaagacaatt cccttttgaa
    41161 aggcttggat atggtcaggt gttagaagtc cattaacctc aacttgataa gtcttatcga
    41221 catgatattg gggatggagg agttggaagc ccaagggacc gttatcggtc aagaggagga
    41281 gtcccgttgt atctcggtcc agtcggccaa cggcatagag cttgtcagac tggatgtcag
    41341 atggaagcag gtccatgacg gtcggaagtt ccttgtcttt gttggctgta acgacaccag
    41401 caggtttatg aagcataaga taggtgtgtt catagccttg aatgattcga tcctgaaaaa
    41461 ggagttcttg tagtcctgta tcgatatttt gagctaggga gcgggctggg caaccatcga
    41521 ctagaatttt ccctctgagg agtgcttgct tcatggcctt tcggctaact ttttcttggg
    41581 ctaataaatt atctaaacgc atactgtgcc tatcttatca taagtcgctt gctttgaaaa
    41641 ggcttgacgt gaaaagaaaa gcggagtgaa aacatttaca cttgcttgag ttatgttatt
    41701 tatttgaaat tatggtataa tcgttcagtt agaaaataaa ttttgaatat tatagaggaa
    41761 atcatgacaa aattaagaga agatatccgt aacattgcga ttatcgccca cgttgaccac
    41821 ggtaaaacaa ccctggttga cgaattattg aaacaatcag aaacgcttga tgcacgtact
    41881 gaattggcag agcgtgctat ggactcaaac gatatcgaaa aagagcgtgg aatcaccatc
    41941 cttgctaaaa atactgccgt tgcttacaac ggaactcgta tcaacattat ggacacacca
    42001 ggacacgcgg acttcggtgg agaagttgag cgtatcatga aaatggttga cggtgttgtc
    42061 ttggtcgtag atgcctatga aggaaccatg ccacaaactc gtttcgtatt gaaaaaagcc
    42121 ttggaacaag accttgtccc aatcgtggtt gttaacaaaa tcgataagcc atcagctcgt
    42181 ccagcagaag tagtggatga agtcttggaa cttttcatcg agcttggtgc agatgacgac
    42241 cagcttgatt tcccagtggt ttatgcttca gcgatcaacg gaacttcttc attgtcagat
    42301 gatccagctg accaagaagc gactatggca ccaatctttg acacgattat cgaccatatc
    42361 ccagctccag tagataactc agatgagcct ttgcagttcc aagtgtcact tttggactac
    42421 aatgacttcg ttggacgtat cggtatcggt cgtgtcttcc gtggtacagt taaggttggg
    42481 gaccaagtta ccctttctaa acttgacggt acaactaaaa acttccgtgt tacaaaactc
    42541 ttcggtttct ttggtttgga acgtcgtgaa atccaagaag ccaaagcggg tgacttgatt
    42601 gccgtttcag gtatggaaga catctttgtc ggtgaaacca tcactccgac agatgcagta
    42661 gaagctcttc caatcctaca catcgatgag ccaactcttc aaatgacttt cttggtcaac
    42721 aactcaccat ttgctggtaa agaaggtaaa tgggtaactt ctcgtaaggt ggaagaacgc
    42781 ttgcaggcag aattgcaaac agacgtttcc cttcgtgttg acccaactga ttcaccagat
    42841 aaatggactg tttcaggacg tggagaattg cacttgtcaa tccttatcga aacaatgcgt
    42901 cgtgagggct atgaacttca agtatctcgt ccagaagtta tcgtaaaaga aatcgacggt
    42961 gttaaatgtg aaccatttga acgtgtacaa atcgacactc cagaagaata ccaagggtct
    43021 gttatccaaa gcctttctga acgtaaaggt gaaatgttgg atatgatttc aactggtaat
    43081 ggtcaaactc gtttggtctt ccttgttcca gcgcgtggtt tgattggata ctcaactgag
    43141 ttcttgtcaa tgactcgtgg ttacggtatc atgaaccata ccttcgacca atacttgcca
    43201 ttgattccag gggaaattgg tggacgtcac cgtggtgccc ttgtttctat cgatgctggt
    43261 aaggctacaa cttactcaat catgtctatc gaagaacgtg gtacgatctt tgtcaaccca
    43321 ggtactgagg tttatgaagg aatgatcatc ggtgaaaact ctcgtgaaaa tgacttgaca
    43381 gttaacatca cgaaggcaaa acaaatgacc aacgttcgtt cagctactaa ggaccaaaca
    43441 gctgttatca agacgcctcg tatcttgaca cttgaagagt ctcttgagtt cttgaacgac
    43501 gatgagtaca tggaagtaac gcctgagtct atccgtttgc gtaaacaaat ccttaacaaa
    43561 gcagagcgtg agaaagctaa caagaagaaa aaatcagctg aataagagct agaaagagag
    43621 aaagatggtc tatttagtcc taggaatttt actgctccta ctctatgtat ttgcgacacc
    43681 agaaagcatt aaagggactg tcaatatcgt cgctatggta tgtattttag tggcactctt
    43741 gattttattg gttctatctt ttctgaaaat ttttcaatta ccaacagaaa tattcctagc
    43801 aatagccatg ttgatcctag cttactttag tgtgagagac atcacactca tgccagtcaa
    43861 aaaaagtaaa agaagataaa aaagagagtt tcggctctct ttttgctagg aattttacaa
    43921 tagtttgaat tgctttttag ttaacaccat ctcttttact tgtatagtat gaatgggaaa
    43981 aacttgaata aaattcgtta atggcatcta ttttatattt ggttagtact ggttgcatat
    44041 tattttgaaa taaaactact atataatatt attcatagag ataatatagt aaaacatcta
    44101 aaaaattcga ttttttcctt gacaacaaat aataaaaatg ttattcttat cacataagaa
    44161 agcgatttca acaaaagaga gaggaggatt tattttgttg cattgttgct caaggtgtga
    44221 tttttttgaa agaaaaaata aaatgaaaac gaaaaaacat agattacttg ctctagctct
    44281 tatttcaaat tttacattat tgggagctgc atcagctgct gtacaatatc cagatggagg
    44341 agtatggaca tatggagaag gttcaggagg tggttaggct ttttcaaatt actatcatgg
    44401 taaaaaatat cattattcat ccatcgtgag cagatgggat ggtcattctg ataaaggaga
    44461 agctcctgct ggaaaaacct cctatgcatg gatttggact aaatggggag aacaagtagc
    44521 attttactat gactatgact aatatttagt tttcttagat aattcccctt gtaagaaatt
    44581 acagggggaa ttttgtttac aaggagtcta ctatgaagaa actattcata ttattatcaa
    44641 ctttttttct cagcttcttc cttgcttgga ttattgtctt acgtgcgcca caatatttat
    44701 atgcaagcta tgattccgtt tctttacttc gtgtcaaaaa agatactcag gaaccgacgc
    44761 gtgaggtatt tgaacaggaa ttggagaatt ttgcaaactc agaacagagt ttaatagcta
    44821 gaagaattgt agagccgagt aaggatggga cgactcactt tacttatgca acttatggtc
    44881 agggaacttt accaaaagaa ttccaagaag ctagccaaga aagtcgtgaa cgtagtgatc
    44941 cgctaaatag ttatctcctt ttgtcaggct ccttgacgaa agaaaagctt gccgataaat
    45001 taggagattt gggttataaa gcaagtgctg accgaaagat accgccctat tttcttgctt
    45061 ttcgaatatt actaaatccc cttattttaa ttagtttagc aatatttggc ttatctttct
    45121 ttgctttagt gattatcact cggattaagg aaatgagagc agcaggtata aaactctttt
    45181 ctggtcagac tctcttatcc atcatggggc attctttatc gactgatatc aaatggctcc
    45241 ttctatcagc cctcctttcc ttcctaggtg ggggagtcgt tctttttagt caaggtttgt
    45301 tttatcctat cttgttagcc acctatggtt ttgggattag tttctatctg ttgtttttat
    45361 tggcgatttc aattttacta atgcttcttt atctaatgag tttgaattac aaagcattag
    45421 ttcccgttat taaggggaga ttacccctta aacgcctgat gattttaacc ctattgtgtc
    45481 agttagtagc tgtttttaca gtaggctacg ctgttaagac aggtttgacg tcttaccaac
    45541 gattgaaaga acttgaaatt tcaaaacaag catggcagga tagagcagac tattatcaaa
    45601 tttcttttgg cttaggtgat agaggaaaag atacagaaaa tcagagcaag tggtatgcct
    45661 ttgccaagga agcaatcgaa gaagaacaag ctctttatgt aaaggataat ctgctccatt
    45721 ttgccaatcc acaaggaaaa aatgaacagg gagagacact ggatacctat agtccagatg
    45781 ctaatacgct ctatgttagt cccagttatt tggacaagga aaaggtcgtg gtagatgctg
    45841 agaccaaaca gaagctagcc catctccaaa aaggtgagtt tatcctcttg ctcccagaac
    45901 atttgcgctc tcgagaagca gaacttaaga aagtttttga agaaagattg agttattatg
    45961 gaaaatctgg tgaggaggca agtgctcctt tggattatga gatgaaagcg cacgttagtt
    46021 atctttcaat gggagaaaag cggtttgttt ataataacgg tgagaatccc gtatccactc
    46081 agtatttgac tgatccgatt ttagtggtat tcacgccgac ttctacaggt gatagtttta
    46141 tatccttatc tagttggtct atcaatgctg gaaaacaact ctttatcaaa ggatatgaga
    46201 gtgggctaga actcttgaag aaagctggaa tttatgagca agtatcctat cttaaagaag
    46261 gaagaagtgt ttatctaact cgttataatg aagttcaaac tgaaacagca actttaatct
    46321 taggagctat tgtggggata gctagttcct tgttactatt ttattctgtc aatcttctat
    46381 atttcgagca attccgccga gatattttga ttaaacgaat ttcaggttta cgattttttg
    46441 aaacacatgc tcagtatatg gttagccaat ttgccagttt tgtatttggt gctagtttct
    46501 ttattttacg cagtagagac tgggtaattg gcttactcac tttattagtc tttctagcta
    46561 gtgcagtttt gacgctttac cgtcaagcac agaaagaatc tcgtgtttct atgacaatta
    46621 tgaaaggaaa ataggatgat tgaactaaag aatatatcta aaaaatttgg aagccgtcag
    46681 ctattttcag atacgaatct tcattttgaa ggtgggaaaa tttatgcctt aatcggtaca
    46741 agtggctgtg gtaagacaac actcttgaat atgattggac gattagagcc atatgacaaa
    46801 gggcaaatca tctatgatgg tacttctctt aaggacatca agccttctgt tttctttaga
    46861 gattacttag gatatttatt tcaagatttt ggcttaattg aaagccaaac tgtcaaagag
    46921 aatctcgatc tgggtttagt tggtaaaaag ttgaaggaaa aagagaaaat ctctttgatg
    46981 aaacaagctc taaatcgtgt aaacctctct tatttggatt tgaagcaacc tatatttgag
    47041 ttatcaggag gagaagcaca acgtgttgca ctagcgaaga taattttaaa ggatccacct
    47101 ttgatcctcg cagatgaacc aaccgcttcc ttagacccta aaaactctga ggaattactt
    47161 tccatcctag aatctttaaa aaatccgaat cggaccatta ttattgcgac ccacaatcct
    47221 ctgatttggg ggcaagtgga tcaggtcatt cgagttaccg atttatcaca tagatgatat
    47281 ggtaagattc agttagaaga aagagtcaca aacacacttt gtggcttttt tatttccata
    47341 aaaatggtaa aatagtagga gtagaaatgg agttcgagac atgaaagtaa tagatcaatt
    47401 taaaaataag aaagttcttg ttttaggttt ggccaagtct ggtgaatctg cggctcgttt
    47461 gttggacaag ttaggtgcca ttgtgacagt aaatgatggg aaaccattcg aggacaatcc
    47521 agctgcccaa tgtttgctgg aagaagggat caaggtcatt acaggtggcc atcctttgga
    47581 actcttggat gaagagtttg ccctcatggt aaaaaatcca ggtatcccct acagcaatcc
    47641 catgattgaa aaggctttgg ccaagggaat tccagtcttg actgaggtgg aattggctta
    47701 tttgatttca gaagcaccga ttattggtat cacaggatcg aacggtaaga caaccacaac
    47761 gactatgatt ggggaagttt tgactgctgc tgggcaacat ggtcttttat cagggaatat
    47821 cggctatcct gccagtcagg ttgctcaaat agcatcagat aaggacacgc ttgttatgga
    47881 actttcttct ttccaactca tgggtgttca agaattccat ccagagattg cggttattac
    47941 caacctcatg ccaactcata tcgactacca tgggtcattt tcggaatatg tagcagccaa
    48001 gtggaatatc cagaacaaga tgacagcagc tgatttcctt gtcttgaact ttaatcaaga
    48061 cttggcaaaa gacttgactt ccaagacaga agccactgtt gtaccatttt caacacttga
    48121 aaaggttgat ggagcttatc tggaagatgg tcaactctac ttccgtggtg aagtagtcat
    48181 ggcagcgaat gaaatcggtg ttccaggtag ccacaatgtg gaaaatgccc ttgcgactat
    48241 tgctgtagcc aagcttcgtg gtgtggacaa tcaaaccatc aaggaaactc tttcagcctt
    48301 cggtggtgtc aaacaccgtc tccagtttgt ggatgacatc aagggtgtta aattctataa
    48361 cgacagtaaa tcaactaata tcttggctac tcaaaaagcc ttgtcaggat ttgacaacag
    48421 caaggtcgtc ttgattgcag gtggtttgga ccgtggcaat gagtttgacg aattggtgcc
    48481 agacattact ggactcaaga agatggtcat cctgggtcaa tctgcagaac gtgtcaaacg
    48541 ggcagcagac aaggctggtg tcgcttatgt ggaggcgaca gatattgcag atgcgacccg
    48601 caaggcctat gaacttgcga ctcaaggaga tgtggttctt cttagtcctg ccaatgccag
    48661 ctgggatatg tatgctaact ttgaagtacg tggcgacctc tttatcgaca cagtagcgga
    48721 gttaaaagaa taatatgaaa aaaattgtct ttacaggtgg ggggacggtt ggacacgtta
    48781 ccctcaatct tttgttaatg cctaagttca tcgaagatgg ttgggaagtc cactatatcg
    48841 gggacaagcg tggtatcgaa caccaagaaa tccttaagtc aggtttggat gtcaccttcc
    48901 attctattgc gactggaaaa ttgcgtcgct atttctcttg gcaaaatatg ctggatgtct
    48961 tcaaagttgg ttggggaatt gtccaatcgc tctttatcat gttgcgactg cgtccacagg
    49021 ctcttttttc aaagggagga tttgtctcag taccgcctgt tatcgctgcg tgtgtgtcag
    49081 gagtgcctgt ctttattcac gaatctgacc tgtctatggg cttggccaat aaaatcgcct
    49141 ataaatttgc gactaagatg tattcaacct ttgagcaagc ttcgagtttg tctaaggttg
    49201 agcatgtggg agcagtgacc aaggtttcgg atcaaaaaaa tccagaacca gatgaattgg
    49261 tggatattca aagccacttt aatcataaat tgccgactgt attgtttgtt ggcggttctg
    49321 caggtgctcg tgtctttaac caattggtga cagaccataa gaaagaacta acagagcgct
    49381 acaatattat caatctaact ggagattcta gcctgaatga gttgagccaa aatctttttc
    49441 gtgttgacta tgtgaccgat ctctatcaac ccttgatgga attggctgat attgttgtga
    49501 cacgtggtgg tgccaatacg atttttgagc tcttggcgat agcaaaattg catgtcattg
    49561 tgccacttgg tcgtgaagct agtcgtggtg accagattga aaatgcagct tactttgtta
    49621 agaaaggcta tgcagaagac cttcaagaaa gcgatttgac cttggatagt ttggaagaga
    49681 agctttctca cttactaagt cacaaggaag attaccaagc taagatgaaa gcttctaagg
    49741 aattgaaatc tctagcagat ttttatcaat tgttgaaaaa agatttatca taaggaaagt
    49801 aaatgtcaaa agataagaaa aatgaggaca aagaaatcct cgaagaattg aaagagttat
    49861 cagaatggca gaaacgaaac caagaatatc taaaaaagaa ggctgaagaa gaagcggctc
    49921 tagctgagga gaaggaaaag gaaagacaag ctcgaatggg agaagaatct gagaagtcag
    49981 aggacaaaca ggaccaggag agtgaaacag accaggaaga ttcagaatca gctaaggaag
    50041 agtctgaaga aaaagtagca tcctcagagg ctgacaaaga gaaagaagag aaagaagaac
    50101 cagagtctaa agagaaggag gaacaggata aaaagcttgc taaaaaggct acaaaggaaa
    50161 aaccagccaa agcaaagatt cctggtatcc atatcttgcg agccttcacg attttatttc
    50221 caagtctgct tttattgatt gtctctgcct acttgctcag tccttatgcg accatgaaag
    50281 atattcgtgt tgagggaacg gtgcaaacta cagctgatga tattcgacag gcttcaggca
    50341 ttcaggattc ggattatacg attaaccttc tgctagacaa ggcaaaatat gaaaagcaga
    50401 ttaagtctaa ctattgggtt gaatcagctc aacttgtcta tcaatttcca actaagttca
    50461 ctattaaggt caaggaatat gatattgtgg cctactatat ttctggtgaa aatcattatc
    50521 ctattctttc cagtggtcag cttgagacta gttctgtgag tctgaacagt ttaccagaaa
    50581 cttatatatc agttctcttt aatgacagcg aacaaatcaa agcctttacg tcagaacttg
    50641 ctcaaattaa cccagaactc aaggtggcta tccaaaaggt ggaattagcc ccaagcaagg
    50701 tgacttccga tttaattcga ttgactatga atgattcgga cgaagtcttg gttccgcttt
    50761 ctgaaatgag taagaaattg ccttattaca gtaagattaa gcctcaattg tcagaaccaa
    50821 gtgtggtcga tatggaagct ggagtctaca gctacactgt agcggataaa ttgattatgg
    50881 aagcagaaga aaaagctaaa aaagaggccg aagaagctga gaaaaggcag gaagaggaac
    50941 gtaaacgttt agaagaagaa cagaaaaaac aagaggaaga gagcaatcga aaccaaacga
    51001 cccagcgctc atcgcgtcgc taggttttac cttttctcct atagctcttt agttgccatg
    51061 tttttacgtt taatatttga catctgtttt tctttatgtt atatttgcaa tgtaatcgat
    51121 tacaaaaaaa ttttttatga agaaggtaac acatatgtca gatgaagtat tcttatttga
    51181 agaaattgaa gaaatcgtag cccctacaga tggtgaattt tgggggggag ttttacttgg
    51241 aactggggta gttctcttaa ttggagtagc ctgttgttaa aaagataggg agtgataatc
    51301 atgcaagata actttttatt tgaggaaatt gaagaaattt cagtaccagt tagtgatttt
    51361 tcagctggac ttgcaacagg tatcggatta ggtttagcaa tccttgctct tgctggttgt
    51421 tgaagtttgt tcacttacta atatcaagct ttttcaattt cattttagac agtcatttga
    51481 attttccgta ttagtcttgc agcaagagat taatagaatt agtcattatt ttattgattg
    51541 cggactgagg gactagagta tgttttactt agcccctctt ttatttatta aaggttaggt
    51601 ttgttatgag aattgttgat aagattaaga tactacctac tccttatgag ggacagtatc
    51661 atctatatat accatccagt aagaaacatg tattagttgg gaaacaggaa aaaaaatggt
    51721 tagagcaact aataggtcaa gaatttacca tatcggactt attagtgtta gtagggaaga
    51781 aatattattt aagattttat attgttctta gtaaaatggg acttatagat tctatgaaat
    51841 cacgaaaaaa gataaatatt tttaaaatat cttgggactt taatata
//
LOCUS       DAVKLW010000008        45530 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKLW010000008 DAVKLW010000000
VERSION     DAVKLW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.008"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            548..1000
                     /locus_tag="VW324_000450"
     CDS             548..1000
                     /locus_tag="VW324_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9726129.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGEVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1019..2356
                     /locus_tag="VW324_000451"
     CDS             1019..2356
                     /locus_tag="VW324_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9726130.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2435..3319
                     /locus_tag="VW324_000452"
     CDS             2435..3319
                     /locus_tag="VW324_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9726131.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3319..4152
                     /locus_tag="VW324_000453"
     CDS             3319..4152
                     /locus_tag="VW324_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9726132.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4171..6264
                     /gene="nanB"
                     /locus_tag="VW324_000454"
     CDS             4171..6264
                     /gene="nanB"
                     /locus_tag="VW324_000454"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9726133.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFYETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6277..7380
                     /locus_tag="VW324_000455"
     CDS             6277..7380
                     /locus_tag="VW324_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9726134.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7554..8252
                     /locus_tag="VW324_000456"
     CDS             7554..8252
                     /locus_tag="VW324_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9726135.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8271..9788
                     /locus_tag="VW324_000457"
     CDS             8271..9788
                     /locus_tag="VW324_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9726136.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9913..11241
                     /locus_tag="VW324_000458"
     CDS             9913..11241
                     /locus_tag="VW324_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9726137.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11493..12359
                     /locus_tag="VW324_000459"
     CDS             11493..12359
                     /locus_tag="VW324_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9726138.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12375..13214
                     /locus_tag="VW324_000460"
     CDS             12375..13214
                     /locus_tag="VW324_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9726139.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13486..13938
                     /locus_tag="VW324_000461"
     CDS             13486..13938
                     /locus_tag="VW324_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9726140.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14080..14388
                     /locus_tag="VW324_000462"
     CDS             14080..14388
                     /locus_tag="VW324_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726141.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14398..14613
                     /locus_tag="VW324_000463"
     CDS             14398..14613
                     /locus_tag="VW324_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726142.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKSPKEKRKNRFVHGRDL"
     gene            14671..15315
                     /locus_tag="VW324_000464"
     CDS             14671..15315
                     /locus_tag="VW324_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9726143.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLAFLQVGVMEKLFAKYE"
     gene            15488..16405
                     /locus_tag="VW324_000465"
     CDS             15488..16405
                     /locus_tag="VW324_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9726144.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEILKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVIEEDRPVVEAAAALIRETKERFL"
     gene            16423..17307
                     /locus_tag="VW324_000466"
     CDS             16423..17307
                     /locus_tag="VW324_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9726145.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17323..18174)
                     /locus_tag="VW324_000467"
     CDS             complement(17323..18174)
                     /locus_tag="VW324_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9726146.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18374..20431
                     /gene="pbp2b"
                     /locus_tag="VW324_000468"
     CDS             18374..20431
                     /gene="pbp2b"
                     /locus_tag="VW324_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9726147.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKELP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20442..21038
                     /gene="recR"
                     /locus_tag="VW324_000469"
     CDS             20442..21038
                     /gene="recR"
                     /locus_tag="VW324_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9726148.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21214..22257
                     /locus_tag="VW324_000470"
     CDS             21214..22257
                     /locus_tag="VW324_000470"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9726149.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPGLIERLVDL
                     AKESFDKREAHLI"
     gene            22341..23714
                     /locus_tag="VW324_000471"
     CDS             22341..23714
                     /locus_tag="VW324_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9726150.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGQDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23701..24312
                     /locus_tag="VW324_000472"
     CDS             23701..24312
                     /locus_tag="VW324_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9726151.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24403..25107
                     /locus_tag="VW324_000473"
     CDS             24403..25107
                     /locus_tag="VW324_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9726152.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25326..26699
                     /gene="ftsA"
                     /locus_tag="VW324_000474"
     CDS             25326..26699
                     /gene="ftsA"
                     /locus_tag="VW324_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9726153.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26716..27975
                     /gene="ftsZ"
                     /locus_tag="VW324_000475"
     CDS             26716..27975
                     /gene="ftsZ"
                     /locus_tag="VW324_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9726154.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27980..28651
                     /locus_tag="VW324_000476"
     CDS             27980..28651
                     /locus_tag="VW324_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9726155.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28661..29200
                     /locus_tag="VW324_000477"
     CDS             28661..29200
                     /locus_tag="VW324_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9726156.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29200..29463
                     /locus_tag="VW324_000478"
     CDS             29200..29463
                     /locus_tag="VW324_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9726157.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29460..30245
                     /locus_tag="VW324_000479"
     CDS             29460..30245
                     /locus_tag="VW324_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9726158.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEYL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30254..31042
                     /gene="divIVA"
                     /locus_tag="VW324_000480"
     CDS             30254..31042
                     /gene="divIVA"
                     /locus_tag="VW324_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9726159.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31390..34182
                     /locus_tag="VW324_000481"
     CDS             31390..34182
                     /locus_tag="VW324_000481"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9726160.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPVGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     ICFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            34592..34954
                     /locus_tag="VW324_000482"
     CDS             34592..34954
                     /locus_tag="VW324_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726161.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGRILA
                     IIGGSLFLATLKKFKSEE"
     gene            35489..36181
                     /locus_tag="VW324_000483"
     CDS             35489..36181
                     /locus_tag="VW324_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9726162.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36234..38621
                     /locus_tag="VW324_000484"
     CDS             36234..38621
                     /locus_tag="VW324_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9726163.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GSERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNSDYHLNVNLQMNY
                     WPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHTQATPFGWT
                     APGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFWNAFLHKDQ
                     QAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLLTEVKEKSD
                     LLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSYKGQEYIEA
                     ARASLNDRGDGGTGWSEANKINLWARLGDGNRAHKLLAEQLKTSTLQNLWCSHPPFQI
                     DGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSMSWEDKKLL
                     QLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDLVQFYF"
     gene            38871..39572
                     /locus_tag="VW324_000485"
     CDS             38871..39572
                     /locus_tag="VW324_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726164.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39574..42282
                     /locus_tag="VW324_000486"
     CDS             39574..42282
                     /locus_tag="VW324_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9726165.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42378..42869)
                     /gene="tpx"
                     /locus_tag="VW324_000487"
     CDS             complement(42378..42869)
                     /gene="tpx"
                     /locus_tag="VW324_000487"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9726166.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(42992..43921)
                     /gene="psaA"
                     /locus_tag="VW324_000488"
     CDS             complement(42992..43921)
                     /gene="psaA"
                     /locus_tag="VW324_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9726167.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(43953..44801)
                     /locus_tag="VW324_000489"
     CDS             complement(43953..44801)
                     /locus_tag="VW324_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9726168.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44798..45520)
                     /locus_tag="VW324_000490"
     CDS             complement(44798..45520)
                     /locus_tag="VW324_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9726169.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        13830 a         8131 c        10025 g        13544 t
ORIGIN      
        1 ttagagaatg aaatatagag tatttctctc ttaaaagttt ttggtgaaac gagatgtaga
       61 aaggagattt agccaaagag tctattagtg ctagaataat agattagaat tattttagaa
      121 aaacgaagtg agcagcttat aaattcaagt ccccaaatag attcatacta gtatcttttg
      181 caaaaaataa agggcgattt ccttcatgaa tatcaatttc atctataagg aaggtagcta
      241 attgaactaa cttatttatt ctgtttgtcg ctagaaaaat cagacctcct tgtgaagatt
      301 gaggagatac tgaatgaaaa tcaaagaaga aactagcaag ctagtagcag attgcccaaa
      361 acaccgcttt gaggttgtag ataagactga cgtatataat ccaaggtgaa gcgactgtgg
      421 tttgaagaga ttttcaaaga gtataggcta gagagtagtg tttttatgtc cttctagtag
      481 aaaatgctag acagaagaat ggggaacttg gataggaaaa atagattgag aaaggaggtt
      541 agaagagatg attattacaa aaattagccg tttaggaact tatgtgggag taaatccaca
      601 ttttgcaaca ttaatagatt ttttagaaaa aacaggacta gaaaatttaa cagaaggttc
      661 gattgctatc gatggtaatc gattgtttgg gaattgcttt acttatctag cagatggtca
      721 agcaggagcc ttctttgaaa cccaccaaaa atatttggat attcatttag tgctggaaaa
      781 cgaagaagcc atggctgtga cgtcgccgga aaatgtaagc gttacccaag agtatgatga
      841 agagaaggat attgaattat acacagggga agtggaacag ttagttcatt tgagagctgg
      901 cgaatgcctc atcacttttc cagaagattt acatcaaccc aaggttcgta taaatgatga
      961 acctgtgaaa aaagttgtct ttaaagttgc gatttcttaa tgtagaaaga gaagaacgat
     1021 gaaaaaaatg agaaagtttt tatgtctagc tggaattgcg ctagcggctg ttgccttggt
     1081 agcttgttca ggaaaaaaag aagctacaac tagtactgaa ccaccaacag aattatctgg
     1141 tgagattaca atgtggcact cctttactca aggaccccgt ttagaaagta ttcaaaaatc
     1201 agcagatgct ttcatgcaaa agcatccaaa aacgaaaatc aagattgaaa cattttcttg
     1261 gaatgacttc tatactaaat ggactacagg tttagcaaat ggaaatgtgc cagatatcag
     1321 tacagctctt cctaaccaag taatggaaat ggtcaactca gatgctttgg ttccgctaaa
     1381 tgattctatc aagcgtattg gacaagataa atttaacgaa actgccttaa atgaagcaaa
     1441 aatcggagat gattactact ctgttcctct ttattcacat gcacaagtca tgtgggttag
     1501 aacagatttg ttaaaagaac ataatattga ggttcctaaa acttgggatc aactctatga
     1561 agcttctaaa aaattgaaag aagctggagt ttatggcttg tctgttccgt ttggaacaaa
     1621 tgacttaatg gcaacacgtt tcttgaactt ctacgtacgt agtggtggag gaagcctctt
     1681 aacaaaagat cttaaagcag acttgacaag ccaacttgct caagatggta ttaaatactg
     1741 ggttaaattg tataaagaaa tctcacctca agattctttg aactttaatg tccttcaaca
     1801 agctaccttg ttctatcaag gaaaaacagc atttgacttt aactctggct tccatatcgg
     1861 aggaattaat gccaacagtc ctcaattgat tgattcgatt gatgcttatc ctattccaaa
     1921 aatcaaagag tctgataaag accaaggaat tgaaacctca aacattccaa tggttgtttg
     1981 gaaaaattca aaacatccag aagttgctaa agcattctta gaagcacttt ataatgaaga
     2041 agactacgtt aaattccttg attcaactcc agtaggtatg ttgccaacta ttaaggggat
     2101 tagcgattct gcagcctata aagaaaatga aactcgtaag aagtttaaac atgctgaaga
     2161 agtaattact gaagctgtta aaaaaggtac tgctattggt tatgaaaatg gaccaagtgt
     2221 acaagctggt atgttgacta accaacacat tattgaacaa atgttccaag atatcattac
     2281 aaatggaaca gatcctatga aagcagcaaa agaagcagaa aaacaattaa atgatttatt
     2341 tgaggctgtt cagtagatgt aaaagactag aaaataggtg ggctagtgag ctgaaaagct
     2401 ctagcccaat cttgtaaaag aagggagaag gagaatggtt aaagaacgta atttaactcg
     2461 ctggatattt gttttgccag ctatgattat cgtaggatta ctctttgttt atccgttttt
     2521 ctcgagtatt ttttatagct ttaccaataa gcatttgatt atgcctaatt ataaatttgt
     2581 tggtttggct aactataaag ctgtgctatc agatcccaac ttctttaatg cgttctttaa
     2641 ttcaattaag tggaccgttt tctcattagt tggtcaagtt ttagtagggt ttgtattggc
     2701 tttagctctt cacagagtac gccacttcaa gaaattatat aggaccttat tgattgttcc
     2761 ttgggcattt cctaccatcg ttattgcctt ttcttggcag tggattctaa acggggttta
     2821 tggctactta cctaatctaa tcgtaaaatt aggtttaatg gaacatacac ctgcattttt
     2881 gacagatagt acatgggcat tcctatgttt ggtgtttatc aacatttggt ttggagcacc
     2941 aatgattatg gttaatgtgc tttcagcttt gcaaacagta ccagaagaac aatttgaggc
     3001 tgctaagata gatggtgctt caagttggca ggtgttcaag tttatcgtct ttccacatat
     3061 taaagtggtt gtaggacttc tagttgtttt gagaactgta tggatcttta ataactttga
     3121 cattatctac ctcattactg gtggtggacc agccaatgct acaacgacgc ttccaatttt
     3181 tgcttacaac ctgggttggg gaactaaatt gttgggtcgt gcttcagcag ttacagtatt
     3241 gctctttatc ttcttggtgg cgatttgctt tatctacttt gctatcatca gtaagtggga
     3301 aaaggagggt agaaaataat gaagaagaaa tccagtattt atttagatat tctctcacat
     3361 gtacttttag ttggtgcgac catcgttgca attttcccat tggtatggat tatcatatct
     3421 tctgtcaaag ggaaagggga attaactcag tatccaacac gattttggcc tgaacagttt
     3481 acattagatt atttcactca tgttatcaac gatttgcact tcattgataa cattcgaaac
     3541 agtttaatca ttgccttggc tacaaccctc attgcgatta ttatttctgc tatggcagcc
     3601 tatggtattg ttcgattctt tcctaaattg ggagcaatca tgtcgagact actcgtcatt
     3661 acctatattt tcccaccaat tttgttagca attccctatt caattgccat tgctaaagtt
     3721 gggttaacaa atagtttatt tggcttgatg atggtttatc tatcttttag tgttccatat
     3781 gcagtttggc tcttagttgg atttttccaa acagttccaa ttggaattga agaagcggct
     3841 agaattgatg gtgcaaataa atttgttacg ttttataaag ttgtgctacc gattgtagca
     3901 ccaggtattg tagcaacagc tatttataca tttatcaatg cttggaatga attcctgtat
     3961 gccttgattt tgattaacaa tacaggaaag atgacagtag cagtagccct tcgttcactt
     4021 aatggttcag aaatactaga ctggggagat atgatggcag cgtctgttat tgtagttctt
     4081 ccatcaatta ttttcttctc tatcatccaa aataagattg caagtggatt atcagaagga
     4141 tctgtgaagt agacgaaaga aggaaaaaaa atgaataaaa gaggtcttta ttcaaaacta
     4201 ggaatttctg ttgtaggcat tagtctttta atgggagttc ccactttgat tcatgcgaat
     4261 gaattaaact atggtcaact gtccatatct cctatttttc aaggaggttc atatcaactg
     4321 aacaataaga gtatagatat cagctctttg ttattagata aattgtctgg agagagtcag
     4381 acagtagtaa tgaaatttaa agcagatgaa ccaaactctc ttcaagcttt gtttggccta
     4441 tctaatagta aagcaggctt taaaaataat tacttttcaa ttttcatgag agattctggt
     4501 gagataggtg tagaaataag agacgcccaa aagggaataa attatttatt ttctagacca
     4561 gcttcattat ggggaaagca taaaggacag gcagttgaaa atacactagt atttgtatct
     4621 gattctaaag ataaaacata cacaatgtat gttaatggaa tagaagtgtt ctatgaaaca
     4681 gttgatacat ttttgccaat ttcaaatata aatggtatag ataaggcaac actaggagct
     4741 gttaatcgtg aaggtaagga acattacctc gcaaaaggaa gtattgatga aatcagtcta
     4801 tttaacaaag caattagtga tcaggaagtt tcaaatattc ccttgtcaaa tccatttcag
     4861 ttaattttcc aatcaggaga ttctactcaa gctaactatt ttagaatacc gacactatat
     4921 acattaagta gtggaagagt tctatcaagt attgatgcac gttatggtgg gactcatgat
     4981 tctaaaagta agattaatat tgccacttct tatagtgatg ataatgggaa aacgtggagt
     5041 gagccaattt ttgctatgaa gtttaatgac tatgaggagc agttagttta ctggccacga
     5101 gataataaat taaagaatag tcaaattagt ggaagtgctt cattcataga ttcatccatt
     5161 gttgaagata aaaaatctgg gaaaacgata ttactagctg atgttatgcc tgcgggtatt
     5221 ggaaataata atgcaaataa agccgactca ggttttaaag aaataaatgg tcattattat
     5281 ttaaaactaa agaagaatgg agataacgat ttccgttata cagttagaga aaatggtgtc
     5341 gtttatgatg aaacaactaa taaacctaca aattatacta taaatgataa gtatgaagtt
     5401 ttggagggag gaaagtcttt aacagtcgaa caatattcgg ttgattttga tagtggctct
     5461 ttaagagaaa aacataatgg aaaacaggtc cctatgaatg ttttctacaa agattcgtta
     5521 tttaaagtga ctcctactaa ttatatagca atgacaacta gtcagaatag aggagagagt
     5581 tgggaacaat ttaagttgtt gcctccgttc ttaggagaaa aacataatgg aacttactta
     5641 tgtcccggac aaggtttagc attaaaatca agtaacagat tgatttttgc aacatatact
     5701 agtggagaac taacctatct catttctgat gatagtggtc aaacatggaa gaaatcctca
     5761 gcttcaattc cgtttaaaaa tgcaacagca gaagcacaaa tggttgaact gagagatggt
     5821 gtgattagaa cattctttag aaccactaca ggtaagatag cttatatgac tagtagagat
     5881 tctggagaaa catggtcgaa agtttcgtat attgatggaa tccaacaaac ttcatatggc
     5941 acacaagtat ctgcaattaa atactctcaa ttaattgatg gaaaagaagc agtcattttg
     6001 agtacaccaa attctagaag tggccgtaag ggaggtcaat tagttgttgg tttagtcaat
     6061 aaagaagatg atagtattga ttggaaatac cactatgata ttgatttgcc ttcgtatggt
     6121 tatgcctatt ctgcgattac agaattgcca aatcatcaca taggtgtgct gtttgaaaaa
     6181 tatgattcgt ggtcgagaaa tgaattgcat ttaagcaatg tagttcagta tatagatttg
     6241 gaaattaatg atttaacaaa ataaaggaga aaaaacatgg ttaaatacgg tgttgttgga
     6301 gcagggtatt ttggagctga attggctcgc tacatgcaaa agaatgatgg agcagagatt
     6361 actcttctct atgatccaga taatgcagag gcgattgcag aagaattggg agcaaaagta
     6421 gcaagttcct tagatgagtt ggtttctagc gatgaagtag attgtgttat cgtcgcaact
     6481 ccaaataatc ttcataagga accggttatt aaggctgcac agcatggtaa aaatgttttc
     6541 tgtgaaaaac caattgcgct ttcttatcaa gattgtcgcg agatggtaga tgcgtgtaaa
     6601 gaaaacaatg taacctttat ggcaggacat attatgaatt tctttaatgg tgttcatcat
     6661 gcaaaagaac tcattaatca aggagttatc ggagacgttc tatattgtca tacagctcgt
     6721 aatggttggg aagaacaaca accgtcagta tcatggaaaa aaattcgtga aaaatcaggt
     6781 ggtcacttgt atcaccacat ccatgaattg gattgcgttc aattccttat ggggggcatg
     6841 cctgaaactg taaccatgac aggtggaaat gtggcccatg aaggtgaaca tttcggtgat
     6901 gaagatgata tgatttttgt caatatggaa ttttctaata agcgttttgc cttgttagaa
     6961 tggggttcag cttatcgttg gggtgaacat tatgtcttaa tccaaggaag caaaggtgcc
     7021 atccgcttag acttattcaa ctgtaaagga actcttaagc tagatgggca agaaagctat
     7081 ttcttgattc acgaatcgca agaagaagat gatgatcgga ctcgtatcta tcatagtaca
     7141 gagatggatg gagcaattgc ttatggtaaa ccaggtaaac gtactccatt atggctatca
     7201 tctgtcattg ataaagaaat gcgctatctg catgagatta tggaaggagc cccagtatca
     7261 gaagaatttg caaaactttt gacaggtgaa gctgccctag aagcaattgc tactgcagat
     7321 gcttgtaccc agtctatgtt tgaagatcgc aaagtaaaat tgtcagaaat tgtaaaataa
     7381 attttggtat tctcctattt ataggtcgac ttgctcctct gaaagtactt ttagaggagc
     7441 tgtttgactt tgctagtttt tgaaactgaa atctattata ctacaaacca ttgaaagcgt
     7501 tttaatttta aggtataata atctcataga aataaagaaa aggaggaaag aggatgccac
     7561 agattagcaa agaagccttg attgagcaaa tcaaagatgg aatcatcgtt tcttgtcagg
     7621 ctcttcctca tgaaccgctt tatacagaag cgggaggggt gattcccttg ctggtcaaag
     7681 cggctgagca aggtggagca gtcggtatcc gagcaaacag tgttcgcgat atcaaggaaa
     7741 ttaaggaagt cactaaactt ccaatcattg ggattatcaa acgtgattat ccacctcagg
     7801 aacccttcat cacggctact atgaaagaag ttgatgaatt ggcagaactg gacatcgagg
     7861 tgattgctct ggattgtacc aagcgtgaac gctacgatgg tttggaaatt caagagttca
     7921 ttcgtcaggt taaggagaaa tatcctaatc agcttttgat ggctgatact agtatcttcg
     7981 aagaagggct agcagctgta gaagcaggaa ttgactttgt cggaacaacc ttatcaggct
     8041 acacatccta cagtccaaaa gtagacggtc cagattttga attgattaag aaactctgtg
     8101 atgccggtgt agatgtcatt gcagaaggaa aaattcatac accagaacaa gccaaacaaa
     8161 tccttgaata tggagtgcga ggcatcgttg ttggtggcgc cattactaga ccaaaagaga
     8221 ttacagaacg cttcgttgct agtcttaaat aagatgtgag agggagtttt atgtttaaag
     8281 ttttacaaaa agttggaaaa gcttttatgt tacctatagc tatacttcct gcagcaggtc
     8341 tacttttggg gattggtggt gcactttcaa acccaaccac gatagcaact tatccaatac
     8401 tagacaatag tatttttcaa tcaatattcc aagtaatgag ctctgcagga gaggttgtat
     8461 tcagtaattt gtcactactt ctctgtgtgg gattatgtat tggcttagcg aaacgagata
     8521 aaggaaccgc tgcgttagca ggagtaactg gttacttagt tatgactgca acgatcaaag
     8581 ctttggtaaa actttttatg gcagaaggat ctgcaattga tactggagtt attggagcat
     8641 tagttgtcgg aatagttgcc gtatatttgc acaaccgata taacaatatt caattacctt
     8701 ccgctttagg attctttgga ggttcacgct tcgttcctat tgttacatcg ttctcttcta
     8761 tcttgattgg ctttgtcttc tttgttattt ggccaccttt ccaacaactt cttgtttcta
     8821 caggtggata tatttctcag gcgggtccaa ttggaacttt tctatatgga tttttaatga
     8881 gactttctgg agcagtaggc ttacatcata taatttaccc tatgttttgg tatactgaac
     8941 ttggtggtgt tgaaactgtt gcaggacaaa cagtggttgg agctcaaaaa atattttttg
     9001 ctcaattagc cgatccggcc cattctggat tatttacaga aggaacaagg ttttttgcag
     9061 gtcgtttctc aacaatgatg ttcggtttac cggctgcctg tttagcgatg taccatagtg
     9121 ttcctaaaaa tcgtcgtaaa aaatacgcgg gtttgttttt tggagttgct ttaacatctt
     9181 ttattaccgg tattacagaa ccaattgaat ttatgtttct attcgtcagt ccggttctat
     9241 atgttgttca cgcattcctt gatggtgtta gcttctttat tgcagacgtc ttaaatattt
     9301 caataggaaa cacattttca ggaggtgtaa tcgatttcac tttatttgga attttgcagg
     9361 ggaacgctaa gacgaattgg gttcttcaga ttccaattgg acttatttgg agtgttttgt
     9421 attatattat ttttagatgg ttcattactc aattcaacgt tctaacgcca ggacgaggag
     9481 aagaagtaga ttctaaagaa atttctgaat ccgcagattc aacttcaaat actgcagatt
     9541 atttaaaaca ggatagccta caaattatca gagccttggg tggatcaaat aatatagaag
     9601 atgtagatgc ttgtgtgaca cgtttacgtg tagctgtaaa agaagttaat caagttgata
     9661 aagcactttt aaaacaaatt ggtgcagttg atgtcttaga agtgaagggt ggcattcaag
     9721 caatctatgg agcaaaagca atcttatata aaaatagtat taatgaaatt ttaggtgtag
     9781 atgattaagt acttactgac ttaataaaaa acagaggaga gtgatggatg agtaggatga
     9841 aatgaaatcg tatacaagaa ataaagaact cattatccaa gttggatacg cttattacat
     9901 aggagaatac aaatgaaatt tagaaaatta gcttgtacag tacttgcggg tgctgcggtt
     9961 cttggtcttg ctgcttgtgg caattctggc ggaagtaaag atgctgccaa atcaggtggt
    10021 gacggtgcca aaacagaaat cacttggtgg gcattcccag tatttaccca agaaaaaact
    10081 ggtgacggtg ttggaactta tgaaaaatca atcatcgaag cgtttgaaaa agcaaaccca
    10141 gatataaaag tgaaattgga aaccatcgac ttcaagtcag gtcctgaaaa aatcacaaca
    10201 gccatcgaag caggaacagc tccagacgta ctctttgatg caccaggacg tatcatccaa
    10261 tacggtaaaa acggtaaatt ggctgagttg aacgacctct tcacagatga atttgttaaa
    10321 gatgtcaaca atgaaaacat cgtacaagca agtaaagctg gagacaaggc ttatatgtat
    10381 ccgattagtt ctgccccatt ctacatggca atgaacaaga aaatgttaga agatgctgga
    10441 gtagcaaacc ttgtaaaaga aggttggaca actgatgatt ttgaaaaagt attgaaagca
    10501 cttaaagaca agggttacac accaggttca ttgttcagtt ctggtcaagg gggagaccaa
    10561 ggaacacgtg cctttatctc taacctttat agcggttctg taacagatga aaaagttagc
    10621 aaatatacaa ctgatgatcc taaattcgtc aaaggtcttg aaaaagcaac tagctggatt
    10681 aaagacaatt tgatcaataa tggttcacaa tttgacggtg gggcagatat ccaaaacttt
    10741 gccaacggtc aaacatctta cacaatcctt tgggcaccag ctcaaaatgg tatccaagct
    10801 aaacttttag aagcaagtaa ggtagaagtg gtagaagtac cattcccatc agacgaaggt
    10861 aagccagctc ttgaatacct tgtaaacggg tttgcagtat tcaacaataa agacgacaag
    10921 aaagtcgctg catctaagaa attcatccag tttatcgcag atgacaagga gtggggacct
    10981 aaagacgtag ttcgtacagg tgctttccca gtccgtactt catttggaaa actttatgaa
    11041 gacaaacgca tggaaacaat cagcggctgg actcaatact actcaccata ctacaacact
    11101 attgatggat ttgctgaaat gagaacactt tggttcccaa tgttgcaatc tgtatcaaat
    11161 ggtgacgaaa aaccagcgga tgctttgaaa gccttcactg aaaaagcaaa cgaaacaatc
    11221 aaaaaagcta tgaaacaata gttcttagtt attctataaa aagtagtttt ttaaagaacc
    11281 taagagtgta tacccccttt tccctctaca cagatagtgt aagaaaaggg ggcttttgtt
    11341 taaaatgtaa gaaactgtca cgaaattaaa atgaagttct tacataagcg aatcataaaa
    11401 aatttcattt tgattttaaa acagttcaag aaagtcaaaa aattattcta tttgaaagag
    11461 aggtgccgac tgtgaaagtc aataaaatcc gtatgcggga aacagtgatt tcctacgctt
    11521 tcctagcacc agtattattc ttctttgtca tctttgtgtt ggctccgatg gtgatgggct
    11581 tcattacaag tttctttaac tactcaatga ctaaatttga gtttgtaggc ttggataact
    11641 atatccgtat gtttaaagat cctgtcttta caaaatctct gattaacaca gttattttgg
    11701 ttattggatc tgtaccagtt gttgttctat tctcactctt tgtagcatct cagacctatc
    11761 atcaaaatgt cattgccaga tccttctacc gtttcgtctt cttccttcct gttgtaacgg
    11821 gtagtgttgc cgtgacagtt gtttggaaat ggatttatga cccactatca gggattctaa
    11881 actttgtcct taagtccagc cacatcatca gccaaaacat ttcttggttg ggagataaaa
    11941 actgggcatt gatggcgatt atgattattc tcttgaccac ttcagttggt cagcccatca
    12001 tcctttatat cgctgccatg gggaatattg acaattcact ggttgaagcg gcgcgtgttg
    12061 atggtgcaac tgagtttcaa gttttttgga agattaaatg gccaagcctt cttccaacaa
    12121 ctctttatat tgcaatcatc acaacaatta actcattcca gtgtttcgcc ttgattcagc
    12181 ttttgacatc tggtggtcca aactactcaa caagtacctt gatgtactac ctttacgaaa
    12241 aagccttcca attgacagaa tacggctatg ccaacacaat tggtgtcttc ttggcagtca
    12301 tgattgctat cgtaagcttt gttcaattta aagtacttgg aaacgacgta gaatactaaa
    12361 gaaaggagac agctatgcaa tctacagaaa aaaaaccatt aacagccttt actgttattt
    12421 caacaatcat tttgctcttg ttgactgtgc tgttcatctt tccattctac tggattttga
    12481 caggggcatt caaatcacaa cctgatacaa ttgttattcc tcctcagtgg ttccctaaaa
    12541 tgccaaccat ggaaaacttc caacaactca tggtgcagaa ccctgccttg caatggatgt
    12601 ggaactcagt atttatctca ttggtaacca tgttcttagt ttgtgcaacc tcatctctag
    12661 caggttatgt attggctaaa aaacgtttct atggtcaacg cattctattt gctatcttta
    12721 tcgctgctat ggcgcttcca aaacaagttg tccttgtacc attggtacgt atcgtcaact
    12781 tcatgggaat ccacgatact ctctgggcag ttatcttgcc tttgattgga tggccattcg
    12841 gtgtcttcct catgaaacaa tttagtgaaa atattcctac agagttgctt gaatcagcta
    12901 aaatcgacgg ttgtggtgag attcgtacct tctggagtgt agccttcccg attgtgaaac
    12961 cagggtttgc agcccttgca atctttacct tcatcaatac ttggaatgac tacttcatgc
    13021 aattggtaat gttgacttca cgtaacaatt tgaccatctc acttggggtt gcgaccatgc
    13081 aggctgaaat ggcaaccaac tatggtttga ttatggcagg agctgccctt gctgctgttc
    13141 caatcgtcac agtcttcctt gtcttccaaa aatccttcac acagggtatt actatgggag
    13201 cggtcaaagg ataatactct gcgaaaatct cttcaaacta cgtcagcttc accttgccat
    13261 acttaagtat tgcctgcggt tagcttccta gtttgttctt caattttcat tgagtatagg
    13321 aaaatcaatc tatcaagata cagaagtata ttttatagat ttagagaata tagaggttat
    13381 aagtgtctac aaaatggagg gtatgcagtt actttatgaa gttttgtcag acacttataa
    13441 acttaagaat ggttttagtt aactatcaga aacgaaggaa agagtatgat ttttgacgat
    13501 ttgaaaaaca tcacctttta caaagggatt catcctaatt tagacaaggc tatcgactat
    13561 ctctaccaac atcgtaagga ttctttcgaa ttaggaaagt atgatattga tggagataaa
    13621 gtctttctag ttgttcagga aaatgtcctc aatcaagctg aaaatgatca atttgagtat
    13681 cataagaact atgcagattt gcatttgctg gtagaaggac atgaatattc gagctacggt
    13741 tcacgtatca aagacgaggc agtagcattc gacgaagcga gtgacattgg ctttgttcat
    13801 tgtcatgaac actacccact cttgttgggt tatcacaatt ttgcgatttt cttcccaggt
    13861 gagccacatc agccaaatgg ttatgcaggc atggaagaaa aggttcgaaa atatctcttt
    13921 aaaattttga ttgattaaaa ataggatgaa ttgttttttt gtaaagcttt gataatactc
    13981 taccatgaaa ttgatctttg tgaggtagag aaatgagaat aaaatattta aaaattggta
    14041 tcttcttgtc agtctgtgta tgctacaaga gctagtttct tggatggaca gggaattaca
    14101 gttgatgaga tggcttggat aattaggggc attgtgaatg cattgattgg tagatacata
    14161 aaattaggta cttatgcggc taagtatggt attagtatgg cacgctcgat cttaagtagg
    14221 gcagctgcaa ctgcagcagc aagagtagga ttatcgacca agatttctgg atggatttta
    14281 cgagtagctg tgaatgtagc tgatgtatat ggtaattttg ccaacaatat tgctgcagct
    14341 tgggatgcac atgataaaat ccctaacaat ggtcgtataa acttttaaaa tgcgagaatg
    14401 aaagcacttt gtattttttt attgaatatg ttagcttgga cagtgcttgc aatgataatt
    14461 cgtggagggc tagatggatt tgatgagcat acttggagta ctattttaat tgcttcgctg
    14521 ttcggggtat atgattataa gcccatagat aaaaatagaa aaagtccaaa agaaaaaaga
    14581 aaaaatagat ttgttcatgg tagggattta tgaaagcttt actgacaaaa aagaaaacag
    14641 tttacaaaga aaaatgatgg aggagcaaac atggcacaaa aaggagtaag ccttatcaag
    14701 gcagcatttg atacagacaa ctttctcatg cgttttagtg agaaagtctt ggacatcgtg
    14761 acagccaatc ttctttttgt cgtctcttgt ttgcccatcg tgacgattgg agtagctaaa
    14821 atcagcctct acgagaccat gttcgaagtt aagaagagca gacgggtgcc tgtttttaaa
    14881 atctatctaa gatctttcaa gcaaaatctg aaactaggtc ttcagctggg tttaatggag
    14941 ttaggaattg tgtttcttac cctttcagat ctctatcttt tctggggtca aacagctctg
    15001 cccttccaat tgctgaaagc catttgttta ggtattctga tttttcttac tatcgtgatg
    15061 ctggctagtt accctatcgc ggcacgttat gacctatctt ggaaagaagt gttacaaaaa
    15121 gggcttatct tggcaagttt taactttcct tggttcttcc tcatgttagc cattcttgtc
    15181 ctcattgtga tggttcttta tctgtctgcc ttcagtctac tcttaggtgg ctcagccttc
    15241 ctactttttg ggtttggact tcttgctttt ttacaagtgg gagtgatgga aaagctattt
    15301 gctaagtatg agtaaggggt gcaattattt ctgaaactac tttcaaaggc tccaaacgct
    15361 attctataag cgagaaacta aaatctaagt gtctataaga tattgaaagc cttttcggta
    15421 aagtgtatac taaagaagta aagaaaaatc tgctttagca gaaaataaaa ataataggag
    15481 aaaatctatg tcagatttga aaaaatacga aggtgtcatt ccagccttct acgcatgtta
    15541 tgatgatcaa ggagaagtaa gcccagaacg tacgcgtgcc ttggttcaat acttcattga
    15601 taaaggtgtt caaggtcttt atgtcaatgg ttcttctggt gaatgtatct accaaagcgt
    15661 tgaagatcgc aagttgattt tggaagaagt catggcggta gccaaaggta aattgaccat
    15721 tattgcccat gttgcttgca ataatactaa agatagtatg gaacttgctc gccatgctga
    15781 aagcttggga gtagatgcta ttgcaacgat tccaccaatt tatttccgct tgccagaata
    15841 ctcagttgcc aaatactgga acgatatcag ttctgcagct ccaaacacag actacgtgat
    15901 ttacaacatt cctcaattgg caggggttgc tttgactcca agcctttaca cagaaatatt
    15961 gaaaaatcct cgtgttatcg gtgtgaagaa ctcttctatg ccagttcaag atatccaaac
    16021 ctttgtcagc cttggtggag aagaccatat cgtctttaat ggtcctgatg agcagttcct
    16081 aggtggccgc ctcatgggtg ctaaggctgg tatcggtggt acttatggtg ctatgccaga
    16141 actcttcttg aaactcaatc agttgattgc ggataaggac ctagaaacag cgcgtgaatt
    16201 gcagtatgct atcaacgcaa tcattggtaa actcacttct gctcatggaa atatgtacgg
    16261 tgtcatcaaa gaagtcttga aaatcaatga aggcttgaat attggatctg ttcgttcacc
    16321 attgacacca gtaattgaag aagatcgtcc agttgtagaa gcggctgcag ccttgattcg
    16381 tgaaaccaag gagcgcttcc tctaatctaa aaggaggtat ttatgacata ctacgttgca
    16441 attgatatcg gtggaactaa catcaagtat ggtttggttg atcaagaggg gcaacttctt
    16501 gaatcacatg aaatgccaac agaggcgcat aagggtggac ctcatatctt acaaaagacc
    16561 aaagatatcg tagctagtta tttagaaaaa ggcccagtag caggtgttgc catatcttct
    16621 gctgggatgg tggatccgga taagggtgag attttctatg ctgggccgca aatccctaac
    16681 tacgcaggca cccagttcaa aaaggaaatc gaagaaagct ttactattcc ttgtgagatt
    16741 gaaaatgatg tcaactgtgc aggtcttgct gaggcagtat ctggttcagg caagggagca
    16801 agtgtgacac tttgcttgac cattggaacc ggtatcggtg gttgcttgat tatggatagg
    16861 aaagtcttcc atggttttag taattcagcc tgtgaagtcg ggtatatgca tatgcaggat
    16921 ggagcttttc aggatctcgc ttctacaaca gctttagtga aatatgtagc tgaagcccat
    16981 ggagaagatg ttgatcagtg gaatggccga cgtatcttta aggaagctac tgaaggaaac
    17041 aaaatctgca tggaaggtat tgaccgtatg gttgactatc taggaaaagg tctggcaaat
    17101 atttgctacg ttgccaatcc agaagtggtc attcttggtg gtggtatcat ggggcaagag
    17161 gctatcctca aacctaagat ccgcacagcc ttgaaagagg ctttggtacc aagtttagca
    17221 gaaaaaacac gattagaatt tgcccatcac caaaatacag cagggatgtt gggtgcatat
    17281 tatcatttta agacaaaaca atcctagttt ggctcagcca aactaggatt ttcttacacg
    17341 tttttgtcta cgatagccgt tgagtttttt attttcccag tagctattaa agattttttc
    17401 cttgctttcg cgattgattt ccaaaaagta ggcataaatc aaatcgataa agaagagcat
    17461 aggaagttga gcggatattc gttggatata ggagggttgg ctgtgggtgg ctacaagaac
    17521 agtctctgta taggtctggc tatctttatt gggaacactt gtaaagagta cagtctttgc
    17581 ccccatctcc ttggcatcta atagactatc taaaatagaa ggagttgagc ctgaaagtga
    17641 gaagcccagt actagacaat tttcatccat gatgctggtt gtccaggcaa agccgtcttg
    17701 gtctgtcaaa gcttcgcaga ccacacctaa tcgcataaaa cgtaatttca tttcacgggc
    17761 gacgaggcca gaactccctg ttccaaagaa gtagatacgc tcagcatctt cgattagctg
    17821 ggcaattcgt tctagttgga tttcgtcaat caagtcttgt gtttgttccc tcatattgct
    17881 ataacttctg aggactcgtt tggtcagtgg actgtgcttg gagacttggt tggcttgatt
    17941 ttctgcctga tgttggtatt ggaaaataaa ttctcggtag ccagtaaagc cacacttttt
    18001 agcaaagcgg gtcaaagcag cttgagaaat atgtaatttt tgggtgactt gttgagaaga
    18061 taaatcatct gtaatcgttt cagcttgcaa aaaatagcga gcgatttctt gctctaggtc
    18121 tgtcatttct tcaaaatgtg aatcaatgat agttgcgata tctggtttgt ccatagggaa
    18181 agctccttta catgagtcat actggaagac tagatcaggg aatagtcaca cttcattata
    18241 acacataata taaggataga taaataaaaa cgcatctctg ttttaaaaac gaaaaaatcg
    18301 aaaaagcttc tctcttttcc ataattttct actcaaattg tggtacaatt aagagtaaga
    18361 ttttaagtta gaaatgagac tgatttgtat gagaaaattt aacagccatt cgattccgat
    18421 tcggcttaat ttattgtttt caatcgtcat tttactcttt atgaccatta ttggtcgttt
    18481 gttgtatatg caggttttga acaaggattt ttacgaaaaa aagctagcct cagctagtca
    18541 gaccaagatt acaagcagtt cagcccgtgg ggaaatttat gatgctagtg gaaaaccttt
    18601 ggtagaaaat acgttaaagc aggttgtttc ctttacgcgt agcaataaaa tgacggctac
    18661 agacttaaaa gaaacagcta aaaagttact gacttatgtg agcatcagtt ctccaaattt
    18721 gacagaacgc cagctggcgg attactattt ggctgatcct gaaatctata aaaaaacagt
    18781 ggaagctctc ccaagcgaga aacgcttgga ttcagatggc aatcgtctat ctgaatcaga
    18841 actgtataac aatgcggtcg atagtgtaca aacgagtcaa ctaaactata cagaggatga
    18901 aaagaaagaa atctatcttt ttagtcagtt aaatgctgtt ggaaactttg cgacaggaac
    18961 cattgcgaca gatcctctaa atgattctca ggtggctgtt attgcctcta tttcaaagga
    19021 gctgcctggc attagtattt ctacttcttg ggatagaaag gttttggaaa cttccctttc
    19081 ttctatagtt gggagtgtat ccagtgaaaa agctggtctc ccagcggaag aagcagaagc
    19141 ctatcttaaa aaaggctatt ctctaaatga tcgtgtagga acctcctatt tggaaaagca
    19201 atatgaagag accttacaag gaaaacgctc ggtaaaagaa atccatctgg ataaatatgg
    19261 caatatggaa agcgtggata caattgagga aggtagtaag ggaaacaata tcaaactgac
    19321 cattgatttg gccttccaag atagcgtgga tgctttactg aaaagttatt tcaattccga
    19381 gctagaaaat ggtggagcca agtattctga aggtgtctat gcagtcgccc ttaacccaaa
    19441 aacaggtgcg gttttgtcta tgtcagggat taaacatgac ttgaaaacgg gagagttgac
    19501 gcctgattcc ttgggaacgg taaccaatgt ctttgttcca ggttcggttg tcaaggcggc
    19561 gaccatcagc tcaggttggg aaaatggagt cttgtcagga aaccagacct tgacagacca
    19621 gtccattgtc ttccaaggtt cagctcccat caattcttgg tatactcagg cttacggttc
    19681 attccctatc acagcggtcc aagctctgga gtattcatca aatacctata tggtccaaac
    19741 agccttaggt cttatggggc aaacctatca acccaatatg tttgtcggca ccagcaatct
    19801 agagtctgct atggagaaat tgcgttcaac ctttggcgaa tatggcttgg gtactgcgac
    19861 aggaattgac ctaccagatg aatctactgg atttgttccc aaagagtata gctttgctaa
    19921 ttacattact aatgcctttg ggcagtttga taactatacg ccgatgcagt tggctcagta
    19981 tgtagcaact attgcaaata atggtgttcg tgtggctcct cgtattgttg aaggcattta
    20041 tggtaataat gataagggag gactgggtga cttgattcag caactgcaac cgacagagat
    20101 gaataaggtc aatatatccg actccgatat gagcatcttg caccaaggtt tttatcaggt
    20161 tgcccatggt actagtggat tgacaactgg acgtgccttt tcaaatggtg ccttggtatc
    20221 cattagcgga aaaacaggta cagccgaaag ctatgtggca gatggtcagc aagcaaccaa
    20281 taccaatgcg gtggcctatg ccccatctga taatccccaa atcgctgtcg cagtggtctt
    20341 tcctcataat accaatctaa caaatggtgt aggaccttcc attgcgcgtg acattatcaa
    20401 tctgtatcaa aaataccatc caatgaacta gaaaggaaat tatgctttat ccaacaccta
    20461 ttgccaagtt gattgacagt tattctaagt taccaggtat cgggattaag acggctacgc
    20521 gtctggcctt ttatacgatt gggatgtctg ctgatgatgt caatgaattt gcaaaaaatc
    20581 tcctttctgc taagagagaa ttgacatatt gttctatttg tggacgtttg acagacgacg
    20641 atccttgttc tatctgtact gatccgactc gtgaccagac aacaatttta gttcttgagg
    20701 atagtagaga tgtggcagcc atggaaaata tccaagaata ccatggactc tatcatgtcc
    20761 tgcatggcct catttctcct atgaatggta tcagtccgga cgatatcaat ctcaagagcc
    20821 ttatgactcg tcttatggat agtgaggttt cagaagtgat tgtggcgact aatgctacag
    20881 cggatggtga agcgacttcc atgtatcttt cacgtttgct caagccggct ggtatcaagg
    20941 ttacgcgtct agcacgaggt ctcgctgtgg gagcggacat tgagtatgcg gacgaagtga
    21001 cactcttacg agcgattgaa aatcggacag agttgtaagt gtaggcaaat ttacgaactc
    21061 ccttcattta taaaaaatca aagaggctga aaatcgttcc tatcggcctc tttttgtata
    21121 gtgtgatgag taggatcggg ttcaagtttt aaaaaaccaa gcaaatatga tatactaaag
    21181 agcgagtatt ctagtagaat taggacaaat aatatgaaac aaacgattat tcttttatat
    21241 ggtggacgga gtgcggaacg cgaagtctct gtcctttcag ctgagagtgt catgcgtgcg
    21301 gtcaattacg accgtttcac agtcaagact ttctttatca gtcagtcagg tgactttatc
    21361 aaaacacagg aatttagtca tgctccgggg caagaagacc gtctcatgac caatgaaacc
    21421 attgattggg ataagaaagt tgcaccaagt gctatctacg aagaaggtgc agtggtcttt
    21481 ccagtccttc acgggccaat gggagaagat ggctctgttc aaggattctt ggaagttttg
    21541 aaaatgcctt acgttggttg caacattttg tcatcaagtc ttgccatgga taaaatcacg
    21601 actaagcgtg ttctggaatc tgctggtatt gcccaagttc cttatgtggc tatcgttgaa
    21661 ggcgatgatg tgactgctaa aatcgctgaa gtggaagaaa aattggctta tccagtcttc
    21721 actaagccgt caaacatggg gtctagtgtc ggtatttcta agtctgaaaa ccaagaagaa
    21781 ctccgtcaag ccttaaaact tgccttccga tatgacagcc gtgtcttggt tgagcaagga
    21841 gtgaatgccc gtgaaattga ggttggcctc ttgggtaact acgatgtcaa gagcacgcta
    21901 cctggagaag ttgtcaagga cgttgccttt tatgactacg atgccaagta tattgataac
    21961 aagattacta tggatattcc tgccaaaatc agtgatgatg tggtggctgt catgcgtcaa
    22021 aatgcagaaa cagccttccg tgccattggt ggccttggtc tatctcgttg cgatttcttc
    22081 tatacagata agggagagat ttttctcaac gagctcaata ccatgccagg ttttacccag
    22141 tggtctatgt acccactact ttgggacaat atggggatca gctacccagg actaatcgag
    22201 cgtttggttg accttgccaa ggaaagcttt gacaagcgcg aagcgcattt gatataaaat
    22261 gaaagagagg gtagaagcca gaaccatcac tgcacggtga ctagagttct cggacttcag
    22321 ccctttttaa aggagtagaa atgaaattaa caatccatga aattgcccaa gttgttggag
    22381 ccaaaaatga tatcagtatc tttgaggaca cccagttaga aaaagctgag tttgatagtc
    22441 gtttgattgg aactggagat ttatttgtgc cacttaaagg tgcgcgtgat ggccatgact
    22501 ttattgaaac agcctttgaa aatggtgcag cagtaacctt gtctgagaaa gaggtctcaa
    22561 atcatcctta cattttagta gatgatgttt tgacagcctt tcaatcccta gcatcctact
    22621 atcttgaaaa aacgactgtt gatgtctttg ctgttacagg ttcaaatggc aagacaacga
    22681 ctaaggatat gttggcgcat ttactgtcaa caagatacaa gacctacaaa acacaaggca
    22741 attacaataa tgagattggc cttccttaca cagttctcca catgcctgaa ggaacagaaa
    22801 agttggtctt ggagatggga caggatcact tgggcgatat tcatctcttg tctgaattgg
    22861 ctcgtccaaa aacagccatc gtgaccttgg ttggagaagc ccatttggcc tttttcaaag
    22921 accgttcaga gattgctaag ggaaaaatgc aaattgcaga cggaatggct tcaggttcct
    22981 tgcttttagc gccggctgac cctatcgtag aggactattt gccaactgat aaaaaggtgg
    23041 ttcgttttgg gcaaggggca gagctggaaa ttactgactt ggttgagcgc aaagatagtc
    23101 tgaccttcaa ggccaatttc ttagagcaag cccttgattt gccagtaact ggcaagtaca
    23161 atgcgacaaa tgctatgatt gcatcctatg ttgccttgca agaaggagtt tcagaggagc
    23221 aaattcgttt ggccttccaa gatcttgaat tgacgcgtaa ccgtaccgag tggaagaaag
    23281 cagccaatgg agcagatatc ctgtcagatg tttacaatgc caatccaact gctatgaaac
    23341 tgattttaga gactttctct gccattccag ccaatgaagg tggcaagaaa attgcagtgt
    23401 tggcggatat gaaggagctt ggtgaccagt ctgttcaact tcataatcag atgattttga
    23461 gcctttctcc agatgtgctt gataccgtga ttttctatgg acaagatatt gctcagttgg
    23521 ctcaattggc cagtcaaatg ttcccaatcg gccacgttta ctacttcaag aaaacagaag
    23581 accaggatca atttgaagac ctagtcaagc aggtcaagga aagccttgga gcccatgacc
    23641 aaatcctgct caaaggctct aactctatga atctagccaa gttggtagaa agtttagaaa
    23701 atgaagacaa gtgattttgt caagtatttg caaagaatga ttgccattac agatactggc
    23761 ttaaccttta caaaagatcc gtttgaccgt gagcgctacg aagacttgcg aagtctgtta
    23821 tctgaaatgt tgaatcaagc atcagacctt gattccgaag aagtggcaga agtcttgaag
    23881 ccaacttctg cttatgcgac tcccttaatg gacgtccgtg cttggattgt tgaggatgag
    23941 aagatttgtc tggttagggg acaaggagag gatagttggg ctttgccagg tggctttggt
    24001 gaagtcggct attctccaac agaaaatatt ctcaaggaaa ttgaagaaga aaccggtttt
    24061 aaagccaaag ttgaaagact gctagctgtt tttgatacca atcgtttcca actacagagc
    24121 aaacagtata caaagtttgt ttttggatgt aagcttcttg atggacaatt ccaagaaaat
    24181 caagaaattg ctgaccttca attttttgcc attgaccaac tgccgaactt atctgaaaaa
    24241 cgcattacca aggagcaaat agagcttctt tggcaggttt atcaaggtca tagggggcaa
    24301 tatcttgact aagaagatga ttatcgtatt tctaaatcca tttttaacaa ctagcatggt
    24361 ataataatat gcaggaaaat tttgaattat gaggaagact agatgaattt atgggatatt
    24421 ttctttacga ctcaggcaac cgagccgccc aaatttgacc ttttttggta tgttagccta
    24481 tttacgctct tagccttaac cttttataca gcccatcgct atcgtgaaaa gaaggtttac
    24541 caacgatttt tccaaatctt gcagactgtt cagttaatcc ttctttatgg ttggtactgg
    24601 gtcaatcata tgccactgtc agaaagccta cccttttacc attgccgtat ggctatgttt
    24661 gtggtactct tgcttcctgg tcaatccaaa tataaacaat actttgcatt attgggaaca
    24721 tttgggacat tagcagcctt tgtttatcca gtgccagatg cttacccttt tccacatatc
    24781 accattctat cctttatctt tggtcattta gcactcttgg ggaactctct agtttatcta
    24841 ttgagacagt ataatgcgcg attgctggat gtgaagggaa tttttctcat gacctttgcc
    24901 ctaaatgcct tgatttttgt ggtcaatttg gtgacaggtg gcgattacgg atttttgaca
    24961 aaaccgccat tggttgggga tcacggtcta gtagctaatt atttacttgt ttcaattgtg
    25021 ctggtagcta ctatcagttt gactaagaaa atcttagaat tctttttagc tcaagaagca
    25081 gaaaaaatga ttgcaaagga agcttaacac agagctttct tttttgctct tagagagttt
    25141 ttacaagcag cttataaaat aagaatttct gaatagacaa actcaaaaaa tggctgggaa
    25201 atttaggaaa aaagcaagca cgattaaatt ttttgtgtta taatattttg tgaatagcta
    25261 tgcctatgtt tagctatgga ataatacgaa gtgcgaaact tggaagatag agaggaagcg
    25321 atgtaatggc tagagaaggc ttttttacag gtctagatat tggaacaagc tctgtcaagg
    25381 tgcttgtggc cgagcagaga aatggtgaat taaatgtaat tggcgtgagt aatgccaaaa
    25441 gtaaaggtgt aaaggatgga attattgttg atattgatgc agcagcaact gctatcaagt
    25501 cagccatttc ccaagcggaa gaaaaggcag gcatttcgat taaatcagtg aatgtcggct
    25561 tgcctggtaa tcttttgcag gtagaaccaa ctcaggggat gattccagta acatctgata
    25621 ctaaggaaat tacggatcaa gatgttgaaa atgttgtcaa atcagctttg acaaagagta
    25681 tgacacctga ccgtgaagtc attaccttta ttcctgaaga atttattgtg gatggtttcc
    25741 aagggattcg tgacccacgt ggcatgatgg gggttcgcct tgaaatgcgt ggtttgcttt
    25801 atacaggacc tcgtactatc ttgcacaatt tgcgtaagac ggttgagcgt gcaggtgttc
    25861 aggttgaaaa tgttatcatt tcaccactag caatggttca gtctgttttg aacgaagggg
    25921 aacgtgaatt tggtgctaca gtgattgata tgggggcagg tcaaacgact gtcgctacaa
    25981 tccgtaatca agaactccag ttcacacata ttctccaaga aggtggagat tatgtaacta
    26041 aagatatctc caaggttttg aaaacctctc gcaaattagc ggaaggcttg aaactgaatt
    26101 acggggaagc ctatccgcct cttgcaagca aagaaacctt ccaagtagag gttattggag
    26161 aagtagaagc agtcgaagtg acggaagcct acttgtcaga aattatttct gcacgaatca
    26221 agcacatcct tgaacaaatc aagcaagaat tagatagaag gcgtctattg gacctccctg
    26281 gtggtattgt cttaatcggt gggaatgcca ttttaccagg tatggttgag cttgctcagg
    26341 aagtctttgg cgtccgtgtc aagctttatg ttccaaatca agttggtatc cgtaatccag
    26401 cctttgcgca tgtgattagt ttatcagaat ttgcgggtca attaacagaa gttaatcttt
    26461 tggctcaggg agcgataaaa ggtgagaatg acttaagtca tcagccaatt agttttggtg
    26521 ggatgctgca aaaaacagct cagtttgtac aatcaacgcc tgttcaacca gctcctgctc
    26581 cagaagtaga gccggtggcg cctacagaac caatggcgga tttccaacaa gcttcacaaa
    26641 ataaaccgaa attagcagat cgtttccgtg gattgatcgg aagcatgttt gacgaataaa
    26701 gaggaaaaat aaattatgac attttcattt gatacagctg ctgctcaagg ggcagtgatt
    26761 aaagtaattg gtgtcggtgg aggtggtggc aatgccatca accgtatggt cgacgaaggt
    26821 gttacaggcg tagaatttat cgcagcaaac acagatgtac aagcattgag tagtacaaaa
    26881 gctgagactg ttattcagtt gggacctaaa ttgactcgtg gtttgggtgc aggaggtcaa
    26941 cctgaggttg gtcgtaaagc cgctgaagaa agcgaagaaa cactgacgga agctattagt
    27001 ggtgccgata tggtcttcat cactgctggt atgggaggag gctctggaac tggagctgct
    27061 cctgttattg ctcgtatcgc caaagattta ggtgcgctta cagttggtgt tgtaacacgt
    27121 ccctttggtt ttgaaggaag taagcgtgga caatttgctg tagaaggaat caatcaactt
    27181 cgtgagcatg tagacactct attgattatc tcaaacaaca atttgcttga aattgttgat
    27241 aagaaaacac cgcttttgga ggctcttagc gaagcggata acgttcttcg tcaaggtgtt
    27301 caagggatta ccgatttgat taccaatcca ggattgatta accttgactt tgccgatgtg
    27361 aaaacggtaa tggcaaacaa agggaatgct cttatgggta ttggtatcgg tagtggagaa
    27421 gaacgtgtgg tagaagcggc acgtaaggca atctattcac cacttcttga aacaactatt
    27481 gacggtgctg aggatgttat cgtcaacgtt actggtggtc ttgacttaac cttgattgag
    27541 gcagaagagg cttcacaaat tgtgaaccag gcagcaggtc aaggagtgaa catctggctc
    27601 ggtacttcaa ttgatgaaag tatgcgtgat gaaattcgtg taacagttgt tgcaacgggt
    27661 gttcgtcaag accgcgtaga aaaggttgtg gctccacaag ctagatctgc tactaactac
    27721 cgtgagacag tgaaaccagc tcattcacat ggctttgatc gtcattttga tatggcagaa
    27781 acagttgaat tgccaaaaca aaatccacgt cgtttggaac caactcaggc atctgctttt
    27841 ggtgattggg atcttcgccg tgaatcgatt gttcgtacaa cagattcagt cgtttctcca
    27901 gtcgagcgct ttgaagcccc aatttcacaa gatgaagatg aattggatac acctccattt
    27961 ttcaaaaatc gttaagtaaa tgaatgtaaa agaaaataca gaacttgttt ttcgagaagt
    28021 tgcagaggct agtctgagtg ctcatcgaga gagtggttcg gtctctgtca ttgcagttac
    28081 caagtatgta gatgtaccga cagcggaagc cttgcttccg ctaggtgtcc atcatatcgg
    28141 tgaaaatcgt gtagataagt ttctggaaaa atatgaagct ttaaaagatc gagatgtgac
    28201 ttggcatttg attggtacct tgcaaagacg taaggtgaaa gatgtcattc aatacgttga
    28261 ttatttccat gcattggact cagtaaagtt agcaggggaa attcaaaaaa gaagtgaccg
    28321 agtcatcaag tgtttccttc aagtaaatat ttctaaagaa gaaagcaaac acggtttttc
    28381 gagagaggaa ctgctggaaa tcttgccaga gttagccaga ctagataaga ttgaatatgt
    28441 tggtttaatg acgatggcac cttttgaggc tagcagtgag cagttgaaag agattttcaa
    28501 ggcggcccaa gatttacaaa gagaaattca agagaaacaa attccaaata tgcctatgac
    28561 cgagttaagt atgggaatga gtcgtgatta taaagaagcg attcaattcg gttccacttt
    28621 tgttcgtata ggtacatcat tttttaagta ggagagaacc atgtctttaa aagatagatt
    28681 cgatagattt atagattatt ttacggagga tgaggattca agtctccctt atgaaaaaag
    28741 agatgagcct gtgtttactt cagtaaattc ttcacaggaa ccggctctcc caatgaatca
    28801 accttcacag tcggctggca caaaagagaa caatatcacc agacttcatg caagacaaca
    28861 ggaattggca aatcagagtc agcgtgcaac ggataaggtc attatagatg ttcgttatcc
    28921 tagaaaatat gaggatgcaa cagaaattgt tgatttattg gcaggaaacg aaagtatctt
    28981 gattgatttt cagtatatga cagaggtgca ggctcgtcgt tgtttggact atttggatgg
    29041 agcttgtcat gttttagctg gaaatttgaa aaaggtagct tctaccatgt atttgttgac
    29101 accagtgaac gttattgtaa atgttgaaga tatccgttta ccagatgaag atcaacaggg
    29161 tgagttcggt tttgatatga agcgaaatag agtacgataa tgattttttt aattcgtatg
    29221 atttataatg cagtggatat ttactcccta attttggtag ccttcgctgt catgtcttgg
    29281 tttccaggtg cctacgaatc cagtttaggt cgttggattg tagcgttggt aaaaccagtg
    29341 cttgctccct tgcaacgcct gcctttacag atagcgggtc ttgatttatc tgtttgggtt
    29401 gcgattgttt tggttcgatt tttaggagaa aacctagtgc gttttctggc gatgatagga
    29461 tgaataaagg gatttatcag catttctcca tagaagatcg tccatttctt gacaagggaa
    29521 tggaatggat aaagaaggta gaagatagct atgctccttt tttaactcct tttatcaatc
    29581 ctcatcagga gaagctatta aagattttgg ccaaaaccta tggtcttgct tgtagcagta
    29641 gtggggaatt cgtctcgagt gagtatgttc gagttttatt atacccagat tatttccaac
    29701 cagagttttc agattttgaa atatctctcc aggaaattgt gtattccaat aaatttgaat
    29761 atttaacgca tgctaagatt ttagggacag tcatcaatca attagggatt gaacggaaac
    29821 tttttggaga tatcctagta gatgaagaac gggcgcagat tatgattaat cagcagtttc
    29881 ttctcctctt tcaagatgga ttaaagaaaa ttggtcgtat acctgtttcg ctggaggaac
    29941 gtcctttcac cgagaaaata gataagctag aacagtatcg agaactggat ttatctgtgt
    30001 ctagttttcg attagatgtt cttttatcaa atgttttgaa actatctagg aatcaagcaa
    30061 accagttgat tgaaaagaaa cttgtccaag taaattatca tgtggtagac aaatcagatt
    30121 acactgttca agttggagac ttgattagtg tgagaaaatt tggtcgcttg agattacttc
    30181 aagataaggg acaaacgaaa aaagagaaga aaaaaataac cgtccagtta ttattaagta
    30241 agtgaggaat agaatgccaa ttacatcatt agaaataaag gacaagactt ttggaactcg
    30301 attcagaggt tttgatccag aagaagtcga tgaattttta gatattgtgg ttcgtgatta
    30361 cgaagatctt gtgcgtgcga atcatgataa aaatttgcgt attaagagtt tagaagagcg
    30421 tttgtcttac tttgatgaaa taaaagattc attgagccag tctgtattga ttgcccagga
    30481 tacagctgag cgagtgaaac aggcggcgca tgaacgttca aacaatatca ttcatcaagc
    30541 agagcaagat gcgcaacgct tgttggaaga agctaaatat aaggcaaacg agattcttcg
    30601 tcaagcaact gataatgcta agaaagtcgc tgttgaaaca gaagaattga agaacaagag
    30661 ccgtgtcttc caccaacgtc tcaaatctac aattgagagt cagttggcta ttgttgaatc
    30721 ttcagattgg gaagatattc tccgtccaac agctacttat cttcaaacca gtgatgaagc
    30781 ctttaaagaa gtggttagcg aagtacttgg agaaccgatt ccagctccaa ttgaagaaga
    30841 accaattgat atgacacgtc agttctctca agcagaaatg gaagaattac aagctcgtat
    30901 tgaggtagcc gataaagaat tgtctgaatt tgaagctcag attaaacagg aagtggaaac
    30961 tccaactcct gtagtgagtc ctcaagttga agaagagcct ctgctcatcc agttggccca
    31021 atgtatgaag aaccagaagt agctccagtg catccgacag gtccaacacc agctacagaa
    31081 actgttgatt cagcaccggg atttgaagca ccgcaagaat ctgttacaat tttataagaa
    31141 atattctgag aacaatatct tatccttata ttcccagcga gcaggagatg gtgtgagtcc
    31201 tgcattccct atcgataaga ttatcctctc aaactatcaa gtctgaatct agtaagattt
    31261 gacgttcccc acgttacggg ataagaggga gaaagactaa aactttttcc gaataaaggt
    31321 ggtaccacga ttttcgtcct ttttggaagt cgtggttttt aatttgttat tatttataaa
    31381 ggagatacca tgaaactcaa agacaccctt aatcttggga aaactgaatt cccaatgcgt
    31441 gcaggccttc ctaccaaaga gccagtttgg caaaaggaat gggaagatgc aaaactttat
    31501 caacgtcgtc aagaattgaa ccaaggaaaa cctcatttca ccttgcatga tggccctcca
    31561 tacgctaacg gaaatatcca cgttggacat gctatgaaca agatttcaaa agatattatt
    31621 gttcgttcta agtctatgtc aggattttac gcaccattta ttcctggttg ggatactcat
    31681 ggtctgccaa tcgagcaagt attgtcaaaa caaggtgtca aacgtaaaga aatggacttg
    31741 gttgagtact tgaaactttg ccgtgagtac gctctttctc aagtagataa acaacgtgaa
    31801 gattttaaac gtttgggtgt ttctggtgac tgggaaaatc catatgtgac cttgactcct
    31861 gactatgaag cagctcaaat tcgtgtattt ggtgagatgg ctaataaggg ttatatctac
    31921 cgtggtgcca agccagttta ctggtcatgg tcatctgagt cagcccttgc tgaagcagag
    31981 attgaatacc atgacttggt ttcaacttcc ctttactatg ccaacaaggt aaaagatggc
    32041 aaaggagttc tagatacaga tacttatatc gttgtctgga caacgactcc atttaccatc
    32101 acagcttctc gtggtttgac ggttggtgca gatattgatt acgttttggt tcaacctgtt
    32161 ggtgaagctc gtaagtttgt cgttgctgct gaattattga ctagcttgtc tgagaaattt
    32221 ggctgggctg atgttcaagt tttggaaact taccgtggcc aagaactcaa ccacatcgta
    32281 acagaacacc catgggatac agctgtagaa gagttggtaa ttcttggtga ccacgttacg
    32341 actgactctg gtacaggtat tgtccataca gcccctggtt ttggtgagga cgattacaat
    32401 gttggtattg ctaataatct tgaagtcgca gtgactgttg atgaacgtgg tatcatgatg
    32461 aagaatgctg gtcctgaatt tgaaggtcaa ttctatgaaa aggtagttcc aactgttatt
    32521 gaaaaacttg gtaacctcct tcttgcccaa gaagaaatct ctcactcata tccatttgac
    32581 tggcgtacta agaaaccaat catctggcgt gcagttccac aatggtttgc ctcagtttct
    32641 aaattccgtc aagaaatctt ggacgaaatt gaaaaagtga aattccactc agaatggggt
    32701 aaagtccgtc tttacaatat gatccgtgac cgtggtgact gggttatctc tcgtcaacgt
    32761 gcttggggtg ttccacttcc tatcttctac gctgaagatg gtacagctat catggtagct
    32821 gaaactattg aacacgtagc tcaacttttt gaagaacatg gttcaagcat ttggtgggaa
    32881 cgtgatgcca aagacctctt gccagaagga tttactcatc caggttcacc aaacggcgag
    32941 ttcaaaaaag aaactgatat catggacgtt tggtttgact caggttcatc atggaatgga
    33001 gtggtggtaa accgtcctga attgacttac ccagccgacc tttacctaga aggttctgac
    33061 caataccgtg gttggtttaa ctcatcactt atcacatctg ttgccaacca tggcgtagca
    33121 ccttacaaac aaatcttgtc acaaggtttt gcccttgatg gtaaaggtga gaagatgtct
    33181 aaatctcttg gaaataccat tgctccaagc gatgttgaaa aacaattcgg tgctgaaatc
    33241 ttgcgtctct gggtaacaag tgttgactca agcaatgacg tgcgtatctc tatggatatt
    33301 ttgagccaag tttctgaaac ttaccgtaag attcgtaaca ctcttcgttt cttgattgcc
    33361 aatacatctg actttaaccc agctcaagat acagtcgctt acgatgagct tcgttcagtt
    33421 gataagtaca tgacgatttg ctttaaccag cttgtcaaga ccattcgtga tgcctatgca
    33481 gactttgaat tcttgacgat ctacaaggcc ttggtgaact ttatcaacgt tgacttgtca
    33541 gccttctacc ttgattttgc caaagatgtt gtttacattg aaggtgccaa atcactggaa
    33601 cgccgtcaaa tgcagactgt cttctatgac attcttgtca aaatcaccaa actcttgaca
    33661 ccaatccttc ctcatactgc ggaagaaatt tggtcatatc ttgagtttga aacagaagac
    33721 ttcgtccaat tgtcagaatt accagaggct caaacttttg ctaatcaaga agaaatcttg
    33781 gatacatggg cagccttcat ggacttccgt ggacaagctc aaaaagcctt ggaagaagct
    33841 cgtaatgcaa aagttatcgg taaatcactt gaagcacact tgacagttta tccaaatgaa
    33901 gtggtgaaaa ctctactcga agcagtaaac agcaatgtag ctcaactttt gatcgtgtct
    33961 gagttgacca tcgcagaagg accagctccg gaagctgccc ttagcttcga agatgtagcc
    34021 ttcacagttg aacgtgctgc tggtgaagta tgtgaccgtt gccgtcgtat tgacccaaca
    34081 acagcagaac gcagctacca ggcagttatc tgtgaccact gtgcaagcat cgtagaagaa
    34141 aactttgcgg aagcagtcgc agaaggattt gaagagaaat aagattgaaa agtctaggca
    34201 aaattcaatt tgagaagaaa agacaactaa ttttatagtc tattaaacgc attgtatcac
    34261 gtttttgaac acctgatatg atgcgttttt tatttatttt aaaaatttgc gaggtatgac
    34321 tttttatact caacaagaat caaagagaaa cttagcaagc taacagtagt aagataaaat
    34381 aggaatttga tattagggat aagattggta aatagtgtaa tatttttaca acaataaatt
    34441 tatatagtta tttctggttt ctgaaaagta ttatattata ttttatttca tattatacaa
    34501 atttttattt tataatatca gaaaatactt tttttaaaaa gcaaatatga tacaatttta
    34561 tttgaaaaaa aataaaaaag gagattttat tatgaaatca agtggtatcg tcggtcttgt
    34621 gggaggaatt ttacttctta tttgtccttt tgtcttgttg ggaacagcgg taaatacagc
    34681 tgctacaact cttaatggag gagctactgc aggggctttt tcaggtgttg ccttactctt
    34741 gaatgccttg aagattgcaa atcttgttct tggtatcatt gctattgttt actataaagg
    34801 agataagcat gtaggtgcag ctccgtctgt actaatgatt gtttctggtg aagttagtct
    34861 cattccattc ttaggatggg ttgggaggat tcttgctatt atcggaggat ctctattcct
    34921 tgcaacattg aagaaattca aatcagaaga ataaaaggta ttttagcatg aaaagaacaa
    34981 aaaagtttat cggtatagga gtagctctat tatctctttc tcttctagtt gcatgtggaa
    35041 cataaagttc aaagaatact tcaacaagta atgatgagaa gacagtagca acatccaata
    35101 gttcaaaaga aacaatcact ttcgatacac cggttgtaac agacgatgcg attgaatcaa
    35161 tacgcactta tgcagattat atagatcttt ataaaaatat ttttgatgat tattttacta
    35221 aagctgagga aggtttcaaa ggcacaacta tggaaaataa tgactcgttt actaaactaa
    35281 aagagtcaac tcaaaaatta ttcgatgcgc agaaaaaaag gttaaataat gaagatagaa
    35341 tagaaacaac caaaaacaat gtgattgcca aacattgtca aacagtcctt tcctttttgg
    35401 ttttgactag cttttttgtg aaaaattgtg taaaatagaa tagataaacg aggggaaacc
    35461 tcggaaaatt taaaggagaa tccatctaat ggtaaaattg gtttttgctc gccacggtga
    35521 gtctgaatgg aacaaagcta accttttcac tggttgggct gatgttgatt tgtctgaaaa
    35581 aggtacacaa caagcgattg acgctggtaa attgatcaaa gaagctggta tcgaatttga
    35641 ccaagcttac acttcagtat tgaaacgtgc tatcaaaaca actaacttgg ctcttgaagc
    35701 ttctgaccaa ttatgggttc cagttgaaaa atcatggcgc ttgaacgaac gtcactacgg
    35761 tggtttgact ggtaaaaaca aagctgaagc tgctgaacaa tttggtgatg agcaagttca
    35821 catctggcgt cgttcatacg atgtattgcc tccaaatatg gaccgtgatg atgagcactc
    35881 agctcacaca gaccgtcgtt atgcttcact tgacgactca gtaatcccag atgctgaaaa
    35941 cttgaaagtg actttggaac gtgctcttcc attctgggaa gataaaatcg ctccagctct
    36001 taaagatggt aaaaacgtat tcgtaggagc tcacggtaac tcaatccgtg cccttgtaaa
    36061 acacatcaaa ggtttgtcag atgatgagat catggacgtg gaaatcccta acttcccacc
    36121 attggtattc gaattcgatg aaaaattgaa cgttgtttct gaatactacc ttggaaaata
    36181 aaaaattgta agcctaggat tgatttctag gctttttatg ttagtatgga agtatgataa
    36241 ggaataaaaa acaagattat gtactggcct acaagcaacc agcttcaacc acttacatgg
    36301 gttgggaaga agaagcttta ccgataggta atggttcttt aggagcaaaa gtatttggcc
    36361 ttatagggtc tgaacggatt caatttaatg aaaaaagtct ctggtctgga ggtccacttc
    36421 ctgatagttc agattatcag ggtggaaatc ttcaggatca gtatgttttt ttagctgaga
    36481 ttcggcaggc tttggagaag agagattaca atctggctaa ggaactggct gagcagcacc
    36541 taattgggcc aaaaacgagt caatatggga cctatctgtc ttttggggat attcacattg
    36601 agttcagcca gcaaggtacg actttgtctc aggtgacgga ctatcagaga cagttgaata
    36661 ttagtaaggc acttgcgacg acttcttatg tctataaggg aacgcgattt gaacgtgaag
    36721 cttttgcgag ttttccagat gatctcttgg ttcaatgttt tactaaggaa gggttggaaa
    36781 ctctagattt tactatagaa ctatccttga cctgtgattt ggcttctgat ggaaagtatg
    36841 agcaggaaaa atctgattac aaggagtgta agttggatat tactgattct catatcttga
    36901 tgaagggaag agttaaggat aatgatctgc ggtttgctag ttatctagct tgggaaacgg
    36961 atggagatat tagagtttgg tcagataggg ttcagatatc aggagccagt tatgccaatc
    37021 tcttcttggc cgctaagacg gattttgccc aaaatcctgc tagcaattat cgcaagaaac
    37081 tagatttaga gcaacaggtg atagacttgg tggacacagc taaagaaaag ggctataccc
    37141 aattgaaatc aaggcatatc gaggactacc aagccttatt ccagcgtgtt caattggatt
    37201 tggaagctga tgttgacgca tccactacag atgatttgtt aaaaaattat aagccacaag
    37261 aagggcaggc tttggaggag ctgttcttcc agtatggacg gtatttattg attagttcgt
    37321 ccagagactg cccagatgct ctaccagcta acctacaggg agtctggaat tcggactatc
    37381 acttaaatgt caatctgcag atgaattatt ggccagccta tgttaccaat ctcctagagg
    37441 cggtctttcc ggtcatcaac tatgtagatg atttgcgtgt ctatggtcgt ctagcggctg
    37501 taaagtatgc aggaatcgtc tctcaaaaag gtgaggaaaa tggttggttg gttcatactc
    37561 aagcgactcc ctttggttgg acggcacctg gttgggatta ctattggggt tggtcaccag
    37621 ctgccaatgc gtggatgatg caaaccgttt atgaagccta ttcattttat agggaccaag
    37681 actatctcag ggagaaaatt tatcccatgt tgagggaaac ggttcgtttt tggaatgcct
    37741 ttttacataa ggatcagcag gcgcagcgtt gggtgtcttc tccgtcttat tctccagaac
    37801 atgggccgat ttcgattggc aatacctatg accaatctct gatttggcag ttatttcatg
    37861 attttattca ggctgctcag gaattgggac tggatgagga cttgttgact gaggttaagg
    37921 agaagtctga tttactaaat cctttgcaaa tcactcaatc tggtcgaatc agggagtggt
    37981 atgaggagga agagcagtat tttcaaaatg agaaagtgga ggcccagcat cggcacgctt
    38041 cccatctagt gggactctat cctggcaatc tctttagcta caagggacaa gagtatattg
    38101 aagcggcgcg tgctagcctc aatgatcgtg gagatggcgg cacaggctgg tccgaggcta
    38161 ataagatcaa tctctgggcg cgtttgggag atggcaatcg agcccataaa ttattggcag
    38221 agcagttaaa gacatccacc ttgcaaaatc tttggtgtag ccatcctcct tttcagatag
    38281 atggtaattt tggtgctact agtggcatgg cagaaatgtt actccagtct catgcagctt
    38341 atctggtacc tctagctgcc ctacctgatg cttggtcaac aggttctgtt tcaggcttaa
    38401 tggcacgtgg acattttgaa gtgagcatga gctgggaaga taaaaaactc ttacagttga
    38461 ccattttatc aaggagtgga ggagatttgc gagtttctta tccagatatt gagaagagtg
    38521 tgattaaaat gaatcaagaa aaaataaaag cgaaatgcat ggggaaagat tgtatttcgg
    38581 tggcaacagc agaaggtgat cttgttcaat tttattttta agaagatgtt ataaggcagt
    38641 aatttgaaac tgccttttaa taaggattta agaatataag cagttttcaa ctagttgaaa
    38701 aaacgttata atgataatag gaagtaatac tcaatgaaaa tcaaagagca caaactagga
    38761 agctagccgc aggttgctca aaacagtgtt ttgaggttgc agatggaagc tgacgtggtt
    38821 tgaagagaga ttttcgagga gtataatttg tttgatagag ggtgggtctg atggcttata
    38881 ttgagatgaa acactgttac aagcgttatc aggttgggga cacggagatt gtggccaatc
    38941 gtgatgtgaa ttttgagatt gaaaaggggg agctggttat tatccttggt gcttcaggtg
    39001 caggcaagtc aacagttctt aaccttcttg ggggaatgga taccaatgat gaaggggaaa
    39061 tctggattga tggtgttaat attgcggatt atagttccca ccagcgcacc aattaccgta
    39121 gaaatgatgt ggggtttgtt tttcagtttt ataatctagt ttctaatctg acagctaagg
    39181 aaaatgtgga actggcttct gaaattgtga cagatgcctt gaattctgat caggtcttga
    39241 cagatgtagg tctggctcat cgtctcaata actttccagc ccagctttct ggaggggagc
    39301 aacagcgagt ctccattgca cgcgcggtag ccaaaaatcc taaaattctt ctttgtgatg
    39361 aacctactgg agccttggat tatcagacgg gcaagcaggt tttgaaaatt ctccaagaca
    39421 tgtctcgtca aaagggagcg acggtgatca tcgtgactca taatggagct ttggcgccca
    39481 ttgctgatcg cgtgattcat atgcacgatg ccagtgtcaa ggatgtggtg ctcaaccagc
    39541 atcctcagga tattgacagt ttggagtact agcatgatca agcgaaaaac ttattggaag
    39601 gacttaattc agtccttcac aggctccaag gggcgttttt tatccatctt gatcctgatg
    39661 atgttgggat ctctagcctt agtaggcctc aaagtaacca gtcccaacat ggaggcgaca
    39721 gctaatgctt atttaacaac tgctcaaacc ttggatttgg cagtcatgtc taactatggc
    39781 ttggatcaag cagaccaaga agaactaaaa cagacggagg gcgcagaggt cgagtttggc
    39841 tatttgacag atgtgactat ggataatggg caggatgcca ttcgactgta ctccaaacca
    39901 gagcgaattt caacctttca gctaagaaag ggacgacttc ctcagtcaga caaggaaatc
    39961 gctttggcca ctcatttgca aggccaatac agcgtgggac aggagattag ttttaaagaa
    40021 aaagaagagg gtcattcctc tttaaaagac catacttata ccattactgg ttttgtggat
    40081 tcggctgaaa tcctctccca gcgagatatg ggctacgcag gaagtggaag tgggactctg
    40141 acagcctatg gggtgatttt acctagtcag tttgatcaga aagtctacaa tatagctcgt
    40201 ttgaaatatc aagatttagc aggtttaaat gccttttcat cagcttatga agaaaaatcc
    40261 aagcaacatc aggaagagct tgaacaaatt ttatcagata atggcaaggt acgtctgcaa
    40321 cttttgaaaa aagaaggaca agagtctcta gacaaggggc aagagaccct tgacaaggct
    40381 cagactaatt tgcaggaagg caagcgtcgt ttagcagctg ctcaagctcg tatacaggct
    40441 caagaaagtc aactagcctt gtttcctcaa gttcagagag agcaggctag tgctcaactt
    40501 acccaagcca agcaggaatt gggcaaggaa gaggccaaac taaagcaagc tgaacaaaat
    40561 ctagcccaag aaaaggaaaa attagaaaaa catcagcaag tcttggacga tttggcggag
    40621 ccaaggtatc aggtctataa tcgtcagacc atgccaggtg gtcagggcta tcttatgtat
    40681 agcaatgctt catccagtat tcgagcagtg ggcaatatct ttcctgtggt actttatgcc
    40741 gtagcagcca tggtgacctt tacgaccatg actcgctttg tagacgaaga gcgaactcat
    40801 gcagggattt ttaaggcctt gggttaccgt agtaaggata ttatcaccaa gtttctcctt
    40861 tatggactag tagctgggac tgtcggaacg gctctaggta gtatacttgg tcattatttg
    40921 ctagccagtg taatttcaag tgtcattaca aaaggcatgg tggtgggaga aacccagatt
    40981 cagttctatt ggacctatag cttactagct cttgtcttga gcttgttggc gagtgtgtta
    41041 ccagcctatc tggtggcttg gagggaactt catgccgaag cagcccagct tctacttcct
    41101 aaacctcctg tcaaaggagc taaaatctta ttggagcgta tcggttttat ctggcgtcgt
    41161 ctcagtttta ctcataaggt aacagcccgc aacatctttc gttataagca gagaatgttg
    41221 atgacaatct ttggtgtggc aggttctgta gctctgctct ttgcaggttt gggaatccaa
    41281 tcttctgtag caggagttcc gtctaaacag tttcaacaaa tccaacagta tcagatgctt
    41341 gtctctgaaa atcctagtgc gaccaatcag gacaaggtag agctagcaga agtgttgaaa
    41401 gggcaggaga tactagccta ccagaaaatc tattctaaaa cgctagacaa ggatttcaaa
    41461 ggcaaggctg gtcttcaaaa cattactctt atgatgatag agaaggaaga tttgactccc
    41521 tttatccatc ttcaacatca tcagcaggag ctgacattaa aagatggcat cgttattaca
    41581 gctaaactcg cccagctggc aggtgtcaag gttgggcaga ctttagaaat tgaaggtaag
    41641 gaactaaagg tcgctgctat tactgagaac tacgttggtc actttattta tatgagtcag
    41701 gctagctatg agcaacttta cggacagcta ccccaagcca acacttatct ggtctcatta
    41761 agggatacca gtgcaactag tatcgaaagt caggcgggct tgcttatgaa tcaatctgcg
    41821 gtgtccagcg ttgtccaaaa tgcttcagcc attcgactct tcgactctat cgctagctca
    41881 ctcaatcaga ccatgaccat cttggtcatc gtatcggttc tattagctat tgtcatcctt
    41941 tacaatctga ccaatatcaa cgtagctgag agaatccgtg aactctccac tatcaaggtt
    42001 ctcggttttc ataataatga agtcaccctc tacatttacc gtgagacgat tgtgctgtcc
    42061 cttgtgggaa tcgtacttgg tctggtagct ggtttctatt tacaccaatt tttgattcaa
    42121 atgatttcgc ctgcgactat tctcttttat ccgcaggtag gctgggaagt ctatgtaatc
    42181 ccagtggcag cagtaagcat cattttgacc ttgcttggtt tcttcgtcaa ttattatctg
    42241 agaaaggttg acatgttaga agccctgaaa tctgtagagt aaggtagtta tttttagctg
    42301 attgaacttc tatttactaa tattcaaaaa tcctccgttt caaagagcag ggaactcttt
    42361 gtgacagagg attttttcta tagggcttta gcagctgcaa ttgcggcttc gaagtttggc
    42421 tcagaattga tattatccac gtattcaacg tagcgaatcg tattgtcagt atcgaggaca
    42481 aagactgcgc gtgctaatag gtgccattcg ttgatcaaga gggcataatc gtgcccgaaa
    42541 gaatggtcaa agtagtctga aagcataatg gcattgtcaa ggccttcagc accgcaccaa
    42601 cgtttttgag caaaaggtag gtccattgaa acagtcaata cgaccgtgtt gtccagtcca
    42661 gccaattctt cattaaaacg acgtgtttga gttgagcaga tgcctgtatc gatagaagga
    42721 acgacactca agactttttt cttgccatca aaatcagcca gagatttttt agaaagatct
    42781 gttgtagtaa gagaaaaatc aagcgccttg tcgccgactt gtagttgttt acctgtaaag
    42841 ctcacaggat ttccgagaaa agttaccata ggatactcca atcttttttc ttccattgta
    42901 tctgaaacag tcagaatttt ccaatgattt gaccggaaat gtgggcatag aaaaaacgcc
    42961 agctcatatg agaatgacgt ttttcagagg cttattttgc caatccttca gcaatcttgt
    43021 caaggttgta tttcatcatg ctgtagtagc tgtcgccttc tttaccttgt tctgcgatag
    43081 agtcagtaaa gatttgtgcg tagattggga tgtttgtgtc ttgagaaacg gttttcattg
    43141 gacggtcatc cacacttgat tctacaaaga gtgatggaac ttttgtttgg cgaagttttt
    43201 caaccaaggt cttgatttgt tcaggagttc cttcttcttc agtattgatt tcccagatgt
    43261 aggcacttgg aacaccatag gctttagaga agtatttgaa tgctccttcg ctggttacaa
    43321 tgagtttctt ttcagcaggg atcttattaa atttatcctt actttcttta tcaagtttgt
    43381 ctaacttatc agtatattct ttgagatttt tttcatagaa ttctttattg ttagggtctt
    43441 tggcgctcaa ttgtttggcg atatttttag caaaaataat accgttttca aggttaagcc
    43501 aagcgtgtgg gtcttctttt cctttttcat tttgaccttc aaggtagata acatcaacgc
    43561 cgtcgctgac tgcgaagtag tctttgtttt cagttttctt ggcattttct accaattttg
    43621 taaaccaagc attgccacct gtttcaaggt tgataccgtt atagaaaatc aaatcagcct
    43681 cagaagtttt cttaacgtct tcaggaagtg gttcgtattc gtgtgggtct tgcccaatcg
    43741 gaacgatact atgaaggtca attttgtcac cagcaatatt tttagtaata tcagcgatga
    43801 ttgagtttgt agcaacaact tttagttttt gaccagaagt tgtatctttt tttccgctag
    43861 cacatgctac aagaatgatt gcagaaagaa agagaacgag taatgtacct aattttttca
    43921 ttagatcctc caatttatta gggctttgcc ccttatttta acaaatgttt atttttcagt
    43981 ttcaaatatc gttgtttggg agcgataaag aagctaatga gaaagaaact agcagctgta
    44041 agcacgatac tagaacctgc cgcaacatta aaactatagc caataaagag tcccaaaact
    44101 gaagcagtag ctccgaaggt tgaggaaagg aaaatcatgc ttttcagact attagcatac
    44161 agataagcag ttgcagctgg ggtaatcagc atggctacaa tcaggatagt tccgacactt
    44221 tgcatggctg tcacagacac gagagtcagg agtaccatga gaaggtagtg atagaaattg
    44281 acaggcattc ccatggcttt agccaagagt tcatcaaagg aagttatcaa gagttgcttg
    44341 aagaaaatcc agattaacaa gaggatggct gcccccacac ccatagtaat aaacatatcc
    44401 gtatcttgga cggccaggat attaccaaaa aggatatgga aaaggtcagt tgaactttta
    44461 gcgacaccaa tcaagatgat accgagggct aagaaagaag aaaaggtaat gccgatggcg
    44521 gtatcgcttt tgataatcga gtttcctttg atgtaggtaa tgatgatggc agctagcaat
    44581 ccaaagacaa tggctccgat aaagaagtca aggcccaaga tgaaggagag ggctacacct
    44641 ggtaagacag catgtgaaat ggcatctccc atgagtgaca tcccacgtag aataatgaaa
    44701 catcccacgg ctccagctac aatcccgacg acaatagctg ttatcaaggc attttgtagg
    44761 aaatggaatt tttgcaatcc atcgataaat tctgcaatca taggtcacct ccattgaaaa
    44821 agagttgatt accgtaagct tcttttagat tggtttcggt aaaagtttct tttgttggac
    44881 caaaggcaat cacttctcga ttgacaagta agacttgatc gaagtagtgg ggaatcttgc
    44941 tgaggtcgtg gtgaacgatg agaaccgtct tcccagcttt tttcaaatct ctcagcgtat
    45001 tcatgatgat ttcctcactg acagagtcaa tcccagcaaa gggttcatcc aagaggatat
    45061 agtcggcttc ctgcaccaaa catctggcaa tcaagacccg ctggaattga cctccagaca
    45121 gttgactaat ttgacgttca gcgtagtcag ctaggccgac gatttcaagg gcctcttgca
    45181 ctttcttcca atgtttagcc tttaaacttc gaaagagagg aatagaggga aatagtccta
    45241 acgagacgca ttccttgacc ttgatgggaa agttgtagtc gatattgatt ttttgttcga
    45301 cataggcaat tcggtgtaag gattttttaa cttccttgtc atcgagaaat gcctgacctt
    45361 gatgtgggat aattcccagc atacctttta atagtgttga tttcccagcg ccgtttggac
    45421 caatgatgcc ggtaattgtt ggtccatgga gcactagtga aatatcctta agtgccaacg
    45481 tttctttgta ggagacactg aggttttcga tacgtatcat aaacttgtat
//
LOCUS       DAVKLW010000009        45429 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKLW010000009 DAVKLW010000000
VERSION     DAVKLW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.009"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(156..614)
                     /locus_tag="VW324_000491"
     CDS             complement(156..614)
                     /locus_tag="VW324_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9726170.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(611..1051)
                     /locus_tag="VW324_000492"
     CDS             complement(611..1051)
                     /locus_tag="VW324_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9726171.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1407..3356
                     /gene="mutL"
                     /locus_tag="VW324_000493"
     CDS             1407..3356
                     /gene="mutL"
                     /locus_tag="VW324_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9726172.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3681..4148)
                     /locus_tag="VW324_000494"
     CDS             complement(3681..4148)
                     /locus_tag="VW324_000494"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9726173.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4149..5354)
                     /locus_tag="VW324_000495"
     CDS             complement(4149..5354)
                     /locus_tag="VW324_000495"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9726174.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5374..6009)
                     /gene="ribE"
                     /locus_tag="VW324_000496"
     CDS             complement(5374..6009)
                     /gene="ribE"
                     /locus_tag="VW324_000496"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9726175.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5994..7094)
                     /gene="ribD"
                     /locus_tag="VW324_000497"
     CDS             complement(5994..7094)
                     /gene="ribD"
                     /locus_tag="VW324_000497"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9726176.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7229..7343)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7500..8093
                     /gene="ruvA"
                     /locus_tag="VW324_000498"
     CDS             7500..8093
                     /gene="ruvA"
                     /locus_tag="VW324_000498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9726177.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8103..8666
                     /locus_tag="VW324_000499"
     CDS             8103..8666
                     /locus_tag="VW324_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9726178.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8663..9340
                     /locus_tag="VW324_000500"
     CDS             8663..9340
                     /locus_tag="VW324_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9726179.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9495..10526)
                     /locus_tag="VW324_000501"
     CDS             complement(9495..10526)
                     /locus_tag="VW324_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9726180.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLSHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10781..11464)
                     /locus_tag="VW324_000502"
     CDS             complement(10781..11464)
                     /locus_tag="VW324_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9726181.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGSDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11476..12420)
                     /locus_tag="VW324_000503"
     CDS             complement(11476..12420)
                     /locus_tag="VW324_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9726182.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12552..15383
                     /gene="uvrA"
                     /locus_tag="VW324_000504"
     CDS             12552..15383
                     /gene="uvrA"
                     /locus_tag="VW324_000504"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9726183.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15376..16437
                     /locus_tag="VW324_000505"
     CDS             15376..16437
                     /locus_tag="VW324_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9726184.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16569..16967
                     /gene="spx"
                     /locus_tag="VW324_000506"
     CDS             16569..16967
                     /gene="spx"
                     /locus_tag="VW324_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9726185.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            17033..17602
                     /locus_tag="VW324_000507"
     CDS             17033..17602
                     /locus_tag="VW324_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9726186.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17689..17955
                     /locus_tag="VW324_000508"
     CDS             17689..17955
                     /locus_tag="VW324_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9726187.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17959..18378
                     /gene="ruvX"
                     /locus_tag="VW324_000509"
     CDS             17959..18378
                     /gene="ruvX"
                     /locus_tag="VW324_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9726188.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18394..18699
                     /locus_tag="VW324_000510"
     CDS             18394..18699
                     /locus_tag="VW324_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9726189.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18957..20207
                     /locus_tag="VW324_000511"
     CDS             18957..20207
                     /locus_tag="VW324_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9726190.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20204..20746
                     /locus_tag="VW324_000512"
     CDS             20204..20746
                     /locus_tag="VW324_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9726191.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            20892..22424
                     /gene="cls"
                     /locus_tag="VW324_000513"
     CDS             20892..22424
                     /gene="cls"
                     /locus_tag="VW324_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9726192.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22682..24220
                     /locus_tag="VW324_000514"
     CDS             22682..24220
                     /locus_tag="VW324_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9726193.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24340..26547
                     /gene="nrdD"
                     /locus_tag="VW324_000515"
     CDS             24340..26547
                     /gene="nrdD"
                     /locus_tag="VW324_000515"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9726194.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26559..26699
                     /locus_tag="VW324_000516"
     CDS             26559..26699
                     /locus_tag="VW324_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726195.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26743..27249
                     /locus_tag="VW324_000517"
     CDS             26743..27249
                     /locus_tag="VW324_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726196.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            27242..27832
                     /gene="nrdG"
                     /locus_tag="VW324_000518"
     CDS             27242..27832
                     /gene="nrdG"
                     /locus_tag="VW324_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9726197.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27829..28446
                     /locus_tag="VW324_000519"
     CDS             27829..28446
                     /locus_tag="VW324_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9726198.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28712..29020
                     /gene="rpsJ"
                     /locus_tag="VW324_000520"
     CDS             28712..29020
                     /gene="rpsJ"
                     /locus_tag="VW324_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9726199.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29237..29863
                     /gene="rplC"
                     /locus_tag="VW324_000521"
     CDS             29237..29863
                     /gene="rplC"
                     /locus_tag="VW324_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9726200.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29888..30511
                     /gene="rplD"
                     /locus_tag="VW324_000522"
     CDS             29888..30511
                     /gene="rplD"
                     /locus_tag="VW324_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9726201.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30511..30807
                     /locus_tag="VW324_000523"
     CDS             30511..30807
                     /locus_tag="VW324_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9726202.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30825..31658
                     /gene="rplB"
                     /locus_tag="VW324_000524"
     CDS             30825..31658
                     /gene="rplB"
                     /locus_tag="VW324_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9726203.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31762..32043
                     /gene="rpsS"
                     /locus_tag="VW324_000525"
     CDS             31762..32043
                     /gene="rpsS"
                     /locus_tag="VW324_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9726204.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32055..32399
                     /gene="rplV"
                     /locus_tag="VW324_000526"
     CDS             32055..32399
                     /gene="rplV"
                     /locus_tag="VW324_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9726205.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32412..33065
                     /gene="rpsC"
                     /locus_tag="VW324_000527"
     CDS             32412..33065
                     /gene="rpsC"
                     /locus_tag="VW324_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9726206.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33069..33482
                     /gene="rplP"
                     /locus_tag="VW324_000528"
     CDS             33069..33482
                     /gene="rplP"
                     /locus_tag="VW324_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9726207.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33492..33698
                     /locus_tag="VW324_000529"
     CDS             33492..33698
                     /locus_tag="VW324_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9726208.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33723..33983
                     /gene="rpsQ"
                     /locus_tag="VW324_000530"
     CDS             33723..33983
                     /gene="rpsQ"
                     /locus_tag="VW324_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9726209.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            34009..34377
                     /gene="rplN"
                     /locus_tag="VW324_000531"
     CDS             34009..34377
                     /gene="rplN"
                     /locus_tag="VW324_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9726210.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34455..34760
                     /gene="rplX"
                     /locus_tag="VW324_000532"
     CDS             34455..34760
                     /gene="rplX"
                     /locus_tag="VW324_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9726211.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34784..35326
                     /gene="rplE"
                     /locus_tag="VW324_000533"
     CDS             34784..35326
                     /gene="rplE"
                     /locus_tag="VW324_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9726212.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35344..35613
                     /gene="rpsN"
                     /locus_tag="VW324_000534"
     CDS             35344..35613
                     /gene="rpsN"
                     /locus_tag="VW324_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9726213.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35898..36296
                     /gene="rpsH"
                     /locus_tag="VW324_000535"
     CDS             35898..36296
                     /gene="rpsH"
                     /locus_tag="VW324_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9726214.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36488..37024
                     /gene="rplF"
                     /locus_tag="VW324_000536"
     CDS             36488..37024
                     /gene="rplF"
                     /locus_tag="VW324_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9726215.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37108..37464
                     /gene="rplR"
                     /locus_tag="VW324_000537"
     CDS             37108..37464
                     /gene="rplR"
                     /locus_tag="VW324_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9726216.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37482..37976
                     /gene="rpsE"
                     /locus_tag="VW324_000538"
     CDS             37482..37976
                     /gene="rpsE"
                     /locus_tag="VW324_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9726217.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37948..38172
                     /gene="rpmD"
                     /locus_tag="VW324_000539"
     CDS             37948..38172
                     /gene="rpmD"
                     /locus_tag="VW324_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9726218.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38317..38757
                     /gene="rplO"
                     /locus_tag="VW324_000540"
     CDS             38317..38757
                     /gene="rplO"
                     /locus_tag="VW324_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9726219.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38770..40080
                     /gene="secY"
                     /locus_tag="VW324_000541"
     CDS             38770..40080
                     /gene="secY"
                     /locus_tag="VW324_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9726220.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40231..40869
                     /locus_tag="VW324_000542"
     CDS             40231..40869
                     /locus_tag="VW324_000542"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9726221.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40986..41204
                     /gene="infA"
                     /locus_tag="VW324_000543"
     CDS             40986..41204
                     /gene="infA"
                     /locus_tag="VW324_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9726222.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41229..41345
                     /gene="rpmJ"
                     /locus_tag="VW324_000544"
     CDS             41229..41345
                     /gene="rpmJ"
                     /locus_tag="VW324_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9726223.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41363..41728
                     /gene="rpsM"
                     /locus_tag="VW324_000545"
     CDS             41363..41728
                     /gene="rpsM"
                     /locus_tag="VW324_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9726224.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41746..42129
                     /gene="rpsK"
                     /locus_tag="VW324_000546"
     CDS             41746..42129
                     /gene="rpsK"
                     /locus_tag="VW324_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9726225.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42172..43107
                     /locus_tag="VW324_000547"
     CDS             42172..43107
                     /locus_tag="VW324_000547"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9726226.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43119..43505
                     /gene="rplQ"
                     /locus_tag="VW324_000548"
     CDS             43119..43505
                     /gene="rplQ"
                     /locus_tag="VW324_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9726227.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43770..44036
                     /locus_tag="VW324_000549"
     CDS             43770..44036
                     /locus_tag="VW324_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9726228.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            44046..45383
                     /locus_tag="VW324_000550"
     CDS             44046..45383
                     /locus_tag="VW324_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9726229.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        13673 a         8629 c         9976 g        13151 t
ORIGIN      
        1 gaatagtaca cctctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcgat
       61 ttatcctgtt cttatttcat ttcactatag taaggtgcag caggataaag aaaaaccgag
      121 aagtttcttt ctcggatttg cttattatga ggttcctatt ttctatgctc caagagttga
      181 ttgatgtgat ctactttttt agcctctctt ttatagagaa tagcccagat gatgagatag
      241 acaatcgcaa actcgataat gagctggagg tagaaaatcc agtggaaagg gaaccaacca
      301 gcaagagttg ctagtgggac aaagccagcc agcataagga agaaatgagt cagagtggca
      361 cgaagcatgc tccagtcacg gctaaataag cggttgccaa agttgaagag cataccgatg
      421 gttgcccaga taagtgtgca gtagagcaag accagggcac cgtgaacctg atgctgatcc
      481 atcacttggc ctatgagaga gtagggattt agtggtgcgt aggtatttgg tgcataaatg
      541 agtgaaaaga ggatagagag gatgaggccg ataagaacac cggtagctgc atcgtgaaat
      601 acttgttttt tcatagttct aatttctcct tgatggtttt tagataacgg cgtgaagagt
      661 aggtgaagct ttcgtttttc aagaaaattt ctaccagacc gtttggcgtg agcttgaggt
      721 gagagatgga atcgatattg atgatttctg attgggaaat ttggataaaa ttgcttggca
      781 agagtttaag aacctgatag agtcgcaaat caatgctgta ggtctgactc gcggtttctg
      841 ctagaacctt ccgattctcg atatagaagc gctgaatctt gccaatctca actagataga
      901 cctgatcatc gatttttcct ttgatttttt ctctttggtc cagattttct gcgaactcga
      961 tgactttctg gactttttcg gtttcttgag gtgcttggac aatcagcttt tcctcctcgt
     1021 aagtctcact aatctgtagt tctactttca taaatttctc tccttgtttt tcatacaaaa
     1081 ttaatctcac ttcttgtaca cctttattaa tactcttcaa aaatctcttc aaaccacgtc
     1141 agcgtcgtct tgccgtagga tagttgttga ctttgtcagt tttatctaca acctcaaaac
     1201 agtgttttga gctgtctgcg tctagctttc tagtttgctc tttgattttt attgagtata
     1261 ccactatttt actccctctg gcaagggact ttgtctatgt ggagggattg ggctcctatg
     1321 tggtggagtt tttctgttct ttctgaaata tggtataata gcactaatta atttctagga
     1381 aaatagatac agaaaggggc tgaaagatgt ctcatattat tgaattgcca gagatgctgg
     1441 caaaccaaat cgcagctggg gaggtcattg aacgtcctgc cagtgtggtt aaagagttgg
     1501 tagaaaatgc cattgacgcg ggctctagtc agattatcat tgagattgag gaagctggtc
     1561 tcaagaaggt tcaaatcacg gataacggtc atggaattgc ccacgatgag gtggagttgg
     1621 ccctgcgtcg ccatgcgacc agtaagataa aaaatcaagc agatctcttt cggattcgga
     1681 cgcttggttt tcgtggtgaa gccttgcctt ctattgcgtc tgttagtgtc ttgactctgt
     1741 taacggcggt ggatggtgct agtcatggaa ccaagttagt cgcgcgtggg ggtgaagttg
     1801 aggaagtcat cccagcgact agtcctgtgg gaaccaaggt ttgtgtggag gatctctttt
     1861 tcaacacgcc tgcccgtctc aagtatatga agagccagca agcggagttg tctcatatca
     1921 ttgatattgt caaccgtctg ggcttggccc atcctgagat ttcttttagc ttgattagtg
     1981 atggcaagga aatgacgcgg acagcaggga ctggtcaatt gcgccaagca atcgcaggga
     2041 tttacggttt ggtcagtgcc aagaagatga ttgaaattga gaactctgac ctagatttcg
     2101 aaatttcagg ttttgtgtct ttacctgagt tgactcgggc taaccgcaat tatatcagcc
     2161 tcttcatcaa tggccgttat atcaagaact tcctgctcaa tcgtgctatt ttggatggtt
     2221 tcggaagcaa gcttatggtt ggacgttttc cactggctgt cattcacatc catatcgacc
     2281 cttatctagc ggatgtcaat gtgcatccaa ctaagcaaga ggtgcggatt tccaaggaaa
     2341 aagaactgat gaccctggtt tcagaagcta ttgcaaatag tctcaaggaa caaaccttga
     2401 ttccagatgc cttggaaaat cttgccaaat cgaccgtgcg caatcgtgag aaggtggagc
     2461 aaactattct cccactcaaa gaaaatacgc tctactatga gaaaactgag ccgtcaagac
     2521 ctagtcaaac tgaagtagct gattatcagg tagaattgac tgatgaaggg caggatttga
     2581 ccctgtttgc caaggaaacc ttggaccgat tgaccaagcc agcaaaactg cattttgcag
     2641 agagaaagcc tgctaactac gaccagctag accatccaga gttagatctt gctagcatcg
     2701 ataaggctta tgacaaactg gagcgagaag aagcatccag cttcccagag ttggagtttt
     2761 tcggacaaat gcacgggact tatctctttg cccaagggcg agatggactt tacatcatag
     2821 atcagcacgc tgctcaggaa cgggtcaagt acgaggagta ccgtgaaagc attggcaatg
     2881 ttgaccagag tcagcagcaa ctcctagtgc cctatatctt tgaatttcct gcggatgatg
     2941 ccctgcgtct caaggaaaga atgcctctct tagaggaagt gggcgtcttt ctagcagagt
     3001 acggagaaaa tcaatttatt ctacgtgaac atcctatttg gatggcagaa gaagagattg
     3061 aatcaggcat ctatgagatg tgcgacatgc tccttttgac caaggaagtt tctatcaaga
     3121 aataccgagc agagctggct atcatgatgt cttgcaagcg atctatcaag gccaatcatc
     3181 gtattgatga tcattcagct agacaactcc tctatcagct ttctcaatgt gacaatccct
     3241 ataactgtcc tcacggacgt cctgttttgg tgcattttac caagtcggat atggaaaaga
     3301 tgttccgacg tattcaggaa aatcacacca gtctccgtga gttggggaaa tattaaaagt
     3361 ataaaaaagt ctgggaaaaa ttttcaaaat cagaaaaacg cataaaatca ggtgttcaaa
     3421 aaccttgatt ttatgcgttt tatcatggaa atagttactt catgttttcc taattctttt
     3481 cgaaactctt tttaaaccac gtcagtttta tcagtaatct caaaacagtg ttttgagcta
     3541 attttgccag ttttgtctgt aacatcgaag ttgtgtttta ccactctgcg actggtttcc
     3601 tagtttgctc tatgattttc acagagtatt aaattgcgat tttgccaagt ttctttattc
     3661 gtctaaaagt agagtctgtt ctatgcgtct aatgtacgaa ttaggttgac catttcaata
     3721 gctccttgtg cacactcaga acccttattt cctgctttgg taccagctcg ttctatggct
     3781 tgttcaattg tatctgtcgt tagcacacca aacataacag gaatttcgct atttaaactg
     3841 atttgggcga ttcccttaga tacctcgcta catacataat cataatgact tgtattccct
     3901 ctaatgacag ctcccaagca gataattgca tcatattttt tactttttgc catttttgat
     3961 gcaatcagtg gtatttcaaa agctcctgga acccaggcta cctctatatc tttctcgttt
     4021 acattctctc ttttgagatt atctagtgct ccagataata attttgaagt tataaattca
     4081 ttaaatctcg ctacaacaat acctatttta atattgtttg ctactaaatt accttcataa
     4141 gtgttcattt atttttcctc catatttaaa atgtgaccca ttcgattttt ctttgtttct
     4201 aaataaaaac tatcgtaagg attggcttct atttcgattg atattctact ggaaatggta
     4261 attccatatt tttctaactg ttcaaccttg tcaggattat ttgtcagtaa atgaagtgac
     4321 tgaagtccca gatctttaag catttgtgct ccaatatgat attctcttaa atcaccttca
     4381 aagcctaatg caagattggc atcaagcgta tccatgcctt gatcttgtaa atgataggct
     4441 tttaatttat tgataagtcc aattcctcgt ccctcctgtc gcaagtaaag taagacaccc
     4501 gaaccattct caacaatcat tttcatagct ttatcgaatt gctgtccaca atcgcaacgt
     4561 aaagagccta aaacatctcc tgttaaacat tcggagtgga cccgacataa tacattggct
     4621 tcatcctcta tgtttcccat aataagagca agatgatgtt ccccatttag tttatctata
     4681 tagctaattg ctttgaaatt accgtatcta gtaggcatat tgacagttga aactcgttct
     4741 atcagctgat catatacttt tctatattct tgtaattctt tgatggtaat tagtggaatg
     4801 ttgtgtttct tcgagaactg aattaaatca tctgttctca tcattttgcc atcatgattc
     4861 attatttcac aacataggcc acactctttt agtccagcta attttaataa atcaacagtt
     4921 gcttctgtgt gtccatttct ttctagaaca ccaccttttt ttgcaattaa aggaaacatg
     4981 tgtcctggcc tgcgaaaatc agagggtgtt atatcttcag ctacacacat acgtgcggtc
     5041 agtcctcttt cctcggcaga aatacctgtg gtcgtttctt tataatcaat tgaaactgta
     5101 aaagcagtct tatgattatc tgtattgttt tcaaccatag gtgaaagcat taattgatta
     5161 gctaaacttt cgctcatagg catacaaatt aatcctttgg cataagtagc cataaaatta
     5221 acattttctg ttgtagctgc ttgtgcagaa caaattaagt ctccttcatt ttctctatcc
     5281 ttgtcgtcta taacaagaac aagtcgtccc ttctgcaatg cttctaatgt ttcttgtatt
     5341 tttcgatatt ccattgactg attatccttt ctgctaaaat ccattttgat ataatagttc
     5401 cttagatatt tctgattttg gagagttatc catcagtttt tgcacatatt tacctaagat
     5461 atcattttca agatttactg tactcccgac ttgtttactt ttaagaatgg tttgtttcaa
     5521 ggtatgaggg ataacagata ctgaaaagtt tactttggag actttagcga cagtcagact
     5581 aatgccgtca attgtaatag atcctttttc aactattaaa tctaaaattt ctttttgtgt
     5641 gttgatttga taccatacag cattatcatc tttttttatt gacgagattt ttcctgtacc
     5701 atcaatgtgt cctgtaacga cgtgaccccc aagtcgaccg ttgatagata aggctctttc
     5761 tagattcacc tcacttccat gttttaatag agtaagagct gttcgactcc atgtttcatt
     5821 cattacatca actgtaaagg attgatgatt gaaatgagta actgtaagac agataccatt
     5881 tactgctata ctatcgccta aatggatatc cgttaatatt tttgaggctt taattgatag
     5941 tttacaatta cgagagtctt tctgtattct ttcaactttt ccgatttctt caattattcc
     6001 tgtgaacatg gataaatcac ttcactttct atgagatagt catttcctat ttgagaaaaa
     6061 gcataaggtt tcaatctaat agcgtcattt ggcaaagaaa tgccttcacc tccgacagga
     6121 aacttggcac tgcctccaaa aatttttggt gcaatatata ttttcagctc atcaacaatt
     6181 tgttgttcca aagcactcca attcattaga ctgccccctt ctagaactag gctatcaatc
     6241 tgcatgtttc ctagatgttg cattaaactc gataagtcta tatgattgcc ttttttcttt
     6301 atggaaagta tttcacagcc atgattttga tatagcttca ttttattttt gtcttcagag
     6361 gaagtggcaa tgtaagtttt aatatcattt gctgttttta cgattttaga ggtaagagga
     6421 gttcgtaaat gtgtatcgca tatgatacgg ataggatttt tcccttcctc caatctacat
     6481 gtcagcaaag gatcgtcttg aataacagta ttgactccca ccataattgc actaacatgg
     6541 tgtcgtaact gatgcacatg ctttcttgct tcttcttcag taatccattt ggattgattt
     6601 gttttagtgg ctatttttcc atccattgac attgcatatt tcataaaaac atagggtaca
     6661 tgctgggtaa tatactttct aaaacttttt attaagttaa gacactcatt ttctaaaatt
     6721 ccaacagtaa cttgaagatt attttcctca agtatcttta ctccttttcc agatacaata
     6781 ggattacagt ctaggcttcc aatgactact cttgtaatac cactatcgat tatagcatct
     6841 atacagggag gtgttttccc gaagtgacaa cagggttcaa gtgttacata aagcgtcgct
     6901 ccgacagggg attctctaca gtttttaaga gcatttctct cagcatgtgg gccaccaaaa
     6961 aactcatgat aaccttgtcc gatgatgtga ttatctttta caataactgc gccgaccata
     7021 ggattgggat tgacgtaacc agcccctttt tgtgccagtt ttattgctaa tttcatatat
     7081 tttgaatcgc tcatctcact acctccaaaa aaaatatacc ttgaataggg gactactcaa
     7141 ggcatacaaa agaaaactta tgcgattaac aaaaatactc tgaaatgaca agtaatcatt
     7201 tcagagcacg caaaaagcac aaatatactt ttatcttctt tcatccagac tatactgtcg
     7261 gctttggaat ttcaccaaat catgcctttc ggctcgtggg ctataccacc ggtagggaat
     7321 tacaccctgc cctgaagata gttattcaat tacagatgat tatagtactt aattttgaat
     7381 atgtcaacag ataaataccg attgtttttg atatactgta tttgtgataa tcgattctcg
     7441 ctcctcggat aaagaaaata tgatatacta gataaacgaa ataagagaga aggaatacta
     7501 tgtacgcata tttaaaagga atcattacca aaattactgc caaatacatt gttcttgaaa
     7561 ccaatggtat tggttatatc ctgcatgtgg ccaatcctta tgcctattca ggtcaggtta
     7621 atcaggagga tcagatttat gtgcatcagg ttgtgcgtga ggacgcccat ttgctttatg
     7681 gatttcgctc agaggatgag aaaaagctct ttcttagtct gatttcggtc tctgggattg
     7741 gtcctgtatc agctcttgct attatcgctg ctgatgacaa tgctggcttg gttcaagcca
     7801 ttgaaaccaa gaacatcacc tacttgacca agttccctaa aattggcaag aaaacagccc
     7861 agcagatggt gctggacttg gaaggcaagg tagtagttgc aggagatgac cttcctgcca
     7921 aggtcgcagt gcaagcaagt gctgaaaacc aagaattgga agaagctatg gaagccatgt
     7981 tggctctggg ctacaaggca acagagctca agaaaatcaa gaaattcttt gaaggaacga
     8041 cagatacagc tgagaactat atcaagtcgg cccttaaaat gttggtcaaa taggagcaga
     8101 gaatgacaaa acgttgttcg tgggtcaaga tgacaaaccc gctctacatc gcctatcatg
     8161 atgaggagtg gggtcagccc ctccatgatg accaagcatt gtttgagttg ttgtgtatgg
     8221 aaacctatca ggcaggcctg tcttgggaaa cggtactcaa caaacgccaa gctttccgag
     8281 aagcctttca tagctatcaa attcactcag tcgcagagat gactgagact gaattggaag
     8341 ccatgctgga gaatccagct atcattcgaa atagagccaa gctttttgct acacgcgcta
     8401 acgcccaagt ccttctacag ttacaggcag agtacggctc ttttgatgcc tatctttggt
     8461 cttttgttga ggggaaaatt gtcgttaacg atgttcctga ttatcgccaa gcgccagcta
     8521 aaacaccctt atctgagaaa ttagccaaag atctcaaaaa acgaggcttc aagttcacag
     8581 gcccagtcgc cgtattgtct tttctacagg ctgcagggct agttgatgac cacgagaatg
     8641 attgtgagtg gaaaggtctt aaatgatgtc taacaaaaat aagggaattc tgatttttgc
     8701 gattctctat acagtcctct ttgtgtttga tggcgttaaa ttgctggctt ctttaatgcc
     8761 atctgtcatt gcaaattatc ttgtttatgt agttttagct ttatatggct ccttcttgtt
     8821 caaggataga ttgatccaac aatggaagga gattagaaag actaaaagaa aattcttctt
     8881 tggcgtctta acaggatggc tctttctcat tctgatgact gttgtctttg aatttgtatc
     8941 agagatgttg aagcagtttg tgggactaga tggacaaggt ctaaatcagt ctaatattca
     9001 aagtaccttt caagaacaac cactactgat agctgttttt gcttgtgtca ttggacctct
     9061 ggtagaagaa ttatttttcc gtcaggtctt attgcattac ttgcaggaac ggttgccagg
     9121 tttactaagc attattctgg taggacttgt ttttgctctg actcatatgc acagtttggc
     9181 tctatcagag tggattggtg cagttggtta cttaggtgga ggccttgcct tttctattat
     9241 ttatgtgaaa gaaaaagaga atatctacta tcccctactt gttcacatgt taagcaacag
     9301 cctctcctta atcattttag ctatcagtat agtaaaatga aataagaaca ggacaaatcg
     9361 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
     9421 ttcaatttgc tgtaatatgt gatgaaaata ccagtaatga taccgagaaa aaagctgaga
     9481 aacttttccc agctttattt gttatagtca aagcgaatga cttgttcctg tgcatctaca
     9541 tgagcatgga ccccaaaggg tacaattgct cttggagttg cgtggccgac attcagatta
     9601 tagacaatcg ggatattgct gtcaatgata tccaatagtg cctctttata gtcgtcatgg
     9661 aaagtttcat ccataggttt tccgaccaag agtccattga tgaccgcgaa tatgccagtg
     9721 tcctttaaag ttagcaacat ctttttgaag tcttctggct taggcttttc ttcgcttgtt
     9781 tcgagcaaga ggattttccc ttcccagtct gacaagtcag ggaaaagttt gtatttttgg
     9841 cagagttccg tgctatctgc gtatcgagag ttgtcaaaga tatcgtagag ggattcgagg
     9901 caaccaccga ggatttttcc ctcgaactga gcacttcctt gcaacaagtc aaaacctgta
     9961 tttgtatgac tgacacgagg tgttcccagg gccgtgggac taaaatcagt tcgttcctca
    10021 taccaaacgt cactagggcg gatttctgaa attcttcccg tcttaatcaa ttctttaaag
    10081 tagtgaaggc tataggctag catttctttg tctaattcac aaatgtctgc taaaaaggat
    10141 tgaccataaa aagtcttgat tcctaattta tgcaacatga ggtggttcat ggttgtatcc
    10201 gagaagccaa gaaaaatttt ttgcttgata accttttgga gttggtcatt ttcaaaaaga
    10261 taaggtagca agcgataggt atcgtctcca ccgatggcac ataggatcat gtcgatgcta
    10321 tcatcagaaa aggcatgaat caaatcctct gcacgagctt caggatggtc cttgataaag
    10381 tctaatcctt ttaacgaatg ggacaaaaag atgggattga gtcccagatc cttgagacgt
    10441 tggataccca agtccacttc gtgtttgaca aaatcctctc cgataatgcc actagataaa
    10501 ctaacaatac caatagtaga aaccatatct catcctccta gaaatagatt gagcctattt
    10561 tatcacaaaa tataagagaa agctatgtgg gatttcagaa gaatactttt aatactcttc
    10621 gaaaatctct tcaaaccacg tcagttttat ctgcaacctc aaagctgtgc tttgagcaac
    10681 ctgcagctag cttcctagtt tgctctttga ttttcattga atataactca aaaaagtaag
    10741 tgaaaaagca accgcacctg cagttgcttt tatcgttttc ttattcttga tagcgtgttg
    10801 gtcctgcact tgcactacgg atattcaagc gtttcttgag atagaagcga agggctagga
    10861 gggctgctcc aataatagct agtggcaatg gatccagtac tgggttaagg ctagctggta
    10921 ggaagcttgt tgcaaagaag acaagcaacc aaaggaacat agaagctagg ataactagta
    10981 cagatttcca gaaaggtgga cgttgactgc gatccatatc tgatccatag tattggtaaa
    11041 caaagtagta catcaagtag aaggcaaatc caccaaccag tccaactaat agaagagtaa
    11101 tcaatccata gccgaaagct tggtctgccg caaagaaggt tgtgagggcg ctgacaaggg
    11161 caaagaggct agtgatgaaa agagctgagt ccataatcat cagttttggg tcatcatttt
    11221 cttttggatg ctctttttcg tactgctctt tgacagtgaa gctatgagcc caatgagttg
    11281 gtgcgccgta tagggaacgg gcagttgtac ctttagattg ctcctcaagg atttggggaa
    11341 tgacttcctc aaaaatagtc tggatttcag cgtctgtttt cccatctttg atgaattgtt
    11401 gggtagcaat gtggacaaac tcttggtttt tcttagttaa tttttgtaga tcaatttgag
    11461 acataggaac tcctcttaga accatttttt atggatgaga tagagagtga gcgagacact
    11521 catagcaaag gcgataaaga cgattaacca gaaggcattt ggctctccgt ttagggggat
    11581 ttcattatcc ttaaagttca tcccgtaggc agaaaagacc atggttggga tggacatgac
    11641 gatggtcaca agggccaagg ttttcatgat gttgttctgg ttgttagaaa tgatagaggc
    11701 aaaggtctct gtcatagaat gcaagacgtt tccataaata tctgccatct cgatggcctg
    11761 ttgggtttca atcagggtgt cttcaagcag gtcttcgtcc tcaaggtatt tcttgatatt
    11821 gctggttgaa ctggtcaatt tcttaatcac gcgctcattt gttttgaggg aggccttgaa
    11881 atagacgata gttttttcca attccatgag ctcaatcaat tcttcattac gagttgattg
    11941 atgcagttga ctttcgattt gttcactctt gcggtcgatt gaacgaaggg ctgttaggta
    12001 aagctctgca ttgcgataaa gaatttgaaa gataaaacgt gaacgcatga aggtatagaa
    12061 attacgcaat cgacggttga taaagacatc aaggacaggt agtggttcca aacacgtagt
    12121 gataatggtt tcctcagtga tgataatacc aagcgggatg gttacgtagt aggtgcggtt
    12181 atttctttcc tccgtgaccg gcacgtctac gataatcagg gtatactcgt cttcaatggt
    12241 aatacgagac atttcttccg catcgagcgg tgctcgaagg tcggcaatat caatatcgaa
    12301 ggtgttggcg atttcgagtg attcattttg agtcggattg acgagattga tccaagtacc
    12361 cggttcaagc gtatcgatct ctttaaattc agttgttgta gagagaaaaa cttgtttcat
    12421 atcccttatc ctttctcatt tttcagattt tttcacactg tactattata ctacaaaatc
    12481 ggccttttgg gtaatagaat ctcacttttt ttggtataat ggtaagcaat aatggactag
    12541 aaagaacaaa gatgcaagat aaaattgtca ttcatggggc gcgtgcccat aatttaaaaa
    12601 atattgatgt ggagattccg cgagacaagt tggttgtcgt gactggcttg tcaggttcag
    12661 ggaagtccag tctggctttt gatactctct atgcggaggg acaacgtcgc tatgtagaga
    12721 gtttgtctgc ctacgctcgt cagttcttgg gaaatatgga aaaaccagat gtagatgcca
    12781 ttgatggtct cagcccagct atttccatcg accagaaaac gactagtaaa aatcctcgct
    12841 cgacggtggg aacgacgact gaaatcaatg actatctgcg tctcctctac gcacgtgtgg
    12901 ggacgcctta ctgtatcaac ggacatggag ctatcaatgc ttcttctgtg gagcaaatcg
    12961 ttgataaggt tttggagtta cctgaacgcc agcgcttgca gatcttggct cctgtcatcc
    13021 gcaagaaaaa aggccaacat aagagtgtta tcgagaaggt tcagaaagac gggtatgttc
    13081 gtgttcgtgt ggatggggaa gtctatgatg tgaccgaagt gccagagttg tctaagagca
    13141 agcaacacaa tatcgatgtc gtggttgacc gtattgttat caaggagggc attcgtagcc
    13201 gtctctttga ttccattgaa gctgcccttc gtatcgcaga aggttatgtg attatcgaca
    13261 ccatggacga ctctgagttg ttgttctctg agcattatgc ctgtccagtt tgtggattta
    13321 ctgtcccaga gttagaacct cgtctcttct ctttcaatgc tccctttggc tcttgtagtg
    13381 agtgtgacgg cttggggatt aagctggagg tggatactga tttggtagtg ccagatgcca
    13441 gcaaaacctt acatgaggga gcgctggcac cttggaatcc tatctcatcc aactactatc
    13501 caaacatgtt agagcaggcc atgaaagtct ttggagtggc tatggataag ccttttgagg
    13561 acctgtcaga agaagataag aacttgattc tctacggctc agatggtaag gaattccatt
    13621 tccattatga aaatgaattt ggtggtgtgc gcgatatcga cattccgttt gagggagtta
    13681 tcaataatat caaacgtcgt taccatgaaa ccaatagcga ttacactcgc actcagatgc
    13741 gtctctacat gaatgagctg acctgcggaa cctgtcaagg ctatcgtctc aatgaccagg
    13801 ccttgtctgt ccgtgtgggc ggccagcaag ggccacatat tggagaaatc tcagacctgt
    13861 ctatcgctga ccacttggac ttggtgagcc agttaacttt gtctgaaaat gaagccatca
    13921 ttgctcgtcc cattctcaag gaaatcaagg atcgtttgac cttccttaat aatgtgggtc
    13981 ttaactatct aaccctgtca cgttcagcag gaaccctttc aggtggggaa agtcagcgca
    14041 ttcgtttggc aacccagatt ggttccaacc tatcaggtgt cctctatatc ctagacgagc
    14101 cgtcaatcgg tcttcaccag agggacaatg accgcctgat tgccagtctg aaaaagatgc
    14161 gtgacttggg caatactctt atcgtggtgg aacacgacga agataccatg cgtgaagctg
    14221 attatctgat tgacgttggt cctggtgccg gtgtttttgg tggtgagatt gttgcagcag
    14281 gtacacctaa gcaagtggct cgcaacagca agtctatcac aggtcagtac ttgtcaggca
    14341 aacgtgtcat cccagtacca gaagagcgcc gtgtcggaaa tggtcgtttt attgaagtga
    14401 caggagcgcg tgagaacaac ttgcaaaatg tcacggctcg ctttccacta ggaaaattta
    14461 tcgcagtgac aggtgtgtcg ggttcaggga aatcgactct aatcaacagc attctcaaaa
    14521 aagccattgc ccagaagctc aaccgtaatt cagacaaacc tggtaagttt aagactatta
    14581 cagggattga gcatgtcgac cgtttgattg atattgacca gagccctatc ggacggacgc
    14641 cgaggtctaa cccagctacc tatacaggag tttttgacga tatacgtgac ctttttgctc
    14701 agacaaatga agccaagatt cgaggctaca agaaggggcg cttcagtttc aacgtcaagg
    14761 gaggtcgctg tgaagcttgc tcaggtgacg ggattatcaa gattgagatg cacttcttgc
    14821 cagatgttta tgtggcttgt gaagtttgcc acggaacccg ctataacagt gaaaccctag
    14881 aagttcacta caaggaaaag aatatttcgc aggtcttgga tatgacggtc aacgatgcgg
    14941 tagaattttt ccaacacatt ccgaaaattc aacgcaaact ccagaccatc aaggatgtag
    15001 gtctaggcta tgtgaccttg ggacagccag ctaccaccct ttctggggga gaagcccaac
    15061 gtatgaagtt agctagcgaa ctccacaaac gctcgacagg aaaatctttc tacattctgg
    15121 atgagccgac gacagggctt catacagagg acattgctcg cctgctcaag gttttagctc
    15181 gctttgtaga cgccggcaat acagtcctcg tcatcgagca caatctggat gttatcaaga
    15241 cggcagacca tatcattgac ttgggacctg agggcggtgt cggtggtgga accatcatcg
    15301 taacaggaac tccagaagaa gtagctgcca acgaagccag ctatacagga cactatttga
    15361 aaggaaagtt acatcatgaa taaacgcgta caagcatttc tagctaaaat gcaagaaaaa
    15421 gaactagatg gtatcatcat caacaatctt aaaaacgtct attatttgac tggtttttgg
    15481 ggctcaaacg gaacagtctt tatcagccgt gaccgtcagg tcttagtgac agactctcgc
    15541 tatatcatcg cagctaagca agaaaccagt ggttttgaga ttgtggctga tcgtgatgaa
    15601 ttggctgtca ttgcaggaat tgttaaggac atgaacttga ctcgtatcgg ttttgaagat
    15661 gagatttcag tgtcttatta ccaccgtatg caggcagctt ttgcaggttt gaacttgctt
    15721 ccacaaactc agtttgtgga aggtcttcgt atgattaagg atgaggcaga gattgcagcg
    15781 attcgcaagg cttgttctat ctcagaccaa gctttccgcg atgcgcttga ctttatcaaa
    15841 ccaggaaaaa ctgaaattga gattgccaac ttccttgatt tccgcatgcg tgagttggga
    15901 gcatctggct tatcttttga tacgatccta gctagcggta tcaattcttc taaaccccat
    15961 gcccatccaa tgcacaaacc agtggagttg ggagaagcca ttaccatgga cttcggctgt
    16021 ctctatgacc actatgtcag tgatatgacc cggactatct atctagggca tgttagcgat
    16081 gagcaggcag agatttacaa tacggttcta aaagctaacc aagccttgat tgaccaagct
    16141 aaggcaggct taggtttccg tgactttgac aaaatccctc gtgatattat cattgaggca
    16201 ggttatggtg actactttac tcacggcatt ggccacggta ttggtctgga tatccatgag
    16261 gaaccctact ttagtcagac ttctacagaa actattaaga caggtatggc cttgaccgat
    16321 gaaccaggta tctatatcga aggcaaatat ggcgttcgta tcgaggatga tatcctgatt
    16381 acagagacag gttgtgaatt attgacccta gctccaaaag agttgatagt catttagttg
    16441 tttgtcaaga aaaaagtacg aaaatgacga aaaaagtcaa aaaaaattaa aaataggtcg
    16501 caagtcggat gttttttatg gtataataga ctaaactgat agtaacatgt agcgaaaggg
    16561 gtaggtacat gattaaaatt tatacagtct caagttgtac tagttgtaaa aaagcaaaaa
    16621 cctggctcaa tgcccaccag ttaagttata aagaacaaaa ccttggtaaa gaaggaatta
    16681 cgagagaaga attactggat attctaacca aaacagataa cggaatagcc agcatcgttt
    16741 cgtctaaaaa tcgctatgcc aaagcccttg gagtggatat tgaagatttg agtgtcaatg
    16801 aagttctcaa tctgattatg gaaacaccga gaattttaaa gagcccaatc cttgtagatg
    16861 aaaaacgcct gcaagttggc tacaaggaag acgatattcg tgccttccta ccacgctctg
    16921 tccgtaatgt agaaaatgca gaagcacgtt tgcgtgcagc tctataaaca tcaaggctgg
    16981 gagcacttcc cagtcttatt ctattttaat ttcaaaaaga aagaagaaag aaatgaaaaa
    17041 aatagttctt gttagtctag ctttcctttt tgtcctggtt ggttgcggac agaaaaaaga
    17101 aactggacca gctacaaaaa cagaaaaaga tacgcttcag tcggcattgc cagttattga
    17161 aaatgctgag aagaatacag ttgtaactaa gactttggtc ttgcccaagt cagatgatgg
    17221 tagccagcag acacaaacaa ttacttacaa agacaagact tttttgagtc tagctatcca
    17281 acaaaaacgt ccagtctctg atgagttgaa gacttatatt gaccaacatg gagtggagga
    17341 aactcaaaaa gctcttcttg aagcggagga gaaggataag tctatcattg aagctcgtaa
    17401 attggcaggt ttcaaacttg aaacaaaact attgagcgca acggaacttc aaacaacgac
    17461 tagttttgat tttcaagttc tggatgtcaa gaaggcttcc cagttggaac atctgaagaa
    17521 tattggtttg gaaaatcttt tgaaaaatga accaagcaaa tatatttcag atagattggc
    17581 aaatggcgcg acagaacaat agaaaatcca aataaataga ctgcctgaat tgtagaaggt
    17641 aaggaattat gctataatgg tactattcta aggaaagagg gtgttagaat gggatttact
    17701 gaagaaacag tacgttttaa attggacgat tccaataaaa aagaaattag cgaaactttg
    17761 acagatgttt atgcttcgtt gaacgataag ggttacaacc caattaacca aatcgtaggt
    17821 tacgtattga gtggagaccc tgcctacgtt cctcgttata ataatgcacg aaatcaaatc
    17881 cgtaagtatg agcgtgatga aatcgttgag gaattggttc gctactacct caaaggacaa
    17941 ggagtcgatc tataacctat gagaattatg ggattggacg tcggttcaaa aacggtaggg
    18001 gtggcgatta gcgatccgct tggttttaca gctcaagggc ttgaaatcat ccagataaat
    18061 gaagaacaag gccaatttgg ttttgaccgc gttaaggaat tggttgatac ttacaaggtg
    18121 gaacgatttg tagtgggctt gcctaaaaac atgaacaata caagtggacc gcgcgtagaa
    18181 gctagtcaag cctacggagc aaagctagaa gagttttttg gtttaccagt agactatcag
    18241 gatgaacgct tgacaacagt ggctgctgag cgcatgttga ttgaacaagc agatatcagt
    18301 cgcaataagc gcaagaaagt cattgataag ttagcagctc agctgatttt acaaaattat
    18361 ttagatagaa aattttaata taaaggagag gctatgtcac acgatcataa ccacgaccac
    18421 gaagaacgtg aactaatcac actagtagat gagcaaggaa atgaaacctt gtttgaaatc
    18481 cttttgacaa ttgatggaaa agaagaattt ggtaaaaact atgttcttct agtaccagtt
    18541 aacgcagaag aagacgaaga cggacaagtt gaaatccaag cttactcatt cattgaaaat
    18601 gaagatggaa cagaaggcga attgcaacca atcccagaag actcagaaga cgaatggaac
    18661 atgattgaag aagtcttcaa cagctttatg gaggagtgat acagtctggg agactgtatc
    18721 agcccaactc cagaaatggg gaagagttgg aacgtccagt gaacattttt accccaactc
    18781 ttagaaattg agaagagttg gaacatctag tggatgtttt tagcccatat tcacattcat
    18841 agtagctagt tattagctaa ccaggtaaga ggtcgggacg aaaatcctgg ccttgttttt
    18901 attagtaacc atactttaat gcgataattg attggacttt tgtcaaggag atgtgtatgt
    18961 ttgaagtaga agaatggctc catagtcgga ttggtttgaa ttttcgatca ggtttgggcc
    19021 gaatgcagca agcggtggat ttgttaggaa atcctgagca gtcttaccct attatccacg
    19081 taacagggac taatgggaaa ggatctacca ttgcttttat gagggaatta tttatggggc
    19141 atggcaaaaa agttgcgacc tttacctccc ctcatatcgt ctctatcaat gaccgaatct
    19201 gcattaatgg gcaacctata gcagacgcag actttatccg tttgactgat caggtcaagg
    19261 agatggagaa aacgcttctg caaactcctg cccagttgtc cttttttgaa ttgctgacct
    19321 tagttgcttt tctttatttt agggagcaag aggtggattt ggttttatta gaagtgggaa
    19381 ttggtggctt acttgacacg accaatgtgg taactggaga gcttgctgtc atcacctcca
    19441 ttgggcttga ccatcaagaa accttgggtg atagtctaga agcaattgca gagcagaaag
    19501 ctggtatttt caaggctggt aaaaaggcag tgattgcgaa attgcctcca gaagctagac
    19561 ttgtctgtca gaaaaaagcc gaatctttag ctgttgacct ttatcaggca ggtcaagatt
    19621 tttcagtgct gaatggtgat ttttcaagct ctttactaaa tctttcacag ctgaacatag
    19681 gcttagaagg agcctatcag caggagaatg cggccttggc gttgcaaact tttcttcttt
    19741 ttatgggaga aagaaaggaa gctattgatg aacaggctgt aagaaaggcc ttggaacaga
    19801 cccattgggc tggtcgcttg gagcgtattc gcccacagat ttacttggat ggtgctcata
    19861 acctccctgc cttgactcgc ttggttgagt ttatcaaaga aaaagagcag gaaggctatc
    19921 gacctcaaat cctctttgga tccttgaaac gtaaggatta tcaagggatg ttgggttatc
    19981 tgactgaaaa tttacctcaa gtggaactca aaatgaccgg ctttgactat caagggtctt
    20041 tggacgagac agatgtaaca ggttaccatg taattccctc ttaccgagaa tttatcagca
    20101 gtttcgaaga aagggcagac gctcaagact tgctgttcgt tacagggtct ctctatttta
    20161 tctcagaagt acggggctac ctgctggacc gtgagcagat aaattgaccc ccttttttga
    20221 acttgttata ctaggggaat tgctggttag agaaaatttc tctaaattgg tagcagaaag
    20281 gaaattcatc atgaaattaa aaagattcac actttctctt gcttctctag caagttttag
    20341 tctcttagta gcttgttcac aaagagctca acaggttcaa cagcctgttg ctcagcaggt
    20401 ccaacagcct gctcaacaga ataccaatac tgcaaatgca ggaggtaacc aaaatcaagc
    20461 ggctccagta caaaaccaac ctgttgctca accgaccgat attgatggga cttatactgg
    20521 tcaggatgac ggagaccgta tcactttagt ggtaactgga acgactggta catggactga
    20581 gctcgaatct gacggggatc agaaagtcaa acaggttaca tttgattcag caaatcaacg
    20641 catgattatt ggcgatgatg tcaaaattta cactgtaaac ggtaatcaaa tcgtcgtaga
    20701 tgatatggat agagacccat cggaccaaat cgttttaact aaataagact atgcaagtag
    20761 aaagggctgg atttttcagc ctttttactt ttacctagaa aataatcata aatacttgcc
    20821 ttctaccgaa tacctgcgtt atactagtat caattacttg tttttagcat tcaaactatc
    20881 aaggagggga tatgaaatat aggaaatttc aattattgat gtccaagtat ggctttagtc
    20941 tttcgattat gctacttgaa ctttgtcttg tttttggtct ctttctttat ttaggacgca
    21001 tggctcccat tttatggatt actgtcctca ttctactgag tatcatcaca atcatttcga
    21061 tagtcaaccg taatacgact cctgagaata aggtaacctg gttgttagta gcctttgtgc
    21121 cagtatttgg tcccttgctc tatctgatgt ttggtgaaag gcgattgtcc aaaaaagaaa
    21181 tcaaacaact gaagaagcta ggctctatgc atttccaaga agcaaatagc cagctactaa
    21241 aagagaaatt aaaagaaagt gacaaggcag cttatggagt catcaagtcc ttattgagta
    21301 tggataccaa tgctgacatc tatgatcaaa ctgcctctac attttttcct aacggagaag
    21361 ctatgtggaa aaagatggta gaagatctta aaaaggctga gaaatttatt ttcttggaat
    21421 attacattat agaagaaggt ttgatgtgga atcgcatact agatatacta gagcaaaagg
    21481 tagctcaggg tgtagaggtt aagatgctct atgatgatat cggctgtatg gctactttaa
    21541 caggagatta tgcacatcga cttcgtcagc tgggcatcga ggcccataaa ttcaataaag
    21601 ttattcctcg tttgacagtg gcttataata acagagatca tagaaaaata ttgattgttg
    21661 atggtcagat agcctatact ggtggggtca atctggcaga tgagtacatt aaccacgtcg
    21721 agagatttgg ttattggaag gatagtggaa ttcgcttaga cggactagca gtaaaagccc
    21781 tgacacgctt atttttgacc acttggtaca ttaatcgagg agaaattagt gattttgatc
    21841 aatatcattt agaaaatcat tctatcccga gtgacggttt aaccattcca tacggaagtg
    21901 gacccaagcc aatttttcga gcgcaggtag ggaaaaaagt ttatcagagt ttaatcaatc
    21961 aagcaacaga atcggtctat attacgacac cttatttgat tatagattat gatttaacag
    22021 agacaatcaa aaatgcagct atgagagggg tcgatgttcg aattatcacc ccttacatac
    22081 cagataagaa gttcattcag ttagtcacga gaggagctta tcccgacctt ctttctgctg
    22141 gtgttcggat ttatgagtat agtccaggtt ttattcatag taagcagatg ttggtagacg
    22201 aagattttgc ggtggtgggg acaatcaatc tcgactaccg gagcttggta caccattatg
    22261 aaaatgcagt cttactctat aaaactcctt ctataaggga aatcgcccga gattttcgaa
    22321 atatatttgc agattctcag gaagtctatc ctcattctat caaaacgagc tggtatcaaa
    22381 agcttgtaaa agaaatcgcc cagctattcg cccctatctt ataagaaatc tagaacttag
    22441 gatacaaacc agacctattt ttcttgtctt ttactaatag aaagaaagag gaggggaatt
    22501 catgctaact aaggaagaag tgaatgatat gattgagttc aaacagacac atttacaccg
    22561 ctttcgggaa attgaaacct ttgtgaaact atgcaaaaaa tcactcaaac agccatcgca
    22621 agctgacaat cctaggactt tttgatatac taagacagat aaattgaaca ggagaaacga
    22681 tatgagtgtg tatggtagag tagaagaagt tcataaggag aatcgtgaac ctctagaata
    22741 ccaaatcgaa caagaatcgc atcatcgtga atcaagtcgt cttcctttgg tgaaaatttt
    22801 actatggagt acgcttgtaa cggggataac tctaggagta ccgctattac tcgacttaat
    22861 gagtgcacaa gaagtgcagg atttttatgc aggttggggg cttcatcaga cagggaagat
    22921 ttacagcgac tattatggaa gtcaaggttt gctttattat ttgctgactt acgtgagtca
    22981 gggcggattt ttctttgcca tctttgagtg gttagccttg gtagcaggag gatttttcct
    23041 ttttagatca gcggacacct tgacagagca aggagaccaa gctggacatc tggtgactat
    23101 tttttacatg ctagttacag gtcttgcttt tggtggaggc tatgcgactc ttttagcgct
    23161 tcctttctta ttcgcagcct ttagtttagt tgcggcttac ctaagcaatc caagccatga
    23221 taagggattt gtacggattg ggctagcttt ggcaggcgga tttttctttg ctcccttatc
    23281 atcgctcctg tttattgctg tagtgagttt aggcttgttg gtctttaacc ttgggcatag
    23341 acgctttgcg catgggtttt atcagtttct tgcagtggct ttaggttttt cacttgtctt
    23401 ttatccaact gcctactata gtgctgcaac aggaagtttt ggggatgcga ttagtggtat
    23461 tcgttatcct attgacagta ttcgctttga ttttacttct aaaattttag agaatatgtt
    23521 tttttatggc ttgttgtccc ttggtttggg atttgtggtt tgtatctttt taggtctctt
    23581 tcaatccaaa ccctttaaac tatacgtcat ttcggttcct gcaagtcttg tggtaatttt
    23641 aggtttgatt ttacttttct tttcacaaga gcctctgcac gcttcttatt tgatggtcgt
    23701 cttccctgtt ttcctacttt tattggtaac caatattaag agtcaacaga gggggcgtag
    23761 tgctagaaga agccgaagag aaacgccagt tagcctatgg agtcgtttct tcaaaggaaa
    23821 tctatatctg ctagtttttg ggtttgtcta tcttttgtct gttccttttt tgatgaagtt
    23881 tgtcctttat ccagtacctt atcaagaacg taatcgtctt gctgatttgg taaaagagga
    23941 gacaaatacg gaagatgcta tctatgcatg ggatgatact gcgactcttt atcgtaagag
    24001 tgagcgcttg tcgccatcgg cgattttgtc cccgttgcac tatacagcaa ctgaggaaaa
    24061 tcgtaataag ttacttaatg acttgaaaga aaaacaacct aaggtgattg tggtaaatga
    24121 caaggtggta gtctggtctg aagtggaaac acttttaaaa gaaaattacc aacaagtaaa
    24181 gactgattac tcagagttta aagtctataa aattaaataa ccaaatcaat atcttgtgta
    24241 tttttaaaaa ttttaggatt tttaacacaa gatattgatt tttcttttta gagtggtata
    24301 atacttttta gaaagaacat tttagaaaag agcatgcgta tgattgcact agaagaaaaa
    24361 attacaattt tgccaactct cttcgtcgag aaacgagatg ggagacgtgt tgtatttgat
    24421 gtggacaaga ttgacaaggc tctccacaag gcggctgaca aggttatgga tgtgacaccc
    24481 ctggttgaaa aacgcctcaa tgatctgact gagcgaatta ttacagaaat tcatagtcgc
    24541 tttccacagg gaattaagat ttacgaaatt caaaatatcg tagaacatga actccttgaa
    24601 gccaaagaat atgcgctggc tgaggagtat attacttatc ggacacagag ggattttgag
    24661 cgctcaaaag cgacggatat caactttagt attcataaac ttctcaacaa agaccagaca
    24721 gttgtcaatg aaaatgctaa taaagacagt gatgtcttta acactcagcg tgatttgaca
    24781 gcagggattg ttgggaaatc aatcggactg caaatgcttc ctaagcacgt agccaatgcc
    24841 caccaaaagg gggatatcca ctatcacgat ttggactaca gtccctatac ccctatgacc
    24901 aactgctgtt tgattgattt taagggtatg ttggaaaatg gttttaagat tggaaatgca
    24961 gaggtagaga gtcccaagtc tatccagact gcgacagcac agatttctca aatcattgcc
    25021 aacgttgctt ctagccagta cggtggctgt tcagctgacc gtatcgatga aattttggcg
    25081 ccttatgcag agaagaatta tcaaaaacat ctcaaagatg cagaagagtg ggtattgcct
    25141 gaaaaacggg aagattacgc ttggaagaaa acgcaaaagg acatctacga tgccatgcaa
    25201 tctcttgagt atgaaatcaa tactctcttc acttcaaatg gacaaacacc ttttacttcg
    25261 ttaggttttg gtctgggaac cagtcgtttt gaacgagaaa ttcaaaaagc tattttaaac
    25321 attcgcatca agggtcttgg ttcagaacac cgtacggcta tctttcctaa acttattttc
    25381 actctgaaaa gaggccttaa tttagaggaa ggaactccca actacgacat caagcagttg
    25441 gctcttgagt gtgcaaccaa acggatgtat ccagatgttt tatcttacga taagattgtt
    25501 gacttgacag gatccttcaa ggtgcctatg ggttgccgtt ctttcctcca aggatggaag
    25561 gatgaaaatg gtgtagaagt caattcaggt cgcatgaatc tgggtgttgt gacggttaat
    25621 ctgcctcgta ttgctcttga gtctgaaggt gatatgaata agttctggga aatcttcaac
    25681 gagcgaatga atatcgcaga agatgctctt gtttaccgtg tcgaacgcac taaagaggcg
    25741 acaccagcaa atgctcctat tctttatcag tacggtgctt ttggccatcg tctaggtaaa
    25801 gaagaaagtg ttgaccagct ctttaagaat cgtcgtgcta ccgtttcgct gggctatatc
    25861 ggcttgtatg aagtagcgac agttttcttt ggtaacagct gggaaagtaa tccagatgct
    25921 aaggaattca cgctagacat cattcacgat atgaaacgcc gtgtagaaga gtggtcagac
    25981 caatatggct accatttctc tatctactca acaccatccg aaagtctgac agaccgtttc
    26041 tgtcgactag atatagacaa gtttggctct attcctgata tcacagacaa ggaatactac
    26101 accaactctt tccactacga tgttcgtaaa aatccaacac cgtttgaaaa attggacttt
    26161 gagaaagtct atccagaagc aggtgcgtca ggtggtttca tccattattg tgagtatcca
    26221 gtccttcagc aaaatccaaa ggccttggaa gctgtctggg attatgctta tgaccgtgta
    26281 ggctatctag gcaccaatac tccgattgac cgttgctaca agtgtgactt tgaaggggat
    26341 tttgaaccaa ctgagagagg gtttgcttgt ccaaactgtg gcaatagcga ccctaaaaca
    26401 gtagatgtgg tgaagcgaac ttgtggctac ctaggtaatc ctcaagcaag accgatggtc
    26461 aacggccgtc acaaggaaat cgctgcgcgt gtcaaacata tgaatggttc aacgattaaa
    26521 atagctgggc atcaagtaac aaattagaaa gaaatgaaat gggaaaatat caactagacg
    26581 ataaggggcg cgcacaagtg acccgttatc acgagaaaca ctctaaaggt ggagctggta
    26641 agaaagaacg cttgcttagc ttcagagaac aatttttaaa caagaacaag aaaaaataaa
    26701 agtgagagcc agctctcgct tttctcatag tgggaggtaa ggatggaatt acgcagacca
    26761 agattagcgg ataagaaagc tgttttagat atgatgacag agtttgaaaa gagccaatca
    26821 gcccatgatg gaggattttg ggatacagag aattttgtgt atgaagagtg gttggaaagc
    26881 aatcaggaac aggaaatggg gattaatctg cctgaaggat gggttcctgc aattcagtta
    26941 gtggcttttt ctgagaaagg tcaagcagtt ggatttctta atctccggtt gcgcctcagt
    27001 aactttttac tagaagaagg tgggcacatt ggctactcca tccgtccatc tgaaagaggc
    27061 aagggttatg caaaagagac tctccgtcag ggcttgcaag ttgctaagga aaagaacatc
    27121 aagaaagctc tggtgacctg tagcgtgaat aatcctgcta gcagagcagt cattctagca
    27181 aatggtggaa tatttgagga tgctcacaat ggagtcgagc gttattggat agaggtagcg
    27241 aatgaataat ccaaaaccac aagaatggaa aagcgaggaa cttagtcaag gtcgtatcat
    27301 tgactacaag gcctttaact ttgtggacgg cgaaggcgtg cgtaactctc tctatgtatc
    27361 aggctgtatg tttcactgcg agggatgtta taatgttgcg acttggtctt ttaatgctgg
    27421 cattccctat acagcagaat tagaagagca gatcatggca gatcttgctc agccctatgt
    27481 tcaaggtttg actttgttgg gaggggagcc ttttcttaat actgggattc tcttgccact
    27541 tgttaagcgg attcggaagg aattgccaga caaggacatc tggtcctgga ccggctacac
    27601 ttgggaagaa atgatgttgg aaactccaga taaactggaa ttcttgtcac tgattgacat
    27661 tcttgtcgat ggaagatatg atcgaactaa gagaaatctt atgctccagt ttcgaggttc
    27721 atctaaccaa cgaattatcg atgtgcaaaa atcgctcaaa agtgggcaag tagtgatttg
    27781 ggacaagctc aatgacggaa aagaaagcta tgaacaggtg aagagagaat gaagaaaaaa
    27841 gacttagtag accaactagt ctcagagatc gagacgggga aagtcaggac actgggaata
    27901 tacggtcatg gagcttcagg taaatcaacc tttgcacagg aattgtacca agctttagat
    27961 tctactacag taaatttgct agaaacagat ccctatatca cctccggacg ctatttggta
    28021 gtacccaagg acgcgccgaa tcaaaaggtg acagccagtc tgccagtggc gcatgaactg
    28081 gagagtttgc agagagatat ccttgccttg caggcgggta tggatgtctt gacaattgaa
    28141 gaaccttgga aggctagtga ggtcttgtct ggagccaaac caattttgat tgtcgaaggg
    28201 atgtctgttg gttttctacc caaggaactc tttgaaaaaa ccatctgttt ctacacggat
    28261 gaggagaccg aattaaagcg acgccttgct agagatacga ctgtgagaaa tcgcgatgca
    28321 tcctttatat tggctagcca tcagatgaga cgggagcagt atctgcgata ctataaagaa
    28381 actgagtcta aggcggatat cttagtggac caatcagaag ataaatttga tgtcaagagg
    28441 acttaactta tgtatagaag aaatatagaa gaaaatccct aattttagaa agaaaaagta
    28501 ggaaaacagt ttcatcccaa aaaaacgaaa aaacctaaca aatcccttgc aatcgcaggg
    28561 gatttgtgtt attctattat ggtgctgtaa attacagcct tagttttgat gcaagaggtt
    28621 gcgacacgct cggttgcatt gccacgcaac accgcgtcgg ttttcttgtg gagctagcct
    28681 attatcttaa atagacgaaa aggagaaaaa gatggcaaac aaaaaaatcc gtatccgttt
    28741 gaaagcttac gaacaccgta cgcttgacac agcggctgca aaaatcgtag aatcagctac
    28801 tcgtacaggt gcacaagttg cgggtccaat cccacttcca actgaacgta gcctctacac
    28861 aatcattcgt gcgactcaca aatacaaaga ctctcgcgaa caatttgaaa tgcgtacaca
    28921 caaacgtttg atcgatatcg ttaacccaac tcaaaaaaca gttgatgcct tgatgaaatt
    28981 ggatcttcca agtggtgtaa acgtagaaat caaactttaa tctaaaatat aaaagagcag
    29041 aggctggtgt ttcaatctaa ttgaacacgg gctagactcg gtgtgaaaaa gataaacttc
    29101 ctattgtccg ctagacactg cgttagtttc ctattttcac tttgagtttg acgccctttg
    29161 tatcttagac ttgagcataa aaaacgctcg ttaaaaactt tttgaataaa aaatatagaa
    29221 aaggaactat tttctcatga caaaaggaat cttagggaaa aaagtgggaa tgactcaaat
    29281 cttcactgaa gctggcgaat tgatccctgt aacagttatt gaagcaactc caaacgttgt
    29341 tcttcaagtt aaaactgttg aaacagacgg atacaacgct atccaagttg gtttcgatga
    29401 caaacgcgaa gtattgagca acaaacctgc taaaggacat gtagcgaaag ctaacacggc
    29461 tcctaagcgc ttcattcgtg aattcaaaaa cgttgaaggc ttggaagttg gtgctgaaat
    29521 tacagttgaa acattcgcag ctggagatgt tgttgatgta actggtactt ctaaaggtaa
    29581 aggtttccaa ggtgttatca agcgccacgg acaatcacgt ggaccgatgg ctcacggttc
    29641 tcgttaccac cgtcgtccag gttctatggg acctgttgca cctaaccgcg tattcaaagg
    29701 taaaaacctt gcaggacgta tgggtggtga ccgcgtaaca attcaaaatc ttgaagttgt
    29761 acaagttgtt ccagaaaaga acgttatcct tatcaaaggt aacgtaccag gtgctaagaa
    29821 atctcttatc actatcaaat cagcagttaa agctggtaaa taataaagaa aggggaaatc
    29881 agtcacaatg gcaaacgtaa cattatttga ccaaactggt aaagaagctg gccaagttgt
    29941 tcttaacgat gcagtatttg gtatcgaacc aaatgaatca gttgtgtttg atgtaatcat
    30001 cagccaacgc gcaagccttc gtcaaggaac acacgctgtt aaaaaccgct ctgcagtatc
    30061 aggtggtgga cgcaaaccgt ggcgtcaaaa aggaactgga cgtgctcgtc aaggttctat
    30121 ccgctcacca caatggcgtg gtggtggtgt tgtcttcgga ccaactccac gttcatacgg
    30181 ctacaaactt ccacaaaaag ttcgtcgcct agctcttaaa tcagtttact ctgaaaaagt
    30241 tgctgaaaac aaattcgtag ctgtagacgc tctttcattt acagctccaa aaactgctga
    30301 atttgcaaaa gttcttgcag cattgagcat cgattctaaa gttcttgtta tccttgaaga
    30361 aggaaatgaa ttcgcagctc tttcagctcg taaccttcca aacgtgaaag ttgcaactgc
    30421 tacaactgca agtgttcttg acatcgcaaa tagcgacaaa cttcttgtca cacaagcagc
    30481 tatctctaaa atcgaggagg ttcttgcata atgaatttgt atgatgttat caaaaaacct
    30541 gtcatcactg aaagctcaat ggctcaactt gaagcaggga aatatgtatt tgaagttgac
    30601 actcgtgcac acaaactttt gatcaagcaa gctgttgaag ctgctttcga aggtgttaaa
    30661 gttgccaatg ttaacacaat caacgtaaaa ccaaaagcta aacgtgttgg acgttacact
    30721 ggttttacta acaaaactaa aaaagctatc atcacactta cagctgattc taaagcaatc
    30781 gagttgtttg ctgctgaagc tgaataatct aaggaggaaa tatcgtggga attcgtgttt
    30841 ataaaccaac aacaaacggt cgccgtaata tgacttcttt ggatttcgct gaaatcacaa
    30901 caagcactcc tgaaaaatca ttgcttgttg cattgaagag caaggctggt cgtaacaaca
    30961 acggtcgtat cacagttcgt caccaaggtg gtggacacaa acgtttctac cgtttggttg
    31021 acttcaaacg taataaagac aacgttgaag cagttgttaa aacaatcgag tacgatccaa
    31081 accgttctgc aaacatcgct cttgtacact acactgacgg tgtgaaagca tacatcatcg
    31141 ctccaaaagg tcttgaagta ggtcaacgta tcgtttcagg tccagaagca gatatcaaag
    31201 tcggaaacgc tcttccactt gctaacatcc cagttggtac tttgattcac aacatcgagt
    31261 tgaaaccagg tcgtggtggt gaattggtac gtgctgctgg tgcatctgct caagtattgg
    31321 gttctgaagg taaatacgtt cttgttcgtc ttcaatcagg tgaagttcgt atgattcttg
    31381 gaacttgccg tgctacagtt ggtgttgtcg gaaacgaaca acatggactt gtaaaccttg
    31441 gtaaagcagg acgtagccgt tggaaaggta tccgcccaac agttcgtggt tctgtaatga
    31501 accctaacga tcacccacac ggtggtggtg aaggtaaagc accagttggt cgtaaagcac
    31561 catctactcc atggggcaaa cctgctcttg gtcttaaaac tcgtaacaag aaagcgaaat
    31621 ctgacaaact tatcgttcgt cgtcgcaacg agaaataata ttaaactagt cgcttaagca
    31681 actagtaaat ccgccagctc ggtagcgctc cataggagtg caagccgctg tggtacaaca
    31741 tttaaaggag aaaatataaa aatgggacgc agtcttaaaa aaggaccttt cgtcgatgag
    31801 catttgatga aaaaagttga agctcaagct aacgacgaaa agaaaaaagt tattaaaact
    31861 tggtcacgtc gttcaacgat cttcccaagt ttcattggtt acactattgc agtttatgac
    31921 ggacgtaaac acgtacctgt ttacatccaa gaagacatgg taggccacaa acttggtgaa
    31981 tttgcaccaa ctcgtactta caaaggtcac gctgcagacg acaagaaaac acgtagaaaa
    32041 taaggagaac ataaatggca gaaattactt cagctaaagc aatggctcgt acagtacgtg
    32101 tttcacctcg taaatcacgt cttgttcttg ataacatccg tggtaaaagc gtagccgatg
    32161 caatcgcaat cttgacattc actccaaaca aagctgctga aatcatcttg aaagttttga
    32221 actcagctgt agctaacgct gaaaacaact ttggtttgga taaagctaac ttggtagtat
    32281 ctgaagcatt cgcaaacgaa ggaccaacta tgaaacgttt ccgtccacgt gcgaaaggtt
    32341 cagcttcacc aatcaacaaa cgtacagctc acatcactgt agctgttgca gaaaaataag
    32401 gaggtaaaat cgtgggtcaa aaagtacatc caattggtat gcgtgtcggc atcatccgtg
    32461 attgggatgc caaatggtat gctgaaaaag aatacgcgga ttaccttcat gaagatcttg
    32521 caatccgtaa attcgttcaa aaagaacttg ctgacgcagc agtttcaact attgaaatcg
    32581 aacgcgcagt aaacaaagtt aacgtttcac ttcacactgc taaaccaggt atggttatcg
    32641 gtaaaggtgg tgctaacgtt gatgcactcc gtgcaaaact taacaaattg actggaaaac
    32701 aagtacacat caacatcatc gaaatcaaac aacctgattt ggatgctcac cttgtaggtg
    32761 aaggaattgc tcgtcaattg gagcaacgtg ttgctttccg tcgtgcacaa aaacaagcaa
    32821 tccaacgtgc aatgcgtgct ggagctaaag gaatcaaaac tcaagtatca ggtcgtttga
    32881 acggtgcaga tatcgcccgt gctgaaggat actctgaagg aactgttccg cttcacacac
    32941 ttcgtgcaga tatcgattac gcttgggaag aagcagatac tacatacggt aaacttggtg
    33001 ttaaagtatg gatctaccgt ggtgaagttc ttccagctcg taaaaacact aaaggaggta
    33061 aataaccaat gttagtacct aaacgtgtta aacaccgtcg tgagttccgt ggaaaaatgc
    33121 gcggtgaagc aaaaggtgga aaagaagtag cattcggtga atacggtctt caagctacaa
    33181 ctagccactg gatcactaac cgccaaatcg aagctgctcg tatcgccatg actcgttaca
    33241 tgaaacgtgg tggtaaagtt tggattaaaa tcttcccaca caaatcatac actgctaaag
    33301 ctatcggtgt gcgtatggga tctggtaaag gggcacctga aggttgggta gcaccagtta
    33361 aacgtggtaa agtgatgttc gaaatcgctg gtgtatctga agagattgca cgtgaagcgc
    33421 ttcgacttgc tagccacaaa ttgccagtta aatgtaaatt cgtaaaacgt gaagcagaat
    33481 aaggagaagg catgaaactt aatgaagtaa aagaatttgt taaagaactt cgtggtcttt
    33541 ctcaagaaga actcgcgaag cgcgaaaacg aattgaaaaa agaattgttt gaacttcgtt
    33601 tccaagctgc tactggtcaa ttggaacaaa cagctcgctt gaaagaagtt aaaaaacaaa
    33661 tcgctcgtat caaaacagtt caatctgaag cgaaataata gactagggaa ggagaaattt
    33721 caatggaacg caataatcgt aaagttcttg ttggacgtgt tgtatctgac aaaatggaca
    33781 agacaatcac agttgtagtt gaaacaaaac gtaaccaccc agtctatggt aaacgtatta
    33841 actactctaa aaaatacaaa gctcatgatg aaaacaatgt tgccaaagaa ggcgatatcg
    33901 tacgtatcat ggaaactcgc ccgctttcag ctacaaaacg tttccgtctt gtagaagttg
    33961 ttgaagaagc ggtcatcatc taatcaaacc tgaaaggaga aaactgaaat gattcaaaca
    34021 gaaactcgtt tgaaagtcgc agacaacagc ggtgctcgcg aaatcttgac tatcaaagtt
    34081 cttggtggtt caggacgtaa atttgcaaac atcggtgatg ttatcgtggc atctgtaaaa
    34141 caagctactc ctggtggtgc ggttaaaaaa ggtgacgttg ttaaagcagt tatcgttcgt
    34201 actaaatcag gtgctcgtcg tgctgatggt tcatacatca aatttgacga aaacgcagca
    34261 gttatcatcc gtgaagacaa aactcctcgc ggaacacgta tctttggccc agttgcacgt
    34321 gaattgcgtg aaggtggctt catgaagatc gtgtcacttg ctccagaagt actttaattt
    34381 ttaggaacaa actagtcccc tagcttcaag ctagggtgcc cttatgggcg taagaaaaat
    34441 caaggagaaa cctaatgttt gtaaaaaaag gcgacaaagt tcgcgtaatc gctggtaaag
    34501 ataagggaac agaagctgtt gtccttactg cccttccaaa agtaaacaaa gttatcgttg
    34561 aaggtgttaa cattgttaag aaacaccaac gtccaactaa cgagcttcct caaggtggta
    34621 tcatcgagaa agaagcagct atccacgtat caaacgttca agttttggac aaaaatggtg
    34681 tagctggtcg tgttggatac aaatttgtag acggtaaaaa agttcgctac aacaaaaaat
    34741 caggcgaagt gcttgattaa tcacgaagga aaggagaagt ataatggcaa atcgtttaaa
    34801 agaaaaatat cttaatgaag tagttcctgc tttgacagaa caattcaact actcatcagt
    34861 gatggctgtg cctaaagtag ataagattgt tttgaacatg ggtgttggtg aagctgtatc
    34921 aaacgctaaa agccttgaaa aagctgctga agaattggca cttatctcag gtcaaaaacc
    34981 acttatcact aaagctaaaa aatcaatcgc cggcttccgt cttcgtgaag gtgttgcgat
    35041 cggtgcaaaa gttacccttc gtggtgaacg tatgtacgaa ttcttggata aattggtatc
    35101 agtttcactt ccacgtgtac gtgactttca cggtgttcca acaaaatcat ttgatggacg
    35161 cgggaactac acacttggtg tgaaagaaca attaatcttc ccagaaatca acttcgatga
    35221 cgttgacaaa actcgtggtc ttgacatcgt tatcgtaaca actgctaaca ctgacgaaga
    35281 gtcacgtgca ttgcttacag gccttggaat gccttttgca aaataatata ggaggtaaat
    35341 ctaatggcta aaaaatcaat ggtagctaga gaggctaaac gccaaaaaat tgttgaccgt
    35401 tatgctgaaa aacgtgctgc attaaaggcg gcaggggact acgaaggttt atctaaatta
    35461 cctcgcaacg cctcaccgac tcgtttacat aatcgttgta gagttacggg gcgcccacat
    35521 tcagtttacc gcaaatttgg tctgagtcgt atcgcttttc gcgaacttgc gcataaaggt
    35581 caaattcctg gtgtaacaaa agcatcttgg taatttaaga tatcaagggt gtcaaaactc
    35641 caagtaaaaa taggaaactt gacgaagaaa ctaaagtttc taggaaagtt tatctttttc
    35701 acacagagtt tagcccgggt tcaattgggc ttgccaattt gaacccgagc tacagctttg
    35761 gcaaaaaaga ccaatttgct ttggagcatt gcttctgcat taaattgtct atttttgctc
    35821 gtgctgttac gctctttgta tcatgtatta actagcaagt gcaacttgca aactactagt
    35881 aagaggagaa aaacaaaatg gttatgactg acccaatcgc agacttccta actcgtattc
    35941 gtaatgctaa ccaagctaaa cacgaagtac ttgaagtacc tgcatcaaac atcaaaaaag
    36001 ggattgctga aatccttaaa cgcgaaggtt ttgtaaaaaa cgttgaaatc atcgaagatg
    36061 acaaacaagg cgtcatccgt gtatttctta aatacggacc aaatggtgaa aaagttatca
    36121 ctaacttgaa acgtgtttct aaaccaggac ttcgtgtcta caaaaaacgt gaagaccttc
    36181 caaaagttct taacggactt ggaattgcca tcctttcaac ttctgaaggt ttgcttactg
    36241 ataaagaagc acgccaaaag aatgttggtg gtgaggttat agcttacgtt tggtaaaatc
    36301 aagatacaaa gctcgtaaag aacaaagcaa aattaggaag ttggagaagt ttgtttacaa
    36361 acaagccaac ttatctattt tgcacagttc ttagagcgtg ttcagttcag ctcttgaact
    36421 aaataagtat ctgaaccccg tgaaaactgg ccgttctggc ctgacaattt aacaggagaa
    36481 aataaacatg tcacgtattg gtaataaagt tatcgtgttg cctgctggtg ttgaactcgc
    36541 taacaatgac aacgttgtaa ctgtaaaagg acctaaagga gaacttactc gtgagttctc
    36601 aaaagatatt gaaatccgtg tggaaggtac tgaaataact cttcaccgtc caaacgattc
    36661 aaaagaaatg aaaactatcc acggaactac tcgtgccctt ttgaacaaca tggttgttgg
    36721 tgtatcagaa ggattcaaga aagaacttga aatgcgtggg gttggttacc gtgcacagct
    36781 tcaaggatct aaacttgttt tggctgttgg taaatctcat ccagacgaag ttgaagctcc
    36841 agaaggaatt acttttgaac ttccaaaccc aacaacaatc gttgttagcg gaatttcaaa
    36901 agaagtagtt ggtcaaacag ctgcttacgt acgtagcctt cgttcaccag aaccatataa
    36961 aggtaaaggt atccgttacg ttggtgaatt cgttcgccgt aaagaaggta aaacaggtaa
    37021 ataatgttga gtggttgatc atcaaccacc aacctatttt ccaactttgt gcatagcaca
    37081 cgatttaaaa ctaaagaggt gaaaactgtg atttcaaaac cagataaaaa caaactccgc
    37141 caaaaacgcc accgtcgcgt tcgcggaaaa ctctctggaa ctgctgatcg cccacgtttg
    37201 aacgtattcc gttctaatac aggcatctac gctcaagtga ttgatgacgt agcgggtgta
    37261 acgctcgcaa gtgcttcaac tcttgataaa gaagtttcaa aaggaactaa aactgaacaa
    37321 gccgttgctg tcggtaaact cgttgcagaa cgtgcaaacg ctaaaggtat ttcagaagtg
    37381 gtgttcgacc gcggtggata tctatatcac ggacgtgtga aagctttggc tgatgcagct
    37441 cgtgaaaacg gattgaaatt ctaataggag gacactagaa aatggcattt aaagacaatg
    37501 cagttgaatt agaagaacgc gtagttgctg tcaaccgtgt tacaaaagtt gttaaaggtg
    37561 gacgtcgtct tcgtttcgca gctcttgttg ttgttggtga ccacaatggt cgcgtaggat
    37621 ttggtactgg taaagctcaa gaagttccag aagcaatccg taaagcagta gatgatgcta
    37681 agaaaaactt gatcgaagtt cctatggttg gaacaacaat cccacacgaa gttctttcag
    37741 aattcggtgg agctaaagta ttgttgaaac ctgctgtaga aggttctgga gttgccgctg
    37801 gtggtgcagt tcgtgccgtt gtggaattgg caggtgtggc agatattaca tctaaatcac
    37861 ttggttctaa cactccaatc aacattgttc gtgcaactgt tgaaggtttg aaacaattga
    37921 aacgcgctga agaaattgct gcccttcgtg gtatttcagt ttctgatttg gcataagaaa
    37981 ggggataaaa tggctcaaat taaaattact ttgactaagt ctccaatcgg acgcattcca
    38041 tcacaacgta aaactgttgt agcacttgga cttggcaaat tgaacagctc tgttattaaa
    38101 gaagataacg ctgctatccg tggtatgatt acagcagtat ctcacttagt aacagttgaa
    38161 gaagtaaact aatgaagttt taggggatgt gcactgtacc atcccctaaa actagatata
    38221 gtcatctatg atgacatcgt ataggcgagt tgatggggga gacaaccttt tctcccttat
    38281 cggcgctagc attttacaaa agaggagaaa ataaaaatga aacttcatga attgaaacct
    38341 gcagaaggtt ctcgtaaagt acgtaaccgc gttggtcgtg gtacttcatc aggtaacggt
    38401 aaaacatctg gtcgtggtca aaaaggtcaa aaagctcgta gcggtggcgg agttcgcctt
    38461 ggttttgaag gtggacaaac tccattgttc cgtcgtcttc caaaacgtgg attcactaac
    38521 atcaacgcta aagaatacgc aattgtgaac cttgaccaat tgaacgtctt tgaagatggt
    38581 gctgaagtaa ctccagttgt tcttatcgaa gcaggaattg ttaaagctga aaagtcaggt
    38641 attaaaattc ttggtaacgg tgagttgact aagaaattga ctgtgaaagc agctaaattc
    38701 tctaaatcag ctgaagaagc tatcactgct aaaggtggtt cagtagaagt catctaagag
    38761 aggtgaccta tgttttttaa attattaaga gaagctctta aagtcaagca ggttcgatca
    38821 aaaattttat ttacaatttt tatcgttttg gtctttcgta tcggaactag cattacagtt
    38881 cctggtgtga atgccaatag cttgaatgct ttaagtggat tatccttctt aaacatgttg
    38941 agcttggtgt cggggaatgc cctaaaaaac ttttcgattt ttgccctagg agttagtccc
    39001 tatatcaccg cttctattgt tgtccaactc ttgcaaatgg atattttacc caagtttgta
    39061 gagtggggta aacaagggga agtaggtcga agaaaattga atcaagctac tcgttatatt
    39121 gctctagttc tcgcttttgt gcaatctatc gggattacag ctggttttaa taccttggct
    39181 ggagctcaat tgattaaaac tgctttaact ccacaagttt ttctgacgat tggtatcatc
    39241 ttaacagctg gtagtatgat tgtcacttgg ttgggtgagc aaattacaga taagggatac
    39301 ggaaacggtg tttccatgat tatctttgcc gggattgttt cctcaattcc agagatgatt
    39361 cagggcatct atgtggacta ctttgtgaac gtcccaagta gccgtatcac ttcatctatc
    39421 attttcgtaa tcattttgat tattactgta ttgttgatta tttactttac aacttatgtt
    39481 caacaagcag aatacaaaat tccaatccaa tatactaagg ttgcacaagg tgctccatct
    39541 agctcttacc ttccgttaaa ggtaaaccct gctggagtta tccctgttat ctttgccagt
    39601 tcgattactg cagcgcctgc ggctattctt cagtttttga gtgccacagg tcatgattgg
    39661 gcttgggtaa gggtagcaca agagatgttg gcaactactt ctccaactgg tattgccatg
    39721 tatgctttgt tgattattct ctttacattc ttctatacgt ttgtacagat taatcctgaa
    39781 aaagcagcag agagcctaca aaagagtggt gcctatatcc atggagttcg tcctggtaaa
    39841 ggtacagaag aatatatgtc taaacttctt cgtcgtcttg caactgttgg ttccctcttc
    39901 cttggtgtga tttccatttt accgattgca gctaaagatg tatttggtct ttctgatgtt
    39961 gttgcctttg gtggaacaag tctcttgatc attatctcta caggtatcga aggaatcaag
    40021 caattggaag gttacctatt gaaacgtaag tatgttggtt tcatggacag aacagaataa
    40081 aagtatttac tgaatcagta aatactgagg gagtggaggt ttaaactctg acatttgtaa
    40141 gagttggatc tcccctcttc tattttgttt ttaaatcggg gtgaaaagac tttttgcttc
    40201 tatttaaaaa taaaataagg agatcaaatc atgaatcttt tgattatggg cttacctggt
    40261 gcaggtaagg gaactcaagc agcaaaaatc gtagaacaat tccatgttgc acatatctca
    40321 acaggtgata tgttccgcgc tgcaatggca aatcaaactg aaatgggtgt tcttgctaag
    40381 tcatatattg acaagggtga attggttcct gacgaagtta caaatggaat cgtaaaagaa
    40441 cgcctttcac aagatgatat taaagaaaca ggattcttat tggatggtta cccacgtaca
    40501 attgaacaag ctcatgcctt ggacaaaaca ttggctgaac ttggcattga actagaaggt
    40561 attatcaata ttgaagtgaa ccctgacagc ctcttggaac gtttgagtgg ccgtatcatc
    40621 caccgcgtaa ctggagaaac tttccacaag gtctttaacc caccagttga ctataaagaa
    40681 gaagattact accaacgtga agatgataag cctgagacag taaaacgtcg tttggatgtt
    40741 aatattgctc aaggagaacc aatcattgct cactaccgtg ccaaaggttt ggttcatgac
    40801 atcgaaggta atcaagatat caatgatgtc ttctcagata ttgaaaaagt attgacaaat
    40861 ttgaaataaa gcgtttttca cacttgcaaa aatccgctac aaatgttata ctgaaatagt
    40921 ctgacttata attgttgtct ctgtgtctag aggcatcgaa tcgaaattta tggaggtgct
    40981 tttgcgtggc aaaagacgat gtgattgaag ttgaaggcaa agtagttgat acaatgccga
    41041 atgcaatgtt tacggttgaa cttgaaaatg gacatcagat tttagcaaca gtttctggta
    41101 aaattcgtaa aaactatatt cgtattttag cgggagatcg tgttactgtc gaaatgagtc
    41161 catatgactt gacacgtgga cgtatcactt accgctttaa ataatcgaaa aacttggagg
    41221 gataagaaat gaaagtaaga ccatcggtca aaccaatttg cgaatactgt aaagttattc
    41281 gtcgtaatgg tcgtgttatg gtaatttgcc cagcaaatcc aaaacacaaa caacgtcaag
    41341 gataagatag aaaggagaaa acatggctcg tattgctgga gttgatattc caaatgacaa
    41401 acgcgtagta atctcattga cttatgttta tggtatcgga cttgcaacat ctaagaaaat
    41461 tttggctgct gctggaatct cagaagatgt tcgtgtacgt gatcttacat cagatcaaga
    41521 agatgctatc cgtcgtgaag tggatgcaat caaagttgaa ggtgaccttc gtcgtgaagt
    41581 aaacttgaac atcaaacgtt tgatggaaat cggttcatac cgtggtatcc gtcaccgtcg
    41641 tggacttcct gtccgtggac aaaatactaa aaacaacgct cgcactcgta aaggtaaagc
    41701 tgttgcgatt gctggtaaga aaaaataata taggaggtaa aagtcttggc taaaccaaca
    41761 cgtaaacgtc gtgtgaaaaa gaatatcgaa tctggtattg ctcatattca cgctacattt
    41821 aataacacta ttgttatgat tactgatgtg catggtaatg caattgcttg gtcatcagct
    41881 ggtgctcttg gtttcaaagg ttctcgtaaa tctacaccat tcgctgctca aatggcttct
    41941 gaagctgctg ctaaatctgc acaagaacac ggtcttaaat cagttgaagt tactgtaaaa
    42001 ggtccaggtt ctggtcgtga gtcagctatt cgtgcgcttg ctgccgctgg tcttgaagta
    42061 acagcaattc gtgatgtgac tccagtgcca cacaatggtg ctcgtcctcc aaaacgtcgc
    42121 cgtgtataat catcgcatta cactgctttt cgtttaagag ggagtaacta aatgatcgag
    42181 tttgaaaaac caaatataac aaaaattgat gaaaataaag attatggcaa gtttgtaatc
    42241 gaaccacttg aacgtggcta cggtacaact cttggtaact ctcttcgtcg tgtacttcta
    42301 gcttctctac caggagcagc tgtgacatct atcaacattg atggtgtgtt acatgagttt
    42361 gacacagttc caggtgttcg tgaagacgtt atgcaaatca ttctgaacat taaaggaatt
    42421 gcagtgaaat cgtacgttga agacgaaaaa atcatcgaac tggatgttga aggtcctgct
    42481 gaagtaacag ctggtgacat tttgacagat agcgatattg aaattgtaaa tccagatcat
    42541 tatctcttta caattggtga aggttcttct ctaaaagcga ctatgactgt taacagtggt
    42601 cgtggatatg tacctgctga tgaaaataaa aaggataatg caccagttgg aacacttgct
    42661 gtagattcta tttatacacc agttacaaaa gtcaactatc aagtggaacc tgctcgtgta
    42721 ggtagcaatg atggtttcga caaattaacc cttgaaatct tgacaaatgg aacaattatt
    42781 ccagaagatg ctttagggct ttcagcacgt attttgacag aacatcttga tttgtttaca
    42841 aatcttactg agattgctaa gtcaactgaa gtgatgaaag aagctgatac tgaatctgac
    42901 gaccgtattt tagatcgtac gattgaggaa ctggacttgt ctgtgcgttc atacaactgt
    42961 ttaaaacgtg ccggtatcaa tactgtgcat gatttgacag aaaaatctga agcagagatg
    43021 atgaaagtac gaaatcttgg acgcaagagt ttggaagaag tgaaactcaa actcattgat
    43081 ttgggtcttg gattaaaaga taaataaagg aggaatacat ggcttaccgt aaactaggac
    43141 gcactagctc acaacgtaaa gcaatgcttc gcgatttgac aactgacctt ttgatcaacg
    43201 aatcaatcgt gacaactgaa gctcgtgcta aagaaatccg taaaactgtt gaaaaaatga
    43261 ttactctagg taaacgtggt gatttgcatg cacgtcgtca agcagctgct ttcgtacgta
    43321 atgaaatcgc atctgaaaac tatgatgaag caactgataa gtacacttct actacagcac
    43381 ttcaaaaatt gttctcagaa atcgcacctc gttatgctga acgtaacggt ggatacactc
    43441 gtatccttaa aactgaacca cgtcgtggtg atgcagcgcc aatggcgatc atcgaattag
    43501 tataaaatca tcaattttgt tgagtgttat gatgatggag tcttgtgctc ttagtctagc
    43561 tctggtctac cgctaggatt tcggtcctag cgggaacact catcataagt tgggatagta
    43621 gacgcttgtt tacgaaattg tttttttctt aagaacaact tcgtaagcag gcgtttttga
    43681 gtattttcgt tagaattatg ctatactatt tgaaaagaat cctgtttaat gttaaggttt
    43741 cttattttaa gaagaattgg agtttactta tgaaagccat tataactgtt gttggtaaag
    43801 ataaatctgg aattgttgca ggtgtttctg gtaaaattgc agaattagga ttgaatattg
    43861 acgatatctc tcaaactgta ttggatgaat attttacgat gatggctgtt gtatctagtg
    43921 atgaaaagca agattttacc tatcttcgta atgaatttga agcttttggg caaactttga
    43981 atgtaaaaat caatattcag agtgcagcga ttttcgaagc tatgtataat atctaggagg
    44041 tcatcatgga tattagacaa gttactgaaa ccatcgccat gattgaggag caaaacttcg
    44101 atattagaac cattaccatg gggatttctc ttttggactg tatcgatcca aatatcaatc
    44161 gtgctgcgga gaaaatctat caaaaaatta cgacaaaggc ggctaattta gtagctgttg
    44221 gtgatgaaat tgcggctgag ttgggaattc ctatcgttaa taagcgtgta tcggtgacac
    44281 ctatttctct gattggggca gcgacagatg cgacggacta cgtggttctg gcaaaagcgc
    44341 ttgataaggc tgcgaaagag attggtgtgg actttattgg tggtttttct gccttagtac
    44401 aaaaaggtta tcaaaaggga gatgagattc tcatcaattc cattcctcgc gctttggctg
    44461 agacggataa ggtctgctcg tcagtcaata tcggctcaac caagtctggt attaatatga
    44521 cggctgtggc agatatggga cgaattatca aggaaacagc aaatctttca gatatgggag
    44581 tggccaagtt ggttgtattc gctaatgctg ttgaggacaa tccatttatg gcgggtgcct
    44641 ttcatggtgt tggggaagca gatgttatca tcaatgtcgg agtttctggt cctggtgtgg
    44701 tgaaacgtgc tttggaaaaa gttcgtggac agagctttga tgtagtagcc gaaacagtta
    44761 agaaaactgc ctttaaaatc actcgtatcg gtcaattggt tggtcaaatg gccagtgaga
    44821 gactgggtgt ggagtttggt attgtggact tgagtttggc accaacccct gcggttggag
    44881 actctgtagc acgtgtcctt gaggaaatgg ggctagaaac agttggcacg catggaacga
    44941 cggctgcctt ggccctcttg aacgaccaag ttaaaaaggg tggagtgatg gcctgcaacc
    45001 aagtcggtgg tttatctggt gcctttatcc ctgtttctga ggatgaagga atgattgctg
    45061 cagtgcaaaa tggctctctt aatttagaaa aactagaagc tatgacggct atctgttctg
    45121 ttggattgga tatgattgcc atcccagaag atacgcctgc tgaaaccatt gcggctatga
    45181 ttgcggatga agcagcaatc ggtgttatca acatgaaaac aacagctgtt cgtatcattc
    45241 ccaaaggaag agaaggcgat atgattgagt ttggtggtct attaggaact gcacctgtta
    45301 tgaaggttaa tggggcttcg tctgtcgact tcatctctcg cggtggacaa atcccagcac
    45361 caattcatag ttttaaaaat taagaaaata ggagaaattt taagttctat ttaagattag
    45421 acgtgtata
//
LOCUS       DAVKLW010000010        45314 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKLW010000010 DAVKLW010000000
VERSION     DAVKLW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.010"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..799)
                     /locus_tag="VW324_000551"
     CDS             complement(<1..799)
                     /locus_tag="VW324_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9726230.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHKENIVFIDDVNTTQ"
     gene            complement(1046..1414)
                     /gene="rplL"
                     /locus_tag="VW324_000552"
     CDS             complement(1046..1414)
                     /gene="rplL"
                     /locus_tag="VW324_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9726231.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            complement(1490..1990)
                     /locus_tag="VW324_000553"
     CDS             complement(1490..1990)
                     /locus_tag="VW324_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9726232.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     misc_feature    complement(2029..2164)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(2240..3499)
                     /locus_tag="VW324_000554"
     CDS             complement(2240..3499)
                     /locus_tag="VW324_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9726233.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     KAVKEALTEMLQSGTTTFNDMYNPIGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     gene            complement(3846..5594)
                     /locus_tag="VW324_000555"
     CDS             complement(3846..5594)
                     /locus_tag="VW324_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726234.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANSLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNLEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(5596..7320)
                     /locus_tag="VW324_000556"
     CDS             complement(5596..7320)
                     /locus_tag="VW324_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726235.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQVTSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDAISALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(7378..8316)
                     /gene="msrB"
                     /locus_tag="VW324_000557"
     CDS             complement(7378..8316)
                     /gene="msrB"
                     /locus_tag="VW324_000557"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9726236.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(8464..8535)
                     /locus_tag="VW324_000558"
                     /pseudo
     CDS             complement(8464..8535)
                     /locus_tag="VW324_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(8611..9480)
                     /locus_tag="VW324_000559"
     CDS             complement(8611..9480)
                     /locus_tag="VW324_000559"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9726237.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(9482..10768)
                     /locus_tag="VW324_000560"
     CDS             complement(9482..10768)
                     /locus_tag="VW324_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9726238.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRSLEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(10919..11656)
                     /gene="mecA"
                     /locus_tag="VW324_000561"
     CDS             complement(10919..11656)
                     /gene="mecA"
                     /locus_tag="VW324_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9726239.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(11856..12890)
                     /locus_tag="VW324_000562"
     CDS             complement(11856..12890)
                     /locus_tag="VW324_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9726240.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(12919..14352)
                     /locus_tag="VW324_000563"
     CDS             complement(12919..14352)
                     /locus_tag="VW324_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9726241.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(14368..15354)
                     /locus_tag="VW324_000564"
     CDS             complement(14368..15354)
                     /locus_tag="VW324_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9726242.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(15356..16459)
                     /locus_tag="VW324_000565"
     CDS             complement(15356..16459)
                     /locus_tag="VW324_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9726243.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(16449..17294)
                     /locus_tag="VW324_000566"
     CDS             complement(16449..17294)
                     /locus_tag="VW324_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9726244.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(17573..18847)
                     /gene="psr"
                     /locus_tag="VW324_000567"
     CDS             complement(17573..18847)
                     /gene="psr"
                     /locus_tag="VW324_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9726245.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMIFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(18844..19692)
                     /gene="pheA"
                     /locus_tag="VW324_000568"
     CDS             complement(18844..19692)
                     /gene="pheA"
                     /locus_tag="VW324_000568"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9726246.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(19689..20165)
                     /locus_tag="VW324_000569"
     CDS             complement(19689..20165)
                     /locus_tag="VW324_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9726247.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKREAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(20158..21441)
                     /gene="aroA"
                     /locus_tag="VW324_000570"
     CDS             complement(20158..21441)
                     /gene="aroA"
                     /locus_tag="VW324_000570"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9726248.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(21533..21871)
                     /locus_tag="VW324_000571"
     CDS             complement(21533..21871)
                     /locus_tag="VW324_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9726249.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(21882..22985)
                     /locus_tag="VW324_000572"
     CDS             complement(21882..22985)
                     /locus_tag="VW324_000572"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9726250.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(22995..24161)
                     /gene="aroC"
                     /locus_tag="VW324_000573"
     CDS             complement(22995..24161)
                     /gene="aroC"
                     /locus_tag="VW324_000573"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9726251.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(24171..25238)
                     /locus_tag="VW324_000574"
     CDS             complement(24171..25238)
                     /locus_tag="VW324_000574"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9726252.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            complement(25257..26111)
                     /locus_tag="VW324_000575"
     CDS             complement(25257..26111)
                     /locus_tag="VW324_000575"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9726253.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(26101..26778)
                     /locus_tag="VW324_000576"
     CDS             complement(26101..26778)
                     /locus_tag="VW324_000576"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9726254.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(26775..27938)
                     /locus_tag="VW324_000577"
     CDS             complement(26775..27938)
                     /locus_tag="VW324_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9726255.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDREVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVTFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(28623..30257)
                     /locus_tag="VW324_000578"
     CDS             complement(28623..30257)
                     /locus_tag="VW324_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726256.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLLLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKDRISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLPIISMIYLIMAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(30238..30987)
                     /locus_tag="VW324_000579"
     CDS             complement(30238..30987)
                     /locus_tag="VW324_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726257.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELLASSY
                     ELSSSDLEASLARLLSVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            31166..32620
                     /locus_tag="VW324_000580"
     CDS             31166..32620
                     /locus_tag="VW324_000580"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9726258.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(32710..35328)
                     /gene="alaS"
                     /locus_tag="VW324_000581"
     CDS             complement(32710..35328)
                     /gene="alaS"
                     /locus_tag="VW324_000581"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9726259.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAAILKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(35350..35835)
                     /locus_tag="VW324_000582"
     CDS             complement(35350..35835)
                     /locus_tag="VW324_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9726260.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(36110..36349)
                     /locus_tag="VW324_000583"
     CDS             complement(36110..36349)
                     /locus_tag="VW324_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726261.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(36610..37680)
                     /locus_tag="VW324_000584"
     CDS             complement(36610..37680)
                     /locus_tag="VW324_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9726262.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLEQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDLGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKSEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNAAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDA
                     AYAFINFMLKPENALKNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(37677..38450)
                     /locus_tag="VW324_000585"
     CDS             complement(37677..38450)
                     /locus_tag="VW324_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726263.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGNDMNSFTGFSLSH
                     FKTMFGDGCLMLIVTQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(38447..39253)
                     /locus_tag="VW324_000586"
     CDS             complement(38447..39253)
                     /locus_tag="VW324_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726264.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSAIGVILILTMFITMWVTKERRER"
     gene            complement(39234..40391)
                     /locus_tag="VW324_000587"
     CDS             complement(39234..40391)
                     /locus_tag="VW324_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726265.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEKRDEENKL"
     gene            complement(40552..41457)
                     /gene="murB"
                     /locus_tag="VW324_000588"
     CDS             complement(40552..41457)
                     /gene="murB"
                     /locus_tag="VW324_000588"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9726266.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(41569..42321)
                     /locus_tag="VW324_000589"
     CDS             complement(41569..42321)
                     /locus_tag="VW324_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9726267.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(42331..43062)
                     /gene="budA"
                     /locus_tag="VW324_000590"
     CDS             complement(42331..43062)
                     /gene="budA"
                     /locus_tag="VW324_000590"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9726268.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            43204..44475
                     /locus_tag="VW324_000591"
     CDS             43204..44475
                     /locus_tag="VW324_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9726269.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(44495..45310)
                     /locus_tag="VW324_000592"
     CDS             complement(44495..45310)
                     /locus_tag="VW324_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9726270.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
BASE COUNT        13406 a        10182 c         8555 g        13171 t
ORIGIN      
        1 tttgtgtagt attaacatca tcaataaaaa caatattttc tttgtgtaaa tttagattgc
       61 gttttacatc ttggtaaata tcctgattat tgctcttgac acaaataatg ttatatttat
      121 ttcgatccag caaagagcaa atttcaattg ctaatctagt tttccctgtt ccaggcttag
      181 cagatattaa gaagattcgt ttttcacttg atttaatggt atttataatt tcacttacat
      241 ctccaatata gtcatttgat ataggagcat tagttttact ctcatcatga attcttatga
      301 agtcattgat agaccaaact tgctctgtag attcactgat tgatagatac tcttttgcta
      361 aatattgaaa atctatcttt gtaagatcat tagcaatttc attaattcca atcaattcta
      421 gatgataagg ctcagccatt ttttctagat cttctttttg tttaattgtt atatttgaag
      481 aagtatgaca acaaataagt ctcccaactt tatcttcagc aatattttcc tctaaaattt
      541 ttttcttaac tgactgaatg tctccttcta atttagatat tacagactta tgggtgccat
      601 acataatata aacatattta ccattttcga cagcgtaaga atctgggatc cctttagttg
      661 ttttatctgt tccttcctgt gagcctaatg aaacaatatt aaaattgtat tttctactta
      721 ggtacgcgtc acctagtttt tgaaacttcc ctccctctag ctctttaata tgttgctcaa
      781 ttttatttct taaactcatt ataaaaacct ttccagataa gtatttcaac ataattatat
      841 cacactagtc aaaaccataa ggtatagttt aattaacaaa ctcttgtact acgttaattt
      901 agagtagtaa accaattata caaaaattat ttatttccta aacaactata taaaaagttt
      961 gccccttttt aaacaaaaag aaaaaccgct actcctaaga atagcggttt aatcatgttt
     1021 ttaagctacg aatgtagttg ctctattatt taagagtaac tgaagctcca gcttcttcca
     1081 atttagcttt gatttcttca gcttctgcag ttgcaacgcc ttctttaaca agtgctggtg
     1141 caccgtcaac aagttcttta gcttctttaa gaccaagacc agtgatttca cgtacaactt
     1201 tgataacgcc aacttttttg tcgcctgcag atgtcaattc aacgtcgaat gaatctttag
     1261 cagcaccagc atcagctgca tcagctgcag caacagctac aggagcagct gcagttacac
     1321 caaattcttc ttcgatagct tttacaaggt cgttcaattc aaggattgaa gcttctttaa
     1381 tttcagcaat aatgttttca atgttcaatg ccattgttat ttcctccaaa taagttttaa
     1441 attttataat agtttttttc gtagctaggc tacgctgtgt agcttaagat taagccgcgt
     1501 cttctttgct ttctgcaacc gctttgactg caagagcaac gttgcgcact ggcgcttgaa
     1561 gtacagaaag gagcatagaa agaagtcctt cgcggtttgg aagagttgca agtgcaagaa
     1621 tctcttcttt agatgcgaca gcgccttcga ttgcaccacc tttaatttca agtgcttcag
     1681 cgtttttaga aaagtcgttc aagattttcg ctggtgcgat aacatcttca ttagaaaatg
     1741 ctactgcaga tggtccaaca aatacagatg caagatcttc aagaccagct ttttcagctg
     1801 cacgacgcaa gattgagttt ttaataactt tatactcaac ttcgcttcca cgaagctcac
     1861 gacgaagaac tgtatcttgc tcaactgtca aaccacgagc gtctacaacg acgatagatg
     1921 cagcagcttt cattttttca gctactacgt caactagttc cgctttttta gcaataattg
     1981 cttcactcat tagtgtgttc acctccgtaa ttattttgct tggggaattt ttcaaaaaga
     2041 aaaacgcgcc caaacctaga cacgaaagta caatacgctt ctttttacat gatacgtttt
     2101 gtcctcggta ggatatttat gagtcgagct cccctactgt cttaggcagt ttttttaaac
     2161 cgtatataag tatagcatag tcaaaaaaag aatgcaagat ttttgcaaac tttttttaaa
     2221 atttttcgta atttttcttt taaagttcta ctgtcaggac ttgaccttgc ttaacaacct
     2281 gttctccggc gatataaaca tcatctacat cactagattt aactgcataa accaggtgag
     2341 acagcatatt ttcctgaggt tggagatgaa ttttcccttg tggttgaatg accagaaaat
     2401 ctgcttgctt gccgacttcc agacttccta tctgattttc cattccaagg accttagccc
     2461 cttcgattgt cagtaccttg agagctgttt cgattggaaa ctggctggca tccccacttt
     2521 tcatcttctg aagaagagct gcagtccttc cttcctcaaa catatctaga ttattattgg
     2581 aagcaaccga gtcagtcgca attccgactg ctactcccgc tttttggagc tggataattg
     2641 gagcaattcc tgatgccagt ttgaggttac tgataggatt gtgggcgata gccacttgag
     2701 aagatgccaa gcgttcaatt tctctctcgt ttaattcgac cccgtgagca aatacggacg
     2761 gatgatctaa ataacccagt tcttcaagaa aagcaagggg gcgtttgccg tatcgtttga
     2821 ggataattcc tgactcctcc ttggtctccg ccacatggac atggagcgga atatttagct
     2881 cttttgccat ttccaaactc gcttccagca agtctctact gcagctatac ggagaatgag
     2941 gtgctaccat aaccttgaaa tttggatttt tatattttaa gatttcatct atgatggatc
     3001 gagttctgct tatagtctca gcagttgttt ctgtctctga agaaaagaga gtcggagaaa
     3061 aataacaacg catcttggaa gttttcacca cctgataaat ttgctggata tccacaccaa
     3121 tgggattata catatcgtta aaggttgttg ttcctgactg gagcatctct gtcaaggctt
     3181 ctttgaccgc cttggtagtc atgtcgggag taaactcaga ttctgctggc cagatatagt
     3241 cattgagcca ttcatggaga ttgctgtcat ctcggatccc tctcagacct gtcattgcag
     3301 aatgggtgtg acaattgacc aaaccaggca taatccaagc tccctgatag tctataatct
     3361 gctcagcttg ttctaaaaac gctggcttat cttgaccgac atagacgatt tgagaatcct
     3421 tgactgctaa gattccatca agataaacat ggaaattttg atcacaagtc acgatattta
     3481 catgctgaaa gactttcatt atagactcct tttttaaaag acaagttgta ctctcttcaa
     3541 accacttcag attcactttg ccgtaggtgt ggttactgac ttcgtcagtt ctatctgcaa
     3601 cctcaaaaca gtgttttgag ctgacttcgt cagttctatc tgcaacctca aaacagtgtt
     3661 ttgaactgac ttcgtcagtc ttatctacaa cctcaaagct gtgctttgag caacctacgg
     3721 ctagcttcct agtttgctct ttgattttca ttgagtatta cagtgaacaa tttttctagc
     3781 tattgtaaca aaaaagtcac tcgaaggcaa cttttttcgt ccataaactt tcaaaagaga
     3841 atggcttatt cagaactaaa agccgcagct ttttgcattt ggtaatactt accctttcta
     3901 tccatgagtt cttgatggtt accatattca acaatatcac catctactaa gacaagaatt
     3961 aaatccgcat cctgaatggt tgacaaacgg tgagcaatga tgaagcttgt gcggcccttc
     4021 atgagttttg caaaggcatc ctgtaccagc acttctgtcc gtgtatcaat ggaagaagtt
     4081 gcctcgtcta agataagaat ctttggaata gccagaaaga ctcgggctat ggtcaagagc
     4141 tgagcttggc cgacagagag agattctcca gcattttcca acttggtatc gtatccctgt
     4201 ggcaactgtt gaatgaaaaa gtctgcatta gctgctttgg cagcagcaat tacttgctct
     4261 cgactggctt caagattgcc aaaggcaata ttatcatgaa tggtcccttg tgtgagccag
     4321 gtttcttgaa gcaccatgcc aaactgctgt ctcaatgatg ctcgtgtata atcataaatg
     4381 gattgcccat ccagcaagat atctcccgag ctaatgggat aaaaacgcat aaggagattg
     4441 ataagagttg attttccagc acctgtcgga ccaacgatgg ctaccttact accagctgga
     4501 atatcgatag acaagtcctt aatcaaaatt ttttcaggat ggtagccaaa agagacatgt
     4561 ttaaaggaaa tagctccctt aacttggtca ctggtcaaga cttccttacc tgtttcagcc
     4621 acttcagggc tatctaagac tccatagata cgctctacgc aagccagagc actttgcaac
     4681 tcagctagca ctgaagaaat atcattaaag ggcttggtgt attgctgaac atagttcaaa
     4741 aaagtcacta aacgaccgac ggtcaaggtt gaacccatca tgatacgata agctcctact
     4801 ccagctaaaa gggcataaat gagtgcattt acaaagcgag tcgaaggatt gaccgttgaa
     4861 gaataaaaga tggctgactg agaatagcct gagtagttgt catgagcctc acgcaatctt
     4921 tggataaatt ctgtttgagc attgaaggac tggattatag tctgctggct aagcgattct
     4981 tcaatcaact gagtctgaat tcccctcgtc tctgtttgct tctggaagag atgataggat
     5041 ctcttggcaa taaagcgtga aatcaccatg gacagtggcg tcaacagcaa gactaagaga
     5101 gtcatgagga gatgaatttg gagcatggct agaatactaa ccaaaatcat caaaacacca
     5161 atgaaaaatt ggttaaaaat catggtcaag ccagctgcca actgttcgat gtccgtggtt
     5221 acacgactaa ccatctctcc actaccttgc ctatctacaa aggcaatcgg taaacgatgg
     5281 agcttatgga tgattcgctc ccgtaaatct ctggtataag agaagattag acgattatag
     5341 aggagagaat tggcccattg taccagagta tttcctatta ccaccaagag catctggaga
     5401 aaaatctgcc aaaaaactgg tgatgaacca gccactagga cttggtcaat gacctgccca
     5461 atcagaatag gtaggtaaat tgataagcca acttgggcaa tagttcctag aaaggctagg
     5521 aaaaggagga agggatggtt tgctaaatct acgactaaac gtttgagcgt ctggtttaca
     5581 gtttgtcgtt tcattctagt cctcctttcc atgttgggat gcattgattt cacgatagac
     5641 ttgactggat ttcatcaagt catcgtgctt gccaacagct agcaactcac ctttttccaa
     5701 gaggagaatc tggtccgcca tttgtaaagt cgaggttcgt tgagagatca aaattaagct
     5761 catgtttgga aaattttctc taatagcttt caagagcttg gactctgtaa tggtatccag
     5821 tgccgagatt gcatcatcta ggatgagaaa cggagcttgg cgcaagactg ctcgggcgat
     5881 agacaacctt tgtttttgtc cacctgagaa atttcgcccc cctgcctcaa ctagggcatc
     5941 caagagtcct tccttttcac tgacaaaatc cttagcttgc gcaatctcca aggcctgcca
     6001 gagtttctgg tcagatactt cttgattgaa acctagagtc aagttggaac gaatggttcc
     6061 tttaaagagt tcgacctttt gaggtacata ggcaatccaa gaccgccact gctccaaatt
     6121 aagaggacta cgtccatttt gataaaggtc aatgttcccc ttgtctactg gataaagtcc
     6181 aagtaagagt tgcaccaagc ttgatttacc agaaccagtc cccccaatga taccaaggat
     6241 ttgtccttga gtcatatcaa aggaaatgtc tctcagagaa ggctgggccg catcaggata
     6301 ggtaaaggtc aattcttgga cttgtaaaac ccgatcactg gtaacttgct tttgttctaa
     6361 ttctgaatgg atatcctctg gagcctcaac aaagacttcc tcgattcgct tggctgagat
     6421 ataggactgg ttgagggaat ttatcaacat ggctagcttg accaattcca ccaaaatctg
     6481 taagaggtaa ttgataagag caatgagagc accttgactg agcactcctc cttgaattga
     6541 aatatagcct tgccagataa taacgagaag agttccattg acaatcagat aggtcagagg
     6601 tgttaataaa ctagaccaga aacctgtctt ttcttgtaat ctagcataaa cttggttaag
     6661 ggtttgaaaa atctgtaact ctcgtttttc ttgaccaaaa gcacgaataa cccgcatccc
     6721 ttgcaattgc tggcgcgttt cctgaaccag ttggtccgtt ttctttctga gactactgta
     6781 gagaggattg accaatcgag ataaccctac aatgacaatg gtcaaaatgg caaccatgac
     6841 taagaaccag aaagtcaact cagctgagat tcgataagcc ataaaaatgg caccaaaaac
     6901 gataatgggc gctcgtaaaa agagacgcag gaattgattg ataccagtct gaatctggta
     6961 ggtatccgaa gtcaagcgag tgaccaaact agaagttgtc agacggtctc tgctgtcctt
     7021 gggcaaggaa agaatatgac gataaagatc gtttgtcaat tccttagcaa aacctactgc
     7081 tgcctttgct gagtaaaatt gagctatcaa ggccactaaa acgccaatta ctgcaaagat
     7141 aaggagcagg ccaatctgca tccagagatg accttgatct ccctgaggta aagattggtc
     7201 aacaatccca gcaatcacca tgggaaccaa gagctcaaaa acagcttcta acagcttgaa
     7261 caagggggct aaaattgatt ccttgatgta gggtttgaag taagataata ggtgtttcat
     7321 aaatcccttc tattcatatt ctagaaatga agaaagtggg aagccccact ctctgtttta
     7381 tttgtttaag taaggcaata gatagccata tcctgctttt tccatctcat ccttggccac
     7441 aaagcgtaaa gaagcagaat tgatacagta acggaggccg cctaactccc gcggtccatc
     7501 tgtgaaaaca tgacccaagt gagcactgcc tgaacgagaa cgaacttcaa ttcgctccat
     7561 tccatggctc agatccttgt aataatgaat caactctttg gaaatcggac ggctaaaact
     7621 tggccaacca caacctgaag caaacttatc cttggcaaaa aagagtggct cacctgtcgt
     7681 aatatctaca taaatcccct cttcaaaggt ttggtcatag gcattggtaa atggagcctc
     7741 tgtagcagct tcttgtgtga cacgataaga ctcttcagat agactggcct tcaacacctc
     7801 ttgactaggc ttttcatagt ttgctgcatc aatcaatggc ttatcagcat cggtcacatc
     7861 gatatgacag taacctgaag gattcttcct gagataatct tggtggtagt cttcagccag
     7921 aatgtagtgg cgtaattgct ccacttctac tgcaatcttt cgacccagca tgcgttcctg
     7981 ctcctgtacc actgtgtaga tagctggcaa atctgcttca tcctgataat aaatcccagt
     8041 tcgatattgg cgaccacggt cattcccttg ttgattgata gatagaggat cgataactcg
     8101 gaaataataa agtaaaatct ctctgagtga cacttccttc tcatcgtaaa tcacttggac
     8161 cgtttctgca tggtctgttt ccttgatcaa ctggtaattg gtcgtttcga cttgaccatt
     8221 agcgtagcca acactggttt ctagcactcc agaaatgcgt gaaaaatatt cctctaggcc
     8281 ccaaaaacaa ccacctgcta gataaatttc tgccatcttt atttctcctt tatcacgttt
     8341 ttaactaata ctcttcgaaa atctcttcaa accacgtcag cgtcacctta ccgtatatat
     8401 gttactgact tcgtcagttc tatctgcaac ctcaaaacag tgtcttgagc tgacttcttc
     8461 agttctatcc acaacctcaa aacactgttt tgagcagcct gcggctagct tcctagtttg
     8521 ctctttgatt ttcattgagt ataaacttct tttcacaaaa aggatatgaa accctctggt
     8581 caagagtttc cccatcctat cttctattct ttattttgct tctacacgga caccttgggt
     8641 atcaactctc aagtcatgca gttttccttt gaaaggttgc ttttccaatt ctgccttaat
     8701 tgttggcatc ttgtcatgag aagccagaac cataactgtc ggcccagcac cagaaaggta
     8761 ggttgcatag gccccatttt ctttggtcac ttgcttaatc atcgcaaatt ctcttaccaa
     8821 gtcctgacga tagcgctcat ggaagaggtc tccctcgatt gcttgcccag cggtcaccat
     8881 gtctcctgcc aacaaggcag caaccgctac attggcgata gaacttgcag caacagcttc
     8941 cttataagac aattttttag gcaagacact acggctgtcg cgagtacgta attcatagtt
     9001 tggaatgtaa gctagaaaat cacactctgg aaagtctgct acgatagcag agacttgccc
     9061 ttcaacagaa cttgcaataa cgagattacc ataaatggct ggagccacat tgtcaggatg
     9121 cccttcaatc ttggtcgcta actgcaattt ttcatggtct gataagttga gttgacccag
     9181 ttggttggct agttcaatcc cagcaacgat aaccgagctg gaagaaccca aaccgcgcgc
     9241 caaagggaca tcactggtca ttttcaagcg tcttggttgc aagtctggta caatttgcaa
     9301 agcgattttg agcaagagat tacgctcatc atgtggaatc catttgccaa tctggtgttc
     9361 aatcagccac tcatctcgtt cttcgcagac ctcaatttga agatacttgg ttacagctac
     9421 accgaccgag tcaaaacctg gcccgatatt ggcactggtt gcaggtacaa taatcttcat
     9481 cttattctcc tagcaccttg aaggtattca agaggtcgaa ttctgaaacc ttcttcaatt
     9541 cagctgagac attttcaagc tgggctttat taatcttgtg tgtgatgata acgacacgcg
     9601 ccttgtcacc ctctttgcca tcttgaagga tttgcttaaa ggaaatatct tgagcattga
     9661 agatttcagc caacttcaag acctgacctt ttgagtctgg agccaagatt gagaaatagt
     9721 agtttgcttt gacatcttca ggatttgcca agaccaagtc acggctatat tcgttgaagt
     9781 ctttgccaat ggtaccatca ttcaaacgac gaacgatacg gacaatatca gctacaacac
     9841 ttgttgcagt tggtttttga cccgcacctg gtccgtagta catagactca ccaataccga
     9901 tagattctac aaagacagcg ttcattacgc cattcacact agcaagtggg tgcgctttag
     9961 gtaggaaggt tggagtcact tctgcagcaa tacctgaaga agtttcctca atagaaccaa
    10021 ccaatttcac tacgtaacca agctcttgag ctacagctac gtcttctggt gtgatattgc
    10081 ggattccctt gtgggctaca tcatcaaagg caatcttcat gccaaaggca aattggctca
    10141 aaataaccat cttgtaggct gcatcaatcc catctacgtc attcgtcgga tcgctttctg
    10201 caaatcctag acgttgtgct tccgcaagag catcatcgta agaccagcct tcttccacca
    10261 tcttggtcac catgaagttg gaagttccgt tgactactcc aagcacgcgc gtaattttat
    10321 cagaagccaa ggaatttgct aaagtacgaa gaattggaat cccaccagca actgctgctt
    10381 cgtagtaaag tgctaccttg ttagcttgag cgatttctag caattctgcg ccatggacag
    10441 ctaaaaggtc cttgttagca gtaacaacgt gttttccagc ttccaaggaa cgagtgataa
    10501 aggttttagc aggctcaata cgccccatca attccactac gatagtaata tcctggtctg
    10561 ataaaatatc atccacattg gttacaaagt taaagtcatt ccctgctgca agcaagcgat
    10621 ttttttcatc ttcatccttg accaatacct tagcaacttt gatatctgaa tgtgctgatt
    10681 gattgatttt tcctccattt tcctttagga ggaaaggcac accacttgca acggtaccaa
    10741 atcctagtaa agcaatttta actgtcatct ttatctcctc gaaattttct aatatagcca
    10801 ttataacaga attttgtgaa aattcctatt atagtaaatc actatttcag tataaaaaga
    10861 aaaaacgaat cagacgattc gctcttctta aaatctgaaa atagctttcc agaaaggatt
    10921 agccgatttt ttgcagattg agcactgcat cgtgactcat caagacttga ccatactctt
    10981 gtaagactga gcgactgata tcactatcgt ctgcaaactc gcgcatacgg gccaacagcc
    11041 aagctggata tgggcttgga tgattttcaa tatccactaa aatggtcaaa taatagcgct
    11101 cgttcatttt gtagagttca gaagtttcca tttcaaaagt cactgtcttg gcaaaagcta
    11161 ccaagtcagc caacttagca aaagaaagga tgtagtagat gtaaggttct ttcttactct
    11221 cagcttcttg ttcagcttgc tcttgctctt cttccttggc ttcaacttgc tcaagagatt
    11281 gaatggcttc gatatcatcc ttggttttgt ctgcgatgct tttttccagg gttttgataa
    11341 attcatctgg agacatttga gccaattctt ccatatctgg caaatccgat aagtcttcaa
    11401 aatctagatt ttggtcaatc tttgacttgg tcacaaagac atctacctta tcaggttttg
    11461 gagtcacacg gaagctcaac atgcctgtat ccagaaagct atcaggcatc tctagctcat
    11521 ccaagatagc ataaaagaac tcttctgttt tttcttgagg aacgagaaag tcagcaatct
    11581 ccattccacg atccatcaaa tcctctaaag acatcgtgat ttttaaagtt gtatcactaa
    11641 tttgtttcat tttcattgct agtaacctca tactttcagt tctatctatt atactagatt
    11701 tttacgattt tatcaaaaga aggctcctct atacggatag attttcccta gggtctttct
    11761 ataggagact ccaaaagaaa atttctgcag gcagatagaa aaagccttca aaatcggctc
    11821 ttagccgatt ttgaagacct tatacatcag aatacttata atttaaaggt tgctacaccg
    11881 aggatagaac gatttaagtt tctgagaatt tgaagacttt gctcaaattt cttataacga
    11941 gtcactccgt actcttcaac aagaaggact gtatctcttt ccaaaagaga tgatacatcc
    12001 tgtaaatcta caaaatgcat tccttttaaa gcttcttgac tctgtttcaa tttatctaag
    12061 atagctttat ttgagctaac gatggtcaat tcctgtccag tatttttgta tgacaaaaca
    12121 tctgctaggt tagcaattgt tgtaatctct gttacaaaat caatttgata ctgagaaaaa
    12181 tcacctactc tattgattgt tggattaaag agataaacta acacatttcc catcacaacc
    12241 aaaatcacac aaaccactcc aataacaact aaacgaagaa tcagattttt cacatttaag
    12301 ccaagcgctg tttcaccatt tgcgttcaat tctttagagt tgatggtttc cagtttttca
    12361 attttcacat ttgcataggc atgtttaaat ttctcaatca acccatcaat ttttttctct
    12421 aacaagtcat tggcatcttt acttgatgtc aaaattttca caccaacccc tgcatcgtca
    12481 atcatatagt agacggtcaa ttttttccac caatagtcat tcgttgaatt tttcaaggtt
    12541 gtttctgtcg tgtctaattc actggcaatt tttttcaact cactgggttc tacatcattg
    12601 aaaagataag ctccattcaa attaccatca atcaatttcc cataaaaatc actataacca
    12661 ccaatttgat gattcaaaat cgttttggcc gactcttttg gaggagtgat tttatagata
    12721 agataagttg aataacttgt tgtatctttg acagtgtttt tattcctaac tgctttaatt
    12781 gtaaatggta cagcaatgag agcaaataaa gcgatgagag ctaaaatatt tgcttttcgc
    12841 tttttataaa gatttgcaaa caaatcagct actgaataat gttcaaacat gatttttttc
    12901 tcctttgttt agtagatact agttttcctt tgtaagcatt tttgctacaa atataatcac
    12961 aagaacaatt ccccagaatt gcattgtaaa taaattgaag aaactttctg aaaagctgct
    13021 tcttggcata aagaatagat tattcaagat gagtagggat aaagcaaata ggattgtcct
    13081 tgagcgatag gctacttgca gcatggctat aaataatacg ccgagtaaga aactaagcag
    13141 aaagactcca atcataccat agtcggtata caactccatg atataactac ttccgatacc
    13201 atgccctttc aagtattcct tgttcaagac aagataggat agattgtggg cataactatt
    13261 actatcaata gctagttcca cactattggt tgtatgttca aaggcttttc ctccgaaaat
    13321 ggctcccaaa ctcccccttg caaaataatc aagaacagga ccaaaagtaa aattacggaa
    13381 atctcggtaa gggaggctac tgttaaatag aaaacctcga gccagaacac caaaactagt
    13441 cccttgttta tagataaagt caagtaagat atcccagaaa cctgtatggg aaacttggac
    13501 attatcccgt acataattga gtactcccat cgctaacatg agaataggag aacctacaaa
    13561 aatcgctaac ttttctttaa acccaatcca ttttcctttt tcagtttgct cccgcataaa
    13621 gtaataaaca aaagcaaata aaatacttaa aataaaggga tttcgtgtcc caattgccaa
    13681 atgaatagta ttagctgcaa taaaggagac aagcactgct gtggcctgca atttctttgg
    13741 cttggttgcc agatacatac acattgcata gaccgtaaag gtagacaaaa tgtaggtaaa
    13801 ataaggcagt ttactttcaa aatttgcata gtaggcatag taggaagtct gcaaacgata
    13861 caagagccgt tcaaataacc gaatgaaata gaaaggataa gttagaagaa aaactcctag
    13921 tgatacaaag cgtaaccgct tgatataaac ctcttttaga gaatttccta tatttgctac
    13981 ttttattttc ttcctagcta tgaagtaacg agccagaatg cctcctgtgg tcaagcccag
    14041 aatcgaaatc atgacaacta taaaggcaaa acgataggct attggatgat aggtatccaa
    14101 agcaccatcc ctaaaataat caatggtcgg tcttgatacc agaaatacaa aaatggttaa
    14161 atagaaaata aaatggatta agtaatactt gatatcattc caacaagcaa ttaagctact
    14221 aaccaacaag aacaataaag tagaaagtaa gctaacatta ttattattaa acagatacac
    14281 aattccactt actagcgtca aggcataact gactatggtc aaactaaata ataatcgttt
    14341 cccatcaatc acttggtcac ccccgttcta atgtaatttt ttagattttt caatattttt
    14401 cagtaataag aatcgatata aggaaatatt tatgaatagg gccaaagcac taattcttct
    14461 ccccttacgg aaaattggat tcctagaaat agcaaaggca tggcctttta aaaaacgatg
    14521 aatctgagaa taggcttcaa actgtttata ctgatcatct agcaacatct tatccagaat
    14581 aaagaagtgg gcataggcca atctgaaaaa agcgacctct ttcaagtcag gatagttttt
    14641 cacaacttca ttataaaact tttggtagat atcaatatag gctaaatcct tctctgcata
    14701 gggtttggtc gtaatactat cccctctatg gaaatagtaa taatagggtt tagtattaac
    14761 cacatacttc ttggccaact tgattaaatc aaaatggtaa taggcatctt cgtaaatcaa
    14821 ccccttagga aaggataggt cagttgcaat ctgtctcttg attagcttat tgcaaatcgt
    14881 cccaggtatt ttttcaccta tgaggtattc ctttagaaat gtttgagaat cacagacaaa
    14941 atagtcatcc tgattggctg actgtgggct ttcatcatta gcatagacat tcatgacacc
    15001 acagctcgaa acatccgcat cttcttgaac taattgctca tataagctct gaatcatttc
    15061 tggatggata taatcatctg agtcaataaa aatcagataa tccccgtgag cctgcttcat
    15121 cccatcattt cgtgcttgcg acaatccttc gttcttttta tgaagcactg acaccctgtc
    15181 atcttgttca gcgattgaat cacacaagcg accactttca tctgttgcac catcatcaac
    15241 aagaataatt tccagatttt gataggtctg cttctgaatg gaagctatcg atttttctag
    15301 gtactgcgcc acattataga ctggcacaat cacactaatt aatgcagttt ccatgctact
    15361 cctctaatag tttttctact tgttcgattt gttttgtaat tgtaaattgt tgaatgaatt
    15421 ggctagcctc atcgacatca aagtttgagg cagaagtcat gtaattagta atcgcctgag
    15481 ctgcctcttg attgctctca atgatttgtc caaatcgtcc ttcttgggat aattcctcag
    15541 cccctccaac gtccgtagag ataaaaggga gtcccagact caaggcctcc acatacactc
    15601 caggaaaacc ttcttgttta gacatagaca aaagaacttt cgtctgagat agatactgat
    15661 aaggattttt ttgataacca aggaaatgta catagtcctc aatcccatac tctttgactc
    15721 gttttttcag ttcctcttcc atatcaccag ccccgataaa atagagatga tagttttttc
    15781 cctcttggtg taataatcgt atcacttcca ctacacggtc agaaccctta ttttcctcaa
    15841 tccgtccgat agtacagata ctttgaggag caatctcgat atcgatcttc tcttgagatt
    15901 tttctagaat agtctgaaaa tcatatccat tgtagattgt ctgtaattta gaagtataat
    15961 ctggataaac ttccttgata gaattgctgg tcttttttga aatccctaca attgtattcg
    16021 cagcatccaa ctggcttcta tgtgattctc ttttagagct atccttaaga agttcttcaa
    16081 tacttccatg aatccaagat atcttcttga cttctcttct tttagagaac aacagtggtg
    16141 gattcataat ggtaaaagaa acttcaacat cataatcatc ttttacaagc aaacgacgag
    16201 ccagtcttgg aaaataaatt ctcattctcc acaaaaaagc tcgtaaccat ctggtttggc
    16261 gataatcttg aagggatttt aaaatgcgta catgctttgg aacagattca tatcccttgt
    16321 caaagtgctc catttcaaga atatcaatat catacttttc tagatccaga tttgaaacaa
    16381 tggttgatag aatcttctct gcaccacctc caagagaaaa agaccacata aaaaataaga
    16441 tttttttctt agccaccata ttctcccttg tattctgtat aagacttatc catatcagcg
    16501 atgacagcat catgatgcgg tacctgcttg tctgctggtg gaggcgtcat ataatcccca
    16561 aaagcagttc tgagatagac atcatagccg attggaatag gcatctctgt tccttcaaat
    16621 ggcaagaaaa gattgtcttc aaaagatgtg attgggtact tgtttctcat gtagccagga
    16681 cctgagcata attctgtaat gccatcacaa tcagccaaat catacttagt catttctttc
    16741 tcagcttttt tccagatgcg ataacggaga gattttggag tcaaacccag taaaatgcga
    16801 cttccccatt tcatgagagc accatgcttt tctggaatag tttgcgcaca aaagagtgaa
    16861 taaatcaagg cccaacgaac ctgttttttc cgctcagctg gatctttcgg ataataatcc
    16921 aaaggcaaaa catccaaggc cagaccatgt ggcaaatcca aatcctgctg ataaggcttg
    16981 atacaggtgg tttccttgtc acgaatggta ataaaaagat tacgatcaac aaaatccttg
    17041 taactctttg acaagaaata acgttcatct gcataacgag gccataattc tgctaatttc
    17101 tcataatctt tacgaggcat aaaaaagtct aggtcgtcgt cccaaggaat aaatcccttg
    17161 tttcgaaggg taccaatagc gcctccgcca cagagataac agagcaaatc atgttcttta
    17221 caaaaggcca caaaatattc agccatctcc agactacgag cctgaattgc ttttaaatca
    17281 gtcatattgt tcattattct ttctatcgta tcgtttcatt ataccacaaa caaggggtga
    17341 aaatctattg cagactgtaa aaaatcaaag cctgactgct atccaaatag ctatcaaact
    17401 ttgatttttc tgtcttatac tcttcgaaaa tctcttcaaa ccacgtcagc ttcaccttgc
    17461 cgtaggtata ggtaactgac ttcgtcagtc ttatctacaa cctcaaaact gtgtttttag
    17521 cagcctgcgg ctagcttcct agtttgcact ttaattttca ttgagtatta tcttatctca
    17581 agcccatttg agcgagcttg gtttgatatt tgttttgatc aaccagcaag cccaagcctc
    17641 cataaacatc ataggcatct acccagtcac ccagttctgg aatcgtcaat ttttcaatac
    17701 cattttttgc tccatccaaa acagataaac cgtttgttag gaggaaagta tagggtacgt
    17761 tggttgaggt catagcaaaa acctttccaa gagcttcaga accagtgaaa agtttagtgg
    17821 gatctttaat ttgctctaaa attgctgtta aaacttgttg ctgtcttttt gtacggccgt
    17881 aatccgcctc atcatcatca cggaaacgag cataattgag cagggtcgag ccattcatct
    17941 gctgttttcc gactttaatg gtttgggttg gagactcagt ctcggtagca tataaatcat
    18001 ctccgactgt agcttctgtt aggggacgcc cattcaatgt tgaaaattga gcatcaatcg
    18061 tcaccccatc agggaaaagt gtgtcaatcg ctgtggcaaa ggcctggaaa tcaaccaagg
    18121 cgtagtactt aatgtccaag tcaaaattat ctttcaagac ttggcgaacc atttctgccc
    18181 ctttttgccc ctcttgttct cctaactcgt aggctacgtt taacttgtta tctgtctgtt
    18241 ttctaccgtt aatcacttga ctataaccat ctatatagac caaattatca cgcatgaaac
    18301 tgactagctt cattttctta tctgagcccc cgacatttaa taccataata gagtcagttc
    18361 gtgtctcaac actgttctgg ccgattcgac catcagtacc catgattaaa atattaactc
    18421 catctctagt gtcctgacca ttaaagactt ctacttgagc tgcccgggca tcagcagttt
    18481 tctttgcgct agcatcttgg taaccacgca aaaacatgaa tatcatggcc aaagccacac
    18541 agaccaaaag tgaaaaaatc accataaaaa ttcgtttaag acggagcttc cgtcttttct
    18601 tttttggagg gaaagagagt gcttgtgatt tggattgtga gcgactccgg ttcgcatagc
    18661 ttggtaagtc aacctgctct tctctttctt gttccaagct agagctacta tttcccctag
    18721 caagagttag cttttcttgc aaatagacaa actcattttt ttctctctca ttgagatagt
    18781 gaatattttt tagcaaataa tcataacgca actgctcatg atgacttaag ggattttctt
    18841 tactcatctt ctctcctttc catggtctga tattggataa ataggatagg cacccagaat
    18901 tttatactgg attccaatcg cttctaattc tttttgggca aagtggacca agtccttatc
    18961 ggtataatcc acatcgataa tgaaaaagta ttcacccagt gctgtcttga gtggacgact
    19021 ttcaattttt gtcaagtcaa ttcctcgcca agcaaaggtc gacagggcct tataaagtgc
    19081 acctggaagg ttgtcaggta atgtcaaggc caaactcatc ttttcagttt gtgcttgcaa
    19141 gggaatacta ggcttttcag ctcctagaac ccagaaacgg gtgaaattgg cttccatttc
    19201 ctgaatatcc tcggcaatca gttccaatcc atattcttca gcagaacttc taggtgcaat
    19261 tgctgcaaag ggctgatctg gatgttcgga aataaaacgg gccgcataag ctgtactagc
    19321 tgttacctcg atttgagcct ctggatattg ttcatcgatg aatttctttc cttgagccaa
    19381 ggcctgtgga tgtgaaaaaa tcttttcaat cttagtatgg cctggaacca ccatcaactg
    19441 ctgatgaata ggctgaacga tttctgctac tgcttggatg tgagcctgat gaaaaagata
    19501 gtccaaggtt tcatgaacac taccctcaat agaattttca actggcacca cagaatagtc
    19561 cactaatcct tgctcataag ccttgatgac atctgtaatg ttggcaaaag cctgcaattc
    19621 ctcatgagga aaagctgtct gcacaacgtg gtgtgaaaat gatcccttgg gacctagata
    19681 agcaattttc atcttagttc ctctataatt tcctctgggc ttagcttggt cacatccaaa
    19741 acccgactag ccacttcctc ataccaagcc tgtctttctt gaaaaatagc tactagttct
    19801 tccttgctat tatttagaaa aagcggtcgc tgattgtcct tatcagctac gatacgttgg
    19861 tagagggttt caaaatcagc tttcaggtag atgttatctg tattagtctt gagtaagtca
    19921 cgatttctct gagaaataac cactcctcct ccagttgaca cgacttggtc tgtttgtagt
    19981 aaatcagcta ggacttctga ttctacctga cgaaaggctg cttctctctt ttcagcgaaa
    20041 aaattcgcaa tggacatacc taggcgcttc tcaatcagag catccatatc aaggtaatta
    20101 gggtccaagc ctcttgcaat agtcgatttt ccagccccca taaaccctaa taacacctta
    20161 gccatgaatc aagctctcca aatcatcaaa gaaactagga tagctggtat tgatggcttc
    20221 tgcacggtca agctccacct ctccatctgc aaccaatagg gctgcgatag ctgtcatcat
    20281 gccgatacgg tggtcaccaa acgtattgac tctagcaccg tgaagagctg attttccttt
    20341 gataatcatc ccatctgccg taggagtaat atctgctccc atactattta aggcgtctgc
    20401 cacaacctga atacggtctg tttccttgac cttgagctcc tcagcatcct tgataactgt
    20461 tacaccttgg gcttgggtcg caagtagggc aataataggc aattcatcaa tcaaacgtgg
    20521 aatcaaagcg ccacaaatct ctgttccttt caagtcagaa gactcaacaa tcaaggttgc
    20581 agatttagcg actggatcga tttcagttat ttccaatttc ccacccatgg cacgaatgac
    20641 atcaataata ccggtgcgag tttcgttgat ccccacattc tgcagcacta gacgagaatt
    20701 tggagcaatc aaacctgcga ctaaccaaaa ggctgcactg gaaatatctc ctggtacgac
    20761 caccttctgt cctgtcaatt tttgtggccc ttggactgtg attttcttac catccacact
    20821 taaatgacca ccaaattgtt gcaacatatc ttcagtatga ttacgggtgt actctttttc
    20881 gataataact gactccccct tagcttgtaa ggctgcaaac atcaaggctg acttgacttg
    20941 ggcagaggca attggcaact cataatgaat aggtcttagg tttttcgtcc cttttaagcg
    21001 aaggggaggc aagtctcgtt cagtttgccc tgaaatgctg acgcccattt ttttcagtgg
    21061 aagggtcaca cggtccatag gacgtttgga aagactatca tctccaaaca tctctacttc
    21121 gaaatctgca ccagcaagga cacctgaaat caggcgaatc gaggtgccag aatttcccat
    21181 attaagggca ttttgtggcg cttttaagcc agccatgcct acaccttgaa tggtaataac
    21241 cccatcttta tcctcaattt caacaccaag gtcacgaaaa acctgcatgg tcgaaagaac
    21301 gtcttcacct cgcagaatat cataaacctt ggtctcaccc tcagccaaac ttccaaagat
    21361 aatggaacgg tggctgatag acttgtcacc tgggacgcgg atactaccat gtaaatggcg
    21421 aatgtttgtt tttagtttca tactggacct catacttgca atacttttac ctattttatc
    21481 ataaaaagcc agaaattcct taaaaattcc tgactttagg atcgttcttt tcttatttca
    21541 gcaattctga aactggttca aaaacaattt tttcaatgtc agaaaggtaa atggccaatt
    21601 gttgttgctt ggtaaagaat tctgacaaga ggctatttcc ttgaatctgt ttaccaaagc
    21661 cttccatctt agcttggaag gacgcatctg gcatttgacc tgtctgtgct agtttttgaa
    21721 tttcctcttg aaaggcaaga tattctgtaa agattttgct tgcctcagca tctgctgcaa
    21781 tcgcatcttt agctgcttta acagccttgt attctggtaa tccgcgtaga ccgcgactga
    21841 gttcgtttgc actatcgtaa atatttgaca tgttcttctc cttatttgat gacgactgta
    21901 tagtcggtat tttctgttat gagatgctca gctctttcca agtcttgagc atttttaaat
    21961 gaaatttgta ggattccgtg aatatcctca cgattttcct cgttgatgtg gatgttaacc
    22021 aaggaagttc cacgtagcag ttccaaaatc cgcaggatga catcttcttc atcaggaacg
    22081 tcaacataga ggtcgtaaga gctatccaca ccaccacgct tatggatttc catggtctgg
    22141 cgttgttcac gcgcttggtt aaaaaagttc caaatttgct cttcatctcc cttactaatg
    22201 gcctgaccaa tcacttccaa acgttccttg aaatcctcaa ttctatccag aatggtctcg
    22261 ctattggaca agagaatgga ggtccacatt cctggctcgc tttccgcaat tcgggtcata
    22321 tctcgaaaac cacctgccgc aaagcgcctt gccatctcat gctcttgagc atagaccgca
    22381 gtctgctcca tgagactaga agccaaaata tgaggaaaat ggctaatctg agaagtgaca
    22441 cgatcatgct ccttggcatc aatctcgata aaacgagcat gaagacctga aagcagatcc
    22501 ttcatttcct taagcgtgtc ctgacttgtc aggcttgaag gtgtaaagat ataataggca
    22561 ttttcaaaaa gattgacatc tgccgaagca gcccctgtct tgtgactacc agccatggga
    22621 tgggccccga caaagcgaac agacttgcca gccaaatact gctccgccgc atccacaatg
    22681 gttgacttgg tcgaaccagc atctgaaata ataacgcctt ctcgcaaatc caaattggcc
    22741 aactccttaa tgaaagcaat agtttgtttg attggcaagc tgaggataat gacatctgcc
    22801 aaaggagcaa aactagcaaa atcatccgtt gcacggtcaa tcatgccttc tttcaaggcg
    22861 atatctctcg aagcttgact acgattataa cctaaaattt cataatctgg atgatcgcgt
    22921 ttgataccaa gtgccataga ggctccaatc aacccaagac ctgcgatata gattgttttt
    22981 gccataggaa ctccttaata gttctttgta tagtctcggt gtttggctac cgcttctttt
    23041 agttcctcaa gattatctga tgagaatttt tcgaggattt cttgcgccag aaccgttgct
    23101 acaactgctt ccatgaccat tcctgcagct ggaagagcag tcggatcact tctctccacg
    23161 gttgccttgt aaggttcgtg ggtttcgata tccacactca taagaggttt ataaagagta
    23221 ggaatgggtt tcatgacccc acgaacaacg atgggttgcc cattagtcat accaccttca
    23281 aaaccaccta gattattggt acggcgagta taaccgtctt ctttagacca gagaatttca
    23341 tccataactt ggctgccttt acgataacca gcctcaaagc caagaccaaa ttccacccct
    23401 ttaaaggcat tgatagagac aacagcttga gccaatcttg catccaattt tctatcccat
    23461 tggacatagg aaccaagacc aactggaacg cctccgacga ctgtctccac aaccccaccg
    23521 atggtatcac catcacgttt gatttggtca atatagtcct tgatttcctg ttctcgttct
    23581 tggttgacaa tagaaacttc agactgggca gctctttgct taatttcagc gactgtcaga
    23641 ttttcaggaa catcgatttc cttgccacca aagaccacga catggttggc aatctccata
    23701 tccagctcag ccaagaggcg tttggctact gcaccaactg ccacccgcat ggtggtttca
    23761 cgagctgatg aacgctccaa agaatttcgc aaatcatcaa aacggtactt aatcccccca
    23821 accaaatcgg catgacctgg gcgaggatga gtaattttcc gcttgctttt aaggcggtct
    23881 tcaatgtcct ccgcagacat gatgtccagc catttctggt ggtccttatt gatgacatcc
    23941 atagtaatag gcgcccctgt cgtcttcccg tggcgaacgc ccgaagtaaa gacaacctgg
    24001 tcactctcaa tcttcatacg accaccacga ccgtagccac cctgacggcg tctaaggtcc
    24061 tcattgatat cctcagctgt caatggaagt ccagctggaa ttccctcaat aatagctgtt
    24121 agacgggggc cgtgtgattc tcctgcagtt aaatatctca tacactctcc ttattttacc
    24181 aagtagtctt tcatctcttc cagagaaact gggtgaatga tcgctgaacc aagctctggc
    24241 accaagacca atttcaaggt gttaccacgc gctttcttgt catgagtaag agcctgataa
    24301 agcttgtcaa cttcccaatt ttcatagtca acaggcaagc cgaatttctg acacatctct
    24361 gtgatagatt gggtaatgcc agctggcatg aggccttttt cctcagcaac cttggaaatc
    24421 tgtaccattc ccatggcaac agcctctcca tgcatgacct tgccataacc ggcagtcgct
    24481 tcgatggcat ggccaatagt gtggccaaaa ttgaggtaaa gacgaatacc attgtccaac
    24541 tcatcttcaa ccaccatctt gcgcttcacc tgacaagaat gttcaatcaa ggtctctgca
    24601 tgttccaaaa tactctcaac agaaccattc agtcccgtca agagagccca cagttctgga
    24661 tcctcaatca agccatactt gataacttca cccatccctt caatcaactc tctttttccg
    24721 agggtttcaa gaacaagtgg atcaatcaga accccatctg gttgggcaaa ggtccccacc
    24781 atatttttag caaatggtgt gttaacgcct gtctttccac cgatagaaga atcaacctga
    24841 gctgtcaaac tagtcggaat ctgaacaaag tgaatacccc gcatataggt agaggctaca
    24901 aagccagcca ggtccccaac aacgccacca ccaagagcaa cgattccatc gctacgagtc
    24961 agaccttgct tgactagaaa ttcatagact ttctgaacag tagttaaatt ctttctttct
    25021 tcaccttcta agaaatcaaa aacagctacc tgaaaaccag catcttctag gctgagcttg
    25081 accttctctg catagagaga ggctacatgg ttatctgtca caatgactac cttttgcggt
    25141 tgccagagtt ctcgcaacca ctgaccagcc tgggccatac aacctttttc aatctgaata
    25201 tcataaggat gatgaggaat atcgattctg attttcatag gagagtctcc ctttctttat
    25261 tggtattttt ctgttaaaga ctgccaaatc tcttctgtcg gcatttcctt gcctgtccac
    25321 agttgaaaag cttctgcagc ttgatagagc aacattccca gaccattgac tgctggattg
    25381 ccctgacttc tagcccattt caaaaacggt gtttcaaagg gttggtatat gatatctgca
    25441 actaagagag tttctggtaa gactatgttt tcaggaacag gagaggattg gccatccatg
    25501 cccacactgg tggcattaac tagcaaatcc gactcggcaa tccttgcttg cagttcagaa
    25561 acatattcta aagcacacaa atccacttta aagcctgtct gctcctgtaa cttgtctagg
    25621 taaggtcttg ttttttccat agaaacggaa cgaacaaaga ccgaaatctg actgacgcca
    25681 tccaaaatag cctgtgccaa gattgattta gccgcaccac ctgcacccag cagggtcatc
    25741 tttttacctg aaattgtaaa agaaggcaag cacttaaaaa atcccttgcc atctgtatta
    25801 tatccaatta aattgccatt ctcattgaca accgtattaa ccgcaccaat caagcgcgct
    25861 tcatcgctta gctcatccaa ataaggaatc acctgctcct tatagggcat ggacagattg
    25921 atgccaaaca tctggtagcg acgaatattg gccactgttt ctgccaagtc actcgcttca
    25981 atctcccaag ccacataagc accgttggta gctgtcgcct caaaggctct attgtggatg
    26041 aagggagaaa tagaatgctt aataggattg gcaacaactg cagctaaacg tgtatagcca
    26101 tcaagcttca tccaaaatct ccctgatttt tttcatgcta gctagagaaa tctgcccagg
    26161 ggcactaacc tcatccagac tggcaaaaga ccaactcgaa ccagtcacat ccgcagtgat
    26221 acgagagacc ttgcccacct tacccataga aatggtcaca tattcctgtt caggattgag
    26281 ggttttaaag cctcgtgtat agttcatcaa gtctaagaca tcctgctccg tgtgagccat
    26341 caccgcaacc ttaacaagtt ttggatttag gatcgtcaac tctgacaaga tttccatcat
    26401 gttctcaggt gtttcttgga aattatggta actcaaaaca agatttggga agtccagcat
    26461 ttcctcaaaa acatccttgt agctatagta ctcaaaatca atatagtctg gttgatagag
    26521 ttgcgcaact tccttgatta gatggatata ctcttctgga gaaaggtcga tttctccacc
    26581 ttcggagcga gttcgtagcg tgaaaaccaa ctcacggcct gcgaattttt caaaaatggc
    26641 tggagctacc tgcaaaatcg cttctttagg cagatagtcg gcacgccatt caatgatgtc
    26701 ggcatccagg tacctcgtgg catccagagc ctgagcctcc tctaaacttc ttggcattac
    26761 tgaaacgatt aatttcattt actaaccttc atactaatca ccttgaggta attactactt
    26821 tcatcttttt tattataggc aaaatctgct ggaagaccat atttgtttaa aatctggtaa
    26881 cttcttcctg caaaaccttt atcaatttgt tctgtaaatt tctgacggga aacattggca
    26941 gcattggtac tggcaatgat aatccctccc ggatttaaaa tctcaagact ctgggaaatc
    27001 aacttgtgat aatccttggc cacagagaaa gtttgttttt tattccgagc aaagctaggc
    27061 ggatctagga caatcacatc gtaggtcaag tctttgcgtt tggcatattt gaaatactca
    27121 aagacatcca tgactataaa acgatgctcg tctgtgctga gcccatttgc ctgaaaatgc
    27181 gcttgagaca attctcgtga acgtttggct agatcaacag aagttgtctg gctagctcct
    27241 cccatggccg cagctactga aaaagccgct gtgtaggaaa acatattgag caaggattta
    27301 cccatagcca agccgtcaac taaactaccg cgaacctcat gctggtctag gaaaatccct
    27361 gtcatcaagc catcattcat aaagacttga tacaggacac cattttctaa aacagtgaaa
    27421 aagtcaggtg cttcttgacc ataaacatgg gcagattcat agtcaaaacc cttaaagcgg
    27481 attttctcat aagcccctaa aacctcaggg aaaacctgtc taaaggcttc tgatatagtc
    27541 tgacgaatct gataaacata agagttatac caagaaaaga cggcgtagtc gccataaagg
    27601 tccactgtca gacccccaaa gccatctccc tcttgattaa agagacgaaa ggcagttgtc
    27661 aaatcatctt gatagtaggc gtttctcttt tctttggctt ttctaaacaa cgtttcaaag
    27721 aaagcttgat tgaaggtcac cttgtctttg ctgataaacc agcccaagcc cttgttttgc
    27781 tgagaaaggt aggcagtccc aagaaagttt ccttcctgac cctgcacctc tacttcccga
    27841 tccttaagat tgacattctc aagatcactg gcttctagta aaactagccc cttagcaagc
    27901 ttcttttcaa cccttttgct gactcttatt ctattcataa ctaccattat atcaaacttt
    27961 tagacaattc tcaaaaaaga aactaccctt gcttttttac tcttctttta aaaaatggta
    28021 tactagactt cctgcaaaac taggaaataa atgtgtaaga atcacaataa aaaatgctct
    28081 tccgtcttgg aggagcattt ctttttatca acgaaaatca aatagcaaac tatgaaacta
    28141 gcctcaggtt aaccgtgaga ttataggtag agaggttgta tcagcaatat gtgtctgtca
    28201 aatttagtga caaaggtagt agaagaaaga tgaagaaata aatcagcttc agtagatatc
    28261 tggaaaattt gattttatag aaaagccttt tgttacaaac tcaatatact atcaataaat
    28321 aatattatag aagcaacaat aattaaaatt tcacctattt gcatcattct atttcgaact
    28381 ctaaatatat gttctatcaa aaatacttgg atcacacaca ttataggaat taacgttttt
    28441 gaaattgaaa aatatcccaa taaataaact ataaacaaca ataatagaac tatattatat
    28501 ttcttattca aaacattcct ccctatattt ttgattacca atcttaatca tttacaacta
    28561 cattctaaca aactataaaa gcgtttgtcg aattgaattt atcaagcaag cgaccaacca
    28621 gctcatcttt tttctatttc tgccaatatg cgtgacaggt aataatgata gccaaaaata
    28681 gcaagagcaa gcaggaggat aagagctcct actcccaagc tgatggcaag gatagtggag
    28741 agagactgaa ccaagaatat gctcccaatt acaagggcca tcaggattgc actataaata
    28801 aacaataaaa ctatggcgac tatgccattt gaacgattca ccaggtccgt aatgctactc
    28861 caattggttg acagattttt aacgtcctta aagtaatggt ggcaagaaag gatgacactg
    28921 gcaaggatcc atgccataat aaggtaaatc atcgaaatga tgggcaagcc tagatataga
    28981 gaaagaccaa gtaaagtcag aattggtaaa aaggactgga cggcatatat aatccaaaat
    29041 ttcactttca cataacgagc aaagtcaaag ggtaaactct taagaaaatc aacattttcc
    29101 ctctccaagg acaaggcaat tgaatgcagg ctggtgatat tgttattgac aactgctata
    29161 aacactccta caaaaaacaa gggtaaccag tatggaggat gaatgtctgg aactatctga
    29221 gaatctcgga ttttggaaat cagactgatc atcatgagat aaggaaggaa agcacttgta
    29281 aaaagcactg taatcacgcc agtcccctgt cccaagaggg tgaggtggta gcgtaaaacc
    29341 atacggaaaa agcccttttt agtagttgaa atcctatcct tgctgcgacg ttcttttttg
    29401 accttctcct cactgttaag caggatcacg tcataaaaac gaggaaggac cttctttttg
    29461 gtcagataaa gcaggaagag agttagtcct atccaagcga gcagacccac taaggcttct
    29521 gtcgaaaaag gctccactgc tattttgtaa aagatatgaa gaggataaag gagaaatgga
    29581 atgtctctaa ctttgtcaac aatacttcca aaagtcgact gaagaaagaa gataaatatt
    29641 aaaggtatga gaactcctat cccaatcatc acattcgaaa aaatagactg atactttctg
    29701 aagaccctag tctgagccaa gaaatgtact gccactaccg tcactaaagt aacagagaca
    29761 aataataagg tcaaggacag tagcatcaaa agcaaaccca accacagaga aggtgctagc
    29821 ctaatgtaga ggaccagaaa ataagctagg attggtacaa ttccagttag agctggcaag
    29881 aggacagaca gacctttggc cattataatc tctgattctt taaaggcata gggcctatag
    29941 gaggccaggt ccttactctc ataaaagaca ttgtaaaatg cagtgaaaga agttgaaaag
    30001 gccagcacca gtaaaatagc aaccatcgta ctaaaaaaac ttggcatttc ctcaaaagga
    30061 aaacgaaagg ctatatttat aaacatgaaa agcatcaaga gactagaaaa aatgtaagaa
    30121 cttaggactc tagcggaaac atttactttt ttcccaggat tcttagcctg cttctttcgt
    30181 agattagcca gattagcttc ttgagatgaa tagaggatat tgatatcaac taatttttta
    30241 atgaccttga gacgcatctg aaacctcctc ttttctacca gcaaggctaa ggtagatact
    30301 ttccaaagac tggtctgggt tgtcttttct caagtcctct acactaccac aataaatcaa
    30361 atgccccttt ttcaaaatgg caatccgatc acagacttgc tctgccacct ctaggacatg
    30421 agttgaaaac aagactgtct tccctttttg tgcatgttcc ttcatcatct gtttcaaatc
    30481 aaaggcagcc tggggatcca aaccagtcaa gggttcatcc aagacccaaa tatcgggatc
    30541 agacaagagc gccccgatga caaagacttt ctgacgcatt ccgtgagaaa gagtttcaat
    30601 aacctgatag cgattttcag caaaatcaaa aacgctcaat agcctagcta gactagcctc
    30661 caagtcagag ctactcagct cataggacga ggcaagtaat tcccaaaatt cattggctgt
    30721 taatcgtaaa aataagtcag gcgagtctgc tacgtagcca atctttcgtt tgatcgccaa
    30781 gcgattttcc gataactcct gaccgtctac caaaatacga ccactgctgg gtgaaatgat
    30841 actgactagg gattttatgg tggtcgattt tccagcccca ttatgaccaa tcaagcccat
    30901 aatctcccca ttttcaatct gcaaattgag attgctcaat gcctctttat caccatacaa
    30961 cttactaaca ttttgaaatt caatcatggg acaactccta tctttatctg tattatacat
    31021 cgctatacta gcactttgat acaaaaaaat caatacttta ttttaagtag ttaaaatagt
    31081 ttccttctat cttacgcaaa cgtttgcgcc gagagatact ttatgataga ataaagaaca
    31141 agattgacaa gtaagaggaa acatcatgca aaatcaaaca ctcatgcaat actttgaatg
    31201 gtatctgccc cacgacggtc aacactggac gcgtctggct gaaaatgctc cacacctagc
    31261 tcatctgggg atcagtcacg tctggatgcc accagccttc aaggcaacca acgaaaaaga
    31321 tgtcggctat ggggtctatg acttatttga cttaggagag ttcaaccaaa aagggactgt
    31381 ccgcaccaag tatggtttca aagaagacta tcttcaagcc attcaagccc ttaaagaaca
    31441 gggaattcaa cctatggccg atgtagttct caaccacaag gctgctgccg ataacaggga
    31501 agcctttcag gttatcgaag ttgatcctgt agaccgtaca gttgaacttg gagaaccctt
    31561 caccatcaat ggctggacta gttttacctt cgatggtcgc caagatacct ataatggctt
    31621 ccactggcat tggtaccact tcaccggtac agactacgat gccaaacgca gtaaatctgg
    31681 gatttatctg atccaagggg acaacaaggg ctgggctaac gaggaattgg tcgataacga
    31741 aaacggaaac tacgactacc tcatgtatgc cgacctagac tttaaacatc ctgaagtcat
    31801 ccaaaacatc tatgactggg ctgattggtt catggaaacg actggtgtag ctggtttccg
    31861 tttggatgcc gttaagcata ttgactcttt ctttatgcgc aacttcatcc gcgatatgaa
    31921 ggaaaaatac ggtgacgatt tctatgtttt tggtgaattt tggaacccag acaaggaagc
    31981 caatctggac tatctcgaaa aaacggaaga acactttgac cttgtcgatg ttcgtctcca
    32041 ccagaatctc tttgaagcca gtcaagctgg cgcaaactat gaccttcgtg gcattttcac
    32101 agatagcctg gttgaactca agcctgacaa ggctgtgact tttgtcgaca accacgatac
    32161 ccaacgagga caagcccttg agtctaccgt tgaagaatgg ttcaagccag cagcctatgc
    32221 cctcattttg ttacgccaag acggccttcc atgtgtcttt tacggagact actatgggat
    32281 ttcaggccag tatgctcaag aagatttcaa agaaatcctt gaccgcctcc tagccatccg
    32341 aaaagacttg gcctatggag aacaaaatga ctactttgac catgctaact gtatcggttg
    32401 ggtacgttca ggtgctgaaa atcaatcccc aatcgcagtc cttatctcaa atgaccaaga
    32461 aaacagcaag tcaatgtttg tcggtcaaga atggactaat caaacctttg tagatttact
    32521 tggtaaccac caaggtcaag ttacaattga tgaggaaggt tatggacaat tccctgtctc
    32581 agctagatcc gtaagtgtct gggcagtcaa taccatctaa tagctcataa taaccaagct
    32641 aggtccaagc ggatttggct tttttgtatt cacaaaaaga cctacccaaa tggatagatc
    32701 tttacttgat tacaatttac ctgctactgc atccaacaat tcttggatct tagcttggtt
    32761 gcttcctcct gccatggcca tgtctggttt accaccacca cgtccatcga tgattggtgc
    32821 taattctttg acaaggtttc ctgcatgaag gtcttttgtc ttgcttgcta caaggacatt
    32881 gactttgtca ccgatagcgg caactaggac aagaagatca gagtagtctt tttgtttcca
    32941 gttatctgca aaagtacgaa gggcaccggc atcggataca gacacttgac tagcaatgta
    33001 acgatgaccg ttgacttcct tagcatcttt gaagatatcg cctgcggctg cagctgcggc
    33061 tttttctttc aactcagcat tttctttttg aagttgacga agttgttctt gaagtccttc
    33121 taccttgtga ggtacttcct tgacttgagg tgctttcaag attgctgcga cagctttaag
    33181 agcatcctct tgttcacgat aggcttcaaa ggcttcctta ccagtcactg ccaagatacg
    33241 gcgagttcct gaaccgattc cttcttcttt gacaattttg aagagaccaa tctcagaagt
    33301 gttgccaaca tgagtaccac cacaaagttc aatagagtag tcaccgatag tcacgacacg
    33361 aacttccttg ccgtatttct caccaaagag ggccatagct cccatttctt tagcagtgtc
    33421 aatatccgtt tcaactgtct taacttcaag agcttcccag attttttcgt tgacttgctg
    33481 ttcaatcgca cgcaattctt cagcagttac agcttggaag tgggtaaagt caaagcgaag
    33541 gaattcaact tcgttaagag atcctgcctg tgttgcgtgg tttccaagga tattgtgaag
    33601 ggcagcgtga agcaaatgag tcgcagtgtg gtttttcatg acacggtgac ggcgattgct
    33661 atcaattgcc aaggtatatt cttggttcaa ggcaagcggt gcaaggactt caactgtatg
    33721 aagggcttga ccatttgggg ctttctgaac attggtcaca gtagccacaa ccttacctga
    33781 ctcatccaag atttgtccgt agtcagctac ctgtccaccc atttcagcat aaaatgacgt
    33841 ttccgcaaag ataagagagg cagttccttc tgaaacagct tctacttctg cattgtccgc
    33901 cacgatagct accaatttag aagacaattg gctagcattg tagttgaaga cactttctac
    33961 agtgatgttt tgaagagttt cattttgcat acccattgag ccacccttga cagctgaggc
    34021 acgcgcgcgt tcttgctgtt ctttcatggc tgcttcaaaa ccttcacggt ctacagtcat
    34081 accagcttct tcagcgattt cttcagtcaa ttcaactggg aacccataag tatcatagag
    34141 tttgaagaca tctgaaccag cgataacaga ttgacctttt tctttcaagt ctgctacaat
    34201 gccttgggca aagtgttgac ctgagtgaag ggtacgggca aatgattctt cttcgctctt
    34261 aacgattttc tcaataaagt cacgtttctc aagcacttct gggtagtagc tttccatgat
    34321 ttttccaaca gttggaacca atttgtaaag gaaaggttcg ttgataccca atttttgacc
    34381 atgcatagaa gcacgacgga gaagacgacg aagaacataa ccacgacctt catttcctgg
    34441 aagggcacca tcaccgatag caaatgaaag agaacgaatg tggtctgcga taaccttgaa
    34501 gctcatgttg tcgccatctt ggtcataaac cttaccagac aatttctcga cttcacggat
    34561 aatcggcatg aagaggtccg tttcaaagtt ggtcttagcc ccttggataa cagccaccaa
    34621 acgctccaaa ccagcgcccg tatcaatgtt cttatgtggc aattccttgt attcgctacg
    34681 aggaacagca gggtctgcgt taaattgtga caaaacgatg ttccagattt caatataacg
    34741 gtcgttttca atatcttctg caagcaggcg aagaccgata ttttctgggt caaaggcttc
    34801 cccacggtca aagaagattt ctgtatctgg tccagaaggt cccgcaccga tttcccagaa
    34861 gttgtcctca attggaatca agtgacttgg atccactccc acttcaatcc agcggttgta
    34921 agaatcttta tcgtctggat agtaggtcat gtaaagtttt tcagcaggga aatcaaacca
    34981 ttcagggctt gtcaaaagct cataagccca agtgatagct tcgtcacgga agtaatcccc
    35041 gatagagaag ttccccagca tttcaaacat ggtatggtga cgcgcagtct tccctacgtt
    35101 ttcgatgtcg ttggtacgga tagccttttg ggcattggta atacgtggat tttcagggat
    35161 aatggtcccg tcaaagtatt tcttaagggt tgctacccca gagttgatcc acaaaagagt
    35221 tgggtcattt acaggaacca aacttactga tggttctact gagtgagctt tggtcgccca
    35281 gaaatcaagc cacatttggc gtacttgtgc actagatagt tgtttcatat tgtctcctta
    35341 tttacttgtt taatgtgatt ggctttccag catttccaca tagtcaatcg cgacacagag
    35401 ggaaatgact aggtctgcat aagcgtcctc aagaaccgtt acggtatagg tagaggtcag
    35461 atggaagagt tccttcttaa tttccgcaat cagctgatcg cgatcatcca gcaatttgaa
    35521 attcaaatcc cagatattgc cctcgatacg aagacctaga ttatcaaact catacttatc
    35581 tcgccagaag gtcaacttct tacgaatgac aaaactcgag ccatcccgaa gctgaatctc
    35641 aaaacgagga agtaaggtca agatttcttt actaatctca ctgacttgtt caccagccgc
    35701 atcatagatg gtaaaggttt taggaatctt aaaaaatgat ccctccacct gataggcaat
    35761 ttctcccctg tcatccttga tagcgaagcg ttcgcctcca agacgaaact tttgtttgac
    35821 aagaaatgtt ttcatcaaca cctccaaaaa tcaaaagaca agctcatatc acgaagggcg
    35881 aaaaaccgcg gtaccacctt cattcaatga acttgtcatt ctcttgttct tatgcaattg
    35941 tatgattgag tagcatgact tcctagctta gatggctcgc agcaccgcca tttctctgga
    36001 ctaagacaag tgaaaatcaa ttctcaactt tcttattata acgttttttt aagcttgcgt
    36061 caactggaaa tgatctccgt tgaattagac caattcccta catctctgat tactttttca
    36121 ggatatattt tttcttactg ccatttttct ttttatccca aattttcata ttactaaaca
    36181 cagctactag aatatttcca aatataaagg tgcctatcac ccaatatatg gactcagttg
    36241 ttaggtattg tcgatccaag ccatccttta aatggaatag tatagcagtt tggttaacaa
    36301 tcataaaggt tggccagaaa ctttttttga aaaaagtaga cattttcatt atttgttgcc
    36361 gctttctgta aggttaatac tcaataaaaa tcaaaaagca aactaggaag ctagcctcaa
    36421 gctgtacttg agtacggcaa ggcaacgctg acgtggtttg aagagtatag gcttagtata
    36481 ctactaggca agcaaataag caaataaaca aataaacaac tagaatagaa aaagataggg
    36541 ctctaaaaac tgacttctat tccttaaaaa cgaaccagct tgactgattc gtcttcttac
    36601 gtttatctcc tacttccgat acattttaaa ctgtaggaag aggtcgctat atttccctgt
    36661 ccatttatgg tcaaatttct cataaatttc taggtgtttc atggtttcaa catcgggata
    36721 gaaggcctta tcttcttttg tttcctctgg taacagttcc ttagctggta ggtttggtgt
    36781 tgaatagcca acatactccg cattttttag agcattttcg ggtttcaaca taaagttgat
    36841 aaaggcatag gctgcgtctt ggtttttgac tgttttggga atgaccatat tgtcaaacca
    36901 aaggttgctg gcttcagtcg gtaccacata gcgtagattt tcgttttttt ctagcatttg
    36961 gctggcttca ccagagaagg tcacaccgat agcagcattg ttctgaatca tgtagccctt
    37021 catctcgtcc gcaacgatag ccttgatatt tggagtcagt ttgtagagtt tatccactgt
    37081 ctcttccaac tgctgcggat ccttggagtt gaggctgtag ccgagggaat tgagtcctag
    37141 tcccagcacc tcacgcgccc catcaaagag catgatagaa ttcttatact ccgacttcca
    37201 aaggtcatcc caatgctcag gcgcttcatc taccatggtt tcgttgtaga caattcctaa
    37261 ggttccccag aagtaaggga tggagaattt attacctagg tcaaaggact ggttgagaaa
    37321 ctctggtccg atattttcga ttccttcaat ttttgaataa tcaagcggaa ccaagaggtc
    37381 ttcgtccttc atcttgttaa tcatgtattc acttggaatg gcaatatcgt aggtcgttcc
    37441 accctgcttt atcttagtgt acatggcttc gttggagtca aaagtctcgt actgaacttg
    37501 gatgcctgtt tcttctgtaa attgctccaa gagttctgga tcgatatagt ctccccagtt
    37561 atagataacc aatttttgac tatctcgact attgatttta ctatctaaat gagtcgcaat
    37621 tccccacaag acaaggataa tcgctgcaat tcctgctaaa aatgaataga tttttttcat
    37681 gcttgctcct ccttctcacg agagataaag taataaccta caactaggat aatactaaag
    37741 agaaagacta gagcagacag ggcattgatt tctaaggaaa tccctttccg tgcacgagag
    37801 taaatctcga ctgacagggt tgaaaagcca ttccccgtta caaagaaggt cacggcaaaa
    37861 tcatccagcg aataggtgaa ggccatgaag taacctgcaa tgatagacgg agtcaggtaa
    37921 ggaagcatga tttccttaaa catctgaaat tgactagctc ccaagtcata ggccgcatga
    37981 atcatgtcgc cattcatttc cttgagtcga ggcaagacca tcaagaccac gataggaatg
    38041 gagaaggcca cgtgactaga tagaacggtc aaaaagccaa gtgaaaactt gagttgggta
    38101 aagagtatca agaagctagc accaatcata acgtcaggcg caaccatgag gatattattg
    38161 agtgatagaa aggcttcttg gtatttctta cgagactggt agatgtaaat ggcaccaaaa
    38221 gtcccgataa tggtcgctat caaggctgat aggaaggcca agaaaaaggt ctgagtcaca
    38281 attaacataa ggcaaccatc accaaacatg gttttaaaat ggctcaagct aaagcctgta
    38341 aagctgttca tgtcgtttcc tgcattaaag gcgtagccaa tcaagtaaaa aatcggcagg
    38401 tagaggacaa gaaagaccag tcccagataa aggttggcaa attttttcat cgttctctcc
    38461 tttccttggt cacccacatg gtgatgaaca tggtcaggat gagaatcaca ccaatggctg
    38521 aacccatacc atagttgtca ttggttagaa aattctgctc gatagccgtc cccaaggtga
    38581 taacgcggtt cccaccaatc aaacgggtca gcatgaagag actcaaactt gggataaaga
    38641 ccgactgaac cccacttcgc acaccgttca tagatagagg gaagatgaca tgacggaaag
    38701 tctcccactt agttgcacca aggtcgtaac tagcattgat gagattatta tccatatcgt
    38761 ccaagacatt gaaaatcggc aaaatcataa aggggagctc gatgtagctt gcgacaaaga
    38821 taaatgagaa atcagtaaat agcaactgct gtgaaccaat tccgataaat tccaagaatt
    38881 ggttaataga gccattctga ccaaaaatcc cgataaaggc ataagcctta agaagaaggt
    38941 tgatccaagt tggcagaata atcagcataa tccagagttg acggtgtttg agacgggtca
    39001 aaaagagggc cgtcggatag ctgataagca gtgtcacaaa ggtcacaatg cctgcataaa
    39061 gcactgagtt gaaactcatt ttaagatagg tcaagttttg tgacgcaaag taagatttgt
    39121 aattttctaa actgaactgg ccttcgatgt tgaaaaagga ttgaccgaaa atcaagacca
    39181 agggtgccaa tacaaagagc gcaatccaaa gcatgtaggg cactacaaag agtttagagc
    39241 ttgttttctt catctctttt ctcctcgatt gcattgatca aacctgcttc ttgctcttcg
    39301 atttctacgt actcctcaat acgagcatcg aactcttctt cggtttcatt gagacgcatg
    39361 atgtggatgt cttctggttc aaagtccaga ccgatttcct cacccacgat ggccttacgg
    39421 gttgagtgga tcatccattc atttccaagt tcgtcatagg cgataatttc ataatgaact
    39481 ccacggaaaa gctgggtatc gaccttaact tggagcttgc cttcttcagg aagggtaatg
    39541 cgcaagtcct ctggacgaat aacaacctca acaggttcat ttggcttcat ccccccatcg
    39601 accgcttcga agcgtttgcc gttaaactca accaagtagt cctcaatcat ggtacctggc
    39661 aagatatttg actccccgat aaaggtggca acaaagtggt tgattggttc atcataaata
    39721 tccacaggag ttccagactg gacaatctcg ccatcattca taacaaaaat ccagtcactc
    39781 atggcaagag cttcttcctg atcgtgagtg acaaagacaa aggtaatgcc caatcgttgt
    39841 tgcagttcac gcagttcgta ctgcatgtct gttcttaatt tcaagtccag cgctgacaag
    39901 ggctcatcca acaaaaccac acggggttgg ttgatgatag cacgggcgat agccacacgc
    39961 tgacgttgtc ctccagaaag tttgcggatg gaacgttttt cataaccttc caactgaacc
    40021 atcttgagaa cttccgctac gcgttgctcg atttctttct tgtcaatctt gcgcaagcga
    40081 agtggaaagg caacattttc aaacacattc atatgcggaa acaaggcata ggattggaag
    40141 accgtatgga catcacgctt gttggttgga atatcattga tacgaacacc gtctagcatg
    40201 atatctcctg tcgtcgcatc cagtaaacct gcaataatgt ttaggatagt tgatttcccc
    40261 gaaccagatg cacctagaag ggtgtagaat ttcccttctt ccaactcaaa gttgatgtct
    40321 ttgagaacct tggtgttgct gtcttcaaaa actttagaga cgtttttgaa ttcgataatt
    40381 ggttttttca attggtataa attccttctt tttcatagat taacagatcg gggctctgtc
    40441 aggtcgccac tacctcttgc agggagtaaa accacctgca tacatcttcg ctaccgatag
    40501 gctttcaccc ccctttccat gacatagcag cagcttttca aatacagctc cctacttgct
    40561 ttcacccaag atccggactt ctctttcaag cgtaatacct gagtgttcct tgactttttc
    40621 gataaccgat tggatcaagt cctcgtagtc tttggccgtt ccatctgcga cattgatcat
    40681 aaatcctgca tgcttttctg acacttctac gccaccgata cgatagcctt tcaagccagc
    40741 ttctgaaatt aactgacctg caaaatgccc gactggacgc ttaaagaccg agccacaaga
    40801 tgggtattcc aaaggttgct tgagttcacg taggtgcgtc aagcggtcca tttcctgctt
    40861 gataacctga tgggttcctg gagctagggc aaatttaact gacaagacaa ctgcaccaga
    40921 ctcctgaata gctgaatggc ggtaaccaaa agccaagtct ttagcagaca gggtttcgat
    40981 ttctccatcc ttggtcaaga ccttacaaga ctgcaagatg tgagcaatct cgccaccata
    41041 ggcacccgca ttcataaaga cagcaccgcc aacgcttcct ggaataccac aagcaaactc
    41101 aaagccagtt aaactatgac ggagggcaat gcgagttgtt tcaatcaagt tagccccagc
    41161 ttctgcttca atggtatagc catcaacaga aacgttattg agcttgtcac acaagatgac
    41221 aaatccacga atcccaccat cacgaacgat gatattgctg gcattgccaa gaaccatcca
    41281 agggatattt tcttggttgg caaatttcac aaggcgagcc aactcaaaac gatttcgtgg
    41341 aaagaccaaa taatcagcct ctccacctac ttttgtataa ctatagctat gcaagggttc
    41401 ctgaaaacgg atatcaattc cttctaagat ttcaagcatt ttttctctta cagacatgtc
    41461 actcttcctt ttacaaaatt cattccatta taccattttt agagacattt gacgaccata
    41521 aaaatacctt gtttggattt tgcataagaa aaagaggttt cccccttttt atgatttttt
    41581 acaaaagatt tccttggttc cataggcgac cagaacgagc tccagtgcta gaatcacttc
    41641 aaccaagact ggatttgtca accagcctac ttggaaaaga gatggtgcca gatcaaagaa
    41701 ggcatgcaag ccataggctg ctaggagata aatccatttc ttctggcgaa cagcttggta
    41761 aacccaaact gtcaaaagta attggaaacc aagcgccaag attcgctcaa aaccaagcaa
    41821 ataaatctgc cagaccgaaa gtgactgaat ggtttttaac atattttcag acagtaattg
    41881 cataacctgt ggattctgag tttgaactgc cgaaagaacg atgtaaagat tgagtaaact
    41941 agtaaggcct agaaaaatca actccaagcc accatgccct aggccatagg ccaaggcatc
    42001 tgccttttcc aaacttctct ttttctccaa ccatttgaag aaaacaagac gagccgtttc
    42061 ctcaaaaaat gctgccatgg ctaggccata gataatatag ataagcggat gatcttgcaa
    42121 gagggcaata ctaccgtctt tttgaggatg caaaatcaag atatgcacca gtttttccaa
    42181 aatctgcgaa gagacaaaga aagcgactgc acccaagccc aagacagcta gattaatctg
    42241 gtatttcttt ttggcaaacc aaatgcttcc tatcagaaaa gctagcaaca gcaacatggt
    42301 aataatgata tgaatggtca ttttcttctc ctattctgcc ttttcaatat cttttttcat
    42361 ctcgtcaaca ttgaacttag caaacaagta ttgacggtct tggactggga aacgttggtc
    42421 caactggtca actgctccca cctcgataat gccttccttg atgacaaaat ccatgacatg
    42481 tccaccgaag gtcaaatcat ctgatatgaa gtgcagatgg tagcctgcca cactgacccc
    42541 atggaaaatc tcaggcgtcc agaaaccaac aatagtcccc gcaacattgt cacgactata
    42601 ttccggttga tgggttgcga catcagcaaa cttggtatcg ggtgttgact taggaatcat
    42661 acgcacatgc atatgtgaaa attccccccg aatcttgata gagcggaaaa gattttcccc
    42721 atcataataa gactcaattc gttctttcaa ttccttgtct gtcatctcaa agcgctggcg
    42781 gaaaatgacc tctgcctgat gcggtaccac tgcagcgtaa ggaataaggg catctggtga
    42841 cacttccaca atttctggct ggtctcctga ccctttggcc tgataagcct tgccatccaa
    42901 gacaatcaat tccccatcaa tggaatccaa ggttcccaaa ccaaggtcac catgttctag
    42961 caattctcct actgtcatag taccaccata aaggccagcc atcaaggctc caagagtatt
    43021 gtattgaaat aatttcactg gttcctgcac gttcttatcc attcactttc ttgtctgtta
    43081 ttctataaat cttactccta agtataccac atttgcccct agatgtgaac gagagaaacg
    43141 ctctagacat tgccaagaag gaaaaaaaag ggtacaatgt aacaaaatca agggaggtct
    43201 ggaatgaaga aacaaagcaa gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag
    43261 tctggacgat ttccgacggg tagtcgcctg ccttctatcc gtcaactgag ccttgacttt
    43321 cactgcagca aggacaccat tcaacgagcc ctgctggaat tacggcacga acaatacctc
    43381 tatgccaagc ctcagagtgg ctactatgta ttagaacaag ggcaacatca agacctagaa
    43441 atcgaggtta ccgacgaaca tgccagtgcc tatgacgatt tccgactctg tgtcaatgaa
    43501 accttgattg gacgagaaaa ctacctcttc aactactatg acaatcaaga aggattagaa
    43561 gacctaagac agtccattca caaactcctc tttgagcaag ctctctactg caaggctaac
    43621 caactagtac tgacttctgg aacccaacaa gccttattta tcctctctca aatatccttt
    43681 cctagacaag ccaaggaaat cttggtggaa cagccaacct accatcggat gaatcgcctc
    43741 ttgattgcac aggggctgga ctatcaaacg attgaacgag gcattgatgg gattgacttg
    43801 gaggagctgg aaggccactt caaaacagga aaaattaagt ttttctacac cattccccga
    43861 tttcactatc ctctgggaca ttcctattct gagcaagaca aacgatccat tcttaactta
    43921 gctgccaagt atgatgtcta tatcgtagaa gacgattatc tgggtgattt ggactccaag
    43981 aagggccaaa ccttccacta tcttgataca gaggagcatg tcatttatat caagtccttc
    44041 tcgaccagcc tttttcctgc ccttcgtatt acagcactca ttcttccaaa tgctatcaaa
    44101 gaagcatttg tggcctacaa aaatatccta gactacgaca gcaacctcat tatgcaaaag
    44161 gccctgtcac tctatattga cagtcaattg tttgaaaaaa atcgtttggc tcgcttgacc
    44221 aatcatgaat cttaccaaaa acaaatcgag gaaaggataa ctaaaacacc ttgtcccctt
    44281 cctcattatc ccctacacga tggtttattg ctagacctga gacagtatcc taaaatcgcc
    44341 agtctcaaac acagtcaacc gggcttggac ttctttgaag aggcctattt aagtacctgt
    44401 ccttatcaat ttgccaaggt gtccctagac aatctggaaa atgttttaaa ctatttaaaa
    44461 gcagaattgg aatgacatcc aactctgctt tttcttattc ttcaacttca gttagacttg
    44521 ctgctttttc aagatagctt tggtaaattc cttcatcctt aagtttttgg atgaccttgt
    44581 ctaccacttc tttcaaatca tcactatttt ttctaagagc aacggcattg gcgtcgccgt
    44641 ccttcatctt caagctgaca gttgcgacag ctaagccagc gtttttagca gcataactaa
    44701 gtgcaacagg ctcatccata tgaacagcat ctacttttcc agcctgcaat tcattgactg
    44761 cttcacccat attagttagg gaagttaatt gagcttttgg caattgttcc ttgaccattg
    44821 attctggaac agtccctttt tgggctgcaa tattagcact ttctaggcta gttaaatcct
    44881 tgtatttttc cacatcagcc ttacgaacca agaaactaat cttgttttca tagtatggga
    44941 ttgaaaaatc aaagacttct tttctctcgt cagtagcact aattcctgca actgctaggt
    45001 cagccttacc agtttgaaga ctggtcaaaa cattgtcaaa actcatgctt gagatttcca
    45061 acttaacccc aagttcatca gcgatagcct gagccatgtc gatgtctgca ccgactacct
    45121 ggttctttcc atcaaccaat gattgaaatt caaagggtgc atagtcagga ctggttgcca
    45181 caactaattt ccccttttgc ttaatggcct caacagctga ctgagaacca ttagaacctg
    45241 actggcaagc tactaagaag aaacttgcaa gaaaattaca taaaacaaat atccattttt
    45301 ttgatttcat aaat
//
LOCUS       DAVKLW010000011        41088 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKLW010000011 DAVKLW010000000
VERSION     DAVKLW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.011"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            64..228
                     /locus_tag="VW324_000593"
     CDS             64..228
                     /locus_tag="VW324_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9726271.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            307..1035
                     /gene="yaaA"
                     /locus_tag="VW324_000594"
     CDS             307..1035
                     /gene="yaaA"
                     /locus_tag="VW324_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9726272.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1086..2570)
                     /locus_tag="VW324_000595"
     CDS             complement(1086..2570)
                     /locus_tag="VW324_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9726273.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            2786..9547
                     /locus_tag="VW324_000596"
     CDS             2786..9547
                     /locus_tag="VW324_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9726274.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGVAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKENVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPRVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAKGEKGDRGER
                     GLTGAQGAKGEKGDRGERGLTGAQGAKGEKGAQGERGLTGAKGEKGDQGERGLTGAQG
                     EKGDRGERGLTGAKGEKGAQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTV
                     VRDGFDGASPLVATQRNDADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKG
                     DKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDG
                     ASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGK
                     DGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTL
                     TFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGD
                     TFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEK
                     GDQGERGLTGSKGEKGDQGERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAG
                     PQGPKGPKGEQGNPGTPGKDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITD
                     GRNGTNGQSPTITATRSVQNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTIT
                     EGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYIN
                     TETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVG
                     RDGRDGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGE
                     RGEDGKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQG
                     KNGNPSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKE
                     TKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGS
                     QPTTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGK
                     NGKDGRAPKIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVL
                     NSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITT
                     TENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTN
                     AIIKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRD
                     NGDGSHTIVITNPEGVTTETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRD
                     GREPKLEVIDNNDGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSD
                     GREYKSIIKDGKDGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPK
                     VSVEDNGDGSHTITIINSDGTVTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVT
                     PSNDKPVPPTPNVPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVP
                     EQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVG
                     RRKEA"
     gene            complement(9784..10332)
                     /locus_tag="VW324_000597"
     CDS             complement(9784..10332)
                     /locus_tag="VW324_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9726275.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            10653..11141
                     /locus_tag="VW324_000598"
     CDS             10653..11141
                     /locus_tag="VW324_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9726276.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            11138..12325
                     /locus_tag="VW324_000599"
     CDS             11138..12325
                     /locus_tag="VW324_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9726277.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            12318..12542
                     /locus_tag="VW324_000600"
     CDS             12318..12542
                     /locus_tag="VW324_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9726278.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            12699..14045
                     /gene="asnS"
                     /locus_tag="VW324_000601"
     CDS             12699..14045
                     /gene="asnS"
                     /locus_tag="VW324_000601"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9726279.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPVAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            14198..14488
                     /locus_tag="VW324_000602"
     CDS             14198..14488
                     /locus_tag="VW324_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9726280.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            14500..14970
                     /gene="ssbA"
                     /locus_tag="VW324_000603"
     CDS             14500..14970
                     /gene="ssbA"
                     /locus_tag="VW324_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9726281.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVLDFSRNENPFGATNPLDISDDDLPF"
     gene            15002..15241
                     /locus_tag="VW324_000604"
     CDS             15002..15241
                     /locus_tag="VW324_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9726282.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            15536..16936
                     /locus_tag="VW324_000605"
     CDS             15536..16936
                     /locus_tag="VW324_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9726283.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGNPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            16938..17297
                     /locus_tag="VW324_000606"
     CDS             16938..17297
                     /locus_tag="VW324_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9726284.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            17351..18100
                     /locus_tag="VW324_000607"
     CDS             17351..18100
                     /locus_tag="VW324_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9726285.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            18090..18389
                     /locus_tag="VW324_000608"
     CDS             18090..18389
                     /locus_tag="VW324_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9726286.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            18460..19395
                     /locus_tag="VW324_000609"
     CDS             18460..19395
                     /locus_tag="VW324_000609"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9726287.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            19482..19994
                     /locus_tag="VW324_000610"
     CDS             19482..19994
                     /locus_tag="VW324_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9726288.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDEEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            20019..20627
                     /locus_tag="VW324_000611"
     CDS             20019..20627
                     /locus_tag="VW324_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9726289.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            20728..20910
                     /locus_tag="VW324_000612"
     CDS             20728..20910
                     /locus_tag="VW324_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9726290.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(20956..22401)
                     /locus_tag="VW324_000613"
     CDS             complement(20956..22401)
                     /locus_tag="VW324_000613"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9726291.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            22507..24129
                     /locus_tag="VW324_000614"
     CDS             22507..24129
                     /locus_tag="VW324_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9726292.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            24332..26290
                     /locus_tag="VW324_000615"
     CDS             24332..26290
                     /locus_tag="VW324_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9726293.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            26462..26695
                     /locus_tag="VW324_000616"
     CDS             26462..26695
                     /locus_tag="VW324_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU9726294.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            26701..28143
                     /locus_tag="VW324_000617"
     CDS             26701..28143
                     /locus_tag="VW324_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726295.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRITSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDIICSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            28301..29395
                     /locus_tag="VW324_000618"
     CDS             28301..29395
                     /locus_tag="VW324_000618"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9726296.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            29403..30569
                     /locus_tag="VW324_000619"
     CDS             29403..30569
                     /locus_tag="VW324_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9726297.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            30662..33760
                     /locus_tag="VW324_000620"
     CDS             30662..33760
                     /locus_tag="VW324_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9726298.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKTDLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADNHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVIYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            33808..34425
                     /locus_tag="VW324_000621"
     CDS             33808..34425
                     /locus_tag="VW324_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9726299.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            34436..35770
                     /locus_tag="VW324_000622"
     CDS             34436..35770
                     /locus_tag="VW324_000622"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9726300.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            35780..36244
                     /locus_tag="VW324_000623"
     CDS             35780..36244
                     /locus_tag="VW324_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726301.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            36241..36735
                     /locus_tag="VW324_000624"
     CDS             36241..36735
                     /locus_tag="VW324_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726302.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            36816..38471
                     /gene="mltG"
                     /locus_tag="VW324_000625"
     CDS             36816..38471
                     /gene="mltG"
                     /locus_tag="VW324_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9726303.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            38542..39024
                     /gene="greA"
                     /locus_tag="VW324_000626"
     CDS             38542..39024
                     /gene="greA"
                     /locus_tag="VW324_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9726304.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            39532..39930
                     /locus_tag="VW324_000627"
     CDS             39532..39930
                     /locus_tag="VW324_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726305.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            40375..>41088
                     /locus_tag="VW324_000628"
     CDS             40375..>41088
                     /locus_tag="VW324_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9726306.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNG"
BASE COUNT        13008 a         7508 c         9141 g        11431 t
ORIGIN      
        1 aaaggtttat ttaataaata tatgttataa tagagtaaat taagactaat ggaggacaaa
       61 gaaatgggtt tttatttgat ggttgcttca atgctactcg gactgctcgc tttgaagata
      121 ggtttttctc agttcaagga gaaaaaagat aaattcttat ctatcttaac aagtttagct
      181 ggcacagccc ttgttctcgt ggctgtctgg ttaggatggc ccaaataaat agaaaaacct
      241 cggtcaaacc gaggtttttc aaatgaattt cttggttgtg gctaaaaaat atgctacact
      301 atcaatatga aaattttaat cccaacagca aaagaaatga acacagactt gccaagtatc
      361 gaggcaattc ctttaaaacc agaaagtcag accgtgcttg atgccttggc tctctattct
      421 gccagtcaat tggagagttt ctacaaggta tcagctgaga aagcggcgga agaatttcaa
      481 aatatccaag ctttgaaaag gcaaactgct caacactatc cagccttgaa actttttgat
      541 gggcttatgt accgcaacat taagagagat aagctgaccg aggcggaaca agattatctt
      601 gaaaatcatg tcttcattac ctcggctttg tacggtgttg ttccagcctt gtcacccatg
      661 gctcctcacc gtttggattt tttgatgaaa ttaaaagtcg ctggtaagac tttgaagagc
      721 cattggaagg cagtctatga tgaagctctg aagaaggaag aagtgatttt ctctctcttg
      781 tcatcagagt ttgagactgt attttctaag gaaatcagag caaagatggt gaccttcaaa
      841 ttcatggagg atagaggcgg tcagctgaag attcactcaa ctatctccaa gaaagcgcgc
      901 ggggcctttc taacagcttt aatagaaaat caagtacaaa ctgtggggga agcacgtcgc
      961 ttgaactttg ctggatttgt ttaccgagaa gatttgtcac aaccacaggg attggttttt
     1021 gtaaaggaag tatagaaatc agataagaga aaagtcagca cgattatgct gacttttttg
     1081 ataacttata aagtattgga caaatgaatc tcatttgata aggtgttaag atgattaacc
     1141 aaattcatga ttgacagatg atttcggcaa ataaacttct tggtaatccc tggaatatag
     1201 aaattagaca cgatgatatc gtaatctgtc tgatttaaaa tctcaggact tgtttttaat
     1261 tcatcccaaa tatcgaaggt aaagcggtta ttacagtaat aggaaagcat atcaacaaag
     1321 gtaagggata tagcatggtc aaagttactg ataatcaaaa ccttgatagg tggtcgattt
     1381 tctaacaact gagtagagag gttttcagca tgggttaaga tggtgtacat caagtgttcc
     1441 aactgttcag gatggtcatg ctgtccaatt gcctgacggt attgagccag ttcttgacgc
     1501 gcacttccca taaagtctgg gaagtaaact tcaaattttt taatcgtcaa tgctttttgt
     1561 tcaaataaga ttggggtaga aaagatttct tgtctttcaa agaaagcagt gttgtgtaaa
     1621 tgccaaatca gctcatcgtg gttagtaaaa gtaatcttat attgttttga taggcgttct
     1681 aatatttgac taagtaattg gtaagagtat cttgcttgac tgttatcttc taaagagttg
     1741 aagaattctt gaggatctag gaaaatatca ttctgaagaa aagaaataaa aatttgagcg
     1801 attgtatctg gagtcatttc aagcccaaaa tgtttagaga aataagcaag tgtctcctgg
     1861 aaatttggaa tgctctttaa aagaggataa tattctttgt aaaagtggtt ggggaggtca
     1921 atgaagtgac catttttaac cctgtggata ctaatagcta tcatcagttt gtacattcta
     1981 taaattgaaa atcgcattgg ataattcgtg attttataga agaagtcagc aaactctgtt
     2041 aaatcttcct caggaaggtc aggaaaaggc caatctagga aataatagcg ctcagaaaag
     2101 tattgggcaa aaaagtaacg aatatcgatc tcatttccac gtatctcgga tggagtaaaa
     2161 gataattcaa tttgaaactg agatgaaaga acctttgtaa tatttctgcc cagtcgatag
     2221 aggttggcac tactaaagta aagattttct attgttcgat aaataggtag cccctcattg
     2281 aagaacatat actctagtaa ttgaaaagat tgagaattag caagaaaata ttgataaata
     2341 tcttctacac tagtgttaac ttctaaatct atcatgattc cgttaacaga agattggatg
     2401 ttaatagatg gaaaggcaat acgtaattca tttaaatcac tttttaagat tcgttctgta
     2461 catcctaatt tttcggctag ttcatgtaat ttcatccaat tacgattctg aattagggtt
     2521 tccattaagc gtaattgtct ttgaactttt gtggataaaa ggtttctcat acgggtattt
     2581 cctccataat tcattatatc tttttttgtc taagtagtat aaaaaaatta gagcaagaaa
     2641 attgtcgtta gtaatcggaa aaattagaat aatataagtg gcttttaagt gcagaaacag
     2701 agtatattaa ttgttcagaa aatattgatt aatgcctcaa atagttgtac attgtaaact
     2761 ataaaatact atctaggagt ttaaaatgaa acattcacat aaaaaatcat ttgactggta
     2821 tagcatgcaa caacgttatt ctattcgtaa gtattacttt ggtgcagcta gtgtcttgct
     2881 cggtaccgct ttggtattag gtgtagcagc tagtgtccaa acagtacaag cggaagaaaa
     2941 caaacaagaa actaccaata gtatttctgt tggtagggga gaagcagcta ctaaaccagc
     3001 agaggtttct gcgtctaata aagagaaaac ctatgcagct ccaactgtag ctaatccagt
     3061 agaaacgact ccagttaaaa ctgaagaagt tactaaacca gcagaaaaag ttgaagaagc
     3121 aaaagacaaa aaagaggaag taacgcatca agatgccgtt gacaagtcaa aattattaac
     3181 ggctctttcg cgtgctaaaa aattagaaag caagttatat acagaagcaa gtgctgctaa
     3241 cttgcaaaca agtatccaag ctggtcaaag cttgcttgga aaagcagatg caactgaagc
     3301 tgaattatca gcagcagagt catctattca atcatttatt attggtctag aacttcgttc
     3361 taactctaat aaagaaactg tatcagaaac gcctgtagcg aagaaagctg atgcagttga
     3421 atcaaaagaa ggggctaaac cagctgcaac aactgaacgt tcagctgttg atagcgctat
     3481 tttgccaact agcacagctg acaaagtaga aacaacttca gctccagcat ctattaatga
     3541 aatcttgaaa ctaggtttga gcctttctga tgctcgccaa aatccagcta tccgtaagga
     3601 aaatgttaat agagggtata gtggttttag agcggctagc aatccagcca acccaattgt
     3661 ctcaggttct ggaaatacag ttgcatttgc agatattagc caaggtggtc gtagctatag
     3721 tttccgtggc tacgggaact cacgtggtgg aaattctatt cattacgatg taacaacagt
     3781 acgtagtggt aatagtgtta actttacaat tagttattct gcgccaggag attctagaga
     3841 gtttgttaat aataatttta tcttggataa aggggatgga tttggaaatc cttcaaatgc
     3901 aacgatcaca agttcaaatc caagagtaag ggagcaatca aaatctatta gtcagggtgc
     3961 caactacgta tctcactcag gatacagtat gacctctgct atttcaacaa atacggaaca
     4021 aactatcaga tttagtttgc ctatcattaa tctaaatggt gatttgtctg ttcgtttgaa
     4081 acctgttact tttaacgtgg accaaggtgg tggtggtgcc gctactagca atgacccata
     4141 cagtaactct aactattatt atagagcaaa cccactatac ttggatgcaa acccttatgg
     4201 tggtactaat aataagactg tttcagaaga cattgacttc caaactgtct atcttccaac
     4261 tagtaagtta ccagaaggtc aaactagatt agttcgagaa ggtgaaaaag gacaacgtca
     4321 aattacctat aaagttcatc gatttggtaa cgaaacactt ttaggattgc cgattagtaa
     4381 tagtgttact aaagaagcta agccacgtat tatgcaaatt ggtgtggcta aagatctaat
     4441 cgatacagta aaaccacgtg ttgatcaaaa taaagtcggt gatacaaata acctcacttt
     4501 ctatcttgat aacgatggaa acggtgttta tactgaaggt gtagacgaac ttgttcaaaa
     4561 aattgctatt aaagatggag ctaaaggtga aaaaggagac caaggtgaac gcggtctaac
     4621 tggagctaaa ggtgaaaaag gagaccgagg cgaacgcggt ctaactggag ctcaaggagc
     4681 taaaggtgaa aaaggagacc gaggcgaacg cggtctaact ggagctcaag gagctaaagg
     4741 tgaaaaagga gcccaaggtg aacgcggttt aactggagct aaaggtgaaa aaggggacca
     4801 aggtgaacgc ggtctcactg gagcccaagg tgaaaaagga gaccgaggcg aacgtggttt
     4861 aactggagct aaaggtgaaa aaggagccca aggtcaggca ggtcgtgacg gtgtaactcc
     4921 aaccgtaacc gttaaagata ataaaaatga cggcactcat actatcacta ttaacgacgg
     4981 tagaggtaat gttacaagta ctgttgtaag agatggtttc gatggcgcaa gtccattagt
     5041 tgcgactcaa cgaaatgacg cagataagac aacaactgtt atcttctatt acgataaaaa
     5101 tggaaacaat gaattagatg cttctgataa gaaattaaaa gaagttgtaa ttgcagatgg
     5161 tgctaaaggt gaaaaagggg acaaaggtga acaaggtctt caagggcgtg atggtgaaca
     5221 aggaccaaaa ggagaagatg ggaaaacccc aacagttaaa gtaactgatg gtcaagatgg
     5281 aacgcataca attacaatta acgatggtaa aggtggtata actactacag tagtaagaga
     5341 tggatttgat ggtgcaagcc ctcttgtttc tactcataga aatgaagcag ataaaacaac
     5401 aactgttatt ttctattatg atctaaatga taataatcaa tttgatgaag gagatacaaa
     5461 acttaaagaa gttgttatcg cagatggaaa acaaggacca aaaggtgaca aaggtgataa
     5521 cggaaaagat ggattcacac cagaagtaac agttacagat aacaataacg gaacacacac
     5581 catcacaatc acacaaccag acaacagacc atcattaaca acaatcgtta aaaacggtga
     5641 agatggaaaa acaccaaaag tcaaagcaga acgagatgat gcgaagaaac aaacaacatt
     5701 aacattctac attgacaaag atggagatgg aagttacaca gcaggaaaag acgagttagt
     5761 tcaaacaaca gtagttaaag acggacaaga cggagctgca ggagcatctg gacgtgatgg
     5821 aaaagaagta ttaaacggaa aagtagaccc aacaacagaa ggtaaagatg gagacacatt
     5881 cgtaaataca caaacaggag atgtattcgt taagaaaggt aacacttggg aaccagcagg
     5941 aaacatcaaa ggaccgaaag gtgacaaagg tgcagatggt gccaagggtg aaaaaggagc
     6001 ccaaggagaa cgcggcctga ctggagcgca aggtgtcaag ggtgaaaagg gagatcaggg
     6061 tgaacgcggt ctaactggat ctaaaggtga aaaaggagac caaggtgaac gcggtttaac
     6121 tggagcgcaa ggtgccaaag gtgacaaagg tgaacaaggt cttcaaggtc gtgatggagc
     6181 tcaaggacca aaaggcgcag atggacaaag aggaccagcg ggaccacaag gaccaaaagg
     6241 accaaaagga gaacaaggta atccaggaac tccaggtaaa gatggaaaat ctctaattgc
     6301 tgttaaaaat ggaactgaaa caaaagtcta tgtagaggat ccggctagac caggacaacc
     6361 acttaatcca aaccaaccat tagcaactat cactgatgga agaaacggta ctaatggaca
     6421 atcaccaaca attacagcga cacgaagtgt gcaaaatggc aaaaatggtg tattagtaac
     6481 aattacacca gtaggaggtc gtccacaaac aacatttgta gaggatggac aaaagggtgc
     6541 tgatgggaaa accccaacag taacaataac tgaggggcaa aacggcacac atacattaac
     6601 ggttcataat ccaggaagtc cagatgtgac aactacgatc cgtgatggag ctacaggaca
     6661 agcaggtcgt gatggtaaag atgtattaaa cggaaaagta aatccacaac caaaccaagg
     6721 taaaaatgga gataaatata ttaataccga aaccggtgat gtctatgtta aaaacaatgg
     6781 aaactgggat aaagaaggca acatcaaagg ccctaaaggg gacaaaggtg cagatggtgc
     6841 taaaggcgaa aaaggagacc aaggcgaacg cggcctaact ggagcgcaag gagctaaagg
     6901 tgcggatggc gcagtaggtc gtgatggacg tgacggtaaa gacgtgttga acggcaaagc
     6961 taacccagaa gcacatcaag gtaaagacgg cgataaatac gttaatacag aaacaggcga
     7021 cgtcttcgtt aagaataacg gcaactggga taaagagggc aacatcaaag gccctaaggg
     7081 tgacaaaggt gaacgcggag aagatggtaa gactccagaa gtaactgtaa ctccaggtaa
     7141 agatggccat agtactgaca ttacattcac tgttccaggt aaagatccag ttacagtaaa
     7201 tgttaaggac ggagaaaatg gtctgaacgg taaaactcca aaagttgatt tacttcgtgt
     7261 ccaaggaaaa aacggaaatc catctcatac aattgtgaca ttctatacag atgaaaacaa
     7321 tgacggcaaa tatacaccag gaactgatga acttctaggt tcagaaatga ttaaagatgg
     7381 tgctaaaggc gcggacggac gagatggtaa atcattgctt actgtcaagg atggtaaaga
     7441 aactaaagtt taccaagaag atccagctaa cccaggacaa ccattaaatc cagaaaaacc
     7501 acttgcggta attagagatg gagtagatgg aaaatcacct acagttacag ctgttcgtaa
     7561 agatgaagca gggcataaag gtgtagaaat cactgttgat aaccatgatg gttcacaacc
     7621 aactacagtc tttgttcaag atggtgctaa gggagaaact ggtgcaaccg gtcaggatgg
     7681 acaaactcct acaatcacta ctcaacgtgg acaagatggc caaagcactg ttgtaactat
     7741 cacaacatca ggtaaagatc cagtaacctt cactgtaaaa gatggtaaga atggtaaaga
     7801 tggccgtgca ccgaaaatca aagtagaaga tattacttca ccttcaagaa ttagacgcga
     7861 tacagatgct gctgcaactc caacgcgtaa cggtatccgt gttacagttt atgatgatgt
     7921 taatgacaat ggggtatacg acgaaggtgt cgataaagta ttaaatagta aagatattta
     7981 taacggtata gatggacgtg atggttcagc tccaactatt actacaaaag ataatggaga
     8041 tggaactcac actatcacag ttcaaaaccc agatggttct gaatcaacaa cagttgttaa
     8101 agatggtaaa gacggtaaaa ctgcgaatat cactacaaca gaaaacccag atggaagcca
     8161 cacaattacg gtgacaaatc cagatggttc aactaaagaa actgttgtta aaaacggtaa
     8221 agacggtaag actcctaaag ttgaagtaac ggataacaac gatggaactc atactgttaa
     8281 agttacagat ggagacggca atgttaccaa cgctatcatc aaagatggta aagacggtaa
     8341 agctgcaaca gcaacaacta ctgaaaatcc agatggaagc cacacagtaa caatcactaa
     8401 cccagacgga actaagaatg agtttgttgt taagaatgga cgtgacggtg ttgacggacg
     8461 tactccaacc gcatctgttc gtgataatgg agacggaagt catacaatcg ttattacaaa
     8521 tccagaaggt gtgacaactg aaaccacagt tcgtgatggt aaatcaccaa aagtgactat
     8581 aactgatgaa caaaatggaa ctcataagat ctctgttcta aatggtgacg gaacaactac
     8641 aacaactaca gttcgtgatg gtagagaacc aaaacttgaa gttattgata acaacgatgg
     8701 ttcacacact attaaagtga caggtgctga tggtaaagga acgacaacta caatctttga
     8761 tggtaaatca ccaaaagcga acatcgttga taacggagat ggaactcata cattaacaat
     8821 cgtagattct gatggtcgtg aatacaaatc tattatcaaa gatggtaaag acggcaaaga
     8881 tagcgtttca ccaactgtaa ctgttaaaaa taataacgat ggaactcacg ttgttacaat
     8941 cactaatcca gatggaagta agacagaaat ggtgattaaa gacggtaaag atggtaaatc
     9001 accaaaagtt tctgttgaag ataatggtga tggtagtcat acaatcacaa tcatcaattc
     9061 tgatggaact gtgacaaaaa cagttattaa agatggtaga gatggacgtg atggtcgaga
     9121 cggcaaagac ggtaaagacg gtaaagatgg aaaatgtgga tgccaagaca aaccagtaac
     9181 accatcaaat gacaaaccag ttcctccaac accaaatgtg ccgacaccag aagtaccggt
     9241 taaacctgtg ccagcgcaac caacaccaaa tgtaccgaca ccagaagtgc cagtacaacc
     9301 aactccagct gttccaacac cagaagtacc ggttaaacca gtaccagcgg ttccagaaca
     9361 accagtagta ccaacaccgg ctcaaccagc aactccagta aatgctaacc cagtagcacc
     9421 aactacaggt aaagaaaacc gtggggacaa attacctgaa actggaagcc aatctgatta
     9481 tatctctgtt cttttaggta gcggtattct attgagccta tatgtaggac gaagaaaaga
     9541 agcttaatca gccaaaagca gtcactaatt tctcgaaaga agatgataag tgatagatct
     9601 cgaatgaaag aatattctta gaatgatttt cctatgatgg aaaagactgc taaaaacctt
     9661 gagtggtaac gctcaaggtt tttttggatg gctgatgttt gtctgaaaat cttgattgag
     9721 aaataaaaag ccaccctatt aggtggctat aattgattta gactttctaa aattttctat
     9781 tttttactga atcgcttcga ccaagtcttg agcttgtttt tcaagtgagt ttaggactgt
     9841 ttcttcaaga accaattttc cgtctgccca ggcagagtca ttaacacgtg cagcagtgaa
     9901 atcaccaacg ccttgtgtac ggataaatgg caagaggtct ttgtagatag cgaaaagttg
     9961 atcgtgccct gcattggcta cagatgatac ggtgacaaac ttgtcttgaa gggcagaaac
    10021 gccacgcgta tcagacaagt caagggcacg agatagccag tcaagcaagt tttttactgt
    10081 accagggata gagaagttgt agactggaga gaaaatccag atagcatccg caacgagaac
    10141 tgcttcacga gcagcagcta cagctggatg tgttggaact tccaaatctt ggctgaagag
    10201 aggaagggct gaataatcaa ggtagctaac ttccgcttta ccagcaagtg ctttctcagc
    10261 ttcgagcgcc atttggtggt tgaaagaacc ttgacgtagt gatccgacga taaataatac
    10321 ttttttagac atgatgtgtc tcctttttct tcaaattttg agaactctct cgttgttata
    10381 aaatacatta caacaaaaca aaagaacttg caagaaattt gctcagagtt tatttgaaaa
    10441 ttgtaaaatt tcggaaactc tagtcatttg tgactgaaaa agtaagaact gtttcacttt
    10501 caaaatcggt tttgtttgaa gggagaaata gaattggttg ctcaaagcac agctttgagg
    10561 ttgcagataa agctgacgtg gtttgaagag attttcgaag agtattctta tattcacttg
    10621 taataaagct taatagatgg taaaatagac gagtgaaaaa aagacaaaac aaagcaaaaa
    10681 ataatctact gtggcagtat ggtctaggga tgacgatttt gtttgtggtt atcagtgctt
    10741 cctttctgta tatggtttct cttagcatga aaccctatca aacagctaaa agtgaaggag
    10801 aaaaattagc tcagcagtat gcaggattag agcaggccga tcaggttgat ttatacaatg
    10861 gcttggaatc ttattacagc gttcttggtc gtaataaaca gcaagaagca cttgctgttc
    10921 tgattggaaa agatgatcat aagatttacg tttatcagct aaatcagggt gtttcacaag
    10981 aaaaagcaga aacggtttct aaggaaaagg gagctggcga aattgacaag attatctttg
    11041 gtcgttatca agataagcca atctgggaag tcaagtcagg atctgatttt tatctagtag
    11101 attttgaaac aggagcattg gtcaacaagg agggcctatg aaattatcca accgtgtttt
    11161 agaaatggaa gaaagtgtga ctttggctgc tggagcccgt gccaaagcac tgaaggctga
    11221 gggtagagat attttgtctc taaccttggg tgagccagat ttcactactc ccaaaaatat
    11281 ccaagatgcc gccattgcat cgattcgaga tgggcgtgct tctttttata cagtaacctc
    11341 aggtctgcca gagcttaagg cggcggtcaa tagctacttt gagcgctttt acggctattc
    11401 tgtagcgtca aatcaagtga cagtcgctgc gggagccaaa tattctctct ataccttctt
    11461 tatggctgtg gtcaatccag gtgatgaagt catcatccca accccatact gggttagcta
    11521 tggagatcag gtcaagatgg cagaaggcgt tcccgtcttt gtttctgcta aggaagacaa
    11581 tcactttaag gtgaccgtag agcagttaga agcagctcgc actgacaaga ccaaggtttt
    11641 ggtgctgaat tcgccatcta atcccacagg tatgatttac acccgtgagg aactcttggc
    11701 aattggaaac tgggctgtag aaaatgatat tctcatccta gcagacgata tctatggccg
    11761 cttggtttat aatggtcatg agttcacacc gatttctagc ctatcggaag cgattcgcaa
    11821 gcaaacagtg gtcatcaatg gtgtgtctaa aacctatgcc atgactggtt ggcggattgg
    11881 ttatgccgtt ggagaagcag acattatcgc tgccatgtcc aagattgcag gtcaaacaac
    11941 ttcgaatccg tcagcagtag cccaatatgc agcagttgag gctctatcag gtgagcaaga
    12001 tactgtagaa agcatgcgtc aggcctttga ggaacgtctt aataccatct atcccctcct
    12061 tgcagaggtg ccaggatttg aagtggtcaa accgcaaggg gccttctatc tcttcccaaa
    12121 tgtcaaaaag gccatggaga tgaaaggcta cacggatgtg acagacttta caactgctat
    12181 cttagaagaa gccgaagtgg ccttggtaac aggagctgga tttggagcac cagaaaatgt
    12241 ccgcctcagc tatgcgacag acctagacac gcttaaagaa gcagtcgaac gcttgaaagc
    12301 atttatgggt agtgagaatg attgatcatt ttgagattaa ggtaaaggat ttacaaattt
    12361 cagaaggatt ttataggagt tttctcgctc ctttggacta taaattggct tttaagacta
    12421 gttctctaat tagttttcta tccccgaaca gccctcatcc tggtggtgat ttttggctga
    12481 ctcagggaac acaagatcct gttcattttg cttttttagc agaaaataag gaagaagttt
    12541 aggcttgtta tgaggctggc ttagaggcag gtgggcgaga taatggggct cctggttatc
    12601 gaagtgagcg tccgatttac tatgctgctt ttatgattga cctggatggg aacaatatag
    12661 aggtggtttg ccataaagaa taaaaaagaa aaggaaaaat aatgacaaaa cgtgtaacga
    12721 ttattgacgt aaaagactat gttggtcagg aagtgacgat tggcgcttgg gttgccaata
    12781 aatcaggaaa agggaaaatc gctttcttac aattgcgtga tggaacagcc ttctttcaag
    12841 gtgtggcttt taaaccaaac tttgtcgaaa aatttggtga agaagtggga cttgagaagt
    12901 ttgatgttat caaacgcttg agccaagaaa cgtctgttta tgtgacaggt attgtcaaag
    12961 aggacgaacg ttctaaattt ggctatgagt tggacatcac agacatcgaa gtgatcggtg
    13021 aatctcaaga ctacccaatc acaccaaaag aacacggaac agactttttg atggataacc
    13081 gtcacttgtg gctacgctct cgtaagcaag tagctgtgtt gcaaatccgt aacgctatta
    13141 tctatgcaac ttatgagttc tttgacaaga acggttttat gaagtttgac agcccaattc
    13201 tttcaggaaa tgcggcagaa gattctacag aactctttga aactgactac ttcggaacgc
    13261 cagcctactt gagccaatca ggtcagcttt acctagaagc aggggctatg gctcttggtc
    13321 gtgtctttga ctttggtcca gttttccgtg ctgaaaaatc aaaaacacgc cgtcacttga
    13381 ctgagttctg gatgatggat gctgagtact catacttgac acatgatgag tcgcttgact
    13441 tgcaagaagc ttatgtgaaa gctcttctac aaggtgttct tgaccgcgcg cctcaagcct
    13501 tggaaacctt ggaacgtgat acagaactct tgaaacgcta cattgcagag ccattcaaac
    13561 gtatcactta cgatcaagcc attgacctct tgcaagagca tgaaaatgat gaagatgctg
    13621 actacgagca tcttgagcat ggtgatgact ttgggtcacc acacgaaact tggatttcaa
    13681 accactttgg tgtgccaaca tttgtcatga actatccagt agccatcaag gccttctaca
    13741 tgaaaccagt tcctggaaat ccagagcgcg tgctttgtgc agacttgctt gctccagaag
    13801 gctatggaga aattatcggt gggtctatgc gtgaggaaga ttacgatgcc cttgtcgcta
    13861 agatggatga acttggcatg gatcgtacag aatatgaatt ctaccttgac cttcgtaaat
    13921 acggtacagt tccacacgga ggatttggta tcggtatcga acgtatggta acctttgcag
    13981 caggaacaaa acatatccgt gaagctattc cattcccacg tatgttgcac cgtatcaaac
    14041 cataagaaca ggaaaacgcc catgtggcgt tttttcgaat tctagtgttt ttacttgcgt
    14101 aaaaaaattt tttctagtat aatagtttat tgtgagcgaa cctcacttac cccttgcaaa
    14161 gtcttggggt cattagacca aaaggaggaa catatcaatg gctaaatacg aaattcttta
    14221 tatcattcgt ccaaacattg aagaagaagc taaaaacgct ttggtagcac gttttgactc
    14281 tattttgact gacaacggtg caactgttgt tgaatcaaaa acttgggaaa aacgtcgtct
    14341 tgcatacgaa atccaagatt tccgtgaagg actttaccac atcgttaacg ttgaagcaaa
    14401 tgacgatgca gctcttaaag agtttgaccg tctttcaaaa atcaacgctg acattcttcg
    14461 tcacatgatc gtcaaaattg acgcgtaaga aggtaaacta tgattaacaa tgttgtactt
    14521 gtagggcgta tgacacgtga cgctgagttg cgttataccc catcaaatgt agcagttgcg
    14581 acttttactc ttgcagtaaa ccgtacattt aagagtcaaa atggtgaacg tgaggctgat
    14641 tttatcaatg tcgttatgtg gcgccaacag gctgaaaatc ttgctaactg ggctaaaaaa
    14701 ggctcactta tcggggtgac aggtcgtatc cagactcgta gttacgataa ccagcaagga
    14761 caacgtgtct acgtgacaga ggtcgtggct gagaatttcc aaatgttgga aagccgtagt
    14821 gtgcgtgagg gtcacacagg tggagcttac tctgcaccaa ctgcaaacta ttcagcacct
    14881 acaaattcag tactagactt ttcacgtaat gaaaatccat ttggagcaac aaacccattg
    14941 gatatttcag atgatgattt accattctaa tggacaacta aacttataaa ggagaaaaaa
    15001 catggctcaa caacgtcgtg gcggattcaa acgccgtaaa aaagttgatt acatcgcagc
    15061 aaacaaaatt gaatatgttg attacaaaga tactgagctt cttagccgtt tcgtttcaga
    15121 acgtgggaaa atccttcctc gtcgtgtaac aggaacttca gctaaaaatc aacgtaaagt
    15181 aacaacagct atcaaacgcg ctcgcgtaat ggctttgatg cctttcgtaa acgaagatta
    15241 aagaaaagac ccggttgggt ctctttttca tgagcttgaa aacaagaaag cgaattaaga
    15301 ccccaatggg tcttttttca caaactttga aatgagagag cgagttaggc tcagtggctc
    15361 cctttttacg agtttgaaaa caagagaaca cattaaaagc acgatggagc tttttcataa
    15421 atatgtcttt tttctcatcc aagaaattaa aataaaaatg ataaactttt tgaaaatagg
    15481 ggatttgcct actttatgat ataatggaaa gagttagatt gaaagaggta gtgagatgga
    15541 cgcgaaatta agatacaagg caaagaagat caagattgtc ttttttgata ttgatgatac
    15601 attgcggaat tcaaagacag ggtttattcc aactacaatt cccactgttt ttaaacagtt
    15661 gcgtgaaaaa ggaattttaa caggaatcgc ctctggacgt ggcatttttg gtgttgttcc
    15721 agagattcgt gatctcaagc ctgacttttt tgtaactttg aatggggctt atatcgaaga
    15781 taaaaaaggt caggtcattt atcagcatca gattgaaaag tcagatgttg aggagtatat
    15841 ctcttgggct aagcaagaag gaattgagta tggcttggtt gggagtcatg atgccaagtt
    15901 gtcgactcgc accgatatga tgagtgaagc tatcaatcca atttatcccg acttagatgt
    15961 agatcccgat ttccatgaaa aagaagatat ctatcagatg tggacttttg aagataaggg
    16021 agatgacttg cacttgcctg acagtctctc agacaaactt cgcatggttc gttggcatca
    16081 acattcgtct gatattgtgc cgatttcagg ctccaaagcg acgggggtgg aaaaagttgt
    16141 ggaacacctt ggcttgaaac cagagaaggt catggttttt ggagatggtc tcaacgactt
    16201 ggaactcttt gattatgctg gaatcagcgt tgccatggga atttctcatg ataaaatcaa
    16261 agaaaaagca gattatatta caaaaacatt agaagaagat ggcatttttg atgccttaga
    16321 agtatttggt atggtagaaa aagaattgca ttttccacaa gtagacattg aaacagtaga
    16381 aggtcctctt gcgactatta agaccaatca cggagactta cgtatcaagc ttttccctga
    16441 acatgctcct aaaacagtgg ctaactttgt atctctttca aaagatggct actatgatgg
    16501 tgtcattttc caccgtatta tcaaggactt tatgatccaa ggtggaaacc caactggaac
    16561 tggtatgggt ggcgagtcaa tctacggcga atcttttgag gatgaattct cagaagagct
    16621 ttacaatatc cgtggtgctc tttccatggc aaatgctggt ccaaatacca acggcagcca
    16681 gttctttatc gtgcaaaacc aacacctacc ttattctaag aaagaaatta ctcgtggtgg
    16741 ttggccagaa ccgattgcag aaatctatgc caatcaaggt gggacacctc acctagaccg
    16801 tcgtcacacg gtttttggtc agttagctga tgaagcatct tacgctgtct tggatgccat
    16861 tgctgctgtt gagacaggag ctatggacaa gccagttgaa gatgttgtaa ttgaaactat
    16921 tgaaatcgag gactaagatg aaaatcggtg ataagctaaa ggggcgtatt acagggattc
    16981 agccctacgg tgcctttgtt gagttagaga cgggtgatac ggggctgatt cacatttcag
    17041 aaattcggac aggatttatt gaaaatattc atgaaacctt gaaagtcgat gaagaggttc
    17101 aagttcaggt agtggattta gatgaattta cggggaaagc cagtctttct atccgcactt
    17161 tggaggaaga aaagtaccag tttcctagac ggagacgctt ttcaagcgac cgctttaact
    17221 acggttttgc tccctttcga cgaatgctac caatctggac tggagaagcc ctgcaccatt
    17281 taaaaaagaa gaagtagttt agagttttat attttttgta atgttaatat aaatttgcta
    17341 taataggacc atggaagaag aacaattatt aaaatcagga gagcgcatta accagctctt
    17401 ttcgacagat atcaaaatca ttcaaaatag agaggttttt agctattcgg tggatagtgt
    17461 tctcttatca cgatttccac gttttcctaa gaaggggttg attgtggatt tctgtgctgg
    17521 gaatggagca gtggggcttt ttgctagcac tcgtactcaa gcacagatat tgtctgttga
    17581 gattcaggag cgtttggcgg atatggctga acgctctgtc cgtttgaatg gattagagga
    17641 gcaaatgcag gtcatctgcg atgatttgaa aaatatgcct gctcacattc aggggagtaa
    17701 ggtggatatg attttgtgta atccacccta tttcaaggtg gatccttatt ctaatctgaa
    17761 cgagagtgaa cattatctct tggctcgaca cgaaatcacg actaatttgg aagaaatctg
    17821 tcatagtgcc cagagtattc tcaagtctaa tgggcgtttg gccatggttc atcgtcctga
    17881 tcgacttctg gatattttgg atacgttaaa acgacataat ctagccccta agcgcctgca
    17941 gtttgtttat ccaaaaagag aaaaggaagc caatatgctt ttgattgagg cgatcaagga
    18001 tggctcaaca agtggcttta aggtcttacc tcctttgatt gtccacaatg atgatggctc
    18061 ttatacgccc gaaatcgaag agatttatta tggatcataa ggcttatatg tacgttctgg
    18121 agtgtcgtga tggatcctac tatataggct atacgactga tatgagaaga cgcctcgcta
    18181 tccacaatag tgggaaggga gccaaatata cacgagcacg cttgccagtc aaacttatct
    18241 atgctcaagg ttttgccagt aaggaagaag ccatgtcggc tgaagctctt ctcaagcgta
    18301 agaagaggcc acagaaggaa gaatttttat ctgaaaatca agatagaaat ttactcagtt
    18361 attttgaaga atcgtgggga gtcctttgac tcctcttacc tttgtcaaaa agcgaaaaaa
    18421 atgctacaat aaagaaaata aacaaaatga ggtattatca tgtccaagat tctagtattt
    18481 ggtcaccaaa atccagactc agatgccatc ggatcatctg tagcttttgc ctaccttgca
    18541 aaagaagctt acggtttgga tacggaagct gttgcccttg gaactccaaa tgaagaaaca
    18601 gcctttgtct tgaactattt tggtgtggaa gcaccacgtg ttatcacttc tgccaaagca
    18661 gagggggcag agcaagttat cttgactgac cacaatgaat tccaacaatc tgtatcagat
    18721 atcgctgaag tagaagttta cggtgttgta gaccaccacc gtgtggctaa ctttgaaact
    18781 gcaagcccac tttacatgcg tttggagcca gttggatcag cgtcttcaat cgtttaccgt
    18841 atgttcaaag aacatggtgt agctgtgcct aaagagattg caggtttgat gctttcaggt
    18901 ttgatttcag atacccttct tttgaaatca ccaacaacac acccaacaga taaaatcatt
    18961 gctcctgaat tggctgaatt ggctggtgtg aacttggaag aatatggttt ggcaatgttg
    19021 aaagctggta ccaacttggc tagcaaatct gctgaagaat tgattgacat cgatgctaag
    19081 acttttgaac tcaacggaaa taatgtccgt gttgcccaag tgaacacagt tgacatcgct
    19141 gaagttttgg aacgccaagc agaaattgaa gctgcaatgc aagctgccaa cgaatcaaac
    19201 ggctactctg actttgtctt gatgattaca gatatcgtca actcaaactc agaaatattg
    19261 gctcttggtg ccaatatgga caaggtcgaa gcggctttca atttcaaact tgaaaacaat
    19321 catgccttcc ttgctggtgc cgtttcacgt aagaaacaag tggtacctca attaactgaa
    19381 agctttaatg cgtaagattt tgggtgtcag ctcaaaatcg gaaagtctag tttgccttat
    19441 atcgaaagga gtttcggctc ctttttttct aggagtgaag tatgttagaa aatggcgatt
    19501 tgatttttgt gagagatggg tcagacatgg gacaggccat ccagacttcc acaggtaact
    19561 atagccatgt tgccatttat ttggatggga tgatttatca tgctagtgga caggctggtg
    19621 ttgtctgtca agaaccggca gacttctttg agtccaatca tttgtacgac ctctatgttt
    19681 acccagaaat ggatatccag tcggtgaagg aaagagcttg cagacatctt ggagcaccct
    19741 acaatgcttc tttctatcca gatgcagctg gtttctactg ctcccagtat atagcagaaa
    19801 tcctacctat ttttgaaact attcctatga aatttggaga tgaggaacag gagattagtg
    19861 atttttggag ggagtattac atagaactag gtctgcctgt tcctctgaac caagctggga
    19921 ccaatcctag tcagttggca gcatcgcctc tgttacaatg taaagaaagg aatcttcatg
    19981 attcagattt ttaatccatc tcgtttgacg agacagccat ttttggagaa ttgatccgct
    20041 atctggacca gtatgaggat gtgattctac gggaaattaa ggctcaattt ccagatgttg
    20101 cagttgataa actcatggaa gagtatataa aggcaagctt gattctacgt gaaaataagc
    20161 gctattacct taattttcct acgcttgaat cacttgatag tcttgaactg gatcaagaga
    20221 tttttgtcag agaagctagt ccggtctatc aagccttgtt ggagcagagt tttgagacgg
    20281 aattgcgcaa tcaaatcaat gcagctattt tagttgaaaa gacggacttt gcgcgcatta
    20341 aaatgaccct gtccaattat ttttacaagg tcaaacagca gtatcctttg acagaaaaac
    20401 agcaggagct ctatgacatt ttaggagatg ttaatcctga gtatgccctc aagtatatga
    20461 cggctttttt gttgaaattt ctcaaaaaag accagcttat gcagaaatgc cgtgatatct
    20521 ttgtggacag tttggttgtc ttaggctata ttgtgcaaaa tgaagatgga aagtatgagt
    20581 tggctatcga ttttgataag gagagattaa ctttctactt agcgtgattt cttgtttctg
    20641 agtacattgt ttgactttcc ttagtattcg gtataaacta tatgtaaccg gtaacacata
    20701 tcggaataaa ctaaaggaga caatcatatg tcacttgaaa acaaattgga acaagcaaca
    20761 ggcgctgtca aagaaggttt tggtaaagtt actggagaca gcaagacaga acttgaagga
    20821 gctgttgaaa aaacagttgc taaggcaaaa gacgttgtag aagacgcaaa aggtgctgta
    20881 gaaggtttga aaaacgtttt tactaaataa taggaaaaaa tcaagggttt cattttccct
    20941 tgattttttc tattcttata aataattttc tgcgacggct gtatctcctg ggtaggattc
    21001 tttcttgccc tggatgattt ggtaacaatc gactccctta cccgcaataa taactgcatc
    21061 taattcgtga tttgtgatag ccattgccgc cttgatggct tcttggcgat ccgcaatctt
    21121 ttcaacagga tgattgatgt agctactaat ttcatctgca atggccattg ggtcttcata
    21181 gttagggtca tcagcagtca gaaagacttg aatctcaggg tgttgattga ggaggaggcc
    21241 aaagtcctta cgacgacttt ctcccttgtt tcctgttgat cccagaacca gagcaatctt
    21301 tccggtttga tgagtttcca ccacattgat gagttttttc agactatccc cattgtgggc
    21361 atagtcgatg aagaccttgg ctccattttt ctgagtgagg acttccatac gaccaggaac
    21421 gcgggttgca gcgatgcctt ttttgatgtc ctcaagactt gctccgagac ggagacaagc
    21481 aagtccagca gcaactgcat tttcttggtt gaagttgcca atgagttgga tatcataatc
    21541 tccagcgagt ttacccgtag ctgaaaagct aaaggctttg gaattctcga tttggttatc
    21601 aaattggcta ccatagaaat catggtcttg atcttcaacc tgttctttca agactgagaa
    21661 gtggtccatg tcactgttaa tgatgactgc tcggctattt tccatcaaga gacgcttgtg
    21721 gtagaaatag tcttcaaagc tagggtgttc aatcgggccg atatggtctg ggctgatatt
    21781 gaggaaaact cccacatcaa aggttagacc atagacacgt ttgaccagat aggattgact
    21841 ggagacttcc atgatgaggt gggtacggtc attttgcaca gcctgattca tcatgtcaaa
    21901 gaggtcaata ctctcagggg ttgtcaacgc tgacttaaag aaagtctcgc catcaagagt
    21961 tgtgttcatg gtcgacaaca tagcaggtct atgcccttga gataagatgt tataggcgaa
    22021 ataggttgct gttgtcttac ccttagtacc agtaaaggca aggagtttga gtttctcttg
    22081 tggattacca tagaactcca tggcaatcaa actcatggct ttctttatat cgttcacaat
    22141 gatgacaggg atatcgactt cgtagtcctt ttcagctaca taccaagcta aaccttgtgt
    22201 tatagcagaa agaaggtatt cttttttaaa ggcagcgcct tttgcaaaaa aaagagtgtc
    22261 ttctgttact tttcggctgt cgtagctgat gctatcaaaa acaactttgc tgtagttgta
    22321 gtggtaatga ccttggtcaa taatttcgcg aaaaaggcca tctttcttta aaatatctaa
    22381 tacggtttca atcttaatca tactttctat tgtaaaccga aagtcgcaaa tttacaagta
    22441 acaaggaaaa gtttataatg gaagataagg agtttttcct agttatcaaa attgaatgag
    22501 gaatctatgt cgcacgaaaa caatcaccag caggcccaga tgttacgggg gactgcttgg
    22561 ctaacggcta gtaactttat cagtcgccta ctcggggctg tttacattat cccttggtac
    22621 atctggatgg gggcttatgc agctaaggca aatggtctct ttaccatggg ttacaatatc
    22681 tatgcttggt tcttgttggt ttcaacagcg gggattccag ttgcggtggc caagcaagtt
    22741 gccaagtata ataccatgcg agaagaagag catagctttg ccctgattcg gagcttctta
    22801 ggctttatga caggactagg cctggttttt gctttagtct tgtatgtctt tgctccttgg
    22861 ctagcagact tgtctggcgt gggcaaagac ttgatcccaa tcatgcaaag cttggcttgg
    22921 ggagtcttga ttttcccgtc tatgagtgtt atccgaggat ttttccaagg gatgaataac
    22981 ctcaaaccct atgccatgag ccaaattgct gagcaggtca ttcgtgttat ctggatgctc
    23041 ctagcaacct ttatcattat gaagctcggt tcaggagatt atctagcagc cgttacccaa
    23101 tcaacctttg ctgcctttgt cggtatggta gccagttttg cagtcttgat ttatttcctt
    23161 gcccaagaag gttcactcaa aagagtcttt gaaacaggag ataagattaa cagtaagcgt
    23221 ctcttggttg ataccattaa ggaagccatt ccttttatcc tgacagggtc tgccatccag
    23281 ctcttccaga ttttggatca gctgaccttt atcaatagta tgagctggtt taccaactac
    23341 agcaatgagg acttggttgt catgttttct tatttctcag ccaatcctaa taaaatcacg
    23401 atgattttga tttctgtagg ggtttcgatt gggagtgttg gtttgccact tttgacggaa
    23461 aactatgtca agggggactt gaaagcagct tctcgtctgg ttcaggacag tctcacccta
    23521 ctctttatgt tcttgctacc agcaacggtt ggagtggtta tggtaggaga acctctttat
    23581 acggtcttct atggtaagcc agatagtttg gctctgggct tatttgtctt tgcagttttg
    23641 cagtctatta ttttaggctt gtacatggtc ttgtctccaa tgcttcaggc catgttccgc
    23701 aaccgcaagg ccgttctcta ttttatctat ggttctattg ccaagctagt cttgcaacta
    23761 cctaccatcg ccctcttcca cagttatggt cctttgattt caacaaccat tgctctcatc
    23821 attcctaacg tcttgatgta tcgggatatt tgtaaagtaa ctggtgtcaa gcgcaaggtg
    23881 attttgaagc gaaccatttt aatcagtttg ctgaccctag tcatgtttct gttaatagga
    23941 accatccagt ggctgttagg atttttcttc caaccaagtg gacgtttgtg gagcttcttt
    24001 tatgtagctc ttgtcggtgc catggggggt ggactttata tggttatgag tctgcgtacc
    24061 tatttattag ataaggtaat aggaaaagcc caagcagatc gcctgcgagc aaaatttaag
    24121 ctttcgtaag gtagcattta taaataaaag gaataagttg aacaatcttt taggagaact
    24181 tgaaacttat tcctttttta gcgtaaaaat agctaaaatt atctttcaaa aatagaaaat
    24241 gaggtttttt tcgatttttt ggaaaaataa ttgactaaac aaaccaaacg ttgtataata
    24301 aaacaaatat ttaaagcagg aggttctgga aatgaaaaag tctaagagca aatatctaac
    24361 cttggcaggt cttgtcctgg gtacaggagt tttattgagc gcgtgtggaa attctagcac
    24421 ggcgtcaaaa acctacaact atgtttattc aagtgatcca tctagcttga actatctagc
    24481 agaaaaccgc gcagcaacat ccgatattgt tgcaaatttg gtagacgggt tattagaaaa
    24541 tgaccaatat gggaatatta ttccatcatt agcagaggat tggactgttt ctcaggacgg
    24601 tttgacctat acctacaaac ttcgtaagga tgccaagtgg tttacttctg agggagaaga
    24661 atatgcgcct gtaactgccc aggattttgt gacaggtttg caatatgcag ctgataaaaa
    24721 atcagaagcc ttgtatctag tgcaggactc tgttgctggt ttggatgact atatcactgg
    24781 taaaacaagc gacttttcaa ctgtcggtgt caaggcactt gatgaccaaa cggttcaata
    24841 tactttggtt aaaccagaac tttactggaa ttcaaaaaca cttgcaacga tactttttcc
    24901 tgttaatgca gatttcctga aatcaaaagg ggatgatttt gggaaggcgg atccatctag
    24961 tattttgtac aatggacctt tcttgatgaa agcacttgtc tcaaaatctg ctattgaata
    25021 taagaaaaac cctaattact gggatgctaa gaatgtcttt gtagacgatg tgaaattgac
    25081 ctactatgat ggtagcgatc aagaatcact ggaacgtaat tttacagctg gtgcttatac
    25141 tacggctcgt ctttttccta acagctccag ctatgaaggg attaaagaaa aatacaaaaa
    25201 caatatcatc tatagtatgc aaaattcaac ttcatatttc tttaatttta acctagatag
    25261 gaagtcttac aattatactt ctaaaacaag tgacattgaa aagaaatcga ctcaggaagc
    25321 agttctcaat aaaaacttcc gtcaggctat caattttgct tttgacagaa catcttatgg
    25381 ggctcagtct gaagggaaag aaggtgcaac aaagattttg cgtaacctag tggttcctcc
    25441 aaactttgtc agtatcaagg gaaaagactt tggtgaagtt gtagcctcta agatggtcaa
    25501 ctatggtaag gaatggcaag gtatcaactt tgcggatggt caagaccctt actacaatcc
    25561 tgagaaagcc aaggctaagt ttgcggaagc taagaaagaa ctcgaagcaa agggtgttca
    25621 attcccaatc cacttggata agactgtgga agtaacagat aaagtaggca tacaaggagt
    25681 tagttctatc aaacaatcaa ttgaatctgt tttaggttct gataatgtag tgattgacat
    25741 tcagcaatta acatcagatg agtttgacag ttcaggctac tttgctcaaa cagctgctca
    25801 gaaagactat gatttatatc atggcggttg gggacctgat tatcaagacc cgtcaaccta
    25861 tctcgatatt tttaatacta atagtggagg agttctacaa aatcttggac tagagcctgg
    25921 agaagccaat gacaaggcta aggaagttgg gctggatgtc tatactcaaa tgttggaaga
    25981 agctaataaa gagcaagatc cagccaaacg ttatgagaaa tatgctgata ttcaagcttg
    26041 gttgattgat agttctttag ttcttccaag tgtttcgcgt gggggaacac catcattgag
    26101 aagaaccgta ccatttgctg ctgcctatgg tttaaccggt acaaaagggg ttgaatcata
    26161 taaatacctc aaagtacaag ataagattgt cacaacagac gaatatgcaa aagccagaga
    26221 aaaatggttg aaagaaaaag aagaatccaa taaaaaagcc caagaagaat tggcaaaaca
    26281 tgtcaaataa aatctaattg tagataagtt tgtgtaagaa gtctttgtgc ttaaaaacgc
    26341 ataatatcag gtatataaaa tcctagatgt tatgcgtttt gttataggaa gatttattag
    26401 attttatcct caaattgagt tattcccgac tttatccgtt gaaatattat aaggagtatt
    26461 tatgaaatta aaattattaa gagtagatac taaggtgatt atggggagtt tcttccttgt
    26521 tctgtctagt ctacttgctt tgttgcttcc ccttatctta aaaggtttaa tagatgggag
    26581 ttctattgaa aatataggtt ccaaagtatt tcaatcgttt ttgattttta ttggtcaagc
    26641 cttgttttct tctattggtt actatctgtt tagtcaatcg ggtgaaaaaa gatagcaaaa
    26701 atcaggaaaa aagtgataga ggggttgatt tatgcagaga aatccttctt tgataagagc
    26761 caaagtgggg agttgacttc tgccattgtc aatgacacga gtgtcattcg tgagttttta
    26821 attacgactt tcccaaatat tattctgagt ttagttatgg tacttggttc cattgtagtc
    26881 ttatttagtc ttgattggaa tctttctcta cttttattca tcactcttcc ttgtatgatg
    26941 tttattatct tgcccctttc caatatcagt gaaaagtata gtcgtcgttt acaggaggaa
    27001 atcggatttt taacagggca attgactgaa aagattcaag aacatgaatt aattaagact
    27061 aaccaagcag agaaaagtgt acaggatgtt ttggataatt gtattgaaag ggtacaaaac
    27121 aattcactga aatcagatag gattacttct tttgagaccc cgtttgctct cttatttatc
    27181 tttgcgacta tagctgtgat gctaacctat gggggttatc gggtcagcgc aggatatata
    27241 tctgtgggaa ccttggtttc gtttttgatt tacctctttc aattacttaa tcctattagt
    27301 aatatagcta attttgtaac tgtttattct aggagcaagg gatcttcagt tgcactggag
    27361 aacttgcttg cagttcctaa agaaaaattt gagggaggaa aatcggtatc aggacgaggg
    27421 ttgaatttta accatgtcta ttttggttat gatgaaaatc gacctgtctt aaaggatatt
    27481 atttgttcaa ttttcaaggg gcaaaaaatt gcttttgttg gaccatctgg atcaggaaaa
    27541 tcaacgattg tgcgtttgtt agagcggttt tataaaccgc tttcaggaga tattctaatg
    27601 gagcaatcaa gtatatatga ttttaactta aaagaatgga gaagtaaaat cgcttgggtt
    27661 tcacaaaata atgcagtctt atctggcagt attcgtgaca atctttgtct cggtttgaat
    27721 cgcttagtaa ctgatgatga attgatgaaa gtgctagact tagtatcact aggtgatgag
    27781 attcgctcca tgaaagaggg actagatact gaagttggtg aacgcggacg actcttgtca
    27841 ggggggcaaa gccaaagact tcaaatagct agagcctact taaaagatgc tgaaattctt
    27901 atatttgatg aagccactgc taatcttgat gcggattctg agtatgcgat tatcagtagc
    27961 ctctattctg tattaaagga gaagacggtt gtgattatag cgcatcgttt gtcaacggta
    28021 aaagatgtgg attgtatttt cttcttagag gaggggaaaa tcactggctc aggaactcat
    28081 aaggaactac tggaaaatca tgagcgttat gctcgttttg tgcaggagca aatgatagag
    28141 tgaaggtgtc ttttgagatt caccatttta tagtctatta aagggagcag gaaaaactcc
    28201 ctttttatat agtttgaaac tataactagc tcttgaaaac aagaaaacga aggatacaaa
    28261 ataagtggta caatagttac tatagatttg gaggaattgt atgagcaagg aattacacat
    28321 taacacaatt ttagcacaag cagggatcaa gtctgatgag gcaacaggtg ctttggtgac
    28381 accacttcat ttttcaacga cctatcagca tccagagttt ggtcgatcta ctgggtttga
    28441 ctatacgcgc actaaaaatc caactcgtag taaggctgag gaagtcttgg cggctattga
    28501 gtcagcagac tatgccctag cgactagctc agggatgtca gctattgtac tggcctttag
    28561 cgtctttcca gtaggaagta aggtcttggc agtgcgtgat ctttacggtg gttcttttcg
    28621 ctggtttaac caagtggagc aggaaggtca tttccatttt acctatgcca atacagaaga
    28681 agagttgatt gccgagttag aaaaggatgt ggatgttctc tatatcgaaa ccccaaccaa
    28741 tcccttgatg ttggaatttg atatcgaaaa actagcaaaa ttggctcatg ctaagggttc
    28801 caaagtggtg gtggacaata ccttctatag ccctatttac caacgtccga ttgaagatgg
    28861 agcagatatc gttctccatt cagcaaccaa gtatctagca ggccacaatg atgtcttggc
    28921 tggagtggtt gtgaccaata gtttagaact atacgagaag cttttttaca atcttaatac
    28981 aacaggggca gtcttgtctc catttgacag ctaccagttg cttcgtggtc tcaagacctt
    29041 gtctcttcgt atggagcgct caacagctaa cgcccaagaa gtggttgcct ttttgaagga
    29101 ttctccagca gttaaggaag ttctctacac tggtcgtgga ggcatgattt cctttaaagt
    29161 agccgatgaa acacgcattc ctcatatttt gaacagtctc aaggtcttct cttttgcgga
    29221 aagtttgggc ggagtggaaa gtcttattac ttatccaacg actcaaactc atgctgatat
    29281 tccagcagaa gtacgccatt cttatggttt gacagatgac ctcttgcgtt tgtctattgg
    29341 gattgaggat gctagagatt tgattgcaga tttgcgccaa gccttagaag gataagacaa
    29401 agatgggaaa atatgatttt acaagcctgc ccaaccgttt agggcaccat acctataaat
    29461 ggaaagaaac agaaacggat agtgaagttc taccagcttg gatagcggat atggactttg
    29521 tggtcttgcc tgaaatccgc caagccgtgc aaacttacgc agaccaactg gtttatggtt
    29581 atacctatgc cagtgaagac ttaattaagg aagttcaaaa gtgggaagct acacaatacg
    29641 gttacaactt tgacaaagag gctcttgtct ttatcgaggg tgtggtacca gccatctcaa
    29701 cagctattca aacctttaca aaagaaggcg aggcggtttt aattaacacg cctgtctacc
    29761 caccctttgc tcgcagtgtc aagttgaata atcgtagatt gattactaat tccttagtgg
    29821 aaaaggatag tctgtttgag attgactttg accaacttga aaaggatttg gtggaagagg
    29881 aggttaaact ctatgttctt tgcaaccctc acaatcctgg tggacgtgtt tgggaaaaag
    29941 aagtgttgga gaagattggc caactctgcc aaaaacacgg tgttttgtta gtttcggatg
    30001 agattcacca agatttgacc ctctttggtc acaaacacca gtctttcaat accatcaatc
    30061 ctgccttcaa aaattttgct atcgtcttga gcagtgccac taaaacattt aatattgctg
    30121 gaacaaaaaa ttcctatgca gtcattgaaa atcctaagtt gagactggct ttccagaaac
    30181 gcctgttggc caataatcag catgaaattt caggcttggg ttatttggcg acagaagctg
    30241 cctatcgata cggtaaagat tggctagagg aactcaagca agtctttgaa gaccacatca
    30301 attatgtggt ggatctattt ggaaaagaga ctaaaatcaa ggtcatgaaa ccgcaaggta
    30361 cctacttgat ttggcttgac ttttcagcct atgacctgac tgatgaaaca ttgcaagagt
    30421 tgttgagaaa tgaagccaag gttatcctca accgtggttt ggattttgga gaggaaggaa
    30481 gtctccatgc ccgcatcaat atagctatgc ccaaatctct gttgcaagaa gtctgtcagc
    30541 ggattgtggc tacttttgcc aaatgttaaa aatccagcct tctaggagaa aagtcttcct
    30601 agaaggctat tttcataggc gaaaatatgg tataataaaa agataaggta aaggtgaaaa
    30661 tatggctaaa ttgattccgg ggaaaattcg tatcgaaggt gttgcccttt atgaaactgg
    30721 taaggttgat atcatcaagg aaaagaacaa tcggctctac gctcgcgttg cagaagaaga
    30781 actgcgctat agtttagagg atgatttggt tttttgtgcc tgtgattttt ttcaaaagag
    30841 gggctactgt gtgcatttgg cagcgctaga gcattttctg aaaaatgatg agcgtggtca
    30901 ggaaatcttg tggagtctgg aagaaggtca tgaagaaaaa gaggccgttg aaaccaaggt
    30961 gaccttgggt ggcaagtttt tggatcgaat tttatctcca aaatcagaat gcgcctatga
    31021 gttatcagct gtgggtcagg tggaagcagg aaccaatcat atcttgtgga ctctgagaat
    31081 cggccaaatc aatagccaaa aatactatgt cattcgggat attccacttt ttttaaggat
    31141 tgtcgagcag agaaagtcgt atatgattgg caagatttat gaggagtctc tttcttggga
    31201 agcctttgat gatgccagcc aagaactttt gactttcttg cacggactaa cggaggaagg
    31261 gctggttcca gacctctttt tccaaaacca aggtcggcac ctctttttcc ctctgacctt
    31321 ttttgagcag ggtgtgaatt tactgatggc cttgccccat tttcaatttg accatcaagt
    31381 ggatagctac caaaccctgc tttttcagga tatgcatgct gatgccaatc tatttgcctt
    31441 tacagtaaca gaatattcgg actattttga aatggaaatc agtgagagtc ctagggtcaa
    31501 tgtcttttat caaggagcag tacttttcca taaaggtcag gtttattttt taacagacca
    31561 gcagatgcgt cttctcaagg aaattaaagc gttacctgtg gatcgacaag gaaagaaata
    31621 cctgcaattt gatagcagtg atcgagacaa attggcctcc tgtctgactc tttttatcca
    31681 gatgggcacc gtttcagctc cagaacgtct acaaatcaaa acttttgcgc cctcttttta
    31741 ctttgacagg gaagaagata atcggattcg tttagaaatt cagtttgatt atggaaatag
    31801 acaggtatct agccgacaag agctagaaga attaccattt tcaagtgacg cggacttaga
    31861 agaaagaatt ttccaagtct gtttggctgc gggctttgaa gccgattttc aatcttggcg
    31921 tcaggcctta aaagcggagt ctgtctatca tttttttcat gatatcattc cagtctttga
    31981 aaaactcggg catgttgacc tatcagataa actagaagag ctttatagcc tagcgagtcc
    32041 tcaggtgcag attgcctcca agggaggtct cttggaaatc cagtttgatt ttcaagatat
    32101 tgcccaggag gaaatcgacc aagccataca ggccttggtt gccaatcagg atttttatat
    32161 tgattcgtct aatcaagttt actttttcga tgaagaaacc aagaaaattc gccaaaatct
    32221 acaggaactg gggcaatttg aattaaaaga tgggaccttg caggctcgga aatccttagc
    32281 ctacagttta gctcatctct ttgaagggcg agaccgtgtt tctttttcac aagaattcca
    32341 gaatctggcc caggatttga cacatccaga ggactttcct ttgcaagcaa ctcaggtcaa
    32401 gactgacttg cgagattatc aggaaaaggg aattggctgg ttgcagatgc tccatcatta
    32461 tggttttggt gggattttgg ctgatgatat gggacttggg aaaacccttc agaccattgc
    32521 ttttttgact agtcaagtga caaaagaaag tcgagtcttg attctcgctc cgtcgggttt
    32581 gatttacaac tgggcggatg agtttcagaa atttgctcca cagttggatg tggctgttgt
    32641 tcatggtttg aaagcaagtc gtgaggagat tcttgccgat aaccatcaaa tctatgtgac
    32701 tagctatgcc accttccgtc aggacagtga gctttatcag gggatggcct ttgacttcct
    32761 tttcttagat gaagctcagg tcatgaaaaa tgcccagacc aagattgccc agaccttgag
    32821 acaatttgtg gtgccgtcgg tctttgcctt gtcaggaact ccgattgaaa accatctggg
    32881 tgagttgtgg tctattttcc aaatcgtcat gcccggactt ttgccaagca agaaagaatt
    32941 tatgaaattg ccagcagagc gagtggctca gtttatcaag cctttcgtga tgcgacgcaa
    33001 gaaagaagaa gttctgactg aattgccaga cttgattgag gtgatttata aaaatgaact
    33061 ggaagaccag caaaaggcta tttaccttgc ccagttacaa cagatgcgag accatctggc
    33121 tcaagtgtca gaacaggaat ttcagcgaag tcgtgtggaa attttatctg gtttgatgcg
    33181 cttgcgtcaa atatgtgaca ctcctgccct gtttatggaa gattatcagg gagccagcgg
    33241 caaactagat agtctccgag acctactggt acaggtggca gacggcggac accgtgtctt
    33301 gattttctct cagttcaagg gaatgttgga aaaaattgaa caagaactgc cagacttggg
    33361 cctgacttcc tttaaaatta cgggttcaac cccagccaag gaaagacaag acatgaccaa
    33421 ggcctttaac caaggagaaa gagatgcctt tctgatttcc ctcaaggctg gtggggtcgg
    33481 tctgaacctg acaggtgcag atacagtgat cttggttgac ctttggtgga atcctgcggt
    33541 ggaagcgcaa gccattggcc gtgcccatcg gatgggtcag gaagaaacgg ttgaggtcta
    33601 tcgcttggtg accaagggaa ccattgaaga aaaaattcag gaactccaag aacaaaagaa
    33661 acatctggtg tcacaagtat tggacggcac agagtcacgt ggtagtctga ccctagcaga
    33721 aattagagaa attttgggaa tttctgaagc cagcacttga aaaatcaaga caatacgcta
    33781 taatgaagtg ttgaaaggaa atagaaaatg aaacaagaac gatttccatt ggtgtcagat
    33841 gacgaggtca tgttgactga aatgccagtc atgaatctct atgatgagtc tgatctgatc
    33901 agtaatatca agggtgagta tcgagataaa aattatttag aatgggctcc tattgctgaa
    33961 gaaaaaccag taaaaccgat tgaaaagcaa gtagggaaac ctaaaaaagc tcctttaggg
    34021 gttaaaaaag aaggaaagag ctatgcggag gtggcgcgtg aagaagcgcg tgcagacttg
    34081 aaaaagaaac gctctgctaa ctatctaact caggatttca gccttgcgag acgtcattct
    34141 cagcccagtc tagttagaca gggcaatcaa ccgacaactc ctttccaaaa ggaaaatcct
    34201 ggtgaatttg tcaaatatag ccaaaaattg acccagtctc attatatctt ggcggaagaa
    34261 gttcattcta tccctaccaa gaatgaagaa gtgtcagcac ctgctccaaa gaaaaacaat
    34321 tatgattttc taaagaagag ccaaatctac aataaaaaaa gtaaacaaac agaacaagaa
    34381 cgtcgggttg cccaagagtt gaatctgacc agaatgacag aataggggag aaaacatgtc
    34441 aaagacatat cattttatcg gaattaaggg atcagggatg agtgccttgg ccttgatgtt
    34501 gtaccagatg gggcacaagg ttcagggatc agatgttgaa aagtactact ttacccaacg
    34561 cggtcttgag caggcaggaa ttaccattct tccttttgat gaaaagaatc tagacggtga
    34621 tatggaaatt atcgctggaa atgcctttcg tccagataac aacgtcgaaa ttgcctatgc
    34681 ggaccaaaat ggtatcagct acaaacgtta ccatgagttt ctaggtagct ttatgcgtga
    34741 ctttgttagc atgggagtag caggagcaca tggaaaaact tcaacgacag gtatgttgtc
    34801 tcatgtcttg tctcacatta cagataccag cttcttgatt ggagatggga caggtcgtgg
    34861 ttcggccaat gccaaatatt ttgtctttga atctgacgaa tatgagcgtc acttcatgcc
    34921 ttaccaccca gaatactcta ttatcaccaa cattgacttt gaccatccag attatttcac
    34981 aagtctcgag gatgttttca atgcctttaa cgactatgcc aaacaaatca ccaagggtct
    35041 ttttgtctat ggtgaagatg ctgaattgcg taagattacg tctgatgcac caatttatta
    35101 ttatggtttt gaagctgaag gcaatgactt tgtagctagt gatcttcttc gttcaacaac
    35161 tggttcaacc ttcaccgttc atttccgtgg acaaaacttg gggcaattcc acattccaac
    35221 ctttggtcgt cacaatatca tgaatgcgac agccgttatt ggtcttcttt acacagcagg
    35281 atttgatttg aacttggtgc gtgagcactt gaaaacattt tccggtgtta aacgtcgttt
    35341 cactgagaaa attgtcaatg atacagtgat tatcgatgac tttgcccatc atccaacaga
    35401 aattattgcg accttggatg cggctcgtca gaaataccca agcaaggaaa ttgtagcagt
    35461 ctttcaaccg cataccttta caagaaccat tgccttgttg gacgactttg cccatgcttt
    35521 aaaccaagca gatgctgttt atctagcgca aatttatggc tcggctcgtg aagtagatca
    35581 tggtgacgtt aaggtagaag acctagccaa caaaatcaac aaaaaacacc aagtgattac
    35641 tgttgaaaat gtttctccac tcctagacca tgacaatgct gtttacgtct ttatgggagc
    35701 aggagacatc caaacctatg aatactcatt tgagcgtctc ttgtctaact tgacaagcaa
    35761 tgttcaatag gatgttccca tggaaattcc aattaagatc attcaggcaa gcaagtctga
    35821 tttgcctgag ataggggcac ttcagacctc gtcttttcca gctgaaaagc agcaactttc
    35881 ccatatttta gaagaaagta tccgtaagtg tgcggatacc tttcttctag ctagggatga
    35941 aaatcaactt ttaggctata ttttatcaag tccccagtca gacaatccgc aatatctaaa
    36001 agtacattct ttagtcatcg agtctgacca tcagagacag ggcttgggaa cacttcttct
    36061 tgcagccttg aaagaggtgg cagttgagct ggattacaaa gggattcgtt tggagagtcc
    36121 tgatgagctg ctttcctatt ttgaaatgaa cggttttgtt gatgaagaag caactttgct
    36181 ctatgcaact agccagggct atagtatgat ttggtttaat cccttttatc tggaggaaca
    36241 atgaaaatca gacaagcaag attagaagat ttggatcgga ttgttgaact tgaatttgaa
    36301 aatttttcgg tcgaagaagc cattcctcct tctgtctttg aagcacattt gagagaaatt
    36361 cagacctctt ttctggttgc tgaaaaagaa ggaagaatca tgggttatat cgaaggacca
    36421 gttggcctgc accgccatct gcaagaccag tcttttacag aagaaataaa agactatagt
    36481 catgagcctg gtggttatat ctctgtgacc tgtctgtcta ttgccaagga ggcacaggga
    36541 ctcggactgg gtcagaaatt gctgacagcc ttgaaagaag ttgctcttga agatgagaga
    36601 gatggcatta atctaacctg tcatgactat ctcatcgcct actatgaaaa acatggattt
    36661 gtcaacgaag gccagtccca gtcaaccttt gcaggggaaa catggtatga tatggtctgg
    36721 gaaatgaaaa aataagttag gaaaagtatc atagattgct ttttttggac ttttaagata
    36781 taatattatg gattatcaac aaggaggaaa aacttttgag tgaaaagtca agagaagaag
    36841 agaaattaag ctttaaagag cagattctga gagatttaga aaaagtaaaa ggctatgatg
    36901 aagttctgaa agaagatgag gcagtagttc gcactcctgc aaatgaacct tcaactgaag
    36961 aactcatggc tgattccttg tcaacggtag aggagattat gagaaaagct cctaccgtgc
    37021 ctactcaccc aagtcaaggt gtaccagctt ctccagcaga tgagattcaa agagaaactc
    37081 ctggtgttcc aagtcatcca agtcaagatg taccttcttc tccagcggaa gaaagtggat
    37141 caagaccagg tccaggtcct gttagaccta agaaacttga aagagaatac aatgaaaccc
    37201 caacaagggt agctgtttcc tatacgacgg cagagaaaaa agcagaacaa gcaggtccag
    37261 aaacacctac gcctgctaca gaaacagtgg atatcatcag agatacatca cgtcgtagcc
    37321 gtagagaagg agcaaaaccc gttaagccta agaaagagaa gaagtcacat gtgaaagctt
    37381 ttgtgatttc attccttgta ttccttgcct tgctctcagc aggtggttac tttggttacc
    37441 agtacgtgct agattcctta ttacctatcg atgctaattc taagaaatat gtgacggttg
    37501 gaattccaga aggttcaaac gttcaagaaa tcggtacgac gcttgaaaaa gctggtttgg
    37561 taaagcatgg tctgattttt agtttttatg ccaagtataa aaattatacc gacttgaaag
    37621 caggttacta caatttgcaa aagagtatga gtacagaaga cttactcaaa gagttgcaaa
    37681 aaggtggaac agatgaaccg caagaacctg tacttgcgac tttgacaatt ccagaaggtt
    37741 ataccttgga tcagattgct caagctgtgg gtcaattgca aggtgacttc aaagagtctt
    37801 tgacagcgga gactttcttg gctaaagttc aagatgagac gtttatcagt caagcagtag
    37861 cgaaatatcc tactttactg gaaagtttgc ctgtaaaaga cagcggtgcg cgttatcgtt
    37921 tggaaggata ccttttccca gctacatact ctatcaagga aagcacaact attgagagct
    37981 tgattgatga gatgttagct gctatggata agaacctatc tccttactat agtactatca
    38041 aatctaaaaa cttgactgtc aatgagttgt tgaccattgc ttccttggtc gaaaaagaag
    38101 gtgccaagac agaagatcgt aagctcattg caggtgtatt ctacaatcgt ttgaatcgtg
    38161 atatgccact tcaaagtaat attgcaatct tgtatgccca aggaaaactg gggcaaaata
    38221 tcagtctagc tgaggatgtt gcgattgata ccaacattga ttcaccttat aatgtttata
    38281 aaaatgtagg tctcatgcct ggtccagtcg atagtccaag tctggatgcg attgagtcaa
    38341 gcatcaatca aactaagagc gataacctct actttgtagc agatgtcaca gaaggcaagg
    38401 tctactatgc taacaatcaa gaagaccacg accgcaatgt cgctgaacat gtcaacagca
    38461 aattaaacta aacaaactaa aattatgtta tacttcacat aattttcttt agaaaatatt
    38521 atcagaagaa agttgagaaa aatggcagaa aaaacatatc ctatgaccct tgaggaaaag
    38581 gaaaaacttg aaaaagaatt agaagaattg aaattggttc gtcgaccaga agtggtagaa
    38641 cgcattaaga ttgcccgttc atacggtgac ctttcagaaa acagtgagta cgaagcagct
    38701 aaggatgaac aagcctttgt cgaaggacaa atctctagct tagaaacaaa aatccgctat
    38761 gctgaaatcg tcaatagcga cgcagttgcc caggacgaag tagcgattgg taaaacagtc
    38821 accatccaag aaattggtga ggacgaagaa gaagtttata ttatcgtagg ttcagctggt
    38881 gcggatgcct ttgcaggtaa ggtttcaaat gaaagcccaa ttgggcaggc cttgattggc
    38941 aagaaaacag gtgatacagc aaccattgaa acgcctgttg gtagctatga tgtaaaaatc
    39001 ttgaaggttg aaaaaacagc ctaaaaacag aaaaaggagt ggggaggcga tgtgcttcac
    39061 tcactccttt ttccattttg ctattcttcg aaaatctctt caaaccacgt cagcgtcgcc
    39121 ttgccgtatg tatggttact gacttcgtca gtttcatcta caacctcaaa accatgtttt
    39181 gagccgactt cgtcagtttc atctacaacc tcaaaaccat gctttgagct gacttcgtca
    39241 gtttcatcta caacctcaaa accatgcttt gagctgactt cgtcagtttc atctacaacc
    39301 tcaaaaccat gctttgagct gacttcgtca gttctatcta caacctcaaa acagtgtttt
    39361 gagcaacctg cggctagttt cctagtttgc tctttgattt ttattgagta ttagatgtat
    39421 agtgcattga agttaaaata gtacactttg atttctaaga cattgttaga aattagatta
    39481 aattccttaa tcgatttgcg catgttttat ttcatttcac tatactttag attggaaaat
    39541 aaggaatcac ataaatcgca agaaataggg aatctgattc gtgtttataa ccgttcaaaa
    39601 agagaagagg ctgaaagtga gccacttaat ctttatgtcg aagatgaaaa gggcaatctc
    39661 ctggcaggtt tgatagcaga gacttttgga aattggctag aaatcgagta tttgtttgta
    39721 aaagaggaac tgcgagggca aggaatcggt tcaaaactat tgcaacaggc agaaagtgaa
    39781 gctaagaatc gaaattgttg ttttgccttt gtcaatactt accagttcca ggcaccggac
    39841 ttttatcaga aacatggcta caaggaagtt ttttctttgc aagactatct ctacattagg
    39901 caaagatatt attaccaaaa gaatctgtaa ggtgagtatg gaggtataat acaaagagag
    39961 gctcgcacat aagtatggag gtggactgat gtatatgagt aactatcgat aaataccagt
    40021 tataacctag aaattgtgtc tttctagacg atattgagga caatataatc gtagccccaa
    40081 aattaggaat caagacttat caggttaaga aaagaagtga tgttgttgat attttaaaca
    40141 atttatttaa actaaaaact ctctatctat taatccgaga ttcccctctc ggattttgtg
    40201 ttgctttcat taaatttaca ggtaagactt gctagcttca gtaaatatac acaagttttt
    40261 cagaatagag tgaaatccaa aagaaaatgg aaataatgaa agctcttgta ggcataagaa
    40321 aacacctctg tgttatactt gttgttcaac cacaaacaca agaaaggcac agagatgcaa
    40381 gacaattata ctacaaaagc caaacatttg acaatcgata gccgtcgctt aatcgaaaga
    40441 tggaaaaaag aagggaaatc aaatagagaa attgcctctc tgcttggaaa agttcctcaa
    40501 actatccaca ctgaaatcaa gcgtgggaca gtccgacaat gtcttggaaa agggcgcttc
    40561 aaagaggttt attctgccga ctatgctcaa cagtcttatg aaaataatcg caagcgctcg
    40621 gtcaagaaat caagcttgac caaggaacta aaggaaaaga ttctccacta tcataaccaa
    40681 aaattttcgc ctgaaatgat ggtgatggct aaaggggtta acgtgggaat ttcaaccatt
    40741 tactattgga ttcatcatgg aaaattgggg ttaagcaaac aggatctgct ttaccctaga
    40801 aaaggaaaag cgcttaagaa acaggctagc accaacttta aacctgctgg tcaatccatc
    40861 gaacagcggt ctgaagctat caatcttcgc ttggagaatg ggcattatga gattgatacg
    40921 gttctactta cgagagcgaa aaactactgc ttgcttgtct tgacggatcg aaagagtaga
    40981 catcagatca tccgattgat tccaaataaa agtgctgagg tggtcaatca ggctctaaaa
    41041 ctcatcttaa aacaacacaa gattctttcc atcacggcag ataatgga
//
LOCUS       DAVKLW010000012        39350 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKLW010000012 DAVKLW010000000
VERSION     DAVKLW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.012"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(153..1250)
                     /locus_tag="VW324_000629"
     CDS             complement(153..1250)
                     /locus_tag="VW324_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9726307.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARESDLPEGIHVLGFTEKGRQHLKYLKGQVSLVSRIGKEPWDV
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
     gene            complement(1260..2006)
                     /locus_tag="VW324_000630"
     CDS             complement(1260..2006)
                     /locus_tag="VW324_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9726308.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2109..2462)
                     /gene="rsfS"
                     /locus_tag="VW324_000631"
     CDS             complement(2109..2462)
                     /gene="rsfS"
                     /locus_tag="VW324_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9726309.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2475..2975)
                     /locus_tag="VW324_000632"
     CDS             complement(2475..2975)
                     /locus_tag="VW324_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9726310.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKIAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(2976..3569)
                     /gene="yqeK"
                     /locus_tag="VW324_000633"
     CDS             complement(2976..3569)
                     /gene="yqeK"
                     /locus_tag="VW324_000633"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9726311.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPGLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREVASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(3569..4198)
                     /locus_tag="VW324_000634"
     CDS             complement(3569..4198)
                     /locus_tag="VW324_000634"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9726312.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLVPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4250..4561)
                     /gene="yhbY"
                     /locus_tag="VW324_000635"
     CDS             complement(4250..4561)
                     /gene="yhbY"
                     /locus_tag="VW324_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9726313.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(4775..5881)
                     /gene="yqeH"
                     /locus_tag="VW324_000636"
     CDS             complement(4775..5881)
                     /gene="yqeH"
                     /locus_tag="VW324_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9726314.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAYEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(5884..6474)
                     /locus_tag="VW324_000637"
     CDS             complement(5884..6474)
                     /locus_tag="VW324_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9726315.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(6426..7334)
                     /locus_tag="VW324_000638"
     CDS             complement(6426..7334)
                     /locus_tag="VW324_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9726316.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7391..7951)
                     /locus_tag="VW324_000639"
     CDS             complement(7391..7951)
                     /locus_tag="VW324_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9726317.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8090..9305
                     /gene="sstT"
                     /locus_tag="VW324_000640"
                     /pseudo
     CDS             8090..9305
                     /gene="sstT"
                     /locus_tag="VW324_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9373..10326
                     /locus_tag="VW324_000641"
     CDS             9373..10326
                     /locus_tag="VW324_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9726318.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(10424..10501)
                     /locus_tag="VW324_000642"
                     /pseudo
     CDS             complement(10424..10501)
                     /locus_tag="VW324_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10562..10786)
                     /locus_tag="VW324_000643"
     CDS             complement(10562..10786)
                     /locus_tag="VW324_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU9726319.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(10791..10970)
                     /gene="asp4"
                     /locus_tag="VW324_000644"
     CDS             complement(10791..10970)
                     /gene="asp4"
                     /locus_tag="VW324_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU9726320.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(10972..12309)
                     /gene="gtfB"
                     /locus_tag="VW324_000645"
     CDS             complement(10972..12309)
                     /gene="gtfB"
                     /locus_tag="VW324_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU9726321.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(12302..13813)
                     /gene="gtfA"
                     /locus_tag="VW324_000646"
     CDS             complement(12302..13813)
                     /gene="gtfA"
                     /locus_tag="VW324_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU9726322.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEVQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(13825..16197)
                     /gene="secA2"
                     /locus_tag="VW324_000647"
     CDS             complement(13825..16197)
                     /gene="secA2"
                     /locus_tag="VW324_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU9726323.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(16190..16630)
                     /gene="asp3"
                     /locus_tag="VW324_000648"
     CDS             complement(16190..16630)
                     /gene="asp3"
                     /locus_tag="VW324_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU9726324.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKVKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(16627..18162)
                     /gene="asp2"
                     /locus_tag="VW324_000649"
     CDS             complement(16627..18162)
                     /gene="asp2"
                     /locus_tag="VW324_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU9726325.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(18146..19726)
                     /gene="asp1"
                     /locus_tag="VW324_000650"
     CDS             complement(18146..19726)
                     /gene="asp1"
                     /locus_tag="VW324_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU9726326.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(19736..20953)
                     /gene="secY2"
                     /locus_tag="VW324_000651"
     CDS             complement(19736..20953)
                     /gene="secY2"
                     /locus_tag="VW324_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU9726327.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     RMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(21010..21687)
                     /locus_tag="VW324_000652"
     CDS             complement(21010..21687)
                     /locus_tag="VW324_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9726328.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(21672..21917)
                     /locus_tag="VW324_000653"
     CDS             complement(21672..21917)
                     /locus_tag="VW324_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9726329.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGGQHKLKIWGLV"
     gene            complement(21917..23113)
                     /locus_tag="VW324_000654"
     CDS             complement(21917..23113)
                     /locus_tag="VW324_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9726330.1"
                     /translation="MRKSIVLAADNVYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNSGVLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(23122..24342)
                     /locus_tag="VW324_000655"
     CDS             complement(23122..24342)
                     /locus_tag="VW324_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU9726331.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(24367..25251)
                     /locus_tag="VW324_000656"
     CDS             complement(24367..25251)
                     /locus_tag="VW324_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9726332.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDDWVTEEYLSI
                     LIETLKEQHSEIVVSTYSTYNESDGLFYIHVFDSDYYVKNYNSKLLMEELPLLERYDM
                     SFLTSWGILFKRELFQEVQFPFGRVCEDIGTNYKLFMQVEKVTYINKVLYWYRVGKEG
                     LSNSYSPKMMRDDCDFRLERIAVLALKGYDVSKYLDQMKFYLKYRHDIAIQRELKENV
                     ETRHLEMLDYLLNGNKYN"
     gene            complement(25248..27701)
                     /locus_tag="VW324_000657"
     CDS             complement(25248..27701)
                     /locus_tag="VW324_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU9726333.1"
                     /translation="MIKTIVLAGNRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIASMLGSEIIDVKLPEQTVFQDWKKQAHISSIAYARYFIPDYIQEDTVLYLD
                     CDLLINDKLDSLFEQDVREHYIAAIRDANGQGFNTGVLLINNEKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWNQILQHHLGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLAYHLSRKGYQVLDIGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIQF
                     IEDETYNSQILVDLSKREV"
     gene            complement(27711..28721)
                     /locus_tag="VW324_000658"
     CDS             complement(27711..28721)
                     /locus_tag="VW324_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9726334.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKRTFFAGSLERFPDLQNWSQDTPLRVFSNKGEAGSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFVANSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(28750..29625)
                     /locus_tag="VW324_000659"
     CDS             complement(28750..29625)
                     /locus_tag="VW324_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9726335.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVVAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(29630..30844)
                     /locus_tag="VW324_000660"
     CDS             complement(29630..30844)
                     /locus_tag="VW324_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9726336.1"
                     /translation="MYRKVIALGADIHYLDKVETVIKSVSVHNHEVKFYVFNDDLPSE
                     WFLLMRNRLKVIGSEIINVKKADHNLRDFHLPNAILSYATFFRYFIADEVLEDRVLYL
                     DSDIIVNANLDELFFLDLQGYAIAAVQDFDQDGWLTTFNSGMLLIDAKKWREKKSTQN
                     LLELTAQHHEHVYGDQGVLNIYFGDQWLHLDKEYNFMVGLDQFLHLKGNADWYQSNYY
                     GNYEPKIVHYTTEFKPWTHLTLTRFRKLWWFYYGLNWNDVLLSSDIVKRSFKELVGVP
                     LYHTCIFTNSAAMESLEYLLSELPEVHFTILAHTNFAPSVVDLQRYLNLSLFPNFNSF
                     NMKETLDKMDFYLDINHEGEIVNIIEEVCRRNKPIFSFDNTSHDSKGRSRIFSATEPD
                     KMVDAIRKFLGE"
     gene            complement(31201..32079)
                     /locus_tag="VW324_000661"
     CDS             complement(31201..32079)
                     /locus_tag="VW324_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU9726337.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLSNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVACRNFENFHVSSGKLILNNGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKMRV"
     gene            complement(32076..33476)
                     /locus_tag="VW324_000662"
     CDS             complement(32076..33476)
                     /locus_tag="VW324_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9726338.1"
                     /translation="MHALVDVMSERITLLANMGYPLEKHLAIYRQMLEFSLANGQASG
                     LSDTATYKEFEMKKRLLNQLTVQESSEKKAIVLAANYAYVDQVLTTIRSICYHNRSLR
                     FYLIHSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFV
                     QEDKALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNA
                     FWKKENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQ
                     LPEGQDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIY
                     PIKEPFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNG
                     IHYLVDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYA
                     VDQVQAMIEKLREISK"
     gene            complement(33523..39306)
                     /locus_tag="VW324_000663"
     CDS             complement(33523..39306)
                     /locus_tag="VW324_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU9726339.1"
                     /translation="MFFRRQKGEYRETDRVTRFKLIKSGKHWLRASTSLFGLFKVMRG
                     GVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVISGTVATQTKVFTNESAVLEKTV
                     EKTDALATNGTVVLGTISTSNSASSTSLSASESASTSASESASTSASTSASTSASESA
                     STSASTSISASSTVVGSQTAAATEATAKKVEEDRKKPASDYVASVTNVNLQSYAKRRK
                     RSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIAKSETKVYTGKGVDSVYNVPIY
                     YRLTVTNDGLKLTFTYTVTYVDPKTKTLGNISKKMSNGYSIYNTGTSIQTMLTLGSGL
                     GTPSDVKNSITNKNGGQVQYYNTSTMTKRGSRYTWGNGAQMNGWQAKREYGLTSSWTV
                     PITGTDTSFTFTPYAAKTDNIRTNYFNGRGKVVESSTTSQSLSQSKSLSVSASQSASA
                     SASTSASASASTSASASASTSASASTSASASASTSASASASTSASESASTSASASAST
                     SASASASTSASASASTSASASASTSASASASTSASVSASTSASASASTSASASASTSA
                     SESASTSASESASTSASASASTSASASASTSASASASTSASESASTSASASASTSASA
                     SASTSASESASTSASASASTSASESASTSASASASTSASASASTSASASASTSASESA
                     STSASASASTSASASASTSASASASTSASASASTSASESASTSASESASTSASASAST
                     SASASASTSASVSASTSASESASTSASASASTSASASASTSASASASTSASASASTSA
                     SVSASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASE
                     SASTSASASASTSASESASTSASASASTSASVSASTSASASASTSASASASTSASESA
                     STSASASASTSASASASTSASASASTSASTSASTSASASASTSASASASTSASASAST
                     SASASASTSASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSA
                     SASASTSASASASTSASALASTSASASASTSASASASTSASESASTSASASASTSASA
                     SASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASA
                     STSASTSASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST
                     SASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASASTSA
                     SASASTSASALASTSASASASTSASASASTSASESASTSASASASTSASESASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASA
                     STSTSASESASTSASASASTSASASASTSASASASTSASASASTSASTSASTSASESA
                     STSASASASTSASASASASTSASVSASTSASESASTSASASASTSASESASTSASALA
                     STSASASASTSASASASTSASASASTSASESASTSASASASTSASVSASTSASASAST
                     SASESASTSASASASTSASASASTSASASASTSASASALTSASESASTSASASASTSA
                     SASASTSASASASTSASASASTSASASASTSASTSASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASESASTSASASASTSASVSASTSASASA
                     STSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASAST
                     SASASASTSASESASTSASASASTSASASASTSASESASTSASASASTSASASASTSA
                     SASASTSASESASTSASASASTSASASASESASTSASASTSAGKSRQQLPNTGTEASK
                     SSVLLGALAAVTGLGLFAKRRKRDDEE"
BASE COUNT        11538 a         8656 c         7231 g        11925 t
ORIGIN      
        1 gcggattcaa agataaagat taaggttcat atactaattt tctttattga ctttttgtct
       61 tctgtattag ggagtttatc attttccaaa aactttatct tcaatttgtt agctaaaatt
      121 tctacaataa aaaagcctac ctttcagtag acttagtttg tttctattct aatcggcact
      181 ctgccaaaat tctgttctgc tatacttgga tttcccaatt ggtaaatctg gtctgccttt
      241 tgagtcatga catcccaagg ttccttgcca attctgctga ctagactgac ctgccctttc
      301 agatatttaa gatgttgcct gcctttttcg gtaaagccaa ggacatgaat cccttctggc
      361 aagtcacttt ctctagcctg caccaagata taggtcaaga ggcgtctgac acgcgccttg
      421 gtgtaacgtt tggtggtcac taactcgacc aattcttcca cagactgggc tgttttaata
      481 gcttctttaa tgcgcacagc catttcttga ttgacctgat agatggtggt taggtcggga
      541 tttgacaaga tttgatagcg gagcaaggga aaatagtctt cccagatcac cttactggcc
      601 tgctcaaata gggcaacaga aggcataaaa cgttctaaga aatcttggtc cttctgatgt
      661 tgacggaggg ctgtcgccga ggcaaagtcc acatctttat tcacagaatg gtaacctgcc
      721 ccctgacgct gaatcggatg cagcttgata tttcgtcctg caaccgcctt tgcataggcc
      781 agagcaagaa catgattggg ggtattacct gaaaaatcaa gaccagcaaa ttccttccac
      841 atagcttggg ttttctgggg ataggaaagg gaatcaggca gattttccac aaatttctcc
      901 atctcagcac ctttctctgt gtataagtca gcgattttct gataatctcg aacttcctct
      961 gtcccaaaaa ctagactatc aatgcccaag cgatccaaga tatccacagc tccttgacca
     1021 aagaaatcag ctgcctgaac actgactaaa aagggcaatt ccactaccaa gtctgcgcca
     1081 ttttccagcg ccatctgggc ccttgtccac ttatccacaa tagcaggctc tccacgctgc
     1141 atgaaattcc ctgacatggc aacaattttc agtccctctg cctgatccag caggtattta
     1201 tggccattat gaaaaggatt gaactccgcg ataatacctg tgatggtcat gctaatctcc
     1261 tacttctgcg ccacaaaaaa ccaacgggtg ctagtttctg ttggctcctt atcctcaaag
     1321 tctgcataga gtttgaagga cttgaatcca gcctgttcca gcaagatatc ataggtcaag
     1381 atctcatagg tcctctcctc atgtacttca tcgtggcgac taaaggaacc gtcagcctcc
     1441 ttgataaaga aagtcagctc atgcacgatg gagtgaggaa cttcgtcctc atatgtatcc
     1501 caaagcatgg caaaatcttc cgcattttca tggtaggaat agccagggaa tacttcatct
     1561 gtctggtagg tcgagtgcac gtcaaagatg aaaactccct cttcattcag cgcattgtac
     1621 acgtccttaa agacgtcccc tacttccacc tcatcctgca tatagcagat agagtccgaa
     1681 taacacgtga caaaatcgta tttccctgcc ttggacaaat ttagcatatt accttctata
     1741 aaagcaatct tttgcttggc tgaagttgct ctcttctccg caatcttcaa catatccgca
     1801 ctcaagtcaa gtccagtcac atcaaaacca gcctgagaga agcgcactga ttgaattcct
     1861 gtcccacaag ccaattccaa gagtttcttt ctctccttgg tcttaggcaa atgacgcaga
     1921 gaaaaattcg tccatttgtc gtataaacta tcgtccatga ccgcatcata gacagccgca
     1981 aaggtttcat aagtcgccat aatcatgata ctaagagctc catggtaaac accactagcc
     2041 ctttaccttt ctatcaattt ttctaaaata agcaaagaaa cccagttgac tacaactgag
     2101 ttcatcttct aagcaagagc ttctgaaata tctactgaat tcgcctcatg ccatagcttc
     2161 tctagattat aatgggcacg catttcttct gaaaagatat gcacgacaac agcaccgagg
     2221 tccagtaaga cccagcctcc agctgcatcg ccttcgacat ggctaccttt aaagcctgct
     2281 tgagctactt tttcacggat attagctgcg atagcgtcca gctgacggct attcattgag
     2341 ctagtgatga caaagtagtc cgtcacacta gtcaaatctt gtacatcaag tgcgaggata
     2401 tcctccgcac gtttctcatc agccgctttc acgactagtt ctagtaattc tttttcgttc
     2461 atttaatcct ctttttagaa aatatgtttg taatctagga catctgcaaa gcgctcctcc
     2521 caaatgtcct cataaaattc attaatcgtt tctgctggaa tgtcatcccc atcaaaggtt
     2581 gtgacagcac cttctggaac aataagctga tagccatatt caaaggcaat cttgacagag
     2641 gtatccacac aatattctgt ctgcatacca cataaaacta atttttcaat cccctgctta
     2701 tccaagtatt cttttaagcc agtttctttg aaaatactgt tatatttctt ctgaaagacc
     2761 ttttcagcag gttttcgatt taaaagcgca gataactgcc aatcttctga tgtttgagct
     2821 tcagagttct caatatgttg aacatagata atttcaatat tcttgcttct agcctggttt
     2881 tgtaaataag aaattttttc caatagactt tttgtctgga acccagtttc tactaaaaca
     2941 ttctgaacat caatgattat aaaagccatc ttcatttagt tctctttcaa atagtgcaca
     3001 aaggcgttat aggtttcaag ggtttgggga tagatgggga atccctgatg agctagatac
     3061 tccacggtac gagctgtttc gtaggccacc gccttattga gcgatagact tgcaacctca
     3121 cgcgccacat ctactccagg aaaggatcga ttgtgctcga tatagtctgc tacatagaca
     3181 accttatcta ggtctgtcat ctgaccagca ccgactgtat ggacttcaat ggctcgcagg
     3241 atttctgaat catgcaaatc caaatcttcc tgaattttgt aaattccgac cataccatgc
     3301 cagacattat tgccccagtt tttgaggcca gggtctagct ggtaacggtc aatcaagtct
     3361 agaaattcct gatctgacaa ctttttagca tagtcatgaa gaagacctgc tagacttgct
     3421 ttctcaacat ctacaccaaa gcgctgagct aattccatag cggcacgctc cacacctaga
     3481 caatgggtta gacgtttctc aggtagaagc tctgccattt tttccaacaa ggcctcacgg
     3541 gagcagttga tatagtcttg ataggacatc agtagagccc ctccttctcg atgtagtcta
     3601 gcactggctg aggtaggaga aagttgggtt tccgaccttg ggcaatgaag tcccgcacca
     3661 tgctggacga gatatccatg agcggtacgt ccacccagat aactggatag gaagtccctg
     3721 ccttgtagcg tggacgctga acccccacaa actgaaccat gtcaaccagt tcatcaattc
     3781 ggtaccactt aggcagatag tcaaccatgt cggcgccgat gataaagtaa taatccgtat
     3841 ctggatgttg ctctgtcaag attttcatgg tgtcgtaggt gtaggaaata cccttgcgct
     3901 ccaactcaat ggtttcaatg actaggccgt caatcccctc aattgccaac tcaagcatct
     3961 tgagacgatg gtgttcaggg atggtttcct ttttatcaac gtgaggaggt tggtattcag
     4021 gcacgagaag aacttgatcc agtcccaact gttgccgtac ttgatccgca acaatgagat
     4081 gggcattgtg aacagggtta aaattccccc ctaaaatccc aacttgtttg cgttttttct
     4141 ccttgatttc tggctccaac tctaccttgg taaagggagt caatagttcg attgtcatag
     4201 gctagtcccc tttatttctc tataaaaaca gttatttgga gtttcggtct tagatttctt
     4261 tgactttctt agaaatcttg cgattttctt tcttgctaga ttgtttaaac aaaatcaaga
     4321 tgcgtcctat tttttggact gtatccacac cgatttcttc ttccaaaatt tcagctactt
     4381 cgtggatgtt ttcatctgtg ttttgtaaga gagtaacctt gattaattca cgcgcatcaa
     4441 gagcttgacg gacgctggtt ttgatttggt cgttgagtcc atttttcccg atttggatga
     4501 taggtttgag ggtgtgtgcc tggctgttga ggaaggcacg ttgttttgat gttaatgaca
     4561 taattttctt ccttgttttt tgatagttat tttaggtggt gagggacggt cggaactaat
     4621 tttccaaaga tttcatcttt gaaaaatgga tttcctgacc tcacaattga gccttggtct
     4681 caattgtgcc ccttgccaat ctacagattg gcaatcacac cacggcaata actatcgcta
     4741 tatgagctca ctttgttcgc tcagcgattt tgttttaaat aattgctttt cgtgtaacga
     4801 cggcgacgcc ttctggtgcc cagacggcga cttttgctgt gcctgttaca cgaatccagc
     4861 ctaggcctga gatgactagg tctgtcttat ctttaatggt aaagacatgc tggactagcc
     4921 ttgggaaatc ttctttttcc ttgctatttg gtggtgtcag aagagttccc aggtgcttat
     4981 cgtagaaagc actagctcct tcaagcttgc tacgatggag tttgagttca ttatcaaaga
     5041 aagcagtaaa tccttgcttt tctcctgcta taaagtcaaa gcgtcccaaa ccacctaaaa
     5101 atagggtttg ctcaggatta agctgatagg tcttaggctt gatttccttt ttaggactga
     5161 catacttgag gtttttggcc gtcaagtagt gagccatctg gtggcggtgg ataattcccg
     5221 gcgtatcgta aatataagat ccgtcgtcaa gcggaatctc tattttgtcc aaggttgtcc
     5281 ctgggaagcg tgaagtagtg atgacattct gatcacccgt gatttcttgg ataatagcat
     5341 tgattagagt tgattttcca acgttggtca caccgaccac atagacatcg cggcccttac
     5401 ggtagtgttc aatcttgtca atgacttcct taatggcatg tttattttgt gctgaagtta
     5461 ggaccacatc gactggacga agaccttctt cataggcacg tttcatgagc cactggctaa
     5521 tcttaccaga cttaactgac ttaggaagga tatctttttt atttcctacc aagaggacat
     5581 cattgcccga gacgaaacgt ggtaaacctg ggatgacaga tccattaaaa tcaaagatat
     5641 caatgacatt gaccactaaa gcatcactgt ctcccacctc gtgcaagagc ttgaggaaat
     5701 catcgttcgt caactggaca tctgtgattt cattgtagtg gcggagacgg aaacagcgtt
     5761 ggcaatagac ttcgccagtc tccaaacctt tttcaagtgc cgactggggg gtaaaaccaa
     5821 gaccagcttt atctgtcgtc tgaatggttg ctccacaacc aatacagaga atttcttcca
     5881 tagttaaatt ccttttttat atgtaatcgg tccgtacttt tcagtgattt ttctcataac
     5941 acgacgctca cgagttcggt taatctgcgt tttgattgag tcatgttgga ccaagggttt
     6001 gactaaaatt gaccgaatcc ctgcacggtg ggctgctcgt atatctgtca tgagctggtc
     6061 gccaaccatg actacttcct ttttgtcata gtggaattcc ttcatggcac ggtcaatacc
     6121 aaatgtgaag ggcttcaagg cccagtaaac gtaatcaatc ccaaatttct caactgctcg
     6181 ttgaacgcgt tttttggtgt tatttgacac tacgataatg ccaatacccg cgtcccgaag
     6241 gtcatgtagc cattgcttca tctctggcgt tccatcaggg ttgttccaag caatgagggt
     6301 attatccaaa tcgaccaaaa cagccttgat tccctgcgcc tgcaggcttg ggactgtcag
     6361 atcatagact gcttccacag caaaatctgg tatataattt tcaatcgcca ttctggctcc
     6421 ttttcttaac tttttttcgc aattttcctt agtaacaagg ataaaacaat ccacaaacct
     6481 gcactagcca aactgatata gagccaagca tggggctcat ctgttaaagg taagggaaca
     6541 ttcattccga aaaagcctgt cacgactgcc aaaacagcta gcaagactga aatgatagtc
     6601 aaggttgtca aattgtcatt cagattattg tttagaatat tgttgtaaga ggctgaaagc
     6661 tgctgtaaaa tctgagagat taggtctgtc atggatacca gctgatgagc ctcaatcatg
     6721 gcatcatcaa actgttctct ctcaatctca tcaaaactac gatacaaggc atgaccttga
     6781 atatgctcta acaaaatccg attttgtttg gcagctgccg tcagataaac cataccagtc
     6841 tccaaatcag acaggacaaa gaggtttttc ttggtagttc gctggcgcaa gaggtcattg
     6901 acctcatccc tactcttgtc catctgctca atgacaggat agtaggcatt gctgataatt
     6961 tccagactgg caaagagaaa cttataaatc gaaagcgtgt catggctctc cagataacga
     7021 gtcatctgtt caatgacata ggcgttcttg gtattactaa tggtaatcag gcgacgatgc
     7081 tcgacaataa aggtcatggg aaaagtctca tagtaggcct tgtccttttt tacatctagg
     7141 acattataga taaaggtaac cgtcccactt tcacggtggt agtccatgtg ggcgcgctcg
     7201 tttctatcca gtgcgtattc aatggtttcc ttgtccaaac cgtaaatttc agaaaggtct
     7261 tctagtttat tcaactttcc taggtctagg tctatccagg tacaaccatt gcccaactgc
     7321 ttttctaaaa ccatcttttc tccttattaa actccctcta ttgtaccaca aattacttaa
     7381 aataacaggt ctagtctgtg cgtgagaaat tgctaacaac tgtgatattt cggttaggga
     7441 caaagtgaag ggcttgctcc tcgctacgaa gctgtgtcgt acgaattccc acactgacaa
     7501 ccttacccga tacagtaatc ggtccatttg tcagaacgac ctcatctccc acatccagtt
     7561 gacgttcaaa gaggatgaaa aagccattga tgacatcaga cagaaagcct tgggctccca
     7621 taccaatcgc taccccagca ataccagctc cagccagcaa actagaaact ggcaaaccta
     7681 aaatcgacaa aatgcagtag agtaaaaaga aatatagcgt ataattaaac acattttcta
     7741 gtaaacgtga gatggttttt tggcgtccga catcatgacg agacattttt agagaaggtt
     7801 tgacaattct ctgcaccatg gtatgaagca tttttttagc aatataaaat acaatcaaaa
     7861 gcagtaaaag agaaatgacc ttggttagaa tattctcgat aatggttgtc acatctagct
     7921 tttcaatata agcctgaata aatttttgca tataaaactc ctttatttca ttataccata
     7981 ttttcatcag tctcattctc cataaatatt caaaaaatag acagaaaatt cttgattttg
     8041 tctatcattt atactataat cataatcact acacaaaaag gagattatta tgaaaagaat
     8101 cattcatgct tggaataagg caagccttgt caagcgtatc ttgattggta tgctttgtgg
     8161 ggaatcctag gactgaccct tcctaatctc tcagggattg ggctactcga ggatttattc
     8221 gttggaggtc taaaagctgt tgctcctatc ctagtctttg ctctcgttgc caatgccctt
     8281 tcccaacatc aaaagggaca agatagcaat atgaaaactg ttgttttctt gtatatccta
     8341 tgatagggac tttcgccgct gctcttgtag ctgtactagc aagtttcatc gtccctattg
     8401 aaattaccct aaatagtgcc aatactgaaa ttgcaccacc agatgggatt gggcaggtcc
     8461 tcagcaacct cttgctcaaa ctggttgaca acccagtcaa cgccctgctt actgctaact
     8521 atattggaat cttatcttgg gcagtcattt ttggaatcgc tatgagagaa gccagtaaaa
     8581 atagtaaaga attgctaaaa actatcgctg acgtgacttc taaaattgtc gaatggatca
     8641 tcaatctggc tccatttgga atccttggtc ttgtttttaa aaccatttct gacaagggag
     8701 tcggaagcct tgccaactac ggtattttat tggttccatt agtaacgact atgctttttg
     8761 ttgcccctgt ggtcaaccct ttgattgcct tcttctttat gagtatgaga cgcaatcctt
     8821 accctctagt ttggaactgc ctccgtgtca gcggtgtgac agcctttttc actcgtagtt
     8881 ctgcgactaa cattcctgtc aacatgaaac tctgccatga ccttggactc aacccagata
     8941 cctattctgt ttctatccca ctcggttcta ctatcaatat ggctggagta gcgattacca
     9001 ttaacgtttt gacccttgtt acagttaaca ctcttggaat tcctgttgac tttgccacag
     9061 cctttgtcct cagtgtggta gcagctatct cagcctgtgg tgcttcaggt attgccggag
     9121 gttccctcct tcttatccca gttgcttgta gccttttcgg tatttctaac gatattgcca
     9181 tacaagttgt tggggttggt tttgtgattg gtgtcatcca agactcatgt gaaacagccc
     9241 ttaactcttc tacagatgtc ctctttaccg ccgttgccga atacgcagca acccgtaaaa
     9301 aataactcat caaggcaagc ctgctcatgt cttgtctttt acgcttttat tctaacttat
     9361 taggaaattc ttatgtctat tagccaacgt acgaccaagc tcatcttagc tacctgtctt
     9421 gcctgcctgc ttgcttattt tctcaatctt tcgtcagcag tttcggctgg aattatcgct
     9481 ctcttgagcc tatctgatac gcgtagaagt actttaaaac tggctcgcaa tcgtcttttt
     9541 tctatgcttc tagctctggc tatcggtgtt ctagcttttc acttgagcgg atttcatatc
     9601 tggagtctcg gcctctatct ggccttctac gttcctttag cctacaagat gggctgggaa
     9661 attggcatca caccaagcac ggttttggtt agccatctct tggttcaaga gtcaacctct
     9721 ccagacctcc tagtcaacga attccttctc tttgctattg gtacaggatt tgccttgctt
     9781 gttaatctct atatgccttc acgagaagag gaaatccagc actaccacac gctggtggaa
     9841 gaaaagttaa aagatatcct ccagcgcttc aaatactatt tatccagagg agacggacgc
     9901 aaccgagcac agctggtagc agaattagac acgcttttga aagaagccct cagactggtc
     9961 tatttggatc actctgacca cctctttcac cagaccgact accatatcca ctactttgag
    10021 atgagacaac gacaaagtcg tatcctgaga aacatggccc aacagattaa cacttgtcac
    10081 cttgccgcca gtgaaagcct gatcttagcg caactctttt caaaaattgc aggtcaactg
    10141 agccagacca atcctgcttc tgatttgcta gatgaaattg aacgttatct ggaagtcttc
    10201 cggaaccgca gtctgcccaa gacaagagaa gaatttgaaa cccgcgccac ccttcttcaa
    10261 ctcctacgtg aagccaaaac cttcatccaa gtaaaagttg atttttacca aaaatataga
    10321 cagtaatact cttcaaacta cgtcagcttc accttgccgt atatatgtta ctgacttcgt
    10381 cagttctatc cacaacctca aaacagtgtt ttgagctgac ttcgtcagtt ctatctacaa
    10441 cctcaaaacg gtgttttgag cagcctgcgg ctagcttcct agtttgctct ttgattttca
    10501 ttgagtataa aaaagaaaac ttcagaccaa tccacaaaag gtgaatatct gaagttttct
    10561 tttatttata agtaatcacc ataaaggtta atagtagaac aaataaagcc aaactcacca
    10621 ataaagtgag caccttgacc aaggtgttgc tctgccagta gcttggttta ccaatattac
    10681 tagtggcatc catggaaaat agttgatttt gacggggttg tatagttacc aatacaatca
    10741 aagcaagaga taggataata gctaaaacaa ttagtatttc aatcatagat ctacctcaaa
    10801 attcccagca aagtaaagag caaaccaatc agaatcatca aacccagtaa aagtgaaaaa
    10861 gtcttactaa tcttttcccc tcgggtttct ttttccttct tgatgggttt ttgtttcaac
    10921 tcttccatgc ggccttcaac gtctttgtaa aataaatctt cctctgacat gttagcctcc
    10981 taaaacagtt tgcatggttt cctgatatct caccaagtca acataatttg catgttggcc
    11041 ttgtttgcca agtgcctgac gcatttgatc aacatctgaa agggccaatt taatggtctc
    11101 aaccaaagca gcaacttcac tactttcaaa tagatggtct ggagcgatat aaagtctatt
    11161 gtgcaccgtc tgattaaagc caagaatcaa gagattgtgc tcaaaggcct gacgcactgc
    11221 ctgtagtaac tcattactgt ggtttatatc caagtaaata tccgacagtt gatacagctc
    11281 ctgaatcttc tgtggactag cattctggta aaggaccaca ttaggatagc gaagcatgtc
    11341 taagagctta gaagacatct ctgtcaccgc tgcaatacgg aaagtgacat caggcaaggc
    11401 tcctgcgatt gcttctactt gctcaatctg atctgaattg gtcaagatta aggcatctcg
    11461 tcttaggaaa ttatcacgtt tgaactggta atgataaccc aagtgcacaa actgatcatg
    11521 gtatttctcg tcagttaact ctaaagcgcg ctcataagtc gccttatttg gaatgatgat
    11581 cttcttagta cgcacattat cactttccaa aatcaactgc atattacctg gaatggcatc
    11641 atagagaggt tcctgccata ccaagacatc cgagccagat ttatctggat gatggaagga
    11701 aaccaagaaa ggagtcgcta gagtattaaa gataagctga ctggtatcta tttccaaatc
    11761 ttgcaaaaag aaggtgataa attcaacttt atttgcaaag taacgcatgg actgaccagg
    11821 caaagtcaat aagatatcac ccgtcacatg gttttccagt aaaacttgtt ggccattgac
    11881 atcttggtaa actgtcataa tcggctcgct atctgcgcta taagtggttt tagcaaagca
    11941 agcaccaaag cgattgtagt ggtcaacctg acgtactcga ccttctaggt ctttccagtc
    12001 tacctgttta accaagcgag cctgcattcc atcaacataa tgaatgacag ccctctcctg
    12061 cgtcacatct tcaatacaag cagactgatt atctcctaaa atttcccaaa aatctgaaac
    12121 gggaacttgg ttaaaataga gaggttttcc atcctcgtaa cctagataat aggtaaaagg
    12181 agaaagcaga ccatcaggca gaaaaccatc tgcatcgatg accactccaa gttgagaaag
    12241 accagtagcg actagacttt catgtaaatc tcgactttca tgactataac gatcataaag
    12301 ttcaatcatg gagcacctcc tctactgttt tcttccactt ttctaaaatt tctttggtca
    12361 agaagccttc tgcaatttgg taagaatagg cacgcatagc ttccaaacga ttttcttgat
    12421 acaattgaca aatcttagcg gcataagctt gcttgatttg gtcttctaca tggtcagatg
    12481 aacttggaat caaataacca ttttgcccat cctctataaa ggtctgatta ccataaggca
    12541 catcaaaacc aattagaggt agacctgaac caatagcttc catcaaggtc agaccaaatc
    12601 cttcgctggt agaagccgtt aagtagacct catactggct ataaatctgc gaaagttccg
    12661 catgcccctt gagttggata tagtcctctg cctgatgatt tgcaataatg tctctaagca
    12721 gagaatcttc tccaccacta ccatagatat caaaggttag ttccggtaac tccttatgag
    12781 cttcaatcac agctttcaca agccaatcaa tgtgcttttc tttggcaaga cgtgaagccg
    12841 taatcaatga aaatggtttg cgcccttgac ttgaatctgt caaggaatca atactgccta
    12901 caggaatggt aacaatcttt ggctgatgct gagtatattt ggcaaattgc tcttgtagaa
    12961 cttcattttg tctatcagta gacacgataa agaagtcaac cttatctgca ttggtaaact
    13021 gatagtcata atagttattc caaaggatgt agtcctcatt tgtagcattt tcactataat
    13081 gctccgcatg aacaactacc gctaaatgtg ctgtctgtac ttcctcaaac acaacctgac
    13141 caatgcctgt ctccctatcg agaatgacca aatcagactt attcaaattc aaagatttca
    13201 taaaggcacg cacaaaagct tgctttccat agaaaatctt atccttgaaa tgataaactt
    13261 cttccttccc ttgattcatc aagatatcat agactggagt cccgtcttca ttataaaaag
    13321 ttcgttggta taagactgca acattgtcct tgggagcaaa atactcgcta caataacgcg
    13381 tataagaaaa gtaatccttc cgaatcaggt ttcccttaaa aacatactcg gcatgttgaa
    13441 ccaagtcctt gttctcatca accaaataac aggttacaaa cttatcttgg tcaaaaaaga
    13501 atacacgtaa aaccttgcca tttttttctc tgtgactttc ttcaccacca aagtaagcca
    13561 agacatcatc cactgtcacg ctagtaggtg caattttgat atctgtgaaa tgattataaa
    13621 gccagataac ctgattatca tcaaaaccaa tattggctgt taagtgctga atattatcgg
    13681 ctaaaatcat atctgtaaag ataaacttag aggacagatt taatttccga aaaacaccag
    13741 cacgataggc ttgagcgtat tcaacaccgc tactagccca accaattcct aaatttatat
    13801 tgtaaattgt catattttct cctcttatgg aaaatgagtc acgatttcac cttttggagt
    13861 gacctcaacc agccccatca ggagattacg caccatatcc gcacggattt gttctttcat
    13921 agcttcaaag cccgcgtagg cttcttgata gtactctacg attggatttt tctgactagc
    13981 agattgacca ccgatagcca tggatagctg ttgtagatag tctacctgct ctacccagtt
    14041 gtcatcaatg gctttaagca gtgaaagtcg taaaaactgt tcatataagt catgttgatt
    14101 aagtaattct ttcttttcag aaagttcttt atcaatcacc tgcttcataa agctacgaac
    14161 tgcagttttg tcagttacat ctatataatc tggaacctct ttaacatgaa aactaatatt
    14221 ggtcacaata aagtgaaaca ataattcacg actagcatag tgatcagccg ctacctcttc
    14281 tgtatatctc tcaatgatat ccacaacaac atcctctaag tcacgagaac catctattag
    14341 acgatttctc tctttataga ctatatcccg ttgaatattc atactttcag catattctag
    14401 agtctgacga cgtgccgaac ggccagcact atcactggca tgctgagcct tttcgactag
    14461 tttccggtat ttacgacctt tcaatacttc cggttgagtc atatcttgaa cctgatagtc
    14521 tttgtacttt ttatgcaccc aagatggacc aaatttcttg ataacatcat cctctaagga
    14581 tacaaaaaat ttactcatac caggatctcc ctgacgacca gaacggccac gaatttgtag
    14641 gtcgatccgc tgactttcca tccgctcagt cccaataaca atcaagcccc caagctctgc
    14701 gactccttta ccaagcttga tatccgtacc acgtcctgcc atagaggtag ccactgtcac
    14761 agcccccatc tgacctgact cggagataat ctgagcctca cgcgccgcat tattagcatt
    14821 taggacatta tgggcaatcc cttcacgaaa caagagagac gaatagagtt gagacatttc
    14881 aactgagcct acaaaaacga gtaagggatt tcccttagca tggtattgct tgatgtactc
    14941 caaggatgca tacacttttt caggtaaagt gatatataga ttatctggat agtcaatccg
    15001 ttgtctcgga cgattggttg gaatgcgtac tacagacata ttgtaagttt caataaactc
    15061 tttttccgcg accttacctg tccctgtcat accagatatc ttattaaaca tcttaaaaag
    15121 actctgatag gtgatcgagg ccatagcccg cgtctcagga gataatttga catgttcctt
    15181 ggcttcaata gcctgatgga gacctccttg aagtttagtc atttccatta gacgccctgt
    15241 tcccttatca accagtacca tctcatttcc acgaatgata tagtccttat ctttagtaaa
    15301 gagcttatga gctcgaatcg cataaaccaa atgacgagca aaagacgcat gctcttcctt
    15361 gtataaatta tcaatcccta ggaaattctc agcagacttg gcccccttag tagtgagcca
    15421 aacctcctct ttctcctctt taaagatata atcctctcct tcgaccaagg ttgttacaag
    15481 tgtatcaatg atcgcatagt aattagactg aacacgagga gaacccgcaa taatcagagg
    15541 agtttgtgca ctatcaagca agatatcatc aatttcatca ataatcacat agttaaacgg
    15601 tcgtaaaaac ttaccttctt cattcgaggc taggttatca tttagataat caaaacctaa
    15661 attactattg gttgtgtaga tgatatccga agcatagata agcttctttt cttcagcttt
    15721 catctccttc tttggatctt ccgtaaatgg tacaccaatg gtcaatccta gaaaacgata
    15781 aacttgaccc atttcctcgg catcacgctt tgataaatac tcattaggag tcacaaccat
    15841 cactccttct cctgaaaaag cgttcaaata gacaggcatg gtagctgtca aggtcttacc
    15901 ttcccccgta ttcatctcag caacatttcc atagtgcatg acaatagctc ccatgacttg
    15961 aacatcataa ggaaacatcc ctaaaatccg cttatctgct tcacgcacca cagcaaaagc
    16021 ttcaaccaaa atatcgtcta gactttctcc ctcggaaaga cgctgacgaa actctactgt
    16081 tttagctact aattcttgat ccgaaagaga ggacatcttg cctttcaggg cattaatctg
    16141 ctttaaaatc tttttcactt tcctaagctg gaaatcttga cctaaacgtc taaacacttc
    16201 tgatctcctt gatagaaaaa tgataaaaag ttaaggactc aaatccagca cttaaaagag
    16261 aaacttgata agtataacta tcttctggat aagtaaatat gaaatctttc actttttcta
    16321 cctttttttc taaaatatca ccatagcggt caaaaaaagt tatcttggta taaactccat
    16381 tttcaggctg acaatcaaaa accagtgata gttgatactc ctgctttctt tttaataagg
    16441 gaagctgagg ttgggttcgt gcagcctggt aattgacact agaaaaccaa gtctttagaa
    16501 tctcaccaga tggcaataga ggattataca gacgaacact tttgtcaggg taataagata
    16561 cagttgttcc atacatataa gtcccaccaa cttctcccca atgaatagac tgtctctgtg
    16621 taataatcat cttttctccc ttccaaaacc agtctctaag accattctat aaaagtgcaa
    16681 aaaccaagaa atattggtat ctgtatcatc attatgtcgt ccagcagttc ctttagataa
    16741 aatcttcgca cctgtattac acagataaga cactaattgt tcataggctt gtggatccat
    16801 ttcttcatct ttcatatagg ataaaccaaa ggttgtcttt gaaaaatttg cttgtttaaa
    16861 aattttccaa aaacgctgat ccaactccgt catatcttga gaacttgtcc cacctgtatg
    16921 atgaattaaa caatcaaaag ctaagttaga gacacccggt gcgtccaaac gtccacgact
    16981 agctatagtt cctaaattag ccaagggctt accaactatg atggcatggg gttcaaaaga
    17041 agcaccataa tagagagccg gaaacgttcc catagaaaga cctgataaaa tcaaatcctt
    17101 atgatcaaag cctaaataat caagatagtg agtgatcgta tcctttactt ttccctctag
    17161 ctcatccgtt ccgagataaa aagctccccc ctctaaacgt ggatcagaaa ataaaatgaa
    17221 gggacatcca agagtcttca tcataaagta accctcgaat ccttctgcag gtcgataacc
    17281 tgcaaaatag acagtcaaag gcggtttaaa atcacctgga tggaagaaat agtttatttc
    17341 atcacgcttg gaatcatgta ggatatttcc accaagtaca aacttaccaa attgttttcg
    17401 actccatcgt tggtgaagat tacctagttt gatagttcca cgacctcttg cttcaacaga
    17461 tatcgagaga aagtagtctc cgtctcgact atccataaaa agtgcttgct ccaagtcttt
    17521 ttcagatact attacttctt caaagaaatc atcaacagac ccactccaca ttttacggat
    17581 tactaaacga aagtcacaat tgccttcctt ctcatattca agccacaact caatagggag
    17641 tgttttttgc accataatat tataagccca ataggcaagc tgggcaaaat cttgcccaaa
    17701 ctcaccttcc aaactgacat aatccaatcc ttgataagaa atagctcctg taaaatttgg
    17761 attgacttgt atcgtcggag gaaagagttt atcaccatac ccaccgccaa aaagagattt
    17821 acttaaatca ttgattagtt gttggggact taaaaaatca attgcttgtg cacatcgttt
    17881 ttttagaaaa tccacaacct ctctatcatt tatttgtaga ttctggttat aaaataaagt
    17941 ataaggttca aaatatttta cataggaaaa taagtctctg gaatactgac catcttctat
    18001 taaaactgca tgaaaacggt taatatcctc tttttcaatt atttcacgaa gggcttttga
    18061 tgagtttggg taaaaataat accaactcat attttcaggt atgtcatagt gattttccca
    18121 attggcaagt cctatctgta aaatattaag ttcctttgac atttttaacc tcctctatcc
    18181 atttctctaa ttttccaaga aattgttgtc ctgtgtgctc cttaatctta tcaattgaat
    18241 atatcaagga ctcattccac tccttcaacc tatctgtgta ataatgtgca gccttagaaa
    18301 attctgtaac atctgctaac aaataaccat tttttaaatg ttcaacatag acggtttcaa
    18361 ctaggttgat ttgaggaatt cctgcactaa tcccagcaat ctgtgtgtag agatgaggat
    18421 gtctatttaa atccacaatc aaacggacaa attctagtgt ttttatcaaa tctaactcat
    18481 cattcaagtt aacaaactgt aagcgtaagt cctggtgttg attttcttcc agaggatttt
    18541 ctgcatcacc ataatcaatc gcctttccca gattttcgga ttgaatgttt tcttggatga
    18601 aagactcaac aatcccttca acctcattca tttgctcctg actagcagca aaggctccaa
    18661 aaatcacctc agtatcctta ttttcagcga caaaggacaa gacttgaagc agagcttgat
    18721 tatcaatccc ctgctcaaaa tccagttgaa aataaatgat ggattctttc ttagtttggc
    18781 ttcgtcccaa tcgtaatcgg gtgtcaaaag atgataaatg ataacattga tgcattcgtt
    18841 caggatacaa ctcctgcaac aatcgtaaac tatcctctct atccaccaaa atcaaatccg
    18901 atttttcaaa agttacatct aaatccctaa aggtatcttg aggattacgt ccaatgaaca
    18961 gactcaacag tttaggatta gtactaggta aacgatctag taccaactgg tcatgatgag
    19021 aatgagaagg aagcataaaa atatcctgat ccttcacata cgtttgcaga taattctcaa
    19081 aaaattctgc caccagatct cccatatttt gataggtaag cattttaaag cgataaccca
    19141 aaattggatt gacttctacc tgtcctcctt cttttaaacg ctctctaaat tgccaaactc
    19201 ccttaggatt caaatattct tgataggctg cttgcccatc ttcaaaaaaa ataatactcg
    19261 atataaaccc tcgatcatcc atgatatggt gcatatgtat ttgaccattc ggtccaaaat
    19321 actgtatatc actgatgaaa ccttcaactc catgttcaac cttagcaaat ttcttcccat
    19381 ttttttgaac gataatcgta aagggactat aaataaattc acagtcatca tcccactcaa
    19441 tatctctaat gcttaaaact tgggtatgga gattatgaaa atcttgcata acatcaaaaa
    19501 cggaataagt atccgtttct aacacaccat gtctatataa aaaataacgt agatgaggct
    19561 ggtaagctaa tactaataga cgagaatcta tatcttgctc ttgaaagagc cgaatctggt
    19621 gaaaggtatc atcaaactct agacgaaaat gagaaaaata ccatggagtg atatctgcat
    19681 gccatgttct ttctgaccca taccaagctg gaataaaata atacataaaa gtctcctaaa
    19741 tcaaaggtct ataggtctca tttagcctta aagccttgac ctcgtctcta atcgtaaaaa
    19801 tcatgccccc gaacatcata aataagcctg gaatcattgc caatcgtaat aacttttcat
    19861 caaacaaaac aaaaagcatg ggaccacctg ccataatcac attaaagaga ccacctatga
    19921 ctgagaaacg aaggaccaat cgattaataa atcgactagt atccgcacct ggataaatac
    19981 cataaatgta ttctccagat tttttcatac ggtctgcaat ctcttctcca ttcatcgtaa
    20041 caaaagcaaa aatgatacta aataaaaata agaccgaaat ataaacatag acccacaaag
    20101 gcttcccaac cataaattcc tttgataaag ccgctagccc tgaatgatta gggaaaataa
    20161 atcccaacaa gatgaacaaa taagctggta cactaagaaa actcatcaca tacatataag
    20221 gcatcccacc tgcaggattc aacatgattt cgagataaga atagcgttta aatcgattgt
    20281 gtaagccaat tttattaaca ggaaccaaat agcgagctcg atacataagg gcaagcaagt
    20341 aagaaaatat aatggtcata aaaacaagca agacaacaat ccctgtaggg atataaacca
    20401 gtttaaaagt ttcaataaca tcctgaggga tatttaaaac aatgctggat aggaggatta
    20461 caatagaacc cccaattccc atgctagcat ttaaatctga caaccaaaca agaaaaaacg
    20521 ttcctgctat gagtaacaag gtattcatta gagcaaccaa aattaccgag taggaggatt
    20581 gtactggcaa gctcaagcta actgccaagg attgaatcac agcaattagc aaggtcagat
    20641 acattcttcg acgatcttga atttctatag acgtagatgt taaacccaac cgtttagaaa
    20701 aagaaaacat ctgccataaa atcatggcgg acatccaagg ggataatcca acagaaaaaa
    20761 ttgataaact tcttagattc ccacctgtta gggcggctga gaaggctaga taggctgttg
    20821 aacctcctaa aaaatcttta gtatttaggt caacaaaagg gagagtaata cgacttccca
    20881 atacatagat aaacagtaga aataaggtaa atagtccttt ttttactgct attgacgaat
    20941 aaatcttagt cataaaacac tcccctcacc ttcattgttt gtgatatggt aaataacaat
    21001 tcttttattt caacttttac catttctttg ttctgcatca aaaaacaact ttaatctgaa
    21061 acaaatatct ttatattcct cagtatctgt taattgatgg tattctaaag tttgcttata
    21121 atcttttaaa agattgacaa atcgaatacg cagaacggag acatccaaac cagccaagac
    21181 acaatccgat attttcttag aaaacacttc aacaatatct cttgcccagg aaagatcaaa
    21241 ttccgttctc atgatgcttc ctgaacgtct tctatacaga tagagattat cgtttataaa
    21301 gacgatttta gaggctaaaa gataaaagcg atgcatagtt gcttcatcat caaagcggcg
    21361 accatttgaa aagtgaactt cattgaataa ttctttttta atcaacttaa atgtcggcaa
    21421 tataaaggcc gagctattga atttccaatc tcctgcttga cggttcataa tttcttgtat
    21481 agctaattct tcgacgcaaa aatctttccc tgttacataa aaataaaaat tcccgtcact
    21541 ttcgtcataa ttatagtaat tccctataac aacatctgca tccttttctt ggacttgctg
    21601 ataaagagtt tcaatcatat tttcttttac gatatcatct gaatcaacaa tcgtgatgta
    21661 ctctccctta gctacactaa tccccatatt tttagcttgt gctgcccccc ccccttattt
    21721 tcttgatgca atactcgcac acgattatct tgcatcataa aagcatcaca aatttcccca
    21781 gaattatccg tagaaccgtc atcaactaaa ataatttcta tattttgata ggtttgcttc
    21841 agaatatgct cgacacactc cactaaatat tttcccgtat tgtatatcgg taccacaaca
    21901 ctaattaatt cactcattac tctatctcta tattcttcat tctatccact aaatcatctg
    21961 gtctctccac agaaaaaata ccgtcataga gtccatcatc catactttta cgtgtgatat
    22021 caaaagcgaa gattttcttg ccactttctt gtgcccttgt aactacatta aaaacctctc
    22081 cacctgtatt aatatctaaa taaactatag aatcgtccaa tagccaatca attctactat
    22141 gtaatacatt ttgatatact gttacattcg tatactgtga tagagaggtc agctcctcag
    22201 aacaatcaca cggtgcagcc aaatgaaaat gccaatcagg taaccgttgt actaaatact
    22261 ctaaatgttt tatatctgca ctccatgtca caagcatcac ttgtttttta gactgattgc
    22321 ttctttcaaa ataatttaaa tcggaacgtt gaaaagcaat ctctgaccaa tccaaatctc
    22381 tataaaccca ccataattca cgtagtctag atatactata tgtattccaa ggtttatcat
    22441 gactagcata gtgaacaatt gtaggtggat tgtcatctag acgttcacat tcttgagcaa
    22501 ggtgataaat atcaacacct accatataat tatatgtttt atctaaggct agccaattat
    22561 cctcaaaata aatatttaaa atactctgat ccccaagatg aacaacttga ttttgctcgg
    22621 ccgctaattc caataaacta ttgacaatag agtgttcttt ccattttgca acatccatta
    22681 ataaaactcc agaattaaat ccagattttc gtccttcata ggcatagaca tcatcaacag
    22741 caccaattga atatcctttg agatctatct caaacaaagt agctagttcc ccagttacaa
    22801 tgatatcgga atccagatac aataccctat cagattcaac cacttctgtc gcaaaaaatc
    22861 taaagtaaga agcataattt atatgaggtc ctgttttata gctttcaaaa agttctttat
    22921 caatgtgtac actgcgaatt gtagaattca caacttccat ttttctcccc aataatttaa
    22981 accattcagg agctatatca ctgttgagaa tataaaaatc aacatctcta ttgtgataca
    23041 atacagactt tatagtcgtc tctaaaggaa taagatagac attatctgcc gctaatacta
    23101 ttgattttct catactgttc tctacctcat gtaagatctg attgctgcaa ccattttttt
    23161 cggaatggag cttggataga taccttcata tgaaatctca gaaagatttt cagagcaagt
    23221 attgtcgaaa gctatcattg gttttttgta cttaagcaca aactcatatg catcttctaa
    23281 ttttcgatca tgattcaaat ccaaatacaa atctgaagct attactattt gttccaataa
    23341 cattggtaag gaatttggat atacggtaac attaggaaaa cgcgttaaag ctagtagttc
    23401 atttgccata tttgtataag caacaatatg aaaatgaact tctggcaatt gctccgccaa
    23461 ataatcgatt ttctcgacac accaagaatt cgttaaatta acacattgca acttaaatat
    23521 ttgtgattta ctaggatact tcacactctt tgaaaaccat tcatttaaaa taacagacca
    23581 atccatcaaa gagtattccc accaaacttc tcttagacgt ccaacagaaa attgattcca
    23641 aggcttatcc tgagaaatat agtgtaaaat tagtggcagt ggttcgagcg gaatatcaaa
    23701 aatgaattga tgtttaaagg ctgccgcccc ataatcatat cctatttgaa aattatattg
    23761 atcttcaagg gaactatatt gatctttaaa caacatattc aaaattgact ggtctccttc
    23821 ttccacattc tcatgttctt tttctgttaa gtcaatcaat ttttgtcgaa tagtttcaga
    23881 tccccatttt ttgttgttaa tcaagagaac accagcattg aagccgactc ctgctccaaa
    23941 gcaagaacga gctgctgcca aataattttc acctaagtct aattcaaaca aatcggtcaa
    24001 atcaccagtc acaatcaaat cactatctag atagagaact ttatcttctg ttacaaaatc
    24061 tggaataaaa tagcgtgcaa atgtcatatg attgatatga ggtaatttat tagaccaatt
    24121 catttgaaac tgtgagccaa ttaacttgca gtcaatcaag tcgcccccca tctcttgtaa
    24181 atatattctt atttgactaa accattcctg aggaatgtcc tgatttagca gataaatttt
    24241 caaatgacta ttgtgtctac agagtgactt catcgccgtt tcgatttgtc gaatataagc
    24301 gtaatcacct gcaaatacta cagatttctt atcttgtaac attttatcgt ctctctatct
    24361 taattttcag ttatatttat taccattcaa taaatagtct aacatttcta aatgtcttgt
    24421 ttccacattc tccttaagtt ctcgttgaat tgcaatatca tgacgatatt tcaaatagaa
    24481 cttcatctgg tctaaatatt tactgacatc atatcctttt agcgctagca cggcaattcg
    24541 ttctaaccta aaatcacaat catcacgcat catcttcggt gaataagaat tagaaagtcc
    24601 ttctttccca accctatacc aatacaaaac tttattaata taggttactt tctcaacctg
    24661 cataaataat ttataattgg ttcctatatc ctcacacacc cgaccaaacg gaaactgaac
    24721 ttcttgaaat aattcccttt taaatagaat tccccaagaa gttaaaaagc tcatatcata
    24781 cctctctaat aaaggaagtt cttccattag aagttttgaa ttataatttt tcacataata
    24841 atcggagtca aatacgtgaa tatagaataa gccatctgac tcattatagg ttgaataagt
    24901 tgaaacaaca atttcagaat gctgctcctt caaagtttct ataagaattg ataaatactc
    24961 ttcagtaacc caatcatctg agtctacaaa agtaatataa tctcctttag atctctcgat
    25021 tccaaaatta cgtgcatccg ctaccccagc attttctttt tcgaaatagt gaaaacgact
    25081 atcacgatca gcatactctc gacatattgc tcctgaatta tctgtagagc catcatttac
    25141 taataaaact tcaaaatttt gataaatctg tttcgataaa ctgtctaagc agctagaaat
    25201 aaatggttct acgttataaa ctggtactat aacacttatt aaatctttca tacttctctc
    25261 tttgataaat caactaaaat ctgactgtta taggtttcat cctcaatgaa ctgaatatct
    25321 tgatcaaaat tatactctgc tgtatgttta tgtgaaaatt taaccttagt tttagcaccc
    25381 atcttcatcc actcatactc ggagtcaata tgcccaatat ctaagacttg gtaacccttt
    25441 cgacttaaat gataggctaa aacttttgct gtaggaccca gcatacataa aatcagtctt
    25501 ccttcagcat gtttcataat ttcctcttga atgttatcga caacggaata agcattatga
    25561 gatggacaaa taatccgttt cacagatttg accttatcaa ataaatcatt tccaacccca
    25621 gagcgagatg taatcccctc tacaatcaga atatccctat tctcccaaat actctttagc
    25681 ttttcaaatt gtccttttgc cttactctta tcttcataat caatgtatgg acgcgagata
    25741 aaggtagagc cataccatga acccttacac aactctttat aaaaatccat ataaacattc
    25801 atatgttctt tccagaatgg tgtagcccac caagtaaaga caaaacgatc gtcaaaagca
    25861 tcaggtaaac aaaccactaa tttctcgtca ctctcaagac ccacaatatg ttttaattgg
    25921 ttagctaact ctagatcatg attttgataa ggtataggca aaccccacat caaattgact
    25981 tctccgtctc caaaacgaac taaagaagag ttgtgttcaa taatataatc caaggtttca
    26041 tctatccctt tcacctggat tatatcaagc ggtttttcta gcatatcctt agttatcaat
    26101 ttttgcaaat cagccaacat tcgttcagga tgttcacttg aatacaacct ttggccatta
    26161 ttcccatttt gagatttata aaaggctagt ataggtttag atagatgagc catttctgca
    26221 acgatatcgg atgttgaagt cagtaggttg atatctaaat aagcatccat cttctccttc
    26281 aactcgtcta gcttatctct agaaattgcc ggatagagct gaatattagg ataacgatta
    26341 gacaaatcat ataattttgg ccccatagct gtccaagcag caacttgaaa aacaacattt
    26401 ggcaactgtc cagccaagta atccaattgt tcgatatcct gagaagccgt aaacgttaaa
    26461 caagttaaac ttggaagttg tcgaaaacag gtaagatgtc cgtttttctt aaaatcttta
    26521 atcgtatcaa tcataaagga aactttccca ttttcgaaaa cgatttgtcc taactctccg
    26581 tgctgagtcg attgaaaagc tagtagcgtt ttttcttgtt cttgcaaaga ttttaaaaag
    26641 ttctcatctg cactaccatg attgatatcc aaatatagat ttgttgactt tttcagctta
    26701 tctaatacag gaggaacaat ttgtggatgc aatctcacat tattatatac agctaatttt
    26761 tttaatttat ctcccatatc cgtccaagct gcgatatgaa ataccacctc aggtagagct
    26821 gtaactagct cttctattcc ttctaaatct tgggaattcg ttaaggttaa gcaagaaaat
    26881 tccttatcta gaggcgatat tagttcaaat tctcccaaat gatgttgtaa aatctgattc
    26941 cactccaaat catggaattc ccaccataaa tcacgataac gattggctgt caaggtggtc
    27001 cagggttttc tgtaggtcgt aaaatgaatc accactggtt tatcattaaa agccagatgt
    27061 tcctgccaat tgttatacaa agccacaata tcatgcccta cttgtaaatt ataagctcga
    27121 cctagttcta accaatcatc ttgtaagacc tgattaaaaa tcgtttgatc accattaaaa
    27181 tgctcgaaac ggccttcttc aacttccttc attgtaacaa tgctctgttc aattagtcgt
    27241 tcttttaatt tctcttgacg ccatttttca ttgttgatta gtagaacacc agtattaaaa
    27301 ccttgaccat ttgcatctct aatcgctgcg atataatgtt ctcttacatc ctgctcaaat
    27361 agactatcta atttatcatt gataagcaag tcacaatcta aatacaaaac cgtatcttct
    27421 tgtatataat ctgggataaa atacctagca taggcaattg aactaatgtg agcctgtttt
    27481 ttccaatctt gaaacacagt ttgttcaggt agtttaacat cgataatctc actacctaac
    27541 atgctagcta tttttcgtgg ctttcgaaac caatctggca tgatatcttg atttaaaata
    27601 taaatcttaa catctcgatt gtggtataaa atagatttta tggttgtttc taactgcctg
    27661 gtgtaattac gattaccagc taatacaatt gttttaatca tcttgttctc ttattcaact
    27721 cccaaatggt aaattgcatc taccaataat tttttagtaa aataaccttc ttttagcaaa
    27781 taactaaagg tttttatccg attcgtcatt tcttgatatt cttgtagatt cattttatca
    27841 actatctcat gaacttcctc tagactattc gccacaaagc ccaagccttg atctactata
    27901 aatttagcag ttgataagtt gctcggtaca atcactggga taccagctgt tagataggta
    27961 ctaaccttat gagatatatt caaggtatag tattggttac tttctccctc attttgatgg
    28021 gttccccaga caaggccaaa tccacccttt gataattcta gcaacaattc ctcatctttt
    28081 ttccatcctt cgatgctgag atttctagca ctagaaccgg cctccccttt atttgaaaat
    28141 actctcaaag gcgtatcttg agaccaattt tgtaagtctg gaaaacgctc taaacttcca
    28201 gcaaaaaaag ttcttttttt aaaagcaggg gtgtataagg ataaatcatg aggatgatcc
    28261 cacatccctt gaacaagaat cttcttagtc gtcaatcctt cttccatcag gcgtgttttc
    28321 attctctctg acggcactat caaaacatct gatagattat acatgtacat atagtctttc
    28381 atgagataat agttactatc aaacatgagg ggaacaacat catggataaa gcaaataatt
    28441 ttcacctgca tatcctttag cttatcaaac aagagacgat caaattcaaa gccattccag
    28501 gttggagact gaaagactaa gatatcacca atagagatac tggccataat accatccaga
    28561 cgcttattca tttcagaagg actatctgaa gcaatgttgt aaaaataaat accaacctct
    28621 ctaaatccca gttgactagc tatcttttga acagcatttt gagctaagat aaccgtacta
    28681 tcaccagcca tgccgtataa atttgttaaa tgtaatttca tattacgcta tctcattttt
    28741 tattactatc tacggaagta aagacaattt ttccatcatt cttctataaa tctctgtgaa
    28801 ctgcatattt tgttcttcta attttgctac tactctttgt aatctatttt tataaatcaa
    28861 aatatgattg gtcaagtcat agtctgaact tgctaaaata gctatctttt cttccctagc
    28921 cccaatttca tcatacatac gcttttcact ccatgtattc gataaagtat cagataaacc
    28981 aacacggtac cagtaaacac aacgattcaa atagactatc ctcgaagctc ttagaagtac
    29041 cttccatgtc cagtaagtat cctctgcaat ttttcctata ggaaatggta aatcttgtag
    29101 taactctctc ttgaataact tcaagacagc tacagtccag ttcccatttc tgacttcttc
    29161 gacaccttcc ctatccataa ttgctttacc ttctatcact tctagagaat catctggatc
    29221 cgtaacataa gtcatataca catagcgtgt ttcatcataa gaattataac gcccgatact
    29281 aatatctgcg ttttcctttt tcaaagcacc atataatcgg tctaaagcat catgttccaa
    29341 ccaatcatca gagtctacaa aagtaatgta cgcccccccc gaacattcaa tacctaggtt
    29401 acgagctgat gaaagaccgc cgtttgcttt ctcaaaatat ttgaaacgag aatctttctc
    29461 tacaaattct tcacatattt tggatgaatg atctggagag ccatcattga ttaataaaca
    29521 ctcaaaattt tgatacgtct gattctgaat gctatccaaa cacattctca gataattttc
    29581 cacattatat attggaacaa caatactgat taactcattc tccattgact tattctccta
    29641 aaaatttgcg aatagcatct accatcttat ctggctcagt tgctgaaaaa atccgacttc
    29701 ttcctttgct atcatgacta gtattatcaa aactaaagat tggcttattg cgcctacaga
    29761 cttcttctat aatgttgaca atctctcctt catggttaat atcaagatag aaatccatct
    29821 tatccaaggt ttctttcata ttaaaagaat taaaatttgg aaaaagtgaa agattcagat
    29881 atctctgtaa atcaacaaca gagggagcaa aatttgtatg cgccaagatc gtaaagtgca
    29941 cttctggcaa ttccgatagg agatattcca aggactccat tgcggcacta tttgtaaaaa
    30001 tacaagtatg atataaggga acacccacta actccttaaa agagcgttta acgatatctg
    30061 aacttaacaa cacatcgttc cagttcaatc cataataaaa ccaccagagt ttacgaaaac
    30121 gtgtaagtgt taaatgagtc cacggtttga actctgtagt ataatggaca atcttaggct
    30181 cgtaatttcc ataatagttg gattggtacc agtcagcatt accttttaaa tgtaaaaact
    30241 gatctaaacc aaccatgaaa ttatactctt tgtccaaatg tagccactgg tcaccgaaat
    30301 atatattgag aacaccttgg tccccataga catgttcatg gtgttgagca gtcaactcta
    30361 gcaaattttg tgttgatttt ttttctcgcc atttttttgc atcaatcaat aacatacctg
    30421 aattaaatgt tgttaaccaa ccatcttgat caaaatcttg aacagctgcg atagcgtatc
    30481 cttgcaaatc caaaaaaaat aattcatcca aattggcatt cacaatgata tccgagtcta
    30541 agtagagtac ccgatcttca agcacttcgt ctgctatgaa atacctaaag aaagttgcat
    30601 aggacaggat tgcatttggc aagtggaaat cacgaagatt gtgatctgct tttttgacat
    30661 taataatctc agaaccaata accttcagac gatttctcat caaaagaaac cattcactag
    30721 gcagatcatc attaaacaca taaaatttca cttcgtgatt atgaactgaa actgacttta
    30781 taacggtttc taccttatca agataatgaa tatcagctcc taaagcaata acttttctat
    30841 acattcttat ctcccgattt ttctatttta taaacaaatc atctatttta ccaactgaaa
    30901 aaacgtgatt tctacttttg agatattgat tttttacttc tgtttcatca acaattacag
    30961 tatccttttc ttcgatgata tcttttcatt tctaaatcta aatgttgaat caaaaaatct
    31021 tcaaatcaat tatgctatga ataagatttc tttcgtgttc tgcaaaattt acaactatct
    31081 acttatagat tcggcattat caaatagatt aaagactcac cattaagata tcctctgaag
    31141 tcatgtttgg ttttcttcca accttagaaa gtttcatcta acttgatttt atattactca
    31201 ttaaactctc atctttacaa aaagatttcc attattataa actacactgt ttgatggaat
    31261 atcattgcgt atgagacaac ctgctccaat aatggtatta tctccaatcg tcactccttt
    31321 taaaatcgtg acattacttc ctatccagca gtctctgcca actctaatcg gcgctgtagt
    31381 ccactgccac ttttcaatct tttcagcggt ataaatatgg tcgtgatcat aaaaacgaac
    31441 accctctccc atcatggtac cagctccgat ttcaattctt tccatacagt taaaactaca
    31501 agagttgttg ataaaaacac cattattcag gattaactta ccagaagaga catgaaaatt
    31561 ttcaaaattt ctacaggcga cattggctcc tacaatgaag tgagccttat catcgatttt
    31621 aaagacattc caagtcccat cttgaatgat tttctcttga tgattggtgc ctactctgac
    31681 ttcattcaga tagtctctca ctctagaaac caatttgtct ggtgtacttg atagaaaaac
    31741 ttcctgcccc tgatttccat ggactgtatt gtcaaatgca aagacagatt ttccagcctg
    31801 tacaaacttg gagacaatag catctacttc ttgaaagtga ttgatatcta gtaaaaagtc
    31861 acaggtatcg actaaaaagt ttagctgttc ttcatttgta atcgttcgga gacggacatt
    31921 atagttcgtt tctaagctac ggattttctc ggaaaattga acaggtgctg caatatgaaa
    31981 acagatattt gacaaggatt caaggagata atccaactct tcaatctgat cactaaccgt
    32041 caaaatcaga gcatctccta ctaaatgatt tttcttcatt tgcttatttc tctcaatttc
    32101 tcaatcattg cttgaacttg gtcaacagca tatgcctcct gacctacttc ataggtttta
    32161 gtattttcaa aggataagat aggcttgcca agattagcaa attgatcgag aatttcttct
    32221 gtcttttcgc catgattaat atctaaaagt acttgactgg tttctaccaa ttcattatcg
    32281 acatctacca aatagtgaat tccgttaaat atagtcacgt ttggataaat tgtcatctga
    32341 gccaatcgat cactaactat tactctagct gctatcttaa actgaatatc aggcaaggat
    32401 tgaaccaatg tctcaatttg ttcaatatgg tctgaggcag tatagattag acaagtgaaa
    32461 ggttccttta ttggatagat gtgagatctt tgtaatggat gtaaatgatg gttttgtccc
    32521 aattctgtcc attcaagccc atgatagtac caccaaactt cacgataagt ttgggccgcc
    32581 aaatctttcc acggttttct atgggaaagg tagtgaataa tagcaggata atcctgaccc
    32641 tcaggcaatt gataatcagc aaactgttta tgaatgacaa tatgattata atcaaaatcc
    32701 aattccagcc atttatgttc aaaaagcata ttcaagatgc tctgatctgc ctgatccacc
    32761 ttatcatgcc attcattggt taaatcaatt aatttttggg tcatattctc ttttttccaa
    32821 aaagcattgt ttaccaagag aacaccggca ttaaagattt cttgaccaaa ataagctctg
    32881 cccccaaaat ctctaacagc agccaaagga taatcttgta agtctgtagc aaacaagtca
    32941 tccagatttt tcgttacaac tagatcacag tccaagtaga gggccttgtc ttcttgcacg
    33001 aaatcagcta tgaaatagcg taaaaagact gtgtaactaa tatccgattt ataacatgaa
    33061 atttgctcag aagttacccg acaattaata atttctgagt caaacttctc taatcgctta
    33121 tttaattgtt tgatccattc atttggaaaa tcgctatgaa tcaaataaaa acgaagcgag
    33181 cgattgtggt aacagataga cctgattgtc gttaaaactt ggtctacata ggcataattc
    33241 gccgctagga caatggcttt cttttcactt gattcttgta ccgttaactg atttaaaagt
    33301 ctttttttca tttcaaactc tttatacgtt gctgtgtcag ataaaccact agcttgaccg
    33361 ttggcgagac tgaattccaa catttgacga taaatcgcca aatgcttttc taaagggtaa
    33421 cccatattag ctagtagcgt aatgcgttcg gacatgacat caaccaatgc atgcatccac
    33481 ttttctgtcc aaactcttga taaactacct tttctaatcc gattattctt catcatcacg
    33541 tttacggcgt ttcgcaaaca aacccaagcc agtcacagct gccaaagctc caaggagtac
    33601 agatgacttg gaagcttctg tacctgtatt tggcaattgc tgtcttgact tacctgctga
    33661 agttgacgcg cttgcgcttg ttgatgccga ttcagacgct gaagccgacg cactcgttga
    33721 tgctgaagct gaagcgcttg tcgatgctga ctcagacgca ctcgttgacg ctgaagccga
    33781 cgcactcgtt gacgctgaag ctgacgcact ggttgacgct gaggctgagg cagacgttga
    33841 tgcggattca gacgcacttg tacttgctga agccgaagcg cttgtcgatg ctgaggctga
    33901 agcactggtt gaggccgatt cagaggcact tgtacttgct gaagccgacg cactcgttga
    33961 cgctgaagct gaggcgcttg tgcttgctga agctgaagca ctcgttgacg ctgaagctga
    34021 cgcactcgtt gatgctgaag ctgaggcact tgtcgatgct gaggctgagg cactcgttga
    34081 cgctgaagct gaggcgctgg tacttgctga agctgatgca ctggttgacg ctgaagctga
    34141 agcactcgtt gatgctgaga ctgatgcact ggttgacgct gaagctgatg cactggttga
    34201 ggccgattca gaggcactcg ttgacgctga agctgaggcg ctggtacttg ctgaagctga
    34261 cgcacttgtt gatgctgaag ccgacgcact cgttgacgct gaagctgagg cgcttgtgct
    34321 tgctgaagct gaagcactcg ttgatgctga ggctgatgca cttgtgcttg ctgatgtact
    34381 tgcgctggtt gacgctgaag ctgaggcact cgttgacgct gaagctgagg cactcgttga
    34441 tgctgaagct gaggcgctgg ttgacgctga agctgaggcg cttgtcgatg ctgatgctga
    34501 tgcgcttgtt gatgcagatt cagaggcgct tgtcaatgct gaagctgagg cactcgttga
    34561 tgctgaagct gaggcactcg ttgatgctga agctgaggcg cttgtcgacg ctgaagccga
    34621 cgcactcgtt gatgctgact cagacgcact tgtacttgct gaagccgacg cactcgttga
    34681 tgctgagact gatgcgctgg tacttgctga ggctgacgca ctggttgacg ctgattcaga
    34741 tgctgatgtg cttgctgagg ctgaggcact cgttgatgct gaagctgagg cgcttgtcga
    34801 cgcggaagct gacgcactcg ttgacgctaa agccgacgca cttgttgatg ccgattcaga
    34861 cgctgaggtg cttgctgaag ctgaggcact cgttgatgcc gattcagacg ctgaggtact
    34921 tgctgagact gatgcgcttg tgcttgctga ggctgaagct gaggcacttg ttgatgctga
    34981 ggctgacgca ctcgttgatg cagattcaga cgcactggtt gatgctgaag ttgaggcact
    35041 agtacttgct gaggctgagg cactggtact tgctgaagcc gacgcactcg ttgaggctga
    35101 agctgaggca ctcgttgagg ctgaagctga ggcactggtt gatgctgatt cagacgctga
    35161 ggtacttgtt gacgctgagg ctgaggcaga cgttgatgct gactcagacg cacttgtact
    35221 tgctgaagct gaggcactcg ttgaggctga agctgaggcg cttgtacttg ctgaagccga
    35281 cgcactggtt gacgctgaag ccgacgcact tgtcgatgct gaggccgaag cactggttga
    35341 cgctgaagcc gacgcacttg ttgaggccga ttcagacgct gaggtgcttg ctgaagctga
    35401 ggcacttgtt gaggccgatt cagacgctga ggtacttgct gaagctgagg cgcttgttga
    35461 tgctgaagct gaagcactcg ttgaggctaa ggctgaggca ctcgttgatg ctgaagctga
    35521 tgcgcttgtc gatgctgaag ccgatgcact cgttgatgct gaagctgagg cgcttgtcga
    35581 tgctgaagcc gacgcactag tacttgctga agccgacgcg ctggttgatg ccgattcaga
    35641 agctgaggtg cttgctgaag ctgatgcact ggtacttgct gaagctgacg cactggtact
    35701 tgctgaagct gacgcactgg ttgacgctga ttcagatgct gatgtacttg ctgaggctga
    35761 cgcactcgtt gacgctgatt cagatgctga ggtacttgct gaagccgatg cacttgttga
    35821 tgcggattca gaggcgctcg ttgatgctga cgtacttgcg ctggttgatg ctgaagccga
    35881 cgcactggtg cttgctgaag ctgaagcgct tgtcgatgct gaggctgaag cacttgttga
    35941 tgcggaagcc gaagcactgg tgcttgctga agctgaagcg cttgtcgatg ctgaggctga
    36001 agcactggtt gaggccgatt cagacgctga ggtacttgct gaagctgatg cactggtact
    36061 tgctgaggct gaggcactgg ttgaggccga ttcagacgct gaggtacttg ctgaagctga
    36121 ggcgcttgtt gatgctgaag ctgaagcact cgttgatgct aaggctgagg cactcgttga
    36181 tgctgaagct gaggcgcttg tcgatgctga agccgatgca ctcgttgatg ctgaagctga
    36241 ggcgcttgtc gatgctgaag ccgacgcact agtacttgct gaagccgacg cgctggttga
    36301 tgccgattca gaagctgagg tgcttgctga agctgatgca ctggtacttg cagaggctga
    36361 ggcgcttgtt gatgcggaag ctgacgcgct ggtgcttgct gaagctgagg cactggtgct
    36421 tgctgaagct gaggcactag tacttgctga agctgaggca ctggttgatg ctgaggttga
    36481 tgcgctcgtt gaggctgaag ctgacgcgct tgtacttgct gaggctgatg cactcgttga
    36541 tgctgaagct gaagcgcttg tcgatgctga ctcagacgca ctcgttgacg ctgaagctga
    36601 ggcgcttgtg cttgctgaag ccgacgcact tgtcgacgct gagactgacg cacttgtact
    36661 tgcggaagcc gaagcacttg ttgatgctga ctcagacgca cttgtacttg cggaagccga
    36721 agcgcttgtc gatgctgact cagacgcact tgtacttgcg gaagccgaag cgcttgtcga
    36781 tgctgaggct gatgcactgg tgcttgctga agctgaagcg cttgtcgatg ctgaggctga
    36841 agcacttgtt gatgcggaag ccgacgcact ggtgcttgct gaagccgacg cactggtgct
    36901 tgctgagact gatgcactgg ttgacgctga agccgatgca ctcgttgatg ctgaagctga
    36961 tgcactggtt gacgctgaag ccgacgcact ggtgcttgct gaagccgatg cacttgttga
    37021 tgcggattca gaggcactgg ttgacgctga gactgaagca ctggttgagg ctgaggctga
    37081 ggcgctagta cttgctgagg ctgaggcact ggttgatgcc gattcagatg cactggttga
    37141 ggccgattca gacgctgagg tacttgctga agctgacgca ctggtacttg ctgaggctga
    37201 tgcactcgtt gatgcggaag ctgacgcact ggtacttgct gaggctgacg cactggttga
    37261 tgctgactca gacgcactcg ttgacgctga agctgacgca ctcgttgacg ctgaagccga
    37321 agcactcgtt gatgctgagg ctgacgcact ggttgatgca gattcagaag ctgatgtgct
    37381 tgctgaggct gacgcactcg ttgatgctga ctcagacgca ctcgttgacg ctgaagccga
    37441 agcacttgtt gatgcggaag ctgacgcact ggtacttgct gactctgatg cactggttga
    37501 cgctgaagcc gaagcactcg ttgatgctga agctgatgca ctagtacttg ctgaggctga
    37561 cgcactcgtt gatgcagatt cagacgcact ggttgatgca gattcagacg ctgaggtact
    37621 tgctgaggct gacgcactcg ttgaggctga agctgaagca ctggtgcttg ctgagactga
    37681 tgcgctggta cttgctgaag ctgaggcact ggttgatgcg gaagctgagg cacttgtcga
    37741 tgctgaagcc gaagcactcg ttgacgctga agccgacgca ctggtgcttg ctgaagctga
    37801 tgcactcgtt gatgcagatt cagacgctga tgtgcttgct gaggctgaag cactggttga
    37861 cgctgaagcc gacgcactgg ttgaagccga cgcactggtg cttgctgaag ctgaggcgct
    37921 tgtgcttgct gaggctgagg cactggttga tgctgaggct gaggcgcttt gactagcact
    37981 tactgagagt gacttagact gtgaaagtga ctgactggtc gtgctagatt caactacctt
    38041 tcctctacca ttgaagtagt tagttctaat gttatctgtt ttagcagcgt aaggggtaaa
    38101 tgtaaaggat gtatccgttc cagtaattgg tacagtccaa gatgatgtta atccatattc
    38161 tctcttagcc tgccaaccat tcatttgggc accatttccc caagtatacc tactaccccg
    38221 cttcgtcatt gtagatgtat tatagtattg aacctgtcca ccatttttgt tagtaatgga
    38281 gttctttaca tctgaagggg taccaagacc actgccaagg gttaacattg tttgtattga
    38341 agttcctgta ttatagatag aatatccatt actcatcttt tttgatatat taccaagagt
    38401 ctttgtttta ggatccacat acgtaaccgt ataggtaaag gtcagtttta agccatcatt
    38461 tgtcactgtc aatcgatagt aaattggaac attatatacc gaatctacac ctttacctgt
    38521 ataaactttt gtctcacttt tagcaatgtt tagacttgca ttaattgcag gggcgccatt
    38581 tacaatcgta ttgccagaaa aaacagcagc ttttatagaa gccagcaatt gctcgatgga
    38641 atccactgaa cgcttgcgtc gcttagcata agattggaga ttgacatttg taactgatgc
    38701 tacataatca ctagctggtt tcttacgatc ttcttcgacc ttcttagcag ttgcttctgt
    38761 agcggcagct gtttgtgatc ctaccacagt agatgatgca gagatacttg tcgaagccga
    38821 tgtacttgct gattctgatg cacttgtact cgctgaggtc gaagcgctgg ttgaggctga
    38881 ctcagatgca gatgtacttg ccgactctga agctgacaaa ctagttgaac tcgctgaatt
    38941 acttgtagat atcgtaccta gaactactgt accatttgtt gccaaagcat ccgttttctc
    39001 tacagttttt tcaagtactg ccgactcatt tgtaaatacc ttcgtttgag ttgcaacggt
    39061 tccacttatg acagttcccg cagcaactat ccccttgagg atatcaagcc cagtaattga
    39121 atgacttact ttatcttcta ccgtttcggt catgacctga gtagtatcaa caccaccacg
    39181 catgacctta aacaagccaa aaagagaagt cgaggcccgt agccaatgct tccctgattt
    39241 gattaatttg aaccgagtta ctcggtctgt ttctctatat tcacccttct gacgtctaaa
    39301 aaatattttg aaatttcctt tcgagtatta ttttcatgta tggatataaa
//
LOCUS       DAVKLW010000013        39092 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKLW010000013 DAVKLW010000000
VERSION     DAVKLW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.013"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            148..282
                     /gene="rpmH"
                     /locus_tag="VW324_000664"
     CDS             148..282
                     /gene="rpmH"
                     /locus_tag="VW324_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9726340.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            566..1459
                     /locus_tag="VW324_000665"
     CDS             566..1459
                     /locus_tag="VW324_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9726341.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVGGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            1764..2537
                     /locus_tag="VW324_000666"
     CDS             1764..2537
                     /locus_tag="VW324_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9726342.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            2537..3097
                     /gene="rnmV"
                     /locus_tag="VW324_000667"
     CDS             2537..3097
                     /gene="rnmV"
                     /locus_tag="VW324_000667"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9726343.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            3169..3990
                     /locus_tag="VW324_000668"
     CDS             3169..3990
                     /locus_tag="VW324_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9726344.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLDTS
                     RTRRPEYAELILEEHMSQIIEKEVYSTNDMLLIRLFFYQMLIRKDLAKFINQIEKLML
                     FLLEQKKVTKLENFFIIRDTLISGMCCLEKVGVTDCFNDYLSCLQEIMDKTQDYQKKP
                     LVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGDGFLVKKLTEEWQEDVKKYL"
     gene            4310..4606
                     /locus_tag="VW324_000669"
     CDS             4310..4606
                     /locus_tag="VW324_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9726345.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            4683..6713
                     /locus_tag="VW324_000670"
     CDS             4683..6713
                     /locus_tag="VW324_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9726346.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            6715..7356
                     /locus_tag="VW324_000671"
     CDS             6715..7356
                     /locus_tag="VW324_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726347.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            7349..7642
                     /locus_tag="VW324_000672"
     CDS             7349..7642
                     /locus_tag="VW324_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726348.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            7893..8765
                     /gene="rsmA"
                     /locus_tag="VW324_000673"
     CDS             7893..8765
                     /gene="rsmA"
                     /locus_tag="VW324_000673"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9726349.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            8767..9645
                     /gene="rsgA"
                     /locus_tag="VW324_000674"
     CDS             8767..9645
                     /gene="rsgA"
                     /locus_tag="VW324_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9726350.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            9656..10312
                     /locus_tag="VW324_000675"
     CDS             9656..10312
                     /locus_tag="VW324_000675"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9726351.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            10275..10937
                     /locus_tag="VW324_000676"
     CDS             10275..10937
                     /locus_tag="VW324_000676"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9726352.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSGYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            10939..12195
                     /locus_tag="VW324_000677"
     CDS             10939..12195
                     /locus_tag="VW324_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9726353.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            12185..13126
                     /locus_tag="VW324_000678"
     CDS             12185..13126
                     /locus_tag="VW324_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9726354.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            13393..14220
                     /gene="purR"
                     /locus_tag="VW324_000679"
     CDS             13393..14220
                     /gene="purR"
                     /locus_tag="VW324_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9726355.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            14287..15537
                     /locus_tag="VW324_000680"
     CDS             14287..15537
                     /locus_tag="VW324_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9726356.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            15663..16457
                     /gene="pflA"
                     /locus_tag="VW324_000681"
     CDS             15663..16457
                     /gene="pflA"
                     /locus_tag="VW324_000681"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9726357.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(16532..17458)
                     /gene="yidC"
                     /locus_tag="VW324_000682"
     CDS             complement(16532..17458)
                     /gene="yidC"
                     /locus_tag="VW324_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9726358.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(17536..17814)
                     /locus_tag="VW324_000683"
     CDS             complement(17536..17814)
                     /locus_tag="VW324_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9726359.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17848..18588
                     /locus_tag="VW324_000684"
     CDS             17848..18588
                     /locus_tag="VW324_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9726360.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18644..19327
                     /locus_tag="VW324_000685"
     CDS             18644..19327
                     /locus_tag="VW324_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9726361.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            19439..20431
                     /locus_tag="VW324_000686"
     CDS             19439..20431
                     /locus_tag="VW324_000686"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9726362.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            20496..21035
                     /gene="rsmD"
                     /locus_tag="VW324_000687"
     CDS             20496..21035
                     /gene="rsmD"
                     /locus_tag="VW324_000687"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9726363.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            21025..21513
                     /gene="coaD"
                     /locus_tag="VW324_000688"
     CDS             21025..21513
                     /gene="coaD"
                     /locus_tag="VW324_000688"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9726364.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            21497..22534
                     /locus_tag="VW324_000689"
     CDS             21497..22534
                     /locus_tag="VW324_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9726365.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            22592..22822
                     /locus_tag="VW324_000690"
     CDS             22592..22822
                     /locus_tag="VW324_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9726366.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            22846..24129
                     /gene="murA"
                     /locus_tag="VW324_000691"
     CDS             22846..24129
                     /gene="murA"
                     /locus_tag="VW324_000691"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9726367.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            24122..24310
                     /locus_tag="VW324_000692"
     CDS             24122..24310
                     /locus_tag="VW324_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9726368.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            24349..25173
                     /gene="endA"
                     /locus_tag="VW324_000693"
     CDS             24349..25173
                     /gene="endA"
                     /locus_tag="VW324_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9726369.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            25464..26795
                     /locus_tag="VW324_000694"
     CDS             25464..26795
                     /locus_tag="VW324_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9726370.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(26993..27063)
                     /locus_tag="VW324_000695"
     tRNA            complement(26993..27063)
                     /locus_tag="VW324_000695"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27029..27031),aa:Cys,seq:gca)
     gene            27479..31090
                     /gene="rpoB"
                     /locus_tag="VW324_000696"
     CDS             27479..31090
                     /gene="rpoB"
                     /locus_tag="VW324_000696"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9726371.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            31123..34800
                     /gene="rpoC"
                     /locus_tag="VW324_000697"
     CDS             31123..34800
                     /gene="rpoC"
                     /locus_tag="VW324_000697"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9726372.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            34911..35324
                     /gene="ndk"
                     /locus_tag="VW324_000698"
     CDS             34911..35324
                     /gene="ndk"
                     /locus_tag="VW324_000698"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9726373.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIGQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(36253..36885)
                     /locus_tag="VW324_000699"
     CDS             complement(36253..36885)
                     /locus_tag="VW324_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726374.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(36887..38902)
                     /locus_tag="VW324_000700"
     CDS             complement(36887..38902)
                     /locus_tag="VW324_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9726375.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
BASE COUNT        11519 a         7551 c         8958 g        11064 t
ORIGIN      
        1 tagggcttgc attcgaaaaa gctggaaaca tttgcctaga gctattgaca aaagtgaaag
       61 aatcagatag aatagtaaag tatgtttagt aactgatcac tttatagccg gctaaacgaa
      121 tacaaaatct atgaggaggt attcatcgtg aaacgtactt atcaaccaag taaacttcgt
      181 cgtgcgcgta aacacggatt ccgtaaccgt atgtcaacta aaaatggtcg tcgcgtattg
      241 gcagctcgtc gtcgtaaagg acgcaaagtt ttggctgcat aatccaaacg aattctatca
      301 aaaatcagta ggaactcgag tctactgatt tttatttttg taaaaaagtt cagtagatgc
      361 aaatggattc ggaagcgatg ttatagtaga ttgaaactag aatagtacac ctctgtttct
      421 aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgtta ttattttatt
      481 ttactataaa gttgaagtag gtggagatgg tacagcaaca atcgtcttta aagatggttc
      541 agctattaca attccaggaa atcaattggt agcacaagat ccaaaagcac aagatagcac
      601 taaaccgact gctgaaaaat caactgttaa agcacctgct caaagagtag atgtaaaaga
      661 tataactcat ttaacagatg aagaaaaagt taaggttgct attttacaag caaatggttc
      721 agcattagac ggagcgacaa tcaatgtagg tggagatggt acagcaacaa tcacattccc
      781 agatggttca gtagtgacga ttctaggaaa agatacagtt caacaatctg cgaaaggtga
      841 atctgtaact caagaagcta cactagagta taagctagaa actataccag gtggagataa
      901 gggaggcaat actggaagct cagatgctaa tgcgaatgaa ggcggtggta gccaggcggg
      961 tggatcagct cacacaggtt cacaaaactc agctcaatca caagcttcta agcaattagc
     1021 tactgaaaaa gaatcagcta aaaatgccat tgaaaaagca gccaaggata agcaggatga
     1081 aatcaaaggc gcaccgcttt ctgataaaga aaaagcagaa cttttagcaa gagtggaagc
     1141 agaaaaacaa gcagctctca aagagattga aaatgcgaaa actatggaag atgtgaagga
     1201 agcagaaacg attggagtgc aagccattgc catggttaca gttcctaaga gaccagtggc
     1261 tcctaatgct gctcctaaga caacaagtgc accgcaagca actgcaggaa caatgcaaga
     1321 tgttacctac cagtcacctg ctggcaaaca attacctaac acaggttcag catcaagtgc
     1381 agcacttgct agtcttggtc tagtggtggc aacaagtggt tttgctttgc taggaagaaa
     1441 gactagacgt agaaaataga acagctagaa aattctattc tctacttaaa gttagattat
     1501 aagggggatt ttgagaagtc atcaatccta gtgatgggtg agaaaagtga gaacccaaga
     1561 taatcacata ctttagctga ataggaatat tctatcaatg tagccaatct cttctgtctc
     1621 taactgtgga ataggagatg ggcaatatcg gatagaaaag atagcagaat agctctctat
     1681 tgaagagagg aggggaaacc gaaaaattag gtgcccctcc tcttttttgg tataatagaa
     1741 gatagaaaac gaggttagaa gagatgattt ttgatacaca tacacacttg aatgtagaag
     1801 aatttgcagg tcgtgaggca gaagaaattg ccttggctgc tgagatgggt gtgatacaga
     1861 tgaatattgt tggttttgat aaaccgacga ttgagcgtgc cttggagttg gtagatgagt
     1921 atgagcagct ctatgcgact attggttggc atcctacaga agctggtact tatacagagg
     1981 aagttgaggc ttacttgttg gataagttaa aacattccaa ggttgtggct ttaggtgaaa
     2041 ttggcttaga ctaccattgg atgacagcgc ccaaagaggt gcaggagcag gtttttcgcc
     2101 gtcagattca gctatctaag gacttggatt tgccttttgt tgtccatacc cgtgatgcgc
     2161 tggaagatac ctatgagatt atcaagagtg agggcgttgg tcctcgtggt ggtatcatgc
     2221 attcattttc agggacgctt gagtgggcag agaagtttgt ggatcttggt atgaccattt
     2281 ccttctcagg agtggtgact ttcaagaagg caactgacct ccaagaagca gctaaagagt
     2341 tacctttgga caagatgttg gtggaaacag atgcgcctta cttagcacct gtacccaagc
     2401 gtggtcgtga aaataaaaca gcctatactc gctatgtggt cgactttatc gctgacttgc
     2461 gtggtatgac gacagaagag ctggcggtag caacgactgc aaatgcagaa cgaatttttg
     2521 gattggacag caagtaatga aagagaaaat ttctcaagtt atcgtggttg aaggtcgcta
     2581 tgatacggtc aatctcaagc gttatttcga tgtggagacc tatgagacac gaggttctgc
     2641 catcaatgat caggatatag agcgaattca gcgtctacac caacgtcatg gagtcattgt
     2701 ctttacagac ccagatttta atggggagcg gattcggcgc atgattatga cggccattcc
     2761 aacagttcag catgcctttc tcaagcgaga tgaagctgtt cccaagtcca agaccaaggg
     2821 gcgttctctg ggaattgagc atgccagcta tgaagacctg aaaacggctc tagctcaggt
     2881 gacagaacaa tttgaacatg agagtcagtt tgacattagt cgtagcgact tgattcgcct
     2941 tggttttcta gcaggggcag acagccgtaa gcgtagagaa tatctcggag agactctccg
     3001 aatcggctat tctaatggca agcaactcct caaacgccta gagttgtttg gggttacttt
     3061 ggcagaagtg gaagaagcta tgaaatctta tgagtaggaa agatgtagcc gttacaattt
     3121 tttaagtttc acagtatttt tcgaagcagg tagaagagga ggcgtctgat gttaattggt
     3181 caaaaaatta aagagattcg gatagaaaaa ggaattagtc gtccagattt ttgtggagat
     3241 gagcaagaac tgacagttcg tcaactgtcg cgaattgaaa gtggagcttc gcaaccgagt
     3301 ttgcccaagt tagcctatat tgctcgccgg ctaggagttc cagtttatag ccttatgccg
     3361 gatttttcag ctcttccttc tgcttattta gaattgaaat accagatttt acgtgaacca
     3421 atctatggta aagaagagga gtatgataag aaagaagcgt gtttggatac ttctagaact
     3481 agaaggcctg aatatgcaga gttaatactt gaggaacata tgtctcagat catagaaaaa
     3541 gaagtttatt caacaaatga tatgttgttg attcgtttgt ttttttatca aatgctcatt
     3601 agaaaagatc ttgccaaatt tataaatcaa atcgaaaagc taatgctctt tcttttggaa
     3661 cagaaaaagg taactaaatt agaaaacttc tttataatta gagatactct tatttcagga
     3721 atgtgttgtc ttgaaaaggt aggagtaact gattgtttta atgattatct atcgtgttta
     3781 caagaaatta tggataaaac tcaagattat caaaagaaac ctcttgtatt tatgtttttg
     3841 tggaagcaag cattaagagt agaaagagat tttagtttag ctgaatcatt ttatcagtct
     3901 tctaaaacat ttgcgaagct aattggagat ggatttctag taaagaaatt gacagaggaa
     3961 tggcaagagg atgtcaaaaa atatttataa acatagtgaa tcagtgacaa aaatgtcctt
     4021 gtcctcgtat caaaacagtt ctaaagttcg tctttagaac tgttttttta gatataagct
     4081 aaaaataata tgaaatagtt agattttaag gacattgatg tccttgatag tgtgataaag
     4141 atggagtata atgactataa aagttcattt cagggtgcta ttttgacaat ttagagaaaa
     4201 acaaataatt ttagaatatc atttggtaat ataatagtat caacgttgat aatggaaaga
     4261 ggagcaagta actcaacaat cttgtaatca aaaatagagg agtatcaata tgaaacaaac
     4321 agttaaaaag ttagctcttg tagcgagcat tgcagcaaca ttaggtggag gtgtatcagt
     4381 tgcctctgca gccgttcaat acccagaagg tggtgtttgg acttatggtt caggtaacgg
     4441 tggtgcttac tcaaactact atcacccttc aaaataccat agttcaacag ttgttagcag
     4501 aaaaactggt tcatctgaca agggatatgc tggtgctgga gggacttctc gtgcatggat
     4561 tcgtacttct tggggagaga aagttgcatt ctactataat gtttagaatg gtatagccct
     4621 attgtttagc tttacttggt caccttctca ctaagtgacc aagtaacttt ttggaggaaa
     4681 tgatgaaacg tttatttatt ttgatttcaa tggtattggt atcgctttat atggtgataa
     4741 cttccgttga ccatcgagaa gagattttat ttggtaacta tccttctgtt gatgtgacag
     4801 gaatgatgat aaatcaacca gtagctagtc gcgaagaggt gacagaggct ttgagtcact
     4861 tggcggtaga gcataatagt ctcatcgccc gtcgaatcgt tgagccaaat gaagctggag
     4921 aaacactctt tacctatgcc acttatggtg agggagagct tccagaaggt ctgaccattt
     4981 cctccaagga gagtgcagaa acgagtgatt tattagggtc ttacttgatt gtgtcaggaa
     5041 gtttggatgg agtgagctta cagaccacct tgaaagagct tggttatcaa ggctttgttt
     5101 cgaatggaga agatccattt tcgatagtct tactattggc ggccacccct atggtgctac
     5161 tgagtttagc tatttttctg ctgaccttta tgagtctgac cctgatttat cggatcaaat
     5221 cccttcgtca ggcagggatt cgcttaatag ctggtgagag cttgtttgga gttgctctca
     5281 gaccagtgtt agaagatgtg agacagctta tctgctcagt gctggtatcc agtcttttgg
     5341 gattggggat tctctggtat caaggtgcct tgtttatggc aacggtacaa ctggtcatca
     5401 ttgctcttct actttatgga ttgaccttgg cagggatttc taccttacta agtgtcgtct
     5461 atctacttgg tttacaggaa aatagtctgg tggatctatt gaaagggaaa ctccctctca
     5521 aacgtatgat gacattgatg atggtggggc aactcttagc tgtattggtg gtcggatcga
     5581 gtgcgacagc tctcctaccc cactaccgtg aaatgcagga aatggagaga gctagcaata
     5641 aatggagcca gtcctcagac cgttaccgtc tatcctttgg ttggtctagt gcatttgccg
     5701 atgaagaagg aacgcgtaag gataatcgtg agtggcagac atttactgaa gaacggttag
     5761 ccaatacaga ctctttttat attatgagca atgttgacaa tttctcagat ggagcagaag
     5821 tggacctaga tggcaatcgt ctcagtgact acacaccgtt agggaatgtt atctatgtct
     5881 cgccgcgcta tctgatagaa gaaaagatta ccgtttcttc agagtttatg gacaagatgc
     5941 aaaacttgtc tgagggagag tttgggctga tcttgcctga gagcttgcga gagcagtctg
     6001 cctactacca aggattgttt acagattacc tgcaaaactt ttcatctgaa agtgtagaag
     6061 tgacgagtca gaaacactac ctcccacagg taaggctagc ttttacagaa acaggacagg
     6121 aacgtttcct ctataatgat gggtacaaga caacacgcca gtacctaaaa gatccgatta
     6181 ttgtagttct aacgccgcaa gcgactggaa caagacctgt tgcagggatg ttgtggggaa
     6241 ctacggctaa tagtgccttg aaactagatc gatatggaga cagcatcaca gctctaaaag
     6301 agcaaggtct gtatcacaag gtttcttact tggtaaaaag ccagctattt tttgccaagg
     6361 tactaaatga caaacgggtg gagttttact ctctccttat tgggacgatt ttgaccctgt
     6421 ctacggctat cttgttattt gattccatga atcttctcta ttttgagcag ttcagacggg
     6481 aacttatgat taaacgtctt gctggtatga caatctatga gcttcatggc aagtatttac
     6541 tggcgcaagg aggagttctc ttgcttggcc tagtcctatc tagtattttg acaagagatg
     6601 gtttgattag cgctctagtt gtagctttgt ttacgcttaa cgccctcttg attttagtaa
     6661 ggcaggataa aaaagaagaa gctggtagca tggcagtatt gaaaggaaaa taagatgatt
     6721 gatattcaag gattggaaaa gaaatttaat gaccgcgcga ttttctctgg tttgaatctc
     6781 aagctggaga agggcaaggt ttatgcctta atcggaaaga gtggaagcgg aaagacgacg
     6841 ctgctgaata tcttgggaaa gctagaaaag atagatggtg gaagggttct ctatcagggg
     6901 aaagatttaa aaaccattcc tactcgtgag tattttcgag accagatggg ctatctcttt
     6961 caaaatttcg gcctcttaga aaaccaatca atcaaagaaa atttggattt gggttttgtt
     7021 ggtcagaaaa tctcaaaagt agaacgtttg gaaaggcaag tgggggcttt agaaaaagtt
     7081 aatctagggt atttggattt agaacaaaaa atttatactt tatctggggg agaggcccaa
     7141 cgagttgccc ttgctaagac tattttgaaa aatccaccct tgattttggc agatgaacca
     7201 acagcagctc ttgatcctga aaattcagag gaggttatga atctcttggt ggatttgaaa
     7261 gatgaaaatc gaattatcat cattgcgacc cataatcccc tagtctggaa taaggctgat
     7321 gaaatcattg atatgaggaa acttgctcat gtgtgaaaaa atccgtattc gcagggtatc
     7381 tgattatcct agtgccagag gtggtttaga agatatcctc atcatggaaa atatgaccaa
     7441 tcatctcctt ttggttcaaa tccgagtgca tggctatttg cttgattttg ctagtattga
     7501 agggcaaagg caaaagcatt atcgtttgaa aaatttacct cagacggttg aactgacagt
     7561 ggatgatgtg gaggaggatg tggatttgac cctacccgaa aatcgaagtt atcaagaagc
     7621 tgattttttt gatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgt
     7681 acttgagtac ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtatgaacg
     7741 tatgtttcga gagaagcact aagtccctct ttaaagattt ccaagactat ctttcttcat
     7801 gaggaaagat agttttttgg tatgattttc attcccaaaa tacaagggga atgtgttaca
     7861 atagtagtaa cagataatag aaaagagaat agatgagaat tgcagattat agcgtgacca
     7921 aggcagtgtt ggagcgtcac ggttttacct ttaaaaagtc ctttgggcaa aatttcttga
     7981 cggataccaa tatccttcaa aaaattgtgg atacggctga aattgatgat caggtcaatg
     8041 tcatcgaaat cgggccaggt attggtgcct tgacagaatt tttggctgag cgtgcagccc
     8101 aagtcatggc ttttgagatt gaccaccgtt tgatgccaat tttggcagat actctgcgtg
     8161 attttgataa tgtgaccgta gttaacgaag atattctcaa ggttgatttg gcgcaacata
     8221 tccaaaattt taaaaatcct aacctgccaa tcaaggtagt ggctaatttg ccttactaca
     8281 tcacgacgcc tattctcatg cacttgattg agagtggcat tcctttttgt gagtttgtgg
     8341 tcatgatgca gaaagaagta gcggaccgca tttcagccca gcctaacacc aaggcttacg
     8401 gtagcttgtc tatcgccgtg cagtattaca tgacagccaa ggttgccttt atcgtgcctc
     8461 gtacggtctt tgtgccagcg ccaaatgtgg attcagccat cttgaaaatg gtgcgtcgtc
     8521 cagagccagc cgtagcagta gaagatgaga actttttctt taaggtttcc aaggctagtt
     8581 ttacccatcg ccgcaagacc ttgtggaata acttgacagg ttactttggt aagactgaag
     8641 aggtcaagga caagctgacc aaggctttgg accaggcagg cttgtcacca agtgtgcgtg
     8701 gggaagctct cagcttggca gaatttgccg gtctagcaga cgcacttaaa gggcaaggac
     8761 tctaagatgc agggacaaat cattaaagcc ttggcaggtt tctactatgt ggagagtgat
     8821 ggccaggttt atcaaacacg cgcgcgtggg aatttccgta aaaaaggcca taccccttat
     8881 gttggggact gggtagattt ctctgccgag gaaaattcag aaggctatat cctcaaaatt
     8941 cacgaacgga aaaacagtct ggttcgtccg cctattgtca atatcgatca agctgtagta
     9001 atcatgtccg tcaaggaacc tgattttaac agcaatttgc tggatcgttt cttggttctt
     9061 ttggagcaca agggcatcca tcccattgtc tatatttcca aaatggattt gttggaagat
     9121 aggggagaac tggattttta ccagcagacc tatggtgaca tcggctatga ctttgtgacc
     9181 agtaaagagg aactcctgtc tttgttaaca ggcaaggtta cggtctttat ggggcagaca
     9241 ggtgttggga agtcaactct tctcaataaa atcgcaccag acctcaatct tgaaacggga
     9301 gaaatttcag acagtctagg tcgcggtcgc cataccactc gagctgttag tttttacaat
     9361 ctcaacgggg gtaaaatcgc agatacacca ggcttttcat ctctggacta tgaagtatca
     9421 agggctgaag acctcaatca ggcctttcca gagattgcta ctgttagccg agattgtaag
     9481 ttccgtactt gtacccatac ccatgagccg tcttgtgccg tcaaaccagc tgttgaagag
     9541 ggtgttattg caaccttccg ttttgacaat tacctgcaat tccttagtga aattgaaaat
     9601 cgtagagaaa cctataaaaa agtcagcaaa aaaattccaa aataaggaga aacctatgtc
     9661 tcaatacaag attgctccgt caattctggc agcagattat gccaactttg aacgtgaaat
     9721 caaacgtcta gaagcaactg gggcagaata tgcccatatc gatatcatgg acagtcattt
     9781 tgtaccgcaa atcagttttg gtgcaggtgt ggtcgagagc cttcgtcctc atagtaagat
     9841 ggttttcgat tgccacttga tggtgtcaaa ccctgagcat catctggaag attttgcgcg
     9901 tgcaggtgca gacatcatca gtatccatgt agaagcaacg cctcatattc atggcgccct
     9961 ccaaaaaatt cgttcactcg gagttaagcc ttcagtcgtt atcaatcctg gcacatcagt
    10021 tgaagccatc aagcacgtcc ttcatctagt tgaccaagtt ttagtcatga cggttaatcc
    10081 aggttttggt gggcaagcct ttctgccaga aaccatggat aaggttcgtg agttggttgc
    10141 tcttcgtgag gaaaaaggtt tgaactttga aatcgaagtg gatggtggga ttgatgacca
    10201 aactattgcc caagccaaag aagctggtgc gaccgttttt gtagcaggtt cctatgtctt
    10261 taagggagat gtcaatgagc gagtacaaac tctcagaaaa caactggact agggttgccg
    10321 tttttgcagg cggagaccgc ggtcattatc ggacagattt tgatgctttt gttggggtgg
    10381 atcgaggctc gctctgggtc ttggaagaag acttgcttct tgctctagca gttggggatt
    10441 ttgattctgt gaccgaagaa gagcgacagg tgattcaaaa acgtgcccag cattttgtcc
    10501 aagcccgacc agaaaaagat gataccgatt tggaattggc tctcttaacc atctttgaga
    10561 aaaatcctca ggctcaggtt actattttcg gtgccttggg tggccgtatt gaccatatgt
    10621 tggccaatgt ctttctgcct agcaatccta agttggcacc ctatatgcat caaatagaaa
    10681 ttgaggatgg gcaaaacttg attacttatt gtccagaagg gaccagtcag ctagaacctc
    10741 gttcaggcta cgactatcta gcctttatgc cagttcggga tagccagctg actattcttg
    10801 gagccaagta tgagttgaca gaggaaaatt ttttctttaa aaaagtgtac gcttctaacg
    10861 aatatataga tagggaagtg tcggtaactt gcccagatgg ttatgtggtc gtactgcata
    10921 gcaaggacag gaggtaggat ggaaagttta cttattctat tattaattgc caacctagct
    10981 ggtctctttc tgatttggca aaggcaggat aggcaggaga aacacttaag taagagcttg
    11041 gaggatcagg cagatcattt gtcagaccag ttggattacc gctttgacca agccagacaa
    11101 gccagccagt tagaccaaaa agatttggaa gtggttgtca gcgaccgttt gcaagaagtg
    11161 cggattgaat tgcaccaagg tctgacccaa gtccgtcaag aaatgacaga taatctcctc
    11221 caaactagag acaagacaga ccaacgtctc caagccttgc aggaatcaaa tgagcaacgt
    11281 ttggaacaaa tgcgccagac ggtcgaggaa aaactagaaa agaccttgca gacacgctta
    11341 caggcttcct ttgagacagt ttctaaacaa ctggagtctg tcaatcgtgg ccttggagaa
    11401 atgcagacag ttgcccgtga tgtcggagct cttaacaagg ttctctctgg aaccaagacg
    11461 cgagggattc tgggagaatt gcaactgggg caaattattg aagacatcat gacacctgcc
    11521 cagtacgaac gagaatacgc aacggttgaa aactctagtg aacgagtgga gtatgccatc
    11581 aagttacccg gacaaggcga ccaagaatac gtctatctgc caattgactc taagtttcca
    11641 ctggcagatt attaccgctt ggaagaagcc tatgagacag gtgacaagga tgagattgaa
    11701 cgctgtcgta agtcactcct agcaagcgtc aagcgctttg ccaaggatat caagagcaag
    11761 tacatagcac cgcctcggac gaccaatttt ggagttttgt ttgttccgac agaaggtctt
    11821 tattcagaga ttgtgcgcaa tccggtcttc tttgatgatt tgagacggga agaacagatt
    11881 atcgttgcag gaccaagtac cctatcagcc cttcttaact ccctatcagt tggtttcaag
    11941 acccttaata tccaaaagag tgccgaccat atcagcaaga ctcttgccag tgtcaagacc
    12001 gagtttggca agtttggtgg tattctggtc aaggcacaaa aacatctcca acatgcctct
    12061 ggcaatattg atgaattatt aaaccgtcgt accatagcta tcgagcggac gctccgtcac
    12121 attgagttgt cagaaggcga gcctgcgctt gatctactcc attttcaaga aaatgaggaa
    12181 gaatatgaag attagtcaca tgaaaaaaga tgagctattt gaaggctttt acctaatcaa
    12241 atcagctgac ctgaggcaaa ctcgagctgg gaaaaactac ctagccttta ccttccaaga
    12301 tgatagtggc gagattgatg ggaagctctg ggatgcccaa cctcataaca ttgaggcctt
    12361 taccgcaggt aaggttgtcc acatgaaagg acgccgagaa gtttataaca atacccctca
    12421 agtcaatcaa attactctcc gcctgcctca agctggtgaa cccaatgacc cagccgattt
    12481 caaggtcaag tcaccagttg atgtcaagga aattcgtgac tacatgtcgc aaatgatttt
    12541 caaaattgaa aatcctgtct ggcaacggat tgtccgaaat ctctacacca agtatgataa
    12601 ggaattctac tcctatccag ctgccaagac caaccaccat gcctttgaaa cgggcttggc
    12661 ctatcatacg gcgaccatgg tgcgtttggc agacgctatt agcgaagttt atcctcagct
    12721 caataagagc ctgctctatg cggggattat gttgcatgac ttagctaagg tcatcgagtt
    12781 gacggggcca gaccagacag agtacacagt gcgaggtaat cttcttggac atatcgctct
    12841 cattgatagc gaaattacca agacagttat ggaactcggc atcgatgata ccaaggaaga
    12901 agtcgttttg cttcgtcatg tcatcctcag tcaccacggc ttgcttgagt atggaagccc
    12961 agtccgtcca cgcattatgg aagcagagat tatccatatg attgacaatc tggatgcaag
    13021 catgatgatg atgtcaacag ctcttgcttt ggtggataaa ggagagatga ccaataaaat
    13081 cttcgctatg gataatcgtt ccttctataa accagattta gattaataat ttaagaaaaa
    13141 tgagcatttt ttaggataag aatgttcgtt tttttatgtg aatatggtat aataagtaaa
    13201 agacaaaaat gaatactctt cgaaaatctc ttcaaactag ggtagtatcg ccttgtcgta
    13261 tgtatatatg caggtatatt acagggtttg tcagttctat tgacaatctc aaaacagtgt
    13321 tttgaaccac cagcgaccag ctttctagtt tgctttttga ttttttgaat aaaaatggaa
    13381 taggaaatag aaatgaaatt aagaagaagt gatcggatgg ttgtcatttc caactatttg
    13441 attaataatc cttataaact aactagtctc aatacttttg ctgaaaagta tgagtctgct
    13501 aaatcatcca tctcagaaga tatcgtcatt atcaaacgcg cctttgagga aattgaaatc
    13561 ggtcatatcc agacagtgac tggggctggc ggaggtgtca tcttcacacc gtctatttcg
    13621 agtcaggatg ctaaggaaat ggttgaagac ttgcgtacca agttgtcaga aagtgaccgt
    13681 atcttgccag gtggttatat ctatctgtct gatttgctta gcacaccagc catcttgaaa
    13741 aatattggtc gtattattgc caaaagcttt atggaccaaa aaattgacgc ggttatgacc
    13801 gtagcaacta agggtgtgcc acttgcaaat gcagttgcca atgtcctcaa tgtctctttt
    13861 gtcattgtgc gccgtgacct gaaaattacc gaaggttcaa ctgttagcgt caactatgtt
    13921 tcaggttcaa gtggtgaccg tatcgagaaa atgttccttt caaaacgtag tcttaaggca
    13981 ggtagccgtg tcttgattgt ggatgacttc ttgaaaggtg gcggaacggt caacggtatg
    14041 attagtctct tgcgcgagtt cgactcagaa ctggcaggtg tagcggtctt tgcggacaat
    14101 gcccaagaag aacgtgaaaa gcagtttgac tacaagtcac tcttgaaggt aactaatatt
    14161 gatgtcaaga accaagccat cgatgttgag gttggcaata tctttgacga agataaataa
    14221 gagatagaac taaaggttgg aacgattgtc ccagcctttc tttgcaaaca gaatagaagg
    14281 aagcttatga aaacaccatt tatcaataga gaagagttag aagcgattgt tgccgagttc
    14341 ccgactccct ttcacttgta tgatgagaag gggattcgtg agaaggcaag agccgtcaac
    14401 caagcttttt cgtggaacaa gggctttaag gaatattttg cagttaaggc tactccaact
    14461 ccagctattt tgaaaattct ccaagaagaa ggttgtggtg tggactgctc tagttatgta
    14521 gagcttttga tgagccataa actggacttt ctgggttctg agattatgtt ctcttccaac
    14581 aacacgccag acaaggaata cgcctatgca cgtgaattgg gtgcgaccat taacttggat
    14641 gcctttgaag atattgaaca tctggagaga gtagcaggca ttccagaaat catctcttgt
    14701 cgttataatc ctggaggcgt ttttgaactg gggacagaca ttatggacaa tcctggggag
    14761 gctaagtttg gcatgaccaa ggaccagctc tttgaagcct ttgctatctt gaaggaaaaa
    14821 ggagccaaga cttttgggat tcactccttc ctagcgtcca ataccgtgac ccatctctat
    14881 tatccagagt tggctcgtca gctctttgaa ctggctgttg aaatcaagga aaagttgggc
    14941 atttggctag actttatcaa tctttctggc ggtattggtg ttaattatcg tccagaccag
    15001 gagccgaacg atatcgcctt gattggtgag ggagttcgta aggtgtatga agaagttctt
    15061 acgtcagcag gtcttggtca ggtcaagatt ttcaccgaat tgggtcgttt tatgctggca
    15121 cctcacggtg ctctagtcac aagagtgact cataagaagg aaacctaccg tacctatcta
    15181 ggtgtggatg cctcagcagt caacctcatg cgtccagcta tgtacggagc ttaccatcat
    15241 attagcaacg tgacccatcc agatggacca gctgaagcgg tagatgtggt tggttcactc
    15301 tgtgaaaaca atgataaatt tgcagtcaat cgcgaactgc ctcatacaga aatcggtgat
    15361 ttgctggtaa ttcatgatac aggtgcccac ggattttcaa tgggctacca gtataatgcc
    15421 aaattacgtt ctgcggaaat cctctatacc gaagaaggta aagcccgtca aatccgccgt
    15481 gcagagcgcc ctgaggacta ttttgcaacc ttatatggct tcgattttga agaataatct
    15541 gataatagat tgaaaatgaa attgaaaaac agattgcttt ctaaaaaata ggcaaaaatc
    15601 ttgtttttcc ttcaagtcgt gatataataa aactataaaa cgttttcaag gaaggtaacg
    15661 atatgtctga agaaacaatt gattatggac aagtgacagg aatggtgcat tcgacagaaa
    15721 gctttgggtc agtagatggg cctggtattc gctttattgt ctttttgcag ggctgtcaca
    15781 tgcgttgcca gtattgccac aacccagaca cttgggctat ggagtccaat aagtcacgtg
    15841 aacggacggt agatgatgtc ttgacagagg ccttgcgcta ccgtggtttc tggggaaata
    15901 agggtgggat tacagtcagt ggaggagaag ctctcttgca gattgatttc ctgattgctc
    15961 tcttcaccaa ggctaaggaa caaggaatcc actgtacctt ggatacctgt gctcttcctt
    16021 tccgtaataa accacgttac cttgagaagt ttgacaaact catggctgtc actgacttgg
    16081 ttcttttgga tatcaaggaa atcaacgaag aacagcacaa gattgtcact agacaaacca
    16141 ataaaaatat cttggcttgt gcccagtatc tatcagatat tggaaaacct gtctggattc
    16201 gccacgtgct agttccagga ttgacagaca gagatgatga cttgattgaa cttggtaagt
    16261 tcgtcaagac cctcaaaaat gttgataagt ttgaaattct accttatcac accatgggtg
    16321 agttcaagtg gcgtgaactt ggaattccat attccctcga aggagtcaaa ccaccaacag
    16381 cagatcgcgt caagaacgct aaaaaactca tggataccga aagttatcaa gattatatga
    16441 aacgtgtaca tggatagaaa agaagcctga tggaaacatc gggcttttga tttgcaaaaa
    16501 gacttagcaa atcagctaag cctttttctt cttatctcga acgttgtttt ccagcgttgc
    16561 gatttttgtg ttttttcttg cttgtgatag cagttggttg ttcaggggta acgtcttttc
    16621 gtccacttgg tttagagaaa gcacttgctt ttggtgggtt cttggctagt tcttcacgga
    16681 cttttttgcg aagttttgga cgaacgatat agttgacgat aaactgttgg agaatcatca
    16741 tgaaaccacc gacaacccag taaagtgtga cactagctgg tgagaagagg gagaagacga
    16801 cgatcatgag tgggctcatg taaatcattt tcttgatttg ttctctttgc atttcatctt
    16861 ctactccgtg aagtgaaagg agcgattgaa gatagtaaag gacaccagca caggcaacca
    16921 aaatcatact tggagaacct agaggaatgc ctaggtagct tgcttgagca accccttcag
    16981 tatgttgggc agcaaagtag atagcagaga agaaaggcat ttgaaggagg atagggaaac
    17041 atcctacacc gccaaacatg ctgataccgt gctctttttg agcagcaaag agagcttgtt
    17101 gggcttcgag tttttcttct tgagtagtcg cttctttgag acgcgtttgg tgtggctcaa
    17161 ggacgtgctt gagggcgttc atcttttcag agtgaagcgt tgccttccat gattggtaga
    17221 taccaagtgg caagataatc aagcgcacga taatggttac gatgatgata gcgacaccaa
    17281 agcctagacc tttatcagta gcgaagtact tgatagcttc agccataggg gctccgatgg
    17341 tattccaaat aaatcctgtt ggctgacctg tggttttatc gacattgaca caaccagtca
    17401 agacaagcaa catagccgct cccatagctg agagtgcaaa acgtttaata gatttcactg
    17461 tttttattcc ttctttaaaa attatacctt tctattctac tgttttttta caaaatatac
    17521 aatagttctg gagacctaat ttgcgatttt aaagtcagag tagggaggaa agttgcttag
    17581 tttgacatct aagtagctta cttttgaaaa aggtgtcggt ccttttcgga tttcttggat
    17641 aaattttgcc atgatagcag atgagtctgc ttgggctaag atttccactg tgccatcgtc
    17701 gttattccat actcgacctg tgatgccacc aatttcaagt gccaagctgt aaacacccca
    17761 acgaaagccg actccctgta ccctaccttg ggcaatcatt ctaacctttt gcataccaaa
    17821 cccctttgat tgtgttataa tatttctatg actattataa cctcaaaagc caattctgtg
    17881 gtaaaaaatg ccaagaaatt acgccaaaaa aaataccgca agtctgccta cttgattgaa
    17941 ggctggcact tgtttgaaga agctgtccaa gctggagtga cgattgagaa aatctttgcc
    18001 ctagaaaatt atcgagatca gttagctact ttttcgcaaa ctgtctgggt gtcagaggat
    18061 attttgctag atttggcgga ttctcagact ccacagggaa ttgttgccgt ggttcaaaaa
    18121 gaagaagtag gacaagctga tttgagtcag ggcaagttct tgtttttgga agatgtgcaa
    18181 gatcctggta atgtaggaac tatcattcga actgcggatg cagcaggttt tacaggagtg
    18241 attgtttcag ataagtcggc agacatctac agtctcaaaa ccctacgttc catgcaaggt
    18301 agtcatttcc atctgcccat ttaccggatg actagtcaag cgcttcttga cgaaactaaa
    18361 aaggtagcta tcccagtgct agcaacaacc ctatctaaag attctgttga ttacagagaa
    18421 ctgccttcta tagaaaattt tgtactagtt atgggaaatg agggtcaagg aattagtccc
    18481 cttatggctg aaagtgcaga ccagttggtc catattagca tgaaggggca ggccgagagt
    18541 ttgaatgttg cggttgcagc cggtatttta atcttccatt taagctaatt ttaacttttt
    18601 ttgttataat caaggaaaga tgttcacaga aaaggagaaa tggatgaatc acactattat
    18661 acatgaccgt gcaggtctca atcaatttta cgctaaggtt tatgcctttg ttggtctggg
    18721 aatcggacta tctgctttgg tatcaggcct tatgttgacg gtctttcagt ctcagttggt
    18781 ttactttttg atgcaggggc gtctctggtt gaccattgct acttttgcgg agttggcttt
    18841 ggttttcgtt gccagtagca tggcttcaag aaatagtcca gctgctcttc cagtattttt
    18901 actttactcc gttttaaacg gctttaccct tagttttgtg gtggctttct atactccggg
    18961 tacagtttta tctgcctttg tatcgagcgc ccttctcttc tttgtcatgg cggcagttgg
    19021 tatttttacc aagaaagact tgagtgggat tggtcgagca atgatggcag ctctcattgg
    19081 tttactgatt gctatggtag tcaatatttt cttggctagt ggcttctttg attatatgat
    19141 tagcgtagcc atggttttgg ttttttcagg gttgattgct tgggataacc aaaagattcg
    19201 cctcgcctat gaacaatcac aaggtcgtgt agcgacaggc tgggtggtgt caatggctct
    19261 cagcatctat cttgatttta tcaatctttt cctaagtatt ctacgtatct ttggtcgcaa
    19321 tgactaaaag gtctatgaaa tcagtgtttt gagtgacact gatttttttt aaattttctc
    19381 ttttcttttc ttggaaatag gtgtataata cgtttattaa atttttgagg agttgtctat
    19441 gaagaaaagt tttatccatc aacaagaaga aatttccttt gtcaaaaaca cttttaccca
    19501 gtatttgaaa gataagctag aagttgtcga agttcaaggt cctatcttga gtaaggtcgg
    19561 tgacggaatg caggacaacc tgtctggtgt ggaaaatcca gtatcggtca aggttctcca
    19621 aatccctgat gctacttatg aagtggtgca ctcacttgct aaatggaaac gccacacctt
    19681 ggctcgtttt ggctttggtg aaggagaggg tctctttgtc cacatgaaag cccttcgtcc
    19741 agatgaggat tccttggatg caacccactc tgtttatgtt gaccagtggg attgggagaa
    19801 ggttatccca aatggtaagc gtaacatcgt ttatctaaaa gaaacagttg agaagattta
    19861 taaggctatt cgcctgactg agctagctgt tgaagcccgc tatgacatcg agtctatctt
    19921 gccaaaacaa atcaccttta tccatacaga agaattggta gaacgctacc cagacttgac
    19981 acctaaagaa cgtgaaaatg cgatttgtaa agaatttgga gccgtctttt tgattggtat
    20041 cggtggcgag ttgccagatg gtaaaccgca cgatggacgt gcaccagact atgatgactg
    20101 gacaagcgag tctgagaatg gctacaaggg tctaaatggt gatattcttg tctggaatga
    20161 gtctttaggt ggagcctttg agttgtcttc tatgggaatt cgtgtagatg aagaaactct
    20221 tagacgtcag gtggaaatta caggtgatga agaccgcttg gaattggaat ggcacaagtc
    20281 tttgttgaat ggcctattcc cattgacaat cggtggagga attggacaat ctcgtatggc
    20341 catgttccta cttcgcaaga gacacatcgg agaagtgcaa acaagtgttt ggcctcaaga
    20401 agtccgcgat acttacgaaa atattttgta gagaatcgaa ccgcaaggtt cggttttctt
    20461 tctctttttg tctataattt ggtataataa acagtatgaa aatcgtatca ggaatctatg
    20521 ggggacgtcc cctcaagaca ctagaaggca agacaacaag acctacttcg gataaggtta
    20581 ggggagccat ttttaacatg attggtccct actttgaagg gggacgagtc ttggaccttt
    20641 atgcaggtag tggtggttta tctatcgaag cagtatcgcg tggcatgtcc agtgctgttt
    20701 tggtggagcg agaccgtaag gctcagacca tcgtggctga aaatatccag atgaccaagg
    20761 aagttggaaa atttcaactc ctcaagatgg atgcagagag ggcattggaa caggtatctg
    20821 gggaatttga cctcgttttc ttagaccctc cctatgccaa ggaacaaatc gtagcagata
    20881 ttgaaaaaat ggctaagaga gagctttttt ctgaagatgt tatggttgtg tgcgagacgg
    20941 ataaggccgt tgaacttcca gaagagattg cctgtctggg tatctggaag gaaaagattt
    21001 atggaattag taaggtgaca gtctatgtca gataagattg gcttattcac aggctcattt
    21061 gatccgatga caaatgggca tctggatatc attgaacggg cgagcagact ttttgataag
    21121 ctttatgtgg gtattttttt taatccccac aaacaaggat ttctccctct tgaaaatcgt
    21181 aaacgggggc tagaaaaggc tttgggacat ttggaaaatg ttgaagtcgt ggcttctcat
    21241 gatgaattgg tggtcgatgt tgcaaaaaga ctgggggcta ctttcctagt acgggggttg
    21301 aggaatgcgg cggatttgca atatgaagct agttttgatt actacaatca tcagctgtct
    21361 tctgatatag agactattta tttacatagc cggcctgaac atctctatat cagttcatca
    21421 ggtgttcgag agcttttgaa gtttggtcag gatattgcct gccatgttcc cgagagtatt
    21481 ttggaggaaa taagaaatga aaaaaaagat tagatggccc ttatacgtca ttgcggcctt
    21541 gattgtgact ttcttggcat ttgtagtgcc cttgccttat tatatagagg ttccaggtgg
    21601 ttcggaagat attcgccaag tccttaaagt aaatgacaca gaagataagg aagctggtgc
    21661 ctatcaattc gttacggttg gtgttcaaca tgccacttta gctcatatga tttatgcttg
    21721 gttgacacct tttacagata ttcgtagtgc tcaggagact acaggtggtt cttccgatgt
    21781 tgaatttatg cgaatcaatc aattctacat gcaaacatcg caaaatatgg ccaagtatca
    21841 aggactaaaa acagctggta aggatatcga actcaagtac tttggagttt atgttttgaa
    21901 tgtgacggat aattcaacct ttaaagggat tctcaatatc tctgatacag tcacagcagt
    21961 caatgatcag acctttgata gttccaaaga cttgattgat tacgtcagtt ctcaaaaatt
    22021 aggggattcc gtcaaggtca cctatgaaga ggatgggcaa accaagtctg cagaaggaaa
    22081 gattatcacc ttggaaaatg gcaaaaatgg aattggaatc ggcttgattg accgtacaga
    22141 ggtaatcagc aatgtcccaa ttagcttttc aacagctggt attggcggcc caagtgctgg
    22201 tctcatgttt agtctagcta tctatactca aatagctcac ccagatcttc gtaatggtcg
    22261 tattgttgcc ggtacaggta ccattgaccg cgatggtaat gtgggagaca ttggaggtat
    22321 tgataagaag gttgtagctt cggctagggc aggtgctgct attttctttg ctcctgataa
    22381 ccctgttagc gaagaagaac aaaaggcgca tctggacgcg aaaaacaact accaaacagc
    22441 cctagaagcg gctaaaacaa tcaagacgga tatgaaaatc gtgcccgtta aaaccctaca
    22501 agatgcgatt gattacttga aaaacaatcc ctaatttttg agtgaagttt ccaaactagc
    22561 gcacaaacag agaagatggt ataatagtca aatggttcaa ttattattca ctctaagtag
    22621 tcatatgctc tttatttatg tgagttttta ccttttaaag gatcttgtta gatgggagaa
    22681 ggttttaaaa gtgacagatg ataatacaag aaaagttcgt ttattagtag ccttttttag
    22741 cattgtcata ggctacatcc tgagttcttt ctttattagc ctgtatcatt tgtggcaaga
    22801 agcgcttaga ggattattat aaaatcaaga gtaaaggaaa cgagtatgga taaaattgtg
    22861 gttcaaggtg gcgataatcg tctggtagga agcgtgacga tcgagggagc aaaaaatgca
    22921 gtcttaccct tgttggcagc gactattcta gcaagtgaag gaaagaccgt cttgcagaat
    22981 gttccgattt tgtcggatgt ctttattatg aatcaggtag ttggtggttt gaatgccaag
    23041 gttgactttg atgaggaagc tcatcttgtc aaggtggatg ctactggcga catcactgag
    23101 gaagcccctt acaagtatgt cagcaagatg cgcgcctcca tcgttgtatt agggccaatc
    23161 cttgcccgtg tgggtcatgc caaggtatcc atgccaggtg gttgtacgat tggtagccgt
    23221 cctattgatc ttcatttgaa aggtctggaa gctatggggg ttaagattag tcagacagct
    23281 ggttacatcg aagccaaggc agaacgcttg catggcgctc atatctatat ggactttcca
    23341 agtgttggtg caacgcagaa cttgatgatg gcagcgactc tggctgatgg ggtgacagtg
    23401 attgagaatg ctgcgcgtga gcctgagatt gttgacttag ccattctcct taatgaaatg
    23461 ggagccaagg tcaaaggtgc tggtacagag actataacca ttactggtgt tgagaaactt
    23521 catggtacga ctcacaatgt agtccaagac cgtatcgaag caggaacctt tatggtagct
    23581 gctgccatga ctggtggtga tgtcttgatt cgagacgctg tctgggagca caaccgtccc
    23641 ttgattgcca agttacttga aatgggtgtt gaagtaattg aagaagacga aggaattcgt
    23701 gttcgttctc aactagaaaa tctaaaagct gttcatgtga aaaccttgcc ccacccagga
    23761 tttccaacag atatgcaggc tcaatttaca gccttgatga cagttgcaaa aggcgaatca
    23821 accatggtgg agacagtttt cgaaaatcgt ttccaacacc tagaagagat gcgccgcatg
    23881 ggcttgcatt ctgagattat ccgtgataca gctcgtattg ttggtggaca gcctttgcag
    23941 ggagcagaag ttctttcaac tgaccttcgt gccagtgcag ccttgatttt gacaggtttg
    24001 gtagcacagg gagaaactgt ggtcggtaaa ttggttcact tggatagagg ttactacggt
    24061 ttccatgaga agttggcgca gctaggtgct aagattcagc ggattgaggc aagcgatgaa
    24121 gatgaataag aaatcaagct acgtaatcaa tcgtttactt ttagtcatca tagtactgat
    24181 tttaggtact ctggctctag gaatcggtct aatggtaggt tatggaatct tgggcaaggg
    24241 tcaagatcca tgggctatcc tgtctccagc aaaatggcag gaattgattc ataaatttac
    24301 aggaaattag gctggagaac cagccttttt ctaaagataa ggagaaatat gaacaaaaaa
    24361 acaagacaga cactaatcgg actgctagtg ttattgcttt tgtctacagg gagctattat
    24421 atcaagcaga tgcagtcggc acctaatagt cccaaaacca atcttagtca gaaaaaacaa
    24481 gcgtctgaag ctcctagtca agcattggca gagagtgtct taacagacgc agtcaagagt
    24541 caaataaagg ggagtctgga gtggaatggc tcaggtgctt ttatcgtcaa tggtaataaa
    24601 acaaatctag atgccaaggt ttcaagtaag ccctacgctg acaataaaac aaagacagtg
    24661 ggcaaggaaa ctgttccaac cgtagctaat gccctcttgt ctaaggccac tcgtcagtac
    24721 aagaatcgta aagaaactgg gaatggttca acttcttgga ctcctccagg ttggcatcag
    24781 gtcaagaatc taaagggctc ttatacccat gcagtcgata gaggtcattt gttaggctat
    24841 gccttaatcg gtggtttgga tggttttgat gcctcaacaa gcaatcctaa aaacattgct
    24901 gttcagacag cctgggcaaa tcaggcacaa gccgagtatt cgactggtca aaactactat
    24961 gaaagcaagg tgcgtaaagc cttggaccaa aacaagcgtg tccgttaccg tgtaaccctt
    25021 tactacgctt caaacgagga tttagttccc tcagcttcac agattgaagc caagtcttcg
    25081 gatggagaat tggaattcaa tgttctagtt cccaatgttc aaaagggact tcaactggat
    25141 taccgaactg gagaagtaac tgtaactcag taaaagataa gcctaaactc ctatgtcact
    25201 tatggatgta ggagttcttt ttactagttt aagcagggct agaataggtg ctaagaaaaa
    25261 atagtaactt ctaaaactaa cttccagttt cccacaacct agcttttagt atgagaaaaa
    25321 cgcgtgaaat cagtggaaat ccgtcttaga ctaacttcca gttttgggag agagatggaa
    25381 gttacgctaa gaaaaaaata atatgtattg gattaaattt tgtgatataa taaaggataa
    25441 gatactattt taggagaaga actatggaag acccaagtag tcagaatttg ttgctacaat
    25501 ttgttttatt gtttatcttg acggtattaa atgccttttt ctcagccaca gaaatggcca
    25561 tggtttctct taatcgtgcc cgagttgaac aaaaggcgga agaaggagac agacgctaca
    25621 tccgcctgct gaaggtacta gaaaatccta accacttttt atcaaccatt caggtaggaa
    25681 tcaccctgat tacaatctta tcaggggcga gtttaactga tactctagga cgtgtgattg
    25741 cctcttggct tgggaatggc gaaacagcac aagccgtggc aacttttcta tccttggcat
    25801 ttttgaccta tatttccatc gtttttgggg aattatatcc taagagaatc gctcttaatc
    25861 taaaggatgc cttggcaatt cgtacagcgc cgattatcat tgggatcgga aaactagtca
    25921 gtccttttgt ttggctttta gctgcttcga ccaatttctt gagccatttg actcctatgt
    25981 cattcgatga tgcggatgaa aagatgaccc gtgatgaaat tgcctatatg ctgacaaata
    26041 gtgaagaaac attggatgct gatgagattg agatgctaca aggtgtcttt tcgctcgatg
    26101 aactgatggc acgagaggtt atggtgcctc gaacggatgc ctttatggtg gatattcagg
    26161 atgatagtca agccattatc caaagtattt taaaacaaaa ttattctcgt atcccggttt
    26221 atgatgggga taaggacaat gtaattggaa tcattcacac caagagtctc cttaaggcag
    26281 gctttgtgga cggttttgac aatattgttt ggaagagaat tttacaagat ccactttttg
    26341 tacctgaaac tatttttgtg gatgacttgc taaaagaact gcgaaatacc caaagacaaa
    26401 tggcaatctt gctggatgaa tacggtggta tggctggttt ggtgactttg gaagacctct
    26461 tagaggaaat tgttggtgaa attgatgatg aaacggacaa agcagcaata gatgttcatc
    26521 agattggcga ggatacctac attgttcaag gaactatgac cctcaatgat ttcaataatt
    26581 actttgatgt tgaactggaa agtgatgacg ttgataccat cgctggttat tatttgacag
    26641 gagtgggaac tattccaacg actgagaaac tcagctatga attggtcagc caaaacaaac
    26701 agtttatctt aaccaatgat aaagtgaaga atggacgtgt taccaaggta aaagtccaaa
    26761 tcacagaagt cgaaatagaa gaagaaacag aataaactag aggcttttgt caccaggcgt
    26821 gacgaagctt ttttcaattg atttcaaaag ggaattatgc catggaaaga actctgccac
    26881 aaactcgctc caaaagtttt taaagttata cgtatttatt cgagggaaaa taagaaaagc
    26941 ccttcaaatt gggctttctg ttcatttgaa acctgataaa tcaagttata gaaggcggta
    27001 gacggatttg aaccgacgat caagcttttg cagagccgtg ccttaccact tggctatacc
    27061 gccttaacgt ttattattat accttgaaaa tgatttccag tcaatagttt tttcagtttt
    27121 ttgtaccata gcgtgattcg aatgtgatgc caagttttct atgggaaaaa atttaccaaa
    27181 aacctactga aaccccttga aaaatctagc taaatagggt ataatatggg taatcatttg
    27241 tcgtaggttt tgtctgaaat attgtccaga caaggctcac agcagttaaa tcttctgaaa
    27301 aagtcagatt tagctgctct ttttgtgctt tttttcaaga ttatgagcat ttgtaacaga
    27361 ggcttaaaga ttctgaaaat tcgtcaagag gacacggtga taaggggttt acaaccatat
    27421 gacgattaga aaagcctgat tgacaaggct tggaacttat ttacaaagga gaatcatctt
    27481 ggcaggacat gacgttcaat acgggaaaca tcgtacccgt cgtagttttt caagaatcaa
    27541 agaagttctt gacttaccaa atttgattga aattcaaact gactcattca aagctttcct
    27601 agaccacggt cttaaggaag tgtttgaaga tgtattgcca atttcaaact tcacagacac
    27661 aatggagttg gaatttgttg gatatgaaat caaggaacca aaatacacgc tagaagaagc
    27721 tcgtatccac gatgctagct actcagcacc aatttttgta accttccgtt tgatcaataa
    27781 agaaacaggc gaaatcaaga cccaagaagt tttctttggt gatttcccaa tcatgacaga
    27841 aatgggtact ttcatcatca atggtggtga acgtattatc gtttctcagt tggtccgctc
    27901 accaggtgtt tactttaacg acaaagtaga caaaaatggt aaggtgggct atggttcaac
    27961 tgttatccct aaccgtggag cttggttgga acttgaaagc gactcaaaag atatcaccta
    28021 cactcgtatc gaccgtactc gtaagattcc atttacaacc ttggttcgtg ctcttggttt
    28081 ctcaggtgat gatgaaatct ttgatatctt tggtgacagc gaattggttc gcaacactgt
    28141 tgaaaaagat atccacaaga atccaatgga ctctcgtaca gacgaagcct tgaaagaaat
    28201 ttacgaacgc cttcgtccag gtgagcctaa gacggctgaa agctcacgta gcttgcttgt
    28261 agctcgcttc tttgacccac gtcgttatga cttggcagca gttggtcgtt acaaaatcaa
    28321 taaaaaactc aatgttaaaa cacgtttgct caaccaaacc attgcagagc cattggtaga
    28381 ccctgaaact ggagaaatct tggtagaagc tggtacgatt atgactcgta gcgtgattga
    28441 aagcattgaa agccatttgg atggcgactt gaacaagatt gtctacatcc caaacgatgc
    28501 agccgttgtg actgagcctg ttgttcttca aaaattcaag gttattgctc caactgatcc
    28561 agatcgcgtc gtaacgatca ttggtaatgc taacccagat gacaaggttc gtacggtgac
    28621 tcctgcagat atccttgctg agatgagcta cttcctcaac ttggctgaag gacttggccg
    28681 tgtagatgat atcgaccacc ttggaaatcg tcgtatccgt gcggttggtg aattgcttgc
    28741 caaccaagta cgtttgggac tttctcgtat ggaacgtaat gtccgtgaac gtatgtctgt
    28801 tcaggacaat gaagtcttga caccacaaca aattatcaat attcgtcctg taacagctgc
    28861 agttaaagaa ttctttggtt catcacagtt gtcacagttc atggaccaac acaacccgct
    28921 ttctgagttg tctcacaaac gccgtttgtc agccttagga cctggtggtt tgactcgtga
    28981 ccgtgccgga tatgaagtgc gtgacgtgca ctacactcac tatggtcgta tgtgtccaat
    29041 cgagacacct gaaggaccta acatcggttt gatcaataac ttgtcatctt acggacactt
    29101 gaacaaatat ggttttgttc aaacaccata ccgtaaggtt gaccgtgaaa caggtgttgt
    29161 cacgaacgaa attgtttggt tgacagctga tgaagaagat gaatatactg tagctcaggc
    29221 taactctcgt ctgaatgaag atggaacctt tgctgagaag attgtcatgg gacgtcacca
    29281 aggggtcaac caagagtatc cagctaatat tgttgactac atggacgttt caccaaaaca
    29341 ggtagttgcc gttgcgacag catgtattcc tttcttggaa aacgatgact ccaaccgtgc
    29401 cctcatggga gccaatatgc aacgtcaggc tgtgccattg attaatcctc aggcacctta
    29461 cgttggtact ggtatggaat accaagcagc ccacgattct ggtgcggctg tgattgctca
    29521 gtatgatggt aaagttactt acgcagatgc tgacaaggta gaagttcgtc gtgaagatgg
    29581 ttcattggat gtttaccaca tccaaaaatt ccgtcgttca aactcaggta ctgcttacaa
    29641 ccaacgcact ctcgtaaaag ttggtgatgt cgttgaaaaa ggcgatttca tcgctgacgg
    29701 accttctatg gaaaatggag aaatggcgct tggacaaaac ccaatcgttg cctacatgac
    29761 ttgggaaggc tacaacttcg aggatgccgt tatcatgagc gaacgcttgg tgaaggacga
    29821 tgtctacaca tctgttcacc ttgaagaata cgaatcagaa acgcgcgata caaagcttgg
    29881 gcctgaagaa atcactcgcg aaattccaaa cgttggtgaa gatgccctca aagaccttga
    29941 cgaaatgggg attatccgta ttggtgctga ggttaaagaa ggtgatattc ttgtagggaa
    30001 agtaacacct aagggtgaga aagatctttc agctgaagaa cgtctcttgc acgctatctt
    30061 tggagacaag tctcgtgaag tgcgtgatac ttctcttcgt gtaccacacg gtgccgatgg
    30121 tgtcgttcgt gatgttaaga tctttacacg tgtaaatgga gatgagttgc aatcaggtgt
    30181 taacatgttg gttcgtgttt acatcgctca aaaacgtaag attaaggtcg gagataaaat
    30241 ggccggacgt cacggaaaca aaggggttgt ctctcgtatc gttcctgtag aagacatgcc
    30301 ttaccttcca gacggaactc cagtcgacat catgttgaac ccacttgggg tgccatcacg
    30361 tatgaatatc ggtcaggtta tggagcttca ccttggtatg gcagctcgta ctcttggtat
    30421 tcacattgcg acaccagtct ttgatggagc aagttctgaa gatctttggt caactgttaa
    30481 agaagcaggt atggatagcg atgccaaaac gatcctttac gatggacgta ctggtgaacc
    30541 atttgataac cgtgtttctg ttggagtcat gtacatgatc aaactccacc acatggttga
    30601 cgataaattg cacgcgcgtt cagtcggacc ttactcaact gttacccaac aaccactcgg
    30661 aggtaaagct cagtttggtg gacaacgttt cggtgagatg gaggtttggg ctcttgaagc
    30721 ctacggtgcg tcaaatgtcc ttcaagaaat cttgacttac aagtctgacg atatcaacgg
    30781 acgtttgaaa gcctatgaag ctattacaaa aggcaaacca attccaaaac caggtgttcc
    30841 agaatccttc cgagttcttg ttaaagaatt gcaatctctt ggtcttgaca tgcgtgtcct
    30901 agacgaagat gaccaagaag tggaacttcg cgacttggat gaaggaatgg acgaagatgt
    30961 catccacgta gatgaccttg aaaaagcccg cgaaaaagca gcccaagagg ctaaagcagc
    31021 ctttgaagct gaagaagctg agaaagcaac aaaagcggaa gcaacagaag aagctgctga
    31081 acaagaataa gcagttcact tagaatagaa agggaagaaa tagtggttga tgtaaatcgt
    31141 tttaaaagta tgcaaatcac cctagcttct ccaagtaaag tccgttcatg gtcttatgga
    31201 gaagtcaaaa aacctgaaac aatcaattat cgtaccttga aaccagaacg tgaaggactc
    31261 tttgatgaag tgatctttgg tcctacaaaa gactgggaat gtgcttgtgg taagtacaaa
    31321 cgcattcgtt acagaggaat tgtttgtgac cgctgtgggg ttgaagtaac gcgtacgaaa
    31381 gttcgtcgtg agcgtatggg acatatcgaa ttgaaagctc ctgtatctca catctggtac
    31441 ttcaagggga ttccaagccg tatgggcttg acccttgata tgagccctcg tgccctcgag
    31501 gaagttatct actttgcggc ttatgtggtg attgatccta aggatacacc acttgagcac
    31561 aagtctatca tgacagagcg cgaataccga gagcgcttgc gcgaatatgg ttatggttca
    31621 tttgttgcca agatgggtgc ggaagccatc caagaccttt tgaagcaagt agatcttgaa
    31681 aaagaaattg ctgaactcaa agaagaattg aaaacagcta ctggacaaaa acgtgtcaaa
    31741 gccatccgtc gtttggatgt tttggatgcc ttttacaagt ctggaaacaa acctgaatgg
    31801 atgattctta acatccttcc ggttatccca ccagatcttc gtccaatgtt gcagttggat
    31861 ggtggccgtt ttgcctcatc tgacttgaat gacctttacc gccgtgttat caatcgtaac
    31921 aaccgtttgg ctcgtttgct tgagttaaat gcaccaggta tcatcgttca aaatgagaaa
    31981 cgtatgcttc aagaagcagt tgacgctttg attgacaatg gtcgtcgtgg tcgtccaatc
    32041 acaggaccag gtagccgtcc attgaaatca ttgagccaca tgcttaaagg taaacaagga
    32101 cgtttccgtc aaaacttgct cggtaaacgt gttgacttct caggacgttc agttatcgcc
    32161 gttggtccaa ctcttaagat gtaccaatgt ggtgtgccac gtgaaatggc gattgaactc
    32221 tttaaaccat ttgtcatgcg tgaaatcgtt gcccgtgata tcgtgcaaaa cgtcaaagca
    32281 gctaaacgct tggtggaacg cggagatgag cgtatctggg atatccttga agaagtgatt
    32341 aaagaacacc cagtgctttt gaaccgcgca ccgacccttc accgtttggg tatccaagcc
    32401 ttcgagccag tcttgattga tggtaaggct cttcgcttgc acccacttgt ctgtgaagcc
    32461 tacaatgctg actttgacgg ggaccaaatg gccatccacg taccactttc agaagaagca
    32521 caagcagaag ctcgtatcct catgctagct gctgagcaca tcttgaaccc gaaagatggg
    32581 aaaccggtag ttactccatc tcaggacatg gttttgggta actactactt gaccatggaa
    32641 gaagctggtc gcgaaggtga aggaatggtc ttcaaagacc gtgacgaagc ggttatggct
    32701 taccgcaatg gttatgttca cctccactca cgtgttggta tcgcaacaga cagcctcaac
    32761 aagccttgga cagaagagca aagacataag gtcttgctta caacagttgg taaaattctc
    32821 ttcaacgata tcatgccaga ggggctacca tacttgcaag aaccaaacaa tgccaacttg
    32881 acagaaggtg ttccagctaa atacttcttg ccacttggtg gagatatcaa ggaagctatc
    32941 agcaatcttg agctcaaccc tccattcaag aagaaaaacc tcggaaatat catcgctgaa
    33001 atcttcaaac gtttccgtac gacagaaact tctgccctac ttgaccgcat gaagaacctc
    33061 ggttaccacc actcaactct tgcaggattg acagtgggta ttgccgatat cccagtcgtt
    33121 gatgacaagg ctgaaatcat tgaagaatca cacaaacgtg tcgaacaaat caccaaacaa
    33181 ttccgtcgtg gtatgatcac agacgacgag cgttacaatg ctgttacagc tgaatggcgt
    33241 gctgcccgtg aaaaacttga gaaacgcttg attgctaatc aagatcctaa gaacccaatc
    33301 gttatgatga tggactctgg agcccgtggt aacatctcaa acttctcaca gcttgccggt
    33361 atgcgtggtc tgatggctgc tccgaatgga cgtatcatgg aattgccaat cctttcaaac
    33421 ttccgcgaag gtttgtcggt attggaaatg ttcttctcaa ctcacggtgc tcgtaaaggt
    33481 atgaccgata cggcccttaa gacagccgac tcaggttact tgactcgtcg tttggtcgac
    33541 gttgcccaag acgttatcat ccgtgaggac gactgtggaa ctgaccgtgg tctcttgatc
    33601 cgttctatcg cagaaggaaa agagatgatc gagtctctcg aagagcgtct caacggtcgt
    33661 tacactaaga aaactgttaa acatccagaa actggtgcag tgattattgg tccaaatgaa
    33721 ttgattacag aagacaaggc gcgtgaaatt gtcaatgctg gtgtggaaga agtgactatc
    33781 cgttctgtat ttacatgtaa cactcgtcac ggtgtctgcc gtcactgtta cggtatcaac
    33841 ttggcgactg gtgatgcggt tgaagttggt gaagcagttg gtacaatcgc tgcccaatct
    33901 atcggggaac ctggtacaca gcttacaatg cgtaccttcc acacaggtgg ggttgcctca
    33961 aataccgata tcactcaggg tcttcctcgt gtccaagaaa tctttgaagc ccgcaatcct
    34021 aaaggggaag cggttatcac agaggttaaa ggacaagtta ctgctatcga agaggatgca
    34081 tcaactcgta ccaagaaagt ctttgttaag ggtgaaactg gcgaaggtga atatgtcgtt
    34141 ccatttacag ctcgtatgcg tgtcgaagtt gggggccaag tagcgcgtgg tgctgctctg
    34201 acagaaggtt ctatccaacc aaaacgtctc cttgcagttc gtgatgtctt gtcagttgaa
    34261 acgtaccttc tcggtgaagt acaaaaagtt taccgtagcc aaggggtaga aatcggtgac
    34321 aaacacatcg aggtaatggt tcgtcaaatg atccgtaaag tccgtgtcat ggatccaggt
    34381 gatacagatc ttctcatggg taccctcatg gatatcaatg actttacaga tgccaacaaa
    34441 gatgtcctta tcgcaggtgg agttccagcg acaggtcgcc cagtccttat gggaattacc
    34501 aaagcctcac ttgaaacaaa cagtttcttg tcagcggctt ccttccagga aacaactcgt
    34561 gtccttactg acgcagctat ccgtggtaag aaagaccatc tccttggact taaagaaaat
    34621 gttatcatcg gtaagatcat cccagctggt actggtatgg cccgttaccg taaccttgaa
    34681 ccacatgctg tcaacgaaga agaatacctt aatcctccag tagaggaaga aggaaatgaa
    34741 gaaacaacag aagtagttgt ggatactgcc gttgaaactg tggaagaaac agtagaataa
    34801 gagagaatga gagaaccctc gggttctctc attctctctt ctgaaaactt tctccatttt
    34861 tccacgaaat gtggtaaaat aaaagaaaga agctgacaaa ggagacgggt atggaacaaa
    34921 cattctttat catcaaacca gatggtgtaa aaagagggct agtgggtgaa gtgttaaagc
    34981 gcatcgaaca acgtggattt acaatcgaaa aattggagtt tcgttcacag gtttcagaag
    35041 agttgattgg ccagcactat caggacttgg ttggtcagag tttttaccca ccgattcgtg
    35101 aattcatgac ttcaggtcca gttcttgtgg gtgtcatttc tggtcccaaa gtaatcgaaa
    35161 cttggcggac catgatgggt gcaactcgtc cagaagaagc tttaccaggc actattcgag
    35221 gtgattttgc aaaagctgct ggagaaaatg agattatcca aaatgttgta catggttcag
    35281 attcagaaga gtcagctaag cgagaaattg ctctttggtt ttaagagtgg attggctcaa
    35341 tcaattggtt aaaagctcat ttgaatagaa agtatagtca attagtttaa gacatgacgc
    35401 atgatatcaa actttttagt ttttgatatg gtgcgttttt tgattaagtg actattagtt
    35461 tgtctcgctc cgttaggtgc gagacaaaaa aaacacccgc tatgcgggtg cgcgtcgaag
    35521 gttataccaa aaaaactcca aacgcgatac aataaaggtg ttcaatccaa ttgtaaagcg
    35581 aaaggagaaa aatatggcac aaaaggttct tttatcctgt tcttgtttca attgactata
    35641 tttctatact ttttcttcct ttctctgtga aaatcgcaag tttttcccac cttttctcaa
    35701 tactgaaaca acatctttcc agaacttcaa taatttcttt ttgtgttaaa tcattattta
    35761 actaaatctt tggaggcaat acgttttcaa agctactttt ttaccatagg ttttgagcct
    35821 tcaacacaga ttactaagcc aaaaatgctc taaatttttg aaattctaac aaattccact
    35881 caactttatc atcatctttg actttcacaa tatcttcctc aaaataacat ggaataaaga
    35941 tagttttctt ttaaaacggt tgtaggagga aaatctctcc tacactatta ttgattctga
    36001 gagaaaattg caaaatatca tctaccaaac tttcaattat taagattttt tgcagtttat
    36061 ctgaattaga atcgcaaggc ttctcaataa tataattgtc gaattttttc attctaactt
    36121 cctctccatt atttcttcct ttatcaagaa aaagttacct caatattaat aaagtacaat
    36181 tctaaattaa ctccaattct tgattcatga taatatttca gtaactcatc gtgcaattaa
    36241 aaacaaggat ttttatttca gatgatccat cgtgaacact tcatcagcca tctcccaaat
    36301 tgccggatta tgtgttgcga taatgattag cctattatca tctcgaagag atagcaaaat
    36361 ctccataatc aactgagagg ttgctgggtc tattgaagct gttggctcat ctgccagaat
    36421 aaagggtgga ttctttaaga taatttttgc caaggcaacc cgttgcgatt ctccgcccga
    36481 taactcaaag atgcgcttat ctaggtcaag ataagccagg ccgacctgtt ctaaagcctg
    36541 cttctgcctc aaccgctgtt ccgaccgact caacttttga ccaatgagac ctagcttaag
    36601 gttttcttca atactttggt tttcaattaa gccaaagttc tggaagaggt agcccaattc
    36661 gtgacggaaa aaatcactcg atttataatt ggccaagtct ttacctcggt aaaaaatcgt
    36721 cccatcataa ggttctaatt tcccaatcat gttcatcaag gttgtttttc cgctaccact
    36781 tgaaccaatt aaggcataga cttttccagc ctcaaatgtc attgaaagat tcgaaaataa
    36841 ctctcgttct ccaaaagatt tactcacctg tttaagttca atcatattaa cctcccttca
    36901 aaacaagaat ggacatcttg ttttctttct gcatttggac atgtaactgt aaaagagata
    36961 gaccagtaaa gagtaacaag actaagaaag caactcctat ctctacctga agaaatacac
    37021 tcgcaacaaa tcccagtaaa aacacaccca gttgagcaaa gagataagtg cgatggattt
    37081 ctaagaacct gagacctgca atgcgtttga taaaaatggc acgtctaaat tcttcaaagt
    37141 agagcctatt catagtgtta aacaacaaga ttgaagttgc aatcccaagc acagctcctg
    37201 ctagcattac ccaacgttcc ctctggatat tatccaataa tgtgatgtag ttgtggtaac
    37261 ctgtttgcat ttctgagacc caattttcaa tgccttgtct ctggataagc tcctgggcat
    37321 cagacaattg attaaagaga acgtagttct gtactgcgtc tatccaaaac aaaatggact
    37381 gtggaccagt tgattggggt gttataacaa tgatgattgg atctttcaaa aactgctggt
    37441 aagaaatagg ggtcgtatta tacacaaaac gatcctgacc tgattctaaa taacctaccg
    37501 tagcagtcat ttgctgtcgt tcatcttgac tagacatgcg actggttaag tcgtcttcaa
    37561 aaacagattt ataatgttct tcctctgaac ggaggtgttc aggcagcaat aagacaaact
    37621 cccctacatt aaggtgattc atcttttgct caatagttgt atctacagga atgttttgac
    37681 gctccaagta ttgcggtgtg acgataagga cattgccatt cgggttgtaa tcgtgccatt
    37741 ctgtagaggt tatcaagttt ttggaggaag ccatgccatt ttgcaaagta cggtcaatta
    37801 actggtgtct agataagaaa gccttttgtt ctgaaacagc cagatccatc aattgatacc
    37861 aagttctgag tttcctttga gcttgttcat caaaaccagg actcgttcct tcacggctga
    37921 ttgatagggt aatcagttgc ctttcctgac tccaagcctc ttgtccaatc cgatgctgtt
    37981 gccaggcttg ggaatacttt aagctactcc ctattcccag cgttacaata ataatcgcta
    38041 atagttgacc gattaaaatc aaactaatga ttcctctgac aggcacttgc ccttttataa
    38101 tctgcatcag gtgtatcttt tttatcccaa ctgcgaagag ttgagcgaaa aacagggaga
    38161 tcgacaacaa gatgagatta taactgagca agcctgctcc tatggtcatt agggattgcg
    38221 ttggaagcga cagaattttt tgcataagaa tggcgagcac gccagctaaa ctgaaaccaa
    38281 cagctatccc tttcaaatcc tcaccaactg gtctaaggaa aatggaccac cgtttctctc
    38341 ctgaaatgag gcgaatgccc gacgaccgta attggctaat ttgactaatt aaagtcagtg
    38401 ctacaaaggt taagatgaaa atcaatagac tgattaactg aaatccgtta ctaaagatag
    38461 ccattaacgt agatagttta gatggaattg tcaggttcat gttagtcaag ccaagttgac
    38521 ttagctcctc ccttagcaag tgaatatcta ggtgtccatc gaatacaaaa tagtttgttt
    38581 caacactact actttgagca tcttctagtt ttttttcctg aagcccatca ggcaactttc
    38641 cgtccccaaa ggtcgtataa gaaaaaactg tagtaccttc tgagttagaa tcttggtgtt
    38701 gaatcgcaat aaagctatct gtttcttctg ctagtttttc caagcgtgta gcaacatgct
    38761 gaaaagttgt ttcaggggaa gaatcacgta caacaacgtt ggggtaataa tgggagacat
    38821 atgtatcagt ccaaatggta aatacccaaa caaagaacaa actagcaagc aggttggata
    38881 tgagtataaa taattttttc atagtgcatt ccttattcta aaacagaggg cagaaatacc
    38941 cctgccctct ataaactaac aagcttacta aactattgat aaaagaatct tcttcaatta
    39001 ttctttacca tcccgcatca aatgtaacta attttttaat tccacgtttt tttctcatgc
    39061 aatgaaaccc ctttcttttt ccttgttttt at
//
LOCUS       DAVKLW010000014        37797 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKLW010000014 DAVKLW010000000
VERSION     DAVKLW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.014"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(75..1094)
                     /gene="adhP"
                     /locus_tag="VW324_000701"
     CDS             complement(75..1094)
                     /gene="adhP"
                     /locus_tag="VW324_000701"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9726376.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(1433..2245)
                     /locus_tag="VW324_000702"
     CDS             complement(1433..2245)
                     /locus_tag="VW324_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9726377.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            2435..3853
                     /locus_tag="VW324_000703"
     CDS             2435..3853
                     /locus_tag="VW324_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9726378.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            3906..4613
                     /locus_tag="VW324_000704"
     CDS             3906..4613
                     /locus_tag="VW324_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9726379.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            4708..5682
                     /gene="folP"
                     /locus_tag="VW324_000705"
     CDS             4708..5682
                     /gene="folP"
                     /locus_tag="VW324_000705"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9726380.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGKFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            5684..7006
                     /locus_tag="VW324_000706"
     CDS             5684..7006
                     /locus_tag="VW324_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9726381.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            6987..7541
                     /gene="folE"
                     /locus_tag="VW324_000707"
     CDS             6987..7541
                     /gene="folE"
                     /locus_tag="VW324_000707"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9726382.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            7584..8396
                     /gene="folK"
                     /locus_tag="VW324_000708"
     CDS             7584..8396
                     /gene="folK"
                     /locus_tag="VW324_000708"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9726383.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNRGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(8441..8758)
                     /locus_tag="VW324_000709"
     CDS             complement(8441..8758)
                     /locus_tag="VW324_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9726384.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            9113..9559
                     /gene="rplM"
                     /locus_tag="VW324_000710"
     CDS             9113..9559
                     /gene="rplM"
                     /locus_tag="VW324_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9726385.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            9578..9970
                     /gene="rpsI"
                     /locus_tag="VW324_000711"
     CDS             9578..9970
                     /gene="rpsI"
                     /locus_tag="VW324_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9726386.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(10231..10653)
                     /locus_tag="VW324_000712"
     CDS             complement(10231..10653)
                     /locus_tag="VW324_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9726387.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            10800..10976
                     /locus_tag="VW324_000713"
     CDS             10800..10976
                     /locus_tag="VW324_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726388.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            11377..13590
                     /locus_tag="VW324_000714"
     CDS             11377..13590
                     /locus_tag="VW324_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9726389.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(13812..14288)
                     /locus_tag="VW324_000715"
     CDS             complement(13812..14288)
                     /locus_tag="VW324_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9726390.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQR
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            14511..17714
                     /locus_tag="VW324_000716"
     CDS             14511..17714
                     /locus_tag="VW324_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9726391.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRISYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            18094..18479
                     /gene="tnpA"
                     /locus_tag="VW324_000717"
                     /pseudo
     CDS             18094..18479
                     /gene="tnpA"
                     /locus_tag="VW324_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(18858..19487)
                     /locus_tag="VW324_000718"
     CDS             complement(18858..19487)
                     /locus_tag="VW324_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9726392.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(19497..20498)
                     /locus_tag="VW324_000719"
     CDS             complement(19497..20498)
                     /locus_tag="VW324_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9726393.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(20529..21170)
                     /locus_tag="VW324_000720"
     CDS             complement(20529..21170)
                     /locus_tag="VW324_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9726394.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(21189..22004)
                     /locus_tag="VW324_000721"
     CDS             complement(21189..22004)
                     /locus_tag="VW324_000721"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9726395.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGEANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            22275..22709
                     /locus_tag="VW324_000722"
     CDS             22275..22709
                     /locus_tag="VW324_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9726396.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            22721..23911
                     /locus_tag="VW324_000723"
     CDS             22721..23911
                     /locus_tag="VW324_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9726397.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            23922..24413
                     /locus_tag="VW324_000724"
     CDS             23922..24413
                     /locus_tag="VW324_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9726398.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            24428..25207
                     /locus_tag="VW324_000725"
     CDS             24428..25207
                     /locus_tag="VW324_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9726399.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            25194..26012
                     /locus_tag="VW324_000726"
     CDS             25194..26012
                     /locus_tag="VW324_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9726400.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            26012..26305
                     /locus_tag="VW324_000727"
     CDS             26012..26305
                     /locus_tag="VW324_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9726401.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            26327..28228
                     /locus_tag="VW324_000728"
     CDS             26327..28228
                     /locus_tag="VW324_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9726402.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLIDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            28288..29289
                     /locus_tag="VW324_000729"
     CDS             28288..29289
                     /locus_tag="VW324_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9726403.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(29399..29734)
                     /locus_tag="VW324_000730"
     CDS             complement(29399..29734)
                     /locus_tag="VW324_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726404.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(29745..29987)
                     /locus_tag="VW324_000731"
     CDS             complement(29745..29987)
                     /locus_tag="VW324_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726405.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(30003..30197)
                     /locus_tag="VW324_000732"
     CDS             complement(30003..30197)
                     /locus_tag="VW324_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9726406.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            30363..31313
                     /gene="rsmH"
                     /locus_tag="VW324_000733"
     CDS             30363..31313
                     /gene="rsmH"
                     /locus_tag="VW324_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9726407.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            31325..31642
                     /gene="ftsL"
                     /locus_tag="VW324_000734"
     CDS             31325..31642
                     /gene="ftsL"
                     /locus_tag="VW324_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9726408.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            31646..33898
                     /gene="pbpX"
                     /locus_tag="VW324_000735"
     CDS             31646..33898
                     /gene="pbpX"
                     /locus_tag="VW324_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9726409.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            33900..34880
                     /locus_tag="VW324_000736"
     CDS             33900..34880
                     /locus_tag="VW324_000736"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9726410.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            35489..37594
                     /locus_tag="VW324_000737"
     CDS             35489..37594
                     /locus_tag="VW324_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9726411.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLAAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGGLVIREKS"
BASE COUNT        11428 a         6814 c         8429 g        11126 t
ORIGIN      
        1 ttgaattctt atttaaaatt taatataaaa aagttggaag aagcttccaa cttttaaaat
       61 aggtttctat tagattagtg ggtgaagtcg agtaccatac gtccttggat ttggcctttt
      121 tccatttcgt cgaaaatggc aacagcatct tctactggac gtttttgaac aactgggact
      181 accagacctt ctgcaccaaa ttggaaggct tcttctaagt ctttacgagt tccgacaaga
      241 gaaccgatga cttggattcc atcgaggact gttttaacga tgctgagttc catcatttca
      301 gaaggaagac caacagcgac gacgcgacca ccagcacgaa tggagtcaac agcctggttg
      361 aaggcaactt tagacacagc agttacgaca gctgaatgag ctcctccatc agttttttct
      421 ttaatgagtc cagctacatc ttcaacttcg aggccgttaa tcacaatgtc agcgcctact
      481 tcttttgcaa gggcaagttt gtcattgttg atatcgactg cgataacatg agcattgaat
      541 acttttttag cgtattgaac agcgaggtta ccaagtccac cagcaccgta aagaacaacc
      601 cattggcctg gttcaacttt tgcttctttg atagctttat aggttgttac tccagcacat
      661 gtgatagaag aagcttgggc tggatcaagt ccgtcaggaa ctttgacagc atagtcagca
      721 gttacgatac attgttcagc cataccaccg tctactgagt agccagcatt tttcactgta
      781 cggcaaaggg tttcgcgacc agttgtacag tattcgcaag tgccacatcc ttcaaagaac
      841 caagcaacgc tgacgcggtc accgacttta aggcttttca catctggagc aatctcttta
      901 acgataccga taccttcgtg cccaagaaca cgtcctggga cttgaccaaa gtcaccatga
      961 gcaacgtgga ggtcggtgtg gcaaacacca cagtattcaa cttctacaag tgcttcccca
     1021 gtttcaagtg gacggagtac tttttcttca atagcaacac cagtgctttc tggatttaca
     1081 acaacagctt tcatagctat cctccttgat attgaccgag aacagggaca acgggactgg
     1141 attgattttt tgtcaacaga gtcgagtgtg acgagctctc ttgtatttat atgtgaagta
     1201 tatcacaaaa gaaagccttt tccaaggttt tggagacaaa aagtagaaca atcgattaac
     1261 tggttgatga gactgtgaaa agagcacaaa gaccagcttg caagctggtc atactcttcg
     1321 aaaatctctt caaaccacgt cagcttcatc tacaacctca aagcagtgct ttgagcaacc
     1381 tacggctagc ttcctagttt gctctttgat tctcattgag tatcagtata ggttataaga
     1441 ataagtcttg tagggttttg gcaaccccat cttggtcgtt ggtaagggaa atttgctcat
     1501 ctgcataagg gagtagctct ggattggcat ttttcatggc ataacccttc ccagcaaaag
     1561 cgagcatttc ggtatcattg tgttcatctc caaaggcaat caaatctttt ttgtcacgat
     1621 tcattatctt gagcaagtag tccaaagcaa aggccttgtt gacacctttt ggggtacatt
     1681 caaggatatt gagcggacct ccccaggtat tgatagaaag ttgatgctgg tagaaggcgt
     1741 tcatgtcttt tgccaaggca tatttgtcac tggctctggt ctgcaagagg atacagttag
     1801 ggtccttggt caccaattca ggctggaatt gatcttcagg ctggaaagct tctacaccaa
     1861 atagtttggg attggcaatt tcttcattgg gatttgtaat gtagaatttt ttacgatatt
     1921 ctccagcgat aaaatcggct tgaatgtcct ctgaacgttg aaccatatct agcagatatt
     1981 ttttgtctac agtcaaacac ttttcaaaat cccaaacttg gtctggtaaa tgagtaaggg
     2041 atccgttgaa gttaatcata ggagtgtcta agcccagttc acggtaaaaa tcttttgaca
     2101 tacggtaagg gcgacctgtc gtaataataa cctgatggcc tttttcagca acttttttga
     2161 tggttctttt ggtaaagtca gaaatttgac tgtctgagtt gagcagggtt ccatccaggt
     2221 caactgcaat aatttttttc gtcatatgga cctttctagt gtgttttcag ataagatgtc
     2281 ttctattata acagaaagca ggcagaaaag cagattccaa acaaaaaatg aaattggatt
     2341 aatttcttaa acaaatcaaa caaagacatt gaaaaagtga atgataaatg atataattct
     2401 attattgttc gtaaaaatta aaaggagatt gatgatggac aaattattta aactaaaaga
     2461 gaacggtaca gacgttcgta cagaggttct cgctggttta acaactttct ttgcaatgag
     2521 ctatattctc tttgtaaacc cacaaatact ttcacaaaca ggaatgcctg ctcagggcgt
     2581 cttcctagcg acgattattg gtgcagtagc gggtaccttg atgatggctt tttatgctaa
     2641 cttaccttat gcccaagcgc caggtatggg actcaatgcc ttctttacct ttacagttgt
     2701 attcgggctt ggttattctt ggcaagaagc cctagctatg gtcttcatct gtgggattat
     2761 ttcattgatt attaccttga caaatgttcg taaaatgatc attgaatcga ttcccaatgc
     2821 tcttcgctca gctatttcag ctggtatcgg tgtcttcctt gcctatgtag ggattaagaa
     2881 tgctggactt ttgaaattca cgattgatcc aggcaactat actgttgtag gagaaggggc
     2941 tgacaaagct caagcaacga ttgcagcaaa ctcttcagca gttccaggat tggtcagctt
     3001 taataatcca gctgttttag tggctcttgc aggacttgcc attactatct tctttgtcat
     3061 caaagggatt aaagggggaa ttattctctc tatcttgaca acaactgttc ttgctattgc
     3121 agttggtttg gttgatttgt ctagtatcga ttttgctaat aaccatgttg gtgcagcttt
     3181 tgaagatttg aagacaatct ttggtgcagc tcttggttca gaaggtttgg gagctttggt
     3241 ttcagataca gctcgcttgc ctgaaactct gatggccatt cttgccttct cattgacaga
     3301 tatttttgac acaattggta ccttgatcgg tacaggtgaa aaagttggta ttgtagcgac
     3361 aaatggtgaa aatcaccaat cagccaaatt ggataaggct ctttactctg atttgattgg
     3421 aacgacagtc ggtgccattg caggtacttc aaacgtaacg acttatgttg agtctgctgc
     3481 tggtatcggt gcaggtggac gtactggttt gacagccttg gttgtagcta tctgttttgc
     3541 gatttcaagc ttctttagcc cacttctagc gatcgtacca acagcggcta cagctccaat
     3601 cttgattatc gttgggatta tgatgcttgg tagcttgaaa aatatccatt gggatgatat
     3661 gtctgaagca gttcctgcct tcttcacatc tatctttatg ggattcagct actctatcac
     3721 tcaagggatt gcagttggtt tcttgactta cactttgact aagcttgtca aaggtcaagt
     3781 taaagatgtt catgccatga tttggatttt ggatgccttg tttatcctta actacatcag
     3841 catggcctta taatagaatg acccaggggg attttccccc ttttttaata caaaggagat
     3901 aggtgatgaa agagaaaaat atgtggaaag aattgttgaa tcgtgcaggc tggattttgg
     3961 tctttttact tgccgtcctt ttatatcagg ttcccctagt ggttacctct attttgactt
     4021 taaaagaagt agccctgcta cagtcagggc tgatagttgc tggcctttca attgtggttc
     4081 tggctctatt tattatggga gctcgtaaaa ccaagttagc tagttttaat ttttcttttt
     4141 ttagagctaa agatttggca cgtttgggct tgagttattt agttattgtc gggtcaaata
     4201 tacttggttc tatcttgttg caactgtcaa atgagacgac aacagctaac cagtctcaga
     4261 ttaacgatat ggttcagaat agttctctga tttctagctt cttcttgcta gccttgcttg
     4321 ctccaatttg tgaggaaatc ttgtgccgtg ggcttgttcc taaaaagatt ttccgaggca
     4381 aggagaactt gggatttgta gtcggtacga ttgtgtttgc tttattgcat caaccaagta
     4441 atttaccttc tttattgatt tatggaggta tgtcgacagt tctatcttgg acggcctaca
     4501 agacccaacg tttggaaatg tcgatcttgc ttcacatgat tgttaatggg attgctttct
     4561 gtttgttggc tcttgtggtg attatgagtc ggacattagg aatttctgtg taaaagtttt
     4621 tatgtaggaa ccgacctctt tctactaggg aaagatgaat gcaatcgtgt ccatcttttt
     4681 ctttttatgg taaaatagaa aaataatatg atgaaaatcc ttgagggagt gaccgatatg
     4741 tcaagtaaag ccaatcatgc aaagacagtt atttgcggaa ttatcaatgt aaccccagac
     4801 tccttttcgg acggtggtaa attttttgct cttgagcagg cactccagca ggctcgtaaa
     4861 ttgatagcag aaggagccag tatgctcgat atcggcggag aatcgactcg gccgggcagt
     4921 agctatgttg agatagaaga ggaaatccag cgtgttgttc cagtgatcaa agcgattcgc
     4981 aaggaaagtg atgtcctcat ctctattgat acttggaaga gtcaagtagc agaggctgct
     5041 ttggctgctg gtgccgatct agtcaatgat atcactggtc ttatgggtga tgagaaaatg
     5101 gctcatgtgg tagctgaagc gagagcgcaa gtggtcatca tgtttaatcc agttatggcg
     5161 cgacctcagc accctagctc gctcatcttc cctcattttg gttttggtca agcttttaca
     5221 gaggaagagt tagctgactt tgaaacattg ccaatcgaag aattgatgga ggctttcttt
     5281 gaacgagcac tagcgagagc ggcagaagct ggtattgcac cagaaaatat cctgttggat
     5341 ccaggaattg gctttggtct gaccaagaaa gaaaatctgc ttcttttacg ggacctggat
     5401 aaactacatc agaagggcta tccaatcttt ctcggagtgt cgcgcaagcg atttgtcatc
     5461 aatatcctag aggagaatgg ttttgaagtc aatcctgaga cagagcttgg tttccgaaat
     5521 cgggacacgg cttcggctca tgtaactagt atcgctgcga gacagggtgt agaagtggtg
     5581 cgcgtgcatg acgtagctag tcacaggatg gcagttgaaa ttgcctctgc cattcgtctg
     5641 gctgatgaag cggaaaattt agatttaaaa caatataaat aagatgaaag aaattgaaaa
     5701 caatcagtgg attgctaact accggacgga tcaaccgcat tttggattgg aacgaatggt
     5761 ggaactgtta gctttgcgtg gcaatcccca tctcaaactc aaggtcctcc atatcggagg
     5821 gactaacggc aagggctcga ctattgcttt tttgaaaaag atgctagaaa agctagggct
     5881 gagagttggc gtgtttagct cgccctatct cattcattac acagaccaga ttagcatcaa
     5941 tggggaatcg atctcagaag cgaggctaga agctctcatg gcagactatc agtctttgct
     6001 ggagggagaa gcggtcgcca atttacaggg cacaaccgag tttgagatta tcacagccct
     6061 ggcctatgac tactttgcct cagagcaagt agatgtggcc atcatggaag ttggcatggg
     6121 tggacttttg gatagtacca atgtctgtca gcccattttg acaggaatta caactattgg
     6181 cttggatcat gtggccctac ttggtgacac cttggaggcc atagcagagc agaaggcagg
     6241 tattatcaaa caagggatgc ccttggtaac agggcgtatt gctccagaag ccttgactgt
     6301 gattgaccgc attgcggaag ggaaagatgc gccgagactt gcctacggga cagattatca
     6361 ggttcgtcat caagaaagtg tggtgacagg ggaagtcttt gactatacaa gtgctgtcag
     6421 acaaggtcgc ttccagacta gcctgcttgg tttgtaccaa atagagaatg ctgggatggc
     6481 catagcttta cttgatactt tttgtcaaga agatggtcga gagctagcaa gcaatgactt
     6541 tcttggtcaa gccttggaag aaacaagttg gccagggcgt ttggaaatcg tgtcaagaga
     6601 tcccttgatg attttggatg gagcccataa tccccatgct atcaaggcct tgttggtaac
     6661 cttgcaagaa cgttttgcgg attatcataa ggaaatcctc ttcacttgta tcaaaaccaa
     6721 agccttggag gatatgttgg acttgctggg agccatgcca gataccgagc ttactctaac
     6781 acattttgcg gatagtcggg cgacggatga aagcgtgctg aaagaggcag ctaagtctag
     6841 aaatctcagc taccaagatt ggcatgattt tctagagcag aatgtgacag ataaaaaaga
     6901 agagaaacaa acagttagga ttgtcacagg ttccttgtat ttcttgagcc aagtgagggc
     6961 ctatctgatg gagaggaaga acgagaatgg atacacaaaa gattgaagca gctgtaaaaa
     7021 tgattatcga ggctgtagga gagaacgcta atcgcgaggg cttgcaggaa acacctgctc
     7081 gtgtagcccg tatgtatcaa gagatttttt caggtcttgg tcaaacagca gaggaacatt
     7141 tgtcaaaatc ctttgaaatt attgacgata atatggtggt agaaaaggat atctttttcc
     7201 ataccatgtg tgaacaccac ttcttgccat tttatggtag agcgcacatt gcctacattc
     7261 cagatggtcg tgtggcaggc ttgtctaagc tagcccgtac ggttgaagtt tattcgaaaa
     7321 aaccacaaat tcaagaacgt ttgaatatcg aagtggccga tgccttgatg gactatctag
     7381 gtgctaaagg agcctttgtt gtcattgagg cggaacatat gtgtatgagt atgcgtggtg
     7441 ttagaaaacc aggcactgca accttgacga cagtagctcg tggtctattt gaaacagata
     7501 aggacctccg cgaccaagct tatcgtttaa tggggctata aaaagaatcc gcttcaagcg
     7561 gatttttcta gaaaggaatc attatggatc aactgcagat taaggatttg gaaatgtttg
     7621 cctatcatgg tctttttcct agtgagaaag aattggggca gaaatttgtc gtttcagcca
     7681 tcctatccta tgatatgacc aaggcagcta cagacttgga tttaacagcc tctgtccatt
     7741 acggagaatt gtgtcagcag tggacgactt ggtttcagga aacgagtgaa gatttgattg
     7801 aaacggtagc ctataaactg gtggaacgta cctttgagtc ttatcctctt gtccaagaaa
     7861 tgaagttgga actgaaaaaa ccttgggcac cggtgcattt gtcactagat acttgctcgg
     7921 taaccatcca tcgccgcaag caacgagcct ttatcgccct aggaagcaat atgggagata
     7981 aacaagcaaa cttgaaacaa gccattgaga aaatgcgaaa tcgaggtatc catattctca
     8041 aagagtccag tgtcttagcg acggagcctt ggggtggagt ggagcaggat agctttgcca
     8101 atcaagtggt tgaggtggaa acctggctac cagcacaaga cttgttagaa accttgttag
     8161 ccattgagtc agagctggga cgggtgagag aagtgcattg gggacctcgt ttgattgatt
     8221 tggacttgct ctttgtggag gaccagatcc tttatacaga cgacctcata ttgcctcatc
     8281 cttacatagc ggaacgcctt tttgtccttg agtccttaca ggaaattgcg cctcatttta
     8341 tccatccgat attaaaacaa ccgatccgca acttgtatga tgctttgaaa aaatagaaaa
     8401 actctagttt tcagttactt gcaactgaag gctagagttt ttaaaatagg tcattttctt
     8461 ctgggaggag gatagtttct ctaccgtcca tgtctaaaac cagtactctt gggggataac
     8521 gagggtcgaa aggatggtta aagtcaaaat caatggctgt agggaggtgt tgacttgaaa
     8581 agtggaaggt aatctttcct tggttattaa gcaattgaaa ctcgagttct tcttccaatt
     8641 caaagacatt ttttaagaaa tgatcgatga tataccaaaa agagtcaatg atgtcatcgg
     8701 gcaagctggt aacaatgcca aaactagcag accgcatgtg ggtattggta aaagccatat
     8761 ctctgtcccc tttcttttcc cttatcatac agcaaatagg attaaaaatc aagaaaaggt
     8821 gattttttga aaaggatttt agttacaggg agaaataggg aaaaattccc aaaaatctac
     8881 cgaagttaat aggtaaattc ccaaattaac ttgattatat aactttcagt tattttgaga
     8941 agttaccgaa aaatattttt tatatctatt gacttttagg ggtgaaattt ggtatgatag
     9001 taggcggtat tgtttacccc atttgaaagg ccccggaacc ttccaaatac ttttcgatgg
     9061 gaaggaacac ccatcaccgt aaacaaaaat cgaactatat ataggagaaa tcatgaacaa
     9121 aacaacattt atggctaaac caggccaagt tgaacgtaaa tggtacgtag ttgacgcaac
     9181 tgatgtacca cttggacgtc tttctgcagt agttgctagc gtacttcgcg gaaaaaacaa
     9241 accaacattt acaccacaca ctgatacagg tgacttcgtg attgttatca atgctgaaaa
     9301 agttaaattg actggtaaaa aagcaactga taaaatctac tacactcact caaaccaccc
     9361 aggtggattg aaacaaatct ctgcaggtga acttcgttct aaaaatgcag tacgtttgat
     9421 tgagaaatca gttaaaggta tgcttccaca caatacactt ggacgcgctc aaggtatgaa
     9481 gttgaaagta tttgttggag ctgagcacac tcacgctgca caacaaccag aagttcttga
     9541 catttcagga cttatctaag gaaaggaaca ataaagtatg tcacaagcac aatatgcagg
     9601 tactggacgt cgtaaaaacg ctgttgcacg cgttcgcctt gttccaggaa ctggtaaaat
     9661 cactgttaac aaaaaagatg ttgaagagta catcccacac gctgaccttc gtcttgtcat
     9721 caaccaacca ttcgcagtta cttcaactgt aggttcatac gacgttttcg ttaacgttat
     9781 aggtggtgga tacgctggtc aatcaggagc tatccgtcac ggtatcgctc gtgcccttct
     9841 tcaagtagac ccagacttcc gcgattcatt gaaacgcgca ggacttctta cacgtgactc
     9901 acgtaaagtt gaacgtaaga aaccaggtct taagaaagct cgtaaagcat cacaatttag
     9961 taaacgttaa ttcgaaagaa ttactatact tatacagagc acctttcggg gtgttctttt
    10021 ttatactttc ttactaaatt ggtgcaattg acacagttgt tgcgacttta gtcgcttaca
    10081 aatgtggctg caacctgaca aggtcagttg cctcaaaacg ttaatcaata cgattatatc
    10141 aacgtttcaa agcactcaag agtttacctc atgggtgctt tttttatgtt ttttgaaaaa
    10201 gttcacctca aagtttacct tattttcacc ttattgtttt agagactgta aggcaaattc
    10261 accgacggcc atagggacta cattagaagt attgacataa atctgtgttg tacctgtgtc
    10321 gctatgtatt agagcttcag aaatggcttc taagctcatt cctgcttgtt tagcgagcgt
    10381 tgctccggta tgacgtagtt tgtgtggcgt agcgtgtgta agctctgggt gtcgttttct
    10441 gatagttttc atcttattat tgagatagtc agaatgtaaa ggtttattga tattgccttt
    10501 ggtatctatg taagtaaaga tgaattgttc tggattactg atgataccaa actttgccag
    10561 ttcatatttt tgttgttttt tccatgaagt cagaagttga agtaagtcat tagaaataga
    10621 gaaaatggga aatgtttcca caataaaacg catattatca gatggttaat gcgttttata
    10681 tttgggtttt aggcttataa aataggataa gcgtactgaa aacttagata tgacaaacaa
    10741 aataaaattt ttaaaagcta cagagaaaca tggtataatg tatctaaagg aggtagcata
    10801 tgtccttaga tatagataaa gaaaagatga caatcatggg aatagctttt gaaaaccgat
    10861 ccgtttttaa gagtgtttgg tatgctttaa gtacaaatat gattgaagga tggcgaccta
    10921 cggttagtga tgttgaaaaa ttacgagatg aagctcttgc tctggggatg acttaatgaa
    10981 gcgcaactgt tatgagtctt atgactatgt tgatccggat aatttatata ccgatccggg
    11041 ttcttcagtt ctaagaaata agcaggaagg aaaaaagcac gcgagcttga atatcgtatg
    11101 attgctagta agagtttgaa attatttatc aatcctatct tagttaatag tttaaaagta
    11161 gtttgtttca caactgcata agcttagaaa agttgaaaag tgataactct ttaataaact
    11221 cttggtttgt taaaagatat tttgtccact acattttttc aattgactat tatttgtcat
    11281 agatataaca gatagaacat tgcattcaga ataatgaaaa acgcatcaaa aatggtgcgt
    11341 tctgttttta tgctaccata aaaggaggaa aacaaattga aaatttttaa gggagagttt
    11401 tatcgaatct ctgtattaac agacaagcta gtaaggttag aatactctca aactggaagt
    11461 tttgaggata gaacgacaca acttatctat aatagagatt ttggccaagt ttcgttagat
    11521 tatatcgaga catcaaacgt actagatatt atgacggact attttcatct gcactttaat
    11581 aaaggagaat ttaacgccga aaatttattt atagaattaa aaggaaattt tgccgtatat
    11641 ggtagtcgct ggtattttgg tgaatctatt gaaacgttaa aaggaacagc tcggactctg
    11701 gataaggcag atggagcaat ctcgttagaa gatggaatta ttagccgaaa tggtatagcc
    11761 ttattggatg attctcaagg atttatttgg gatgaacaat ctggttatat tgagagagaa
    11821 aatcaaattg acctgtattt ctttgcctat gggcatgatt atagaggagc aatcagagac
    11881 ttttaccatt tgactggttc aacacccttg ttgccaagat atgctttagg caattggtgg
    11941 agtagatatt ggccttatac gtcggatgaa tacttggatt taatagacag atttgcaaca
    12001 gagaaaattc cattatctat cggtgtgtta gatatggatt ggcatataac tgacattcca
    12061 gctcgttttg gaagtggctg gacaggatat agttggaata gaaacttaat accaaatcca
    12121 gaacagttat tgcaacaact tcatgataga aagttaaaac tctccttaaa tgtccatcct
    12181 gctgatggga tacgggctta tgaagaagct tatcctcaaa tcgcaaaacg gttggggtta
    12241 aatgtagaac tagaagaacc tgctattttt gattttttta atccctcttt tagggaagcc
    12301 tactttaaag atgttcatta tgagctagaa aagcagggag tagatttttg gtggattgac
    12361 tggcaacagg ggacacaagg catgctagat ccactttggc ttttaaacca ttatcactat
    12421 caggatagtt gtaaaaatgc agaaggtggt ttgattttat caagatatgc aggtcctggt
    12481 agtcaccgct accctgttgg tttttcaggg gatactatta tcagttggaa ttccttaaga
    12541 tttcaaccct attttacagc gacagcatct aatatcggtt atagttggtg gagtcatgat
    12601 atcggtggac atatgttggg ggattatgac gaagagctac aaactagatg gctacagttt
    12661 ggtgttttta gtccgattac tcgattacat agttctagaa gtccttttaa tagtaaagaa
    12721 ccttggtttt tttcagaaac aacatctaag attatgaaga aataccttcg tttgagacat
    12781 cagatgattc cttatctata taccatgaat gtaaagacac atgaggaagg tgccccatta
    12841 atcagtccaa tctattattt ctatccagag aatgatgaga gctataatgt tccaaaccaa
    12901 tacttttttg gaacagaact gatggtggct cccattgtag aaaagatgga tttgacattc
    12961 caatctgcaa aagtagatgt atggttccct gaaggtgaat ggtatgactt cttttcagag
    13021 aaaaaataca caggtggtgt gaagttaagt gtttataggg acatctcgac tacgcctgtg
    13081 tttgcaaaaa gtggtgcaat cattcccttg gttggttctg agataggtat gggtgttgat
    13141 ttacctgagg ttgtagattg gtatgtattc ccagggaaac aacattcttt tgaaatgctt
    13201 gaagatcaaa atggtcaaag atataaaaca agattatcaa tcgattggga aatgggaatg
    13261 gtagagttag cattacaagg agattctagt atcgttccaa gcaatagaaa acatagaatt
    13321 cattttaaag gaacgaatgt gtctataatt gaattgccaa ataagaatga tacagctaaa
    13381 tttgaatgga aagataataa aaggacatct ttaaatgacg aagtttttag actactaaag
    13441 acagcttctc ttccatatga attaaaagat agattgttaa atcaattcat caatgcgaaa
    13501 aactctcatg acttaatgaa tatcttgcat catcaggata aggaattgag agggcgtttg
    13561 ttggaaatga tatttactag ccaaaactaa ttgaaataat tagaaagtca tttgtttagt
    13621 gtatatgaag attctgtatg gtattcgtta tcactggtca cattgaaaat gtgaaataag
    13681 cacctacaac atagacttta caacacttca tgtttcatat catggggtgt ttttctattt
    13741 aagtcaaagc caatcaaaaa atctcactat tcatcaggtt aaaggaaacc taatcatagt
    13801 gagattgtga attatagtag agtttgtatc gcctcttcaa tttgttttgg gtctgttttt
    13861 gaagaaaagc gttcaaagac ttgcccatca cgaccaatga gaaacttagc gaaattccat
    13921 tcgattcgtt ttcctagtgg gccagattta tggtctttta accagacata gagagggtct
    13981 gcttccttac cgttgacctt aatcttggca aaacgtggga aggtggtttg aaaatgtagg
    14041 ctacagaagg tgttgatttc ctctgcgctg ccgggtgctt gtcccataaa ctgattgcaa
    14101 gggaaatcca atatttcgaa gccctgatct tgatagcgtt catagagttc ttgaagtctc
    14161 tggtactggg gcgttaaacc acatccagta gcagtgttga caatcaagag aactttgcca
    14221 cgatagctat ccaagggagt tgcttggttg ttttggttca aaacggaaaa atcataaagt
    14281 gaagtcatga gggcctcttt tctttttcta cattgtaaca gtttttactt ttttcgtcga
    14341 gtcggatgga agatattgtt gaagaataag ccctcaatat gatagaaacc gtttacaaat
    14401 aactttaaaa atggtatgat ttaattaaac cggtttactt tgttgctata aatctctaat
    14461 ttgagattta gttcctattg attttacaat atgtttattg gagtgtatac atgcaaacaa
    14521 aaacaaagaa gctcattgtg agtttgtctt cacttgtttt atcaggattt ttattaaacc
    14581 attatatgac agttggagcg gaagaaacga ctacgaatac cattcagcaa agccagaagg
    14641 aagttcagta tcagcaaagg gatacaaaaa atttagttga aaatggtgat tttggtcaga
    14701 cggaggacgg aagcagtccg tggacaggaa gcaaagctca ggggtggtca gcttgggtag
    14761 accagaagaa tagttctgca gatgcctcaa ctcgagtcat tgaggctaag gatggggcta
    14821 tcactatctc aagccctgag aaattaaggg cagcggttca ccgtatggtt cctattgaag
    14881 ctaagaaaaa gtataaactg cgtttcaaga ttaaaacaga taataaagtc gggattgcca
    14941 aggttcgtat cattgaggaa agtggtaagg acaagcgatt gtggaattct gcaacgacgt
    15001 caggaacaaa ggactggcag accattgaag cagactatag cccgacttta gatgttgata
    15061 aaatcaagct ggagttattc tatgaaacag gaactgggac tgtttccttt aaggatattg
    15121 agctggtaga ggtagcagcc cagctttctg aggattctca aacagataaa cagcttgagg
    15181 aaaagattga tttaccaatt ggaaaaaaac atgttttttc tcttgcggac tatacttata
    15241 aggtagaaaa tcctgacgtt gcttcagtca aaaatggaat tttagaacct cttaaggaag
    15301 ggacaaccaa tgtcattgtc agtaaagatg gcaaggaagt gaaaaagatt cctttgaaga
    15361 ttctagcctc tgttaaggat gcatacacag cccgtttgga tgactggaat ggcatcatcg
    15421 ctgggaatca atactatgat tctaaaaatg aacagatggc caaattaaac caggaattgg
    15481 aaggaaaggt agctgatagc ctatctagta tttcaagtca ggcggaccgc acctatttgt
    15541 gggaaaaatt ttcaaattat aaaatgtctg caaatctgac tgccacttat cggaaattgg
    15601 aggagatggc caagcaagtg accaatcctt cttctcgtta ttatcaagat gaaactgtcg
    15661 ttcgaacagt cagggattcc atggaatgga tgcataaaca tgtctacaat cgtgaaaaga
    15721 gcattgttgg gaactggtgg gattatgaaa tcggtacacc tcgtgccatc aacaatacct
    15781 tgtctctgat gaaagaatac ttctctgatg aggaaattaa aaaatataca gatgtgattg
    15841 aaaaatttgt accagatccc gaacatttcc gaaagacgac tgataaccca tttaaggctc
    15901 taggtggaaa cttagttgat atgggaagag taaaagtaat agctggttta ctgcgtaagg
    15961 atgatcaaga aatttcttct accattcgct cgattgagca agtgttcaag ttggtagacc
    16021 aaggtgaagg tttttatcaa gatggatcct atatcgacca caccaatgtt gcctatacgg
    16081 gtgcttatgg gaatgttttg attgatggcc tgtctcaact gttgccagtc attcaaaaga
    16141 ccaagaatcc aatcgataaa gataaaatgc aaaccatgta ccactggatt gataaatcgt
    16201 ttgctccttt gctggtgaat ggagagctga tggatatgag tcgtggacgc tcgatcagtc
    16261 gtgcaaatag cgaggggcac gtggccgcag tagaagtact aagagggatt caccgaatag
    16321 cggatatgtc tgaaggagaa accaaacaac gtttgcagag tcttgtgaag accattgttc
    16381 aatcggatag ttattatgat gtctttaaga atttgaagac ttataaggat atcagtttga
    16441 tgcaatcctt gttaagtgat gcaggagtcg caagtgttcc aagaataagt tacctatctg
    16501 cctttaacaa gatggataaa acagccatgt acaatgcaga gaaagggttt ggatttggct
    16561 tgtcactctt ttccagtcgt accttgaatt acgaacacat gaacaaggaa aataaacgtg
    16621 gttggtatac gagtgatggg atgttctatc tttacaatgg cgatttgagt cactatagcg
    16681 atggctactg gccaacagtt aatccatata agatgcctgg tacaacagag acggatgcta
    16741 agagagcgga tagcgataca ggtaaagttt taccgtctgc tttcgttgga acgagcaaac
    16801 tagatgatgc caatgcgaca gcaaccatgg atttcaccaa ctggaatcaa acattgactg
    16861 ctcataagag ctggtttatg ctaaaggata agattgcctt tttaggaagc aatatccaaa
    16921 acacttcaac agatactgct gcaactacaa tcgaccagag aaaactggaa tcaagtaatc
    16981 catataaagt ctatgtcaat gataaagaag cctcccttac agaacaagaa aaggattatc
    17041 ctgaaaccca aagtgtcttt ttagaatcgt ccgattcgaa aaagaatatt ggttactttt
    17101 tctttaagaa gagttcaatc agcatgagta aggctttgca aaagggagcc tggaaggata
    17161 tcaatgaagg acagtcagac aaggaagttg aaaatgaatt tcttacgatt agtcaggctc
    17221 ataagcaaaa tggagattct tatggctata tgctcattcc taacgtggat cgtgccacct
    17281 tcaatcaaat gataaaagag ttagaaagta gcctcatcga aaataacgaa acccttcagt
    17341 ctgtttatga tgccaaacaa ggagtttggg gcattgtgaa atatgatgat tctgtctcta
    17401 ctatttccaa ccaattccaa gttttgaaac gtggagtcta taccattcga aaagaagggg
    17461 atgaatataa gattgcctac tataatcctg aaacccagga atcagctcca gatcaggaag
    17521 tctttaaaaa gctagagcaa gcagctcagc cacaagtaca gaattcaaaa gaaaaggaaa
    17581 aatctgaaga ggaaaagaac cattcggatc aaaagaatct ccctcagaca ggagaaggtc
    17641 agtcaatctt ggcaagtcta gggttcttgc tacttggggc gttttatcta ttccgtagag
    17701 gaaagaacaa ctaatttgtt catcatctag atgaattaca tgaaactgtg gaaaatagaa
    17761 aaaatccgaa gaaggccaaa tatcttcgga tttttctttt aaaaaaggat aagttgatac
    17821 ttatcctttt tacttcatta tgtgactata gcgttttgaa actagaatag gacatcataa
    17881 ctgctaaaag atttctataa attcatttga ttttcctaat caatttgttc gtatcctatt
    17941 tcactccact atattagtcc gtctcgctcc gtgaggtgcg agacaaaaca accacccgct
    18001 atacgggtgc gcgtcgaagg ttataccaaa aaaactccaa acgcgataca ataaaggtgt
    18061 tcaatccact tgtaaagcga aaggagaaaa aatatggcac aaaaggctca tagtttatct
    18121 cacacaaagt ggaggtgttc tatcacattg tgttcacccc taagtataga cgaaaagtca
    18181 tctataatca atatagaagt agtttaggcg aaatatttca tcgcttgtgt agttataaag
    18241 gtgttgaaat gatcgagggt cacttaatgc cagaccatgt acacatgtta gtcagtattc
    18301 cgtcgaggct aagtgtttca agttttatgg ggtatttaaa aggcaaaagt gcactcatga
    18361 tgtttgacaa acacgccaat ctcaagtaca agtttgggaa tcggcatttc tgggcggaag
    18421 gttattatgt aagtccagta gggcttaatg aagccacaat taagaaatat agtcaagatt
    18481 aaagaaatta tccagtggat gatttcttca cgagtatgaa aatttgagaa cgagtaaagt
    18541 atgatatata gtaaaatgaa ataagaacag gacaaaatcg atcaggacag tcaaatcgat
    18601 ttttaacaat gttttagaag tataggtgta ctatttcagt ttcaatctac tatagcacta
    18661 gataaattga gtgtaaaaga atatgaggat ccctttaggg atagtggtaa gtaataccaa
    18721 agtctcttaa agaggcaagt gacgagtcaa gagcaataag gcttgaacaa cgtgaaagcc
    18781 agcgtcttta ggcgctggct gatgatttgg gcttatagct ctgagataaa ccacccgtta
    18841 gacgggtagt tatgatttta tctgagtgta atatactgtt gggcaatctc gctgatgcgg
    18901 tcaaagttgc cttgggaagc gagtttattg agttcgccac caattccaac ggcatctgca
    18961 ccagcagcga accattgagg gatgttgttt aggccgactc ctccggttac cattacggaa
    19021 acttgtggga tcggtgcctt gactgcagag atatatgctg gactgagagt actacctggg
    19081 aagagtttga tgatttcact accggcttca agtgcagtcg tgatctctgt gagggtaata
    19141 cagcctggaa tgtacggtgt gctgtagaga ttgcacattt tcgcagtttc agcatggaaa
    19201 gatggagaaa caacgtaatt tgctccagct agaatggcat ctctagcagt tacggcatca
    19261 agcacagtac ctgcaccgat acaaacactc tgatcgtcct gatacaagtc tacaagttcc
    19321 ttgatgattt gtcctgcata ctgattggta taggcgattt caatagcttt gataccgccc
    19381 ttgatacaag caatcgaggc ttgtagtcct tcttcctttg tatttcctcg aatgacagcg
    19441 acaattttct gtttttttag ttcaataatc gtatctgatt tggtcatgta attctcctaa
    19501 cgaatgatat cttgtgcatt tgccagtaaa ttttcaatac tagttgcgga agtggagaga
    19561 tggtctcctg gaagagtgca tttgagagtt gcgctcgcaa ctgcaaagtc aatggtagtt
    19621 tttagggagg aatgatggag tagttggtaa agggcaccag atataaaggc atccccgcta
    19681 ccaattcgtt gatagactgc agtttttagt tggacagact cttcaaatcg ttcttccaga
    19741 gcataggctt gatagacatt tttgtcttgc tcatcactag atcggagggt atggaaaata
    19801 gccttgaaac cataggcttc ttttaaaagt cgcatgcgat tttccacctc ttctaggcta
    19861 gcactgtctc ttgggaacat ctctagattt tggtcatcaa tcatgagagg atcaataccg
    19921 aagcaatagt cagtaaaacg tgcaaactta gaaaattcat acttggcttc taggactgaa
    19981 atcatctttg ttctcagatt gagatccatt gaaacgacaa ttcctcggcg cttggcttct
    20041 tccaagagta ggagaaggat cgcacggacc tcttgaccga tagctatggt gattccacta
    20101 aaatgaaaat ggctaatccc ctgaaagaga gaatccatat ctagcatgtt tggccgaatc
    20161 tggctgatac tcgtatgctt acgatcgtag aaaacttcac tttgacgaca accaaagccg
    20221 ttctccaaat agtagaggcc gattcgatcg ccaagccgac agattgaact ggtatcgatt
    20281 tggtgctgtt tcaagaatgt gagaaaacga tctccaatct cgttggcagg gagtgcggta
    20341 aagactttcg ttgagatacc cagggcttgc aaattacaag cgatgttaat ttctgatccg
    20401 ccaaaataag tcgaactggc aacatgatcg ccgatactgg tggcatctaa tggtgagatt
    20461 cgaattaatg gttcgccaaa cagtaatatt ttcgtcatcc attagttcct ttctggttta
    20521 gaatagcttt acttggctaa tacttctttc aaataagcag cgacttcatc atcttggcag
    20581 ttttcaaaga agtattcttg gaagtatttg ccagagatgg tgtctttgag gaagtcttgg
    20641 tcgattgttt taaggatggt catcaaatca ttgtgggtga tttgtttcac ctcgtttaag
    20701 atacgagcgt tgcgttgttc agggattaca cgttctcttg ggtagccacc gcccatttct
    20761 tcagcaaata agcgttcaaa catcaatttg agggtcagtt ctgcccccca gccaaatcct
    20821 ttggcataag ggatagacaa ggcgttacca ccattgattt gagaataaag gtaagcgtca
    20881 gttgggtcca ctgctagacc acagacaaca ccagggaagc tgtttaaagc aagcatagcc
    20941 cctacacccg taccacagcc ggtaacaaca aagtcaactg cctttgtatt taaaaggatg
    21001 gcagccatta gtccgttctg cacataagtt aattgacttt ctccttcttc tccacgcata
    21061 ccatagttaa ataattggta gcctttttta tctgtcgctt cttttagact atcgtaaata
    21121 atgtgattct tgctagcttg actattttca ttgattaatg cgattttcat aagatcttct
    21181 ttctattttt actcaggttg ttttccgatg taggctaaga taccgccatc tacatatagg
    21241 atgtggccat tgacaaaatt gctggcatca ctagcgagaa agacagcagg gcccatcaaa
    21301 tcttcaggat ttccccaacg tgcagcaggt gtttttgcaa tgatgaactg gtcaaatggg
    21361 tggcgagaac catcttcttg caactcacga agaggtgctg tttgaggagt ggcaatataa
    21421 cccggtccaa ttccgttaca ttggatattg gcttcaccgt attcagacgc aatgttgcgg
    21481 gtcaacattt tcaagccccc tttagcagca gcataagcgc taactgtttc acgtcccagt
    21541 tcgctcatca tcgaacaaat attgataatc tttccatgcc ctttctttat catagaagga
    21601 ataactgcct ttgaaacgat aaatggtgcg tttaagtcaa tatcgatgac cttacggaaa
    21661 tcagcggcgc tcatttcgca cattggaact cggcggataa taccagcgtt attaacgagg
    21721 atgtcaatga caccaacctc ttgttcgatt tgcttgacca tggcttggat accgtcctcg
    21781 tctgtcacgt cacagacata tccatgggct tcgatgccaa cttcacgata agcttcaatc
    21841 cctttattga ccagatcttg attgatatcg ttaaagacaa tagtagcgcc ggcttcagcg
    21901 taggatttgg caatagcaaa tccaattcca taagaagcgc cggtgatgag agcaattttt
    21961 ccttttaaag aaaactgctc aattgagaat gatgtatttg tcatttgata cttcctttca
    22021 aatttgttaa gacttctcta taaccaaatt atattctagt aaaccagttt tgtcaataaa
    22081 taaatatttt tatttataaa aagatttatt ttccttaaaa atggaatatt ttgaaatttc
    22141 tgtctattag aatgattaaa aagataatga aagcgctaat atttttttaa aaaaactatt
    22201 gacaagataa aaataaagga ttacaatggg gaatataaag taaaccggta aacctaaaca
    22261 agaaaggaga aaagatgaaa attgtacttg tagggcatgg acattttgct acagggattt
    22321 atagttcttt acaattgatt gcaggcaatc aagaaaatgt ggaggcaatt gactttgtgg
    22381 aaggaatgtc agcagatgaa ctcaagcaaa aaatcttact tgcaatttca aatgaagaag
    22441 aagttttaat cctaagtgat ctcttgggag gatcgccatt caaggtttct tctaccataa
    22501 tgggagaaaa tccagccaaa acaatgaatg ttctttcggg tttgaactta gccatgttaa
    22561 tggaagcagt ctttgctaga atggctcata gctttgatga ggttgttaat aaatcagtag
    22621 tggcggccca gggcggagtc gtaaatggta aagaattgtt ttcaacggat gcagaggaag
    22681 aggaagaaga tttcgaatcg ggtatttaaa gggtaaaaga atgataaaaa aggttacgat
    22741 tgaaaaaata aaatcgcctg agcgcttctt agaagtacca cttctgacga aagaagaagt
    22801 cggccaggca atcgataagg ttattcggca gttagaactc aaccttgact atttcaagga
    22861 agatttcccg acgccagcta cctttgataa tgtctatcca atcatggata acacggaatg
    22921 gaccaatggt ttctggacag gagaactgtg gttggcttat gaatacagtc aacaggatgc
    22981 atttaaaaac atcgctcata aaaatgttct ttctttcctg gatcgtgtca ataagagagt
    23041 agaattggat caccatgatc tcggcttctt gtacacaccg tcttgtatgg ctgaatataa
    23101 gataaatgga gatggagagg ctagagaagc aaccttgaaa gctgcagata agttgattga
    23161 acgctatcaa gaaaaaggtg gttttattca agcttgggga gacttgggca agaaagagca
    23221 ttaccgtttg attatcgact gcttgctcaa tatccaactc ttattctttg cttatcaaga
    23281 aacaggcgat caaaaatact acgatattgc agaaagtcat ttctatgctt cagctaataa
    23341 tgtaatccgt gatgacgctt cgtccttcca caccttctat tttgatcctg agacaggtca
    23401 accctttaaa ggtgtaacga gacaagggta tagtgatgat tcatgctggg cacgtggtca
    23461 atcatgggga gtctatggta ttcctttgac ttatcgtcac ttaaaagacg agtcctgctt
    23521 tgacttgttt aagggtgtga ccaattattt cttgaatcgt ctgccaaaag atcatgtgtc
    23581 ctattgggat ttgattttta atgatggtag tgctcaatca cgagattctt cagcaacagc
    23641 tatcgccgtc tgtgggattc atgaaatgct aaaacatctc ccagaggtgg atgctgacaa
    23701 agatatttat aaacatgcta tgcatgccat gcttcgttcc ttgatcgaac attatgcaaa
    23761 tgatcaattt acccctggtg ggacaagtct cctccacggt gtgtactcat ggcattcagg
    23821 taaaggagtg gatgaaggca atatctgggg tgactactat tacctagaag cccttatccg
    23881 tttctacaaa gactggaacc tatattggta ggaggagaaa tatgacaatg ccaaatatta
    23941 ttatgacccg tatcgatgaa cggttgattc atgggcaagg acaactttgg gtaaaatacc
    24001 taggttgtaa tacggtcatt gttgccaatg acgaagtaag cacggacaag atgcaacaaa
    24061 ctctgatgaa aacagttgtg ccagactcag ttgccatgcg tttcttccct ttgcaaaagg
    24121 tgattgatat cattcacaag gctaatcctg ctcaaacgat ctttatcgtt gtaaaggatg
    24181 tgaaggatgc tttaaccttg gtagaaggtg gtgtccctat caaagaaatc aatattggga
    24241 acattcacaa tgctactggt aaagagcaag tgacacgctc catcttcctg ggtgaagagg
    24301 acaaggcggc cctcaaggaa ttgagccaaa ctcatcaagt aacatttaat acgaaaacaa
    24361 ctccaacagg aaatgatgga gctgttcaag tcaacattat ggactatatt taaaagagga
    24421 gatcgttatg tcgattaatg tatttcaagc gattttaatt ggattatgga cagctttctg
    24481 ttttagtgga atgctgttag gaatttacac caatagatgt attgttctgt catttggtgt
    24541 cggaattatt ctaggtgatc tgcctactgc tcttgcaatg ggagctattg gtgaattggc
    24601 ttatatggga ttcggtgttg gtgctggagg tactgttcca ccaaacccaa tcggacctgg
    24661 tatctttggt accttgatgg ctatcactag tgctggtaaa gtcagtccag aagcggctct
    24721 tgccctctct actccgattg ctgtggcgat tcaattctta caaactttcg cctacactgt
    24781 acgtgctggt gcgcctgaaa cagcgatgaa gcacttgaaa aaccataatt tgaagaaatt
    24841 caagttcact ctaaatgcaa caatttggtt gtttgccttt attggattta ccttgggttg
    24901 cttgggtgcc ctttcaatgg ataccttgtt gaaactcgta gactacattc caccggtatt
    24961 gcttacaggt ttgacagttg ctggtaaaat gctcccagct atcggttttg cgatgatctt
    25021 gtcagtgatg gctaagaaag agttgattcc ctttgtcttg ttgggatatg tttgtgcagc
    25081 ttatctaaac atcccaacaa ttggtattgc aattgtaggt actatctttg ctttgattga
    25141 attttataac aagccaaaaa cagcggatca tgtggtagag gaggaagcac acgatgactg
    25201 gatctaacaa attaacaaaa cgtgattatc ttaaaacgtc tttgcgggca ttcttttgtc
    25261 aaaatggatt taactatagt aactatcaag ggttgggata tgccaatgtg atgtatcctg
    25321 ctttgaaaaa acactatgga gaggatcagg aaggtttcta ccaagccttg gaagaaaact
    25381 gtgaattcta taataccaac ccacacttcc tgccttttat taccagcttg catcttgtaa
    25441 tgttggaaaa tggccgtccg gcaaaagaaa cacgtagcat caagatggcc ttgatgggac
    25501 cattggcagg tattggggat tctctttctc aattctgttt agctccattg ttctcaacca
    25561 tcgcagcttc gtttgctcaa gaaggcttgg ttgtcggtcc aatcttgttc ttccttgcga
    25621 tgaatacgat tttaacagcg attaaattgt caactggtct gtatggatac aaactaggaa
    25681 caactgtgat tgataaacta agcgaacaga tggcaacgat ttctcgtatt gccaatatta
    25741 tcggtgtaac cgtaattgct ggtttggcag cgacatctgt taagattatg gtgccgatta
    25801 cctttgctgc aggggaagtt aaagcagacg ctaaacaaag tatcgtaagt attcagggaa
    25861 tgcttgataa ggttgctcca gctcttctac cagccctatt tacactttta gtttactact
    25921 tgatcaaaga aaagaaatgg acaacatata aactcgttat tttaacagtt atcatcggaa
    25981 ttatcggaag ctggcttaag attatagctt aatggataca acattgtttt atggaatagt
    26041 gattgtcttg gcagtgagtc cccttttact gtcaagcttt cattctattc gtcagcaaaa
    26101 gttgcttcgc aaacagatgg agcaacgaca agagtattta gcttctttaa catctggtga
    26161 tgaagtgttg ttattgtctg gaattcatgg aaaaatcatt tctatccaag atgacttggt
    26221 ctccttgcag attgcaaaag gtgtggtcat ctatgtagaa aaggaaagtg taatgggaaa
    26281 gacaaaagaa ctgcttttta agtagttctt tttctggtaa tagataatga aagaagaaag
    26341 aagacaattt tttgaaagag tcgatggaaa ccaatgtcgt gattatatct tgtcccactg
    26401 ttcaaaagac tatgagaaag tcaagagttc ccttgaacgc ttgatagaca atcgttttat
    26461 gtttgatagt ccttgggata tggagccttg ttcaaaaatc catcaaatcc agccgatggt
    26521 atgggatcaa gtatttgaag atgatccaga atggtcttat atgctcaatc gacaagaata
    26581 tctcttgcag tttatgatag ggtatctggt agaaggagat aagggctata tccaaaagtg
    26641 caagttcttt ctatttgatt ggattgagca ggtgagagaa ttttctcctc aatccttgat
    26701 gactagaacc ttggatacgg gtattcgttc ctttacttgg ttgaaactac tcttgctcct
    26761 cttgaaattt gacttgctag aggagaaaga actagagaaa attttggtca gtctagaaaa
    26821 gcagattgac tttatgaaaa gccactatcg cgccaagtac acccttagta actgggggat
    26881 tttacaaaca attccgatgc ttgctatcta ttatttcttt tcagataaga tggacctaga
    26941 agaagcttac cattttgctt cagaggagtt gaaacagcaa attgagacac agattttagg
    27001 agatggaagc cagtttgaac agtcgattct ctatcatgta gaggtttata aagccttgct
    27061 ggatttgtgt ctcttgcttc cagacttgca agatagttac cgagagttgc tggaaaagat
    27121 ggcgacctat attcaaatga tgacaggctt agatggacga actttggctt ttggtgatag
    27181 cgattctaca gaaacgacag aaattttgag cctgtctgct gtggttttga acaaggaaga
    27241 ccttcttaac ggtctggatg ttaaagtcga tttgcttagc ctcttgttcc tggggcgaga
    27301 aaaggtcaag cgactgcagg aatttgaaag gagagcttgg cagcctaagt ccatgatctt
    27361 tgaagactct ggacatgtct gcattaagga tgaacatcgt tatctatttt tcaaaaatgg
    27421 tccgctagga agtgcccata gccatagtga cgagaatagt ttttgcttac agtatcaagg
    27481 ccaacctatt ttcatagatg ctgggcgtta ttcttatcgg gagatatatg aacgttatct
    27541 cttaaagagt gcttggagtc attcgacctg cattgtagat gggaaagctc cggaaagaat
    27601 cacgggatcc tgggaatatg aatactatcc tcactccctg ttttgtcacc ataaagaaag
    27661 ggagggagtg cattatattg agggggctta ttggtcagca gaacctgatt tgccttatct
    27721 tcacaagaga aaaatcctca tgttggtaga ggatgtctgg ctcttggtag atgacatcag
    27781 gtgtcaaggt cagcatgagg cgttgactca gtttatcctt gacaaggatg tgacctatca
    27841 agatgggaaa atcaatcagt tgagactatg gagtgaagtt gattttgatt tggaagatac
    27901 catcatttct cctaaataca atgagcttga aagaagtagc aaactcacca agcgccaatt
    27961 ctttgagaat cagatgctgg attataccat cattgcgcat gagagttttg aaattatccg
    28021 tcattctgtc taccagacag atgatcgtga agtggaaaat gctctggctt ttgaagtgaa
    28081 aaatgacgaa acagacaagc tgattctgtt attaagcgag gatattggtg taggtgaaaa
    28141 attgtgcctc gttgacggaa caaaaatgcg tggaaaatgt ctagtatatg ataaaataaa
    28201 tgagagaatg attcgcttgc agtgctagaa ataggcattt tgaatagtga atatgttata
    28261 ataagtatta gtaggaggtg ttttagattg gagaagaaac tgaccataaa agacattgcg
    28321 gaaatggctc agacctcgaa aacaaccgtg tcattttacc taaacgggaa atatgaaaaa
    28381 atgtcccaag agacacgtga aaagattgaa aaagttattc atgaaacaaa ttacaaaccg
    28441 agcattgttg cgcgtagctt aaactccaaa cgaacaaaat taatcggtgt tttgatcggt
    28501 gatattacca acagtttctc aaaccaaatt gttaagggaa ttgaggatat cgccagccag
    28561 aatggctacc aggtaatgat aggaaatagt aattacagcc aagagagtga ggaccggtat
    28621 attgaaagca tgcttctctt gggagtagac ggctttatta ttcagccgac ctctaatttc
    28681 cgaaaatatt ctcgtatcat cgatgagaaa aagaagaaaa tggtcttttt tgatagtcag
    28741 ctctatgaac accggactag ctgggttaaa accaataact atgatgccgt ttatgacatg
    28801 acccagtcct gtatcgaaaa aggttatgaa cattttctct tgattacagc ggatacgagt
    28861 cgtttgagta ctcggattga gcgggcaagt ggttttgtgg atgctttaac agatgctaat
    28921 atgcgtcacg ccagtctaac cattgaagat aagcatacga atttggaaca aattaaggaa
    28981 tttttacaaa aagaaatcga tcccgatgaa aaaactctgg tatttatccc taactgttgg
    29041 gccctacctc tagtctttac cgttatcaaa gagttgaatt ataacttgcc acaagttggg
    29101 ttgattggtt ttgacaatac ggagtggact tgcttttctt ctccaagtgt ttcgacgctg
    29161 gttcagccct cctttgagga aggacaacag gctacaaaga ttttgattga tcagattgaa
    29221 ggccgcaatc aagaagaaag gcaacaagtc ttggattgta gtgtgaattg gaaagagtcg
    29281 actttctaaa atgaaggaaa atgacttgca atttctgtta agaaataaaa taatcccacc
    29341 tagaacaagc taggtgggat tatttgccta tgaaatgaga aattatggga gcaagctcct
    29401 aaatcaactg tttttgatct acttctttaa ctacttgata aaagttatag aagtaggcca
    29461 aacttgaaat gatggttacg actaggaata ttgaaaattt ccattggaca gggttggtta
    29521 aaagttgtgg aaaggatatg aggagaaaga agagggctgc gttgaggaca ggtatccgtt
    29581 ttgattgtat tttctcaagt cctttattga gcgcaggaag aaagaggagt aggagtagta
    29641 aaactgtatg agaaacagct cctgaaataa gggcgaagaa aaggaaaata ctgataaaaa
    29701 catgaatgat cagtagtcta gctagtgatt tcataaggca cctcctaatc ctggtctttt
    29761 ttagctcttg caatacgaag tgagtcgaca atatgtatca tcactccgaa aaagaaagct
    29821 cccagtatag ttttaaaaat atgttttgta tttagaagag aactgataaa atttggattt
    29881 tcacttgtta gggtatcaat gagtggaatt ataaaaaata tcactgttcc ataaatcgaa
    29941 cctgctttca gaccaggata acgtaactgt ttcttttctt ttttcatgag tttgctcctt
    30001 ttttaaaaat cttccttcca aaagagactg ttgaggtcag tttggaggct gcgagcgaga
    30061 ttgagacaga gttccagagt tggattgtac ttgtcgtttt caatcatatt gatggtctgt
    30121 ctcgagacac cgatatcctt ggcgagctcg agctgggaaa tgcccagttc cttgcgaaat
    30181 tctttcacac gattcatctg gtctcctttc tgatttgtgt cgtatatatt tgactttatt
    30241 atagtctttt aaacataaag tgtcaagtat ttttgacata ttttttgaag aaatagtagt
    30301 ctccttgtcc tatttgtctg acaagtgcaa gctggtcgga tttgtggtaa aatagataag
    30361 atatgacaaa agaatttcat catgtaacgg tcttactcca cgaaacgatt gatatgcttg
    30421 acgtaaaacc tgacggtatc tacgttgatg cgactttggg tggagcaggc catagcgagt
    30481 atttattaag taaattaagt gaaaaaggcc atctctatgc ctttgaccag gatcagaatg
    30541 ccattgacaa tgcgcaaaaa cgcttggcac cttacattga gaagggaatg gtgaccttta
    30601 tcaaggacaa cttccgtcat ttacaggcac gtttgcgcga agctggtgtt caggaaattg
    30661 atggaatttg ttatgacttg ggagtgtcta gtcctcaatt agaccagcgt gagcgtggtt
    30721 tttcttataa aaaggatgcg ccactggaca tgcggatgaa tcaggatgct agtctgacag
    30781 cctatgaagt ggtgaacaat tatgactatc atgacttggt tcgtattttc ttcaagtatg
    30841 gagaggacaa attctctaaa cagattgcgc gtaagattga gcaagcgcgt gaagtgaagc
    30901 cgattgagac aacgactgag ttagcagaga ttatcaagtt ggtcaaacct gccaaggaac
    30961 tcaagaagaa gggtcatcct gctaagcaga ttttccaggc tattcgaatt gaagtcaatg
    31021 atgaactggg agcggcagat gagtccatcc agcaggctat ggatatgttg gctctggatg
    31081 gtagaatttc agtgattacc tttcattcct tagaagaccg cttgaccaag caattgttca
    31141 aggaagcttc aacagttgaa gttccaaaag gcttgccttt catcccagat gatctcaagc
    31201 ccaagatgga attggtgtcc cgtaagccaa tcttgccaag tgcggaagag ttagaagcca
    31261 ataaccgctc gcactcagcc aagttgcgcg tggtcagaaa aattcacaag taagagggaa
    31321 aaagatggca gaaaaaatgg aaaaaacagg tcaaatacta cagatgcaac ttaaacggtt
    31381 ttcgcgtgtg gaaaaagctt tttacttttc cattgctgta accactctta ttgtagccat
    31441 tagtattatt tttatgcaga ccaagctctt gcaagtgcag aatgatttga caaaaatcaa
    31501 tgcgcagata gaggaaaaga agaccgaatt ggacgatgcc aagcaagagg tcaatgaact
    31561 attacgtgca gaacgtttga aagaaattgc caattcacac gatttgcaat taaacaatga
    31621 aaatattaga atagcggagt aagatatgaa gtggacaaaa agagtaatcc gttatgcgac
    31681 caaaaatcgg aaatcgccgg ctgaaaacag acgcagagtt ggaaaaagtc tgagtttatt
    31741 atctgtcttt gtttttgcca tttttttagt caattttgcg gtcattattg ggacaggcac
    31801 tcgctttgga acagatttag cgaaggaagc taagaaggtt catcaaacca cccgtacagt
    31861 tcctgccaaa cgtgggacta tttatgaccg aaatggagtc ccgattgctg aggatgcaac
    31921 ctcctataat gtctatgcgg tcattgatga gaactataag tcagcaacgg gtaagattct
    31981 ttacgtagaa aaaacacaat ttaacaaggt tgcagaggtc tttcataagt atctggacat
    32041 ggaagaatcc tatgtaagag agcaactctc gcaacctaat ctcaagcaag tttcctttgg
    32101 agcaaaggga aatgggatta cctatgccaa tatgatgtct atcaaaaaag aattggaagc
    32161 tgcagaggtc aaggggattg attttacaac cagtcccaat cgtagttacc caaacggaca
    32221 atttgcttct agttttatcg gcctagctca actccatgaa aatgaagatg gaagcaagag
    32281 cttgctggga acctctggaa tggagagttc cttgaacagt attcttgcag ggacagacgg
    32341 cattattacc tatgaaaagg atcgtctggg taatattgta cccggaacag aacaagtttc
    32401 ccaacgaacg atggacggta aggatgttta tacaaccatt tccagccccc tccagtcctt
    32461 tatggaaacc cagatggatg cttttcaaga gaaggtaaaa ggaaagtaca tgacagcgac
    32521 tttggtcagt gctaaaacag gggaaattct ggcaacaacg caacgaccga cctttgatgc
    32581 agatacaaaa gaaggcatta cagaggactt tgtttggcgt gatatccttt accaaagtaa
    32641 ctatgagcca ggttccacta tgaaagtgat gatgttggct gctgctattg ataataatac
    32701 ctttccagga ggagaagtct ttaatagtag tgagttaaaa attgcagatg ccacgattcg
    32761 agattgggac gttaatgaag gattgactgg tggcagaatg atgacttttt ctcaaggttt
    32821 tgcacactca agtaacgttg ggatgaccct ccttgagcaa aagatgggag atgctacctg
    32881 gcttgattat cttaatcgtt ttaaatttgg tgttccgacc cgtttcggtt tgacggatga
    32941 gtatgctggt cagcttcctg cggataatat tgtcaacatt gcgcaaagct catttggaca
    33001 agggatttca gtgacccaga cgcaaatgat tcgtgccttt acagctattg ctaatgacgg
    33061 tgtcatgctg gagcctaaat ttattagtgc catttatgat ccaaatgatc aaactgctcg
    33121 gaaatctcaa aaagaaattg tgggaaatcc tgtttctaaa gatgcagcta gtctaactcg
    33181 gactaacatg gttttggtag ggacggatcc ggtttatgga accatgtata accacagcac
    33241 aggcaagcca actgtaactg ttcctgggca aaatgtagcc ctcaagtctg gtacggctca
    33301 gattgctgac gagaaaaatg gtggttatct agtcggggta accgactata ttttctcggc
    33361 tgtatcgatg agtccggctg aaaatcctga ttttatcttg tatgtgacgg tccaacaacc
    33421 tgaacattat tcaggtattc agttgggaga atttgccaat cctatcttgg agcgggcttc
    33481 agctatgaaa gactctctca atcttcaaac aacagctaaa gctttggagc aagtaagtca
    33541 acaaagtcct tatcctatgc ctagtgtcaa ggatatttca cctggtgatt tagcagaaga
    33601 attgcgtcgc aatcttgtac aacccatcgt tgtgggaaca ggaacgaaga ttaaaaacag
    33661 ttctgctgaa gaagggaaga atcttgcccc gaaccagcaa gtccttatct tatctgataa
    33721 agtagaggaa gttccagata tgtatggttg gacaaaggag actgctgaga cccttgctaa
    33781 gtggctcaat atagaacttg aatttcaagg ttcgggctct actgtgcaga agcaagatgt
    33841 tcgtgctaac acagctatca aggacattaa aaaaattaca ttaactttag gagactaata
    33901 tgtttatttc cctcagtgct ggaattgtga catttttact aactttagta ggaattccgg
    33961 cctttatcca attttataga aaggcgcaaa ttacaggcca gcagatgcat gaggatgtca
    34021 aacagcatca ggcaaaagct gggactccta caatgggagg tttggttttc ttgattactt
    34081 ctgttttggt tgctttcttt ttcgccctat ttagtagcca attcagcaat aatatgggaa
    34141 tgattttgtt catcttggtc ttgtatggct tggtcggatt tttagatgac tttctcaagg
    34201 tctttcgtaa aatcaatgag gggcttaatc ctaagcaaaa attagctctt cagcttctag
    34261 gtggagttat cttctatctt ttctatgagc gcggtggcga tatcctgtct gtctttggtt
    34321 atccagttca tttgggattt ttctatattt tcttcgctct tttctggcta gtcggttttt
    34381 caaacgcagt aaacttgaca gacggtgttg acggtttagc tagtatttcc gttgtgatta
    34441 gtttgtctgc ctatggagtt attgcctatg tgcaaggtca gatggatatt cttctagtga
    34501 ttcttgccat gattggtggt ttgctcggtt tcttcatctt taaccataag cctgccaagg
    34561 tctttatggg tgatgtggga agtttggccc taggtgggat gctggcagct atctctatgg
    34621 ctctccacca agaatggact ctcttgatta tcggaattgt atatgttttt gaaacaactt
    34681 ctgttatgat gcaagtcagt tatttcaaac tgacaggtgg taaacgtatt ttccgtatga
    34741 cgcctgtaca tcaccatttt gagcttgggg gattgtctgg taaaggaaat ccttggagcg
    34801 agtggaaggt tgacttcttc ttttggggag tgggacttct agcaagtctc ctgaccctag
    34861 caattttgta tttaatgtaa gaatggcacc ctgatgtttc agggtgtttt tgtatttaaa
    34921 tacacaatga aaatcaaaga acaaactaga aagctaactt taggctgctc aaaatatagt
    34981 agattgaaac tagaatagta cacatctgct tctaaaatat tgttagaaat cgatttgact
    35041 gtcctgatcg atttgtcctg ttcttatttc attttaatat atagttttga gatggttgat
    35101 agaactgacg aagtcagctc aaaacatggt tttgaggttg tagataagga gaagctgatg
    35161 tggtttgaag aggttttcga agagtgtaaa aaaatattgg tcagttaaag tcaaaactct
    35221 tgaccttttc tgaccaatgt agtatattat gaacgtaagg taaatgaaag ccttactaga
    35281 acacttattt aaagtaaaat agaaagaggt gggtctatgt ttcatctaga aattttcaga
    35341 agtaaagata gtctactcct gcttgaaaaa gaaaaaccag aaatagtacg tagggtagcg
    35401 gtttagctag tctaaatttt ttaaaacaat ggtcaaagat agtcaatatc agtagtcata
    35461 actaagtaaa caaaaagagg taaagaatat gaataacaac tttaataatt ttaataacat
    35521 ggatgattta tttaaccaat tgatgggtgg tatgcgagga tatagttctg aaaatcgccg
    35581 ttacttaatt aatggacgcg aagtcacacc tgaggaattt gctcactatc gtgcgactgg
    35641 tcaattacca ggaaatgcag aaactgatgt gcaaatgcca caacaggcat caggtatgaa
    35701 acaagacggt gtccttgcaa aactaggtcg aaacttggca gcagaagcgc gtgagggcaa
    35761 gttggatcct gttatcggac gaaacaagga aattcaagaa acatctgaaa tcctctcacg
    35821 tcgcaccaag aacaatcctg ttttggtcgg agatgcaggt gttggtaaga cagcagttgt
    35881 cgaaggtcta gcgcaagcca ttgtgaacgg agatgttcct gctgctatca agaacaagga
    35941 aattatttct attgatatct caggtcttga ggctggtact caataccgtg gtagctttga
    36001 agaaaatgtc caaaacttag tcaatgaagt gaaagaagca gggaatatta tcctcttctt
    36061 tgatgaaatt caccaaattc ttggtgctgg tagcactggt ggagacagtg gttctaaagg
    36121 acttgcggat attctcaagc cagctctctc tcgtggagaa ttgacagtga ttggggcaac
    36181 aactcaagac gaataccgta acaccatctt gaagaatgct gctcttgctc gtcgtttcaa
    36241 cgaagtgaag gtcaatgctc cttcagcaga gaatactttt aaaattcttc aaggcattcg
    36301 tgacctctat caacaacacc acaatgtcat cttgccagac gaagtcttga aagcagcggt
    36361 ggattattct gttcaataca ttcctcaacg tagcttgcca gataaggcta ttgaccttgt
    36421 cgatgtaacg gctgctcact tggcggctca acatccagta acagatgtgc atgctgttga
    36481 acgagaaatc gaaacggaaa aagacaagca agaaaaagca gttgaagcag aagattttga
    36541 agcagctcta aactataaaa cacgcattgc agaattggaa aagaaaatcg aaaaccacac
    36601 agaagatatg aaagtgactg caagtgtcaa cgatgtggct gaatctgtgg aacgaatgac
    36661 aggtatccca gtatcgcaaa tgggagcttc agatatcgaa cgtttgaaag atatggctca
    36721 tcgcttgcaa gacaaggtga ttggtcaaga taaggccgta gaagctgtag ctcgtgctat
    36781 ccgtcgtaac cgtgctggtt ttgatgaagg aaatcgccca atcggcagct tcctctttgt
    36841 agggtctact ggggttggta agacggagct tgctaagcaa ttggcactcg atatgtttgg
    36901 aacccaggat gcgattatcc gtttggatat gtctgaatac agtgaccgca cagctgtttc
    36961 taagctaatt ggtacaacag caggctatgt gggttatgat gacaatagca ataccttaac
    37021 agaacgtgtt cgtcgcaatc catactctat cattctcttg gatgaaattg aaaaggctga
    37081 ccctcaagtt attacccttc tcctccaagt tctagatgat ggtcgtttga cagatggtca
    37141 aggaaataca gtaaacttca agaacactgt cattattgcg acctcaaatg ctggatttgg
    37201 ctatgaagcc aacttgacag aagatgcgga taaaccagaa ttgatggacc gtttgaaacc
    37261 cttcttccgt ccagaattcc tcaaccgctt taatgcagtc atcgaattct cacacttgac
    37321 taaggaagac ctttctaaga ttgtagattt gatgttggct gaagttaacc aaaccttggc
    37381 taagaaagac attgacttgg tagtcagtca agcggctaaa gattatatca cagaagaagg
    37441 ttacgacgaa gtcatgggag ttcgtcctct ccgtcgagtg gttgaacaag aaattcgtga
    37501 taaggtgaca gacttccact tggatcattt agatgctaaa catctggaag cagatatgga
    37561 agatggtggt ttggtcattc gtgaaaaatc ctaatactct tcgaaaatca aattcaaacc
    37621 acgtcaacgt cgccttgccg tactcaagta cagcctgcgg ctagtttcct agtttgctct
    37681 ttgattttca ttgagtataa gatagaattt tgaggataaa aaagaaggag ccagctgaaa
    37741 aaaactggtt ccttttgagg tacgacgggc atgtcgtgca gtagaggtgt actattc
//
LOCUS       DAVKLW010000015        35873 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKLW010000015 DAVKLW010000000
VERSION     DAVKLW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.015"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            821..1042
                     /locus_tag="VW324_000738"
     CDS             821..1042
                     /locus_tag="VW324_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9726412.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1559..1812
                     /locus_tag="VW324_000739"
                     /pseudo
     CDS             1559..1812
                     /locus_tag="VW324_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1940..3169
                     /locus_tag="VW324_000740"
     CDS             1940..3169
                     /locus_tag="VW324_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9726413.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3189..5885
                     /gene="ppc"
                     /locus_tag="VW324_000741"
     CDS             3189..5885
                     /gene="ppc"
                     /locus_tag="VW324_000741"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9726414.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5968..6185
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6298..7302
                     /locus_tag="VW324_000742"
     CDS             6298..7302
                     /locus_tag="VW324_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9726415.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7371..8237
                     /locus_tag="VW324_000743"
     CDS             7371..8237
                     /locus_tag="VW324_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726416.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8234..8992
                     /locus_tag="VW324_000744"
     CDS             8234..8992
                     /locus_tag="VW324_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726417.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9082..10842
                     /gene="dnaG"
                     /locus_tag="VW324_000745"
     CDS             9082..10842
                     /gene="dnaG"
                     /locus_tag="VW324_000745"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9726418.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10845..11954
                     /gene="rpoD"
                     /locus_tag="VW324_000746"
     CDS             10845..11954
                     /gene="rpoD"
                     /locus_tag="VW324_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9726419.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11968..12297
                     /locus_tag="VW324_000747"
     CDS             11968..12297
                     /locus_tag="VW324_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9726420.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12331..13455
                     /gene="cpoA"
                     /locus_tag="VW324_000748"
     CDS             12331..13455
                     /gene="cpoA"
                     /locus_tag="VW324_000748"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9726421.1"
                     /translation="MRKFPLFSSSLSFLLLILFKENDIIVVMEKKKLRINMLSSSEKV
                     AGQGVSGAYRELVRLLHRAAKDQLIVTENLPIEADVTHFHTIDFPYYLSTFQKKRSGR
                     KIGYVHFLPATLEGSLKIPFFLKGIVKRYVFSFYNRMEHLVVVNPMFIEDLVAAGIPR
                     EKVTYIPNFVNKEKWHPLPQEEVVRLRTDLGLSDNQFIVVGAGQVQKRKGIDDFIRLA
                     EELPQITFIWAGGFSFGGMTDGYEHYKKIMENPPKNLIFPGIVSPERMRELYALADLF
                     LLPSYNELFPMTILEAASCEAPIMLRDLDLYKVILEGNYRATAGREEMKEAILEYQAN
                     PAVLKDLKEKAKNISREYSEEHLLQIWLDFYEKQAALGRK"
     gene            13471..14796
                     /locus_tag="VW324_000749"
     CDS             13471..14796
                     /locus_tag="VW324_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9726422.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14827..15003
                     /locus_tag="VW324_000750"
     CDS             14827..15003
                     /locus_tag="VW324_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9726423.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15062..15196
                     /locus_tag="VW324_000751"
     CDS             15062..15196
                     /locus_tag="VW324_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9726424.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15260..16570
                     /gene="obgE"
                     /locus_tag="VW324_000752"
     CDS             15260..16570
                     /gene="obgE"
                     /locus_tag="VW324_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9726425.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGGDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16580..16738
                     /locus_tag="VW324_000753"
     CDS             16580..16738
                     /locus_tag="VW324_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726426.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17825..19084
                     /locus_tag="VW324_000754"
     CDS             17825..19084
                     /locus_tag="VW324_000754"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9726427.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            19100..19651
                     /locus_tag="VW324_000755"
     CDS             19100..19651
                     /locus_tag="VW324_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726428.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19644..20921
                     /locus_tag="VW324_000756"
     CDS             19644..20921
                     /locus_tag="VW324_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9726429.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20937..21797
                     /locus_tag="VW324_000757"
     CDS             20937..21797
                     /locus_tag="VW324_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9726430.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            22178..22963
                     /locus_tag="VW324_000758"
                     /pseudo
     CDS             22178..22963
                     /locus_tag="VW324_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            23154..25445
                     /gene="pcrA"
                     /locus_tag="VW324_000759"
     CDS             23154..25445
                     /gene="pcrA"
                     /locus_tag="VW324_000759"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9726431.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            25488..26168
                     /gene="radC"
                     /locus_tag="VW324_000760"
     CDS             25488..26168
                     /gene="radC"
                     /locus_tag="VW324_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9726432.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDYVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(26165..26854)
                     /locus_tag="VW324_000761"
     CDS             complement(26165..26854)
                     /locus_tag="VW324_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9726433.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYATKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(26872..27513)
                     /locus_tag="VW324_000762"
     CDS             complement(26872..27513)
                     /locus_tag="VW324_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9726434.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(27596..27811)
                     /locus_tag="VW324_000763"
     CDS             complement(27596..27811)
                     /locus_tag="VW324_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9726435.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(27819..28166)
                     /locus_tag="VW324_000764"
     CDS             complement(27819..28166)
                     /locus_tag="VW324_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9726436.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(28171..29286)
                     /locus_tag="VW324_000765"
     CDS             complement(28171..29286)
                     /locus_tag="VW324_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9726437.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPKNQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(29296..30255)
                     /locus_tag="VW324_000766"
     CDS             complement(29296..30255)
                     /locus_tag="VW324_000766"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9726438.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(30368..30937)
                     /locus_tag="VW324_000767"
     CDS             complement(30368..30937)
                     /locus_tag="VW324_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9726439.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            31065..31736
                     /locus_tag="VW324_000768"
     CDS             31065..31736
                     /locus_tag="VW324_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9726440.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            31720..32538
                     /locus_tag="VW324_000769"
     CDS             31720..32538
                     /locus_tag="VW324_000769"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9726441.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            32535..33431
                     /locus_tag="VW324_000770"
     CDS             32535..33431
                     /locus_tag="VW324_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9726442.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            33475..34449
                     /gene="pta"
                     /locus_tag="VW324_000771"
     CDS             33475..34449
                     /gene="pta"
                     /locus_tag="VW324_000771"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9726443.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(34710..35873)
                     /locus_tag="VW324_000772"
                     /pseudo
     CDS             complement(34710..35873)
                     /locus_tag="VW324_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10831 a         6381 c         7871 g        10790 t
ORIGIN      
        1 tttcttttat ttttacctat agctatcttt atgcaaattt gctgtataag atttgattcc
       61 attttagcaa aggccttacc taatattttg ctgatactta ttgttttaat cacgtttcag
      121 agtgattgga atatatttat acctgcaact tattattata cagaaattca gtcaactaca
      181 aacttaggta ttaaattatt agtttgtatc tggattatgg gatttgaatt tttccttctt
      241 ccaaaactaa taaaattgaa agaacaaaat ctagtttaaa tgttgctcga aaattaaata
      301 attgaatacg attaacaatg aaacaataca acgtgagtca tgacttcaaa gtaattgatt
      361 ttatgattct ggtttcttac gcttaattaa gcaagttgta ttcactttat caaattgtta
      421 ttactctaaa aagcgtctaa cagctataga gtgtctgttt gcttctcttt acttttattt
      481 taaataattc attaaccttc cgtaagtgcc caataataag gtgtgttgaa gaagtctgag
      541 ataaaatagt tctcagtcac aaaaaagcta tagattccca ttggtgaaca gctccctgtc
      601 aagtagacag taaaataata taaaatctta agcgacctga ttcctgaatt ccagtggagt
      661 caggttgttt tttaaccgtc tttcttaata ttgtcttacc tataatagat atgcttatga
      721 gatatgttta ataaattaat tttagttcaa aatgtccagt taggacactt tgaatgattt
      781 cttagtttta tatattatac ttttctcagg agaaagctag atgtacagac gtttgagaga
      841 tttgagggaa gatcacgatt tgacccaaaa acaaatagct aaaatacttt cgtttacaaa
      901 ctcagcttat gctaaaattg aacggggtga gcatacgtta acagcagatg tattggtaac
      961 tctctcaaac ttttacgatg tcagtacaga ctacctattg gaattgacag attttcctga
     1021 taaaattcgc tttagaaaat aatctcctca atttcataga gtgtgaaaat gaggagattt
     1081 tttatttgtt ctttgaaaat taaatagtcc aaaatggtac tttcctcatt tgtggagcaa
     1141 atttgaatgg ctcgccatga taagagcgat tttaaaacca tcaataaaat agagcgatac
     1201 tttatatgcc atgatacaaa tgatatacaa tgatacttct gaccgttcag gctgccatcg
     1261 taaaagagca gcaagtgaaa ttcttatgat gacttcatca gtcatgccac gttaaatgtg
     1321 tgagtttgtt agagaaacgc aattaatcct caaaaggttc cccgaacctt ttgagttcca
     1381 cagacgcatc acgtggagtg tgtaagcttg ttgctaaaag cgtaaaaacc ttggaacgaa
     1441 aggaataata gacttcctgc gaaacaaaaa tacaatacaa taaaactatg gatgatgaag
     1501 caagtaaaca attaagcgat agccgtttca agagccttgt aggtgttcag cgcacgagat
     1561 gttagctgtg ttaaaaacag cttatcaacg caacgtaaac gcgcaaaagg tggacgaaaa
     1621 accaaattaa gcctagacga tctcctcatg gtaactattc aatacatgcg agaatagcgc
     1681 acttatgaac aaattgcggc tgattttggc attcacgaaa gcaacttaat ccgtcggagc
     1741 caatgggttg aagtaactct tgttcaaaat ggttttacga tttcaaattc tgccctaatt
     1801 atgaaaaaca gtaaaattcg aaggattgta aggtaagagt ttttttcttt ctgaaaaaat
     1861 ggtataatag caatcaaaac tagaaaataa aacggaattt ggaacagatt tgtctgtatc
     1921 ctagtagagt ggtgatgcta tgaagattag taagaggcac ttattaaatt attccatctt
     1981 gattccctac ttgcttttat ctattttggg cttgattgtg gtctattcga ccaccagtgc
     2041 tattttaatt gaagaaggca agagcgcctt gcagttggtt cgaaaccaag gaatcttttg
     2101 gattgttagt ttgatactga ttgccttaat ttataaattg agactagatt ttttgagaaa
     2161 tgagcgacta atcattttag ttatattaat agaaatgctt ttattgttct tggctcgttt
     2221 tattggtatt tccgtaaacg gggcatacgg ttggatttcg gttgcaggag taactattca
     2281 gccagctgag tacttaaaaa tcattattat ttggtattta gctcaccgat tctccaaaca
     2341 gcaagaagaa atagctactt atgattttca agttttgact caaaatcaat ggcttccccg
     2401 tgcttttaat gattggcgat tcgttctcct agttctgatt ggaagtttgg gaattttccc
     2461 tgatttagga aatgcgacta ttttagtctt ggtttccttg attatgtata cagttagtgg
     2521 aatcgcttat cgctggtttt caaccattct ggcgctcgta tctgccgctt ctgtctttgt
     2581 cttgaccact atcagcctaa tcggtgttga gaccttttca aaaattccag tatttggcta
     2641 tgtagccaag cgctttagtg ccttttttaa tccttttgcc gatcgtgctg atgcaggtca
     2701 ccagttagct aattcttatt ttgccatggt caatggtggt tggtttggtc taggtcttgg
     2761 aaactcgatt gaaaaacgag gttatttgcc agaagctcat acagactttg tcttttctat
     2821 cgtgattgaa gaatttggct ttgctggtgc cagtcttatt ttagctctct tgtttttcat
     2881 gattttgcgg attatcttgg tcggtattcg agcggagaat cctttcaatg ccatggttgc
     2941 actcggtgtc ggagggatga tgttggttca ggtatttgtc aatatcggag ggatttcggg
     3001 cttgattcca tctacaggag taaccttccc cttcttatcc cagggtggaa atagtcttct
     3061 agtcttatca gtggcagtag cctttgtctt aaatattgat gccagtgaaa aacgcgctaa
     3121 gttgtaccga gaattggaaa atcaaccaat gaaccttctg ttgaagtagg ataaagaaag
     3181 gatagtttat gtctcttcaa aaattagaaa attatagtaa taaaagtgtt gtgcaagaag
     3241 aagtcttgat tctaacagaa ttactggaag atattactaa aaatatgctt gccccagaga
     3301 cctttgaaaa aataatacag ttgaaagaat tatcaacgca ggaagattat caaggtctaa
     3361 accgtctagt gactagctta tcaaatgatg aaatggtcta tatttcacgc tatttctcta
     3421 tcttacctct tttgatcaat atttcagagg atgtggattt agcttatgaa atcaatcatc
     3481 aaaataatat tgatcaggac tatttaggta aattatctac aacgattaaa ttggtagcag
     3541 aaaaggaaaa tgccgttgag atcctagaac acttgaatgt tgtccctgtt ttgacagccc
     3601 atccaacaca agtgcaacgc aaaagtatgt tggatttaac aaatcatatt catagtcttt
     3661 tgcgtaaata ccgtgatgtt aagttggggt tgatcaataa agataaatgg tacaatgatt
     3721 tgcgtcgtta catcgaaatt atcatgcaga cagacatgat tcgtgagaaa aaattaaaag
     3781 tgactaacga aatcacgaat gctatggaat attataacag ctcctttttg aaagctgtac
     3841 ctcatttgac gacggagtat aagcgcttag cgcaagcgca tggtctgaat ttaaaacagg
     3901 ctaaaccaat caccatgggt atgtggatag gtggtgaccg tgatggaaat ccatttgtta
     3961 cagcagagac cttgaagcag tctgcactca ctcaatgtga agtcatcatg aactactatg
     4021 ataaaaagat ttaccaactt tatcgtgaat tttctctttc aactagcatt gtcaacgtca
     4081 gcaagcaagt cagagaaatg gctcgtcaat ccaaggataa ctcgatttac cgcgaaaaag
     4141 agctttaccg tcgtgccttg tttgatattc aatcaaaaat tcaggcaact aaaacctatc
     4201 tgattgagga tgaagaagtt gggactcgtt atgaaaccgc caatgatttc tacaaggatt
     4261 tgattgccat tcgagattct ctactagaaa ataagggtga gtccttgatt tcaggtgatt
     4321 ttgtggaatt attgcaggca gtagagatat ttggttttta cctagcatca attgatatgc
     4381 gacaagactc tagcgtctat gaagcctgtg tggcagaact cttgaaatca gcaggaattc
     4441 attctcgtta tagcgagttg agcgaagaag aaaagtgtga ccttctcttg aaagaattag
     4501 aagaagatcc ccgaattctt tctgcgactc acgcagaaaa atcagaatta ttagcaaaag
     4561 aattagctat ttttaagacg gctcgtgttt tgaaagataa gttgggagat gatgtcatcc
     4621 gtcagaccat catttcacat gcaaccagcc tttctgatat gctagaatta gctattctgt
     4681 taaaagaagt aggactggtg gatacggaaa gggcgcgtgt tcagattgtt cccctttttg
     4741 aaacaattga agacttggat cattcagaag aaacaatgag aaaatatctt tctcttagcc
     4801 ttgccaaaaa atggattgac tcacgaaata actaccaaga aatcatgctt ggctactctg
     4861 acagtaataa agatggcggt tacttgtcat catgttggac cctctacaag gctcaacaac
     4921 aattgactgc tattggagat gaatttggcg ttaaggttac cttcttccat ggtcgtggtg
     4981 gtactgtcgg tcgtggtggt gggccaacct atgaagccat tacatctcaa ccgctcaagt
     5041 ctatcaagga tcgtatccgc ttgacggagc agggtgaagt aattgggaat aaatacggta
     5101 acaaagacgc cgcttactat aaccttgaaa tgctagtatc ggcagctatt aaccgtatga
     5161 ttactcagaa gaagagcgat accaataccc caaatcgtta tgaagccatt atggatcaag
     5221 tagtggaccg tagttacgat atctaccgtg atttggtctt tggtaatgag catttctatg
     5281 attatttctt cgagtcaagt ccaatcaagg ctatttcaag ttttaatatt ggttctcgtc
     5341 cagccgctcg taagactatt actgaaatcg gtggtttgcg tgccatccct tgggtattct
     5401 catggtcaca gagtcgtgtt atgttccctg gatggtacgg ggttggttca agcttcaagg
     5461 aatttatcaa taaaaatcca gagaatattg ctatcttacg agatatgtac caaaattggc
     5521 ctttcttcca atcgcttctt tcaaatgttg atatggtttt gtcaaaatca aatatgaata
     5581 ttgcttttga atatgctaaa ctttgtgaag acgagcaagt taaggccatc tatgagacta
     5641 ttttaaatga atggcaagtt actaagaacg ttatcttggc tattgaagga catgacgaac
     5701 tcttagctga caatccatat ctaaaagcta gtctggatta ccgtatgcct tactttaata
     5761 ttctcaacta tattcagtta gagttgatta aacgccaacg tcgtggagaa ttgtccagtg
     5821 atcaagaacg attgattcat atcaccatca acggaattgc gacaggattg cgtaattcag
     5881 gttgataatt ttcaagagtg aatgctaaaa gtgaatatca aaaaaattct aatagactat
     5941 tgacaagtag tttaaaaatg atataattta accattcaga aaagtaatca tacaaacttt
     6001 ttagagagtc tgtggtagct gaaaacagat aagtggcgat gatgaaaatt gggctgaatg
     6061 ctatttagaa tttgaaatta taaaaattcg gtaagcacac cttacagtgc atctcgttat
     6121 tgcgagactg agcgataggg aaattcccta taattgaggt ggtaccgcgc atcgacgtcc
     6181 tcacacaagt tttttgtgtg aggatttttt tgatggaggt tagtatggaa agaaaacgat
     6241 ggcgtcgctt gtttagataa gtgaaatatg ttaaaggaaa taaaaaggag aaacagaatg
     6301 aaaaataaac gtttaattgg aattattgct gcattagcag tcttagtagc aggaagcttg
     6361 atttattctt caatgaataa atcagaagct cagaataata aggatgagaa gaaaataact
     6421 aagattggtg tgcttcaatt tgtgagccat ccatcccttg atttgattta taaagggatc
     6481 caagatggac ttgcagaaga aggatataaa gatgatcaag ttaaaattga ttttatgaac
     6541 tcagaaggtg accaaagtaa ggttgcgaca atgagtaaac aattggttgc aaatgggaat
     6601 gaccttgtgg ttggtatcgc aacaccagca gcccaagggt tggctagtgc aacaaaagac
     6661 ctaccggtta tcatggccgc tattacagac ccaattggtg ctaacttggt taaagatttg
     6721 aaaaaaccag gtggcaacat tacaggggta tctgaccaca atccagctca acaacaagtt
     6781 gaactcatca aggctctgac accgaatgtg aaaacaatcg gagctcttta ctcaagtagc
     6841 gaagacaatt caaaaacaca ggtcgaagaa tttaaggctt atgctgaaaa agcaggtctg
     6901 acagtggaaa catttgcagt tccttcaaca aatgaaattg cctcaactgt cactgttatg
     6961 actagcaagg tagatgctat ttgggttcca attgataaca ccattgcatc aggatttcca
     7021 acggttgtct ctagcaatca aagttctaag aaaccaattt atcccagtgc gacagctatg
     7081 gtagaagtag gtggtttggc atcagttgta attgaccaac atgaccttgg tgtggcaaca
     7141 ggtaaaatga ttgtgcaagt cttgaaaggt gcaaaaccag ccgatacccc agtcaatgtc
     7201 ttttcaactg gtaagtcagt catcaataaa aaaatagcac aagaactagg tattactatt
     7261 cctgagtctg ttctcaaaga agcaggacaa gtcatcgaat aaataaggta gggaggagga
     7321 gactcttccc attttttaaa aagatgaata tagaaaggat taaataaaat atgattgttt
     7381 ccattatttc tcaaggattt gtctgggcta ttctaggtct gggaatcttt atgacattta
     7441 ggattttaaa ctttccagat atgacgacag aaggttcctt ccctcttggg ggagctgttg
     7501 ctgtcacttt gataaccaaa ggcgtgaacc catttttagc gacacttgtt gctgtaggag
     7561 caggttgttt ggctggaatg gcagcaggcc ttctttatac aaaagggaag atcccaacct
     7621 tgctctcagg gattttggtg atgacttctt gtcactcaat catgctcttg attatgggac
     7681 gtgcgaattt aggcctgctt ggaaccaagc aaattcagga tgttttgcct tttgattcgg
     7741 atttgaatca actcttgaca ggtctcatct ttgtgagtat tgttattgct ctcatgctct
     7801 ttttcttgga cactaaactc ggacaagcct atattgctac aggggataat cctgatatgg
     7861 ctagaagttt cgggattcat actggacgca tggagctcat gggcttggtc ttatcaaatg
     7921 gtgtgattgc ccttgcaggt gccctcattg ctcagcaaga aggttatgcc gatgtgtctc
     7981 gagggatcgg ggttatcgtt gtggggcttg caagtttgat tattggagaa gttattttca
     8041 agagtttgag cttggcagag cgtttggtta ctatcgttgt aggttctatc gcttatcaat
     8101 ttttagtgtg ggcagttatc gcacttggct ttaatacaag ttaccttcgt ttatacagtg
     8161 ccttgatttt agcagtctgc ctcatgattc caacatttaa gcaaacaatc ttgaaaggag
     8221 ccaagttaag caaatgacag caattgtaga attaaaaaat gcaaccaaaa tcgttaaaaa
     8281 tggctttgat gaagaaaaga ttattttaaa tgatgtttcc ttagaaattt ttgaacggga
     8341 ctttatcacg attttgggcg gaaatggtgc tggaaaatca accctcttta acactatagc
     8401 agggacctta tcactaacta gtggaactat ccgtatttta ggtgaagaac tcactaagtt
     8461 ttcacccgag aagcgtgcca agtacctgtc tcgtgtcttc caagatccaa agatggggac
     8521 agctccccgt atgacggtcg ctgaaaatct tttaatcgcc aagtttcgtg gtgaaaagcg
     8581 tggattgtta ccacgacgct tgactagcta taaggatgaa tttcaggcaa ccattgaaaa
     8641 agtaggaaat ggtcttgaga aacacttgaa tacaccgatt gagttcttat caggtggaca
     8701 aagacaggct ttgagtctct tgatggcaac cttgaagcga cctgaattac tcctgttaga
     8761 tgagcatact gctgccctgg atccaaagac tagtgttgct ttgatggaat tgacagatga
     8821 atttgttaag aaagatcagc taacagccct tatgattact catcatatgg aagatgctct
     8881 caaatacggc aatcgcttga ttgtcatgaa agaaggacgg attatccaag atttaaacca
     8941 agaagaaaaa gcaaaaatga aaatctctga ttattatcaa ctctttgaat aaagtaggga
     9001 aaatgagtga aatggtttac tttttttctg aaataaagta tactatataa agtaaactat
     9061 gataacatgg aggtattgtg tatggttgac aaacaagtca ttgaagaaat caaaaacaat
     9121 gccaacattg tggaagtcat aggagatgtg atttctttac aaaaggcagg acggaactat
     9181 ctagggctct gtccttttca tggtgaaaaa acaccttctt tcaacgttgt agaggataag
     9241 cagttttatc actgtttcgg ttgtggtcgc tcaggtgatg tctttaagtt catcgaggag
     9301 taccaagggg ttccctttat agaggcagtc caaatcttag gtcagcgtgt tggaattgag
     9361 gttgaaaaac cgctttatag tgagcagaag ccagcctcac ctcaccaagc tctttatgat
     9421 atgcacgaag atgcagccaa attttaccat gcgattctca tgacaacgac tatgggcgaa
     9481 gaggccagaa attaccttta tcagcggggg ttgacagatg aagtgcttaa acatttttgg
     9541 attggtttag cacctccaga acgaaactat ctctatcaac gtttgtctga tcagtatcgt
     9601 gaagaggatt tactggattc aggcctgttt tatctctcgg atgccaatca atttgtagac
     9661 acctttcaca atcgcattat gtttcccctg acaaatgacc aaggaaaggt cattgccttc
     9721 tcaggtcgta tctggcaaaa aacggattca caaacttcta agtataaaaa cagccgatcg
     9781 actgcaattt ttaacaaaag ttacgaatta tatcatatgg atagggcaaa aaaatcttct
     9841 ggaaaagcta gtgagattta cttgatggaa ggattcatgg atgttattgc agcctatcgg
     9901 gctggaatcg aaagtgctgt ggcgtcgatg ggaacggcct tgagtcgaga gcatgttgag
     9961 catctgaaaa ggttaaccaa gaaattggtt cttgtttacg atggagataa ggctgggcaa
    10021 gccgcgacat tgaaagcatt ggatgaaatt ggtgatatgc ctgtgcaaat cgtcagcatg
    10081 cctgataact tggatcctga tgagtatcta caaaaaaatg gtccagaaga cttggcctat
    10141 ctattaacga aaactcgtat tagtccgatt gagttctaca ttcatcagta caaacctgaa
    10201 aacagtgaaa atctgcaggc tcagattgag tttcttgaaa aaatagctcc cttgattgtt
    10261 caagaaaagt ccatcgctgc tcaaaacagc tatattcata ttttagctga cagtctggcg
    10321 tcctttgatt atacccagat tgagcagatt gttaatgaga gtcgtcaggt gcaaaggcag
    10381 aatcgcatgg aaggaatttc cagaccgacg ccaatcacca tgccaatcac caagcagtta
    10441 tcggctatta tgagggcaga agcccatcta ctctatcgga tgatggaatc ccctcttgtt
    10501 ttgaacgatt accgtttgcg agaagacttt gcatttgcta cacctgaatt tcaggtctta
    10561 tatgacttgc ttggccagta tggaaatctt cctccagaag ttttagcaga gcagacagag
    10621 gaagttgaaa gagcttggta ccaagtttta gctcaggatt tgcctgctga aatatcgccg
    10681 caggaactta gtgaagtaga gatgactcga aacaaggctc tcttgaatca ggacaatatg
    10741 agaatcaaaa agaaggtgca ggaagctagc catgtaggag atacagatac agccctagaa
    10801 gaattggaac gtttaatttc ccaaaagaga agaatggagt aataatggca acaaaacaaa
    10861 aagaagtaac aacatttgac gtacaggtag cagaatttat ccgtaatcat aagcaaaaag
    10921 ggacagcaac agatgatgaa atcaatgcaa gtctggttat tccttttacc ttggacgctg
    10981 atgggattga agatctcttg caacggattc aggatgcagg aatttctatc acagataacg
    11041 aaggaaatcc aagtgcgcgt gttcttagca atgaagaaga accagaactc agcgatgagg
    11101 acttgattgg gtcaacttct gctaaggtca atgaccctgt ccgtatgtac ttgaaagaaa
    11161 taggggtcgt tcctctcttg accaatgaag aggagaaaga gttggcactg gctgttgaag
    11221 ctggtgatat cgaagccaaa caacgtcttg cggaagccaa tcttcgtttg gttgtttcca
    11281 ttgccaaacg ctatgtcggt cgtggtatgc agttccttga cttgattcaa gaaggaaata
    11341 tgggcttgat gaaggcggtt gacaagtttg actattctaa agggttcaag ttttcaactt
    11401 atgcaacttg gtggattcgt caggctatca ctcgtgctat tgcggaccaa gctcgtacca
    11461 tccgtatccc agttcacatg gttgaaacta tcaataaatt ggttcgtgaa cagcggaatc
    11521 tccttcaaga attggggcaa gatccgacac cagaacagat tgctgaacga atggatatga
    11581 cacctgataa ggttcgtgaa atcttgaaga ttgcccaaga accagtatct cttgaaactc
    11641 ctatcggtga agaggacgat agccaccttg gagactttat cgaagatgaa gtgattgaaa
    11701 atccagtgga ttatacgact cgtatcgtct tgcgtgagca attggatgaa atcttagata
    11761 ctcttacaga ccgtgaagaa aatgttctgc gtctacgttt tggactagat gatggaaaaa
    11821 tgcgcacact tgaagatgtg gggaaagtct ttaacgtaac tcgtgagcgt atccgtcaga
    11881 ttgaagcaaa ggctttgaga aaactacgcc aaccaagtcg tagcaaaccg cttcgtgatt
    11941 ttattgaaga ctaagagtga ggaaaatatg gcttatacag aagagcaaat tgaaaacatc
    12001 aaaacacgga ttttaacagc cttggaagaa gtcatcgacc ctgagttggg aatcgatatt
    12061 gtcaatcttg gtttgatcta tgagattcgt tttgacggtg acacagggca aacagagatt
    12121 gacatgactt tgacaactat gggttgtccc ttagcagacc ttttgacaga ccagatttat
    12181 gatgctatga tagaggttcc agaagtaacg gatactgagg ttaaattagt ctggtatcca
    12241 gcctggacag ttgaaaaaat gagtcgctat gcccgcattg ccctaggcat taagtaaaca
    12301 tataaaagca tgtgagagac tgttggaaaa gtgaggaaat ttcccctctt ttcctctagt
    12361 ctctcctttc ttttgctgat tttattcaaa gaaaatgata taatagtagt tatggagaaa
    12421 aagaaattac gcatcaatat gttgagttca agtgagaaag tagcaggaca gggagtttca
    12481 ggtgcttacc gtgaattagt tcgtcttctt caccgtgctg ccaaggacca attgattgtt
    12541 acagaaaatc ttccaatcga ggcagatgtg actcactttc atacgattga ttttccctat
    12601 tatttatcaa ccttccaaaa gaaacgctca gggagaaaga ttggctatgt gcatttcttg
    12661 ccagctacac ttgagggaag tttgaaaatt ccatttttct taaagggaat tgtgaaacgc
    12721 tatgtatttt ctttttacaa ccggatggag cacttggttg tggtcaatcc tatgtttatt
    12781 gaggatttgg tagcagctgg tattccacgt gaaaaagtga cctatattcc taactttgtc
    12841 aacaaggaaa aatggcatcc tctaccacaa gaagaggtag ttagactgcg cacagatctt
    12901 ggtcttagtg acaatcagtt tatcgtagta ggtgctgggc aagttcagaa acgtaaaggg
    12961 attgatgact ttatccgtct ggctgaggaa ttgcctcaga ttacctttat ctgggctggt
    13021 ggcttctctt ttggtggtat gacagatggt tatgaacact ataagaaaat tatggaaaat
    13081 ccccctaaaa atttgatttt tccaggcatt gtatcgccag agcggatgcg cgaattgtat
    13141 gctctagcgg atcttttctt gttgcctagt tacaatgagc tctttcctat gactatttta
    13201 gaagctgcga gttgtgaggc tcctattatg ttgcgtgatt tagatctcta taaggtgatt
    13261 ttggagggaa attatcgggc gacagcgggt agagaagaga tgaaagaggc tattttggaa
    13321 tatcaagcaa atcctgctgt cttaaaagat ctcaaagaaa aggctaagaa tatttccaga
    13381 gagtattctg aagagcatct gttacaaatc tggttggact tttatgagaa acaagccgct
    13441 ttagggagaa agtaaaaagt gaggtaatct atgcgaattg gtttatttac agatacctat
    13501 tttcctcagg tttctggtgt tgcgaccagt attcgaacct tgaaaacaga acttgaaaag
    13561 cagggacatg ctgtttttat ctttacgacg acagataagg atgtcaatcg ctacgaagat
    13621 tggcaaatta tccgcattcc aagtgttcct ttctttgctt ttaaggatcg tcgctttgcc
    13681 taccgaggtt ttagcaaggc acttgaaatt gctaaacagt atcagctaga tattatccat
    13741 actcagacag aattttctct tggcctgttg gggatttgga ttgcgcgtga attgaaaatt
    13801 ccagtcatcc atacctatca cacccagtat gaagactatg tccattatat tgctaagggg
    13861 atgttgatcc ggccgagtat ggtcaagtat ctggttagag gtttcctgca tgatgtggat
    13921 ggggttattt gccctagtga gattgtccgt gacttgctat ctgattataa ggtcaaggtt
    13981 gaaaaacggg tcattcctac tgggattgaa ttagccaagt ttgagcgtcc ggaaatcaag
    14041 caggaaaatt tgaaagaact gcgtagtaaa ctagggattc aagatggtga aaagacgttg
    14101 cttagtcttt cgagaatctc ctatgaaaaa aatattcaag cagttttagc agcctttgct
    14161 gatgttctga aagaggaaga caaggttaaa ctggtagtag ctggggatgg cccttatctg
    14221 aatgacctca aagagcaagc tcagaaccta gagattcaag actcagtcat ctttacaggg
    14281 atgattgctc ctagtgagac ggctctttac tataaagcgg cggatttctt catttcggca
    14341 tcgacaagcg aaacgcaagg tttgacctac ttggaaagct tagccagtgg aacacctgtc
    14401 attgctcacg gaaatcctta tttgaacaac ctcatcagtg ataaaatgtt tggaaccttg
    14461 tactatggag aacatgattt ggctggtgct attttggaag ccctgattgc aacaccagac
    14521 atgaacgagc ataccttatc agagaaattg tatgagattt cagctgagaa ctttgggaaa
    14581 cgagtgcatg agttttatct ggatgccatt atttcaaata acttccagaa agatttggct
    14641 aaagatgata cggtcagtca gcgtatcttt aagacagttt tgtatcttca gcaacaggtg
    14701 gttgctgtac ctgtaaaagg atctagacgc atgttgaagg cttcaaaaac acagttgatc
    14761 agtatgagag actattggaa agaccatgaa gaatagaaag aggaacagct atgaaaaaaa
    14821 caattaatga gaagcggtcg tgataaaaag attgcgggtg tttgtgctgg ggtggcccat
    14881 tatctggata tggatccgac tatcgttcaa gtcatttggg gtgttcttac ttgctgttac
    14941 ggagctggaa ttgtagctta cattatttta tggattatcg caccagtagc aacagactat
    15001 tgaaaactag ctcaattcat gctaagataa tagaaaatag aatgtaacga aggagagaaa
    15061 aatggcattt ggagataatg gaaatcgtaa aaaaactatg tttgagaaaa taaccttgtt
    15121 tatcgtgatt atcatgctag tagcaagttt attgggaatt tttgcaactg caattggtgc
    15181 cctcagtaat ctataaaata gattcaagaa aatttagtga ctgggatttc ccagcccttt
    15241 tttaaagtga gaagaaataa tgagtatgtt tttagataca gctaagatta aggtcaaggc
    15301 tggtaatggt ggcgatggta tggttgcctt tcgtcgtgaa aaatatgtcc ctaatggagg
    15361 cccttggggt ggtgatggtg gtcgtggagg caatgtggtc ttcgttgtag acgaaggact
    15421 acgtaccttg atggatttcc gctacaatcg tcatttcaag gctgattctg gtgaaaaagg
    15481 gatgaccaaa gggatgcatg gtcgtggtgc tgaggacctt agagttcgag taccacaagg
    15541 tacgactgtt cgtgatgcgg agactggcaa ggttttaaca gatttgattg aacatgggca
    15601 agaatttatc gttgcccacg gtggtcgtgg tggacgtgga aatattcgtt tcgcgacacc
    15661 aaaaaatcct gcaccggaaa tctctgaaaa tggagaacca ggtcaggaac gtgagttaca
    15721 attggaacta aaaatcttgg cagatgtcgg tttagtagga ttcccatctg tagggaagtc
    15781 aacactttta agtgttatta cctcagctaa gcctaaaatt ggtgcctacc actttaccac
    15841 tattgtacca aatttaggta tggttcgcac ccaatcaggt gaatcctttg cagtagccga
    15901 cttgccaggt ttgattgaag gggctagtca aggtgttggt ttgggaactc agttcctccg
    15961 tcacatcgag cgtacacgtg ttatccttca catcattgat atgtcagcta gcgaaggccg
    16021 tgatccatat gaggactacc tagctatcaa taaagagctg gagtcttaca atcttcgcct
    16081 catggagcgt ccacagatta ttgtagctaa taagatggac atgcctgaga gtcaggaaaa
    16141 tcttgaagaa tttaagaaaa aattgtctga aaattatgat gaatttgaag agttaccagc
    16201 tatcttccca atttctggat tgaccaagca aggtctggca acacttttag atgctacagc
    16261 tgaattgtta gacaagacac cagaattttt gctctacgac gagtccgata tggaagaaga
    16321 agcttactat ggatttgacg aagaagaaaa agcctttgaa attagtcgtg atgacgatgc
    16381 gacatgggta ctttctggtg aaaaactcat gaaactcttt aatatgacca actttgatcg
    16441 tgatgaatct gtcatgaaat ttgcccgtca gcttcgtggt atggggggtg atgaagccct
    16501 tcgtgcgcgt ggagctaaag atggggattt ggtccgcatt ggtaaatttg agtttgaatt
    16561 tgtagactag gagactggta tgggagataa accgatatct ttccgagatg cggatggtaa
    16621 ttttgtttcc gccgcagacg tttggaatga aaagaaattg gaagaactat ttaatcgtct
    16681 caatccaaat cgtgccttga gattggcacg aactaaaaag gaaaatccat ctcagtaaag
    16741 aagctaaaaa atcccgtgcc tcatcagaca cgggattttg tggtacgaca ggcatgtata
    16801 gcaaactgaa tctggaatag cacagcatat cttctaaaat attgctataa attgatttga
    16861 atttcaaaat taaattgttt tattcttatt tcaatttgtt atagtatatc tgatgtcaaa
    16921 gttctcggcg agtcaaatag tgattcccaa gcctgactat cgtgaggtag cggattaaaa
    16981 tggtctgggg atagaccgtt ttaagtctga tgctggaaat aagaattgtc agaagaaggg
    17041 atagcgaaat cgtggctcta cgaacaggaa cgtgataata aggcgtatat agcgaataag
    17101 agggcctcaa actctaaagt ccaaaaaggt agtcgtaacc tatatgcgta aatcatgaga
    17161 gtaattgaat tcgtactaag attttctatt tttactgtaa ccttttaacg tcctcatatc
    17221 ttgtatacac gaggaaagat gtacgactta tcccgtgagg tctatcacta taaagagaaa
    17281 acgacagata gaagtgatcc tgagtcacgg ttatctttaa aatagattgt ctgataggat
    17341 gctagcctga aaacgtcttg ttcatgaaat ataggacggt atgtataaaa cgcttctgtg
    17401 aactgagaga agggggagaa gttcttgcta aaatttagtt gaacagccgt attccgatac
    17461 ttagataaga gatctagtct tagctcctac tcagtttcag gggataaaaa aggggcaata
    17521 gcgattcgag aaagattata ctcttcgaaa atctcttcaa atcacgtcaa tatcgccttg
    17581 ccgtatgtgt aggatactaa ctacgtcagt tccatctaca atctcaaaac agtgttttga
    17641 gcaacctgcg gctagtttcc tagtttgatc tttgattttt attgagtatt agtaattcag
    17701 ttactaactc gtcaactctg atttatccaa taaaattgaa aaggatggaa aaaaggataa
    17761 atttatgata tactttattt tgaagacctt attagaaatc ttgaaagagt attgaaaact
    17821 tagaatgaga aaaattgtta tcaatggtgg attaccactg caaggtgaaa ttactattag
    17881 tggtgctaaa aatagtgttg tggccttaat tccagctatt atattggctg atgatgtggt
    17941 gactttggat tgtgttccag atatttcgga tgtagccagt cttgtcgaaa tcatggaatt
    18001 aatgggagct actgttaagc gttatgacga tgtcttggag attgatccaa gaggtgttca
    18061 aaatattcca atgccttatg gtaaaattaa cagtcttcgt gcatcttact atttttatgg
    18121 gagcctctta ggccgttttg gtgaagcgac agttggtcta ccgggaggat gtgatcttgg
    18181 tcctcgtccg attgacttac accttaaggc gtttgaagct atgggtgcca ctgctagcta
    18241 cgagggagat aacatgaagt tatctgctaa agatacagga cttcatggtg caagtattta
    18301 catggatacg gttagtgtgg gagcaacgat taatacgatg attgctgcgg ttaaagcaaa
    18361 tggtcgtact attattgaaa atgcagcccg tgaacctgag attattgatg tagctactct
    18421 cttgaataat atgggtgccc atatccgtgg ggcaggaact aatatcatca ttattgatgg
    18481 tgttgaaaga ttacatggga cacgtcatca ggtgattcca gaccgcattg aagctggaac
    18541 atatatatct ttagctgctg cagttggtaa aggaattcgt ataaataatg ttctttacga
    18601 acacctggaa gggtttattg ctaagttgga agaaatggga gtgagaatga ctgtatctga
    18661 agacagcatt tttgtcgagg aacagtctaa tttgaaagca atcaatatta agacagctcc
    18721 ttacccaggc tttgcaactg atttgcaaca accgcttacc cctcttttac taagagcgaa
    18781 tggtcgtggt acaattgtcg atacgattta cgaaaaacgt gtaaatcatg tttttgaact
    18841 agcaaagatg gatgcggata tttcgacaac aaatggtcat attttgtaca cgggtggacg
    18901 tgatttacgt ggggccagtg ttaaagcgac cgacttaaga gctggggctg cactagtcat
    18961 tgctgggctt atggctgaag gtaaaactga aattaccaat atcgagttta tcttacgtgg
    19021 ttattctgat attatcgaaa aattacgtaa tttaggagcg gatattagac ttgttgagga
    19081 ttaaaccgta gaggtgttta tgaatatttg gaccaaatta gcaatgtttt ctttttttga
    19141 aacggatcgc ttgtatttgc gtcctttctt ttttagtgat agtcaggact tccgcgagat
    19201 agcttcaaat ccagaaaatc ttcaatttat tttcccaacg caggcaagtc tggaagaaag
    19261 tcaatatgca ctggccaatt actttatgaa gtcccctttg ggagtgtggg caatttgtga
    19321 ccagaaaaat caacaaatga ttggttctat taaatttgag aagttagatg aaatcaaaaa
    19381 agaagctgag cttggctatt ttttgagaaa agatgcttgg tcgcaaggat ttatgacaga
    19441 ggttgttaga aaaatttgtc agctttcttt tgaggaattt ggcttaaaac aattatttat
    19501 cattacccac cttgaaaatg aagctagcca aagagttgct cttaagtctg gatttagttt
    19561 gttccgtcag tttaagggaa gtgatcgtta cacaagaaaa atgcgggatt atcttgaatt
    19621 tcggtatgta aaaggagagt tcaatgagta agcatcagga aattctaagc tatttggagg
    19681 aattaccagt aggtaaaagg gtcagtgttc gtagcatttc gaatcatcta ggagttagtg
    19741 atggaacagc ctatcgagct attaaagaag ctgaaaaccg tggaattgtg gagacccgtc
    19801 ctagaagtgg aacaattcgt gttaaatccc agaaagttgc tatagagaga ttaacgtttg
    19861 ctgaaattgc agaagtgact tcttctgagg ttctggctgg gcaagaaggt ttagagagag
    19921 agtttagtaa gttttcaatt ggtgccatga ctgaacaaaa tatcttgtct taccttcatg
    19981 atggggggct cttgattgtc ggagaccgaa cccgtattca gttgctagcc ttggaaaatg
    20041 aaaatgcagt tctggttaca gggggatttc aggttcatga tgatgtgctt aaactggcca
    20101 atcaaaaagg gattcctgtt ctaagaagta agcatgatac ctttaccgtc gcgaccatga
    20161 tcaataaagc cttgtcaaat gtccaaatca agactgatat tctgacagtt gagaaacttt
    20221 atcgccctag tcatgagtat ggttttctga gagagacaga tacagttaaa gattatttgg
    20281 acttggttcg taagaatcgt agcagccgtt tccctgttat caatcaacat caggtcgttg
    20341 ttggcgttgt aaccatgaga gacgctggtg ataaatcacc aagcacgaca attgataagg
    20401 ttatgtctcg tagtctattt ttggttgggt tatcgacaaa tattgccaat gtgagtcaac
    20461 ggatgatcgc agaagacttt gaaatggtac cagttgttcg aagcaatcaa actttgcttg
    20521 gcgttgtgac gcgacgagat gtcatggaga agatgagccg ttcccaagtt tcggctctac
    20581 caactttttc tgagcagatt ggacaaaagc tctcttatca ccatgatgaa gtagtcatta
    20641 cagtggaacc ctttatgcta gaaaaaaatg gagttttggc taatggtgta ttggcagaaa
    20701 ttctgaccca catgacccaa gatttagttg ttaatagtgg tcgcaatctc attatcgagc
    20761 agatgctgat ctactttttg caggctgttc agatagatga tatattgcgc attcaggcac
    20821 ggattattca tcatacgaga cggtcagcta taattgatta cgatatttat catggtcacc
    20881 agattgtttc aaaagcaaat gtgactgtta aaattaatta gaaactagga gaaaagatga
    20941 taacattaaa atcagctcgt gaaatcgaag ctatggacaa ggctggtgat tttctagcaa
    21001 gtattcatat aggcttacgt gatttgatta agccaggcgt agatatgtgg gaagttgaag
    21061 aatatgtccg ccgtcgttgt aaagaagaaa atttccttcc acttcagatt ggggttgacg
    21121 gtgccatgat ggactatcct tatgctacct gttgctctct taacgatgaa gtggctcacg
    21181 ctttccctcg tcattatatc ttgaaagatg gtgatttgct caaagttgat atggttttgg
    21241 gaggtcccat tgctaaatct gacctgaatg tctcaaaatt aaacttcaac aatgttgaac
    21301 aaatgaaaaa atacactcag agctattctg gtggtttagc agactcatgt tgggcttatg
    21361 ctgttggtac accgtccgaa gaagtcaaaa acttgatgga tgtaaccaaa gaagctatgt
    21421 acaagggtat tgagcaagct gttgttggaa atcgtatcgg tgatatcggt gcggctattc
    21481 aagaatacgc tgaaagtcgt ggttacggtg tagtgcgtga tttggttggt catggtgttg
    21541 gcccaactat gcacgaagaa ccaatggttc ctaactatgg tattgcaggt cgtggactcc
    21601 gtcttcgtga aggaatggtc ttaaccattg aaccaatgat caatacaggc gattgggaaa
    21661 ttgatacaga tatgaaaact ggttgggcgc ataagaccat tgacggtgga ttgtcatgtc
    21721 agtatgaaca ccaatttgtc attacgaaag atggacctgt tatcttgact agccaaggtg
    21781 aagaaggaac ttattaataa aaagtgaaaa gactactgga agtttatttt gataaaaaat
    21841 ccagtagatc ttttcataat aaaacgcatt gtatcaagtg ttaggagctg atatcatgcg
    21901 tttttctgct tttaagattt tttccaactc tgtttgtaag cgcatcataa caaagggtct
    21961 aggattcagg gctctcctcc tatatactat tagtaaagta aaactaaggg aggatatttt
    22021 agtgtcgcag tctattgttc ctgtagagat tccacaatat tgtcgttttg attctaaaaa
    22081 gagaaatgga attctgttta acgttcgtat tgccaatctt aaatttacct tttttagatt
    22141 agactccctg cgaaacaaaa tatggtatag tagttctatg aatgatgaag caagtaaaca
    22201 actaactgat gcacgattta agcgtcttgt tggtgttcag cgcacgactt ttgaagagat
    22261 attagctgta ttaaaaacag cttatcaact taaacacgca aaaggtggac gaaaacctaa
    22321 attaagccta gaagaccttc ttatggccac tcttcactat gtgcgagaat atcgaactta
    22381 tgaagaaatt gcggctgatt ttggtatcca cgaaagcaac ttactccgtc ggagccaatg
    22441 ggttgaagta actcttgttc aaaggtggtg ttacgatttc aagaactcct ctcagctctg
    22501 aggacacggt aatgattgat gcgacggaag taaaaatcaa tcgccctaaa aaagaattag
    22561 cgaatcattc tggtaaaaag aaatttcacg ctatgaaggc tcaagcgatt gtcacaagtc
    22621 aagggagaat tgtttctatt gtcatgatat gaagttgttc aaaatgagtc gcagaaatat
    22681 tggacaagct ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc
    22741 tcaagcacaa actccacgta aatccagcaa actcaagcca ctaatagctg aagataaagc
    22801 ttataaccat gcgctatcta aggagagaag caaggttgag aacatctttg ccaaagtaaa
    22861 aacgtttaaa atgttttcaa caacctatcg aagtcatcgt aaacgcttcg gattacgaat
    22921 gaatttgagt gctggtatta tcaatcatga actaggattc tagttttgca ggaagtctat
    22981 tgaggtattg agctagttta tgaaaaaatt gggtgaaaag tcgagtgttt tagaaaccca
    23041 cagtgtagta ttctagtttc aatccactat attttgctac tccccgtaaa gtttctattt
    23101 tccctgattt ctgatataat agaaatattg acttcaagag taaggaagag aagatgaacg
    23161 cattattaaa tggaatgaat gaccgtcagg ctgaggcggt gcaaacgaca gaaggtccct
    23221 tgctaatcat ggcaggggct ggttctggaa agactcgtgt tttaacccac cgtatcgctt
    23281 atttgattga tgaaaagctg gtcaatcctt ggaatatctt ggccattacc tttaccaaca
    23341 aggctgcgcg tgagatgaaa gagcgtgctt atagcctcaa tccagctact caggactgtc
    23401 tgattgcgac cttccactcc atgtgtgtgc gtattttgcg tcgcgatgcg gaccatattg
    23461 gctacaatcg taattttaca attgtggatc ctggtgaaca gcgaacgctc atgaaacgta
    23521 ttctcaaaca gttgaacttg gatcctaaaa aatggaatga acgaactatt ttggggacca
    23581 tttccaatgc taagaataat ttgattgatg atgttgctta tgctgcccaa gctggcgata
    23641 tgtatacgca aattgtggcc cagtgttata cagcctatca aaaagaactt cgtcagtccg
    23701 aatccgttga ctttgatgat ttgattatgc tgaccttgcg tctctttgat caaaatcctg
    23761 atgttttgac ctactaccag caaaaattcc aatacatcca cgttgatgag taccaagata
    23821 ccaaccacgc tcagtaccaa ttggtcaaac tcttggcttc ccgttttaaa aatatctgtg
    23881 tggttgggga tgcggaccag tctatctacg gttggcgtgg tgctgatatg cagaatatct
    23941 tggactttga aaaggattac cccaaagcca aggttgtttt gttggaggaa aattaccgct
    24001 caaccaaaac cattctccaa gcggccaacg aggttattaa aaataataaa aatcgccgtc
    24061 ctaaaaatct ctggactcaa aacgctgatg gggagcaaat cgtttactat cgtgctgatg
    24121 atgagctgga tgaggctgta tttgtagcca gaaccatcga tgaacttagt cgcagtcaaa
    24181 acttccttca taaggatttt gcagttctct atcggactaa tgcccagtcc cgtacaattg
    24241 aggaagccct gctcaagtct aacattcctt ataccatggt tggcggaacc aaattctaca
    24301 gccgtaagga aattcgcgat attattgctt atctcaacct tattgctaat ttgagtgaca
    24361 atattagttt tgagcgtatt atcaacgagc ctaaacgtgg aattggtcta ggtacagttg
    24421 agaaaatccg tgattttgca aatttgcaaa atatgtctat gctggatgct tctgctaata
    24481 ttatgttgtc tggtatcaag ggtaaggcag cccaatctat ctgggatttt gccaatatga
    24541 tgcttgattt gcgggagcag ctagaccact taagcattac agagttggtt gagtccgtcc
    24601 tagaaaaaac aggttatgtc gatattctta acgcccaagc gactctagaa agcaaggcac
    24661 gggttgaaaa tatcgaagag tttctttctg ttacgaagaa ctttgatgac accacggatg
    24721 tgacagaaga ggaaactggt ctggacaaac tgagtcgttt cttaaatgac ttggctttga
    24781 ttgccgacac agattcaggt agtcaggaga catcagaagt gaccttgatg accctgcatg
    24841 ctgccaaagg tctcgaattt ccagttgtct ttttgattgg gatggaagaa aatgtctttc
    24901 cacttagtcg tgcgactgaa gatccagatg aattagaaga agagcgccgt ctagcctatg
    24961 taggtatcac gcgtgcagag aaaattctct atctgaccaa tgccaactca cgcttgcttt
    25021 ttggtcgtac caattataac cgtccgactc gttttattaa cgaaatcagt tcagacttgc
    25081 ttgagtatca aggtctggct cgtcctgcaa atacaagctt taaggcatca tatagcagtg
    25141 gtagtatttc ctttggtcaa ggtatgagtt tggctcaggc tcttcaagac cgtaaacgcg
    25201 gtgctgcccc aaaatcaatc cagtcaagcg gtcttccatt tggtcaattt acagctggcg
    25261 caaaaccagc atctagcgag gcaaattggt ccattggtga tattgctctc cacaagaaat
    25321 ggggagaggg aaccgttctg gaagtttcag gtagcggtgc taggcaggaa ttgaaaatca
    25381 atttcccaga agtaggtttg aaaaaacttt tagccagtgt ggctccaatt gagaaaaaaa
    25441 tctaattttc catccttctc acgaataata aagtgaggag gatttttatg tacagtattt
    25501 cattccaaga agattcacta ttaccaagag aaaggctggc caaggaagga gttgaagcgc
    25561 ttagtaacca agagttgcta gctattttac tcaggacagg aacacgtcaa gttagcgttt
    25621 ttgaaattgc ccaaaaagtc ttgaacaatc tttcaagcct aacggatttg aaaaaaatga
    25681 ccctgcagga attgcagagt ttgtctggta ttgggcgtgt taaggccata gaattacaag
    25741 ctatgattga actggggcat cgtattcaca aacacgagac tcttgaaatg gaaagtattc
    25801 tcagcagtca aaagttggcc aagaagatgc agcaggaatt aggggataaa aaacaagagc
    25861 acctggtggc actctatctc aatactcaaa atcaaatcat ccatcagcag accattttta
    25921 tcggttctgt aactcgtagt atcgctgaac cgcgagagat tcttcactat gcaatcaagc
    25981 atatggcgac ttctcttatc ttggtccaca atcatccttc aggagcggta gcgcctagcc
    26041 aaaatgatga ttatgtcact aaacttgtta aagaagcctg cgaactgatg ggaattgttc
    26101 tcttggacca tttgattgtc tctcattcta attactttag ttatcgtgaa aagacagatt
    26161 taatctaaag ttcattaacg acatagtcaa agagtttctt atctttggga cgattttcaa
    26221 aaagaagttc tggatgccat tggacaccga gaaaggcgac atcatccgta ctcatgacag
    26281 cctcaatgat accatcttta ggatcatgag ccacaacttt taaatttggt gctaagtcct
    26341 tgatgctctg gtggtggaag gagttgatat gagagatttc tccatagatt tcttggagaa
    26401 cggtatctgg ttctgtcacc aagcgttgag ttgtgtactc aacagaagaa tcctgccaat
    26461 ggtcttcgat atcttggtac aaagttccac ccatggcaac gttaaagagt tgggtaccac
    26521 ggcagacaga gaaaatgggc tttttttgtt taatagcttc cttgatgagg gccagttcga
    26581 agatatctct ttgaaggtga tagtcatcgc tatcaatggt tttgggttcg ccataaaatt
    26641 ttggatcgac attttgccca cctgttaaga tgagcttgtc aatcaaactg atatagtggc
    26701 aggccatttc ttgatcacca atcggtagga tgatgggaat ccctccagca tctttaacgc
    26761 cttcaacaaa gccttttgtt gcgtagctca tcatgatgtc atcatctgga tgagtttttt
    26821 cgtttcctgt aatcccaata actggttttt tcataaaatg attttcgctt tctaatcctc
    26881 ttttcgcatg aagtagagga gggtttggag ttcacttgtc aaatcgacat actgaacgac
    26941 cacgtctttt ggtaaatgca gatggactgg tgaaaaactg agaattcctt tcacaccagc
    27001 atcaaccaag agattagcaa cctcttgtga cttgacgctg ggaacagtta ggatagcagt
    27061 cttcacatca gcatccttga ttttatcctt gatctgagaa atcccgtaaa tgggaatccc
    27121 gtcaggagtt tgggtaccga cttcaggatg gtcgtctagg tcaaaggcca tgataatctt
    27181 catcttgtta cgttcgtgga agcggtagtg gagaagggca tggcccatat ttccaatacc
    27241 aaccagcatg acattggtaa tagagttgtc attgagcaaa tcggcaaaaa atgtcattag
    27301 ttttttgaca tcatagccaa aaccacgacg accaagttca ccaaaatagg aaaaatcacg
    27361 acgtacggtc gctgaatcaa taccgatagc ctctgcaatt tgcttagagt tggcacgttc
    27421 aatcttttct gcatgaaatc tcttaaaaat tcgatagtag agagagagtc tttttgctgt
    27481 agcttttgga atagcaaact gtttatcttt cacaaaatca caacctttct attcttctat
    27541 tttatagaaa cattgtgaaa aaatcaacaa aaataagaaa aaactaagaa aaatcttagt
    27601 tttgatgtaa aaaatctgca tgagatagaa aacggtagag gtctccgacc agcccctgat
    27661 aaactttttt gcccctaaaa gtcagagaag tcacataaag tgtatctggt aaggttacac
    27721 atcctgacaa agtcaacatg agagcctcat gatcctcata cttgagagta cgctctacat
    27781 gatagcagtc cttataggtc agttcaaaca ttttggctct atctttccga ttttgtaaag
    27841 acaccacgtt ctaccaagct atccatgagg aagtagaatt tttcctgatg aatatggtgg
    27901 tcttctgatt tgaaaatatc aactagacga aggccaaact tgtcagtgat attgatttta
    27961 gcccctgtaa gttccttgtt aatgatgatt ttgagttgga agccttcacc gctgtttggc
    28021 actttttcca aaaggcgagt cagttcatag ttaccaacct tagtttcaaa aaaggtgtta
    28081 tctttgaggg tgaatttttt aacagaaggg ctaagagtgt aatcgtaacg acaatttttt
    28141 aactgaatga ttttttcaaa tgccatatgg ctaacctccg ataatttctt ttaaggtttt
    28201 tgcgagggtt tgtaggtctt caacggtatt ttgtggcgac aaactgatgc gaagggattc
    28261 cttcaagcgt tctgaatttg cgccatacat ggcttcaaga acatggctgg attggacaac
    28321 gcctgcagta caggctgagc cagtagagat tgaaattcca gctaaatcta gccgaaggag
    28381 taagaggtca tttttctgac caggaaatcc aatattgaga acataaggga gatgatgttt
    28441 tcctctattc aagtaatact gaatgccctc cagctctgcc agaaaggcag tttctagatt
    28501 ttgtacatgt tgaaaatgtt cttcttgttt ttctaggtct tcttttaggg ctgcaaccat
    28561 gcctacgatg gcaggcagat tttcagttcc tgcacgtttt ttctgttcct ggtctccgcc
    28621 atgtagatag gaatcaaagt ccatgctaga tgcgtagaga aaaccgattc ccttaggacc
    28681 atggaatttg tgggcagaag cagtgagaaa atcaatgccc aattcttctg aatgaattgg
    28741 gattttacca atagcctgaa ctgcatcaac atgataggca gcagggtgtt gcttgagtat
    28801 ttggccaatt tcagcgatgg gcagtaggtt tcctgtctca ttattggcaa acatggtaga
    28861 aaccaaaatc gtatcgtcac gtaaagcctt ttgaatttgc tgggctgtga tttcttgatt
    28921 ttttggctgg ataatggttg cttcaaaccc aaagtgttga accaagtaat caattgtttc
    28981 aaggacagca tggtgctcga tggcagttgt gatgatatgt tttccttgtt cttggtgacg
    29041 aagacagtag ccaatgatgg tagtattatt gccttcagtc ccaccagaag tgaaaaagat
    29101 atgttgaggt tttgtcctta gtaactgggc tagttcctga cgggcttctc gcaagagttt
    29161 gccagcttga cgaccatgac catgaatact agaaggattt ccgtgggttt cttgcataac
    29221 cttggtcata gctgaaatag caactgctga cataggagtc gttgcagcat tgtccaaata
    29281 aatcaaagaa tcaccttatt tctttttatt gtaggcaaag agtgggctga ctggttttct
    29341 ttcgtgaata cggacgatag catcaccaat taactcacta gcagtgatgt agcatacatt
    29401 tttaggagtt ttttcttttg ttgctactga atcagtcaca agaatttctt taatattagt
    29461 attgtcaaga agttcagcag ctccctcgac gaagagaccg tggctagaaa cagcataaat
    29521 ttctgtagct ccttcacgtt caacgatttt agaagcttca gagaaggtac gtcctgtatt
    29581 taaaatatca tcaatcaaga tagctttctt accttcaaca tcaccaataa tataaccttc
    29641 gttacgagtt gcatcgtctt gagggtagtc gataatggcg ataggagcat caagatattc
    29701 agccaggcta cgcgcacgtt tgacacctga atttttaggg ctaacgacaa caacatctga
    29761 accaagcaat cctttatcgc agtaatgttt tgcgaatagg ggaacagtga aaagattatc
    29821 cactggaata tcaaagaaac cttgaacctg aacggcatgc aaatcaagag tcaggatacg
    29881 atcaactcca gccttaacca gcatattggc aactagtttt gctgtaagtg gctcacgagg
    29941 acaagcaatg cggtcttgac gtgcatagcc aaaatatgga aggacaacgt tgatactgtg
    30001 ggcacttgca cgcacacaag catcgaccat gattaacaat tccattaggt ggttgttgac
    30061 agggaaactt gttgattgga tgatgtaaac atcataacca cggacacttt cttcgatatt
    30121 tacttggatt tctccgtctg aaaattgacg tgatgatagt tttccaagtg ggacaccaac
    30181 agcttgggca attttttgtg caatctcttg gttagagttg agtgcgaaaa gtttcatgtt
    30241 ttttctatct gacattatag accgtcctct gtaaacttta taaatcctag ttatatttac
    30301 cttacatata tgaactggga tttgtgtatt tttatctttt ctattttacc aaaaaatgga
    30361 gattatttca gctatttttc atacttttga caaatcgaac caattttgaa ggagcttttt
    30421 ggtaggaaat ctgatttttc tctaaaaatt gtcggaaatc ctgtttgcct tgctcatgat
    30481 tttccacttc aagctccaat tcgtaatctg ttatatcaaa gtatcggctc tgatccagtg
    30541 ccatgagacc aatagctgtt ttcatttcat agcgaagcgt tgttagacaa ccaagaacct
    30601 gccagttctt actttggata ccatgtttcg ccaattcatc cagtactagc ccttgaggaa
    30661 gttcttcctt actcagatag ttctcagcat cttttagttg taatttttgg ttgtattcca
    30721 tgtttccaac actctgcggg actttgagtg tcaactcagc ccagtcttca aaggttcgaa
    30781 tgcgcatagc gactttcttt tctcgcagtt caaaatcagg cgtgtcgatg tagtaatttg
    30841 tttgaagaac aggagtgaca cctgtgaact ggtcttttag acgattgtat tcatcttttt
    30901 tcaatagtgt tttcaattca atttctaaat gtttcatttt tcttaccttt ttttatcgtt
    30961 gaaagcggat ttatggtata ataagcattg tatttattgt atatgaatct ggagaaaaaa
    31021 tcaaagatat ttttgacgga taatatgaga acaagggaga atatatgacc ttagaatggg
    31081 aagaatttct agatccttac attcaagctg ttggtgagtt aaagattaaa cttcgtggta
    31141 ttcgtaagca atatcgtaag caaaataagc attctccaat tgagtttgtg accggtcgag
    31201 ttaagccaat tgagagcatc aaagaaaaaa tggctcgtcg tggcattact tatgcgacct
    31261 tggaacacga tttgcaggat attgctggct tacgtgtgat ggttcagttt gtagatgacg
    31321 tcaaggaagt agtggatatt ttgcacaagc gtcaggatat gcgaatcata caggagcgag
    31381 attacattac tcatagaaaa gcatcaggct atcgttccta tcatgtggta gtagaatata
    31441 cggttgatac catcaatgga gctaagacta ttttggcaga aattcaaatt cgtactttgg
    31501 ccatgaattt ctgggcaacg atagaacatt ctctcaacta caagtaccaa ggggatttcc
    31561 cagatgagat taagaagcga ctggaaatta cagctagaat cgcccatcag ttggatgaag
    31621 aaatgggtga aattcgtgat gatatccaag aagcccaggc actttttgat cctttgagta
    31681 gaaaattaaa tgacggtgta ggaaacagtg acgatacaga tgaagaatac aggtaaacga
    31741 attgatctga tagccaatag aaaaccgcag agtcaaaggg ttttgtatga attgcgagat
    31801 cgtttgaaga gaaatcagtt tatactcaat gataccaatc cggatattgt catttccatt
    31861 ggcggggatg gtatgctctt gtcggccttt cataagtacg aaaatcagct tgacaaggtc
    31921 cgctttatcg gtcttcatac tggacatttg ggcttctata cagattatcg tgattttgag
    31981 ttggacaagc tagtgactaa tttgcagcta gatactgggg caagggtttc ttaccctgtt
    32041 ctgaatgtga aggtctttct tgaaaatggt gaagttaaga ttttcagagc actcaacgaa
    32101 gccagcatcc gcaggtctga tcgaaccatg gtggcagata ttgtaataaa tggtgttccc
    32161 tttgaacgtt ttcgtggaga cgggctaaca gtttcgacac cgactggtag tactgcctat
    32221 aacaagtctc ttggcggtgc tgttttacac cctaccattg aagctttgca attaacggaa
    32281 attgccagcc ttaataatcg tgtctatcga acactgggct cttccattat tgtgcctaag
    32341 aaggataaga ttgaacttat tccaacaaga aacgattatc atactatttc ggttgacaat
    32401 agcgtttatt ctttccgtaa tattgagcgt attgagtatc aaatcgacca tcataagatt
    32461 cactttgtcg cgactcctag ccataccagt ttctggaacc gtgttaagga tgcctttatc
    32521 ggtgaggtgg atgaatgagg tttgaattta tcgcagatga acatgtcaag gttaagacct
    32581 tcttaaaaaa gcacgaggtt tctaagggat tgctggccaa gattaagttt cgaggtggag
    32641 ctattctggt caataatcaa ccgcaaaatg caacgtatct attggacgtt ggagactacg
    32701 ttaccattga cattcccgct gagaaaggct ttgaaacctt ggaggctatt gagcttccat
    32761 tagatattct ctatgaggat gaccactttc tagtcttgaa taaaccctat ggagtggctt
    32821 ctattcctag tgtcaatcac tctaatacca ttgccaattt tatcaagggt tactatgtca
    32881 agcaaaatta tgaaaatcag caggttcaca ttgttaccag actagatagg gacacttctg
    32941 gcttgatgct ctttgccaag cacggttatg cccatgcacg attagacaag cagttgcaga
    33001 agaaatctat cgagaaacgc tactttgctt tggttaaggg agatggacat ttggagccag
    33061 aaggggaaat tattgctccg attgcgcgtg atgaagattc cattattacc agacgagtgg
    33121 ctaaaggcgg aaagtatgcc catacttcat acaagattgt agcttcttat ggaaatattc
    33181 acttggtcta tattcacctg cacactggtc gaacccatca aatccgagtc catttttctc
    33241 atatcggttt tcctttgctg ggagatgatt tgtatggtgg tagtctggaa gatggtattc
    33301 aacgtcaggc tctgcattgc cattacctat ccttttatca tccattttta gagcaagact
    33361 tgcagttaga aagtcccttg ccggatgatt tcagtaacct tattacccag ttatcaacta
    33421 atactctata aaaactgtct cagagtataa ttattatctt aaaggagaaa actcatggaa
    33481 gtttttgaaa gtctcaaagc caaccttgtt ggtaaaaatg ctcgtatcgt tctccctgaa
    33541 ggggaagagc ctcgtattct tcaagcaaca aaacgcttag taaaagaaac agaagtgatt
    33601 cctgttttgc ttggaaatcc tgaaaaaatt aaaatttatc ttgaaattga aggaatcatg
    33661 gatggttatg aggtcatcga ccctcaacat tatcctcaat ttgaagaaat ggtttctgcc
    33721 ttggtggagc gtcgcaaggg caaaatgact gaagaagatg tacgcaaggt tttggttgaa
    33781 gatgtcaact actttggtgt gatgttggtt tacttgggct tggttgatgg aatggtgtca
    33841 ggagcgattc actcaacagc ttcaacagtt cgcccagctc tacaaatcat caaaactcgt
    33901 ccaaatgtaa ctcgtacttc aggagccttc ctcatggttc gtggtacgga acgttaccta
    33961 tttggagact gtgccattaa tatcaatcca gatgcggaag ccttggctga aattgccatc
    34021 aactcagcaa tcacagctaa gatgtttggc atcgaaccta aaattgccat gttgagctat
    34081 tctactaaag gttcagggtt tggtgaaagc gttgataagg tcgttgaagc aactaaaatt
    34141 gctcacgact tgcgtcctga ccttgaaatc gatggtgagt tgcaatttga tgcggccttt
    34201 gttcccgaaa ctgcagctct gaaagctccg ggaagcacag tagctggtca agcaaatgtc
    34261 ttcatcttcc caggtatcga ggcaggaaat atcggttaca agatggctga acgcctgggt
    34321 ggctttgcgg ctgtaggacc tgttttgcaa ggtttaaaca agccagttaa tgatctttct
    34381 cgtggatgta atgcagatga tgtttacaag ttgaccctca tcacagcagc tcaagcagtt
    34441 catcaatagt gaaaactata aagtgatata ctatgctata ctgtagttat gaaactatgt
    34501 acgaaaagca ctgccattaa ttcctgagaa ctaaattact gattggtgtc aaaaaggaaa
    34561 acttccaagc gatgatatcc tgtctataca cgacctatag aaatctgtaa tatacatgtc
    34621 cgtaaaacga taaattccct ttttgatttt aaatgagtat gaaaagagaa ttttttggct
    34681 ctttgtcaac tgtagtgggt tgaagtcagc taagctcgag aaaggacaaa ttttgtcctt
    34741 tcttttttga tattcagagc gataaaaatc cgttttttga agttttcaaa gtttcgaaaa
    34801 ccaaaggcat tgcgtttgat aagtttgatg agattattgg tcgcttccaa tttggcgttt
    34861 gaatagggta gttgaagggc gttgacgatt ttctctttgt cctttagaaa ggttttaaag
    34921 acagtctgaa aaagaggatg aacctgcttt agattgtcct caataagccc gaaaaatttc
    34981 tctggttcct tattctggaa gtgaaacagc aagagctgat agtggtgttt caagtcttgt
    35041 gaatagctca aaagcttgtt taaaatctct ttattggtta agtgcatacg aaaagtagga
    35101 cgataaaaac gtttatcact cagtttacgg ctatcctgtt gaatgagttt ccagtagcgc
    35161 ttgatagcct tgtattcatg ggattttcga tgaaactgat tcatgatttg gacatgcaca
    35221 cgactcatag cacggctaag atgttgtaca atgtgaaagc gatcaagaac gattttagca
    35281 ttcgggagtg aaacagtctg ggagactgtt tcagcctgag cctagaaatt tgaaagcgaa
    35341 gctgtttagc caagccatag taagggctaa acatatccat agtaatgatt ttgacgcgac
    35401 atcggacggc tctatcgtag cgaagaaagt gatttcgaat gatagcttgt gttcttccct
    35461 caagaacagt gatgatattg agcttgtcaa aatcttgcgc aataaagctc atctccatct
    35521 cccgattgaa acagtcactc cccggactgt ttcaacgtcc caagacataa tctcaggcag
    35581 atttctaaaa ttacactcaa agtggaagtc attgagctta cgaataacag ttgaagttga
    35641 gatgaaaagc tgatgggcaa tatcagtcat agaaatcttt tcaattaact tttgagcaat
    35701 cttttggttg atgatacgag ggatttggtg atttttcttg acgatagaag tttcagcgac
    35761 catcattttt gaacagtgat agcacttgaa tcgacgcttt ctaaggagaa ttctagtagg
    35821 cataccagtc gtttcaagat aaggaatttt agaaggtttt tgaaagtcat att
//
LOCUS       DAVKLW010000016        34045 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKLW010000016 DAVKLW010000000
VERSION     DAVKLW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.016"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..194
                     /locus_tag="VW324_000773"
     CDS             <1..194
                     /locus_tag="VW324_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEU9726444.1"
                     /translation="AMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDV
                     EDVLQMPLLGVVPDFDKMK"
     gene            204..893
                     /locus_tag="VW324_000774"
     CDS             204..893
                     /locus_tag="VW324_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9726445.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTAPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            908..2269
                     /locus_tag="VW324_000775"
     CDS             908..2269
                     /locus_tag="VW324_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9726446.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRVIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            2275..3018
                     /locus_tag="VW324_000776"
     CDS             2275..3018
                     /locus_tag="VW324_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU9726447.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            3015..3839
                     /locus_tag="VW324_000777"
     CDS             3015..3839
                     /locus_tag="VW324_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU9726448.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYGWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            3841..4719
                     /locus_tag="VW324_000778"
     CDS             3841..4719
                     /locus_tag="VW324_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9726449.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            4720..6054
                     /locus_tag="VW324_000779"
     CDS             4720..6054
                     /locus_tag="VW324_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU9726450.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPILNTKIKVTDRLIDIQFWKIAL
                     TIIVDLIILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            6082..7506
                     /locus_tag="VW324_000780"
     CDS             6082..7506
                     /locus_tag="VW324_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726451.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            7571..8659
                     /gene="wecB"
                     /locus_tag="VW324_000781"
     CDS             7571..8659
                     /gene="wecB"
                     /locus_tag="VW324_000781"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU9726452.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            8822..9691
                     /gene="rfbA"
                     /locus_tag="VW324_000782"
     CDS             8822..9691
                     /gene="rfbA"
                     /locus_tag="VW324_000782"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU9726453.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            9692..10285
                     /locus_tag="VW324_000783"
     CDS             9692..10285
                     /locus_tag="VW324_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU9726454.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            10297..11343
                     /gene="rfbB"
                     /locus_tag="VW324_000784"
     CDS             10297..11343
                     /gene="rfbB"
                     /locus_tag="VW324_000784"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU9726455.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFPDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGLGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKETIKWYTDNQDWWKSEKE
                     AVEANYAKTQQVIK"
     gene            complement(11405..12259)
                     /gene="rfbD"
                     /locus_tag="VW324_000785"
     CDS             complement(11405..12259)
                     /gene="rfbD"
                     /locus_tag="VW324_000785"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU9726456.1"
                     /translation="MILITGANGQLGTELRYLLDERNVDYVAVDVTEMDITNSEMVEK
                     VFAEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKYGAILVYISTD
                     YVFDGKKPVGQEWEVDDIPDPQTEYGRTKRMGEELVENLTSQHYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYVTENQKEYGYYHLSNDAAEDT
                     TWYDFASEILKDSDVEVVPVDSSKFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALKE
                     FYKQEVRK"
     gene            12505..14487
                     /locus_tag="VW324_000786"
     CDS             12505..14487
                     /locus_tag="VW324_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9726457.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKAEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            14787..20090
                     /locus_tag="VW324_000787"
     CDS             14787..20090
                     /locus_tag="VW324_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9726458.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSQVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGSKTEEELLALEKEKPAE
                     EKPKEDKPAVAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGNYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMEALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSIVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTSLFLASVSLALSALFVVKTKKD"
     gene            complement(20257..22416)
                     /locus_tag="VW324_000788"
     CDS             complement(20257..22416)
                     /locus_tag="VW324_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU9726459.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(22413..23009)
                     /gene="recU"
                     /locus_tag="VW324_000789"
     CDS             complement(22413..23009)
                     /gene="recU"
                     /locus_tag="VW324_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9726460.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            23075..23602
                     /locus_tag="VW324_000790"
     CDS             23075..23602
                     /locus_tag="VW324_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9726461.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFENHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            23672..24001
                     /gene="gpsB"
                     /locus_tag="VW324_000791"
     CDS             23672..24001
                     /gene="gpsB"
                     /locus_tag="VW324_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9726462.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            24022..24402
                     /gene="rnpB"
                     /locus_tag="VW324_000792"
     ncRNA           24022..24402
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW324_000792"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            24487..25644
                     /locus_tag="VW324_000793"
     CDS             24487..25644
                     /locus_tag="VW324_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9726463.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDARVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            25657..27051
                     /gene="mapZ"
                     /locus_tag="VW324_000794"
     CDS             25657..27051
                     /gene="mapZ"
                     /locus_tag="VW324_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9726464.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLEDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPQPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            27127..28551
                     /gene="gndA"
                     /locus_tag="VW324_000795"
     CDS             27127..28551
                     /gene="gndA"
                     /locus_tag="VW324_000795"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9726465.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELVNSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            28563..29252
                     /locus_tag="VW324_000796"
     CDS             28563..29252
                     /locus_tag="VW324_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9726466.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            29350..30378
                     /gene="cbpC"
                     /locus_tag="VW324_000797"
     CDS             29350..30378
                     /gene="cbpC"
                     /locus_tag="VW324_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9726467.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQE
                     KWYYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWI
                     YEEGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGK
                     MLTDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRS
                     SGAMATGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYN
                     FASSGEWI"
     gene            30396..31394
                     /gene="cbpJ"
                     /locus_tag="VW324_000798"
     CDS             30396..31394
                     /gene="cbpJ"
                     /locus_tag="VW324_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9726468.1"
                     /translation="MKILKKMMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWRTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(31512..32744)
                     /locus_tag="VW324_000799"
     CDS             complement(31512..32744)
                     /locus_tag="VW324_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9726469.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(32755..33375)
                     /locus_tag="VW324_000800"
     CDS             complement(32755..33375)
                     /locus_tag="VW324_000800"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726470.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKVDLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            complement(33275..33613)
                     /locus_tag="VW324_000801"
     CDS             complement(33275..33613)
                     /locus_tag="VW324_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HEU9726471.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
BASE COUNT        10531 a         5945 c         7448 g        10121 t
ORIGIN      
        1 cggctatgac tccctcttct ccaaatgttc gacgcaacac cttagttggt tttcttggag
       61 gagccgccgt aacagtaatt actgttcttt tgattgagtt gctcgatacc cgtgtaaaac
      121 gtcctgaaga tgttgaagat gtactgcaaa tgccacttct aggagtcgtt ccagattttg
      181 ataaaatgaa ataggaggaa gttatgccaa cgttagaaat ctcacaggcg aaattagaac
      241 ttgtgaaaaa ggcagaggaa tattataatg ctttgtgcac gaacctacag ttaagtggag
      301 atgatttgaa agtattttct atcacttctg tgaaagcagg agaaggaaaa acaacgactt
      361 ccaccaatat cgcttgggct tttgcgcgtg caggttacaa aacgcttctg attgatgcag
      421 acatgcgtaa ctccgttatg tcaggtgtct ttaaatcaag ggaaaggatt actggactaa
      481 cagaatttct atcagggaca acagacctgt cacaggggct ttgcgatacc aatattgaga
      541 atctctttgt gattcaggct ggctctgtgt caccaaatcc gacagccctt cttcaaagta
      601 agaatttcag tacaatgctt gaaaccttgc gtaaatattt tgactatatc gtcgtagaca
      661 ctgcccctgt cggtgtcgtg attgatgcgg ctatcattat gcagaaatgt gatgcttcta
      721 ttttagtgac gaaggcaggt gaaacaaaac gacgggatct tcaaaaagcg aaagaacagt
      781 tggaacaaac tgggaagtcg tgtttaggag ttgtgttgaa taaactcgat acttcagtag
      841 agaaatacgg ttcttatggg agttatggga attacaggaa acagaaaaaa taggttgagg
      901 gatagaaatg agtgaaaaac tagcaaagcc ttcagtagcc atagtccaga gttttcttgt
      961 tattttattg acttatctac ttagcgctgt gagagaaaca gagattgttt caacaacagc
     1021 tattgtactt tatatcctcc actattttgt cttttatatc agtgattatg gacggaattt
     1081 ctttaaaagg ggatatttga ttgaacttgt ccagacattg aaatatatcc tattctttgc
     1141 gctagcgatt agtatttcta attttttctt agaagatcga tttagtattt ccagaagagg
     1201 catgatttac ttcctcttat tacatgtttt cttagtctat gtgctaaacc catttatcaa
     1261 gtggtattgg aagcgggctt atcccaactt taaaggaagt aagaagattc tcctacttac
     1321 agcaacttct cgtgttgaaa aggtactgga tagactaata gaatcagatg atgttgttgg
     1381 ggagttggta gccgtcagtg ttttagataa accagatttt cagcatgatg atttaaaggt
     1441 tgtagcagag ggggagatag taaattttgc gactcatgag gtggtcgatg aagtctttat
     1501 caatcttcca agtgaaaaat acaatattgg agagcttgtc tctcagtttg aaacgatggg
     1561 aattgatgta acagtcaatc taaatgcttt cgattgggca cgtaacaagc aaatttgtga
     1621 gatggcagga ctaaatgttg tgactttttc tacaacattt tataagacta gccatgtgat
     1681 tgctaagcgg gttattgata ttgtcggttc cctggtaggt ttgatattat gtggtctagt
     1741 cagtattgtg ctggttcctt tgattcgaaa ggatgggggt tctgcttttt ttgctcagac
     1801 gcgtatagga aaaaatggcc gccattttac tttttataag tttcgctcta tgtgtgtgga
     1861 tgctgaggag aaaaaaagag aactcatgga acaaaatacc atgcagggtg gaatgtttaa
     1921 ggtggacgat gatcctcgta tcacgaaaat tggtcatttt atacggaaga cgagcttgga
     1981 cgagctacca cagttttata atgttctaaa gggagatatg agtttggtag gtacacgacc
     2041 accaacagtg gacgagtatg agcactatac cccagaacaa aaacgtcgcc taagttttaa
     2101 acctggcata acaggtttat ggcaggtcag cggacgaagt gaaatcaaaa atttcgatga
     2161 agttgtcaaa ttagatgtgg cctatattga tggttggaca atctggaaag atattgaaat
     2221 tttattgaag acagttaagg ttgtattgat gaaggatgga gcgaaatagt tcatatgaat
     2281 gagagaattc aaatattagg aattactata gatccattaa cgatgaaaga aacagtggat
     2341 gctgttgaac agtatgtttt aaagaagcac cctttgcact tgatgggagt taacgcggat
     2401 aaaattaatc agtgctatga agatgagaaa atcaaaaaaa tcgttaatga atcaggaata
     2461 attaatgccg acggtgcttc agtagtttta gcgagtaagt ttttaggaac accagttcct
     2521 gagcgagttg ctggaataga cttgatgaaa catttactag agttgtctaa tgaaaaagga
     2581 tactcggtct acttttttgg agcaaaagaa gacgttttac aagatatgtt aaaaattttt
     2641 gaagaaagat atccagctct aaatgttgtt ggctatagaa atggatattt ttcgcctgaa
     2701 gatgaaaaac atattcagga agatattaaa gagaaaaaac cagattttgt gtttgtagga
     2761 attacttctc ctaagaaaga atatattatc caatcattca tggataatgg aattaatgct
     2821 gtgtttatgg gggttggtgg aagttttgat gtactatcag ggcatatcaa acgagctcca
     2881 ttatggatgc aaaatgctca cctagagtgg ttgtttcgtg tggcaaatga gcctaaacgt
     2941 ctctttaagc gttattttgt agggaatgtt tcatttataa gaaaagtttt aagagcgaaa
     3001 aggggatata aactttgaac caaacagaaa tagttaaatt gattcagaaa gttgaattgg
     3061 atgctataaa agagtttcaa aaaatttgta aagagaacaa tatagatttc ttccttcgtg
     3121 gggggagtgt acttggggca gtcaaatatg atggctttat tccatgggat gatgacatgg
     3181 atattgctgt gcctcgtgaa ggctacgata aacttccagg tatctttaag gatagaatta
     3241 tcgcaggcaa atatcaggtt cttgcttatc aatattgcga tactttacat tgctactttc
     3301 ctcgcttatt ccttttagaa gatgaaagaa aacgtttggg attgccacga aataccaatt
     3361 taggtttgca tttgattgat atcattcctt tggatggtgc cccaaatcat tcatttttaa
     3421 gaaagcttta cttcggtaaa gtatactggt atcgtttttt agcaagttta ggaactacct
     3481 atgttggaga ccatgtggat atgcattccg ctaagcaaaa actaatcatt ggtttcttta
     3541 aaaagctagg atttgcaaaa ctattccctc aaaattatgt gtacagacgc ttggataatc
     3601 tctataaaaa gtatggttgg aaaaagcaga agtatgctgg gacaattaat gcatctctgt
     3661 ttgccaaaga agtcatgcca gtagagattt ggggagaagg agtagagaag ccttttgagg
     3721 atgccttctt taaagttccc acggagtatg accgctatct gaaaagactt tacggagaaa
     3781 actatcttca cgaagagcct agtgatgatg aaaagaaatc gcatttagga ggacaataat
     3841 ttgttttgtt atattatttt gcattacaaa gtcttagagg aaactatttc ttgtgttaaa
     3901 tcaataaaag aaggcaattc caatacaaag cagatcgtta ttattgataa tttttctaat
     3961 aacggtacgg gtgaaaaact acaagagctg tatgagtcag attcagagat tgatgtcttg
     4021 attaaccatg aaaatgctgg tttcgctcga ggtaataatg tagcatatca gtttgctaag
     4081 gaaaagtaca aacctgattt tatggttatc atgaataatg atattgagat agaaacagaa
     4141 gagtttgaaa aaatcgtgac agatatctat cggaaggaaa aattccattt gttaggacca
     4201 gatatctttt cgacgacgta tcagcttcac caaaacccaa aacggttgac gcattatact
     4261 tatgaagagg ttaaggctct caatgaaaaa tttaagaaag ggagccaagt tagtctagca
     4321 ttaaaaatta aatgttggtt gaagtctagt aaagttcttc ggacagcaat ctatcaaaat
     4381 aggcgtaaaa agaaatcagt agactataga aaacaggtag aaaacccaat tcttcatggt
     4441 tcgtttattg tatattctag agattttatt gagaaagagg agtatgcttt taatcccaat
     4501 accttcttct attatgaaac agagatatta gattatgaag ctgagttaaa aggatataag
     4561 agaatttata caccgaagat taaggtcttg caccatcaaa atgttgcaac caatcaagtc
     4621 tatacaaact tagtagaaaa aaccttgttt tcaaacaaat gcaactttga atccactagt
     4681 tattttttga agttgatgaa agaaaacgag ggtgtgtaaa tgacttattt atttttactc
     4741 tgcctgacct tattcttatt aactttcttc tatttttttg ctttcaatca agatttgata
     4801 gctccgccag ttgtcatgtc tgttatgttt ctcattagtt ctgtatttgc ccttattaat
     4861 gtgcaaaact ggaatattga gtatagtggt ttagcttatc ttttgattat tagtggcatt
     4921 attgtttttt caatgcctct cttggctctt aattcgccta ttctaaatac caagattaaa
     4981 gtgacggatc ggctcattga tatccaattc tggaaaatag ctcttactat tatagttgac
     5041 ctcattattc tatatcttta taggagagag attcataatc ttgcacttag ccatggttat
     5101 acgggttcaa attttcagtg gttctttaga aatgctacca gttatgaagg tgagctaaca
     5161 gtgcgaactt cgattcgggt cctcattcgt atcattgacg tatctgctta tatttttgga
     5221 tatactttta ttaataattt cttcatttat agtcataaac gctctaaaga tttactgctc
     5281 ttagttccat tcttgatttt tatttctaaa accttattat ctgggggtag attggatatt
     5341 ataaaaattt taattgcgta tgttgtaatg gcctatattc agcaaaaacg aaaagttggc
     5401 tgggataagg tcatctccca taaatatatg agacttggtt ttgtaggctt gatagctggg
     5461 attcctacct tttactattc tttattctta tctggacgtt ctacgactag aactgtattt
     5521 gaaagtattt caacctattt aggaggttcg attcagcatt ttaatcagta tattcaaaat
     5581 cctattggag tagctgaggt ttttggcgat gagtcatttg tagctattat gaatattttg
     5641 ggtaatcttg gctttgtcaa ttacaatagt accgttcact tagaatttcg gcagttaggg
     5701 attactatgg gtaacgttta tactttcttt agaagaccct ggcatgactt tgggttagtc
     5761 ggtatgtata tcttttcctt tgttgtcggt gtgttttttg ctatttttta tttgaaatta
     5821 agaaagagcc gtgctggttt taaattggat attcatacaa ttatttactc ttatttcttt
     5881 tattggattt ttttatcgtc cattgagcaa tactcgttta caacaatcag tttgtttaca
     5941 cttgtattta tagtcttggt ttacctaatg gctttcttct attggaattt ggatttccac
     6001 agaggaaaac tagtaattaa actctctgac acaagtatta aatcggaaaa ctgaaaacaa
     6061 taaaaataga gaggatagtt gatgaatagc aaaattaaaa atatactaac taatttctct
     6121 tatgtcattt cttcaaatct tttgacagtc ttgacctctt ccttggttgt cttaattttc
     6181 cccaaattaa tgggggtaac agagtacagt tactggcaac tttatatttt ttatctgacc
     6241 tatatcggtt ttttccactt gggttggatt gatgggattt atcttaaata tggtggctta
     6301 gaatataata atttagatag aaaacagttt tattctcaga tgattctatt ttctagcttc
     6361 ttaatgatta tctccttgct attgtttggt ttaaatgtat tagctgtaac agattcgaat
     6421 gcacgatata tttataatat gactattata agtatgataa tcacaaattt aagaacgctc
     6481 tacgtgtatg ttttgcagat gacaaaccgc ttgaaggata gttccatcat tctactcagt
     6541 gatcgcgttt tatatgtgct ccttttattc ctgtttattg tatttggttg gcatgaatac
     6601 aaggttatga ttttggcaga tattctaggt cgatcatttt ctctcatact ttccttctgg
     6661 atttgtaaag atattgtgtt tcagcctttg tcaaaattca tctttaatat aaaagagtct
     6721 tttgataata tccgagttgg tatcaattta atgttatcca acatcgcaag tagtttgatt
     6781 atcggtattg ttcgtctagg aatccaatgg aactggaata tcgaaacatt cgggaaagta
     6841 tctctgacct tgagtgtttc caatctattg atgactttca ttaatgccat tggactagtc
     6901 gtctttccgc tattaagacg aacaaaagca gaaaacttac ctaagattta ttctaattta
     6961 agaaacgttt tgatgcttat catgttcgcg attttgctca tttactatcc tttaaaaatt
     7021 gtattagacc tctggttgcc agcctatcaa gatgccttga ttttcatgac ccttattttc
     7081 cctatgtcag tctatgaagg gaaaatggca ttggtcatta atacttactt aaaggcatta
     7141 agaatggaaa gagatatcct tagagtcaat gccttagtaa tgttgatcag tatgggagtg
     7201 accctggtta caacatacct gttaaatagt ttggagctga ctgttgtatc gatagttgtt
     7261 ttgctagctt tgagaagtat catagccgaa ttaattctgt ctaaaaaact ggatgtttcg
     7321 gttaagaagg atattgtatt agaatttctt ttgacgcttg tctttatttc ttcaagttgg
     7381 tatttgccga ttgggctggc agtaattgtc tacacaatag cctacggttt atatctctat
     7441 ttgaaacacg aagatatcaa aacctattta gcttacttta aagctagtaa aaaaacatca
     7501 aattaaaaaa ttatataatg attaagtggt agattctatt tctaccgttt tagatattcg
     7561 ggaggtaatg atgaaaaaaa ttatgttagt attcggtaca cgtccggaag ccatcaaaat
     7621 gtgtccatta gtcaatgagt tgaaaaaaca cgaagatatg gaaacaattg tgtgtgttac
     7681 tggacaacac aaagagatgg ttagtcctgt tttagattta tttggtgttg taccagatta
     7741 tgatttagaa attatgaagg ctaaccaaac cttgttctct atcacaacta gtatcttgga
     7801 aaagataaaa ccagttttag agaaggaaca accagatatt gtcctagttc acggtgacac
     7861 tacgacaact tatgcagcag ccttggcagc attctatttg ggaattaaag taggacatgt
     7921 tgaagctggt ttgcgaacgt acaatttaca aagtccattt cctgaagaat ttaacaggca
     7981 atcgacatca atcattgcaa cttaccattt tgctccaact gagttggcta aagaaaatct
     8041 cttaaaagaa ggtagagaga atgtttatgt gactggaaat actgtcattg atgctcttac
     8101 aactactgtt caagaggatt atacacacac tcatttagat ttaaacgcta acaatcgtct
     8161 catcttattg actgctcata gacgcgaaaa tctcggcgaa ccgatgagac atatgtttag
     8221 agcagttaaa cgagtattga atgaatacga agatgttaaa gtcatttatc caattcataa
     8281 gaatcctttg gtacgtgaaa cagctgcaga gatctttgga gatacagaac ggattcaaat
     8341 tattgaacct ttggatgttc ttgatttcca caacttcatg aaccaaagtt atatgatttt
     8401 aacagattct ggcggagttc aggaagaagc accttcttta ggaaaacctg tattggttat
     8461 gcgcgatact actgaaagac ctgaaggagt agcggcagga accttgaaat tagtaggaac
     8521 tgacgaagaa acaatttatc aaaacttcaa actacttcta gatgattctg gagaatacaa
     8581 aaaaatgagt caggctagca atccatacgg taatggtgat gctagtcaac agattgttca
     8641 gattttgcgt gggatttaag aagattctct aaaagagtct aagaggaatc cactccacaa
     8701 acataaaact cttatgctat catttatgat tgagatagac tcataactta atatttttat
     8761 cctaagaaaa ttcgttcata ttcaatatga atgaaaaaca acgaagaaag gttatttcat
     8821 tatgaaaggt attattcttg caggcggctc aggtacccgc ctgtacccac ttactcgggc
     8881 tgcgtcaaaa cagctgatgc cggtttatga taaacctatg atttattatc cgttgtcgac
     8941 attaatgttg gctggaatta aagatatttt gattatctca actcctcaag atttgccccg
     9001 ttttaaggac ttgctcttgg atggttccga atttgggatc aagctttcct atgcggaaca
     9061 acctagtccc gatggacttg ctcaggcttt tcttatcggt gaagaattta tcggtgacga
     9121 tagcgttgcc ttgattttag gcgacaatat ctaccatggt cctggtctga gcacaatgct
     9181 tcaaaaagca gccaagaaag agaaaggtgc gactgttttt ggctaccaag tgaaggatcc
     9241 agagcgtttt ggtgtggttg agtttgatac agacatgaat gctatttcta tagaagaaaa
     9301 accggagtat cctcgctcca actatgcagt gacaggactg tatttctatg ataatgatgt
     9361 tgtggagatt gctaaacaga tcaaacctag tgctcgtggc gagttagaaa ttacagacgt
     9421 taacaaggct tacctaaatc gtggtgacct ttctgttgag ctgatggggc gtggttttgc
     9481 ctggttggat acgggaaccc atgaaagctt gctagaagct tctcagtata ttgaaacggt
     9541 tcagcgtatg cagaacgttc aagtggcaaa tctagaagaa atttcctatc gtatgggcta
     9601 cattagtcgt gaagacgtgc tggaattggc tcagcctctt aagaaaaatg aatacggaca
     9661 atatttgctc cgtttgattg gagaagcata gatgtcagat aatttttttg gaaagacact
     9721 tgtggtacgc aagattgatg ctataccagg actgctagag tttgacattc ccgttcatgg
     9781 agacaatcgt ggttggttta aggaaaattt ccagaaggaa aagatgctac cgcttggttt
     9841 tcctgaaagc ttctttgctg cagggaaact gcaaaataac gtcagctttt ctcgcaaaaa
     9901 tgttcttcga ggactccatg ctgaaccttg ggacaagtat atctctgttg cagacgatgg
     9961 gaaggtttta ggatcttggg tagatttgcg tgaaggcgag acctttggaa atgtttacca
    10021 gacagagatt gatgctagca agggaatttt tgtccctcgt ggcgtagcca acggttttca
    10081 agtcctctca gatacagttt cctatagcta tctggtcaat gactactggg ctcttgaact
    10141 caaacccaaa tatgcctttg ttaactatgc tgacccagca ttgggaattg agtgggaaaa
    10201 cctagcggaa gctgaggttt cagaggcaga caaacaccat cctctattaa gggatgtcaa
    10261 accacttacg aaagacgagt tgtaaaagga aacattatga ctgaatacaa aaaaattatc
    10321 gtgacaggtg gagctggttt tatcggttct aactttgtcc actatgttta caataacttt
    10381 ccagatgtcc atgtgacagt gctggacaag ctgacttatg caggtaatcg tgccaatatc
    10441 gaggaaattt taggcgaccg tgttgagttg gttgttggag atattgctga tgcagccttg
    10501 gtagacaagt tggcggctga agcggatgct atcgttcact atgcggcaga aagccacaat
    10561 gataactcgc tcaatgaccc gagtccattt atccacacca actttatcgg aacttacaca
    10621 cttttagaag cggctcgtaa atacgacatt cgtttccacc atgtatcgac tgatgaagtc
    10681 tatggtgacc tgcctctgcg tgaagatttg ccaggtcatg gggaaggcct aggtgagaaa
    10741 tttaccgctg aaaccaagta caatccaagc tcgccttact catcaaccaa ggctgcttca
    10801 gacttgatcg ttaaagcttg ggtgcgctca tttggtgtta aagcaacgat ttctaactgt
    10861 tcaaacaact atggtccata ccagcatatc gagaagttca ttccgcgcca gattacgaat
    10921 atcttgagcg gtatcaagcc aaaactctac ggagaaggta agaatgtgcg tgactggatt
    10981 cacaccaatg accattcatc aggcgtttgg acgattctga ccaagggtca aatcggtgaa
    11041 acttacttga tcggtgctga cggtgagaag aacaacaagg aagtgttaga gctcattctc
    11101 aaggagatgg ggcaacccgc tgacgcttat gaccatgtga ccgatcgagc tggtcacgac
    11161 ttgcgttatg cgattgatgc tagcaagctc cgtgatgagt tggggtggaa gccagagttt
    11221 accaactttg aagcaggcct caaagagacc atcaagtggt acacagataa ccaagactgg
    11281 tggaaatctg aaaaagaagc agtagaggct aactatgcta aaacgcaaca agtgattaaa
    11341 taataagata ctaaaaaagc aagagacctc gaggtctctt gcttttctag tatgtagtaa
    11401 gcttttattt tcttacttct tgtttataaa actctttaag ggcatcttgc caggttggga
    11461 tgacgaaacc tgttgccttt gcctttgcca aactcatagt tgagttgaga ggtcgtttag
    11521 ccttagcggg gaacttgcta gagtctactg gcactacttc aacatcactg tccttaagaa
    11581 tctcgctagc aaagtcatac caagtggtgt cttcagctgc gtcatttgat aagtgatagt
    11641 agccatactc tttttgattt tcagtcacat aggtcatgaa ttcggccaag gtccgtgtcc
    11701 aagttgggcg accgtattgg tcgttgacca ctgtgagcgt tttatgggtt ttggctaggc
    11761 tttgcatggt aaagacaaag ttttttccat aatttccaaa aacccaagca gtacgaatga
    11821 tgtaatgctg tgacgtaagg ttttcaacaa gttcttctcc cattcgcttg gtacgtccgt
    11881 actctgtttg cggatcaggt atgtcatcga cttcccactc ttgtcctact ggtttctttc
    11941 catcaaagac gtagtctgtt gagatataga ccagaatagc tccgtatttc tcagaggcct
    12001 tggctacatt ttcagttcca gttacgttga tggcaaaatc cagttctttc ccttcatctt
    12061 cggctgcatc aacagcagtg taggctgcac aatgatagac tagagttggc ttaacctcag
    12121 caaagacttt ttcaaccatt tcagaattag tgatatccat ttcggtcaca tccactgcaa
    12181 catagtccac atttcgctca tctagtaaat aacgtagttc ggtaccaagt tgaccatttg
    12241 cacctgtaat taagatcata tttttctcct tgatagttct tatacttaat tatatcaaaa
    12301 atatagaaaa atcggttttt agtgaaccct tttttggtat aaactttgtt agtgaagcgc
    12361 aagagagccg aatagttatt actttagaat atccttaaaa gtatttttca gaatttacca
    12421 aattaattaa aaaattcaga aaattatatt gacatctctc tgaaaagagt ctataataga
    12481 gagaaagttt taaaggagaa gatgatgaaa agttcaaaac tatttgccct tgcgggtgta
    12541 accttattgg cggcgactac tttagctgca tgctctggat caggttcaag cactaaagct
    12601 gagaagacat tctcatacat ttatgagaca gaccctgata acctcaacta tttgacaact
    12661 gctaaggctg cgacagcaaa tattaccagt aacgtggttg atggtttgct agaaaatgat
    12721 cgctacggga actttgtgcc gtctatggct gaggattggt ctgtatccaa ggatggattg
    12781 acttacactt atactatccg taaggatgca aaatggtata cttctgaagg tgaagaatac
    12841 gcggcagtca aagctcaaga ctttgtaaca ggattaaaat atgctgctga taaaaaatca
    12901 gatgctcttt accttgttca agaatcaatc aaagggttgg atgcctatgt aaaaggggaa
    12961 atcaaagatt tctcacaagt aggaattaag gctctggatg aacagacagt tcagtacact
    13021 ttgaacaaac cagaaagctt ctggaattct aagacaacca tgggtgtgct tgcgccagtt
    13081 aatgaagagt ttttgaattc aaaaggagat gattttgcca aagctacgga tccaagtagt
    13141 ctcttgtata acggtcctta tttgttgaaa tccattgtga ccaaatcctc tgttgaattt
    13201 gcgaaaaatc cgaactactg ggataaggac aatgtgcatg ttgacaaagt taaattgtca
    13261 ttctgggatg gtcaagatac cagcaaacct gcagaaaact ttaaagatgg tagccttaca
    13321 gcagctcgtc tctatccaac aagtgcaagt ttcgcagaac ttgagaagag tatgaaggac
    13381 aatattgtct atactcaaca agactctatt acgtatctag ttggtacaaa tattgaccgt
    13441 cagtcctata aatacacatc taagaccagc gacgaacaaa aggcatcgac taaaaaggct
    13501 ctcttaaaca aggatttccg tcaggctatt gcctttggat ttgaccgtac agcctatgcc
    13561 tctcagttga atggacaaac tggagcaagt aaaatcttgc gtaatctctt tgtgccacca
    13621 acatttgttc aagcagatgg taaaaacttt ggcgatatgg tcaaagagaa attggtcact
    13681 tatggggatg aatggaagga tgttaatctt gcagattctc aggatggtct ttacaatcca
    13741 gaaaaagcca aggctgaatt tgctaaagct aaatcagcct tacaagcaga aggtgtgact
    13801 ttcccaattc atttggatat gccagttgac cagacagcaa ctacaaaagt tcagcgcgtc
    13861 caatctatga aacaatcctt ggaagcaact ttaggagctg ataatgtcat tattgatatc
    13921 caacaactac aaaaagacga agtaaacaat attacatatt ttgctgaaaa tgctgctggc
    13981 gaagactggg atttatcaga taatgtcggt tggggtccag actttgccga tccatcaacc
    14041 taccttgata tcatcaaacc atctgtagga gaaagtacta aaacatattt agggtttgac
    14101 tcaggggaag ataatgtagc tgctaaaaaa gtaggtctat atgactacga aaaattggtt
    14161 actgaggctg gtgatgaggc tacagatgtt gctaaacgct atgataaata cgctgcagcc
    14221 caagcttggt tgacagatag tgctttgatt attccaacta catctcgtac agggcgtcca
    14281 atcttgtcta agatggtacc atttacaata ccatttgcat tgtcaggaaa taaaggtaca
    14341 agtgaaccag tcttatataa atacttggaa cttcaagaca aggcagtcac tgtagatgaa
    14401 taccaaaaag ctcaggaaaa atggatgaaa gaaaaagaag agtctaataa aaaggctcaa
    14461 gaagatctcg caaaacatgt gaaataactg ttgcaaaata taagaaagga tttagtattt
    14521 ctcttgaatg ctgaatcctt ttttacattt gtaaagaaag attctaaatg tactgacccc
    14581 caaaagttgg agcctctttt tgtcagaata gagaaaattt ttgttaattt tacttgtttc
    14641 ctattgcttt ctcagttatt atttgttata ttaaaagtat aattattttt atttatcagg
    14701 gttaagcatt gcactttcag aggaaggagt attttttaaa aagaaaatgt aaacgcttgc
    14761 tcaaaaatga aaggatttag aagtttatga ataaaggatt atttgaaaaa cgttgtaaat
    14821 atagtattcg gaaattttca ttaggtgttg cttctgttat gattggagct gcattctttg
    14881 ggacaagtca ggttcttgca gatagcgtgc agtctggttc cacggcgaac ttaccagctg
    14941 atttagctac tgctcttgca acagcaaaag agaatgatgg gcgtgatttt gaagcgccta
    15001 aggtgggaga agaccaaggt tctccagaag ttacagatgg atctaagaca gaagaagaac
    15061 tattagcact tgaaaaagaa aaaccggctg aagaaaaacc aaaagaggat aaacctgcag
    15121 ttgctaaacc tgaaacgcct aagacggtaa cccctgaatg gcaaacggta gagaaaaaag
    15181 aacaacaggg aacagtcact atccgagaag aaaaaggtgt ccgctacaac caactatcct
    15241 caactgctca aaatgataac gcaggtaaac cagccctgtt tgaaaagaag ggcttgaccg
    15301 ttgatgccaa tggaaatgca actgttgatt taaccttcaa agatgattct gaaaagggca
    15361 aatcacgctt tggtgtcttc ttgaaattta aagataccaa taataatgtt tttgtcggtt
    15421 atgacaagga tggctggttc tgggagtata aatctccaac aactagcact tggtatagag
    15481 gtagtcgtgt tgctgctcct gaaacaggat caacaaaccg tctctctatc actctcaagt
    15541 cagacggtca gctaaatgcc agcaataacg atgtcaatct ctttgacaca gtgactctac
    15601 cagctgcggt caatgaccat cttaaaaatg agaagaagat ccttctcaag gcgggctctt
    15661 atggcaatga ccgtacggtt gtcagcgtta aaacagacaa tcaagaaggc gtaaaagcgg
    15721 atgatactcc tgcccagaaa gaaacaggtc cggttgttga cgatagcaag gtgacttatg
    15781 atacgatcca gtctaaggta cttaaggcag taattgacca agccttccct cgtgtcaagg
    15841 aatacacctt gaatggacat actttgccag gacaggtgca acagttcaac caagtcttta
    15901 tcaataacca ccgaatcacc cctgaagtca cttataagaa aatcaatgag acaacagcag
    15961 agtacttgat gaagcttcgc gatgatgctc acttaatcaa tgcggaaatg acagtacgct
    16021 tgcaagttgt ggacaatcaa ttgcactttg atgtgaccaa gattgtcaac cacaatcaag
    16081 tcactccagg tcaaaagatt gatgacgaaa gaaaactact ttcttctatt agtttcctcg
    16141 gcaatgcttt agtctctgtt tctagtgatc aaactggtgc taagtttgat ggggcaacca
    16201 tgtcaaacaa tacgcatgtc agcggagatg atcatatcga tgtaaccaat ccaatgaagg
    16261 atttggctaa gggttacatg tatggatttg tttctacaga taagcttgct gctggtgttt
    16321 ggagtaactc tcaaaacagc tatggtggtg gttcgaatga ctggactcgt ttgacagctt
    16381 ataaagaaac agtcggaaat gccaactatg taggaatcca cagctctgaa tggcaatggg
    16441 aaaaagctta taagggcatt gttttcccag aatacacgaa ggaacttcca agtgctaagg
    16501 ttgttatcac tgaagatgcc aatgcagaca agaacgttga ttggcaagat ggtgccattg
    16561 cttatcgtag cattatgaac aatcctcaag gttgggaaaa agttaaggat atcacagctt
    16621 accgtatcgc gatgaacttt ggttctcaag cacaaaatcc attccttatg accttggatg
    16681 gtatcaagaa aatcaatctc catacagatg gtcttgggca aggtgttctc cttaaaggat
    16741 atggtagcga aggccatgac tctggtcact tgaactatgc tgatattggt aagcgtatcg
    16801 gtggtgtcga agacttcaag accctaattg agaaggctaa gaaatatgga gctcatctag
    16861 gtatccacgt taacgcttca gaaacttatc ctgagtctaa atacttcaat gaaaaaattc
    16921 tccgtaagaa tccagatgga agctatagct atggttggaa ctggctagat caaggtatca
    16981 acattgatgc tgcctatgac ctagctcatg gtcgtttggc acgttgggaa gatttgaaga
    17041 aaaaacttgg tgacggtctc gactttatct atgtggacgt ttggggtaat ggtcaatcag
    17101 gtgataacgg tgcctgggct acccacgttc ttgctaaaga aattaacaaa caaggctggc
    17161 gctttgcgat cgagtggggc catggtggtg agtacgactc taccttccat cactgggcag
    17221 ctgacttgac ctacggtggc tacaccaata aaggtatcaa cagtgccatc acccgcttta
    17281 tccgtaacca ccaaaaagat gcttgggtag ggaactacag aagttatggt ggtgcagcca
    17341 actatccact gctaggtggc tacagcatga aagactttga aggctggcaa ggaagaagtg
    17401 actacaatgg ctatgtgact aacctctttg ctcatgacgt catgactaag tacttccaac
    17461 acttcactgt aagtaaatgg gaaaatggta caccggtgac tatgaccgat aacggtagca
    17521 cctataaatg gactccagaa atgcgagtgg agttggtaga tgctgacaat aataaagtag
    17581 ttgtaactcg taagtcaaat gatgtcaata gcccacaata tcgcgaacgt acagtaacgc
    17641 tcaacggacg tgtcatccaa gatggttcag cttacttgac tccttggaac tgggatgcaa
    17701 atggtaagaa actttctact gagaaggaaa agatgtacta cttcaatacg caggccggta
    17761 caacaacttg gacccttcca agcgattggg caaagagcaa ggtttacctt tacaagctaa
    17821 ctgaccaagg taagacagaa gagcaagaac taactgtaaa agatggtaaa attaccctag
    17881 atcttctagc aaatcaacca tacgttctct atcgttcgaa acaaaccaat cctgaaatgt
    17941 catggagtga aggcatgcac atctatgacc aaggatttaa tagcggtacc ttgaaacatt
    18001 ggaccatttc aggcgatgct tctaaggcag aaattgtcaa gtctcaaggg gcaaacgata
    18061 tgcttcgtat tcaaggaaac aaagaaaaag ttagtctcac tcagaaatta actggcttga
    18121 aaccaaatac caagtatgcc gtttatgtcg gtgtcgataa ccgtagtaat gccaaggcga
    18181 gcatcactgt aaacactggt gaaaaagaag tgactactta taccaataag tctctcgccc
    18241 tcaactatgt taaggcctac gcccacaata cacgtcgtaa caatgctaca gttgacgata
    18301 caagttactt ccaaaacatg tacgccttct ttacaactgg atcggacgta tcaaatgtta
    18361 ctctgacatt gagtcgtgaa gctggtgatc aagcaactta ctttgatgaa attcgtacct
    18421 ttgaaaacaa ttcaagcatg tacggagaca agcatgatac aggtaaaggc accttcaagc
    18481 aagactttga aaatgttgct cagggtatct tcccatttgt agtgggtggt gtcgaaggtg
    18541 tcgaagacaa ccgcactcac ttgtctgaaa aacacgatcc atatacacaa cgtggttgga
    18601 atggtaagaa agtcgatgat gttatcgaag gaaattggtc actcaagaca aatggactag
    18661 tgagtcgtcg taacttggtt taccaaacca tcccacaaaa cttccgcttt gaagctggta
    18721 agacctaccg tgtaaccttt gaatacgaag caggatcaga caatacctat gcttttgtag
    18781 tcggtaaggg agaattccaa tcaggtcgtc gtggtactca agcaagcaac ttggaaatgc
    18841 atgaattgcc aaatacttgg acagattcta agaaagccaa gaaggtaacc ttccttgtga
    18901 caggtgcaga aacaggggat acttgggtag gtatctactc aactggcaat gcaagtaata
    18961 ctcgtagtga ttctggtgga aatgccaact tccgtggtta taacgacttc atgatggata
    19021 atcttcaaat cgaagaaatt accctaacag gtaagatgtt gacagaaaat gctctgaaga
    19081 actacttgcc aacggttgcc atgactaact acaccaaaga gtctatggag gctttgaaag
    19141 aggcggtctt taacctcagt caggccgatg atgatatcag tgtggaagaa gcgcgtgcag
    19201 agattgccaa gattgaagcc ttgaagaatg ctttggttca gaagaaaacg gctttggtag
    19261 cagatgactt tgcaagtctt acagctcctg ctcaggctca agaaggtctt gcaaatgcct
    19321 ttgatggaaa cttatctagt ttatggcata catcatgggg cggaggagat gtaggcaagc
    19381 ctgcaaccat ggtcttgaaa gaaccaactg aaatcactgg acttcgttac gttccacgtg
    19441 gatcaggttc aaatggtaac ttgcgtgatg tgaaacttgt tgtgacagat gagtctggca
    19501 aggagcatac ctttactgca actgattggc cagataacaa taagccaaaa gacattgatt
    19561 ttggtaagac aattaaggct aagaaaattg tccttacagg tactaagact tacggagatg
    19621 gtggcgataa ataccaatct gcagcggaac tcatctttac tcgtccacag gtagcagaaa
    19681 cacctcttga cttgtcaggc tatgaagcag ctttggctaa agctcagaaa ttaacagaca
    19741 aagacaatca agaggaagta gctagtgttc aggcaagcat gaaatatgcg acggataacc
    19801 atctcttgac ggaaagaatg gtggaatact ttgcagatta tctcaaccaa ttaaaagatt
    19861 ctgctacgaa accagatgct ccaactgtag agaaacctga gtttaaactt agctctatag
    19921 tttccgatca aggtaagacg ccagattata agcaagaaat agctagacca gaaacacctg
    19981 aacaaatctt gccagcaaca ggtgagagtc aatctgacac atccctcttc ctagcaagtg
    20041 ttagtctagc cctatctgct ctctttgtag taaaaacgaa gaaagactag tatttagtaa
    20101 aacctcttaa caagattacg gaagcagtct ctatcttttc caatgaggtt tatagtacag
    20161 aaaaagcctg agaagatgtc ttctcaggct tttgttaagc acataaatac aatagtgcta
    20221 tgacaaaatc acccagaaaa atctgggtga taaatgttat ggttgtgctg gttgaggatt
    20281 ctgattttgt tgatcagggg ttgtatttga ttgttgcgta ttattgttag gattggtagt
    20341 cgtactatta tttgtgcttg gagtggttga gctagactgt gaagttgaac tatctgatga
    20401 tgagcttgaa ctttcagttg atgggggttg ttgtggagca ggtgagttcc acgtagaacg
    20461 agcaccattt ttaaatacga attctccatt tctgtagagc ccctctggta tattccaatc
    20521 ttctggattg cttccttcag acaggtaggt catcatagag cggtaaactt tggcagcgac
    20581 cgtaaggcca ttgcctacaa gtggtgtcag acggttagaa tagcctgtcc atacagccat
    20641 tgaatattta cgcgtatagc cagcaaatag ttcatcaggt gctacaaatt gagaggtctt
    20701 gatgtggttt tcaatttcct cgtctgtata gttagaggtt cctgttttac cagcctgagg
    20761 gagccaagca agataggcat ttcgtccagt tccataagtc aagactgttt tcatcatgtc
    20821 ggtcatcata taggctgtcg tttctttcat ggcacgagtt ccgacattag agaactcttt
    20881 ttcactccca tcactaaaga cgactttatg gatatacatt ggtttatagt aagttccacc
    20941 atttgcaaag gcagcgtaag cagcagccat cttttcacta cttgctccat attttttgtc
    21001 tgattcggtt gtgttacttg aaatggcatt tgagtagtga atacttgggt agtcgattcc
    21061 gagaccattt aggaaagtct tggcgcggtt gagtccgacc ttgtttagag tttccacggc
    21121 tgggacgttt cgcgattgtt gcagggcgta ttgcaaggtg atgttgccaa agtagcccct
    21181 atcccagtta taaacaggag tatttgtccc agggtaatta tagggctcat cgtgaacgat
    21241 agtagcagtt gaatcgtaga caccgtactc caaggcagga gcatagtctg tgatcggttt
    21301 catagttgat ccccagtcgc ggtttgtttc tactgcttgg ttaattccga aggaaacatt
    21361 acttgactga tggcgtgctc ctagctgggc aatgacttta ccgttagaaa catcaacaat
    21421 ggtagaagcg acttgcaatt catcgtctgg ataggcaacg tattcgtctg tattgtaaat
    21481 atcccacaga tgtttttgag cttcttggtc tacatttgtg tagacatcca tcccagttgt
    21541 gagcaggtta tagcctgttt cttcttcaac ttgattgatg acttccttga ggtaattatc
    21601 catgtaagca gggtaattac ttgctgattt gagactttgt agtccatcag taattggtgt
    21661 attgactgct ttctcatact gttcagcaga gatgtagcct tgatttttca tttcagataa
    21721 gaccaagttt cggcggtctt gggctgcttc tggatgtgaa taggggtcat attggtttgg
    21781 tgcctgaggc attccagcca gcaaggctaa ctgaggtaaa cttaaattat tgaggtcttt
    21841 accatagtag ttttgagctg ctgtctgcat tccatagttc ccattagaca tgtagacctt
    21901 atttatatag taggtcaaga tttcttgctt ggttgctttt tgttctaact gaatcgctaa
    21961 ccaagcttcc tgagccttac gagaaatagt ctggtcggaa gtcgaagttg aaaagtaagt
    22021 caacttaatc aactgttggg tgagagttga tccaccttgg agggaattgc tttgcagatt
    22081 gcgcaagaaa gctcccagga tacggatggt atcaatcccc ctgtggtcga agaagcgatg
    22141 gtcttcgata gaaacgattg ccttaaccaa atctgtggga atatcattag cttgggcatt
    22201 gacgcggcgt tcagaaccca agtcagcaat gagttgattt ttattgtcgt agattttact
    22261 agaagttgtt gcaactagtt tactctcgga taggctagga gccttgctaa cgtagtagaa
    22321 aaaaactcct ccgcctaaga caatggctgc gataaccaag cttaagaagc taatgctcag
    22381 atacttgatt aggcgcagaa tcgttggttt gttcatcttg ttttaccacc taataaatgt
    22441 tctttgataa cattgagata aggaatttga gggaaggcac cagccttgat ttcatatcca
    22501 tattctcgaa tatattcaag tggcattgat ttttgtccct tatcttgatg atagaagcga
    22561 atcaaatcga atgccggcaa taagtaggtt tcttgctgag aagaaaagtg aagaaggaca
    22621 aagcagattc cttgttgggc aaggacttgt tccatatgct gaatctgatg tggatgaaaa
    22681 tttttcatcg gaatcgcacg tttttgtttt gtttccttga cttcaaagtc gatgtaatat
    22741 ccattataaa cgccagaata gtccgtcgtt gaagcttgtc gaaaataggc ttcaacaatc
    22801 ttggcacgac ttcgttgtgg atagtccact tgtacgattt gaataggagt tggtttctta
    22861 tgtataacag ccaagccctg agacaaatag tagtcgttgg tagcattgat catcttttca
    22921 aaagacattc ctcgatttgc gaaatttttg ggttgagaaa gagatgtttg ccttttttgt
    22981 gatgaaactt tatgtggata gttgaccata attctcctta ttggtacaat aacatcactt
    23041 tattatacca taatttgcac agaaagggtt aaaaatggct acagctttgg ttttaggcta
    23101 ttctgctttt gatttgggtt tattttctga taaggatcct cgtcttaaat tgattaaaaa
    23161 agcgatacgt aaagatttag aggctatggc agcagatggg gtgtcttggc ttgtgtttac
    23221 aggaagtttg ggttttgaat attgggtttt agaggttgct caggagatga aaaccgaata
    23281 cggtttccag ttggccacca tttttgcttt tgaaaaccat ggggaaaatt ggaatgaagg
    23341 caatcagatg aaactgagtc gctttaagca ggtagacttt gtcaaatatg cttatcctcg
    23401 ttatgaacac aaggggcagt taagagatta tcagcagttt ttgctggaaa acacgactag
    23461 ttcctatctt ttttatgatg aagaaaatga aacaaaacta gcttattttt accaaaagat
    23521 gaaaaatcaa gaggactatt ttataaagag attaacattt gatgaattaa atgaacttgc
    23581 tgaaaatttt tccgaaaatt gaagctttga ccttgatttt tgtttgcctt tttttatata
    23641 ataatactag caagcgagaa tggagagaga catggcaagt attatttttt cagcgaaaga
    23701 tatttttgaa caagagtttg gacgtgaagt ccgtggctat aataaagtag aagttgacga
    23761 gtttttagac gatgtcatca aggactatga aacctatgct gccttggtca agtcacttcg
    23821 tcaggaaatt gcggatttga aggaagaatt aactcgtaaa ccgaaacctt caccagttca
    23881 agcagaaccc cttgaagcgg caattacaag ttctatgacg aattttgata ttttgaaacg
    23941 cctgaataga ttggaaaaag aagtttttgg taaacaaatt ttagataact cagattttta
    24001 agtagttatt tgagatgtgc aatttttgga taatcgcgtg aggagaattg cttctcatga
    24061 ggaaagtcca tgctagcaca ggctgtgatg cctgtagtgt ttgtgctagg cgaaaccata
    24121 agcctaggga cgaggaatcg ttacggcagt tgaaatggct aagtccttgg ataggccaga
    24181 gtaggcttga aagtgccaca gtgacggagt ctttctggaa acagagagag tggaacgcgg
    24241 taaacccctc aagctagcaa cccaaatttt ggtcggggca tggagtacgc ggaaacgaac
    24301 gtagtattct gactgctatc agctagagct gttagtggta gacagatgat tatcgaagga
    24361 agtggtccta gtcacttctg gaacaaaaca tggcttatag aaaattgcat ataggttggg
    24421 gctgagaaat tttctcaacc tcatttttta aagtggacat atagaaaggt cttgcaagac
    24481 tgtaacatga aaaaagaatt taatttaatt gcaactgtgg cagcagggct tgaggctgtc
    24541 gttggtcgtg aagtgcgaga gttgggctac gattgtcagg ttgaaaatgg acgtgttcgt
    24601 tttcaaggag acgtgagagc tattatcgaa accaaccttt ggcttcgggc agcagatcgt
    24661 atcaaaatta tcgtaggaac gttcccagct aagacttttg aagagctatt tcagggagtt
    24721 ttcgctttag attgggaaaa ttatttacca cttggagctc ggttcccgat ttcaaaagct
    24781 aaatgtgtta agtccaaact tcacaatgag cctagtgttc aggctatttc taagaaagct
    24841 gttgtcaaga aattgcagaa acactatgct cgcccagaag gggttcctct gatggagaat
    24901 ggcccagagt ttaagattga ggtctctatt ctcaaagatg tggcaactgt catgattgat
    24961 acgaccgggt ctagcctctt taaacgtggt tatcgtaccg aaaaaggtgg cgctcctatc
    25021 aaggaaaata tggcagcagc cattttacaa ctttctaact ggtacccaga caagcctttg
    25081 attgatccga cctgtggttc ggggactttc tgtattgagg cagttatgat tgctagaaag
    25141 atggcgccag gtcttcgtcg ctcttttgca tttgaggaat ggaactggat cagcgatcgc
    25201 ttgattcaag aagtgcgcac agaagcggct aaaaaagtag aacgtaagct tgagttggat
    25261 atcatgggct gtgatattga tgctcgcatg gtggaaattg ctaaggccaa tgctcaggca
    25321 gctggtgttg caggagatat tacttttaag cagatgcgcg tgcaggattt acgttccgat
    25381 aaaatcaatg gagtaatcat ttccaatccg ccttatggtg aacgtttgtc agatgatgca
    25441 agggtgacca agctctatgc tgagatgggg caagtatttg caccgctgaa aacttggagc
    25501 aaatttatcc tgactagtga tgaagctttt gaaagcaagt atggtagcca agcagataag
    25561 aagcgtaagt tatacaacgg aaccttgaaa gtggatctat atcaatattt tggtcagcgt
    25621 gtcaaacggc aagaggtaaa atagaaaggg atactcatga gtaaaaaaag acgaaatcgt
    25681 cataaaaaag aaggtcaaga accgcaattt gattttgatg aagcaaaaga gctaacagtt
    25741 ggtcaagcta ttcgtaaaaa tgaagaagtg gaagcaggag tcttgcctga ggattccatt
    25801 ttggacaagt atgttaagca acacagagat gaaattgagg cggataagtt tgcgactcgt
    25861 caatacaaaa aagaggagtt cgttgaaact cagagtctgg atgatttaat tcaagagatg
    25921 cgtgaggctg tagagaagtc agaagcttct tcggaggaag ttccatcttc tgaagacatc
    25981 ttactaccct tgcctctgga cgatgaggag caaggcttgg atcctctatt gctagaggat
    26041 gaaaatccaa cagaaatgac tgaagaagtg gaagaggagc aaaacctttc tcgtctggat
    26101 caagaggact cagaaaagaa aagtaaaaaa ggctttattt tgaccgtttt ggcgcttgta
    26161 tcagtaatta tttgtgtcag tgcttattat gtctaccgtc aagtggctcg ttcgaccaag
    26221 gaaattgaaa cttctcaatc aactacagcc aatcaatcgg atgtggatga ttttaataca
    26281 ctttatgacg ccttttacac aaatagcaat aaaacggctt tgaaaaatag ccagtttgat
    26341 aaactgagtc aactcaaaac cttgcttgat aaactggaag gtagtcgtga acatacgctt
    26401 gccaaatcta aatatgatag tctagcaacg caaatcaagg ctattcaaga tgtcaatgct
    26461 caatttgaga aaccagctat tgtggatggt gtgttggata ccaatgccaa agccaaatcg
    26521 gatgctaaat ttacggatat taaaactgga aatacggagc ttgataaagt gctagataag
    26581 gctatcagtc ttggtaagag ccagcaaaca agtacttcta gctcaagttc aagtcaaact
    26641 agcagctcaa gctctagtca agcaagttca aatacgacta gtgagccaca accaagtagt
    26701 tcaaatgaga ctagaagtag tcgcagtgaa gtcaatatgg gtctctcgag tgcaggggtt
    26761 gctgttcaaa gaagtgccag tcgtgttgcc tataatcagt ctgctattga tgatagtaat
    26821 aactctgcct gggattttgc ggatggtgtc ttggaacaaa ttctagcgac ttcacgttca
    26881 cgtggctata tcactggaga ccaatatatc cttgaacgtg tcaatatcgt taacggcaat
    26941 ggttattaca acctctataa gccagatgga acctatctct ttacccttaa ctgtaagaca
    27001 ggctactttg tcggaaatgg cgctggtcat gcggatgact tggactacta agcagtcgtt
    27061 acaaaattct ttcttttcaa aagtaaaaat gataaaataa aacaaatgaa acaagaggag
    27121 tgtcaaatga caaaagctaa ctttggtgtc gtaggtatgg ccgtaatggg tcgtaacctt
    27181 gcccttaata ttgaatcacg tggttacaca gttgctatct acaaccgtag taaagaaaaa
    27241 acggaagatg tgattgcttg ccatcctgaa aagaattttg taccaagcta tgacgttgaa
    27301 agttttgtaa actcaatcga aaaacctcgt cgtatcatgc tgatggttca agctggacct
    27361 ggtacagatg ctactatcca agcccttctt ccacaccttg acaagggtga tatcttgatt
    27421 gacggaggaa acactttcta caaagatacc atccgtcgta atgaagaatt ggtaaactca
    27481 ggtatcaact ttatcggtac tggagtttct ggtggtgaaa aaggtgccct tgaaggtcct
    27541 tctatcatgc ctggtggaca aaaagaggcc tacgaattgg ttgcggatgt tcttgaagaa
    27601 atctcagcta aagcaccaga agatggcaaa ccatgtgtga cttacatcgg ccctgatgga
    27661 gctggtcact atgtgaaaat ggttcacaat ggtattgagt acggtgatat gcaattgatc
    27721 gcagaaagct atgacttgat gcaacacttg ctaggccttt ctgcagagga tatggctgaa
    27781 atctttactg agtggaacaa gggtgaatta gacagctatt tgatcgaaat cacagctgat
    27841 atcttgagcc gtaaagacga tgaaggccaa gatggaccaa tcgtagacta catccttgat
    27901 gctgcaggta acaagggaac tggtaaatgg actagccaat catctcttga ccttggtgta
    27961 ccattgtcac tgattactga gtcagtgttt gcacgctaca tttcaactta caaagaagaa
    28021 cgtgtacatg ctagcaaggt gcttccaaaa ccagctgcct tcaactttga aggagacaag
    28081 gctgaattga ttgaaaaaat ccgtcaagcc ctttacttct caaaaatcat ttcatacgca
    28141 caaggatttg ctcaattgcg tgtagcctct aaagaaaaca actggaactt gccatttgca
    28201 gatatcgcat ctatctggcg tgatggctgt atcatccgtt ctcgtttctt gcaaaagatt
    28261 acagatgctt acaaccgcga tgcagatctt gccaaccttc ttttggacga gtacttcttg
    28321 gatgttactg ctaagtacca acaagcagta cgtgatatcg tagctcttgc ggttcaagct
    28381 ggtgtgccag tgccaacttt ctcagcagct attacttact ttgatagcta ccgttcagct
    28441 gaccttccag ctaacttgat ccaagcacaa cgtgactact ttggtgctca cacttaccaa
    28501 cgtaaagaca aagaaggaac cttccactac tcttggtatg acgaaaaata agtaggtcag
    28561 ccatggggaa acggatttta ttacttgaga aagaacgaaa tctagctcat tttttaagtt
    28621 tggaactcca gaaagagcag tatcgggttg atctggtaga ggaggggcaa aaagccctct
    28681 ccatggctct tcagacagac tatgatttga tgttattgaa cgttaatctg ggagatatga
    28741 tggctcagga ttttgcagaa aaattgagcc gaactaaacc tgcctcagtc atcatgattt
    28801 tagatcattg ggaagacttg caagaagagc tggaagttgt tcagcgtttt gcagtttcat
    28861 acatctataa gccagtcctt atcgaaaatc tggtagcgcg tatttcggcg atcttccgag
    28921 gtcgggactt cattgatcaa cactgcagtc tgatgaaagt tccaaggacc taccgcaatc
    28981 ttaggataga tgttgaacat cacacggttt atcgtggtga agagatgatt gctctgaccc
    29041 gccgtgagta tgaccttttg gcgacactta tgggaagcaa gaaagtattg actcgtgagc
    29101 aattgttgga aagtgtttgg aagtatgaaa gtgcgaccga gacaaatatc gtagatgtct
    29161 atatccgcta tctacggagc aagcttgatg ttaaaggaca aaaaagctac attaaaactg
    29221 tgcgtggtgt tggatatacc atgcaagaat agaaaagcag ttgcagttgt gtaactgctt
    29281 ttttgaggag tttttatata ttgacatgca gtcgggtctt tgctacaatc gttttaggag
    29341 gatatataga tgaaactttt gaaaaaaatg atgcaagttc tactagcagt ctttttcttt
    29401 ggtttgctag ggaccagtac agtatttgca gatgattctg aaggatggca gtttgtccaa
    29461 gaaaacggaa gaacctacta caaaaagggg gacctcaaag aaacctactg gcgagtgatt
    29521 gatggtaagt actattattt tgattctcta tctggagaga tggttgtcgg ttggcaatac
    29581 ttagaaattc ctggaacagg ttatcgtgat gatttatttg ataatcgtcc agtcttcgaa
    29641 attggccttc aggagaagtg gtactatttt ggacaagatg gtgctttgct agaacaaaca
    29701 gataaacaag tactagaggc aaaaacgtct gaaaatacag gaaaagtata cggtgaacaa
    29761 tatcctctat ctgctgaaaa gagaacttat tattttgata ataattatgc tgtaaagaca
    29821 ggctggattt atgaagaggg tcattggtat tatttaaata agctaggaaa ttttggcgat
    29881 gattcttaca atccactacc aattggtgaa gttgctaagg gttggactca agattttcat
    29941 gttactattg acattgatag aagcaaacct gctccatggt actacctaga tgcttcaggt
    30001 aagatgctta cagattggca aaaagtaaac ggaaaatggt attattttgg ctcctctggt
    30061 tctatggcaa caggttggaa atatgtacga ggcaaatggt attacttaga taataaaaat
    30121 ggtgatatga aaacaggatg gcaatacctt ggtaacaagt ggtactatct tcgttcatca
    30181 ggagcaatgg caactggctg gtatcaagat ggttcaactt ggtactattt agatccttct
    30241 aatggagata tgaaaatagg ttggacaaaa gtaaatggaa aatggtatta tctcaattca
    30301 aatggagcaa tggttacagg tagtcaaact atcgatggta aagtttataa tttcgcctca
    30361 tccggtgagt ggatttaata ttggagggta tatagatgaa aattttgaaa aaaatgatgc
    30421 aagctggact aacagtaatt ttctttggtt tgctagctac gaatacagta tttgcagata
    30481 actctgaagg atggcagttt gtccaagaaa acggtagaac ctattacaaa aagggggacc
    30541 tcaaagaaac ctactggcga gtgattgatg gtaagtacta ttattttgat tctctatctg
    30601 gagagatggt tgtcggctgg caatatatcc cgtttccatc taaaggtagt acaattggtc
    30661 cttacccaaa tggtatcaga ttagaaggtt ttccaaagtc agagtggtac tacttcgata
    30721 aaaatggagt gctacaagag tttgttggtt ggagaacatt agagattaaa actaaagaca
    30781 gtgttggaag aaagtacggg gaaaaacgtg aagattcaga agataaagaa gagaaacgtt
    30841 attacacgaa ctattacttt aatcaaaatc attctttaga gacaggttgg ctttatgatc
    30901 agtctaactg gtattatcta gctaaaacag aaattaatgg agaaaactat attggtggtg
    30961 aaagacgtgc aggttggata aatgatgctt cagcttggta ctacctagat ccagcaactg
    31021 gtattatgca aacaggttgg caatatctag gtaataagtg gtactacctc cgttcctcag
    31081 gagcaatggc cactggctgg tatcaggaag gtaccacttg gtattattta gaccacccaa
    31141 atggcgatat gaaaacaggt tggcaaaacc ttgggaacaa atggtactat ctccgttcat
    31201 caggagctat ggcaactggt tggtatcaag atggttcaac ttggtactac ctaaatgcag
    31261 gtaatggaga catgaagaca ggttggttcc aggtcaatgg caactggtac tatgcttata
    31321 gctcaggtgc tttggcagtg aatacgaccg tagatggcta ttctgtcaac tataatggcg
    31381 aatgggttcg gtaatgacag ggcgcttata attatattag aacactcgct taatcaaaga
    31441 agtgactttg ttgtacttga cctgacgatt ggggagatta aaagcatgtg aaatctacac
    31501 atgctttttc gttagttatg agtgttgaac ttgactaacc attgacaaaa gataagagca
    31561 atcaaaataa gatacaaaaa tatcaatgct aactgcaccg aaacgcccga tgctatggat
    31621 agggctaata gggagagcag ggacggggcg agaatgtcta ctgtgctcag agcagagccc
    31681 accgtcccca tcttgtcact ggggatatgg gcaaagacag ctgcttgtag gcgagggctg
    31741 agaatgccga taatcgtaga actgatgaaa ctggtcacga aaattacgat ataggcttga
    31801 caaaggatac tagttattag agccaatagg gaaatctcac agaaaaccaa gaggtgcgtg
    31861 aaacggatat ttttaaatag attactgctg gtcatattgc caaggatagc tccacaagac
    31921 atgatgatgg agagcagggc gaagagttgc ccaatgctca gttgtgctag tgtctgttgg
    31981 atgaagcgga gggcaatcag ccgaggggac acatccaaga tggcaatcat actggtcgtc
    32041 agaaacagta gtatgacggt atcttttaac ctcaggagca cagccagcga ctgtttaaga
    32101 tgttgaaaat atttcttaaa actcagtgct gtatgtgaca tttcaatcct tttttcaacc
    32161 tcatacaagg tatgtcgaat aacatacagg cccaaaaagg caatgacaaa agtcagagtg
    32221 ttgataaggg aaatagtttg aatacttata acattgataa ggaatgcacc agccagattg
    32281 gcgacaatgc ggacaaccct catcagagac tgcctgaacc ccatggcatc atgcaggtcg
    32341 tccttaatta cactgatgta gagggcgttc atcatgtagg cagacaggta gctcaaggtg
    32401 tcagagatga ggttgatgat gacaatcata atgactgaaa ctaatgaaaa gggcgacaag
    32461 acgagaaata ctgtcaaaat agcgtagagc aggattttga ttttggcaat acagagtgcg
    32521 tgtttgactc tattttcttg aaaatctcct agaatcccga gaaagagttg cgatagtagg
    32581 ggaaccatct ctgaaatgga aatcagtaaa attgctagcg gagcgaaatt gtactgagcc
    32641 acataattga taatggcgag gtagaaaata ctgtcgccga atgatgaaaa acagctgttt
    32701 agcagcaact gtctatatat gttgtttgtc caaaataatt tcatactttt ctccttatgt
    32761 ctgcacatcg aatgggaatt tattatttaa tagacaccaa tataccccca tgcttcctat
    32821 tccgaagtca aacaaatcta ctttaccact atctccttga aacatcagta aaccgtgatc
    32881 atctcgttca gaacatgcta aaattacctg ttggattttt ttcatcagta atttgttttg
    32941 attgtagaca gtggtttgta gtaagctagt tattccgtgg caatatcctg tcatcatttt
    33001 tagatgatgg ttaaaaacaa attcttgata tttactaata atatctttgt ttccgtcaca
    33061 atcgtacata caaagatata agattattcc ggaaattcct tcgcaccaag aaagttgtaa
    33121 atttcctaaa ttttcagtat tagaagtaac tttttctaat aattttttta attcagcatg
    33181 gaatgtatgg agatcattat agaacataga aggttctaag actttagaat aggcaaataa
    33241 agcatatgca attccacaat atccatgagc aaagctatag tctaaaatgc tttctttggc
    33301 tgtatctctt tgtaggaaat aatgataaat ttccccatat actattgtgg atgaaagttt
    33361 taagagtatt gtcattggta aattgatagt attttattaa tgataatagg acaccagatt
    33421 ttcccaatgc aaagtctatt tcctctagag tatcataatt ctctattaat ttcttagcgg
    33481 tgacgtttgc taattgtaga tagtattgat ttttactgat agaaaataaa tgtaggagta
    33541 accatagata gcctgcatca ccaaatagca gtgagtattg atattcatat gtgtgtgtcg
    33601 ttctaattgt cactgccttc ttgatgatag agagtatttt agaatccagt tctggggggt
    33661 acaatttatt tagaggaaac agtagtccag ctattccgtg ttggaatgat aaattactta
    33721 cgaaatttcc aaattctgtt gaagaagtga tccttccttc agaatttaca gttttatcta
    33781 gtaaaaatcg tagaacacta ttacattctg ttttgtaatc atatttatgt tcctctattt
    33841 ttttaatttt gactaaatta tttgtttgac ttaaatttag tagatattgt aaccaaaata
    33901 gttgctcatc tgtgagctga ccttgagtat gtgatagttc tagtaattga ataggatcct
    33961 ttaggtagat tcaatcaatc tataacacaa caacataata atacttttag gataacttgt
    34021 caaatattat agtaaaatga aataa
//
LOCUS       DAVKLW010000017        33835 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKLW010000017 DAVKLW010000000
VERSION     DAVKLW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.017"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..539
                     /locus_tag="VW324_000802"
     CDS             <1..539
                     /locus_tag="VW324_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726472.1"
                     /translation="LIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGIL
                     IIYFIWLLIEGLKYIRKINYIIQNREIIYFYSQVKVVDKLTDSESKKYGEYIDLYESC
                     RQNNLSVLLNFLQNSDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNK
                     EDAEKLLNEIDKKLEISR"
     gene            595..1290
                     /locus_tag="VW324_000803"
     CDS             595..1290
                     /locus_tag="VW324_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726473.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1302..1718
                     /locus_tag="VW324_000804"
     CDS             1302..1718
                     /locus_tag="VW324_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726474.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
     gene            complement(2533..2625)
                     /locus_tag="VW324_000805"
     CDS             complement(2533..2625)
                     /locus_tag="VW324_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9726475.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            2786..3757
                     /gene="ruvB"
                     /locus_tag="VW324_000806"
     CDS             2786..3757
                     /gene="ruvB"
                     /locus_tag="VW324_000806"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9726476.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            3846..4310
                     /locus_tag="VW324_000807"
     CDS             3846..4310
                     /locus_tag="VW324_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9726477.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            4541..5299
                     /locus_tag="VW324_000808"
     CDS             4541..5299
                     /locus_tag="VW324_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9726478.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            5308..6111
                     /locus_tag="VW324_000809"
     CDS             5308..6111
                     /locus_tag="VW324_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9726479.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            6133..7392
                     /gene="rseP"
                     /locus_tag="VW324_000810"
     CDS             6133..7392
                     /gene="rseP"
                     /locus_tag="VW324_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9726480.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            7405..9258
                     /locus_tag="VW324_000811"
     CDS             7405..9258
                     /locus_tag="VW324_000811"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9726481.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            9358..10737
                     /gene="bglA"
                     /locus_tag="VW324_000812"
     CDS             9358..10737
                     /gene="bglA"
                     /locus_tag="VW324_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9726482.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            10929..12737
                     /gene="glmS"
                     /locus_tag="VW324_000813"
     CDS             10929..12737
                     /gene="glmS"
                     /locus_tag="VW324_000813"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9726483.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYSMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            12856..13905
                     /locus_tag="VW324_000814"
     CDS             12856..13905
                     /locus_tag="VW324_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9726484.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(14005..17802)
                     /gene="spuA"
                     /locus_tag="VW324_000815"
     CDS             complement(14005..17802)
                     /gene="spuA"
                     /locus_tag="VW324_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9726485.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYTSSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTIHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            18219..18632
                     /gene="rpsL"
                     /locus_tag="VW324_000816"
     CDS             18219..18632
                     /gene="rpsL"
                     /locus_tag="VW324_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9726486.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            18652..19122
                     /gene="rpsG"
                     /locus_tag="VW324_000817"
     CDS             18652..19122
                     /gene="rpsG"
                     /locus_tag="VW324_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9726487.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            19547..21628
                     /gene="fusA"
                     /locus_tag="VW324_000818"
     CDS             19547..21628
                     /gene="fusA"
                     /locus_tag="VW324_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9726488.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPVGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            21732..26123
                     /locus_tag="VW324_000819"
     CDS             21732..26123
                     /locus_tag="VW324_000819"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9726489.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLIMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            26225..26488
                     /locus_tag="VW324_000820"
     CDS             26225..26488
                     /locus_tag="VW324_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9726490.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            26481..26759
                     /locus_tag="VW324_000821"
     CDS             26481..26759
                     /locus_tag="VW324_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9726491.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            26780..26932
                     /locus_tag="VW324_000822"
     CDS             26780..26932
                     /locus_tag="VW324_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726492.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            26954..28195
                     /locus_tag="VW324_000823"
     CDS             26954..28195
                     /locus_tag="VW324_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9726493.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            28205..28435
                     /locus_tag="VW324_000824"
     CDS             28205..28435
                     /locus_tag="VW324_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9726494.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            28703..29425
                     /locus_tag="VW324_000825"
     CDS             28703..29425
                     /locus_tag="VW324_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9726495.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            29642..30976
                     /gene="pepC"
                     /locus_tag="VW324_000826"
     CDS             29642..30976
                     /gene="pepC"
                     /locus_tag="VW324_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9726496.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(31032..31943)
                     /locus_tag="VW324_000827"
     CDS             complement(31032..31943)
                     /locus_tag="VW324_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9726497.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(31967..32770)
                     /locus_tag="VW324_000828"
     CDS             complement(31967..32770)
                     /locus_tag="VW324_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9726498.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(32798..33787)
                     /gene="manX"
                     /locus_tag="VW324_000829"
     CDS             complement(32798..33787)
                     /gene="manX"
                     /locus_tag="VW324_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9726499.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYTKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         9679 a         6436 c         7892 g         9828 t
ORIGIN      
        1 tattaatagt gacaaatttg ttatccatta ttcattattt tggagccaaa aatttaatag
       61 tttcttttaa tgaaacttat cctcgtattc aatccagtgt tggtatcatg tggctaccac
      121 tgtttggtat tctgataatc tattttattt ggctcttaat agagggatta aaatatatca
      181 gaaaaataaa ttatattatt caaaataggg aaataatcta tttttattcg caggtaaaag
      241 ttgtagataa acttactgat tctgaatcga aaaaatacgg tgaatatatt gatttgtacg
      301 aatcgtgtcg tcagaacaat ttaagtgttc tacttaactt tcttcaaaat tcggatgata
      361 aacgaggagt tattgctagt aactcagtga gaattatgtt tgaatctttg gataaagcag
      421 accaaattgc ggccctgaat atttgtagaa actatgtagc acgagaaaat gttttaaata
      481 aagaagatgc agaaaaattg ttaaatgaaa ttgataaaaa acttgaaatt agcagatagg
      541 acaattcaaa tagagttgag taaaaagttt gaaaagtgaa acaaggagaa agctatgcca
      601 gtaaatgaat atggtcagat gattggtgag tcaatggaag gttatacacc cggtgcactg
      661 ccttctattg atttcttaga agggcgttat gctaggatag aggctctctc ggtagaaaaa
      721 catgcggagg atttgctagc tgtttatggc ccggatactc ctcgggagat gtggacctat
      781 ctttttcagg agccagtagc agatatgggg gagctggtta gccttttaaa tcagatgttg
      841 gctcgtaagg accgttttta ctatgcaatc atagacaagg cgactggtaa ggctttggga
      901 actttttctc tcatgcgcat tgaccagaat aaccgagtaa tagaagtcgg agcagtcact
      961 ttttctccag agctaagggg tacacggata ggaactgagg ctcagtatct cctagctcgc
     1021 tatatttttg aggagctaaa ctatcgtcgc tacgagtgga aatgcgatgc cttaaatctg
     1081 ccatccagac gagttgcgga gcgtttggga tttgtctatg aaggaacctt ccgccaggcg
     1141 gtggtttata aggggcgtac gagagatacg gattggttgt ctatgattga tatggactgg
     1201 cctcaagtca aagatcgttt ggaaacatgg ctgcgtcctg aaaattttga taaaaatgga
     1261 cagcagtaca agagcttgag agaactttaa gaggtgttga gatgattact attaaaaagc
     1321 aagaaattgt caagctagag gatgttttgc atctctatca ggctgtcggt tggacaaatt
     1381 atacccatca accagagatg ctggagcatg ccttatcgca ttcattagta atttatctgg
     1441 cacttgatgg tgatgctgtg gtgggcttga ttcgtttggt tggagatgga ttctcatcag
     1501 tttttgtaca ggatttgatt gttttaccaa tctatcagcg tcaagggatt ggtagtgccc
     1561 taatgaaaga ggccttaaaa gattacaaag atgcttatca agtccagctg gtgacagaag
     1621 agacagaaaa aaacgtggga ttttatcgtt ctatgggctt tgaaatctta tccacctata
     1681 attgtatagg gatgacttgg gcgaatcgag aaaaataaca aaacttgttt tttcttaagc
     1741 aaaatttaag gatggtctag tattatatag tcattaaata aagacctcct aactttattt
     1801 aataaaatcc taaacttttt tcatcacaat ctcctaatga agccacccaa tcaggtggct
     1861 tttttgcgtg gtgaggtgct ggtgatagaa attttttgca aaatagtaaa attttagaaa
     1921 agttaagcta gttttaagtt tcgtcttgta tcatgtagtt attaaataaa gacctcctaa
     1981 ctttatttaa taaaatccta aacttttctt tttcataata atctccctta actccaccca
     2041 atcaggtgga gttttttagc tctatttcag gcttttgggg actattctaa aaataatttt
     2101 tcgatatttt tcggtatttt tcggattttg gtcggggaat tggcggggac ttttttagcg
     2161 aatataacta agaaataggt ctgtggtcgc ttcagctagt tcaaactcaa tttgattgta
     2221 agtgactgtt aattgaagaa ctggaactac tgtaataatg attttgcttg tctagtagtg
     2281 gcgtattaaa tgagaagtga catgtaagat tgtctcttcg tttacgaaat caaaatttct
     2341 atgaaattga tagaatccct aacgttctct taatcttaac cgtcttgtcg tacagcggtt
     2401 gaagaaaatg taaacctttg acaaaattcg tgaactatgg gataataaaa aggaattgag
     2461 tactagttct atatcatagg ctagaaaaga ccccaagctc acgaccaaat aagcttgggg
     2521 tcttttttga cactatttgt ccttgtttag ccatttatcg actatacgaa gaacgacacc
     2581 gaccacaatt ggtccgatga tagttttgag tactaattcc atcatgggct atctcacctc
     2641 ctttcgtagg cggtgtagca gtgtcgtaga atattatacc acataagtgc taaacttagg
     2701 agtcacccaa tcaggtggct tttttgtttg tggcttggtt ttttgatata atagagccat
     2761 gagtagaatt ttagataatg agataatggg ggatgaggag ttagtagaac gcacgctccg
     2821 tcctcagtat ttacgtgaat atattgggca ggataaggtt aaggaccagc ttcaaatctt
     2881 tatagaagct gccaaaatgc gagatgaagc gctggatcat gtcctcttat ttgggcctcc
     2941 aggtttgggg aaaacaacca tggcttttgt cattgccaat gaactgggag tcaatctcaa
     3001 gcaaacgtct ggtcctgtta tcgaaaaagc gggtgatttg gtagcgattt tgaatgagtt
     3061 agagcctgga gatgtcctct ttattgacga gattcatcgc ttacccatgt cagtggaaga
     3121 agtgctttat agtgccatgg aggacttcta catcgatatt atgattgggg ctggtgaagg
     3181 cagtcgcagt gttcatttgg agttgcctcc ttttaccttg attggtgcga cgactcgggc
     3241 tggtatgctc tcaaatccac tacgggcacg ttttgggatt acaggtcata tggagtatta
     3301 tgcccatgct gacttgacag aaattgtcga gcggacggca gatatttttg agatggaaat
     3361 cactcatgag gcagcatctg agttggccct acgtagtcgt ggaacccctc gtattgccaa
     3421 tcgtctcctc aagcgcgtgc gcgattttgc ccagataatg gggaatgggg taatcgatga
     3481 tattattacc gataaggctt tgactatgct ggatgttgac catgaaggtt tggactatgt
     3541 ggatcaaaaa atccttcgta ccatgattga gatgtacagt ggaggacctg ttggtctagg
     3601 aactctttct gtcaatatcg ccgaagagcg tgagacagtt gaagacatgt atgagcctta
     3661 cttgattcaa aaaggtttta tcatgcggac acggtctgga cgggtggcga ctgctaaggc
     3721 atatgagcac ttaggttatg aatacagtga aaaataagca agaaatctta gaggctttta
     3781 gagaaaatcc agatatgatg gccattctga cgatcatccg agaccttggt ttgaaagact
     3841 cgtagttggc agcaggttct gtcagaaatt tcatctggaa tcttttgtca gacaaatccc
     3901 cttttgatca tgaaacagat atagatgtga ttttctttga tccagatttt tcttatgagg
     3961 aaaccttatt actggagaaa aagctgagag aggattttcc tcagtaccag tgggaattga
     4021 aaaatcaggt ctatatgcat cagcacagtc ctcacactgc ttcctatacc agttctcgtg
     4081 atgctatgag taagtatcca gaacggtgta cggcagttgg actgcgcttg aatgaagaat
     4141 cagattttga actctatgca ccttatggtt tggaggatat tttgaatttt caagttcgtc
     4201 caactcctca tttcttagaa aatgaagacc gaatggaact ctatcaaaca cgtttatcca
     4261 agaaaaattg gcaggagaaa tggaaaaatt tgatttttaa aaatacttaa ggaaacttta
     4321 agctagggag tgtacactaa gttcataagt taagaagacc ttaacttaaa ctcctaaaac
     4381 tttttcataa taatctccct ataaaaataa agtcgcccaa tcaggcggct tatttttttg
     4441 aaaaatgggc atggtgcctg agaataaata gcttagtgat agaagaaaat ggggaaatat
     4501 ggtataatga aacgatagat ttttgaatag gaataagatc atgtttggat tttttaagaa
     4561 agataaggct gtggaagtag aggttccgac acaggttcct gctcatatcg gcatcatcat
     4621 ggatggcaat ggccgttggg ctaaaaaacg tatgcaaccg cgagtttttg gacataaggc
     4681 gggcatggaa gcattgcaaa ccgtgaccaa ggcagccaac aaactgggcg tcaaggttat
     4741 tacggtctat gctttttcta cggaaaactg gacccgtcca gatcaggaag tcaagtttat
     4801 catgaacttg ccagtagagt tttatgataa ttatgtcccg gaactacatg cgaataatgt
     4861 taagattcaa atgattgggg agacagaccg cctgcctaag caaacctttg aagctttaac
     4921 caaggctgag gaattgacta agaacaacac aggattgatt cttaattttg ctcttaacta
     4981 tggtggacgt gctgagatta cacaggcgct taagttgatt tcccaggatg ttttagatgc
     5041 caaaatcaac ccaggtgaca tcacagagga attgattggt aactatctct ttactcagca
     5101 tttgcctaag gacttacgag acccagactt gattatccgt actagtggag aattacgttt
     5161 gagcaatttc cttccatggc agggagccta tagtgagctt tattttacgg acaccttatg
     5221 gcctgatttt gacgaagcgg ccttgcagga agctattctt gcctataatc gtcgtcatcg
     5281 ccgatttgga ggagtttagg aggaaatatg acccaggatt tacagaaaag aaccttgttt
     5341 gcagggattg ccctggctat tttcctacca attttaatga ttgggggcct cttgcttcag
     5401 atagcaatcg gaatcatagc catgctagcc atgcatgaac ttttgaagat gagaggtcta
     5461 gagaccatga cgatggaggg cctcttgacc ctctttgcaa cctttgcatt gaccattccc
     5521 ttggagaatt acctgacttt tttgccagtt gatgggaatg tggttgccta tagtgttttg
     5581 atttcaatca tgttaggaac gaccgttttt agcaagtctt atacgattga ggatgcggtt
     5641 ttccctcttg ctatgagctt ctacgtgggc tttggattta atgctttact agatgctcgt
     5701 gttgcaggtt tggacaaggc tctcttagcc ttgtgtatcg tctgggcgac agacagtggt
     5761 gcctatcttg ttgggatgaa ctatgggaaa cgaaagttag caccaagggt atcgcctaat
     5821 aaaacccttg agggtgcctt gggtggtatt ttaggagcaa ttttagtaac cattatcttt
     5881 atgatagttg acagtacagt tgctcttcca tatggaattt acaagatgtc agtctttgct
     5941 attttcttta gcattgctgg acaatttggt gatttactag aaagttcgat caaacgtcat
     6001 tttggtgtta aggattctgg gaaatttatc cctggacatg gtggtgtttt ggatcgtttc
     6061 gatagtatgt tgcttgtatt tccaatcatg cacttatttg gactctttta atcaaaagac
     6121 ggaggaaacg ctatgctcgg aattttaacc tttattctgg tttttgggat tattgtagtg
     6181 gtgcacgagt tcgggcactt ctactttgcc aagaaatcag ggattttagt acgtgaattt
     6241 gccatcggta tgggacctaa aatctttgct cacattggca aggatggaac ggcctatacc
     6301 attcgaatct tgcctctggg tggctatgtc cgcatggccg gttggggtga tgatacaact
     6361 gaaatcaaga caggaacgcc tgttagtttg acacttgctg atgatggtaa ggttaaacgc
     6421 atcaatctct caggtaaaaa attggatcaa acagccctcc ctatgcaggt gacccagttt
     6481 gattttgaag acaagctctt tatcaaagga ttggttctgg aagaagaaaa aacatttgca
     6541 gtggatcacg atgcaacggt tgtggaagca gatggtactg aggttcggat tgcaccttta
     6601 gatgttcaat atcaaaatgc gactatctgg ggcaaactga ttaccaattt tgcaggtcct
     6661 atgaacaatt ttatcttagg tgtcgttgtt ttttgggttt taatctttat gcagggtggt
     6721 gtcagagatg ttgataccaa tcagttccat atcatgcccc aaggtgcctt ggccaaggta
     6781 ggagtaccag aaacggcaca aattaccaag attggctcac atgaggttag caactgggaa
     6841 agcttgatcc aagctgtgga aacagaaacc aaagataaga cggcaccgac tttggatgtg
     6901 actatttctg aaaaggggag tgacaaacaa gtcactgtta cacccgaaga tagtcaaggt
     6961 cgttaccttc taggtgttca accgggggtt aagtcagatt ttctatccat gtttgtaggt
     7021 ggttttacaa ctgctgctga ctcagctctc cgaattctct cagctctgaa aaatctgatt
     7081 ttccaaccgg atttgaacaa gttgggtgga cctgttgcta tctttaaggc aagtagtgat
     7141 gctgctaaaa atggaattga aaatatcttg tacttcttgg caatgatttc catcaatatt
     7201 gggattttta atcttattcc gattccagcc ttggatggtg gtaagattgt gctcaatatc
     7261 ctagaagcca tccgccgcaa accattgaaa caagaaattg aaacctatgt caccttggcc
     7321 ggagtggtca tcatggttgt cttgatgatt gctgtgactt ggaatgacat tatgcgactc
     7381 ttttttagat aatcgaggaa tattatgaaa caaagtaaaa tgcctatccc aacgcttcgc
     7441 gaaatgccaa gcgatgctca agttatcagc catgctctta tgttgcgtgc tggttatgtt
     7501 cgccaagttt cagcaggtgt ttattcttat ctaccacttg ccaaccgtgt gattgaaaaa
     7561 gctaaaaaca tcatgcgcca agaattcgaa aagattggtg ctgttgagat gttggctcca
     7621 gcccttctta gtgcagaatt gtggcgtgaa tcaggtcgtt acgaaaccta tggtgaagac
     7681 ctttacaaac tgaaaaaccg tgaaaaatca gactttatct taggtccaac tcacgaagaa
     7741 acctttacag ctattgtccg tgattctgtt aaatcttaca agcaattgcc actcaacctt
     7801 tatcaaattc agcccaagta tcgtgatgaa aaacgcccac gtaatggact tcttcgtaca
     7861 cgtgagttta tcatgaagga tgcttatagt ttccacgcta actatgatag tttggatagt
     7921 gtttatgatg agtacaaagc agcctatgag cgtattttca ctcgtagtgg tttagacttc
     7981 aaggctatta ttggtgacgg tggagccatg ggtggtaagg atagccaaga atttatggcc
     8041 attacatctg ctcgtacaga ccttgaccgc tgggttgtct tggacaagtc agttgcctca
     8101 tttgacgaaa ttcctgcaga agtgcaagaa gaaatcaagg cagaattgct caaatggata
     8161 gtctctggtg aagataccat tgcttactca agtgagtcta gctatgcagc taacttagaa
     8221 atggcaacaa acgagtacaa accaagcaac cgtgttgtcg ctgaagaaga agttactcgt
     8281 gttgcaacgc cagatgttaa atcaattgat gaagttgcag ctttcctcaa tgttccagaa
     8341 gaacaaacga ttaaaaccct cttctacatt gcagatggtg agcttgttgc agcccttcta
     8401 gttggaaatg accaactcaa cgaagtcaag ttgaaaaatc acttgggagc agatttcttt
     8461 gacgttgcta gcgaagaaga agtggcgaat gttgttcaag caggatttgg ttcacttgga
     8521 ccagttggtt tgccagagaa tattaaaatt attgcagatc gtaaggtgca agatgttcgc
     8581 aatgcagttg tcggtgctaa cgaagatggc taccacttga ctggtgtgaa cccaggccgt
     8641 gattttactg cagaatatgt ggatatccgt gaagttcgtg agggtgaaat ttccccagat
     8701 ggacaaggtg tccttaactt tgcgcgtggt attgagatcg gtcatatttt caaactcgga
     8761 actcgctact cagcaagcat gggagcagat gtcttggatg aaaatggtcg tgctgtgcca
     8821 atcatcatgg gatgttacgg tatcggtgtc agccgtcttc tttcagcagt gatggagcaa
     8881 cacgctcgcc tctttgttaa caaaacgcca aaaggtgaat accgttacgc ttggggaatc
     8941 aatttcccta aagaattggc accatttgat gtgcatttga ttactgtcaa tgtcaaggat
     9001 gaagaagcgc aagccttgac agaaaaactt gaagcaagct tgatgggagc tggttatgaa
     9061 gtcttgacag atgaccgtaa cgaacgtgtc ggtgttaaat ttagcgatag tgacttgatt
     9121 ggtcttccaa tccgtatcac tgttggtaaa aaagcggccg atggcattgt agaagttaag
     9181 atcaaggcaa ctggtgacac cattgaggtt catgcagata acgtgcttga aacgcttgaa
     9241 atcctaagca agaaataaaa actataatca gaagaaaaac aaggaaaaaa tgtaactagt
     9301 ttttaccttg ttttttctgt ataatgggag aaatgagtag ataaagaggt aaatgttatg
     9361 ctaagatttc caaaggattt tgtctgggga tcctctactt ctggaccgca gacagaagga
     9421 cgtgtagctg gtgacggtaa gggagacaat ctctgggatt actggttcca agtggagcca
     9481 aatcgttact ataatgggat tggttcagat aagacatcga ctttttatga aaattgggag
     9541 cgggatattg agcttttgtt agagactggt cacacagtct ttcggacttc tattcagtgg
     9601 tcacggattt ttccacaagg ctgtggaaaa gtcaaccctc aaggtgtgga tttttatcgt
     9661 aaggtctttg aggctattaa ggctaaagga attcgtctgt tagtcaatct ctatcatttt
     9721 gatttacctt ttgcccttca agaggatggt gatggttggg aaaataaggc gacagtctca
     9781 gcctatgaag actatgctcg tttttgtttt gagacttatg gagatttagt ggatcaatgg
     9841 attaccttta acgagcccat cgttcctgta gaatttggtt atttttatga tgcccattat
     9901 ccacataagg tggatgcaga ggcagctgtt aaggtagcct atcatacaca attggccagc
     9961 agccgggctg ttaaggcttg ccatgaactt ttgcctgatt ccaagattgg gattgtcctc
    10021 aacttgacac cggcttatcc acgtagccag catcctgctg atgtcaaggc agctcgtatt
    10081 gcggcccttt ttcaggccca atctttctta gatccatctg tcttggggac ttatccacag
    10141 gagttggtag aaatcttgca tgaacacggt cttttacctg atgctacaga ggaagagttg
    10201 gaactcattc gtgataatac ggtggacttc cttggtgtga actactatca acctttgcgt
    10261 gttatggctc ctcgatttgc taagcatcca gatagtccac tcttaccaga acatttttac
    10321 gagccttatg tgatgcctgg acgtaaaatc aattctcacc gtggttggga gatttatgag
    10381 caagggattt atgacatcgc ccaaaatatc aaggaaaatt atggcaatat tgagtggatg
    10441 ttgactgaga atggtatggg tgttgaaggg gaagaaaaat tccgtcaaga tggaatgatt
    10501 caagatgatt accgtattga ctttgtaaaa ggtcatcttc gtgaacttca ccgtgccatt
    10561 gaagatggtg ccaactgtaa gggctacttg atttggacct ttattgactg ctggtcatgg
    10621 ctcaacagct ataaaaatcg ctatggtttg gtcgaattag acttggaaac gcaagaacgt
    10681 cgtctgaaga aatcaggcca ctggttcaaa gaattaagcg ataataatgg attttaaaaa
    10741 ggactctgac tgattatccc aatcggtcag agttttttgc ttacaggagc ttaatttgaa
    10801 ctataccaat ttttactctt gacaagtgaa agaaacaagt atatactgtt tttgctgatt
    10861 ctataaaaga tgaattagac tataccaatt ttaaggagaa agcacagctt gcctgtgtcg
    10921 tatatactat gtgtggaatt gttggtgttg ttggaaacac aaatgcaact gatattttga
    10981 ttcaagggct tgaaaagctt gaataccgtg gctatgattc tgcgggaatt tttgtcctag
    11041 atggtgctga taaccatttg gtgaaggcgg ttggtcgtat tgcagaattg tctgccaaga
    11101 cagctggtgt tgagggaaca actggtatcg gacatactcg ttgggctact cacggaaaac
    11161 caactgagga caatgctcac ccacaccgct ctgagacaga acgttttgtc ttggtgcata
    11221 atggggtgat tgagaactat cttgaaatca aggaagaata ccttgcaggt caccacttca
    11281 aggggcagac agatactgaa atagccgttc atttgattgg aaaatttgcg gaagaagaag
    11341 ggctctcagt tcttgaagcc ttcaaaaaag cccttcacat catccgtggt tcttatgcct
    11401 ttgccttggt tgactcacaa gatcctgaag tcatctacgt agctaaaaat aaatcaccac
    11461 ttttgattgg tcttggagaa ggctataaca tggtctgctc agacgccatg gctatgattc
    11521 gtgaaactaa ccaatacatg gaaatccatg accaagagtt ggtaatcgtc aaggctgata
    11581 gtgtggaagt tcaagactat gatggaaatc gtcgtgagcg tgctagctac actgctgagc
    11641 ttgacttgtc agatatcggt aagggaactt acccttacta catgctcaag gaaattgacg
    11701 agcaaccaac tgttatgcgg aaactcatcc aagcctacac agatgaggct ggtcaagttg
    11761 tcgtggaccc agatatcatt aaggctgttc aagacgcaga ccgcatctac atccttgcag
    11821 ctggaacatc ttaccatgca ggatttgctt ctaagaaaat gttggaagaa ttgacagata
    11881 caccagttga acttggaatc tcatctgagt ggggctacag tatgccactt ctcagcaaga
    11941 aaccactctt catctttatc agccaatctg gtgaaacagc ggatagtcgt caagttttgg
    12001 tcaaggctaa tgaaatggga attccaagct taacagtgac aaacgttcca ggttcaaccc
    12061 tctcacgtga agccaactat accatgctcc ttcacgcagg tcctgaaatt gccgtggcat
    12121 caactaaagc ctatacagcg caaatcgcag cccttgcctt ccttgcaaaa gcagtcggag
    12181 aagcaaatgg taatgctaaa gcgcaagcct ttgacctggt tcatgaattg tcaatcgtag
    12241 ctcagtctat cgaatcaact ctttcagaga aagaaaccat tgaagccaag gttcgtgaac
    12301 ttcttgaaac aactcgtaac gccttttaca tcggacgtgg tcaagattac tacgtagcca
    12361 tggaagcaag tctcaaactc aaagagattt cttatatcca gtgtgaaggt tttgcggcag
    12421 gagaactcaa gcacggaacc attgccttga ttgaagaagg aacgcctgtt ttggctctct
    12481 tgtcagatcc agttcttgcc aaccatactc gtggaaatat ccaagaggtc gcagcccgtg
    12541 gtgccaaagt cctcactatc gcagaagaga atgtagccaa agataccgac gatatcgtcc
    12601 ttacgaccgt acatccatac ctctcaccaa tttcaatggt cgtaccaacg caattagtcg
    12661 cttactttgc aaccctccac cgtggcctcg atgtggataa accacgtaac cttgctaaat
    12721 cagtaacggt agaataagct aaaaaagtct agttatctag gctttttcta atgagcaaat
    12781 tggttgcttg tacttaagat tcgtgttaga ataatttttc aaattgaagg acagagatag
    12841 acaaggagaa tcacaatggt agaattggga atttcaacat ttggggaaat aacggagctt
    12901 gaagggactg gacaaactta cagtcatgct gaacgcattc gccagttggt ggcagagatt
    12961 gagctggctg acaaggttgg tttggatgta tatgggattg gtgagcacca tcgggcggat
    13021 tttgcagtat cagccccaga gattgttctg gccgctgggg cagtcaatac caagaaaatc
    13081 cgtttgacca gtgcagtcag cattctgtca agcatggacc cgattcgttt gttccaacag
    13141 tatgccacta tcgatgcttt gtcaaatgga cgagcggaga ttatggctgg aaggggctct
    13201 ttcacggaat ctttccctct gtttggttat gacttgaaag actacgactc tctttttgat
    13261 gagaaattag acttgcttca gttagtcaat gaaaagacca agttagactg gcaaggtcga
    13321 ttgactcaaa cgattgctgg taaagaagtt tatcctcgtc cagttcagga caaattgccc
    13381 ttgtggatag ctacaggtgg tcatgtcgaa tcaacagtga agattgctca ggctggtcta
    13441 ccgattgtat atgctattat tggtggtaat ccacgttatt ttaaaaagtt gattcaggct
    13501 tatcgtgaga ttggaagcga agcgggtcat gctgacaaag acttgaaagt gggagcccat
    13561 tcttgggggt ggattgcgga agatggtgag caggcggtga aagattattt ccatccgact
    13621 aagcaagtgg tggatgctat ttccaaagac cgtccgcact ggcaggaatt gcgttatgag
    13681 caatatttgg agcaggtagg gccaaatggc gccatgtttg ttggcaatcc agatcaggtg
    13741 gccgaaaaat tgattcgcat gatagaggat ttaggattgg accgcttcat gcttcatcta
    13801 ccgcttggtt ctatgcctca tgaccaggtt ctgagagcta ttgaactctt cggaacacaa
    13861 gttgcaccca aagtacgagc ttattttgct atgaaagagg cttaataaaa aatcctaatc
    13921 aagtttatta ggacaacaaa tattgtacga aaattaatcc cccgagctat aatgcttggg
    13981 ggatttttct ataggagggc tagtttagtt ctctttttta ttttttagta agaaaccgag
    14041 accaaggagc gcaaggaggc tgattcctgc aaataggagt ttgttttcgt ttttgattcc
    14101 tgtatttgga agttcagctt gtttatctgg ggttgcaggt atattttcct tgctagagtt
    14161 ttctaccgat tcgtttcgaa ccgcttcttt tacagatgat gcttgtgctt cttgtgctgg
    14221 ttgttcagct tgtgaatcag ctgtagcttg gctaggttta ttttccgcat cagctacttt
    14281 ggcatctggt ttagtagaat caggtctagc ttctggagct gggtcttgat gtgcagggtg
    14341 agaagcttca ttttgatgtt caggcttgga aggggttgag tctggtttct cttctgcagt
    14401 tgactcatgg ctagttccat tttgagagac tcgaagaaca gtagctgtaa gggcattcaa
    14461 tttcaagcct ttttcagtcc attcaagtcc tttcgggttg gcaattccga ctggtcctgc
    14521 ttggttttca tctgccaaaa cttccgcatt tcttagatgt gcaaaggcag ttcccaaatt
    14581 aaattcgcga gctttttcat ccgcattgac aaagactgcg tagatatcgc cgtttggagc
    14641 agtgatttgg tagccaatca ctacatcctc tttttccaca ccattttggc ctgggacagt
    14701 gatgaggtgg acacggtctt tgatatcttg gagactctta agtcggaagg catctgtaga
    14761 ttgacgaagg gcaatcaaac ctttcatata gtcacggctc ttgacatttt caggataagc
    14821 tttaccatct gtagccttag tccagtcaaa cttgttgact gcgtcgctag aatcgtaaga
    14881 atcatggatg aagtaaggat agtcaaatgg gttgccgtcc ttatcacgta acaagtgaga
    14941 tttgtttgga accttatcct ctgctactgg agtcttgtag gctgggtcac ggaattgttt
    15001 agtacgtcca tattcctgac cggagtggat aaatggagtt ccttgagctg tcaagaccat
    15061 gagatttcca agtcgtaaac gacggtggat ttcagcatag ttctcagcct tgcttgggtc
    15121 ttttttgata gactgggcaa tgatgtcaaa gagggtcaag ttatcatggg ctgctatgta
    15181 ttggatgaca tctccagggc tgtcagcttc aaagttagtt ggttgagcaa tgagattttt
    15241 aaagatggtg ttgacatcac gcttgccacc tgtgataaag gcaggttgac cttcgtttgg
    15301 ataaccagat ttgaggttgt tacggatgtc atctgaaaag acagcgacag tatcggtatg
    15361 tttcatccaa tcttggtcag cagctttagt aggcatgttt tcatcaccgg cataggttct
    15421 ccaaccttca ccaagcatga tgaggtttgg attgagggcg cgtgcagcct tgtaagcttc
    15481 ttcgatagaa gcggcgtcat ggtctcccat catatcgaaa cggaatccat ccactttgta
    15541 ggtatcaact aggtatttga tagagtcaac taggagccgt ttggtcatat ggtggattgt
    15601 tcccaagcgt ccaccaccaa agctagttcg aggtgtgcca tcggcatcca taaagtggta
    15661 gtagtttggc tctaggtctt caaagatatc gactttggct gtgtggttat aaacgacatc
    15721 taggatagct cccataccac gtttgtggat ttcgttgatg aggtttttaa attctgcgat
    15781 tcgtttttct ggattcttag gatcgcttga gtacatacca gtcaaggaga agtagttttg
    15841 agggtcatat ccccagttgt agttgctgtt gcttgaagtg tagtcagaca agcgttcatg
    15901 gtttttcaat tcattgacaa agtagtaaga caagactgga aggagctgga tatgggttac
    15961 acccaagtct ttgagatagt ctagtttttc aatgaaggct tcaaaagtcc caaatggttt
    16021 ggtcaagtct tttgcaatgg caggatctga agtgaagtca cgcacatgag cttcgtagat
    16081 aacggcgtct tcacgagtct tgaaattgtg aatcttacca taagtcaagt cttgaggtcc
    16141 gagtttagct ggatctacaa aggcggcttt agccacttta tgggcatcgt caattttggc
    16201 atcgtcgcta ttccaagcag caagagattt agcgtaagga tcgagtgcaa gaacagtttt
    16261 accttgacgc tcgatttggt attgataata gtagccagtg aaatctgtga ttccgagttt
    16321 gtttgtgctg tctagagttt gtttccaagt tcctctttcc cctttttcga gagcgacagt
    16381 tccaactact ttgtcagggt cattcttgtc gtagacaaca acagaaacct tatcagcact
    16441 tggtgaccaa agggtcaaat caacttgttt tccttcttct tttaggtcag ctcccagttt
    16501 gccatcatag ctgtatgtct catctttcag gcgccagctt gttttggtag tgaattggtc
    16561 ggaattgtag ctaacagtat aaggatgttt tgtgtcagag aaatctccgc tgtaggtcac
    16621 tttcttacca gcttcatcga ttgcaacatc ggtaatagtt accttgtttc ctaggtgatt
    16681 agtgatgttg gagtgtttga ggatatcttc ttttttagca ccgacaagtg ttgaaaagct
    16741 actttcaatg ctagaagtgc ctacgtgttg ggctcctgtc atacggatat catggacata
    16801 gtatggattt gtgtaaatcg attcatcatc gtcttttagg aaaatttggc tatgattttt
    16861 caaatctgtg aacttataat tttctttacg gattttcacg tcgtctcctt gtttgctctc
    16921 atctagtaat aaaaatccaa attctcttgc ggcttcatta agaggaatgt cgatatagcg
    16981 gccatatttg cctgtagccg taaagtctgt tccgtcaggc cattgagcgc tacttggatt
    17041 ttttacatct ccccagtacc agagagattt cttgtcatag ttgccatctg tgcggtagta
    17101 gttgacgcga acagttcctg caggctgtgg ctcgtaagag aaaaccttgt aatcttggtc
    17161 taaccaagct tcgttcattt ttggaactag tttttctaca gatttatcgc cggttagatt
    17221 ttttccagct gtattgttga tgaggaagct aattttcttg gcttgttctc cctttaattt
    17281 gacatctagg taatagccgt agtcatcttt cttggcatcc ttgaaggaca aagctccgtt
    17341 tggccagttt tcagatggtt tttcaacatc gtcccaagtc catagtcctt gagcatcctt
    17401 gttttcttca ggaagttttt tgacatggat acggaagtag ttatcttcga tgggctcttc
    17461 tgccttagtt tcagttgtta ctggactagt agaagtagtt ggctctgttg caggtttttc
    17521 ttctgttttc tctagggaag cgtttgcatt tctaggtgaa gaaatatcac tttcgttctt
    17581 accagagcca gctgctgtat cagtaagagg ttgggcaagg gtggttgtat ttctgttgtt
    17641 gggttcgtta gtggttggtg tactttcgtc ggatgagata gttggtgtag ccatggcaag
    17701 caagacaagg cttgcgccga taaggacaga accagttcca tttttgaggg aacggatgct
    17761 gtagaccatt tttttctcag tgtgagatgg tgtttttctc ataatgattc tccaatcaat
    17821 aaatttctat atcagtatag catgtagtaa aacaatatgc aagcgttttc tttgatttgt
    17881 gaaaaaaatt cttacttttg agaagttttc tgtactattt tatatatcag tataaatgac
    17941 ggagataaaa atgtcacata agccaagccg tattattagg aagatgatgt gttcccagct
    18001 atttttattc agaaattgat ctctaacatt accaataaaa aagaaaaata tcttgacaag
    18061 caagggaaaa ttttgttaca ataatagacg gtacttttta cttttggtct ctcaagagtg
    18121 tacagggacg tgctgacaaa tgttgcaaaa gtacacacag atgatatctg tcaccaagtg
    18181 tatcatcacc aaaaataaaa aaacacagga gaatgtagat gcctacaatt aaccaattgg
    18241 ttcgcaaacc gcgtaaatca aaagtagaaa aatctaaatc accagctttg aacgttggtt
    18301 acaatagtca taaaaaagtt caaacaaacg tttcttcacc acaaaaacgt ggtgttgcaa
    18361 ctcgtgttgg aacaatgaca cctaaaaaac ctaactcagc ccttcgtaaa ttcgctcgtg
    18421 tacgtttgag caaccttatc gaagttactg cctacatccc aggtatcgga cacaacttgc
    18481 aggagcacag cgtggtgctt cttcgcggtg gacgtgtaaa agaccttcca ggggtacgtt
    18541 accatatcgt ccgtggtgca cttgatactg caggtgttaa cgatcgtaaa caaggccgtt
    18601 ctaaatacgg tactaaacgt ccaaaagcat aaggaaaggg gataaagaga aatgagtcgt
    18661 aaaaatagag ctccaaaacg tgacgtattg ccagatccgc tatacaattc acaactagtt
    18721 actcgtctta tcaaccgcgt tatgcttgat ggtaaacgtg gtactgctgc ttcaatcgtt
    18781 tacggtgctt ttgagcaaat caaagaagct actggcaacg atgcacttga agtatttgaa
    18841 acagctatgg aaaacatcat gcctgtactt gaagtacgtg cacgtcgtgt tggtggttct
    18901 aactaccaag tcccagttga agttcgtcca gaacgtcgta caacacttgg acttcgttgg
    18961 ttggtaacaa tcgctcgtct tcgtggtgaa cacacaatgc aagaccgtct tgcaaaagaa
    19021 atcttggatg ctgctaacaa cactggtgca gcagttaaga aacgtgaaga tactcaccgt
    19081 atggctgaag ctaaccgtgc attcgcacac ttccgttggt aagataggat gcgaaagcgt
    19141 taagaaagtc ccagataaaa tagggaatcg aagcaggttg cggttgcaac caatgagatt
    19201 catctttttc tccagacttt tagcttgagc tcaactaaat catgatgcta ggaacggtaa
    19261 ggatgcaagg tgaaaatagg aaactgacgc agtattcgac gaatacaagg agttttatct
    19321 ttttcacgca gcatcccgtt ccagctcaca tcggctaact aactttagcc cgggttcaaa
    19381 ttagctaaat cgattagtat tagctataac tcagcttacc atctcgtaag ttgaaaccaa
    19441 caatagcatg aaaacattga gaacgggtag gtcctgccta tccgttttta ttaaaatcgt
    19501 gttataatag aatagaaatc aaaaataaat aggagaaaca aacctcatgg cacgcgaatt
    19561 ttcacttgaa aaaactcgta atatcggtat catggctcac gtcgatgccg gtaaaacaac
    19621 aactactgag cgtattcttt actacactgg taaaatccac aaaatcggtg aaactcacga
    19681 aggtgcgtca caaatggact ggatggagca agagcaagaa cgtggtatca cgatcacatc
    19741 tgctgcgaca acagctcaat ggaacaacca ccgcgtaaac atcatcgaca caccaggaca
    19801 cgtggacttc acaatcgaag tacaacgttc tcttcgtgta ttggatggtg cggttaccgt
    19861 tcttgactca caatcaggtg ttgagcctca aactgaaaca gtttggcgtc aagcaactga
    19921 gtacggagtt ccacgtatcg tatttgccaa caaaatggac aaaatcggtg ctgacttcct
    19981 ttactctgta agcacacttc acgatcgtct tcaagcaaat gcacacccaa tccaattgcc
    20041 agtcggttct gaagatgact tccgtggtat cattgacttg atcaagatga aagctgaaat
    20101 ctatactaac gaccttggta cggatatcct tgaagaagac atcccagctg aataccttga
    20161 ccaagctcaa gaataccgtg aaaaattgat tgaagcagtt gctgaaactg acgaagaatt
    20221 gatgatgaaa tacctcgaag gtgaagaaat cactaacgaa gaattgaaag ctggtatccg
    20281 taaagcgact atcaacgttg aattcttccc agtattgtgt ggttcagcct tcaaaaacaa
    20341 aggtgttcaa ttgatgcttg atgcggttat cgactacctt ccaagcccac ttgacatccc
    20401 agcaatcaaa ggtattaacc cagatacaga cgctgaagaa attcgtccag catctgacga
    20461 agagccattt gcagctcttg ccttcaagat catgactgac ccattcgtag gtcgtttgac
    20521 attcttccgt gtttactcag gtgttcttca atcaggttca tacgtattga atacttctaa
    20581 aggtaaacgt gaacgtatcg gacgtatcct tcaaatgcac gctaacagcc gtcaagaaat
    20641 cgacactgtt tactcaggtg atatcgctgc tgccgttggt ttgaaagata ctacaactgg
    20701 tgactcattg acagatgaaa aatctaaaat catccttgag tcaatcaacg ttccagaacc
    20761 agttatccaa ttgatggttg agccaaaatc taaagctgac caagacaaga tgggtatcgc
    20821 ccttcaaaaa ttggctgaag aagatccaac attccgcgtt gaaacaaacg ttgaaactgg
    20881 tgaaacagtt atctcaggta tgggtgaact tcaccttgac gtccttgttg atcgtatgcg
    20941 tcgtgagttc aaagttgaag cgaacgtagg tgcgcctcaa gtatcttacc gtgaaacatt
    21001 ccgcgcttct actcaagcac gtggattctt caaacgtcag tctggtggta aaggtcaatt
    21061 cggtgatgtg tggattgaat ttactccaaa cgaagaaggt aaaggattcg aattcgaaaa
    21121 cgcaatcgtc ggtggtgtgg ttcctcgtga atttatccca gcggttgaaa aaggtttggt
    21181 agaatctatg gctaacggtg ttcttgcagg ttacccaatg gttgacgtta aagctaagct
    21241 ttatgatggt tcatatcacg atgtcgactc atctgaaact gccttcaaga ttgcggcttc
    21301 actttccctt aaagaagctg ctaaatcagc acaaccagct atccttgaac caatgatgct
    21361 tgtaacaatc actgttccag aagaaaacct tggtgatgtt atgggtcacg taactgctcg
    21421 tcgtggacgt gtagatggta tggaagcaca cggtaacagc caaatcgttc gtgcttacgt
    21481 tccacttgct gaaatgttcg gttacgcaac agttcttcgt tctgcatctc aaggacgtgg
    21541 tacattcatg atggtatttg accactacga agatgtacct aagtcagtac aagaagaaat
    21601 tattaagaaa aataaaggtg aagactaatc cgtcctcact ctagaaggaa gtcacttagt
    21661 ggcttccttt tgtctttaga aaatacctct aaatatggta aaatagtaga agaataatgt
    21721 gaggaaaatg aatgtcaaat agttttgaaa ttttgatgaa tcaattgggg atgcctgctg
    21781 aaatgagaca ggctcctgct ttagcacagg ccaatattga gcgagttgtg gttcataaaa
    21841 ttagtaaggt atgggagttt catttcgtat tttctaatat tttaccgatt gaaatctttt
    21901 tagaattaaa gaaaggtttg agcgaagaat tttctaagac aggcaataaa gctgtttttg
    21961 aaattaaggc tcggtctcaa gaattttcaa atcagctctt gcagtcctac tatagggagg
    22021 ctttctctga aggtccatgt gctagtcaag gttttaagtc cctttatcaa aatttgcaag
    22081 ttcgtgctga gggtaatcag ctatttattg aaggatctga agcgattgat aaggaacatt
    22141 ttaagaagaa tcatcttcct aatttagcca aacaacttga aaagtttggt tttccaactt
    22201 ttaactgtca agtcgagaag aatgatgtcc tgacccaaga gcaggaagag gcctttcatg
    22261 ctgaaaatga gcagattgtt caagctgcca atgaggaagc gctccgtgct atggaacaac
    22321 tggagcagat ggcacctcct ccagcggaag agaaaccagt ctttgatttt caagcgaaaa
    22381 aagctgcagc taaacccaag ctggataagg cggagattac tcctatgatc gaagtgacga
    22441 cagaggaaaa tcgtctggta tttgaagggg ttgtttttga tgtggaacaa aaagtgacta
    22501 gaacaggtcg tgttttaatc aactttaaaa tgacggacta tacttcaagt ttttctatgc
    22561 aaaagtgggt taaaaacgag gaagaggccc agaagtttga cctcatcaag aagaattctt
    22621 ggctccgagt tcgagggaat gtggagatga ataacttcac acgcgatttg attatgaacg
    22681 tacaggatct gcaggaagtt gttcactatg agcggaagga tttgatgcca gaaggtgagc
    22741 gtcgggttga gtttcatgct catactaaca tgtcgactat ggatgctttg ccagaggtcg
    22801 aagagattgt tgcaacagct gctaagtggg gacacaaggc ggttgctatc acagaccatg
    22861 ggaatgtcca gtcctttcca catggctata aggcggctaa gaaagcggga atccagctga
    22921 tctatgggat ggaagccaat atcgtggagg accgtgtccc tatcgtctat aacgaagtgg
    22981 agatggactt atcagaagca acctacgtgg tctttgacgt ggaaacgacg ggactttcag
    23041 ctatctataa tgacttgatt caggttgcgg cctctaagat gtacaagggg aatgttattg
    23101 ctgaatttga tgaatttatc aatcctgggc atcccttgtc agcttttact acagagttaa
    23161 ctggaattac agatgatcat gtcaaaaatg ccaaaccact agaacaagtt ttgcaagaat
    23221 tccaagaatt ttgcaaggat acggtcctag ttgcccacaa tgctaccttt gacgttggct
    23281 ttatgaatgc taattatgag cgtcatggtc ttccaaagat tagtcagcca gttattgata
    23341 cgctggagtt tgctagaaac ctctatcctg agtataaacg ccatggtttg gggcctttga
    23401 ccaagcgttt tggtgtggcc ttggaacatc atcacatggc caactacgat gcggaagcga
    23461 ctggtcgtct gcttttcatc tttatcaaag aggtagcaga aaaacatggt gtgaccgatt
    23521 tagctagact caacattgat ctaatcagtc cagattctta caaaaaagct cggatcaagc
    23581 atgcgaccat ctatgtcaag aatcaggtag gtctaaaaaa tatctttaag ctggtttcct
    23641 tgtctaatac caagtatttt gaaggagtgc cacggattcc gagaacggtt ctagatgccc
    23701 atcgagaggg cttgatttta ggttcagcct gttcagaggg tgaagttttt gacgtggtcg
    23761 tttctcaagg tgtggatgcg gcggttgagg tggccaagta ttatgacttt atcgaggtca
    23821 tgccaccggc tatctatgcg cccttgattg ctaaagagca ggtcaaggat atggaggaac
    23881 tccagaccat tatcaagagt ttgatagagg ttggagaccg ccttggcaag cctgttttgg
    23941 ctacgggaaa tgttcactat atcgaaccgg aagaagagat ttatcgtgaa attatcgtcc
    24001 gtagtttggg acagggtgcg atgattaacc gaactatcgg tcatggtgaa catgcccaac
    24061 cagcaccact tccaaaggct cattttcgaa cgactaatga gatgttggat gaatttgcct
    24121 ttttgggaga ggaactggct cgtaaactgg ttattgaaaa caccaatgcc ttggcagaaa
    24181 tatttgaacc cgttgaagtc gttaagggtg acttgtatac gcctttcatc gacaaggctg
    24241 aagaaacagt tgctgagttg acctataaga aagcttttga gatttatgga aatccgctgc
    24301 cagatattgt tgatttgcgg attgaaaaag aattaacatc catactgggg aatggatttg
    24361 ctgtgattta tctggcatcg cagatgctgg tgcaacgttc taatgaacgg ggttatttgg
    24421 ttggttctcg tgggtctgtc ggatctagtt tcgttgcgac catgattgga attacggagg
    24481 tcaatcctct ctctcctcac tatgtctgtg gtcagtgtca gtacagtgag tttatcacag
    24541 atggttcgta cggttcagga tttgatatgc cccataagga ctgtccaaac tgtggtcaca
    24601 aactcagtaa aaacggacag gatattccgt ttgagacctt ccttggtttt gatggggata
    24661 aggttcctga tattgacttg aacttctcgg gagaagatca gcctagcgcc cacttggatg
    24721 tgcgtgatat ctttggtgaa gaatatgcct tccgtgcggg aacagttggt acggtagctg
    24781 ccaagactgc ctatggattt gtcaagggtt acgagcgaga ttatggcaag ttttatcgtg
    24841 atgcagaagt agaacgcctc gctcaaggag cggcgggtgt caagcggaca acaggccaac
    24901 acccgggggg aatcgttgtt attccgaact acatggatgt ctacgatttt acgcctgtcc
    24961 agtatccagc agatgatgtc acggctgaat ggcagaccac tcactttaac ttccacgata
    25021 tcgatgagaa cgtcctcaaa ctcgatgtac tgggacatga tgatccgacc atgattcgaa
    25081 aacttcagga tttgtctggt attgacccta ataaaattcc tatggatgac gaaggcgtga
    25141 tggcactctt ttctgggact gatgtgctag gggtaacacc tgaacaaatt ggaacgccta
    25201 cgggtatgtt ggggattcca gagtttggaa caaatttcgt acgtggaatg gtagacgaaa
    25261 cccatccgac aacctttgcg gaattgcttc agctgtctgg tctgtcccac ggtactgatg
    25321 tttggttggg gaatgctcag gatctgatta agcaaggaat agcggaccta tcgactgtta
    25381 tcggttgtcg ggacgacatc atggtttacc tcatgcatgc gggtctggaa cctaagatgg
    25441 cctttaccat tatggaacgg gtacgtaagg gtttgtggct aaagatttca gaagaggaga
    25501 gaaatggcta tatcgaagca atgaaggcta ataaggtgcc agagtggtat atcgaatcct
    25561 gtgggaaaat taagtacatg ttccctaagg cccatgcggc agcctacgtt atgatggcct
    25621 tgcgtgtagc ttacttcaag gttcaccatc ctatttatta ctactgtgct tacttctcca
    25681 ttcgtgctaa ggcttttgat atcaagacca tgggtgcggg cttggaggtc atcaagcgca
    25741 gaatggaaga aatctctgaa aaacggaaga acaatgaagc ctctaatgtg gaaatcgatc
    25801 tctatacaac tcttgagatt gtcaatgaga tgtgggaacg aggtttcaag tttggtaaat
    25861 tagatctcta ccgtagtcag gcgacagagt tcctcatcga cggggatacc cttatcccac
    25921 catttgtagc aatggatggt ctgggagaga acgttgccaa gcaactggtg cgggcgcgtg
    25981 aagagggaga attcctctct aaaacagaac tacgcaagcg tggtggactc tcatcaacct
    26041 tggttgaaaa gatggatgag atgggtattc ttggaaatat gccagaggat aaccagttga
    26101 gtttgtttga tgagttgttt taaaaaattg cttaataatc tattaaaaga ggctaacgta
    26161 tatccaatag atttacatta gctttctttt ttgttaaaat agtctatgga aagagggtga
    26221 gagtatgtca aagatgagta taagcatccg tctggatagt gaggttaagg agcaggccca
    26281 acaggtgttt agtaatctgg gaatggatat gacaacagct attaatattt tccttcgtca
    26341 ggcaattcaa tatcagggat taccttttga tgttagacta gacgaaaatc ggaagttgct
    26401 ccaagcgtta acggatttag accaaaatcg taatatgagc cagtcttttg aatcagtctc
    26461 agatttgatg gaggacttac gtgcttaaga ttcgttatca taaacagttt aaaaaagatt
    26521 ttaagttggc tatgaagcgt ggtttgaagg cagaattatt agaagaagtt ttgaattttc
    26581 tggttcaaga aaaagaacat cctgccagat atcgtgatca ttcattgacg gcatccaagc
    26641 attttcaagg agttcgtgaa tgccataccc agccagattg gcttttggtt tataaagtag
    26701 acaagtcgga attgatttta aatttgctga ggacaggcag tcacagtgat ttattttaat
    26761 ctattttaag ggggttctca tgaaactaag aatatttgcg gaagataagc cggctaagaa
    26821 ggtatttgaa tatcaattag aacttgctga tcgtacaatt cttctatcga cagcactctt
    26881 gtcaggtgct attgctttag caggaatctt ttctgctttg aaagaaaaat aaaaatagaa
    26941 aagagaaaac agaatggttt taccaaattt taaagaaaat ctagaaaaat atgcgaaatt
    27001 gttggttgcg aacggaatta acgtgcaacc tggtcacact ttggctctct ctattgatgt
    27061 ggagcaacgt gaattggcac atctaatcgt gaaagaagct tatgccttgg gtgcgcatga
    27121 ggtcatcgtt cagtggacag atgatgtgat taaccgtgag aaattcctcc atgccccgat
    27181 ggagcgtttg gacaatgtgc cagaatacaa gattgctgag atgaactatc ttttggagaa
    27241 caaggctagc cgtcttgggg ttcgttcatc tgatccaggt gccttgaacg gagtggacgc
    27301 tgacaagctt tcagcttctg ctaaagctat gggacttgcc atgaagccta tgcgtatcgc
    27361 aactcaatct aacaaggtta gctggactgt agcagccgct gctggacttg agtgggctaa
    27421 gaaagtcttc ccaaatgctg cgagcgacga agaagcagtt gatttccttt gggatcaaat
    27481 cttcaaaact tgtcgtgtct acgaagcaga tcctgttaag gcttgggagg aacatgcagc
    27541 cattctcaag agcaaggccg atatgcttaa taaggagcaa ttttcagccc ttcactacac
    27601 agcgccagga acagatttaa cacttggttt gccaaagaac cacgtttggg aatcagctgg
    27661 tgctgtcaat gcacagggcg aagaattctt gccaaatatg ccgacagaag aggtcttcac
    27721 agcgcctgac ttccgtcgtg cagatggtta tgtcacttct acaaaaccgc ttagctacaa
    27781 cggaaatatc attgaaggca ttaaggtgac ctttaaggat ggacaaatcg tagatatcac
    27841 tgctgagaag ggtgatcagg ttatgaaaga ccttgtcttt gaaaatgcgg gtgcgcgtgc
    27901 cttgggtgaa tgtgccttgg taccagatcc aagtccaatt tctcagtcag gcattacctt
    27961 ttttaacacc cttttcgatg aaaatgcgtc aaaccacttg gctatcggtg cagcctatgc
    28021 gactagcgtt gttgatggag cggagatgag cgaagaggag cttgaagctg cagggcttaa
    28081 ccgttcagat gttcacgtag actttatgat tggttctaac caaatggata tcgatggtat
    28141 tcgtgaggat ggaacgcggg tacctctttt ccgtaatggg aattgggcaa attaaggaga
    28201 taatatgtta ggaagtatgt tcgttggtct cctagtggga tttttagcag gtgctatgac
    28261 caatcgtgga gagcgaatgg gatgttttgg aaaaatgttt ctcggttgga tcggagcctt
    28321 tctaggtcac ttgctctttg gaacttgggg gccagtttta tcaggaacag ctattatccc
    28381 agcgatttta ggagccatga ttgttttagc tattttttgg agacgaggaa gttaatttcc
    28441 taaatctgat actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca
    28501 aagcactgct ttgaggttgc agatagaact gacgaagtca gctaaaaaca ctgttttgag
    28561 gttgcagata gaactgacga agtcagtaac atatatacgg caaggcgacg ttgacgcggt
    28621 ttgaagagat ttttgaagag tatgaaacga aaaggaggtt ggtcattgta ctagcctcct
    28681 tttgatatga tataatagtt ctatgagatt agataaattt ttagttgcct gtgcggtggg
    28741 aagtcggact gaggtcaaaa acttgctcaa ggctgggcgc gtgactgtaa atggtaaaaa
    28801 agaaaaatca gctaaattgc agattgatga aaaaatagat gagattcgct ttgatgggca
    28861 agtgttggag tatgaagagt ttgtctacta catgatgaac aagcccaaag gagttatctc
    28921 agcgactgag gatcccaagc acagaaccgt tctggacttg ttggatgact tggcgcggag
    28981 taaggaagtt tttccagtag gacgcttgga tattgacacg catggtcttt tgctcttgac
    29041 caataatggt cagctggctc atgttcttct ttcgcccaag cgtcatgtgg acaagactta
    29101 tcaggctcag gtcaagggaa ttatgaccca agaagatgtg gagacattta tcaagggcat
    29161 tccgctcaaa gactttacct gtcagcccgc tagactggag attctttcca cagatgcaga
    29221 aaagaatcag agccaaatcc gtgtgaccat tgcagaaggg aagtttcatc agatcaagcg
    29281 tatggtgggc tactgtggca aggaagtggt ggacttgcaa cgattgacta tgggaacttt
    29341 agtattagat gagaacctgg aacgagggga atggcgtcgc ttgaccaagg aagaattaga
    29401 aattctccgc gctaatatta tttaatttag tttaatactc aatgaaaatc aaagagcaaa
    29461 ctaggaaact agccgcaggc tgtacttgag tacggcaagg cgacgttgac gtggtttgaa
    29521 gagattttcg aagagtataa attcgaaaaa ggtgagggag aatgtcctta ccttttatgt
    29581 tttttagttg cttcctttgt gaaaagagtt ataatagact gtagaataaa aggaggaatc
    29641 tatgaacgcg attcaagaat catttactga taaactattt gccaactatg aagcaaatgt
    29701 aaaataccaa gcgattgaaa atgctgccag ccacaacgga atttttgcag ctctggaacg
    29761 tcgccaaagc catgtagaca acacacctgt tttctcattg gatttaacca aggacaaggt
    29821 cactaaccag aaagcgtctg gtcgttgctg gatgtttgcg gctctcaaca ccttccgcca
    29881 caaactcatc tcgcaataca aattggagaa ctttgagttg tcacaagccc acactttctt
    29941 ctgggataag tatgagaaat caaactggtt cttggagcaa gtcattgcga cttcagacca
    30001 agaattgact agccgcaagg ttagcttctt actccaaaca cctcaacaag atggtggtca
    30061 atgggatatg gtcgtttccc tctttgaaaa atacggtgtc gtgcctaagt cagtttatcc
    30121 tgagtctgtt tcatctagca gcagtcgtga gctaaatgcg atccttaata aattgcttcg
    30181 tcaagatgct caaatcttgc gtgatttgct tgtttctggt gcagatcaag tgactgttca
    30241 agctaagaaa gaagacctct tgcaagaaat ctttaacttt cttgctatgt cattaggact
    30301 tccaccacgc aagtttgact ttgcttatcg cgataaagat aacaactaca aaagtgaaaa
    30361 aggaatcaca ccacaagagt tctacaagaa atatgtcaat cttcctttag aagactacgt
    30421 ttctgttatc aatgctccaa ctgctgataa accttacgga aaatcttaca cagttgagat
    30481 gttggggaat gtggttggta gccgtgcagt tcgttacatc aacgttccaa tggagcgctt
    30541 gaaagaattg gcgattgccc aaatgcaagc aggtgagact gtttggtttg gttctgatgt
    30601 cggccagctc agtaaccgta aggctggaat ccttgcgaca gatgtttatg actttgaatc
    30661 aagcatggac attaaactta ctcaagacaa ggctggacgt ttggactata gcgaaagctt
    30721 gatgacccac gccatggtct tgacaggtgt tgacttggac gaaaatggta aatcaaccaa
    30781 gtggaaggtt gaaaactcat ggggagacaa ggtcggtaca gatggttact ttgttgcctc
    30841 agacgcttgg atggacgaat acacatacca aatcgttgtt cgtaaggaat tgctgacagc
    30901 agaagaacaa gctgcctatg gagcagaacc aattgtactt gcaccatggg atccaatggg
    30961 agccttggct aaataaaagc atagaaaaaa ggaatcagat tttagaacct ggttcctttt
    31021 tagttgcttg attacatgat gtgaagaaca tgtgccacaa tacccactgc gaagagggca
    31081 aggataatag tgattggaga tacttttttc ttaagtaagt acatgcaaag taaagtaagg
    31141 agtagtcctg ataatccagg aatcaacata tccaagtttt gttggaaagt agtaactttt
    31201 tcaggagttt gagacaatcc ttgtcctact tgtgcgaatg cttcttggat acctttagac
    31261 ccttctggca atttatccca atggatataa gccttttcat ctagttgaac tttagaaaca
    31321 tcgaaagcaa atttaatatt tacccagcgt tgaacaagga cagcaagaat gaacatccca
    31381 aggatagaag ctcctttagt gatatcttga aggataccac cagacatatc tttagtgatt
    31441 tctgatccag ccttgtatcc aatctcttga acataccaca agaatgacat acgaatcaag
    31501 ttccatgcaa caaagaagag gagtggcccc aagatattgc cagtaagggc aagtgaagca
    31561 ccgagagatc caaggattgg gcgtactgta aaccagaata ctgggtcacc gataccagca
    31621 agaggtccca tcataccgat tttaacccct tggatagcag cgtcatcgat ttccacaccg
    31681 ttagcacgtt cttcttcaag cgcaagagta acccccatga ctggagcagc tacgtatgga
    31741 tgagtgttga agaactcaag gtgacgctca agagcagcga tttgatcttc ttttttagta
    31801 tagagttttt tgatagctgg aatgagtgta taagcccagc ccaagttttg catccgttca
    31861 aagttccaag acccttgtaa gaaggttgaa cgccaccaaa cttttttacg atctgattta
    31921 gttaattgaa gtttttcagt catgatgttt tcagtccttt cttatcttag tagtcttcta
    31981 ggatatcgcc gattgggtcg ttagaagttg cggctcctcc gccaccattt ccaccagttt
    32041 tagaaaggtg aaggtagata agagcgatag caacaccgat agcaccgaat ccgattagag
    32101 taatatctga cacagcagcg agaacgaaac caagagcgaa gaatggccat acttcacgag
    32161 ttgccatcat gttgataacc atggcgtaac caacggcaac gaccatacca ccaccgatag
    32221 ccataccatc tttgagccag tctggcatgg cactaaggat actttgtaca gtttcagttg
    32281 gtaccataag gagaagagct gcaggaagcg cgatacgaag tccttggaag agtagcgcaa
    32341 tgaaatgcgc acgctccaca gcgccgaagt cacctttttt agcggcagca tctgcagtat
    32401 gaaccaaacc aactgaaatt gtacgaacaa tcattgtcaa gaaaagtcca gctacggcaa
    32461 gagggatagc aaccgcttgg gcaacaccga taccagtctt ggtaaagtca ccaccaagaa
    32521 ccatgataat ggcagcagcg acagaagcaa gtgcagcatc aggagcgata gcagcaccga
    32581 tatttgacca accaagggca atcatttgaa gcgatccacc gaggataatc cctgcttcca
    32641 agtgacctgt tacaagccca ataagggtac aggctacaag tggttggtga aattggaact
    32701 ggtcgaggat gccttcaaga cctgcaaaga aggctacaac gactactaaa accatagaaa
    32761 taatagacat gtttaaaatc ctttcataaa taatggctta tttgacattg gctttgttaa
    32821 tcaagtcaaa caaatctttt ttagaatcat ttggtacttt acggacatca aattcaacac
    32881 ccaagtcacg cattttttca aatgtagcaa catcttcttt gtccatagac aaaacggtat
    32941 tgaccaatgt tttacctgtt gagtgagcca tagaaccaac gttaagagtc ttgattggca
    33001 cgccgccttc gatggcacga agggcatctt gaggtgtttc aaacaagata agggcatgtg
    33061 tttctccaaa acgtgggtct tttgaaatct caatcagttt ttgaattgga accacgttag
    33121 ccttgacatt acctggagct gcttgtttaa tcaattcttt acgaaggtcg tctttagcca
    33181 cgttatctga agcaacgatg atacgatttg cttttgaatc tggagtccaa gcagttgcaa
    33241 cctgaccgtg aagtagacgt gtgtcaagac gggcaagatt gattttcaat ttaccgtctc
    33301 cgataacagt tccttctggg atagcagttt gggcaactgg agcagctgca gcgcttgcaa
    33361 cttcttcaac tggatttagc tcttctggaa gagctttgat gccatctttg gcttctttaa
    33421 tgatattagc agcgactttt tctacacctg cagcagcgtc catgaggcgc tctgtgtagg
    33481 cttgaatcaa catcggtaag ttaagtcctg tgatgatggc aaacttacgc tcaggatttt
    33541 ctcccatcac gcgactagct tggttaaatg gagaaccact ccaaaggtca gccaaaacta
    33601 gaacctcatc ttctgcgtca aatgcagcaa cagcgttatt aaacttagtg tatagatcat
    33661 caggaccttc atttggcata aaggtcacaa cttgaacctt ttcttgttca ccaaagatca
    33721 tagatcctga ctgatgaata cccgcagcaa attcgccgtg gctcgcaata atgattccga
    33781 tactcattat tgtcattcct ccttttttgt tttagttttc atttaagaaa acttt
//
LOCUS       DAVKLW010000018        33700 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKLW010000018 DAVKLW010000000
VERSION     DAVKLW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.018"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..599
                     /locus_tag="VW324_000830"
     CDS             <1..599
                     /locus_tag="VW324_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726500.1"
                     /translation="SFLVFVYISPIFFVIFLYKIIVGSINVRRCLIKIKNYKFYMIVL
                     NRIPLHTFMVYFSSVLLWIMYLTKPVDVSNALPLPIILPLEKINPFYMILGYWLLNKR
                     CEYQLHQYLYPVMRGSSHDGRIYALPEELFIWNRNRSQKIGKWKRDRRYNTTTDLVEA
                     QIGYSKFPFFLLATKWSVDPYDQNWTIGEKAYLTSRWK"
     gene            775..2163
                     /locus_tag="VW324_000831"
     CDS             775..2163
                     /locus_tag="VW324_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9726501.1"
                     /translation="MKSKTVLVTSLAAVTFGLVSLVDTAGALPFSAKTVQADTQTLTT
                     AAKYYILVPKDSEVFKNWRGTANRPIIWATKTTPELSYFQESARNYIANENKSGANYI
                     EWKGTVEEFKEAIKNLTDKKLTPNPAPIPQSDKKPTPDPTPNPQSTVPSGWSGSSYYQ
                     NGTKVTSKWIFDKNYNSYFYLDASGNYVQNAWEGDYYLKSGGYMAKKEWIYDKNYGAY
                     YYLTPDGYYARNTWVGNYYLKSNGKMAKSEWIYDKNYSSYYYLTSEGSYARNTWVGDY
                     YLKSNGKMAKGEWIYDNNYSSYYYLTSEGSYARNTWVGDYYLKSNGKMAVSERTPDGY
                     QVDGSGKWISSEGTHTASSSQATSLGISLYNVNDAMTYIDIALPSTPSSSYQVAYDFA
                     LVDANTGSVSTQTQFTVTYNPSLRVFQTRHFFSATPDGSYRFVIKGKGQDGKVYDGQS
                     VVFRVKNHRFVG"
     gene            2222..3238
                     /locus_tag="VW324_000832"
     CDS             2222..3238
                     /locus_tag="VW324_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEU9726502.1"
                     /translation="MNKKKLLVCAVALSTVGVVSTIDTAGVLPFSAQTVHAEITRTEN
                     HEVKSKITPGSENGSIYVDMTVENVPVHYHVYIYADIVNAKTGQIPRKDTFNERFDRS
                     SVPITKQGWTNFANVPDGEYYLQFHGEDDYRSVLYKFIGKSATFTVKDQKYVNPLDSS
                     TPKETEKPTATETSKPATNSSSGQTGWSGSSYYKAGVKVVNQWIFDVQYNSYFYLNAS
                     GNFVQNAWSGSYYLKSGGYMAKSEWIYDNNYQSYYYLTAEGSYARNAWSGSYYLKSDG
                     KMAKSEWIYDSSYQSYYYLTSEGSYARNTWVGDYYLKSDGKMAVNERTPDGYKVDGSG
                     KWVR"
     gene            3379..4941
                     /locus_tag="VW324_000833"
     CDS             3379..4941
                     /locus_tag="VW324_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726503.1"
                     /translation="MKKFLLATTLLLTLVLGGCQYLPWNKSAETPSDSTSETSVATPE
                     IDYSSYNKVLDDYEKVAKGTLPTGMDVNPLASQVKSSQGFYTDVVYHKTDLNNDGVDE
                     LLLALEMKSGEKSLLDIRTLKDEKVIRLTNQENRLDQIGERMTVGILPDNSLLYRGAG
                     TATSHIYAHYQFSEDGQSLVKDKEAQELADLGVGSPISLETLSWKSVSDKLPGGSSAD
                     KGKPSVDYSPYNSILKDYEFILDYKSEVKDNINPTANIYAGMAERSQAFTYAMVDMDK
                     NGIDELIVSSKNGSEILDIFTLKDQKVIRLTNAENKMQMLGTRFKAYLLENGNLYTQW
                     NPFAGDPQTIHTIWKLNSSGDKLEKVKEAKTEDEVSPGTRMNLSSLTWKSVTEKFAAT
                     ASSTSKTASGKMDINAIKNGDFSSIEGRWSNGHGGEDLVFSKDGLIAPEKFKVDLTES
                     EFVNGFLRARYSAPDFGIFIQFVPAGVRIPDGTSRGELVTDASDISKDRIIFHAAFPL
                     HGDPRQFLYRVE"
     regulatory      5145..5233
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5324..6646
                     /locus_tag="VW324_000834"
     CDS             5324..6646
                     /locus_tag="VW324_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9726504.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVVLLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            6864..7412
                     /locus_tag="VW324_000835"
     CDS             6864..7412
                     /locus_tag="VW324_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9726505.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            7522..8421
                     /locus_tag="VW324_000836"
     CDS             7522..8421
                     /locus_tag="VW324_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9726506.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEMAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(8446..9720)
                     /gene="serS"
                     /locus_tag="VW324_000837"
     CDS             complement(8446..9720)
                     /gene="serS"
                     /locus_tag="VW324_000837"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9726507.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(9934..10305)
                     /locus_tag="VW324_000838"
     CDS             complement(9934..10305)
                     /locus_tag="VW324_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9726508.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(10308..11672)
                     /locus_tag="VW324_000839"
     CDS             complement(10308..11672)
                     /locus_tag="VW324_000839"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9726509.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLTAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            11997..12782
                     /locus_tag="VW324_000840"
     CDS             11997..12782
                     /locus_tag="VW324_000840"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9726510.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12876..13310
                     /locus_tag="VW324_000841"
     CDS             12876..13310
                     /locus_tag="VW324_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9726511.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            13310..14284
                     /locus_tag="VW324_000842"
     CDS             13310..14284
                     /locus_tag="VW324_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9726512.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            14345..14569
                     /locus_tag="VW324_000843"
     CDS             14345..14569
                     /locus_tag="VW324_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9726513.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            14688..15662
                     /gene="fabK"
                     /locus_tag="VW324_000844"
     CDS             14688..15662
                     /gene="fabK"
                     /locus_tag="VW324_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9726514.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            15655..16575
                     /gene="fabD"
                     /locus_tag="VW324_000845"
     CDS             15655..16575
                     /gene="fabD"
                     /locus_tag="VW324_000845"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9726515.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            16609..17340
                     /gene="fabG"
                     /locus_tag="VW324_000846"
     CDS             16609..17340
                     /gene="fabG"
                     /locus_tag="VW324_000846"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9726516.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGTFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            17362..18597
                     /gene="fabF"
                     /locus_tag="VW324_000847"
     CDS             17362..18597
                     /gene="fabF"
                     /locus_tag="VW324_000847"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9726517.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEILYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            18600..19085
                     /locus_tag="VW324_000848"
     CDS             18600..19085
                     /locus_tag="VW324_000848"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9726518.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            19082..19504
                     /gene="fabZ"
                     /locus_tag="VW324_000849"
     CDS             19082..19504
                     /gene="fabZ"
                     /locus_tag="VW324_000849"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9726519.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            19516..20883
                     /gene="accC"
                     /locus_tag="VW324_000850"
     CDS             19516..20883
                     /gene="accC"
                     /locus_tag="VW324_000850"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9726520.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            20920..21786
                     /locus_tag="VW324_000851"
     CDS             20920..21786
                     /locus_tag="VW324_000851"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9726521.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            21783..22550
                     /locus_tag="VW324_000852"
     CDS             21783..22550
                     /locus_tag="VW324_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9726522.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKSLEELLEERYQRFRKY"
     gene            22708..22892
                     /gene="briC"
                     /locus_tag="VW324_000853"
                     /pseudo
     CDS             22708..22892
                     /gene="briC"
                     /locus_tag="VW324_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
     gene            22910..23359
                     /locus_tag="VW324_000854"
     CDS             22910..23359
                     /locus_tag="VW324_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726523.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIELYVTIILGLGADVYSL
                     ILTMVLVGSFLLLIWRLKKKKSWNWSFVFSLFATYVVYQILGNFWARYAHLINHRNIH
                     DEYFTVVLSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            23381..23609
                     /locus_tag="VW324_000855"
                     /pseudo
     CDS             23381..23609
                     /locus_tag="VW324_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(23658..24080)
                     /gene="nusB"
                     /locus_tag="VW324_000856"
     CDS             complement(23658..24080)
                     /gene="nusB"
                     /locus_tag="VW324_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9726524.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(24073..24462)
                     /locus_tag="VW324_000857"
     CDS             complement(24073..24462)
                     /locus_tag="VW324_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9726525.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(24484..25044)
                     /gene="efp"
                     /locus_tag="VW324_000858"
     CDS             complement(24484..25044)
                     /gene="efp"
                     /locus_tag="VW324_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9726526.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(25173..26615)
                     /gene="gatB"
                     /locus_tag="VW324_000859"
     CDS             complement(25173..26615)
                     /gene="gatB"
                     /locus_tag="VW324_000859"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9726527.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(26615..28081)
                     /gene="gatA"
                     /locus_tag="VW324_000860"
     CDS             complement(26615..28081)
                     /gene="gatA"
                     /locus_tag="VW324_000860"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9726528.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(28081..28383)
                     /gene="gatC"
                     /locus_tag="VW324_000861"
     CDS             complement(28081..28383)
                     /gene="gatC"
                     /locus_tag="VW324_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9726529.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            28596..30140
                     /locus_tag="VW324_000862"
     CDS             28596..30140
                     /locus_tag="VW324_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9726530.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            30582..31067
                     /locus_tag="VW324_000863"
     CDS             30582..31067
                     /locus_tag="VW324_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU9726531.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENITQDL
                     I"
     gene            31183..31371
                     /locus_tag="VW324_000864"
     CDS             31183..31371
                     /locus_tag="VW324_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9726532.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            31528..31893
                     /locus_tag="VW324_000865"
     CDS             31528..31893
                     /locus_tag="VW324_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9726533.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            31896..33563
                     /locus_tag="VW324_000866"
     CDS             31896..33563
                     /locus_tag="VW324_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9726534.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMSAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         9867 a         6329 c         7640 g         9864 t
ORIGIN      
        1 catctttttt agtttttgta tatatttcac caatcttctt tgtgattttt ctatataaaa
       61 taattgtggg cagtattaat gtaagacgct gtttgataaa gataaagaat tacaaattct
      121 atatgattgt tttgaataga ataccactac atacatttat ggtttatttt tcatcagtat
      181 tgttatggat aatgtatctt acaaaaccag tggatgtgag taatgcttta cctttgccaa
      241 ttatcttacc tcttgaaaag ataaatccct tctatatgat tctaggatat tggctcttaa
      301 ataagcgatg tgaatatcag ttgcatcagt acctttatcc tgtaatgaga gggagttctc
      361 atgacggtag aatttatgca ctaccagagg aactttttat ctggaatagg aatagaagtc
      421 aaaaaattgg taagtggaaa cgagatagaa ggtacaatac gaccaccgat ctagtcgaag
      481 ctcaaattgg ttatagtaag tttccgtttt ttttgctagc aacaaaatgg tcagtggatc
      541 cttatgatca aaattggaca ataggagaaa aggcatattt gacaagtagg tggaaatgat
      601 aaaatagaaa gtctttagtc taataactag gaaaaagatt aagtatactt ttttcataaa
      661 aatttcattt gctaatcaaa taaagtataa acacagatac tcttttcata aatatagtag
      721 agtccatccc ttactgtaag cctaaataat aaaaatttaa aggaggtttt gataatgaag
      781 tcaaaaacag ttttagtaac cagcctagcc gctgtaacat ttggtcttgt gtctcttgta
      841 gatacagcag gagctctacc tttttcagca aaaacggtac aagctgacac acaaacgctg
      901 actacagctg caaaatatta tattcttgtt ccgaaggatt cagaagtatt taaaaattgg
      961 agaggtactg ctaatcgtcc cattatttgg gccacaaaaa ccactccaga attatcttat
     1021 ttccaagaat ctgctcgcaa ctatattgct aatgagaata agtctggtgc caattacata
     1081 gagtggaagg gaactgtaga agagtttaaa gaggcgatta agaatctgac ggataaaaaa
     1141 ctaactccaa atccagctcc aatcccacaa tcggataaaa aaccaacccc agatccaact
     1201 ccaaatccac aatctacagt tccgtctggt tggtcaggat catcttacta ccaaaatggt
     1261 actaaagtca ctagtaagtg gattttcgat aagaactata attcctactt ctacctagat
     1321 gcttcaggta attatgttca aaatgcatgg gagggtgatt actatcttaa gtcaggtggc
     1381 tacatggcta agaaggagtg gatctatgat aagaactatg gagcttatta ttacttaact
     1441 ccagatggct actatgctcg caatacttgg gtgggcaact attatctcaa gtcaaacggt
     1501 aagatggcta aaagcgagtg gatctatgat aagaactaca gttcatatta ctacctaacg
     1561 tcggaaggaa gttacgcccg taatacttgg gtaggtgatt attatctcaa gtcaaatgga
     1621 aagatggcta aaggtgagtg gatttatgac aataactaca gctcttacta ttacttaaca
     1681 tcggaaggaa gttatgctcg taatacatgg gtaggtgatt attatctcaa gtcaaatgga
     1741 aagatggcag ttagcgaacg tacaccagat ggctaccaag tggatggatc aggcaagtgg
     1801 attagttctg agggaactca tacagcaagc agttcacaag caacatcatt aggaataagc
     1861 ctttacaatg tcaatgatgc tatgacctat attgatatcg ctctacctag cactccatcc
     1921 tcatcttatc aagtagccta tgattttgcc ttagttgatg caaatacagg ttcagtttct
     1981 acacaaaccc agtttactgt gacctacaat ccaagcttac gtgtctttca aactcgtcac
     2041 ttctttagtg ccactccaga tggtagttat cgctttgtta tcaaaggaaa aggacaagat
     2101 ggcaaggtat acgatggcca atcagttgtc tttcgtgtaa agaatcatcg ttttgtcgga
     2161 tagatgagtg ataggtttta aacaaataaa tattaaaaat aaaataaagg aaatgaataa
     2221 catgaacaaa aagaaattat tagtatgtgc tgtggcactt tcaacagtcg gagtagtatc
     2281 aacaatcgac acagcaggag ttctcccttt ttcagcacaa acagtgcatg cggaaattac
     2341 tcgtaccgag aatcatgagg taaaatctaa gattacacca ggatcagaaa atggaagtat
     2401 ctatgtggat atgactgttg aaaatgtacc tgtacactat catgtatata tatatgcaga
     2461 tattgtaaat gcaaaaacag ggcaaattcc tagaaaagat actttcaatg aaaggttcga
     2521 tagatcatct gttccgatta caaagcaagg ttggactaat tttgcaaatg ttccagacgg
     2581 tgaatattac ttacaatttc atggagaaga cgattacaga agtgttttgt ataaatttat
     2641 tggcaaatca gcgacattca ctgttaaaga tcaaaaatat gtcaatcctc tagactcttc
     2701 aactcctaaa gaaacggaaa aaccaacagc tactgaaact tcaaaaccag ctactaactc
     2761 tagctcaggt cagactggct ggtcaggttc ttcttactac aaagctggtg tgaaagtagt
     2821 caatcaatgg atttttgatg ttcagtacaa ttcatacttc tacctcaatg cttcaggtaa
     2881 ctttgttcaa aatgcttggt ctggaagtta ctacctcaag tcaggtggct acatggctaa
     2941 gagcgaatgg atttatgaca ataactatca atcatactat tacttgacag cagagggtag
     3001 ctatgcacgt aatgcttggt ctggaagcta ctacctcaaa tcagatggta aaatggctaa
     3061 gagtgagtgg atctatgata gtagctacca atcttactat tacctaacat cagaaggaag
     3121 ttacgcccgt aatacatggg taggtgacta ctacctcaaa tcagatggta agatggcagt
     3181 caacgaacgt accccagatg gctacaaagt agacggctca ggtaagtggg ttcgctagaa
     3241 tgatagcttg tgagaaatgg cttataacaa ataaatagaa acggaaaata atttgtttat
     3301 aggcaacatt ttgatacaat aaaaatagcg aaaggaattg gcacaggctg gttcctttct
     3361 cttagataaa ggagtaatat gaaaaagttt ttattagcca caacactttt actgacacta
     3421 gttctaggag gctgtcagta tttaccttgg aacaaaagcg cagaaacacc atctgattca
     3481 acatcagaaa catctgtagc gactccagag atagactaca gttcctataa taaagttttg
     3541 gatgactatg aaaaagtagc gaaaggcacc ttgccgacag gtatggatgt caatcctctg
     3601 gctagtcaag tgaagtcttc tcaaggcttt tatacagatg tggtttacca caagacggat
     3661 ttgaataatg atggagtcga tgaactttta cttgctcttg aaatgaagag tggcgaaaag
     3721 agtttacttg atattcgtac gctgaaagac gaaaaggtga ttcgtttgac caatcaagaa
     3781 aatcgtttag accagattgg tgagagaatg acggtcggta tccttccaga caattctctg
     3841 ctctatcgtg gagctggtac agcaacgagt cacatctatg ctcactacca atttagtgaa
     3901 gatgggcagt ctttggtaaa ggataaggaa gctcaagaac tagcagatct tggggtcggt
     3961 tcgcccattt ctctagagac cttaagctgg aagtcagttt cagataaact tccaggagga
     4021 agcagtgcag ataaggggaa accaagtgta gattacagtc cttataatag cattttgaag
     4081 gattatgaat ttattttaga ctataagtca gaggttaagg ataacatcaa tccaactgct
     4141 aatatctatg caggaatggc agaacgcagt caagccttta cctatgcaat ggttgatatg
     4201 gacaaaaatg gcattgatga attgattgtc tcttcgaaaa atggtagtga gattttagat
     4261 atttttacac tcaaagatca gaaagttatt cgcctcacca atgctgaaaa taaaatgcag
     4321 atgcttggaa ctcgtttcaa ggcctatcta ttagaaaatg gtaatctcta tacccagtgg
     4381 aatccgttcg caggagatcc acagaccatt cataccattt ggaaattgaa tagtagtggg
     4441 gacaaactag agaaggttaa agaagctaag acagaggatg aagtcagtcc aggtactcga
     4501 atgaatctat ctagtctgac ctggaaatct gtaacagaaa aatttgctgc aacagcatct
     4561 tcaactagca aaacagcttc agggaaaatg gatataaacg ccatcaaaaa tggagatttt
     4621 tctagtattg agggaagatg gagtaatggt catggaggag aagacttagt ctttagtaaa
     4681 gatggcttga tagcaccaga gaaatttaaa gttgacctta cagaatcaga atttgtaaat
     4741 ggttttttac gagctcgtta ttcagcacca gactttggaa tctttattca gttcgtacca
     4801 gcaggtgtta gaataccgga tgggacatct aggggtgagt tggtcacgga tgcttcggat
     4861 attagtaaag atcgaatcat cttccatgct gcatttccgc tacatggtga tccaagacaa
     4921 ttcttatatc gtgtggaata agagtgaaac agcttgattt aataactaat agcaccccac
     4981 gcacatggga atgctcaatc cttcttctac agagtcaaat aagatttttc caaaacacat
     5041 tgacaaaagc caacattttt tgtaaaataa gaatcaatta aataccaaca ccgaatgaag
     5101 tttaatagaa gtggggaatc gtttgatttt ccatgactgt aaatggacgg aactctggag
     5161 agaccgtaaa ggcaccgaag gggcaaggca ggcaactgct caaactctca ggtaaaagga
     5221 cagagctagg atagaccgct ttttagcatt tatctaagca ttccagagta catgtatctt
     5281 gcatgtgctc tttcttttgg ggttgaaacg ataggagaag gaaatgttag aattgcttaa
     5341 atcaatcgat gcttttgctt ggggaccgcc cctcttgatt ttattggtcg gaacagggat
     5401 ttacctaacc atgcggctag gactcttgca ggttttgcgt ctgcccaagg cctttcagct
     5461 tatttttatc caggataagg gacatggtga tgtatccagt tttgcagctc tgtgtacagc
     5521 cttggcatca actgttggaa caggaaatat cataggagtt gcgacggcta tcaaggttgg
     5581 tggaccagga gctctatttt ggatgtggat ggcggctttc tttggaatgg ctaccaagta
     5641 tgcggaagga ctcttggcca tcaaataccg caccaaggac gaccatggtg cagtagcggg
     5701 aggtcccatg cactatatcc ttctagggat gggagaaaag tggcgaccac ttgctgtttt
     5761 gtttgcagta gcaggagtat tggttgttct cttgggaatc ggaaccttca cccaagtcaa
     5821 ctcgattgca gaatctatcc aaaatacaac gacgatttcg ccagccatca cagctctcgt
     5881 cttgtctgtc tttgtagcga ttgcagtctt tggtggactc aagtctattt ctaaggtttc
     5941 aactactgtt gttcctttta tggccctcat ttatatctta ggaactctta cagttatttt
     6001 ctttaatatc ggaaaaatcc ctggcacaat cgctttagtc tttacctcag cttttagtcc
     6061 ccttgctgcg gtaggtggat ttgctggtgc tagcgttcgg atggctattc aaaatggtgt
     6121 ggcgcgtggt gtgttctcaa acgaatcggg tctgggttct gctcctattg cagctgcagc
     6181 tgccaagaca aatgaaccag tagagcaagg tttgatttcc atgacaggaa cctttattga
     6241 taccctcatc atttgtactc taactggttt gaccatcttg gtaactggag tttggagcgg
     6301 tgacttgaat ggggttgcct tgactcagtc agctttctca acagtctttt cacactttgg
     6361 gcctgctctc ttgaccatct tccttgtact ttttgccttt acaacgattc taggttggaa
     6421 ctattacgga gaacgctgtt ttgagttcct ctttggggtt cgctttatct ggctctaccg
     6481 tgtggttttt gtgctcatgg tcttgttagg aggatttatc gagttggata tggtctggat
     6541 tatcgcagat atcgttaacg ccttgatggc tctgccaaac ttgattgccc tcttggtctt
     6601 gtcgccagtc gttattgctg aaactaaaaa gtattttgac aaataatgga atcacacagc
     6661 tcgtgtgatt tttttgccta taaataattt ttatcagcct gatatataat atatattatg
     6721 caagtgattg gaagtgtgat aaactaatat aacaacgaaa atcttatgag agaataaagc
     6781 tttatcttag ataaacttag gaatcaggat tttataagat tttccaataa tattagtgtc
     6841 aacaataaga aaagaggtat cttatgacaa catttactat ccatacagta gagtcagcac
     6901 cagcagaagt gaaagaaatt cttgaaacag tagaaaaaga caacaatggc tatattccca
     6961 acctaatcgg tctcttggcc aatgccccga ctgttttaga agcctaccaa attgtctcat
     7021 ctatccaccg tcgcaacagc ctgacacccg ttgagcgtga agtggtgcaa atcacggcag
     7081 ccgtgaccaa tggttgtgcc ttctgtgtcg caggtcacac agccttttcc atcaaacaaa
     7141 tccagatgaa tgatgacttg attcaagctc ttcgcaatcg tactccaatt gaaacagatc
     7201 ctaaattgga taccctagct aagtttacct tggcagttat caataccaag ggtcgtgtag
     7261 gagatgaagc cttgtctgag tttttagaag ctggctacac tcaacaaaat gccttggatg
     7321 tggtttttgg tgtcagccta gcaatcctct gtaactatgc caacaactta gctaatacac
     7381 caattaatcc agaattgcaa ccttatgcct aattcatatc tgagtaaaat gaagtctgaa
     7441 ataatcggac ttcgtttttt taaatcctca tttaagcgct tttatgttat actgaaacta
     7501 acatgattat tggaggttag gatgaaaaaa ctccccttag tattttcagg ttgtttgcta
     7561 ggtttggcag gagctggaaa tcttatttta gatacgttgc cagttctatc ccatcttttt
     7621 agtctgactg gtttggtttt atggatttac tttctaattc tgcatctctt taattggaaa
     7681 gaaaccaagc aagaattgac caagccccct cttttgtcag gaatggcaac ctttcctatg
     7741 gctgggatga ttttatcgac ctatgtcttt cgcgtattct cttatcttcc tttggtagca
     7801 caagggattt ggtggttttc atttctcttg gatttgacct tgattgctgg ttttaccatc
     7861 aagtttgctt gtccagggcg gagggttcat gccactccaa gttggacggt tctctatgtg
     7921 gggatagcag tggctgcctt gacctatcct ctggtaggta ttatcgaaat ggcctatgcg
     7981 accctgagtt ttggttttct cttgaccttc tatctctatc cccttattta tagcgattta
     8041 aagaaacatc cactcccact agccttgctt ggacaagaag gaatctactg tgctcctttc
     8101 tctctactct tggcttctct agttcgagta ggaggaacca gcctgccgac ttgggtcttg
     8161 attgtcatga ttttggcttc tcaatccttc tttttctttg ttttaactcg tctgcccaac
     8221 attttaaaac aaggttttca accagccttc tcagccctca ccttcccaac cattatcaca
     8281 gcgacctcgc tcaagatggc tcagggaatt ttgaaacttc catttctgga ttacctggta
     8341 ttggctgaaa ccattatatg cctaactatt ttattctttg tactaggtgc ttatctgatt
     8401 tggttacgaa aaaaggtcta gctagaaata gctaaacctt attttttatg gtttgatgac
     8461 ttcagctcca cccatgtatg gacgaagtgc ttctgggatg gtcacagaac catcttcatt
     8521 ttggtaattt tcaagaattg cagccactgt acgtccaact gcaagtccag aaccgttcaa
     8581 ggtatgaagg agtttcacct tgccatctgc ttcatcacgg taacggattt gggcacgacg
     8641 ggcttggaaa tcttctgtgt ttgaacagct tgagatttca cggtaattgt tttgtgctgg
     8701 aatccacact tccaagtcgt aagtcttcgc agctgagaag cccatatctc cagtagagag
     8761 agcaacgaca cggtatggaa ggttgagttt ttgaagaatg ttttcagcgt tggctgtcat
     8821 tttttccaat tcttcgtaag attcttctgg tttggcaaat ttgaccattt caaccttgtg
     8881 gaattggtgc aaacggatca agccacgcgt atcacgaccg gcagaaccag cctcagaacg
     8941 gaatgacgga ctcatggcag tgaagtagat tggaagatct ttgccgtcta agatttcatc
     9001 acggtagtag tttgtcagag gaacttcagc agttggaatc aagacaaagt tggtatcgct
     9061 gagttcaaaa gtatcttcct taaattttgg atactgacca gtaccaaaca tagaatcatg
     9121 gttgactatg taaggtgtga tgacttcagt atagccttct tttccatgtt catccaacat
     9181 aaagttgtag atagcacgtt ccaaacgagc accgaggcct ttatagaaga ggaagcgagc
     9241 gcctgttacc ttaccaccgc gttcccagtc aagaatacca aggtcttcac cgagatccca
     9301 gtgagcttta ggttcgaagt caaactcgcg tggagtaccc caacggcgaa cttccacatt
     9361 gtcgtcttcg tcagccccaa caggaacgct gtcagctggg atatttggaa gagtcgttgt
     9421 aaattctgtc aatttagcat cgatttctgc caattcagca tccaaggctt taacctcagc
     9481 agatagattt tgcatggcag caatcttgtc atctgtattt tccttgttgc gcttagcttg
     9541 ggcaatctca gcagaaactg tgttacgttc tgctttgaga gtttcaacct tgaccaagat
     9601 gttacgacgt ttagcatcga tttctttcat ttcattcaag acagcagcat ctacaccacg
     9661 tgtagctaat ttttctgcga cagcttcaaa atctgtacga atacgtttga tatctaacat
     9721 aagaactcct ttatgaaaaa agcacgcctg acaaaacgtt ggagtggcag ggccacggtt
     9781 ccatccaact tcacaggtgt gcacttgatt gtgtatgtaa ttgttactaa cggtagaatt
     9841 tcacctatcc ctcctatctg ctcgcagtac ccgcagactt tctgaaagaa gaagataacc
     9901 tacttatccg ttgctatgat tatactaaag tttctacttt tttgcaaata gatttttaaa
     9961 tttttggcta attgtctgaa tcagggtcgg aagtttgacg accttgtcat tgcctagttt
    10021 ttcgcgtgca attttgagaa tggcacctga gtcttttgaa gcaaagagga attttccttt
    10081 gtctgtaaag acttcgaagt ggcggctgat tttgcgtcca gtgacattgg ctccaatctg
    10141 attgatatgg ctccaaggaa tctggataaa ttgttcgaca ttgacatctg ggtaaaattc
    10201 caaagcctga tctccgacaa ggaatttccc aactttccca gcgatagaga ggtaggaagt
    10261 gcctgtcgta ctgaggagta ctgttttgtt aagtgattgg gccatgctta gtcttcctta
    10321 ctttctccaa aaaaggcatt gtagagggct ttaattgctg ctttctcttg gtctttattg
    10381 acaacaaaca taatagaaac ttcactagaa ccttgagaca tcatctggat gttgattttg
    10441 ttttcagata gagcgcgtgt cgcagtagca gtcactccga tgtggctctt cattttttca
    10501 ccaacaatca taatgataga aaggtcgtgt tcgatttctg catgatctac tttagccttt
    10561 tgaaccaact gacgcaggat ttcttcttcc ttgatgggag ttagttggcg agaacggaga
    10621 atgatagaaa gatcgtcgat acctgttggc atatgttccc aaccgatgtt aagttcttcc
    10681 aggatttgca gaaccttgcg gccaaatcca acctcacggt tcatgaggta tttcgacatg
    10741 ttaatgctga caaagcctga gtcaccagca attcccacaa ctggaaattc atcattactg
    10801 tgttttagaa cgatacgagt acctggatgg tcagggttgt tggtattctt gataaccaga
    10861 ggaatttttc cacggtaggc aggaagaaga gcctcgtcat gaaggactga gaagcctgca
    10921 taggccaact cgcgcatttc acggtaggtc aactcaggaa tcgagtgtgg ttggtggata
    10981 ataccagggt gggctgcaaa gataccatca acgtccgtaa agttttcata gaggtcggct
    11041 ttgacaccag cagcaatgat agaacctgta atatcagaac ctccacgtga gaaagtacag
    11101 atttgatttt ccttagtgac accaaagaaa ccaggaatga caaggacttc attggtgttt
    11161 gtcaattctt caatcttgtc ataacttgat ggaatgatgc gagcgtgacc aggttcactt
    11221 gtgaccacaa tcccagcttc tctagggtgc atatagcgtg catcgatacc attttggtta
    11281 aagtaggcag cagtcaattt ggcattgtta ttttcaccgg ctgctaggaa agtatcgtag
    11341 agaaattcat tttcttcaat aggaagagtg gccaaggcgt gaatgctttt agaaattttt
    11401 tctagcacag ctggttttag tcccaattca ctaaccatag cagcatagcg gtcgataatc
    11461 cagctttggc tcttgctaat atcgttaccc gcaacatagt cgcggtagta tttaatcagg
    11521 gcatccgtaa ccttagtatc ttcagcattg cgtttaccag gcgcagaaac gactacaaaa
    11581 cgacgctctg aatcgctttt gacgatgttt aaaacttttt ctaattgact agcagaggca
    11641 agagaactac ctccaaattt aacaactttc ataagaactc ctaaagtaag tattttatac
    11701 gattatagca gaaagaaagg cttttttcaa tgaagaaaat aagatgcttt ctagcctaaa
    11761 gcgagccttt cttatcggct gagacacttt cagtcgattt tttcttgctt tctgctccta
    11821 aaaaagatat actttgacag tgaaataatt taacactcaa tgaaaatcaa agagcaaact
    11881 aagaagctag acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg aagctgacgc
    11941 ggtttaaaga gattttcgaa gagtataaac tgcttataaa ataaaaagga gccctgatgg
    12001 aacacattat ttatcagctt gaagaggact tggcaatcct taccttgaac cgtcctgagg
    12061 tcgcaaatgg ttttcatatt cccatgtgtg aggagatttt agaagctctg actttggcag
    12121 aagaaaatcc agctgtgcat tttatcttaa tcaatgccaa tggaaaggtc ttctcagttg
    12181 ggggagattt ggtagagatg aagcgggcag tggatgagga tgatattcca tcattgacaa
    12241 aaatcgcaga gttggtcaat acgatttctt ataaaatcaa gcaaatagcc aaacctgttt
    12301 taatggaagt tgacggtgct gttgcaggtg ccgcagcgaa tatggctgtt gcggcagatt
    12361 tctgtttggc gacggataag gctaagttta tccaagcctt tgttggtgtt ggtttggctc
    12421 cagatgcagg gggaattcat ctcttgagcc gcagtatcgg tgtgacgcgt gctgctcaat
    12481 tagctatgac aggagaggct ttaacggcag aaaaagccct agaatggggg ttggtttacc
    12541 gcgtttctga agctgaaaaa cttgaaaaga cgagagaaca gcttcttaaa aaattaagac
    12601 gtgcttcaag taattcctat gctgccatta agaagttggt ttgggagagc cagtttaaag
    12661 actggcaggg ttacgctact ttagaactga acctacagaa atccttagct caaacagagg
    12721 atttcaaaga aggagtgcga gctcattcgg agagaagaag acctaaattt acaggaaaat
    12781 aaaaaatact tgcatcattc tttgaatttt gatataattc tttctgtcaa atgttttgat
    12841 tgtaaaagtt ttttgaagaa ggagggatat gaaaaatgga ctaccaacga atcaatgaat
    12901 atttaacatc tatatttaac aatgtccttg taattgagga agtgaacttg agaggtagtc
    12961 gttttaagga tatctccatc aaagaaatgc atacgattga tgtcatcggt aaggctccag
    13021 acgtgactcc aagtcaagtg tcaaaagagt tgatggtaac tcttggaact gttacgacaa
    13081 gtttgaacaa tttagagcgt aagggctaca ttgagcgagt tcggtcagaa caggatcgtc
    13141 gtgtggtgca tctgcatttg acaaagaagg gtcgcttgat tcataggctg cataaacgct
    13201 tccacaaggc catggtagaa aaaattattg atggcatgag cgaggaagaa attgctgtca
    13261 tgggtaaagg tttgactaat ctttaccaat ttttggagga tttgaaataa tggcttttgc
    13321 aaaaataagt caggttgctc attatgtgcc agagcaagtg gttacaaatc acgacttggc
    13381 tcagattatg gataccaatg atgagtggat ttcaagtcga acgggaatac gacaaaggca
    13441 tatttcaaga acagaatcta ccagtgattt ggctacagag gttgctaaga aactgatggc
    13501 aaaagctgga ataacaggag aagaactgga ttttatcatc ctagctacca ttactccaga
    13561 ttcgatgatg ccctctacag ctgctcgtgt tcaagctaat attggtgcta ataaagcctt
    13621 tgcttttgac ttaaccgcgg cttgcagtgg atttgtattt gctctttcaa ctgctgaaaa
    13681 gtttatcgct tctggtcgct ttcaaaaagg cttggtgatt ggtagtgaaa ccctctctaa
    13741 ggcagtcgat tggtcggatc gatcaacagc tgtgttgttt ggagatggtg ctggtggtgt
    13801 cttgttagaa gctagcgagc aagagcattt cttagctgag agtctcaata gcgatggaag
    13861 tcgcagcgag tgtttaactt atgggcattc aggtttgcat tctccatttt cagatcaaga
    13921 aagtgcagat tcgtttttga agatggatgg acgcacagtc tttgattttg ccattcgaga
    13981 tgtagccaag tctatcaagc agactattga tgaatctcct atagaggtga cagacttgga
    14041 ttatctgcta cttcatcaag ccaatgaccg tattttggat aagatggcta gaaaaattgg
    14101 tgttgaccga gccaaacttc cagccaatat gatggaatat ggcaatacca gtgcagccag
    14161 tatcccgatt ttactttcag agtgtgtaga acaaggtctc atccctttag atggtagcca
    14221 gactgttctt ctatcaggct tcggtggagg cttgacctgg ggcacgctca ttcttacaat
    14281 ttaggtaatc atgtggtgaa cacattgtta taaaaaaaac tatttatttt aaaggagtcc
    14341 tatcatggca gtatttgaaa aagtacaaga aattatcgtt gaagaacttg gaaaagacgc
    14401 atcagaagta acacttgaat caacttttga tgatttggac gcagattcat tggacttgtt
    14461 ccaagtaatc tcagaaatcg aagatgcttt tgatatccaa atcgaagcag aaaatgactt
    14521 gaaaacagtt ggtgacttgg ttgcttacgt tgaagagcaa gcaaaataag cagaatatta
    14581 gtagaaggag tagggagaaa cccactccct cttgtttagg taatagtttg actgtcaaat
    14641 tatggtgaaa tcgaactatt acgtaagcaa ggaaggttgg aggcactatg aaaacgcgta
    14701 ttacagaatt attgaagatt gactatccta ttttccaagg agggatggcc tgggttgctg
    14761 atggtgattt ggcaggggct gtttccaagg ctggaggatt aggaattatc ggtgggggaa
    14821 atgccccgaa agaagttgtc aaggccaata ttgataaaat caaatcattg actgataaac
    14881 cctttggggt caacatcatg ctcttatctc cctttgtgga agatatcgtg gatctcgtta
    14941 ttgaagaagg tgttaaagtt gtcacaacag gagcaggaaa tccaagcaag tatatggaac
    15001 gtttccatga agctgggata atcgttattc ctgtcgttcc tagtgtcgct ttagctaaac
    15061 gcatggaaaa aatcggtgca gacgctgtta ttgcagaagg aatggaagct ggggggcata
    15121 tcggtaaatt aacaaccatg accttggtgc gacaggtagc cacagctgta tctattcctg
    15181 ttattgctgc aggaggaatt gcggatggtg aaggtgctgc ggctggcttt atgctaggtg
    15241 cagaggctgt acaggtgggg acacggtttg tagttgcaaa agagtcgaat gcccatccaa
    15301 actacaagga gaaaatttta aaagcaaggg atattgacac tacgatttca gctcagcact
    15361 ttggtcatgc tgttcgtgct attaaaaatc agttgactag agattttgaa ctggctgaaa
    15421 aagatgcctt taagcaggaa gatcctgatt tagaaatctt tgaacaaatg ggagcaggtg
    15481 ccctagccaa agcagttgtt cacggtgatg tggagggtgg ctctgtcatg gcaggtcaaa
    15541 tcgcagggct tgtttctaaa gaagaaacag ctgaagaaat cctaaaagat ttgtattacg
    15601 gagccgctaa gaaaattcaa gaagaagcct ctcgctggac aggagttgta agaaatgact
    15661 aaaacagcct ttttatttgc tggtcaaggt gcccagtatc tagggatggg acgggatttc
    15721 tatgatcagt atccgattgt taaagaaacg attgatcgag cgagtcaggt gctcggttat
    15781 gatttgcgtt atctcatcga tacggaagag gacaaactca atcagacccg ctatacgcaa
    15841 ccagccattc tagcgacttc ggttgctatc taccgtttat tgcaagaaaa gggctatcag
    15901 cctgatatgg ttgctggttt gtctcttgga gaatactctg ccttggtggc aagcggcgcc
    15961 ttggattttg aagatgcggt tgccttggta gctaagcgtg gagcctatat ggaagaagcg
    16021 actcctgctg actctggcaa gatggtagca gttctcaata cgccagtaga ggtcattgaa
    16081 gaagcctgtc aaaaagcttc tgaacttgga gtggttactc cagccaacta taacacacct
    16141 gcacaaatcg tcattgctgg agaagtggtt gcagttgatc gagcggttga acttttgcaa
    16201 gaagcaggtg ccaaacgctt gattcctctt aaggtgtcag gtccctttca caccgctctc
    16261 cttgagccag ctagccagaa actagctgaa actctagctc aggtaagttt ttcagatttt
    16321 acttgtcccc tagtcggcaa tacagaagct gctgtgatgc aaaaagagga cattgctcag
    16381 ctcttgacgc gtcaggtcaa ggaacccgtt cgtttctatg aaagtattgg ggtcatgcaa
    16441 gaagcaggca taagcaactt tatcgagatt ggaccgggga aagtcttgtc aggttttgtt
    16501 aaaaaaattg atcaaactgc tcacttagct catgtggaag atcaagcgag tttagtagca
    16561 cttttagaaa aatagactaa aataagtaga agttttgaaa ggaaaaaaat gaaactagaa
    16621 cataaaaata tctttattac aggttcgagt cgtggaattg gtcttgccat cgcccacaag
    16681 tttgctcaag caggagccaa cattgtctta aacagtcgtg gggcaatctc agaagaattg
    16741 ctcgctgagt tttcaaacta tggtatcaag gtggttccca tttcaggaga tgtatcagat
    16801 tttgcagacg ctaagcgtat gattgatcaa gctattgcag aactgggttc agtagatgtt
    16861 ttggtcaaca atgcagggat tacccaagat actcttatgc tcaagatgac agaagcagat
    16921 tttgaaaaag tgctcaaggt caatctgact ggtaccttta atatgacaca atcagtcttg
    16981 aaaccgatga tgaaagccag agaaggtgct atcattaata tgtctagtgt tgttggtttg
    17041 atggggaata ttggtcaagc taactatgct gcttctaagg ctggcttgat tggctttacc
    17101 aagtctgtgg cacgcgaggt cgctagtcgg aatatacgag tcaatgtgat tgctccagga
    17161 atgattgagt ctgatatgac cgctatctta tcagataaga ttaaggaagc tacactagct
    17221 cagattccga tgaaagaatt tgggcaggca gagcaggttg cagatttgac agtattttta
    17281 gcaggccaag attatctaac tggtcaagtg attgccattg atggtggctt aagtatgtag
    17341 cgaaagctag aggtgaaaag aatgaaacta aatcgagtag tggtaacagg ttatggagta
    17401 acatctccaa tcggaaatac accagaagaa ttttggaata gtttagcaac tgggaaaatc
    17461 ggcattggtg gcattacaaa atttgatcat agtgactttg atgtgcataa tgcggcagaa
    17521 atccaagatt ttccgttcga taaatacttt gtaaaaaaag ataccaaccg ttttgataac
    17581 tattctttat atgccttgta tgcagcccaa gaggctgtaa atcatgccaa tcttgatgta
    17641 gaggctctta atagggatcg ttttggtgtt atcgttgcat ctggtattgg tggaatcaag
    17701 gaaattgaag atcaggtact tcgccttcat gaaaaaggac ccaaacgtgt caaaccaatg
    17761 actcttccaa aagctttacc aaatatggct tctgggaatg tagccatgcg ttttggtgca
    17821 aacggtgttt gtaaatctat caatactgcc tgctcttcat caaatgatgc gattggggat
    17881 gccttccgct ccattaagtt tggtttccaa gatgtgatgt tggtgggagg aacagaagct
    17941 tctatcacac cttttgccat cgctggtttc caagccttaa cagctctctc tactacagag
    18001 gatccaactc gtgcttcgat cccatttgat aaggatcgca atgggtttgt tatgggtgaa
    18061 ggttcaggga tgttggtttt agaaagtctt gaacacgctg aaaaacgtgg agctactatc
    18121 ctggctgaag tggttggtta cggaaatact tgtgatgcct accacatgac ttctccacat
    18181 ccagaaggtc agggagctat caaggccatc aaactagcct tggaagaagc tgagatttct
    18241 ccagagcaag tagcctatgt caatgctcac ggaacgtcaa ctcctgccaa tgaaaaagga
    18301 gaaagtggag ctatcgtagc tgttcttggt aaggaagtac ctgtatcatc aaccaagtct
    18361 tttacaggac atttgctggg ggctgcgggt gcagtagaag ctatcgtcac catcgaagct
    18421 atgcgccata actttgtacc aatgacagct gggacaagtg aagtatcaga ttatatcgaa
    18481 gctaatgtcg tttatggaca aggcttggag aaagaaattc tatacgctat ttcaaatact
    18541 tttggttttg gaggccacaa tgcagttctt gctttcaaac gttgggagaa tagataagta
    18601 tgaatttaaa cgatattaaa gacttgatga ctcaatttga ccagtcaagt ttgagagaat
    18661 tttcttataa aaatgggacg gatgagttgc agtttagcaa gaatgaagca agacctgtgc
    18721 ctgaagttgc aactcaagtc gctccagcac ccgttctagc aacaccgagt ccagtagctc
    18781 ctacatctgc tccagcagag actgtagcag aagaagttcc agctccagct gaagcaagtg
    18841 tggctactga gggaaatctt gtagagagtc cacttgttgg agtggtttac ttggctgctg
    18901 gaccagataa acctgccttt gttacagttg gtgatagtgt caaaaaaggt caaacattgg
    18961 taattatcga agccatgaaa gtcatgaatg aaatcccagc tcctaaggat ggtgtggtaa
    19021 cggaaattct cgtctctaac gaagaaatgg ttgagtttgg taaaggattg gtacgtatca
    19081 aatgatcgat attcaaggaa tcaaagaagc tcttccccac cgttatccta tgcttctagt
    19141 ggaccgtgtc ttggaagtga gcgaggatac cattgttgct atcaaaaatg tgaccattaa
    19201 cgaacctttc tttaacggcc actttcctca atacccagtt atgccaggtg ttctgattat
    19261 ggaagccttg gcgcaaactg ctggtgtgtt ggagttatca aaacctgaaa ataaaggaaa
    19321 actggtcttt tacgctggta tggacaaggt taagttcaag aagcaagttg taccaggcga
    19381 ccaattggtt atgacagcga cttttgtaaa acgtcgtggc accatagctg tggttgaagc
    19441 aaaggctgaa gtggatggca agcttgcagc cagtggtacc cttacttttg caattgggaa
    19501 ctaaggaggt tctccatgtt tcgaaaaatt ttaattgcca atcgtggtga aattgcggtt
    19561 cgtattatcc gtgcggcacg tgaattgggg attgcgacgg tagcggttta ttcaactgct
    19621 gataaggaag ctcttcatac gctgttggca gatgaagcag tttgtattgg tcctggcaag
    19681 gcaacagagt cttatctcaa tattaatgca gttctatcag ctgcagtctt gactgaggca
    19741 gaagctattc accctggttt tggatttctc agtgaaaatt ccaaatttgc gaccatgtgt
    19801 gaagaagtag gtatcaagtt tatcggtcca tctggtcatg ttatggatat gatgggggat
    19861 aagatcaatg cgcgtgctca gatgattaaa gcaggtgtgc ctgttatacc aggttcagat
    19921 ggagaagtgc ataactctga agaagctttg attgttgctg aaaaaattgg ctatcctgtt
    19981 atgctcaagg cttcagcagg tggaggtggt aaagggattc gtaaggttga aaaaccagat
    20041 gacctcgttt ctgcctttga aactgcctct agtgaggcca aggccaatta tggcaatggt
    20101 gccatgtaca tagaacgggt tatctatcca gctcggcaca ttgaggttca aatcctaggt
    20161 gatgagcatg gacatgtgat tcacttgggt gaacgggatt gttctcttca aaggaataac
    20221 caaaaggttt tggaagaaag tccctcgatt gcaatcggaa aaacgctgcg tcatgaaata
    20281 ggtgctgctg ctgttcgagc ggcagagttt gttggctatg agaatgcagg aaccattgaa
    20341 tttcttcttg atgaagcaag tagcaatttc tatttcatgg agatgaatac tcgtgttcag
    20401 gtagaacatc cagtaacaga gtttgtttca ggtgttgata tcgttaagga acagatttgc
    20461 attgcggcag gtcagccttt gtctgttaag caagaagata ttgtcctacg cggtcatgcc
    20521 atcgagtgtc gtatcaatgc agaaaaccca gcctttaact ttgctccaag tccaggtaag
    20581 attactaacc tctatctgcc aagtggtgga gttggcttgc gcgtggattc agcagtttat
    20641 ccaggttata ccattccgcc ttattatgat agtatgattg ccaaaatcat agtacacggc
    20701 gaaaatcgtt ttgacgcctt gatgaaaatg caacgtgccc tctatgaatt agagattgaa
    20761 ggagtgcaga ccaatgcaga tttccagctt gacctcattt cagatcgcaa tgtcattgct
    20821 ggggattatg atacttcctt cttgatggaa accttcttac ctaaatatca agaaaaagaa
    20881 taaaatgact ttaagagttt aaaccgaaaa ggggaatcaa tggctctatt tagtaaaaaa
    20941 gataagtata ttcgaatcaa tcccaatcgt tcggttaggg aaaaacctca agctaagcca
    21001 gaggttccag atgaattatt ttcccagtgt ccaggctgta agcataccat ctatcagaag
    21061 gatctgggaa gtgaacgtat ctgtccgcac tgtagctata cctttcgtat ttctgcccaa
    21121 gaacgcttgg ctttgacgat tgatatggga accttcaaag aattgtttac agggattgaa
    21181 agcaaggatc ccttgcattt ccctggttac caaaagaaac tggcatctat gcgtgaaaaa
    21241 acaggtctgc atgaagccgt tgtgacagga actgctctta ttaaaggtca gactgtggct
    21301 cttgggatta tggattctaa ctttatcatg gcttctatgg gtacggttgt aggtgaaaaa
    21361 atcactcgtt tgtttgagta tgcgactgtc gaaaaattgc cagttgtcct attcacagcc
    21421 tctggtggag cccgtatgca ggaaggaatc atgagtctca tgcagatggc taagatctct
    21481 gcggcggtta aacgtcattc aaatgctggt ctcttttacc tgaccatttt gacagatcca
    21541 acgactggtg gtgtgacagc ttctttcgct atggaaggcg atatcattct ggctgaacca
    21601 cagagcttgg ttggttttgc tgggcgtcgt gtgattgaaa atacggttcg tgaaagcttg
    21661 cctgaggatt tccaaaaggc agaattccta ttagaacatg gctttgtgga tgctattgtc
    21721 aaaagaagag acttaccaga tacgattgct agcctagtca gattgcatgg agggagtcct
    21781 agatgaatat tgcaaaaata gtcagagaag cgcgtgagca gagtcgcttg acaaccttgg
    21841 actttgcgac aggcattttt gatgaattta tccaattaca tggtgaccgt tcttttcgtg
    21901 atgatggtgc agttgttggt ggtattggtt ggcttggaga ccaagctgta acagtggttg
    21961 gtatccaaaa aggcaagagt ttgcaagaca acctcaaacg gaattttggc caaccacatc
    22021 cagaaggcta ccgaaaggca ctgcggttga tgaaacaggc tgagaaattt ggccgtccag
    22081 ttgtgacctt tattaataca gcaggtgctt atcctggtgt cggagcagaa gaacgtggtc
    22141 aaggggaagc tatcgctcgc aatctcatgg aaatgagtga cctgaaagtt cctattatcg
    22201 ccattattat cggtgaaggt ggttcaggcg gggctctggc tctagctgtc gcggaccgtg
    22261 tctggatgct ggaaaattct atctatgcca ttctcagtcc agaaggcttt gcttccattt
    22321 tatggaagga cggtactcgc gccatggaag cagcagaact gatgaaaatc acttcgcatg
    22381 aactgttaga aatggacgtg gtggataagg tgatttctga agtaggactt tctagtaaag
    22441 aactgattaa gagtgtcaaa aaagaactcc aaacggagct ggctagactt tcacaaaaat
    22501 cgctagaaga gttgctggaa gaacgctatc aacgatttag aaaatactaa gaatgactaa
    22561 atgagactag aaaggtctca ttttttatgg aaaaatgcaa ttcaggttga aaaattgata
    22621 gttgaggaga tgggctataa aatagacaaa aaagaaatat actataaatg tgagcctaca
    22681 acaatctatt ttaagaaggt acttgcaatg acaggtacag aaacatttac agttatttca
    22741 actgaggact tggagcaaac ttcaggtggt cttgctgttt gggaagatgg atatagtaga
    22801 tggttatatt atagagaatt tgctccctat atgaggcaag ggcacttaat tcttatatag
    22861 atgcttggaa gtacggcttc cgaacagggt aatctttcaa aaaagtacga tgagaaacac
    22921 aaagaaacta agacaatttg gaatattttt gcttattatt ttactatcta cctatttacc
    22981 acaaacaatt gaattgtatg tgacgatcat tttagggtta ggagctgatg tctattctct
    23041 tattttaaca atggtattag tgggaagttt ccttctatta atttggaggt taaaaaagaa
    23101 aaagagctgg aactggtcat ttgtcttctc cttatttgca acttatgtgg tttatcagat
    23161 tctcggtaat ttttgggcac gctatgctca tttgattaat cataggaata ttcatgatga
    23221 gtattttacc gtggttcttt caaatggaca accaaccttt ttatcgacta tcttatcttt
    23281 tgtcctgcct gtaatcatcg gtcctgtttt tgaggagact cttgatagag ggtattttat
    23341 gaataccttc tttccctagt ccaagtatta tttagatgtc atcttatcag gccttatctt
    23401 tgggcttagt catttgatac tatctcacag agatccaatt agtttattat attatagtct
    23461 gattggtttc ttttttgccc ttgtttatcg ttttaccgac aatctgagat taacgattct
    23521 ctgccatagt tttttttaac tttctcaatc atgcaaaacc tatctggatt tttgtttaca
    23581 attatatcta ttatcatttt tttagatagt gggaggcaaa gaaaaagtgt ttgatatcta
    23641 tcaaacactt tttcttttta ttgttcttct gttacaaact ggctgagcag tccattgata
    23701 aaacgggcag atttttgatc ggagaagtcc tttgcaagct cgatagcttc attaacagca
    23761 accagctgag gagtgtcaaa tgaagtgatt tcaaagactc ccaagcgaag gaggtttctc
    23821 tccacgagcg ttaagcgttc aatggtccaa cctgctttta gatgctgagt gatttgctta
    23881 tctagttctt cctttttagc ttgaacacca gaaacgaggt ctatcaaaaa ggctggaagt
    23941 tgtacatccg tatcttcacg atcatgagta taggcgaaac gacaagcagt ttcgacatcc
    24001 gtaccgaact caaggctcat gagagcttga aaagcgcatt tacggagttg gcgtctagat
    24061 tctaatagtg gactagtcat tgaggaagtc ctcgtcaaat agatctttca attctggttt
    24121 tggtgtttta tctgggacga tacctgcaac gtgaatattg atagcagcga gttctacatc
    24181 agccatatta cggacggcat ctttgacagc tttctggata gcaaccgcta ccttaggaac
    24241 ttttactccg tactcaaggt agagatagat atctgctgtg agttcttcgt ccacgttttt
    24301 aagataaacg ccacggccga gtgaaagttt tgaaagggta tcagacactg atctgtttga
    24361 aaaagagtga acaccctcta cctttgcagt agcgatagca atgatttttt caagtacacg
    24421 tggggcgata acgatttcgc caagttgttc ttcaattccc atagaatgac ctctttctag
    24481 agattaggca cgagaaacgt aagttccttc tgcagtgttg ataacgagtt tttgtcctgc
    24541 ttcgatgaag tctggaacgt ttacgacaag tccagtttcc atcgttgctg gtttaccaga
    24601 acctgtaaca gtagcacctt tgatagatgg ttgagtttca gcaactgtca actcaacagt
    24661 agtaggaacg gtgacaccga tcacttcagt tccgtagaat tggattttca catcagagtt
    24721 ttcaaggatg taaagcaatt cgttttcaac attgactaca gggatttcgt actggtcgta
    24781 agtttctgta ttcatgaagt atgctgtgtc atccattttg tacaagtatt gagctgggac
    24841 agtctcgata atagcttgtt caaatttttc ctctggacgg tagcttgtgt caaatgtaga
    24901 accagtacgg acatcacgca atttcatacg catgatcgtg tttcctttac ctggtttgtg
    24961 gtgactagct tccaaaacgc gaatcaattt gccgtcagct gtttcaaagg tcataccagc
    25021 ttttaattta cttgcttcaa tcatgttttt acctctttaa taaatattat tactcttcat
    25081 tttaccataa aatactctgg aaataaagcc aaaatagtta ttgggagttg gtagagaaga
    25141 aaaaaccaac cttagggctg gttttgttct gtctagtttt ctttcaactt cgccaattcc
    25201 tgtgcaagta gtttaagggc aacttgtggg ttggcttggc ctttggttgc cttcataagg
    25261 aatcctgtaa aggctttgtc ggcgttacgt ttgcctgact tgaagtcggc aacagcagct
    25321 tcgttatcgg caaagacttg gtggatgatt gggatcaaga tagctggatc tgaaatttga
    25381 accatacctg ctttttccac gtattcacgc gcgccaccgc catttttagc tagatggaca
    25441 aagactttct tggcaatctt agatgaaata gtaccgtctt cgatgatggt aatcatttca
    25501 accaagtttt ctggtgtcaa ttcgatttgt tccagtgttt taccttcagc attcaagaac
    25561 tgagcgactt ccccttggag ccagttagag acttgtttgg catcaccacc tagggcaaca
    25621 gctttttcaa agaagtcaga agtgacttta ttagcagtca actgactagc atcgtagtct
    25681 gataaaccaa ggtcagatac ataacgcgca cgacgttctt ttggaaactc tggcaactca
    25741 gtccgcattt cctcaatcca ctcgtcagaa atttcaaaga ggggtaggtc tggttctggg
    25801 aagtagcggt agtctgcagc cccttccttg acacgcatga gaatggttgc tttattcgct
    25861 tcatcgtaac ggcgtgtttc ttggcggatt tgaccacctg agcgaagaat ttcagcctgg
    25921 cgttggactt cgtattcaag acctttacga acgtttgaga aggagttgag gttcttcaat
    25981 tcagtcttgg taccgaattt ttcttgacca taagggcgaa gggagatgtt ggcatccaca
    26041 cgcatagaac cttcttccat cttaacgtca gaaatgccag cgtactggat aacttccttg
    26101 agggctgtca gataagcata ggcctcctct ggcgaacgca tatctgcctc agatacaatc
    26161 tcaatcaagg gaaccccttg gcggttgagg tcaacataag agtagccatc tgtaccatgg
    26221 gtgtttttac cagcgtcttc ctctaggtgg gcacgttcga taccgatttt cttggtcgta
    26281 ccgtcttcta gtttgacttc aatccagcca ttatatccga ttggttcatc aaattgagaa
    26341 atttggtagg ctttggggtt atcaggatag aagtagttct tgcggtcaaa gtgcatcttt
    26401 ttgtggatgt ccatgttgag ggcaagagca gccttgatac cggcatcaac aaccccttta
    26461 ttgagaactg gtagaactcc tgggaaagac cagtcaatca cgttagtgtt ggcattttgg
    26521 tcatttccaa agtgggcaga agtaggtgag aagatttttg aattggtgtt gagctctacg
    26581 tggacttcaa gtccgatgac tgtttcaaag ttcattagtt gtcacctcca aaaatcacgg
    26641 gttgttgttt gtggtagtct gttgttgctt caaaagcagc agcagcttgg taaatggttt
    26701 cctcagagta cttgggacca atcaattgga gtccgacagg tagaccttga gagaatccag
    26761 caggaatcga aattccaggc agtcctgcca agtttacagg tatggtcaat aggtcggcta
    26821 agtacatggc aactgggtca tggttgagag aatccaagtc ataggcaaca cttggagcag
    26881 ttggacccaa aatcaaatcg taatccgcga agactttttc gaaatcttga atgataagtg
    26941 tacggacctg tccagccttc ttatagtagg catcgtagta acctgatgaa agactgaaag
    27001 tacccagcat gatacggcgc ttcacttctt caccaaaacc ttggcttcgg ctgtttacat
    27061 agatttcatc aaggttggtt gcatcttctg cgcgatagcc gtaacggata ccgtcaaagc
    27121 gttgcaagtt tgatgaagct tctgatgaag cgatgatgta gtatacggca actccgtatt
    27181 tagagtgagg aaggctgact tcttcgacaa tagcacccaa tttttcaaag tgtttagcgg
    27241 catttagaat ggtttcctta acctctgggt taattccttc accgaggtat tccttaggca
    27301 aagcgatttt catacccttg atgtcttggc cgatttttga agtaaagtcg gcgatgcgga
    27361 caggagcaga agtagagtct ttagcatctt cgctggcaat agcgttgagc aagagggcat
    27421 tttccttaac agtaggagca aaaggtccaa tctggtctaa tgagctacca aaggcaatga
    27481 gaccgaaacg tgaaactgtt ccgtaggttg gtttgagacc aacgattccg ttgaaggcag
    27541 caggttggcg gatggaacca ccagtatcag aaccaagtga caagcgaact tgtcctgagg
    27601 ctacagctgc ggcagaacca cttgatgacc caccaggaac cttgctgtgg tcccaagcgt
    27661 ttttagttgc tccgtagtgt gaagtttcac ctgaaccacc catagcaaat tcgtccatgt
    27721 tggtctttcc aacgacaatc atgcccttgg tttttgcatt ggcaacagct gtcgcatcaa
    27781 agattggctc atagttgtag agcatttttg aggcagcagt tgtgagaata ccgtctgtag
    27841 agatgttatc cttaacagca agtggaattc ctgaaaggac attgtcagca tcaattccag
    27901 cttcatcaat ggctttagct tgaacaagag cttgctcctc agcgatggtg acaaatgaat
    27961 tgagggcttc ctcacgagac ttgatatttt caagtgttgc ttgggtcaat tctgttgcag
    28021 aaatttcctt agagacaagg agattgtgca actcttcaat agttttattg ttaaaagtca
    28081 ttaggcatct cctccatcgt ctaggatagc tggcaccttg atatagtagt tgtctttttc
    28141 aggtacgttt ttaaacaagc gatcacggtc tgttccttct tcggccacat cagggcggag
    28201 tacagtcttg cggtcagcca tagtcgtagt aggtgcgaca ccagttgtgt caacttcgcc
    28261 cagcaattca accatgtcaa caatcttaga caaggtggtc gcaaaggcag cagtttcttt
    28321 ttcagagaat cttaattttg aaagattggc aacgtgtgtt acctcttctt gcgtaatttt
    28381 catcttgcat cctttcgtga aatgatgatt tttagtctgt tctattttac catattttcc
    28441 tataaataag ggagggggaa gtgaaaagaa atagaaattg aaaaaaatgc taaaatccga
    28501 tataatggag tgttgaaagg aatggtaaaa tccaatgtaa aaatcattct cagctattga
    28561 aacaagaaaa atagagaatc aaagaaagag aacttatgaa tattcaagaa gaaattaaga
    28621 aacgtcgtac ctttgccatt atctcccacc cggacgcggg gaaaacaacc atcactgagc
    28681 agttactcta ctttgggggt gagattcgtg aggctggtac ggtaaaaggg aagaaaacag
    28741 ggacttttgc taaatctgac tggatggata tcgagaagca acgtgggatt tctgttactt
    28801 catctgttat gcaatttgac tacgacggca agcgcgtgaa tatcttagac acgccagggc
    28861 acgaggactt ctcagaagat acctatcgta ccttgatggc ggtggatgct gcggtcatgg
    28921 tcgtggactc tgccaagggg atcgaggctc aaaccaagaa attgtttgag gttgtgaaac
    28981 atcgtggcat tccagtcttt acctttatga acaagctgga ccgtgacggt cgtgagcctt
    29041 tggatctctt gcaagaattg gaagaaatct tgggcattgc tagctaccct atgaactggc
    29101 ctatcgggat ggggaaagcc tttgagggct tgtatgacct ctataaccaa cgtttagagc
    29161 tttacaaagg ggatgagcgt tttgccagtc tagaagacgg ggacaaactc tttggtagca
    29221 atcctttcta cgagcaagtc aaggatgaca ttgagctttt aaatgaagcc gggaatgagt
    29281 tttcagagga agctattctg gctggagaat tgacgcctgt ctttttcggt tcagccctga
    29341 caaactttgg tgtgcagacc ttccttgaaa ccttcctcaa gtttgctcca gaaccacatg
    29401 gtcacaagaa aacagacggt gaaattgtgg atccttatga caaggatttc tcaggatttg
    29461 tctttaaaat ccaagccaac atggaccctc gtcaccgtga ccgtattgcc tttgtccgta
    29521 tcgtatctgg cgaatttgag cgcggcatga gtgtcaatct ccctcgtact ggtaaggggg
    29581 ctaaactgtc taatgttact cagtttatgg cggaaagtcg tgagaatgtg atcaatgccg
    29641 tggcaggtga tattatcggg gtttacgata ccggtactta tcaagttggg gataccttga
    29701 cagttggtaa aaacaagttt gaatttgaac cactgccaac ctttactcct gaaattttca
    29761 tgaaagtttc tgctaagaat gttatgaagc aaaaatcctt ccacaagggg attgagcaat
    29821 tggtgcaaga aggagccgtt cagctttata agaattacca aacaggtgag tacatgctag
    29881 gcgctgtagg tcaactccag tttgaagtct ttaaacaccg catggaaggc gagtacaatg
    29941 cagaagtagt tatgaaccca atgggtaaaa agaccgttcg ttggatcaag cctgaggact
    30001 tggatgaacg gatgtcgtca agtcgcaata tcttagccaa agaccgtttt gaccaaccag
    30061 tctttctctt tgaaaatgac tttgccctcc gctggtttgc ggacaagtat ccagacgtag
    30121 agttagagga gaagatgtga ctcagtacca acaattggaa ctaaagttcc aaattgttgg
    30181 acgctagtcg ctatttggcg aactagctaa ctgcctcact aactacgttt ggcaaataga
    30241 atcgatttgc caaactccgt gtcgtaatat aataatcggt aaccgctatg gcggcttacc
    30301 taaacgcttc actaggaaaa atccacgaat ggtattgatt tgtggatttt tccgtcgtag
    30361 cagaaagtag gtagcctagc taactgcctt actaactacg tttggcaaat gaaatcgatt
    30421 tgtaaaactc cgtgtcgtaa tgtaaaaatt gtaaaccgta atgggacttc atctaaaatc
    30481 tccactggta tggttttacg aacgaatagg cgaaaataat ggttacgtag aaacatatga
    30541 tgggaataat atgatgggaa taaaaagaaa taagataaag agtgaaagaa gagaaaaaag
    30601 cgattgtctt aggtgcagat aatgcttata tggataaagt tgaaacaaca ataaaatctc
    30661 tttgtgttca tcattataat cttaagtttt atgtttttaa tgatgacctt ccgcgagaat
    30721 ggttccaatt gatggaaaaa agactagaga ctttgaatag tgagattgtg aatgttcaga
    30781 ttgacagtag tattctgaaa ggttatcgtc tcccctttga gggactttca tatgcggctt
    30841 tttttaggta ttttattcca aagtatgtaa gcgagtctcg tgtactgtat ttggattcag
    30901 atattgttgt aagaaaatcg atagatgagc tgtgggattt ggacttgacg gctataccct
    30961 tagctgcagt tagagatgat ttttatacac ataattttaa ttctggtgtt ctgttaatca
    31021 acaatggcat gtggagagca gaaaatatca cgcaagacct gatttaagac acaaataaaa
    31081 aatcttccaa ttcccttgca aaaaacaaga aagtgtgata acatagtacg gtatgtggtg
    31141 ctagcacatc cgctatatta gatctaatag gaggaaaaca caatggctaa agtatgttac
    31201 tttacaggtc gtaagactgt atcaggaaac aaccgttcac acgcgatgaa ccaaacaaaa
    31261 cgtgccgtaa aaccaaacct tcaaaaagtt actgttctta tcgatggtaa acctaaaaaa
    31321 gtttgggctt cagctcgtgc tttgaaatca ggtaaagttg aacgcgttta ataataaaag
    31381 caaaggagac cttagggtct tttttctttt attgtgggta taacatcgtt tgaaaaatag
    31441 agtaaatatc cgctgataca ttattctgct tttacacttg ggctgaaata tgataaaata
    31501 gagtatcaac tagttgaggt aaaaaggatg actgtaaaaa ttaatacaaa agatggtcaa
    31561 atcgaactaa cagatgaagt gattgcaacc gtcgtaggtg gagcagcaac tgagattttt
    31621 ggtgtggtcg gtatggctag taaaaatgcc ctcaaagata atttccaagc cctgctaggt
    31681 aaagaaaatt attctaaagg tgtcgtcgta aaggcagccg aagatggcag tattgcagtt
    31741 gatgtatata ctgtgttaag ctacggaaca aagattagtg aagtgtcaaa aaacattcaa
    31801 gagcgtgttg cctttagctt ggaaaaccag cttggaatta ctgctcagac tgtaaatgtc
    31861 tacattcaaa atatcaaagt tgtaggagaa taaccgtgtc aaaaattact actagcttat
    31921 ttcaagaaat ggtgcaggct gcatcaactc gcttgaataa gcaagctgaa tatgtcaatt
    31981 cattaaacgt ctttccagtt ccagatggag atactgggac aaatatggga atgaccattg
    32041 aaaatggtgc taaagaagtt gcagacaagc cagcttctac agttggagaa gtagcgagca
    32101 ttcttgccaa agggcttttg atgggtgcgc gtgggaactc aggagtgatt acgtctcagc
    32161 ttttccgtgg attttcccaa gctatcaagg ataaagacga gttaacaggt caagacttgg
    32221 ctctggcctt ccaatcaggt gtggaagttg cctataaggc agtgatgaaa cctgttgaag
    32281 gaacgatttt gacagtttct cgtggagctg ctatcggtgc taagaaaaaa gctgagcaaa
    32341 cagatgacac tgttgaagtc atgagcgcag ccttggaagg tgctaaaaca gctctagcta
    32401 aaacaccaga catgcttcca gtattgaaag aagttggcgt tgtggactca ggtggtcaag
    32461 gattggtctt catctacgaa ggtttccttt cagcccttac tggcgaatat attgcatctg
    32521 aggactttgt agcgactcct gccaacatga gtgagatgat caatgtagag catcataagt
    32581 ctgtagctgg tcacgtagcg actgaggaca tcacgtttgg ttactgtact gaaatcatgg
    32641 tagctcttaa gcaaggtcca acctatgcta aagattttga ctacgatgaa ttccgtaact
    32701 acttggatga gcttggagat tctctcctcg ttgttaacga tgatgaaatt gtcaaagtcc
    32761 atgttcatac agaagatcca ggacttgtta tgcaagaagg tctcaaatat ggtagcttgg
    32821 tcaaggtaaa agttgacaat atgcgtaacc aacacgaagc acaggttgag aaagaagcta
    32881 ctcaagttat caagtcggct gaagaaaaag agtatgcttt gattgctgtg gtggctggta
    32941 aaggtctagc agatatcttc tgttctcaag gcgtggatta tgttatcgaa ggcggtcaaa
    33001 ccatgaaccc ttcaacagaa gactttatca aggctgttga acaggttaat gcccgtaaca
    33061 tcatcttctt gccaaacaac aagaacatct tcatggcagc tcaatctgcg gcagaagttt
    33121 tggagcaacc agcggtagtg gtagaggctc gcactcttcc tcaaggtatg acaagtcttc
    33181 ttgcctttga tccaagcaag tccattgaag aaaaccaaga gcgtatgaca gctgctctta
    33241 gcgatgtcgt tagcggaagc gtcacaacag ccgtgcgtga tacaacgatc gatggcttag
    33301 aaatccatga aaacgataat ttaggtatgg tggatggaaa aattcttgtg tcaaaccctg
    33361 atatgcacca aacattgact gaaaccttga aacatatgtt ggatgaagac agtgaaatcg
    33421 taaccttcta tgtcggtgaa gacggaagcg aagaacttgc caatgaaatc gctcaagaaa
    33481 tcgtagaaga attcgaagac gttgaagtcg agattcacca aggtcaacaa cctgtttacc
    33541 catacctatt tagtgtggaa taagattaat gaaggactga gaaatcagtt cttttttctt
    33601 tttagtaaat gaaatcggta tctttttaat aaaaacaaaa ataacattca taaataaacg
    33661 ttaaaataga aaattcagaa aatttcttct tttatcttga
//
LOCUS       DAVKLW010000019        33510 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKLW010000019 DAVKLW010000000
VERSION     DAVKLW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.019"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(657..992)
                     /locus_tag="VW324_000867"
     CDS             complement(657..992)
                     /locus_tag="VW324_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726535.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1083..2393)
                     /gene="der"
                     /locus_tag="VW324_000868"
     CDS             complement(1083..2393)
                     /gene="der"
                     /locus_tag="VW324_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9726536.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2407..3120)
                     /locus_tag="VW324_000869"
     CDS             complement(2407..3120)
                     /locus_tag="VW324_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9726537.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3117..4013)
                     /gene="dnaI"
                     /locus_tag="VW324_000870"
     CDS             complement(3117..4013)
                     /gene="dnaI"
                     /locus_tag="VW324_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9726538.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(4014..5183)
                     /locus_tag="VW324_000871"
     CDS             complement(4014..5183)
                     /locus_tag="VW324_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9726539.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEYPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5184..5657)
                     /gene="nrdR"
                     /locus_tag="VW324_000872"
     CDS             complement(5184..5657)
                     /gene="nrdR"
                     /locus_tag="VW324_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9726540.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5797..6162
                     /locus_tag="VW324_000873"
     CDS             5797..6162
                     /locus_tag="VW324_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9726541.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6167..6862
                     /locus_tag="VW324_000874"
     CDS             6167..6862
                     /locus_tag="VW324_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726542.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6879..7646
                     /locus_tag="VW324_000875"
     CDS             6879..7646
                     /locus_tag="VW324_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726543.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7999..9198)
                     /locus_tag="VW324_000876"
     CDS             complement(7999..9198)
                     /locus_tag="VW324_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726544.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILNHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9195..10088)
                     /locus_tag="VW324_000877"
     CDS             complement(9195..10088)
                     /locus_tag="VW324_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726545.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10204..10284
                     /locus_tag="VW324_000878"
                     /pseudo
     CDS             10204..10284
                     /locus_tag="VW324_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10769..10982
                     /locus_tag="VW324_000879"
                     /pseudo
     CDS             10769..10982
                     /locus_tag="VW324_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11397..12014
                     /locus_tag="VW324_000880"
     CDS             11397..12014
                     /locus_tag="VW324_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9726546.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(12334..13221)
                     /locus_tag="VW324_000881"
     CDS             complement(12334..13221)
                     /locus_tag="VW324_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9726547.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(13370..15307)
                     /locus_tag="VW324_000882"
     CDS             complement(13370..15307)
                     /locus_tag="VW324_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEU9726548.1"
                     /translation="MDYSKVAAEVIGAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGDKVKVGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGDAVIEVKI"
     gene            15683..17137
                     /locus_tag="VW324_000883"
     CDS             15683..17137
                     /locus_tag="VW324_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9726549.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            17118..18083
                     /locus_tag="VW324_000884"
     CDS             17118..18083
                     /locus_tag="VW324_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9726550.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(18118..19392)
                     /locus_tag="VW324_000885"
     CDS             complement(18118..19392)
                     /locus_tag="VW324_000885"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9726551.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(19392..20564)
                     /locus_tag="VW324_000886"
     CDS             complement(19392..20564)
                     /locus_tag="VW324_000886"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9726552.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETKSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(20695..20943)
                     /locus_tag="VW324_000887"
     CDS             complement(20695..20943)
                     /locus_tag="VW324_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726553.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIDELSSN"
     gene            complement(21011..21637)
                     /locus_tag="VW324_000888"
                     /pseudo
     CDS             complement(21011..21637)
                     /locus_tag="VW324_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21699..22148)
                     /locus_tag="VW324_000889"
     CDS             complement(21699..22148)
                     /locus_tag="VW324_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9726554.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(22150..22908)
                     /locus_tag="VW324_000890"
     CDS             complement(22150..22908)
                     /locus_tag="VW324_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9726555.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(23023..25002)
                     /gene="pknB"
                     /locus_tag="VW324_000891"
     CDS             complement(23023..25002)
                     /gene="pknB"
                     /locus_tag="VW324_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9726556.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKAQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(24999..25739)
                     /locus_tag="VW324_000892"
     CDS             complement(24999..25739)
                     /locus_tag="VW324_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9726557.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIVDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(25754..27067)
                     /gene="rsmB"
                     /locus_tag="VW324_000893"
     CDS             complement(25754..27067)
                     /gene="rsmB"
                     /locus_tag="VW324_000893"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9726558.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(27060..27995)
                     /locus_tag="VW324_000894"
     CDS             complement(27060..27995)
                     /locus_tag="VW324_000894"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9726559.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(28008..30404)
                     /locus_tag="VW324_000895"
     CDS             complement(28008..30404)
                     /locus_tag="VW324_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9726560.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(30470..30784)
                     /locus_tag="VW324_000896"
     CDS             complement(30470..30784)
                     /locus_tag="VW324_000896"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9726561.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(30809..31435)
                     /gene="gmk"
                     /locus_tag="VW324_000897"
     CDS             complement(30809..31435)
                     /gene="gmk"
                     /locus_tag="VW324_000897"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9726562.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(31565..33178)
                     /locus_tag="VW324_000898"
     CDS             complement(31565..33178)
                     /locus_tag="VW324_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9726563.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         9474 a         7611 c         6427 g         9998 t
ORIGIN      
        1 tcaattaggg gatctaaagc attttatacc aatgttttat aagttgtatt gtattattcc
       61 atattcaatc cactatacaa gatgaatatt tctctcaagt ttcataatca aaataaaaat
      121 gactgtatct atagctccat aaaaaaatca tagatacagt cattttttag ttcctacacc
      181 ttcctttatg cacaccttta gacttcccta gttcccgtct caagtcaagc atttttttga
      241 aacagatata gtctaaccag tcactagcaa aagcgataag tagtaataat agccaaacac
      301 cccaagactt gatgtctgcc acatttctca agacgatatt gaacaacagg accgaaaaaa
      361 ctgtccaagc cagactaaaa agagcataga gggtgatgta aatccagtaa aaatagtaaa
      421 aaattgagaa aatcttacta tctctgtttg ccctttcaat ccagctgaaa aagtaaaaac
      481 agggtaataa gattaccatt ttaaacaagt gttccatcac aaacctcctc ttttaaattg
      541 ttttctttag tttcgttcta ttaaatgtta ttaccaatat aaaccttgga atagcgtgta
      601 ataaatgatt ttagaggtac cataagccac ctcctacaaa tagaaaccga tataaatcaa
      661 tgccttccac ccttagactt ccctagttcc tgtctcaagc gaaacatttc tttgaaacag
      721 gaataagtta accaattcat accaatagct agcagaataa aaagaaacca aatgccccat
      781 aacttgatat ctgtcacatt tctcaagacg gtattgaaaa acagaactga aacaactgtc
      841 caagcaaggc taaaaagaga atagaagggg atgtaaaacc agtaaaaata ataaaaaatt
      901 ggaaaaaact tactatttct gttggccttc tcaatccagt tatcaaaata aaagtacggt
      961 gctaaaagta agaatttaaa caaatgttcc atcaccgaca tcaccctcct tcttttgata
     1021 gcgttttcta ttattttatt atatcaaaaa aatccggaac tgtcattcca gattctactt
     1081 ttttatttgc gttttcttgc gatgagatga atcggtgttc cttcaaaaac aaaggccttg
     1141 cggatttgat tttccaagaa acgcaggtaa gaaaagtgca tgagttcttc ttcattgaca
     1201 aagatgacaa aggttggtgg tttggttgcc acttgggtcg catagaaaat cttgagacgt
     1261 tttcctttgt ctgtcggtgt tgggttgatg gcaatggcat ccatgatgac atcgttcaag
     1321 acagctgatg gaatacgtgt attttgactt tcgctgattt gcttaatcat ctcaggaagt
     1381 ttgtggagac gttgcttggt taaagctgat acaaagataa tcggtgcgta aggcaggtat
     1441 tggaactgct cacggatatc ttcttcccag tttttcatag tgtggttatc tttttcaagc
     1501 gtatcccact tgttgaccac gataatcatc cctttaccag cttcatgggc aaatcctgcg
     1561 atacgcttgt cgtactcacg aatgccttct tccgcattga tgaccatcaa gaccacatct
     1621 gaacggtcaa tagcacgcat ggcacgcata acagagtatt tctcagtatt ttcataaacc
     1681 ttaccagact tacgcatacc agccgtatca atcatggtaa actcttgacc atctgtatct
     1741 gtaaagtggg tatcaatggc atcacgagtt gttccagcaa caggactagc aataacacgg
     1801 tcttcgccca agatagcatt gatcaagctt gattttccaa cgtttggacg accaatcagg
     1861 ctaaacttaa tgacatctgg attttcttct tcatattcat ttggaaggtt ttccacaatc
     1921 gcatctagta catcccctgt accgatacca tggacagatg agataggcag tggttcaccc
     1981 aaaccgaggg catagaaatc atagatatca tttcgcatct cagggttgtc taccttgtta
     2041 actgcgagga taactggttt gtgggtctta taaagcttac gggctacgta ttcgtctgca
     2101 tcggtaattc cttccttacc ggacacgaca aagacgataa catctgcttc ttccatggca
     2161 atttctgcct ggtgcttgat ttgttccatg aaaggagcat caacatcatc aattcctcct
     2221 gtatcaatca tgctaaaaga acgattgagc cactcacccg ttgcataaat acggtcacgt
     2281 gtcactcctt cgacatcttc tacaatggag attcgctcac cagcgatccg attaaatagg
     2341 gttgatttcc caacattggg acgtcctaca atggcaatag ttggtagggc cataatttct
     2401 cactttctac aataatttct tctgttcaag attttttcta gttgagcttg gttcagcttg
     2461 accaaactgt tctgctagac gctgactcca gcttgtggtc gcacgcgccc cagcatagtc
     2521 agcctgaaca cggtcataag cttggattgc ctcagttgac tgttcttggt attcttcctc
     2581 aaagacaaca ttctctagtg gcagtctcgg tttcatatca tgatgttgat ttggcacacc
     2641 cagtgccatc ccaaagacag aataggtgta gtcaggtagg ttaaagagct ctgccacttc
     2701 ttcagacttg tatcgaacca aaccgataat cacaccacca tagcccaagc tttcagctgc
     2761 caacaaggca ttttgtccag caagagctgc atcgaccgaa ctaatcaaga gaccttccac
     2821 accttggggt tggaaggtgt cggtatgaag tcgggctccc ttttctgctc ggttcaaatc
     2881 tccgacaaag agaaggaaaa cagcagactg gcgaatggct tcttgaggta ccaattcata
     2941 caaggcatct ttcttctctt gacttcgtac cacaatcaca gagtaggatt ggaaattctt
     3001 ccaagatgat gccatctggg ctgctgtcaa aatctcattt aagtctactt ggggaatttc
     3061 ttgctcttta aacctgcgca ctgaagtatg agccttcatc aatttaatgg tttctgtcat
     3121 cgacggttta ctccttctaa acgagtctcc tcagccaaat aacggatgcg ttccatgacc
     3181 cgtctggctt cccaggtttc gtcatttcca tgtttcactt tcgcaaaatg cttctccaaa
     3241 tcttcaaagt tgaagttgga tgtgaaaaag gtcggtaaat tttcctgcat ccgatattgg
     3301 agaatgacct gcaggatttc gtcacgcacc caaacggttg attgctcggc gccaatatca
     3361 tctaaaatca ggacctcaga cagcttaatc tcatccacca aggtcttaac attgccatca
     3421 ctgatagcat ttttgacatc aatgacaaag ctaggatagt ggaggagagt tgatgaaaca
     3481 ccacgttttt ctgataaatc atgagctaag gccgccacca tgaaactttt acccacacca
     3541 aagtctccat ataagtaaag accttttcga atagctggat attgctccac gaaggctagt
     3601 agcttttcaa aaactggtaa gcgccccaaa tcatccaagt caacttgagc caaactagct
     3661 ttcttgagac tggctggtag attgattaac ttgagacggt tcttaatagc cgcttctttt
     3721 tcagccgcga ttagctcagg agtttcttca tatgaaacat ctgcatagcc atgattctta
     3781 accaaaatcg gcttgtagcc tttggcaata taatccgtat ccccacggag aaacttgtca
     3841 cgctcggtga tgtactgatt aaacttggag atactgcgat ttaattcctc tggagtcaag
     3901 gattcttgct ggataaaagc cgcaacatca gggtccttca tgattttctg gaccaaatct
     3961 tgataataaa aacggctggg ttgacgtttg agtacgtctc cgacactttc catctaatct
     4021 cctccttttt ctaatcgagc taatagttct tgcttcttac gttctagttc cagacgagtt
     4081 tcctcgctgg tttcattctt atattcagga ttactccatt taggaacatt ggtttttact
     4141 ggggcagtct gattctgttt ttgtgttttt gctttctgtc ctcgctcacg gatgcgcaag
     4201 actgcctttt ctgccgaatg aatcttttga taggcatagt cattggctac cttcatggca
     4261 tatttctcat tgatatttgc cgaatcgacc ttattaaagg tcaataaaag aatgatattg
     4321 atgacttcgt ccagcaagcc caagccagcc atctgttgca agagttctct ttctgtttgg
     4381 gtaatggttc ccttgcgtgt ttgcttgatt tctgccaaga actgcagggc agttttactt
     4441 ttggcttctt tgataatggt tgtttcctta agactaaagt cagaggaaac tggtttttga
     4501 gcaatttttt cacgcatgcg tttggttgaa ataacctgag aaacagctgt tgacttggcc
     4561 aattgatagg tttcaaacca agtccatttc ttctcctcgg caatagcaaa gaggtttaag
     4621 acatcggact gctcatccgc aaaacgaagt ccatctcgag ccatcagctg gcgaaaatgt
     4681 tccaagtcaa aatcattggc cactttcttc ttgagaccaa ggtcttcttg actgcctagt
     4741 tctgccaatt ctggaaagac ttgattgagt gagacaggta tttcttcacc atcagcactt
     4801 tcaactttca aatcctccac agcagagtcg ccaatctttt tctctaagag tctgcgataa
     4861 acaggatact ccaagaagtc ttgactagat agaggagcat ggagggctag ctgataaaca
     4921 tccccctttt gatagagggt caagagatta aaagcagata agattttcaa tgattttatc
     4981 agtctatcca tcccaaagtt gagatgattg aggatacttg aaaaaagata ttcctttcta
     5041 ccattatccc aaaaactgat tgtataaaga taaaggctca gtgcctcctg accgataatc
     5101 gggaggtagc actgtaccag agatgaggta tcttgcgaca cccgattatt ctttagataa
     5161 gaaaaacggt caattggctt catttatctt tcctttttct ttttagagga ctgggtgatt
     5221 tgttggagca agctctctaa ctcactgaca tccttaaaac tacgatagac actagcaaaa
     5281 cgtacatagg taatctcgtc caattcagcc aactcctcca tgacgagtga accaatgtcc
     5341 tcactttgaa tttcattttc atttcgacca cggagtttct gttcgatacg attgactacc
     5401 atgttgattt catcacttga cacaggacgt ttctgggctg agcggataat cccattaaag
     5461 attttatctc tggagaattg ttcccgtgtg ccatcttttt taacaaccac taaggttctt
     5521 tcttctactc gttcgtaggt tgtaaaacgg tgttggcact cgtcgcactc tcgtcttcta
     5581 cgaatggtgt tcccttcttc tgcttggcga ctatcgataa cacttgactt ggtagcccca
     5641 cattttggac aacgcatcct ttccctcctt atcgttttct tttcattata ccatttttta
     5701 agcgattccc aaaacaattc ttctttttgc ttgacaagtt ttttgttttg ttgtattatt
     5761 taattaagac aaaagggtaa gagaaaggag actaagatgt cctggacatt tgacaacaaa
     5821 aaacccatct atttacagat tatggagaaa atcaagcttc agattgtttc ccatacactg
     5881 gaacccaatc aacaacttcc aaccgtgagg gagctagcta gtgaggctgg tgtcaatccc
     5941 aataccatcc aaagagcctt atcagacctt gaacgagaag gatttgtcta cagcaagcga
     6001 acaactggac gatttgtgac taaggataag gagctaatcg ctcagtcacg caaacaatta
     6061 tcagaagaag aattggaaca cttcgtttcc tccatgaccc attttggcta tgaaaaagaa
     6121 gaactaccag gcgtagtcag tgattatatt aaaggagttt aagcctatgt cattactagc
     6181 atttgaaaat gtatccaaat catatggagc aacaccagcc cttgaaaatg tttctcttga
     6241 cattccagct ggaaaaattg tcggccttct tgggccaaac ggctcaggaa aaacaaccct
     6301 gattaaacta attaatggcc tcttacaacc agatcaagga cgtgtcctca tcaacgacat
     6361 ggacccaagc ccagcaacca aggccgttgt agcttatttg cctgatacga cctatctcaa
     6421 tgagcaaatg aaggtcaaag aagccctaac ctacttcaag accttctata aagatttcaa
     6481 tcttgaacgc gcccatcatc tacttgcaga cctgggcatt gatgaaaata gtcgtctcaa
     6541 gaaactatca aaaggaaaca aagaaaaggt tcaactgatt ttggttatga gccgtgatgc
     6601 tcgtctctat gttttggatg aacccattgg tggggtggat ccagcagccc gtgcttatat
     6661 cctcaatacc attatcaaca actactcacc aacttctacc gttttgattt ctacccactt
     6721 gatttctgat atcgagccaa tcttggatga aattgtcttc ctaaaagacg gaaaagtcgt
     6781 ccgtcaagga aatgtagatg atattcgcta cgagtcaggt gaatccattg accaactctt
     6841 ccgtcaggaa tttaaggcct aagcaaagga gattatttat gttttggaat ttagttcact
     6901 acgaatttaa aaatgttaac aagtggtatt tagccctcta cgcagccgtg ctagtccttt
     6961 ctgccctcat cggaatacag acacaaggct ttaaaaatct accttaccaa gaaagtcagg
     7021 ctactatgct actttttcta gctacagtct ttggtggctt gatgcttaca cttgcgattt
     7081 caaccatttt cttgattatt aaacgcttca aaggtagtgt ctacgaccga caaggctatc
     7141 tgactttgac cttgccagtt tctgaacacc atatcatcac agccaaacta atcggtgcct
     7201 ttatctggtc attgattagc accgctgtat tggctctaag tgctgttatt attctggctt
     7261 taacagctcc agaatggatt cctctttctt atgtgattac atttgtagaa acacatctcc
     7321 ctcagatctt tcttacaggt atatccttcc tactaaatac catttcagga atcctctgca
     7381 tctacctggc tatttccatt ggacagcttt tcaatgaata ccgtacagca ctcgctgttg
     7441 cagcctacat tggtatccaa atcgtcattg gatttattga acttttcttc aatcttagtt
     7501 ccaatttcta tgtcaattca ctggtaggac tcaatgacca tttctatatg ggagcaggta
     7561 tagccattgt taaagaactc atattcatag ctatctttta tctcggaacc tactacatct
     7621 tgagaaataa ggttaatttg ctttaaataa tttttaccta gatatgtaac atactcatag
     7681 aacaaaagag accaggcaaa aagtctttaa aattagaaaa cgcatagtat caagtgttaa
     7741 aaatccttga tattatgcgt tttttgtgag ctgacttatt tcctttcgct atatcgcaaa
     7801 atgaaataag aacggaacga tgggattttg gaattcaaat caatttataa gaatgtttta
     7861 gaagtaatat tatcctattc cagattcagt tcactataca attgagtttt caagcaacct
     7921 gtttacataa tgtgtacata attaggttcg tgattccacc cttttcacct ttaaaaacct
     7981 cgctttcgca aggctcttct atttataaga taaggcacgt ttaaaggttt tccaaatccc
     8041 taaatcatcc gtttgaagaa cgagactagc atacatgcgt ccgataaatc ctgttgctac
     8101 caccgcaaaa atcactgtaa tagcaagtga aatccatgct tctgctcccc ccgcatagcc
     8161 attaatcgtt cgaaacggca taaagaaggt cgaaataaag ggaatataag aaccaatctt
     8221 caagaggaga ttgtcaccag ctgcacctag agctgtcact ccaaaaaaac cacccataat
     8281 caaaatcatc aaaggcgaca aggctttccc tgagtcctca ggacgagaaa ccatagatcc
     8341 taggaaggct gccaagacta cgtacatgaa aagactgatc aaaataaaga gcaaggtatt
     8401 cagtgagata gcatctccca agtgattcaa aataccagac tgagccaaga atggcaaatc
     8461 tttaaagagc aaaacggcag ccagaccacc tacaacatag atcccaatat gcgttaaaat
     8521 cactagaaac agagccatca tccgcgcata gaaatagtga cttgccctta tgctagaaaa
     8581 aacgacttcc ataattttgg tgcctttttc actggcaact tcctgagctg ttacacccgc
     8641 ataggtaatc agaatcatat aaagaaagaa tcctaaggca cctgctgcaa ttgtttgaat
     8701 aaacttttta ttttccttgg cttcatcaat cttttctgtg aattgaattg tctgcgctaa
     8761 gcgtttttcc tgctcttgag acaaggaagc agttgaacga ttaagctgat tttgcagttc
     8821 attgagtgta cctgtaacct caaatttaat tccattttca agcgatgttt cgccatgata
     8881 aactgccttt agaacactat cttcttgatc aatggtcaaa taacctttta atttttcttc
     8941 tttaattgct tctttggcac ttgcttcgtc tttatagtcg aagttaacac catttacatt
     9001 cttcagtcct tctgctacag atggcactgt tgtcactact gccactttat tatttttagc
     9061 catagaagaa ccttggatat gcccaattcc tacagagatt cctaaaaaga ggaacggcga
     9121 aatcaccata aagaagaaac tccatgactc gacatgtcga agataggttt ccttgattac
     9181 aacccacata tttctcatac ttccactcct gattctagtt taaagatttc atcgatagtt
     9241 ggcgcttgtt ggtcaaatgt tgcgatatat tgaccttgag tcaagattgg gaagagttcc
     9301 cttccagcgc tctcatcctc caaaatcaat ttccaactgc cttgtttggt caagctcacc
     9361 tgtttgacat gaggaagatt ttccaattct tccttgcttc gttcacttga aacaaagaga
     9421 cgcgttttcc cgtattgatt gcggacatcc tgaactggtc cgtgcaagac cacacggcca
     9481 tctcggatca tcagaatatc gtcacaaagt tcctcaacat tggtcatgac atggtcagaa
     9541 aagataatgg ttgctccgcg ctctttttcc tgaaaaatga cttgtttgag caattctgta
     9601 ttaactgggt ccaatccact aaaaggctca tccaagataa tcaggtctgg ttcatgaatc
     9661 agagtaataa tgagctgaat cttctgctga tttccttttg acagactctt gattttatct
     9721 gtcagctttc ctttcacttc caacctcttc atccattgag ggagtttttc tttgacttct
     9781 ttggcatcca tgccttttag agtcgccaag tagcgaactt gttcaagaac tgtcaattta
     9841 ggcatgagac tgcgttcttc aggcagataa ccaatccgag cataggtctc ctgacgaata
     9901 tcctgaccat ccagaccgat ttctccctga tattctagga atttcaaaat actatggaaa
     9961 atcgttgttt ttccagcacc attttttccg actagtccca aaatacgacc tggtcgcgct
    10021 tgaaagtcaa taccaaacaa aacttgcttg gagccaaaac ttttctctag acttcttact
    10081 tctagcatct ttcacctccg aaatttcttg cactcattat actccttttt gatagccttt
    10141 acaatgtttt tgtccatttt tagaagacta ttgctgtgta aaatatggcc tggagcactt
    10201 ttatactcaa tgaaaagcaa agagcgaact aggaagctag ccgtaggctg ctcaaagtac
    10261 agctttgagg ttgcagataa aactgacgaa gtcagctcaa aacactgttt tgaggttgtg
    10321 gatagaactg acgaagtcag taactatatc tacggcaagg cgaagctaac gtggtttgaa
    10381 gagattttcg aagagtatta gtgataaatc cattatacag tagcaaactc aatttatacc
    10441 ttccgctcct caactgtcta tttttaatcc tgaattgtta tttgagtaac tcctttttcc
    10501 tcataaagtt ttcttcctct aaaacttctg gaaaaaagcg aatagtttca gacaacattt
    10561 ttataagaaa caagttcatc tgtcatttca agaaggagta atcctttatc tactaatgga
    10621 cggaacggaa ttcaaccgct tgtccgatat attttctaag gattatatag taaaatgaaa
    10681 taaaaacagg acaaatagat cgggacagtc aaatcgattt ctaacaatgt tttagaagta
    10741 gatgtatact attctagttt caatctgcta tatctattat gcacacccct ataggatcta
    10801 atgaaaatca caacaggctc attcatagat ggttacctca ggaaactaag aaaacgacta
    10861 caaaggaagt cgcattcatc gaaaagtgga ttaacaacta tcctaaaaaa tgcttgaact
    10921 acaagtcccc cagagaagac ttctggatga ctaacttgaa cttgaaattt agcaataatt
    10981 aattcaccat ctaactatat ttagtaatta tttcagaact gattaatatt aaaattaact
    11041 aacaattcaa aggattcata ctagccataa attacgtcca tcagagagag actcttacta
    11101 cttttatatt ttagtctttc tggcttcagg atacatataa actttaggga aaatgactat
    11161 tcgaaagcgc gaatgcctca aaattatctc agataagcta ttcgaaactt agaatgcttt
    11221 taaatttatg gaattgcgat tattcgaaac ctagaatgca tataaccttt agttgacaga
    11281 cctattctaa gtctcgaagg gctatttact ttctattcct tatcaaaaaa gactcattcc
    11341 ccctttctcc tccaaaatat ggtatagtag aaatatacta tctatgagga gtttacatgt
    11401 cacaggataa acaaatgaaa gctgtttctc cccttctgca gcgagttatc aatatctcat
    11461 cgattgtcgg tggggttggg agtttgattt tctgtatttg ggcttatcag gctgggattt
    11521 tacaatccaa ggaaaccctc tctgccttta tccagcaggc aggcatctgg ggtccacctc
    11581 tctttatctt tttacagatt ttacagactg tcgtccctat cattccaggg gccttgacct
    11641 cggtggctgg ggtctttatc tacgggcaca tcatcgggac tatctacaac tatatcggca
    11701 tcgtgattgg ctgtgccatt atcttttatc tggtgcgcct atacggagct gcctttgtcc
    11761 agtctgtcgt cagcaagcgc acctacgaca agtacatcgg ctggctagat aagggcaatc
    11821 gttttgaccg cttctttatt tttatgatga tttggcccat tagcccagct gactttctct
    11881 gtatgctggc tgccctgacc aagatgagtt tcaagcgcta catgactatc atcattctga
    11941 ccaaaccctt taccctcgtg gtttatacct acggtctgac ctatattatt gactttttct
    12001 ggcaaatgct ttgacacgta aaaaatccgt ttggtttccc aagtggattt ttaaagcgta
    12061 gattaactat agcttgatac taaatatact ttggtatgga aatcatgcat atttttcgat
    12121 agtgaggcga ggacttacct agccttcccg ccgtgataga aacacctgaa atctaatggt
    12181 ttcaggtatt cggaaacttt gagcctagtg tctcaaagtt taggtatgga atttcgaaga
    12241 aagtcgctac cgtccgtaat cacttaagga aaggctcaaa aatattgttt tcaaccacaa
    12301 aatccgtttg gtttcccaag cggattttgt gctttatttt gaaacttctt ttgcaagaac
    12361 aaagttccca agtgtggcag aaccatttcc tgcgactgct ggcgtcacga tatagtcacg
    12421 cacatctggt actggtaggt aaccattaag aagagatgta aatttctcac ggacacggtc
    12481 cagcatatgt tgttgagcca tgacccctcc accaaagaca atcacgtctg ggcggaaagt
    12541 cactgtcgca ttaaccgcag cttgagcgat atagtaggct tgaacatccc aaacagggtt
    12601 gttgagttca atagtttccc cacgtacacc tgtacgagct tccaaacttg gaccagctgc
    12661 ataaccttct agacatccct tatggaaagg acaaacaccc ttaaactctt tttcaatatc
    12721 cattgggtgt ctagcaacat aataatgacc catttcaggg tgacccacac caccgataaa
    12781 ctcaccacgt tggatgacgc ctgcaccgat acctgtaccg attgtgtagt aaaccaagtt
    12841 ttcgatacga ccaccagcat tgttacgggc aaccatttca ccgtaagcag agctgtttac
    12901 gtctgttgtg aagtacattg gcacgtttag ggcgcgacga agggcaccaa gcaagtccac
    12961 atttgcccag tttggttttg gagtcgtcgt gataaagcca taagtttttg agtttttgtc
    13021 aatatcaatc ggcccaaatg aaccaactgc aagaccagca aggttatcga attttgagaa
    13081 gaactcaatg gttttatcga ttgtttcgat tggagttgtt gttggaaatt gtgttttttc
    13141 tacaacgtta aagttttcat caccgacagc acagacaaac tttgtaccgc ccgcttccaa
    13201 gcttccatat aattttgtca tgataaacct cttgttttta ttttctttat tatagcatac
    13261 ttcgaaagtc taaatgtctc tattttttag attttcctct gtaaatctta ctatctaata
    13321 aaaacgaaca aacatgtcat ttgttcgttt tcacattaga gaggattgat tagattttca
    13381 cttcgatcac agcatccccc ttcgcaactg aacctgttgc gactggagct actgaagcgt
    13441 agtcagctgt atttgtaacg ataaccattg ttgtatcatc aagtccagct gcagcgattt
    13501 tgtttgagtc aaatgttcca agaacatcgc caactttcac cttatcacct tgagcaactt
    13561 ttgcttcaaa accgtcaccg ttcatagata cagtgtcaat accaacgtga atcaaaactt
    13621 cagcaccatt tcttgttttc aaaccaaaag cgtgccctgt tggaaaggca attgaaactt
    13681 cagcatcagc tggtgcatag accacgcctt ggcttggttt cacagcgata ccttgtccca
    13741 tagctccact tgagaagact gggtcattga catcagcaag agcgacaaca tcaccaacga
    13801 taggagttac aagtgtttca ttttgaagag tatcagtctc ttccactact ggtgcttcta
    13861 ctgtaggttc tgtaactgtt tctgcggttg ctgtggcagc ttcatcagca aagacactag
    13921 ctgcttttgt tttcccgtag aaataagtga aagcaaaggc ggcaacaaag gacataattt
    13981 ccgcaattat atacataggg attgaaccag ccttaataga aaggaatcca ataaatccag
    14041 ccgctcccaa tgaaactgca ataacgtgag ttaaacctgc tacaaaggca ccaattgctg
    14101 aaccagcaag ggcgcagaag aatgggaatt tgtatttcaa gtttacccca aagagagcag
    14161 gttctgtaat accaaggaag gcagaaacag cagccgaact ggacaatcct tttgttttag
    14221 cattttttgt caagaagtag actgcaagag tagccgcacc ttgtgctacg tttgccattg
    14281 aagcaacaac aaagatgtag tctccaaatc cagtaccgtt attattgtat gcagacaaca
    14341 attgtgtttc gattgctggg aatgattggt gaagacccgt cataacgatt aatgagtaag
    14401 ttccaccaaa tagccccata ccaaaggcac ctgttgtgtt gtacaaccat acaatcgcat
    14461 ttgtaatcgc atcagacaca accaacataa caggaccgat aactgtaaag gtaaggaatc
    14521 ctgtaatcat cactgataaa agcggtgtaa aagtgaagtc aactgcagat ggcaatttct
    14581 tgtggaagaa tttttctaaa ttagctaaca accagactgc aacaaggact ggaataacct
    14641 gataagcata gtttgtttgt gtaacatgga aacctaagat gtcccagaag gcttctttac
    14701 cacccaaatc tggagtggtc ataatcatac cgattgccgc accaaggaat tggtttgctc
    14761 caaagcgctt ggctgctgaa ataccaacca aaacagggag gaacataaat ggagcagcag
    14821 acatcaattg aatcatagct gagaagcctt tgatagcagg atacatatct tcaattgatt
    14881 gaggtccaaa caaatctggt gatgtcaaga agttccgtaa agccatcaag agaccacctg
    14941 caactaaggc agggataata ggaacaaaaa tgtctgacaa aagtttaatc aagtccatca
    15001 atggattgaa ctttttgtct ttattagcaa tctctttcaa atcatctgtc gaaacttctt
    15061 tcaaaccagt tttcttaata atttccgcat atacgaagtt aacatcacca ggaccaatga
    15121 taatttgata ttgtccgtta gtgctaaaag tccccttgac atccgcatta ttatccagag
    15181 ctgcttgatt cacttttgct tcgtctttca ataccaagcg aagacgtgtc gcacagtgag
    15241 ctgcagctac aagattatcc ttaccgacag ccccaatgac ctcggcagca actttactat
    15301 aatccatagc aaaaatctcc ttatataaca ttttgttctt gtttgaaagc gattttattc
    15361 gcccttacga ctattatttt atcatgtttt aaaaatatgt caagcgtttt gcagaaaaaa
    15421 tattctatcc acttagtggg caagttttag attgattgtt tttgactgtt tcatcaggaa
    15481 ttccttgaaa aataagtttg aaaccttggt taaaaagtct tttatttcat tatctttcac
    15541 attttcgtaa aaatttgatt gatttcctgt atttatttta tgataatcgt ttgacagttt
    15601 ttcctctttt ttaacataaa aggcttgcat tttttatcga aaacagttac tattaaaagt
    15661 gataagattt ttggaggaat gaatggaatg gacaactgag cgtcgttaca gactttatca
    15721 agattggacg caagaagaaa ttcaacatat aaaggaaaat atggcacaat ctccatggca
    15781 tactcattac catgttgagc caaaaacagg acttctcaac gacccaaatg gcttttctta
    15841 ctttgatggc aagtggatcc tcttttacca gaattttcct tttggtgcag cccacggttt
    15901 gaaatcttgg gcacagctag aaagtgatga tttggttcac tttaaagaaa ctggaatcaa
    15961 agttttacca gatactccat tagatagcca cggtgcctac tctggttctg ccatgcaatt
    16021 tggcgataac ttattcctat tttatacagg aaatgttcgc gatgaaaact ggatccgtca
    16081 cccataccag atcggtgctt tgatggacaa ggagggtaag attacaaaga ttgacaagat
    16141 cttgattgac cagccagcag actctactga ccacttccgc gatccacaaa tttttaactt
    16201 taaaggtcaa tactatgctg tcgtcggtgg acaggacttg gagaaaaaag gcttcgtccg
    16261 tctctacaag gctgtcaata acgactacac aaactggcaa gcagttggcg accttgactt
    16321 tgctaacgac cgtactgcct acatgatgga atgtcctaat ctggtctttg tagaggaaca
    16381 tcctgtcctt ctctactgtc cacaaggatt ggataagaaa gttctagact acgataatat
    16441 ctttccaaat atgtataaga ttggggcttc ctttgaccct aaaaatgcca aaatggtaga
    16501 tgtgtctcaa cttcaaaaca tggattacgg tttcgaagcc tatgcaactc aagccttcaa
    16561 cgctcctgat gggcgtgctc tagcagttag ctggcttggt ttgccagatg tttcttaccc
    16621 atctgaccgt tttgaccacc aaggaacctt ctctttggtc aaggaactca ctatcaaaga
    16681 cgacaagctc taccagtatc cagtcgctgc tattaaggac cttcgtgctt ctgaagaagc
    16741 cttctcaaac cgttcccaaa ccaagaacac ttacgaactt gaactcaact tggaagctaa
    16801 tagccagagc gagattgtct tacttgctga taaagaaggt aagggacttt caatcaactt
    16861 tgaccttgta aacggtcaag tgacagtgga tcgtagccag gctggagaac agtatgccca
    16921 agaatttggg acaactcgtt cttgccctat cgagaatcag gctactactg ctacaatctt
    16981 catcgataac tctgtctttg aaattttcat caataaagga gaaaaagtat tttctggtcg
    17041 tgtcttccca catgcggacc aaaatggtat cctgatcaaa tctggaaacc caactggaac
    17101 ttactatgaa ttagattatg gtcgcaaaac taactgatgt cgccaaactt gcaggcgtca
    17161 gtcctactac cgtttctcgg gttatcaata aaaaaggata tctatctgag aaaaccatcc
    17221 aaaaagtcaa tgaagccatg agagaattag gctataaacc caacaacctg gctcgtagtc
    17281 tgcaaggaaa atcagctaag ttaatcggct tgattttccc caatatttcc aatgttttct
    17341 atgcagaatt gattgataaa ttggaacacc aactcttcaa aaatggttac aagaccatca
    17401 tctgcaacag tgaacatgat tctgagaagg aacgcgaata catcgaaatg ttggaagcca
    17461 atcaggtaga cggcatcatt tctggtagtc acaacctagg aatcgaagac tacaatcgtg
    17521 tgacagcgcc gattatttcc tttgaccgaa acctatcgcc agacatccct gttgtctcct
    17581 ctgacaacta tgctggtggg gttcttgctg cccaaacctt ggtcaagaca ggtgcccagt
    17641 ctatcatcat gattacaggg aatgacaatt ccaattcgcc aaccggactg cgccacgctg
    17701 gttttgcatc cgtactccca aaagctccta ttatcaatgt ttccagtgac ttttctcccg
    17761 tcagaaaaga aatggaaatc aagaatatct tgacccggga aaaaccagat gccatttttg
    17821 cttcggatga tttgacagct attctggtca ttaaaatcgc tcaagaattg ggcatttctg
    17881 tcccaaaaga gctcaaggtc atcggctatg atgggaccta ctttatcgaa aattactacc
    17941 ctcaattggc tactatcaag caacctttgg aagagattgc ttgtctcact attgatcttc
    18001 tcttgcaaaa gattgaaggc aaggaagtcg ccacaactgg ttacttctta ccagttacgc
    18061 tattaccagg aaaaagtatt taaacacaag aaaactcaga ccgattcgtc tgagttttta
    18121 tgatcttaaa ttttcgagat agcgctgggc tgtctctagg ttaaaggttt tatctgagat
    18181 gaggcgctct actaggggag caacttcaga ttcactagcc ccagctagga gagctaggga
    18241 tttggcctgt agtttcatgt ggccttgctg gatgcccgta cttaccaagg ctttgagggc
    18301 tgcaaaattt tgagcaagac cgatggacac gataatctgg gctaattctc tggcagaagg
    18361 atttcctagt agatcatgac tgagagctac acgtgggttg aggccgatag agccaccctt
    18421 agtcgctaca ggcatgggca gggtcatctc accgaccaat tcttctcttt caaggtccag
    18481 cgtccagcag ctaagacctt gatagcgtcc atctcgactg gcaaaggcat gggccccagc
    18541 ttcgatggca cgccagtcat taccagtggc aatcaaaatc gcatcaatac cattaaaaat
    18601 tcctttatta tgagtagcag ctcggtaagg atcagcctgc gcaaactgac tagccaacgc
    18661 aattttctcc gcaatctctc gtccttgatc cttttggcgg ctcaagtagc gaaaggcgat
    18721 gcgacagctt gcagtcacca gagaatcagt cgcgtagttg gacaggattc ccatgagact
    18781 ctgtccctga ctgagttctt ctaagactgg tttcaaggct tccagcatgg tgttgagcat
    18841 attggcaccc atggcttcct gggtatcgac atgaatataa acaacgagaa agtctggttc
    18901 gccttttatc tgctcgacat gcagatcacg cgccccacct ccacgtttaa cgatagaagg
    18961 ataggcttga ttggcaagct ccaagagctc cgctttcttg ctggcaatct tctcttgcgc
    19021 tagtttagga ttagcaactt gataaagggc tacctgccca atcatctggc gctgatggac
    19081 ttgtgcagta aaaccacctg cacgcttgat gattttgctg gcatagctgg ccgccgcaac
    19141 cacagagggt tcttctgtca catagggaac ggtgtattcc tgaccgttga caagtacctc
    19201 cggaaccagc gaataaggca gagaaaaagt tcccaccaca ttctcactca gctggtctgc
    19261 cacagtcaca ctcatctgtt catccttctc cagactagct tgtctctcag gactaaggag
    19321 cgcctgagct tttagcagct cgaggcgctc ttggtatgat tttttagaaa atccattcca
    19381 acttatcttc attatttttc aaccttgcta taacggcgtt ggtggtcaag aatttcaacc
    19441 aaggcaaaat cttgattttc atagccagca aactgggcag attttgtttc atccaagttt
    19501 acttcctcaa aaaagacctt ttcatagtct gcaacggata gggcagttcg ttggttgagc
    19561 ttgttcaagc ggtctttatc caaataagct tcatatcctt caaccaattc accactgaag
    19621 aactcagcca cagctccact tccgtaacta taaagggcga ttttatcccc agctttcaag
    19681 ctatctgtat tttccaagag agacaaaagt ccaaggaaaa gtgaacctgt gtagatattc
    19741 cccacctttt gactgtagag aatagactgg tcaaaatgct tttgtaagag gtcttttttc
    19801 tcttgaggca ggctcttatc catgattttt ttcaagcctt ttagcgctaa tttaggataa
    19861 ggcaagtgga aacaaacagc cgcaaaatca tccagagtaa gctggtagcg tttttgatat
    19921 tcaagccaag tcgttttcaa actatccaag tattgttggg tagaataaac accatttaca
    19981 taaggagttg tcgagtaatt tggtcgccag aaatccatga tgtcacgggt ctgagctaca
    20041 ttgtcattat taaaggccat catgcgtgga ttttgtgtaa tcaacatagc tacacttcca
    20101 gcaccttgag ttggttctcc cggagtttca ataccatatt tggcaatatc actggcaatg
    20161 accaatacct tggactccgg agaattttcc acatgcaatt tggcataatg gagggcagct
    20221 gtcgccccat agcaggcttc tttaatctcg aaactacgag caaagggctg gatgcccagc
    20281 aagccatgca caaagacggc cgcagcctta ctctggtcaa ttcctgactc ggtagccaca
    20341 atgaccatgt caacttcttg tctttcttgc tcagttaaaa tagagtcact agcactggcc
    20401 gccaaggtca cgatgtcctc agttaggggc gcaatactca attccttgag taagagtcct
    20461 ttacttaatt tttcagggtc aattcccctc gcttctgcta agtcttgtaa tttcaagaca
    20521 tattgactgg tcgcaaaacc aatcttatca ataccgattg tcatatttac ctctgtttta
    20581 tcattcatgt aaaaaatcgt tctatactat tttatcacaa atggcagtaa aagagagaaa
    20641 aaagacttga ttcaccaaat caagcctctt attggtcatc attttaaaga atgattagtt
    20701 gctagagagt tcatcgatat aagtagcttt ataagctcca ttcacagtta tcagctcctg
    20761 gaggatcaaa tttcctgagt aagtccttcc catctcatct acaaattttt gataaaactg
    20821 actggtcgga atttctctga catccttatc aaatgtcttg tcaagtgttt tactaacctt
    20881 ctcagcaatc aattgatgct cttgccatcc actttgaaac tctgagcccg aactagaaac
    20941 catgactggg ataaacaaca aggtcagtag acttacagac aataaggaaa atagtagact
    21001 tcctgcaaaa ctagaatcct agttcatgat tgataatacc aacaatcaaa ttcattcgta
    21061 atccgaagcg tttacgatga tttcgatagg ttgttgaaat cattttaaac gtttttactt
    21121 tggcaaagat gttctcaacc ttgcttctct ccttagatag cgcatggtta caggctttat
    21181 cttcagctgt tagcggcttg agtttgctgg atttacgtgg agtttgtgct tgaggatata
    21241 tcttcatgag cccttgataa ccactgtcaa ccaagatttt accagcttgt ccgatatttc
    21301 tgcgactcat tttgaacaac ttcatatcat gacaatagtt cacagcgata tccaaagaaa
    21361 caattctccc ttgacttgtg acaatcgctt gaggttccat agaatgacat ttctatttta
    21421 ccagaataat tggctagttg atttttttag gacgatttat ttttacctct gttgcatcca
    21481 caatcaccgt attctcagca ctaagatgag tttttgaaat cgtaacacca ctttgaacaa
    21541 gagttacttc aacccattgg ctccgacgga ttaagttgct ttcgtggata ccaaaatcag
    21601 ccgtaatttc ttcataagtg cggtattctc gcacatatag aaagcgttgt taatttattt
    21661 atctcatttt tcagaaaatt cttttatttc tgtaaagtct acgatactcg atgtgttttt
    21721 atataatgat acagtctgag aatcactgtt ccgctagcca ttccaataga gattaccaga
    21781 gccaacatga caaccaaggt cgcacttgcc agtgctttat tatagtcccc tgtcacaaaa
    21841 aaggcagttg ttcggtagga gagataacct ggaaccagcg gtgccaaaat ggccaagata
    21901 aagaccacag caggtgtctt ataaagaata cttaaaatct ggctgacaca agaaccaata
    21961 atggctgcaa tgaaggtagc tacaatgaca ttggtcggtt ccttgagcaa gagatagatt
    22021 agccagacag tcatgcccaa aatccctcca ggtaagagca tagaccgttg cacattgagt
    22081 acgattaaaa aagtgataat ggcaagaaaa cttgctactg cttgtaataa aaaggttgtt
    22141 agtgtcatat tagttcatca ataccaaggc gacagaagtt cctgccccta aagcgagggt
    22201 aatgagcagg gattcaaaca tcttactcat accagagttt atgtggttgg tcataatatc
    22261 acggaccgca ttggtcaagg caatgcctgg tacaaacggc atgaccgcac cagctataat
    22321 caaatctgcc gttgaaggaa aacctgtgta gcgagcccaa aactgggcaa ttatcccaaa
    22381 gacaaaggct ccagcaaagg ctgtcacaaa gggaattcgg ataaattttt ccacatagag
    22441 ggaaaaggca aaaccaaata aggtcgccac tcctgcccca agtgcgtcgt agatatttcc
    22501 gctaaacata actgaaaaga aaggagcact aaaggtcgca gccagagtta cctgcaactt
    22561 agtataggga aggggttgag cttgcaaggc cgtcaattgc ttaaaggctg tttctaagtc
    22621 aatctgcccc ccaactagct gacgagaaat ctggttcaca tcgcagactt tttcgatgtt
    22681 ataagaagag gaggtcacgc gcttcatgcg cgaaatattg gtattttcaa tagagaaaaa
    22741 gatagcggca ggcatggcaa ggacattgca atccacaatc ccctgcgaat gcgcgattcg
    22801 aatcatggta tcttctacac gatggatttc tgagccactt ttaaggagaa tagtccccgc
    22861 tagcataatc acatcaatga cggcatttaa ttcttttgat tcttccatgc tttcctcctt
    22921 ttatcaactc cctctattct atcacaaatc tggactcaaa aaaaatcttt gccatgaaat
    22981 catgacaaag attgattact cattttgatt atccatctgc ttttaaggag tagctgaagt
    23041 tgttttaggt ttgtagattg aaatcttgac tctagtctta tttaggtcta ccttttcacc
    23101 tgctctagga ctttgttcaa caaccatgcc ttctacacta cctgcaggcg ctgtcgtcac
    23161 ttctacaact tctatattag cttccttaat cccaacaatt tgaatcaaat tgttcttagt
    23221 aaactccaag ctggaaccaa tgtaactcgg catggcaaca cttgtaactt ttttagctac
    23281 tgtcaagaca atttgagtag gtttactcac atcataagtc gttccggcac ctggactttg
    23341 tttcataatc gttcctggtt cgctttcgct ggactcttct tcctctatct taatcaaatt
    23401 ctcaggaacc ttcttctgct tgagttctga gattacttct gtagagttcc gtccaatata
    23461 gttccctaat tgaatcgtcg tagctttttt agctactgtc aaaacaattt gagttgcctt
    23521 gctcaagtca taggtcgtac cttctggtag actttgcttc aagaccgttc cagcctcact
    23581 ctcattcgac tcttcttcct caattttaat caaattatct ggaacttttt tctcttttaa
    23641 ttccgcgata acatcagaag atttccggcc gacataatta ctaatttgga aggattgttt
    23701 gcctgatgag acaaccagat tgattttcgt tccttctttt cgaccagttc cagcgccagg
    23761 atctgtacgg ataatccgcc cttcttccac cttttcacta gcctctgtct tctcctcacc
    23821 aatctcaaaa ttggcttttt tgagcgttgc cttggcctct gcaactgtct gacctgccac
    23881 atctggaatg gcaatggttg caggagttct ggatagtatc caaataagag aagctgccac
    23941 caatacaagg ctggccaaca aaatcaggta acgcatctta aatctatgtt ttttcggtgc
    24001 ttgtggttgg taagtttcct ctgtcacagc ctggcttggg tttttgattg atttgtgttc
    24061 tgtctgcgct tgagccttag gaatagatgt caaggtactc tgagaaacct tcggcaaggt
    24121 cttggtatct gccttgctcg tttcatcaaa gattaactta ctttcatttc tacgattgta
    24181 ggacaagcta ctagacaagt ccacatacat ctctgaaacc gagcggtagc gattggtcaa
    24241 ctttttagca gtcgccttga taataacatt ttctaaagcc tgaggtacag atggattttc
    24301 tgcaataacg gacggcaggg gtttctggaa atgctggagg gcaatggtca ccgcgctatc
    24361 cccgtcataa gggatatggc ctgtcaacat ctcatagaaa ataatcccca tggcatagat
    24421 atcactctgc acagtcgcct tcgaaccacg cgcctgctct ggtgacaagt aatgaactga
    24481 gcccaacatc gagttagtct gggtcagact tgtctctgca aaggctacag caatcccaaa
    24541 gtctgtgacc ttggcagtcc catctggtgt caaaaggata ttttgaggtt tcaagtccct
    24601 gtgaacaatt cctcgagtat gggccaagcg catagccaag agaatttgtc ccatgatacg
    24661 gactgcttct tcattagaaa gaggataatg ttccttgata tagcgtttga ggtctagtcc
    24721 agcaacatac tccattgcaa gatactgttg accgtcttcc tcaccaatat ctgttatccg
    24781 aacgatatga ggatggtcta gatctgccat agctctcgct tcacgctgaa aacgagctac
    24841 agctatcggg tccgtctggt agttggtcct cagaaccttc actgccactt cttccccatc
    24901 taagattaag tctttggcta ggtagacatc cgccatgcct cctcgaccaa tctgtttgac
    24961 aatccgatag cgtccggcaa aaatcttgcc gatttggatc attctgcatc ctcctcgttc
    25021 atagaaacaa gggcaaccgt aatgttgtct aaacctcctg cattgttagc aaaacgaaca
    25081 agtgtctccg ttttatctgc taaaggaata tcactggtta caatatcacg aatctcactg
    25141 cctgaaatca tgttggtcaa gccgtcacta ttgagcaaga gatagtcacc tgactcaagg
    25201 ataactgtcc caaaatcagg ctgaatttca tctttttgcc caatagactg ggtgataata
    25261 tttttttgcg gatgagcttc tgcctcttct ggtgtcaatt gaccagcctt gagcaattca
    25321 ttaaccaagg aatgatcgct cgtcaactga tggtattctt ctccacgaat caagccgata
    25381 cgcgaatcac caatatgagc atagatagcc tgattatcaa caatagcaag gacttccaaa
    25441 gtagttccca tgcctctgta agcttcatcc tgaccaagct ggtgaatctt ttgattttca
    25501 atttctaggt aatgggcgaa ccattcacgc acttcattga ctgtatcgat ctgggtatca
    25561 acccaagcta cacccaggtc tgtgaccgcc atttcactag cgatattccc tgcacgatga
    25621 cctcccatcc catcagctaa aataatcatg gtacgtccag ctctattgac atagtggttg
    25681 acatagtctt ggttatttgt tcgtttctga ccaacatctg ttaataatga aatttccatg
    25741 tgtcagttcc ttcctaatcc gatatcttgc gaaattgact gatgaagaat ccatcacttc
    25801 catacaattc aggtgtaatg aggatacagc cgtctttcat gatatcctta cattcatgtt
    25861 ctagttttac ctgctcgaac tcgggatgac tctctaaaaa ggccttaacg acttgaaaat
    25921 tctcctctga gacgatagtg caggtgctat aagttattat accacctttg cctagtattt
    25981 gacaaacact acctaatatt tctaactgaa tttcctgcaa ggacgcgaaa tctgccgttt
    26041 ctttattgta tttgatatct ggttttcggc gcaaaagacc gattcctgaa caaggagcat
    26101 ccaccaaaat cttatcaaag gaatcctggt caaaaaactc atgcaccttt ctggcatcca
    26161 atttttgagt ttgaacccga tctgcaactc ccagacgttg ggcattttct tgaattaaat
    26221 ccaacttgtg gtcgtacaag tccagagcag taacctgacc tgtcgtaaga taagaggcta
    26281 tatgggctgt tttcccacct ggagccgcac aggcatcaag cactcgctca tcaccttgta
    26341 aatcaagcgt cggagcaacc aactgactgg actcgtcttg gatggtaatg gctccatccg
    26401 caaacaaatt atgccctgca aaatgcccct gctccttaac cagaccagtg gttgctaaaa
    26461 gggaattatt cgcctccaac aaggcttgga tttcctcttt tcgacttagg tctgttacac
    26521 gaatactggc tttgtttcgc actaacaggc tttcaaagat ggcttttgct ctctcctctc
    26581 cgtattcttc cttgagtttg gcaactagcc aaactgggag agaataggca atggagtcac
    26641 gcttgttttt tcgcttgatg ctagcaatat ctggccagcc ttcacgcaag atacggcgaa
    26701 ggacagcgtt gaccaatttt tcactgcctt ttttacggag tttggccaat tccactgctt
    26761 cattgaccac agcatgatct ggaatcttgt ccaaatagcg gagttggtag gcactcatga
    26821 gaagaaggac atagagccag ctgtctaact ggtctctgtc ttcgataaag tgggataggt
    26881 accattccag agtcagttta cgggctaccg ttccatagac cagctcggtc actaagccct
    26941 tgtctgctgc caaaagttga cttcccttta gatgcttatt taaggcgata tttgagtatg
    27001 cttggttcac aaaaacatcc tctagcactg ctagagctaa acttctagcc gtttctactt
    27061 tagtcaccaa atcgttctcc tacagtcaat gtacgtccaa ctccgttgag gaaggaagca
    27121 atgtccatct taggcttacc agctggctgc acttgtttga gggatagagc cccttcagcc
    27181 gttgcgacaa tcaattcttt cttgccgata gagagaatct cacctggatt tccctgacct
    27241 tctactggta gggcttcata aatcttaaag cggtcgccct taaggaaagt atgggcaaca
    27301 ggccaggggt tcattccacg aatttggtta aagagttgac gattggtttt gttccagtcc
    27361 agtttttctt cctctggctt tatatttgga gagaaggtaa cctgactcgt atcctgcggt
    27421 tcaggtttga tatcaccagc aatataggca ggcagagtgt ccaaaagcaa atcacgacca
    27481 actagcgcca atttttcaaa caaggtgcca acattgtcct catctgtgat cggaatgctg
    27541 cgacgagaaa tcatatctcc tgcatccatt tccttaacca tttccatgat ggtcacacca
    27601 gcttcctcat ccccttgaat caaggcataa tggataggcg caccaccacg gtgtctagga
    27661 aggagggagg catgaacgtt gacagcaaag tccatgctat caaggagttt gcttgggaga
    27721 aactgcccaa aagcagcagt cacaattcca tctgctccta gcttcataag atcttccatc
    27781 tctggacttc cagataattt ttcaggttgg tagatagata gtcctgcttc cttggcagcc
    27841 tgcttgactg gggtttcttg gataactttt ttacgaccaa cagcacggtc tggctgggtc
    27901 acaacggcta gaatttcgta acggtcatct gtcaaaagtc cttttaagac tgttgctgaa
    27961 aagtcggggg tccccataaa gattagtttt gtcatatctt ctccttctta taaaaattgc
    28021 tgcggctcat ggtcaatact gagacggagc tcactatttt cccgttcttg agtcaaggcc
    28081 aggacctggt tgagggtcgg ccccagctca tcttctaaac ggtatttaat taaaatctgg
    28141 taatgataga ggttgtgggt acgggcaatc ggttttggcg ttggccccag aatgggactg
    28201 gtctctgaca agcctgaccg caaaatgttc atgacttcat aggcacgttt gaaaacctct
    28261 tcttctttct tgtgagaaag ggtaatacca atcgtgaaat agtaaggtgg atagccgagt
    28321 tgtcgtctga ttcccatttc ataggcataa aagccttcgt aatcttgatc cttggcaaat
    28381 cgaatagcat agtgctgcgg attgtaggac tgtatcaaga cttgacctgc tttttcagcc
    28441 cgacctgctc gacctgccac ctgagtcaag agctggaagg ttctctcaga agaacggaaa
    28501 tcaggcagat tcaaggccgt atccgcattt agaactccga ctagggtaac attgggaaaa
    28561 tccaaaccct ttgcaatcat ctgagtacca agtaaaatat ccgcttcccc tcgcccaaac
    28621 tggtcaagca aggcttggtg actgcctttc tttcgagtcg tatccacatc catcctcaaa
    28681 atgcgagctt ggggaaagag ttctgctagc tcatcataag ccttctgagt tcccgtccca
    28741 tagtaacgaa tactgcggct cttacagtta ggacagacct gaggaatatc cttcgagaaa
    28801 ccacaataat ggcagttcat agtcttggta tccatatgca aggtcagaga aatatcgcag
    28861 ttgggacaag tatccaccgt cccacactcc cgacacatga caaagctaga ataaccacgg
    28921 cgattgagca tgagaaccac ctgctctttt ttaaccagac ggtcttggat agcctctagc
    28981 aaaggaggcg taaagtttga cgtctcattt tgtccgatat agtctcgaaa gtcaatcact
    29041 tgaacctcag ggattgtagc caaaggattg gcacgttggg ttagacgtaa gtgttgatag
    29101 acgcctttgc cagcacgtgc ccggctctct aagctcggcg ttgcagatcc aagtaccaga
    29161 gttgcttgat tatactgagc ccgtaaaata gctacctctc tggcatggta acggggattg
    29221 ctgtcctgct tataagccgc ttcatgctct tcatcaataa tcatgacacc cagatttttc
    29281 agaggagcaa agatagcaga tctggcacca acaacaactt gggcatcgcc acgctccacc
    29341 ttgcgccatt catcatactt ttcgccattg gataatcctg agtgaagaat ggctaccttg
    29401 tccccaaaac gtgctataaa acgctcggtc atctgaggag tcaaggaaat ctcaggtacc
    29461 agcaaaatag ctgtcttacc cttatccagg gcaccttgga taatctgcaa gtaaacctcg
    29521 gtcttcccac ttcctgtaat cccttgaagt agaaagggag gttgagaact gccaatagaa
    29581 ctcacaaccg catcacgcgc ctgtctttgt tctggattta actccaaagg tctacttgct
    29641 tcaattcctt caaaataagc agccgagcgt tgaacttcct tttggactat ggtaacagca
    29701 ccttgatcca caaagaagtt gacttgctct cgcgagtagg actctaacaa gctagccaag
    29761 gaagcgctct ctggatgaga cagcagataa tctctcagtt ccaacttttt cttggcacgt
    29821 gtagaaatct caacaccttc taattgggca tggtcaacct cataccaaga ctgggtcttg
    29881 accttctttt gatcgactgc ctgatattcc agaccaagca ggccttttct agtcaaacgc
    29941 atcatttcag cttgcttggc aaggtctagt gaagaaaagg ctagcgaatc ttctgaacca
    30001 aacaggcgta ctcgttcttc ctgactcaag ccttccagag gatagagaat cttgtcatag
    30061 ctagaattca gaaaccctgg aagcatggcc ttgaggatag agattttgta ggagaagaca
    30121 gatttgcgta actcctcagc cagccagagt tgttctggcg tgagaacagg agaaaaatcc
    30181 agcacctctg caatatcttt taaatcttgc tccatctctt ctccatctga ttgggacttc
    30241 aaaccaagaa caatcccttg aatcaggcga ttacccttac caaaaggcac atgaacccgc
    30301 atcccaactt ccagcattcc ctcaaattcc tccggaatcc tgtaactata gggctggtcc
    30361 gtctgcatca agggcacatc tacgataatc ttagctaggg ccatcttctc acctcctcct
    30421 tgtcagtaca ttcttgcaat agaaaaaata agattgagtc cccccaacct taaatttttt
    30481 caccatcttc tttttcttta gcaatttgct ctttgatttt cttttcttct tcttctttgc
    30541 ggcgtttttc ttcttcgata cggcgacgca ctgcttcacg ttttccttct ggatctgggt
    30601 gaattgtaac gtttcctgat tcgatttctt ctaaagcgcg aagagttgat ttttcagact
    30661 tgaaaccttg agttgctggg gcacctgctt ccaattcgtg ggcacgtttt gcttccaaga
    30721 ttacgagtga atattttgaa ggaaccttgt cgagcaaggt atcaatagag ggttttaaca
    30781 tcatttgctt gtacctattt tctaaatttt atcgggtagt tggagatttt ggtaacatct
    30841 cctgatagtg accaattaca cgatccacac agaagtgttc tgcttcaatc acacatttga
    30901 cacgttcagc agctaggggc acctgatcgt tgacaatcgc ataatcatac tcacgcatga
    30961 gggcaatttc ttccttggcc ttttcgattc gttgggcaat cacttctgca ctatctgttc
    31021 cacgacctac caagcgatct tgcaattcat ccaaatctgg tggtgtcagg aagataaaga
    31081 cagcatctgg aacctttttc ttgacctgaa gagcaccctg aacttcaatt tcaaggaaaa
    31141 catcgattcc cttgtccaag gtttcattga cataggtcag aggagttcca tagtagttgc
    31201 cgacatattc tgcgtattcc aacatctgtc cttgacgaat cagctcttca aattcttcac
    31261 gagtacggaa gaaatagtca acaccgtcca cttctccagg acgttgtgcg cgtgtcgtca
    31321 tcgatacaga atattgaaat tggttttcag aactctcaaa aatctctctt ctaaccgttc
    31381 cttttccaac ccctgaagga ccagaaaaaa cgattagtaa gcctcggtct gccattgtgt
    31441 ctccttttag tcaatctgtg aaataacatt tctctagaat aatggcaaaa agccagatta
    31501 tcctttacag tctttctatc tagtgtaaca aaaaagcagt aatttttcaa ctgctctttc
    31561 ttatttattt agcataatct actgcacgaa gctcgcgaat cacggttacc ttgatatttc
    31621 ctggataatc gagattgttt tcaattttct tacgaacttt gtgagccaag attgtgactt
    31681 tgtcgtcctt gatttttcct ggattgacca tgatacgaat ttcacgtcct gcttgaaggg
    31741 caaagctagt ttgcactcct tcaaagccgt tagcaatttc ttccaaatca tggagacgct
    31801 tgatgtagct ttcaagagac tcactacgag cacctggacg ggctgcgctc aaggcatctg
    31861 ctgcagcgac gataactgct atcacgctct cagcttcaac atctccgtgg tgactagcaa
    31921 tcgtattcac cacaactggg ggttccttgt acttacgggc caattccata ccgatttcaa
    31981 cgtggctacc ttcaacctca tggtcaatgg ctttcccgat atcgtgaagg aatccagcac
    32041 gacgggcaag agccgcattt tcaccaagtt cgctcgccat gataccagcc aacttagcaa
    32101 cctcaatcga atggcgcaaa acattttgtc catatgaagt acggaactgc aaacgtccca
    32161 taatcttcat caagtctgga tgaaggtttg gcgcaccaat ttcataggca gcagcctcac
    32221 cgtattcacg aatcttattg tcaatctctt gacggttttt ctcaaccaac tcttcgatac
    32281 gagctggatg tatacgacca tctttgagca acatttccat agtcatacgg gcaatctcac
    32341 gacgaatcgg atcaaatcct gacaaggtca ccacttctgg tgtatcgtcg ataatcacat
    32401 cgacccctgt caaactttca aaggtacgaa tgttacgacc ttcacgacca ataatgcgtc
    32461 ccttcatagt atcgtctggc agatgaactg ttgagtttgt tgactccgct acatattcac
    32521 cagcgatacg ttgcatagct tgaaccaaga tgtccttggc cattttgtca gaacgttcct
    32581 tgacctcttg ctcagcttcg cgaatgcgac tggcaatctc cctggtcaag ttttcctctg
    32641 tctgagccaa gataatatct cgtgcttctg cctgagacag agcaccaata cgctctagtt
    32701 ctgcttcttt ttgtctttcg acttcctcta attgctcttc acgcgcatca aggtttttcg
    32761 ctctatcaga aatactttgt tctttttgtt caagtgtttg ttctttactc gtcaaattgt
    32821 cgtccttacg gtcaaggcta gtagctctct ctgtcaaacg actttcgatt tgtttgagtt
    32881 cttgacgttc tgatttgaat tcagcgtcca cttcttcacg gtattttctg gcttcttctt
    32941 tggcctccaa tagtgcttct tttttaagag acttgctttc acgtttagct tcattaacaa
    33001 gtaaatccgc ttcacgctca gcttgtccac gtaaattagt tgcttcttgt tcagcattta
    33061 aaagcatcaa ctccgcagct tcctgagatg atttcatctt agctgagatg ctgacatatc
    33121 caatgactaa accaatgatg acggcaaaaa cagcaatcgc aagcgacatg atttccatgt
    33181 ttttacctca ttttattgtt attccgaatg acatacattc ttttacattc taccataaaa
    33241 aagtgatttt cacaaaccta aaatagaata tgttttgggg aatttagtct gaattttagg
    33301 attacacttc cttggttttt taaggagata agtcagtgtc gctatcaaaa agttccaccg
    33361 aatttctaca actatggatt ttctattgtt gtgccaaata gtgttccact agattcaaac
    33421 attttgcttt caagtctttg aaaattcact ttaggaaatt acagcttatc ttcaagatca
    33481 aatttgtcat aaattaactt tttttattta
//
LOCUS       DAVKLW010000020        33472 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKLW010000020 DAVKLW010000000
VERSION     DAVKLW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.020"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..475
                     /locus_tag="VW324_000899"
     CDS             <1..475
                     /locus_tag="VW324_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9726564.1"
                     /translation="QFHRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMYLTNKE
                     ILNKLLSYSEDLKHHYQLYNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNA
                     LQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(540..2102)
                     /gene="guaA"
                     /locus_tag="VW324_000900"
     CDS             complement(540..2102)
                     /gene="guaA"
                     /locus_tag="VW324_000900"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9726565.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2244..2942
                     /locus_tag="VW324_000901"
     CDS             2244..2942
                     /locus_tag="VW324_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9726566.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2985..3881)
                     /locus_tag="VW324_000902"
     CDS             complement(2985..3881)
                     /locus_tag="VW324_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9726567.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(3890..4825)
                     /locus_tag="VW324_000903"
     CDS             complement(3890..4825)
                     /locus_tag="VW324_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9726568.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4822..5547)
                     /locus_tag="VW324_000904"
     CDS             complement(4822..5547)
                     /locus_tag="VW324_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9726569.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5631..6266)
                     /locus_tag="VW324_000905"
     CDS             complement(5631..6266)
                     /locus_tag="VW324_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9726570.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6268..7095)
                     /locus_tag="VW324_000906"
     CDS             complement(6268..7095)
                     /locus_tag="VW324_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9726571.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFESNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7117..7257)
                     /locus_tag="VW324_000907"
     CDS             complement(7117..7257)
                     /locus_tag="VW324_000907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726572.1"
                     /translation="MYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEMNEHVGLK
                     WY"
     gene            complement(7303..7452)
                     /locus_tag="VW324_000908"
     CDS             complement(7303..7452)
                     /locus_tag="VW324_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726573.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPT"
     gene            complement(7453..7984)
                     /locus_tag="VW324_000909"
                     /pseudo
     CDS             complement(7453..7984)
                     /locus_tag="VW324_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8627..9238
                     /locus_tag="VW324_000910"
     CDS             8627..9238
                     /locus_tag="VW324_000910"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9726574.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLKAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9283..10011
                     /gene="rlmB"
                     /locus_tag="VW324_000911"
     CDS             9283..10011
                     /gene="rlmB"
                     /locus_tag="VW324_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9726575.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(10050..10961)
                     /gene="trxB"
                     /locus_tag="VW324_000912"
     CDS             complement(10050..10961)
                     /gene="trxB"
                     /locus_tag="VW324_000912"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9726576.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(11027..11251)
                     /locus_tag="VW324_000913"
     CDS             complement(11027..11251)
                     /locus_tag="VW324_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9726577.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11329..12072)
                     /locus_tag="VW324_000914"
     CDS             complement(11329..12072)
                     /locus_tag="VW324_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9726578.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVVQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(12072..12872)
                     /locus_tag="VW324_000915"
     CDS             complement(12072..12872)
                     /locus_tag="VW324_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9726579.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSVGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13030..13386)
                     /locus_tag="VW324_000916"
     CDS             complement(13030..13386)
                     /locus_tag="VW324_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9726580.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNREAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13380..13910)
                     /locus_tag="VW324_000917"
     CDS             complement(13380..13910)
                     /locus_tag="VW324_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9726581.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(13910..14404)
                     /locus_tag="VW324_000918"
     CDS             complement(13910..14404)
                     /locus_tag="VW324_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726582.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14539..14985
                     /locus_tag="VW324_000919"
     CDS             14539..14985
                     /locus_tag="VW324_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9726583.1"
                     /translation="MKTTFSYPKWAEIPNIDPYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14982..15629
                     /locus_tag="VW324_000920"
     CDS             14982..15629
                     /locus_tag="VW324_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9726584.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     NQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15650..16231)
                     /gene="pdxT"
                     /locus_tag="VW324_000921"
     CDS             complement(15650..16231)
                     /gene="pdxT"
                     /locus_tag="VW324_000921"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9726585.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16232..17107)
                     /gene="pdxS"
                     /locus_tag="VW324_000922"
     CDS             complement(16232..17107)
                     /gene="pdxS"
                     /locus_tag="VW324_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9726586.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17259..18638)
                     /locus_tag="VW324_000923"
     CDS             complement(17259..18638)
                     /locus_tag="VW324_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9726587.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18932..19855
                     /locus_tag="VW324_000924"
     CDS             18932..19855
                     /locus_tag="VW324_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9726588.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19915..20520
                     /locus_tag="VW324_000925"
     CDS             19915..20520
                     /locus_tag="VW324_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9726589.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20539..21783
                     /locus_tag="VW324_000926"
     CDS             20539..21783
                     /locus_tag="VW324_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9726590.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(22006..22263)
                     /locus_tag="VW324_000927"
     CDS             complement(22006..22263)
                     /locus_tag="VW324_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9726591.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22305..24341)
                     /locus_tag="VW324_000928"
     CDS             complement(22305..24341)
                     /locus_tag="VW324_000928"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9726592.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24553..25470)
                     /gene="glyQ"
                     /locus_tag="VW324_000929"
     CDS             complement(24553..25470)
                     /gene="glyQ"
                     /locus_tag="VW324_000929"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9726593.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25663..26424)
                     /locus_tag="VW324_000930"
     CDS             complement(25663..26424)
                     /locus_tag="VW324_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9726594.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26697..27539)
                     /locus_tag="VW324_000931"
     CDS             complement(26697..27539)
                     /locus_tag="VW324_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9726595.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27653..29044)
                     /locus_tag="VW324_000932"
     CDS             complement(27653..29044)
                     /locus_tag="VW324_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9726596.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTPFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEHLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29282..29746)
                     /locus_tag="VW324_000933"
                     /pseudo
     CDS             complement(29282..29746)
                     /locus_tag="VW324_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29900..30877
                     /locus_tag="VW324_000934"
     CDS             29900..30877
                     /locus_tag="VW324_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9726597.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30874..31956
                     /locus_tag="VW324_000935"
     CDS             30874..31956
                     /locus_tag="VW324_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9726598.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(32020..33050)
                     /locus_tag="VW324_000936"
                     /pseudo
     CDS             complement(32020..33050)
                     /locus_tag="VW324_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9929 a         7466 c         6032 g        10045 t
ORIGIN      
        1 tcagtttcat cgaaagtctc atgaatacaa ggctatcaag cgctactgga aactcattca
       61 acaggatagc cgtaaactga gtgataagcg attttatcgt cctacttttc gtatgtactt
      121 aaccaataaa gagattttaa acaagctttt gagctattca gaagacttga aacaccacta
      181 tcagctctat aataaggaac cagagaaatt tttcggactc attgaggaca atctaaagca
      241 ggttcatcct ctttttcaga ctgtctttaa aacctttcta aaggacaaag agaaaatcgt
      301 caacgccctt caactacact attctaacgc caaattggaa gcgaccaata atctcatcaa
      361 acttatcaag cgcaatgcct ttggttttcg gaactttgaa aacttcaaaa aacggatttt
      421 tatcgctctg aatatcaaaa aagaaaggac aaaatttgtc ctttctcgag cttagctgac
      481 ttcaacccac tacagttgac aaagagcctg atttcaaagc attttttgtt agtttaaaat
      541 tattcccact caactgttgc aggtggttta cttgtaatat cgtagacgat acggttaaca
      601 tgatccactt catttacgat acgtactgag attttttgaa gtacttccca tggaattttg
      661 gcaaaatcag cagtcatacc atcgatagaa gtgatagcac ggattgcaat cgtgtagtca
      721 tacgtacgac cgtcacccat aacaccgact gaacgaacgc ctgtgttaac agtgaagtat
      781 tgccaaatat cgcggtcaag tccagcttta gcgatttctt cacgaagaat agcgtctgat
      841 tcacgaacgg tttcaagttt ctcttcagtg atttcaccca tgacacggat agcaagtcct
      901 ggtcctggga atggttggcg ccatacgata tggtctggca taccaagctc tgtaccaaga
      961 gcacgaactt catccttgta aagagtattg agtggttcaa tcaattcaaa ctgcatatct
     1021 tctggaagac cacccacgtt gtggtgtgac ttgatagttt gagctgtatc cgtaccagac
     1081 tcgataacat ctgtatataa agtaccttga gcaaggaatt tcacatcttt gagcttgctt
     1141 gcttcgtcat cgaatacata gacaaactcg ttaccgatga ttttacgttt ttgttcaggg
     1201 tcagaaacgc cagcaagttt gtcaaggaaa cgtttagcag cgtctgcttt gacaatattc
     1261 aaaccaaact taccaccgag catgtccata acttgatcag cttcgccttt acgaagaaga
     1321 ccgtggtcta cgaagataca aatcaattga tcgccaatcg ctttttggag aagaacccca
     1381 acgacagatg agtcaacacc acctgataga ccaagaagga cacgtttatc accgacggtt
     1441 tcacgaattt ttttgatctg catgtcaatg aaattatcca ttgaccagtc acctttagcc
     1501 ttacaaatgt taagggcaaa gttacgaagg atatcatttc cgtatacaga atgacgaact
     1561 tctgggtgga attggatacc gtaaatgtgt ttatctgggt tttcgatggc tgcgtatggg
     1621 cagtcagctg atgtacctgt acgaacaaag tcagcaggaa tctcagtaac cgcatcacca
     1681 tggctcatca aaacagtctg ttcatcaggt gttgattcaa aaagcgctga tggtgtgtga
     1741 gttagggttg attgaccgta ttcacgattt ccagcatcac ctgcaggaac aacttttcct
     1801 ccaagtttat gggtcaataa ctgcatacca taacagattc ccaaaattgg aattccgagt
     1861 tcgaagattt ctgggtcaat atcaaatgaa ccatcttcat atacagaatt tggaccacct
     1921 gatagaataa ttcctacagg attgacttca cgaacttcag cagctgaaat tttatggctt
     1981 tttagttctg aaaaaacacc aatctcacgg atacggcgtg aaatcagctg gttgtactgg
     2041 ctaccatagt ccaatacgat gattttttct acatcttgca aatcagttga aatgttgctc
     2101 atctttttcc tttctcaaaa gaaatatctt ttctcaatat tctatcacaa ataagggcga
     2161 attaccaact aaacaaggcg aagtttgact ttttctagtt tattggggta caatagagaa
     2221 aagatagaaa tgaagtgaaa aatatgctac ctgcttatat gaaaatccat gatcagataa
     2281 aaaaggatat tgacgagcac cgttgggcta ttggtgagag gcttcccagt gaaagagatt
     2341 tagctgagca gtttgcggtc agtcgcatga ccctccgcca agccgtatct ctattagtcg
     2401 aagaaggcgt cttagagcgc cgtgtaggaa gcggcacctt tgtttccagt actcgagtac
     2461 aagaaaagat gcgagggaca accagtttta ctgaaattgt caaatcccaa ggtaaagttc
     2521 cctctagcca gctcatttcc tacaaaaaaa ccattcccaa tgagcaagaa gttgccaagc
     2581 taggaatttt tccaacggac aatattatcc gaatggagcg ggtccgctat gccgaccaag
     2641 ttcccctagt ttatgaagtt gcttctattc ctgaaaaatt cattaaggac tttaaaaaag
     2701 aagaaatcac cagtcatttc ttccaaacct tgcaaaaaca tggctatcgt atcggcaaat
     2761 ctcaacagac catctatgct cgccttgcta aagaaaagat tgcccactat ttggaagttg
     2821 aaaaaggaca tgctattctt ggattgaccc aggtttccta cctagaagat ggtactgctt
     2881 ttgaatacgt aaaaagtcaa tatgtaggcg aacgctttga attttatctt gaaaataatt
     2941 agattaccac gcaaaaactc tctgtcacgg acaaagagtc ttttttattt ttctaagaat
     3001 aaatctttca aagaaatcaa agttacagca aacaatatca ttgccatacc aataaaatca
     3061 atgggataaa attgttcatt cattattaag aaggcaaaga aaaccgccga tattggctca
     3121 attgaagcca acaagcttga cttgaccggt cctatcagac tggctccttt aaggaaagct
     3181 gtataggcaa agacagtccc gataaggata atgcctgcaa acgcaaggag aaaatcaaga
     3241 ctagtcggga tatcggcctg tagaacccct gtaaaaggaa gggcgaccaa acctgctatg
     3301 accattccta caccaatgac caagctgctc ccccactttt taatcaaggc tatgggtaaa
     3361 atgatataca gagcataagt caaggcagaa aagagacccc agaacagacc agcaggtgtc
     3421 atggataact ggtccaactg cccatgtgtt gcgatcagga aggttcctcc gatggcgaat
     3481 atgatggaaa ctatctctcc cagtgtcggt gccaccctat ccttgataca gctataaatt
     3541 aaaattccga caggacaaac atactgaagc accgtcgctg ttcccgcatt ggtctcctga
     3601 atagcagaca gataggcgaa ttggttgaga aaaagaccaa tcagagcaaa aataagaaga
     3661 gacagcaaac tctttctatc ctttaaaaag accagtattt tatcctttgc agtagcataa
     3721 gccaagagca tgagaattcc accagcgatt aaaagacgca agttggtcaa gaccagagcc
     3781 gaaattccgt gtgccattag gtattggcca ctcgttcctg acaaccccca agcaatacca
     3841 gccacaactg ttagtaaagt cccttttaaa ctatttgaca tgtctctcct tactcttctt
     3901 tgcgaatcaa gttcatcact tgattgcggt ctaatatcca ttgagctctc tcgtcctttt
     3961 cataggtacg attcgatagg aaaatagtgg cttcttgctt ctgacgattc cacatgataa
     4021 aggtacctgt atagcccgta tggtctagcc aatctccttc caaattccat gctaaagaac
     4081 gttccttgtc atccaaagga gaaaaatttt gatttaagtc tcttgcaaaa tcatctgcta
     4141 aatagtgttc taaaaagatt tgtaaatcct ttatagtcga aaataaacca gcactcccag
     4201 catgtctacc caggagacga gccttgggat catgcactat gcctgcctct acacctctaa
     4261 ctgttggaac agcaagctca actggcccaa acttggtttc cgtcattccc caaggtttcc
     4321 agacttgatc ctttaaaatc acatccaaat cttgattaaa aattctttcc aaaataaagc
     4381 ccaacagcaa aaaatggaca tccgaataaa gaaaggctgg ctgacttcgt ctgttgagat
     4441 gaaacatcgc ttcctttaat tcaggggctg ttaaaagatc acgattagga ataaaaggat
     4501 caaggtctgt tgcatgagtc aagagctggc gaatagtgat gtctggataa tcactctcag
     4561 gtaaaaaatc tattaccagt ctatcaatat ctaattgacc tatttcccac aagaaggtac
     4621 aaactgtgcc aaccccaaca accttgctga cactagctag gtcataaact agtcctgcct
     4681 cagtctgcaa gccatgctct gggtcactct ggcctaaata gaactctgtc cattgattgt
     4741 ccttaaaata cgcaaaagag gctccgggat aaattcctgc ctcgatttgt tcttctattt
     4801 ttttaataat cttggtccac ttcatacttc ttcaaaccac aattcaacat tatttctatc
     4861 tttacaaagg aagaattttt cagacttagg aataaaatat tcagtagact caaacttctg
     4921 gcgaagactt gctatgtcta attcattgac caagaacttg agcatagata agtcccaggt
     4981 aaccgtattg tccacagtca aatcctgccc ctgagctgga ataaaatcaa gggatgcccc
     5041 aatttcagat gattccaaga aactttcgat atcagttggg agatgtaatt ccatagaaat
     5101 ctcaaattta cttaaagaaa ttgatgccaa atctgtttga aattcaggct tttctcctac
     5161 ttctactaga cttgctatgt catcttccgc atgaatcaaa atcaaatcat cttctggtga
     5221 gaaaatttca aaagcgtagc cattttgacc tttatataat cgatgaatcg aatctgtttt
     5281 agataagatt ccttcaattt ctaagggatt ttccaccttg acaatcaatc tagctagttt
     5341 ttttcttccc tctaccttac gagtacgcat actgggagct tcttctaaaa ccagcttttc
     5401 aagacccgtt tggtcaccta gtgacagaaa ggccgattct tctaacaagg ccttcattcc
     5461 aagggtttca atataaaatg tttcatttaa ttttctatta ttagctttta aagtaggaat
     5521 aatccgtaca atctgattta cattcataaa ttcctccgtc actttttatt ttaaaggatt
     5581 ttagaatttt ttacaagata ttatgctcaa atttaacatt atttagctat ttagtaaaga
     5641 tagtctttca aggattttga caaagcctga taaccagcaa tactgaggtg cagtccatca
     5701 gttgtatatt cttttttgag ttggcctgct tggtctgtca aacaatcaaa tactggcaca
     5761 aattccacct gcatataggc agatgcaagc tcttgatagg cttgattcca gttctgaatt
     5821 ttttcattcg agcggatata gactgcctgc tggtactcct ctccctcatt gacaggcaaa
     5881 atggaaagca atttaatctc tgtcaatgga taatcgcgag caacggattg aatgatagct
     5941 tcgagattat tgagagcctc attcacagga acatcctttc cgatatcatt tgtcccaatc
     6001 agaagaaaaa ttttatctac tgctccacca tatagatgag catcaaggtt ctctagtaac
     6061 agtcctgtct gatagccacg aattcctcga ttgacaatcg tctttgaagt cccaaatagc
     6121 tcctgtagag gataatactc gacaatggaa tccccaataa aaagaatgtt tggttctaca
     6181 acagaaatgt gatttaggtg acgatactta gtttgaattt tttcttgttc ctttaggagc
     6241 caattttcta ataactgtac tgccacttca ttctcctttt tctaaccagt tttccaagac
     6301 ttgataaact cctccttggc tgttggctgg tgctagagca gtcgccacag ctttggcttt
     6361 ctcatcagca ttttccatcg cataggcaat tccagccatt tcaagcattt caacatcatt
     6421 ttcactatca ccaaaagcca tgatttcttg ggatttcaag tcccagcgct tgagtaattc
     6481 ctccaagccc catgctttat gaatcccagc ttggaggata tcaatgcaac catagccact
     6541 ggatacagct cggacacggc catcaaagag agcattgatt tcttccaaaa ccgaactcaa
     6601 acgttcctca ccaacaacca tgctcatctt gagcacacca ccaaagaggt cagatgttaa
     6661 ttcatccaca aattgcatcc gttggtagaa tttttcaatc atttctggag tcataaaact
     6721 ttcaagatct gtaaaaatcg taccttcctt gacaaaatca cccttcatcc ccgttacaac
     6781 aaactggtcc tgacacgctc gacccttgaa atgagtcaaa gccttgttga caatggcgtc
     6841 atcccatgtc tgagcctgaa tcaattcatt gctttcaaaa atacgagcgc cattagcaac
     6901 aaccagaacc actcgatcca ccaagggact cagtagttgt ctcatgcggt gaatttcatt
     6961 gcccgtcgca atgacaaaac gaatgcccct ttgatccaac tgatctaaaa tcttttccaa
     7021 tcgtgggaga tcaagctgac ctctagcatc cagcaaggtc ccatccatat ctgtcgcaat
     7081 taccttaatc atcatttact ttcctctgga ttcaagctaa taccacttca atcccacatg
     7141 ttcattcatt tctttatagg ctgtatcaat tcgttcctta gtcgcttcat ctaactggtc
     7201 acgatgactg ccatcctcta taaaatcttc caaaatataa gtttggtaat gatacaaggg
     7261 atatccttct ggtgaaaagg ttccctttgg tgttcgattg acctaagtag gatgagcttt
     7321 agctgttccg atagttgttt ttccatcctt cttcttgatg gtgacatcca tgagaacacc
     7381 acgttcagtc cacttagcat tctcttcatc tcccatagat tcaatccgtt gattggaaat
     7441 gaagttcccc atttaataga caatgagctt cttatctcca tctttttcaa ccgtttcaga
     7501 tggttcaaca acgtgaggat gccctccaaa gataatatcc gctccccaat cgatcatctt
     7561 gtgataaaga gctttttgtt cttcagttgg ttccaatcga tactcaacac ccatctgaag
     7621 catgataatg gtgatatctg cttccttctc tgcccgttca atttcagcct tcatcttatc
     7681 ttcgtttaaa tctgaaagat aacgattata gtcttcctta gaaatatact gctcaattcc
     7741 attgaaacca taggaatagg ctaacaatgc aaccttgata gcattcactt ccttaatgac
     7801 cagcggagcc tgaacacgtg gttcgtgcgt ataaactccg attggagtga ttccagcttt
     7861 ctcaataata tcggccgttg aaataactcc ctcaagttga aataactccc tcaagttgcg
     7921 aatccaaaat atgattatga gctaaatcca gcacatgata acctgcctcc ttaatagcat
     7981 ccataacttc agcaggagca ttaaagagaa gataacccgc taaataatga tccttattaa
     8041 tgattccttc aaaatcacca atagctaaat ctgctgctta gtctccttgc ttgagccaag
     8101 gagtcacata ctcaaaattt tcatgaaagt cataggtacc gtcttctttt ttagctgaaa
     8161 agaaaagtcc atcgtggtag agcaaatccc cgtgggccat aattctggca gttttttcct
     8221 cttcctactc ctgagacttt tgcttagtcc cctcttgaga aatagtatct cgtttttgac
     8281 tagtcaaggg attccttgga agctttcaaa caacaagacc aagcccttca taaaacgaaa
     8341 agctaaacca ccctgatttc gattttatct tctttgtatc ttcattctcc ctactttctt
     8401 atgcaagcct tttcttttta ttatatcaca gataagtatt tctttcacaa ttgaattgaa
     8461 cttcccatct attttctata aatcctaaat gccataatgc tttcaattct tgtcattttg
     8521 tgatatcatg tagaggaaat gaactaatcc acagtggctt attccaagta taccacttgg
     8581 gctttggcag tagctaactg cactaaacat aatataagga gagaaaatgt ctgcaataga
     8641 acgtattaca aaagctgctc acttaattga tatgaacgat attatccgtg aagggaatcc
     8701 tactctacgc gcgattgctg aggaagtcac tttcccccta tctgaccagg aaatcatcct
     8761 aggcgaaaag atgatgcaat tccttaaaca ttcccaagat cctgtcatgg ctgaaaaaat
     8821 gggactccgc ggtggtgttg gactggctgc tccccagtta gatatctcaa aacgcattat
     8881 cgctgttttg gtacctaata ttgttgaaga aggcgaaact ccacaggaag cctacgattt
     8941 gaaagccatt atgtacaatc caaaaatcgt ctctcactct gttcaagatg ctgctcttgg
     9001 cgaaggagaa ggttgcctgt ctgttgaccg taacgtgcct ggctatgttg ttcgccatgc
     9061 ccgcgttact gttgactact ttgacaaaga tggagaaaaa caccgtatca aactcaaagg
     9121 ctacaactcc attgttgttc agcatgaaat tgaccacatt aacggtatca tgttttacga
     9181 tcgcatcaat gaaaaagacc catttgcagt taaagatggt ttactgattc ttgaataaag
     9241 aaaatcccgt tgcaagacgg ggttttgtgt tataatagag gcatgaaaac aaatgatatt
     9301 gtctatggtg tccacgccgt taccgaagcc ctccttgcaa atacaggaaa caaactctac
     9361 ctccaagaag atctccgagg taagaatgtt gagaaagtca aggaactagc tacagaaaag
     9421 aaggtatcca tttcttggac atcaaaaaaa tctctctctg agattactga aggtgctgtt
     9481 catcaaggtt ttgttctacg agtgtctgaa tttgcctata gcgagctaga ttacatcctt
     9541 gcaaaaacac gccaagaaga aaatccactt ctattgattc tagatggtct aaccgatccc
     9601 cataatctgg gttctatctt gcgaacagcc gatgcgacca atgtttcagg tgtcatcatt
     9661 cccaagcacc gtgctgtcgg agtaactcct gtcgttgcca aaacagccac aggtgctatt
     9721 gaacacgttc caattgcccg agtgaccaac ctcagtcaaa ccttagataa acttaaggat
     9781 gaaggtttct ggacctttgg aacggatatg aacggtactc cttgccacaa gtggaataca
     9841 aaagggaaaa tcgccctcat cattggaaat gaaggaaaag gtatctctag caacatcaaa
     9901 aaacaagtcg atgagatgat taccattccg atgaatggac atgttcaaag ccttaatgcc
     9961 agtgttgctg cggccattct catgtacgaa gttttccgaa atagactata aaaaagtttc
    10021 cagtcatctg attggaaact tttttatgat taactatgtt ctgtaatgaa tttataggct
    10081 tcttgaccag cgatagctcc atctccaacc gctgttgtta cttggcgaag gtctttcaag
    10141 cgaacatctc caactgcaaa gataccgtcg actgcagttt tcatgtggtt atctgtcaca
    10201 atccatcctg cctgatcttg gatattcaat tctttaacaa aatcgctaag agggtccaaa
    10261 ccaacataga taaagacacc accgaaggct tgttctgtca cttgacctgt tttcacattt
    10321 tcaaatacga ctgattctac tcggttttca cccttgattt cccttactac agaatcccag
    10381 ataaagctga ttttttcatt cgcaaaggcg cgatcttgta aaaccttttg ggcacgaagt
    10441 tggtcacgac ggtgaacaat ggtaacagtc ttagcaaaac gagtcaagaa gagggcttct
    10501 tcaacagctg aatctccacc accaactacc aataaatctt ggtcacggaa gaaagcacca
    10561 tcacacacag cacagtaaga aacaccacga ctgttcagtt cttcttctcc aggcactccc
    10621 aaaggacggt gtttagaacc agttgctacg ataactgtac gtgtttcata tgtttggtca
    10681 tcagtcatca ctttcttaaa atcaccatgg tcttcgacat tttcaacata accataaatg
    10741 tgctccacac caagattttc aagtggttca aacatctttt cagccaattc aggtccacta
    10801 atattagcgt atcctgggta attttcgata tcagatgtat tattcatctg accacctggc
    10861 agaccacctt caatcaaagc tacttttaga ttgcttcgag cagcatacaa ggccgcagtc
    10921 atccctgcag gtccagcacc gataataata gtatcgtaca tatagattcc ttctttcttg
    10981 ttgtaactat ctttattcta actctttctt gtcaatcacg caaggattat gccttgaaaa
    11041 caagaattaa actcataacc gcaaaggata atactgggac aaccaagact ccaatcataa
    11101 tcatatcata gagatgggct tggcgagcct tggctgtctt atcaactccc gacatggctc
    11161 tcagaccaat ccaaatccct aaaaaaatca gaacaaggat ggtggtcaag atcaaactct
    11221 cgaaatataa agaaaatagt tgcagtagca tgatttctct catttctatc ttttttaaag
    11281 agtaaactca gctagtccaa ctaactgagt tttcctttat ctattatatc aaatataagt
    11341 ccgtttgtaa ctagcgaaga attcttttgt ccgctcttct ttaggggtgt ggataatctc
    11401 atccggtgtt ccagactcga tgattttccc cttatctaag aagagaatct tatcagccac
    11461 ttggactaca aaggacatgt catgactaac caaaatcatg gtttgacctg acttagcagc
    11521 atctgcaata gacttttcta cttcaccaac caattctggg tcaagggctg aagttggttc
    11581 gtctaagagc aaaacatctg gtttcatagc aagcgcacgc gctagggcaa cccgttgctt
    11641 ctgtccacct gataaatggc gaggataatg gttttcacgg tccgaaagcc caaccttagc
    11701 caactcttcc ttggcaatct tagtcgcttc ttggtcagat aatttcttga caacaaccaa
    11761 gccttctttc acattatcaa gtgctgttcg gcgttcaaac aaattaaact gttggaaaac
    11821 catagacaac ttacgacgta gggcaaggat ttcttcttga gtgattttag aaaaatcaac
    11881 tgaaaaacca tcaatctgaa tagagccact gtcaggtgtt tcaagataat tgagactgcg
    11941 aagaaaggtt gattttccag ctcctgaaga accaatcaag gctacaactt cccctttttg
    12001 aatatccaag tccagatgat ccaagacagt ctgtcctgaa aaggatttgc ttaaatttga
    12061 aatcttaatc attaacgaag gtctcctttc acatctgttt gcactgtatc aggtgcagaa
    12121 atagccattt ttctctcgat gaaacgaccg aggctttcaa ttccgatatt gaccacccaa
    12181 taaacaaggg caacggagat gaagcgttca aaatagcgat aatcagctcc acctagaatc
    12241 tgagcttggg caaagacttc cacaacaccc acactaaaag ctagagatgt tcccttggtc
    12301 aaaccgatga gggaattaat caaggttgga gtagctacca ccgctgcatt aggaataatc
    12361 actcgtcgat aaacttgcgc tcgggtcata cccagactgc gtgccgcctc aatctcacca
    12421 ggattaactg agagaatggc tgcacgaatg gtttcactag cataagctgc ctcattaaag
    12481 gcaaaggcga caatcgcaaa agctgcagct ggaatcgcat tgatattgag accagttccc
    12541 cattgctgat tgagggcttt caaagccaaa gggattccgt agtaggtcaa catgagttgc
    12601 accaaaatcg gtgtcccttt taagaaacta acaaagaagg cctgcaaggg atataaaatc
    12661 ttgacacgat tgatcttcac aatggcaaaa agaagcgcca aaaccaagcc aaaaagggca
    12721 ccaccaattg tcaacataat tgttgttgga agttgttgga caattctagg gattccatca
    12781 aagaccgaac gtaggctaaa cagcttgcca tccggaatca attgcatcaa gttttggtac
    12841 caatctgatg ctaaaatcgt tgtaacattc ataaaaacat cctcttctga taatgattca
    12901 ttctaccatg cttctgccgt caatgaaatt atttgctacg gtcagataca gactttgtct
    12961 acctactagt ttatcatact ttgaaacagg gaagttatat attttatcta tcaaagagat
    13021 agataaaaac tatttcagtc ccagttcttc ataagatttt cgataaccga tttgcttaac
    13081 agttccattt tctactaaaa tgggccgttt taacaacata ccgtcacttg ctagcaactc
    13141 agccgcttct cggtttgaca aacttcctac cttatctttt agccctaatt cacggtattt
    13201 gatcccactg gtgttgaaaa attgcttcaa ttcaaatcct gaggtttcta gccaattcaa
    13261 aatgacttct tggctaggtg tttcttccac gatatggacg gctttatagt ccacacctaa
    13321 ttgatttaat tcttgttttg cttttttaca agttgaacat ttggggtatt cgataaattc
    13381 taacatgtgc ttctccttct attgtttcta tctttaaaat ctactccttc atgctccaag
    13441 agccaagctt tcttttccac tcctgcagca tagcctgtca gacgcttgcc tgctcccaac
    13501 acacgatgac aaggtactag gatagaccaa ggattgcgtc ccactgctcc accaattgct
    13561 tgagcagaag ccacttgcag gtcttgagca atttgtccat aggtcactgt ctgaccataa
    13621 ggaattccct gtaaatagga ccaaactctc ttttcaaaat tcgttccaat tggcgccaag
    13681 agcaagttgg ataaatcctg aggctcgcct ttaaagtaat catctaagca agcaataact
    13741 gggtctaaaa taggatgact aacaacttct tctatcgttt catctcctag tcccctctca
    13801 aaatgcttct gctcctgaac ccaaattcca tacaaataat ggtcatcagc tacaagtgat
    13861 agaataccaa ttggtgagga gtagagtatt tttacatact tcttttgcat tatttcttta
    13921 atttttgata ggccaagcgt tcgccatcta ctggcacctt accaacttgt ttaaaaccaa
    13981 gtttttcaaa aatatgttgc ataaccttgt tttcagcatg cgtatctgag cgaaaatcaa
    14041 gataatcaaa accttcaatc aagccctcta agaaggtttg agcaactcct tttccctgca
    14101 catctgctgc cacagcaata cggtgaaaga ctagatactc tgactctcca gcttgccagt
    14161 ttccctcata aatagcttca taggcctcct ctggactctt ggtcacagca gcataggcta
    14221 gcagttctcc ctcttccaag gctacgtagg cttgccctga gataatatca tcgataataa
    14281 catctgcatt tggatagcca ttttgccact ggtcactacc agcatctgct aaacattttt
    14341 tggcatcctc catcacctgc atgatggcat ctacttcatt tggaaaagct aaacgaattt
    14401 ccatcttttt tcctcatttc tgactagttt ctcccatcat agcataattt aagtatttct
    14461 acaagcagtt aaaacttgac tttgtgtttt tgttatttta taatctagtt atcaaaacta
    14521 tataaaaagg agttgaaaat gaaaactacc ttttcctacc caaaatgggc agaaattcca
    14581 aacattgacc cctatctgga ccaggttttg ctctatgtca atcaggtctg cgcccctatc
    14641 tctcctaata aagacaaggg cctaacagca tctatggtca acaattatgt gaaaaatggt
    14701 tacctgacaa agcctgacaa aaaaaaatac caacgccaac agattgcccg tttgattgct
    14761 atcacaaccc tcaagtctgt attttctatt caagaaatag cccagacact taatactcta
    14821 caaactcaag caagttcaga ccaactctac gatgcttttg tggactacat gaaccaagga
    14881 attgacccag ctaaccctat tatccaaacc agctgccaaa ccgttaaact ctatcatcaa
    14941 actctagact taatcgatca tactcaagag gaggtaatcc aatgaacact agtcttaaac
    15001 tcagcaaaca actcagtttt ggagaggaga ttgctaatag cgtgacccat gctgtgggtg
    15061 cagtcatcat gcttatcttg ctgcctattt catccatcta tagttatgaa gcacacggat
    15121 ttttatcctc tatcggcgtt tccattttcg tcatcagtct ctttctcatg ttcctatcat
    15181 ccaccattta tcactctatg gcctatggtt cgacccacaa atatgttttg cgaatcattg
    15241 accattctat gatttacgtt gccattgccg gctcatacac gcccgttgtc ttgaccttga
    15301 tgaataactg gtttggctat ctgattattg tcatccaatg gggaacgacc atctttggta
    15361 ttctctataa aatctttgct aaaaaggtca atgagaaatt tagccttgct ctttacctga
    15421 ttatgggctg gttggttctg gctatcattc ctgccattat caatcaaacg acacccgttt
    15481 tctggagtct catggtaact ggcggactct gttatacagt tggagctgga ttttatgcca
    15541 agaaaaaacc ttatttccac atgatttggc atctctttat cctagctgcg tccgcacttc
    15601 aatacatcgc tattgtttat tacatgtaaa aaagttgaga aattcaatct caactttttt
    15661 ctttacacat attgataaag tactggtgca agcgcacatc atcagtcaat tctggatgaa
    15721 aagaacttac caacatattt ttttcttggg ctgcaacaat ttgattgttc actgttgcta
    15781 gaatttctac accctcacca acactactga taatcggacc acggataaag gtcattggaa
    15841 tcttgccaac tcccttacat tctgcttccg tgtagaaact tcctaattgg cgcccataag
    15901 cattacgctc gaccaccata tccatagttc ctagatgact ctctttctga gaagtgattt
    15961 ccttagccag caaaattaag cccgcacagg tcccaaacac tggtaagcca gatagaatgg
    16021 cttctcggat gggaagtagc atgttctggt cacgtaagag cttgcccatg gttgtagact
    16081 caccaccagg caaaatcaaa cccgacaagt cactctgatc ttgctgaaaa tcatctagat
    16141 ttctgagttc tacactctcg acacctaatt gatctagcac ttttgcatgt tctgcaaagg
    16201 ccccttgcaa ggccaatatt ccgattttca tctattttcc tcgttcagcc atgagaattt
    16261 ggatttcatt ttcattaata ccaaccatgg cttctcctaa atcttcagag atttgagcta
    16321 ggatttgagg attacggaag ttagtcacag ccttaacaat ggcactcgct cgtttaacag
    16381 gatctcctga cttgaaaata cctgaaccga caaagacccc ctctgcccct aattgcatca
    16441 ttaacgcagc atctgctggc gttgcaacac ctccagcagc gaaatttaca actggcaatt
    16501 ttccatgttc atgaacatat tggaccaatt ctacagggac ttgcaaatcc ttggcagcaa
    16561 cataaagctc gtcctcacgt aagttttgaa tgcggcgaat ttcctgattc atcatacgca
    16621 tatgacgaac agcttggacg atatcccctg tccctggttc tcctttggta cgaatcatgg
    16681 aagcaccttc agcgatacga cgcaaggctt cacccaaatc cttagcacca cagacaaaag
    16741 gaacttggaa ttctttcttg tccacatgga aacggtcatc agctggagat agaacttcac
    16801 tctcgtcgat ataatcaatt tcaatagcct ctaaaatctg agcttcaaca aaatgcccga
    16861 ttctgacctt agccattact ggaatactaa ccgcttcttg gatttcctta atcatctttg
    16921 ggtcgctcat gcgggaaact cctccagctg cacgaatatc agccggaatt cgttccaagg
    16981 ccatcacagc tgccgcacca gcagcttctg cgatacgagc ctgttcagga ttctgcacat
    17041 ccataataac accacccttg agcatctgtg ccaagttttt atttagttca taacgatttt
    17101 cagtcatttg ttttatcctc atcatttgta ttggtagcta ccttttcatt ttaagtcaga
    17161 acagaatgaa gcacaagata aaaaaactat aattgtatgg ggacagattg gctaaaaatt
    17221 atctgacctt aacttaaggc cagatagctc attcatttct atttttcagc cgtaagggca
    17281 gccattgtga tgtagttgta tggtttgttg aagtgtggca agaagaagag gtctgtcaat
    17341 gccaatttat caattgtcac atgctcttgg atagcaagtg agaacatgtg gattcccatg
    17401 ctaattgcaa tatcatgtga aaccatttgg gcaccaagaa tttcacggct atctttgtca
    17461 aagacaatct taattgctac ttcatggttg tcatgtttca tgaattctgg tttttgaaga
    17521 tcgttaaagc ctgtttcagt tgcgttgtaa ccagcagctt tcgctttttc aagagtcaaa
    17581 ccagttgaaa ccatgtgaag accgtagatt gagataccat ttgaaccttg aacaccgatt
    17641 ccttccaatt catgtccaca agcattgtag gcaccaacga taccagtgcg aacagcattt
    17701 gaagcaagag cgatatagct tgtatcttta cgagcattgt cataaacagt cgcacagtca
    17761 ccaacagcgt aaacgcctgg gatagatgtt tcttgtttct tgtctacaag gaaggcaccg
    17821 ttgcggaaga gttcgatctt accatctgca agggctgtgt ttggacggaa accaactgca
    17881 aggataacca tatccacgtc aaagctttct ttgtcagtaa tcaagcgttc aactttaccg
    17941 tcaccttcga ttgctttaac agtttgacct agagccaagc ggatgttgtg atcttccaag
    18001 ttcttcgcca tcatttgtgt gaagtctttg tcatagtaac cgttcaagac agtatcaacg
    18061 atatcaacaa ggacaacttc ttttccaaga cgttcaaagg cttcagcaag ttcaacaccg
    18121 atgtaaccac caccaacaac ggcgatacgg tcgaggtgtt ggctcttgtc agaaagttta
    18181 ttgataactt cttcagcatt ttggtacaat ttcacgaatt gtacgttttc aagagttgct
    18241 ttaaattcgc ggtttccttt aacaatttca acaccttcga ttggtggcaa gattggtgta
    18301 gaacctgtag cgaaaatcaa tttttcgtat gattctttgt gctcttttcc ttcaacttcc
    18361 gctgtaacta ctttgttatc atagtcgatt gaaagaacag gtgagttcat gtaaacttta
    18421 gcacctttag cttccaattt ttctttatca gaatagaaca agccttcagc accgtcaatt
    18481 tgttcaccaa tccaaagagc cattccacat cctaggaaag agatgttaga gttttggtca
    18541 aatacaacaa tttcgttctc atttccaaaa ttatccaaca tggtattgat acatgctgta
    18601 ccagcgtggt tagcaccgac tacaacgatt ttactcataa aaatttcctg ccttcattct
    18661 aaatttacct tactagtata ccatttaccg ttagcgttta caaggggggt actattattt
    18721 cagcattttt tatcaaaaat aattccccat ttatcctgtt ttcaagcctg atgaatttta
    18781 tgagcatatt tcttgacttt ttccaaattt tgtttgtgaa aattctaact ttatggtatt
    18841 ataataacat gaaaatgaaa tgtaagggct tcaatttaga tgattatttg atattacaac
    18901 ttattttcta taagagaaag gagttggtag cttgactctt cgttcacatt ctgaacggct
    18961 aatggggact actatcacta tttcattagt ggatgagcaa gccgatatct ttctccaaaa
    19021 atcctttgac ttgctcaaag aacttgaata ccgcttcaac gccaatagtc aagaatctga
    19081 gttgatggaa atcaattatc aagcaggaat agcccccgtc acggttcatc cagacctgtt
    19141 tgagctgatt tcacttgggt tagagcatag cctagcgctc tctagccacc tcaatatcag
    19201 cattggtccc ttaattcaaa cctggcgtat cggtttttca gatgccaagg tcgcgcagcc
    19261 tcaagaaatt gaatcggtgc tgcctctaat caatcctcat ggtatcgagt tagattcttc
    19321 tacttctact gtgtttttaa aacagaaagg aatgaagatc gatctaggtt gtttagccaa
    19381 aggatacagt gcggataagg ttgcccaatt tcttaggaaa gagggggtga cttctgcctt
    19441 gatcaatctg ggagggaata tcctgaccat tggaaaaaat caggcaagag gggataaccc
    19501 atggcaaatc gggattcaag acccagccaa tcctagggga aatcatttaa tgaccatccc
    19561 tgttgtcaat aaatctgtcg tgacttcagg catttatgaa cgtcacctga ccgtcgatgg
    19621 acaagattac catcacattt ttgacagtca aacaggatat cctgttgaaa cggaactagc
    19681 gagtctaaca atcatctctg ataaatcagt cgatggcgaa atctggacga ctcgactctt
    19741 tggagaaaga cctgcttcta tcctctggca agtcgaaagt ttggagggca tcgaagtcat
    19801 cctcatcgat aaagaaggcc acctaagctg ttcttcagga attcctactc tatagagaca
    19861 actgtttcaa tggaatttta aaattgtatt atgtaaaaag agaaaggaaa tctcatgtta
    19921 aaacttattg ctattgttgg aacaaattca aaacgttcta caaaccgtca attgcttcaa
    19981 tacatgcaaa aacactttgc tgacaaagct gaaattgaac ttgttgaaat caagaccatt
    20041 cctgtcttca acaaaccagc tgacaagcaa gtacctgctg aaatattgga aattgctgct
    20101 aaaatcgaag aggcagatgg cgttattatc ggtactcctg agtatgatca ctctattcca
    20161 gctgttttga tgagcgctct tgcttggttg tcttatggta tttacccact tttgaacaaa
    20221 ccaatcatga ttacaggtgc ttcttacggt acgcttggtt catctcgtgc ccaattgcaa
    20281 cttcgtcaaa tcttgaatgc tcctgaaatc aaggcaaatg ttcttccaga tgaattcttg
    20341 ctctcacact ctcttcaagc atttaaccca agtggcgact tggttgacct tgatgttatc
    20401 aagaaattgg atgccatctt tgatgacttc cgtatcttcg tgaagattac tgagaaattg
    20461 cgcaatgcgc aagaattgct tcgcaaagat gctgaagact ttgactggga aaatttgtaa
    20521 gataggagac caaaaagaat gaaatttgtt ggacttgttg gatcaaacta cgatcaatca
    20581 tataaccgta agctcttgga atttatccgt cgctacttca aactcaaatt tgaattagaa
    20641 gttcttgaaa ttgacgaagt tccaatgttt aaccaagatg aaaaatggga tgaaagtttc
    20701 caattacgtt atttatataa caaaattact cgtgcagatg gtgttatcat tgctactcct
    20761 gagcataacc acactatctc agcttcactc aaatctgtac ttgaatggct ttcatacgaa
    20821 gttcatccat ttgaaaacaa gcctgttatg attgtgggtg catcatacta tgaccaagga
    20881 acatcacgtg ctcaagttca ccttcgtaaa atccttgatg ctccaggtgt taatgcctac
    20941 acgcttccag gtaacgaatt ccttcttggt aaagctaagg aagcttttga taacaacgga
    21001 aatatcacca acgaaggcac tgttaaattc cttgaaactt gcttagataa ctttgttaaa
    21061 tacgtaggag tcgtttcgaa attgaaaaaa ccaaaaccaa tcgaatcaga agacttggat
    21121 tgtggaaaac caatcgctac aaccatcaca gaagttgacc ctgacgatcc agaatgggta
    21181 gaaaaagttg cagcattcac tggtgctgtt tctggtgata cctatgtcaa attggaccac
    21241 ggtatcctaa cagttaacca aattgatatg ttcttgaaag ctatgccatt tgaattgaca
    21301 tacgctgacg acaacaacca attcctctac tacaacaacg ctcaccaaga tccagacact
    21361 atgttcgcta aacgtgtacc acctcaatca ggtagccgta tgtcgactgt tcatggttct
    21421 cttccaccag cacgtatgaa gaacgtagag tgggttatcg gaacacttcg caacggaaac
    21481 caagaatacg tccgtacgat cgttccaggt tctcctgcag gtgttatcaa cactcacaac
    21541 taccaagcaa tgtactatcc tgatggatca tacgctggta tcaatgaaat cgtctttaac
    21601 ttccaaccat ggcttgactg gtacctaaaa gagactggtc aacgtttggt gggtggtagc
    21661 ggaccatttg ctcctgctgc cggaggtcat ggagacgccg atgctacttc tggcgcttct
    21721 gattcagggg atgctggagg ccacggtggt gacgcagacg ctacagccgg tgcaagttac
    21781 taatgatagt ttttaggaac cattatggtt ccttttttag cgaaaaaaga ctagcaaaca
    21841 caaactgcta gtctctttgt tatcgtttca tctatctttg ttaaaagaaa gaatacatat
    21901 actcaatgaa aatcaaagag caaactagga agctagccgc aggctgtact taagtacggc
    21961 aaggtgaagc tgacgtggtt tgaatttgat tttcgaagag tattattatg aatttggatc
    22021 atcaagacta cggccatgta atcctttttc acgttgtact tggcgtagtt tttctggtgt
    22081 aacatcgttt ccttcttcgt ccacaatttt gattccttca atgtggtgac gaacagcgcg
    22141 gcgataacct tcgatgtact cctcacgtag tttggcttgt tccacttttt cttctggtgt
    22201 taagccttct gtttttttct ttttagcaag ctcattgata cgagcaattt ttttcggatc
    22261 catttcttct cctctgtaat aagataaagt ccgtttatca aattttattt agtgttaatt
    22321 tggttaaaac aagcaaactt agctgctttc ttggttagtt gtgacaagat tgccaaacga
    22381 ttttgacgga cagcctgatc ttcagccatt accatagtat tttcaaagaa agcatcaatg
    22441 actgggctaa gcgcaaagag ttgtttcaat tgctgacttg caggtcctga taaaacgagt
    22501 gtttctactg cttctgccaa agctttttct tggtcattct caaatagtgc tgaatcaacc
    22561 gtagcaaccc cttctgcctt ctcagccagg ttaaaggcac gagaaagtga ttcaacagat
    22621 ggtttaaaat cttcttcctt gcttacttct acgagagcac ttgctgcttc caacatatct
    22681 gccacaacaa agtttgaacc tgcaagaact gcttctttga tatcttttgg agtagagccc
    22741 atcatcttat caacacgagc cttgataaag tccataacct ctgctttatt ttcataagtc
    22801 aaactgtcaa atttcaatgc ataaaggcta tcaatcagct catccatagc aatgtgccaa
    22861 ccaaaggcat ccaagatacg aaccacacct tgagttgcac gacgaagggc ataagggtca
    22921 ttagaacctg atggaatcaa tcctactgag aagaaactca aaatcgtatc caatttgtct
    22981 gcaatggcta gaactgcgcc gaccttgctc tctggaagtt ctccttcagc tgatgtaggc
    23041 atgtagtgtt cacgaatagc agctgccacc gctggagttt caccagcaag aagggtgtat
    23101 ttttcaccca taattccttg gagttcgtca aattcaccaa ccatacctgt caacaagtca
    23161 aacttgtaaa tggctgctgc acgagcaagg tcaactgttt catccactga caaactagct
    23221 ttttctgcca aaagtacagt gatttgaccc gtacgaatca tgtgttcacg aagggaacca
    23281 atcttctcat ggaaggtgac attgtttaat ttttcaacaa gatctgaaat caccaatttt
    23341 tggtcttcac gccagaagaa ttctccgtct tccaagcggg ctaccaagac tttttcattt
    23401 cctttgatga catttttcaa acgctctgcg tttccgttac gaacagaaat gaagtttggc
    23461 aagagttttc catcttgatc acgaacaaca aagtaacgct ggtgttcctt catagaagtc
    23521 accaagactt cttctggaac ttcaaggtat ttagcatcaa aacttcccat gaaggcagtt
    23581 gggtattcaa ccaaattcaa gacttcattc agcaaatccg catcaatttc gatacgtaca
    23641 ccatgttttg cctcaatttc cttgatttgg tcaacaatca tttgctcacg ttcacatgga
    23701 tctgcgatta caaactgctt acgaaggtct tcttcatagc tcaatgctga ctgaattttg
    23761 gtttctttcc ccaagaaacg atggccacgg ctcacacgac ttcccttgat atcaaggaaa
    23821 tccaagtcaa actcttgctc atccaagaga acagttaaag tgtgaacagg gcggatgtat
    23881 tcaaagctat ttccagccca gtgcatgctg acagggaaag tcaatgactt caagacatct
    23941 acaacaccag gaacaatggc ttcaactgat tggccaactt cttccttagt gacatagaca
    24001 tattcttcac ccttaatttc acggaattcg atatcttcaa cagtcaaacc tttcccacgg
    24061 acaaatcctt gagctgcttt ggtgaagttt ccatcactat ccaaggcaat tttctttgct
    24121 ggtcccttga aatcttctgt caaatcagac tgtttgtctg ataaaccagt cacacgaaca
    24181 gccaaacgac gtggtgttga gaaggtttga atggcttcaa aagacaggcg gttttccttg
    24241 aggaaggctg ccattttttc gcctagttgt ttttcacttg gtgtgacaac ataggctggt
    24301 aattcttcaa gaccgagttc tactaataag ttttttgtca tgttttttct ccgaaaagta
    24361 tctttttatt ttccagtttg ccttatgtga ttggcacgca agcaaccaac ttcgttgttt
    24421 cattgctaaa attggagcca aaagtctcca attttgccta gtatccttag tttcactaag
    24481 gataccttca tcactgcggc aactggctca gggctcactc tgttcgccca ttttcgtaat
    24541 ttgtcactct ctttattctg cgtcttctgc taggagttta gctcttgttt cttcatccaa
    24601 aagtgggtag cctaggcgtt tgcgttctgc gacaaaggtt ttggctacaa cacgggccaa
    24661 gttacggata cgagcgatat agcctgcacg ctctgttaca gatacggcac cacgcgcgtc
    24721 aagcagatta aaggtatgtg aacatttgag aacatagtca taggcagggt gtaccaagcc
    24781 ttcttctaat gcacgaccag cttctttttc aaacttatca aagttttcaa gcaacatttc
    24841 ttggtccgaa atttcaaatg aatattttga gtgctcatac tcaggctgga taaagatttc
    24901 tccgtatttt acaccatcag cccactcgat atcatagaca gagtctactt cttgaatgta
    24961 agaagccaag cgctccaaac cataggtaac ttccgcagtc acagggccag ttgccaatcc
    25021 accgacttgt tggaaataag tgaactgagt gatttccatt ccgtcaagcc aaacttccca
    25081 accaagacca gctgaaccag ttgatgggtt ttcccagttg tcctcaacaa aacgaatatc
    25141 gtgctccaaa ggattgattc ccaatttttc caaagactca aggtaaagtt cttggatatt
    25201 tgatggagaa ggcttcatga ccacctggaa ttggtggtgt tggtagagac ggttagggtt
    25261 ttccccataa cgaccgtcag caggacgacg tgatggctct acataagctg cattccatgg
    25321 ctcaggtccg atagcacgaa ggaaagtgta aggactcatt gttcccgcac ctttttcatt
    25381 atcataagcc tgcataagca tacaaccttg gtcattccaa aattgttgca aagtaaggat
    25441 gatttcctgg aaagtcaatt tcttagacat tgtttactcc tttttatata aattacttgc
    25501 gacatgagtc tgcctcgtcg atcatacgag gcaagacgag ttagtactgc gtctagctca
    25561 ggcactctag tgctgagcgt ggggcagtcc gactgtaagg aggtttctgc cactgacgca
    25621 gtgaaatgtc aatttcttag acattgttta ctcctttagt ttttatattt ctttttcaat
    25681 taatttctgc tgaacaaacc aatctaacaa gggaaaagtt ttttctgtat ttgcccataa
    25741 atgataagaa tctaagaccg cttgtacact acctaaattt tctaccaact tatctactgt
    25801 caaaaagctg cgccagtatt cataaaaaat acttgcatag ttcttatcat aagtcgaaga
    25861 accaaaatca ttcaatgaat gccaaccata cttcttctga aaaagttcta cgagagattg
    25921 attacaaatt ttttccgctt gaaattcctc ttctgtcaag aaatacttgc gactaatata
    25981 ttcaaccatc ccctcttcga accagatata agagtcataa ccatcaaaat catctaaaaa
    26041 atgctccgac caatgagcta actcatgtcc tacaatctgt aagaggaaat tttcagataa
    26101 agaatgatag tgactttcta ttgcttgagt ttgctgagaa cactcgtagt ccatcaattg
    26161 atacaaatac aactctttcc aaacagctaa atcaggcgcc ataaccattc gtctattatt
    26221 tgtataggct ggaacagcgc tttccctaat aatctgtgta gcagcattaa aatctgacca
    26281 aataacggct tgcggtaaat cacagactgc aagttcattc tttaaaaaag attgataatc
    26341 tttcaacttt tcagtatttc ttactacaaa atcacgaaat gtagctagtt gactatcgtc
    26401 ttttacaaga taaaggttgt ccacggctct cctttctttc acacaataga ctgaataagc
    26461 aaaagaaccg cgtccgacaa tcctaggttg atttaaccta ggggcgtcag aaacgcggtt
    26521 ccaccctaat ttattacttc attgtttttg aaaatactac agaaagcgcc agctcattat
    26581 tttgccctac ttggctccca ctatcccaag ctcgcttgaa gaacgccata agactttctt
    26641 tcctgctgac tattatatca aaaattagaa taatgtacaa ttctttttat gattttctag
    26701 aaatccacat catcaactcg tggagcaccc gattgaacag caatcgtttt taagttttct
    26761 ctctcctcat gactcagttc aattccaaag caatcaagat tagcttgaat acgagaggtt
    26821 gtgacagatt ttggaagtgg taaaaatcct tctgccaagc tccaggccaa ggctatctga
    26881 gcaatcgatt ttccgtgatt tgctgcgatt tcttgcactt gcttgctatc aaatagttct
    26941 ccctgaccaa aaggccccca agcttccaat aaaattccct tttcacgaca gtaagctacg
    27001 acttgatctt gatacacacc tggcgccaag cgaacttgat tgaccgcagg aacgatagtt
    27061 gcagtttcaa gcaaggcatc caaatgatgg ggaagaaaat tgctaacgcc gatagcacgg
    27121 attttccctt cttgatagag gtcttccatc gctctccaaa cttccgcatt gcgagttttc
    27181 catgcgtcat tttctctgag cggttttggg ttcggccaat gaatcaaata caaatccaaa
    27241 taatccaagc ccagtttttc tatagatttt tccaaagctt gacgagtttg ctcataggtt
    27301 tgctgactat tccaaagctt ggtagttacg aacatttctt cacgtggaac tccgctatct
    27361 ttgattgctt gaccaacact ttcttcattc tgataaatcg ccgccgtatc aatatgacga
    27421 taaccagcct tcaaggcttc taacactgca tgataggctt cttctccatc cttagcctta
    27481 aaagttccaa atcccaatac tggaatttct actccattat ttaattgata ggtattcata
    27541 tgcttctcct tctattttat tcatcaatta tagcataaga cattttttaa gtagtactat
    27601 cttctgtaaa aaataagtct gtcaaatatc taacagacct attttttctt gcttattcat
    27661 cacgactata gtacagctca tgagctttta ggcgagtatt gagcatctct ggtatggtca
    27721 caaaggtata accttgattt ttcaaatgct caatgaccct tggcaaggca ttgactgtcg
    27781 gactgtgaat atcatgcatc aaaacgatag agccgttagc tacttgatgc tgaatttctg
    27841 tcaaaataga tgcttcattt ttactcttcc agtccagact atccacatcc cacatgataa
    27901 agctcaaatc caagctattg cgaatatcat ctgtaatagc accataaggt ggacgcatga
    27961 gtttagaact agaacccagc actttagtta gcacatcctc agtatcagta atctgctttt
    28021 tagcttcatc aagagagagt tgcgagagaa tcggatggct ccaactatgg tttccaacaa
    28081 catgaccttc agattttatc ctcttcacca agtcctcatt cccagaaaca tttttcccaa
    28141 gcacaaagaa agtcgcttta atatcatatt tagctagggt ctctaatacc tgcggggtcg
    28201 ttgctggatt tggaccatca tcaaaggtta gagcgacaac tttttgatat ttcttatcaa
    28261 agtaagattg gtacaaggcc gcatcttttt cgagtaagta cgaagattgg ataacatcaa
    28321 agaaagcaga tactggcaag gctatctctt ctaaattttc aagcacagga cttggataaa
    28381 ggataatctg actatccttg taatcaaaat tccatgcaga caagtcttgg tcagagaagt
    28441 tttttacaat ctgctcactc tggtcttgct ctattttttt atcctctatg aagggggtca
    28501 actcttttat cagctgttcc ttagccttac tagcatctga aaacagttgg tcaagtgtaa
    28561 aaggttgccc atcttctgtt aagtgaacct ttcctagact ggtcttttca gtctcttcaa
    28621 ccttaaaagc aactaaatca tagatttgtt tggtcacatt acgattaacg actcccttta
    28681 aacctgactc ttcttgctct gtatagtaga aaacaagatt gtccttactt tctagcttgt
    28741 ccttaacatc ttgatttatc aactccctaa cagaggaaat cactttctcc ccttggagag
    28801 gataataggc aatcacttcg gcttgcccct gacgaaaatg ctccttctga tttccctcac
    28861 tcaattgatc atctttctct tttttgagcg attcaatctt ttgctcaaaa cttttttgct
    28921 ggtagatctt aaaagcaata aatccgccta ataggcaaat agaaattgct aaaataccaa
    28981 tcaaagccaa taatacatgt ctcgttttcc cgtgtctgcc acgtcctagt ctacttttat
    29041 tcatatttat atcataacaa atttgagcaa taatttcaat gatacaattt ggcaatccac
    29101 aattcctcta gcaagccaaa gcatcttaca tcaactccaa catccgtatc tgtagagatt
    29161 atgtagtttt ctatctatgg aatttctagt agattgaagc tagaatagta gacctatact
    29221 tctaaaacat tgttagaaat cgatttgatt gtcctgatcg atttgtcctg ttcttatttc
    29281 attttactat aaaactatga gaagctaggt catctgagaa gtaattacta tcgacaattg
    29341 cgatagcgta tactggtgcg atgtgtttgt agctctggtg ggtatcacct tcacgttggc
    29401 gaattttttc taggttttga ttaacctgac tgcacagata agcccatagg cgattgatga
    29461 aaaaattctg atgatgaacc tgaatttcga tgataacttg gattccatta tctaactccg
    29521 ccaagacgtc tatactagta tagaaatcct gcgctgagta gggcatggaa ggcaagacgt
    29581 gaatgttact tccctccaaa atcgtcacat tttttgctgg caagtccagc atatcgcgga
    29641 taaattgaca agtgatttct ggattgctga atattttttt agcaaccaaa tcattagttg
    29701 ggctgatgcc cggatgtctg agaatcctcc ttctattata gcacattttt aaatctaggt
    29761 ttgctagatt ctatgcttct atctatttat tcggaaaaga aagctaatag cttatttgaa
    29821 attttctcct ctctgttgga tagtcccgtc tatctatgtt ataatgaaag aaggatttaa
    29881 aatcggaaaa ggagtattta tgctaaaatt aggtgtcatc ggaactggcg ctatcagcca
    29941 tcatttcata gaagcagccc ataccagtgg agaataccaa ctggtcgcaa tctattctag
    30001 aaaactagaa actgcagcaa cctttgcttc tcgctatcag aatatccaac tattcgatca
    30061 attagaagtc ttcttcaaat cttcttttga tttagtctat atcgccagtc caaactcctt
    30121 acattttgct caggcaaaag ctgccttgtc tgctggtaaa catgttattc tcgaaaagcc
    30181 agctgtcact caaccacaag aatggtttga tttgattcaa acagctgaaa aaaataactg
    30241 ttttatcttt gaagcagctc gtaattacca cgagaaagcc tttactacta tcaaaaactt
    30301 tttagcagat aagcaagtgt taggagcaga tttcaactat gccaaatact cttccaagat
    30361 gcctgacttg ttgactggac aaacaccaaa tgtcttttca aaccgttttg ctggtggagc
    30421 cctcatggat ttggggattt atcccctcta tgctgctgtt cgtctttttg gaaaagccaa
    30481 tgacgcgact taccatgctc aacagcttga caatagcatt gacctaaatg gagatggtat
    30541 cctcttctac ccagactatc aagttcacat caaggctgga aaaaacatca cttccaatct
    30601 tccttgcgag atttatacaa cagatggaac cttgactctc aacacgattg agcatattcg
    30661 ctcagctatt tttaccgacc accaaggaaa tcaagtccag cttcctatcc aacaggctcc
    30721 tcatacgatg actgaggaag tcgctgcatt tgcacacatg atccagcaac cagatctgaa
    30781 tctctaccaa acttggctgt atgatgcagg ctctgttcat gagctactat ataccatgcg
    30841 ccagactgct ggcattagat ttgaggcaga aaaatgaaaa ccaaactacc gactgaatgg
    30901 caagaactga gtgaccaact cggtttccaa gaattcaccc ccattcaaac tcaactattt
    30961 gagccccttc ttgctggaga aaacctccta ggagtgagcc aaaccggaac tggtaagacc
    31021 ctagcgtatc tcctaccaag tcttctcaga ctacaaaaga aaaaagccca acaactcttg
    31081 attctagcac caaacacgga acttgctgga cagatttttg atgtatgtaa aacgtgggca
    31141 gaagctatcg gattgactgc tcaactcttc ctttcaggtt caagtcagaa acgacaaatt
    31201 gaacgcctta aaaaggggcc agaaattctg attggaactc ctggccgtat ctttgaactc
    31261 attaaactta aaaaaatcaa gatgatgaat gtggaaacca tcatcctgga tgaatttgac
    31321 caattactag atgattctca gattcacttt gtagaaaaaa tcactcacta cgcacctcgt
    31381 gaccaccaac tggtctacat gagtgccacg accaagtttg atcaagaaaa gattgtgccg
    31441 aatacacgca ctattgatct ctctgatcaa aaattggaca atatccagca tttctacatg
    31501 caggtagacc aacgtcatcg agtggatata ctacgaaaac tggctcatgt agaggatttc
    31561 cgtggtctag tcttcttcaa tagcctgtca gaccttggga atgccgagga aaaactacag
    31621 tatcgtgata tattggctgt ttccctcgct agtgatgtta atgttaaatt tagaaaagtc
    31681 atcttagaaa agtttaaaga taaccaacta accctgcttc ttgcaactga ccttttggct
    31741 cgtggaattg atatcgatag cctagaatgt gtcgtaaact ttgatattcc tagagatagc
    31801 gaaacctaca cacaccgtgc tggccgtact ggccgtatgg gcaaggaagg atatgttatt
    31861 actctcgtca ctcatccaga agaaatcaaa aaacttaaaa agtttgcaag tatccgagaa
    31921 attgtcctaa aaaaccaaga actctatatc aaataaagcc ccattgctct atacacatcc
    31981 agtgtatgga gcttttttat aagcaattca attttatact catctgcttt caaaaagcat
    32041 tctattccat ctccgattaa cgatggactt tatcacctcc ttctccagtc cttgtatgac
    32101 atcttggagt tgattcatga catcttccaa agttcgaaag gctttattct taaatccacg
    32161 tttacgaatc tctttccaca cttgttcaat gggttcatct ctggtgtgta tggaggaata
    32221 aaggtaaaat caatattagt cggaatcttt aaggtacttg atttatgcca tatagcattg
    32281 tccataacga gtaaaaaata atcatctgga taagcttgtg aaagctcttc taaaaagacg
    32341 ttcatccact cagtattaca tccaccagct attaagaaaa atgattcgcc tgtatggggc
    32401 atcaacagct ccataacaat agcgaaattc tcgtatatag tgactatgga catgtggacc
    32461 tactcctatt ggagaccaac aagatcccag tttactgatt ctaccgaaac cagcctcatc
    32521 ttggtacatc aagcgaacct tatgaaaagg tctactggtt ttaaagcgct ttcttgtctt
    32581 cttgaattga gattttattt ttagacgcga caatggtttg agcgtctgct ttcttaggat
    32641 gttctggacg tggcataata tttcgccaac catggtgctt caacagttga tagaaggcat
    32701 cacgtgtgta ggaacgatat aagcctgaaa taaggtattg taacaaattc tcctgcctct
    32761 gtagccttca aatggcgggc aagaaaggct ttctcttcct caactgtcat atatgcatgg
    32821 ttacgaccac cacgtgtttc ttgaaggaga gagtcgagtc cgaactcctc atattttttt
    32881 acatttcgcc aaatcgttgt ttgattacag tctaaaagat ctataatctc tttataagat
    32941 ttgcccatca gacgaaatag aacgatttga aggcgtttat gatatttagc tgtacgaaag
    33001 tcttttaaaa gtgttttgat ggtttggatt tcttcttcag ttgatttcat attactattg
    33061 tataatgctt tttgatttga gtctagtatt atactcttcg aaaatctctt caaaccgcgt
    33121 cagcttcgcc ttgtcgtata gatgttactg acttcgtcag ttctatctac aacctaaaaa
    33181 cagtgttttg agcagtctgc agctaacttt ctataacaaa atgaaataag aacaagacaa
    33241 atcaatcaag acaatcaaat cgatttctaa caatgtttta gaagtagatg tgtactattc
    33301 tagtttcaat atattatagt ttgctctttg attttcattg agtattagtt caatatctaa
    33361 aaaaagaggc tctataatat ttgtagtggg taaatcccct atagatatta tggagcctat
    33421 ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa cc
//
LOCUS       DAVKLW010000021        33141 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKLW010000021 DAVKLW010000000
VERSION     DAVKLW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.021"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            59..856
                     /locus_tag="VW324_000937"
     CDS             59..856
                     /locus_tag="VW324_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9726599.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1004..2263
                     /locus_tag="VW324_000938"
                     /pseudo
     CDS             1004..2263
                     /locus_tag="VW324_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2359..3633
                     /locus_tag="VW324_000939"
     CDS             2359..3633
                     /locus_tag="VW324_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9726600.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEHYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3643..4521
                     /gene="truB"
                     /locus_tag="VW324_000940"
     CDS             3643..4521
                     /gene="truB"
                     /locus_tag="VW324_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9726601.1"
                     /translation="MNGIINLKKEAGITSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4782..5084
                     /locus_tag="VW324_000941"
     CDS             4782..5084
                     /locus_tag="VW324_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9726602.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5192..5830
                     /gene="udk"
                     /locus_tag="VW324_000942"
     CDS             5192..5830
                     /gene="udk"
                     /locus_tag="VW324_000942"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9726603.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5958..7298
                     /locus_tag="VW324_000943"
     CDS             5958..7298
                     /locus_tag="VW324_000943"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9726604.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7276..7488
                     /locus_tag="VW324_000944"
     CDS             7276..7488
                     /locus_tag="VW324_000944"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9726605.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7485..8360
                     /locus_tag="VW324_000945"
     CDS             7485..8360
                     /locus_tag="VW324_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9726606.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8353..9168
                     /locus_tag="VW324_000946"
     CDS             8353..9168
                     /locus_tag="VW324_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9726607.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            9161..9592
                     /locus_tag="VW324_000947"
     CDS             9161..9592
                     /locus_tag="VW324_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9726608.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9599..11266
                     /gene="recN"
                     /locus_tag="VW324_000948"
     CDS             9599..11266
                     /gene="recN"
                     /locus_tag="VW324_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9726609.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11268..11996
                     /locus_tag="VW324_000949"
     CDS             11268..11996
                     /locus_tag="VW324_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9726610.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12075..13898
                     /gene="lepA"
                     /locus_tag="VW324_000950"
     CDS             12075..13898
                     /gene="lepA"
                     /locus_tag="VW324_000950"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9726611.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14192..14272)
                     /locus_tag="VW324_000951"
                     /pseudo
     CDS             complement(14192..14272)
                     /locus_tag="VW324_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14446..14922
                     /locus_tag="VW324_000952"
                     /pseudo
     CDS             14446..14922
                     /locus_tag="VW324_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            14960..15262
                     /locus_tag="VW324_000953"
     CDS             14960..15262
                     /locus_tag="VW324_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9726612.1"
                     /translation="MIKILAACGAGVNSSYQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGSILFRIPAMSAPVFDNIILPAKQNMV"
     gene            15270..16073
                     /locus_tag="VW324_000954"
                     /pseudo
     CDS             15270..16073
                     /locus_tag="VW324_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            16122..16586
                     /locus_tag="VW324_000955"
     CDS             16122..16586
                     /locus_tag="VW324_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9726613.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            16743..17168
                     /gene="lacA"
                     /locus_tag="VW324_000956"
     CDS             16743..17168
                     /gene="lacA"
                     /locus_tag="VW324_000956"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9726614.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            17199..17714
                     /gene="lacB"
                     /locus_tag="VW324_000957"
     CDS             17199..17714
                     /gene="lacB"
                     /locus_tag="VW324_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9726615.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            17725..18654
                     /locus_tag="VW324_000958"
     CDS             17725..18654
                     /locus_tag="VW324_000958"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9726616.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLTKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            18656..19636
                     /gene="lacD"
                     /locus_tag="VW324_000959"
     CDS             18656..19636
                     /gene="lacD"
                     /locus_tag="VW324_000959"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9726617.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            19870..20706
                     /locus_tag="VW324_000960"
     CDS             19870..20706
                     /locus_tag="VW324_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9726618.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            20742..21059
                     /locus_tag="VW324_000961"
     CDS             20742..21059
                     /locus_tag="VW324_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9726619.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            21059..22750
                     /locus_tag="VW324_000962"
     CDS             21059..22750
                     /locus_tag="VW324_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9726620.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAKYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            22965..24371
                     /gene="lacG"
                     /locus_tag="VW324_000963"
     CDS             22965..24371
                     /gene="lacG"
                     /locus_tag="VW324_000963"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9726621.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSFNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGKKGSSKYQIKGVGRR
                     VAPDYVPRTNWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            24414..24671
                     /locus_tag="VW324_000964"
     CDS             24414..24671
                     /locus_tag="VW324_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9726622.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDELEEFKNLFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(24950..25711)
                     /locus_tag="VW324_000965"
     CDS             complement(24950..25711)
                     /locus_tag="VW324_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9726623.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(25999..26961)
                     /gene="nrdF"
                     /locus_tag="VW324_000966"
     CDS             complement(25999..26961)
                     /gene="nrdF"
                     /locus_tag="VW324_000966"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9726624.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(27149..29308)
                     /gene="nrdE"
                     /locus_tag="VW324_000967"
     CDS             complement(27149..29308)
                     /gene="nrdE"
                     /locus_tag="VW324_000967"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9726625.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(29389..29607)
                     /gene="nrdH"
                     /locus_tag="VW324_000968"
     CDS             complement(29389..29607)
                     /gene="nrdH"
                     /locus_tag="VW324_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9726626.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            29958..30221
                     /locus_tag="VW324_000969"
     CDS             29958..30221
                     /locus_tag="VW324_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9726627.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            30227..31960
                     /gene="ptsP"
                     /locus_tag="VW324_000970"
     CDS             30227..31960
                     /gene="ptsP"
                     /locus_tag="VW324_000970"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9726628.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            32215..>33141
                     /gene="phtA"
                     /locus_tag="VW324_000971"
     CDS             32215..>33141
                     /gene="phtA"
                     /locus_tag="VW324_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9726629.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGL"
BASE COUNT        10177 a         6013 c         7256 g         9695 t
ORIGIN      
        1 ttcctaaaac ctaaagtttg tgatataatt atcaagaaaa aagatttagg agtttattat
       61 ggtttcttca gaatttatct caaagattga atttgcttgc aataagaaag aaagtcttta
      121 tagtcaaagc aaatttaagt atgcgattcg ttcgatgttc gcaggtgcat ttttaacctt
      181 cagtactgct gcaggtgcag ttggggctga cttgattaat aaaattgcac caggtagtgg
      241 acgcttcctc tttccattcg tttttgcttg gggcttggcc tacattgttt ttttgaatgc
      301 cgagttggtc acttcaaaca tgatgttctt gactgctggt agtttcttaa aaaaaatctc
      361 ttggagaaaa acagctgaga ttttactata ctgtaccttg ttcaacctta tcggagcctt
      421 gatagcaggg tggggctttg ctcattcggc agcctatgcg aatctgacac acgatagttt
      481 catctcaggt gttgttgaga tgaagttagg ccgctcaaat gaattggtct tgcttgaggc
      541 gattttggca aatattttcg taaatattgc gattctgtca tttattttgg tcaaagatgg
      601 tggtgccaaa ctttggcttg tgttgtcagc tatttacatg tttgtattct taacaaacga
      661 gcacattgcg gcgaactttg cttctttcgc gattgtgaaa ttcagtgttg ctgcggattc
      721 aattgccaac ttcggtgttg gaaatatgct tcgccactgg ggtgtgactt tcatcggaaa
      781 ctttatcgga ggaggcctct tgatgggtct tccatatgcc ttcctcaata aaaacgaaga
      841 tacttatgta gattaagaaa atgagcacga ttgagtcgtg cttttttcat tttcaaaata
      901 aggtaatagc tatttcttat atcaaaatat agaaaactga tatttgtaaa ctataactca
      961 aggtgctaca atatccttaa taaaataata tggaggtcac cttatgactc gtgattttaa
     1021 atttgaaact ctacaactac atgctggtca agttgtggct ccagctacta agtctcgtgc
     1081 agtgccgatt tatcaaacaa cattttttgt ttttgatgac acgtaggaag gtgccgatct
     1141 gtttgccttg aggaaaccag ggaacattta tactcgtatc accaatccta caacagctgc
     1201 ccttgaaggt ggtgttgaag cgctagcaac agcatcaggt atgactgcag tgacttatac
     1261 gattttggcg cttgcccatg ctggtgagca tgtagtggct gcttcgacta tttacggtgg
     1321 aaccttcaat cttttgaaag aaccccttcc tcgttatggt atcacaacaa cctttgtcga
     1381 tgttgataat ttggaggaag tagaagcagc tatcaaagac aataccaagc ttgtcttgat
     1441 tgaaaccttg ggtaacccct tgattaatat tccagacctg gaaaaactgg cagagattgc
     1501 tcataaacat caaatcccac ttgtgtcaga caatactttt gcaacacctt atttgattaa
     1561 cgtcttctct catggcgttg acattgccat tcactctgcg actaagttta tcggtgggca
     1621 tggtacaact attggaggaa taattgtcga tagtggtcgt tttgactgga cggcttcagg
     1681 gaaattccct caatttgttg acgagagtcc aagctgccac aatttgagct atactcatga
     1741 tgtgggtgca gcagccttta ttatagctgt tcgagttcaa ttgcttcgtg atacaggtgc
     1801 agccttgtca ccattcaatg ctttcctctt gctacaaaga cttgaaaccc tttcacttcg
     1861 tgtggaacgc catgtacaaa atgctgagac aattgttgat tttcttgtca accatcctaa
     1921 ggtagaaaag gtaaattatc caaaacttgc agatagtcct tattatgcct tggctgagaa
     1981 atacttgcca aaaggtgtcg gttcaatctt taccttccac gtcaaaggtg acgaggaaga
     2041 agcacgcaag gtcattgata atttagaaat cttttctgac cttgcaaacg cggcagatgt
     2101 taaatcgctt gttgtccatc cagcaacaat cactcacggt caattgtcag aaaaagacct
     2161 agaagcagca ggtgtcacac caaatcaaat ccacttgtct atcggtcttg aaaatgtaga
     2221 agatttgatt gaagacttac gcttggcctt ggaaaaaatt taaagtaaaa gaagataaac
     2281 agtgggtttt gactcactgt ttttgatttt ccctcaggca tgatataatg gttacagaag
     2341 tctagaaaga ggaatgtaat gaacgaaatc aaatgtccca actgtgggga agtctttaca
     2401 gtaaatgaaa gccagtatgc tgaacttttg tcccaagtga gaacggcaga gtttgataag
     2461 gaactacacg ataggatgag gcaggaactg gccttggctg agcaaaaggc tatgaatgag
     2521 caacagacta aactggctca gaaggatcaa gaaattgcgc aattgcagag tcaaatccaa
     2581 aactttgata cagaaaaaga attggccaag aaagaagttg aacagacaag ccatcaggcc
     2641 ttattggcta aggacaagga agtacaggcc ttggaaaacc agttggcgac cttgcgtttg
     2701 gagcatgaaa atcaattgca aaagaccctt tctgacctag aaaaagaacg caatcaggtc
     2761 aaaaatcagc tcctactgca agaaaaggaa aatgagttgt ctttggcttc tgttaagcaa
     2821 aactacgaag cccagctcaa agcagccagt gaacaagtcg agttttataa gaatttcaaa
     2881 gctcaacaat ccacaaaagc catcggggaa agtttggaac actatgcgga aagtgagttt
     2941 aacaaggttc gtagtttcgc ctttccaaat gcttactttg agaaggataa caaggtctct
     3001 tcgcgtgggt ctaaagggga ctttatcttc cgtgagtgtg atgaaaatgg agttgaaatc
     3061 atttctatca tgtttgagat gaaaaacgaa gcggacggaa cagagaagaa gcacaagaat
     3121 gcagattttt acaaggaatt ggacaaggac cgtcgggaga agaactgtga gtatgccgtt
     3181 ttggtgacca tgcttgaggc tgataatgac tactttaaca cagggattgt tgacgtcagt
     3241 cacgagtatg aaaaaatgta tgttgttcgt cctcaattct ttatccaatt gattggtctc
     3301 ttacgtaatg cggcgctaaa ttccctaaaa tacaagcagg agttggcctt ggttcgcgag
     3361 caaaatattg acattacgca ttttgaggaa gatttggatg cctttaagct agcttttgct
     3421 aagaactata attcagcttc gactaacttt ggaaaagcta ttgatgaaat cgacaaggcc
     3481 atcaaacgca tggaagaggt taagaaattc ctgactacat ctgaaaacca actccgtcta
     3541 gctaacaaca aattggaaga tgtctctgtt aaaaaattga cccggaaaaa tccaacaatg
     3601 aaagcgaagt tcgaagcact gaagggggag tagaaagcaa aaatgaacgg tattatcaac
     3661 ttaaaaaaag aagcaggaat aacctcgcat gatgcggttt ttaaactgcg taagattttg
     3721 ggaaccaaga aaattggtca tggtggaacc ttggatccgg atgtggtggg tgttttgccg
     3781 attgcggttg gcaaggcgac acgcatggtc gagtttatgc aggacgaggg taagatctat
     3841 gagggggaaa ttactctggg ttattccacg acgactgagg atgctagtgg ggaagtaatc
     3901 gcagaaaccc ctgttttgtc tcccttggat gaaaagcttg ttgatgaagc gattgctagc
     3961 ttgactgggc ctattaccca gattccccct atgtattcgg cagttaaggt taatggtcgc
     4021 aagctctatg agtatgcgcg tgctggtcag gaagtggagc gtccagaacg tcaggtgacc
     4081 atttatcaat ttgagcgaac aagtccgatt tcttatgatg gccaacttgc tcgattcact
     4141 tttcgtgtaa aatgcagtaa agggacttac atccgtactt tgtcagttga tttgggtgaa
     4201 aagcttggtt atgcggctca tatgtcccat ttgactcgta ctagtgctgc tggcttacaa
     4261 ttagaagatg ctcttgcctt ggaggaaatt gctgaaaaag tagaggctgg gcaattagat
     4321 tttctccatc ctttagagat tgggacaggt gaccttgtca aagttttcct aactccagaa
     4381 gaggctacag aagttcgctt tggtcgtttt attgagctag accaaacgga caaagaactg
     4441 gctgcctttg aagatgataa attgttagcc attctagaaa aacggggcaa tctctataag
     4501 ccaaggaagg tttttagcta gatcgtttag gaataaaaat cgggtgatag ataataattg
     4561 cttgataaaa ccccatacta atagtagaat ggttttggga attataatat tccaattgtt
     4621 gcgagttgta ggtactcaaa taatctatta ggtactcaaa taatctatat agaaatttag
     4681 aggtgtgaaa tgaagcaatt taaaattctt tcagataaat atttagagtc cattacaggt
     4741 tctggtggga acttaggccc aggatttggt gtgataattc catgatgcga aatgagtttc
     4801 gagaaagggt ggagcaactt cttcaacaaa aagaaataaa tgaaaatagt gagttgagtc
     4861 acctgtttcg tcttgctata caaaatttag acagaaatga aaaataccaa acggtcatgg
     4921 ccaatttgag tcaagggttg tcactttacc tcatgacgca tcattaccag gcacctaagt
     4981 ctgtcattga ttttggttta tggattgcca aagctcctag ccaggaaaga gggagactgg
     5041 ctttcttgca gatgcttgct caaaccctac agggctttcg ttaaaactgt cgaacatcag
     5101 agggaagact ctggtgtttt ttgctgatga gactataaat tttgtaaaaa atgtgataga
     5161 atagacgacg gataaaataa cggaggatag catgcaaaat agaccaatca ttattggagt
     5221 gacaggtggt tctggtggtg gtaaaactag tgtttcaaga gccattttat cgcattttcc
     5281 tgatgaaaag atttccatga ttgagcatga ttcatactac aaggatcagt ctcatttgac
     5341 ctttgaagag cgtgtcaaaa ccaactatga ccatcctttt gcctttgata cagacttgat
     5401 gatcgagcag attaaggaat tgttggcagg gcgtccggtg gacatcccga cctacgacta
     5461 tacagagcat acacggagta gcaagaccta tcgtcaggag cctcaagatg tctttatcgt
     5521 tgagggcatt ttggtcttgg aggacaagcg tctgcgcgat ttgatggata tcaagatttt
     5581 tgtggatacg gatgacgatg tgcgcattat tcgtcgtatc aagcgtgata tggaggagcg
     5641 tggccgtagc cttgatagcg ttattaacca gtacttaggt gtggtcaaac caatgtacca
     5701 ccagtttatc gagtcaacta agcgttatgc tgatatcgtc attcctgaag gggttagcaa
     5761 taccgtggct atcgacctgt tgacgaccaa gattgcaaag attttggaag aagctcgaaa
     5821 cagcaaataa tcacatgtgg aggccttgcc tcctttttct atttttccct cataatggta
     5881 cataaatgga gatttttagg catatttttg gtataataat acccatgaaa gagcaagtaa
     5941 atgaatagta ggtggagatg gaaaagtatt tatcggtaac aactttgacc aagtatctga
     6001 aaatgaaatt cgataaagac ccatacttgg aacgggtcta tttaactggt caagtttcca
     6061 actttcgtaa acgacctact caccaatatt tctccctaaa ggatgaccat gcagttattc
     6121 aagcgaccat ctggtctggg atttatcaga aattagggtt tgacctggaa gaaggaatga
     6181 agatcaatgt gattgggcgt gtacaggtct atgaaccaag tggtagctac tccatcatca
     6241 ttgaaaaagc tgagcctgat ggggttgggg cgcttgcgat tcagtttgaa caacttaaga
     6301 aaaaattgac agaagaaggc ctgtttcaag aacgcttcaa gcaagctcta ccccaatttt
     6361 ctaagagaat tggggtagta accagccgta gtggagccgt tattcgagat attatcacga
     6421 ccgtcagcag gcgattccca ggtgttgaca tccttcttta tcctaccaag gttcaaggtg
     6481 aaggggctgc ggaggaaatt gctagaaata ttgcgcgtgc taatcaacgg gacgatttgg
     6541 atttgctcat tattggtcgt ggaggtggtt ctattgagga tctctgggcc tttaacgaag
     6601 aaattgtggt acgagctatt tttgaatctc gtttgccagt tatctctagt gtggggcatg
     6661 agacggatgt gaccttggca gattttgtgg cagatcgacg cgctgcaacg ccaacagcgg
     6721 cggctgaact ggcaacacct gtgaccaagt tggatgtatt aactcatttg caaaatcagg
     6781 aaaaacggat ggcaacggca gtccgaaatg ttctatctaa gaaacaagag gctttgaaaa
     6841 aatgcagtca gtctgttatc tttagacaac ctgagcgctt gtatgacggt tatttgcaac
     6901 gcttggacca actgcaactg cgtttgaaac aaagtttgcg aactcggatt tctgataaca
     6961 aacaattagt tcaagcaaga actcatcaat tagtacaatt atcacctgtt accaaaatcc
     7021 aacgctatca agaccgttta ggacagttgg acaagctctt aggtagccaa atggcgttag
     7081 tttatgacgc caaggttgct gaggccaagc gactttcgga agctttgctc atgttggata
     7141 ctagccgaat cgtggcgcgt ggttatgcta ttgtcaaaaa agaagagtcc attgtagatt
     7201 cggttgagag tttgaagaaa aaagaccaag taacgctttt gatgcgagat ggtcaagtag
     7261 aattagaggt taaagatgtc aaaacaaaag aaatttgagg aaaatctagc agaactggaa
     7321 accattgtcc aaagtttgga aaatggtgaa attgctctgg aagatgcgat tactgccttt
     7381 caaaagggca tggtcttgtc aaaagagctc caagctacgc tggacaaggc tgaaaagacc
     7441 ttggtcaagg tcatgcaaga agacggaaca gaaagtgatt ttgaatgaaa aagcaagaaa
     7501 aattagctct tgtcgagtcg gctttggaag atttttatgg agaccagcag tttgcctcta
     7561 gtttacggga gtctgttctc tattctattc atgctggtgg caagcgtatt cggccttttc
     7621 tcttgttaga agttctggaa gccttgcagg ttaccatcaa acctgctcac gcgcaggtag
     7681 ctactgcctt ggagatgatt catacaggga gcttgattca cgatgacctt cctgctatgg
     7741 atgatgacga ttatcgaaga gggcggttaa ccaatcacaa gaaattcggt gaagctatgg
     7801 ccattttggc tggagatgcc ttattcttag acccatatgc cttgattgcg caggcagatt
     7861 tgccaagtca gattaaggtg gacttgattg ccaacttatc ccttgcttca ggtagtctgg
     7921 gtatggtggc agggcaagtt ttggatatgg agggcgaaca ccagcacttg tctttggaag
     7981 aacttcagat tattcatgcc aataagactg ggaagttact agcctatccc ttccaagcgg
     8041 cagctattat agctgaattg tcacctgaaa tgcaggtgaa gctgaaaact gtgggtgaat
     8101 tgattggact tgcttttcaa gtcagagatg atgtactgga tgtgacagct agttttgagg
     8161 aaatcggcaa gacacctcaa aaggatctgc aggcagaaaa atcaacctat cctgccctgt
     8221 tgggcttgga agagtccatt gccttttgta accaaaccct ggatcaagct aatgaaaaat
     8281 tagaagaaat tgcccagcag cttccctttg aaacagaatc gattgtaagt gtagtagaaa
     8341 gtttgagaat caatggctaa ggaaagagtg gatgtactag cttataaaca ggggttgttt
     8401 gaaacgagag agcaggccaa gcgaggtgta atggctggcc tagtcgtagc agtccttaat
     8461 ggagaacggt ttgacaagcc aggagagaaa attccagatg acaccgaatt aaaactcaag
     8521 ggagagaaac tcaagtatgt cagccgtggt ggtttgaaac tggaaaaggc cttgcaggtc
     8581 tttgatttgt cggtggatgg cgcgactacg attgatatcg gggcctctac tggaggtttt
     8641 accgatgtca tgctacagaa tagtgccaag ttggtctttg cagtcgatgt tggtaccaat
     8701 cagttggctt ggaaattacg ccaagaccca cgagttgtta gcatggagca gttcaatttc
     8761 cgctatgctg aaaagactga tttcgagcag gagccgagct ttgccagtat tgatgtgagt
     8821 ttcatttccc ttagtctgat tttgccagcc ttgcatcgtg tcttggctga tcaaggtcag
     8881 gtggtagcac ttgtcaaacc tcagtttgag gcaggacgtg agcagattgg gaaaaatgga
     8941 attattcgag atgctaagat tcatcagaat gtccttgaat ctgtaacagc tatggcagta
     9001 gaggcaggtt tttcagtcct tggcttggac ttttctccca tccaaggtgg acatggaaat
     9061 attgaatttt tagcgtattt gaaaaaagaa aagtcagcaa gcaatcagat ttttgctgag
     9121 attaaagaag cagtagagag ggcgcatagt caatttaaaa atgaataaaa aagagagact
     9181 tgaaaaaatt agaagattgg tgacagatta tcaaatcggc acgcaagaag aaattgtaga
     9241 acatttgaaa gaagcaggta tcactgccac tcaggcgacg gtatcccgag atatcaaaga
     9301 gttaggtatt gtcaaaattc ctttgagaga caacacctat gtctacgaat taccaaaatc
     9361 aatcgtaaaa agtctgcaac tggctgaaga caatatcgaa tcggctgaat tgatggataa
     9421 gatgatcaat ctccaagtta ttccaggaaa tacggctttt gtaaaagctc agttaatcga
     9481 tacttttgca gacaagattt ttagttgttt gactgatgat agctcgattt tagtcattgc
     9541 cagaagtgga agtctagcag aggaaatctt tgaacaagta aaaaattggt aggtctatat
     9601 gttacttgaa atttcgataa aaaactttgc cattattgag gctatttccc tcaattttga
     9661 aaaggggatg actgtcctga ctggtgaaac gggtgcaggg aagtcgatta tcattgatgc
     9721 catgaatatg atgttgggag ctcgtgcgac gacagatgtt attcgtcatg gtgcaccaaa
     9781 ggcagagatt gaggggcttt tctcagttga gaatagtcgt cttttacagg aaatttttga
     9841 tgagcaaggt ttagaattag gtgatgaaat tatcatccgt cgggaaatct tgcaaaatgg
     9901 tcgtagtatc agccgtgtaa atggtcagat ggttaatctg tctgttttgc gagctattgg
     9961 gcaacatctt gtagatattc atggtcagca tgaccatgag gagttaatgc gtccccaact
    10021 gcatatccag atgttggatg aatttggtga tgccgctttt tgggacttga aagaaaccta
    10081 tcaaacgagt tttgatgcct atcggaaaat gcgcaagcag gttctggaag tcaagaaaaa
    10141 ccaacaggaa cacaaggaac gtatcgaaat gttggaattt caaatggcag agattgaggc
    10201 agcaaacttg caggctggag aagacttggc tctcaatcaa gagcgagata aactcctcaa
    10261 ccacaaaaat attgcggata cactgaccaa tgcctacagt atgttggaca atgaagattt
    10321 ttcaagtctg gccaatgttc gttcagctat gaatgacatg gaaagtgtcg aagagtatga
    10381 tcctgaatac cgtgaaattt caagctctct gtctgagacc tactatgttt tagaagacat
    10441 tagcaaacgt ttggaagcta tcattgagga ccttgatttt gatggcaatc gcctgatgca
    10501 ggttgagaat cgtttggacc tccttcatac tattactcgt aagtatggtg ggactgttga
    10561 tgatgttttg ctttattttg ccaagattac ggaagaatac aatctcttga caggcaataa
    10621 tctttcgtct gaggacatgg aagcagagct taagaagttg gaagtcaatc ttgtcaattt
    10681 ggcaggtcaa cttgcttctg ctcgtcataa tttggctcag caactcgaag ctgagattaa
    10741 acaagaactg caagatcttt atatggaaaa agcccagttt caggttcatt ttagtaaggg
    10801 aaaattcagt cgtgggggaa atgaaatggt tgagttttat atttcaacca accctggaga
    10861 agactttaaa cccttggtta aggttgcttc tggaggggaa ttatctcgtc tcatgttagc
    10921 cattaagtct gccttttcac gtaaagaagg caagactagc attgtctttg atgaggtgga
    10981 tacgggagtt tcaggtcgtg ttgctcaagc tattgctcag aaaattcata aaattggtca
    11041 gcatggtcag gttttggcta tctcccattt gccacaagta attgcgattg cagattatca
    11101 attctttatt gagaagatta gtaatgacca ttcaacggtt tcgactgttc gtctcttgac
    11161 agtcgaagag cgagtggagg aagttgccaa gatgttggca ggtgatgatg tgacagaagc
    11221 agccctgacg caagccagag aattgttgag aaacagggag aaataagatg acagattatt
    11281 atgtaattgg agatgttcac ggaaaagctg ggatgctgga agaccttctt aaaacctggg
    11341 atggtcagac ccagttgctc tttctaggtg atttgattga cagaggtgag gatagtcacc
    11401 gtgttcttga aatggtcaag gacttggtgg acaatcaagg ggctatctgt ttgtctggaa
    11461 accacgagta tatgtttttg acttggctag atgacccaga agaaagttat gaccattatc
    11521 gtcgcaatgg tggagataca accattaact ctatcctagg tcgtcccttg gatgcaccag
    11581 ttgatggagt agaagatgcc aagcgggttg ccgctgaagc gtcagacttg gtcgaattca
    11641 ttcgtcaaat gccatttgta gtagagacag acaagtatat atttgttcac gcaggtattg
    11701 atttgacttt ggacgactgg catgaaacaa cagattataa aaaagtatgg cttagaaaac
    11761 cattccacgg agctgaaaat catactggaa aaaccattgt cttcggtcat acaccggtct
    11821 atggtttgtt acagcaagaa cgcggtacat ctgagctttg gactacagaa gatggcaaga
    11881 ttggaatgga tggaggagct gtttatggtg gtgtccttca cgggattgtc tttacagacc
    11941 aaggaatgac agaacaccac tttatcgaaa atgacggctt tgtcgctgaa gattagtact
    12001 cctagcaggg tatggtcttg tcaaaatatc aaaaacaatt tataataaat agataccctg
    12061 aaaggaagag aatcatgaac ttagaagaat tgaaaaaacg acaggggaag atccgtaact
    12121 tctctattat cgcccatatt gaccacggga aatcaactct agcagaccgc attttggaaa
    12181 aaacagagac ggtttcaagt cgtgaaatgc aggcccagct tttggatagc atggatctag
    12241 agcgggaacg tggaattact attaagttga atgccatcga gttgaattac actgcaaaag
    12301 atggggaaac ttatattttc cacttgattg acacaccagg gcacgttgac tttacctatg
    12361 aagtttcacg ttcgctagct gcctgtgagg gtgctatttt ggtggtcgat gcggctcaag
    12421 gaattgaggc tcaaactctt gccaacgttt atctggcttt ggataatgat ttggaaatca
    12481 tgcctatcat taataaaatt gacctgccgg ctgcagatcc ggagagcgtg cgtacagaga
    12541 ttgaagatgt gattggtttg gatgccagtg aagcagtttt ggcttctgcc aaggctggta
    12601 ttgggattga agaaatcctc gagcaaattg tagaaaaagt accagcacca acgggtgatg
    12661 tgacggcgcc acttaaggcc ttgattttcg actctgttta cgatgcttac cgtggggtta
    12721 tcctccaagt gcgtgtcatg gacggagtgg tcaaacctgg tgataagatt cagctcatga
    12781 gcaatagtaa gacctttgat gtggccgaag tcggtatttt tacaccaaaa gcggttggtc
    12841 gtgatttcct tgcgactggt gatgttggtt acattgcggc gtctatcaag acggttcagg
    12901 atactcgtgt gggtgatacc gttaccttgg caaccaatcc tgcggcagaa ccattacatg
    12961 gttataagca gatgaatcct atggtctttg cgggtctcta cccaatcgaa tcaaacaagt
    13021 acaatgacct gcgtgaagcg cttgaaaaat tgcaactgaa tgatgctagt cttcagtttg
    13081 aaccagaaac atctcaggca cttggatttg gtttccgttg tggatttctt ggacttctcc
    13141 atatggatgt tatccaggaa cgtttagagc gtgagttcaa tattgatctc atcatgacag
    13201 ctccgtctgt tatttacaaa gttaatttga ccgacggtga gtctatggat gtgtctaacc
    13261 catctgagtt tccagaccca actaagattg cgaccattga agagccttat gtcaaggcgc
    13321 aaatcatggt accacaggag ttcgtcggag cagtaatgga gctagctcag cgtaagcgtg
    13381 gggactttgt gactatggac tatattgatg ataaccgtgt caatgttatc tatcaaattc
    13441 ctcttgctga aatcgtcttt gacttctttg ataaacttaa gtcttcgaca cgtggttatg
    13501 caagttttga ctacgaattg tcagaatatc gcccatctaa gctagtgaaa atggatatcc
    13561 ttctcaacgg agacaaggtg gatgccctca gctttatcgt tcataaggac tttgcttatg
    13621 aacgtgggaa actcatcgtt gataagctca agaaaatcat ccctcgccaa caatttgagg
    13681 ttccaatcca agcggctatt ggacacaaga ttgtcgctcg tactgatatc aaggcccttc
    13741 gtaagaacgt acttgctaaa tgttatggtg gtgacgtttc tcgtaagcgt aaactccttg
    13801 aaaaacaaaa agctggtaag aaacgcatga aatccatcgg atcagttgaa gttccgcaag
    13861 aagccttcct cagcgtcttg agtatggatg aagaataagt tatcagaaat gcctttcctt
    13921 gttcacagga aatttatata agaatcaata gtaaagcagg cttagatggt ctgctttttt
    13981 agtttttata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtatgtat
    14041 ggttactgac ttcgtcagtt tcatctacaa tctcaaaacc atgttttgag ctgacttcgt
    14101 cagtttcatc tacaacctca aaaccatgtt ttgagctgac ttcgtcagtt tcatctacaa
    14161 cctcaaaacc atgttttgag ctgacttcgt cagtttcatc tacaacctca aaactgtgtt
    14221 ttgagtaacc tgcggctagc ttcctagttt gctctttgat tttcattgag tattagaaca
    14281 gaaaaatgct tggagtattt gtttgtgtgt ttatttttat ataacaaact ataaacacaa
    14341 caaaaatata aaaaaagaga caaaaaagaa cagaaagtaa ttaacaacga tttcatatag
    14401 tgttatactt atagcataaa gttaatggaa agaagggaaa accttatggt attagattat
    14461 ttctttgaca aaaaccttgt gttttgctta gaagcggata atcaagaata actctttgat
    14521 caggtagcaa gcttgttgga ggaacgagaa attgtaactc caacttatcg tgaagctttg
    14581 atcacgcgtg aaaagtcatt tccaactggt ttagatatgg aatttctagg aaaggacttg
    14641 ccaaatgtag cgattcctca cacagatatt gttcataatc tagctgaaaa agtggtggtt
    14701 gttcgattag aaaaaccagt aacttttcac aatatgatag ctcctgataa ggaagtagaa
    14761 gtatccctac tcttctttat cattaacaat tcaagttcaa gtcaaacaaa tattctggct
    14821 cagttgatgg actttttcac aggaaatgga catcttgaag atttatcaaa aatttccgaa
    14881 ccagaaaaac tttatgctta cattgctgaa gcaatcgctt aatcttgtct attaaaaaat
    14941 aaaatcggag gaaatccaaa tgattaaaat tcttgctgcc tgcggtgcag gtgttaactc
    15001 aagttaccaa attaaaagtg ctctagaaga agaactttca aaccgtggtt atgatgttca
    15061 ctgtgatgca gtcatggtga aagatgtaaa cgaagacctt atgaaaggtt acgatatctt
    15121 tacaccaatc gctgcaacag atcttggttt tgaaccaggt atcccagtta tcgaagctgg
    15181 gtcaatctta ttccgtatac cagcaatgag cgctccagta tttgacaata ttatacttcc
    15241 tgcgaaacaa aatatggtat agtagttcta tgaatgatga agcaagtaaa caactaactg
    15301 atgcacgatt taagcgtctt gttggtgttc agcgtaccac ttttgaagag atgttagctg
    15361 tattaaaaac agcttatcaa cttaaacacg caaaaggtgg acgaaaacct aaattaagcc
    15421 tagaagacct tcttatgccc actcttcaat agtgcgagaa taccgcactt atgaagaaat
    15481 tgcggctgat tttggtattc acgaaagcaa ctttatccgt cggagccaat gggttgaaat
    15541 aactcttgtt caaagtggtt ttacggtttc aagaactcct ctcagttctg aggacacggt
    15601 aatgattgat gcgacggaag taaaaatcaa tcgccctaaa aaaacaatta gcgaatgatt
    15661 ctggtaaaaa gaaatttcac gctatgaagg ctcaagagat tgtcacaagt caagggagaa
    15721 ttgtttcttt ggatatcact gtgaactatt gtcatgatat gaagttgttc aaaatgagtc
    15781 gcagaaatat cggacaagct ggtaaaatct tggctgacag tggttatcaa gggctcatga
    15841 agatatatcc tcaagcacaa actccacgta aatccagcaa actcaagccg ctaatagctg
    15901 aagataaagc ctataaccat gcgctatcta aggagagaag caaagttgag aacatctttg
    15961 ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt aaacgcttcg
    16021 gattacgaat gaatttgatt gctggtatta tcaatcataa actagaattc tagttttgca
    16081 ggaagtctaa taaagaaact agcaaggtca ggccttactt tattggtgga catccaggat
    16141 ttaattgtct tctacttgat gatgaagtct atgaagatta ttatctagag tttgagaaag
    16201 aagagatttg ctctgttcca cgtcctttcc cagaaacagg tatgttggat ttccaagata
    16261 gaagtccatg gctagaggga caaaaagaaa tagatcttag ttatgatctt ttcagcacag
    16321 atgcagtgac tttggatgaa ttgcaatctc gaacaattgc ccttcgttct cttaaacatg
    16381 ataagggatt gaaagtgcat tttgcagaat ttccaaacct catcatctgg tcaactttga
    16441 acaagggacc tttcattacc tttgaaccat ggtctggctt gtcaacattc cttgaagaag
    16501 gagatcattt agaagataag aagaatgttt gtctcttaga agccaatcag gttgaagaat
    16561 tagggtttga gatagaagtt ttataaaaaa gcaaaaaata aataacaaaa acaaacacaa
    16621 actgttgact tcttcaaaca atagtagtat attatgtttg tgaggactaa ttgactgttt
    16681 tttaaagaca gccaatacac tgctggaatc cagcatagaa aaaataaaaa ggagtataca
    16741 atatgtctat tgttatcggt gcagatgctg caggtttgag attgaaagaa gttgtgaaag
    16801 acttcttaga aaaagaaaac ttccaccttg tggatgttac agctgaaggt caagactttg
    16861 ttgatgtgac tctcgctgtt gctgcagaag taaacaaaga agaacaaaac cttggtatcg
    16921 tgattgatgc ttatggagct ggtccattta tggttgcaac taagatcaaa ggaatggttg
    16981 ctgcagaagt atctgacgaa cgttcagctt atatgactcg tggccacaac aactcacgta
    17041 tgatcactat gggggcacaa cttgttggtg atgaattggc taaaaatatc gctaaaggat
    17101 ttgttaacgg taaatacgac ggtggtcgtc accaaattag ggtcgacatg ttgaacaaaa
    17161 tgggctaatt agattatagt aagaaaggta agttaaaaat gagaattgca attggatgtg
    17221 accacatcgt aacagatgaa aaaatggcgg tttcagaatt tttgaaatca aaaggatatg
    17281 aagtcattga ctttggtacc tatgaccata cacgtactca ctacccaatc tttggtaaaa
    17341 aagtagggga agcagtaact agtggtcaag ctgatctcgg ggtatgtatc tgtggtactg
    17401 gtgttggtat caacaacgct gtaaataaag ttccaggtat tcgttctgcc ttggttcgtg
    17461 atatgacaac agccctttat gctaaagaac aattgaacgc caacgttatc ggttttggtg
    17521 gtaagattac tggtgaattg cttatgtgtg atatcatcga agctttcatt catgctgaat
    17581 acaaaccatc tgaagaaaac aaaaaattga ttgcgaaaat tgaacacctt gaaagtcaca
    17641 atgctcaaca aacagacgca aacttcttca ctgaattcct tgaaaaatgg gatcgcggag
    17701 aataccacga ctaagaggta acctatgatt ttaacagtta caatgaaccc atccattgat
    17761 atttcctatc ccttggatga gttgaagatt gatactgtca atcgtgtggt ggatgtaacc
    17821 aaaacggctg gtggtaaggg actcaatgtt acccgagtac tttcagaatt tggcgattct
    17881 gttcttgcta ctggtttagt gggtggcaaa cttggtgagt ttttggttga acatatcgat
    17941 aatcaagtaa agaaagattt cttctcaatt cagggagaaa ctcgtaactg tatcgctatt
    18001 ctccacggag acaaccaaac agaagttctt gaaaaaggtc ctgaagtatt ggaacaggaa
    18061 ggtcaagact ttttggaaca tttcaaaaaa ctcttggagt cagttgaagt agtggccatc
    18121 tcaggtagtc tgccagctgg ccttccagtt gattactatg cgagcttggt agaacttgct
    18181 aatcaagctg gcaagcctgt agtcttggac tgctcaggtg cagcacttca ggctgttctt
    18241 gaatcacccc ataaaccaac agtcatcaaa ccaaataatg aagaattgtc tcagcttctt
    18301 ggaagagaag tttctgagga tttggatgaa ttaaaagaag tacttcaaga acctttgttt
    18361 gcagggattg aatggattat cgtttcactt ggtgccaacg gtacttttgc caaacatggt
    18421 gacactttct acaaggtaga tattcctaga attcaggtgg taaatcctgt tggatctgga
    18481 gactctactg tggcaggaat ttcttcagga cttcttcaca aagaatcgga tgcagaatta
    18541 ctcaccaagg caaatgtcct tggtatgctc aatgctcaag aaaaaatgac tggtcatgtc
    18601 aacatggcca actatcaagt tctatatgat caattaatag taaaagaggt ataaaatggc
    18661 tttaacagaa caaaaacgtg tacgcttaga aaaactttct gatgaaaatg gtatcatctc
    18721 agctcttgca tttgaccaac gtggtgcttt gaaacgcctc atggctcaac accaaacaga
    18781 agaaccaact gtggcccaaa tggaagaact taaagtcttg gtagcagatg aattgactaa
    18841 atacgcttca tctatgcttc ttgaccctga gtatggactt ccagcaacta aagctcttga
    18901 tgaaaaagct ggtcttctcc ttgcttatga aaaaacaggt tatgacacaa caagcacaaa
    18961 acgcttgcca gactgcttgg atgtctggtc tgcaaaacgt attaaagaag aaggtgcaga
    19021 tgcagttaaa ttcttgcttt actatgatgt agatagctca gacgaactca atcaagaaaa
    19081 acaagcctac atcgaacgca tcggttctga gtgtgtggct gaagatatcc cattcttcct
    19141 tgaaattctt gcttacgatg aaaaaattgc ggatgcaggt tctgtagaat acgcgaaagt
    19201 aaaaccacac aaagttatcg gcgctatgaa agtcttttca gacccacgct ttaacattga
    19261 tgtcttgaaa gttgaagttc ctgttaacat taaatatgtt gaaggcttcg ctgaaggtga
    19321 agtagtttat acacgtgaag aagcagcagc cttcttcaaa gcgcaagatg aagcaacgaa
    19381 cttgccatac atctacttga gtgctggtgt atcagctaaa ctcttccaag atactcttgt
    19441 atttgctcat gaatcaggtg caaactttaa cggagttctt tgtggccgtg ctacatgggc
    19501 agggtcagtt gaagcttaca tcaaagatgg tgaagcagca gctcgcgaat ggcttcgcac
    19561 aactggattt gaaaacattg atgagctcaa taaagttctt caaacaacag cgacttcatg
    19621 gaaagaacgt gtgtaagaaa gtcctcctag tttaggaaca tgaatctaaa aaaatttaaa
    19681 aaaagttgta tgtaaaggtt tacaaaataa cttacttgtg ctatacttaa atcacaagtt
    19741 aatacaaggt gagtgttact aagtaatatt aggcatgatc acaggtgaat tagaaatcag
    19801 ctgattttct agttcatttg tggtcatttt ttgtacttat atacctttaa gatataaaag
    19861 gaggttgaca tgtatcgaat tctaaatcca atgaatcaca atgtctcgct tgtcagaaat
    19921 gataagggag aagaggtgat tgtaattggt aaggggattg cattcggaaa gaagaagggg
    19981 gatttgattg ctgaaaatca ggttgagaaa atctttcgga tgaagaccga agagtccaga
    20041 gaaaacttta tggctcttct caaagatgtt ccgcttgatt ttatcacagt gacctatgaa
    20101 atcattgata agctatcaaa gaaatatcat tatccgattc aagagtatct ctatgtaacc
    20161 ttgacagatc atatttactg ttcttatcaa gctctaactc aaggaaggta caaggatagt
    20221 aatctgccag atatttccgc taagtatcct gtcgcttttc aaatcgcaaa tgaagctttt
    20281 gaaatttacc gtcagaagct agcagatcat tttcctgagg acgaaattat tcggattgct
    20341 tatcatttca ttaatgctga aggtgaaaat gaagtggaac ttgtggagtc gattgataag
    20401 aggaaagaaa ttctcaggaa tgttgaagaa gttttaacgg actatgcaat tcaacgaact
    20461 aaaaagaata accatttcta tgatcgcttt atgatccatt tgaattattt cttggattat
    20521 ttagacagat ctagagatga taaccaatca cttctggata tggaagatca tattaaacaa
    20581 tcctatccaa aagccttcga gattggttcc aagatctatg atgtgattac gcaacatacg
    20641 ggtcttgatt tgtataaaag tgaacgagtt tatctagttc tacatatcca acgtttattg
    20701 tcataaaaat ttatttaaaa ctatataagg agaattctat catgaataga gaagaagtaa
    20761 cattgttagg ttttgaaatc gtagcctatg ctggcgatgc tcgttcaaaa ctattggaag
    20821 ccttgaaggc tgctgaagct ggtgattttg aaaaagcgga cgctctggta gaggaagctg
    20881 gtagctgtat tgcagaggct caccacgcgc aaacaagtct attgactaag gaagcttcag
    20941 gtgaggactt ggcttatagt gtaaccatga tgcatggcca agaccactta atgacaacta
    21001 tcttgttaaa agatttgatg catcatttaa ttgaactcta caagagagga gttaaataat
    21061 gaataaacta attgcattta tcgagaaagg aaagcctttc tttgaaaaac tatctcgtaa
    21121 tatctatctt cgtgctattc gtgatggttt cattgcaggt atgcctgtta ttctcttctc
    21181 aagtatcttt atcttgattg cctttgtacc aaactcatgg ggctttaaat ggtctgatga
    21241 agttgtagcc tttctgatga aaccttatag ctattctatg ggtattctgg ctctcttggt
    21301 agctggtaca acagctaagt cattgactga ctcagtaaac cgtagcatgg aaaaaaccaa
    21361 tcaaatcaac tatatgtcaa cattgttggc agcaattgtt ggtttgttga tgttggcagc
    21421 tgatcctatc gaaagtggtc tagctactgg attcttgggg acaaaaggtt tgctttcagc
    21481 cttccttgct gccttcgtta ctgtagccat ctataaggtt tgtgttaaga acaacgtcac
    21541 tattcgtatg cctgacgaag ttccaccaaa tatctcacaa gtctttaaag atgtgattcc
    21601 attcactctc tcagttgttt cactttatgc tcttgattta ctagctcgtc attttgttgg
    21661 tgcaagcgta gctgaatcaa tcggtaaatt ctttgcacca ctcttctcag cagcagacgg
    21721 ataccttggt attaccatta tctttggtgc ctttgccttc ttctggtttg tgggtattca
    21781 tggtccatct attgttgaac ctgctatcgc agcaattacc tatgcaaatg ctgaagtcaa
    21841 cttgaacctt ctccaacaag gtatgcacgc tgacaagatt cttacttctg gtacacaaat
    21901 gtttatcgtt accatgggtg gtactggtgc gacactggtt gttccattca tgttcatgtg
    21961 gttaacaaaa tcaaaacgta accgtgcaat cggacgtgct tcagtagttc ctaccttctt
    22021 cggtgtaaat gagccaatct tgtttggtgc acctcttgtt ttgaatccaa tcttcttcat
    22081 tccatttatc tttgctccaa ttgcaaacgt atggatcttt aaattcttta ttgaaactct
    22141 tggaatgaac tcattcactg ctaatctacc atggacaacc ccagctccac taggtctagt
    22201 tcttggtact aacttccaag ttctatcatt cattcttgct gcccttctaa tcgtggttga
    22261 cgttgtcatt tactatccat tccttaaggt ctatgatgaa caaattcttg aagaagaacg
    22321 ttcaggtaag tctaatgatg aattgaaaga aaaagttgct gcaaacttca acactgcaaa
    22381 agcggatgct attcttgaaa aagcaggtgt agaagcagca caaaatacaa tcactaaaga
    22441 aacaaatgtc ctcgttcttt gtgcaggtgg aggaacaagt ggtctccttg caaatgcttt
    22501 gaataaggca gcagcaaaat acaatgtccc tgtgaaagca gcagcaggtg gctatggtgc
    22561 tcaccgtgaa atgttgccag agtttgatct tgttatcctt gcccctcaag ttgcttcaaa
    22621 ctttgaagat atgaaggcag aaacagataa gctcggtatt aaacttgcta agacagaagg
    22681 cgctcaatac atcaaattaa ctcgtgatgg aaaaggtgcc ctagcattcg tacaagagca
    22741 attcgattaa ggctaggaac tgtgaaacag tctcccatcg ttacggaaat cgctatggcg
    22801 aatttcctaa tcgccttatt aattcgtcgg taaaaagata tcgtttttac ctcctcatgt
    22861 cacaattcag tgacttggca caagaagtga gatggagaag catggctcac tgactcctct
    22921 cctctcactt ttactttatt taaatcaaga aataggtgaa aaaaatgaca aaaacacttc
    22981 caaaagactt tattttcggt ggcgcaacag ctgcttatca agcagaaggt gctacacata
    23041 ctgatggaaa aggaccagtt gcttgggata aatatcttga ggataactac tggtacactg
    23101 ccgaaccagc tagtgatttt tacaatcgat atccagttga cctcaagcta gcagaagagt
    23161 atggtgtcaa tggtattcga atttctattg cttggtcacg tattttcccg actggttacg
    23221 gccaagtaaa tgctaaaggt gttgagtttt atcataattt atttgcagag tgtcacaaac
    23281 gtcatgttga gccctttgta actcttcatc actttgacac tccagaagct ctccactcaa
    23341 atggagactt cttaaaccgt gaaaatatcg aacattttgt agactacgct gccttctgtt
    23401 ttgaagaatt tccagaagta aactattgga caacctttaa tgaaattgga ccaatcggtg
    23461 atggtcaata tttggttggg aaattccctc caggtatcca gtacgacctt gccaaagtct
    23521 ttcaatcaca ccacaatatg atggtgtctc atgcacgcgc ggtaaaattg tacaaagaga
    23581 aaggctataa aggggaaatt ggtgttgttc acgccctgcc tactaaatat cctctagatc
    23641 ctgaaaatcc agcagatgtt cgtgcagctg agttggaaga tatcatccac aataaattca
    23701 tcttagacgc aacttatcta ggtcgctatt cagctgaaac catggaaggt gtcaaccata
    23761 tcttatcatt caatggtggt agtttggatc ttcgtgaaga agattttaca gcattagaag
    23821 ctgcaaaaga cttgaatgat ttcctaggaa tcaactacta tatgagtgac tggatggaag
    23881 cctttgatgg agaaactgaa attatccata atggtaaagg taaaaaagga agctctaagt
    23941 atcaaatcaa aggtgttggt cgtcgtgtag ctcctgacta tgtaccacgc acgaattggg
    24001 attggattat ctaccctcaa ggtttgtatg accaaatcat gcgtgtgaag aaagattatc
    24061 ctaactacaa gaagatttac atcactgaaa atggtctcgg ctataaagat gagttcgttg
    24121 ataacactgt ttacgatgat ggtcgtattg attacgtgaa acaacacttg gagatcttgt
    24181 ctgatgcgat tgcagatgga gctaatgtaa aaggttactt catttggtca ttaatggatg
    24241 tcttctcatg gtcaaacggt tatgagaaac gttatggtct cttctacgta gattttgaaa
    24301 ctcaagaacg ttatcctaag aaatcagctc actggtacaa gaaagtagcg gaaactcaga
    24361 ttatagacta gtagaattag tcattagata tagaatttta gtgagtcaaa aagatgttca
    24421 aagattttat ccaatctatt tatgaaaaag tttatattat aaatttcgaa aaatgctctc
    24481 aaataccgtg tttgacgagt gaagaattga aaagtcttgg aaaatggtat gtctcgactg
    24541 gtaaagaatg gatttgtcat tcagatgatg agctggaaga atttaaaaat ctatttttaa
    24601 attttatcaa tcctgaagaa tgggatacta tctcctttga ttcagatttt atgccgtttc
    24661 aacaatcgta accaatttct caaaaaagtt aaatcttata tttagtactc tgtaaaactc
    24721 ttatctaatc acgttactta tactcaatga aaatcaaaga gcaactttaa actaggaagc
    24781 gagtcgcaga tttctcaatg tatagctttg aggaattggg caaaaagtct ttgatataga
    24841 aaaaagcata gtatcaggtg ttgaaacacc tgatactatg ctttttattg tgggaagatt
    24901 tacttttttt cttctgaaat tgagttgtta cccaggctct ttcagtttat taaggcttga
    24961 tgactttaat gtgtttagat agcttaaaaa ggattgaatc acttagttta gaatctgaaa
    25021 caatagtatc aagatttgat acattataaa aagtataaaa atcaaactta ttgaacttac
    25081 tatgatctgc gagtaaatat tttttattag aattatttaa agcgatgcgt tgagcctctc
    25141 cctcttcctc gctaaaagta gctagagctc cgttttgaat accattacag ctaacgaaag
    25201 ctttagaaaa ttggagatta gagagatttt gtagggtcaa tgtaccaaca aaagcacctg
    25261 taatatcgcg ataatttcca cctattaaaa tcaaatctgt taattttcgt tcgcttaaaa
    25321 tcagaaaaac aggtagactg ttggttacga cgcggatatt gtcaataggc aactcacgcg
    25381 caaaaaactc taatgttgtt cctggtccaa tgaaaatagt ttctctttct tctactagac
    25441 tgcctgcaaa atgggctatt tcttgttttt ctgccgtttg gagggcttgt ttttcaatat
    25501 ttgatcgctc attagtcaaa agggagttgg ttcgaagttt ttcagctcca ccatgcacac
    25561 gaatcagcaa atctttatca gctaattcct gtaaatagcg ccttgcagtc atatctgaaa
    25621 cggctatttc gtccataatc tgtttaactg ttatggttcc tttactattt actatctcta
    25681 aaattttggt taatttttct tgtttgagca tattatcacc tcgtttccta ctactatctt
    25741 accataaaca aactcatcat tcaaatacaa aaacaacaaa atgaaacaaa aacaaaaata
    25801 tcgaagtttg ttttcaaaac tttcgatatt tttgttgggt tattattttg ttgtttctag
    25861 tttacttttt gatggttaag agtggtggag aattatactc aatgaaaatc aaagagcaaa
    25921 ctaggaagct agccgcaggc tgtacttgag tacggcaagg cgaagctgac gtggtttgaa
    25981 tttgattttc gaagagtatt agtccaaacc gtagttgtag tcatcatctt gcatggcttc
    26041 aacttcgcca agaaggtaac catttccgac ttgagagaag aagtcatggt tggaagttcc
    26101 tgttgaaata ccgttcataa cgattgggtt gacatcttca gctgaatctg ggaaaagtgg
    26161 atcttgtccc atgttcatga gagctttatt ggcattgtag cgaaggaagg ttttaacctc
    26221 ttcagtccaa ccaacaccgt cataaagact ctctgtgtag ccttcttcat tttcataaag
    26281 agtatagagt aggtcgtaca tccattcttt gagtttttct tgctcttctt caggcaattc
    26341 attgaaacca agttggaatt tgtaaccaat gtaggttccg tgaacagact cgtcacgaat
    26401 aatcaattta atgatttctg caacgttggc tagtttgttg ttaccgagat agtagagggg
    26461 agtgaagaaa ccagagtaga agaggaaggt ttcgaggaag acgctggcaa ctttcttttc
    26521 aagtgggctg ccgtttaggt agatttcgtt gacaatctca gccttctttt gtaggtaagg
    26581 attggtattg gtccattcga aaatttcttc aatctcagcc ttagtattca aggtagaaaa
    26641 gattgatgag taagatttag cgtggacaga ttccataaat tggatgttat tgaaaacagc
    26701 ttcctcatgt ggtgtacgga tgtctgcgcg aagggcttga accccagttt cagattgcat
    26761 agtgtcaaga agggttaaac caccaaaaac ttttccgacc aagtctttct ctttgttaga
    26821 tagctttctc cagtcatcca agtcgtttga taagggaata cgtgtatcga gccaaaattg
    26881 ctccgtcagt ttttcccaag ttgatttgtc gatgacatct tcgatggcat tccagttaat
    26941 ggctttgtag taagtttcca tttaaaatct ctttctgtgt ttagtattgc gaactcacaa
    27001 ttatttctac tttaccataa ttctatagga gtatcgcaca aaaagtcgga agcccgactt
    27061 ttaaaatgtt acataaatta tgttatgaca tagtagattt gattttatca gtgctgctta
    27121 gggaaaaata atgtttctat gctagaaact aaatcacaca gctttcacat tggttggcgc
    27181 cgacttctcc accgtcatct gtaaaggtac ggacgtagta gatagacttg attcccttgt
    27241 taaaggcata gttacgaagg atggacaagt cacgtgtcgt ttgtttattt tccctcttcc
    27301 attcgtaaag gccttttgga atgtcactgc gcatgaagag ggtgagtgaa agtccttgat
    27361 ccacgtgttc agtcgcagca gcgtaaacat cgatgacttt acgcatatcc atatcgtagg
    27421 cagaagtgta gtaaggaatg gtttctgtag acaagccagc agcagggtag tagattttac
    27481 caattttctt ctcttggcgt tcttcgatac gttgcgtaat cgggtggata gaagcagaaa
    27541 cgtcgttgat atagctgata gaaccatttg gcgctacagc aaggcgattt tggtggtaaa
    27601 gaccatcttc ttgaaccttg tcgcgaagtt cagcccaatc agcaacacca gggataaaga
    27661 catttttgaa gagttcttta acacggtctg atgttggaac aaattcacca gttacatact
    27721 tgtcaaagta acttccgtta gcatagtctg atttttcaaa gttgtggaag gtaataccac
    27781 gttcacgtgc aatattgttt gactctacca aggtccagta gttcataagc ataaagtaga
    27841 tgcttgtaaa ttcaacagac tcaggtgaac catattcaat gagttgttgg gcaaggtagc
    27901 tgtgcagtcc catggcaccg agaccaaagg tgtgggcttg gctatttcca tggtcaatcg
    27961 ttggtacagc tacgatatgt gaactatctg taacgaaagt aagggcacga accatagcac
    28021 ggatagaacg accaaaatca ggtgaagtca tcatgttaac cacgttggtt gaacccaggt
    28081 tacatgaaac atctgttccc atttgaagga attcttgagc atcgttgatc aagcttggtt
    28141 cttgaacttg aagaatctca gaacacaagt tactcatgat aatctttcca tcaacaggat
    28201 ttgcacggtt agccgtatcg atgttgacta cataaggata gccagactct tgttgcaatt
    28261 tagagatttc agtttccaaa tcccgcgcct tgatttttgt cttgcgaata tttggatttg
    28321 cgaccaattc atcgtatttt tcagtaatgt cgatgtaatt gaatggcaca ccgtattctt
    28381 tttctacaga gtaagggctg aagaggtaca tttcttcatt tttacgagcc aattcgtaga
    28441 atttatcagg tactacaaca ccaagtgata gagtcttgac acgtactttt tcatcagcgt
    28501 tttctttctt agttgaaagg aaagcgatga tatctgggtg aaagacgttg aggtagacaa
    28561 caccggcacc ttgacgttgc cccaattggt tggagtaaga gaagctgtct tcaaaaagct
    28621 tcataacagg aacgacacct gaagcagctc cttcatagcc tttgataggt gcaccagctt
    28681 cacgaaggtt gctgagggta attcccacac caccaccaat acgtgaaagt tgaagagctg
    28741 agttgataga acgtccgata gagttcatat catccgtcac ttggattagg aaacaagata
    28801 ccaactcccc acgacgagca cgtccagcat tcaagaagga aggagtagca ggttggtagc
    28861 gttggtggat gatttcattg gcaatatcga ttgcaacagc ttcattccca tcagcgaaat
    28921 aaagggcgtt gaagaagaca cggtcttcca tattttcaag atagtattca ccgtcattag
    28981 tctttaaggc atattgattg taaaatttat aagctgccat gaatgacttg aattggaagt
    29041 tttggtcttt gataaattga gccaattctt ccaagaactc tggacggtat ttcttgataa
    29101 aggctgtttc gatgtagttg tgttcaatga ggtaattgat tttgtctttg attgaatcaa
    29161 aaaccatagt gtttggaact acattttctt taaagaaagc atccaaggct tccttatctt
    29221 tatgaagcat gatttgtcca ttaacaggac ggttaatttc gttattaaga cggaagtaag
    29281 tcacgtcttc aagatgtttt aatcccataa aatttccctt atctaattac aaaagaaagg
    29341 cttctaagtt agccctaaaa gcagtttctt ctggatgatg tactaagatt atgctaattg
    29401 tttcagtttt tctggttgga aacctgaaaa gacttcagtt ggtgtttgga taacaggagc
    29461 tgcgctaaaa ccgagctctt taacttgatc gacgtactca ggttgctcat caagattgat
    29521 ttcacgataa gagacattat tactgtccaa gaaacgcttg gtcattttac attggacaca
    29581 attgttttta gaataaacgg ttaccattgt gtaactcctc ttcaaaattt aatactatct
    29641 tagtatatca gaaaataaaa ttttgtcaat gatttaaact aaatatagtg gtgttctttt
    29701 gttgttttta tatataaaca caatatatag tgtttgtatt gtgaaatagc aatgattatc
    29761 ctataaattg tttttgaaga aactatatcc tgtgttttgt tatctaggat agaagatttt
    29821 cagcaagtta tctgaaagga aattgaagga aaatccagaa aatgcttgaa aaaaatccta
    29881 gaagatggta taatactaaa ttgtaagggt tatcacatat aactcaaaaa aagaaagaac
    29941 aaaaggagag tcaaactatg gcttctaaag atttccacgt agtggcagaa acaggtattc
    30001 acgcacgtcc agcaacattg ttggtacaaa ctgctagcaa atttgcttca gatatcactc
    30061 ttgagtacaa aggtaaatca gttaacctta aatcaattat gggtgttatg agtcttggtg
    30121 ttggtcaagg tgctgacgta actatctcag ctgaaggtgc agatgcagat gacgctatcg
    30181 ctgcaatctc agaaacaatg gaaaaagaag gattggcata agggaaatga cagaaatgct
    30241 taaaggaatc gcagcatctg acggtgttgc agttgcaaaa gcatatctac tcgttcagcc
    30301 ggatttgtca tttgagacta ttacagtcga agatacaaac gcagaagaag ctcgccttga
    30361 tgccgctcta caggcatcac aagacgagct ttctgttatt cgcgagaaag cagtaggtac
    30421 gctcggtgaa gaagcagctc aagtttttga tgctcactta atggttcttg ctgacccaga
    30481 aatgatcagc caaatcaagg aaactatccg tgcgaagaaa gtgaatgcag aagcaggtct
    30541 gaaagaagtt acagatatgt ttatcactat ctttgaaggc atggaagaca acccatacat
    30601 gcaagaacgc gcagcggata tccgcgacgt gacaaaacgt gtattggcaa accttcttgg
    30661 taaaaaattg ccaaacccag cttctatcaa tgaagaagtg attgtgattg cgcatgactt
    30721 gactccttca gatacagctc aattggacaa aaactttgta aaagcttttg taaccaacat
    30781 tggtggacgt acaagccact cagctatcat ggcacgtaca cttgaaattg ctgctgtatt
    30841 aggtacaaac aacatcactg aaatcgttaa agacggtgac atccttgctg ttaacgggat
    30901 cactggagaa gtgattatca acccgacaga tgaacaagcg gcagaattta aagcagctgg
    30961 tgaagcctat gcgaaacaaa aagctgaatg ggcacttttg aaagatgctc aaacagtgac
    31021 tgctgacggt aaacacttcg agttggctgc caatattggt actccaaaag acgttgaagg
    31081 tgttaacaac aacggtgcag aagctgttgg actttaccgt acagagttct tgtacatgga
    31141 ttctcaagac ttcccaactg aagatgagca gtatgaagca tacaaggctg ttcttgaagg
    31201 aatgaacggt aaacctgttg tcgttcgtac aatggatatc ggtggagata aggaacttcc
    31261 ttacttcgat atgcctcacg aaatgaaccc attccttgga ttccgtgctc ttcgtatctc
    31321 tatctctgag actggagatg ctatgttccg cacacaaatc cgtgctcttc ttcgtgcgtc
    31381 tgttcacggt caattgcgta tcatgttccc aatggttgcg ctcttgaaag aattccgtgc
    31441 agcgaaagca gtctttgatg aagaaaaagc aaaccttctt gctgaaggtg ttgcagttgc
    31501 ggataacatc caagttggta tcatgatcga gattcctgca gcggctatgc ttgcagacca
    31561 atttgctaaa gaagttgact tcttctcaat tggtacaaac gacttgatcc aatatacaat
    31621 ggcagcagac cgtatgaacg aacaagtttc atacctttac caaccataca acccatcaat
    31681 cctacgcttg attaacaatg tgatcaaagc agctcacgct gaaggtaaat gggctggtat
    31741 gtgtggtgag atggctggtg accaacaagc tgttccactt cttgtcggaa tgggcttgga
    31801 tgagttctct atgtcagcaa catctgtact tcgtacacgc agcttgatga agaaactcga
    31861 cacagctaag atggaagagt acgcaaaccg tgcccttaca gaatgctcaa caatggaaga
    31921 agttcttgaa cttcaaaaag aatacgttaa ttttgattaa tcgaaaagtc cctgcaactc
    31981 agttacaggg actttttttg atattttaaa aagaattttc aagaaaatct ttcttataaa
    32041 aagtccaacc ttgaaaaagt agtggtcaga acaaaaaata cttaaatggt tcataaaatt
    32101 cttgacaagt tggagattta ggagtaaact attaaccagt taagtaatag agaggagttt
    32161 ctgcaattta gaaatgaatt gcaactagaa atatcaaata gaaagagagt ttcgatgaaa
    32221 attaataaga aataccttgt tggttctgcg gcagctttga ttttaagtgt ttgttcttac
    32281 gagttgggac tgtatcaagc tagaacggtt aaggaaaata atcgtgtttc ctatatagat
    32341 ggaaaacaag cgacgcaaaa aacggagaat ttgactcctg atgaggttag caagcgtgaa
    32401 ggaatcaatg ctgagcaaat cgtcatcaag ataacagacc aaggctatgt cacttcacat
    32461 ggcgaccact atcattatta caatggtaag gttccttatg atgcgatttt cagtgaagaa
    32521 ttactcatga aagatccaaa ctataagcta aaagatgagg atattgttaa tgaggtcaag
    32581 ggtggatatg ttatcaaggt agatggaaaa tactatgttt accttaagga tgctgcccac
    32641 gcggataacg tccgtacaaa agaggaaatc aatcgacaaa aacaagaaca tagtcaacat
    32701 cgtgaaggtg ggactccaag aaacgatggt gctgttgcct tggcacgttc gcaaggacgc
    32761 tatactacag atgatggtta tatctttaat gcttctgata tcatagagga tactggtgat
    32821 gcttatatcg ttcctcatgg agatcattac cattacattc ctaagaatga gttatcagct
    32881 agcgagttag ctgctgcaga agccttccta tctggtcggg gaaatctgtc aaattcaaga
    32941 acctatcgcc gacaaaatag cgataacact tcaagaacaa actggctacc ttctgtaagc
    33001 aatccaggaa ctacaaatac taacacaagc aacaacagca acactaacag tcaagcaagt
    33061 caaagtaatg acattgatag tctcttgaaa cagctctaca aactgccttt gagtcaacgc
    33121 catgtagaat ctgatggcct t
//
LOCUS       DAVKLW010000022        32645 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKLW010000022 DAVKLW010000000
VERSION     DAVKLW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.022"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(332..1021)
                     /gene="rplA"
                     /locus_tag="VW324_000972"
     CDS             complement(332..1021)
                     /gene="rplA"
                     /locus_tag="VW324_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9726630.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1229..1654)
                     /gene="rplK"
                     /locus_tag="VW324_000973"
     CDS             complement(1229..1654)
                     /gene="rplK"
                     /locus_tag="VW324_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9726631.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(1777..2493)
                     /locus_tag="VW324_000974"
     CDS             complement(1777..2493)
                     /locus_tag="VW324_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9726632.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2511..2972)
                     /locus_tag="VW324_000975"
     CDS             complement(2511..2972)
                     /locus_tag="VW324_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9726633.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(2974..4011)
                     /locus_tag="VW324_000976"
     CDS             complement(2974..4011)
                     /locus_tag="VW324_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9726634.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(4109..5434)
                     /gene="brnQ"
                     /locus_tag="VW324_000977"
     CDS             complement(4109..5434)
                     /gene="brnQ"
                     /locus_tag="VW324_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9726635.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5605..5697)
                     /locus_tag="VW324_000978"
     CDS             complement(5605..5697)
                     /locus_tag="VW324_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9726636.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5740..5892)
                     /locus_tag="VW324_000979"
     CDS             complement(5740..5892)
                     /locus_tag="VW324_000979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9726637.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(6052..6630)
                     /locus_tag="VW324_000980"
     CDS             complement(6052..6630)
                     /locus_tag="VW324_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9726638.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6684..8084)
                     /gene="pepV"
                     /locus_tag="VW324_000981"
     CDS             complement(6684..8084)
                     /gene="pepV"
                     /locus_tag="VW324_000981"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9726639.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8097..8702)
                     /locus_tag="VW324_000982"
     CDS             complement(8097..8702)
                     /locus_tag="VW324_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9726640.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            8873..9673
                     /locus_tag="VW324_000983"
     CDS             8873..9673
                     /locus_tag="VW324_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9726641.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9857..10690)
                     /locus_tag="VW324_000984"
                     /pseudo
     CDS             complement(9857..10690)
                     /locus_tag="VW324_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(10680..12527)
                     /gene="uvrC"
                     /locus_tag="VW324_000985"
     CDS             complement(10680..12527)
                     /gene="uvrC"
                     /locus_tag="VW324_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9726642.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12567..13268)
                     /locus_tag="VW324_000986"
     CDS             complement(12567..13268)
                     /locus_tag="VW324_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9726643.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13347..14579)
                     /gene="murN"
                     /locus_tag="VW324_000987"
     CDS             complement(13347..14579)
                     /gene="murN"
                     /locus_tag="VW324_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9726644.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14584..15804)
                     /gene="murM"
                     /locus_tag="VW324_000988"
     CDS             complement(14584..15804)
                     /gene="murM"
                     /locus_tag="VW324_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9726645.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15916..16695)
                     /locus_tag="VW324_000989"
     CDS             complement(15916..16695)
                     /locus_tag="VW324_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9726646.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYMALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16764..18425)
                     /locus_tag="VW324_000990"
     CDS             complement(16764..18425)
                     /locus_tag="VW324_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9726647.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18600..20822)
                     /gene="recJ"
                     /locus_tag="VW324_000991"
     CDS             complement(18600..20822)
                     /gene="recJ"
                     /locus_tag="VW324_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9726648.1"
                     /translation="MITPTYEWQFALQVENADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21099..21858
                     /locus_tag="VW324_000992"
                     /pseudo
     CDS             21099..21858
                     /locus_tag="VW324_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21898..22662
                     /locus_tag="VW324_000993"
     CDS             21898..22662
                     /locus_tag="VW324_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9726649.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22675..23352
                     /locus_tag="VW324_000994"
     CDS             22675..23352
                     /locus_tag="VW324_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9726650.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFCIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23362..24021
                     /locus_tag="VW324_000995"
     CDS             23362..24021
                     /locus_tag="VW324_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9726651.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKIKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVPVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24317..25507
                     /locus_tag="VW324_000996"
     CDS             24317..25507
                     /locus_tag="VW324_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9726652.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCSPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(25828..26709)
                     /locus_tag="VW324_000997"
     CDS             complement(25828..26709)
                     /locus_tag="VW324_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9726653.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(26850..28178)
                     /gene="vncS"
                     /locus_tag="VW324_000998"
     CDS             complement(26850..28178)
                     /gene="vncS"
                     /locus_tag="VW324_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9726654.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(28175..28831)
                     /gene="vncR"
                     /locus_tag="VW324_000999"
     CDS             complement(28175..28831)
                     /gene="vncR"
                     /locus_tag="VW324_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9726655.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(28880..28963)
                     /gene="pep27"
                     /locus_tag="VW324_001000"
     CDS             complement(28880..28963)
                     /gene="pep27"
                     /locus_tag="VW324_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9726656.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(29143..30522)
                     /gene="vex3"
                     /locus_tag="VW324_001001"
     CDS             complement(29143..30522)
                     /gene="vex3"
                     /locus_tag="VW324_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9726657.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30574..31221)
                     /gene="vex2"
                     /locus_tag="VW324_001002"
     CDS             complement(30574..31221)
                     /gene="vex2"
                     /locus_tag="VW324_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9726658.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31234..32511)
                     /locus_tag="VW324_001003"
     CDS             complement(31234..32511)
                     /locus_tag="VW324_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726659.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9221 a         7487 c         6073 g         9864 t
ORIGIN      
        1 ctaaaacatt gttagaaatc gatttgactg tcctgaacga ttcgtcctat tcttatttca
       61 ttttattata attgatagtg gtcgccccag ccagatacct tatccactat ataggcctta
      121 ttatcacgtt cctgttcgta gagccacgat ttcgctatcc cttcctctga cgattcttat
      181 ttccagcgtc agacttggga atcgctattg ggttcaccgg tagtaggtgc ccaccgagga
      241 cttacacctc agatatacga catgcccgtc gtacatcaaa aaaagttgag acatagtgtc
      301 ttcaactttt ttacattact ttaactactg attaaagtga gtttacgtca actttgatac
      361 caacaccttg agtagttgtg atagtcaagt ttgttacgta agttccttta gctgtagctg
      421 gttttacttt ttggattgtt tcgttgaaag ctttaaagtt ttcaaccaat ttttcagctt
      481 caaatgatac tttaccgatg attgcttgaa cgttacctgc acggtcagca cggtaagtga
      541 ttttaccacc tttagactct tcaactgctt tagcaacatc cattgttaca gtaccagttt
      601 tagggtttgg catcaagtta cgtggtccaa ggacacgtcc aagacgtcca acaagagcca
      661 tcatatcagg tgtagcgata actacgtcga agtccaacca accgtcgttg attttagcaa
      721 caaggtcatc ttcaccaaca aagtctgcac cagcagcttt tgcttcttca gcttttgcac
      781 cacgtgcgaa aacaagaaca cgtgaagttt taccagtacc gtttggcaat accattgctc
      841 cacggatttg ttggtcagct tttttaacgt cgatgttcaa gttgtaagca acttctacag
      901 ttgcatcaaa ttttgcaaag ttagtttctt ttgcaagtgc tacagcttct tctacactgt
      961 atgcttttgt gctgtcgatt ttctcaagag cagcacgaag ttgtttgctt tttttagcca
     1021 ttttctattc tccttgtaag tggttcaatc gattttcatc tcccacgtca cttctcgaaa
     1081 tgatgaagtc ttgcgggttt tcagtctact atttttctct tcctacggcc ttaagctccc
     1141 ttgctgtatc caagtacaag cctcagtcac ttgcctagta tgaaaagcaa ctaatagact
     1201 gctgtgagat tttctactgt gttattgatt agtcaacaac agtgaatccc atagaacgag
     1261 cagtaccttc gatcatacgc attgcagact ctacgtttgc tgcgttcaaa tctggcatct
     1321 tagtttctgc aatttcttgt acttgtgcac gagtaactgt agcaacttta gttttattag
     1381 gtgtacctga tcctttttca acacctgcag cttttttcaa aagaacagca gctggtggtg
     1441 ttttcgtaac gaaagtaaat gatttatctt cgtaaactga gataacaact ggaatgatca
     1501 taccagcttg gtcagctgta cgagcgttga actcttttgt gaatcccatg atgttgatac
     1561 cagcttgacc aagagcaggt ccaaccggtg gagctggtgt agctttacca gcagggattt
     1621 gcaattttac aagtttttcg acttttttag ccatttttaa atcctccttt gtggttttgg
     1681 cggtaattag agatttttac ctcccacaag tatgctttac acataccttt ccattataca
     1741 ctatttatct aaaaatgcaa gagaaaactt tcatttttaa tcgacataat cgccgccttc
     1801 aaagccatag tactcttcca aactgagacc acttgcagca atttctttgg cttcttttcc
     1861 aacgtagcgg agatgccatt cttctgccat gtagccagtc tccttttcct tgcctttaag
     1921 atagcgaaca acaaagccat agtcagccgc atggtccaag agccactggg ctgccttttc
     1981 ttcagtaact aaatccccat ctgtcccaat caaatcaaaa gcaagacccg tctggtgttc
     2041 actgtaacct gggcgtgctg agtagcgatc tgctgcttcc ttaccatctt gattgacata
     2101 gtcttggtag agcttagcct gagtttcata actcctaaac ccactgtatt ggtcactgat
     2161 tggatagccc tcagcctgca tagctgcgat tagtttgagc aactctgcct tggctgttgg
     2221 attttctcct ggattgtaat cttttgacaa aggataacgt ttattagcta ggataatctc
     2281 atcgtatttc ccttggatac tataatagct ccctttattg ctaacttctg cctttttctg
     2341 actagaagct tgagatttgc tacctacagt tccttccggt tgactgcttt gctccgtttt
     2401 ttgagtgttt tcttcaactt tggttttttc ttgcgaacaa gctgcaaggg tcaatgctag
     2461 taaaccagat aaaatgacat atcttttttt catttctttc ttcctctctc ttaggccaaa
     2521 tacttctcca attcatgaga taagatttta atcatctctt ccagctctgg cgattgtact
     2581 tggcaatctt ctgccgccat ttcttcccag ggcacccacc agactgggcc acggccattc
     2641 aaaccgtgac tcttcatatc acctgctctt cggggaaaaa gatgccaatg agcgtgggca
     2701 tcgccatttc ctaggagttc aatattcatc ttttcagcct caaatgcttt ggcgactgct
     2761 tcttggacta aactcatttc ctctaaaaag cgaagtttta cagaagtttc catatgatgg
     2821 agttctgtga catgatcttt tgctaaaaat aaggtatagc ccttaaagta ttggtagtca
     2881 ccaataacaa catagcctgt ttctagttct tttacaaagt agggattttg cccagcttta
     2941 atgagttcga ttctctgaca gattaagcac attctatgat accgcctcgc tcttaagata
     3001 agcatcaacc atacgctcag tcgctattac tgaatcgatg tgggtgcgct cataagagtg
     3061 actggactca ataccagctc caagaagtgc atgcttgacc tctgcacctg ctgacatcgc
     3121 tgctgaagcg tccgatccgt aaaatggata gatgtcgagc ttaaatggaa tatcttgctc
     3181 tttagccaag gcaaccaagt gttgacggaa gtcatagtga taaggacctg aagcatcctt
     3241 gacacagata gatactgtgt actcatctgt ctgctggtca tctcccatag ctcccatatc
     3301 cacagccaga tattctacta cctgagcagg aatgttagaa ttagcaccat gtcctacttc
     3361 ttcaaaaact gaaaaagcaa aatgagttgt tactggcaat tcaatcttct ctttcttata
     3421 aatacgaagc aggttgagca aaatcgctgc gctgacctta tcatccaaat gacgagactt
     3481 gataaaacct gtatcagtca caacagttcg tgtatcgaag ctgataaaat caccgacctc
     3541 aatcccaaga gcacgagttt ctttttcatt gcttactttt acatccaaac gcacttccat
     3601 attgtcctgc gtgcgttctg cagttcctgc atccttgtag acatggcaag aagtttggtg
     3661 gatgaggatg gttcctgata ccttttgacc tgtgctagcc acatgaacgg tacagttttc
     3721 accttcaatc atgttccaag gaaaaccacc gatacggtcc aatttaagac gaccatctgg
     3781 tttgacagca cgaacaatag cacccagcgt atccacatgg gcagtcacat agcgatgttg
     3841 ctcatcattt tgacctttga tggtcacatt gacaccaccc ttagctgtgc gaactggctg
     3901 gtaaccaaaa ccttctagag tcttgactaa ataattcgaa atttcacgag tgaagcccgt
     3961 tggcgatgcg atggctgtca gttctttgat atattctact gtttgattca tttcttcacc
     4021 tctttcgcta cctattataa cacatttatc atcggataaa aaaagaaaat cactcctgta
     4081 gacttggcta caaaagtgat ttaagtgatt attccatttc aaaaacatcg ctttcttgct
     4141 tgtttggtag aaccaatgag agcaagattc caacaatggc tggcaccaac catgggagag
     4201 atgccttggc aaaaggaaga acgctaacaa gatttgcaag aaactcaacc ttaaacgagc
     4261 ttcctagtac gcttgcaatg gcaataactg taaccacagc aattgtcaac tgcatacctg
     4321 gttttgaaag ggcaacaaat ttgttgacaa tgacaatcat aacgatagca atcgtgattg
     4381 ggtacaagat aaccagtact ggaattgagt acttgataat cgcatcaaga cccaaattgg
     4441 caatagcaaa tccaatcaag gtaaaggctg tcgcataaac cttgtagctg atttgtggga
     4501 agcgctcatt aaagaactca gctgttgaca caatcaaacc aacagtcgtt gtgaagcagg
     4561 ttacggtaac catagctgca aggaagagtt gagctgttga gccaaagatt tcttgagtgg
     4621 cttgtgacaa gatgtaaaca cctggtgttc cacccttcat cgcttcagct ggtactggga
     4681 aatgatttcc aagaaaacct aaaccgatgt aaagagcgct gaaggcaagg gcaacaacga
     4741 taccaacaac ccaaatagtt gaaatgtatt ctttcttact tgaaaatcca agttgtttca
     4801 aggtttgaac tgcgattacg ctaaaggcca ctgaagcaag ggcgtccaag gtattgtaac
     4861 cttctaggaa acctgtacca aaggcagaag cttgataagc agctgaagca gcttgaggac
     4921 ttgttccacc atatttgata gctcccagaa cgaccaagat aacaatcaaa attgcaaaga
     4981 ctggcgttaa aacacgtcca atgcggtcta agatttttga tggattaagc gaaatcaaat
     5041 aggctgccgc aaaatacaga accgtaaata caatcaagcc aagtccttta tttgcatccg
     5101 acaaaagggg gctaatccct acttcgtaag ctgttgtagc agtacgtggg gtagcaaaga
     5161 atggaccgat tgacaagtaa agaactgaga ggtaaagagt cgcaaaccaa ggcgctatct
     5221 tcgttgaaat ctcgtagata tatcctttag gatttagcgt tccaataata agggtcaaga
     5281 cggcgatacc aacgcctgaa aagacaaaac ctgcgatggc aggaagaaaa tgttctccag
     5341 atagagcacc tagagaaggc ggaaaaatca agttccccgc accaaaaaat attccaaaca
     5401 ggagcaaacc tgttagggta ccttttttag ccataaaaat ctccttcata tttataaaat
     5461 actgacctag tataccacga ttaggagtct gaaaaaagca tcacgaggca aatattaaat
     5521 gttttcaatt ttcttaaaaa taagaattat ctaaaaataa aactctccta cccagtccac
     5581 accaagtagg agaaatgcaa aattttaaag ttcttcaaag gctaacatgc caccctggac
     5641 attgataaca ttataacctt gttctaacag gaattggcaa gcacgcgccg atctcattcc
     5701 agatttgcaa ataatataat gcaagcgatc tttgtccaat taatcataac tatcagccaa
     5761 ttgactaagc ggtaggttgt gggcacattc taaatgaaga gctgcaaact catccacttc
     5821 tctcacatcc actagagaaa gctggtcgtt ttggtaaagc tggtaaaatg cgtcaaaggc
     5881 tatttctttc attctttttc ttctaatact cttcgaaaat ctcttcaaac cacgtcagcg
     5941 tcgccttacc gtgcgtatgt taccgacttc gtcagtttta tctgcaacct caaagctgta
     6001 ctttgagcag cctacgacta gcttcctagt ttgctctttg attttcattg actataaaat
     6061 ggttttaatt ctttttttca aatctggcac tacttctgcc tcaaaccaag gatttttggc
     6121 catccagatt tgatttcgtg gtgatgggtg aactagcgga aaataggctg gcagatagtc
     6181 tttatagtgt ttcaccctct ccgttacctt cccactgatt ttctcctgta aatagtaggc
     6241 ttgggcatat tgcccaatca agagggttaa ctgaatatca ggcaattcct gtaagagctg
     6301 cggatgccat ttttctgcaa aacctgtacg aggcggaaga tcacccgact tgccatgtcc
     6361 tggaaagtag aaatccatag gcaaaacagc aaaataacct gaattgtaaa aggtatcttc
     6421 atccacacct agccagtccc gcaagcggtc accactttta tctttccagt aaaggcctgc
     6481 ttcttgagtt ttaagtcccg gagcctgacc gatgatattg atgcgagcag tttttggcgc
     6541 tgcaaagaga ggctcaatgc cacgctctgt atagctggca ttctgtgaat ccgccatgat
     6601 agcctgtttg attctttcga tttgagacat ctactttccc tccaaaaaga agtccaagca
     6661 taagatctca gacttctatc gttttatttg atcaattcgt aaatagcttc ggcatagatt
     6721 gctgctgctc ggaagagatc gtccaaggcg ataaattcat tggcttggtg catggtatca
     6781 attgagtctg ggaacatagc accataggca actccgcgct ctaacaagcg accaaaggtt
     6841 ccaccaccga tgacttgttc atgaccttta aagccagttt gtttttcata gatattcaac
     6901 aaggtttgca caagtggatc ttccattggc acatagtgag gcgtgtgacc gtgttcagac
     6961 aggctaacag aaacaactgg caagttttca aggattgact tgatttgttc tggacttgtt
     7021 ccttttggat agcggatgtt gagggcaatg gtattatcag cacttgtttc atcgaagcgg
     7081 aagacgccgg cattcataga aagagcaccc atcttttcat ccacatgagc aatcttaaga
     7141 ttttcaccct catgatcgtt caagagaatt ttacctgcga tgtcaaggta gtctttggct
     7201 ggaccagcaa agccaaactg actgaggaag agggcaaggt aagttgcgcc attgacacct
     7261 gaagcaggca tagcaccgtg ggctgattta ccaatgatcg tcaccttgta tttgccagct
     7321 tcttcttgga gttctcctct aagtttgtgt tctgcaacaa aggcatctag tttagcttgc
     7381 aagtcagcca agtcacctga aacgactgct gttgctgatt ctggtaccat attttcacgt
     7441 aaaccacctg taaagctgtg aagacgggca acacctgtat tttctcctgc aaagtggagg
     7501 tattccgtga tatttccttt ttcaccattg atgattggaa attcagcatc tggtgagaaa
     7561 ccgaaatctg gtttggcaag tcctacgtgc tcaaagtagt agtccatgtc tgcccagcct
     7621 gattcttcgt ctgttccaac gatgaagcga actttcttag aagttggaag acccaattct
     7681 ttgatgattt tcaaaccata gtaacaagct gttgtaggac ccttatcgtc cgaagccccg
     7741 cgcgcataaa ggcgaccatc tttgatagtt ggtgtgtaag ggtctgtgtc ccaaccgcta
     7801 ccagcaggca ccacatccat atgggcaaag attccgagaa cttcttctcc atcaccaaac
     7861 tcaaaatgtc ctgcatagtt atcaacattc ttagttgggt agccatcgcg gtctgcgatt
     7921 tcaaggaatt tctccaaggc ttttactgga ccaggcccaa atggatgctg ggcatcagcc
     7981 ttgctgtcat cacgttctga attgatttcc aaaaggctaa acaagtcagc caagaggtct
     8041 tctttgcgtt tttctacttc tgctgtaaaa tcaattgctg tcattttttc ttctttctat
     8101 cttttctcga tgatttcatc tactggcaag cggtagcttg gttccaattt ttcgtctgta
     8161 taacccactg tgatcaagag ttctgggcgg aaacggtctt cgatttccaa aacttcatta
     8221 acttttgatt tgtcaaaacc aagaataatg ttagaaccaa ttccttggtc tgtcaatgca
     8281 agaaccaagt tcatggcaac caaacctgca ttgagagcta ggtagtcgct gacttgttgt
     8341 tcattgtagc gtgcaaattc agctggcaaa tttttcatga agtattgaag ttgttcttct
     8401 gagaaattgt tagcaccacc aactcgggca atcttacgag cgcgtttggc aaggtctgta
     8461 tctgtaaata gggcaatggt tacaggtgct gatgatacct gctcaaagtt tgaaccataa
     8521 gccaactttg ccagttcagc atttttctca cgaacaacta caaatttcca aggctggctg
     8581 ttgtgggcgc ttggcgccaa ggttgcaatt tcgatagccg tacgcacatc ttttggatca
     8641 acaggcttat cagtgaaatg cttggtcgca tgacgttttt tatttaattc aagaaatttc
     8701 ataatcgatt tccttttcta attctactgc ttccattcta ccataatttg aaagagcttg
     8761 cagggatttt tcatgattaa gtttttctat cacagacata aaagatatat attggtttat
     8821 caggaaagtt tctgataaac tagcaagtac tttacatttg aatggagaca gtatgaaaaa
     8881 atttagccta ttactagcta tcctaccatt tttggttgcc tgtgggaatc aagctacacc
     8941 caaagagact agcgctcaaa agacaatcgt ccttgctaca gctggcgacg tgccaccatt
     9001 tgactacgaa gataaaggca atctgacagg cttcgatatc gaagttctaa aggcagtaga
     9061 tgaaaaactc agcgactacg agattcaatt tcaaagaacc gcctgggaaa gtatcttccc
     9121 aggacttgat tctggtcact atcaggctgc ggccaataat ttgagttaca caaaagagcg
     9181 tgctgaaaaa tacctttact cgcttccgat ttccaacaat cccctcgtcc ttgtcagcaa
     9241 caagaaaaat cctttgactt ctcttgacca gatcgctggt aaaacaacac aagaggatac
     9301 cggaacttct aacgctcaat tcatcaataa ctggaatcag aaacacactg ataatcccgc
     9361 tacaattaat ttttctggtg aggatattgg taaacgaatc ctagaccttg ctaacggaga
     9421 gtttgatttc ctagtttttg acaaggtatc cgttcaaaag attatcaagg accgtggttt
     9481 agacctctca gtcgttgatt taccttctgc agatagcccc agcaattata tcattttctc
     9541 aagcgaccaa aaagagttta aagagcaatt tgataaagcg ctcaaagaac tctatcaaga
     9601 cggaaccctt gaaaaactca gcaataccta tctaggtggt tcttacctcc cagatcaatc
     9661 tcagttacaa taacactctt cgaaaatctc ttcaaaccac gtcagcttta tctgcaacct
     9721 caaagctgtg ctttgagcaa cctacggcta gcttcctagt ttgctctttg attttcatat
     9781 tgagtataag atatattaaa agcgatggga tcagccaatc gcttttttag tttgcattgg
     9841 ttattttagg aaactcttac aaaaaatcaa aaaaattctt cacatcctct acatacctat
     9901 gcttttctat taagcttgct aatagttcca gattgtgcct ctgatagtta tcttcacgac
     9961 gtagttcttc atacatttcg ataaagggaa aacccttgga tttggcaaat tctaactcag
    10021 cctcgtaatc ataagcgact ttacgaaata ggatatttac caattcccca tcttcaacct
    10081 ctatcacggc atactgggca cggtgatttt ttaacgcctc ccaattaaaa tagggcatgc
    10141 caatcgaccc tggattgatg atttgttgcc cttgactacc ataacgaagc aactacttgt
    10201 gaacatgacc ataaactgcc acgtccgttt ccgcatctag cagttggtca aatttctctg
    10261 tatcattctc aactagcaag tcaccaccat agtttttgtc aggtaaatta tgagagatag
    10321 aaaagcgcaa tccgtcaatt tctttctttt ccagcaaagg caagcttcgt agccagacaa
    10381 tcgttgcagg atccattcgc tccatcaaat actgtgtcat acgcaagagc tgaacttcct
    10441 gtgggtcttc taagccatat tgcccatcta aagcctcaag gacacaatca tcccaattgc
    10501 ctcgaacact tgccgtgata ggaagttcct ttaacagagc gaccaagtca tttgcacctg
    10561 gaccagggag gaaaatgtct cccatgagcc aatattcact ggctccttga gttttagcat
    10621 ctgcaatcac tgcttctagc gccgtcgcat tgccatgaac atctgataaa attgcgattt
    10681 tatggtttat tgtggtttcc ttccgtttgg taatctactc tttcttccgc cacttgaagc
    10741 aagatttctg tttcctgcgg gttcaacttg gtttgcacag cctctgctac tgctcttggt
    10801 actccaactt cgacaatctc atccacactg gcttccttga ttttggtcag agacttgaag
    10861 tgcctcatga gattttgctt gcgtttaggt cccagaccat caatcccatc caattgagat
    10921 gagaaagaat ttttggagcg cagttggcgg tggaaggtaa tggcaaagcg gtggacctca
    10981 tcttgaatgc gttggaggag gaaaaattcc tgagaattgc gagacaagtc caccacttcc
    11041 agcggatctc caaagagcaa ttcatgggtt tggtgcttat cattcttttg cagacctgca
    11101 attggaatat ccaagcccag ttcttcttgg atgacttgct tagcaatatt gacttgacct
    11161 tgccccccat caatcacaat caaatctgga ggagtcaaag cctcacgctg tactcgacca
    11221 tagcgtctgc gaatgacctc tcgcatactg gcatagtcgt ctggcccgac tacggtcttg
    11281 attttatact tacggtaatc cttcttacta ggcttgccgt tgacaaagac caccatggct
    11341 gaaacaggac tagttcccat gatgttggag ttatcgaagg actcgatgcg aactggggtt
    11401 gggatttgaa gcaagcgtcc tagattttca atagctcctt gggtcttctc gacagatttc
    11461 tctagcaggt tgaacttctg ctctaggcta acacgggcat tttttatagc taaattgacc
    11521 agctgctttt tctctccacg ttgaggctta agaatcttgg aatccaccaa ggccttgaca
    11581 gcctcttcgt caatatcttg cggaatcagt acctcattgg gaactagatg ggatttttct
    11641 tgatagaatt gtcctacata ggtcaaaaaa tcctcatctg gatcattgaa gtaggggaag
    11701 agattgacat cgcgctcgat gagcttaccc tgacggacaa agaaaacctg cacacacatc
    11761 cagcccttat ccacatagta gccaaagaca tcgcgatttt ggagatcctt agccatgacc
    11821 cgttgcttgg ttcgaagcgt tccaatagcc tgaatcaggt cacggtattc cgccgcacgt
    11881 tcaaactcca tagtctgggc tgccgctgcc attttgccct ttagctcatc aatgattttg
    11941 ttatcctgcc ctttcagaaa atcagaaacc tcctgggcca tagacttgaa ataaatctca
    12001 tccttcttac agatggtgtg ggccatgcat tgaccgatat ggtaataaaa acagacctta
    12061 gagggcgggt tggtacactt acgaaaaggg aatatccgat ccagcaaccg cttgatttca
    12121 ttggctgccc ccacatcggg atagggtcca aaataaagac ctccgtcctt tttgacctga
    12181 cgagtgataa tcaagcgtgg atagcgctca ttggtgattt tgatgaaagg ataggacttg
    12241 tcatccttga gcatgatatt gtacttgggc ttgttttcct tgatcaggtt gatttctagg
    12301 agaagtgcct caatattaga ctccgtaaca ataaattcaa aatccacaat ttcagacacc
    12361 agagcctctg tcttggtatc atgacttcca cgaaaatagg accgtactcg attacgcaga
    12421 tttttagcct ttcctacata gataatggtg ccatttttat ccttatgaat gtagcaacca
    12481 gggctggtcg gcaagagctc tagttttgat ttaatcaagt tgttcatagt tccattatag
    12541 caaaaaaagt agggaaagat agtaccctac tcactagtct catatatttt tcggaggttt
    12601 gcaacatcct cttcctggat accataaaag gaacctgctg gttgcatgac agacttctca
    12661 tctgtatggt ccaagccaat cgcatgacct aactcatgtt ctgccgtatg gacaaggcgt
    12721 tcataggagt agccatagta tggattggac aaataataat gattcaaccg caccgttacg
    12781 gacaagaatt gccctgttaa gagattagtt tgactttccg cctctcctgc cacaggagtg
    12841 cctccgtcgt tcatctccgt agccgtaata tccgccttac tagactcagt cacgagttca
    12901 aagttaaaag caccagtttg attccagttc tggattgctt ctaaataagc ctcttgaaag
    12961 cgtgaatcca tctgcggatc caggtaaata cgagcagagg cctgtgccca ccttccttca
    13021 gaatgctggt gactatctgt ctggcttaac ttaggcagtt gccctgtttt ctcccattgg
    13081 tcaatacttt gttgaccaat ttttactgac cgttctgctt gctttagcgc tccttgaaaa
    13141 tctccgttca gataccagag aagtccgaaa gcaagcacac ataagagcac aactatccaa
    13201 accagacgcc aaaacaaacg ccacacaaaa gaaaagaaag cccctatcaa acgaaaaagc
    13261 cagcgcatgt ctctccacct ttctagcaaa taagaattat tttactaaaa gaagctaaaa
    13321 gaaatctaaa tactgacttt ttcatcttaa cgtcctacta tttttttgag taactggata
    13381 gctttgtatt ttaaaggaga tggatggtaa cggaaagtac ctgctttgcg tacaatatag
    13441 ccattaaaat tctgtttaaa acgcaaaaca ccatcacttc catcaaaaat cccttgaatg
    13501 cctaggaagt tgtatttagg tattccacgt tttatgcttt ccaacataac atatttttga
    13561 agcagggcag gggcatagaa cttattaaac tcagtgtagg aaccactaaa gagataagtc
    13621 gtttcctgag gcatataaac aaataaactc ccagctaaaa caatatcttc ttctccatat
    13681 ttttcaatca agtctcgcgc ttctgctttt cgaacttcaa acgtttcaaa ttgactagaa
    13741 tattctctca gttgattttg ttttttctca gaatgaggat ttttactcaa atcaagtcgc
    13801 aacttgtcca agttttcttc tagtttactt tgttcacctt gcaatttgct catatagtcc
    13861 gaaaaattta agcttgctat gagaaactcc gcttgttctc caaaagtatc ataaaaatgc
    13921 tcataatatt ctaaactttt atcactatat tctctacgtt cagaggtttc ttttgttata
    13981 ttcttaaaaa tcgatagttc ttcacgtttt aactttttca accgaatgcc aaaggtttca
    14041 gcctttttca ccaagggttt accctttttg ctaaaacttt taagcaaact cttttcagtt
    14101 aattcagtta aatctttata gtataaccaa tctggttctc cacctgggta acctgttgtt
    14161 aagccatcaa attgataacc taaatcagtc aaaccttgaa taatactttt tttctcagca
    14221 tctattggat taccttggct atcaaaagtt tgataagttt cataaggttt tacaagcaac
    14281 tctaatacac cattttgctt ggcatattct tttaactctg cataaaaaac tggaagagca
    14341 tcttgttggg tataaattgg ccccgaattg agttccatat gcagaccacc cagcatgggc
    14401 aagctataaa ccagagctgc aacttgaatt tctccttctt gtttcaaagc aagataaaca
    14461 attcgagccc ctcttttttc tagcaaatcc cccatctgga cagattgcat aaaggaacga
    14521 gaagaaacct gatcagaata agtctgaaac tcttctttcg tgagtgttgt tagtgccata
    14581 tacttacttt ctatgttttt ttcttaatgt tttacggaaa tcaagagcaa gtcttaacag
    14641 aggatagaga ggatgagtgg gcattgtaaa ttcacccaag tattcttcaa tcgttggatt
    14701 aaatttttcc ttaaaatgat aaagtccacc attgagagag ttttcaacac cacctaaatt
    14761 ttgccagacc atacctcgct caaaggcata gcgagccgtt tcataccatg ttaaaattgg
    14821 tgcattgtaa cgtttaaaat catcatccat accagcatat atattaacag aggtagtacc
    14881 aaattccaaa ctcaaagtag ccgctaaagg aactctcgct tgacctacat ctatatattc
    14941 ctgcaagaag gtcaattcct ctaacaaacg ttctttttcc ttcttctgcg cttctacttt
    15001 tgaagttcga gtcgactcag taaacgtctc ttccaaggct ctatttttcg ctaactgttc
    15061 ttctaattct tgcgaacgtt tagaaacatc caaggtggcc aaggtgatat aggccttgtc
    15121 cttaaaatta tctaacaatt ttttataata ggcttcattc ctcaaatgaa tctcttttcg
    15181 cttctcagtt tttttcatca actccgaaaa tgaatctaat agttccagtc caccatattg
    15241 aatctcaagc cctttgtttc gtgctgttcg aatagcctgt tttgttgact tggaaagttt
    15301 atcttcttca aaattttcct tgtatatttt cgcctgaata cgaggttgaa tggtgtctcc
    15361 catttcctcc gtttttcctg accaccttac tcccatttgt tgcaaactat caataatagc
    15421 cagattttca ggaaattctg tcttttcctg attgattaaa ctttgagata ggcaaatact
    15481 tgggtcaaaa gtcacaaaaa ccgctctctt actgcgagca taggacttaa tagactgaat
    15541 ggcaaaattc aagagttctt tatccccata atccaatata ggtcctcttg ggatgtaaaa
    15601 cattttatag cctagcggaa gagttctaat caaaatacta gctgtcgcca gtaatttttc
    15661 ttccctgtaa acaccaaact tctcatgttg ccaattagac ttaacttcct cccaagcact
    15721 actttgtaat acattggcta attcatgttc tttgacaaac tgatcatatt ctaatgtggg
    15781 aatgccaatt tgataacggt acatttccta ctctctttcc tccagtattt tattactatt
    15841 ctactacttt cgtttgaaaa gtctagtgaa aacatatttt attttagttc tactccccct
    15901 atgctgaagt tcaaactaag tcagtctctc ttctaatttg aaatcaattg gtagggttgt
    15961 taaaaaaact tccaattgtt tttcccagta gtaccactcg tgagttccag cactatggct
    16021 ataggtcaca tcaaaaccta gttttttgag atttttcact gcgagattat tggcttcgta
    16081 caagaaatcc tgttcgccac accacgccca aagtttggtc tttttatccg attttttagc
    16141 cagactttca agagaatagg gactagttgt ccagtctcta atctctccaa aaacacctct
    16201 ccagtaggct ggacttccca gattttgact ttcaggagaa aagttttgaa agctgagggc
    16261 acctgaaaaa ctagctgcat gagaaaaacg atttgtcgta agagccagtt tgaagcagcc
    16321 gtagcctccc atagaaagac cagcgataaa ggtcttttca cgcttgctag tcatattagg
    16381 gaagaagcgt ttcagaacct gtggcaattc ctctgctaga gccatgtagt agtcaaaacc
    16441 atactgggta tcggtgtacc aaccattgct ggtattgggc ataacaacga tgagattagt
    16501 tcctcgaagc aagcgttcta cattggtccg cttaagccaa ctattatgat ttccagacat
    16561 cccgtgcaaa aggtacaaga cgggaatatc ttcacactct ggttcttcca ctcgattggc
    16621 atcagggtag aggacattca ccccccactc catatccaat acttgtgagt aatactcgat
    16681 tttcattact gccatgattt tctccttcta tttttctata agaaaaagcc gaaactcgac
    16741 tttctctctg tttatttcaa cgattatttt gcttccatga ctactggtaa aatagctgga
    16801 cgacgcttgg tttggtcaaa gagatacttg gtcaaattat ctcgaacctt ccctttaaga
    16861 tctgcccagt caaagtcatc tccttgaaga tactcttcta ccgtttggtt aatcaattct
    16921 gaactttcac ggagaatatc gcgactcttc ttgagataaa caaatccgcg cgtatgaaca
    16981 cgagccctag ccacaatttt cttctcacga cggttgactg taatagccac gatgaaaatt
    17041 ccatcctctg acaagacctt acggtcacga agaacaacat ttccaacatc accaatggca
    17101 ttcccatcaa tcaagatatc tcctgctgaa accgatccag ctggaacaaa gtctccattc
    17161 tcgtaagcca tggtcgtccc ctttttagga atgaagatgc gttctggcaa catcccaact
    17221 gccatggcag ccttagcgtg agcatccaac tcacgatact ccccttggac agggaagagg
    17281 tacttaggtt gcaaaagatt gatcatcagc tgcaaatcac gcacatttcc gtgccctgat
    17341 acatgtaaac tttgggtaat caatttgaca accccacctg cctgataaat catattttcc
    17401 acacgcgcaa caaaggcttc tttagcaata gacggagccg tagcaatata gaccaggtcc
    17461 ccatccttga tttctacata acgatggcga ccaatcgaca tcttacgaag tccattgata
    17521 ggttcaccca tacgacctgt ctcaagaata atcaactcat ggtcttcaaa gcgagacata
    17581 tctttaggct taatcaagag aatttcgttg gctaaagaca acttcttaag acgaatcgct
    17641 gtgcggacga tattttcaat atcaaatcct gtcaagacga tacgtcgacc tgttttatcc
    17701 gcagcgtcaa aaatctgctg aatacgagaa agattactgg aaacagctgc aacgatgata
    17761 cgaccttccc agtcagcaat agtttgggta atttcatccc taacttcact ttcactagcc
    17821 acctgaatat tgctgtctgc attggccgaa tcactgagga gagccaggac gccgtcacga
    17881 ccaatctctg ccaaacgagc aaaatcagtt gcataagatt cactagccgt ttggtcaaat
    17941 ttgaagtcac ctgtataaac gatgcttcct tccgatgtct tcaagacaat tcccagactc
    18001 tctggaacgg agtaagtcgt agggaagaag gaaaccactg tcccaccaaa atcaatctcc
    18061 gtattctcat caatgacatg gaaatcatta aatttcttaa cggcatcatt tcctttgaca
    18121 aagagctttg ccaactcaat ggtcaactca gacccaaata caggaacttt agcctctgcc
    18181 aagagataag gtagagcacc aatggcatcc gcatgcccgt gggtcaagaa aaccccagca
    18241 atacggtcgc tattttcaaa aaggtaatcc atgtttggaa tcaccacatc gacccctaat
    18301 tgttcatttt caggatattt taaccctaca ttcaaaacaa aaatggactc tccaatttca
    18361 gcaatgtaca tattttttcc attctcacgc acaccaccaa gtgttgttaa actgatatta
    18421 ctcatttttc ctccaaaatc ttagtttatc ttgattatag ggcaactcta ccctcttatt
    18481 ctaaaagttc taatactttc agccgaatct tttcctacca ttataccatt tttttgatga
    18541 ttttcaaaat gaaggtctgt tttcaaaaaa gagttgattt cccaactctt tcctaacttc
    18601 tactcttttt ccatcaaaaa atcataaatt tcttgcaccg tacccagcgc catcatttct
    18661 tggtctttaa cggtttgttt gagattttgg taaatttgac tttctcctat ctcccgcttt
    18721 ggcgcctctt tattgactgt catcacacca tcttttatcg tcacaaagcc tagttcttca
    18781 aatacttgaa tcatcttgac cagcaagatt tgttgaatat taagatatgc agccaaatct
    18841 ttcagcttgt agcgaatatc aaactctggg aactggtaaa tagtcttgta caatttggca
    18901 aactgatctc tagtcccata acctgtcaga taataggcct tgtcaatatc atttttgaaa
    18961 tagacagcag agaaatgctg ttcctgaaaa atggtcttca gctgagtaat atcctctgga
    19021 atgtttttta cgacaacagc ttcactagcc gcaagatttg gcagttctcc aggaaaatcc
    19081 aagactggaa caccttctgg caagactgca ttttttccac gaatgttaaa aagttgaaca
    19141 ccttccactc gcgcatccat catcatcaac tggagggcag tttggccgtt ccattggttg
    19201 acagacaatt taaccgctaa ctctagattc ttggtttgag aaaactctgt cgcccatctg
    19261 ccttgaccaa aggctaccac ttcaaaactc acctcaccct tggaaatttt cagctttaga
    19321 tgggcattac ctgcccccat agtacgagca ctttcgacct gaaaattctt gatataaaaa
    19381 ataggtttct gattatccat tccaaaagga gctaaacgtt caaaactttt gaccgtttcc
    19441 aagctaagtg cctccaaatc caactcttca tctaggttta acttattctt gccaccagca
    19501 tctgcacctt tttcacgaac ataatcttcc aaaatctgag ataaatctga gagttgctca
    19561 acttccagcg tcatacccgc tgcacctgca tgacctccaa aggcgatgaa gaggtctcga
    19621 tagggatcca gagcttcaaa aatatcgacc gcttccacac tacgagcact gcccttggca
    19681 cgaccgtctt ctatattaag aacaatgact gtctgtccca attcttccaa taaacgacca
    19741 gccacgattc ctagaacccc aggattccag ccttccttgg ccaagacctg aaccttcttc
    19801 tcaggatcca ccatggtctt ggcttcttca tagatagact gaacgatttc cttgcgctct
    19861 tcgtttttct ggtgaatcat aagggcaatc tcatgcgctt cctcatcatc aaatccagtc
    19921 aacaaatcaa tggcaggatt gggatcatcc aagcgaccca aggcattcaa acgaggagca
    19981 atctggaaac caaccgtttc ttctgttact tcgttagcag caatcccagc catgtccagc
    20041 atttcttgca gaccaatgcg ctgggtatga cccaacattt ccagaccata ttgaactaag
    20101 atacgatttt catccgtcag actcaccata tctgcaatag ttccaatagc gaccaaatca
    20161 agcaattcca cttgcacttc ttctaacagg gcacaagcca acttgaaagc aactccacaa
    20221 ccagccaaat atttaaaagg ataatccgca tctggatgtt caggatggac aatagcataa
    20281 gcatctggca gggtttcagg tatggaatga tggtctgtca caatgacatc tactcccata
    20341 gactgagcca atgcaatagc ctcatgacca gcaaccccat tgtccaccgt cacaatcaag
    20401 gaaattcctt cttgctcgat aaagtattta taaacactag cattagggcc atagccatcg
    20461 gtaaaacgat ttggcaggta aactcggcac tccgcaccaa gttgttccaa actttccttc
    20521 acaatagaag ccgaagtcat gccatccgca tcatagtctc cataaacgag aatattttcc
    20581 ccttcttcaa tagcctgacg aatccgctcc actgccttgt ccatatcatg gagcagataa
    20641 gcatcatgta agtcctccaa ggaaggttct aaaaacttct tcagactttc ttggtcctga
    20701 atccctctct caaacaataa ccgagccacc tcaggaccca gtccagcctt cttggctatc
    20761 tttgtaaaat ccgcattttc tacctgcagg gcaaactgcc attcataagt aggtgttatc
    20821 aaaaagacat ccactctttc taagaattct attgcttcat tataacatga tttagacgtt
    20881 ttctctatcc agattgcttt tttataacat tttatagtat attgaaacta gaatagtaca
    20941 cctctacttc taaaatattg ttagaaatcg atttgattgt cccgatcgat ttttcctgtt
    21001 cttatatcac tatagtgaat tttttcagat atgatttgac aagaacattc ttttatcgta
    21061 gaatcatgtt ataaaatcta acacaaggga gatctctcat ggctttagta gaatttaaaa
    21121 acgtcgaaaa atattacgga gactaccacg cactccgcaa catcaatctc cgttttgaaa
    21181 aaaggacaag ttgttgtcct gcttggacct tctggctctg ggaagtccac tcttatccgt
    21241 acgatcaatg gtttagaggc tgttaacaaa ggaagtctcc tagtcaatgg gcaccaagtt
    21301 gctggtgcca gccagaaaga tttggtacct cttcgcaagg aagtcggcat ggtttttcaa
    21361 cattttaacc tttatccaca caaaacagtg ttagaaaacg taacacttgc acccattgaa
    21421 gttctaggaa ttgataaaaa agaagctgaa aaaaccgccc aaaaatatct ggaatttgta
    21481 aatatgtggg acaagaaaga ttcctatccc gccatgctat ctggtggaca aaaacagcgg
    21541 atcgccatcg ctcgtggtct tgctatgcat ccggaactcc tcctctttga tgaaccaaca
    21601 tctgctcttg atcctgagac tatcggagat gttctagcag ttatgcagaa actggcgcat
    21661 gatgggatga acatgatcat cgttacccac gaaatgggct ttgctcgaga ggttgcggac
    21721 cgcattatct ttatggccga cggagaagtt ttagtagata tgacagatgt cgatagcttt
    21781 tttgacaatc caagcgaacc tcgtgcccaa caattcctca gcaaaattat caaccacgaa
    21841 agtgacaaag tcaaataagg aggcgcctat gaaaaaaaat tctttttatc agcattattg
    21901 attagccttt tcggccttgt tgctgccaaa ccagtccagg ctaatactag tatcgcagac
    21961 attcaaaaaa gaggcgaact ggttgtcggc gtcaaacaag acgttcccaa ttttggttac
    22021 aaagatccca agaccggtac ttattctggt atcgaaaccg acttggccaa gatggtagct
    22081 gatgaactca aggtcaagat tcgctatgtg ccggttacag cacaaacccg cggccccctt
    22141 ctagacaatg aacaggtcga tatggatatc gcgaccttta ccatcacgga cgaacgcaaa
    22201 aaactctaca actttaccag tccctactac acagacgctt ctggattttt ggtcaataaa
    22261 tctgccaaaa tcaaaaagat tgaggaccta aacggcaaaa ccatcggagt cgcccaaggc
    22321 tccatcaccc aacgcctgat tactgaactg ggcaaaaaga aaggtctaac ctttaaattt
    22381 gtcgaacttg gttcctaccc agaattgatt acttcccttc acgctcatcg tattgatgcc
    22441 ttttccgttg accgctctat tctgtctggc tacactagta aacgcacaga actactagat
    22501 gatagtttca agccatctga ctacggtatt gttaccaaga aatcaaatac agagctcaac
    22561 gactatcttg ataacttggt tactaaatgg agcaaggatg gtagtttgca gaaactttat
    22621 gaccgttaca agctcaaacc atctagccat actgcagatt aaggaggaca ccccatgaca
    22681 gatttatcat cttggacagc ctattttcag gattttggac aatttttcaa tggtttcctc
    22741 tttactcttg ccctagcgat tggatccttt atcctcgcca tggtcttagg aatcttcttt
    22801 ggtgccatgt caaccagtaa acgtccaatt ttacgcattt tagctcgcat ctttgttgaa
    22861 ttttaccaaa atactcccct cttggtgcag tttgttattg ttttttatgg cctacctctt
    22921 atcagtgacc atatcatcat gattccaatt tattggacag ccgttctctg cgtgggactc
    22981 tatcacggcg cttatatcgc tgaggttatt cgttcaggga ttcagtctat tcctagtggt
    23041 cagatggagg ccgccttgtc gcaaggtttt acctatatca gtgccatgcg cttgattacc
    23101 ttgcctcagg ccttctgcat cattctacct ccattgacca accaaattgt taacctcatc
    23161 aagaacacct ctactgtagc tatcatctct ggagtagact tgatgtttgt gactaagtct
    23221 tggtcggctc tcaacggaaa ctatatccca gcctttttag gtgctgctct tctctacttt
    23281 gccctatgct tccctgttgc ccagtttggt cgcaagatgg agcaagccaa caaaaaagcc
    23341 tattcacttt aggaggttac tatggaatct attttagaag ttttaacccc agataaccta
    23401 gtctttatct ttaaaggatt tggcttgacc ctctatattt ctctgattgc catcatcctc
    23461 tctactatca tcggtacggt gctagctgtc atgagaaatg gcaaaaatcc tatcttacgc
    23521 attatttcca gtatttatat cgagtttgtg cgcaacgttc ccaaccttct ctggattttt
    23581 actatctttt tggtgttcaa aataaagtcc acaccagcag gtattacagc ctttactctc
    23641 tttacatcag cagccttggc tgagattatt cgaggcggtc tcaatgccgt agacaaggga
    23701 caatacgaag caggaatgtc acaaggcttc acctcagccc aaatcctcta ctacatcatt
    23761 ctcccacaag ccatccgcaa aatgctacca gccatcattt ctcagtttgt tcccgtgatt
    23821 aaggatacca gtctcctcta ctctgttatc gccctacaag aactctttgg agccagccaa
    23881 attctcatgg gccgttattt cgaaccagag caggtcttca gtctttacat cctgattgcc
    23941 ctcatctact tcagctttaa cctagcaatt tctagcctgt ctcatatgct agccaaacgt
    24001 tggcaacaag ctgcagaata atactcttcg aaaatctctt caaaccacgt ctgcttcacc
    24061 ttgccgtata tatgttactg acttcgtcag ttctatccac aacctcaaaa cggtgttttg
    24121 agcagcctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat aaacagccac
    24181 tctccagttc aattggagag tttttttgat gagagtagat atttatagac ctcaatttga
    24241 caccatacat tttctatgat aaaatgtaac aaatttattt acaacaaatc gaaatctaaa
    24301 tgaaatggaa ttttctatga aatcaatcaa aggactactc tttatcatag ctagttttat
    24361 cttgactctt ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt
    24421 aacaagccta tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt
    24481 tcacggtttg gagaaggtct acaccgtcca caaattcaca gcctttctct ccatcatcct
    24541 actaatcttt cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcagtt
    24601 tggcaatctt gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata
    24661 catccaatac gaagcttggc gatggattca ccgcctggtt tacctagcct atattttagg
    24721 actctttcac atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt
    24781 tcttgttggt agctatgccc ttttaggctt actagctggt ttttatatca tttttctata
    24841 tcaaaagatt tccttcccct atctagggaa aattacccat ctcaaacgct taaatcacga
    24901 tactagagaa attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc
    24961 ctttctaaag attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg
    25021 aggtcatggt caaactcttt actttactgt taaaaattca ggcgaccata ccaagaatat
    25081 ctatgataat cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat
    25141 catagaagaa ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt
    25201 catctcttac atccgtgaac atcctatttt agataaacgg gttcacttct actatagctt
    25261 ccgtggagaa gaaaatgcag tctaccttga tttactccgt gactatgctc agaaaaatcc
    25321 caattttgaa ctccatctaa tcgacagtac gaaagacggc tatcttaatt ttgaacaaaa
    25381 agaagtgccc gaacatgcaa ccgtctatat gtgtagtcct atttctatga tgaaggcact
    25441 tgccaaacag attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt
    25501 caaataagta atcatactct cctgggaaat cccaggagag ttttttatag ccaaatgaaa
    25561 taagacagga acgatggaat tttggcattc aaatcaattg ataagactat tttatagtgg
    25621 agtgaaatag gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt
    25681 agcagttatg ttatcctatt ctagtttcaa aacgctatag aagcagcatt gtactagtct
    25741 agattcagtt tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc
    25801 aggttttatc tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat
    25861 acgttcttca accgatgctt ggatagcttt tacaccgtca gccaagaatt tacgtgggtc
    25921 gaagagtttt ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg
    25981 agttgcgtta gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag
    26041 tttgatagct gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg
    26101 gaatcctgga agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt
    26161 tactgggtaa ggaccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc
    26221 aaccattgct ttagcgtctt cgattggagc caattcacct ttaccgatga ttccgtcttc
    26281 ttcaccaccg atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc
    26341 aacaacttct ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat
    26401 gattgaagta taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag
    26461 gtggatagct actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg
    26521 agcaactttg taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg
    26581 agctttttta gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt
    26641 aaatccacca actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac
    26701 gattgccatt ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca
    26761 ttttatcact ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct
    26821 aactccatct atgtaaaccc tttctctccc tagtcttggg cgacttttgg aaaagctata
    26881 aagaaggtta aacgattctc ctccatctcg aaacgataag ctaatttttc atgttctaat
    26941 agactcttaa ccacaaagag ccccatacca gaccccttga ccttgcgact ggcattgtca
    27001 gaaaaagact gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt
    27061 tctccttctc tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg
    27121 ctgatgagat tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg
    27181 gtcaaactat tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc
    27241 agattttgcg tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca
    27301 gaagagaggg aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca
    27361 actcccagat actggtctct atccttataa cgaccgatat tctctctcat attttcgatt
    27421 aggattttca aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg
    27481 accttcatct tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca
    27541 gtcaagagat gctggtagag gctattgatt tgttccttga gattacctat ctcatcctta
    27601 gaatccacgc gcaatcgcac ttgggaatcc aggtccatca tccgacgggt cacccgcttg
    27661 atttccaaaa tcggtgcaac aatagtccgg gcgtagatgt aggccaccaa gagggaaatc
    27721 agaaaggagg ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt
    27781 tgtaaatcca tggaagctag aaactggaga atcatagtac caccgtcttg cgttttcacc
    27841 tcgcgctcct caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc
    27901 ttgacttcta acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc
    27961 tgggaataca agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg
    28021 gcaatggctg ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga
    28081 aaaagaaaat aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta
    28141 tagataaata tctttgcaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa
    28201 cattgcgcac agtgaggata caatccaagt ctagcttttt ccgcaattcc ttgatataaa
    28261 catcaataac acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct
    28321 gagaccgagt caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt
    28381 tggcattgat aggcacttct tgacctgcga ggcttgcact gtagctttca aagtccacct
    28441 tggtatcctt gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca
    28501 ccctcacttt taagagggag agagagaaag gtttttccag atagccatct gccaaagagg
    28561 caaaggcact catcttgtat tcctcatcct gaaaagctgt caacatcaag acaggaacct
    28621 gactggtttt acgaatctca gctaggactt ctaagccgtt aagcttgggc atctggatat
    28681 ccagtaaaac cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg
    28741 ctgcctcaat agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca
    28801 tctcttcatc ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt
    28861 cttagaataa atacctactc tattttctat tatagtctct tgctggcctt ttgtctgtaa
    28921 gcaactgatc actagataaa acgttgtgaa attcctttct cataaattcc ataactttag
    28981 tatattatat ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac
    29041 tgctttcaga tttattttga attgttaaat agctattcct atccactatt cttgaataga
    29101 aacacaagat gcaatcttta ttccagactc attttttcaa atttattcac catccagcaa
    29161 gagctctttt ggttgttttc taaggagatt gcttgaagca agcgccataa cgagaaccac
    29221 tagaaccaag gcaaggacaa aaatgatgat aaagtctgat gtctgaatgg aaatgtctag
    29281 gctcgacaag gtcttgctaa agccatctac ttctgcaccg ccaccaaggt tagaggcttg
    29341 agccgcctta ctagcctgtt tggcaacacc tgaagtcaca ttggcaagga cagtgtttcc
    29401 aattgcacgg gcagtgtaat tagctaggaa gtaagcagaa actagagcag ggatagcaat
    29461 caagatagat tcggtgatga attgacccaa gatacttgcc tgcttgaggc cgatagagag
    29521 gagaattccc acttccttgc gacgggcgtt gatccaaagg ctgagcaaga gggcaaggag
    29581 gagaactgag aagctcaagc taccccagaa gaggaggttg gccatcttgt acataccaga
    29641 gatagattgc tcaagagctg ggtagttaga ggagctcttg acgagtgtgt agctcttcca
    29701 gttgatacca ctgatgccat tcaactcttt cataacatca tccaagttct tgtctgctgt
    29761 tacaaagaag gttgcgtccc cataaatggc tgtgtcttct gtgtatccat aaagttttgc
    29821 agcagtgtga atgtctgtaa tagctgtgtt ttcgtaaagt tcttgtgagt aggttactgc
    29881 tgacttatta tgaccatcaa agagtccctt gattgtcact tcaactgttt ccttggctcc
    29941 tttttcatta tctgcatcgt agatattaga gtccagttta accttgtccc ctactttcca
    30001 gccgtgtttg gctgccaagt ccttgtgcaa gaggatttta tccttgtcgt cgttggttaa
    30061 gtgctctcct tcgactagtt tataagaacc agagacaaac ttatcttctt tagaggagtc
    30121 attgacacct gtaatcatca agctacttcc aaaacgcttg gcacgatcag cagtgagatt
    30181 cttcttggtt tctggcgttt caatcaggtc atatccagtc aaatctccga tagcgttgat
    30241 acgtttgaca taagactcga tggccttgtt ttcggtgatt tttttgatgt cttcaccctt
    30301 gatattccca gcaccacgag gcgttccttg gttgacgcga cgattgattt gcatggagaa
    30361 gctattggtg atatttttaa aggtctcctg agaagccttg gcagtagctc ccttgattga
    30421 caagccgacc aaactcaagc tcgccatgag gagaataatc aggaagatga caatcgattt
    30481 gaaaaacttc cttgtaacat aggcaaatgc gttgtgtaac atagattccc tttctagatt
    30541 ttgttttaat cattctatca aaataagctc aaattattta ctagtattgc gcgtttcagt
    30601 cagtttctta tcctttaatt caagtgtaat atctgacgct tgtgccactt ctttactgtg
    30661 agttacgaca atcacacatt tacctgtttt ctgggcaagt gatttgagta gttcgacaat
    30721 atctccagca gttttaggat ccagatttcc tgttggctca tcagctagaa taactggagc
    30781 ttctgagacc aaactgcgag caatggcaac acgttgctgt tgaccacctg ataactggag
    30841 aacattccgc ttgatctggc tttcatccaa accaagctca agaagtgtat tcttgcttgc
    30901 ctttttgttg accaatcgga tattttccag cggagaaaga taatctatca agttataatt
    30961 ttgaaagacc agggaaatat ggtgcatgcg atggtaagaa tagcccttct tacgaatatc
    31021 ctctccttga aaaaggatag aaccttcaac aggactatct agaccagcaa gtagggacaa
    31081 gagtgtggat tttcctgctc ctgactcccc aataatactg taaaattttc cgggttcaaa
    31141 attataatcg atctgatata ggactgcttc agcagtattc ttataacggt aggtaacatc
    31201 ttgtaattgt aataaagtca tgatttctcc ttcttaacta atagatgata aaatttcttt
    31261 cggtgatttt ctaaataaga ataggaaaca aagggctaca gataagcaac taagcagaac
    31321 tagaaaaaca taggattctg caaaagataa gatactagtt gataaactgc ttgctttggc
    31381 tagtgtatct tgtaagcttg cctgatctcc acttgctagt agagtttgga gtaggtaagt
    31441 tgtgattccg tttcctgcaa caaatgctgg aagcaaagct ccaagagata ccaaaactac
    31501 ctctaaacag aattgtagga agatcgagct cttgcctttt ccaagtgcaa gtaaaatccc
    31561 cacttcatag acccgttctc tcaaccagag agacaaaacc agaattaagg ctccagctcc
    31621 tgctatcaac atcccataaa ggaagatggt caggaaggtt tggaaagttg caactgagtc
    31681 tttgatttgt tcaaaagcct tgttttcctt ttcgacttgg tagccttgat tttccaaggc
    31741 caagttttct acctgcttca tgagtccgtc catttcctta ggattttcta catagaagcg
    31801 tgctgcactg acttgagctt cactattgcc caaaagggtt tggctacttt catagtctgt
    31861 aaagacttga ttttcactga agtcagaaga caagcctgtg aatttctctt gttttttacc
    31921 agaaaagatg ccgataatct caaactctac tgtttgtcct tttccagatt cagactgacc
    31981 agcatccaag ccaatcttgt catgaagtga aagaccgttc ttcttagcca attcttcgtg
    32041 gataaggatt ttcttggaat cccctttttg aaggtgtcgc ccttctttta gattgaaagc
    32101 cgaactggta aaggttacat ccttggatga atcctcaaga gccgttaagc taaccaagtt
    32161 attgtctgca gctgataaat catcacgctc cacgctctgc tcgccagtca ctgcttcctt
    32221 gtcttttagt tttgcgaccg tctcaagttc aggagagaca ttttccagcc ccttaatctt
    32281 gcttacagat gctaggtctg acaacttgaa tgtctgacca ttctctatct tcttaataga
    32341 aaaagatgta ttgagtgatt tataaagatt gctttctact gttttgttgg acttcatcag
    32401 agtcaaacag gctgaaattc cggccaataa gaccaataaa atcagaaata aaataaaact
    32461 tctcagtcgc tttctgctga cataagccca agatctttgg attggattca tttgtcacct
    32521 ccatatttgt aagactatta taaaacccaa atatgaaatg tttatgaaat acgaaaaaaa
    32581 aatatcgagt aggggataat ctctagcccc tctcacacca ccgtacgtgc cgttcggcat
    32641 acggc
//
LOCUS       DAVKLW010000023        31611 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKLW010000023 DAVKLW010000000
VERSION     DAVKLW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.023"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(1..388)
                     /locus_tag="VW324_001004"
                     /pseudo
     CDS             complement(1..388)
                     /locus_tag="VW324_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            453..734
                     /locus_tag="VW324_001005"
     CDS             453..734
                     /locus_tag="VW324_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9726660.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            866..1810
                     /gene="manA"
                     /locus_tag="VW324_001006"
     CDS             866..1810
                     /gene="manA"
                     /locus_tag="VW324_001006"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9726661.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1832..3307)
                     /locus_tag="VW324_001007"
     CDS             complement(1832..3307)
                     /locus_tag="VW324_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU9726662.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3473..3637
                     /locus_tag="VW324_001008"
     CDS             3473..3637
                     /locus_tag="VW324_001008"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9726663.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3638..4144
                     /locus_tag="VW324_001009"
     CDS             3638..4144
                     /locus_tag="VW324_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEU9726664.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(4164..4619)
                     /locus_tag="VW324_001010"
     CDS             complement(4164..4619)
                     /locus_tag="VW324_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9726665.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4639..5814)
                     /locus_tag="VW324_001011"
     CDS             complement(4639..5814)
                     /locus_tag="VW324_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9726666.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5873..6718)
                     /locus_tag="VW324_001012"
     CDS             complement(5873..6718)
                     /locus_tag="VW324_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9726667.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6843..7400
                     /locus_tag="VW324_001013"
     CDS             6843..7400
                     /locus_tag="VW324_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9726668.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7419..7886
                     /locus_tag="VW324_001014"
     CDS             7419..7886
                     /locus_tag="VW324_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9726669.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7975..8604
                     /gene="upp"
                     /locus_tag="VW324_001015"
     CDS             7975..8604
                     /gene="upp"
                     /locus_tag="VW324_001015"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9726670.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8778..9368
                     /gene="clpP"
                     /locus_tag="VW324_001016"
     CDS             8778..9368
                     /gene="clpP"
                     /locus_tag="VW324_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9726671.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9447..9695
                     /locus_tag="VW324_001017"
     CDS             9447..9695
                     /locus_tag="VW324_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9726672.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9796..10956
                     /locus_tag="VW324_001018"
     CDS             9796..10956
                     /locus_tag="VW324_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9726673.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11224..12093
                     /locus_tag="VW324_001019"
     CDS             11224..12093
                     /locus_tag="VW324_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9726674.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12097..13053
                     /locus_tag="VW324_001020"
     CDS             12097..13053
                     /locus_tag="VW324_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9726675.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13053..13817
                     /locus_tag="VW324_001021"
     CDS             13053..13817
                     /locus_tag="VW324_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726676.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13817..14527
                     /locus_tag="VW324_001022"
     CDS             13817..14527
                     /locus_tag="VW324_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726677.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14941..15597
                     /locus_tag="VW324_001023"
     CDS             14941..15597
                     /locus_tag="VW324_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9726678.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15705..16800
                     /gene="prfB"
                     /locus_tag="VW324_001024"
     CDS             join(15705..15776,15778..16800)
                     /gene="prfB"
                     /locus_tag="VW324_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9726679.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16818..17510
                     /gene="ftsE"
                     /locus_tag="VW324_001025"
     CDS             16818..17510
                     /gene="ftsE"
                     /locus_tag="VW324_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9726680.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17503..18429
                     /locus_tag="VW324_001026"
     CDS             17503..18429
                     /locus_tag="VW324_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726681.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            18715..20895
                     /locus_tag="VW324_001027"
     CDS             18715..20895
                     /locus_tag="VW324_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9726682.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVADLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20947..21762
                     /locus_tag="VW324_001028"
     CDS             20947..21762
                     /locus_tag="VW324_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9726683.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21902..23245
                     /locus_tag="VW324_001029"
     CDS             21902..23245
                     /locus_tag="VW324_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9726684.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23460..24650
                     /locus_tag="VW324_001030"
     CDS             23460..24650
                     /locus_tag="VW324_001030"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9726685.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            25203..26138
                     /locus_tag="VW324_001031"
     CDS             25203..26138
                     /locus_tag="VW324_001031"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9726686.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            26172..27209
                     /locus_tag="VW324_001032"
     CDS             26172..27209
                     /locus_tag="VW324_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9726687.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            27350..27955
                     /locus_tag="VW324_001033"
     CDS             27350..27955
                     /locus_tag="VW324_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9726688.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            28113..28643
                     /locus_tag="VW324_001034"
     CDS             28113..28643
                     /locus_tag="VW324_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9726689.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            28664..29566
                     /locus_tag="VW324_001035"
     CDS             28664..29566
                     /locus_tag="VW324_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEU9726690.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            29566..30048
                     /locus_tag="VW324_001036"
     CDS             29566..30048
                     /locus_tag="VW324_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9726691.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            30050..31591
                     /locus_tag="VW324_001037"
     CDS             30050..31591
                     /locus_tag="VW324_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9726692.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
BASE COUNT         9613 a         5911 c         6900 g         9187 t
ORIGIN      
        1 tctcctgcct ctgtagcctt caaatgacgg gcaagaaagg ctttctcttc ctcaactgtc
       61 atatatgcat ggttacgacc accacgtgtt tcttgaagga gagagtcgag tccgaactcc
      121 tcatattttt tacattttgc taaatcgttg tttgattaca gtctaaaagc tctataatct
      181 ctttataaga tttgcccatc agacgaaata taatagattg aaactagaat agtacacctc
      241 tacttctaaa acattgttag aaatcgattt gtcctgttct tgtttcattt tactatagaa
      301 cgatttgaag gcgtttataa tatttagctg tacgagagtc ttttaaaagt gttttgatgg
      361 tttggatttc ttctttagtt gatttcatat tactattgta taatgctttt tgattttagt
      421 ttagtattat cgtagaaaat ggactcggtg ctatggatac accggatgag aatggttatg
      481 tagcagatga ctatcggatt acttacttag aggcccacat caaggccatg cgagatgcca
      541 tttaccaaga tggggttgac ttgcttggtt atacgacttg gagctgtatc gatccggttt
      601 cagctggcac aggggaaatg aataagcgct atggctttat ctatgtagat cgagataatg
      661 taggaaatgg gactctcaaa cgtagcaaga agaagtcctt ctattggtac atgtcattta
      721 tagcaatggt gtaagcatcg agtagagcac atttgtttac tatttttata aaatcttctc
      781 cctgctttat aagatgtaaa aaagagttca aatggaactc tttttttgct ataatgaggg
      841 gagaaaaatc agacaggaga ttaagatgtc agaaccatta tttttacaat cagttatgca
      901 agaaaaaatc tggggtggta ccaagttacg tgatgagttt ggctacgaca tcccaagtga
      961 aaaagtcgga gagtactggg ctatctcagc tcatctaaat ggtgtttcta aagttgccaa
     1021 tggtcgttac gagggaacag atcttgctac tttgtatgcg gaacaccgtg aattatttgg
     1081 caatcgtcca gaacctgtat ttccgctttt gaccaagatc ctcgatgcca acgactggct
     1141 cagtgtccaa gttcacccag acgatgctta tggactagag catgaaggcg aacttggaaa
     1201 aacagaatgc tggtacatca tcgctgcgga tgaaggttca gagattatct acggccacaa
     1261 tgccaagtca aaagaagaac tccgccagca aatcgaggat aagaactggg atgcgctgtt
     1321 gacaaaagtg ccagttaaag caggagattt cttctatgta ccaagcggta ccatgcacgc
     1381 tatcggggcg ggtatcttga ttcttgaaac ccagcagtct agcgatacca cttaccgtgt
     1441 ctatgacttt gaccgtaagg atgacaaggg caatttacgt gaacttcacc ttgaaaaatc
     1501 tatcgatgtc ttgaacattg gtgaacccgc aaatagccgt ccagtgacaa tcaaagcaga
     1561 tgatctacgt tccactctcc ttgtatccaa tgatttcttc gcagtttaca aatgggaaat
     1621 tactggaaaa gttaactttg aaaagacagc tgactacagc ttgttgagtg tcttggctgg
     1681 tcaaggtcaa ctaactgttg acgggaaaaa ctatccaatc caaaaaggga gacactttat
     1741 cctaccaagt gatgttgaag cttggactct ggaagggcaa gatttagaat taattgttag
     1801 tcatccataa aaaagaaagg gcctgagttc attactcaag tcctttttga ttacataaat
     1861 tgggcgatga agaccacgat gatgattgga atgacggaac gaagaaacga agaaggaaga
     1921 gccagacttg gaacagtcct tgtttccatg ctctttcatc gagatggagt tcctccattg
     1981 caagagcctt tttaaagata tagcctgtaa aaagtgaaag gtagagagct ccaaatggca
     2041 tgaggagatt gggaaccaag aagttcatag cgtcaaagaa ggtcttacca aaaatgtgaa
     2101 catccgccat gacaccgtaa gatagggctg aaggaatgcc aaagacaaag gtcaaaattc
     2161 ctaaaataac actccatttg gcacgtttgc tgttatcctg gttggtgata ttgcccacat
     2221 tgatctccag catcacgaca gaagaagtga cagtcgcaaa gaggaagagg acgtagaaaa
     2281 tggttccaaa aggcatcttg tcaaagagtt gaggcaagac gataaagagc aagctcggtc
     2341 ccccttcaga ctggatattg aagggggacc gagcttggaa aatggctaga cctgccatga
     2401 tggatatcga gatattcatg gctacgatgg agattcctga ctggactaga ttggttttct
     2461 tgtccaagta agaagcatag gtcaacatga ctgtaacccc tagtgagagg gcaaagaaag
     2521 attgtcccag agcatagagg agaccagcgc tagtcagttt tgaaaagtct ggtttgagga
     2581 agtaaagaac cccttccatg gcatttggca aactgagaga gcgaccgatg ataacaacaa
     2641 agatgataaa gagcaggggc atcatgactt tcgaagctct ttcaatccct ttttgaaccc
     2701 cacgtgatac aataaagata tttaataaga taaaggctgc ttgagctcct agggcaatgg
     2761 ctggatttga aatgattgaa gtaaataact gagcataatc acccgttcca ccaagttgga
     2821 acaatttccc aaactcaata cctagataga ctagaatcca tcctccgata acactgtaaa
     2881 aagataagag gataaagagg gcaaaggcgc caatccaacc gataaagttg tacttgttat
     2941 tcttgcccag ttttccaaag gttttgatag cggaaacgcc agcactacgg ccaagggcaa
     3001 actcagccag caggagaggg aaaccgatta aaatagtgga aatgagaaag attagtaaaa
     3061 agcctccacc gccattagca gcagtcatgt agggaaactt ccaaacggaa ccaagcccga
     3121 tgaccgagcc agcagatgct agaataaagc caagtttcga cccccattgt gatttttctg
     3181 acatagttaa actctgatct cctttttcaa aataagaaaa agctacttga gttgtcaaat
     3241 agccttctgt ttgattgata gacttgacta tcctattatg ttttctaagg tctgtcaaga
     3301 aaaccatttt caagttttca cacctttctc aaaaacgctt gactctgacc taaggggagg
     3361 ggttatacta tcattgtaag gaggaaatca tgtaccatat aaaagaagct gcgcagcttt
     3421 caggtgtctc tgtcaagacc ccgccctgca tcactatgac aagataggac tcttggtccc
     3481 cttaaagtcg gaaaacggct atcgaaccta tagtcaagag gatttggaac gccttcaggt
     3541 cattctttac tacaaatatc taggcttttc tttagagaaa atagcagaac tgttaaagga
     3601 agaaaggaca gatttattgc tccatttgac taggtagttg gactatctaa ctcgcgaaag
     3661 gcaacatctg gataccttga tttccacctt gcaaaaaact attcaagaac aaaaaggaga
     3721 aagaaaaatg accattgagg aaaaattcac gggatttagc tatcaagaca atcaaaaata
     3781 ccaccaagaa gcggtagaga aatatggtca agaagtcatg ggacaagcgc tcgaacgcca
     3841 aaaaggtcac gaagacgagg ctacggccgc ctttaaccaa gtctttcaaa ctttggcaca
     3901 aaatcttcaa gttggtttac ctgcaacagc aaccgaaaac caggagcaag cagccaagct
     3961 cttgcaagcc attcgcactt atggatttga ctgctctatt gaggtattcg gtcatatcgg
     4021 taaaggttac gtctacaacc cagagtttaa ggaaaacatt gacaagtttg gttctgaaac
     4081 agcccagtac acgtcagatg ccattgcggc ttacgttcag acaaatgcag aaaaaatagg
     4141 ctaggaattt cctagcctat tttttacttc aaatcataaa gccagtcgtc accgtttttg
     4201 tagtaaaaga attcactgag atcttcttct agaaacacac gaagcatatc agacatatca
     4261 tcggttgcaa gttttagatg agaaagattt tcaaagtcct cccaccaaac tttcccttcg
     4321 tctgaagact ggagttcacc agtaaagtgt tctgtcttgt aaaaaaggac gacataacga
     4381 taatccttgt cgtcatacca gtttttgata ccacagagtt ggggtttgga aatgatcaga
     4441 ccagtttctt ctttcacttc acgaatgaca gcatcgacaa aggattcgcc acgttcaaca
     4501 tgaccaccag gaaaagtaat gccagaccag tcgggactaa ctcggtcttg gaccaggacc
     4561 ttatctccgt ttttaatcat acacatgtta acaaattcga ctgcctctct tctgttcatt
     4621 cttcacaacc tttaatcttt aatcataatg cagacttccc gccacccagc cggtacagag
     4681 ggcagaagtg atgttaaagc cacccgtgtg ggcattgata tccataactt cgcctgcaaa
     4741 gtggaggcca ggtaccagct tactttcaag ggttttagga ttgatttcct tgagactgac
     4801 tccacccttg gtaacaaagg actttgcaag ggacattttt ccagttacag gaattttaag
     4861 ttctttaatg gactggacaa gttgttctcg ttccttttca gtcagttgtt tgactttttc
     4921 aggatatcct tgtacaaaaa attcggccaa gcgttctggt aacaaggttt ttaaagcgtt
     4981 tttcaaggat ttttcccgat tttcttctag aaatgtaacc aagtccttct cagaaagttg
     5041 aggcaaaaca tcgagtgaga gaacctcccc acctttgaca aagctagaca tgcgtagggc
     5101 agcaggacct gacaaaccaa agtgggtaaa gagtaaatca tgagtgatga catgcttacc
     5161 ataacttagg gtcacatcgt ccagagaaat cccttgtaag gctttatgtg gaaaatctgt
     5221 taataaagga ctttcagcag cctcaagatc ggtgatggta tgcttaaaat ggcgagcaat
     5281 ctcgtgacca aaaccagtcg aaccagtcga aggataagac ttaccacctg ttgtgacaat
     5341 gagtttctca caagtgaagg tttgatccgc tgacttaagg acaaactggt catctacttt
     5401 tttaacagaa acgatttcta tttgagtagc aacttgacca cctagttcgg tgattttctt
     5461 ttccaaagct tcgataatag tccgagactt gtcactggct ggaaagacgc gtccgtggtc
     5521 ttcgacctta agtttaacac cattttctgt aaaaaagttg atgatgtcat gattatcgaa
     5581 ctgggagaaa acactgtaaa gaaagcgtcc gtttccagga attccagcta gcaggttgtc
     5641 taagctacca ttgttggtca cattgcaacg tcccccacca gtcccagcta atttttttcc
     5701 aagtttccga tttttttcga tgaggagggt tttctgtcca taaaagctac tggaaatcgt
     5761 agccatcata ccagcaggtc ccccaccgat gacaatagta tcaaaatgtt tcatagctct
     5821 attgtaccac aaaaaaacaa gagatgatgg tcacctcttg tcaagaatgc aattaatcaa
     5881 tttcatagcc catcagcaaa ccgccctctt ctgcatagaa actgcagaga ccagaggttg
     5941 gtagaatttt aatatccgct tgtgggaagg tttcacggat tcgctctgag agctgttgac
     6001 aacatttttc gttattgcgt tgggccatga caatacggcc accagcatat ccagctttta
     6061 ctaactcatc ataggcagct tgaactgatt tctttgatcc ccttgctttt tgtagcaatt
     6121 cgagagttcc agtttcacta gcttttccga ccatacgaat gttgagaagg ccaacgaccg
     6181 taccgataag cttgctcaaa cggccgttct tcaccaagtt atcgactttg gctaggacaa
     6241 agagcaactt agttttttct tgataggcag tgatagcttc aaccacttct tcaaaagaca
     6301 agccctggtc aatcaagtca ttcaattttt ctacgagtag gtcaacttca ccaccagcag
     6361 ataaactatc aatcacatga atcttagtgt caggatggtc ttctagataa atattctttg
     6421 ctagttgagc actattgtga ctgccagaaa gggtacctgt gatggttact aggaaaatgt
     6481 ttttggcacc ttcaaatgct cgcaaatagt catctgggct tggacaagcc gattttgaag
     6541 cttctgcagt tgcatacatg gtttccatca tttggtcaat atcgagactg gcgtcatcaa
     6601 caaagacctg atcagctact tgaatggtta aggggacact tacaaaggtt gtgttaatag
     6661 ctggtgttgg cagttgacga taatcacaac cagagtcagc aataattttc caagtcatag
     6721 aaattctcca tctttgtcag ttatacattg acaaaggttc tgtctttttt tacaattata
     6781 tcatgaaaac ccttgaaaca aaagcatcat ccgtctgttg tgacaagtag aaagaaaatg
     6841 ttatgtctga acgtagaatc tctgaaaagt ctcttgaaaa tctcagaaaa tcaaaccaag
     6901 aatccaattt attaaccaga gaagccattg aaacagccct cttgcaactc ttggaaaaaa
     6961 aggagctgac caagattagt atttctgaat tggtcaaacg tgcaggtgtt tcgcgtgcgg
     7021 ccttttatcg caattatgac tccaaagagg aaattttaga gagcgtcttt aaacgaactg
     7081 tccacaatat tatggaacag ctgcatcatt acgatttaaa gacagacctt tatttggttt
     7141 gggttcacct tttccgggag gccagaaagg aagctagagt gattcaattg gccttggatt
     7201 accatctgga aaaaatcttt gtccaagcca tgcaggaatt tctagaaaaa taccatggga
     7261 aatcaaaagg tgtcagctct tatcttcatt ccttctggag ctcagccatc gtctctgtcc
     7321 ttctaaaatg gatcaaggat ggcatgaagg taccagctga aaagattgca gatttaggtt
     7381 taccattttt taaaaaatag agaaaaagga gaaaagagat gactgaaaaa agactggcct
     7441 gggatgagta ttttgcagcc caagccctac taattgcgaa tcgttccact tgtaaacgtg
     7501 ctaaagtggg cgcgattctg gtaaaagata ataaggttat ttccactggt tacaatggtt
     7561 cggtgtcagg gactgagcat tgtattgatc acgaatgttt ggtcattgaa ggccactgtg
     7621 ttcgcaccct tcacgctgag gtcaatgcta tccttcaagg tgctgagcgt ggtgttccca
     7681 aaggctttac agcctatgta acccattttc cttgtctcaa ctgtacaaaa caattgcttc
     7741 aggtcggctg caagcgcgtg gtttatatca accagtaccg aatggacgac tacgcccaat
     7801 acctttatca agaaaaaggg acagaattga cccatttacc acttgagaca gtacagacag
     7861 ctcttaaaga ggctgatcta atgtaaaaat tatcaaaaat aaatggctta gaaagatttt
     7921 taaaccgttt tttggtataa taagaagaat aaattgaaag aaggaattcc aaaaatggga
     7981 aaaattgaag ttattaatca cccactgatt caacacaaat tgtcaatctt gcgtcgtaca
     8041 gatacttcta caaaagcttt tcgtgagcta gtagatgaga ttgcaatgtt gatggggtat
     8101 gaagtacttc gtgatcttcc actagaagat gtggaaatcg aaacaccaat tacaaaaaca
     8161 gttcaaaaac aattggcagg taagaaattg gccatcgtcc caatcttgcg tgcaggtatc
     8221 gggatggttg atggtctctt gagcttggtt ccagctgcta aagttggcca catcggtatg
     8281 taccgtgatg aagaaacact tcaaccagtt gaatacttgg tgaaattgcc tgaggacatt
     8341 gaccaacgtc aaatttttat agtagaccca atgttggcaa caggtggctc agcaatcttg
     8401 gctgttgatt ctcttaaaaa acgtggcgca tcaaatatca aatttgtctg ccttgtatct
     8461 gctccagagg gtgtaaaagc ccttcaagaa gctcatccag atgtagaaat ctttacagca
     8521 gccttggatg aacgtttgaa cgaacacggt tatatcgttc caggtcttgg agatgctgga
     8581 gaccgcttgt tcggtacaaa ataagatcga aaagaagaat gacttggaaa agtatttcca
     8641 gtcacgaaag gaggttgggt ttttgtttct gtctaatgaa agcagagcaa aaatttgacc
     8701 ttttttgacc aagatattat aatagtctta tcttcagtca tttgaccaac aaaattaaaa
     8761 ctcaaaagga gaaatgaatg attcctgtag ttattgaaca aacaagccgt ggagaacgtt
     8821 cttacgatat ttactcacgt cttctcaaag accgcatcat tatgctgaca ggtccggttg
     8881 aagacaatat ggctaactct gttattgccc aattgctttt cttggatgcc caagatagta
     8941 caaaagatat ttacctttat gtcaatacac caggtggttc tgtttcagct ggtttggcaa
     9001 tcgtagatac catgaacttt atcaaggcag atgtccaaac cattgttatg ggaatggctg
     9061 catctatggg aacagtcatc gcatcaagtg gagcaaaagg caaacgtttc atgcttccaa
     9121 atgctgaata catgattcac caaccaatgg gcggtacagg tggtggtacc caacaaactg
     9181 atatggctat cgctgcagaa cacttgctca aaactcgtaa taccttggaa aaaatcttgg
     9241 ctgaaaattc aggtcagtca atggaaaaag tccatgcaga tgcagaacgt gataactgga
     9301 tgagcgccca ggaaacactt gaatatggct ttattgatga aattatggcc aacaattcat
     9361 tgaactaatg atgatagaag gcaaactcga ctgggtttgc tttttttggt ataataggga
     9421 gagatttctt agaaagagga tttatcatgt ttgaaaaagt caatcgctct ggcttgatta
     9481 tctatcttta ctataatcgt gatgccaaaa aactgcagga ttatggagat attacctatc
     9541 attccaagaa acatcgttac ttacaactct atgttccaac tcaagaagtg gagcaattgg
     9601 tcggacgctt gagcaaggaa aaatttatta aaaaagttag ggtttgtcat atccaagagt
     9661 tggaaacacc ctttgtgggc aatctttatc gataggaaaa cgttatcatc gaaaaagttc
     9721 aaggaaaaca ttgacaattt tctgataatt cggtatattc ttaacagact atttaagaaa
     9781 taaggagaca aaaagatgaa gaaaaaattt gccctatcgt ttgtggcgct tgcaagtgta
     9841 gcacttcttg cagcctgtgg agaagtgaag tctggagcag tcaacactgc tggtaactca
     9901 gtagaggaaa agacaattaa aatcgggttt aactttgaag aatcaggttc tttagctgca
     9961 tacggaacag ctgaacaaaa aggtgcccaa ttggctgttg atgaaatcaa tgccgcaggt
    10021 ggtatcgatg gaaaacaaat cgaagtagtc gataaagata ataagtctga aacagctgag
    10081 gctgcttcag ttacaactaa ccttgtaacc caatctaaag tatcagcagt cgtaggacct
    10141 gcaacatctg gtgcgactgc agctgcggta gcgaacgcta caaaagcagg tgttcccttg
    10201 atctcaccaa gtgcgactca agatggattg actaaaggtc aagattacct ctttattgga
    10261 actttccaag atagcttcca aggaaaaatt atctcaaact atgtttctga aaaattaaat
    10321 gctaagaaag ttgttcttta cactgacaat gccagtgact atgctaaagg gattgccaaa
    10381 tctttccgcg agtcatacaa gggtgaaatc gttgcagatg aaactttcgt agcaggtgac
    10441 acagacttcc aagcagccct tacaaaaatg aaagggaaag actttgatgc tatcgttgtt
    10501 cctggttact ataatgaggc tggtaagatt gtaaaccaag cgcgtggtat gggaattgac
    10561 aaaccaatcg ttggtggtga tggattcaac ggtgaggagt ttgtacaaca agcaactgct
    10621 gaaaaagcat caaacatcta ctttatctca ggcttctcaa ctactgtaga agtttcagct
    10681 aaagctaaag ccttccttga cgcttaccgt gctaagtaca atgaagagcc ttcaacattt
    10741 gcagccttgg cttatgattc agttcacctt gtagcaaacg cagcaaaagg tgctaaaaat
    10801 tcaggtgaaa tcaaggataa ccttgctaaa acaaaagatt ttgaaggtgt aactggtcaa
    10861 acaagcttcg atgcagacca caacacagtc aaaactgctt acatgatgac catgaacaat
    10921 ggtaaagttg aagcagcaga agttgtaaaa ccataataga aaaatgttga aatagggaat
    10981 gagcctttga ctcactccct gtttcgatat ttaatactct tcgaaaatct cttcaaactg
    11041 cgtcaacgtc gccttggatt atatatgtga ctgacttcgt cagtcttatc tacaacctca
    11101 aagcagtgct ttgagcaacc tgcggctagt ttcctagttt gctctttgat tttcattgag
    11161 tataagaacc tatcaaaaag tgagggaaac ccctcggaat tataaataga aagagtgaat
    11221 cttatgctcc aacaactcgt aaatggtttg attctaggta gtgtttacgc gctgttagcc
    11281 ctaggatata ccatggttta cggaattatc aagctcatca acttcgccca tggtgatatt
    11341 tatatgatgg gagcctttat cggttatttc ttgatcaatt ctttccaaat gaatttcttt
    11401 gtagcgctta ttgtagctat gctagcgaca gctattcttg gtgtcgtgat tgagtttctt
    11461 gcttaccgac ctttgcgcca ctctactcgt attgctgttt tgattacggc tattggggtt
    11521 tctttcctat tggagtatgg aatggtctat ctggttggtg ccaatacccg tgccttccct
    11581 caagcgattc aaacagttcg atatgatttg ggaccaatta gcttaacaaa tgtgcagtta
    11641 atgattttgg ctatttcctt gattttgatg attttgttac aagtcattgt ccaaaagact
    11701 aagatgggga aagccatgcg tgcagtatca gtagatagcg acgcggcgca attgatgggg
    11761 atcaatgtaa accgtacgat tagctttacc ttcgctttgg gttctgctct tgcgggtgcg
    11821 gctggtgttc tgattgctct ttattataac tctcttgagc ctttgatggg ggttactcca
    11881 ggtcttaaat ctttcgttgc cgcagtactt ggtggtatcg gaattattcc tggtgcggct
    11941 cttggtggtt ttgtgattgg tctattggaa acctttgcga ctgcctttgg gatgtcagat
    12001 ttccgtgatg ccattgttta tggaatcttg ttgttgatct tgattgtccg cccagctggt
    12061 atccttggta agaatgtgaa agagaaggtg taaacgatga aggaaaattt aaaagttaat
    12121 attctatggt tactcctttt gttagctggc tatagcttga ttagtgtact ggtttcagtc
    12181 ggagtactta atctattcta tgtacagatt ttacagcaaa ttggaattaa tattattttg
    12241 gctgttggtc tcaacttaat cgttggtttt tcaggacaat tttcacttgg tcatgctggt
    12301 ttcatggcga ttggtgccta tgcagcagct attattggtt ctaaattacc aacctacggt
    12361 gccttctttg gagctatgct tgtaggggct ttgctttcag gagcagttgc cttacttgtc
    12421 ggcattccaa ccttgcgctt gaagggggac tatcttgcgg tagcaactct gggtgtttct
    12481 gaaattatcc gtatctttat catcaatggt ggaagcctta caaatggtgc ggcaggtatc
    12541 ttagggattc ctaactttac aacttggcaa atggtttact tctttgtcgt gattacaacc
    12601 attgcaacct tgaacttctt gcgtagccca attggtcgtt caaccctctc tgttcgtgaa
    12661 gatgaaatcg ctgctgagtc agttggggtt aatacgacta aaattaaaat catcgctttt
    12721 gtctttggtg ccattactgc aagtattgct gggtcacttc aggcaggatt tatcgggtct
    12781 gttgtaccga aagattacac cttcatcaac tcaatcaacg ttttgattat tgttgtattt
    12841 ggtggactcg gttccattac aggtgcgatt gtttcggcta ttgttctggg aattttgaat
    12901 atgcttctcc aagatgttgc tagtgtgcgt atgattattt acgctttggc cttggtattg
    12961 gtaatgattt tcagaccagg tggactcctt ggaacatggg aactgagcct atcacgtttc
    13021 tttaaaaaat ctaagaagga ggaacaaaac taatggcatt acttgaagta aaacagttaa
    13081 ccaaacattt tggtggtcta acagctgttg gagatgtgac tcttgaattg aacgaagggg
    13141 aactggttgg attaatcggt ccaaacggag ctgggaaaac cacccttttc aaccttttga
    13201 ccggtgttta tgaaccaagc gagggaacag taaccctaga tggtcacctt ttgaatggga
    13261 aatcacctta taagattgcc tctttgggac ttggacgtac tttccaaaat atccgtctct
    13321 ttaaagattt aacagtttta gacaatgttt tgattgcttt tggaaaccat cacaaacaac
    13381 atgtttttac tagtttctta cgcttaccag ctttttacaa gagtgaaaaa gaattaaagg
    13441 ctaaagcttt ggaattgttg aaaatctttg atttagatgg tgatgcagag actcttgcta
    13501 aaaatctttc ctacggacaa caacgtcgtt tggaaattgt tcgtgccctt gctacggaac
    13561 ctaaaattct cttcttagat gaaccagcag caggtatgaa cccacaggaa acagccgaat
    13621 tgactgagtt aattcgtcgt atcaaagatg aatttaagat tacaatcatg ttgattgaac
    13681 acgatatgaa tctggtcatg gaagtaacag aacgtatcta cgtacttgaa tatggccgtt
    13741 taattgctca aggaactcca gacgaaatta agaccaataa acgcgttatc gaagcttatc
    13801 taggaggtga agcctaatgt ctatgttaaa agttgaaaat ctttctgtgc attacggtat
    13861 gatccaagca gtccgtgatg taagctttga agttaatgaa ggagaagttg tttcccttat
    13921 cggtgccaac ggtgcaggta agacaactat tcttcgcacc ttgtcaggtt tggttcgacc
    13981 aagttcagga aagattgaat ttttaggtca agaaatccaa aaaatgccag ctcagaaaat
    14041 tgtggcaggt ggtctttcac aagttccaga aggacgccac gtctttcctg gcttgactgt
    14101 tatggaaaat cttgaaatgg gagctttctt aaagaaaaat cgtgaagaaa atcaagctaa
    14161 cttgaagaag gttttctcac gctttcctcg tcttgaagaa cgtaagaacc aagatgcagc
    14221 tactctttca ggaggggaac aacaaatgct tgccatggga cgcgctctta tgtcaacacc
    14281 aaaacttctt cttttagatg aaccatcaat gggacttgcc ccaatcttca tccaagagat
    14341 ttttgatatc attcaagata ttcagaagca aggaacaacc gtcctcttga ttgaacaaaa
    14401 tgccaataaa gcacttgcaa tctctgaccg aggatatgta ctggaaacag ggaaaatcgt
    14461 cctatcagga acaggaaaag aactcgcctc atcagaagaa gtcagaaaag catatctagg
    14521 tggctaaaac aatccagtgg attgttttag tcggcagatg aagattacga agtaatcatc
    14581 attatagtct gggagactag tttaggttgc agataaagat tgcgaagcaa tcatcaaatc
    14641 cagtggattg ttttagtcgg cagatggaga ttacgaagta atcatcaata tagtccgggg
    14701 gaccttttta gtcggtagat tgagattgca aacaaatctg catctacatt gaaagcttaa
    14761 tttctaataa ttgaaaaaat cgaatgaaaa atttcttacc ttcattcaca gagctcgatt
    14821 tcagagctct ttttgctagc ttattcatac ttttctgaat ttcgaaaaag aaatgtaagc
    14881 gtttgataga tttacaaaaa gattgtataa tagggataag aatagaaaag gagaagtctc
    14941 atggcagtta aagattttat gacccgcaag gtagtttata ttagtccaga tacaacagta
    15001 tctcatgcag cagatttgat gagagagcaa ggtttgcacc gtctgcctgt tatcgaaaat
    15061 gatcaattag ttggtttggt gactgaggga accattgcac aagcaagtcc atctaaagca
    15121 acaagtcttt ctatctatga gatgaattat cttctgaata agacaaaagt aaaagatgtc
    15181 atgattcgcg atgttgtcac tgtctcaggc tatgctagtc tagaagatgc aacttatctg
    15241 atgttgaaaa ataagattag tattctccct gtcgtagata accatcaagt atacggagtt
    15301 attactgacc gtgacgtttt ccaagccttt cttgaaattg caggttatgg cgaagaaggg
    15361 attcgtgtac gctttgttac agaagatgaa gttggtgttc ttggaaaaat tgtttctttg
    15421 attgtagaag aaaatttgaa tatctcccat acagtcaata ttccgcgtaa ggatggtaag
    15481 gtgattatcg aagtgcaaat cgatggatca attgatttac cagccttgaa agaaaaattt
    15541 gaagcaaatg gtattcaagt ggaagaaatc gctcgcactt cagcaaaagt cttgtaagaa
    15601 gggaagccca aaggcttctt ttttcatgaa aaggggatta gagaaaaaga tggaaagaaa
    15661 tgataaaata tgctataatg aaataatgta aaaaaggagt atttatggac atttcagtaa
    15721 ttcgtcagaa aattgacgca aatcgtgaaa aattagcttc tttcaggggg tctctttgac
    15781 ctcgaagggc tagaggaaga gattgccatc ttggaaaaca agatgacaga acctgatttt
    15841 tggaacgata atattgcagc ccaaaaaacg tcgcaagaat taaatgaatt aaaaaacact
    15901 tacaatacct tccataagat ggaagagttg caggatgaag tcgaaatttt attggatttt
    15961 ttggctgaag acgagtcagt gcatgatgaa ctggtagcgc agttagccga acttgataag
    16021 ataatgacca gctacgagat gactctactc ttgtcagaac cttatgacca caacaatgcc
    16081 atcttggaaa tccatccagg ttctggtggt actgaggcgc aggactgggg tgatatgttg
    16141 cttcgtatgt atactcgtta tggtaatgct aaaggcttta aagtggaagt gttggattac
    16201 caagcaggtg atgaggctgg tattaagtcg gtaactttat catttgaagg gcctaatgcc
    16261 tatggtctcc tcaagtcaga aatgggtgtt caccgcttag tgcgaatctc accatttgac
    16321 tctgccaaac gtcgccatac ctctttcaca tctgtagaag tgatgccaga attggatgat
    16381 actattgaag tggaaatccg tgaagatgat atcaagatgg ataccttccg ttcaggtggt
    16441 gccggtggac aaaacgtcaa taaggtttca acaggtgtac gtttaaccca cattccaact
    16501 ggaattgttg tccaatcaac agtggatcgt acccagtatg gaaatagaga tcgtgccatg
    16561 aagatgttgc aggctaagct ctatcaaatg gagcaagaga agaaggctgc agaggtagat
    16621 tctctcaaag gtgagaaaaa ggagatcact tggggaagcc aaatccgttc ttatgtcttc
    16681 acgccttata ctatggtaaa agatcaccga actagctttg aggttgctca ggtagataag
    16741 gttatggatg gggacttaga tggttttatc gatgcttatc tcaagtggcg aattagctaa
    16801 gatagaaagg aactcacatg tcaattattg aaatgagaga tgtcgttaaa aaatacgaca
    16861 acggaacgac tgctctacgc ggtgtttcgg ttagcgttca accgggggaa tttgcttaca
    16921 tcgtaggacc ttcaggagca gggaagtcaa cttttattcg ttctctgtat cgtgaagtaa
    16981 aaatcgataa aggaagccta tcagttgctg gttttaatct ggttaagatc aaaaagaaag
    17041 atatcccgct tctacgtcgt agtgttgggg ttgtcttcca ggattataaa ttgttaccaa
    17101 agaaaactgt ctatgaaaat attgcttacg ctatggaagt aatcggggaa aatcgccgta
    17161 atatcaaaag acgagtgatg gaagttttgg acttggttgg attgaagcat aaggttcgtt
    17221 ctttcccaaa tgaactctca ggtggggagc aacagcggat tgcgattgcg cgtgcaattg
    17281 taaataatcc caaagtattg atagctgatg agccaacagg aaatctggat ccggataatt
    17341 catgggaaat tatgaatctt ttggaacgga ttaacctaca aggaacaact attttgatgg
    17401 cgactcataa tagccagatt gtaaatacct tgcgccaccg tgtcattgcc attgaaaatg
    17461 gccgtgtcgt tcgtgacgaa tcaaaaggag agtatggata cgatgattag tagatttttt
    17521 cgccatttat ttgaagcctt aaaaagtttg aaacgaaatg gttggatgac agtagctgct
    17581 gtcagttcag tcatgattac tttgaccttg gtggcaatat ttgcatctgt tattttcaat
    17641 acagcgaaac tagctacaga tattgaaaat aatgtccgtg tagtagttta tatccgaaag
    17701 gatgtggaag ataatagtca gacaattgaa aaagaaggtc aaactgttac aaataatgac
    17761 taccacaagg tatatgattc tttgaagaac atgtctacgg ttaaaagtgt taccttttca
    17821 agtaaagaag aacaatatga aaaattaacc gagataatgg gagataactg gaaaatcttt
    17881 gaaggagatg ccaatcctct ctatgatgcc tatattgtag aggcaaacac tccaaatgat
    17941 gtaaaaacta tagccgaaga tgctaaaaaa attgaaggtg tctctgaggt tcaagatggc
    18001 ggtgccaata cagaaagact cttcaagtta gcttcattta tccgtgtttg gggactaggg
    18061 attgctgctt tgttaatttt tatcgcagtt ttcttgattt caaataccat tcgtattacc
    18121 attatttccc gcagtcgcga aattcaaatc atgcgcttgg tcggagctaa aaacagttat
    18181 atccgtggac cgttcttgtt agaaggagcc tttatcggtt tattgggagc tatcgcacca
    18241 tctgttttgg tctttattgt ttatcaaatt gtttaccaat ctgtcaacaa atcgttggta
    18301 gggcaaaatc tatccatgat tagtccagat ttatttagtc cgttgatgat tgccctacta
    18361 tttgtgattg gggttttcat tggttcattg ggatcaggaa tatccatgcg ccgattcttg
    18421 aaggtttagg taaaatagct gcttttatga ggagattgta aaatctcctt ttttgctaca
    18481 agagtttttg aaaagagatg cgcagaagaa aagagcttcc aaagaagtcc cccagagaag
    18541 acttcttgat ggctaatttg aacttgaaat ttggcgcact agaaattttt tagaaaaaag
    18601 tgtaattttt ttataaaagc ctttacaaaa aaaattaaag tggtataatt aaatcataga
    18661 aagtgcaaac gttttcgtaa aacgtttgcc taaataaaaa taaaggagat ttgaatgatg
    18721 aaagatacat tcaaaaatgt cttgtctttc gaattttggc aaaaattcgg taaggctttg
    18781 atggtagtta tcgcggttat gccggctgct ggtttgatga tttcaatcgg taagtctatc
    18841 gtgatgatta acccaacctt tgcaccactt gtcatcacag gtggaattct tgagcaaatc
    18901 ggttgggggg ttatcggtaa ccttcacatt ttgtttgccc tagccattgg aggaagctgg
    18961 gctaaagaac gtgctggtgg tgctttcgcc gctggtcttg ccttcatctt gattaaccgt
    19021 atcactggta caatctttgg tgtatcaggc gatatgttga aaaatccaga tgctatggta
    19081 actactttct ttggtggttc aatcaaagtt gctgattact ttatcagtgt tcttgaagct
    19141 ccagccttga acatgggggt attcgtaggg attatctcag gttttgtagg ggcaactgct
    19201 tacaacaaat actacaactt ccgtaaactt cctgatgcac tttcattctt caacgggaaa
    19261 cgtttcgtac catttgtagt tattcttcgt tcagcaatcg ctgcaattct acttgctgct
    19321 ttctggccag tagttcaaac aggtatcaat aacttcggta tctggattgc caactcacaa
    19381 gaaactgctc caattcttgc accattcttg tatggtactt tggaacgttt gctcttgcca
    19441 tttggtcttc accacatgtt gactatccca atgaactaca cagctcttgg tggtacttat
    19501 gacattttaa ctggtgcagc taaaggtact caagtattcg gtcaagaccc actatggctt
    19561 gcatgggtag cagaccttgt aaaccttaaa ggtactgatg ctagtcaata tcaacacttg
    19621 ttagatacag tacatccagc tcgtttcaaa gttggacaaa tgatcggttc attcggtatc
    19681 ttgatgggtg tgattgttgc tatctaccgt aatgttgatg ctgacaagaa acataaatac
    19741 aaaggtatga tgattgcaac agctcttgca acattcttga caggggttac tgaaccaatc
    19801 gaatacatgt tcatgttcat cgcaacacct atgtatcttg tttactcact tgttcaaggt
    19861 gctgccttcg ctatggctga cgtcgtaaac ctacgtatgc actcattcgg ttcaatcgag
    19921 ttcttgactc gtacacctat tgcaatcagt gctggtattg gtatggatat cgttaacttc
    19981 gtttgggtaa ctgttctctt tgctgtaatc atgtacttta tcgcaaactt catgattcaa
    20041 aaattcaact acgcaactcc agggcgcaac ggaaactacg aaactgctga aggttcagaa
    20101 gaaaccagca gcgaagtgaa agttgcagca ggctctcaag ctgtaaacat tatcaacctt
    20161 cttggtggac gtgtaaacat cgttgatgtt gatgcatgta tgactcgtct tcgtgtaact
    20221 gttaaagatg cagataaagt aggaaatgca gagcaatgga aagcagaagg agctatgggt
    20281 cttgtgatga aaggacaagg ggttcaagct atctacggtc caaaagctga cattttgaaa
    20341 tctgatatcc aagatatcct tgattcaggt gaaatcattc ctgaaactct tccaagccaa
    20401 atgactgaag cacaacaaaa cactgttcac ttcaaagatc ttactgagga agtttactca
    20461 gtagcagacg gtcaagttgt tgctttggaa caagtaaagg atccagtatt tgctcaaaaa
    20521 atgatgggtg atggatttgc agtagaacct gcaaatggaa acattgtatc tccagtttca
    20581 ggtactgtgt caagcatctt cccaacaaaa catgcttttg gtattgtgac ggaagcaggt
    20641 cttgaagtat tggttcacat tggtttggac acagtaagtc ttgaaggtaa accatttaca
    20701 gttcatgttg ctgaaggaca aaaagttgca gcaggagatc tccttgtcac agctgacttg
    20761 gatgctatcc gtgcagcagg acgtgaaact tcaacagtag ttgtcttcac aaatggtgat
    20821 gcaattaaat cagttaaatt agaaaaaaca ggttctcttg cagctaaaac agcagttgct
    20881 aaagtagaat tgtaatatac ttgaggttgg aagctgtatt ccaacctctt attttgggag
    20941 aaaagaatga aatttttaac actcaatact cacagttgga tggagaaaga agcagaggaa
    21001 aaattccaga ttttgcttga agatattctt gaaaaggact atgatttgat ttgtttccaa
    21061 gaaatcaatc aggagatgac ctcgtcagag gtggaggtta atgaccttta tcaagctttg
    21121 ccagcagctg agcctattca ccaagaccat tatgttagac tcttggttga aaagttgtta
    21181 gagcaaggga aaaattacta ctggacctgg gcttataacc atatcggcta tgaccgctac
    21241 cacgaaggtg tggctatctt gtctaaaaca cctattgaag ccagagaaat tttggtttca
    21301 gatgtggatg atccaacaga ctatcatact cgccgtgttg ccctagctga aactgtagtc
    21361 gatggcaagg agctagcagt tgccagtgtt catctctctt ggtgggataa aggtttccaa
    21421 gaagaatggg cacgatttga ggctgtcttg aaaaaattga acaagccact tttactagct
    21481 ggagatttta acaacccagc aggtcaggaa ggttaccaag ctattttagc tagtccatta
    21541 ggcttacaag acgcatttga agttgctcaa gagaaaagtg gtagctatac tgttccgcct
    21601 gaaattgatg gctggaaagg gaacactgaa ccccttcgaa tcgattatgt ctttactacc
    21661 aaagagttag cggtggaaaa tttacatgtc gtatttgatg gtaacaagag tccacaagtg
    21721 agtgatcact atggcttgaa tgctatatta aactggaaat aataactgaa aagaggttgg
    21781 aactataaaa ttccagcctt ttcttactag agaagctact ggaaatagcc taaataagtg
    21841 agactactgt aatggaataa aatatggtat aattgataag atagatagaa tcgaggatgt
    21901 tatgtcattt acgaaatttc aatttaaaaa ctatattaga gaagccttga aggagttaaa
    21961 atttacaact ccaacagagg tgcaagacaa gttgattcct attgttttgg caggtcgtga
    22021 cctagtagga gaatcaaaaa caggttcagg taagactcat actttcttgt taccgatttt
    22081 ccagcaatta gatgaagcta gcgatagtgt acaagcagtg attactgcac cgagtcgtga
    22141 gttggctact caaatttacc aagtagcgcg tcagatttca gctcactcag atgtcgaagt
    22201 tcgtgtggtt aattatgtgg gtggtacgga taaggctcgc cagattgaga aattggaaag
    22261 caatcagcct catattgtta ttggaacacc aggccgtatc tacgacttgg ttaaatctgg
    22321 tgatttagct attcataaag ccaagacatt tgttgttgat gaagcagata tgaccttgga
    22381 tatgggattc ttggaaactg ttgataagat tgctggcagt cttccaaaag acttgcaatt
    22441 catggtcttc tcagcgacta tcccacaaaa actgcaacca ttcttgaaaa aatacttatc
    22501 aaatcctgtt atggagaaaa ttaagaccaa aacggttatt tctgacacca ttgataattg
    22561 gttgatttcg accaagggac atgataagaa tgctcaaatt taccagttga ctcagttgat
    22621 gcagccgtat ttggcaatga tttttgttaa cactaaaacg cgtgctgatg aattgcattc
    22681 atatctgact gctcaaggct tgaaggttgc aaaaatccat ggagatattg cccctcgtga
    22741 acgcaagcga atcatgaatc aggtgcaaaa tctggatttt gagtatattg tcgcaacaga
    22801 tttagcagcg cgtgggattg acattgaagg tgtcagccat gtcatcaatg atgccattcc
    22861 gcaagactta tctttttttg ttcatcgtgt tggtcgtact ggacgaaatg gcctaccagg
    22921 tacagctatt accctttatc agccaagtga tgactcggat atccgtgagt tggagaaatt
    22981 gggaatcaag tttagtccta agatggtcaa agacggggaa tttcaagata cctatgaccg
    23041 tgatcgtcgt gccaaccgtg agaaaaaaca agataaactt gatatcgaaa tgattggttt
    23101 ggttaaaaag aaaaagaaaa aagtcaaacc gggttataag aagaaaattc aatgggcggt
    23161 tgatgaaaag cgccgtaaaa ccaagcgtgc tgaaaatcgc gctcgcggtc gtgcagagcg
    23221 taaagctaaa cgccaaacat tttaatagaa attgttggag tattgagctc caactttttg
    23281 ctttatgaga acgaactatc taaaccgaaa cactacatta aagactgcaa attgcgatta
    23341 aaaatggtat aatgataaag ttatatagtc ccgataagat ggtaggtatt tattacgaag
    23401 agttttccta tcagtacttt gtaactctat aacaatattt tttaaggggg gacattttta
    23461 tgtcagaacg taaattattc acgtctgaat ctgtatctga ggggcatccg gataagattg
    23521 cagaccaaat ttcagatgcg attttggatg ctattttagc aaaggatcca gaggcgcacg
    23581 ttgctgctga aacagctgta tatactggtt ctgtccacgt ttttggtgaa atttctacaa
    23641 atgcctatgt ggatattaac cgtgtggttc gtgataccat tgcagagatt ggttatacca
    23701 atacagaata tggattttct gctgagacgg tgggagtaca cccatctttg gtggaacaat
    23761 ctcctgacat cgctcaaggt gttaacgaag ccttggaggt tcgtggaaat gctgatcaag
    23821 atccactgga cttgattgga gcaggtgacc aagggctcat gtttggattt gcagtagatg
    23881 aaacagaaga gctcatgcca ttgccaattg cactcagtca taaattggtt cgtcgtctgg
    23941 cagaacttcg taagtctgga gaaattagct atctccgtcc agatgcaaaa tcacaagtta
    24001 cagttgagta cgatgaaaat gaccgtccgg tacgtgtaga tacagtcgtt atttctactc
    24061 agcatgatcc agaggccact aatgaacaaa tccatcaaga tgtgattaac aaggtcatca
    24121 aagaagttat tccatcttct tatcttgatg ataagacaaa attctttatc aatccgacag
    24181 gtcgttttgt aatcggtggt cctcaagggg actcaggttt gactggtcgt aagattattg
    24241 tagatactta tggtggctac tctcgtcatg gtggtggtgc cttctctggt aaagatgcga
    24301 ctaaggtgga tcgttcagcc tcttatgcgg ctcgctatat tgccaagaat atcgttgcag
    24361 caggccttgc taagaaggca gaagtgcagt tggcctatgc tatcggtgtt gcgcaacctg
    24421 tttctgttcg tatcgatact ttcggtacag gaacagtagc tgaaagtcaa cttgaaaaag
    24481 cggctcgtca aatctttgac cttcgccctg cagggattat ccaaatgctg gacctcaagc
    24541 gtccaattta ccgtcaaaca tcggcttacg gtcacatggg acgtacagat attgatcttc
    24601 catgggaacg tttggataag gtagatgctt tgaaagaagc agtaaaataa gattttaaga
    24661 ggggaacgtc ctctcttttt tatagttttt aactatactg ggatactgtt ctgaaaatcc
    24721 attttgcgaa agtagagatt tacatgtata gtagattgaa actagaatag tacacctcaa
    24781 cttctaaaac attgttagca atcaatttga ctgtcctgat cgatttctcc tgttcttgtt
    24841 tcattttact atatttcttt aaaaatgata aaggttgaga tttctcctcg taatagataa
    24901 tcatggggat atttcaatcc aaagttttat tcgttatcac ttgactattg caaggttttc
    24961 tagagcaaca gagtcatgga atggactcat ggttgagatt tctccttgtt gcttggactt
    25021 cattcaaaag tctgttaccc aagccttgtt caaacttcca atacactagc tgttttcata
    25081 gcatgacttc tgtactagac tttcttttcc gaataaatag atagaaccac agaatctagt
    25141 aaacctagaa ttaaaattat ggtataatat tagcaataaa agaaatctgg aggattagaa
    25201 tcatggtatc aacgaaaaca caaattgctg gttttgagtt tgacaattgc ttgatgaatg
    25261 cagcaggtgt ggcttgtatg acgatagagg agttagaaga ggtcaaaaac tcagcggcag
    25321 gaacctttgt tactaagaca gcgaccttgg acttccgtca ggggaatcct gagccacgct
    25381 accaagatgt tccacttggt tccatcaact ctatgggctt gccaaataat ggcttagact
    25441 attatttgga ttatctttta gatttgcagg aaaaagagtc gaaccgaact ttcttcttat
    25501 ctctggtcgg catgtctcca gaggaaaccc atactatttt gaaaaaagtt caagagagtg
    25561 attttcgtgg tctgactgag ctaaatcttt cctgtccaaa tgttccaggt aaacctcaga
    25621 ttgcctatga ttttgagaca acagaccgga ttttggcaga agtgtttgct tacttcacca
    25681 aacctcttgg aattaaattg ccaccttatt ttgatattgt tcactttgat caagcggcag
    25741 ctattttcaa caaatatccg ctcaagtttg tcaactgcgt taactctatc ggaaacggcc
    25801 tctatataga agacgaatct gtcgttattc ggcctaagaa tggttttggt ggaattggtg
    25861 gagaatacat caaaccgact gctctagcca atgttcacgc cttttatcaa cgtttaaatc
    25921 ctcaaatcca aattatcgga acaggtggcg ttctgactgg tcgagatgcc tttgaacaca
    25981 tcctctgtgg agcaagtatg gtgcaggtgg gaacgaccct tcacaaagaa ggcgtcagtg
    26041 cttttgaccg cattaccaat gaactgaaag caatcatggt ggaaaaaggc tacgagagct
    26101 tagaagattt ccgtgggaaa ttgcgctata ttgactaaat taaatcgaaa aatctgaaga
    26161 aaggagagac gatgctagcc attgaagaaa gtcagaagtt gactttatca aatttaccga
    26221 gcctgagcct atttacaggg acagatcagg gtcagtttga agtgatgaag agtcaagtgt
    26281 tgaaacagat tgggtatgat tctgctgacc tcaactttgc ctactttgat atgaaagaag
    26341 tagtttacaa ggatgtggaa ctggagttgg tcagccttcc tttctttgcg gatgagaaaa
    26401 tcgtgatatt agattatttt atggatatca cgactgctaa gaaacgcttt ttgacagatg
    26461 atgagcttaa gtcatttgag gaataccttg acaatccttc tccaacaacc aagttgataa
    26521 tctttgcaga aggaaagctg gatagcaaaa gacggttagt caaattactt aagcgtgatg
    26581 ccaaggcctt cgatgcagta gaagtaaaag aacaagaatt gcgccagtat ttccaaaagt
    26641 ggagtcagaa acaaggtctg cagtttacca atcattcttt tgaaaatctc ctcatcaagt
    26701 cggggtttca atttagcgaa atccagaaaa atcttctctt tttacagtcc tataaggcgg
    26761 attctgttat cgaggaagag gatattgtta acgcaattcc caagaccttg caggacaata
    26821 tttttgattt aactcagttt attctgacta aaaagatgga tcaggcgcgc gatttggtga
    26881 gagacttgac cttgcaaggg gaagatgaaa tcaaactaat tgcagtcatg ctgggacaat
    26941 ttcggacttt tactcaggtg aagattttgg cggagtctgg ccaaacagaa tcgcagattg
    27001 caagtagttt aggtagttat ctgggacgta acccaaatcc ttatcaaatc aagtttgcat
    27061 taagagattc gagaggactt tctttgagct ttctgaagca agctatttcc tatttgattg
    27121 agacagacta tcagattaag acaggtcttt atgaaaaagg tttccttttt gaaaaggcac
    27181 tcttacagat tgctagtcag gtcaattgac atttgttgaa actacaaccc gcgtcaggat
    27241 tatcttgtcg tgggtttctt gctgatttgc ttatctataa gcaaaaaact tgaaaaaagt
    27301 gaatgtttgc gttatcattt tcatatagaa agaaaaagag gtattacaga tggctattat
    27361 cttaccagaa cttccatatg catacgacgc tttggaacca tacatcgatg cggaaacaat
    27421 gcatttgcac catgacaaac accatcaaac ttatgtcaac aatgccaatg cagctttaga
    27481 aaaacaccct gaaatcggtg aagaccttga agccttgctt gctgatgtag aatctatccc
    27541 agctgatatc cgtcaagcac ttatcaacaa tggtggcgga cacttgaacc acgctctttt
    27601 ctgggaattg atgactcccg agaaaacagc tccttcagca gaactggcag cagcaatcga
    27661 tgcaacattt ggttcatttg aagaattcca agcagccttc actgcagcag caacaactcg
    27721 ttttggttca ggttgggcat ggttggttgt caacaaagaa gggaaacttg aagtgacttc
    27781 aacagcaaac caagacacac caatctcaga aggtaaaaaa ccaatcttgg gcttggacgt
    27841 ttgggaacat gcttactacg tgaaataccg caacgtgcgt cctgactaca tcaaagcttt
    27901 cttttcagta atcaactgga ataaagtaga tgaattgtac gcagctgcta aataatgata
    27961 gttggaggga agaattgttc ttctcttttt taggttataa gattctggtc tgacaaaatc
    28021 gttagacttt tttcattttt tatgagaaac gtgctaactg ctagaaatta tggtagaata
    28081 aagtattaag taatcgtgga aaaaggatat ctatgcgaaa agaaattgca cctgaattat
    28141 acaactataa caagtttcct ggtccggagt tccatttaca cggggacaag gtcgaaacgg
    28201 aagggatagc tttttccttg gttgaaaata tcaaggatgc ctttgatgtg acgactttta
    28261 atcagcgttt ttcagaagtc ttaaccaagt ttgattatat cgtgggggac tggagcaacg
    28321 aacagcttcg cctacgaggt ttttacaagg atgaccgaac agaagaaaaa cttgaaaaaa
    28381 tcagtcgttt acaagactac cttttagagt attgtagtta tggttgtgcc tattttgtct
    28441 tagaaaatga agcccctaag cgagcatcat ttgacaagaa aatgcgtaag acggaagaag
    28501 aaacaccttc tagaaaagga aagaaaccga ctcaaactaa acgaaagtcg aatgcagata
    28561 agaaaaatag acgtcgtcag aaagaccagc attctcagaa agaggacaag gaacaacgtc
    28621 attttgtcat tcgtcagaag tgagtagaga ataaggagaa gaaatgaaac cctcaattca
    28681 tagtttggta catcaaacca tgcaggaatg ggtattggag cagggagaaa agaaattccg
    28741 tgcagatcaa atctgggaat ggctctaccg taaacgtgtg cagtcatttg aagaaatgac
    28801 caacctttcc aaggatttga ttgctaagct caatgaccag tttgtggtca atcccttgaa
    28861 acaacgtatt gtgcaagagt ctgctgatgg tacagtcaaa tatctctttg aattgcctga
    28921 cggtatgtta attgagactg tactcatgcg tcaacactat ggtttatctg tctgtgtgac
    28981 cactcaggtc ggctgtaata tcggttgtac cttctgtgcc tctggtttga ttaagaagca
    29041 gcgtgacctc aataacggtg aaatcgtagc gcaaatcatg ctggttcaga aatactttgc
    29101 tgatgaaggg gttcaggtca atcttgccgt gtctcttcac gcacccaaca atgaacttcg
    29161 ttcaagtatc atgaagatta accgtgcctt tccgattgaa aaactctttg ctgctattga
    29221 atactacatt gagacaacaa accgtcgtgt aacctttgaa tatatcatgc ttaatgaagt
    29281 caacgacggt gtagaacaag ccttggaatt ggctgaattg ctcaagaaca tcaagaaatt
    29341 gtcttatgta aacttgattc cttataaccc agttagtgag catgaccaat atagccgtag
    29401 tcccaaagag cgcgtgttgg ctttctatga tacgcttaag aaaaaagggg tcaactgtgt
    29461 ggttcgtcaa gagcatggta cagatattga cgcagcttgt ggacaattgc gctctaacac
    29521 aatgaaacgt gaccgccaga aggcagtcgc agcggtaaat ccttaatgat gaagaagact
    29581 tataatcata ttttggtctg gggagtcatt ttctatagca tttgcattgt ctgtttttgc
    29641 tttactcctc aagaacaatc taccgtggga gtgggaactc caggtattca gcatcttgga
    29701 cgcctggttt ttcttttgac tcctttcaat tctctctgga aactgggcga agtgagtgac
    29761 attggacaat tatgttggat ttttttacaa aatgtcctca atgtcttctt gttttttcct
    29821 ctgattttcc aactccttta tctatttcca aatttgcgga aaacaaaaaa ggtccttctt
    29881 tttagttttc ttgtgagtct tggaatcgag tgtacgcaat taatcttgga ctttttcttt
    29941 gatttcaatc gcgtctttga gattgatgat ttgtggacca atactttggg tggctatctg
    30001 gcttggctcc tttataaacg attacataaa aacaagataa ggaattaaaa tgagtatttt
    30061 agaagttaaa aatctgagtc acggttttgg tgaccgtgca atttttgaag atgtgtcctt
    30121 ccgtctcctc aagggagaac atatcggcct ggtcggtgcc aatggtgaag gaaaatcaac
    30181 ctttatgagt atcgtgactg gtaaaatgct gccagatgaa ggaaaggttg agtggtccaa
    30241 atatgtgacg gctggttact tggatcagca ctctgtcctt gctgaaagac agtcggtgcg
    30301 tgatgttctc cgtacggctt ttgatgagct tttcaaagct gaagctcgta tcaatgacct
    30361 ctatatgaaa atggctgaag acggcgcgga tgttgatgct ctcatggaag aagtaggaga
    30421 acttcaagac cgtctggaga gtcgtgattt ctataccttg gatgctaaga ttgacgaagt
    30481 agcgcgtgct cttggtgtta tggactttgg catggatacg gatgtaactt ctttgtcagg
    30541 tgggcaaaga accaaggtgc ttttggcaaa acttctcctt gaaaagcctg atatcttgct
    30601 gttggacgag ccgaccaact acttggatgc tgagcatatt gattggctca agcgctatct
    30661 ccaaaactat gagaatgcct ttgttctcat ttcgcacgat attccattcc tcaatgacgt
    30721 tattaatatt gtctatcatg tggaaaatca acagctgacg cgttactctg gtgactacta
    30781 ccagttccaa gaagtttatg ctatgaagaa atctcagcta gaggcagcct acgaacgcca
    30841 gcagaaagag attgcagacc tcaaggactt tgtggctcgt aataaagccc gtgttgcaac
    30901 tcgtaatatg gctatgtctc gtcaaaagaa attggataag atggatatta tcgaactcca
    30961 aagtgagaaa ccaaaaccat cctttgattt caaaccagct cgtacaccag ggcgctttat
    31021 cttccaagcc aagaacttgc aaattggtta cgaccgtcct cttactaagc ctttaaatct
    31081 taccttcgaa cgcaatcaaa aggttgcgat tattggtgct aatggtattg gaaaaacaac
    31141 tctcttgaag agtctcttgg gcattatctc gccaatcgct ggggaagtgg agcgtggaga
    31201 ttatttagaa cttggttatt ttgagcagga agtagaaggt ggtaatcgcc aaactcctct
    31261 tgaagctgtc tggaatgcct ttcctgccct taatcaagca gaagtccgtg cagcccttgc
    31321 ccgttgtggt ttgacaacca aacatattga aagccagatt caagtattat cagggggaga
    31381 gcaagccaag gttcgtttct gtctcttgat gaatcgtgaa aacaacgttt tagtgctgga
    31441 cgagccgacc aaccatttgg atgtggatgc aaaggatgag ctcaaacgcg ctctcaaaga
    31501 atacagggga tctatcctta tggtctgcca cgagccagac ttttatgaag gttggataga
    31561 ccaaatatgg gattttaata atttaactta agaatgagag taaaagaaat a
//
LOCUS       DAVKLW010000024        29178 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKLW010000024 DAVKLW010000000
VERSION     DAVKLW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.024"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(66..1688)
                     /gene="groL"
                     /locus_tag="VW324_001038"
     CDS             complement(66..1688)
                     /gene="groL"
                     /locus_tag="VW324_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9726693.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1704..1988)
                     /gene="groES"
                     /locus_tag="VW324_001039"
     CDS             complement(1704..1988)
                     /gene="groES"
                     /locus_tag="VW324_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9726694.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2161..2556)
                     /locus_tag="VW324_001040"
     CDS             complement(2161..2556)
                     /locus_tag="VW324_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9726695.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2634..3395)
                     /locus_tag="VW324_001041"
     CDS             complement(2634..3395)
                     /locus_tag="VW324_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9726696.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3428..4057)
                     /locus_tag="VW324_001042"
     CDS             complement(3428..4057)
                     /locus_tag="VW324_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9726697.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKKENG
                     ETVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEI
                     VEMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPG
                     ELRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4073..4390)
                     /locus_tag="VW324_001043"
     CDS             complement(4073..4390)
                     /locus_tag="VW324_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9726698.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4387..4686)
                     /locus_tag="VW324_001044"
     CDS             complement(4387..4686)
                     /locus_tag="VW324_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9726699.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(4971..5162)
                     /locus_tag="VW324_001045"
     CDS             complement(4971..5162)
                     /locus_tag="VW324_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9726700.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5323..5997)
                     /locus_tag="VW324_001046"
     CDS             complement(5323..5997)
                     /locus_tag="VW324_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726701.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTAKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6003..6449)
                     /locus_tag="VW324_001047"
     CDS             complement(6003..6449)
                     /locus_tag="VW324_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9726702.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6563..7066)
                     /locus_tag="VW324_001048"
     CDS             complement(6563..7066)
                     /locus_tag="VW324_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9726703.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISVACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7063..7425)
                     /locus_tag="VW324_001049"
     CDS             complement(7063..7425)
                     /locus_tag="VW324_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9726704.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7491..9356)
                     /locus_tag="VW324_001050"
                     /pseudo
     CDS             complement(7491..9356)
                     /locus_tag="VW324_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9366..9836)
                     /locus_tag="VW324_001051"
     CDS             complement(9366..9836)
                     /locus_tag="VW324_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9726705.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(9960..10676)
                     /locus_tag="VW324_001052"
     CDS             complement(9960..10676)
                     /locus_tag="VW324_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9726706.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11559..12974)
                     /gene="ply"
                     /locus_tag="VW324_001053"
     CDS             complement(11559..12974)
                     /gene="ply"
                     /locus_tag="VW324_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9726707.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(12985..13317)
                     /locus_tag="VW324_001054"
     CDS             complement(12985..13317)
                     /locus_tag="VW324_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726708.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13388..13996)
                     /locus_tag="VW324_001055"
     CDS             complement(13388..13996)
                     /locus_tag="VW324_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726709.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14009..14467)
                     /locus_tag="VW324_001056"
     CDS             complement(14009..14467)
                     /locus_tag="VW324_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9726710.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            14592..15045
                     /locus_tag="VW324_001057"
                     /pseudo
     CDS             14592..15045
                     /locus_tag="VW324_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15722..15982)
                     /locus_tag="VW324_001058"
     CDS             complement(15722..15982)
                     /locus_tag="VW324_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726711.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(16041..16592)
                     /locus_tag="VW324_001059"
     CDS             complement(16041..16592)
                     /locus_tag="VW324_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726712.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(16582..17577)
                     /locus_tag="VW324_001060"
     CDS             complement(16582..17577)
                     /locus_tag="VW324_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726713.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(17985..18410)
                     /locus_tag="VW324_001061"
     CDS             complement(17985..18410)
                     /locus_tag="VW324_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726714.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(18554..18922)
                     /locus_tag="VW324_001062"
     CDS             complement(18554..18922)
                     /locus_tag="VW324_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726715.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKK"
     gene            complement(18944..19150)
                     /locus_tag="VW324_001063"
     CDS             complement(18944..19150)
                     /locus_tag="VW324_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726716.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(19137..19298)
                     /locus_tag="VW324_001064"
     CDS             complement(19137..19298)
                     /locus_tag="VW324_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9726717.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(19656..20612)
                     /gene="lytA"
                     /locus_tag="VW324_001065"
     CDS             complement(19656..20612)
                     /gene="lytA"
                     /locus_tag="VW324_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9726718.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(20988..22358)
                     /locus_tag="VW324_001066"
     CDS             complement(20988..22358)
                     /locus_tag="VW324_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9726719.1"
                     /translation="MNKKRTVDLIHGPILLSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(22665..23831)
                     /gene="recA"
                     /locus_tag="VW324_001067"
     CDS             complement(22665..23831)
                     /gene="recA"
                     /locus_tag="VW324_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9726720.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(23886..25142)
                     /locus_tag="VW324_001068"
     CDS             complement(23886..25142)
                     /locus_tag="VW324_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9726721.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(25226..26242)
                     /locus_tag="VW324_001069"
     CDS             complement(25226..26242)
                     /locus_tag="VW324_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9726722.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(26250..26768)
                     /locus_tag="VW324_001070"
     CDS             complement(26250..26768)
                     /locus_tag="VW324_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726723.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEATEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(26758..27201)
                     /gene="tsaE"
                     /locus_tag="VW324_001071"
     CDS             complement(26758..27201)
                     /gene="tsaE"
                     /locus_tag="VW324_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9726724.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVVILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(27287..27907)
                     /locus_tag="VW324_001072"
     CDS             complement(27287..27907)
                     /locus_tag="VW324_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726725.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(28290..29153)
                     /locus_tag="VW324_001073"
     CDS             complement(28290..29153)
                     /locus_tag="VW324_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9726726.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIQAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
BASE COUNT         8736 a         6519 c         4991 g         8932 t
ORIGIN      
        1 tataaaaaga agggttagtc attccctcct tttgtgtttt ttataagttg ttttctatag
       61 aaagcttaca tcatcccgcc catcatactt ggatccattg ctggagctgg ggctactggt
      121 tctggtttat tggctacgac tgcttctgtt gtcaaaatca agctggctac agatgctgca
      181 ttttgtaggg ctgaacgact cactttaact ggatcaatga taccttgatc aatcatgtta
      241 acccactcgc cagttgctgc gttgaatcct ataccaagct cagcattttt caaacgatcg
      301 ataacgatag atccttcaaa tcctgcattg tgagcaattt gacgaacggg ttcttccaaa
      361 gcacggagaa caatattacg tcctgttgct tcatctcctg tcaattccaa ggtagcaaca
      421 gctggaatca cattggcaag agctgttcca ccacctgcaa caataccttc ttcaacagct
      481 gcacgagtag cgttgagggc atcttcaatg cggagtttca tttctttcaa ctcagtttca
      541 gttgcggctc cgaccttaat aaccgctaca ccacctgaca atttggccaa gcgttcttgc
      601 aatttttcac ggtcaaattc agaagttgta gtttcgattt gagacttgat aaccgcaaca
      661 cggttagaaa tcgcttcagg atttcctgca ccttctacaa taaccgtgct atctttgtcc
      721 acggtcactc tcgctgcttg accaagagct tcaattgtcg catctttcaa ctcaagacca
      781 aggtcttctg tgataactgt tccgcctgtt aagatggcga tatcttcaag catggctttg
      841 cgacggtcac caaaaccagg tgccttgact gctactacgt tgaaggttcc acgaatcttg
      901 ttcaaaacaa gagttggaag agcctcgcca tccacatcat ccgcaataat caagagtgga
      961 cgattgcttt ggagaatgct ttccaaaagt ggcaagattt cttggatatt ggaaattttc
     1021 ttgtctgtaa tcaaaatgta cggattttca aggtcagcca ccattttttc gctatcagtc
     1081 accatgtact gtgaaaggta accacggtca aactgcattc cttctacgac ttcaagctct
     1141 gtttccatac cacgtgactc ttcgatggtg atgacaccgt ctttgccaac tttttccatt
     1201 gcttcagaga tgtactcacc aactttttca gaacgagaag atacggctgc aacttgagcg
     1261 atagcttctt tattggcaac agggatggcg ttgtttttca aagcttcaac tgctgcggca
     1321 actgctgttt caatcccacg acgaataccg attggatttg cacctgctgt gacgtttttg
     1381 attccttcac ggacgatagc ttgggtcaag actgttgcag tcgtagtccc gtcacctgcg
     1441 atatcattgg ttttagaagc tacttctgat actaacttag cacccatatt ttcaaaatgg
     1501 tcttccaatt cgatttcttt ggcaatggtc acaccgtcat tggtaatcaa gggtgaaccg
     1561 aatgactttt caagaacgac attgcgacct tttggtccca aggttacttt aacagtgtct
     1621 gcaaggatat cgacaccacg aaccatggct gaacgggcat ctgatgaaaa tttaatttct
     1681 tttgacatac ttactttctc cttctattcc tcaatgattg ccaaaatgtt agcttcgcct
     1741 acgatgatgt acttttcatc gccatctttg acatcaagac ctgcgtgggc ttcaactaag
     1801 acacgatctc cagttttaac acttggagca accaagtcac cgttcaaggt acgaacacct
     1861 tgtccagtag ccacaacttg ggctgttttg gttttttctt gggctgaacc tgcgagaaca
     1921 aagcctccaa cagtttgttc tttttcttct acttttaaga ccacacggtc ccctaatggt
     1981 ttcaacatct gatttcctcc ataatctgat ttcctccata atgagataga tattattagc
     2041 actctttata cctgagtgct aattcatagt tctattgtat cacttagtca gaaataatca
     2101 agaaaaaagt ctgactttct caagataaaa agcctgagac caactcagac tttttaattc
     2161 ttaaaatggc aattcttcct cttccaagac caaatctgcc aaatcttggc ctgcattatt
     2221 ttcacgcata gcacgttggg cacgactttc caagagttgg aatcctgtca caaggacttc
     2281 ggttacataa ttcatctgac catttttctc aaagcgacgg gtacgcaatt ctccatcaac
     2341 ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc tgccccatag
     2401 aaccatattg acaaaatcag cttcacgttc accgttttgg tctttgtaac gacggttgac
     2461 agcgatagtt gctcgcgcta ctgacttgtc attgttggtt ttgtgcaatt ctggtgtaga
     2521 cgttaaacgt ccaatcaaga taactttatt atacatattt tcttcctcct acttatctat
     2581 tcgtaggaaa tcaaaaaaag ttacagaaat ttgtaacttt tcgagaaaat tttttatttt
     2641 ttatgaacca tgaaacctgt cgcctgttga ttggccataa tggtcatatc tgtaatctga
     2701 acacgacgag gttgactagt cacatagact actgtatctg caatatcctg agcttgcaaa
     2761 gcttctattc cttggtaaac ggacgcagct cgttctttat caccatgaaa acgcactgta
     2821 gaaaaatctg tttcgacaat tccaggctga atggtcgtca ccttgatatc cgttgcgatg
     2881 gtatcaattc gcagtccatc cgaaaaggtc ttaaccgctg ccttggtagc tgagtaaaca
     2941 gcggcaccag cataggcgta gattcctgcg gttgacccca tatttataat atgaccttga
     3001 ttgactttta tcattgctgg caagaaacag cgagtaaccg ccatcagacc tttaacattg
     3061 gtatccaaca tggtcaacat atccaactcc tcatagtctt gatagggagc caagtcaaga
     3121 gccagtccgg cgttgttgac caagatatca atcctcccta tcgtttctag aatatcagag
     3181 caaacagtct tcaccatgac catatccgtc acatctagtg gaaaagtcca gacggtttga
     3241 tttggaaagc ttactgcaaa ctccgacttg aggatttcta atctgtctat ccgtcgtcct
     3301 gttagaacga catcctcacc ctgctccaaa taagcacgcg caatcgcttc accgattcct
     3361 gaggttgctc ccgtaatcac tacatttttt gccatcttat ttccttctat cagatgctga
     3421 caagttccta ggcagtccag tgtttcgctg ggtcgaacgg tgttccgaca acttggtctt
     3481 ctgataattc aagcacccca cgtttttgcg gagcatttgg caaatgcaat tcacgaggac
     3541 tacacatcat cccaaaactc ttttcaccac gaagttcgcc tgggaaaatg aaattccctt
     3601 ttggcatcat ggcaccagga agagccacaa tggttttcaa cccaacacgc gcattaggtg
     3661 ctcctgcaac gatttgcact atcttgtcac ttgcgactgc aacttggcag atgttgaggt
     3721 ggtcactgtc tggatgagct accatctcga caatttcacc aacaacaaac ttaggttcct
     3781 tatcattaac aatttcttct gtaaaacctt ccgcctgtaa ttcttggttc aaacgagcga
     3841 cttgctcatc tgataaaaag acttgaccgc gctctgcaat ttcaaacaag ctcgaaacct
     3901 caaaaatgtt ccaagctact gtttccccat tttctttttt gaggaaaaca cgggctacct
     3961 tgcctttgcg ttccacattc agtttggcat ctccgctatt tttcacgatg accataagga
     4021 catcaccgac atgttctttg ttatacgtaa aaatcattgt ttcctttttc tcctatttca
     4081 atcctgctaa aaaatcgtta atttgctcct tacttttacg gtcgcgattg acaaaacgac
     4141 cgatttcctt gtccttttct agaacaacaa ggctaggaat tccgtaaaca tcccagagtt
     4201 tggccaaatc catatactga tctcggtcca ttcgaataaa ggtgaactct ggattggtct
     4261 cctcaatctc tggtaaggca ggataaatat aacgacaatc gccacaccag tctgccacaa
     4321 aaaggaagac cttcttgccc gctttttcca ctaaagatgc taattcttct aaacttgctg
     4381 gctgtatcat aagacttcct cctcatagac taggtcttca ttttcataga caaaggtata
     4441 atgacggcca tcctcaaaaa tgacgccacc aaccaagctc tccagactgc tttcgtaaac
     4501 ttgaacatag agggtcgcaa tttcccccat gtcggaaaaa tggtctcgca caatctctgt
     4561 caactcttcc tgagtcttca tgagcttacg gtcatctgca acttttttcg tggcaagagc
     4621 aaggcttccg atacctagca gagccaagcc tgccatccac atttttttag ctttcatacc
     4681 attcatttta acacaaaaaa ggcttcagga caaatgagga agcagcagaa aagcaagtaa
     4741 aaagcctctt cctttaagga aaaggacttc ttatactaag accaaactag aaagctagcc
     4801 gcaggctgct caaaacacgg ttttgaggtt gtagataagg cgacgctgac gtggtttgaa
     4861 gagattttca aagagtatta ttcttattgc caggcaccta agttgccaac gtagtaacca
     4921 tcaggtgtgt aggtattgcg aaacatctta cctgatgaag ccagataata ctacttgcca
     4981 ttgtctttga cccaatcatt cgcaatcatg gaaccagaag aacttacata ataccattct
     5041 cccttgtcat aaacccaagt actgactttc atggttcctg agcaattaaa ggcaaaaaaa
     5101 ctgtccaata tcattcgttt tttaaaagca tttgacacta catcatctac gctgtagtac
     5161 atagatacat caaacgtaaa agccaaaata tcgaaaaata tacaactttt aaaggcagca
     5221 tttcattttt taaagtctaa caaccataga actgaaatca gagcaaggca tcaatttcct
     5281 actcttagta cgtctatttt gatggttgaa gtgaagattt ttttatttaa cgtagacaat
     5341 ttccaacttc ccaaaagcca catctccacg gatagtcaag gttttgctgg ttggggctac
     5401 aggagcgtca gccttagctg caccaaagga ggtttctact ttcaaatcta cacgccagtg
     5461 ttgtggaaca tagacggttg catttccgaa ggccacttca atatttaaag ttgcaaaatc
     5521 acctagcatc tctgcattat catagtagat tttagcatcc ccaaaagcga cttccacttg
     5581 gtcatctacg agatcttgat cttgcttata gaaggtcccg ctaccaaaag cgacttcctt
     5641 agccgtgacg actgtttttt tcccattgta ccaccatttt ttttcattcc agaacttact
     5701 tgaatgcgtc agataaccaa caccaagtac caccaagatg ctagcccaaa taagagaatg
     5761 attggtaact ggtaacaagt cataagcgta atttgcaatg atgagcgcca gtaaacctgt
     5821 aaaaactgcc gaagtgagat gacgtctaag gatggactca atggacttat aagcaaaaaa
     5881 aacaatacct agtaaaggcc atattttacc atccaaagaa ggaattccaa aattcccttg
     5941 cagcaagatc caagctgcta aaaccaataa aacaatacca aatgctttct ttttcatgtt
     6001 ttttacctca tgtttcttag attttctttt aggagggatt ggtaatgccg tgagacatga
     6061 acctccttgt gcgtctgata aaaggaaatg gtgcccgttc ctgaaaagga cttgtccact
     6121 gagtaaatct gacggatgtt tgcgatcgtt gacttggaaa ctcgattaaa atagcgaggc
     6181 aagatagact ccaactcata gagcttgagg cgaacctcat aagcttcctt ctggttatga
     6241 gcgtagatct tgctcccttc tgtttcaaag aagagaattt ctgacaggtc tagataatat
     6301 tcacctgtcc ccttgtaaaa agtcaacctc ggagcctttg actcttgcaa gagccgttgc
     6361 aagtctgcaa tttcatctgt caaagtgggg gtcttgatga caatttcagt ctcctctaaa
     6421 ttgccgtcaa tctcaattcg taacttcatc acatctcctt tctgactcta ctatatcaaa
     6481 gctaagttag gaatacaagg gcaaaaaagc aagtggtcaa tttgaagcag taagtggttg
     6541 taggactagc ttaacttaca tgtcagaata ggaagaaaag gaatccccac accaaaccac
     6601 aagcatagcc aaccaagaca tccttgggat aatgaacacc ccctaaaacc ctcaccaacc
     6661 ccagcaaagc tgaaaagatc aagcatacta agccaatagg tagactagca tgcagacagg
     6721 ccacggagat gatagttgcc gaaaagacat ggcgactggg catcgaccgt cccgaactat
     6781 ccttatcaag aaggggacag atatcccaag tttcataagg tctcgggtga ttgattttct
     6841 tacgaaacag ggacaaggcc acaaaacctg atgcaggaat aagcacataa accccaactt
     6901 gctgaccaag tcctagttgc aggtaagtgg tgactagcaa gatcagatag accaggggca
     6961 tggcgactgt catcaaacga ttgaaactgc gtaataggaa taaaagaatt ggacgactag
     7021 ttagtctgga gctgatattt cgataccatt cttgataatt tttcatttat gcttctcata
     7081 cttttctaga acttgttgag atgttttccc cttggcaagc caatccacta gcttgtccaa
     7141 gcgacgtatt tcctgcatca gaggatcatc aatttcctca atacggacac cgcaaatctt
     7201 ccctgtaatt gcaattcgtt cagggacata atatggtgct tgtctaaaaa aatctccgta
     7261 agtaacatcc ctatccagac aagcaagaac atcactcact tcataccctg tcaaccaact
     7321 ggtcacttga tgaactgact ctgcctttcc accctttcgc tcaacttttg caatcaaggc
     7381 taagtagaca gcagcaaatt tcatattgta taatttctga ctcatacttc tctctttcta
     7441 tgtgtactag tataaataag aaaaagaagg ccgtcaagcc ttcttttgat ttattcttct
     7501 gcttcatctt ctgtaaattg actattgtac aagtcagcgt agaagccacc ttgcgccatc
     7561 agttcctcat agttgccttg ctcgatgata tttccatctt tcatgaccaa gatcaagtct
     7621 gcatttcgga tggttgacaa gcggtgggca atgacaaaga atgtgcgtcc ttccatcaaa
     7681 cggtccatgg ctttttggat caattcctct gtccgtgtgt caacagaaga agtcgcctca
     7741 tccaaaatca aaagcggtgc atccttaaga agggcacgag caatagtcaa tagttgtttt
     7801 tgtcctacag acaaggtcac ggtgtcatcc aagatggtat catagccatc tggcaaggtc
     7861 ataataaagt ggtgaattcc cacagcctta ctagcttcca tcattcgttc atcactaatc
     7921 cctatttgat tatagatgag attgtctcga atagttcctt caaagagcca ggtatcctgc
     7981 aagaccattg aaaaggcatc atgcacttct gaacgcgtca tagccttggt atccacacca
     8041 tcaatgtgaa tacttccctt atcaatctca tagaatttca tcaaaagatt gacaatggtt
     8101 gtcttaccag tcccagtcgg cccaacaatg gcaacctttt gaccagcatg agctgtcgca
     8161 gagaagtcat agtcttgaac attgacaccg tccaccagaa tttctcctgc tgacacgtcg
     8221 tagaaacgtg gaatcagatt gaccagagtt gatttaccag aacctgttga cccaataaag
     8281 gccactgttt gaccagtttc tgctttaaag ctaacatgtt caataactgc ctccgaattt
     8341 gccgcatagc ggaaggtcac atccttaaac tcgacctgac ctttgaagtt ttcatcagtc
     8401 agctgcactt gaacagggtt ttggatagaa gaatgcaaat ataaaacttg attaatccgc
     8461 ttagcagaga ccatagttcg gggaagaacg atgaagagtg ctcccatgag aaggaagccc
     8521 atgacaacct acatggcata agacatgaaa acaatcatgt cactaaagag aggcagacgc
     8581 gctatcggag cagcgtcgtt aatcacatag gccccaatcc agtaaatcgc cacactcaaa
     8641 ccacttgaaa tccccatcat gataggattc aaaatagcca taagacggtt gacaaacaaa
     8701 ttcaaacggg tcaattcatc atttactgct gcaaattttt cattttgata atcctctgca
     8761 ttgtaggcac gaacgacacg aatacctgtt aaactctcac gagtgatact gttcagttta
     8821 tctgtcagcc cctgaatcaa ggactgtttt ggaaaggcta gcgtcatcaa aacggtcgtc
     8881 atcaggacgt tgacaatcac tgccacaagt acggcccaga gccagtattc tgaatgacct
     8941 aaaatcttcc caatagccca gatagccata attggaccac acgttaccac ttgcaagccc
     9001 atagtaatca acatttgaac ttgagtaatg tcattggtag tacgcgtcaa gaggctagga
     9061 attgaaaatt tcttaatctc tgtctgcgag taatccaaaa ctcggttaaa aatatcactt
     9121 ctcagcctac tagtataaga agccgccact cgggatgcaa aaaatccaac tgcaactacg
     9181 gacaagaagg caagaaagag cattcccatc atcatgcttg ccggctgcca caactcatct
     9241 aaattagttc cttgactacc tagcaaatcc gtaattttcg agatataggt cggcacttcc
     9301 aactctagat agaccgaaaa gcaagtaaag agaatggcta gtaaaatcat ccccattctt
     9361 ttctactaat tcttttggct aatttcttta ttctctcctc ctattccctt gatatttgcc
     9421 tgtagttgac cgagaacttt ctcaaaaatc agtaattcat cttcatcaat gtcttccatc
     9481 aactgcttgt ctatgcgttc aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg
     9541 tccgtcagac gaacaaactt agcccgctta tcaacaggac tcgcctccaa ttccaccaaa
     9601 ccattttgca ctatacgctt aaccagatta ctagcaacag acttggtaat attgagttcc
     9661 tgctcgatat ctttaatcaa gaccaagtct tggtttttct cgcgattatc caaaaaacgc
     9721 acaacctgac cttgcggccc acccataaat tcaatgccgc aacgtttggc ttccttttgc
     9781 accatcaggt gaatttgatg accaaaacgc ttaaagacta acatcggttt acccataatc
     9841 tcccccttct aaataaaaat agttctctgg agaataatta aatttctatg agaactattt
     9901 tcttgattaa aaaaatccca agtgattttc tcacttagga tcatgttcta taggttaaat
     9961 taaaacccat ctacgttcgt ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg
    10021 taaagttttt caaaggtttc aaggtcttcg cctgacaatt ccacttctga ctgaggaatc
    10081 atttccaatt cagtcacttg gaattcttca ataccagact cacggagggc aacgatagcc
    10141 ttgtgaaggt cagttggcgc tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca
    10201 tccacatcca catccgcttc gagcaattgc tcaaagactg cgtccgcatc ttcacctcca
    10261 aatacaataa cacctttgtt gtcaaagagg taagaaacag aacctgaagc gcccatgttt
    10321 ccgccgtttt taccaaaggc tgcacggaca ttggctgctg tacggttgac gttagaagtc
    10381 aaagtatcca caattagcat agagccattt ggcccaaaac cttcgtaacg tccttctgta
    10441 aaggtttcgt ctgtgtttcc tttggcttta tcaatcgctt tatcgataat gtgttttggc
    10501 acttgggctt gtttagcacg gtcgataacg aatttcaaag ctgagtttga ttctggatct
    10561 ggatcacctt ttttagctgc tacatagatt tctacaccaa attttgcata tactttagag
    10621 ttggctccat ctttagccgt tttcttggct acgatattgg cccatttacg tcccattagg
    10681 aatctccttt tttcacattt taatctttct tattataaca caagtttttt tgattttcac
    10741 tagaggaaat ggattttatt agcaaatcaa gctaggatag cactttacct gctaagatgg
    10801 tcttgccttt ctatcttttt atcaacagac actcatccac attcaaaaaa caaactagac
    10861 cattatctgc aaatagaaag tttcagccaa gtttgacaaa gtcagctcaa attactgttt
    10921 gaagtttgta gatataagcg acaaaaacaa tcatactgca ccttttgttg acagtctact
    10981 ccagacatat catagttcaa gtaaatactt tgaaatccaa cagttcttat aggcgctatt
    11041 gtattctaag aaatcaatag aagagtttct aagcaaatct ctaatactca ataaaaatca
    11101 aagagcaaac tagaaagcta gcctcaggtt gctcaaaaca ctgttttgag gttgcggata
    11161 gggctgacat ggtttgaaga gattttcgaa gagtataatt tacgtgttcc caagatggag
    11221 aagttagact agtacactgg cacttctaaa ccattgctag caattgattt gttcatattt
    11281 aatttcattt tttccataaa tgggtattag atataaacag caaaatattt ccgatacgtg
    11341 tcgttcttga atttccaatc atctaaaaca agtaaaggat aatcaatccc ctgtatatca
    11401 aggaattggc tacccttttt acttttttac acattctgtt tgatagattc attttaacat
    11461 cacgagcata ctccaatgga aatcgctagg caagagataa actttcagat atccgcagag
    11521 agatcatcgc ctctttttgt cgcaagcatt ctcctctcct agtcattttc taccttatct
    11581 tctacctgcg gatagagagt tgttccccaa atagaaatcg tccgcttacg cactagtggc
    11641 aaatcggttt tttcataaac cgtacgccac cattcccaag caagcccggt acactctcta
    11701 attttgacag agagattacg aacattccct tttaaaggaa tactagtggt aaagtgagcc
    11761 gttaaatcct gcccatttct gtcccaagcc ttaggagtca agacttcctt accttgatga
    11821 tcataggata attcattcca agtaatataa tattgggcaa cataggcacc actatgatcc
    11881 agcagtaaat ctccgtttct gtaagctgta accttagtct caacatagtc tgtactattt
    11941 tgaaaggtcg caactacatt gtcacgtaaa aaagaagttg tataggaaat cggcaagcct
    12001 ggatgatctg ctgtaaagcg actgccttct tgaatcaagt cctctaccat atccaccttg
    12061 cctgttacaa ctcgggcacc cgaacttggg tcgcccccta aaataaccgc cttcacttct
    12121 gtattgtcca aaatctgctt ccactctgtc tgaggagcta ccttgactcc ttttatcaaa
    12181 gcttcaaaag cagcctctac ttcatcactc ttactcgtgg tttccaactt gagatagact
    12241 tggcgcccat aagcaacact cgaaatatag accaaaggac gctctgcaga aattcctctc
    12301 tgttttaaat cctctaccgt tacagtatct tgaaacacat ctcctggatt tttaacagcg
    12361 tctacgctga ctgtataata aatctgctta aaattaacaa tctgaatctg cttttcgcct
    12421 gaatggacag agttaaaatc aatatcaaga gaattccctg tcttttcaaa gtcagaacca
    12481 aacttgacct tgagttgttc catgctgtga gccgtgattt tttcatactg cattctagct
    12541 gggacattat tgacctgacc ataatcttga tgccacttag ccaacaaatc gtttaccgct
    12601 ccgcgaacac ttgaattgct ggggtcttcc acttggagaa agctatcgct acttgccaaa
    12661 ccaggcaaat caatactata agtcatcgga gcacgatcga ccgcaagaag agtgggatta
    12721 ttctctaaca aggtctcatc cactacgaga agtgctccag gatagaggcg actgtcgttg
    12781 gtagctgtta cagaaatatc acttgtattt gtcgacaagc tccgcttctt tctttcgata
    12841 acaacaaact catcgggtag ctgattaccc tctttgatga aacgattttc aatactttct
    12901 ccctgatggg tcaagagttt ctttttatcg taattcatag ctagtataaa gtcatttact
    12961 gctttatttg ccatcttcta cctcctaata agttcctgga ttgagttgca taaactcaga
    13021 cttgttcagc gaaatcagcc gtggttggac taagtaatcc aaaatttcct cgtacaattc
    13081 ttctgagaca ttgcgtcgcc gtctggctag ataagaagtc ggaataaccg tattatccaa
    13141 cataaatacc ttatctaagt caatcaaggt tggtcttgta aaaggattac gagctagatc
    13201 cggctcttct atcataaagt tcttgaccaa acgtctggtc aagagagctg gtttgaaggt
    13261 ctgattttta accaactctt tgtttttagt catgctgttg tcaatacaga tatacatatg
    13321 attcttcaca gccaaatcgc tactaatagt cggaaaaggc aaataaagag ctacaacatc
    13381 tcctctctta atcaagcaag agcaccccct tttctcctaa tgtaacatag acaggattga
    13441 ccaagtcttc tgattgactc agaatttcca aagtttgagt ttggcgcgct gtcaatttag
    13501 tagcatcttg tctcttcaat acaaaatgct tgtcgccaat aaccttgaca atataatcct
    13561 tctccaaagc tgactggtaa atccacatca gatgttgtct gtcctgagaa ctcaagagag
    13621 aaggattttc aagcctcccg atagtctgat aaaaatcaaa aacaggagct aactcctgcc
    13681 aatctgattg gctagttgtc aaggctagaa aaagggcttt gcgagctgat acttcttggt
    13741 tagccttgag agttactttc ccctccaagt tttttagaaa tcgggaaact ccagaaagca
    13801 aatttttctc taactgcgag aaataaaaac ctttcgttcc cagacataag tctttcatgt
    13861 cgctttctct agcaaataag agctcaaaca tttgatagta aaagaaaaat atctggcact
    13921 gggtcgcgct catcttttcc ttatcggctt ctttttttaa ccagagcaag ggcgacaggt
    13981 agctggattg agacatttcc tctacctcct actctttttt aactggagca tctgcactag
    14041 ctgccacttc ttttgactgg atactttccc actggttaat ctcctctgag ataagacctt
    14101 cgcatgtctt gacaaatagg gcaaaagcct tggtctttcc tgcatatttc tccgtttggc
    14161 attgatagag gaatttttct ttctccagga gttgcgcagt tttttggtaa gaaatccaat
    14221 tttcctttgc attatacaaa ttgataatcc cctcacacag caagccgaga ctggataagg
    14281 caaccgaaat caaacggtag cgatcacctg gcataggaat agcacaaaag acagctatga
    14341 ggaaacctgc cacgatttct gttattttta ataccttata gcgcctacga tgttgaacgc
    14401 ttttctttaa aaaatgagct atctgtacgt ctaatcgctc tgtcaggtac atttcttctg
    14461 gcgtcatatt cgtaactcct ttcatttact ttgataatca tagtataaca tatttgaata
    14521 tcaaagtata gtaaaaactg gactataact tattagactt cctacgaaac aaaatatggt
    14581 atagtagttc tatgaatgat gaagcaagta aacaactaac tgatgcacga tttaagctcc
    14641 ttgttggtgt tcagcgtacc acttttgaag agatgttagc tgtattaaaa acagcttatc
    14701 aaaaaagtcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gcccatgaag
    14761 atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagtcgct aatagctgaa
    14821 gataaagcct ataaccatgc gctatctaag gagagaagca aggttgagaa catctttgcc
    14881 aaagtagaaa cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga
    14941 ttacgaatga atttgattgc tggtattatc aatcatgaac taggattttg gtttcgcagg
    15001 aagtctatta tatatgcaca gacatatgaa atctttttga gatagcagtt atgatatatt
    15061 tatccattca caacatcttc tgtactaata ctacaatatt ggtacatttt gattacacaa
    15121 tcgtttcaaa aaatatgatg gaagcctgag tattttatag taattccctt cctatcaact
    15181 agatcaacca gttgagtgtt gaatgttgaa acaccctagc ctcagcaaag gactatgatc
    15241 agcatttcct agccaatagg acgctccaaa tcacctattc taagtccatc actagcttgg
    15301 tcagtaagat acagactgca aggcgagact gacgtggttg atgagatttt ggaagagtat
    15361 tacaatcata gattcaccag tagacttgac cgcagattta actatagtat attgaaacta
    15421 gaatagtaca cctctacttc taaaacattg ttagaaatcg atttgactgt cctgatcaat
    15481 ttgtcctgtt cttttttcat ttcactatac taaaaaacca cctgaaacaa atcaagtgac
    15541 tagaggataa aaacaaagac tgggcaaaaa gttttgaaaa gcaaaaaacg cataatatca
    15601 ggtcttgaaa aaccttgacg tttgattgtg ggaagattga cttcattttc tcctgaaatt
    15661 gaagtttttt ctagtttcca gatatagacg aattcaatat agccatctcg ctacaattat
    15721 tttaaccatc gagaaaattt ttcagaattt caaaaatttc attttgatcc aatgaaatgt
    15781 catcattttg aagaatagtt gacttcacag gctctaattc tatcacaaag cggtgtatgt
    15841 ctgcttttac cgcactactt gatgttatag ccgataactt ttcatctcct agcaaggaag
    15901 ctgtcaaacg ggtaatgtca ttcaaatgct ttttaatgga cttacttaaa ccttgagcac
    15961 ctgtagcaga gcgttccctc atatccagcc aagctttagc cttaaaaata attaatactc
    16021 aatgaaaatc aaagagcaaa ctaggaagct agctgcaggc tgctcaaaac actgttttga
    16081 ggttgtagat ggaagctgac gtggtttgaa gagattttcg aagagtataa accacaatta
    16141 ctcaataccg aatacccctg aatggtttct ttttcatgca ccaatatatt ataataatct
    16201 tcatccaata ataaggctga taaactagca tcttggtcaa aatgtaaggg aatttctcga
    16261 ccgtccttct ttaatggata ttctggtaag atactaaata gttcaatcat agaaggaaac
    16321 tcaggattag ttgttgtaaa tcgaaaaagc tgcgctttct catctttctg acttccttga
    16381 tactctccca attctaaaaa atgattcaag gtagtataaa attccttatt ttttacttca
    16441 taaatgatga ccatatcata atcttttgtt gtgcgacttt taaatccttg cgaatccaat
    16501 acgatagagg tagcagttcc cccaatcaga acataatagt tctgaaaatc cgcaaacatt
    16561 tcttgaaaaa taactttcgt attagctggc atcatcttct cccagatact gtaatatcat
    16621 attttctagt gcttcactct cttcctctat acgtgggtca tcatcatctt ttaaggtcaa
    16681 ataaagagaa atcggatcta caaattgttt atcatgatta tttttaaaat cattccaaaa
    16741 ctcagataca aaaggacgat atttccatat ctctagcatc tttcctttta aaacatgctg
    16801 agaaagtggc aaggataact gattgaattt tctctgccat atgacatagc tagtattttc
    16861 atccgtttca gctaaaaaag ttgaatgcga caaagcataa gcaccaccat atagaaggtt
    16921 agaaacagat tttatctgct ttatatcgcc atctggcaat aaaatccgtt ttttgatggg
    16981 attaaataaa catgacacgg attttaagaa taattctttc tttgacaccg tatatgtgta
    17041 aagcttattt tgcttgttta accaatataa agctttaaaa gtcctcaaac acctataaat
    17101 tgttgagttt ggaagtccag tgacttgtga aagcaaatca acatctacta ctttttgacc
    17161 ttttgtcaat aaaaaggcaa tccacgttaa ttgttcgcta ggtgttaatt ccttagggac
    17221 ttcagtatca ttcgcattca gtactagtcc caatggaggg aagaagaggt ttcccttaaa
    17281 gtctacaaac ggaactctag cttgaagtaa ttgctttttt tcactgtctg ataacttcga
    17341 aaacaccaaa acaacatcca tattggcttt ttcacccata gtgcgagcct gagtaacaaa
    17401 tgaactcaaa ctcccccttc tcttttcttt aatcaataaa aatgattcct tatgaaaagt
    17461 catttgagta tactcaaacc tttttacaaa actgattggt aaagtcaaag tcagttcctc
    17521 aatactgaca tcaacataaa aggattgaaa aattgacatt atctttttga cattcatgtt
    17581 ttttccttat tctatcattt ttattatatt ttatcattcg gtataataaa atataagttt
    17641 tttgataaaa taaccaaaaa ctactcgcta tcccctctca cattacccta catatcgttt
    17701 gacatgcgac tgatagttca ggaaaacttc aaggagaact tttctctcat ccactatgca
    17761 ggacttacta tttcacttct actcctatag gctcaatttg acacttttct ttttcgaatt
    17821 gctcatatgc ctcctgaata gtgagttcga tttatccaaa tttatttttg tctattttga
    17881 tgaatacttc cacgaagata gtctgaaaat gaatttgcac caattttcct tctcttttta
    17941 agcatattat tccagatatg attgccctct tgttcagtaa taaattacgc tttaaacgct
    18001 tcaatcagta tatctcctgt tgtcatatgt tctaaagaaa attcttctac atatggttta
    18061 acatctctta ggttattact tcctaatatc ccattatgct ttttcgctaa ggaaatagaa
    18121 gccacttctc ccttaccaat aatcttgtta ctatcatgat ttcttgttaa atctctatat
    18181 aatgcgtatt cttcagttcc aatgtctatg ctcacaatct cagctgaacc cttagttacc
    18241 aactggtcta tcctagattt taaatgggga attgtaggta tattgatttc atcatacacc
    18301 tcttgtggaa taacaatttt acccgaatag agcttttcta aaagatgttc agtaccaacc
    18361 cataaaaata ctgaaataca atctgcatca ataaatacac gactagtcaa acgacaactc
    18421 ccccctcttc atctagccca tatacaatat catatctgaa agcatctagt aacagttcct
    18481 catacttccc ttgcgaaatt ctgttatttt ctaaaagttg ttcagttgaa ttaacatatt
    18541 atcctaatat catttacttt ttactttctg acaaaggacg atataaactt gtatcatagc
    18601 ctaatcttga agctgtctct ataacactaa tatccatatt tttaatttct tctgcatcaa
    18661 ggtatccatc attcctcaat ctatataaca taactttatg actgatacca taaaactgac
    18721 ccaattttat aatatcttct acttcaagat gagttctatt ggcattttct ctaatttcct
    18781 caaccatcct atacagtgaa gatgggaaaa ttaaaaaata agaagcaaac tgatccgctt
    18841 ttctttcagt ttcatctcct tcaccaatca agataagact gactgaactc ttcttcacct
    18901 catcataata aagatgatac agttcatgtg ctaaagaaaa tctttacctt cctaatgaca
    18961 tgtctgaatt gactgcaatg agactgaact gagttccttt ataacagact ccgctaatat
    19021 tctttccgag tccataaaat accagcgtca aattttctat cttttgtacc aatttaaaaa
    19081 tatctatcgg cgattcacca tcagctccca acttttttct aagatttgaa gctttattac
    19141 ttaaatccat tcgtgatatt cctttcacct ttttccattt tggcaatcat gctttgattc
    19201 aaagacaaat actcagcaat cttatcttga gtaagatttg aagatattct aagttctttt
    19261 attctttttc caacatcaca tacatttatc acatacattt atcatattca tacacctctc
    19321 ataaaaagaa tagcacactc ttgtcataat tttttaaata aaaaaattat gacaaaacaa
    19381 ggaagcaatt tattgatgct gcttaaaaat ctaaaattga tgagttccta tcaaaaatcg
    19441 tatcttcaac ctcaaaacag tacttaaagc tatccgactc ggtttacatt gtcaaattta
    19501 gattttattt gagcataact ttctagtttg ctttttgatt tttgtttaat atagtagcaa
    19561 aattgaagga aatatctcca caagaaagcg catactatta agctttttca agacctaata
    19621 atatgcgctg ttctgatttg aaagacattc cattattatt ttactgtaat caagccatct
    19681 ggctctactg tgaattctgg cttgtctgcc agtgttccgt ctggtttgag gtagtaccag
    19741 cctgttccgt ccgctgactg gataaaggca tttgatacca tggcgccttc tttagcgtct
    19801 aagtagtacc aagtgtcctt gtacttgacc cagcctgtct tcatggcacc ttcttcgttg
    19861 aaatagtacc acttatcagc gattttcttc cagcctgtag ccatttcgcc tgagttgtcg
    19921 aaccagtacc agttgccgtc tgtgtgcttc ctccagcggt ctgcaagcat atagcctgaa
    19981 ctgtcaaagt agtaccaagt gccattgatt ttctcaaact tgtcttttgg ataagagccg
    20041 tctgaatgta cgtaccagta gccagtgtca ttcttctgcc agcctgtttc aatcgtcaag
    20101 ccgttctcaa tatcatgctt aaactgctca cggctaatgc cccatttagc aagatatgga
    20161 taagggtcaa cgtggtctga gtggttgttt ggttggttat tcgtgcaata ctcgtgcgtt
    20221 ttaattccag ctaaactccc tgtatcaagc gttttcggca aacctgcttc atctgctaga
    20281 ttgcgtaaga gttcgatata aaggcggtag tccgtcatga actcttcttt ggttgaatgg
    20341 ctttcaatca gttcaaccgc tgcataggtc tcagcattcc aaccgccccc aacgtcccag
    20401 gcaccattat caacaggtcc tacctgcatg atgcaaccgt tcccaacaat gtgcgagaaa
    20461 aaacctaatt ctgggtcttt ccgccagtga taatccgctt cattctgtac ggttgaatgc
    20521 ggattcccag ttgagtgtgc gtgtacttgc ctatatggtt gcacgccgac ttgaggcaaa
    20581 tctgttctta atttactcac attaatttcc atattctact ccttatcaat taaaacaact
    20641 cattttttac aatccagaac cagaaactcc tttatttcta ccttacaaag aagacaatct
    20701 tagtcacgat taggcttgta gatagaactt caaaacgcac tattttgaca ctgtaaatag
    20761 gactgacaag gtctgcattc tatctacaat aacaccccag actaaaaagc ttttcaaaag
    20821 tatattttta cagtctctat atgtcctttt cataaatact atactttatt atatcatata
    20881 aaagaagtca aaagtctgtt aaactatttt caacaccaaa ctaaagaaga gaacacaagt
    20941 tttttcgatt ttcactagag gaaatggatt ttattcagta aatccaacta ggattgcact
    21001 ttggttgcca aaattgcctt tccttctttt atcaagggat gacggaatag tgagaagtac
    21061 agttgaactg tcatggcaac ccagataaga ggttcacaaa ggataacacc cttatatcct
    21121 gcccaaggaa taatcaaaac cacaaaaacg atttttccga ttagttcaat aaagctagaa
    21181 actagaggaa ggatcttctg ccccaagccc tgcaagcaat tgcgataaat caacaagagg
    21241 ctcaaaatgg gataaaaggc tgaactgatt tgcagataga gacttccatt ttctatcaag
    21301 taaccatctg tcgaactagc caagaaggaa accaaggctg gactggcaaa aaagaggaaa
    21361 atacaaacaa aaactgccca ggatatactt aaacgactgc cgattcgaag accttgaaca
    21421 atgcggtcag gtcgcttagc tcctagattc tgagaagcaa aggtcgtcat tgatgcagaa
    21481 atagcggtca taggaagaag ggcaaaggtc ataatgcgtc gagctgccgt ctgggcacta
    21541 ataatcactg caccaaatgt attaacagaa gactgtaaaa tcacactgcc gatagataca
    21601 attgaactca tcaagcccat agccaaaccc tgctccaaga gatccgcgta caagcttttg
    21661 tcccatttga aatgtttaaa ctgtggcaag agttctggca cacttttacg aatataataa
    21721 aagcagagaa ccgctgataa accttgcgaa ataatggtag caagtcctgc ggattgaact
    21781 cccagatgca attgcgtaat aaaatagaga tccagaacca cattaaccaa ggcagagaaa
    21841 atcagaaatc ccagggctgc tagactgtca ccaatagacc gcaacaagcc tgcaaaaaga
    21901 ttataagcaa agctgacacc tacacaggtc acaatcatag aaatatattg ataagattga
    21961 ggaagaattt ctgcaggagt atctaagtat tgcaagagag gatacaagcc aagaaagccc
    22021 agcaacataa ctagaatgct caaaagagca cctaaaatcc aggtggctgc tactgcttcc
    22081 ttgattttag tgaaattccg agccccataa taacgagcaa tgacaatccc catgccattg
    22141 ccaacaccaa gtgtaaaacc tacaatcagg tcaaaaatcg ctgtcgtcgc tcctactgca
    22201 gccaaggatt cttgaccaag aaatcgtcca acaatcaaga catcagcagt gttatagagc
    22261 tgttgaaaaa tatttgatag caagattggg aaggcgaagc ttaagagcga gagaagaatc
    22321 ggaccatgta tcaggtccac tgttcgtttt ttattcataa agctattata tcagctttcc
    22381 agaggagcga caagaaacag caaacttata tatagcggaa tgaaataagg acagtcaaat
    22441 cgatttttaa caatgtttta gaagtagatg tgtactattc tagtttcgat ctattatagt
    22501 tggttgagtt tggaatagta cgctgtagct gctaaaacat ttctagaaat taatttgact
    22561 ttcctaataa agttgttcat atcttatttc aagctactat atataatcta aaaacttgaa
    22621 tcaaaaatcg aaaaagtagc ggatttctcc actgctttaa cagcttattc ttcaatttcg
    22681 atttcaagtt catcgcctaa gtcaagcgta acttcttcat tcactgcttc ttctttcttt
    22741 ggctcatctt ttttgttttc agtatcttgt tctgaaactt cttctccatc aatcaagcca
    22801 aatttagaac ggacttgctt atcaatttca tcaaagattt ctgggtgctc tgccaagtat
    22861 ttcttagcat tctcagaacc ttgcccaatt ttttcatctt tgtaagaata ccaagcccct
    22921 gcttttttga taatatccaa atcgcttgca atcttcaaaa gctcaccagt cttagaaatt
    22981 ccttctccgt acataatttc aactacggct tccttaaacg gtggagctac cttatttttt
    23041 acaaccttaa tcttagtttc tttaccgaca ttggtttctt tttgatcacc agttccctta
    23101 atttgtgtat taccacgaac atccaagcgg actgaagcat agaatttcaa agcacgtccg
    23161 cccggtgttg tttctggatt tccaaacatc actccaactt tttcacgcaa ttggttgata
    23221 aaaatggcaa ttgttttggt tttattgata gaggcgccaa gtttacgcat ggcctggctc
    23281 atcatacgag cctgcaaacc aacatggcta tctccgatat ctccatcaat ttccgcacga
    23341 ggaacaaggg cagcaactga gtcgactacg acaagatcaa ctgcacctga gtcaatcaat
    23401 tttcccgcaa tttcaagacc ttgctctcct gagtctggtt gagacaagag caattcgtca
    23461 atattgacac caagggccgc agcataagct ggatcaaggg catgttccgc atcgataaag
    23521 gcagcaatcc caccttcttt ttgcgcttgt gcaactgcat gaagggcaac cgttgtctta
    23581 ccagatgact ctgggccata gatttcgatg atacgtccct taggataacc acctgagcca
    23641 agggcaatgt caagagctaa agaacctgag ctcatcactt gcaccttttg ctccgcacgt
    23701 tcacccaaac gcatgattga tcctttacca aagtctttct caatcaattt aagagcgtca
    23761 ttcaaggcct tttcacgttc tgccccaaat ttttttgaaa tttcttctaa tttttttggt
    23821 tttttcgcca ttctattctc ctacattcta atgatcctca gacctatcta ctattatatc
    23881 aaaagttagt cacttaataa agccttgcga actaggttaa aggcatgcat aaccgcaatg
    23941 tgacgtacat ctgctcggct tctgcctcca atattaacct tgataacctc agttccttga
    24001 tcttgcgcca agcctatgaa gactgtccca actgggtgtc cttctaggct atctggtcct
    24061 gccactccag tcaaactaat tccaaaatca gactgggtct tgcttcgtgc ctgctcagcc
    24121 atcttctgag ctgtaaattc agacaccaca ccatgttctt ccaaattctt ggcaggaata
    24181 tccaacatcc ttgatttttc ctccaagcta taggtcacaa aaccaccctc aaatatactt
    24241 gaaactccag aaaaattcgc cacggtagct tggaaaagac ctgccgtcaa actctctgca
    24301 gccgcgatgg ttttcccttg ccttttcagt tcttctacca caatgctggc taaactagtt
    24361 tcttccccat aaccatagca aaagtctcgt aaagaaattc cttcgaaagt ctggcagtcc
    24421 aagatttgat tttccaagat atccagcgct tgattcgcct cttcttgact gctagccttt
    24481 gttgacagac gtagagtgac ttctcctgtc ttggcataag gggccaaggt aggatcgatc
    24541 tgattatcaa ttaaatcagc caaaatcgta accaactggc tctcgccaat cccaaagaaa
    24601 cgaagaactc gggaatacag cttgctccct gtcatcaact tgggtagaag ttggtttaag
    24661 accatgggtt tcaattcact tggcggacct ggaaggacga cataggtcac tccgtctact
    24721 tctaattttc ctccaacagc cagtcctgtt tcgtttggca gtggaatcgc tccttctaca
    24781 atttgagctt gtctttcgtt atttggtgtt cgggcatagt ctggtcgcag ggtaaaaaag
    24841 atatccaact tctcctgagc ctgaggatca aagactaatg ctttccctaa aaatttagct
    24901 agggtttgtt tggttaggtc gtcctcagtt ggccccaaac cgcctgtcaa aatcaccaga
    24961 ctgctacgtt gactggcaat ctcaagcaaa gacaagagac gaacttcatt gtctcctaca
    25021 gccgtctgaa aatatacatc taccccaatc tcagctagtt tttccgacaa aaactgggca
    25081 ttggtgttga caatctgtcc tgtcaaaatc tctgttccaa cagcaatgat ttctgctttc
    25141 atgtttcctc ctacctatct attcgtattt ttttgaaaaa atcgcaggaa ttttcctacg
    25201 attgattttt tatttgtatc aaaagttaat tatcttcatc accaacaggt gctctgccaa
    25261 ataaatcttc aaataaaacc gcattggttt caagctgagt aacttcttct tgtcccaaag
    25321 aacgtcggag tatattttgc atttccaaca tatgtgctct cgaaacaatc tggtaagaaa
    25381 caccttgaag tatctctcct tcaccctgca actgctgagt ttcaatggtt ttaaatgaat
    25441 ctttatagcc tagcaagtta gggatacttt ttgcagacaa atcaatattg gtctgcatat
    25501 tgtcactcaa agcttttaga atctcttgat aatgaccaac gctatttaaa ctgagagctt
    25561 tttccatgac tttttgaata acttcacgtt gacgtttttg acgaccataa tccccctcag
    25621 gatcttggta acgcattcgt gcatagacta gggcttcttc tcccccaata tgttgctccc
    25681 caacaccgat agaaatagta ttaaattctt cttggtcact gatagaaatt gggaaaccta
    25741 ggatattatt gactgtaata cctcctactg catccactag tttttgcaac cctctcatat
    25801 tgaccatcac atagcgatca atatggatat tcatcatttt ttgaatggtt tctatagcaa
    25861 gctctgctcc accatctgca tatgctgagt tcagtttcgc ttcatgagcc tgaccattcc
    25921 ctgattcaat gcgcgtcaga atatcccgct ctaaactcat cattgttgtt tttttcgttt
    25981 taggattcac tgtcatcaag atcatgctat cacttctacc gacccaagtt tcagttcgtt
    26041 cagcatttcc ggtgtccact cccattaaca gaatggttag aggttcagtc gcttcaataa
    26101 ccttggtttc ttcgccgatt tttttatagg ttttagctaa ggtttctgtc ccttgttgat
    26161 aaatagtata agcaaaaaca cctactccta ctacagttac agaaagtaaa gctagcacca
    26221 ttccaataat ttttttaacc atatttctac taacctatca gtttacccat caagtaaaca
    26281 tcgataaatt tcccttcttc tatatatgcc ccacgctctt ggctaccttc aatgacaaag
    26341 ccatgctttt gataaagatg gactgctgct tgattacgag tttggacagt cagttggaga
    26401 cgacgcagaa tgccacttgc ttgtgcccac tctgtcgctt cttctagcaa caaacttccc
    26461 aagccattat tccaatatct ttttccaatc acaatgaaga gatctccaat atgacggact
    26521 ctcttacgct gatcagctgt aatatttaca ataccagcaa ttttgccatt taagaatgca
    26581 agtaaggtta tctgattgtc cgaactagct tgcttgttga ggaatatttc catctcctca
    26641 ctagtcaaga gaataccatc tccgtctagg ctggtaaagt ctgtctccaa actcacacga
    26701 tttaaaaagg ccactaattc agctgcatct ttgggctctg cttccctaat gagcaattca
    26761 tactccatat tgaagctcct ctaacaattt ctcagcacgc aaaccctttg cctgaaaatt
    26821 taaacggcgt ccatctgctt cttttagaat ttccaattct aaataagcat ctggcaaggc
    26881 atctcctaag agatttcccc actcaataac agtcacgccg ccaccaaaga taaactcatc
    26941 caagtcgata gaatcagcat ctccttcaat acgataaaca tctaggtgat aaagtggaag
    27001 tcgaccttca tactctctca cgatagtata ggtgggactt ttaatcattt gagaaatctg
    27061 taatcctttt gcaagtcctt tagtaaaggt cgttttacct gcacccagtt ctccagttaa
    27121 gattacaaca tcattctttg ctaatagatg gcccaaacgc tcccctaagg cttgcaactc
    27181 ttcttcattt tttgtgtaca tactcttatt ataccaaaaa cttttctttt gtgtctattt
    27241 tcctactaaa cttatcatca taacatccat aaaaaacaga ctttctctaa aagaaaatga
    27301 gcgtaacaat gaccaataca agatctcgga aaatatgacc ataaaaggaa acttccttct
    27361 taaccgaatt tgggacaaga taggctgcaa aaaacaagcc cagtccaata taaatcagaa
    27421 gtgagacaat ggtcattgga tttcttaaga aaagaagtgt tgctaaaata gtcaccaaca
    27481 ctgtcttttt tctgtctagc atagcaagaa aatcgcgcac gtattttttc aagggtaaaa
    27541 aaatcagcaa atctagccca aataggaaaa agaaggatgg caataaaaag tcaactaatt
    27601 cttgctgcag cgtatttttg atgaacaagt tatctgacaa aacaagaaca gctcctaaca
    27661 aattaattaa gagtaacata ctgtaaaaaa gcttcaccga cttcttactg gctaggacac
    27721 tatggacttc ttgcttacgg gtataaagat aatttactcc agcacagatt cctgaaacga
    27781 aaaccatgct tccgatgaaa aaagctgtac tttgtttaaa ggacaagatg cattccttcc
    27841 ataagaaaca gctactcaaa ctgatttgaa ttaaagctaa caaaaataag attctcattg
    27901 atttcatctt ctctctccct tcctaccaat cattatacta ggagaaaaga gagaactgtt
    27961 tctaatcttc tcaaatgtct ctttaagacg ctaaacaaac actagagact aatactcaat
    28021 gaaaatcaaa gatcaaacta ggtagctagc cacaggttgc tcaaaacact gttttgagat
    28081 tgcagataga gctgacgtga tttgaagaga ttttcgaaga atataaattt gaaatcatga
    28141 aaatccgtca aacgggtggt tgttttgtct cgcacctcac ggagcgagac ggactcagag
    28201 tcacataatt ataaggctga tagtattaat ctaactatca gccttacagg ttatttaacg
    28261 tttcagaaaa actataatgt caagattaac taaacagtat ctagttcctt caaataattt
    28321 tctatcttca tcaacattaa aggattgtta taaatcttac ataactctct tgcttctata
    28381 taataatttt tgacttgttc tctgtctaga aatttggctc cagcatttcc tacaagaata
    28441 agtagaggag ccaattggta gcttgtctgt ctttgtttac agagttcaat cgtttcaaga
    28501 gcttcttgga tggcttcatt atatttttcc tttgatacta ggtagtgagc gtagttgtaa
    28561 cgaactctga tgtagccaaa taaaaactct tgatggtcca aattttttgt ctgatacaac
    28621 tctattaaat gagagtagtt tgcctcatat tcttgttcac gacccactaa ggaatagaaa
    28681 ttagatagag tattcaacgc ctttaaataa atcagagtat ttgaagagac ttttaataat
    28741 atattttcca atgaagaaat tgcctcacac ttactgtcat attgatagaa gtcaataata
    28801 gcttgaatcc attcaaggta agttcggtct tctagtgtta gaaaagtact tcgttcaatc
    28861 tctattctat aaatatattc caaatcgtca taatttctat cgtctaatag tcgagcagac
    28921 aattgcttga aattagagag gttagattta atttcaattt gttcattaaa aaaataatct
    28981 aatggtactt caagtcgttg agagagtttg aacaaaaggt ctgcggaggg aatgaaatgc
    29041 cctctctcaa ttttactaat ctggctttgt tcacaaattc cttctgcaag agtttgttgg
    29101 gagagtctca actcttttct tttcaatctg aatttctctg cgagtgtact cattaaagat
    29161 aataaacctt cctatttg
//
LOCUS       DAVKLW010000025        27255 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKLW010000025 DAVKLW010000000
VERSION     DAVKLW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27255
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.025"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            230..1996
                     /locus_tag="VW324_001074"
     CDS             230..1996
                     /locus_tag="VW324_001074"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9726727.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAILGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            2142..3293
                     /locus_tag="VW324_001075"
     CDS             2142..3293
                     /locus_tag="VW324_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9726728.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMAINGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGYDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            3438..4322
                     /locus_tag="VW324_001076"
     CDS             3438..4322
                     /locus_tag="VW324_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9726729.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(4440..4517)
                     /locus_tag="VW324_001077"
                     /pseudo
     CDS             complement(4440..4517)
                     /locus_tag="VW324_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4721..4973
                     /locus_tag="VW324_001078"
                     /pseudo
     CDS             4721..4973
                     /locus_tag="VW324_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            4961..5251
                     /locus_tag="VW324_001079"
     CDS             4961..5251
                     /locus_tag="VW324_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726730.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(5656..6987)
                     /locus_tag="VW324_001080"
     CDS             complement(5656..6987)
                     /locus_tag="VW324_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9726731.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(7009..8520)
                     /locus_tag="VW324_001081"
     CDS             complement(7009..8520)
                     /locus_tag="VW324_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9726732.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(8731..9678)
                     /gene="arcC"
                     /locus_tag="VW324_001082"
     CDS             complement(8731..9678)
                     /gene="arcC"
                     /locus_tag="VW324_001082"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9726733.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(9843..10856)
                     /gene="argF"
                     /locus_tag="VW324_001083"
     CDS             complement(9843..10856)
                     /gene="argF"
                     /locus_tag="VW324_001083"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9726734.1"
                     /translation="MNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYL
                     AGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDA
                     DEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAF
                     HDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLYI
                     PKV"
     gene            complement(10917..12146)
                     /gene="arcA"
                     /locus_tag="VW324_001084"
     CDS             complement(10917..12146)
                     /gene="arcA"
                     /locus_tag="VW324_001084"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9726735.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            12901..14580
                     /locus_tag="VW324_001085"
     CDS             12901..14580
                     /locus_tag="VW324_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9726736.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGE
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGVEVS
                     GSALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(14620..16704)
                     /locus_tag="VW324_001086"
     CDS             complement(14620..16704)
                     /locus_tag="VW324_001086"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9726737.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQVDWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSTCKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLIFTLSWLPNH"
     gene            16875..18155
                     /locus_tag="VW324_001087"
     CDS             16875..18155
                     /locus_tag="VW324_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9726738.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPLALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            18246..20891
                     /locus_tag="VW324_001088"
     CDS             18246..20891
                     /locus_tag="VW324_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9726739.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQAECAKWGKSTQIGYFPDTFGNMGQAPQILQQSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGADGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVCTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRILVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            20975..21844
                     /locus_tag="VW324_001089"
     CDS             20975..21844
                     /locus_tag="VW324_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9726740.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHCFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAGKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNCNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            21838..23718
                     /locus_tag="VW324_001090"
     CDS             21838..23718
                     /locus_tag="VW324_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9726741.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            24132..24347
                     /locus_tag="VW324_001091"
     CDS             24132..24347
                     /locus_tag="VW324_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726742.1"
                     /translation="MILWSFDFVNAHAHAFFMDNVEWSHADSYFLSFVSDDVEERYTE
                     NVYLDSLSVKQKFKFLFDFGDEWRFEC"
     gene            complement(24528..25376)
                     /locus_tag="VW324_001092"
                     /pseudo
     CDS             complement(24528..25376)
                     /locus_tag="VW324_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(25321..25503)
                     /locus_tag="VW324_001093"
     CDS             complement(25321..25503)
                     /locus_tag="VW324_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9726743.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            25838..>27255
                     /gene="pcpA"
                     /locus_tag="VW324_001094"
     CDS             25838..>27255
                     /gene="pcpA"
                     /locus_tag="VW324_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9726744.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSVI
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLW"
BASE COUNT         8125 a         5296 c         5978 g         7856 t
ORIGIN      
        1 gtttttaaac aacatattga cagtttagaa agcaataatc tatccataga cttaaaagga
       61 ctgaagggaa aaaaggttag aatttctttg agaaacgcag gaattccttt aagtttagca
      121 gaggtagagg tttatactta taagtaattt taaaaattat cacccaggct accgtaaata
      181 taatggagat ggtagtatga aagaaacaga aaaataagag gaaaatagta tgattcaaca
      241 tccacgtatt gggattcgtc cgactattga tggtcgtcgt caaggtgtac gcgaatcact
      301 tgaagtgcaa acaatgaaca tggctaaaag tgtggcagat ttgatttcaa gcacattgaa
      361 atatccagat ggggaacctg tggaatgcgt gatttctcca tctactattg gccgtgtacc
      421 agaggctgca gcttcccatg agttgtttaa aaaatcaaat gtttgcgcaa caattacagt
      481 tacaccatgc tggtgttatg gtagtgaaac tatggatatg tctccagata ttcctcatgc
      541 tattttggga tttaatggga cagaacgccc aggagctgtc tatcttgcag ctgtactagc
      601 ttcacatgct caaaaaggga ttccagcctt tgggatttat ggaagagatg ttcaggaagc
      661 tagtgacaca gatattccag aagatgtcaa agaaaaactt ttacgctatg cgcgtgcagc
      721 tcttgcaact ggcttgatga gagacactgc ttacctatca atgggtagtg tttcgatggg
      781 gattggtggt tctattgtaa atccggattt cttccaagaa tacctaggaa tgcgaaatga
      841 atcggtagat atgacggagt tcacgcgccg tatggaccgt agtatttacg accctgaaga
      901 gttcgaacgt gcgctcaaat gggtgaaaga aaacgtaaaa gaaggattcg accataaccg
      961 tgaagacctt gttttaagcc gtgaagaaaa agatagacaa tgggaatttg ttattaagat
     1021 gttcatgatt ggacgtgact taatggttgg taacccaaga cttgctgagc ttggttttga
     1081 ggaagaagca gttggtcacc atgctttagt agctggtttc caaggtcaac gccagtggac
     1141 agatcatttc ccaaatgggg actttatgga aactttcctc aatactcagt ttgactggaa
     1201 tggtattcga aaaccatttg tatttgcgac agagaatgat tcactaaatg gtgtgtctat
     1261 gctctttaat tatctattaa caaatactcc acaaatcttt gctgatgtgc gtacttattg
     1321 gagcccagag gctgttaaac gtgtaacggg acatacttta gagggtcgtg ctgcagctgg
     1381 cttcttacat ctaatcaact ctggttcttg tacattggat ggtacaggtc aagctactcg
     1441 agatggcaaa cctgttatga aaccattctg ggagttggaa gaaagtgaag tgcaggctat
     1501 gcttgaaaat acagacttcc caccagcaaa ccgcgaatac ttccgtggag gaggattctc
     1561 aactcgtttc ttgacgaagg gggatatgcc agtaacaatg gtacgtctca atcttctaaa
     1621 aggggttggt ccagtgctac aaattgcaga aggttataca cttgaacttc ctgaagatgt
     1681 tcaccatact ttagataatc gtacagatcc aggatggcca actacttggt ttgctccacg
     1741 tttgacagga aaaggtgctt tcaagtctgt ctatgacgtc atgaataatt ggggagctaa
     1801 tcacggagcc ataacatatg gacacattgg agcagacttg attaccttgg cttctatgtt
     1861 gagaattcct gtcaatatgc ataatgtacc tgaggaagat atctttagac ctaaaaattg
     1921 gtccttattt ggaacagaag atctagaatc agcagactat cgtgcatgtc agttgttggg
     1981 accactacat aaataaaact tgtttatata ggaggtgaac ttacgtccct cctatccttt
     2041 taaaaagatt tgttaaacaa ttcacaaata attgaaaacg aatacaaaaa gtaatataat
     2101 gatgttaaat agatagcgcg gaggcgcagg aggaaaatta tatggctaca ttttatgttc
     2161 cggcagtcaa ccttattggt aaaggtgttg taaatgaagt gggtccttat atcaaggaac
     2221 ttggctataa aaaggcactt ttggttacag ataagtacat cgaaggcagt gatattttgc
     2281 ctaaggtttt aaaaccactg gatacagaag gaatcgaata tgtcatcttt agcgatgtag
     2341 agccaaaccc tacttgtaaa aatgtcacag atggggtagc tgctttgcaa gaacatggct
     2401 gtgactttat catcagtctt ggcgggggtt ctccacagga tgcagctagt tgtatttcta
     2461 tcatggctat aaatggtgga aaaccacagg attatgaagg gcttcataag tctgctaaaa
     2521 aaggcttgcc agttgtggct atcaatacaa cggcaggaac ttctgcagaa atcactatta
     2581 actatgtgat tactgatgaa gaacgcaagg ttaagatggt aatggttgac aagaatagcc
     2641 ttgctcttat ctctgttaat gacccggaac tcatgctttc caaacctaaa ggcctgactg
     2701 ctgctactgg tatggatgct ctgactcacg ctgttgaagc tttggtaaca cctggtgctt
     2761 atgatgtaac caagaaactg tctattggtg ctattgagct tatcaaggaa tatcttcctc
     2821 gtgctgtaga aaatggatac gatattgaag cgcgtgaagg tatggtcaat gccatcttcc
     2881 ttggtggtat gagctttaat aatgctggtc ttggttatgt tcactcaatg gctcaccaac
     2941 tcggtgcagt atataatttg ccacatggtg tttgctgtgc catgttgcta ccagttatag
     3001 aacgtgaaaa tgctaaacgt gtaccagaag ctttccgcaa tgttgccaaa gccttgggcc
     3061 ttcatgtgga aggtaaatca gatcaagagt gtgccgatta tgcgattgct gagattgaga
     3121 agctttctga gacagtaggt attcctaaga aattgactga acttggtatt gaagaaaaag
     3181 atttcgactt tgaatacctt tctaagaatg ccttgattga tgcctgcgca ccaggaaatc
     3241 catttatgcc aaccttagaa gaaacgattg ccttttataa agagttattt tagcaagaga
     3301 ttagacgaca ataggacaat tcacctctaa ttattgacat ttcaggaaaa atcgcagtaa
     3361 aaacggcaca gccttcttta aatgtgctat aatactagaa aaatacttgt ggaggttcca
     3421 ttatggcaat atttttcatg atttttctga ttgtttgtgt gctcctattg gtgatagtca
     3481 cactgagtac agtttatgtg gttcgtcagc agtcggtggc gattattgaa cgctttggga
     3541 aataccaaaa ggttgctaat agcggtattc atattcgctt gccttttggg attgactcga
     3601 ttgcagcacg gattcagttg cgcttgttgc aaagtgatat tgtggttgag actaagacca
     3661 aggacaatgt gttcgttatg atgaatgtag cgactcagta ccgtgtcaac gagcagagcg
     3721 tgacagatgc ttactataaa ctcatacgtc cagaatctca gattaaatct tatatcgaag
     3781 atgctcttcg ctcttctgtt ccaaaattaa ccttggatga attgtttgag aaaaaagatg
     3841 agattgccct tgaggttcaa caccaagtag cagaagaaat gaccacttac ggctacatta
     3901 tcgtgaaaac cttgattacc aaggtcgaac cagatgcaga agttaagcaa tccatgaatg
     3961 aaatcaatgc ggcgcaacgt aagcgggtcg cagcacaaga attggcggaa gctgacaaga
     4021 ttaaaattgt cactgcagct gaagccgaag cagaaaaaga ccgccttcat ggtgtgggga
     4081 ttgcccaaca acgtaaggcg attgtggatg gattggcaga gtctatcacc gaactcaagg
     4141 aagccaatgt tggcatgaca gaagaacaaa tcatgtctat cctcttgacc aaccagtatt
     4201 tggatacctt gaataccttt gcctctaaag gaaatcaaac catcttttta ccaaatactc
     4261 caaatggtgt ggatgatatc cgtacacaaa tcttgtcagc ccttcgcgct gagaagaaat
     4321 aatagactaa tactcttcga aaatctcttc aaactacgtc agcgtcgtct tgccgtatag
     4381 atgttactga cttcgtcagt tctatctgca acctcaaaac agtgttttga gctgacttcg
     4441 tcagtcttat ctactacctc aaaacggtgt tttgagcaac ctgtagctag tttcctagtt
     4501 tgctctttga ttttcattga gtataaagag cttggcaaca ggctcttttt gtattgcgtt
     4561 tattaagaaa aacaaaaaac atcgatatac atttaactaa aaattattgt atgttatctc
     4621 ccttaattag gaatgataag ggaataacta gaaagatttg tgaatacaaa ctatttctga
     4681 tatacaaaat atacagtaat aatgaatgat gggagatggg atgaaagaat ttcaatttga
     4741 gagaaagcag cgtttttctt tgaggaaata tgcaatagga gcttgttcgg tcttgctagg
     4801 aacgagttta tttttttgct ggtatgggtg ctcagcctgt acaggataca gaaacgagtt
     4861 cagcactaat ttcaagtcat tatttggatg agcaggattt atctgaaaag ctgaaatctg
     4921 agttgcaatg gtttgaatta gaaaacaagc ttttgaactt atgggagcat taggttacta
     4981 tgaaggattt gttccttatg tttcaaatca atacaaaaac caagctgaag aagaaggcaa
     5041 accgctatct gataaatata ttttcgaaaa aatcttagga aaaacatatg cagccttcaa
     5101 aaaagatcaa attaatgagc gtgtcgagaa attaggtaag ttaaaaccta ttacaataaa
     5161 ttacaacgga aaatcagaag taattgatag taaagaaaaa ttacaagagc ttatgaataa
     5221 agccgttaaa gacgaagtgg ctcaaatata gtaaaatgaa ataagaacag gacaaatcaa
     5281 tcaggacagt caaattgatt tctaaaaatg ttttagaagt agaggtgtac tattctagtt
     5341 tcaacatact ataaaatcag gcaatacaac ggctaaaaag tttgagttta tcgaaatacc
     5401 agtgcaaaaa ctgaaaaaag caatttataa agctcatctc aaagattcag atgactttag
     5461 accagaaaca tccacaccaa atctttttga aagctgtttg aagctttgtc cttgctttct
     5521 tagttcatag atctgaactt tatcttcata actcaatttc ataataaaac accccaaaag
     5581 ttagatttat tctgtctaac ttttggggtg cagttcatta atcggtcgat tttttttgta
     5641 gaaacagttg cctgattaag ttgcaagtcg atagacggct tcggcataaa tatccatagc
     5701 acggtacaaa tcttctagaa cggcacattc atttgcctga tgttctgtct gcttcgctcc
     5761 tgggaataag gcgccgaagg ctacacaatt tggcatggtg cgagcaaaag tggcaccacc
     5821 ggatgaaata gcaggactgt tatcgccagt cttttcttgg tagatttgca tcagtgtgct
     5881 aacgagttta ctgtcttttg cgacgtatag aggcgctaga tagtcaaact cttcgtagcg
     5941 gagttggtag ttttgtgcac atcttgtaag caactctact agttcttcct tgtcagctaa
     6001 gacaggagtc cgaatgtcaa tacggatttc agaacgttca tgattgatca tgagacctgc
     6061 gacattaaag gataggtgac cagaaggttc atctgctata tcaccaaaga tttgtcttcc
     6121 tgtgccgtct tgacctgctt gtgttgcaag aaaactgaga gcagggtgtt cttggagagg
     6181 agcaagaatg gtagctagtc ggatgacagc attgatacct tgactagcat ccttagcatg
     6241 ctttggcact ccgagaaccg ttacggtttg ttcagtggtt tggtaatcat aaccagcttc
     6301 tttgagaccg ttacaaacct gttcatagag gagaccttgg tagttggcct tgtctggtac
     6361 aacgttaaag gcgcctccta cttcaagctc tagttgatcc gatcccgggc catgaagttt
     6421 gacctgtaga agcccttttt cagcataggt cagaggaaaa gatgagtcag gtgcaaagcc
     6481 catactggcc tgttcttcga tggtattgta gcgtgccatg cagcgccaga gggtttcctc
     6541 atcggtacca aagataaagc gtacgcgctt tttgaactga ataccttggt ccagcaagct
     6601 ttttactgca tagagagctg cgagcgaagg gcctttatca tcttggacac cacgtccgaa
     6661 tacccagccg tctttgatag ttgcttcaaa tggcggtgtc tgccaatctg cttcatcacc
     6721 tgatggaaca acatccaaat gacagagaat ggccagaagc tctgctccct gaccgatttc
     6781 tgcatatccg taataacctt tagggtcaag ataggtagtg aaacctatgt ctcgacaaat
     6841 ctctaaagtt ttttctagga catcttggat tgcttgtcca aaaggtgttc cattttctcc
     6901 ttcattgagt actgaaggat aggaaatcaa ggtttttaat gatataagaa attcatcttt
     6961 aacttgattt gttatatcta ttttcatgga atcacctcac tcactatttt ataggaatgg
     7021 aaggaaggtt ccaaggagaa gaagggcgat ggtcactaca ataatagcga ctacgagttt
     7081 gcccatgaat ttccaccaag taccaatgtt gatacgtcca agtgcaagag ctcccatcac
     7141 aataccagat gttggtgcaa tcaagttcaa gacacctgaa gcagattggt aagcagtgat
     7201 aatcaagcta ggacggacat ttacaaattc tccaagtgga gccatgatac ccatagttgc
     7261 gctggcaaga ccagatgaag atgggatcaa gaatgacata ggtagataga agatataagt
     7321 tacaacgata aagacttgtg aagatagacc gctcaagcct tctttacccc agttgaggat
     7381 tgtatcggta atcataccgt cgttcatgat aacttgaata ccacgagcaa tcgctacgat
     7441 caaggcaaca ctgagcaagt cagcagcacc attcatgaag gaagagataa tcttatcttc
     7501 tttaagacca taaataacac caatcaggat acccataaag gcaaagagca tggcgccttc
     7561 tgggaagtac caagtaccta gtgcagaagt agatgaaccg acaatattac caataactgg
     7621 aagaccagtc aaccaagcat taaagtcatc aaaaatggta acgccaaggt ctgtccatgg
     7681 aatgaagctc aataccatca agatgaatgt caacacaaat aagaagagaa ctgatttttg
     7741 tttgctgcta agtgtagatt ctacagatga agattcttct acgttaaagt gtttcaaatc
     7801 ttctttgcga gtactataaa ccagtgactt agtcggatct ttttgaatct tatccgcata
     7861 acggtaaaca aaccaagtac taagagcagt caaggtaacc cagaaaatca gacgaagtac
     7921 gataccgtcc cctgtaccaa ctcccgcagt cgctgaagca atacctgtcg caaatggatt
     7981 cagagtagat gccaaacagc cgatttgaga accgagcaaa ataattgcaa caccagtcag
     8041 gctatcaaaa ccaacggcca tcataactgg cacaaggagt ggatagaagg ccattgtttc
     8101 ttcccccata ccataagttg taccaccgag ggcaaacaaa ggcatcagta ccaaaattaa
     8161 cattttttcg cggcccttat acttcttcac gatagaggca atccctacgt caagagcacc
     8221 agttttgttg acaatgccaa ggaaaccacc aaccataagg atgaagaagg ctacatcaat
     8281 cgctgcgctc gtttctttaa tgagcgaacc ttcctctgga tgagtaccta gcatagcccg
     8341 aatcggtgcc atgaggacat cccaaatccc ttgtggattt tgaggctgag cctcgtaaat
     8401 accttctata aaggcccccg cagggataaa ccaagttagc actgccataa tagcaatgat
     8461 tatcaataat acggtgtaag atgaaggcat cttaaaccct tttttagctt tttcactcat
     8521 ttgtttttcc tcctgatgtc taacaaagtg taaaaaacca actactttct taaataaaca
     8581 tttaagaaaa cgctttctat ttatatttgt tttaccatat taaagtctaa aaaactaata
     8641 tcttttgaaa tattgtacat gttttcaaga ataatttcta atttgatata aagatactag
     8701 aaatagaata catcttatta gtaaaacaac ttatcctttt tcaataattg ttccgctttc
     8761 agattcaatc aaggcgccta gattttcaag ggaagtaata actgcttttc cttctggacg
     8821 accattgaca aaagcgatag ctgcttctac ttttggaagc atgctacctg gtgcaaactg
     8881 atcttgtttg atatattctt ccagctgggc aacattcaca tgttccaatt tttcctggtt
     8941 tggcttgttg tagttaacaa atacataatc tacacctgtc aaaacgatga agaggtctgc
     9001 atcaaccaat tctgccaaac gttgggaagc gaagtcttta tcaataaccg cttcgacacc
     9061 agtcaaatgt ccattgtttt ctttgacgac gggaataccg ccaccacctg cagctacgac
     9121 gacttgacca ttatttaaaa gagtacggat ggtttcaatt tctttgatgt caacaggttt
     9181 tggtgaggca acgaccttac gccagccacg gccagcatct tccttgaaag tcgctccgct
     9241 tttttcggct tctgcttttg cttcttcttc tgaatagaaa ggaccgattg gtttactcaa
     9301 gttaacaaaa gctggatcat ttttatctac gacaacttgc gttacaacag aggcaacatt
     9361 tttttcgatg ccttcatcca agagagcatt ttgcaaagca tttttcaacc agaaaccgat
     9421 gctaccttct gtcatagcga caagtgagtc gagtgggaag gcagggttct tttcagagtc
     9481 tgatgccaaa tgttggagca agagattccc aacttgaggt ccattaccgt gagtgataat
     9541 caaatcatct ccatttttaa tcaattttac aagatgctta gctgtttcaa ctaaagcttc
     9601 ttgttgagcc tttgctgatg ggtcagaaga aagaatcgca tttcctccca aagctactac
     9661 aattttacga tttgccataa attctccttt atcacactca atagaatgcg tttagatttc
     9721 aatttaatga tttttcacat attttataag aaataataga ttaccattac ataaaagagg
     9781 accggactaa agctattagt cgcagccctc atagctgttg gtagacggtt tattatctaa
     9841 aattatactt taggaatata aaggttacca agtgtagcag ccataacagc tttgatagtg
     9901 tgcatacggt tttctgcttg atcgaagtgg cgagcgtact tgctgcggaa gacttcgtct
     9961 gttacttcca tttcttctac accaaatttt tcagcaacgt ctttaccata aacagtgtga
    10021 gtatcgtgga atgctggcaa gcagtgtagg aagatcaagt tttcattgcc tgctttttta
    10081 actaagtcca tattgacttg gtaaggttta agaagagcta cacgttctgc gaatttgtct
    10141 tcttcaccca ttgataccca aacgtctgtg taaagaacgt ctgcatcttt aactgcttca
    10201 tcagcatctt cagtgatgag aacatgtgcg ccactttctt tagcaaatcc ttctgctaat
    10261 tcaacgattt ctttttctgg gaagagttct tttggtgaga agatgtgaac attgacacca
    10321 aggatagcac ctgttacgag caagctgttg gcaacgttgt tacgtccatc accacagtat
    10381 accaatgtca agccttccaa gcgaccgaag ttttcttgaa cagtcaagta gtcagcgagc
    10441 atttgagttg ggtgccattc gtcagttaga ccgttccata ctggaacgcc tgagaattct
    10501 gccaattctt caaccatacg ttggctgaat ccgcggaatt caatcccgtc aaacatacgt
    10561 cccaatactt tagcagtatc ttcagtagat tcttttttac ccaactgaat atcatttgct
    10621 ccgaggtatt ctgggtgagc accaaggtcg atagccgcag ttgtaaaggc tgcacgagta
    10681 cgagtagatg ttttttcaaa taggagagcg atattcttgc cagcaaggta gtggtgttga
    10741 atattgcgtt ttttcaaatc tttcaagtga gctgaaagac caataaggta ttctaactct
    10801 gcacgggtaa agtctttttc tgctaagaag ctgcgtcctt ggaatactga atttatcatt
    10861 ttattatttc ctctttctat tttttacatt ttctattgac gaatgccgaa cagctattac
    10921 acttcttcac gttcaaatgg catagacata caacgaggtc caccacggcc ccgaaccaat
    10981 tcacttccgc gaatcttaat caagcgaagc ccgtattctt ccaaaatctt attggtcacg
    11041 gtattgcggt cataaacaac taccacacca ggtgcgatgg tcaaagtgtt agaaccgtcg
    11101 ttccattgtt cacgcgcagc tgctacgata ttgccaccac cgcaacgaat caaatgaact
    11161 ttttctacac caaggttttg agcaagaagt tcagctaagt cacctttctc ttcaacgatt
    11221 ttaagttttt cgttttcgta agtaactgag taaacgtgaa ggtcgccttc gatttctggg
    11281 tgaatagtga acttgtcata gtctaccata gtgaagacag tatccaagtg catgaattta
    11341 cggttgttag caaattcaaa ggccaaaacc ttcttgaagc caacattttt cttgaagatg
    11401 ttgaccaaaa gtttttcgat agaagctgcg tctgtacgtt gagagatacc tactgcaagg
    11461 acgtctttag aaagaactag ctcgtctcca ccttcgatac gcgtatcttc ttcacggttg
    11521 tagaccaaat ccacttttcc gccatagatt gggtggtatt tgaagatata cttaccgtag
    11581 agtgtttcac ggttacgagt atctgcaaac atgtggttaa gcgatacggc gtttccaatt
    11641 gttgcaaatg ggtcgcgagt gaaatagagg tttggcatcg ggtcaattgc aaatggatac
    11701 tctgattcaa ctaagtcagt tagatcttta gcttcgtcag gaatttctgg caattcaact
    11761 ttttgaatcc cagccattgt tttttcaacc aattcttggt tgtccttgat gccgtgaagc
    11821 aattcacgaa tagcaacctt ggtttgacga tcacggatgt tggcttcgtc taagtattcc
    11881 tcgataaatt gatcgcggat ttctggagag gtcaatgatt cagcagcgag ttgttctagg
    11941 tagagaacct caattccttc atcgcgaaga gcttgggcaa atgcatcatg ttctttttga
    12001 gcatcttcca agaaaggaat atcatcaaaa agaagccttt caagatagtc cggcaacaag
    12061 ttttctaact ccttgcctgg acggtgcaac ataacttttt tcagtttccc aatttctgag
    12121 aagacctgaa ttggatgtga agacatgtat tttcctcctt aaaaattttg acttggcacc
    12181 attttgggta ccgcttacat gtatgttata acgcaattcc taggtaatgt caatggctat
    12241 aatttctcgg aaaataatat aaattatgat gtttagcaca attttatgtt attatacata
    12301 acataggata gaatacatag atacaaatgc ataaacttct ataaaaagga tataaaattt
    12361 ttttaaaaaa tttttcaaaa aatatacaaa tgaccaaaaa gtgtccaatt atcatctggt
    12421 tatcacttgg atctgtataa agagaaatgt ttgtgtgaaa ggataaaaag atgcaacatt
    12481 cttttgaaga tagattttgg agaagcattt cttgttcaat tgtacaaatt atagaatctt
    12541 ttctgagtat ttaggaagag ataattatct tattgaccct ctttttattt tttgttatag
    12601 tcgacgaggt gatagtttag aataatcact tattcacaat ctgtggaaaa gtctggagag
    12661 tgaatggtct tcacacaatg acctgaaaga agcctgagaa taattatgga gagtagtatt
    12721 ctgagaggtg ttagcagaac catatgacag agctgtttga agagggaaca ttgaggagaa
    12781 aaatcctgag cctaccagtt ggagttggaa agagctggct gttagatcat ggtttattat
    12841 ccacaacctg tggataacct tgtgaacaag agaagttgca aaagaaggag atatataacg
    12901 atgaagaaaa tcaaaccgca tggaccgtta ccaagccaga ctcagctagc ttatctggga
    12961 gatgaactag cagcttttat ccacttcggt cctaatacct tttatgacca agaatggggg
    13021 actggacagg aggatcctga gcgctttaac ccgagtcagt tggatgcgcg tgagtgggtt
    13081 cgtgtgctca aggaaacggg cttcaaaaag ttgattttgg tggtcaagca ccacgatggc
    13141 tttgtccttt atccgacagc tcacacagat tattcggtta aggtcagtcc ttggaggaga
    13201 ggagagggcg acttgctcct tgaagtatcc caagctgcca cagaatttga tatggatatg
    13261 ggggtctacc tgtcaccgtg ggatgcccat agtcccctct atcatgtgga ccgagaagcg
    13321 gactacaatg cctattatct ggctcagctg aaggaaatct tatcaaatcc tgcctatgga
    13381 aatggaggga aatttgctga ggtttggatg gatggtgcca gaggagaggg cgcgcaaaag
    13441 gttaattatg aatttgaaaa atggtttgaa accattcgtg acctgcaggg cgattgcttg
    13501 attttttcaa cagaaggcac cagtatccgc tggattggca atgaacgagg gtatgcaggt
    13561 gatccactgt ggcaaaaggt gaatcctgat aagctaggaa cagaagcaga gctggactat
    13621 cttcagcacg gggatccctc gggcacgatt ttttcaatcg gagaggcaga tgtttccatc
    13681 cgtccaggct ggttctacca tgaggatcag gatcctaagt ctctcgagga gttggtcgaa
    13741 atctactttc actcagtagg gcgaggaact ccactcttgc ttaatattcc gccgaatcaa
    13801 gctgggctct ttgatgcaaa ggatattgaa cgactttatg aatttgcgac ctatcgcaat
    13861 gagctctata aagaagattt ggctctggga gttgaggtat ctggttcagc tctttccgca
    13921 gactttgctt gtcgccattt gacagacggc cttgagacca gctcttgggc aagcgatgca
    13981 gacttgccca tccagttaga actcgactta ggttctccta aaacttttga tgtaattgag
    14041 ttaagagaag atttgaagct agggcaacga atcgctgctt ttcatgtgca agtagaggtg
    14101 gatggtgtct ggcaggaatt tggcaggggc ttcacagttg gtcataagcg cctcttgcga
    14161 ggtccgctag tagaggcaca gaaggtgcgc gtgatgatta ccgaggcaca atctattcct
    14221 gtattgacca agatttccct ttataaaact ccgaggctat cacaaacagt agctgttcag
    14281 gggctagcat ttgcagaaaa aagcctagct gtggcaaagg gagaaaatgc ctattttaca
    14341 gttaagcgca gagaatgtag tggtccttta gaagctaaga tttcgattca accggggaca
    14401 ggtgtccatg gtgtcgccta tcaggatgag attcaagtcc ttgcgtttca agctggtgag
    14461 actgaaaaaa gtctgacgct accaaccttg tatttcgcag gagataaaac cttggatttc
    14521 tatctgaacc taacggtgga tggtcagctt gtggatcaac ttcaagtcca agtttcataa
    14581 aagaagaacc tttgcgcgat gcaaaggttc ttttggttat tagtgatttg gtagccagct
    14641 gagggtgaaa attagttgtt cagcttttaa gaggtcttgg tgttgaatag ttgatacgag
    14701 tgttttgttc agtcggcatt ctttgacaaa gttaaaatgg ttgtggtttt gtttagtatg
    14761 gatatccagc catttatctt ctttagctag gtagactcgt agatggtcaa agagagggat
    14821 tccgaggtca tagcttggtt ttcctggaca ggttggataa aatccgagag ctgaccagat
    14881 gtaccaagca gagagactac cattgtcttc atctccagga taggcttccc aacttgggtg
    14941 aaaagctttc tgacggagcg tcttgataag aagggcagtg tagtcagggt aatcgctgta
    15001 gcggaagaga taaggaatgt ggaaactagg ctggttggaa atggctattt gtccaaaagg
    15061 agcagtagcc atctcgctca tttcgtgaat ttcgtaacca tagcctgttg tttcaaagag
    15121 gggagcatct tgacaggctt tcaaaagata gttgctaaag gtttcttttc cacccatcag
    15181 ttggattaag ccagggatgt cgtggagaac gcctaaagta gcttgaatgg cagagcattc
    15241 agcgtagtct cgcccccaac tataaggaga gaagtcaggg tgaaagtttc cttgattgtc
    15301 tcgtgctcgc atgtaacctg tctcagcgtc aaatagctgg cggtaatttt gtgaagcagc
    15361 cttgtaggtt tcagcgattt ctatgttctc tagttttttg gcacagctgg cgatacaaaa
    15421 gtcactatag gcatagtcta gagtatggct aacactttca tggtggtcgg tagagaggta
    15481 acctagttct tggtattggg ctagtccgtg gcggccattg attccgagag ggtcggcttt
    15541 gctggctgtt tcgagcatgg cttggaagag ttctccttct aggtcggggg ccatgtcctt
    15601 gcaggtgcta tctgcgataa taccgtctaa aagtgtacct ggcatcatac cccgttcatc
    15661 tggagccagc cattttggaa ggaaaccagt atcgcggtag ctattgagga aaccttctaa
    15721 aaagcgttga tagtgctccg gtatgataag ggcaaagagg gggaaggtgg tgcggaaggt
    15781 atcccagaaa ccattgttgc taaagaggac accaggcttg acagtaccag tagccagatc
    15841 catgtggatg gcttgccctg attcattaat ctcataaaaa gtctgtggga agaggaagag
    15901 tctgtagagg cagtggtcaa agaaggttcg gtcagcctct cctgtctcta taatgtcaaa
    15961 acgatggagg agattttccc aatccacttg ggcacttgat ttacagctat caaaatcttc
    16021 ttgaggtaga ttgattagag cttgagaagg agagatgaaa gaagtggcta gttgcatctc
    16081 ggtttgacta cttgctaagt caattcgcca gtccccagct tcttggctga tagcaagaat
    16141 atccgtgttc atttgcaggg cagtgaacat cgttagcgaa tttttgttag tttcagtttt
    16201 accttcttgt cgcagggcaa gagtccgctt atctacttgc tctacggtca gttcatctgc
    16261 tgcgtgaaga tagagggaga gggctttgcc ttgcttttgc ttcaaacgaa tagaagcacc
    16321 atagcaagtc ggtgtgagct gggtttcaat ctgataacgc agagaaaaga gcttcaaata
    16381 gtgaggttgg aagcaagctt tatctatatc ataagaagac tggcggtgaa agaggctgtc
    16441 tccacccagt tgactggtga caggtgtcag aaggagccaa gagtagtccc caatccaagg
    16501 actgggctgg tgagttaatc gaatcccctg aaagataggc agatgcggat caaaaaacca
    16561 agatccatcc tggtcactgg tctggggcac aaagtaattc atcccaaaag gcacgcctgt
    16621 gtatggcagg gtatttcccc gagaaaaggc atgcttgttg gtagttccaa agcgggtatc
    16681 gatggtttca agtagtggtt tcatagtctt tcctttagct gtttttctac attatatcag
    16741 aaaaagaggg cctttagaaa aggagtttca aaaataacta ttttgtagaa aaatgactat
    16801 catttgtgta tgtattttct gtctcaaaag ctatatactg aacatgaaac ttgtttgaaa
    16861 gagggaattg cacgatgatt tattcgaaag aaattgttag agaatggcta gatgaagtag
    16921 cagagcgggc taaggaccat ccagagtggg tggatgtttt cgagcgttgc tacaccgata
    16981 ccttggacaa tacggttgaa atcttagaag atggttcaac ttttgtcttg actggggata
    17041 ttcctgccat gtggcttcga gattcgacag cccaactcag accctacctt catgtagcta
    17101 aaagagatgc cctcctgcgt cagaccattg caggtttggt caaacgtcag atgaccttgg
    17161 tactcaagga tccctatgct aactccttca acattgagaa gaactggaaa gggcaccacg
    17221 agactgacca cacagacctt aacggctgga tctgggagcg caagtatgag gtggattcac
    17281 tttgctatcc tttgcagttg gcttatctcc tctggaaaga gactggcgag actggtcagt
    17341 ttgatgagac ttttgtcgca gcgactaagg aaattctcca tctatggaca gttgaacaag
    17401 accacaagaa ctctccttat cgttttgtcc gagatacgga ccgtaaggaa gacaccttgg
    17461 taaatgatgg ctttggacct gactttgcag tgacaggtat gacttggtca gcttttcgtc
    17521 cgagtgacga ttgctgtcag tatagttact tgattccgtc taatatgttt gccgtagtag
    17581 tcttgggtta tgtgcaagaa atctttgtcg aattagacct agctgatagc cagagtgtta
    17641 ttgctgatgc caagcgtctt caggatgaaa tccaagaagg aatcgaaaac tacgcttaca
    17701 ccaccaacag caagggcgaa aagatttacg cttttgaagt agatggccta ggaaatgcca
    17761 gcatcatgga tgatccaaat gtaccaagtc tactagctgc gccctatctg ggctactgtt
    17821 cgatcgatga tgaagtgtat caagccactc gccgtaccat tctgagctct gaaaatccat
    17881 acttctacca aggagaatac gctagcggtc tcggaagttc tcataccttc tatcgctata
    17941 tctggccact cgccctttct atccaaggat tgacaacaag agataaggca gagaaaaaat
    18001 tcttgctgga tcagctggtt gcctgcgatg gtggtacagg tgtcatgcac gaaagcttcc
    18061 acgtagatga tccgaccctc tactctcgtg aatggttctc ctgggctaac atgatgttct
    18121 gtgagttggt cttggattac ttggatattc gctaaggggc tcgctttagc tcaaccgatt
    18181 cttatcagaa tcacaagttt acatttaaaa cgttaaaatt taaatttaga atgaggtttt
    18241 acttcatgga aaatgttgtt gtacatatta tctcacatag tcactgggac cgtgagtggt
    18301 acttgccttt tgaaagccat cgtatgcagt tggtggaatt gtttgacaat ctctttgatc
    18361 tctttgaaaa tgaccctgag ttcaagagtt tccacttgga tggacaaact attgtccttg
    18421 acgactactt acaaattcgc cctgaaaatc gtgacaaggt ccaacgctac attgacgagg
    18481 gcaaacttaa aattggtccc ttttacatct tgcaggatga ctacttgatc tccagtgaag
    18541 ccaatgtccg aaataccttg attggtcaag cagaatgtgc aaaatggggc aaatctactc
    18601 agattggtta tttcccagat acctttggaa atatgggaca agcgcctcaa attcttcaac
    18661 aatcaggcat tcacgtggca gcctttggtc gtggtgtgaa gccgattgga tttgacaacc
    18721 aagtcctcga agatgagcag tttacttccc agttttcaga aatgtactgg cagggtgcgg
    18781 atggtagtcg tgttttaggt attctctttg ccaactggta cagtaatggg aatgaaatcc
    18841 cagttgacaa agatgaggcc ttgaccttct ggaaacaaaa attgtcagat gtgcgtgcct
    18901 acgcttcgac caaccaatgg ttgatgatga acggctgtga ccaccagcct gtacagaaaa
    18961 atctgagcga agccattcgt gtggcaaatg aactcttccc ggatgtaatc tttgttcata
    19021 gttcttttga tgaatatgtt caagctgtag aaggtgcgct tcctgaacac ttatcaactg
    19081 ttacaggcga gttgaccagt caggaaacag atggctggta cacacttgcc aacacttctt
    19141 catcccgcat ttacctaaaa caagccttcc aagaaaatag caacctccta gagcaagtgg
    19201 tagaaccctt gactattatc actggtggac acaaccacaa ggaccagttg acctatgctt
    19261 ggaaaacact tttgcagaat gcgccacatg atagtatctg tggctgtagc gtggacgaag
    19321 ttcaccgcga gatggaaacg cgttttgcca aggtcaacca agtaggaaac tttgttaaaa
    19381 gtaatttgct caacgagtgg aagggtaaaa ttgctacgga taaggctcaa agtgactatc
    19441 tctttactgt cattaacaca ggcttgcatg ataaggtcga tactgtctgc acagtgattg
    19501 atgtggcgac ttgtgatttc aaggaattgc acccaacaga aggctacaaa aagatggctg
    19561 ctcttatctt gccaagttac cgtgtggagg acttggatgg tcgtcctgta gaggctacaa
    19621 tcgaagacct cggagctaat tttgaatacg atttaccaaa agataagttc cgccaagctc
    19681 gtattgctcg acaagtgcgc gtgaccattc cagttcacct agcaccgctt tcttggacaa
    19741 ccttccaatt gctggaagga gaacaagaac accgtgaggg tatttaccaa aacggagtga
    19801 ttgatacacc attcgtaacg gtgagtgtgg atgacaacat cacagtctat gacaagacaa
    19861 ctcacgaagc ctatgaagac tttatccgct ttgaagaccg tggggacatt ggaaacgagt
    19921 atatctattt ccaaccaaaa ggaacagagc caatctttgc agagcttaag ggccacgagg
    19981 tcttggaaaa cacagcttgc tatgctaaaa tcttgctcaa acatgaattg accgtgcctg
    20041 tcagtgcgga tgaaaagcta gaagaagagc aacaaggtat catcgagttt atgaagcgtg
    20101 aggctggacg gtcagaagaa ttgacaaaca ttcctctgga aactgagttg actgtcttcg
    20161 ttgacaatcc acaaatccgc ttcaagactc gctttactaa cactgccaag gatcaccgta
    20221 tccgtatctt ggtcaagact cataacacgc gtccaagcaa tgattctgaa agtatctatg
    20281 aggtggtgac acgaccaaac aaaccagctg cttcatggga aaaccctgaa aatcctcaac
    20341 accaacaagc ttttgtcagt ctgtatgacg atgaaaaagg ggtgactgta tccaataagg
    20401 gattgaatga atacgaaatc cttggggata acaccattgc cgtgaccatt ttgcgtgcat
    20461 caggtgagct aggtgactgg ggctacttcc caacgccaga agcacaatgc ttgcgtgagt
    20521 ttgaagtcga gtttgcactt gaatgccacc aagcccaaga acgcttctca gcctatcgtc
    20581 gtgccaaagc cttgcagaca ccgtttacca gccttcagct tgctagacag gaaggaagcg
    20641 tggttgcgac tggtagcctc ttgagccatt ctgttctcag cataccgcaa gtttgtccaa
    20701 cagcctttaa agtggcggaa aatgaagaag gctatgtcct ccgttactat aatatgagtc
    20761 aagaaaatgt gcgcatatca gaacatcaac aaaccattct tgacttactt gagcgaccat
    20821 atccagttca ttcaggacta ttggctccac aagagattcg tacagaattc atcaaaaaag
    20881 aagaaattta atttcaaaaa gtaaacatca aaagaaagga ggggcgaaaa ggtaagaact
    20941 aactgctgat tcgctccttt ttatggtaaa aacaatgacc attgcaacga ttgatatcgg
    21001 agggactggg attaagtttg ccagtctgac tcctgatggg aaaatactgg ataagacaag
    21061 tatttcaacg cctgaaaact tggaggattt actagcgtgg ctagatcaac gcttgtcaga
    21121 acaggattac agtgggattg ctatgagcgt tccaggtgcg gtcaatcaag agacaggtgt
    21181 gattgatggc ttcagtgcgg tgccctacat ccattgcttt tcttggtatg aggcgcttag
    21241 ctcttatcag ctacctgtcc atttagaaaa tgatgccaac tgcgttggac tcagtgaact
    21301 actagctcat ccagagcttg aaaatgcagc ctgtgtcgtg attgggacag ggattggcgg
    21361 agccatgatt atcaatggta gacttcatcg aggtcgccac ggtctgggtg gagaatttgg
    21421 ttacatgaca acccttgccc ctgctggaaa acttaataac tggtcgcaac tagcatcaac
    21481 tgggaatatg gtacgatacg taattgaaaa atctggtcat actgattggg acggtcgcaa
    21541 gatttaccaa gaggccgcag ctggtaatgc tctttgtcaa gaagccattg agcgcatgaa
    21601 ctgcaatctg gcgcaaggct tgctcaatat ccagtatctg atcgatccag atgtcatcag
    21661 tctgggtggc tctatcagtc aaaatccaga ttttatccaa ggtgttaaga aggctgttga
    21721 agactttgta gatgcctacg aagaatacac ggtcgcacca gttatccagg cctgcaccta
    21781 tcacgcagat gcaaatctct acggtgccct tgtcaactgg ctacaggagg aaaagcaatg
    21841 gtaagattta caggacttag tcccaaacaa acgcaagcta ttgaggtttt aaaaggtcac
    21901 atttctctac cagatgtgga agtggctgtc actcagtctg accaagcctc tatctctatc
    21961 cagggtgagg aaggtcacta tcaattgacc taccgcaaac ctcaccaact ttatcgtgcc
    22021 ttgtccttgt tggtaacagt tctagcagaa gctgataaag tagagattga ggaacaagca
    22081 gcttacgaag atttggctta catggttgac tgttctcgaa atgcggtgct gaatgtggct
    22141 tctgccaagc agatgattga ggtcttggct ctcatgggct actcaacctt tgagctttac
    22201 atggaagaca cttaccagat tgaaggacag ccttactttg gctatttccg tggagcttat
    22261 tcagcagagg agttgcagga aatcgaagcc tatgctcaac agtttgacat gacctttgta
    22321 ccatgcatcc agaccttggc ccacttgtcg gcctttgtca aatggggtgt caaggaagtg
    22381 caggagctcc gtgatgtaga ggacattctt ctcattggcg aagaaaaggt ttatgacctg
    22441 attgatggca tgtttgccac gttgtctaaa ctgaagactc gcaaggtcaa tatcgggatg
    22501 gacgaagccc acttggttgg tttgggacgc tacctgattt tgaacggtgt tgtggatcgt
    22561 agtctcctca tgtgccaaca cttggagcgc gtgctggata ttgctgacaa atatggtttc
    22621 cactgccaga tgtggagtga tatgttcttc aaactcatgt cagcggatgg ccagtacgac
    22681 cgtgatgtgg aaattccaga ggaaactcgt gtctacctag accgtctcaa agaccgtgtg
    22741 actctggttt actgggatta ttatcaggat agcgaggaaa aatacaaccg taatttccgc
    22801 aatcatcaca agattagcca tgaccttgca tttgcagggg gagcttggaa gtggattggc
    22861 tttacacctc acaaccattt tagccgtcta gtggctatcg aggctaataa agcctgccgt
    22921 gccaatcaga ttaaagaagt catcgtaacg ggttggggag acaatggtgg tgaaactgcc
    22981 cagttctcta tcctaccaag cttgcaaatc tgggcagaac tcagctatcg caatgaccta
    23041 gatagtttgt ctgcgcactt caagaccaat actggtctaa cggttgagga ttttatgcag
    23101 attgaccttg ccaacctctt accagaccta ccaggtaatc tcagtggcat caatcccaac
    23161 cgctatgtct tttatcagga tgttctctgt ccgatacttg accgacacat gacacctgag
    23221 caggacaagc cacacttcgc tcaggcggcg ggaactattg ctgacattaa agaaaaagct
    23281 ggaaactatg cttatctctt tgaaactcag gcccagttga atgctatttt aagtagcaaa
    23341 gtagatgtgg gacgacgcat tcgtcaggcc taccaagcgg atgataaaga aagtttacaa
    23401 caaatcgcca gacaagaatt accagaactt agaagccaaa ttgaagactt ccatgccctc
    23461 tttagtcacc aatggttgaa agaaaacaag gtctttggtt tggatacagt tgacatccgt
    23521 atgggcggac tcttgcaacg catcaaacga gcagaaagcc gtatcgaggt ttatctggct
    23581 ggccagcttg accgcatcga cgagctggaa gttgaaatcc taccatttac tgacttctac
    23641 gcagacaagg atttcgcagc aactacagct aaccagtggc ataccattgc gacagcgtcg
    23701 acgatttata cgacttaata cttttcgaaa atctcttcaa accacgtcag cttccatcta
    23761 caacctcaaa acagtgtttt gagcagcctg cagctagctt cctagtttgc tctttgattt
    23821 tcattgagta taaaaacaag aacaccttgc ttggcgcagg gtgtttcgcg tgaaacagaa
    23881 gaattatctg gtttcaaatg ctacagttag acaaacttat gataaaatag cagaaagtga
    23941 atgtttccta agagcaattc tatcgatgag caattggagg tattatgcta cacttaaaat
    24001 tagtaaaaca agaaatagaa gctgaaaagc cagcatctgt agaagcctgg gtcatttccg
    24061 tcaaatttaa aaaaggttgc taccgacata tatagattcc aaaaacaaaa acgttagcgg
    24121 aactagcaga tgtgatttta tggagtttcg attttgtaaa tgctcatgct cacgcatttt
    24181 tcatggataa tgttgagtgg agtcatgcag attcttactt tcttagcttt gttagtgacg
    24241 atgttgaaga acgttacaca gaaaatgtct atctggatag cctaagtgtc aaacaaaaat
    24301 ttaagtttct tttcgacttc ggtgatgaat ggcgttttga atgctaagtg ctgagagaaa
    24361 tcgagacaga ggacgaagaa gcttatctcg tacgttcggt tggaacgtcg ccagaacaat
    24421 atccagatta tgatggtttt gactatgaag aatggtaaaa ttgaaatcta aaaaacaatt
    24481 ggaatcagtc tgtgtaggct tagtatttca atagacttcc tgcaaaacta gaatcctagt
    24541 tcatgattga taataccagc aatcaaattc attcgtaatc cgaagcgttt acgatgattt
    24601 cgatagattg ttgaaaacat tttaaacgtt tttactttgg caaagatgtt ctcaaccttg
    24661 cttctctcct tggatagcgc atggttacag gctttatctt cagctgttag cggcttgagt
    24721 ttgctggatt tacgtggagt ttgtacttga ggatatatct tcatgagtcc ttaataacca
    24781 ctgtcagcca agattttacc agcttgtccg atatttctac gactcatttt gaacaacttc
    24841 atatcatgac aatagttcac agcgatatcc aaagaaacaa ttctcccttg acttgtgaca
    24901 atcgcttgag ccttcatagc gtgaaatttc tttttaccag aatcattcgc taattgtttt
    24961 tttagggcga ttgattttta cttccgtcgc atcaatcatt accgtgtcct cagaactgag
    25021 aggagttctt gaaaccgtaa aaccactttg aacaagagtt acttcaaccc attggctccg
    25081 acggataaag ttgctttcgt gaataccaaa atcagccgca atttgttcat aagttcgata
    25141 ttctcgcact attgaagagt gggcataaga aggtcttcta ggcttaattt aggttttcgt
    25201 ccaccttttg cgtgtttaag ttgataagct gtttttaata cagctaacat ctcttcaaaa
    25261 gtggtacgct gaacaccaac aagacgctta agtcgtgtat cagttaattg tttacttgct
    25321 tcataatttc gcagggagtc tattgactct ttggtaggtg tcaatgtttt tttcatctat
    25381 cccgagaatt attttcccac catttgtatt tgcaaatgct gagtaggttt cccagaaaga
    25441 ctctggaaga ttgtttttag cttttttgta ttctaaatca accccttcaa attttaagtc
    25501 catatttttc ctttacatct gttttttgtg gttctggtat ttgttcaagt tgagtgataa
    25561 tatagcgaat tgaatttcga gagtttttac tcagttaatt tcttttttaa cccactttaa
    25621 tttctttttt aatacgggtt aaaaaagaaa ttaaagtggg ttaatttttc ttgacttaaa
    25681 atttaaaaat gatatactgt gtttatggag agatgacaat ttataaaatg tactactcta
    25741 ttctactaga taatgattga ataaaacttt ttataacaac ggctagcaaa gttataattt
    25801 tatatctatt agaaaataaa tatgtaagga aatttaaatg aaaaaaacta caatattatc
    25861 attaactaca gctgcggtta ttttagcagc atatgtccct aatgaaccaa tcctagcaga
    25921 tactcctagt tcggaagtaa tcaaagagac taaagttgga agtgttattc aacaaaataa
    25981 tatcaaatat aaggttctaa ctgtagaagg taacatagga actgttcaag tgggtaatgg
    26041 agttactcct gtagagtttg aagctggtca agatggaaaa ccattcacga ttcctacaaa
    26101 aatcacagta ggtgataaag tatttaccgt tactgaagta gctagtcaag cttttagtta
    26161 ttatccagat gaaacaggta gaattgtcta ctatcctagc tctattacta tcccatcaag
    26221 cataaaaaaa atacaaaaaa aaggcttcca tggaagtaaa gctaaaacta ttatttttga
    26281 caaaggcagt cagctggaga aaattgaaga tagagctttt gatttttctg aattagaaga
    26341 gattgaattg cctgcatctc tagaatatat tggaacaagt gcattttctt ttagtcaaaa
    26401 attgaaaaag ctaacctttt cctcaagttc aaaattagaa ttaatatcac atgaggcttt
    26461 tgctaattta tcaaatttag agaaactaac attaccaaaa tcggttaaaa cattaggaag
    26521 taatctattt agactcacta ctagcttaaa acatgttgat gttgaagaag gaaatgaatc
    26581 gtttgcctca gttgatggtg ttttgttttc aaaagataaa actcaattaa tttattatcc
    26641 aagtcaaaaa aatgacgaaa gttataaaac gcctaaggag acaaaagaac ttgcatcata
    26701 ttcgtttaat aaaaattctt acttgaaaaa actcgaattg aatgaaggtt tagaaaaaat
    26761 cggtactttt gcatttgcgg atgcgattaa acttgaagaa attagcttac caaatagttt
    26821 agaaactatt gaacgtttag ccttttacgg taatttagaa ttaaaagaac ttatattacc
    26881 agataatgtt aaaaattttg gtaaacacgt tatgaacggt ttaccaaaat taaaaagttt
    26941 aacaattggt aataatatca actcattgcc gtccttcttc ctaagtggcg tcttagattc
    27001 attaaaggaa attcatatta agaataaaag tacagagttt tctgtgaaaa aagatacatt
    27061 tgcaattcct gaaactgtta agttctatgt aacatcagaa catataaaag atgttcttaa
    27121 atcaaattta tctactagta atgatatcat tgttgaaaaa gtagataata taaaacaaga
    27181 aactgatgta gctaaaccta aaaagaattc taatcaggga gtagttggtt gggttaaaga
    27241 caaaggttta tggta
//
LOCUS       DAVKLW010000026        26299 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKLW010000026 DAVKLW010000000
VERSION     DAVKLW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.026"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            84..1844
                     /locus_tag="VW324_001095"
     CDS             84..1844
                     /locus_tag="VW324_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9726745.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIG
                     RSAMVTKLLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSG
                     VSGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNDQTGQQDTGDNDADY"
     gene            1831..3147
                     /locus_tag="VW324_001096"
     CDS             1831..3147
                     /locus_tag="VW324_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9726746.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            3322..4308
                     /locus_tag="VW324_001097"
     CDS             3322..4308
                     /locus_tag="VW324_001097"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9726747.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4372..4443
                     /locus_tag="VW324_001098"
     tRNA            4372..4443
                     /locus_tag="VW324_001098"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4405..4407,aa:Arg,seq:ccg)
     gene            4626..5324
                     /locus_tag="VW324_001099"
     CDS             4626..5324
                     /locus_tag="VW324_001099"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9726748.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTYLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5315..8854
                     /gene="smc"
                     /locus_tag="VW324_001100"
     CDS             5315..8854
                     /gene="smc"
                     /locus_tag="VW324_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9726749.1"
                     /translation="MYLKEIEIQGFKSFADKIKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQDEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRQQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8851..9645
                     /locus_tag="VW324_001101"
     CDS             8851..9645
                     /locus_tag="VW324_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9726750.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9645..10463
                     /locus_tag="VW324_001102"
     CDS             9645..10463
                     /locus_tag="VW324_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9726751.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVMPMTNDEEAVAWAIEEYVLKEN"
     gene            10467..11756
                     /gene="ftsY"
                     /locus_tag="VW324_001103"
     CDS             10467..11756
                     /gene="ftsY"
                     /locus_tag="VW324_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9726752.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11800..13287)
                     /locus_tag="VW324_001104"
     CDS             complement(11800..13287)
                     /locus_tag="VW324_001104"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9726753.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(13436..14176)
                     /locus_tag="VW324_001105"
     CDS             complement(13436..14176)
                     /locus_tag="VW324_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9726754.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14176..16341)
                     /locus_tag="VW324_001106"
     CDS             complement(14176..16341)
                     /locus_tag="VW324_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9726755.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            16511..17464
                     /locus_tag="VW324_001107"
     CDS             16511..17464
                     /locus_tag="VW324_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9726756.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            17541..19529
                     /gene="uvrB"
                     /locus_tag="VW324_001108"
     CDS             17541..19529
                     /gene="uvrB"
                     /locus_tag="VW324_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9726757.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            19540..20103
                     /locus_tag="VW324_001109"
     CDS             19540..20103
                     /locus_tag="VW324_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726758.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            20069..20557
                     /locus_tag="VW324_001110"
     CDS             20069..20557
                     /locus_tag="VW324_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726759.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            20631..21098
                     /locus_tag="VW324_001111"
     CDS             20631..21098
                     /locus_tag="VW324_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9726760.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(21095..21610)
                     /locus_tag="VW324_001112"
     CDS             complement(21095..21610)
                     /locus_tag="VW324_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9726761.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(21622..22149)
                     /locus_tag="VW324_001113"
     CDS             complement(21622..22149)
                     /locus_tag="VW324_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9726762.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(22431..22994)
                     /locus_tag="VW324_001114"
     CDS             complement(22431..22994)
                     /locus_tag="VW324_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9726763.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(22978..23529)
                     /gene="coaC"
                     /locus_tag="VW324_001115"
     CDS             complement(22978..23529)
                     /gene="coaC"
                     /locus_tag="VW324_001115"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9726764.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(23541..24230)
                     /gene="coaB"
                     /locus_tag="VW324_001116"
     CDS             complement(23541..24230)
                     /gene="coaB"
                     /locus_tag="VW324_001116"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9726765.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKNLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            24460..26130
                     /locus_tag="VW324_001117"
     CDS             24460..26130
                     /locus_tag="VW324_001117"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9726766.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
BASE COUNT         8066 a         4705 c         5853 g         7675 t
ORIGIN      
        1 ataaggcaac tgttggtgaa aagtttaatg ataaattaaa agaagagttt ctccaggaat
       61 ggcctttgga taggatttta acaatgtcaa tcgatgaata tgtaatagga aagggacagc
      121 aaaataagtc tttatgctac gctcttgaga agggaaaata caaaaatcta tttcttggaa
      181 tttctggtgg ctcagcttca aaatttggta tttattggaa taaaaaaaca aacaaatata
      241 aagatcaagc taataatgag atttcagagt tggatcagcg attttcaaaa ttaaaatcag
      301 atttgtatga aattatcaaa gaaggtattc gttttaactt tgaaaatcct atttttgata
      361 tgaaaagatc aacaaatgaa tttattggtc gttctgctat ggtgacaaaa ttactttgta
      421 tctatactga gggagatcct ttctttggtg taaatattaa tagtcagaaa gaattttgga
      481 accactttgt ttctcagaca aatcaaggtg gaccttatct gcaaaatcat aaaataattg
      541 aactagtgtc caaaacttat cctgagttgg agccatcgaa attaggaact atgctttttg
      601 agtattctaa gctttttatg gaaaataagg aagacaatag tacaatggat tcatcaaaca
      661 attttcgtca tcaattaact caatctctat taaagtctcc aaacctcatc ctccgtggtg
      721 ctcctggcac aggaaagact tatcttgcta aagaaattgc caaagaatta acggatggca
      781 acgaagacca aatcggattt gtacaatttc acccatccta tgattatacg gattttgtag
      841 aaggtttaag accagtatca aatggggatg gagctattga gtttaggcta caggacggta
      901 tttttaaaga tttttgtcag aaagcaaaag aaacccaatt gattggagga caagataatt
      961 ttgatgaggc ttgggattct tacttagaat atataaatgt tgctgaagaa aaagaatata
     1021 taacaaaaac atcttactta tctattaata gtagacaaaa tttgtcagta aattatgata
     1081 gtggtgtttc aggatggtca ctacctagca aatatgttta cgagttgtat aaagataaaa
     1141 attataataa gcaagaatac tacaaaagtg gtggaaaaac tgtcctagaa acattgagaa
     1201 agagatttgg tttgaaagac tatgtttccc caacagaaat tgatactgat aagaaattcg
     1261 tcttcatcat cgatgaaatc aatcgtgggg agatttctaa gatttttggc gaactctttt
     1321 tctctatcga ccctggctat cgtggtgaaa aaggaagtgt ttctacccaa tatgcaaatc
     1381 tacacgaaac tgatgaaaag ttctatatcc ccgaaaatgt ttacatcatc ggaactatga
     1441 atgatattga tcgttcagtg gatacctttg attttgctat gcgtcgtcgt tttcgttttg
     1501 ttgaagttac tgccgagggt caagttggca tgttggataa agagctgaat atccatgcag
     1561 aagaagcaaa aattcgtcta agaaacttga acgctgctat cgaaaatgtt caggaattaa
     1621 acagtcatta tcatattgga ccaagttatt ttcttaagtt gaaggatgta gattttgact
     1681 atgaattact ctggtctgat tatattaagc ctctcctaga agactacttg cgaggttctt
     1741 atgatgaggt tgaaactttg gaaactttga aaaaagcatt tgaactgaca aataatgatc
     1801 aaactggtca acaagatact ggtgataatg atgcggatta ctgataatca atacaagatt
     1861 attaaagaaa aatttgttga agaatatcct aaactaagca atcttctttt agacagaacc
     1921 ttggaaagtc tatcccaaga tgaacgtatt ttcatttttc caaatgattt gactcatact
     1981 cctgatttgg ataaggacca aaagattttt gaaacagtca atcagaaaat caagacaggg
     2041 aacgtgattg gttttcttgg atatggtcag gaaagattaa cgatttcctc acgattttct
     2101 gatgagagta atgaccattt tttgcattat ctcttaaaca aggttcttca tatcaatctc
     2161 actagtttag atgttgcttt gtctcgtgaa gagaggcttt atcaactttt gatgtatctc
     2221 ttccccaagt atctacaagc tgctattcga aaaggtcttt ataaggaata ccagagattt
     2281 tctcataacg acagtcatgt taagggagtg attgatgtaa gaaaccatct caagaaaaat
     2341 cttcctttca cgggaaatat tgcctacaca acgagagagt tcacctatga taatcccctc
     2401 atgcagttgg tccgtcacac tattgaatac attaagaatc agaaaagcat tggtcaaggg
     2461 gtactagata atctctcaac tagtcgtgaa aacgtatctg aaatcgtgcg tgtaacgccc
     2521 tcttataaac tagctgatcg tgctaagatt attcggggaa atcaatctaa acctatacgt
     2581 catgcatact ttcacgagta cagaaactta caagaacttt gtctgatgat cctaaaccaa
     2641 gaaaagcacg gtttagggta tcaagatcaa aaaatctatg gtattctctt tgatgttgcc
     2701 tggctttggg aagagtatgt ttacaccttg ttgccaaaag gttttgtaca tcccagaaat
     2761 aaggataaga cggatggaat ttcagtattt tctgttggga aacgaaaagt atatccagat
     2821 ttttatgaca gagaacgaaa gattgttcta gatgcaaaat ataaaaaact ggaattgact
     2881 gaaaaaggaa tcaaccgtga ggacttattc cagctgattt cctattctta tattttaaaa
     2941 gctgagaagg ctggacttgt ttttccgagc aaggacaagg ttattgataa tgagatggga
     3001 aacctagcag gttatggagc tttgttgaag aaatggtcta tccaaattcc tcagaagtct
     3061 tcatcttata gtgcattttg taaaatgatg gaaaattcag aagaaaattt taaagcgatt
     3121 attgatgaag aagtggggag aaaataattt cctctctttc ttttattttt aacacaagta
     3181 aaacccgaac aatatatttc aattcataaa ataatttgac aaaaactgta ctttggttta
     3241 taataaatta aggaaacgtc ggaaaagacg tagtgatgcg agagcatagg taggtcatta
     3301 caaaagaaac gagacatcga tatgttaaat gaatttccaa tttttgatta cgaagatatt
     3361 caattgattc caaataaatg tgtcattaaa agccgtgcag aagcggatac aagtgtcact
     3421 ttaggaaatc acacctttaa actacctgtt gtgccagcga atatgcagac gattttggat
     3481 gaaaatgtag cagagcaact ggctaaaggt ggttacttct acattatgca ccgttttgat
     3541 gaggcaggac gcattccttt tattaaacga atgcacgatc aagggctcat tgcttctatc
     3601 tctgtcggtg ttaaggacta tgagtatgat ttcgttagac agctcaagac tgatgctcct
     3661 gagtatatca ctattgatat tgctcatggt catgcggata gcgtgatttc tatgattcaa
     3721 cacatcaaga aagaattgcc agataccttt gtcattgctg gaaatgtggg aacaccagaa
     3781 gctgtgcgtg aattggaaaa tgctggtgcg gatgctacta aggtcggaat cggtcctggt
     3841 aaggtttgta tcaccaaggt taagactggt tttggtacag gtggttggca gttggctgct
     3901 ctacgctggt gtgccaaggc tgcgcgtaaa ccgattatcg ctgatggagg aattcgtact
     3961 cacggtgata ttgccaagtc tatccgcttc ggtgctagca tgatcatgat tggttccctc
     4021 tttgcaggac acattgaaag tccagggaaa acgattgaag tcgatggtga acagttcaaa
     4081 gaatattatg gttcagcctc acaatatcag aaaggtgctt acaaaaatgt ggaaggcaaa
     4141 cgtatcttgc ttcctgctaa aggtcatctg caagacactt taactgagat ggaacaagac
     4201 cttcaaagtg ctatttctta tgcaggtgga cgtcaggttg ctgaccttaa acacgttgat
     4261 tatgtgatcg tgaaaaactc aatctggaat ggggatgctt cccactaaga cggggtcttt
     4321 gactaaaaaa cttgttatta gagcaaattt ctgttataat aaaacaagtt tccaccctta
     4381 gtgtaatgga tatcacgtaa gattccggtt cttgagatgg gggttcgatt ccctcagggt
     4441 ggatgtaaac atcctaaaaa gcctttaata gggctttttt ctttacactg ctctaaattt
     4501 gccgctaaaa ctgagaatct ctgtctaaag aattcccaaa tgctttccag tcccgtttta
     4561 gagtgactca gggggctttt tttgatataa taaaaaggac tgttatcagt tagaaagagg
     4621 ttggtatgaa agaattacaa actgtactaa agaaccattt tgcaatcgaa tttacagaca
     4681 aaaatttact ggaaactgcc tttactcata cgagttatgc caatgagcac cgcctcttaa
     4741 aaatttcaca caatgaacgc ttggaatttt taggagacgc tgttctacag ttattgattt
     4801 cagaatatct gtataaaaaa tatcctaaaa agcctgaagg tgacctatca aaactccgtg
     4861 ctatgattgt ccgtgaggag agtttagctg gttttgcgcg tgattgccag tttgaccagt
     4921 ttatcaagtt gggtaaaggg gaagaaaaat caggtggtcg caatcgtgac accattcttg
     4981 gtgatgcctt tgaagccttt cttggtgccc ttcttttgga taaggatgtg gccaaggtca
     5041 aggaatttat ctatcaagtc atgattccta aggttgaagc aggcgagttt gagatgatta
     5101 cagactataa aacctatctc caagagttgc ttcaggtcaa tggtgatgtg gctattcgtt
     5161 atcaggtgat ttctgaaaca gggcctgctc acgataaggt ctttgatgta gaagttcttg
     5221 ttgaaggtaa gagcatcggt caaggccaag gtcgttctaa gaaattagca gagcaggaag
     5281 ctgccaaaaa tgccgttgag aaggggctgg attcatgtat ttaaaggaaa tcgaaattca
     5341 ggggttcaag tcttttgctg ataagatcaa ggtcgttttt gaccaaggtg tgacggcagt
     5401 tgttggaccc aatggatctg gaaagtccaa tattacagaa agtctgcgtt gggctttggg
     5461 ggagtctagt gtcaagagtc tccgtggggg caagatgccg gatgtcatct ttgctggaac
     5521 cgaaagtcgc aaaccgctca attatgcttc tgtagttgtg actctggata atcatgacgg
     5581 atttatcaag gatgcaggtc aagaaatcag ggtggaacgc catatctatc gtagtggaga
     5641 tagcgaatac aagattgacg gcaagaaagt ccgtctgcgt gatattcatg acctcttctt
     5701 ggatactgga ttgggacgag attccttctc tattatttcc caagggaagg ttgaggagat
     5761 ttttaattcc aagtctgagg aacgacgagc tatttttgaa gaagctgctg gagttttaaa
     5821 atacaagact cgcagaaaag aaaccgagag taaactgcag caaactcagg ataatctgga
     5881 ccgcttagag gacattatct acgagttgga taatcaaatc aagcctcttg agaagcaagc
     5941 tgagaatgcc cgtaagtttt tagacttgga aggacaacgt aaggctattt atttagacgt
     6001 tctggttgct caaatcaagg aaaataaggc agaactagag tcgacagaag aagagctggc
     6061 tcaggttcaa gaactcttga tgagttatta ccaaaagcgt gaaaaattag aagaagaaaa
     6121 tcaaactctt aaaaagcaac gccaagattt acaggatgaa atggccaaag accaaggcag
     6181 tttgatggac ttaactagtc tgattagtga tttagaaaga aaattagccc tatcgaaact
     6241 ggagtccgag caagtggcct tgaatcaaca ggaggcacaa gctcgtttgg ctgctttgga
     6301 ggataagaga aattcactca gcaaagaaaa gtatgataaa gaaagctctt tagctctgtt
     6361 agagggaaat ctagtccaaa ataatcaaaa actcaatcgt ttagaagctg aattgctggc
     6421 tttctcagac gatcctgatc agatgattga gctcttacgt gaacgctttg tagctctttt
     6481 acaagaagaa gcggatgtct caaaccagtt gacccgtatt gagaatgagt tggaaaatag
     6541 tcgtcagctt tctcaaaaac aagcagatca actagaaaag ctgaaagagc aattggctat
     6601 agctaaagag aaggctagtc agcaaaagga agagcttgaa actgccaagg agcaggttca
     6661 gaaattattg gctgactacc aagctattgc caaggagcaa gaggagcaga aaacttccta
     6721 tcaagctcaa caaagccaac tctttgaccg tctggataat ctcaaaaaca agcaggccag
     6781 agctcaaagt ttggaaaata tcctgagaaa tcatagtaac ttttatgcag gtgttaagag
     6841 tgttctccaa gaaaaagatc gcctaggtgg gattattggt gcagtcagtg agcatctgac
     6901 ctttgatgtt tattatcaaa ctgccctaga gattgcctta ggggcaagta gccagcatat
     6961 catcgtagaa gatgaagagt cggcaaccaa ggctattgac ttccttaaac ggaacagagc
     7021 cggtcgtgca acctttcttc ctttaaccac tatcaaggcg cgtacgattt ctagtcagaa
     7081 ccaagatgct atcgctgtaa gcccaggttt ccttgggatg gcagatgagt tggtgacttt
     7141 tgatactaga ctggaagcca ttttcaagaa cttgctagct acgacggcta tctttgatac
     7201 cgtagaacat gcgcgtgcag cagctcgcca agttcgttat caggttcgta tggtgacatt
     7261 ggatgggaca gaattacgca cgggtggttc ttacgcaggt ggagccaatc gtcagaacaa
     7321 cagtattttc atcaagccag aactggagca attacaaaaa gaaattgctg aagaagaagc
     7381 aagtttgcgt tcagatgaag tgagtttgaa gaacttgcaa gatgagctag ctagattgac
     7441 agaaagatta gaagccatca aatctcaggg agagcaggct cgtattcagg agcaaggctt
     7501 gtccctcgcc tatcagcaga ccagtcagca agttgaagaa ctggaaactc tttggaaact
     7561 ccaagaagag gaaatagatc gtctttccga gggagattgg caagcggata aggaaaaatg
     7621 ccaagagcgt cttgctgcaa tcgccagtga caagcaaaat ctggaagctg agattgaaga
     7681 gattaagtct aacaaaaatg ccatccaaga acgctatcaa aacttgcagg aagagctagc
     7741 gcaagctcgt ttgcttaaga cagaactgca agggcaaaaa cgttatgaag ttgctgatat
     7801 tgaacgctta ggcaaggaat tggacaatct tgattttgaa caagaagaaa tccagcgcct
     7861 tcttcaagaa aaggttgata atcttgagaa ggttgataca gaattgctca gtcaacaggc
     7921 ggaagaatcc aaaactcaga aaacaaatct ccaacaaggt ttgattcgcc agcagtttga
     7981 gttggatgat atagaaggtc aactggatga tattgccagt catttggatc aggctcgcca
     8041 gcagaatgag gagtggattc gcaagcaaac acgtgctgaa gccaagaaag aaaaggtcag
     8101 cgaacgcttg cgccatctac aaagtcaatt aacagaccag taccagatta gctatactga
     8161 agctctagaa aaagcgcatg aattggaaaa cctcaatctg gcagagcaag aagttaagga
     8221 tttagagaag gctattcgct cactgggtcc tgtcaatata gaagctattg accagtacga
     8281 agaagttcac aaccgtctgg actttctaaa tagtcagcga gatgatattt tgtcagcgaa
     8341 aaatctgctc cttgaaacca ttacagagat gaatgatgag gttaaggaac gctttaaatc
     8401 aacctttgag gccattcgtg agtcctttaa agtgaccttc aagcagatgt ttggcggagg
     8461 tcaggcagac ttgatattga ctgagggcga ccttttaaca gctggtgtgg agatttctgt
     8521 tcaacctcca ggtaagaaaa tccagtcgct taacctcatg agtggtggtg aaaaagctct
     8581 atcggctctt gccttgctct tctccattat tcgtgtcaag actatcccat ttgtcatctt
     8641 ggatgaggta gaggctgcgc tggacgaagc caatgttaaa cgttttgggg attacctcaa
     8701 ccgctttgac aaggacagcc agttcatcgt cgtaacccat cgtaagggaa ccatggcagc
     8761 ggctgattct atctatggag tgaccatgca agaatcgggg gtctcaaaaa ttgtttcggt
     8821 taagttaaaa gatttagaaa gtattgaagg atgacaatta aactagtagc aacggatatg
     8881 gacggaacct tcctagatgg gaatgggcgc tttgatatgg atcgcctcaa gtctctcttg
     8941 gcttcctata aggaaaaagg gatttacttt gcggtggctt cgggtcgggg atttctgtct
     9001 ctggaaaaat tatttgctgg tgttcgtgat gacattattt tcatcgcgga aaatggcagt
     9061 ttggtagagt atcaaggtca ggacttgtat gaagcgacta tgtctcgtga cttttatctg
     9121 gcaacttttg aaaagctgaa aacgtcacct tatgtagata tcaataaact gctcttgacg
     9181 ggtaagaagg gttcatatgt tctagatacg gttgatgaga cctatttgaa agtgagtcag
     9241 cattataatg aaaatatcca aaaagtagcg agtttggaag atatcacaga tgacattttc
     9301 aaatttacaa ccaacttcac agaagaaacg ctagaagctg gtgaagcttg ggtcaatgat
     9361 aatgtccctg gtgtcaaggc tatgacaact ggctttgaat ctattgatat tgttctggac
     9421 tatgtcgata agggtgtagc tattgttgaa ttagctaaaa aacttggtat cacaatggat
     9481 caggtcatgg cttttggaga caatcttaat gacttacata tgatgcaggt tgtgggacat
     9541 cctgtagctc ctgaaaatgc acgacctgaa attttagaat tagcaaagac tgtgattggt
     9601 caccataagg aacggtcggt tatagcttat atggagggct tataatggca gatataaaat
     9661 tgattgcatt ggacttggac gggaccttgc tgactactga taaaaggctg acggatcgta
     9721 ccaaggaaac cttgaaagct gcgcgtgatc gtggtatcaa ggtcgtattg acaactggtc
     9781 gtcccttaaa agctatggat ttctttctcc atgagctagg gactgacggt caggaagatg
     9841 agtataccat tacttttaat ggtggtttgg ttcagaaaaa tacaggtgaa atccttgata
     9901 agacagtctt ttcatatgat gatgtggcac gcttgtatga gaaaacagag aaattatcac
     9961 tgccacttga tgccatctca gaaggaacag tttatcaaat ccaatcggac caagaaagtc
    10021 tttatgccaa attcaatcca gctttgactt ttgttccagt tgactttgaa gacctgtcta
    10081 gtcaaatgac ctacaacaaa tgcgtgactg cctttgctca agaacccttg gatgcagcca
    10141 ttcagaagat ttctccagaa ttgtttgacc aatatgaaat ctttaaatca cgtgaaatgt
    10201 tgctagagtg gtcaccaaag aatgttcata aagcaacagg tttggcaaaa ctaatcagcc
    10261 atctcggaat caaccaaagc caagttatgg cctgtggtga cgaggccaat gacctttcta
    10321 tgattgaatg ggcaggtctt ggtgttgcta tgcaaaacgc tgttcctgaa gtaaaggcag
    10381 ccgcaaatgt agtaatgccg atgaccaacg atgaggaagc tgtcgcctgg gctatcgaag
    10441 aatatgtgct aaaggagaac taagatatgg gattgtttga ccgtctattc ggaaaaaaag
    10501 aagaacctaa aatcgaagaa gttgtaaaag aagctctgga aaatcttgat ttgtctgaag
    10561 atgttgatcc taccttcaca gaagttgagg aagttcctca ggaagaagca gaggttgaaa
    10621 ttgttgaaca agctgtgttc caagaggagg aaatccaaga cacagttgaa gaaagtcagg
    10681 attcagagcc agctgtaggg gtttctcaag aagaaataga agaattacca aactcagaag
    10741 aattaccaga ggaagagaag cttgagcacg aaggaactgt agaagaaaat aattctgaag
    10801 ttcttgaacc agaaaggcct caaacagaag aaactgttca ggaaaaatat gaccgcagtc
    10861 tcaagaagac tcgtacgggc ttcggtgctc gcttgaacgc cttctttgct aacttccgct
    10921 ctgttgacga agaatttttc gaggaactgg aagaactgct gattatgagt gatgttggtg
    10981 tccaagtcgc ttctaactta acggaggaac tacgttacga agccaagctt gaaaatgcca
    11041 agaaacctga tgcacttcgt cgtgtcatca ttgagaaatt ggttgagctt tatgaaaagg
    11101 atggtagcta cgatgaaagc atccacttcc aagataactt gacagttatg ctctttgttg
    11161 gtgtgaatgg tgttgggaaa acaacttcta ttggaaaact agcccaccgc tacaaacgaa
    11221 ctggtaagaa ggtcatgctg gttgcagcag ataccttccg tgcgggtgca gtagctcagc
    11281 tagctgaatg gggccgacga gtagatgttc cagtagtaac tggacctgaa aaagctgatc
    11341 cagccagcgt ggtctttgat ggtatggaac gtgccgtggc tgaaggtatc gatattctca
    11401 tgattgatac tgctggtcgt ctgcaaaata aggataacct tatggctgag ttggaaaaga
    11461 ttggtcgtat tatcaaacgt gttgtgccag aagcaccaca tgaaaccttc ttggcacttg
    11521 atgcatcaac aggtcaaaat gccctagtac aggccaaaga attttcgaaa atcacacctt
    11581 taacgggaat tgttttgact aagattgatg gaactgctcg aggaggtgtg gttctagcca
    11641 ttcgtgaaga actcaatatt cctgtaaaat tgattggttt tggtgaaaaa atcgatgata
    11701 ttggagagtt taactcagaa aactttatga aaggtctctt ggaaggctta atctaatcag
    11761 aagcaaaaat cctgcaaggt acaaacttgc aggatttttt tattcgaaac gaccatcttg
    11821 acgataggcg atatctggtt gccaagtcca tttggcatcg aatttttcaa gtaggtcaaa
    11881 gctggcttga ggtcccatgc ttccagcttt atagtcatga agtggggcac cattttcggc
    11941 ccagagctct tcaatacggt caatcaactt ccatgacgca ccaacctcat cccagtggct
    12001 aaagttagtt gagttgttat ttaggacatc ataaatcaat ttctcgtatg gttctggaga
    12061 agcaccagtt gcagtcgcgt ctgtacgata atcaagtgag ttaggagcca agttaaattc
    12121 ttctcctact tgcttcccat ttaggctaag agagaagcct tctgttggtt gaatatagat
    12181 ggtcaaaata tttggagcaa gtggttctcc aaagatagaa tccatttgtt taaagacgat
    12241 gttgacatga gttccttttt cagtcagtcg tttacctgta cggaaaaaga aaggaacacc
    12301 acggaatcga tcgctgtcta caaagaaggc accagatgta aaggtttcag ttgttgattc
    12361 tggattcaca tttggttcgc tacgataaga gatgtatttc atgccatcaa tcttaccaga
    12421 gcggtattgt ccacggataa agtgttcttt gagttcttca tcagttggat gatagaggtt
    12481 tttaaagacc ttaatctttt cagcacgaat ctcgtctttt gtgaagcttg ctggtttgtc
    12541 catggcgagg agcgaaagaa gttgtagagt gtggttttgg accatgtcac ggagggcacc
    12601 ggattggtca tagtagccac cacgttcttc tacacccaag cgctccgcaa aggtaatttg
    12661 aacattgtcg ataaaatcct tgttccaaac gttttcaaaa atcaagtttg caaagcgaac
    12721 tgcaaagatg ctttggatca tttccttgcc aagataatgg tcgatacgga aaatttgttc
    12781 ttcgtcaaat gttgctagga gttcgtcatt caacttgctt gcagttgcgt aatctgtacc
    12841 aaatggtttt tcaacgatca agcgctcaaa acctttgcca tcgacaatgt tttcagattt
    12901 gaggtgtttg gcaatggttc caaagaactg aggtgccata gacaagaaga agagcttatt
    12961 gtgttcagct tggtattttt cattaagctc agcttgtaat tgacgcaaag caatataatg
    13021 ttccgaatca ttgacatcat ggctttgata gtagaagtgg ctagcaaatt cttgggcttg
    13081 ctcggtacta tctgccaaat caaggatgga ctcgacaact acagattcaa aatattcctt
    13141 actccaaggt ctacgggcag ttccaataac ggcaaagtgc ttggaaagat tgccggattt
    13201 atatagtcta aaaagggaag ggtagagctt gcgtttagcc aggtctccac tcgcaccgaa
    13261 aattgtaaca ataaccttag atgacatcta gctacctaat ttctattttt attcctagtc
    13321 ttgctaggta tgaggaaacc tcatttcata aacttaattc taacataatt ttccgaaaaa
    13381 tcaaaaattc caatacgagg tagaaaaact gcaaggaaat ccttacagtt tgagtttaga
    13441 cgtttaagac cttatctaag aactctttca gacgagggtg ttgtgggtta tcaaagattt
    13501 ggtcaggtgt tccgtcttca aggaactcgc catctgcagt aaagataacg cggttggcaa
    13561 cctgacgagc aaatcccatc tcatgggtta cgataatcat ggtcatgcct tgctcagcca
    13621 attccttcat aacgttaagt acgtctccaa ccatctcagg gtcaagggca gaagttggtt
    13681 catcgaagag catgatgtct ggattcattg ctaggccacg agcgatggcc acacgttgtt
    13741 tttgaccacc tgataggcta tctggattgg cattagcttt atctgctagt ccaacctttt
    13801 caagcaactc cattcccaat tcctcagctt cttccttagt catcaacttg tgctcaatag
    13861 gagcaaaggt gatgttgtcc aatacagaca tatgagggaa gaggttgaag tgttggaata
    13921 ccatgccgat attttcacgg acgtggtcaa cattggttgt tttttcagtt aaatcatagc
    13981 cgttcacagt gatgtgaccg ctagtgactt cttctaaaag attgaggcta cggaggaaag
    14041 ttgacttacc agaacctgaa ggaccgatga tacaaacaac atctccttca tagaacttag
    14101 tcgtaattcc ttttaggact tcattttttc catagtgctt gtgtaaatca tttacatcaa
    14161 tttttaattt tgccattaac gaatcctctt ttctaagcgt ttcgctagtc tagtcaaaag
    14221 tgtgataatt acaagataga agatagcaag gattgcatac atcttgaaac tttggtagtt
    14281 acgagcaatg ataatcttac cagtttggaa gagttcaacc aaaccgatag cagatacgat
    14341 agttgtatct ttaagagcga taacgaattg gttgacaaag tttggcaaca tcaatttagt
    14401 tgcttgtggc aagataatct tacgcatggt ttttccataa gagataccca agcttcggct
    14461 ggcttccatt tggccaactg gaacggcctg aataccacca cgaacgattt cagcgatata
    14521 agcagccgca ttgagtgaga gggcaattgt tccagctaca aagtcgttaa ttgggctttg
    14581 ttggcctgtg atagactcga tgaagtttgg aattccccag aagatgaagg ctgcaagaat
    14641 catcaatgga ataccacgaa taacgtcaac gaaaatctca gagatgacgc gaagagattt
    14701 gtatgggcta acgctaaaca taccgaagat aattccgatg acaatggcaa tagcaaatga
    14761 gataagagct agagcaagag tgataccaag accgctaagg agttgtttgt aattgttttg
    14821 aagcaagccc cagagcgttg tttcgtcaac agtacttgtt gaagcagttg aagattcgct
    14881 agctaggtat ttgtcaagaa tcttttggaa ttcaccgttt gctttaaggt ttgcaagtcc
    14941 gttgttgaac atttcaatca gttctggatt tgctcctttt ttaacggcaa aggctgtttc
    15001 accgattgga gttccagaga ttggagtttt caatttttga ccttggctga tagaatattt
    15061 gagaacaggt tcatcatcca taacggcatc aatggcacca gtatttaaac tgtcatacat
    15121 tgaagaacca tcagcaaagg ttttgatttt gtagccgtat ttgctttgat tttctgttag
    15181 gaaggtttga gaagcagttc cgtttttaac accgactgtc tttcctttta gatcttcata
    15241 agaagcaata ttgcttgatt ctttgacacc aagaatggta ttagcagtgt agtatgattc
    15301 tgagaagtca aaagttgcct tacgagcatc tgtgacagac ataccagcga tgataccatc
    15361 ggcttgacca gcttggacag cactgatagc agcatcaaaa ccagggttgg tgatttcaat
    15421 ttcaaaacct tggtctttag cgattgcctt aatcaattcc atatcaatac cagtgtattg
    15481 gttgcttgaa ttttggaaaa caaaaggtgc aaaagaagaa tcgctggcaa tgatatattt
    15541 agccttaaca ggaatggctt ttaatcctgc gagagtagtt gtagttggca ctgctgaaga
    15601 tgatgaagca gtccatttct tgataatttt atcaagacta ccatcttttt tcatttcaga
    15661 caaggcttgg ttaaattcag taaccaggtg ctcgtattta cttccttttt tcacaccgaa
    15721 agcaaaactt cctacagctt caccatccat ttcaatatgg aggtcttgac cttggttaat
    15781 ggcatattcg ataacaggtt tgtcatccat catggcatcg atggcaccag cactcaagct
    15841 gttgttcatt aaatcaccag tgtcaaatgt tttaatagta aagccgtatt tatctttgat
    15901 tgtttcaagg aaacgttgag cggcagttcc gtttttaaca ccaacggttt tgccagttaa
    15961 ttggtcgtac ttgctaattt tgtgtgactt tgtagtagca atgacaactt ttgtatcata
    16021 gtaagtatca gacatggtga agactttttc acgttcttta gtctttgtca tccctgccat
    16081 gatagcgtcg gcttgcccag cttgaaccgc attgactgct gcgtcaaatc caggatagga
    16141 catctgaatg ttccagcctt taatctcagc gactttgtta ataatgtcaa catcaattcc
    16201 tttataagtt tgatctgaat ctttaaactc aaaaggtgca taggcggtat cagaaacaat
    16261 cttaatcgtt tctgctttgg caatacctaa tgagaaaatt gggaataaaa ttagcaaaaa
    16321 tgctagaaat tttttcttca tttttagtct ccttttccga atattctctc attatatcag
    16381 aaaaagtaaa aaaaggcaaa tttttatatt tttcgtgtca gaaaatataa catcgatttt
    16441 ttcctttcct tgaattgcta ttaaaaagtg ctataataat agtatatata gaagaaaaga
    16501 ggacggggat atgaagaaca aaagaatatt taaagacttc caagcttcaa aaatgagttt
    16561 aaacatttac acaagcccct tgttagcctt tgtttttgtc ttcataggag agtttgtggc
    16621 ttttactttg tatggtattg gcttgttagc tctcatcgga cttgctagaa attttggaga
    16681 ggctggtcaa aatcttgcaa gctacttgca gaccttgcat cagagcttga cggataaaac
    16741 aagtgacttt cgtttaattt taggattact ggcctttggt tttattctta acactgtgtt
    16801 cagatggaca agaaaagttg agaaaagacc tattcgaacc ttgggatttt atagagagaa
    16861 tttcctcagc aatcttctga aaggatttag tctaggtctg gcactttttc ttctgacctt
    16921 gttaggtttg gtggtcttag gtcaatatcg tttggaatcc attcacttga atccttattc
    16981 tcttgccttt gtcgtcttta ctatcccatt ttggatttta caggggacag cagaagaagt
    17041 ggtggcccgt gcttggctac ttcctcaatt ggcctcaaga accaatctaa aactagctat
    17101 tcttatatct agcctgttct ttaccctgct tcatatgggc aattctggcc tcacccctct
    17161 atctctagta aatctctttt tattcggagt tgccatggct ctttaccttc tcaaaactga
    17221 tacagtttgg ggtgttgcag gtattcatgg tgcttggaat tttgctcagg gtaatctctt
    17281 tgggatttta gttagtggtc aaccgtcagg aacgtctctg atgacctttt taccacaagg
    17341 caatcaagat tggctatcag gtggttcttt tggcatagaa ggttccatta tgacaagtct
    17401 ggtattacta ctgctgattg tctatcttgc caataaatta aagaaagaaa atgaaaggat
    17461 gtgacttcgg tccgtccttt tcttcgtgaa aatactataa gtatgctaaa ataggaatag
    17521 cacatggaga gaggattctt atgatcaatc acattacaga taatcaattt aaactagtat
    17581 caaaatatca accatcagga gatcaacccc aagctatcga gcagttggtg gataacattg
    17641 aggggggaga aaaagctcag attctgatgg gggcgactgg aacagggaag acctatacta
    17701 tgagtcaggt catttctaaa gtcaataaac caactctggt tattgcccac aataaaactc
    17761 tggctggtca gctctatggg gagtttaagg aatttttccc tgaaaatgca gttgagtatt
    17821 tcgtatccta ctatgattat taccagccag aggcctatgt cccttctagc gatacctata
    17881 ttgagaagga tagttctgtc aatgacgaga ttgacaagct tcgccactca gctacctcag
    17941 cccttttgga gcgtaatgat gttattgtcg tggcctcagt ctcttgtatc tatggtttgg
    18001 gttcgcccaa ggaatacgct gatagtgtcg ttagtctccg tcctggtcta gagatttctc
    18061 gtgataaact cttgaatgac ttggtcgata ttcagtttga acgtaatgat attgatttcc
    18121 aacgcggaag atttcgcgtt cgtggggatg tggtagagat tttcccagct tcccgagatg
    18181 aacatgcctt tcgagtagaa ttttttggag acgaaattga ccgtatccgt gaggttgagg
    18241 ctctgacagg tcaggtattg ggagaggtgg atcatttggc gattttccca gctactcact
    18301 ttgtgaccaa tgacgaccat atggaagttg ccgttgccaa gattcaagca gaattagaag
    18361 agcagttagc tgtctttgag aaggaaggta aattgcttga agcccagcgc ttgaaacaac
    18421 ggacagagta cgatatcgaa atgctgcgcg agatgggcta tacaaatggg gttgaaaatt
    18481 attctcgcca catggatgga cggagcgaag gagagcctcc ttatacgctt ctcgactttt
    18541 tcccagatga ttttttgatt atgattgacg agagtcatat gactatggga caaataaaag
    18601 gcatgtacaa tggagaccgt tcgcgtaagg aaatgctagt aaattatggt ttccgtttac
    18661 cgtctgcctt ggacaatcgt cctctccgtc gtgaggagtt tgaaagccat gtgcatcaga
    18721 ttgtttacgt atcagcgaca cctggtgact atgaaaatga acagactgag acggtgattg
    18781 agcaaatcat tcgaccgaca gggcttttgg atcctgaggt ggaagtccgt ccgactatgg
    18841 gacagattga tgatctctta ggtgaaatca atgcccgtgt tgaaaagaat gaacgaacct
    18901 ttatcaccac cttgaccaag aaaatggcag aggacttgac cgactatttc aaggaaatgg
    18961 gcatcaaggt caagtacatg cactcggata tcaagacctt ggaacggacg gagattatcc
    19021 gtgatctccg cttgggtgtc tttgatgtct tggttggaat caatctcctt cgtgaaggga
    19081 ttgacgttcc tgaggttagc ttggttgcca ttctcgatgc tgacaaggaa ggtttccttc
    19141 gtaacgagcg tggactcatc cagaccattg ggcgtgccgc ccgtaacagt gaaggtcatg
    19201 tcatcatgta tgcagatacg gttacccagt ctatgcaacg tgctatcgat gaaactgccc
    19261 gccgtcggaa aattcagatg gcctataatg aagagcatgg tatcgtacca cagacaatca
    19321 agaaagaaat ccgtgacctg attgccgtga ccaaggcagt tgctaaggaa gaggacaagg
    19381 aagtcgacat caatagcctc aacaaacaag agcgcaaaga actcgtcaag aaactagaaa
    19441 aacaaatgca agaagccgtc gaagtgcttg actttgaatt ggcggctcag atccgtgata
    19501 tgatgttgga agtcaaggcc ttggattagg ggaattagta tgatttattt aagaaagtta
    19561 aagaaagaag atttgatgtc tttatgggaa atggcttatt cacagcttaa tccagtttgg
    19621 aaacagtatg atgctcccta ttatgatgat tatcagtatt tttcaaattt taaagaattc
    19681 gaactacaaa aatcagaatc cattttaagc aactcaaatc gccttggtat ttttgttgat
    19741 gataaactag ttgggactgt ttcgcgttat tgggtatgta aagaaacaag atggatggaa
    19801 ttgggaattg gtatttatga taaaaaattc tggaacactg gtattgggaa agttgctatg
    19861 ttgcagtgga tagataggac gtttcaggat tacttggagt tggagcatct ggttttgaca
    19921 acttggtcag gaaatcttgg tatgatgaaa cttgctgaaa aattaagaat gaaaaaagaa
    19981 gctcatattc caaaagttcg ttattatcaa ggtaaatatt ttgatagtat taaatatggt
    20041 attttgagag aagactggga gaaaataaat gacggttatt atcaaatcaa tggaaactcc
    20101 tgaagagata gaaggcaaat ccctcgttca ctggcaaacg tggagagagt cttatgatga
    20161 ccttttgcct gcggaatttc aggatacaat gacattagaa agatgtcgat tctttagtca
    20221 aaagtatcca gaaaatacat tgattgcgat ggatggcgtg aaggtggttg gttttatcag
    20281 ttatggcaat tttcgtgatg agactattca agctggtgaa attattgctt tatatgtttt
    20341 aaaagactat tatggaaaag gtatcgcaca aaagttaatg aaagcagctt tgactgctct
    20401 taatcatttt tctgaaattt tcttatgggt attgaaagat aacaagcgcg ccattgcttt
    20461 ctatcaaaaa atgggtttta cttttgatgg acaagaaaaa atacttgaac ttggaaagcc
    20521 tataaaggaa aaacgaatgg tgttctattc taaataattt taaaaaagta aaagataata
    20581 tctctggtag taagtctgaa aatttaataa attagaaagc gagtaaattt atgtcccgtt
    20641 cccaattaac aattttaaca aatatctgtc tgattgaaga cctcgaaact cagcgcgtgg
    20701 tgatgcagta tcgcgcccct gaaaacaatc gctggtctgg ttatgccttt cctggaggtc
    20761 atgtagaaaa tgatgaggct tttgcggagt ctgtcattcg tgaaatctac gaagaaacag
    20821 ggttgactat ccaaaatcct caacttgtcg gcattaaaaa ttggccacta gatacaggtg
    20881 ggcgctatat tgtcatttgt tataaggcga ctgagttctc tggtaccctt caatcttcag
    20941 aagagggaga agtttcttgg gtgcaaaaag accagattcc aaacttaaat ctggcctatg
    21001 atatgctacc attgatggaa atgatggaag ctcccgacaa gtcagagttt ttctatcctc
    21061 gccgtacaga agacgattgg gaaaagaaaa tcttctagtc ttttactaaa taacctagct
    21121 gatccaaggc ctcctcgata tagtggaggt cttgttgtgt ttcggcttcg actaggtggt
    21181 aatggatgcc atctgtcaat tcagatagag gtctaaagtc agaatgctcg acttgttcta
    21241 gaaaatgttg cacatctctg cgacaagaca gtttaagcaa ggtttcaatt tctccataaa
    21301 cgggatggtc aatcaaggta ttctggacat gcccaccatt atcgacaata gcaaggagtt
    21361 cctgaccgat ttcttcaact tcatgtttca ctttgaaaag tttgtgaaca taaggattgg
    21421 tatcaatttg cttatagatg tagccacgat tggtggatag gataggagcg ccatcggctc
    21481 tcaaaattgc aatgtcctgc acaatgacct gacgtgtgac atgaaaatgt tccgccaaac
    21541 tttggccatt aagggcttta ggagcttctt tcaagagttg gagcagggct tgtttgcgat
    21601 cttttgtcat agcttttcct tttaacggcg ttttcgaagc actttataga cagctagtgc
    21661 taatgtatag tctaccatac tatggataat tgtgccaaat ccaactagta caaatagaac
    21721 atagaacata ttttctacat tggtacccga agttgcgtaa aaaaggacac aggctaatac
    21781 ttcagcaagg gcatgaacaa cagccaaaac aaaattgaaa acccaggaag attttggttt
    21841 atctagggta tcggggaatt tttgtaggta aagagctcct aaagcaccaa aagatatatg
    21901 ggaaaaagcc cgaaaaacga taaccatggg atagccagcc atcaaaaatc caaaactaga
    21961 ggctaggatg acaaaaactg ccatcaaggg cgacaagaac atggctataa aaatagcgat
    22021 gtggctcccc aaagtatagg aagcaggtgg aatgacaatc ttgaaaggca taacaattgg
    22081 aatcaaaatc gcaatagccg ttaaaagggc tgtcattgtc ataaattgtg tctttttccg
    22141 tgtattcaca agaatctcct ttttaactgc atatacacta gtatggtaca ataaaccaga
    22201 caataaagca agaatttact tgggtttata gatcattttt tagttaaaag ttatagtaga
    22261 tggaaactag aatagtccac ctctacttct aaaacattgt tagaaatcga tttgactgtc
    22321 ctgatcgatt tgtcctgttc ttatttcgtt ttactatagt aaagatttca ttaaaaagaa
    22381 actgtataga gcaaaatctc caccttcagg tttgaaagcg gagattgttt ttattttttc
    22441 aaggtttgta gtcgtggaac aatggctagg gttagaattg cagaaatgac caattcagca
    22501 attgaatttg ttgagataac ggttgccaga agaagttgga tatttccatt ataaacattt
    22561 ccaaatagga agaagattcc tccaaggaca aagatagtat ttgtcaagga accgagggct
    22621 ccagctaaaa tcagaccagt cttgtttttc atcagtttat agactaagta aggagttaaa
    22681 ccaatcaaaa tacgtgggac gatggcaatg atagctgagt agatgtttcc gtttggtacg
    22741 aagggagaga agaggtagct tgtcggtaga atcgtaatcg tgttaaccgt caagctaagt
    22801 aatcccatca aaaatccaag tgtaacccca actcgtggac cataaataat gctggcaata
    22861 atgacaggaa tatgaacaat ggtcggtttg attggaaatg gaaaaaggtt aaagataagt
    22921 gagctcagaa agtgtatcac gagcatggta gcaaaaaaga tagcaatggg tgcaatatta
    22981 gagcgttttt tcatcgatag tttcctttat tctttctaaa ataattgtga ggtcagctaa
    23041 agctcctcgt ccgtggtctc cacaagctag tagggattcc ttaggagcaa tcagctgata
    23101 gccgtaggtt tctaatgttt tcagattatt ctgagttact ggatggtcat acatttttgt
    23161 attcatagca ggagctatta gtttgggaat atgacttggt agggctagag ctgtactggt
    23221 taccatgttg tccgcaaatc cgtgagctag ttttgcaata gtgttagcag ttgcaggtac
    23281 cacgataaat aaatctgctt tttttccaag ttcgatatga ttgacctgat caggataggg
    23341 ttccttcatg acatccaagt ggacaggatt ctgtgagagt acctgtagtg tcaaaggttg
    23401 gataaactct gtagcagcct gagtcattaa gacagtgact tgatggcctt gtttttttag
    23461 agaactgact aaatctgccg acttataaga ggcgattgaa cccgttacag ccaagagaat
    23521 gtttgccata gttttccttt ctaagaatga taggcttgaa ttttttcaag gaggagttct
    23581 gcaatttctt ctttagtctg gactgtttga agctgatttt tctcaacaaa tatagctcgg
    23641 tgctgatctg ctgaaatttg agtcaggtca ttcgcgatga ttaaatctgc ttgattcttg
    23701 ataagacttt ttcgtgcaat gtcaaccaga tgatcttcgg taacatcaac cagcagtttg
    23761 aaaccaatca gatgaatagt gggattccat tccttgacta gggatatgat tttgggtgtc
    23821 tttttaagga acaaaacctg aacctcatca gttgaagaaa tcttggcctg atgattctgc
    23881 ttgcttaaaa attcttttag attggagcta gcctgaactt cctcaagccc tgtcatataa
    23941 acaggagtgt agtcagaaac agccattgag tggatcaaga cctgataatc ctgaacacgt
    24001 tcttgcattt ctattagaag gttcttggta ttggtaattt ctcgaatact taggttagga
    24061 tgaggctctg gcttcagagc tcgttttgtc gtaattaaac aaacttcatg ccctgcagaa
    24121 agcaaagtct ctgtgataat tttccccaag tgacctgtag aatggttagt gatagagcgg
    24181 acgctatcga tagcttcact ggtaccgccc gatgtaacta aaattttcat agcactattg
    24241 tacacaaaaa taagcggtaa gtaaaatgaa agtgataaat ttttggtaaa aacgaagatt
    24301 tactatagtt tcaaactata aagccatata aaatttaaga aaggactcta aaatccttaa
    24361 aaacagagat atttacgaac gtttagagag ttttaggatg ttaaaaatct tgttttgtga
    24421 tataatgaat tatcatataa gaggttagag gaattttgaa tgaaaacaga tattgaaatc
    24481 gcacagagta ttgagttgaa gccaattgtt gatgttgtag agaaacttgg tatttcttat
    24541 gacgatttgg agttgtacgg aaagtacaag gctaaactca gctttgataa aattcgtgca
    24601 gttgagagca atccagtcgg taaattgatc ttggttactg ccatcaaccc aacacctgca
    24661 ggtgaaggaa agtcgacgct taccattggt cttgcggatg ccttgaacaa gattggcaag
    24721 aaaaccatga ttgctatccg cgaaccgtct cttggtccag tcatggggat caagggtggt
    24781 gctgctggtg gtgggtatgc acaagttctg ccaatggaag acatcaacct ccactttact
    24841 ggagatatgc atgctattac aactgccaac aatgcccttt ctgccttgat tgacaaccac
    24901 ttgcaccaag ggaatgagct ggaaattgat caacgtcgta tcctctggaa acgcgttgtg
    24961 gacttgaacg accgtgcgct ccgccatgtg actgttggtc ttggtggtcc tctaaacggt
    25021 attccacgtg aggatggttt tgatattaca gttgcttcag aaatcatggc aattctttgc
    25081 ttggcaacgg acatcgagga cttgaaacgt cgtttggcga atatcgttat tggttatcgc
    25141 tatgaccgta cgcctgtttc tgtaggtgat ttgcaggttg agggtgcctt agctttgatt
    25201 ttgaaggatg ctattaagcc aaacttggtt cagacaattt acggtacacc tgcctttgta
    25261 cacggtggtc catttgccaa tatcgctcat ggctgtaact ctgttttggc gacgacaaca
    25321 gcccttcact tggctgatta cactgttact gaagctggtt ttggtgcgga ccttggtgct
    25381 gagaaattcc ttgatatcaa gacaccaaac ttgccaacat ctccagatgc agttgtcatt
    25441 gtcgcaaccc ttcgtgccct taagatgaat ggtggtgtgg ctaaagacgc tctgactgaa
    25501 gaaaatgtag aggcagttcg tgcaggtttt gctaacttga aacgccacgt tgaaaatatc
    25561 cgtaagttcg gtattccagc agttgtagct attaacgaat ttgtatctga tacagaagct
    25621 gaaattgcag tcttaaaaga actctgtgcc tcaatcgatg taccagttga attggctagt
    25681 gtctgggctg atggagcaga aggtggagta gcacttgccg aaacagttgt taagacaatt
    25741 gctgaaaatc cagctaacta taaacgtttg tacgataatg ccctttctgt ccaagaaaag
    25801 attgaaaaaa ttgtcactga aatctatcgt ggtagcaaag tgaactttga gaagaaagct
    25861 caaacacaaa ttgctcaaat cgttcaaaac ggttgggaca aattgccaat ctgtatggct
    25921 aaaactcaat acagtttctc agacaatcca aatgcgcttg gagcaccaga aaactttgaa
    25981 attaccattc gtgaattggt accaaaatta ggtgcaggct tcatcgttgc cttaactggt
    26041 gatgttatga ccatgccagg tcttccaaaa cgtccagcag ctctcaacat ggatgttgaa
    26101 agcgatggaa ctgtactagg cttgttctag tggattgaaa ttgactttaa gtgagtttgg
    26161 aaataatatc caagctcatt ttttctcaga ctgcaagcag ttcatcaata agatttatac
    26221 tcttcgaaaa tctcttcaaa ctacgtcagc ttcaccttgc cgtaggtata tgatactgac
    26281 tctgtcagtt ctatccaca
//
LOCUS       DAVKLW010000027        24940 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKLW010000027 DAVKLW010000000
VERSION     DAVKLW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24940
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.027"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            95..583
                     /locus_tag="VW324_001118"
     CDS             95..583
                     /locus_tag="VW324_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726767.1"
                     /translation="MGTKQRLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNL
                     ALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRD
                     IFITKQEFGDIVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNR
                     SK"
     gene            599..1327
                     /locus_tag="VW324_001119"
     CDS             599..1327
                     /locus_tag="VW324_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726768.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLGLLISFIPPILVTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTIMMNTSNDLFIKAIELLPFYSLPVFMASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            1706..1915
                     /locus_tag="VW324_001120"
     CDS             1706..1915
                     /locus_tag="VW324_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9726769.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1905..2393
                     /locus_tag="VW324_001121"
     CDS             1905..2393
                     /locus_tag="VW324_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726770.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            2509..3969
                     /locus_tag="VW324_001122"
     CDS             2509..3969
                     /locus_tag="VW324_001122"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9726771.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3966..4790
                     /gene="nadE"
                     /locus_tag="VW324_001123"
     CDS             3966..4790
                     /gene="nadE"
                     /locus_tag="VW324_001123"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9726772.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5140..5691
                     /locus_tag="VW324_001124"
     CDS             5140..5691
                     /locus_tag="VW324_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726773.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKVYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            6406..7098
                     /locus_tag="VW324_001125"
     CDS             6406..7098
                     /locus_tag="VW324_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9726774.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            7152..7541
                     /locus_tag="VW324_001126"
     CDS             7152..7541
                     /locus_tag="VW324_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9726775.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(8202..9230)
                     /gene="queA"
                     /locus_tag="VW324_001127"
     CDS             complement(8202..9230)
                     /gene="queA"
                     /locus_tag="VW324_001127"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9726776.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDKWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            9381..10088
                     /locus_tag="VW324_001128"
     CDS             9381..10088
                     /locus_tag="VW324_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9726777.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            10228..10404
                     /locus_tag="VW324_001129"
     CDS             10228..10404
                     /locus_tag="VW324_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9726778.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            10534..11469
                     /locus_tag="VW324_001130"
     CDS             10534..11469
                     /locus_tag="VW324_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9726779.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            11462..12250
                     /locus_tag="VW324_001131"
     CDS             11462..12250
                     /locus_tag="VW324_001131"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9726780.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGAIVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            12251..12634
                     /locus_tag="VW324_001132"
     CDS             12251..12634
                     /locus_tag="VW324_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9726781.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            12650..13039
                     /locus_tag="VW324_001133"
     CDS             12650..13039
                     /locus_tag="VW324_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9726782.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            13123..14253
                     /locus_tag="VW324_001134"
     CDS             13123..14253
                     /locus_tag="VW324_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9726783.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            14258..14995
                     /locus_tag="VW324_001135"
     CDS             14258..14995
                     /locus_tag="VW324_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9726784.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            15006..15779
                     /locus_tag="VW324_001136"
     CDS             15006..15779
                     /locus_tag="VW324_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9726785.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            15769..16386
                     /locus_tag="VW324_001137"
     CDS             15769..16386
                     /locus_tag="VW324_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9726786.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            16596..16997
                     /locus_tag="VW324_001138"
     CDS             16596..16997
                     /locus_tag="VW324_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9726787.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            16997..17275
                     /locus_tag="VW324_001139"
     CDS             16997..17275
                     /locus_tag="VW324_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9726788.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            17265..18038
                     /locus_tag="VW324_001140"
     CDS             17265..18038
                     /locus_tag="VW324_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9726789.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            18039..19343
                     /locus_tag="VW324_001141"
     CDS             18039..19343
                     /locus_tag="VW324_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9726790.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            19917..20795
                     /gene="pstS"
                     /locus_tag="VW324_001142"
     CDS             19917..20795
                     /gene="pstS"
                     /locus_tag="VW324_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9726791.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            20795..21712
                     /gene="pstC"
                     /locus_tag="VW324_001143"
     CDS             20795..21712
                     /gene="pstC"
                     /locus_tag="VW324_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9726792.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            21702..22586
                     /gene="pstA"
                     /locus_tag="VW324_001144"
     CDS             21702..22586
                     /gene="pstA"
                     /locus_tag="VW324_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9726793.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGIYLHKKLTAA"
     gene            22597..23400
                     /gene="pstB"
                     /locus_tag="VW324_001145"
     CDS             22597..23400
                     /gene="pstB"
                     /locus_tag="VW324_001145"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9726794.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            23413..24171
                     /gene="pstB"
                     /locus_tag="VW324_001146"
     CDS             23413..24171
                     /gene="pstB"
                     /locus_tag="VW324_001146"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9726795.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            24183..24836
                     /gene="phoU"
                     /locus_tag="VW324_001147"
     CDS             24183..24836
                     /gene="phoU"
                     /locus_tag="VW324_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9726796.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
BASE COUNT         7350 a         4478 c         5646 g         7466 t
ORIGIN      
        1 atatggtgtt gactacaatc aagaaagatt tagaagtttg attcaagagt tagatttgac
       61 tcagtctatt aataaaaaag taaagaccta ttctttgggt acaaaacaaa gattagcttt
      121 gcttctaact ctcgttacgg aacctgatat attgatttta gatgaaccga ctaatggttt
      181 agatattgaa tcatcacaaa tagttttatc ggttctaaaa aatttagctt tacatgaaaa
      241 tgtgggaatt ttaatatcga gtcataaatt agaagatatt gaagaaattt gtgagagggt
      301 tcttttcttg gagaacgggc ttttgacatt tcaaaaagta ggaaaagata gtcataattt
      361 cttgtttgag atagcttttt catcagctac agatagagac attttcatta ccaaacaaga
      421 atttggggat attgttcagg aagagggact gagaattact atgtctggga atattcaaag
      481 tagtgagctt tttaaatttt ttaacgaaaa ctctattaaa gtagttgatt ttgaaactaa
      541 aaaagagacg cttaaagata tttatctaaa tcgttcaaaa taaaggaagg ttataatcat
      601 gaaattaaat aaattgaatt ttcttaagga aaatataaga aatttatatt catcaggcgt
      661 aatatatctc ggtttgctta tctcgtttat accgccgata ttggttacat tctttattct
      721 aaagactcaa gggacatcgc ttggtattaa gcatatttca aacttttatg ctatgctcgg
      781 tatgttaatg gctgttatac atgctaatcg agtcattagt agagattttt cccacaatac
      841 ggtaagtttg ttttataatc aacagaagaa tcggatgatt tacgtcttgt ctaattttct
      901 atatgccatc tcagtttcca ttatttatgc tttgaatggc attgtgttac tagtcatagt
      961 aagtaaattg ggtattccag gtgatttagg attagatttt atagtagcta ttgtagtcaa
     1021 tacaattttg ttagtcctgt tttattttct attatcttac attttctatt tatacaaatt
     1081 gaaaagtggc ttggtatttg gtattttagt agctttacta ctctttatcc ctaatatatt
     1141 aaatacgatt atgatgaata ctagtaatga tttgtttatc aaagcaattg aacttcttcc
     1201 tttttattct ttacctgtat ttatggcttc aaatacgatg tctattagtc agtatcttgt
     1261 ggtaatcact acaatcattt tattgtactt tttcactctc aagaaaagca agaagtattc
     1321 attttagttt catttagtat tattttgcat acctaaaata cagtaaaaaa tcggtcatct
     1381 tggtatgctc ctgctcttgt tgtgtacacg tttttgtctt atgctagact catttccaaa
     1441 agcattatat aatagtgata tgaaatcaac taaataagaa atacaagcaa tcaaaactcg
     1501 tttaaaagac cctactaaag ctaatactaa ataaaaataa aagagtaaac taggaagttt
     1561 atttcaaaca acctaaaata ctgattttcg gctgaagata atactggagt gcaaattaat
     1621 ggggttataa taaatagctg atagcttgtg ttggttttgg attttttaag agtagatgag
     1681 tattaaaact ataaggagga cgaaggtggc taaaaattta aaattaaaat tagctcgggt
     1741 agagcgtgat ttaacacaag gtcaactggc agaggctgtc ggggtgacac gccagactat
     1801 tggtttgata gaggcgggaa aatacaatcc cagtctctcg ctctgccagt ctatttgcag
     1861 atgtttaggg aaaaccctag accaactatt ttgggaggaa gaagatgaaa aatagatttt
     1921 attattatca attactagac gaaagagaag aacaactgtt caataaagcg ggctctgaaa
     1981 gtttctatat ctgcattgct ttgtcgctcc tatcttatat catttcagta ttagcaccaa
     2041 gcctttttaa ttctaatatg ctgctaatcg ttatcatcat agggacattt tactttttca
     2101 atcgtgcccg ttatctggga gtgacctact atagtcgttt tcattttacg attttgggtt
     2161 gttttttcct aaccttggct attacggctc ttttgatgtt gcagaattat caattcaaca
     2221 tagaaattta tcagcacaat cctttgaatt ttaaatacct gtctgcttgg gtcattactt
     2281 atgtcattta ccttccgtgg gtctttattg gcaatcttgg tcttaagagc tatggcgaat
     2341 gggctcagaa aaaatttgaa caagatatgg atgaattgga gagtggagaa tagcttgtta
     2401 ctcttttctc aatccagcta aaatgtgata taatagtact aatttattgg aatacatgaa
     2461 agttcttgaa aattttcatg ggtttctagc taaggaagta ggaaaagtat gtatccagat
     2521 gatagtttga cattgcacac ggacttgtac cagatcaaca tgatgcaggt ttactttgac
     2581 caagggattc acaataagaa ggcggtcttt gaggtgtatt tccgccaaca gccttttaag
     2641 aacggctatg cggtttttgc aggtttggaa agaattgtga actatcttga agacttgcgt
     2701 ttttcagata gtgatatagc ctatttggag tcgcttggtt atcatggggc gttcttggat
     2761 taccttcgca atttcaagtt ggagttgacc gttcgttctg cccaagaagg ggatttggtt
     2821 tttgctaatg aaccgattgt gcaggtggaa ggacctctag cccaatgtca gttggtcgaa
     2881 acggctcttt tgaacatcgt caactaccag accttggtgg cgacgaaggc agctcgtatt
     2941 cgttcggtta tcgaagatga acccttgatg gagtttggga cacgtcgggc tcaagaaatg
     3001 gatgcggcca tctggggaac acgcgcagcg gtgattggtg gcgccaatgg aaccagcaac
     3061 gtgcgtgcgg gtaagctctt tgacattcct gttttgggaa cccatgccca tgccttggta
     3121 caggtttatg gaaatgacta tgaagctttc aaggcttacg ctgcgaccca caaaaattgt
     3181 gtctttcttg tggataccta tgacaccctt cgcatcggtg taccagctgc cattcaggtg
     3241 gcgcgtgagc tgggtgatca gattaacttt atgggtgtgc ggattgactc tggggatatt
     3301 gcctacattt ctaagaaagt ccgtcagcaa ctggacgagg ctggatttac agaggctaag
     3361 atttatgctt ctaatgattt ggacgaaaat actatcctca atctcaagat gcaaaaggcc
     3421 aagattgatg tctggggcgt gggtaccaag ctgattacag cttatgacca gccagctctt
     3481 ggtgcagttt acaagattgt tgcaatcgaa gatgaaactg gtcagatgcg caatacgatt
     3541 aagctgtcta ataatgctga aaaagtgtcg acgccaggta agaagcaggt gtggcgcatt
     3601 accagtcgtg aaaaaggcaa gtcagaaggc gactatatca cttatgatgg tgtggatatt
     3661 agcgacatga cagaaatcaa gatgttccat ccgacctata catacatcaa gaagacggtt
     3721 cgtaattttg atgccgttcc tctcttggtg gatatcttca aagaaggaat attagtttac
     3781 aacttgccta gtttgactga cattcaggat tatgcccgta aggaatttga caagttgtgg
     3841 gatgagtata agcgtgtgct caatccgcag cactatccag tggatttggc gcgtgatgta
     3901 tggcaagata agatggactt gattgataag atgcgcaagg aagcccttgg tgaaggagaa
     3961 gaagaatgag tttgcaagaa acgattatcc aagagctggg tgtcaaacca gtgattgatg
     4021 cccaggaaga aatccgtcgt tctattgatt tcttaaaaag atatctgaaa aaacatccct
     4081 tcctaaaaac ctttgtacta gggatttctg ggggacaaga ctcaaccttg gcagggcgtt
     4141 tggcgcaatt agctatggaa gaactgcgag ctgaaacggg agacgacagc tacaaattta
     4201 tcgctgtccg cctgccatac ggagtgcaag ctgatgaagc agatgctcaa aaagccctag
     4261 ccttcatcca gccagatgtc agcttggttg tgaatatcaa ggaatcagct gatgccatga
     4321 cagctgcagt tgaagcgaca ggtagtcctg tttcagactt caacaagggg aatatcaagg
     4381 cacgttgccg tatgattgct cagtatgccc ttgctggttc ccatagcgga gcggtcattg
     4441 gaacagacca cgccgcggaa aatatcacag gtttctttac caagtttggt gacggcggtg
     4501 cggatattct ccctctttac cgcctcaata aacgccaagg aaaacagctc ttgcagaaac
     4561 ttggcgcaga gccagccctt tatgaaaaaa tcccaacggc agacctagaa gaagataaac
     4621 caggcctagc tgacgaagtc gcacttggag tcacctacgc agagattgac gactacctag
     4681 aaggcaaaac aatcagccca gaagctcaag cgaccattga aaactggtgg cacaaaggcc
     4741 aacacaaacg ccacttaccc atcaccgtat ttgatgactt ttgggagtaa aaaggtccgg
     4801 gggacctttt tagcttcttg ccctgaaatt aaaaagcaag aaaaacctcc actggaggtt
     4861 ttcagcctct catcttgaaa taagaaagtg agagaaggtc cgggggatct tgaaccccga
     4921 gtttagaaat aagaaaatga ggcagattcg gtaactcgaa gagttcgatt tcatcgtctt
     4981 acccctgcaa cgatgactag gtttgaaaaa gattgctaga gcgcatttca aaccaggcag
     5041 cgactgcgtc aagaaattag aagacaaact cgttttctag ctgttactga gttgagtctt
     5101 tttactacga gcatagaaat aaggaagtga ggtagcatca tgaaatctat cggtacgcaa
     5161 atattacaga cagaacgttt gattttaaga agatttgtgg agagtgatgc agaagctatg
     5221 tttcaaaatt gggcttcatc cgctgagaat ttgacctatg ttacctggga tccccatcct
     5281 gatgtcgaaa tcactcgaaa ctcgattcgc aattgggttg cttcctatac taatctcaac
     5341 tattacaaat gggccatttg tctaaaagaa aacccagagc aagtgatagg agatatcagc
     5401 atcgttaaga tagacgaggc tgatttaagc tgtgaaatcg gctatgtgct aggcaaggtt
     5461 tactggggac atggtatgat gacagaggcc ttgaaagctg tcttggactt ttgttttact
     5521 caagcaggtt ttcaaaaggt cagagcacgt tatgccagtc tcaacccagc ttcaggtcgt
     5581 gtcatggaaa aggctggaat gtcctatcta caaaccattg ttaatggtgt agagagaaaa
     5641 ggctatcttg cggatcttat ttattatggt ataagtaggg aagaatgttg aattctattt
     5701 tctgtttcta tcgaagtcaa ctatttattg taaatataat aattagcatt ccaagtttat
     5761 ttgcaatttt aaaatagcat attgattagt acaagacaga tgttctagtt ccttctttaa
     5821 tctggtttag tgttagttaa aaaatcgctt taagcttgta actaagaggg agctaatcga
     5881 ctagattctc cagccgaaca ggtggtaatg tactttttat agtgtaatcc tagctgttgt
     5941 taaatttaaa atagaatcct ctatcgagtt agggaattaa attcaaccaa ttttattcat
     6001 gttttttcta tcaaattatc taatattaaa atagtctcat tctgatgaga aaactattct
     6061 atttatactg catagttgta taaggaatga tgaaagatga gtgaaaatta tcaagttgga
     6121 atgtttgtat ctaaatatat tagcatgtat ttagataaga tgtctgcaat cctttatata
     6181 tgagattgat tacgatatag ttaaagtgtt agttcaataa tcaatatatc attctaagtc
     6241 tattttatat ataaagtata ttgtttaacg tttgatagaa aagatgctat actagaagta
     6301 ggatgtaatg gcaaaatcta atactaataa gcgctatgca atacgtaaac tcggaaagat
     6361 ttttggttct gcggtaatcg ggctaacttt atttttagga ggaatctgaa tacaagtttt
     6421 gttcatgctg ctgatgggat tcaatatgtc agagatgata ctagagataa agaagaggga
     6481 atagagtatg atgacgctga caatggggat attattgtaa aagtagcgac taaacctaag
     6541 gtagtaacca agaaaatttc aagtacgcga attcgttatg aaaaagatga aacaaaagac
     6601 cgtagtgaaa atcctgttac aattgatgga gaggatggct atgtaactac gacaaggacc
     6661 tacgatgtta atccagagac tggttatgtt accgaacagg ttactgttga tagaaaagaa
     6721 gccacggata cagttatcaa agttccagct aaaagcaagg ttgaagaagt tcttgttcca
     6781 tttgctacta aatatgaagc agacaatgac ctttctgcag gacaggagca agagattact
     6841 ctaggaaaga atgggaaaac agttacaacg ataacttata atgtagatgg aaagagtgga
     6901 caagtaactg agagtacttt aagtcaaaaa aaagactctc aaacaagagt tgttaaaaaa
     6961 agaaccaagc cccaagttct tgtccaagaa attccaatcg aaacagaata tctcgatggc
     7021 ccaactcttg ataaaagtca agaagtagaa gaagtaggag aaattggtaa attactctta
     7081 ctacaatcta tactgtagat gaacgtgatg gaacaattga agaaactact tctcgtcaaa
     7141 ttactaaaga gatggtaaaa agacgtataa ggagagggac gagagaacct gaaaaagttg
     7201 ttgttcctga gcaatcatct attccttcgt atcctgtatc tgttacatct aaccaaggaa
     7261 cagatgtagc agtagaacca gctaaagcag ttgctccaac aacagactgg aaacaagaaa
     7321 atggtatgtg gtatttttat aatactgatg gttccatggc aacaggttgg gtacaagtta
     7381 atggttcatg gtactacctc aacagcaacg gttctatgaa agtcaatcaa tggttccaag
     7441 ttggtggtaa atggtattat gtaaatgcat cgggtgagtt agcggtcaat acaagtatag
     7501 atggctatag agtcaatgat aatggtgaat gggtgcgtta acagattcct ccctgcgaca
     7561 aaattgataa tagtatgtct gaatatataa ttatatataa aaagccatag ataaggtcat
     7621 cttatagcga ctataaaaaa gtgaacaagc ctagtcttct gtacattaga attcagattt
     7681 gttcactttt tgtttaatac tcaatgaaaa tcaaagagca aactagaaaa ctagtcgcag
     7741 gttgctcaaa gtactgcttt gaggttgtag atagaactga cgaagtcagt cacatatata
     7801 atccaaggtg acgttgacgc ggtttgaaga gatttttgaa gagtataagt ctttgatttt
     7861 gagactaagg agagtcaggg tcaaagtagt gaatgattct gaaataactc ataatagtct
     7921 cttgagagga cagataagag taacaaatat ggtgctactc atgtgtcttg cattttgatt
     7981 tacaaacagc gaagaaaata taggaagctg aaataaaata tagatgaatc aatttggaga
     8041 gtcaacgcaa tctatggcaa tattttcgaa gctaggggga agcattttaa tttcaatgta
     8101 ctaggcgtct tctacagcta gctagagttt caattgtaga tgttctttga aataatatct
     8161 tagcgattta ttggtattag aagaattaga gaaattcttt ctcaataaat aaacatggcg
     8221 tcaccaaaac tgaagaagcg gtagtgttct tggatggcat ggtggtaggc atctaagact
     8281 aattcacggc ctgcaaaggc agaaaccaac atgaccagag ttgattttgg cagatggaag
     8341 ttggttgaga aggcatccac gaccttccac tcatacccag gtttgataaa gatattggtc
     8401 caaccagaat ctgcttggat ttgcccatca aacttggaac caatagtttc caaggtgcgg
     8461 atagaagtgg ttccgacagc gatgacgcga ccaccatttt ctttgacaga gcgaagggtg
     8521 gcagcagctt cctcagaaag ttgatagaac tctgagtgca tttcgtgttc gtccagatta
     8581 tccacagaaa caggtctaaa ggttccgagt ccgacatgga gagtcagata gactagatga
     8641 acacccttag cttggatttc tgccagcagt tctttggtga agtgaagacc agcagtcggt
     8701 gctgcagcag agccgctttc cttggcgtag acggtttgat aacgttcacg gtcatctaat
     8761 ttttcgtgga tataaggtgg cagaggcatt tctcccagac tttccaagac ttctaggaaa
     8821 attccttggt attcaaagcg gacaatgcgt cccccgtggg tcaattcctc tgtaacgaca
     8881 gcgctgaggc ggccatcacc aaagctgata cgagtaccga ccttgaggcg tttggcaggt
     8941 ttagccagaa cttcccactt gtctccacta gtgttcttaa ggaggagaag ttccacatga
     9001 cctcctgtct ccactttttg accatagagg cgggcaggga gaactcgggt gtcgttcatg
     9061 acaagggcat caccaggttc cagcatatca ataatagagt ggaagtgttt atcttgcatt
     9121 tctcctgtct cacggttgac gatgaggagt ttggaggcat cacgtttttc aaggggcgtt
     9181 tgggcaatca attcctctgg caagtggaaa tcaaaatcag ctgtattcat atatctgttc
     9241 attctttcta agttctaatc ctatccatta taacacgttt caagtttgga cgctcttttt
     9301 ttagaaatta aatcgttttc acttgacaaa aattggtcta taccatataa taaatataga
     9361 tagaaatgga ggatgaaaag atgaaagtta ttaaagttga aaaccaagtt caaggtggaa
     9421 aagttgcttt tgagattttg aaggaaaaat tggctaatgg agctcaaact ctaggacttg
     9481 cgacaggaag tagcccactt gaattttaca aggaaattgt tgagagtaac cttgattttt
     9541 caaatctaac cagtgtcaac cttgatgagt atgtgggcct tgatggggac aatccacagt
     9601 cttatcgtta cttcatgcaa gaaaacttgt tcaaccaaaa accatttaaa gaaagtttct
     9661 tgcctcgtgg tgttaaggac aatgctgaag ctgaagttga acgctacaac caaattttgg
     9721 ctgaccatcc agttgacctc caaatcttgg gaatcggtcg caatgggcat atcggcttta
     9781 atgagcctgg tactccattt gacagtcaaa cgcatctagt agaacttgac cagtctacta
     9841 ttgaagccaa tgcacgcttc tttgccaaga ttgaagacgt cccaacccaa gctatttcaa
     9901 tggggattaa aaacattttg gatgccaagt ctattattct ttttgcttac ggtgagtcga
     9961 aagcagaagc cattgccgga acagtatctg gcccagtgac tgagaaccta cctgcaagta
    10021 gcctccaaaa tcaccctgat gtaaccatca tcgcagatgc tgaagcgctt agcttactcg
    10081 aaaaataaaa aagcaatgtt ctattgaacg ctttcaactc ttgtataaat ggaagaaaaa
    10141 atcaaaatta aaccgcattt ttgcttgaca attattcctt ttacgtgtag aatgaaatag
    10201 atattgaact tgaagggagt gaaaaaaatg tctaaaacag tagtacgtaa gaatgaatct
    10261 cttgacgacg cacttcgccg tttcaaacgt gcggttacta aagctggtac tcttcaagaa
    10321 acacgcaaac gtgaattcta tgaaaaacct tctgtaaaac gtaaacgtaa atcagaagta
    10381 gctcgtaaac gtaaaaaatt ctaattggaa tgaaaggcta gagaaatcta gtcctttttc
    10441 ttttaaataa atactccaaa gcctgcaaaa atctgaaact tcctcctaca atttgatata
    10501 atagagagaa gaactcattt gaaggaggaa atgatgtcgg ttttagtaaa agaagtgatt
    10561 gaaaagctta gactagatat tgtctatggt gaaccagagt tgcttgaaaa ggaaatcaat
    10621 acagcggata ttacgcgacc tggtcttgaa atgacaggct attttgacta ctatacacca
    10681 gagcggattc aacttttggg gatgaaggag tggtcttatc tgatcagcat gccttccaac
    10741 agccgttatg aagttttgaa aaaaatgttt ctacctgaga caccagcagt cattgttgcc
    10801 cgtggtttgg tggttccaga ggagatgtta aaggctgcta gagaatgtaa gattgctatt
    10861 ttaaccagcc gtgcagctac cagtcgttta tctggagagt tatctagcta tctggattct
    10921 cgtttggcag aacgtaccag tgtgcacggt gtcttgatgg atatttatgg gatgggcgtc
    10981 ttgattcagg gagatagtgg aattggtaag agcgagacag gtcttgagct tgtcaaacgt
    11041 ggtcaccgtt tggtagccga tgaccgtgtc gatatctttg ccaaggatga gattactctc
    11101 tggggtgaac cagctgaaat tttgaaacac ttgattgaaa ttcgtggggt tggtattatc
    11161 gatgttatga gtctctacgg tgcgagtgct gtcaaggatt cttcacaggt tcagcttgct
    11221 gtctatttgg aaaattacga tacgcataag acctttgatc gtcttggaaa caatgcagag
    11281 gaacttgaag tttctggcgt agccattcct cgtattcgta ttccagttaa aacaggtcgt
    11341 aatatctctg ttgtgattga ggcagctgcc atgaattatc gtgccaagga aatgggcttt
    11401 gatgcgaccc gtttgttcga cgaacgactg acaagtctca tcgctcgaaa tgaggtgcaa
    11461 aatgcttgat ccaattgcta ttcaactagg acccctagcc attcgttggt atgccttatg
    11521 tattgtgaca ggcttgattc ttgcggttta tttgaccatg aaagaagcac ctagaaagaa
    11581 gatcatacca gacgatattt tagattttat cttagtagcc tttcccttgg ctattttagg
    11641 agctcgtctc tactatgtta ttttccgatt tgattactat agtcagaatt taggagagat
    11701 ttttgccatt tggaatggtg gtttggccat ttacggtggt ttgataactg gggctcttgt
    11761 gctctatatc tttgctgacc gtaaactcat caatacttgg gattttctag atattgcggc
    11821 gcctagcgtt atgattgctc aaagtttggg gcgttggggt aatttcttta accaagaagc
    11881 ttatggtgca atagtggata atctggatta tctacctggc tttatccgtg accagatgta
    11941 tattgagggg agctaccgtc aaccgacttt cctttatgag tctctatgga atctgcttgg
    12001 ctttgccttg attctgattt ttagacggaa atggaagagt ctcagacgag gtcatatcac
    12061 ggccttttac ttgatttggt atggtttcgg tcgtatggtc atcgaaggta tgcgaacaga
    12121 tagtctcatg ttcttcggcc ttcgagtgtc ccaatggctg tcagttgtcc ttatcggtct
    12181 cggtataatg atcgttattt atcaaaatcg aaagaaggcc ccttactata ttacagagga
    12241 ggaaaactaa atgttagaag ttgcatatat tcttgttgcc ctagctttaa ttgtcttttt
    12301 ggtctatctg atcattactg tacaaaagct tggtcgtgtc atcgatgaaa cagaaaagac
    12361 gattaaaacc ttgacttcag atgtggatgt gaccttgcat cacaccaatg agttgttggc
    12421 taaggtcaat gtcttggcag atgatatcaa tgtcaaggtg gctacgattg atccactctt
    12481 cagtgctgtt gcagatttat ctctatctgt ttcagacctc aatgaccatg cgcgtgtctt
    12541 gagcaagaaa gcttcatcag ctggttcaaa aacactcaag actggtgcaa gtctgtcagc
    12601 tcttcgtctt gcaagtaaat ttttcaaaaa ataaaaaagg agaatcctta tgggtaaatt
    12661 atcctcaatc cttttaggaa cggtttcagg tgcagctctt gccttgtttt taacaagtga
    12721 taagggcaaa caagtttgca gtcaggctca agattttcta gatgatttga gagaagatcc
    12781 ggagtatgcc aaggagcaag tctgtgaaaa actgacagaa gttaaggagc aggctacaga
    12841 ttttgttctg aaaacaaaag aacaggttga gtcaggtgaa atcactgtgg acagtatact
    12901 tgctcaagct aaatcctatg cttttcaagc gacagaagca tcaaaaaatc aattaaataa
    12961 tctcaaggaa caatggcaag aaaaagccga agctcttgat gactcagaag agattgtgat
    13021 tgatataaca gaagaataaa ccatcaccat ctccggacgg actatgtatc tggggatggt
    13081 gatttttatc tggaatctag tctttgtggt ataataatta ctatgcagaa aaaaccaacg
    13141 tcagcctatg tgcacatccc attttgtacc cagatttgtt attattgtga tttttcaaag
    13201 gtcttcatca aaaatcagcc agtcgacagc tatttagagc atctgctgga agagtttcgt
    13261 tcttatgata ttgaaaagtt gtcaaccctt tatatcggtg gtggaacacc gacagccctg
    13321 tcggctccgc aactggaggt gttactgaat ggcttgacta aaaacttgga cttgtctgtc
    13381 ttggaagagt tgaccattga agccaatcca ggcgatttgg atgcggataa gatagctgtt
    13441 ttgaaaaatt cggctgtcaa tcgtgtttcg ctaggtgtcc agacctttga tgataagatg
    13501 ttgaaaaaga ttgggcgcag tcatttggag aaggatattt atgaaaatat cgatcgtctg
    13561 aaactggctg gttttgacaa catctccatt gatttgattt atgctctgcc tggtcagacc
    13621 atggagcaag taaaggaaaa tgtggctaaa gccattggac tggatattcc ccacatgagt
    13681 ttgtatagtt tgattttaga aaaccatacg gtctttatga accggatgcg acgtgggaaa
    13741 ttgcctctgc ctaaggagga actagaagcg gagatgtttg agtacatcat tgcagagctg
    13801 gagcgagcgg gttttgagca ttatgagatt tctaatttct ccaaacccgg ttttgaaagt
    13861 cgtcacaatc tcatgtactg ggacaatgct gaatactatg gtattggtgc aggggcatct
    13921 ggttatgtca atggagtacg ctataaaaat catggtccca ttcgtcatta tctcagtgcg
    13981 gttgaggaag gcaatgcttg tattacagaa gatcacctga gtcaaaagga gcaaatggaa
    14041 gaagaaatgt tcttgggact ccgcaagaaa tccggggttt ccatggcgcg atttgaggaa
    14101 aaatttggac agtcttttgc tggactttat ggagaaattg tcagagattt ggttcaacaa
    14161 ggcctcatgc agattgaggg tgaccacgtt cgcatgacaa agagaggtct ctttttggga
    14221 gatactgtag cagaacgatt tattttggag taggatcatg ggcttaactt atcaaatgaa
    14281 aatgaaaatt ccttttgata tggctgatat gaacggtcat atcaaacttc cagatgtgat
    14341 tttgctgtca ctgcaagttt cagggatgca gtcgattgaa ctgggagtta gtgataaggc
    14401 cattttggaa gaatataatc tggtctggat tatcgtagaa tatgatattg aggtggttcg
    14461 tttgcctcgt tttgcggaag aaattaccat cgaaacggaa gccttgagct acaatcgact
    14521 tttttgctac cgtcgcttta ctatttataa tgaagcggag caggagctca tccacatgat
    14581 ggcgaccttt gttctcatgg accgcgacag tcgaaaagtc catgttgttg aacctgagat
    14641 tgtggctcct taccagtctg attttgataa aaaacttatc cgtggaccga agtatgagtc
    14701 cttggaagaa ccgatcagca aggattacca tgttcgtttt tacgacttgg atatgaatgg
    14761 tcatgtcaat aacagtaaat acttggactg gatttttgag gtcatgggag cggatttttt
    14821 gacccactat attcccaaga aaatcaacct caagtatgtc aaggaagttc gaccaggtgg
    14881 ggtgattaca tcggctgttg aacggactgg actggaaagc aagcatgaaa ttacaagtga
    14941 tggggttatc aatgcccaag caatcattac ttggcaagaa ataaaaaagg cttagggaga
    15001 aatagatgaa atataaaggc tatttaattg atttagacgg aaccatttat aaggggaaag
    15061 accgaatccc tgctggagag acttttgttc atgaattgca aaagcgggac attccctatc
    15121 tctttgtaac caacaataca acccgcactc cagagagtgt taaggagatg ttggctcaga
    15181 attttaatat cgatacgccc ctatcgactg tctacacagc gactttggca accatcgact
    15241 atatgaatga tttggggctt gaaaagaccg tctatgtcgt cggagaagca ggactcaagg
    15301 aagccatcaa ggcggctggt tatgtggaag acaaggaaaa acctgcctac gtggtagttg
    15361 gtctggattg gcaagtcgac tatgaaaaat ttgccacagc aactcttgct attcaaaagg
    15421 gtgcccattt tatcggaacc aatcctgacc tcaacatccc gacggaacgc ggtcttttgc
    15481 caggtgctgg ctcactgatt acgctgcttg aagtagcgac acgagtgaag ccggtttata
    15541 tcggaaaacc aaatgccatc attatggaca aggcggttga gcacttaggt ttggaacgtg
    15601 aagagttaat catggttggg gacaattatt tgactgatat ccgggctggg attgacaatg
    15661 gcattccaac gctcttggtg acgacaggtt ttaccaaggc agaagaagta gctggcctgc
    15721 caatcgcacc gacacatgtg gtttctagcc ttgcggagtg ggattttgat gaaaactaaa
    15781 ttgacctttt ggggctctat gctctttctc ctctccctct caatccttct gaccatttat
    15841 ctggcttgga ttttctatcc tatggagatt cagtggctaa acttaacgaa tcgagtctat
    15901 ctaaaaccag aaaccattca atacaatttt catatcttga tgaattatct gaccaatcct
    15961 tttagtcagg tcttacagat gcctgatttt cgttcgtcag cagctggtct gcaccatttc
    16021 gcagtggtca agaatctctt tcatttggtt cagctagtag ctctagtgac actgccaagt
    16081 ttctatgtct ttgtcaatag gattgtgaaa aaggactttt tgtctcttta tcgaaaaagt
    16141 ctcctggctc tagtagtctt acctgtgatg attggacttg ggggagtttt gattggtttt
    16201 gaccaattct ttactctttt ccatcaaatt ctctttgtgg gagatgatac ctggcttttt
    16261 gatccagcca aggatcctgt tattatgatt ttgccagaga ccttctttct tcatgccttc
    16321 ctcctctttt ttgccctcta tgaaagcttc tttggctatc tgtgtctgaa aagtcgtagg
    16381 aagtgaaatg aaagatgttt ttaatttgca tatataaaaa tatatatcgt ttaaaaccat
    16441 cgttaagagt gaattaacca aatccaattg tgtcaatcgt tcgcaaacag cctatttttc
    16501 ttgaaaaaat aggctttttt tctaaaaatc cctagtcaag cgccttattt ttttgtataa
    16561 tagaagtagc aaagtataga taagaagaga aaagcatgat tacactattt ctatcaccga
    16621 gctgtacatc atgtcgtaag gctaaggcct ggttagaaaa acataaggtt ccctttgtgg
    16681 aacacaatat tatgaccagt cctttaacaa gaaaagaatt gcaacacatc ctttccttga
    16741 ccgaaaatgg tactgatgac atcatttcaa ctcgttcaaa aatttttcaa aaattgaata
    16801 ttgatgtaga gagtatttcg gtatcggaat tgcttcattt gattgagcag tatcctagtc
    16861 ttttgcgtcg tccaattatt attgatgcca agcgtatgca aatcggtttt aatgaagatg
    16921 agattcgtgc ttttctccct cgtagttatc gtaagcaaga actaaaagaa gcaagaatga
    16981 gagctggtat tagttaatga acaaacagta tagttaccca ctagatttgt cgtggagcac
    17041 tgaagaactt gcttcagtgc tttctttttt taatgatgtt gaagctgcct atgaaggcaa
    17101 agtagaggct aaaaagttac tggactccta taagggattc aaagcggtcg tgccaagcaa
    17161 gagcgaagag aaacgtttgg gacgagaatt tgaaacggtt agtggctatt cactttaccg
    17221 agcagttcag gctgccaaag aaaaagggga aggaaagatt tctcttggaa agtaaatttg
    17281 aatttgctaa agagcttgtt aagaaagcag gtcagtacat tcttgaccat atgcaggaag
    17341 acttgcgtgt tgaaaccaag tcctctccaa cagatttggt gaccagactg gacaaagaag
    17401 ttcaggaact cttggttggt gagattttgt cccgttatcc tgaggataag atttgtgctg
    17461 aagaaggttg tctgcgagcc tcggttcaag agggcaaggt ttgggtcatt gatcccattg
    17521 atggtaccaa taattttgta gcccagcagg aagattttgc tgttatgatg gcttattttg
    17581 aaaatggtca gggacagttt ggtctgattt atgatgtggt caaaggggat tgttaccacg
    17641 gtggtgggga atttccagtt tgtctaaatg ataggcccct agctcctttt aaaactaaac
    17701 cacttggaga ttttctcatt gcagggaata gtggtatgct ggaaaccaat gaatgggggc
    17761 tggctgattt gagccgagcg gttcttggtg ttcgtgtcta tggaagtgcg gccattagtt
    17821 ttgccaagat tttgtcaggg cgtttgttga cctatctcac ttatctgcaa ccatgggatt
    17881 atgccgcagc tagtatttta ggggaaagtc tgggctatcg ggttgtgacc ctttttggtg
    17941 aagctcctga ttttcaaacc agacagccgg tcatgatggt gcctcttgag atgcaggagg
    18001 aaattcagtc ctatatttac gaaaggaaaa gaacttaaat gcaatttcca gaaggatttg
    18061 ttgaaaaata taaagagata ctaggagatg aggcaagaga ttttcttgcc tcttttgagg
    18121 aggaagcggt ttcggccttt cgggtcaatc ccttaaaaga agagcaactt tccttttctg
    18181 atgccattac tcaaacccct tggggccact atgggaaggt ttcagggaaa tcgcctgagc
    18241 atgctacagg tttagtttat tcgcaagaac ctgctgctca aatggtggct caagtagctc
    18301 aacccagtcc tggtatgaag gtcttggact tggctgctgc accggggggc aaatcaactc
    18361 aactggcagc ctatctagca ggggaggggc tacttgtttc caatgaaatt tcaagcaaac
    18421 gggctaagat tttggttgaa aacatggagc gttttggagc gacaaatgtc gtggtgacca
    18481 atgaatctgc cgaccgcttg gtcaaggtct ttaaaggcta ttttgactta atcgttcttg
    18541 atgccccttg ctctggtgaa ggaatgtttc gtaagcaacc tgatgctatg gactattgga
    18601 gcttagatta tccgagtcaa tgtgctagct tgcaaagaga aattctggag gatgcagtga
    18661 ccatgttagc tgaaggtggt cgtctggttt attcgacctg tacctgggca cccgaggaaa
    18721 acgaagagat tgtcaattgg ttgctggaag agtatgattt tgacttgttg ccagtagagc
    18781 atatcaatgg aatggtagct ggtattgacc tgccagaaac ggctcggatg taccctcatc
    18841 agtttaaggg agagggtcag tttgttgccc atctacagtt taagggtaac aatccagctc
    18901 ctaaatttaa ggcaagtaag agtaacctca gccgtgaaca agttgccttg tggcaggaat
    18961 ttgcccaaaa ccatttgaag gtcaatctac cgggtatctt gcagactttt ggagaccagc
    19021 tttatctctt gccagaactt ttgccagatt tagggaagct caagattgct cgcaatggac
    19081 tgcatctggg cacttttaag aagaaacgct ttgagcccag tttcgctctt ggtttagcct
    19141 tgaaaccgag tcaggtcgaa cagtcggttg aaattggcca agaagcgttt gtcaagtatg
    19201 cggctggaga aatcgttcag ctggcagaaa gtctgccaaa tggttggtac caagttttgg
    19261 ttaaaggcaa tggcttgggc tttgcaaagg ttactggaaa tgttttgaaa aattattttc
    19321 caaaaggcct cagattcaaa tgaaaatgct agtcaaatat cttattctat gttatagtga
    19381 aagtatggat ttttttagta ggatgattct tgatatcttg attgccatta catgtagttt
    19441 tactactcaa tgaaaatcaa agagcaaact aggaagctag ccatagatag ctcaaaacac
    19501 tggtttgaag agattttcaa agagtacaag aagtttagtt attagcgttc ttaccgcttg
    19561 taaactagat ttctcataaa atagaatctt ttccttttag ttgtaaacta gtctgggaga
    19621 gtagagaggt ttgagatacc tttctagctt ttggattatc atctaaggag agtaatttcc
    19681 cttgcattaa aaaggggaaa aagagacacg aaatgactat aatgggtgac aatgggggaa
    19741 gggatagaca agagatttta tccacatatg aaaaaaggag gttaggaaag agtaatatag
    19801 cctatattat ataaataatc aattgcgcag aaatttggta agaattcatg cgtcaactca
    19861 taaagaacta cttaaaaaat tcacagtatt cataattatt ttcgaggaga aaaacagtga
    19921 aaaaaagaaa aaagcttgct ctgtctctta tcgctttttg gctgacggct tgtttagtag
    19981 gctgtgctag ctggattgat cgtggagaat ccataacggc tgttggctca actgccttgc
    20041 aacccttggt tgaagtagcg gcagatgaat ttggcacgat ccatgttgga aaaacggtca
    20101 atgtccaagg gggaggttct ggtacaggct tgtcccaggt tcagtctggg gcagttgata
    20161 taggaaactc agatgtattt gctgaggaaa aagacggaat tgatgcttct gctcttgttg
    20221 accacaaggt cgcggtagct ggcttggctc tgattgtcaa taaggaggtt gatgttgata
    20281 acctaacgac agagcaactt cgtcaaatct tcataggtga ggtaaccaat tggaaagagg
    20341 ttggtggtaa ggacttaccc atctctgtta tcaatcgggc agccggctct ggttctcgtg
    20401 ctacctttga tactgtcatt atggaaggtc agtctgccat gcaaagtcag gagcaggatt
    20461 caaatggagc ggtaaaatca atcgtatcaa aaagtccagg agctatctct tatttatctc
    20521 ttacctatat agatgattcg gtcaaaagca tgaagttgaa tggctatgac ttaagtccag
    20581 aaaatataag tagcaataat tggcccttgt ggtcttatga gcatatgtat acattggggc
    20641 agcccaatga gttggctgca gaatttctca attttgttct ctcggatgag acccaagaag
    20701 ggattgtcaa aggattgaag tatattccga ttaaggaaat gaaggttgaa aaagatgctg
    20761 ccggaactgt gacagtgttg gaagggagac aataatgaat caagaagaat tagctaagaa
    20821 aatgttgctt ccatcaaaga attctcgtct ggagaaatta ggaaaaggtt tgacctttgc
    20881 ctgtctttct ttgatagtca tccttgtggc catgattttg gttttcgtag cgcaaaaagg
    20941 cttgtcgacc ttctttgtca atggtgtgaa tatctttgac tttcttttgg gaggaacttg
    21001 gaatccttct agtaaagaat ttggtgccct tcctatgatt ttgggttcct ttatcgttac
    21061 cattctctca gcccttatcg caacaccctt tgctattggt gcagcagttt ttatgaccga
    21121 agtatcacca aaaggggcga agattttgca accagctatt gaactcctgg ttgggattcc
    21181 ttcagtagtg tacggattta ttggcttgca agtcgtcgtt ccctttgttc gcagtgtctt
    21241 tggtgggact ggttttggga ttttgtcagg gatttccgtc ctctttgtca tgattttgcc
    21301 gaccgtaacc tttatgacaa cggatagctt gcgtgcggtt cctcgttatt atcgtgaggc
    21361 cagtttcgct atgggagcca ctcgctggca gactatctgg cgtgtgatct tgaaggcggc
    21421 ccgttctggt attttcactg cagtggtctt tgggatggcg cgtgcctttg gtgaggcttt
    21481 agctatccag atggttgtcg gaaactcagc tgttatccca acttccttga ccacaccagc
    21541 tgcaacttta acttctatat taactatggg aattgggaac actgtcatgg gaactgtaaa
    21601 taataatgtt ctctggtcac tggccttggt actgctcttg atgagtttag tttttaacag
    21661 tgtgattaaa ttgattacga aagaaagagg aaagaaaaat tatgcgcgct aagaaattag
    21721 ataaacttgc aacagctgtc ctctatacga ttgctagcat cattgtgaca atcttggctt
    21781 ccttaattct ctatatcttg gttcggggct tgccccatat ctcttggtct ttcttgactg
    21841 gaaggtcttc tgcttttcaa gcaggtggtg ggattggcat tcagctttac aattcctttt
    21901 tcctattggt cattaccttg attatttctg tacctctttc tatgggagct gggatttact
    21961 tggctgaata tgctaaaaaa ggtcctgtta ccaactttgt gcggacttgt attgaaatct
    22021 tgtcctcttt accatcagtg gtggtgggtc tctttggtta cttgatcttt gtagtccagt
    22081 ttgagtatgg gttttcaatc atttcaggtg ccttggcctt gacagtcttt aacttgcctc
    22141 agatgacgcg taatgtagag gatagtttga aacacgttca ccatacccaa cgtgaggctg
    22201 gtctggctct tgggatttct cgctgggaga cagtggttca tgttgttatt ccagaagcgc
    22261 ttccaggtat tgtaacgggt gtcgtcttgg catctggtcg tatctttggc gaagctgcag
    22321 ctctgatcta tacagcaggg caatcggcgc cagctcttga ctggtctaac tggaatatcc
    22381 tcagtgtgac tagccccatc tctatcttcc gtcaagcaga aaccttggct gtccatatct
    22441 ggaaagtcaa tagtgaaggc actattccag atggaaccat tgtatcagca ggttctgccg
    22501 ctgtgctcct gatctttatc ctgattttta actttggagc tcgtaagttc ggaatctatc
    22561 tacacaagaa attaaccgct gcctaaagga gaagccatgt caacatataa ctgggatgag
    22621 aagcatatcc ttacctttcc tgaagaaaaa gtagcccttt ctactaagga tgtccatgtt
    22681 tactatggta aaaatgaatc cattaagggg attgatatgc aatttgaaag aaataaaatt
    22741 acagctttga ttggtccgtc gggatcgggg aaatctacct acttacgcag tctcaatcgc
    22801 atgaatgata ccattgatat tgctaaagta actgggcaga ttctctatcg tggaattgat
    22861 gtcaaccgtc cagaaatcaa cgtttatgaa atgcgtaaac acattggaat ggtttttcaa
    22921 cgccccaatc catttgctaa gtcaatttac cgtaatatta cctttgcgca tgaacgtgct
    22981 ggagttaagg ataagcaagt cctagatgaa atcgtagaaa cctcccttcg tcaggctgcc
    23041 ctctgggatc aggttaaaga cgatctccac aagtcagcct tgaccttatc aggtggtcag
    23101 caacaacgtc tctgtatcgc tcgtgccatc tctgttaagc cagatatcct cttaatggat
    23161 gagccagcct cagccttgga tccgattgcg accatgcaac tagaagagac catgtttgag
    23221 ctcaagaaaa actttaccat catcattgta acgcataata tgcagcaggc tgctcgtgca
    23281 agtgactata caggcttctt ttacttgggt gatttgattg agtatgacaa gactgcaact
    23341 attttccaaa atgccaagct acagtccacc aatgactatg tatctggtca ctttggttag
    23401 aaaggaaacc gtatgacaga tgcgatttta caggtatcag acctgtccgt ttattataat
    23461 aaaaagaagg ctttgaatag tgtttcccta tctttccaac ctaaggaaat tacagccttg
    23521 attggtccat ctggatcagg gaagtcaacc ctcctcaagt ctctcaaccg catgggagat
    23581 ctcaatccag aggtgaccac aactggatcc gtggtgtaca atggtcacaa catctacagt
    23641 ccgcgtacag atacggttga attacgtaag gaaatcggaa tggttttcca acaacctaat
    23701 cctttcccta tgactatcta tgagaatgtt gtctacgggc ttcgtatcaa tggaattaag
    23761 gataagcagg ttctggatga agccgtagaa aaagccttgc aaggtgcctc tatctgggat
    23821 gaggtcaagg atcgtctata tgattcagct attggattgt caggtggtca acagcagcgt
    23881 gtctgcgtgg cccgtgtctt ggcaactagt cctaaaatca tcctcttgga tgagccaact
    23941 tcggctttgg atccgatttc agctggtaaa attgaggaaa ccttgtatgg tctaaaagac
    24001 aagtacacca tgcttctggt aacccgttcc atgcagcaag cttcacgtat ctctgataag
    24061 acaggatttt tcctagatgg agatttgatt gaatttaatg ataccaagca gatgttcctt
    24121 aatccccaac acaaggaaac ggaagactat attacaggaa aatttggata aggagatgaa
    24181 agatgttacg atctcaattt gaagaagatt tagagaaatt acataaccag ttctacgcta
    24241 tgggacaaga agtgctctca caaatcaatc gtacggtacg tgcttttgtc acgcatgacc
    24301 gtgacctggc aaaagaggtc atcgaagatg atgcagaagt aaatgaatac gaagtgaaac
    24361 tggaaaagaa atcatttgaa atgatcgcac tccaacaacc agtctctcaa gatttgcgta
    24421 cagtcttgac tgtccttaag gctgtatcag atgtggagcg tatgggggat cacgctgtag
    24481 ccattgctca ggcaaccatc cgtatgaagg gggaagagcg cattccagct gtagaggaag
    24541 aaattaaaaa aatgggacgt gaagttaaaa gcgttgttga agcagcactt gatctttatc
    24601 ttaatggttc tgttgacgac gcataccggg tggcctccat ggatgagcaa attaaccact
    24661 attttgaaac tatccgtgac cttgcgactg aagagattaa gaaaaatcca gaagccattg
    24721 ttacgggtcg tgattatttc caagttattt cctacttgga gcgtatcggt gactatgcta
    24781 aaaatatctg tgaatgggtt gtctactttg aaacaggtaa gattgtcgaa ctataaaata
    24841 gtttaattct gtataaaaag gagcttgaaa tcaactgatg gctccttttt ttgaacgaaa
    24901 aaatattttt gagataaaaa tttaaaaaac acttgacaag
//
LOCUS       DAVKLW010000028        24649 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKLW010000028 DAVKLW010000000
VERSION     DAVKLW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.028"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VW324_001148"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VW324_001148"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9726797.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVEYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VW324_001149"
     CDS             complement(1169..1693)
                     /locus_tag="VW324_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9726798.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRK
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VW324_001150"
     CDS             complement(1746..1982)
                     /locus_tag="VW324_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9726799.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VW324_001151"
     CDS             complement(2050..2970)
                     /locus_tag="VW324_001151"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9726800.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEIYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3565
                     /locus_tag="VW324_001152"
                     /pseudo
     CDS             3160..3565
                     /locus_tag="VW324_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3781..4362
                     /locus_tag="VW324_001153"
     CDS             3781..4362
                     /locus_tag="VW324_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9726801.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4359..5636
                     /locus_tag="VW324_001154"
     CDS             4359..5636
                     /locus_tag="VW324_001154"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9726802.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5654..6316
                     /gene="deoC"
                     /locus_tag="VW324_001155"
     CDS             5654..6316
                     /gene="deoC"
                     /locus_tag="VW324_001155"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9726803.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGSDFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6303..6692
                     /locus_tag="VW324_001156"
     CDS             6303..6692
                     /locus_tag="VW324_001156"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9726804.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6782..7834
                     /locus_tag="VW324_001157"
     CDS             6782..7834
                     /locus_tag="VW324_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9726805.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7978..9513
                     /locus_tag="VW324_001158"
     CDS             7978..9513
                     /locus_tag="VW324_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726806.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVVDISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            9506..10564
                     /locus_tag="VW324_001159"
     CDS             9506..10564
                     /locus_tag="VW324_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726807.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10567..11523
                     /locus_tag="VW324_001160"
     CDS             10567..11523
                     /locus_tag="VW324_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726808.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            11608..12724
                     /locus_tag="VW324_001161"
                     /pseudo
     CDS             11608..12724
                     /locus_tag="VW324_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(12767..13408)
                     /gene="plsY"
                     /locus_tag="VW324_001162"
     CDS             complement(12767..13408)
                     /gene="plsY"
                     /locus_tag="VW324_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9726809.1"
                     /translation="MITIILLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            13544..15487
                     /gene="parE"
                     /locus_tag="VW324_001163"
     CDS             13544..15487
                     /gene="parE"
                     /locus_tag="VW324_001163"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9726810.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KVGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRSDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            15907..18378
                     /gene="parC"
                     /locus_tag="VW324_001164"
     CDS             15907..18378
                     /gene="parC"
                     /locus_tag="VW324_001164"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9726811.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            18510..19532
                     /locus_tag="VW324_001165"
     CDS             18510..19532
                     /locus_tag="VW324_001165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9726812.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            19588..20292
                     /locus_tag="VW324_001166"
     CDS             19588..20292
                     /locus_tag="VW324_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9726813.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            20357..21046
                     /locus_tag="VW324_001167"
     CDS             20357..21046
                     /locus_tag="VW324_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9726814.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            21043..21966
                     /locus_tag="VW324_001168"
     CDS             21043..21966
                     /locus_tag="VW324_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9726815.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            21981..22625
                     /gene="pcp"
                     /locus_tag="VW324_001169"
     CDS             21981..22625
                     /gene="pcp"
                     /locus_tag="VW324_001169"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9726816.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            22793..23023
                     /locus_tag="VW324_001170"
     CDS             22793..23023
                     /locus_tag="VW324_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9726817.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            23073..23153
                     /locus_tag="VW324_001171"
     tRNA            23073..23153
                     /locus_tag="VW324_001171"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23107..23109,aa:Tyr,seq:gta)
     gene            23163..23234
                     /locus_tag="VW324_001172"
     tRNA            23163..23234
                     /locus_tag="VW324_001172"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23195..23197,aa:Gln,seq:ttg)
     gene            23396..24598
                     /gene="rpsA"
                     /locus_tag="VW324_001173"
     CDS             23396..24598
                     /gene="rpsA"
                     /locus_tag="VW324_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9726818.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT         7409 a         4346 c         5602 g         7292 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg atgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcaggt tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttggtgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag aatacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctttctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt taatggcgaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgcgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc tacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttgattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctc ttcgggtacg atgtcgtaaa tttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtt aaaggaggtg ttgtttttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatataa
     3061 tataagtgat caatttattg atgaaatagg aggtagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg atgtgtacta tgaaaatctt aaaatgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg agccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tatggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgctc ttaattgctt tcttactaaa aaaaacgatg
     3361 ggcgaaatgg attgctgctg gtagttattg ctttttagtc ctatggattg cgacagaggg
     3421 ttatttcttc cacatgtcac tagaagattt gatacgactt tggacaagtt tggaaatcct
     3481 gacacaaacg tatcagttgg gcttttatct aaatattttg ttaggaattt tgttgatgat
     3541 taagcatttg aaggttaagc aatagtaatc aaaatgcgcc tgatgataga caataaactt
     3601 tttgttttta tctggactgt gatatttata gttctaaatg gtttattatt tataagtgtt
     3661 gaaagagatt gaagaaaatg gcttcgtgac tcgctttctt cggtcttttt cgttttatta
     3721 tcaatttgta aacgatttac aatttgagtc aaattatggt aaaatagtgt catgagtaaa
     3781 atgtattatg cagaaaatcc tgacgctgct cacgacattc atgagttgag agtagagttg
     3841 ttgggacaaa aaatgacctt tttgacggat gcgggtgttt ttagcaagaa aatagttgac
     3901 tttggaagtc aactcttgct caagtgtctg gatgtcaacc aaggagagac agtcctagat
     3961 gtaggctgtg gttatggtcc attaggtttg tccttggtca aggtttacgg agttcaggcg
     4021 accatggttg atattaacac tcgtgccctg gatttagctc ggagaaatgc tgaaaaaaat
     4081 aatgcaaaag cgacgatatt tcaatctaat atttatgcaa gagttcaagg gcgatttgac
     4141 catgtcattt ccaatcctcc tatccgtgcg ggcaagcaag tcgttcatga aatcatcgag
     4201 aaaagtaaag atttcttgga aactggtggg gatttgacaa tcgtcatcca gaaaaaacaa
     4261 ggggctccaa gtgctaagtc caagatggag gatgtgtttg gtaattgtga aatccttaaa
     4321 aaagacaaag gatactatat ccttagaagt gtgaaagaat gagagcagtt gatttaatcc
     4381 aaaaaaaacg agacggtcaa gaactgactt caagtgaaat tgaatggctg atagaaggct
     4441 atgtgtcagg aactgttcct gattatcaga tgtctgcctt tgctatggct gtttatttta
     4501 aaggaatgac gactcgtgaa atttctgatt taacgatgaa tatggttaag actgggcaag
     4561 agtttgacct gtcagccatt gatggtgtga aagttgacaa gcattctact ggtggtgtcg
     4621 gcgataaggt aaccttgatt ttagctcctc ttgttgctag cttcggtgtg cctgtagcaa
     4681 aaatgagtgg tcgtggtctt ggccatacag gcggaacaat tgataaattg gagtccatta
     4741 agggctatca agtagaacgt agtcaagagg attttattcg tcaggttcag gatattggag
     4801 tatctgtcat tgggcaatca gaccagctgg tcaaagcaga taagcttctc tatgcccttc
     4861 gtgatgtgac cgcaactgtc gacacgattc ctttgattgc gagttcggtg atgagcaaga
     4921 aaattgcggc aggggcggat gctattttgc tagacgtgac tgtcggtgag ggtgccttca
     4981 tgaagacggt tgatgaggcg cgtgagttgg ctcaaactat ggtagatctt ggtaaggttg
     5041 ttggacggaa gacagtagca gtgattacgg atatgagcca gcctttggga cgagcgattg
     5101 gaaatcgtct ggaaatcctt gaagcattgg agattttgca aggtcaagga cgtcaggata
     5161 ttacccactt tatttgtgaa ttggctcaaa ttatgcttgg tttggcaaat gttaacaaaa
     5221 ctgttgaaga agttcgccaa catcttgaga atggtcaagc actggctaag tttgaggaaa
     5281 tggtgcaagc ccaaggtgga gacttggaag acctctattg tcctgtaaat gtagctcatg
     5341 tggtggaaat ccctgctcag gaaacgggtg tcatttcagc tcttccagct atggactttg
     5401 gtctttatgc catgaggctg ggtgctggtc gtgcagttaa gtctgatgcc ttggactatg
     5461 aaacaggaat tgtttttgaa aagaagattg gagactccgt tcaaaagggt gaaattgttg
     5521 caaaagtata cacaaatgaa aaaattccac ctcaactagt tacagatttc caaaaatgtg
     5581 ttaaaataag tgatgaagtg aaaaaaatcc gtgaaattgt agaaatcatc tcctaaatgg
     5641 agggaaaacc gaaatgaaat taaataaata tatagatcat acgcttttaa aacaagatgc
     5701 aaagaaaaaa caaattgata gtttgttgtc tgaggctaga gagtatgact ttgccagtgt
     5761 ttgcgttaat ccgacctggg ttgaacatgc taaaaaagga cttgaaggca cagatgttaa
     5821 ggtttgcaca gtagtaggtt tccccttggg agcaacaact tcaactgtga aagcttttga
     5881 aacaaaagaa gctatccaaa atggggctga tgagattgac atggtaatca atgttggggc
     5941 tctcaagtca ggtgatttgg ccttggttga gtcggatatt cgtgcagtag tggaagcaag
     6001 tggggacaag ttagtgaaag tcattattga agcttgcctt ctgacagacc aagaaaaagt
     6061 tttggcttgc caattagccc aaaaagcagg atctgacttt gtcaaaacat ctactggctt
     6121 ttcaactggt ggtgctacga tagcagatgt tagattaatg cgtgaaacag ttggatctga
     6181 tatgggagtc aaggctgctg gtggagctcg ttcttatgca gatgctcttg cctttgtcga
     6241 agcaggtgcg acccgtatcg gaacgtcagc tggggtagct attttaaaag gagaattggc
     6301 agatggcgac tactgagttg attgaactgg caattgaaac cagcaaacat gcctatgtcc
     6361 cctattctca ctttcctatc ggagcagttt tagtagccaa agacggaagt gtttacacgg
     6421 gagtgaatat cgaaaatgct agctatcctt tgaccaattg cggtgagaga acagctattt
     6481 ttaaggctat atctgaaggc caaagagaat tttcagaatt gattgtctat ggtcaaactg
     6541 aaaaaccgat ctcgccatgt ggtgcttgtc gccaagtgat ggctgaattt tttgaacaag
     6601 atctaaaagt gaccttagtc gcaaaagata aatcgacggt cgagatgacg gtcggggaat
     6661 tacttccata ctcttttaca gacttaaact agtctgagtc gctctctgag tggcacggtc
     6721 cttgtggcca accaatccat acttgcaaca tcgttgcaca tcttatttag gaggttcagt
     6781 aatgaacaag aaacaatggc taggtcttgg cctagttgca gtggcagcag ttggacttgc
     6841 tgcatgtggt aaccgctctt ctcgtaacgc agcttcatct tctgatgtga agacaaaagc
     6901 agcaatcgtc actgatactg gtggtgttga tgacaaatca ttcaaccaat cagcttggga
     6961 aggtttgcag gcttggggta aagaacacaa tctttcaaaa gataacggtt tcacttactt
     7021 ccaatcaaca agtgaagctg actacgctaa caacttgcaa caagcggctg gaagttacaa
     7081 cctaatcttc ggtgttggtt ttgcccttca caatgcagtt gaagaagcag ctaaagaaca
     7141 cactgacttg aactatgtct tgattgatga tgtgattaaa gaccaaaaga atgttgcgag
     7201 cgtaactttc gctgataatg agtcaggtta ccttgcaggt gtggctgcag caaaaacaac
     7261 taagacaaaa caagttggtt ttgtaggtgg tatcgaatct gaagttatct ctcgttttga
     7321 agcaggattc aaggctggtg ttgcgtcagt agacccatct atcaaagtcc aagttgacta
     7381 cgctggttca tttggtgatg cagctaaagg taaaacaatt gcagccgcac aatacgcagc
     7441 cggtgcagat attgtttacc aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc
     7501 aaaatctctc aacgaaagcc gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgaccg
     7561 tgaccaagaa gcagaaggta aatacacttc taaagatggc aaagaatcaa actttgttct
     7621 tgtatctact ttgaaacaag ttggtacaac tgtaaaagat atttctaaca aggcagaaaa
     7681 aggagaattc cctggcggtc aagtgatcgt ttactcattg aaggataaag gggttgactt
     7741 ggcagtaaca aacctttcag aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat
     7801 ccttgatgga agcgtaaaag ttcctgaaaa ataatggatg gaagtcattt cttaggaagc
     7861 ggccaagggc cgctttttta agagttgaga caaagtcatt ttgtctcagt aaagcttgct
     7921 actagacaaa ctagcaagtt ttattgaaat aaaataagac tctgaaagga agagcacatg
     7981 gcacacgaaa atgtcattga gatgcgtgat attaccaagg tgtttggtgg atttgttgcc
     8041 aacgacaaaa tcaacttgca cctacgaaaa ggtgaaattc atgcactttt aggagaaaat
     8101 ggggctggta agtccacgct aatgaacatg ttagcaggcc ttcttgaacc aactagtggt
     8161 gaaatcgcgg tcaacggtca agttgtcaat ctcgactccc catctaaagc agctagcttg
     8221 ggaatcggga tggttcacca gcactttatg ttggttgaag ccttcacagt ggctgaaaac
     8281 atcattttag gtagtgaatt gactaaaaat ggtgtgctag atatcgctgg agctagcaaa
     8341 gaaatcaagg ctctttctga acgttatggc ttagctgttg acccttctgc caaggtagta
     8401 gatatctcag ttggagccca acaacgtgta gaaattttaa aaacacttta taggggggct
     8461 gatatcctta tctttgacga accaacggct gttttgactc catcagaaat tgatgagttg
     8521 atggctatta tgaaaaatct tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa
     8581 ttggatgaaa ttcgcgcagt ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt
     8641 gaaaccgttg aaattgcagg ggctaccaat gctgatttgg cggaaatgat ggtaggacgt
     8701 tctgtttcct ttaaaacaga gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc
     8761 aaagatttgg tggtcaatga aaaccgtggt gttccagctg ttaaaaatct gtccttggat
     8821 gttcgtgctg gagagattgt tggtattgcg gggattgatg gaaatggtca gtctgaactg
     8881 attcaagcca ttacaggtct tcgtaaggtt gaatctggta gcattgagct aaaaggagat
     8941 tcaattgtag gcttgcaccc acgtcagatt acagaactaa gtgttgggca cgttccagaa
     9001 gaccgtcacc gtgatggctt gattttggaa atgatgatat ctgaaaatat tgcccttcaa
     9061 acctactata aagaaccaca tagtaaaaat ggaattttga attattcaaa tattacttct
     9121 tatgctaaaa agctgatgga agagtttgat gttcgcgctg ccagtgaatt agttcctgca
     9181 gctgcactct caggaggaaa tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat
     9241 cctgatctcc ttatcgttag ccagccaact cgtgggttgg atgtcggtgc cattgagtat
     9301 atccacaaac gcttgattga agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt
     9361 gaattggatg aaattttaaa cgtctcagac cgtattgccg ttatccacga tggtaagatt
     9421 caaggtattg tatcaccaga aacaaccaat aaacaaggac ttggtgtctt gatggctggt
     9481 ggaaacttgg gaaaggagaa gagtgatgtc taaaaaatta caacaaattt cggttccctt
     9541 gatttctgta ttcctaggaa ttttactcgg agccattgtc atgtggatct tcggttatga
     9601 tgctatttgg ggctacgaag aattgttcta tacagccttt ggcagtctgc gtgggattgg
     9661 agaaatcttc cgtgctatgg gtcctctggt cttgattggt cttggttttg cggttgccag
     9721 tcgagctggt ttctttaacg tcggacttcc tggtcaggct ttggcaggtt ggattctcag
     9781 tggttggttt gccctgtcgc atccagatat gccccgtccc ttgatgattc tagcaaccat
     9841 cgtgattgcc ttgattgctg gtgggattgt cggagcgatt ccaggtattc ttagggccta
     9901 tctagggacg tcagaggtta ttgtaaccat catgatgaac tacattgtct tgtatgtagg
     9961 gaatgccttt atccatgctt tccctaaaga cttcatgcaa agtacagatt cgaccattcg
    10021 tgttggggct aatgcaacct atcagacacc ttggttggct gagttgactg gtaactcacg
    10081 gatgaatatt ggtattttct ttgccatcat tgccgttgca gttatttggt tcatgctcaa
    10141 gaaaacaact cttggttttg aaatccgtgc agttggtctt aatccacatg cttcagaata
    10201 tgctggtatt tctgccaagc ggactattat cctatctatg attatttcag gtgccttggc
    10261 aggtcttggt ggagctgttg aaggtttggg aaccttccag aacgtctatg ttcaaggttc
    10321 gtcattagct atcggattta acggaatggc ggttagtttg cttgcggcca actcaccaat
    10381 tggtatactc tttgcagcct tcctatttgg cgttctccaa gttggggctc ctggtatgaa
    10441 tgcggcgcag gtaccatctg agcttgtcag cattgtaaca gcgtctatta tcttctttgt
    10501 cagtgttcat taccttatcg aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa
    10561 gtaaagatgt ctattataac cttgctcccc ctcttggtgt cttctatgct gatttactca
    10621 gcacctctca tctttacaag tatcggtggt gttttctctg aacgtggtgg tgtggtaaac
    10681 gttggccttg aaggaattat ggttatgggt gccttttctg gagttgtctt taaccttgaa
    10741 tttgcagaac aatttggagc agcaactcca tggctatcct tgcttgtagc aggattggtt
    10801 ggtagtgttt tctctatcat ccacgcagca gcgacggttc atttccgtgc agaccatgtt
    10861 gtcagcggta cggtattgaa cttgatggcg cctgccttgg ctgttttctt agttaaagtt
    10921 ctttataaca aaggacaaac cgacaaccta agtcaaactt ttggacgctt tgatttccca
    10981 gtcttggcaa atatcccagt gattggtgat atcttcttca agtcaactag tctacttggt
    11041 tatctggcga ttgccttctc attccttgct tggtttattc tctttaagac tcaatttggt
    11101 cttcgtctcc gctctgtcgg tgaacatcct caagcagcgg ataccttggg aatcaacgtc
    11161 tacaagatga gatatttagg ggttattatt tcaggttttc taggtggaat tggcggagcg
    11221 atttatgcgc aatcaatctc agttaacttc tcagtgacaa ctattgttgg acctggattt
    11281 atcgcccttg ctgcgatgat ctttgggaaa tggaatccaa tcggtgctat gctttctagt
    11341 ctcttctttg gactttcaca aagtttggct gttatcggtt ctcaattgcc gttcctacaa
    11401 ggagtgccag cggtttatct tcaaattgca ccttatgttt tgacaattct tgtcttagca
    11461 gccttctttg gaaaagcagt cgcaccaaaa gcagatggta tcaactacat caaatcaaaa
    11521 taagcataca aaaaaacgtc agtccaatga ctgaactgca ccccaaaagt tagacagaaa
    11581 aaatctaact tttggggtgt ttttattatg aaattaagtt atgatgataa agttcagatc
    11641 tatgaactaa gaaagcaagg atatagctta gagaagcttt caaataaatt tgggataaat
    11701 aattctaatc ttaggtatat gattaaattg attgatcgtt acggaataga gttcggcaaa
    11761 aaaggaaaaa atcgttacta ttctcctgat ttaaaacaag aaatgattca taaagtctga
    11821 catgaaggct ggactaaaga tagagtttct cttgaatacg gtctcccaag tcgtacgata
    11881 cttcttaact ggctagcaca atacaggaaa aacgggtata ctattgttga gaaaccaaga
    11941 gggagagtac ctaaaatggg acgtaagcca agaacgagac ctgaagagag gacagaatta
    12001 gaacgtcttc aagcagaaaa tgagtacctg agagcggaga atgccatcct aaaaaagtta
    12061 agagaactcc gattgaagga ggaaaaagag aaagaagaaa gacagaaatt gttcaagaat
    12121 taatgactga gttttcgtta gatcttcttc taaaagccat taaactagct cgttggacct
    12181 actactatca cttgaaacag ctagacaaaa cagataagga ccaagagctt aaagctgaaa
    12241 ttcaatccat ttttatcgaa cacaaaggaa attatgctta tcgccgggtt catttagaac
    12301 taagaaatcg tgcttatctg gtaaatcata aaagagttca aggcttgata aaagtactca
    12361 taatttagaa caagtacaaa caatgttgga acaggcattc aaagagaagc actacgagaa
    12421 tacgattctc catagtgacc aaggctggca atatcaacac gattcttatc atcggttcct
    12481 agagagtaag ggaattcaag catccatgtc acgtaagggt aacagcccag acaatggtat
    12541 gatggagtcc ttctttggga ttctgaaatc ggaaatgttt tacggttatg agaagtcgtt
    12601 tcagtcgttt aagcaattgg aacaagctat tgtagactat attgattact acaacaacaa
    12661 acgaattaag gtaaaactaa aaggacttag tcctgtgcag tacagaacta aatcctttgg
    12721 ataaattatt tgtctaactg tttgggggca gtacagaact ggcgttttat tttttaggat
    12781 cttgatgagt taggttcaat ccccaaggga ccaaattttc agttttattt ttgatacgag
    12841 ctatattgtc cttatgacga atgataatca aactagcaag tgctaagata atagcgatga
    12901 agagagagtc atagttactc aggataaaac caaaaagtgg aaagagcaga accccgataa
    12961 cagccgcgat cgatgctgtg acactagaca gtgaaatcat actgccaaga tagagagctc
    13021 caaagaagat aatcgcaagg tagagacaga agataggcgc aaatccgaaa atcactccag
    13081 cactggttgc gacagcctta ccacctttaa atcctgcaaa gatagggaag gtatggccga
    13141 taacagccaa aagtccaaag atgagaggag aaacgccttg tagatgaaaa ataatcggaa
    13201 gcagcgttgc tagggttcct ttgaaaaagt caatcacaaa ggttgccata ccagctttct
    13261 tacctaaaat gcggaaggta ttggtcgttc cagtgttacc agaaccatgc tcgcgtagat
    13321 tgatttgaaa gaatacttgt ccaatccaga gaccagatgg aatcgaaccc agcagatagg
    13381 ctaggattaa taaaattatt gtaatcatac tcctattata tcatgaattg ggaaagaaaa
    13441 gcagagaatc tctgctgaaa ttgtcacatc ggagaaagaa aaattttgca aaatccttgg
    13501 aaaacctgta gaatagtaaa gatgaacgaa taggaggttc cttgtgtcaa aaaaggaaat
    13561 caatattaac aattataatg atgatgctat tcaggtgcta gaagggttgg atgcggtccg
    13621 aaaacgtcca gggatgtata ttggatcgac cgatggcgct ggtcttcatc acctagtttg
    13681 ggaaatcgtt gataatgcag tcgatgaagc cttgtctggg tttggtgatc gtattgatgt
    13741 aactatcaat aaagacggta gtctaacggt tcaagaccat ggacgtggga tgccgacagg
    13801 tatgcacgct atgggaattc caactgttga ggttatcttt accattcttc atgccggagg
    13861 gaaattcggt caaggtggct ataagacatc aggtggactt cacggagtgg gttcttccgt
    13921 tgttaacgcc ctttctagct ggttagaagt tgaaattacc cgtgatggcg cagtttacaa
    13981 gcaacgtttt gaaaatggtg gaaaacctgt cacgactttg aagaaagtcg gtacagcacc
    14041 caagtctaaa acaggcacca aagttacttt tatgcctgac gcgactatct tttctacgac
    14101 agatttcaag tacaatacca tttcagagcg ccttaatgaa tcagcctttc tcttgaaaaa
    14161 tgtgaccttg tctttaacgg acaagcgatc agatgaagcg attgagttcc actatgagaa
    14221 tggagtacaa gattttgttt cttatctcaa cgaagataag gaaatcttga cgccagttct
    14281 ttactttgaa ggggaagaca atggttttca agtggaagta gccctccagt acaatgacgg
    14341 attctcagat aacattctat cctttgtcaa taacgttcgc accaaggacg gtggaacgca
    14401 cgagacagga ctcaagtctg ccattaccaa ggtcatgaat gactatgcac gtaaaacagg
    14461 tcttctcaag gaaaaagata aaaaccttga aggttcagac tatcgtgagg gactagcggc
    14521 cgttctttct atcttagttc ctgaagaaca cttgcagttt gaaggacaga ccaaggataa
    14581 actaggaagc cccttagctc gcccagttgt ggatggaata gtggctgata agttgacctt
    14641 tttccttatg gaaaatgggg aattagcttc taacctcatc cgcaaggcta tcaaggcccg
    14701 tgatgctcgt gaagcagcac gtaaggcgcg tgatgagagc cgaaatggga agaaaaacaa
    14761 gaaagataag ggcttgttgt ctgggaaatt gaccccagcc caatctaaga atcctgctaa
    14821 gaatgaactc tatctagttg agggggactc tgccggtggt tctgccaaac aaggtcgtga
    14881 ccgcaagttc caggctattc tacctcttcg tggtaaggtt atcaatacag ccaaggccaa
    14941 gatggcggat atcctcaaaa atgaagagat caataccatg atttatacca ttggtgcggg
    15001 tgttggagca gacttctcta ttgaagatgc caactatgat aagatcatta tcatgaccga
    15061 tgcggatacc gacggtgccc atatccagac cttgctcttg acatttttct accgttacat
    15121 gcgtccgcta gtcgaggcag gtcatgtcta tattgccctc ccacctcttt acaagatgtc
    15181 caaaggtaaa ggcaagaaag aagaagtggc ctacgcttgg acggacggag aactagaaga
    15241 actacgtaaa cagttcggta aaggcgctac cctccaacga tacaaaggac ttggtgagat
    15301 gaatgcggac cagctctggg aaacaaccat gaacccagaa acacgtaccc tcatccgtgt
    15361 cacaattgaa gatttagcgc gcgccgaacg tcgcgtcaat gttctcatgg gagataaggt
    15421 agaaccacgc cgtaaatgga ttgaagataa tgtcaagttt acgctagaag aagcgacagt
    15481 gttttaatga aagaaaataa tatgttagta aactggaaat tagagtcgga tgtgaatgac
    15541 tatgtgaaaa agcagtttga aaatcttggt ttgaaaaaat tgcagaatta ttaatgaaga
    15601 atcttctatg agtgcttatc tcaaagaggc gtttaaggga gcggctaaga cacaaactaa
    15661 gactaatttt ggaaaaccag attttcatat tgaaaaatac aaacaggata gtttggttac
    15721 ttatcgtaac tgaacacggg acgaattccc gtaaaatatc ttttactgca taaaaaccta
    15781 ctctacattc tttgaaagga gttgaacacg ccctagatat tgtgtgaaaa agataaattc
    15841 tcttgtgagt ttgcttactt caagaatttt ctattttcac ttggtatttt atgggctttg
    15901 tatcttatgt ctaacattca aaacatgtcc ctggaggaca tcatgggaga gcgctttggt
    15961 cgctactcca agtacattat tcaagaccgg gctttgccag atattcgtga tgggttgaag
    16021 ccggttcagc gccgtattct ttattctatg aataaggata gcaatacttt tgacaagagc
    16081 taccgtaagt cggccaagtc agtcgggaac atcatgggga atttccaccc acacggggat
    16141 tcttctatct atgatgccat ggttcgtatg tcacagaact ggaaaaatcg tgagattcta
    16201 gttgaaatgc acggtaataa cggttctatg gacggagatc ctcctgcggc tatgcgttat
    16261 actgaggcac gtttgtctga aattgcaggc taccttcttc aggatatcga gaaaaagaca
    16321 gttccttttg catggaactt tgacgatacg gagaaagaac caacggtctt gccagcagcc
    16381 tttccaaacc tcttggtcaa tggttcgact gggatttcgg ctggttatgc cacagacatt
    16441 cctccccata atttagctga ggtcatagat gctgcagttt acatgattga ccacccaact
    16501 gcaaagattg ataaactcat ggaattcttg cctggaccag acttccctac aggggctatt
    16561 attcaaggtc gtgatgaaat caagaaagct tatgagactg ggaaagggcg cgtggttgtt
    16621 cgttccaaga ctgaaattga aaagctaaaa ggtggtaagg aacaaatcgt tattactgag
    16681 attccttatg aaatcaataa ggccaatcta gtcaagaaaa tcgatgatgt tcgtgttaat
    16741 aacaaggtag ctgggattgc tgaggttcgt gatgagtctg accgtgatgg tcttcgtatc
    16801 gctatcgaac ttaagaaaga cgctaatact gagcttgttc tcaactactt atttaagtac
    16861 accgacctac aaatcaacta caactttaat atggtggcga ttgacaattt cacacctcgt
    16921 caggttggga ttgttccaat cctgtctagc tacatcgctc accgtcgaga agtgattttg
    16981 gcgcgttcac gctttgacaa agaaaaggct gagaaacgtc tccatatcgt cgaaggtttg
    17041 attcgtgtga tttcgatttt ggatgaagtc attgctctta tccgtgcttc tgagaataag
    17101 gcggacgcca aggaaaacct caaagttagc tatgatttta cggaagaaca ggctgaggct
    17161 atcgtaactt tgcaactgta ccgtttgacc aataccgatg tggttgtctt gcaggaagaa
    17221 gaagcagagc ttcgtgagaa gattgctatg ctggcggcta ttattggtga tgaaaggact
    17281 atgtacaatc tcatgaagaa agaacttcgt gaggtcaaga agaaatttgc aactcctcgt
    17341 ttgagttctt tagaagacac tgcgaaagca attgagattg atacagctag tcttatcgct
    17401 gaggaagata cctacgtcag cgtgaccaag gcaggttaca tcaagcgtac cagtccacgt
    17461 tcctttgcgg cttccacctt ggaagaaatt ggcaagcgtg atgatgaccg tttgattttt
    17521 gttcaatctg ccaagacaac ccagcacctc ttgatgttca caagtcttgg aaatgtcatc
    17581 tacagaccaa tccatgagtt ggcagatatt cgttggaagg acatcggaga gcatctgagc
    17641 caaaccatca caaactttga aacgaatgaa gaaatccttt atgtggaagt actggatcag
    17701 tttgacgatg cgacaaccta ctttgcagcg actcgccttg gtcaaatcaa acgggtagag
    17761 cgaaaagaat tcactccatg gcggacctat agatctaagt ctgtcaagta tgctaagctc
    17821 aaagacgata cagatcagat tgtagcagtg gctccgatta aactagatga tgttgtcttg
    17881 gttagtcaaa atggttatgc cctgcgtttc aatatcgaag aggttccggt tgtcggtgct
    17941 aaggcagcag gtgtcaaggc tatgaatttg aaagaagatg atgtcctcca atctggcttt
    18001 atctgtaata cttcgtcctt ctacctcttg acccagcgtg gaagcttgaa acgtgtttcc
    18061 attgaggaaa ttctagcaac cagccgtgcc aaacgaggat tacaggtctt gcgtgagttg
    18121 aaaaacaaac cgcatcgtgt cttcttggca ggagcagttg cagagcaagg atttgttggc
    18181 gatttcttca gtacggaagt ggatgtgaac gaccaaactc tgcttgtcca atccaataaa
    18241 ggaacaatct atgaaagccg attgcaagac ttgaacttgt cagaacgcac tagcaatgga
    18301 agcttcattt ctgacacgat ttcagatgaa gaagtttttg acgcttatct tcaggaagta
    18361 gttactgaag ataaataagt gaaatgaaga atcagttcta agagctggtt tttattggag
    18421 aaattttctg aaaattacaa aatatacttg ctattttaga aaaatagtgt agaatataag
    18481 aaataggttt ttagaataag gagtggaata tgacagtaac gattgattgg gaaaacctcg
    18541 gtttttccta tatgaaatta ccttatcgct atcttgctca tttcaaaaat ggacaatggg
    18601 atcaaggaga gcttacagag gatgcaactt tgcatatttc agagtcttct ccaagtcttc
    18661 actatggaca acaagcattt gaaggtttga aagcttatcg tactaaggat ggcagtgttc
    18721 aactgttccg tcctgatgaa aatgctaaac gcctgcaacg tacatgtgac cgtctcttga
    18781 tgccacaagt tccgacagac atgtttgtag aagcttgtaa agcagttgtc cgtgcgaatg
    18841 aagaatacgt accaccatac ggaataggtg gaaccttata tcttcgccct cttttgattg
    18901 gtgtcggaga tattatcggg gtaaaaccgg cagaagagta cattttcacc atctttgcta
    18961 tgccagttgg aaattacttt aaaggtggtt tggtcccaac caacttcttg attcaggatg
    19021 agtacgaccg tgcagcacca aatggtacag gtgcggctaa ggttggtgga aactatgctg
    19081 caagtctctt accaggaaaa atggccaagt cacgtcattt ctcagatgtt atctatctgg
    19141 acccatcaac tcatacaaag attgaagaag tcggatcagc taacttcttt ggaattacag
    19201 ctgataatga atttgtaaca ccattgagtc catctatctt gccatctatt accaagtatt
    19261 ccttgcttta tttggcagaa caccgcttgg gattaactcc tattgagggt gatgttccaa
    19321 ttgataatct tgaccgtttt gtagaggcag gtgcctgtgg tacagcagcg gttatttctc
    19381 caattggagg tattcaacat ggtgatgatt tccatgtatt ctatagtgaa acagaagtag
    19441 gtcctgtgac gcgtaaatta tataatgaat tgacgggtat tcagtttggc gatattgaag
    19501 cgccagaagg ttggattgta aaagtagatt aaaataaacc gaaggagatt ttttatgaaa
    19561 tagaaaaagt ggctcttaac agcaggagtg gtcctgagca cgtcagctat tttagtggct
    19621 tgtggaaaaa ctgataaaga agccgatgca ccgacaacat tttcttatgt ctatgcagta
    19681 gatccagcat cattgggcta cagtatagcg actcgaacat cgacgacaga cgttattgga
    19741 aatgttattg atggtttgat ggaaaatgat aaatacggca atgttgctcc ttctcaaaaa
    19801 gactatgatt tgaatagtac aggatgggct ccaagctatc aagatccagc gtcttacttg
    19861 aatattatgg atccaaaatc tggttctgcc atgaaacacc ttggtattac gaaaggaaaa
    19921 gataaggatg ttgtagctaa acctggtttg gataaatata agaaattgtt agaagatgct
    19981 gtttctgaga ccactgacct agagaagaga tatgaaaaat atgccaaagc tcaagcttgg
    20041 tcgacagata gttcattatt gatgccaaca gcttcatctg gtggttctcc agttgtaagt
    20101 aacgtagtac cattctcaaa accatactca caagttggta ttaaggggga tccatatatc
    20161 tttaaaggaa tgaaattgca aaaagatatt gttacaacaa aagaatataa cgaggttttt
    20221 aaaaaatggc aaaaagaaaa attggaatcc aatagcaaat accaaaaaga actagaaaaa
    20281 cacattaaat aaggaatggt attgatcttg ataaaatttt aaaaatactg tcattttgaa
    20341 tataaaggag tttgatatgg agtggattag attaatagga atagcaatca ttgttgtggg
    20401 ttttatttta aaatttgata caattgcaac agtagtctta gctggtttgg ttacagcttt
    20461 agtttcaggt gtttctctcg ttgaattttt ggagattttg ggaaaagaat ttagcaatca
    20521 gcgagtgctc acgattttta tggttacctt gcctcttgtg gggctgtcag aaacctttgg
    20581 actcaagcaa cgatcaatcg atttgattcg aaagattaaa ggtctgacag ttggaaactt
    20641 ctatacagtt tatttcttta ttcgagagtt agctggtttc ttttcaattc gtctaggagg
    20701 acaccctcag tttgtcagac ctttggttca gcctatggga gaagcagctg cagagtctca
    20761 attaggtaga aagttaacag aggttgaaga tgagacaata aaagcgcgtg cggcagcgaa
    20821 tgaaaatttt ggaaatttct ttgctcaaaa tacgtttgta ggtgctgggg gagtcctctt
    20881 gataggggga acattagatc agttaggcta tgaaagcaat tatgcaggga ttgcttctac
    20941 atctattatt gttgctgtta taacacttat tgtagtgggg atttacaatt atttatttga
    21001 taaaaaattg atatcaaaaa agactagggg aggagaacaa aaatgacaga gttggcaaag
    21061 caactattag agttgaccta tattgtgatt ggttgtcaat ttctccatac agcctattgt
    21121 agttataaag ataaaacaaa cccagttcga cttgggacat ctgcattttg gactctattg
    21181 tctattacgt ttataggtgg ttcctatatg ccaaatatga gtattggtat tattgtaatc
    21241 ctattatcgc tgttaacatt gtttaagcaa gtccgtatcg gaaccttgcc atccttagat
    21301 gaaatgaaag ccaatattga atctaacagg ttgaaaaata aaatttttat tccagttatg
    21361 ctgatggcaa tacttgcgtt ggtcttagcg caaatgattc cagaatttag caagatttcg
    21421 attagccttg ccgccttgtt tgctacaatt tctgttcttg tgattaccaa tagtcaccct
    21481 aagagtctgt tatcagaaaa taatcgaatg actcagcaag tttcaacaag tggtattgtt
    21541 cctcaattat taggggcttt gggggctatt tttactgtag caggtgttgg tgatgttatc
    21601 tctcatctga ttagcggtat tgttccttca gatagtcgct ttataggagt tttggcctat
    21661 gttcttggaa tggttctatt cacaatgatt atgggaaatg cttttgcagc attcaccgtt
    21721 attacagcag gtgttggagt tccctttgta tttgctctgg gagctaatcc aattgtggct
    21781 ggtgctcttg ccatgacagc aggttattgt gggaccttat tgaccccaat ggctgctaat
    21841 tttaacgctc taccagcagc attgatggat atgaaagatc agaatggcgt tataaaggct
    21901 caagcaggtg ttgctctagt aatgattgtt attcacatat tcttaatgta ctttctcgca
    21961 ttttagtaaa ggaaaataaa atgaaaatat tggttacagg ttttaatcct tttggaggtg
    22021 aaaagattaa tccagctttg gaggctgtaa aattattacc atctgagatt aatggggctg
    22081 aagttcgctg ggtagaaatt ccaacggttt tttataagtc gtcagaagtt ttagaggcag
    22141 aaatattacg atatcaacca gatgctgtac tttgtattgg acaagcaggc ggcaggaccg
    22201 gcttaacacc tgaacgagtg gctattaatc aagatgatgc tcgcatacct gataacgaag
    22261 gcaatcaacc aattgataca ccgattcgta ttgatggagc atcggcctat tttagtagtt
    22321 tacctatcaa agcgatggta caagctatca aaaaacaggg acttccggca gttgtatcca
    22381 atagtgcagg aacctttgtt tgcaatcatt tgatgtacca agctctttat ttagtggata
    22441 aaaaattccc taatatgaga gcaggtttca tgcatattcc atatatgatg gaacaagtag
    22501 taaataagcc gaatactgca ggaatgagtc tatgtgatat tgtaagaggc atagaggtag
    22561 ctattgaagc tattgtagac tacaaagata aggatttgca gttagtaggc ggtgagactc
    22621 actgataacc taaaaaggat agtcaattat gcttgtttac taactattaa ctatgctaaa
    22681 tcaattgagg ttgtttacat aaaactctat atcagagaag cctgatatag agttttttct
    22741 tgctagtttt agaattttct tgtaaaatag aaaaagtgaa gagaggtatg aaatgagcaa
    22801 gaaagataaa aaaatcgaaa ttcaagtagc ggatgccaaa gttaatgttg gtaaagacag
    22861 ttttgaaggt tatacattga ctatcggtaa aaaagttatc ggagaaattg ccgaattaga
    22921 cggacaattt gccattataa agaatgggaa tgtcgatagt ttttataaaa aattggaaaa
    22981 agctgtggaa attttgattg aaaattataa tttagcaaaa taagtcttgt ttttttgaaa
    23041 ttttcatgat ataatagtcc atgttgattg taggagagat agcgaagagg ctaaacgcgg
    23101 cggactgtaa atccgctcct tcgggttcgg gggttcgaat ccctctctct ccatttcatt
    23161 aatggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa
    23221 tccagctacc ccagttctta ggtaataatc aagataaaaa gcaaaatatc ttagggtatt
    23281 ttatttttat aattgaaaga cgtgaatgat atgaacatgt ccttgcgggt gcttaggaaa
    23341 aaaattataa gtatgtcaag tttaagaaaa acttgattgt tggaggattt tttagatgaa
    23401 cgaatttgaa gatttgctaa atagcgttag tcaagttgag actggtgatg ttgttagtgc
    23461 tgaagtattg acagttgatg cgactcaagc taacgttgca atctctggaa ctggtgttga
    23521 aggtgtcttg actcttcgcg aattgacaaa cgatcgtgat gcagatatca atgactttgt
    23581 taaagtagga gaagtattgg atgttcttgt acttcgtcaa gtagttggta aagatactga
    23641 tacagttaca taccttgtat ctaaaaaacg ccttgaagct cgcaaagcat gggacaaact
    23701 tgttggtcgc gaagaagaag ttgttactgt taaaggaacg cgtgccgtta aaggtggact
    23761 ttcagtagaa tttgaaggtg ttcgtggatt tatcccagct tcaatgttgg atactcgttt
    23821 cgtacgtaat gctgagcgtt ttgtaggtca agaatttgat actaaaatca aagaagttaa
    23881 cgctaaagaa aaccgcttca tcctttcacg tcgtgaagtt gttgaagcag ctactgcagc
    23941 agctcgcgct gaagtattcg gtaaattggc tgttggtgat gttgtaactg gtaaagttgc
    24001 tcgtatcaca agcttcggcg ctttcgtcga ccttggtggt gttgacggat tggttcactt
    24061 gactgaattg tcacatgaac gtaacgtatc accaaaatca gttgtaactg ttggtgaaga
    24121 aattgaagtg aaaatccttg atcttaacga agaagaagga cgtgtatcac tttcacttaa
    24181 agcaacagta ccaggaccat gggatggcgt tgagcaaaaa ttggctaaag gtgatgtagt
    24241 agaaggaaca gttaaacgtt tgactgactt cggtgcattt gttgaagtat tgccaggtat
    24301 cgatggactt gttcacgtat cacaaatttc acacaaacgg attgaaaatc caaaagaagc
    24361 tcttaaagtt ggtcaagaag ttcaagttaa agttcttgaa gttaacgcag atgcagaacg
    24421 cgtgtcactt tctattaaag ctcttgaaga acgtccagcc caagaagaag gacaaaaaga
    24481 agaaaaacgt gctgctcgtc cacgtcgtcc aagacgtcaa gaaaagcgtg atttcgaact
    24541 tccagaaaca caaacaggat tttcaatggc tgatttgttt ggtgatatcg aactttaatc
    24601 aaattgaaaa ttcacaaaat cctttgttta ctaaacaagg gatttttct
//
LOCUS       DAVKLW010000029        24418 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKLW010000029 DAVKLW010000000
VERSION     DAVKLW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.029"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            633..1910
                     /gene="ltrA"
                     /locus_tag="VW324_001174"
     CDS             633..1910
                     /gene="ltrA"
                     /locus_tag="VW324_001174"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9726819.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            2366..3544
                     /locus_tag="VW324_001175"
     CDS             2366..3544
                     /locus_tag="VW324_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9726820.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3858..3989
                     /locus_tag="VW324_001176"
     CDS             3858..3989
                     /locus_tag="VW324_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9726821.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4010..5560
                     /gene="dltA"
                     /locus_tag="VW324_001177"
     CDS             4010..5560
                     /gene="dltA"
                     /locus_tag="VW324_001177"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9726822.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVFTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5557..6801
                     /gene="dltB"
                     /locus_tag="VW324_001178"
     CDS             5557..6801
                     /gene="dltB"
                     /locus_tag="VW324_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9726823.1"
                     /translation="MMEFFQQLPHLEPYGKPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6815..7054
                     /gene="dltC"
                     /locus_tag="VW324_001179"
     CDS             6815..7054
                     /gene="dltC"
                     /locus_tag="VW324_001179"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9726824.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7047..8315
                     /gene="dltD"
                     /locus_tag="VW324_001180"
     CDS             7047..8315
                     /gene="dltD"
                     /locus_tag="VW324_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9726825.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8470..8910
                     /gene="adcR"
                     /locus_tag="VW324_001181"
     CDS             8470..8910
                     /gene="adcR"
                     /locus_tag="VW324_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9726826.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8910..9614
                     /locus_tag="VW324_001182"
     CDS             8910..9614
                     /locus_tag="VW324_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9726827.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            9607..10413
                     /locus_tag="VW324_001183"
     CDS             9607..10413
                     /locus_tag="VW324_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9726828.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10423..11928
                     /gene="adcA"
                     /locus_tag="VW324_001184"
     CDS             10423..11928
                     /gene="adcA"
                     /locus_tag="VW324_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9726829.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13098..13871)
                     /locus_tag="VW324_001185"
     CDS             complement(13098..13871)
                     /locus_tag="VW324_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9726830.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            14047..15450
                     /locus_tag="VW324_001186"
     CDS             14047..15450
                     /locus_tag="VW324_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9726831.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLVDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            15565..16203
                     /locus_tag="VW324_001187"
     CDS             15565..16203
                     /locus_tag="VW324_001187"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9726832.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            16215..16655
                     /locus_tag="VW324_001188"
     CDS             16215..16655
                     /locus_tag="VW324_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9726833.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            16648..17079
                     /locus_tag="VW324_001189"
     CDS             16648..17079
                     /locus_tag="VW324_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9726834.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            17070..17540
                     /locus_tag="VW324_001190"
     CDS             17070..17540
                     /locus_tag="VW324_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9726835.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            17565..18338
                     /locus_tag="VW324_001191"
     CDS             17565..18338
                     /locus_tag="VW324_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9726836.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            18335..19135
                     /locus_tag="VW324_001192"
     CDS             18335..19135
                     /locus_tag="VW324_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9726837.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            19194..21488
                     /locus_tag="VW324_001193"
     CDS             19194..21488
                     /locus_tag="VW324_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9726838.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            21498..>24418
                     /locus_tag="VW324_001194"
     CDS             21498..>24418
                     /locus_tag="VW324_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9726839.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     I"
BASE COUNT         7690 a         3968 c         5186 g         7574 t
ORIGIN      
        1 gtagtacgac gggcatgtcg tatatctgag gtgtaagtcc tcggtgggca cctgctaccg
       61 gtgaacccaa tagcgattcc caagcctgac tatcgtgagg tagcagggag aggaagggat
      121 agcgaaatcg tggctctacg aacaggaacg tgatagtaag gcgtatatag cggataagga
      181 ggcttcaaac tctaaagtcc aaaaaggtag tcgtaaccta tatgtgtaaa tcacgagagt
      241 aattgaattc ggactaaggt ttgtgtgaaa aagataaatc tttctagagt ctaaagactc
      301 tgcgtcagat ttcctatttt cactgtaacc ttttaacgtc ctcatatctt gtataaacga
      361 ggaaagatgt acgacttatc ccgtgaggtt tcatgagcgc tgaaagcgta gtaacaacga
      421 atcatgagaa gtcagccgag cccatagtag tgaggaaact tccgtaatgg aagtggagcg
      481 aaggggtgaa caactaatcg aagtaatcct acttcacttg tgtctgtaaa atgagtggtc
      541 tgatagaact ggactttgac acgtaatgac tagatgaagg ttcaccaata taagatgtcc
      601 ctcaggcacc aaaacaagaa aggaatacgc acatgtcaaa actgctagat aagatattat
      661 cacgcgaaaa tatgctggaa gcctacaatc aagtaaaatc caataaaggc tcagctggga
      721 ttgatggaat gactatcgaa gagatggata attatctcag acaaaactgg cgcttgacta
      781 aggaactgat aaaacagaga aaatataagc ctcaaccagt tcttaaagtt gagataccta
      841 aaccagacgg aggcatccgt caactaggaa ttccaacagt tatggataga atgattcaac
      901 aggccattgt ccaagtcatg agccccattt gtgaacccca tttctcagat acgagttatg
      961 gtttcagacc aaataggtca tgtgaaaaag ccatcatgaa gctcttagaa tacttaaatg
     1021 acggctatga gtggatagtg gatatagacc tagagaaatt tttcgataca gttcctcaag
     1081 atagattgat gtccttagta cataacatta tcgaagacgg agatacggaa tccttgattc
     1141 gtaagtatct tcattcaggt gttatcatta atggtcaacg ttataaaaca ctagttggta
     1201 caccacaggg aggaaattta tctcctctct tatccaatat catgcttaat gaattggaca
     1261 aggaattaga aaagagggga cttcgatttg tgcgctacgc agatgattgt gtgattacgg
     1321 tcggaagcga ggcagcctct aagcgtgtga tgtattcagt cagtcgtttt attgagaaac
     1381 ggctaggttt gaaagtaaac atgaccaaga ctaagattac cagaccaaga gagttgaaat
     1441 atctaggttt tgggttctgg aaatcatcag atggttggaa aagccgtcca catcaagata
     1501 gtgttcggag atttaagttt aaattgaaga aactaacaca gaggaaatgg agtatagacc
     1561 taacaagacg tattgagcaa ctgaatttgt ctattcgagg atggataaac tatttctcat
     1621 tgggaaatat gaaaagtata gtcgccagca tagatgagcg cttgcgtact cgcctacgag
     1681 tgattatctg gaagcaatgg aagaagaaat cgagacgatt atggggattg cttaagttag
     1741 gagttcctaa atggatagca gataaggtat ctggctgggg cgaccattat caattagtag
     1801 ctcagaagtc ggtacttaaa cgtgctatat caaaaccagt cctggaaaaa cgtggactgg
     1861 tttcgtgttt ggattattac cttgaacgac atgcgttaaa agttagttga accgccgtat
     1921 gccgaacggc acgtacggtg gtgtgagagg ggctagagat tatcccctac tcgattaact
     1981 cccctgaaat ttattttaat tatgcaaatt tcacgtattt ttgatgctga gacgacgatc
     2041 ctgggaactt ttcagatatt tttttgacta tctaaatcta tcattagaaa agcttagagc
     2101 gccaaaggat ttgagcgttt ttctgatttt taagactttt tccagtctct ttttcgattg
     2161 aagatgtaat tattctacta actaactaac ttcttagtac tagccaacaa cgataatcat
     2221 aattcctcct aaaattagga ataataaagg caatagtttt tgttttttca tgtaaaaaac
     2281 ctcacttttg ttttctgcta ttttatgcta aaatattaaa aatcaaattt aattccaaag
     2341 tttgtaacta aagggggagc gctacatgtc taattcattt gtcaagttgt tagtctctca
     2401 attatttgca aatttagcag atattttctt tagagtaaca atcattgcta acatatacat
     2461 tatttcaaaa tcagtaattg ccacatcact agttcctatc ttaataggaa tatcctcttt
     2521 tgttgcgagt cttttagttc cgttggttac taaaaggtta gcgctaaata gggttttatc
     2581 tttatctcaa tttggaaaga ctatattatt ggcgatactg gtaggaatgt ttaccgtaat
     2641 gcaatccgta gcgcctttgg tgacctatct atttgttgtt gcaatttcca tactagatgg
     2701 ttttgcagca cccgtttcct atgctattgt gccacgctat gcgaccgatt tgggtaaggc
     2761 taattcagcc ttatcaatga ctggtgaagc tgttcaattg ataggttggg gattaggtgg
     2821 actcttgttt gcaacaattg gtctgttacc taccacgttt atcattttag tcttgtatat
     2881 catttctagc tttctgatgt tatttcttcc taacgctgaa gtggaggtgt tagagtcaga
     2941 aactaatctt gaaattttgc tcaaaggttg gaagttagtt gctagaaatc ctagattaag
     3001 actttttgta tcagcaaatt tattggaaat tttttcaaat acgatttggg tttcttccat
     3061 tatacttgtt tttgtaacgg agttattaaa taaaacggaa agttactggg gatattctaa
     3121 tacagcatac tctattggta ttataattag tggcttaatt gcttttaggc tatctgaaaa
     3181 gttccttgct gctaaatggg aaagtattct ttttcctctt gtagctatgg caatagtgac
     3241 actgactatt ctatattttc caaacgcaca gatgttttta ttattttcag ctttagttgg
     3301 tatgttatcg caactaaaag aagttcctga aagtgtattt cttcaggaaa cagtagagga
     3361 aaatcattta gttaatgtct attccgttct tgaagtgatt tctacattag cattttcagt
     3421 atttgttttg ctaatgagct atattactga gagttttggt attagcatca gtttttggct
     3481 atcagccatt tgtctgatga tagaagctat tttaatttat atcagacgag attattttaa
     3541 atgagagagt ggttaaaaag tctttaaaaa tcagaaaagc gcatagtatc aggtgttgaa
     3601 aaaacttgat attgtgtgtt ttattatggg aatatttact tcattgtctc ctgaaatgga
     3661 gttttgaagc aggatcactc tatttgaaaa tatacataga aaaactgtat aatccttaaa
     3721 ataatctgaa atctattgga aacatacggt aaacaattga aaagatacta tataaagtgg
     3781 tacaatggta gaaaaataag ctagtaagaa tcactcttat tttagcgaaa aattacagaa
     3841 atgaatggtt tttcttgatg aaacagagaa aagaattgta cctctttctt ggtcggacag
     3901 ccttgtattt tcttatcttt ctagggctgc tttacttctt tagctatctt ggtcagggtc
     3961 aaggaagctt tatctataat gaattttaac ttgaaggaga atccctattg tgtcaaataa
     4021 accaatagca gatatgattg aaaccattga gcattttgct cagacacagc ctagctatcc
     4081 tgtctataat gttttggggc aggaacacac ttatggcgat ttaaaggctg attcggatag
     4141 tttggctgca gtcattgacc aactaggctt gcctgagaag tctcctgtgg ttgtttttgg
     4201 tggccaagaa tatgaaatgt tggcaacctt tgtagcgctg actaagtcag gtcatgccta
     4261 cattccaatt gatagccatt cggccttgga gcgagtttca gctattttag aagtagcaga
     4321 gccaagcttg attattgcca tttcagcctt tcccttggag caggttttta caccaatgat
     4381 aaatctagct caggttcaag aagcctttgc ccaagggaat aactatgaaa tcacgcatcc
     4441 agtcaaggga gatgataatt actacattat ctttacttct ggtacgactg gtaagcctaa
     4501 gggagtgcag atttcacatg ataatctcct cagctttaca aactggatga ttacggataa
     4561 ggaatttgcg acaccgagtc gtccgcaaat gctggcacag ccaccttatt cttttgactt
     4621 gtctgtcatg tattgggcac cgaccttggc acttggtggt acgcttttca ctcttccttc
     4681 agtcatcact caggacttta agcaactctt tgcggctatc ttttcattgc caatcgctat
     4741 ctggacatca acaccatcct ttgcagatat ggccatgttg tctgaatact tcaacagtga
     4801 gaaaatgcct ggaatcacgc atttctactt tgatggtgaa gaattgacgg tcaaaacagc
     4861 tcaaaaactg cgcgagcgtt tcccaaatgc ccgtatcatc aatgcttacg gcccaacaga
     4921 agcgacagta gctctgtcag cagttgccgt gacagacgag atgttagcga ctctcaaacg
     4981 cctaccaatc ggctatacca aggctgattc tccaaccttt atcattgacg aggaaggaaa
     5041 taaactgcca aatggtgagc agggagaaat cattgtttct gggccagctg tttcaaaagg
     5101 ttatatgaac aatcctgaaa aaacagcaga agccttcttt gagtttgaag atctgccagc
     5161 ctatcacaca ggcgatgtgg gaaccatgac agatgagggc ttgcttctct acggcggacg
     5221 catggacttc cagattaagt ttaacggtta ccgcattgag ttagaagatg tctctcaaaa
     5281 cctcaacaag tctcgcttta tcgaatctgc tgtcgcagta ccgcgctata acaaggacca
     5341 caaggtacaa aatctattgg cttatgtcat cttaaaagac ggtgttcgtg agcagtttga
     5401 gcgagatatc gatattacca aggccatcaa ggaagacctg acagacatca tgatgtccta
     5461 tatgatgcca tctaaattcc tttaccgaga cagtttgcca ctaactccaa atggaaagat
     5521 tgacatcaaa ggattgatta acgaggtgaa taagagatga tggagttttt tcaacagctt
     5581 cctcatttag agccatatgg caaacctcag tattttgttt atgtgattgc tgcaaccttg
     5641 cccatcttta taggtctctt tttcaagaaa cgctttgcct ggtatgaagt gttggtaagt
     5701 ctcttcttta ttgtcaccat gttggtgggt ggaaagacca atcaactagc tgccttgggt
     5761 atttaccttt gctgggaaat attgctcctg cttttctaca agcattatcg aaaaagcaag
     5821 gatggcaagt gggtcttcta cttagttagt tttctgtccc tacttccgat tatctttgtc
     5881 aaggtgcaac cagctatcaa tggaacgcag tctttgcttg ggttcttggg aatttcttac
     5941 ctgacctttc gttcggttgg aattgtcatc gagctgagag atggagtgat taaggatttt
     6001 accctctggg aattcctccg tttccttctc ttcatgccaa ctttctcgag tggtccaatc
     6061 gatcgcttta agcgatttaa tgaaaattat caggctattc ctgagcgaga tgagttgatg
     6121 gatatgctgg atgaatctgt ccgctatatc atgtggggct ttttgtataa gtttatccta
     6181 gctcatgttt taggagagac cttactacct cctctgaaga atttagcctt gcagtcaggt
     6241 ggcttcttta atctctatgc cttggcagtt atgtatactt ttggtctgga gctcttcttt
     6301 gactttgcag gttattctat gtttgctttg gccatctcaa acttgatggg aatccgtagc
     6361 cctatcaact ttaacaagcc ctttttatca agggatttaa aggagttttg gaatcgctgg
     6421 catatgagtc tgtccttctg gttccgtgac tttgtcttta tgcgaatggt gatggtgtta
     6481 accagaaaga aagtctttaa aaatcgtaat gtaacctcaa gcatggccta cattgtaaat
     6541 atgctgatta tgggattttg gcatggcgtg acctggtact atatcgccta tggactcttt
     6601 catggactag gcttggtcat caatgatgcc tgggttcgca agaaaaaaac gctcaataag
     6661 gaacggaaaa aagcagggaa ggctgcccta cctgagaatc gctggattca gtttcttggc
     6721 atggttgtca ctttccatgt tgtcatgttg tcattcttaa tcttttctgg attcttgaat
     6781 aatctatggt ttaaaaaata aaaggaaata aaatatggat atcaaatcag aagttattga
     6841 aattattgat gagttgttta tggaagatgt ttctgacatg atggatgaag atctttttga
     6901 tgcaggtgtc ttggatagta tgggaacggt tgagttgatt gtggagattg agaaccgttt
     6961 tgacattcgt gtccctgtaa cagagtttgg tcgtgacgac tggaatacag ctaataaaat
     7021 catagctggt attgtggagc tacaaaatgc ttaaacgctt atggatgatc ttcggaccgg
     7081 tcttgatcgc tggtttgttg gtttttctgc tcattttctt ttatcctact gagatgcatc
     7141 ataatctagg agctgaaaag cgttcagcag tggctactac tatcgatagt tttaaggagc
     7201 gaagtcaaaa agtcagagca ctatctgatc caaatgtgcg ttttgttccc ttctttggct
     7261 ctagtgaatg gcttcgtttt gacggtgctc atcctgcggt attagctgag aaatacaatc
     7321 gttcctaccg tccttatctt ttaggacagg ggggagctgc atcgcttaac caatattttg
     7381 gaatgcaaca gatgttacca cagctggaga ataaacaagt tgtgtatgtt atctcacctc
     7441 agtggttcag taaaaatggc tatgatccag cagccttcca gcagtatttt aatggagacc
     7501 agttgactag ttttctgaaa catcaatctg gggatcaggc tagtcaatat gcagcgactc
     7561 gcttactgca acagttccca aacgtagcta tgaaggacct ggttcagaag ttggcaagta
     7621 aaggagaatt gtcgacagca gacaatgaaa tgattgaatt attggctcgt tttaatgaac
     7681 gccaagcctc cttttttggt cagttttcgg ttagaggcta tgttaactac gataagcatg
     7741 tagctaagta tttaaaaatc ttgccagacc agttttctta tcaagcaata gaagatgttg
     7801 tcaaagcaga tgctgaaaaa aatacttcca ataatgagat gggaatggaa aattatttct
     7861 ataatgagca gatcaagaag gatttgaaga aattaaagga ttctcagaaa agctttacct
     7921 atctcaagtc gccagagtat aatgacttgc agttggtttt aacacagttt tctaaatcta
     7981 aggtaaaccc gatttttatc attccacctg ttaataaaaa atggatggac tatgctggtc
     8041 tacgagagga tatgtaccaa caaacggtgc agaagattcg ctaccagtta gaaagtcaag
     8101 gttttaccaa tatagcagat ttttctaagg acggcgggga gcctttcttt atgaaggaca
     8161 ccattcacct tggttggttg ggttggttgg cttttgacaa ggcagttgat cctttcctat
     8221 ccaatcccac accagctccg acttaccatc tgaatgagcg ctttttcagc aaagattggg
     8281 cgacttatga tggagatgtc aaagaatttc aatagataat ataatataga agagttcaag
     8341 aatgaaacta gttttcacgt tttttcttga ctcttttttt gcttgtgata taattaactg
     8401 gtaaacaaaa tgtcaaagaa tagtattttt ctcttaaaaa agagaagtct aaaaggaaag
     8461 gagtcagata tgagacagct agcaaaggat atcaatgctt ttttgaatga ggtgattttg
     8521 caggcggaaa atcagcatga aatcctaata ggtcattgca ctagcgaggt ggccctgacc
     8581 aatactcagg agcatattct tatgctcttg tcagaggaat ctttaacaaa ttcagaattg
     8641 gcccgtcgtc tcaatgtcag tcaggcggca gttaccaagg ccattaagtc tttggtcaag
     8701 gaagggatgt tggaaacatc taaagattct aaagatgcgc gtgtgatttt ttatcagttg
     8761 actgacttgg ctcgtccaat cgctgaggag caccaccatc accatgagca tacactttta
     8821 acctatgaac aagtggcgac tcagtttact ccaaatgaac aaaaagtgat tcagcggttt
     8881 ttgactgctt tagtaggaga aatcaaataa tgagatatat tacggtagag gatttgtcct
     8941 tctattatga taaggagcct gttcttgaac atatcaatta tagtgttgat agtggggaat
     9001 ttgttacctt gactggggaa aatggagcgg ctaagacgac gctcatcaag gccagtcttg
     9061 gaattctgca accacgcatt ggaaaggtgg ctatttcaaa gacaaatacg caaggtaaga
     9121 aattgagaat agcctatctt cctcaacaaa ttgccagttt taatgctggt tttccaagta
     9181 cggtctatga atttgtcaag tcgggtcgct atccgagaaa aggctggttc cgtcgtttga
     9241 atgctcatga tgaggagcat atcaaggcta gtctggactc agttggcatg tgggaacatc
     9301 gagacaaacg cttggggtct ctatctgggg gacaaaagca gcgagcggtg attgcgcgta
     9361 tgtttgcttc tgaccctgat gtgtttatcc tagacgagcc gacaacgggg atggatgcag
     9421 gaagtaaaaa tgaattttac gaactcatgc accacagcgc ccatcatcat ggcaaggctg
     9481 ttttgatgat tacccatgac cctgaagaag ttaaggatta tgcggatcgc aatattcatc
     9541 tagtccgtaa ccaagactcg ccatggcgtt gtttcaatgt tcatgagaat ggccaggagg
     9601 tgggccatgc ttagtttatt atcttacgac tttatacaac gcgccttttt ggcggttatt
     9661 gctatgagtc ttttctcacc ggtattggga accttcctca tcttgcgtcg tcagagtttg
     9721 atgagtgata cccttagcca cgtctcactt tcaggtgtag cctttggtct ggttttgggg
     9781 atttctccaa ctgtttctac tattgccatt gtcttgattg cggcggtctt tctggagtat
     9841 ctccgtacgg tttacaagag ctttatggaa atcgggacag ctatcctcat gtcaacaggt
     9901 ctggctgttt ctctgattgt catgagcaag ggtaaaagct cgagttcaat gagtttggac
     9961 caatatctct ttggttcgat cgtgactatc agtgaagaac aggtcatttc cctctttgtc
    10021 attgcggcgg ttgttttgat tttgaccttt ctctttcttc gtcctatgta tatcttaact
    10081 tttgacgaag atacggcctt tgtggatggc ttgccagttc gtaccatgtc cattcttttt
    10141 aacatggtga caggggtggc tattgccctt atgattcctg cagcaggagc tcttctggta
    10201 tcgaccatta tggtcttgcc agctagtatt gccctgcgtc tggggaaaaa ctttaaatcg
    10261 gttatgctgc ttgccagtgc gattggcttt ttgggaatgg tagcaggact ttacatttcc
    10321 tactatgcag aaacacctgc aagtgcaagt attaccatta tttttgtaac tgtctttata
    10381 ctaatcagtt tagtaagacg ttttatcaaa taggagacga acgtgaaaaa aattagctta
    10441 ttactagcca gtctatgtgc cttgttttta gtggcttgtt ccaatcaaaa acaggcagat
    10501 ggtaaactca atatcgtgac aaccttttac cctgtctatg aatttaccaa gcaagttgca
    10561 ggagatacgg ctaatgtaga actcctaatc ggtgctggga cagaacctca tgaatacgaa
    10621 ccatctgcca aggcagttgc caaaatccaa gatgcagata ccttcgttta tgaaaatgaa
    10681 aacatggaaa catgggtacc taaattgcta gataccttgg ataagaaaaa agtgaaaacc
    10741 atcaaggcga caggcgatat gttgctcttg ccaggtggcg aggaagaaga gggagaccat
    10801 gaccatggag aagaaggtca tcaccatgag tttgaccccc atgtttggtt atcaccagtt
    10861 cgtgccatta aactagtaga gcacattcgt gacagcttgt cagcagatta tcctgataaa
    10921 aaagagacct ttgagaagaa tgcagctgcc tatatcgaaa aattgcaagc cttggataag
    10981 gcttacgcag aaggcttgtc tcaagcaaaa caaaagagct ttgtgactca acacgcagcc
    11041 tttaactatc ttgccttgga ctatggactc aaacaagtcg caatctcagg cctttctcca
    11101 gatgcagagc catcagctgc tcgcttggca gaattgacag agtatgtcaa gaaaaataaa
    11161 atcgcctata tctattttga agaaaatgcc tcacaagccc ttgctaacac actttcaaaa
    11221 gaagcaggtg tcaaaactga tgtcctcaat cctttagaaa gtctgacaga agaggacacc
    11281 aaggctggag aaaactacat ttccgtgatg gagaaaaacc tcaaggcttt gaaacaaaca
    11341 acagaccaag aaggcccagc aatcgaacct gaaaaggcag aggataccaa gacagtccaa
    11401 aatggttact tcgaggatgc agctgtcaag gaccgcacct tgagtgacta tgcaggtaac
    11461 tggcaatcag tttatccttt ccttgaagac ggcacgtttg accaagtctt tgactacaag
    11521 gctaagttga ctggtaagat gacccaggct gagtacaagg cttactatac aaaaggctat
    11581 cagacagatg tgactaagat taacattact gataatacta tggaatttgt tcaaggtgga
    11641 caaagcaaga aatacactta caagtatgtc ggtaagaaaa ttttgactta caagaaaggc
    11701 aatcgtggcg tgcgtttcct ctttgaagcc acagatgctg acgctggaca attcaagtat
    11761 gttcagttta gtgaccacaa tatcgcccca gttaaggcag aacatttcca tatctttttt
    11821 ggaggtacaa gccaagaaac tctatttgaa gaaatggata attggccaac ctactatcca
    11881 gataacctat ctggccaaga aatcgcccaa gaaatgttgg cgcattgatg agaaatcgac
    11941 tagttcataa gagctagtcg gttttttgat actagatggc taacttcatt atagaccttt
    12001 cagaactaag ggaacctgaa aaaataaata aaactcttga aatatctggg cttatatgct
    12061 agaatgtagt tagtgtataa ggttcattag aacaccaaag cccctaacta gtgccatagt
    12121 taagggcttt tttgacgtat cttagcgatt taacgggtgt ttataggcta gtcattcggt
    12181 ctacttctta ccatctagcc atttgcagac gaaatacaag ataattccag ctataacatc
    12241 tgctataata gtaagagcaa gctctggtat aaggctcatt agtttcacct cctctcacga
    12301 acccatagga acgtaatcgg taaccgatga caaaaatagt ataccacact aaatttagat
    12361 catcaacctc acttaatgct tgaactattg tagattatat gctaaaatgt agttaattga
    12421 taaggttcat tagaacacca aagcccctaa ctatggccgt agttaagggc ttttttgacg
    12481 tatcttagag atttaacggg tgtttatagg ctagtcagtc ggtctactcc ttactatcta
    12541 gccatctgca gacgaaatac aagataattc cagctataac atccgctata atagtaagag
    12601 cgagctctat gataaggctc attagtttca cctcctctca cgaacccata ggaacgtaat
    12661 cggtaaccga tgacaaaaat agtataccac aatacattta gatcatcaag gtcacttaat
    12721 tcttgaaata tagggtctag gcgacaaagt ctttaaaatc agaaaaacgc ataatatcag
    12781 gtgtgcaaaa acttgatact atgcgtttta ttgtgggaag gtttactcca ttttctcctg
    12841 aaattgagtt tttgtccagc ctctgttttt ggggttgcta agaaaataat gtcatgtggt
    12901 gaatatttgt aaatcagtca gcagacagaa cgatactctt cgaaaatctc ttcacatcat
    12961 gtcagcttcg tctttccgta tatatgtgac tgacttcatc agttctatct acaacctcaa
    13021 aacagtgttt cgagctgact tgatcaattt tcaaatctgt actgtgagca agctgagact
    13081 agcttcctat ttgattttca ttgaatatca gaaacccatt ctccatcaaa taattcgact
    13141 gcgtctaata atttttgatc tggcacggtg tctgaaataa aggttgtgta tttggagagg
    13201 ggattaattt taaaaaatcc agtcttgtaa aatttagaac tatcaatcag taagatggtt
    13261 tcatgggctt tgtcaataat attctttttt gaaatagctt ggctgagaga agcttcataa
    13321 acatattggt catcaatacc tcttgctgaa caaaatgcta aatcgatatt aaaatgatct
    13381 aataaagaat tttccttatc gtaattgacc acggaacagg attgatgttt gacctcgcca
    13441 gatgtgataa agattttgga gctatcttta acagtttcag atagggtttg tgcagtatgt
    13501 aaaccatttg taaaaataat caaattatca agttcagaaa gatagggaca aagttcgtag
    13561 acagtcgtac tggaatccag ataaacacac atacctgacc gaataaagtc tttagcgaga
    13621 ctagcgatta gtcttttttg cctagtactt tctccttccc gtatttgatg agaaagttca
    13681 attgtgttca tagaggacag ggtcacgtat ccgtgctttc ttttgataag accttgattt
    13741 tctaagaaaa ttaaatcacg acgtaaggta cttgtgctgg agaaagtgat ttctgccagc
    13801 tcttttacgg caattctttt tttcttttta ataatttcaa tcaattcaag tacacgttca
    13861 tcttttatca taagctcctc ctaatttatc atttcaacta tattatagca caaattggag
    13921 gaatttgaat tatttttatg aatattgggt taacatttga acattattca agtaagcgtt
    13981 cacatattga aaaaataaaa cgtggggatt ataataaagt taatcaagga cgaagagaga
    14041 agaaaaatgg aagcggtttt agcaatagat ttaggtgcga cttctggaag agcaatcgtt
    14101 ggttaccttt ctgaaaataa actagtaatg gaagaaataa atcgcttttc taatctacct
    14161 attagagtaa aagggcattt atcttgggat attgactttc tactagctaa aattcttgaa
    14221 agtatccgct tggctaatac tagttacaag attttatcta tcggtattga cacatgggga
    14281 gttgattttg gactggttga taatgaaggt aagctgttat tacaacctgt tcattatcgt
    14341 gatgaaagaa caaagggagt gttaaaggaa atatctgaaa tgactgaatt agaaaaactg
    14401 tattcagaga caggaaatca gattatggag ataaatacct tgtttcaact ctttaaggca
    14461 cgtcaagaat ctcctgactc tttctataag accaataaga ttcttttaat gccagatttg
    14521 tttaattatc tcttgacagg taagtttgct acagaaaaaa gcattgcttc aacaactcaa
    14581 ttatttgatc ctaggagtca aaactggaat cagaatatct taaaactatt tgaattggat
    14641 tcatctttac ttcctgagat tgtttcagag ggaaatgttc ttggaaggat aaaaaaggag
    14701 tatggtttag gcgatattcc tgttgtgaat gtttgtagtc atgatacagc aagcgcgatt
    14761 gtctcagtac ctaagacaga aggtagttta tttatttcat caggtacttg gtctttggtt
    14821 ggagtggaac ttacttcacc gcttcttact accgaatcct tcagttatgg atttacaaat
    14881 gaagtcggta aagatggagt gattacattt ctgaagaatt gtacagggtt gtggatcata
    14941 gaggaactaa gacgttcatt tgaacgaaga gggaaagcct attcttttga tgatattagg
    15001 acaatggtgg agaaagaaaa agaaaatctt cctctgattg atactgaatc aactgaattt
    15061 gcaacagaat ctgatatgca caagactttg acagaatatc tagcttatca tcatgaaact
    15121 agagagtgga cagatggaca actatttaag attgtttatg aaagcctagc tgaaacgtat
    15181 aggaaagcga tagagttact agaagaacta actcataagg tttataagag gatatatgtg
    15241 attggaggag gtgctagagc cagttacttt aaccaaatga ttgctgatag aactggtaaa
    15301 gaggttctta caggtttgac tgaggctaca gctgtgggga atattgttgt gcagctcata
    15361 gctatgggac aattaaaagg gatggaagaa gctcaccatg ttattgagga gtttctacaa
    15421 ttagagagtt attactccca aaagaattaa aaagattgag agtttgtaaa tttgcctccc
    15481 tcccccttct taagttttgt acaagaaggg gggataattg gtgaattgaa aaatatttag
    15541 tgttttgata tgaggaggac aagaatgtca gatgtaaaac aagaattaat taagtatggc
    15601 aagaagctag tagaaacaga tttgacgaaa ggaacaggtg ggaatctcag tgttttcgat
    15661 cgtgaaaaac aattgatggc aattaccccg tcgggtattg atttctttga aatcaaagaa
    15721 tccgatattg tagtgatgga tattaatgga aatgttgtag agggagaacg cttgccatct
    15781 agcgaatggt atatgcattt gattcaatat caaactcgtg atgatatcga tgcaattatc
    15841 catgctcata caacttatgc aacagtatta gcttgtctca gagaaccact tccggctagt
    15901 cattatatga ttgcagtggc agggaaagat gttcgggtag ctgagtatgc aacatatggt
    15961 acgaaagaat tggctgtgaa tgcagctaaa gcaatggaag gtcgtagagc agttttacta
    16021 gcgaatcatg gaattttagc aggtgcacaa aatttattga atgcatttaa tattgttgaa
    16081 gaagttgaat attgtgcaaa aatttattgt ttagctaaga attttggaga gccagtagtt
    16141 cttcctgatg aggagatgga tttaatggca gaaaaattta aaacatacgg tcagagaaaa
    16201 tagggaggat attaatgtta aaacatatac cgaaaaatat ttctccagat ttattgaaga
    16261 ctttaatgga aatgggacat ggagatgaaa tagtattagc tgacgcgaat tatccttctg
    16321 cctcatgtgc aaataagcta attcgttgtg atggcgtaaa tattccagaa ttattagatt
    16381 ccattctgta tttaatgcca ttagatagtt acgtcgatag ttcaattcag tttatgaacg
    16441 ttgtttcggg tgatgatatt cctaagatat ggggtaccta tagacagatg attgaaggtc
    16501 atggtacaga tcttaaaacg attacttatc ttagaagaga agacttttat gaacgtagta
    16561 agaaagctta tgctattgtt gctacaggag aaacttcact ttatgctaat attatcctta
    16621 agaaaggagt agttgttgaa agagaaaatg tttaatagag gaattttagt tgccagtcat
    16681 ggtaattttg ctagcggagc tctcatgacc gcagaaatgt ttgttggtga gacaacaaat
    16741 gatagagtta ggacattagg tttgatgcct ggagagaata ttgtagagtt tgaacattat
    16801 tttaaaaatc aagtaaatga actgttagac tcaaatcaag aggttatcgt tttgactgac
    16861 ttgattggag gaagtcctaa taatgtggct ttgtcacggt ttttaaattt ggattcagtt
    16921 gatattgtaa cagggtttaa tatccctctc ctagtggaat taatatcaag ttatgattca
    16981 aaaatcaatt tagaagaaat tgttcaaaat gctcaaaata gtttgtttaa tgttaaacaa
    17041 caacttaacg tagaggagga agaggattta tgtctataga gtttgttcgt attgatgacc
    17101 gtctggtaca tggtcaagtt gtcactacgt ggctaaaaaa gtatgatatt gagcaagtta
    17161 tcattgttaa tgatcgcatc tcagaagata aaacacgaca atctatttta aagatttctg
    17221 caccggtagg tttaaaaatt gttttcttta gtgtaaaacg gtttgtggaa gttttaaact
    17281 ctgtgccaat aaaaaagaga acaatgctga tatatacaaa tccaaaagat gtgtatgatt
    17341 ctattgaagg aaatttaaaa ttggagtacc tcaatgtagg acagatgagt aaaacggagg
    17401 aaaatgaaaa ggtaacggga ggtgtagctc taggtgaaga agacaaatat tattttaaga
    17461 aaatagttga taagggaacg agagttgaaa ttcaaatggt tcctaatgat aaagttacaa
    17521 tgttggaaaa atttttataa aaataattta aggaggtaca gtatatgcta ttcacacaag
    17581 cattactggt gacattagtt gggattattg ccactattga ctataatgga ccgttattta
    17641 tgattcaccg tccgttagtt acaagtgcaa tggttggctt agtcttagga gatttcaccc
    17701 aaggtgttct tattggttca gctcttgaat taacttggct cggtgtaaca ggtattggag
    17761 gttacactcc accagatact atttcaggtg cgattattgg tactgcattt ggtattttat
    17821 ctggtcaagg agaaactgct ggtatcgcta tagcagttcc aattgcagtt gctacccaac
    17881 agttggatgt tcttgcaaaa actttagatg tttattttgt gaaaaaagct gataatgatg
    17941 ctaaaaacgg agattattca aagatcggtt tttatcatta ttcaagtttg gttttaatca
    18001 cgttatttaa aattgtacca attttcctag ctattatgct tggaggggaa tatgtggcag
    18061 acttgtttgc taaggttcca ccaatcgtta tgcagggact taactctgca ggtgctttac
    18121 taccttcaat tggttttggt atgcttttaa atatgatgct caagaaaaat atgtgggtat
    18181 tcttgttgat tggattcatt tgttctgtgt atggaggaat gtcaaccatt gggatctcac
    18241 tagttggtat tgcggtagca tacttctacg atatgattgg aagtaaacca caagaaacaa
    18301 cttcaagtag tgatgttgag gaggatcttg atctatgatg aataataaag taactaaagt
    18361 tgaacttaaa aaagttttca aacgaagttt tatgtatggt tcttcatgga actatgagag
    18421 aatgcagaac ctaggttttc tatatacaat tcttccagta ttgaaaaaac tatacccaga
    18481 caaagattca gcttctcctg caatgaaacg tcaccttgag tttttcaata ctcatcaaac
    18541 agcggcacca tttattcttg gagttacttc cgctatggaa gaacaagaag gaaatgaagg
    18601 tgcagcttca attactggta ttaaagttgg cttgatgggg ccactggctg gtctaggaga
    18661 tagtttgttc tggctgacac tagttcctat ctgttttagt attggtgcgt cttattctaa
    18721 agacggcggt gctttaggta tctttatcgc cttaatattg tttaatatta ttaatattcc
    18781 tgttaaatat ttcggtttga aatatgggta tactaagggt tctagtctta tccaagaaaa
    18841 taatacaaaa ggaacattga atcgcgttac gagtatggcg acagcattag ggctagtact
    18901 agtgggtggt ttgattccat caatggttgg tattaatttt ggattagaat ttaagcaggg
    18961 ggaacttgtt atttctgttc aagaaatgat tacaaaatta attccaggat ttatccctat
    19021 ggctttgact ttattaatgt gtaaattaat tagaaaagga aagaatccgg ttgtactaat
    19081 ctttagtgtt atggctattg gagttatcct agttgtttta ggaattttga agtagtagaa
    19141 agtgtggagg tggtatttgg gatatcacct ccattttgga agagaggtaa agagtgaaat
    19201 tatggtataa gaaagctgcc tcaaattgga atgaagcctt gccgattggg aacggtcatt
    19261 taggtggtat gatttatggt tcagctacaa aagaatgtat tcaactaaac gatgagacta
    19321 tttggtatag aggaaagtca gatagaaata atccagactc actattgcat cttaaaaaaa
    19381 ttcgggaata tcttttagat ggagaaattc agaaagccga agaattgata aagttaacaa
    19441 tgtttgctac cccaagagat caaagccact atgaattact tggggaactt tacattgagc
    19501 atatagatat tcagtcttgt gctctttcat tgtatgaaag agagctagat ttagatacag
    19561 ctatttctaa tgttgtgttt gagcctaata gttgtaattt acaaataaaa agagaatatt
    19621 ttacgagttt taataagaat attttatgtt gccgtatagt gtcatcagtt caaaacacat
    19681 taaatttaaa cattaatttg ggtagaaata aacggtttaa tgatgaagta tctaaactgg
    19741 attcaagtac aattttaatg tcggcctctg ctggaggtag aaaaggtgtt cagtttaaag
    19801 tagtatgtca ttctaaggtt acggatggtg aagtaagtgt attgggagag acaatagtta
    19861 ttcggaatgc tacagaggta tttctttatc tcaaatcaat gacggattat tggggaaata
    19921 tagatatttc ttctcttcag ggagaattta gtagtattga ttactttaca gaaaaagatg
    19981 aacatgtaaa aaaatatcag gagcaattta atagagttga ttttaaacta gactatagta
    20041 aagattgtct tagcattcca acgaatctac ttcttgaaaa cactaaaaag tatagtaact
    20101 acttgactaa cttgttattt cattatggaa gatatctgtt aatatcgtct agtcaaccga
    20161 atggtttacc tgccaatctt caaggaatat ggtgtgatga attaaatcca atttggggtt
    20221 ctaaatatac gattaatatt aatactcaaa tgaattattg gatggtaggt ccatgtgatt
    20281 taccagaagt agaatatcca ttatttgata tgctcgaaag aatgagagaa ccgggaagac
    20341 taaccgctaa gaaaatgtat ggagctagag gttttacagc acatcataat acggatggtt
    20401 ttggcgatac ggctccccaa tctcatgcca tgggggctgc aatttgggta ttaactattc
    20461 catggttatg tactcatatt tgggaacact atttatattt ccaagatgag cgtattctta
    20521 cggaacattt tgaaatgata aaagaagcat ttcttttctt tgaagattat ttatttgagg
    20581 tggatggcta cttgatgaca ggtccaagtg tctcaccgga aaataaatat cgcttaaaaa
    20641 atggtattga aggaaatgct tgtctatcat ctacaattga taatcaaatt ctaagatatt
    20701 tttgtgattc atgcattggc attgcaaaac aattaggaga caattcggat tttattagtc
    20761 gtgtgaagga gttaaaaaag aaactaccta aaacaaaaat aggtagtaat gggcaaatcc
    20821 aagaatggtt agaagattat gaagaagtag agcctgggca tagacacatt tcacctctat
    20881 ttgggcttta tccttataat gagattgata ttcataaaac tccggaatta gcagaagcag
    20941 ctaaaatcac tatcaatagg agattatcaa acgctaattt tttatcttca caggagaggg
    21001 agcaagcgat taataattgg ttagtaagtg gtttgcatgc tagtacacaa acaggttgga
    21061 gtgctgcatg gctgattcat ttttttgcga gactatatca aggtgaacct gcttataacc
    21121 agattaatgg tttgttaaat aatgcgactc ttggcaattt atttcttgac catccaccat
    21181 ttcaaattga tggtaattta ggtttggtga gtggaatttg tgaattatta gtacagagcc
    21241 atcataattg gttatcacta attccagctt taccttctgc ttggtcagaa ggagaagtga
    21301 aaggtttcag agtaagagga ggatataagg tatcgtttgc ttggaaaaat ggggatataa
    21361 cattcctaaa attggaagga ggaaacaaag atcaaaaagt aagagtaaga atatatggca
    21421 aaaatactga tgtacaaaat attgaattgg tatttaattc agaaaaaatt attgagttaa
    21481 atttttaggt ataagtcatg aataaagaaa aaataaaaag aaaattaatc acaatattgt
    21541 ttgtatgtat tgggatgtta tgttttggat tgttagcagg agttaaggct gataatcgtg
    21601 ttcaaatgag aacgacgatt aataatgaat cgccattgtt gctttctccg ttgtatggca
    21661 atgataatgg taacggatta tggtggggga acacattgaa gggagcatgg gaagctattc
    21721 ctgaagatgt aaagccatat gcagcgattg aacttcatcc tgcaaaagtc tgtaaaccaa
    21781 caagttgtat tccacgagat acgaaagaat tgagagaatg gtatgtcaag atgttggagg
    21841 aagctcaaag tctaaacatt ccagttttct tggttattat gtcggctgga gagcgtaata
    21901 cagttcctcc agagtggtta gatgaacaat tccaaaagta tagtgtgtta aaaggtgttt
    21961 taaatattga gaattattgg atttacaata accagttagc tccgcatagt gctaaatatt
    22021 tggaagtttg tgccaaatat ggagcgcatt ttatctggca tgatcatgaa aaatggttct
    22081 gggaaactat tatgaatgat ccgacattct ttgaagcgag tcaaaaatat cataaaaatt
    22141 tggtgttggc aactaaaaat acgccaataa gagatgatgc gggtacagat tctatcgtta
    22201 gtggattttg gttgagtggc ttatgtgata actggggctc atcaacagat acatggaaat
    22261 ggtgggaaaa acattataca aacacatttg aaactggaag agctagggat atgagatcct
    22321 atgcatcgga accagaatca atgattgcta tggaaatgat gaatgtatat actgggggag
    22381 gcacagttta taatttcgaa tgtgccgcgt atacatttat gacaaatgat gtaccaactc
    22441 cagcatttac taaaggtatt attcctttct ttagacatgc tatacaaaat ccagctccaa
    22501 gtaaggaaga agttgtaaat agaacaaaag ctgtattttg gaatggagaa ggtaggatta
    22561 gttcattaaa cggattttat caaggacttt attcgaatga tgaaacaatg cctttatata
    22621 ataatgggag atatcatatt cttcctgtaa tacatgagaa aattgataag gaaaagattt
    22681 catctatatt ccctaatgca aaaattttga ctaaaaatag tgaggaattg tctagtaaag
    22741 tcaactattt aaactcgctt tatccaaaac tttatgaagg agatgggtat gctcagcgtg
    22801 taggtaattc ctggtatatt tataatagta atgctaatat caataaaaat cagcaagtaa
    22861 tgttgcctat gtatactaat aatacaaagt cgttatcgtt agatttgacg ccacatactt
    22921 acgctgttgt taaagaaaat ccaaataatt tacatatttt attgaataat tacaggacag
    22981 ataagacagc tatgtgggca ttatcaggaa attttgatgc atcaaaaagt tggaagaaag
    23041 aagaattaga gttagcgaac tggataagca aaaattattc catcaatcct gtagataatg
    23101 actttaggac aacaacactt acattaaaag ggcatactgg tcataaacct cagataaata
    23161 taagtggcga taaaaatcat tatacttata cagaaaattg ggatgagaat acccatgttt
    23221 ataccattac ggttaatcat aatggaatgg tagagatgtc tataaatact gaggggacag
    23281 gtccagtctc tttcccaaca ccagataaat ttaatgatgg taatttgaat atagcatatg
    23341 caaaaccaac aacacaaagt tctgtagatt acaatggaga ccctaataga gctgtggatg
    23401 gtaacagaaa tggtaatttt aactctggtt cggtaacaca cactagggca gataatccct
    23461 cttggtggga agtcgatttg aaaaaaatgg ataaagttgg gcttgttaaa atttataatc
    23521 gcacagatgc tgagactcaa cgtctatcta attttgatgt gattctatat gacaataata
    23581 gaaacgaagt tgctaagaaa catgttaata atttgtcggg tgaatctgtt agtctagatt
    23641 tcaaagaaaa aggagcaagg tatattaaag ttaaattact aacgagtgga gtgcctttga
    23701 gtttagcaga agtagaggtt tttagagaat cagatggtaa gcaatctgaa gaggatatag
    23761 ataaaataac agaagataaa gtagtctcta caaataaggt agctactcaa agttcaacca
    23821 attatgaggg tgtagctgct ttagcagttg atggtaataa agatggagat tacggacatc
    23881 attcggtgac tcatactaag gcagattcta acgcttggtg gcaggtcgat ctgggagaag
    23941 agtttacggt ttctaaagtt gatatttata atagaacaga tgccgaacct cagcgtttat
    24001 ctaattttga tgttattttt ctatcttcat caggagaaga agtttttaga agacattttg
    24061 ataaagtagt tgatggtttg ttatctttaa aagtaccttc tgtaggggct aagctagtca
    24121 aaatagaatt aaaatcagca gctattccgt taagtttagc ggaagttgaa gtctatggtt
    24181 caaagagaac tccgaagaaa ctttctaata ttgcattaac caaagaaact cgacagagtt
    24241 caacggatta caatggtttt tctcgtctag cagttgatgg aaataaaaac ggagattatg
    24301 gtcatcattc agtgactcat accaaaggag attctccttc atggtgggag atagatttag
    24361 cacaaaccga agaattagaa aagttaatta tttataatag aacagatgct gaaattca
//
LOCUS       DAVKLW010000030        23874 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKLW010000030 DAVKLW010000000
VERSION     DAVKLW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.030"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(65..3241)
                     /gene="carB"
                     /locus_tag="VW324_001195"
     CDS             complement(65..3241)
                     /gene="carB"
                     /locus_tag="VW324_001195"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9726840.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3553..4632)
                     /gene="carA"
                     /locus_tag="VW324_001196"
     CDS             complement(3553..4632)
                     /gene="carA"
                     /locus_tag="VW324_001196"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9726841.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4682..5605)
                     /locus_tag="VW324_001197"
     CDS             complement(4682..5605)
                     /locus_tag="VW324_001197"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9726842.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5624..6145)
                     /gene="pyrR"
                     /locus_tag="VW324_001198"
     CDS             complement(5624..6145)
                     /gene="pyrR"
                     /locus_tag="VW324_001198"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9726843.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6356..6985)
                     /gene="nth"
                     /locus_tag="VW324_001199"
     CDS             complement(6356..6985)
                     /gene="nth"
                     /locus_tag="VW324_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9726844.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6985..7527)
                     /locus_tag="VW324_001200"
     CDS             complement(6985..7527)
                     /locus_tag="VW324_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9726845.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7696..7836
                     /locus_tag="VW324_001201"
     CDS             7696..7836
                     /locus_tag="VW324_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEU9726846.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQVTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            8251..9810
                     /locus_tag="VW324_001202"
     CDS             8251..9810
                     /locus_tag="VW324_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9726847.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9959..10858)
                     /gene="htpX"
                     /locus_tag="VW324_001203"
     CDS             complement(9959..10858)
                     /gene="htpX"
                     /locus_tag="VW324_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9726848.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(10860..11420)
                     /locus_tag="VW324_001204"
     CDS             complement(10860..11420)
                     /locus_tag="VW324_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9726849.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLMETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11514..12227
                     /gene="rsmG"
                     /locus_tag="VW324_001205"
     CDS             11514..12227
                     /gene="rsmG"
                     /locus_tag="VW324_001205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9726850.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12530..13813
                     /locus_tag="VW324_001206"
     CDS             12530..13813
                     /locus_tag="VW324_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9726851.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFIPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(14008..15579)
                     /gene="ffh"
                     /locus_tag="VW324_001207"
     CDS             complement(14008..15579)
                     /gene="ffh"
                     /locus_tag="VW324_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9726852.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15591..15923)
                     /locus_tag="VW324_001208"
     CDS             complement(15591..15923)
                     /locus_tag="VW324_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9726853.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(16014..16391)
                     /locus_tag="VW324_001209"
     CDS             complement(16014..16391)
                     /locus_tag="VW324_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9726854.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16463..17767
                     /locus_tag="VW324_001210"
     CDS             16463..17767
                     /locus_tag="VW324_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9726855.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17780..18586
                     /gene="yidA"
                     /locus_tag="VW324_001211"
     CDS             17780..18586
                     /gene="yidA"
                     /locus_tag="VW324_001211"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9726856.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(18641..18712)
                     /locus_tag="VW324_001212"
     tRNA            complement(18641..18712)
                     /locus_tag="VW324_001212"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18677..18679),aa:Arg,seq:cct)
     gene            complement(18754..19101)
                     /gene="rplS"
                     /locus_tag="VW324_001213"
     CDS             complement(18754..19101)
                     /gene="rplS"
                     /locus_tag="VW324_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9726857.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(19220..19549)
                     /gene="crcB"
                     /locus_tag="VW324_001214"
     CDS             complement(19220..19549)
                     /gene="crcB"
                     /locus_tag="VW324_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9726858.1"
                     /translation="MVIVYLAIACGFGALVRYLFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(19543..19917)
                     /gene="crcB"
                     /locus_tag="VW324_001215"
     CDS             complement(19543..19917)
                     /gene="crcB"
                     /locus_tag="VW324_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9726859.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(19914..20180)
                     /locus_tag="VW324_001216"
     CDS             complement(19914..20180)
                     /locus_tag="VW324_001216"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9726860.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(20296..20739)
                     /locus_tag="VW324_001217"
     CDS             complement(20296..20739)
                     /locus_tag="VW324_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9726861.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            20843..21778
                     /locus_tag="VW324_001218"
     CDS             20843..21778
                     /locus_tag="VW324_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9726862.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            21832..22116
                     /locus_tag="VW324_001219"
     CDS             21832..22116
                     /locus_tag="VW324_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9726863.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(22173..22751)
                     /locus_tag="VW324_001220"
     CDS             complement(22173..22751)
                     /locus_tag="VW324_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEU9726864.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKT
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(22726..23739)
                     /gene="efeB"
                     /locus_tag="VW324_001221"
                     /pseudo
     CDS             complement(22726..23739)
                     /gene="efeB"
                     /locus_tag="VW324_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
     gene            complement(23742..23874)
                     /locus_tag="VW324_001222"
                     /pseudo
     CDS             complement(23742..23874)
                     /locus_tag="VW324_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         7008 a         5375 c         4568 g         6923 t
ORIGIN      
        1 tagaaatatt ttagtattcc tgtgtactgt tctaaattca gtctgctata tcttattttt
       61 ctatttaaat cgcttctgta acaaagctac gactttcaag taccttaagc atggcattag
      121 ctgtatctag cgctgtgaag aggggcaccc cgtgttcaat ggctgaacga cgaatttgct
      181 caccatcttc gtcagcagtt cgttttgttc ctactgtgtt aatgatagct tgaattcttc
      241 ctttgcgtac aaaacttggg atatccttat cgtcatcacc aatcttacca acaggttggg
      301 cttgcaagcc atgactagca aagaaggctg ctgtcccttc tgtcgcaagg attccataac
      361 caatgttttg gaaacgacga gccaagttca aggcttcttc tttggcatca tcagcgatgg
      421 taaagacgac attaccaaaa gttggcaagt gtagataaga agcttcaaag gctttataga
      481 gagctttttc caaagtagca tcagaaccca taacttcacc tgttgacttc atttcaggac
      541 cgagcaagct gtctacctta gctagtttgg taaaggagaa gacaggtgcc ttgatatgaa
      601 cacgggtgct ttcagggtaa agtccatttt ggtagccaag ttctgataaa ctttgaccaa
      661 gaatgagctt ggtcgctact tgagccatag gaatattggt taccttagat aggaatggaa
      721 cagtacggct ggcacgtgga ttgacctcaa taacgtagac tttttcatcc ttgataacaa
      781 actggatgtt catcattcca aggcagtgaa gaccgattgc taagcgtttg gtgtagtctg
      841 cgatggtctc ctgaaccttt tgcgacaagg tttgtggtgg gtaaacagcc attgagtcac
      901 ctgagtggac accagcacgt tcgatatgct ccatgatacc aggaatgagc acatctttac
      961 catctgaaat ggcatcaact tcgcactctt gcccaacgat ataagagtcg acaagaactg
     1021 ggtgatctgg actagcctta acagcagttc gcatgtaaga acgaaggtct tcttcgtttt
     1081 caacgatttc catggcacgt ccaccaagta cataagatgg gcggacaaga actgggaagc
     1141 caatcttacg agctgcaaga gctgcttctt cttcattggt agccgtttgt cctggtggct
     1201 gtggaatatc caattctttg agagcttgct cgaagaggtc acggtcttcg gcacggtcta
     1261 ggtcagcaac ctgtgtacca aggatggtca cacctgcttt tgccaatggc tccgcaaggt
     1321 tgatggctgt ttgaccacca aactgaacga taactccctt tggttgttcc aagtcaatga
     1381 cgttcataac atcttcgaat gtcaatggct caaagtaaag cttatctgat acagagaagt
     1441 ctgttgaaac ggtctctggg tttgagttca tgatgatagc ttcataacca gctgcctgga
     1501 tagccttaac agagtgaacg gttgcgtagt caaactcaac cccttgaccg atacggattg
     1561 gacctgaacc taggacaagt acagattctt tatcagatct gatagattca ttttcccaac
     1621 cataggttga atagaaatat ggcgtttcag agtcgaactc tgccgcacaa gtatctacca
     1681 tcttataaac tggaacaatc ttgttttcca agcgaagttg gcgaacttga tcatcagtcg
     1741 ttccccagag ttcagcaatc ttacggtctg agaaaccatt aagtttggct gttttcaaaa
     1801 cttctagatc ttgtggatga gcacccaatt cttgctcaat ttcaaagata tgcaagagtt
     1861 tatcaagata gaagatatca atttttgtaa gctctgcaat ttcttcaggt gtgtagccac
     1921 gacgaatggc ttctgatacg tagaagagac ggtcatcttg ggctttgaca accttttcaa
     1981 tcaaggcatc atcagaaact gctgcaagtt caggtatttc attgtggtgc accccaattt
     2041 caagggagcg gcaggccttg agaagagatt cctcgatgtt acgaccgatt gccatgactt
     2101 ctccagtcgc cttcatttgt gtaccgagac ggcgttcacc cttttcaaac ttgtcaaatg
     2161 ggaaacgtgg aatcttagca actacgtagt caagggctgg ttcaaacatg gcataggttg
     2221 aacctgtaac tgggtttata acctcatcca aggtcaaacc tactgcaatc ttggcagcca
     2281 acttagcaat cggatatcct gtcgctttag aagcaagggc tgacgaacgt gatacacgag
     2341 ggtttacttc gataacataa tacttgaagc tgttaggatc aagagctagc tgaacattac
     2401 atccaccttc aatcttgagg gcgcgaataa tgctcaagct cgcatcacga agcatttggt
     2461 tttcatagtc tgacatggtt tgcgcagggg caaatacaat ggaatcccct gtgtgaatcc
     2521 caactgggtc aaagttttcc atgttacaaa caaccaaggc attgtcagct gagtcacgca
     2581 tcacttcgta ttcaatttcc ttaaaaccgg caatcgaacg ctcaatcaaa cattgggtaa
     2641 caggtgacaa tttcaaacca ttttcagtga tttcacgcaa ttctttttcg ttggcacaca
     2701 taccaccacc agtaccacca agggtaaagg ctggacgaac gatgactggg tagccaattg
     2761 tcgctgcaaa ggcaactgct tcttctactg tgttaacaat ttcagattct ggaatgggtt
     2821 gctcaagctc ttccatcaat tgtttaaaga ggtcacggtc ctccgcttgg tcaatggcag
     2881 ataatttggt acccagaagt tcaacgccaa gctcgtctag gataccattt ttagataatt
     2941 ccatggccat gttgagacct gtctgaccac cgagtgttgg tagcaaggca tctggacgtt
     3001 ccttacgaag aatacgtgtc acaaactcaa gtgtaatcgg ttcaatgtaa accttgtcag
     3061 caatttcctt gtccgtcatg atggttgcag gatttgagtt aaccaaaaca acctcataac
     3121 cttcctcttt caacgacaag caagcctggg tcccagcgta gtcaaactca gcagcctgac
     3181 caataataat cggaccagaa ccaatcacca taattttttg aatatcagta cgtttaggca
     3241 tagtttatga tacaaagccc gtaaagaaca cagtgaaaat aggaaactcg gcgcagacgc
     3301 cttcagcgtc aagacgatgt ttatcttttt cacacagttc ttagggcgtg ttcacttccg
     3361 cgaacaatgt atcttctcct ttctattcgt cgcctctcag ggcgacatta aataagatac
     3421 aaaggacgaa tagaaagcga ttgaatttta agaaatcaag gaaggattga caatccaagt
     3481 tggtttctct aaattctgag ctttccgtcc gtgttcagtt acataaattc tccgacgagc
     3541 ttttactcgt tcttagtttg attgtttaaa aacttccatc atctcgataa actcgtcaaa
     3601 taggtagcta gcgtcgtgtg gcccaggagc tgcatctggg tggtattgaa cagagaaagc
     3661 aggttggtat ctgtggcgca caccttccac tgacttgtca ttgatttctt cgtgggtaat
     3721 aatcaagtgc tctggcaaat cctcgcggct gactgcataa ccatggttct ggctggtgaa
     3781 gtctactcgt cctgttgcga tttcacgtac cgcatggttg aatccacgat ggccaaactt
     3841 catcttatag gtcttagccc cgtttgccat tgcaaagagt tggtgtccca tacaaatacc
     3901 aaagattgga atttttcctt gtacaccgcg aatcatgtcg agtgcttgtg gaacgtcttc
     3961 tgggttacct ggaccatttg acaacataac tccatcagga ttgagatgga gaatttcttc
     4021 agccgttgtc gaataaggaa caactgtcac gttacagttg cgtttagaaa gttcacgtag
     4081 gattgagtgc ttgagaccaa agtccactag caccacgctc aaaccaactc ctggagctgg
     4141 ataagacgtt ttagtagaaa cctgtttgat attgtctgtc ggtaaaactg ttgcttggag
     4201 ctggtccgtc acatggtcca tactgtcccc aacatgggtc aaggttgcac gcatagtacc
     4261 atgcttacgg ataatcttgg taagagcacg cgtatcaatt cctgaaatcc ctggaatttt
     4321 cttggctttc aaaaattcat ccaaggtcat ttggttgcgc cagttgctag ctctacgcgc
     4381 ttcttcaaaa acaacgactc ccttacaagt tggaataatg gattcataat catcacgatt
     4441 aataccataa tttcctacca aaggataagt aaaggtcaag atttgtccat tataagactg
     4501 gtctgtaatg gattcttggt agccggtcat ccctgtatta aagacgattt cgcctgttac
     4561 atcaatatct gctccgaagg ccttgccttc aaaaactgtg ccatcttcta atactagaat
     4621 tctttttgtc atattttcac ctctcgtgga cgctcactgg cgtcttttaa cgtcttgtgt
     4681 tttagttggc gtttctactc gctagtacgg attctaagat tgccattcga acaaagacac
     4741 cattggtcat ttgttggaca atccgtgatt ttggtgcttc aaccaagtgg tctgctattt
     4801 ctacatcacg attgattgga gctgggtgca tgaggattgc tgtttctttc aaacgatcgt
     4861 aacgttcttg agtcaagcca tgttgggcat ggtagtcttc ttttgaaaat acagctccac
     4921 tatcatggcg ttcgtgttgc acacggagaa acatcatgac atcaacctga tcaatgattt
     4981 catcaatggt tacaaactgt ccatagtctg caaactcttg acttctccat tcctcaggtc
     5041 cagcgaaaaa gagttcagct cccaagcgtt tcaaaatctg catattggat ttggcaacgc
     5101 gtgagtgatc caagtcacct gcaatagcaa ctttaagacc ctcaaagtgg ccaaattcct
     5161 cataaatggt catcaaatca agcaagctct ggctagggtg ttggcccgaa ccatctccac
     5221 cattgatgat ggaagtcgta atcgttggac tagcaatcaa ttctctatag tagtcgacct
     5281 ctggatggcg aatcacacag acatccactc ctaaagcaga cagagtcaaa atggtgtcat
     5341 aaagtgtctc acccttatta accgaactag tcttcacatc aaagtcaagt cgttccaatc
     5401 caagtttaat ctctgcgact tcaaaggact tatgtgtccg tgtagaatcc tcaaagaaga
     5461 gattggaaac aatcggatgg tcttcatagg gaagctgggc tccattttta aactcaattc
     5521 ctcgcttgat caatttcatt acttgatcga cagtgaggtc ttccatggac accacatggt
     5581 tcaatgcttg ttgattttct gacatggcta ctcctttaac tttctaagct tcttcagtaa
     5641 tcagaactct gtcttggtca tcaagttctg tcatctctac gatgatttct tcagaacgac
     5701 tggttgggat attttttcca acgtaatctg gacggattgg caattctcta tgtccacgat
     5761 cgactagaac tgctaaactc acacgcgcag gacgaccatg accgacaata ttatcaatag
     5821 cagcacggat ggtacgacct gtatagagca catcatccac caagataact tcgcggtctg
     5881 tcacatcgac agaaaccaaa gaagtatctt ctccactttt aacatcatca cggaaaggtt
     5941 tagtatccaa ttccacaaca ggaactgaaa gattttctaa ctgcttcaaa cgttcttgga
     6001 ttcggtgggc aataaagaca ccacgagttt taataccagc caagacgatc ttattcaaat
     6061 ctttgttgcg ttcgataatc tcataagtaa tacgcgtaat cgctcgtttg acggtcaatt
     6121 cgtctacaac ttcttttgtt ttcatgacaa acctccaaaa agaaaagtct ccttaaacaa
     6181 ggagacttga aatttatagc caagcgagcc ctactgcaca cagtgtagac ttcacccttc
     6241 tactttatcg cgctccttgc ctgcctcacg ggacaggttt aaaggaatat ttagttatca
     6301 tttactatag cacaaagcat gcttaaaatc aagcaaaaag tttcaatgta gcatcttaca
     6361 aattgctaaa atcatataat tgtgggtact ggtcacactc tggatttttt ggatggcaaa
     6421 tggctcttcc aaaataaatc atggcctgat gggcagctaa ccactgctca ggcggcaaga
     6481 tatccatgac ccgcttttcc acctcaagtg gcgtcgctga ttttttgaca atatcgtggt
     6541 gtttgcaaat acgctccaca tgagtatcca ctgcaaaggc tggaatccca aatcctacac
     6601 tcatgacaac attggctgtc ttgcgaccaa cacctgccaa actctccaat tcttcacgtg
     6661 tctgagggac ttgaccatca aaatcgtcta gtaactgttg ggcacatttt ttaaggaatt
     6721 tagctttatt ccgatacaat cccaagcgag aaatatgtga agcaatctca ctctctgtcg
     6781 ctacagacat agcttggggt gttggaaagg caacaaagag acctggtgtg gccttattta
     6841 ccgctgcatc tgtcgtctgg gctgataaca tgaccgcaac caggagttca aaatgattgg
     6901 taaaatcaag actaggcttg gcatctggga agagggcaat gatttcttct agcacctttc
     6961 gtgctcgttt ttttgacaag accattattc atctccgtca aatagtcctt gtaagccagc
     7021 aaaaggactg ttttcttctt tctttactgc ttgttgagct tggtattctt cctctgtcat
     7081 gatttgccag tcatttcctg agacaaatcc ttgaccagct tcttcttcag ccgtcaagac
     7141 cttgatagga atgtttagca ggatattgtc tgatacactc tcagcaaggt caagcgcccc
     7201 attttcgatg ggcaagacca agtcatcatc taaaacttct tgatctagct ggttagttgc
     7261 gccttccatg aaaacttccg tgactggata agattcaact aactcaactg gctccatact
     7321 gcgactcgaa gcaagaacaa tggtataaga tagttgataa tctaagaaat acatacggtc
     7381 ttcatattgt actttcccaa ctgcaaggat atcttttaca tctaaaattt cttgattacg
     7441 tgcacgcagg tcatcaacta aatctaacgt ttgttcaaag ttcaaacctt cagactgctt
     7501 acgaatttct tgaatattta atttcatact tcctccataa agatttactc tcttgattat
     7561 accatgaaaa ggctacaaat cagcacacca aactttgtaa ttaaaattca aattttaaca
     7621 tatttactat gatagtttta ttttttagtg ctatactata gggaaagagt acatcagatc
     7681 aaggaggatg ctcacatgga agacaagaaa ctcattcaac tcctatccaa gttaaataaa
     7741 agctaccaaa actgtaaaca ggttacggca gatgatattc gactacaaga gctgctaaac
     7801 actactatgc aagagctcaa aaaacagaaa agttgaacaa cagtatctta attaatcttg
     7861 agaaatttta ccaacccacc agtcctctga ttggactggg tagcctaaaa ctaaacaatc
     7921 aagcacgcac tgcttggcga aactatgata aaatccatta cgagtatgtc aaacacgtac
     7981 taagtctcta tggacctgtt tttggatttt agagcataga atttccagtt ttctgttgac
     8041 aaaatttcct taaaggtata atataaagat actaatactc ggaggtaagg gagacatgaa
     8101 caactaagtc tatcaaataa agaaccttta tttagtagat cttgtttttg tctctttttg
     8161 tgtgctcttt tatgctcttt ttctggcatg ttaatagagt ttttttgaca tagactttgg
     8221 gctctactag gtaaagtaga gctttttgtt atgcactatc gacattctag aaagggcaat
     8281 agtatgataa aaatcaatca cctgaccatc acacaaaaca aagatttacg agaccttgtc
     8341 tctgacctaa gcatgaccat acaagacggg gaaaaggtag ctattattgg cgaagaagga
     8401 aatggtaaat caaccttact taaaacttta atgggggaag atttgtctga tttcactatc
     8461 aagggagaca tccaatctga ctatcagtca ctggcctaca ttcctcaaaa acttcccgag
     8521 gagctgaaaa agaaaacttt acacgactac ttctttttag attctattga tttagactac
     8581 agtatcctct atcgtttggc ggaggaattg cattttgata gcaatcgttt cgcaagtgac
     8641 caagagattg gcaatctatc agggggagaa gctttgaaaa ttcagcttat ccatgagtta
     8701 gccaaaccct ttgagattct atttttagat gaaccttcaa atgacctaga ccttgagaca
     8761 gttgattggc taaaaggcca gattcaaaag accaggcaaa ccgttatttt catttcccat
     8821 gatgaagact ttctttctga aacggcagac actattgttc acttgcgact ggtcaaacac
     8881 cgtaaagaag cggaaacgct agtagagcat ttagactatg atagctatag tgagcagaga
     8941 aaggctaatt ttgccaaaca aagtcagcaa gctgctaaca accaaagagc ctacgataaa
     9001 accatggaaa aacatcggag agttaagcaa aatgtagaaa ctgcgcttcg agctaccaaa
     9061 gatagtactg ccggtcgcct attggctaaa aagatgaaaa ctgtcctctc acaagaaaaa
     9121 cgctacgaaa aggcagctca gtccatgact caaaaaccac ttgaagagga acaaatccaa
     9181 cttttctttt cagacatcca accattacca gcttctaaag tcttagtcca actggaaaaa
     9241 gaaaatttgt ccattgacga ccgagttttg gttcaaaaac tacaactaac tgtccgtggc
     9301 caagaaaaaa tcggtattat cgggccaaat ggtgttggga aatcaactct gttagccaag
     9361 ttacagagac ttctgaatga taaaagagag atttcacttg gttttatgcc acaagattac
     9421 cacaaaaaac tgcaattgga tttatcccca atagcctatc tcagtaaaac tggggaaaaa
     9481 gaggaactac agaaaatcca atctcaccta gctagtctca atttcagtta tccagaaatg
     9541 cagcatcaaa ttcgctcctt atctggcgga caacagggaa aactcctgct tttggattta
     9601 gtcctgcgca aaccaaactt tctcctgctg gatgaaccca cacgaaactt ttctcccact
     9661 tctcaacccc aaatcagaaa actctttgcc acttatcctg gcggtctcat cactgtttcg
     9721 catgaccgtc gtttcttaaa agaagtctgc tcgatcatct atcgcatgac agaacacggt
     9781 ttgaagctag ttaatttaga agatttataa atttgcaaca tagcagatag gtggggcaaa
     9841 aacacaattt taggagaaaa agaagtaaat cttcccatag taaaacgcat aatatcaagt
     9901 tttcacacct gatattatgc gtttttcaga ttttaaagac ttttttccag ccttcatttt
     9961 acatatgttt taaacgttca atccgttctg agataggtgg gtgggtataa aagagttttt
    10021 ggaacccccc acctttcttg ggatcattga tataaagggc actgctagca tcatcgacgt
    10081 ggcgactcat aggtttgcta ttgtccaact tatctagggc attaatcatt ccctggggat
    10141 tgcgagtcag ctcgacacta gatgcatctg ccagaaattc cctctgacga gaaatagcga
    10201 gctgaaccaa ggttgcagcg agaggtgcca gtacaatagc tagtagggaa accactagca
    10261 taatgatttc aagaccattt ccatctcggt catcatcact tcgtctgcga cctgctccac
    10321 cccaccacat catacgacct gccatactag aaagcatggt gatagcacta gcaagggcaa
    10381 ctgcaatagt cgaaatacgg atatcataat tacgaatatg actgacttca tgtcccataa
    10441 cagcttctag ttcttcacga ttcatgatag ctagtagacc tgaagtcgca gcaacagccg
    10501 cattttgagg attagaacct gtcgcaaagg catttaaggc tggatcatca atgatgaaaa
    10561 cacggggcat aggaatctga gcgaccagag ccatatcttc cactacatgg tagaggtctg
    10621 gtgccgtttg ctcatccacc tcacgcgctc cattcatgga catgacaatc tctgtcgatt
    10681 gaaaaatcat agacaaagcg tagataaagc cgataatcag tgcgataacc aaaccaccaa
    10741 gtccagatct tataaagaga taaccaaccg cataaccaac aagagctaag agtaggaaaa
    10801 ataccagcaa caaaatccag gtttttcgtt tattgcttgc aatttgatca aacaacatct
    10861 tagtcaccta aaccgctaaa atcaacttta ggaaccgact tttcctcttc aggtgtttga
    10921 aggaaatctg ccgctttaaa tccaaacatt ccagcgataa tattgctcgg gaaagtttct
    10981 aattttacat tgtagttgct gacaacactg ttatagagtt gacgagagta agaaatttta
    11041 ttttctgtgt ttgtcaactc ctcttgcaat ttaacaaagt tagcactagc tttcaaatct
    11101 ggatagcttt ctgcaactgc aaaaatacct gaaacttgac gagtgagggc atcactggct
    11161 ttcatagctt ctgctggtga agtcgctgcc gccacttggt tacgtagttc tgccaccttt
    11221 tcaagggtag aaccttcata tttggcataa ccttttacag tctccatcaa gtttggcaag
    11281 aggtcatttc gacgtttcaa ctgaacatca atctgactcc aagcctcctt ggtttgcata
    11341 cgattcttaa ccaaaccgtt atagctaaca atcacaaaaa taacaataag agcgataact
    11401 ccaagaataa tccaagtcat gatataagtc ctttctactt ttagattagt accagtatat
    11461 caaattttta atgattgtgg taaaataaga tgatactaaa gaaggaaata actatgaaac
    11521 cagaaacatt ttacaacttg cttgccgagc agaatcttcc actttcggac cagcaaaaag
    11581 aacaatttga acgttatttt gagctcttgg tcgagtggaa tgagaagatt aatttgacgg
    11641 cgattacgga caaggaagaa gtttatctca aacattttta cgattcgatt gcacccattc
    11701 ttcaaggttt gattcccaat gaaactatca aacttcttga tatcggggct ggggcaggat
    11761 ttcctagtct accaatgaaa attctctatc cggagttaga tgtgaccatt attgattcac
    11821 tcaataagcg catcaacttc ctacaactct tggctcaaga actggatttg aacggagttc
    11881 atttctacca cggacgtgcc gaagattttg cccaagacaa gaacttccgt gctcaatatg
    11941 attttgtaac agctcgtgcg gttgcccgta tgcaggtcct atctgaattg actattccct
    12001 accttaaggt tggtggaaaa ctattggcac tcaaggccag caatgcgcct gaggagttgt
    12061 tagaagctaa gaatgccctc aatctccttt ttagtaaggt cgaagacaat ctcagctacg
    12121 ccctaccaaa tagagatccg cgttacatca cagtggtaga aaagaaaaaa gaaacaccaa
    12181 ataaatatcc acgtaaggct ggtatgccaa ataaacgccc gctttaaatt tttaagtaaa
    12241 aaaatattta caaaatcaac ctcgctcttt tatttctagg ctcgggaaaa aatgatttac
    12301 aaaatcagcc tcgctctttt atttctaggc tcgggaaaaa atgatttaca aaatcatttt
    12361 tttctgctat actatcctaa gcaaaggttt ttaatgtcat cccgtgaggt gacgaagacg
    12421 caaaaatatt tgaaactctt taaagtctag attttagaga agtcttactc tgagggccta
    12481 ttgctgtaaa ataatgggct cttttctgat gcccaaaagt gaggtttata tgaaacaaga
    12541 atcaactgtt gatttgttat tagacgttga ccaacgtcct tcggctggaa aaggaattct
    12601 ccttagcttc caacacgttt tcgccatgtt tggtgcgacc atcttggtac cattgatttt
    12661 gggaatgcct gtatctgttg ccctttttgc ttcaggtgtt ggaacactca tctacatgat
    12721 tgctactggt tttaaagttc cagtttatct aggttcttca tttgccttta tcacagctat
    12781 gtcactggct atgaaagaaa tggggggtga tgtatctgct gcccaaacag gggttatctt
    12841 gactggtttg gtctatgtcc ttgttgctac cagcatccga tttgtaggaa caaaatggat
    12901 tgataaactc ttgccaccaa tcattatcgg tcctatgatc atcgttatcg gtcttggact
    12961 tgcaggttca gctgttacca atgcaggtct tgtagcagac ggaaattgga aaaatgctct
    13021 ggtagccgtt gttactttcc taattgctgc ctttatcaat acaaaaggaa aaggcttcct
    13081 acgaatcatt ccattcctct ttgccattat cggtggttac cttttcgcac taactcttgg
    13141 cttggttgac tttataccag ttcttaaagc caactggttc gaaattcctg gtttctactt
    13201 gccatttagc acaggtggtg cctttaaaga gtacaatctt tactttggtc cagaagccat
    13261 cgctatcttg ccaatcgcta tcgtaacaat ttctgaacat atcggagacc atactgtttt
    13321 gggtcaaatc tgtggtcgtc aattcttaaa agaaccaggt cttcaccgta ctcttcttgg
    13381 tgacggtatc gcaacttctg tttctgcctt ccttggtgga ccagccaata caacttacgg
    13441 agaaaataca ggggttatcg gtatgactcg tatcgcttct gtctcagtta tccgtaacgc
    13501 tgccttcatc gcgattgccc tcagcttcct tggtaaattc actgccttga tttcaactat
    13561 tccaaacgct gtacttggtg gtatgtcaat ccttctctat ggggttatcg ccagcaatgg
    13621 tttgaaagtc ttgattaaag aacgtgttga tttcgctcaa atgcgaaacc tcatcatcgc
    13681 aagtgctatg ttggttcttg gacttggagg agctatcctt aaacttggtc cagttacact
    13741 ttcaggtact gccctttcag ccatgacagg aatcatcttg aacttgatct tgccatacga
    13801 aaataaagac taagagtcta aatacaccta atccactcag acagctgagt ggatttttcg
    13861 tataccataa taaaagtgtc ttaacaaaat tattaaaatc aaaaaacgta taatatcaga
    13921 tattctaaaa ccttgatact gtacgtttta tcatagaaat ttttacttta ttttctcatc
    13981 aaatgagatt tgcatcaatc tcttgtctta cttgcgtttc ttcttcgctt tcttcatttt
    14041 gttagccata cgtttcatgg actgtttcat ggcaaattca ccaattttac ctttcaaacc
    14101 gccaccaaac atctggctca tatctggcat tcctgctcct ccgagagctg ataagtcagg
    14161 cataccgcct tgtcccatca ttccttcaag ggcagacata tccattcctc ccatatttgg
    14221 catattttta ggaaggttat ttggattaat ccccatttgc ttcatcattt tattcatatc
    14281 cccagacata acaccctgca tgagctgttt agcctggtta aagtccttga tgaatttatt
    14341 gacttcgacg aatgtatttc cagaaccagc agcaatacga cggcgacggc ttggatttaa
    14401 caaatctggg ttttcacgtt cttcaggtgt catcgaagac acaatcgcac gtttacgagc
    14461 aatctggcgt tcatccacct tcatgttttg aagggctgga ttgttggcca tacctggaat
    14521 catcttgagc aagtcttcca tcggccccat attttgcacc tgatctaatt gatcgatgaa
    14581 atcattaaaa tcaaaggtgt tttcgcgcat cttctcagcc atttcaaggg ctttttgttc
    14641 atcgtattcc tgagaagctt tctcaatcaa agtgagcata tcccccatac caaggatacg
    14701 gctagacatg cggtctgggt ggaaggtttc aatgtccgta atcttttcac ctgtaccagt
    14761 gaacttgatt ggttttccag taatgtgacg aacagacaga gcagcaccac cacgagtatc
    14821 gccatcaatc ttggtaagga tgaccccagt cacttccaac tgagcattaa actcacgcgc
    14881 aacattggct gcttcctgac caatcatagc atcaacgaca agcaagattt catttggttg
    14941 agccaatgct ttcacatcac gaagctcatt catgaggagc tcatcaatct gcaaacgacc
    15001 cgcagtatca atcaagacat agtcgttatg attagtttgg gcttgctcca aaccttgacg
    15061 tacaatctca acagctggta cttctgttcc aagtgcaaag acaggcacat caatctgttg
    15121 tcccaaggtc ttaagctggt caatggcagc tggacgataa atatccgccg caatcatcaa
    15181 aggacgagca ttttcttctt tcttgagttt gttggccaat ttaccagcaa aggttgtttt
    15241 accagcccct tgtaaaccaa ccatcatgat gatggttgga atcttaggtg acttgataat
    15301 ttctgccgta tcagaaccta aaacggctgt cagttcctca tcaacgattt taataatctg
    15361 ttgcgcagga ttaagtgtat caatgacctc atgcccgact gcacgctcac gaactttctt
    15421 gataaagtcc tttacaacag gcaaggcaac gtcggcctcg agcaaggcca agcgaatttc
    15481 tttggttgcc tcttggacat cagattcaga gatttttcct tttttacgta gatttttaaa
    15541 gacgttctgc aaacgttctg ttaaactttc aaatgccatt tttcttcctc ttattctcta
    15601 ttatcaatgc ttgttaaaat ttctatctgc tcctgcagaa agtcatcctt gggatagcgc
    15661 tccaaaatct gatcaaaaat ctgactgcgg acaatatagt ccgagtacat gtgcaatttc
    15721 atctcataat cttccagaat cttttctgtt cgcttgatat tgtcatagac agcctgacga
    15781 ctgacaccga actcctcggc aatttcagca aggctgtaat catcagcgta gtagagctcg
    15841 atataattca tttgcttatc tgtcaaaagc gccgcataaa attcaaagag cgcattcata
    15901 cgattggttt tttcgatttc cataactttt attataccaa aaattagcct aatctaccac
    15961 actaggaagc cgatccaaga agatagatag ctaaatttga aaaagacatg agcctagccc
    16021 caagtaattt ccaattgata gctggcaaag ggatgtccct cttgattttg tagttgataa
    16081 tctagttcaa tcttttgcct atcaacttga taatggctcg tttggatgat aaactcctgc
    16141 atgcccatag gtgtaggaat ataggctaaa ctatcgctat cctttagaaa gcgcataatg
    16201 gtcttgggat tagaaaatcg gctcatcaca agttcttgac catgaaattt aatcactact
    16261 ttttcctttt cctcattata gaaaagcagg tagctataat ctcctttttc atgcacttcc
    16321 acatcataaa gctggtcaat cacttccaac tgctcatcaa actgaattgt atttcgcatc
    16381 cgaatcttca catcaggtcc tctttcttgt ctcttgtcct actattttac caaaaagagc
    16441 aggattttgc tataatggtc atatgaacga aaaagtattc cgtgatccag ttcacaacta
    16501 catccatgtc aataatcaaa tcatctatga cttgattaac acaaaagaat ttcagcgttt
    16561 gcgccggatc aaacaactgg gaacttccag ttataccttc cacggtggag aacacagtcg
    16621 cttctctcac tgtctaggag tctatgagat tgcacgacgt atcacagaaa tttttgaaga
    16681 aaaatatcct gaggaatgga atcctgccga gtctctcttg accatgaccg ctgctctcct
    16741 acatgacctt gggcatggcg cctactccca tacttttgaa catctctttg atacagacca
    16801 tgaagccatt actcaggaga ttattcaaaa tcctgagaca gagattcacc aagtcttgct
    16861 acaagtagcg cccgattttc cagaaaaagt tgccagtgtc atagaccata cctatcctaa
    16921 taagcaggtc gtgcagctca tttctagtca gattgacgca gatcgcatgg actatctctt
    16981 gcgcgactcc tattttacag gagcatccta tggggaattt gacctgactc gcatcctccg
    17041 agtcattcgt cctgtcgaaa atggtatcgc ctttcagcgc aatggcatgc acgccatcga
    17101 agactacgtc ctcagtcgct accagatgta catgcaggtt tatttccacc cagcaacacg
    17161 cgccatggaa gttctcctac agaatcttct caagcgcgcc aaggaactct accctgagga
    17221 caagaatttc tttgcacgaa cttccccaca tctcctgcct ttcttcgaaa aaaatgtgac
    17281 cttgactgac tatctggctc tggatgatgg cgtgatgaat acctacttcc agctttggat
    17341 gactagtcct gacaaaattc ttgcagattt atctcaacgc tttgtcaacc gcaaggtctt
    17401 taaatccatt accttttcac aagaggacca agatcaactt actagcatga gaaaattggt
    17461 tgaggacatc ggctttgatc ccgactacta tactgccatt cataagaact ttgacctccc
    17521 ttatgatatc tatcgtcccg aatctcaaaa tccacggaca cagattgaga ttttacaaaa
    17581 aaatggagaa ctggccgaac tctctagcct gtctcctatc gtccaatccc ttgctggcag
    17641 tcgccacgga gataatcgct tttattttcc aaaagaaatg ttggaccaaa acagcatctt
    17701 tgcaagcatt acccagcaat ttttacactt gattgagaac gatcatttta ccccaaataa
    17761 aaactagaag aggaatttta tgagtattaa actaatcgct gtcgatattg acggaaccct
    17821 agtcaacagt caaaaggaga ttactcctga agtcttttct gccatccaag atgccaaaga
    17881 agctggtgtc aaagtcgtga ttgcaactgg ccgccccatc gcaggtgttg ccaaacttct
    17941 ggacgacttg cagttgagag accaaggtga ctatgtggtg accttcaatg gtgcccttgt
    18001 ccaagaaact gctactggcc atgagattat cagcgaatcc ttgacctatg aggattatct
    18061 ggatatggaa ttcctcagtc gcaagctcgg tgtccacatg catgccatta ccaaggacgg
    18121 tatctatact gcaaatcgca atatcggaac atacactgta cacgaatcaa ccctcgtcag
    18181 catgcctatc ttctaccgta cccctgaaga aatggctggc aaagaaattg ttaaatgtat
    18241 gtttatcgat gaaccagaaa ttctcgatgc tgcgattgaa aaaattccag cagaatttta
    18301 cgagcgctac tccatcaaca aatctgctcc tttctacctc gaactcctta caaagaatgt
    18361 agacaagggt tcagccatta ctcacttggc tgaaaaactc ggattgacca aagatgaaac
    18421 catggcaatc ggtgacgaag aaaatgaccg tgccatgctg gaagtcgttg gaaaccccgt
    18481 tgtcatggaa aatggaaatc cagaaatcaa aaaaatcgcc aaatacatca ctaaaacaaa
    18541 tgacgaatcc ggcgttgccc acgccatccg aacatgggta ctgtaaaagg aaagaataga
    18601 ctaaaaccgc tcagctgagc ggtttttgat gtctaattac tgccccctgc aggaatcgaa
    18661 cctgcaacta ctccttagga gggagttgtt atatccattg aactaaggga gctagataaa
    18721 aactctgctg aaaagcagag ttttttagtc gaattaacga cggatttctt tgatacgagc
    18781 tgctttacct tgaagagcac gcaagtagta caatttcgca cgacgtactt taccgtaacg
    18841 aacaacttcg attttttcaa cacgtggagt gtggattggg aagatacgct caacacctac
    18901 accgttagag attttacgaa ctgtgtagtt ttctgagatt ccagcacctt tacgtgcgat
    18961 aacaacacct tcaaaaatct ggatacgttc acggttacct tcgacaactt tcgcgtgtac
    19021 acgaacagtg tcaccaggac ggaatgatgg gatatctgta cgaagttgac cttcagtcaa
    19081 gctttggatt aatggattca ttttattctc ctatctttgt caatcttgag gaatcttcct
    19141 cagcggataa actgtatttt tgtgcgtcca ttacacacaa gatacagttt accaaatttc
    19201 taaaaaaaag taaagaaatt tatagcaaaa ttcctaaaaa aatcgcaacc aaaccaccta
    19261 tgtaagtcaa agtcaggtaa gaataaaaga ccttcttatc acttagcagt ctttgaagtt
    19321 cgtcattcaa ggtcgaaaaa gttgttaaac cgccacaaaa tcctgttgct agaatagcat
    19381 atacttcctt agactctaca tgattgtaaa atactccaat taaaaaacaa cctaaaagat
    19441 tagctatgag cgttccgagt ggcaatttag aagcttgatt atagcgagaa aataaatagc
    19501 gcactaaagc tccgaaacca caagcaattg caagatagac gattaccatt tcttcctccc
    19561 tagaaaataa gctaagagta ggcctccacc gatgctcaaa agcaaataca ggactaaact
    19621 aaaataacgc cctgtatcaa gcagcttcac agtatcaagc attaggctgg aaaaagttgt
    19681 taaacctccg caaaaacccg tccccagtgc taaaatcaag cccttactag tccccttata
    19741 gaccagatag cccttgacaa gaaaaatcaa gcagaaaatt cccagatagt tgacaaagag
    19801 agtgccccaa ggaaagtctg gactggctgg taaccaggtg gaaactagat atcggacaag
    19861 tccgcccaac atagcagcta gaaaaattcc tagcggataa aattgttctt ttttcatttg
    19921 atattttgat cctgataatc acgcgaacgt ttgagtatgt ccgaaaaagt cgctacaata
    19981 gtctcctgat agcgcttgtc ctctacacga ctcccaacct tttcaaaaat aacttcttct
    20041 ctcttagtat ctaaaatcgg tttacctgaa gctttcttat aagcaaccac cccctcaact
    20101 aaatgcattc gttcttctaa cagtttgaca atttggtcgt cgatttgatc gatttcttga
    20161 cgaataatat ctaaatccat acagtctcct cctttatttg aattattgta tcaaaaagcc
    20221 atcaattagg ctagtaaaat cccaactctc gataagaaaa atgcactgat tgattacatc
    20281 agcgcacttt tatgcttatc ctactttagc aaccaattct tctgcgaatt gttccaagcg
    20341 ttcaatatct tcttcctcgg cagaaagatc aactttaaca cactctgaac ctttttctgc
    20401 tcctgttgac acaaagacac ggtcaaagtc atcaacagcc ttacagaatt catcgtagaa
    20461 ggtatctcct gagccgacca ctccgtagat tttgccattc aagttgagat ctgctaggtc
    20521 ttcgtagaag tccatcatct catctggcaa ttcaccatca ccataagtat aggtcgcaac
    20581 gatagcgatg tctgcttcca agaagtctga agcgtcaaca gttgtacatt catcaacatc
    20641 aacatccaag cccaagtcac gtaatttgtc tgctacaata tctgcaattt cttcggtatt
    20701 accggtcata ctggcaaata caatttttgc taatgccata tcgtcctcct caatttatct
    20761 ttctccatta tatcacatct ttttcatttt aaaaataaaa attcttgtgc tacaatgaaa
    20821 tgagaaagaa ttgaggttat ttatggagat ttgccaacaa attttagaaa aaattaaaga
    20881 atacgacacc attatcattc atcgtcatat gaaaccagac cctgatgcct tgggaagtca
    20941 ggtgggattg aaagccttgc tagaacatca tttcccagaa aaaaccatca aagccgtcgg
    21001 ttttgatgaa ccaactctta cttggatggc tgagatggat cttgttgaag atagagccta
    21061 ccaaggcgca cttgtcatcg tctgtgatac agctaatact gctcgtatcg atgataagcg
    21121 ctatagtcaa ggtgattttc tcattaagat tgaccaccat ccaaatgatg atgtatacgg
    21181 tgacctgtct tgggtcgata ctagttcaag tagcgctagc gagatgatta ccctatttgc
    21241 ccaaacaacc caactagcct tggcagatcg cgatgctgag ttgctctttg caggaattgt
    21301 cggtgataca ggtcgcttcc tctacccttc taccactgca cggactcttc gcctggctgc
    21361 ttatttgaga gaacataact ttgactttgc ggctctcact cgcaaaatgg acactatgag
    21421 ctacaaaatt gctaaactac aaggctacat ctacgaccat ctggaagtgg atgaaaatgg
    21481 tgctgctcgc gttatcctga gtcagaaaat cttgaaacaa tacaatgtaa ccgatgctga
    21541 aactgcggcc attgtaggtg cacctggacg cattgacaga gtgagtctct ggggaatttt
    21601 tgtcgaacag gctgatggcc actaccgagt tcgcttacgc agtaaagtcc atcctatcaa
    21661 tgaaattgcc aaggagcatg atggtggagg ccaccctcta gcaagtggtg ctaattccta
    21721 tagcctagaa gaaaacgaaa tcatctacca aaagttaaaa aacttgctta aaaactgata
    21781 aaatacttgc caaacttttc agaatctgat agactagtat ggtaacaatc tatggctcgc
    21841 aaagagacca tggcagaaag gaaatattgc aaaatgaaaa aagatatcca tccagaatat
    21901 cgcccagttg tcttcatgga cacaactact ggttaccaat tccttagcgg ttcaacaaaa
    21961 cgctctaacg aaacagttga gttcgaaggc gaaacttacc cattgatccg tgtggaaatt
    22021 tcatcagact cacacccatt ctacaccgga cgtcaaaagt tcactcaagc agatggacgc
    22081 gtggatcgtt tcaacaaaaa atacggtctc aaataatgat aagagaacag ttttggctgt
    22141 tcttttttgt ttcttgaaat caactgctgt tttcatgttc cagactcatc tgtaggttcg
    22201 gtttccatgc tactaggcag gaaggaaata gctgtttcaa cacgtccata atgagctgta
    22261 ctattgtcac gaaccacact ttcattgatg gtccaagtgg aattcatttt cttaaaagct
    22321 tctcggactt tttccaaatc tttggaggca atggcctgtt ctaaggtttc aaaacgagga
    22381 cttagacggt ttaccaattt ttccttttcc gcatctaggt caacaggatt ttgttctttt
    22441 tcaaaagcta atagagctga ggaaatttcg gtgagtttgg cttcagtaat atctccagat
    22501 agagctaact tttccttaac aatcttacca acctcagaat ccttattttc tactctttcg
    22561 aagtctgatg ccatcttcgt aacgagtttc tgggtctcag tctgaccacc atttttaacc
    22621 gttttggatg catctgtgat tttcacaaag aaaaaactca agctttcttg ggcacctgct
    22681 ggggaaagaa ttaaaaatga caaagctaaa actagtaacc agcttttagc cttccaataa
    22741 tttctgacca atgtagcctc ctttctccac accaccaaag caagtaaaaa gtccactacc
    22801 tatgtgagtg atgtattcat tcatcttatc agtagcacct aggttagttt gaaccttaac
    22861 aaagttatca ggatccttct ggaaagaaat gaagagcaat cccgtatcaa actgaccagt
    22921 cttttcatca atgccatctg agtaagaata agaacgacgt aaaagtggct tatccacttc
    22981 tttggccaaa caaacatgcg aatcatcagg taagagactc aaatccactt catcaaactc
    23041 atttttctta ccaaaggggg caccgctttc cttgtaacga ccaaaggtat tttcttgttc
    23101 ttcgagacta gtgcggtccc aggtctctaa aaacatctga atccgacgaa ctgccatata
    23161 agaaccattt tccatccagt ccttactatc agcccagata acgcggtcaa agtcttgctc
    23221 cttggttgga tttgctgttc catccttaaa accaaagagg ttccacggag tctccattcg
    23281 atccccgata gctgcaaatc cagactgact ccaacgaagg ggcaccgcat ttctcccctt
    23341 acggatgaga ttgcgaatag cgtgaaaggc aatttgctca tcatctgcac aagcatgaat
    23401 cacaatatct ccaccagtat atttttcacg taattgctcc ttaggaaata gaggtaaatc
    23461 cctgaacagt cgagggcgct tgttttccaa gttcatcctt ttcaagaaac ttgcagatac
    23521 accaaaagtc agcgtcaaac gatgaggatt gagcccaact gtctcaccag tatcgctcgg
    23581 aggaaagaga gcattctgtc catctttttt aaccaattcc ccctcgacca acttggcact
    23641 ataatccgtc caatccttga atagctggat aattttatct ctatccgtcg tatgcaggtc
    23701 caagacaaca aaatagatat tcttttgcat gggcgtacta tttggtacat caagcgaacc
    23761 ttatgaaaag gtctactggt tttaaagcgc tttcttgtct tcttgaattg agattttatt
    23821 tttagacgcg acaatggttt gagcgtctgc tttcctagga tgttctggac gtgg
//
LOCUS       DAVKLW010000031        23397 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKLW010000031 DAVKLW010000000
VERSION     DAVKLW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.031"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            642..2795
                     /locus_tag="VW324_001223"
     CDS             642..2795
                     /locus_tag="VW324_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9726865.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2808..4157
                     /locus_tag="VW324_001224"
     CDS             2808..4157
                     /locus_tag="VW324_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9726866.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4327..5034
                     /locus_tag="VW324_001225"
     CDS             4327..5034
                     /locus_tag="VW324_001225"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9726867.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5091..8816
                     /locus_tag="VW324_001226"
     CDS             5091..8816
                     /locus_tag="VW324_001226"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9726868.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVVSDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8909..10351
                     /locus_tag="VW324_001227"
     CDS             8909..10351
                     /locus_tag="VW324_001227"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9726869.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10570..11592
                     /locus_tag="VW324_001228"
     CDS             10570..11592
                     /locus_tag="VW324_001228"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9726870.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIAGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11589..12134
                     /gene="purN"
                     /locus_tag="VW324_001229"
     CDS             11589..12134
                     /gene="purN"
                     /locus_tag="VW324_001229"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9726871.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12218..12727
                     /locus_tag="VW324_001230"
     CDS             12218..12727
                     /locus_tag="VW324_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9726872.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12752..14299
                     /gene="purH"
                     /locus_tag="VW324_001231"
     CDS             12752..14299
                     /gene="purH"
                     /locus_tag="VW324_001231"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9726873.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     KPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14421..15683
                     /gene="purD"
                     /locus_tag="VW324_001232"
     CDS             14421..15683
                     /gene="purD"
                     /locus_tag="VW324_001232"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9726874.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTAIYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQLILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLDYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16086..16574
                     /gene="purE"
                     /locus_tag="VW324_001233"
     CDS             16086..16574
                     /gene="purE"
                     /locus_tag="VW324_001233"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9726875.1"
                     /translation="MKPVISIIMGSKSDWATMQKTTEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16561..17652
                     /gene="purK"
                     /locus_tag="VW324_001234"
     CDS             16561..17652
                     /gene="purK"
                     /locus_tag="VW324_001234"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9726876.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17662..17889
                     /locus_tag="VW324_001235"
                     /pseudo
     CDS             17662..17889
                     /locus_tag="VW324_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
     gene            17952..19250
                     /gene="purB"
                     /locus_tag="VW324_001236"
     CDS             17952..19250
                     /gene="purB"
                     /locus_tag="VW324_001236"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9726877.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19305..23243)
                     /gene="strH"
                     /locus_tag="VW324_001237"
     CDS             complement(19305..23243)
                     /gene="strH"
                     /locus_tag="VW324_001237"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9726878.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMDFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQEEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6361 a         4665 c         5561 g         6810 t
ORIGIN      
        1 ctttctgatt acattatttt ttgataagaa taagaggaac tttggagata ttcttttatc
       61 taaaaatata accttaagtt ttagcatttt actttgttat ttatttaatc catcggatat
      121 aaagtttgcc tatttcagcg tattgacagt attactaatt gggataacaa attatgcgag
      181 atttaaaaat ttaagattga cagtagtgtt ttctgtacca tttttattta atgctataat
      241 gtctattata aatagttttt agaaatacta aaattttttt ctttacttaa taatgatatg
      301 aaatcatttc ggttttgtta acttgtaatt cttatatttt ctaggtagaa cgtttgttct
      361 gcctattttt ttatccaaaa agtgcagttg ggagggagat aggctcattt gggaaggaag
      421 tccagttttt gtttagtgat tggggtaaga tagttgttat cagatgagtt tatactcttc
      481 gaaaatcaaa ttcaaaccac gtcaacgtcg ccttgccgta tatatgtgac tgacttcgtc
      541 agtcctatct acaacctcaa aacagtgttt tgagcagcct gcggctagtt tcctagtttg
      601 ctctttgatt ttcattgagt attagggaaa aggagatgaa tatgaaattt gggaaacgtc
      661 actatcgtcc gcaagtggat cagatggact gcggtgtagc ttcattagcc atggtttttg
      721 gctactatgg tagttattat tttttggctc acttgcgaga attggctaag acgaccatgg
      781 atgggacgac ggctttgggc ttggtcaagg tggcagagga gattggtttt gagacgcgag
      841 ccattaaggc ggatatgacg ctttttgact tgccggattt gacttttcct tttgttgccc
      901 atgtgcttaa ggaagggaaa ttgctccact actatgtggt gactgggcag gataaggata
      961 gcattcatat tgccgatcca gatcccgggg tgaagttgac taaactgcca cgtgagcgtt
     1021 ttgaggaaga atggacagga gtgactcttt ttatggcacc tagtccagac tataagcctc
     1081 ataaggaaca aaaaaatggt ctgctctctt ttatccctat attagtgaag cagcgtggct
     1141 tgattgccaa tatcgttttg gcaacactct tggtaaccgt gattaacatt gtgggttctt
     1201 attatctgca gtctatcatt gatacctatg tgccagatca gatgcgttcg acactaggga
     1261 ttatttctat tgggctagtc atcgtctaca tcctccagca aatcttgtct tacgctcagg
     1321 agtatctctt gcttgttttg gggcaacgct tgtcgattga cgtgattttg tcctatatca
     1381 agcatgtttt tcacctccct atgtccttct ttgcgacacg caggacaggg gagatcgtgt
     1441 ctcgttttac agatgctaac agtatcatcg atgcgctggc ttcgaccatc ctttcgattt
     1501 tcctagatgt gtcaacggtt gtcattattt cccttgttct attttcacaa aataccaatc
     1561 tctttttcat gactttattg gcgcttccta tctacacagt gattatcttt gcctttatga
     1621 agccgtttga aaagatgaat cgggatacca tggaagccaa tgcggttctg tcttcttcta
     1681 tcattgagga catcaacggt attgagacta tcaagtcctt gaccagtgaa agtcagcgtt
     1741 accaaaaaat tgacaaggaa tttgtggatt atctgaagaa atcctttacc tatagtcgag
     1801 cagagagtca gcaaaaggct ctgaaaaagg ttgcccatct cttgcttaat gtcggcattc
     1861 tctggatggg ggctgttctg gtcatggatg gcaagatgag tttggggcag ttgattacct
     1921 ataatacctt gctggtttac tttaccaatc ctttggaaaa tatcatcaat ctgcaaacca
     1981 agcttcagac agcgcaggtt gccaataacc gtctaaatga agtgtatcta gtagcttctg
     2041 agtttgagga gaagaaaaca gttgaggatt tgagcttgat gaagggagat atgaccttca
     2101 agcaggttca ttacaagtat ggctatggtc gagacgtctt gtcggatatc aatttaaccg
     2161 ttccccaagg gtctaaggtg gcttttgtgg ggatttcagg gtcaggtaag acgactttgg
     2221 ccaagatgat ggttaatttt tacgacccaa gtcaagggga gattagtctg ggtggtgtca
     2281 atctcaatca gattgataaa aaagccctgc gccagtacat caactatctg cctcaacagc
     2341 cctatgtctt taacggaacg attttggaga atcttctttt gggagccaag gaggggacga
     2401 cacaggaaga tatcttacgg gcggtcgaat tggcagagat tcgagaggat atcgagcgca
     2461 tgccactgaa ttatcagaca gaattgactt cggatggggc agggatttca ggtggtcaac
     2521 gtcagagaat cgctttggcg cgtgctctct tgacagatgc gccggtcttg attttggatg
     2581 aggcgactag cagtttggat attttgacag agaagcggat tgtcgataat ctcattgctt
     2641 tggacaagac cttgattttc attgctcacc gcttgactat tgctgagcgg acagagaagg
     2701 tggttgtctt ggatcagggc aagattgtcg aagaaggaaa gcatgctgat ttgcttgcac
     2761 agggaggctt ttacgcccat ttggtcaata gctagaaaga ggagaggatg aaaccagaat
     2821 ttttagaaag tgcggagttt tataatcgtc gttaccataa tttttccagt agtgtgattg
     2881 tacccatggc ccttctgctc gtgtttttac ttggctttgc aactgttgca gagaaggaga
     2941 tgagtttgtc cactagagct actgtcgaac ccagtcgtat ccttgcaaat atccagtcaa
     3001 ctagcaacaa tcgtattctt gtcaatcatt tggaagaaaa taagctggtt aagaaggggg
     3061 atcttttggt tcaataccaa gaaggggcag agggtgtcca agcggagtcc tatgccagtc
     3121 agttggacat gctaaaggat caaaaaaagc aattggagta tctgcaaaag agcctgcaag
     3181 aaggggagaa ccactttcca gaggaggata agtttggcta ccaagccacc tttcgcgact
     3241 acatcagtca agcaggcagt cttagggcta gtacatcgca acaaaatgag accatcgcgt
     3301 cccagaatgc agcagctagc caaacccaag ccgaaatcgg caacctcatc agtcaaacag
     3361 aggctaaaat tcgcgattac cagacagcta agtcagctat tgaaacaggt acttccttgg
     3421 ccggtcagaa tctagcctac tctctttacc agtcctacaa gtctcagggc gaggaaaatc
     3481 cccaaactaa ggttcaggca gttgcacagg ttgaagcaca gatttctcag ttagaatcta
     3541 gtcttgctac ttaccgtgtc cagtatgcag gttcaggtac ccagcaagcc tatgcgtcag
     3601 ggttaagcag tcaattggaa tcccttaaat cccaacactt ggcaaaggtt ggtcaggaat
     3661 tgacccttct agcccagaaa atcttggagg cagagtcagg taagaaggta cagggaaatc
     3721 ttttagacaa ggggaaaatt acggcaagtg aggatggggt gcttcatctt aatcctgaga
     3781 ccagtgattc tagcatggtt gcagaaggtg ccctactagc ccaactttat ccatctttgg
     3841 aaagagaagg gaaagccaaa ctcacagcct atctaagttc aaaagatgta gcaagaatca
     3901 aggtcggtga ttctgttcgc tatactacga ctcatgatgc cgggaatcaa ctttttctag
     3961 attctactat tacaagtatt gatgcgacag ctactaagac tgagaaaggg aatttcttta
     4021 aaatcgaggc ggagactaat ctaacttcgg agcaggctga aaaacttagg tacggggtgg
     4081 aaggccgtct acagatgatt acgggcaaga aaagttatct acgttattat ttggatcaat
     4141 ttttgaacaa agagtaatgt tcgtgttttt agagttaaat aatttttaaa ctgtgagaaa
     4201 gattcttctt gcagtttttt ctttacaatt tttgaaaaac atctgctatt tattcggtta
     4261 aattcttgtg ttttttggtt ttttgtggta aaatgtgctc aagtaatacg aaaggcgaac
     4321 tttaaaatgt caaaacaatt gatctattcg ggaaaagcta aagatatcta tacaactgag
     4381 gatgaaaatc ttattatttc aacttacaag gaccaggcga ctgctttcaa tggtgtcaag
     4441 aaggagcaga ttgcaggtaa gggagtcttg aataatcaga tttcatcttt tatttttgag
     4501 aaattaaatg cggctggtgt ggcgactcac tttgtggaga aactttcaga cacggaacaa
     4561 ctcaataaaa aggttaagat tattcctttg gaagtcgtgc tccgcaacta tactgctggt
     4621 tccttttcaa aacgttttgg tgtggatgag ggaatcgcct tggagactcc gattgtcgaa
     4681 ttttactaca aaaatgatga tttggatgat ccatttatca atgatgagca tgtgaaattc
     4741 ctacagattg cggatgacca gcagattgcc tacttgaagg aagaaacgcg tcgtatcaat
     4801 gaactattga aagtctggtt tgctgagatt gggcttaaat tgattgactt taagctagag
     4861 ttcggttttg acaaggatgg caagattatc ttggcagacg aattttcacc agataactgc
     4921 cgcttgtggg atgctgatgg caaccacatg gataaggatg ttttccgtag aggattggga
     4981 gaactaaccg acgtttatga gattgtttgg gaaaagttgc agggtttaaa ataacaacct
     5041 caaggctgtt tgggaatatt gcaagagctg aaataaagga ataagaattg atggataaac
     5101 gtatttttgt tgaaaaaaag gctgattttc aggtcaagtc agagagtttg gttagagagc
     5161 tccagcacaa cttgggactg tcaagcttga aaagtattcg tattgtgcaa gtatatgatg
     5221 tatttgactt ggttgaggac ttgtttgcac ctgcagagaa gcacattttc tctgagcagg
     5281 taaccgacca tgttttagat gaagtatctg tgcaggcgga tcttgctaac tatgctttct
     5341 ttgccattga aagtctacca gggcagtttg accagcgtgc agcttcgtca caggaagcct
     5401 tgcttttgtt gggaagttcg agtgacgtga cagtcaacac agcccaactt tacttggtga
     5461 ataaagatat tgatgcgact gagttggaag ctgtcaaaaa ctacctgctc aatccagttg
     5521 attctcgttt caaggatatc acgataggga ttgccaagca ggagttttca gagtcagaca
     5581 agaccattcc aaaattgcct ttctttgaaa gctatacagc agaagacttt gctcgctaca
     5641 aggccgaaca agggatggcc atggaagtgg atgatttgct ctttatccaa gactacttta
     5701 agtcaatcgg gcgcgtgcca actgagactg aactcaaggt tttggacact tactggtctg
     5761 accactgccg tcatacgact tttgagacag agttgaaaca catcgacttt tcagcttcta
     5821 aatttcaaaa gcaattgcag tcaacctatg acaagtatat tgccatgcgc gaggaattag
     5881 gtcggtctga aaaaccacaa accttgatgg atatggcgac tattttcggt cgttatgagc
     5941 gtgctaatgg acgattggat gatatggaag tctctgacga aatcaatgcc tgctcagttg
     6001 aaattgaagt ggacgttgat ggtgtcaagg aaccttggct cctcatgttt aaaaacgaaa
     6061 cccacaacca tccaacagaa attgagccat ttggtggagc ggctacctgt atcggtggag
     6121 ctattcgtga tccgttgtca ggccgttcct atgtttacca agccatgcgt atttcaggtg
     6181 ctggtgatat tacagcaccg atttcggaaa ctcgtgctgg gaaattgcca caacaagtca
     6241 tttctaaaac agcaactcat ggttattctt catatggtaa ccagattggg cttgccacaa
     6301 cctacgttcg tgaatacttc cacccaggct ttgtagctaa acgtatggaa cttggtgccg
     6361 ttgttggtgc ggctcccaag ggcaatgttg tccgtgaaaa acctgaagca ggtgatgtga
     6421 tcatccttct cggaggtaaa acaggtcgtg atggtgtcgg tggtgcgacg ggctcttcta
     6481 aggttcaaac agttgagtct gtagagactg ctggtgctga ggttcaaaaa ggaaatgcca
     6541 tcgaagaacg caagattcag cgcctcttcc gtaatggcaa tgtcactcgt ctgatcaaga
     6601 agtccaatga ctttggggca ggcggtgtct gtgtggctat cggtgaattg gcagacggtc
     6661 ttgaaatcga cctcaacaag gtgcctctta aataccaggg cttgaatggt acagaaattg
     6721 ccatctctga atcacaagaa cggatggcgg tcgtggttcg tcctgaagat gtggatacct
     6781 tcgttgccga atgtaacaaa gaaaatattg atgctgttgt ggtggcgaca gtaactgaaa
     6841 aaccaaatct tgtcatgcac tggaatggtg agacaatcgt tgacttggag cgtcgtttcc
     6901 ttgacaccaa tggtgtgcgc gtggttgtcg atgccaaagt tgtggacaag gatgtcaaac
     6961 tcccagaaga gcgtcaaaca tctgctgaaa cactggaatc agataccctt acggttctat
     7021 ctgacctcaa ccatgcaagt caaaaaggat tacagactat ctttgactgc tctgttggac
     7081 gctcaacggt taatcaccca cttggtggtc gttaccaact cacaccaact gaggcatctg
     7141 tgcagaaatt gccagttcaa cacggtgtga ctcatactgc gtcggtcatt gctcaaggtt
     7201 tcaacccata tgtagctgaa tggtctccat accacggtgc tgcttatgcg gttatcgaag
     7261 caactgctcg tttggtggct gctggtgcca actggtctaa ggctcgtttc tcttaccaag
     7321 aatatttcga gcggatggac aagcaagcag agcgttttgg tcagccagta gccgcccttc
     7381 taggctctat tgaagcacaa attcagcttg gcttgccatc tatcggtggt aaggactcca
     7441 tgtctggtac ctttgaagaa ttgaccgttc cgccaacctt ggttgccttt ggggtgacga
     7501 cggcagatag ccgtaaggtg ctctctccag aatttaaagc tgttggggaa aatatctact
     7561 acatcccagg tcaagccctc tctgcagaga ttgattttga cttgattaag aaaaattttg
     7621 ctcagtttga agcaagccaa gctgaccata aagtgacatc tgcatcagct gtcaaatacg
     7681 gtggtgtagt tgaaagtttg gctcttgcta cctttggaaa ctatattggt gcagaggtga
     7741 ccttgcctga acttaaaaca gctttgacag ctcaattagg cggctttgtc ttcacatctc
     7801 ctgaagaaat tgctggagta gagaagattg gacaaacgaa agcagacttt acactgactg
     7861 tcaacggtgt gaagctagat ggacacaagc ttgacagtgc atttcaaggg acattggaag
     7921 aagtttaccc aacagaattt acccaagcga aagaactaga agaagtacca gctgtggtat
     7981 cagatgttgt gattaaagcc aaagaaaagg ttgaaaaacc tgtggtttac atcccagtct
     8041 ttccaggaac caactcagaa tatgattcag ctaaggcctt cgaaaaagaa ggtgcagagg
     8101 tcaatttggt gccattcgtg accttgaatg aagaagctat tgtcaagtca gttgaaacta
     8161 tggttgacaa tatcgacaag actaatattc tcttctttgc cggtggattc tcggctgcgg
     8221 atgaaccaga tggttcagct aagtttatcg tcaatatcct gcttaatgaa aaagtgcgtg
     8281 tggctattga tagctttatc gcccgtggtg gcttgattat cggtatttgt aatggattcc
     8341 aagccttagt caaatcgggt ctcctaccat acggaaactt tgaagatgct aacagtacta
     8401 gtccaaccct cttctacaat gatgccaacc aacacgtggc caagatggtg gaaactcgta
     8461 ttgccaatac caactcacca tggttggctg gtgtgcaagt gggcgatatc cacgctattc
     8521 ctgtttcgca cggtgaaggg aagtttgtcg tgacggctga ggaatttgca gagctccgtg
     8581 acaatggaca aattttcagc caatacgttg actttaacgg taaaccaagt atggattcta
     8641 agtacaatcc gaatggttct gtccatgcca tcgaaggaat taccagcaag aatggtcaaa
     8701 tcatcggtaa gatgggccac tcagaacgtt atgaggatgg tcttttccaa aatatcccag
     8761 gcaataaaga ccaacacctg ttcgcatcag cggttaaaca tttcactgga aaataagact
     8821 tacagatttt ctaatagata gtatcagtaa tgtaaaagtc atgtaaatct agctcttgat
     8881 gattacaaat gaaaattagg tataaaaaat gacatacgaa gtaaaatctc ttaatgaaga
     8941 atgtggtgtt ttcggtattt ggggacatcc agatgctgct aagttgacct attttggact
     9001 ccacagtctt caacaccgtg gtcaggaggg ggcaggaatc ctctccaatg atcaaggaca
     9061 actgaagcgc catcgtgaca tggggctttt atcagaagtt ttcagaaatc cagccaattt
     9121 ggataaattg acaggagctg gtgcgattgg gcatgtgcgt tatgcgactg ctggcgaagc
     9181 ttctgttgat aacatccaac ccttcctctt ccgttttcac gatatgcagt ttggtttggc
     9241 tcataatgga aatctgacca atgcagcctc tctcaagaaa gaactggaac aaagaggagc
     9301 aattttcagc gcgacttcgg actctgaaat cttggctcac ctcattcgtc gcagtcataa
     9361 tcctagcctg atgggcaaaa tcaaggaagc gctcagtctt gtcaaaggtg gttttgccta
     9421 tatcttgctg tttgaggaca agttgattgc ggctcttgac cctaatggct ttcgtccgct
     9481 ttctatcggg aaaatggcca acggagcggt ggttgtttcc tctgaaacct gtgcttttga
     9541 ggtcattggt gccgagtgga tttgtgattt gaagccaggt gagattgtga tcattgatga
     9601 cgagggcatt cagtatgaca gctatacaga tgatacccag ttggcgattt gttctatgga
     9661 gtatatctac tttgcccgcc ctgattctaa tatccacggt gtcaatgtcc atacggcacg
     9721 taagagaatg ggagcgcaat tggcgcgaga atttaagcat gaggcagata ttgtagttgg
     9781 tgtgcccaat tcttccctaa gcgcggctat gggatttgcg gaagaatcag gcttaccaaa
     9841 tgaaatgggt ctgatcaaaa accaatacac ccagcgaact tttatccaac cgactcaaga
     9901 attgcgggag caaggagtgc ggatgaaact gtctgctgtt tcgggtgttg tcaaaggcaa
     9961 acgtgtggtc atggtggatg attccattgt acgtggaaca acctctcgtc gtatcgttca
    10021 gctcttgaaa gaagcgggtg cgactgaggt tcacgttgcc attggaagtc ctgcactagc
    10081 gtatccatgt ttctacggga ttgatatcca gacccgtcag gagctgattg cagccaatca
    10141 tacggtcgaa gaaactcgcc aaatcattgg tgcggacagt ctgacttatc tttcaattga
    10201 tggcttgatt gagtcgattg gtatcgaaac agatgcgccg aacggtggtc tctgtgtcgc
    10261 ttactttgac ggtgactacc caacgcctct ctatgactac gaagaagact atcgtagaag
    10321 tttggaagaa aagaccagtt tttacaagta ggcgacagat tctccattaa agaaaaggaa
    10381 aaaacaaatg acaaataaaa atgcatatgc ctcacgtctc actactgact aaaggcttaa
    10441 gcatttagtc agtagacgct ttgtcctata ggatcaaagc tagagccctg actagtattt
    10501 ttagataaaa agatggttta tctaaaaata cgtcgcagtc tttctcaaaa aaagaaaagg
    10561 aaaaataaaa tggcaaataa aaatgcgtac gctcaatctg gtgtggatgt tgaagcgggt
    10621 tatgaagttg ttgaacggat taaaaagcac gtggctcgta cggagcgtgc aggtgtcatg
    10681 ggagctcttg gtggctttgg tggtatgttt gacctttcca agactggggt taaagaaccc
    10741 gtcttgattt cagggactga cggtgtcgga accaagctca tgttggctat caagtacgac
    10801 aagcacgata ccatcgggca ggactgtgtg gccatgtgtg tcaacgacat cattgctgca
    10861 ggtgcggaac ccctctattt tctcgactac gtagcgacag ggaagaatga accagctaag
    10921 ctagaacaag tggttgctgg tgtggcagaa ggttgtgtgc aggctggtgc tgccctcatc
    10981 gctggggaaa cggctgaaat gccgggcatg tacggcgaag acgactatga cttggctggt
    11041 tttgcggtcg gtgtggctga aaaatctcaa atcattgacg gttcaaaggt ggtagaggga
    11101 gatgttcttc tcggacttgc ttcaagtggg attcactcaa atggttactc tttggttcgt
    11161 cgtgtctttg cggattacac aggtgaggaa gtcctaccag aattggaagg caagaaactt
    11221 aaggaagttc tacttgagcc gactcgtatc tatgtcaagg ctgtcttgcc gctcatcaaa
    11281 gaagagttgg tcaacggcat tgcccacatc acaggtggtg gctttatcga aaatgtccct
    11341 cgtatgtttg cagatgacct agctgctgaa attgatgaaa gcaaagttcc agtgcttcca
    11401 attttcaaag cccttgaaaa atatggtcag attaaacacg aagaaatgtt tgaaatcttc
    11461 aatatgggtg tgggacttat gttggcggtc agccctgaaa atgtagagcg tgtaaaagaa
    11521 ttgttggatg aagcagtcta tgaaattggt cgcatcgtca agaaagaaaa cgaaagtgtc
    11581 attatcaaat gaaaaaaata gcggtttttg cctctggtaa tggctcaaat tttcaggtga
    11641 ttgccgaaga atttccagtg gagtttgtct tttcagacca tcgtgatgcc tatgtgcttg
    11701 agcgtgcaaa gcagctcggc gttctgtcct atgcttttga actcaaggag tttgagagca
    11761 agacagacta cgaagcagcc cttgtcgaac tcttggaaga acaccagatt gacttggttt
    11821 gcctagcagg ctacatgaaa atcgttggac caaccttatt gtcggcttat gaaggtcgga
    11881 ttgtcaacat tcatccagcc tacttgccag aatttccagg agctcatggg attgaggatg
    11941 cttggaatgc tggcgtgggt cagtctggtg tgaccattca ctgggtggat tcgggtgtgg
    12001 atacaggcca ggtcatcaaa caggttcgtg tgccacgact agctgatgat accattgaca
    12061 gatttgaagc tcgcatccat gaagcagagt acaggttgta tccggaagta gtgaaggctc
    12121 tatttacaga ttgacttttt gatgattcat atgatatctt tgattttaaa ttggagtcag
    12181 tgtttgttga agacggcttc aaacggaggt atttgtaatg ttagaatcta aaaaaacaac
    12241 tcgatatgta ttttatgtct atctgatgtt attaacttgg ggaatcttat ttaagtttga
    12301 aacaaatcct gaatttatag catttttctt agctccaagg tatatcaatt ggattccatt
    12361 ttcagaacca ctaatagtcg atggaaaaat tgtttttgct gaaatgttat ttaatctgat
    12421 ttcctttatt ccattaggtg tttgtttccc tttgataaaa actaatttat ctagtttaag
    12481 aatagtcggg acaggtttct tgattagttt attgtttgag tgcttacagt atattttagc
    12541 aataggtata acagatataa cggatttgac tttaaatacg ctaggtgtct gtgtaggctt
    12601 actgatttat caaattttta taagagtgtt caaatcacag actagaaaat ggatcaatat
    12661 cttaggtatg cttagccttg gttttgctta tcttgtttta ctgttactgc atttaattgg
    12721 tgtttaacta atgattaaaa aggagaatat aatgactaaa cgcgtcttaa tcagcgtctc
    12781 agacaaagcg ggcattgttg aatttgccca agaactcaaa aaacttggtt gggagattat
    12841 ctcaacaggt ggaactaagg ttgcccttga taatgctggg gtggatacca ttgctatcga
    12901 tgatgttact ggtttcccag aaatgatgga cggtcgtgtg aagaccctcc acccaaatat
    12961 ccacggaggg cttctcgctc gtcgtgactt ggatagccac ttggaagcgg ctaaggacaa
    13021 caagattgag ctcattgacc ttgtggtggt caacctttac ccatttaagg aaactatcct
    13081 taaaccagat gtgacttatg ctgatgcagt tgaaaatatc gatattggtg ggccgtctat
    13141 gcttcgttca gcagcgaaaa atcatgccag tgttacagtt gtggtagatc ctgctgacta
    13201 cgctgtggtt ttggacgaat tggcagcaaa cggcgaaacc tcttatgaaa ctcgccaacg
    13261 tttagcagcc aaagtatttc gtcacacagc ggcttatgac gccttgattg cagaatactt
    13321 cacagctcaa gtgggtgaaa gcaagcctga aaaactcact ttgacttatg acctcaagca
    13381 accaatgcgt tacggtgaga atcctcaaca agacgcggac ttttaccaga aagctttgcc
    13441 tacagactac tccattgctt cagccaaaca gctcaacggg aaagaattgt catttaataa
    13501 tatccgtgat gcagatgctg ctatccgtat catccgtgac ttcaaagata gtccaaccgt
    13561 tgtggctctc aaacacatga aaccatgtgg aattggtcaa gctgatgaca tcgagactgc
    13621 ttgggactac gcttatgagt ctgacccagt gtctatcttt ggtgggattg tcgtcctcaa
    13681 ccgtgaggtg gatgctgcga cagctgagaa gatgcacggc gttttcctcg aaatcatcat
    13741 tgcaccaagc tatacggatg aagcgctagc cattttgatc aataaaaaga aaaacttgcg
    13801 tatccttgcc ttgccattta atgctcaaga ggctagcgaa gtggaagcag aatacacagg
    13861 tgtagtcggt ggacttctcg tgcaaaatca agacgtggtc aaggaaagcc cagctgactg
    13921 gcaagtggtg actaaacgtc agccaactga gacagaagcg actgctcttg agttcgcttg
    13981 gaaggctatc aagtacgtca aatcaaatgg tattatcgtg accaacgacc acatgacact
    14041 tggtgttggt ccaggtcaaa ccaaccgtgt ggcttctgtt cgccttgcca ttgaccaagc
    14101 caaagatcgt ctggacgggg cggtccttgc ttcagatgcc ttcttcccat ttgcggataa
    14161 cgtggaagaa atcgccaaag caggaattaa ggccatcatc cagcccggtg gctctgtccg
    14221 tgaccaagaa tccatcgaag cagcggataa atacggcttg actatggtct ttacaggtgt
    14281 gagacatttt agacattaag aagataaaag ggaagaaaac agtttctttc cttttttggc
    14341 ttaaaatgct aactgaaaca agattaaaac gaactttttt gatataatgt tggtaaataa
    14401 ttcgcaaaag aggttgagga atgaaactgc ttgttgtcgg ttctggtggt cgtgagcatg
    14461 cgattgctaa aaagttactt gaatcaaaag acgtggaaaa agtctttgta gctcctggga
    14521 atgatgggat gactctggat ggtttggaat tggtaaatat ctctatttcc gaacattcta
    14581 aattgattga cttcgcaaag accaatgatg ttgcttggac ctttatcggt ccagatgatg
    14641 cccttgctgc tggcatcgtg gatgatttta accaagctgg acttaaggcc tttggtccga
    14701 ctagggctgc agcggagctg gagtggtcca aggatttcgc caaggaaatc atggtcaaat
    14761 acggcgttcc gacagcaata tatggcacat tttcagattt cgaggaagcc aaggcctata
    14821 tcgaaaagca tggtgcgcct atcgtagtca aggcggatgg cttggcactt gggaagggtg
    14881 tcgtcgttgc tgagacggtt gagcaagcgg tcgaagccgc tcatgagatg cttttggaca
    14941 ataaatttgg tgactcaggt gcgcgcgtgg ttattgagga attccttgaa ggcgaggaat
    15001 tttcactctt tgcctttgtc aatggtgata agttctacat catgccaacg gctcaggacc
    15061 acaaacgtgc ctatgatggc gacaaagggc ctaacacggg tggtatgggt gcctatgcgc
    15121 cagttccaca cttaccacag agtgtagttg atacagcggt tgacaccatt gtcaagccag
    15181 ttctagaagg ggtgattaaa gaaggtcgtc cttatctggg agttctttac gcagggctta
    15241 tcctgacagc tgatggaccg aaggtcatcg agttcaacgc tcggttcgga gatccagaaa
    15301 ctcagcttat cttgcctcgc ttaacctctg actttgctca aaatatcaca gatatcctgg
    15361 atagcaagga gccaaatatc acgtggacgg acaagggtgt gactctgggt gtggttgtcg
    15421 catccaaggg ctacccgcta gattattcaa agggtgttga gttgccagtc aaaaccgatg
    15481 gcgacatcat tacctactat gcaggggcta agtttgcgga aaatagcaga gcactgctct
    15541 caaacggcgg acgagtttat atgctcgtta ccacagcaga taccgtcaaa gaagcccaag
    15601 ccagcatcta ccaagaacta tcccaacaaa aaatagaagg actcttctac cgaacagata
    15661 tcggaagcaa ggcaattaag taaagatata agaataacgc gccgtagtcg ccaaacacga
    15721 taatggtcgt cgtggtgaaa agaccagaac agtgaatgtt ctggtcaggg ggaaacttgg
    15781 agaccgtagg ctcaaagttt aggaatgaaa ccgaaggttt gcttccgcct ccatcaccta
    15841 agaccattat caaaaagaaa aataaaaatt cacaaaatac gttaatggtc gtatggtttg
    15901 cgagcgttag cgagctaata tagaataatc accgccgttg tgaaagaacg attggatgac
    15961 aatccaatcg ttcagggaaa ttggaagacc ttgggtttcc aatttaggca tgagacacct
    16021 ttggtggttg ctgccgtccc tcacaagcta aggtgattgt tgaaaaagag gaaaaaggag
    16081 aagaaatgaa accagtaatt tccatcatca tgggctcaaa atccgactgg gcaaccatgc
    16141 aaaaaacaac agaagtccta gaccgcttcg gtgtagccta cgaaaagaaa gttgtttccg
    16201 cacaccgtac accagacctc atgttcaaac atgcagaaga agcccgtagt cgtggcatca
    16261 agatcatcat cgcaggtgct ggtggcgcag cgcatttgcc aggcatggta gctgccaaaa
    16321 caacccttcc agtcattggt gtgccagtca agtctcgtgc tcttagtgga gtggattcac
    16381 tctattctat cgttcagatg ccgggtgggg tgcctgttgc gaccatggct atcggtgaag
    16441 ctggagcgac taacgcagct ctctttgccc tccgtctcct ctctgtagaa gataagtcca
    16501 ttgcggatgc acttgccaac tttgctgaag aacaaggaaa aatcgcagag gagtcgtcaa
    16561 atgagctcat ctaaaacaat cggaattatc ggtggcggtc aactgggtca gatgatggcc
    16621 atttctgcta tctacatggg ccacaaggtt atcgcgctgg atcctgcggc ggattgcccg
    16681 gcctctcgcg tggcggaaat cattgtggca ccttataacg atgtggacgc cctccgtcag
    16741 ttggcagacc gttgcgatgt cctcacttac gagtttgaaa atgtcgacgc tgacggtttg
    16801 gatgccgtta tcaaggatgg acaactccct caaggaacag atctgctccg catttcgcaa
    16861 aatcgtattt ttgaaaagga ctttttgtca aacaaggctc aagtcactgt ggcaccctac
    16921 aaggtcgtga cttctagcct agacttggca gatatcgact tgtcgaaaaa ctatgtcctc
    16981 aagactgcga ctggtggcta cgatggtcat ggacaaaagg ttattcgttc agaagcagac
    17041 ttggaagcag cctatgcgct agcagactca gcagactgcg tcttggaaga atttgtcaac
    17101 tttgaccttg agatttctgt catcgtgtca ggaaatggca aggaggtgac gtttttccca
    17161 gttcaggaaa atatccaccg caacaatatc ctgtctaaga ccatcgtacc agcccgcatt
    17221 tctgaaagtc tagtagacaa ggctaaagct atggcagtgc gaatcgcaga acaactcaac
    17281 ttgtctggaa ctctctgtgt ggaaatgttt gcgactgatg atgacgttat tgtcaatgaa
    17341 atcgccccac gaccacataa ctctgggcac tattctattg aagcctgtga tttctctcag
    17401 tttgacaccc atattctcgg tgttctcgga gcaccattac cagtcatcaa actccatgcg
    17461 ccagccgtta tgcttaatgt cctcggtcag catgtcgagg ctgctgagca atatgtgaca
    17521 gaaaattcaa gcgcccacct ccacatgtat ggtaaaatag aagcgaagca caaccgcaag
    17581 atgggacatg tgaccttgtt tagtgatgta ccggatagtg tggatgagct tggggaaggg
    17641 attgattttt aggacaagtc tatgatacaa attatcgtta atacatttat tgaaaaggat
    17701 aagactggag cagttgttga agtgttgtat gccagtgctg accaagataa ggtacaagct
    17761 aaatatgaag aactagctgc ataatacccc gaaaattatt tagctatcta tgatgtaccg
    17821 ctggatacgg atttgaatac actagatcat tacccgtctg tgtttattga aaaagaggag
    17881 tttgagtaga aatcttggtt tacctagata gcttattccc aacagcttaa gaagaaagga
    17941 aaaattaaca catgatcaac cgttactctc gccctgagat ggcgaatatt tggagtgaag
    18001 aaaataaata ccgtgcttgg cttgaggtgg aaatcctctc tgacgaggca tgggctgagt
    18061 tgggggaaat ccctaaggaa gatgtggctt tgattcgcaa gaaggcggac tttgacatcg
    18121 accgtatttt ggaaattgag caggagacgc gccacgatgt ggtggctttc acgcgtgcgg
    18181 tttctgagac tcttggtgaa gagcgcaagt gggttcacta tgggttaact tctaccgacg
    18241 tggtggatac tgcttatggt tacctctaca agcaggccaa cgacatcatc cgtcgtgacc
    18301 ttgaaaactt cactaatatc atcgctgaca aggccaagga gcacaagttc accatcatga
    18361 tggggcgtac tcatggtgtg cacgctgagc cgacaacctt tggtcttaaa ttggcaactt
    18421 ggtacagcga aatgaaacgc aatatcgagc gtttcgagca tgcagctgct ggtgtagaag
    18481 ctggtaagat ttctggtgcg gttgggaact ttgccaatat cccaccattt gtagaggagt
    18541 atgtctgcga taaacttggc atccgtgccc aagaaatctc tacacaagtc cttcctcgtg
    18601 accttcacgc tgagtacttt gcggttcttg ccagcattgc gacttcaatc gaacgtatgg
    18661 cgactgagat tcgtggtcta caaaaatctg agcaacgcga agtagaagag ttctttgcta
    18721 aagggcaaaa agggtcttca gcaatgcctc acaaacgcaa cccaatcggt tctgaaaata
    18781 tgactggtct ggcgcgtgtc attcgtggtc acatgattac ggcttatgaa aacgtcgctc
    18841 tctggcacga acgcgatatt tctcactcat cagctgagcg tatcatcaca ccagatacga
    18901 ccattttgat tgactacatg ctcaaccgtt ttggaaatat cgtcaagaac ttgacagtct
    18961 tcccagaaaa tatgatccga aacatgaact cgacttttgg tcttatcttt agccaacggg
    19021 ctatgttgac attgattgaa aaaggcatga cccgtgagca agcctatgac ttggtgcaac
    19081 caaaaacagc ctactcttgg gacaaccaag tagactttaa accacttctt gaggcagatt
    19141 cagaagtaac atcacgtctc actcaagaag aaatcgatga aatcttcaac ccagtttatt
    19201 acaccaaacg agtggatgat atctttgaac gtcttggact aggtgattaa ttaaaaaata
    19261 aacagcgagc ttcaatctcg ctgtttattt tttatcgaaa agacttagtc ttcttttctt
    19321 ttagtgagtc cataggctgc tagtgtggac atgagtcctg cgactactag tcctgcagaa
    19381 tcgtgagttc ctgtttcagg aagttttttc tctgttacca caggagctgg atcttgagga
    19441 agagctttgc tttcctcagc aggagcagtt gatggagctg gttggcttgg gatttctagt
    19501 tttggttttt cttcagcaat agcggcttgt ccgttttcat cgcctacatg tgttaccata
    19561 gttccgactt cgactatttg agtaacggct tcctgtgcta cgacactatt tacaagtgtt
    19621 ttcacttcct taccatcggc agaagtgctc acagagtaga agttgctacg atgtccattg
    19681 acgcccttag taatgacttg tgtttttcct ttgagtaaga gtggattttc acgagtcact
    19741 gtggtaaatg gaatttcttc tttttggata tccagtctag gttttacctc agtagttggt
    19801 gcaagaccac tttcatcacc cttgtgagtt acaggagcgc caacttcaac cacttggttt
    19861 ataacttctt tggttacctg gctatcaagg actgtttctg ttgtttttcc attttcagtg
    19921 agtacagaga tgtaatgagt tcgttcacct ttgactcctg ctgtgataat attttcttga
    19981 ccagctggga ggttaggatt ttctttcttg ataacttcaa atggaatttc ttcagttctt
    20041 gtgatgagtt ctggtctggt ttcaacattg gcagccactt cattttcatc taggcttcct
    20101 gaatgagttg cagctggttt gaggccttga agagcggctt ttaggttggc tacaagtgtg
    20161 tcaagctcag cttgtttatt acggttgagg ttgtaattta gagctgtttt agctgcgtca
    20221 agggcctcaa gactttcttt actatatcct tctaagtttg taggaatttt agctaattct
    20281 tcgcggagag cattataatt agcacggaag tagtctttgt tgtggtctgc aaaggcagtc
    20341 atgagttcaa agatttcctc ttccttgtat tcagcgcttg gtctatctgc ccagattgaa
    20401 agcatacttc cgactgttgg aagatctact tcaggatatt tggtagaagc tagttgattg
    20461 aatggaactt taccagaatt ttcaactgct tttgaaagag ggtaagcttc ctcttgtttg
    20521 tgattgccta taacataata ccagtcaccg ttggtattca agaatttata gcctttgctt
    20581 gctaggtatt gaggtgatgc gaggttatat ccccaccagc ctttagacca gtaagaaatc
    20641 aagacatctt tgtcaaactg aacatcgtcc ttgtcttcat agtagaagcc atcgttgaag
    20701 gccattggtt gaagccctct ttctttggcc atagctgcga gggtgttggc atattcggca
    20761 aatttgccat agagttgata ccacttgagg tagtaccagc cttgggcact agtcgcatcg
    20821 ttggcgtatt cgtcagtacc aaagttgaaa atctttgttt tacctgcaaa gaagtccatg
    20881 tatttaccga tgagggcttt tacaaagtcc atcgcttctt cgtttttcaa gtccatagtt
    20941 gtttttgaaa ctttatcaaa gtgggcttga ggatttttaa tacctaattt ttccatggca
    21001 accagcatag catccatgtg acctggactg ttaatagctg ggatgagacc gatgtcctta
    21061 gatttagcgt attcaattag ctctgtcact tctgcctgtg ttagtgcagt accgtttgga
    21121 tcgtcgtagt aagctttagt tccttcgata atagcttttt taacgtcatc actagcatag
    21181 gtttttccgt tggcagtaat ggtcatatca ttgagtagaa agcgaagtcc gtcatttcct
    21241 agaaggagat ggacatcaga atatccgagc tcactggcct tgtctacgat gcgtttgagc
    21301 tggttcagag taaagtattt gcgtccagca tcgattgaga ttaccttgtt tttggcaagt
    21361 ttttcaacct cacgtttagc ttcttcttct ttttgagctt caggcgtgag ggtcaagttg
    21421 ttgacagttt cttgaagttt agcaatggct tgatcaatcg tatcttgttg ggcacggcta
    21481 aggttgctat cgagagagcg aatagctttt tcagcttctt ttacggccgc gacgctttct
    21541 gcagtataac ggttcaggtc ttttggtacc tcgttaagtg cttgctctgc agattcataa
    21601 tcagctgcga agtattcagc gttggcattt gcaaaatgac gcatgagttt gaagaggcgt
    21661 gatggtgaat aacgtgcaga tggagtgtca gcccaagcag ctaccatacc accgatgatt
    21721 gggatatcag ctccttctgt ttttggtaca gaagtgattg gtgtgttttt aataccattg
    21781 agcccctgat cgagattgta ccagccttgg ccatcagcgt ttcgtccaag aacgtagtac
    21841 caagcatcat tggtattaag gatttggtga cctttttcag ctagtagttt agaagaagcg
    21901 acatcgtagc ctccccaacc accagtccac atagaaacga tgatgtcttt gtcaaaacta
    21961 ccaaagcttg tgtcgctatt gtagtagata ccgtcgttaa aggccattgg tttgagaccg
    22021 tgcgatttta caatacgagc gaggtcattg gcgtaggcaa taaatttttc atagcctttt
    22081 acagggtagc cttcgtttgg atagtattta tcagcttgaa gcacactcca acctttagca
    22141 tctgtcgcat cattggcata ttcatcaagt ccgatgttga agatttcagt ctttttcgcg
    22201 aaataagcag catacttgtc gataagggct tttgtaaaag cgacagcttg ttcgttgtca
    22261 agatcgacag tacgggctga tttcttccca aaatagctaa agttagggtt ttggattccc
    22321 aattctttca tggcattgag aatcgcatcc atgtgtccag gactatttac tgtcggaatg
    22381 agaccgatac ctttatcttt ggcatagtta atcagatctg tcatttgact ttctgttaag
    22441 tgattgccgt ttggatcgtt gtaataatca tttgtacctt tttcaatggc gcgtttgaca
    22501 tcgtcactgg cataggtctt gccgttagct gtgatgctca tatcgtccaa catgaaacgg
    22561 agtccatcat ttccgactaa taggtgtaaa tcagtgtagc cataatgttt cgctttatcg
    22621 atgatttcct tgagctgttc tggtgagaaa tatttacgtc cagcatcaat agaaacaatt
    22681 ttctttttcg ctagtttttc atttacagtt gcagcacgtt cctttcctgc ctctgtttcc
    22741 ggtttgtcag cctctgcttt cgcttcatct tttttagctg gtttatcctt gtcagtcttg
    22801 tctgtatttg actctttaga atcaacctct ttcgcttctt cctttttagg gctagcttct
    22861 tctgcctttt tattagcagt ttctttttca gcagaagttg gagttaccac ttctgcttta
    22921 tcactaggag ttgaactaac ttcctcttgt ggtttttctt ctgtttttgg aagactagct
    22981 accttatcag tagctggagt ttctgtttct acagtttttg gagcttctgg ttgaagcact
    23041 gctttaggtg tttcctcagt ccgattttcg gatgattgag gggaatcaga aaccgtatgg
    23101 atggtcggtt ggttttctgt agtagtagga gtaactccat cggctgcaac agtctgtgct
    23161 tggaaggcaa atccaattag aacagaagct gctcctacag cgtatttacg aatagaaaaa
    23221 cgctgttgtt tttcatgttt cattgcaaaa cctcctgatt gcattgttat attgatagcg
    23281 attatataaa tcaacgccct tattttattt cttatattaa cgagagtcaa gaggagatga
    23341 caaaaaacta taataagtat aaaaaaatat aaaatttaaa cttaagattt cagattg
//
LOCUS       DAVKLW010000032        22347 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKLW010000032 DAVKLW010000000
VERSION     DAVKLW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.032"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(50..511)
                     /locus_tag="VW324_001238"
     CDS             complement(50..511)
                     /locus_tag="VW324_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9726879.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(478..621)
                     /locus_tag="VW324_001239"
     CDS             complement(478..621)
                     /locus_tag="VW324_001239"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU9726880.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            890..1270
                     /locus_tag="VW324_001240"
     CDS             890..1270
                     /locus_tag="VW324_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9726881.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1369..1590
                     /locus_tag="VW324_001241"
     CDS             1369..1590
                     /locus_tag="VW324_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9726882.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1607..1921
                     /gene="trxA"
                     /locus_tag="VW324_001242"
     CDS             1607..1921
                     /gene="trxA"
                     /locus_tag="VW324_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9726883.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2296..2760
                     /gene="queF"
                     /locus_tag="VW324_001243"
     CDS             2296..2760
                     /gene="queF"
                     /locus_tag="VW324_001243"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9726884.1"
                     /translation="MQHYETVEAVTFAYGQRHHLEIQITREIAKEQGIRHHILDMSLL
                     GQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLD
                     PRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR"
     gene            2822..3214
                     /locus_tag="VW324_001244"
     CDS             2822..3214
                     /locus_tag="VW324_001244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726885.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDEVSLKHCFEVADRTDEVSLK
                     HCFEVVDRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVS
                     NHTYDKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(3239..3907)
                     /locus_tag="VW324_001245"
     CDS             complement(3239..3907)
                     /locus_tag="VW324_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9726886.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(4018..4809)
                     /locus_tag="VW324_001246"
     CDS             complement(4018..4809)
                     /locus_tag="VW324_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9726887.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AIGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(5056..6852)
                     /gene="pepF"
                     /locus_tag="VW324_001247"
     CDS             complement(5056..6852)
                     /gene="pepF"
                     /locus_tag="VW324_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9726888.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(6863..7606)
                     /locus_tag="VW324_001248"
     CDS             complement(6863..7606)
                     /locus_tag="VW324_001248"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9726889.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGSKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7608..8558)
                     /locus_tag="VW324_001249"
     CDS             complement(7608..8558)
                     /locus_tag="VW324_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9726890.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNMD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(8696..9124)
                     /locus_tag="VW324_001250"
     CDS             complement(8696..9124)
                     /locus_tag="VW324_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9726891.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(9126..10193)
                     /locus_tag="VW324_001251"
     CDS             complement(9126..10193)
                     /locus_tag="VW324_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9726892.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            complement(10212..10682)
                     /locus_tag="VW324_001252"
     CDS             complement(10212..10682)
                     /locus_tag="VW324_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9726893.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(10973..11425)
                     /locus_tag="VW324_001253"
     CDS             complement(10973..11425)
                     /locus_tag="VW324_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9726894.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(11462..11635)
                     /locus_tag="VW324_001254"
     CDS             complement(11462..11635)
                     /locus_tag="VW324_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9726895.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            complement(11778..11957)
                     /locus_tag="VW324_001255"
     CDS             complement(11778..11957)
                     /locus_tag="VW324_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726896.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(12122..12360)
                     /locus_tag="VW324_001256"
                     /pseudo
     CDS             complement(12122..12360)
                     /locus_tag="VW324_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12630..13901
                     /locus_tag="VW324_001257"
     CDS             12630..13901
                     /locus_tag="VW324_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9726897.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKASGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            14165..14237
                     /locus_tag="VW324_001258"
     tRNA            14165..14237
                     /locus_tag="VW324_001258"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14198..14200,aa:Lys,seq:ctt)
     gene            complement(14447..15766)
                     /locus_tag="VW324_001259"
     CDS             complement(14447..15766)
                     /locus_tag="VW324_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9726898.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKNGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(15776..17392)
                     /locus_tag="VW324_001260"
     CDS             complement(15776..17392)
                     /locus_tag="VW324_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9726899.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            complement(17421..18311)
                     /locus_tag="VW324_001261"
     CDS             complement(17421..18311)
                     /locus_tag="VW324_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9726900.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            complement(18322..19239)
                     /locus_tag="VW324_001262"
     CDS             complement(18322..19239)
                     /locus_tag="VW324_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9726901.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            complement(19391..20392)
                     /locus_tag="VW324_001263"
     CDS             complement(19391..20392)
                     /locus_tag="VW324_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9726902.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            complement(20754..22235)
                     /locus_tag="VW324_001264"
     CDS             complement(20754..22235)
                     /locus_tag="VW324_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9726903.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
BASE COUNT         6802 a         4827 c         3905 g         6813 t
ORIGIN      
        1 atcagctaat ttttggttta cattcctatt tttatgcata attattctgt cattctctaa
       61 tttgtgaatc aatatatctg attagaaaac taccattgag tttttgctcc acctcattag
      121 tccgctcaaa acgccgtccg ctaatcttgt catagacttt ctcccctcta ggagcccctg
      181 cataaggacg atagaggtag cggttgattc ccgtttcgtc aatatatagg actggtaaat
      241 cttttaggtt atccaaaata tcaagaaact cagaaacttt ctcaggcttt tgttccctaa
      301 aactggtcgt cttttttaaa atgaccttaa tctgctttaa aactgcccat acggagggca
      361 aagcacaatc aaaacgggcc gcaatttccc gtaaaaaagc gtctggatga gcctctacaa
      421 aggctttcaa ttcttctaaa gggatcttac gctttttgac gactcgcttt ttccgctcta
      481 agttcccttg ctcacgtctt tcttttccca cgtgaagaga gttctgacgc cgacatcaaa
      541 aactttggcc gcctcgacat gtctgtgccc tcctttgatg gaatctaatg ctccttgttt
      601 aaaatcagta ctatatgcca tagctttatt agaccatgct tattttaaac taagcaacta
      661 tagatagaga aatgaaataa aatctgaatt cgaatgtgat aaggagttag aaattgaaac
      721 aataggattc ccccattatt cacacaaaaa taaaaccact aaaaaacaga gcttgccagc
      781 attcttgaaa agtagtataa ttattgcatg cgcaatcatc ttgtgacgca gagactgccc
      841 gttaatggac tttcttctat tttatgactc taaaaagaaa ggagacacta tgacctattt
      901 ggaaaaatgg tttgacttca atcggcgtca gaaagaaatt gaaagtctct tggaagagac
      961 cattgcccaa cagagcgagc aaagtctgac cttgaaagag ttctacctgc tctactatct
     1021 ggacttggct gaagaaaaat ctctacgcca gattgacctg ccagataagc ttcatctgag
     1081 cccgagcgca gtttctagga tggtggctcg cttggaagct aaaaattgcg gtctactcag
     1141 tcgcatgtgt tgccatcaag atagacgctc tagttttatc tgcctgacaa atgatgggca
     1201 aaagacactg gcctcgctac aaaagactgt cgaagaaagc ttggaagcag gtttggattt
     1261 cttgatttaa gatgattttt aaaaaagttg cgtgcgcaat catttttctt gacattctct
     1321 tttataagga gtaaaataaa gttagcatta aacaaaggag ttttaaatat gattgaaatt
     1381 acctatatag atgccagcaa gaacgaaaga actgtgactt tcgagtctta tgaagacttt
     1441 gaacgttcgc aacaagcttg ccttatcggc gtcgcagact actaccctgt ccaaaaatta
     1501 acctacaagg gtcataattt ggactaccat gggacttatg gagatatctt cttctatctc
     1561 atgaaacaag atttaagcca atataactaa aaaaaggaga aatacaatgg caaaagcaat
     1621 tacagatgca acattcgaac aagaaacaaa agacggtttg gtcttagtag acttctgggc
     1681 aacttggtgt ggtccatgtc gtatgcaagg tccaatcttg gacaaattgt ctgaagaact
     1741 ttcagaagat gtcttgaaaa tcgttaaaat ggacgttgat gaaaatccaa acacagctcg
     1801 tgcttttgga atcatgtcta ttccaactct tctcttcaaa aaagacggcc aagttgtcaa
     1861 acaagttgca ggtgttcaca cagcagaaca aatcaaggcc atcattgctg aattgagcta
     1921 atcaaacgag agaccaagta ctttctttgg tctctttttt cttgcccttt gccatttttc
     1981 aaaaaatatg ctagactata gtagattgaa actagaatag tacatctcta cttctaaaac
     2041 attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttcttatt tcactttact
     2101 atataaatag aatattacga tatttgggat atgccatcgc taaaaaaaac ctcgatttgg
     2161 tattttttag ctccctaatg ggagcttttt acgtgctctg aacatttccc attttggaag
     2221 gagtactatg aaacgtcaat cagccttggt cgtcttagtg gtggtcaaga ttcaaaaacc
     2281 tgccttttct ggaccatgca acactatgaa acagtcgaag ctgtcacctt tgcctacggc
     2341 caacgtcatc acctcgaaat tcaaattact agagaaatcg ctaaggaaca gggcattcgt
     2401 caccatatcc tcgatatgtc tctgctggga caaatcactg ctcagccaga ctttgcgacg
     2461 attcatattt cctacattcc tgacaagctc tgtgtcgagt caaaatccct caaactatat
     2521 ctatttagct accgaaacca cggagatttc cacgaaaact gtatcaacac catcgggaaa
     2581 gacttggtca acttgctaga ccctcgctat ttagaagtct ggggaaaatt cactccgcgc
     2641 ggtggcattt caatcgaccc ctactataac tacggtaagc aaggaactaa gtatgagggc
     2701 ttggcagaac aacgcctctt ccaacacgac ctttatccag agaaaattga caaccgctaa
     2761 agtcatactc aatgaaaatc aaagagcaaa ctaggaagtt agccgcaggt tactcaaagc
     2821 agtgtttgag gttgtagata aaactgacga agtcagctca aaacactgtt ttgaggttgc
     2881 agatagaact gacgaagtca gcttaaaaca ctgttttgag gttgcagata gaactgacga
     2941 agtcagctta aaacactgtt ttgaggttgt ggatagaact gacgaagtca gcttaaaaca
     3001 ctgttttgag gttgcagata gaactgacga agtcagctta aaacactgtt ttgaggttgc
     3061 agatagaact gacgaagtca gcttaaaaca ctgttttgag gttgcagata gaactgacga
     3121 agtcagtaac catacctacg acaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga
     3181 gtatcatacg aaaaagccct gttcctcaag atgaggagca aggctttttg agtttcaatt
     3241 attcttctgt tccaaggaaa ttttttgcaa caagggctgc aagaactcca ccagcgattg
     3301 gtgcaaggat gaaaatccaa acttgttgaa gggctgcgcc gcctaccaag acagctggtg
     3361 ccaagctacg agctgggttt actgaaagtc cagtaatctt caatccgaca agaatcatcg
     3421 ccatcaatga caaaccgatt accaaaccag caatcgggcc attgcccttg ctttctgaag
     3481 tcacagtcat gataaccaat acaaacaaga aagttgcgat gacttcaaac aagaaaccac
     3541 caaagacagt gacaccgttt gccaaggcat tttcaccaag actagcagtt gacatacctg
     3601 agttagccaa gaggaagaag acagcgccag aagcgatgaa agctccaaca acctgaccaa
     3661 ggatgtagtt tacaagttct gaagatgaca aacgtttgtt tacaaacata gcaatcgaaa
     3721 cagccgggtt caagtgagca cctgaaacag ttccgattga gtaggctgcc accacgattg
     3781 ccaaaccaaa ggcaaaggcg attccaaggt gaccaaggcc atcaaaacca tttccaaaaa
     3841 caacagctcc tgtcccaacg aacacaagca tgaacgtacc gattaactca gcaacaaatt
     3901 ttttcatttt ctttctcctt ttttcaaaaa ctagatacta gtctatcaaa agtaggaaag
     3961 ggtttcaaga aaattgattg gaaatttttt gaaaatcata gaactattag ctaatctcta
     4021 gtattgaaaa gactggatag cttctttcaa ctcatcttgt aaactatttc tctggtcaag
     4081 ttggacatag acttccacca gacaggatct aaagttggaa aatttggaaa aatcctccct
     4141 ttcttctatc ggaaaatcaa cagtttttat ccaagaagct acttgttctt gctccaactt
     4201 cccttgtaaa ataggttcat agatcactct tgctaaacgc caatcctcat catctgtaaa
     4261 gcgaatcgac attcttttaa atagttggcc aagtatgtca aatacttcat gaattctgtt
     4321 tataggaaag tctggatgac agaccacctc tgtcagtaaa tcggctccat gtgcaaaagc
     4381 atgaacccaa ccatactgac ttgagaaacc tgttgtatcc ttttcttttg aaagatagtg
     4441 caagccttga tttgaaaaga cattacgaat ttctaattgt aatccctgat aaaaaaccga
     4501 ttgctggttg gcatctgcat acaagagatt tgcataaaca agtgccctaa aagaacgttc
     4561 aagtgttggc agacctacct tatcaatctc tttatctagt cctacatcag atgaaacccc
     4621 ttcagcaatg aaatgaaatt gctcctgtgt aaatagctct tcctgaatcc ctatagcaaa
     4681 gcttgtaaaa acaaggtcat cgcgaatttc tggagaagga tctcccaaat gatcaagcaa
     4741 ccactggatt tcctcctggt tataatttgg tttttcttct gctatttttc ttagtaaatc
     4801 ttgatacatg gtcaatacct ctacatttct agcaactgtt caaaaaggca gtcttaaatg
     4861 actcaatatt gaattctcaa ttaaatacaa tctgatataa aatgacgtaa ataactatca
     4921 ataccagttc tacagtaagt tcaaatttaa catcacgacc ttcaacgaca tttttgaaaa
     4981 tagctacaac taagacaaat agaatgacgc ttaacaagcc cataaacatc attctaaaaa
     5041 atttttctat tccccttact cccccaactc agcactgtag gcgataatct ggtcaactgt
     5101 gtcagacaag aattggatgg tatcacggag tggtttgtct gttgaaatat cagcaccgat
     5161 aatcatggct gactcaagtg gtgtcttgct accacctgat ttgaggagat tgagccagtc
     5221 ttcagctcca gtttctgagt ttttaagatg aaggtaacca gctgtcgaga tgacaagacc
     5281 agcagagtaa gtgtaactat acaagcccat atagtagtga gcttggcgca tccaagtcaa
     5341 agctgcatcg tcgtcgattt caatggcatc gccccaaaaa tctgtcaaaa cttccttcat
     5401 aatactgttg agtttgcttg ctccgaaggt ttctccttct tcaatcaatg tatacacctt
     5461 acgctggaag gcggcttcca agaggtgggt gataaagtta tggaagtagg tgtctgtcaa
     5521 gcgatgagcc agagcaaagc gtttttgacg tgggtcatta gattggttct ctaagtaatc
     5581 actgagtagc aattcattga aggttgacgg tgcttcaaca tagtaggtcg acatatgggc
     5641 attgaagtaa ctttgatgat tgtctgaaaa gatgaattga ccagaatgcc cgatttcatg
     5701 aatcaaggta tagacatcgc tcaaacggcc tgtccagctc atgaggacat aagggtgtac
     5761 gcgatatggg tccgccgcat aaccaccgga atccttgcca ctgttagcag caaagtccac
     5821 ccagcgctct tcttggtaac gagcaacttc ctgacaatat tcttgcccca aaggttctac
     5881 cgacttcatg accaaatcat aggcatcgtc aatagtcact tcaggattca gggcgctgtc
     5941 caagtccaat ttccagtctg caaaggtcat cttttcaaga ccatttacct tggcaacatg
     6001 cttgaggtat ctctgagcga ctggtgcaaa gtccttcatg atgaggtcaa tctggcggtc
     6061 aaacatgaca cggtccactt cttgttcagc tagaagatag tcaaagacag agtcgtatcc
     6121 cttcatatca gccaagagtt tttcagactt gacctgagcc agataggctg ctgcagccgt
     6181 attttggtgc ttacgaagtc cctctgagaa ggaacggaag gatttctcac gaacctcagc
     6241 atcctcgtga ttttggtaga aattctcata ggtgacaaag ctgtttttat aggtcttgcc
     6301 atgggcttca aagtcagcca tttcaaaatc cccagctcgc atcttagtat aaatgtcctg
     6361 cggactatag aaaacttcac cgagattggt caaggccttc tccacatcag cccctagata
     6421 gtgggctttt ttgattttgg cctgacgaat agctgccgtc aagtgaggca attcacccaa
     6481 acggtccaag acttcctcgt ctgctaccac caaggcatcg tcaaagaagg tcaaggctac
     6541 gctggcatct gtttcaaatt ccatcccagc ttgggcaata ttggcaaatt cgtcattgct
     6601 atagtccgtc gtctgaggca taaaagcata gttgccaata tggctcatct gaatgtagat
     6661 ctgttccaat tccgcaaagg ccttctcgaa atcctcaaaa gtatgaagat tacccttgta
     6721 gtcacggcta aattggttga tgtcttcacg agctttctcg attgcacgca agaaatcctc
     6781 acggtcttgg tatagggctg ttaagtccca gagttccttc tctggaaatt ctgaacggtg
     6841 tttttgttcc attttcttcc tcttatttct ctaattctac taaaacacta agggctgata
     6901 aggcgtaaag cggtgctgtt tctgctcgca aaatacgagg acctaggcct gccaaaacgg
     6961 ctcctttagc ttcaaaactt tcgatttctg caggtgacag accaccttct ggaccaaaga
     7021 taaagagcag tttggatcct gtttcaagac cagtgactgc ttgcagaagc gcagcggctt
     7081 ctccttcttt agctgattct tcataggcta ctatgataga gtcaaactgg tccagctgag
     7141 ctagaaaatc tgcttttttc tcgaaaagtt taatacttgg tacaatatta cgcttgcttt
     7201 gctcggctgc tccaagggca attttttcta gtttttcaac ttttttaccc aatttcttgc
     7261 catcccactt ggcaactgac cagtctgcag gaaaggccca gatttggcta gcacccagtt
     7321 cggttacttt ttgagcgatg aactccagct tgtctccctt gggaaatcca gatgcgatgg
     7381 tcacttggac tggtagttcc acattgtcat ttaattcttg gaccaactca aactgacgat
     7441 tttccatatc cagcacgcgc gccaagcgct tgatgccatc atcaaagact aaggtaacct
     7501 catcctcttc tttcaagcgc ataacctgaa acatatgctt actggtttcc ttgtcctcga
     7561 tagtgacagg agagatagca ctgcctttta caaaatactg ctgcatgcta gcctccaatc
     7621 acaccagaga tatccttggt tttcttaaag acacaggtat tccattcccc ttgaaccatg
     7681 tgagtttcga ggaaaaatcc agctgattca gccgactcgc gcaccatgtc ccacttatcc
     7741 ttgataatgc cactcatgat caggtagcct tcgtccttaa ccaaacgata ggcatcgtct
     7801 attagatgaa tgaggatatc cgccaagata ttagccacaa tcacatctgc ctcaatttcc
     7861 acacccttaa gcaaatctcc agccgctaca tggatatttt ccatgccagg gttgagctca
     7921 atattttcct gagccacacg aaccgccaca tcatccaggt cataggcgaa aatttcttta
     7981 gcccccagaa gcgagctggc aatagagaga acccctgaac cagtccccac atctagcacc
     8041 gtttcgccac cacgaagaac ctgttccaag gcaaaaaggc tcatcttggt agttgggtgg
     8101 gttccagtac caaaagccat gccaggatcc agcttgataa tcatttcccc cgcagtcgcc
     8161 tcatagtctg tccaagaggg aacgatggtc aaatcatgag tgatacgagc tggctcatag
     8221 tatttcttcc agttgtctgc ccagtcttcc tcagccaagg cagtcgtacc tatttttaac
     8281 tctcccaaat ccataaaatc cgtcaattct gctaagcgag cctgcaagtc cgcctcaacc
     8341 actgtcacat ccaccgtgtc agggtagtag gctgtcacta cgatttcttc ttgctgctcc
     8401 acctctggga aaatctcgcc aaagcggtcc atatttccca catagtccat gctgtcttca
     8461 atcgcaacac cttgcgctcc tagctcaatc aagagattgg aaaccaactc ctctccctca
     8521 cgcttcactg taacttttaa ctcttgccat gtttccatta ttaagatacc aagcccgtaa
     8581 aacacaaaac caaaatagga aattctctga agacgtttgt gtctaagaga agtttatctt
     8641 tttggcaccg tgtttagggc gggttcagtt tagaaattta accgaaccat cctttctaat
     8701 cacttacttt taaataatct tttaatctct cttgcaactg aggcacaact tgactggaac
     8761 taagaaattc ctcaacattc atcagctgat agccctgtcc ttcatctcca aatacgatac
     8821 tgtcaaactg gttctgtgtc aactgaccaa ctagaaaaac ggactgtttg tctgcaaaaa
     8881 gcatacttgg gtaaatctta ctccaaagca gacaatcttc agtcagatga atgcccagtt
     8941 cttcataaac ttcacgcgct acacactcaa aaggactttc atcaccttca cgtccaccac
     9001 ctggcagttc ccacatattg gcccagggaa tgcttgcctt atcatcgcgt aagatagtca
     9061 aaagcttatc cccacaaaac aaagcaatct tgcaacctgt gaaatcagaa atctctattt
     9121 ccatattaga ctccttcggc taattccttt tccagctctg ctaacaagtt ttcataatag
     9181 cgtttctcgt ccctcaacat tcgactacta tccattttct gtctagcaat cttgagagcc
     9241 ttacgagttc gatctacatc tttcttcacc tttaattgat accaggcttg tatcacttga
     9301 agattggaca gtttgagaga cagaaacgat ttgacctgtc gaatactggc atattgctcc
     9361 gcttgctcaa aatctccttc caacaaggcg atatgaagca gggatagttg ggcaactgtc
     9421 tgcatcatcg gagtagttgt cctctcaagt aatgcttgaa actgctgttt agctacttct
     9481 tccttccctt ccaaaatgga aacttcacct tgcataccta atacaccatc cgcaaaactc
     9541 cctcgtgcat cctcaggaac tgcttgaaca aagtctttca aatcatattc ttgaggagcc
     9601 agcaaagttt gagcagaatg tctcaatacc aggtaggcgt atttggtatt ttcagggtgt
     9661 tgtagtaatt cccaaatttt tgctccatcg gtgatgtcga ctggcaaaat gttatttagg
     9721 aagaaagata aattaagaaa aatccaagtc cctgcaaaat accagcttct tgtcaaaaat
     9781 ccaaacacta tcgccaataa tatcaagccg agatgaacca tcaagcctcc tgaaagcatc
     9841 aggatgattc tttgatcgct ttcatcctct tttaaaccaa tgtattgagc accaacattt
     9901 ttcagaatgg ctgttctact aagatgaaac ctgcctgact ttttggtcaa aataaaatgt
     9961 cctaatccaa aagccaccag ccgatagcct gtcaagtagc cacaaaaagc atgacccagc
    10021 tcatgaagaa taaagattaa atacatgctt agaagagcga aggcataacc aaaagtaaag
    10081 gctaaaactg cggaataccc caactctgca aatgcgattg ttccacaagc aaaagctagc
    10141 ataataaaga caacagctag cacataaacc aaataagtcc caattttctt cataacacct
    10201 ccaaccaact cctagtatct tggataagga taaaattctc cctcttccaa gccaactttt
    10261 ccttcttcaa agacttcttg attccattcc ataacaaatt cctctgcttc tggatcttcc
    10321 aaaaagtcca tgagggtatc tagcccaacc tccgcagtat ctttgaggaa aagggcaaaa
    10381 taagctaaaa actcgcgaga aaatcctttt ttaggtagat aagggataac cgttaaatag
    10441 tcttctgcat tgacggttga cttggcagga ttgtagaaaa ggaccgcttc ctcaaaaaga
    10501 atgtcatctg atgaaacctc tccgtcttca tccaccatct ccacacctgc agcattttgc
    10561 gcttccaata gaaaactcac ttctaccgcg tggttacgct tgtcccaact aatctcaaag
    10621 tcaaagggaa agttcttgtc caactcttcc tctaaaatat ctaaaaatcc gtatgttgcc
    10681 attttgtcct ctttctatgc gactctttaa tcgccccgat tgctcggaaa tatgctaaaa
    10741 tagatactac catcttacca caaaattatt ttatgtccta attataccat attacctcat
    10801 ttaaaccctt ggtatcagtg attttcttaa aagtctgatt tcttcatttc tcataaaaat
    10861 caatataaaa agccctcgaa agggctaata aatctataaa atcaataggc gagtaactag
    10921 cacaagtgga cgtgcttttt tattgactat taccacgata ccacgcttaa tcttaggctt
    10981 gaactttctt atctgcaata gcgtctgtca aagtctgaga aaagttaagt cccatttctc
    11041 gtcccaactt atctgcccat tttggtatgg tcaaagtctt tttaatgggt tcctgacttc
    11101 ctaggtattc tgatacatca acagatacca tagaaataaa agatttatca aggtcatagg
    11161 ttgacacgaa atcttcatca tctttaaaag gatcattatc aattaaagac aagctattga
    11221 tatctgatgg ctgaggtaac tctccatcac tctctatcaa atctgcaaca gttatcccta
    11281 gccactccga ccccatagcc aaagcctcag aaatcccctc tccttgtgta gctgagtatt
    11341 caaaatctgg gaaatggaca aaataagtcg cttctgttcc gtctgtgtcg tcataataaa
    11401 ataaagctgg atacgtaact aacatttcac tacctccata tcaaaaagca gggactgaat
    11461 tttacaaccc agcttgcttt cctatccctc tttcagtgta cttattcagc tcaccatgaa
    11521 ggattgtgat aggtctttcc ccttgcttct cgattttaat atgggagcct ttaccgcctc
    11581 tagtctttat ccaaccatgg gccgtaagga gtttaaccat ctctttttgt gtcataggta
    11641 tagcgctttt acctcctgac aacaccatta taacacgtgt tacacgtatt gtaaaggagt
    11701 gatacttatt attctattat acataaaagc ccctagatgt ggttctaagg gaaaccaatt
    11761 tattcatacc tatttttcta atgagtagta aaaactgctt ctttatcgag caattcatca
    11821 tctgtatagt caattgtaaa agtatctcga tctaagacag attgaggcgg agttgaatga
    11881 atcataggac cactgcgtac tctatatttt ttatctccaa tttttacaaa ctgatactct
    11941 tcgaaaatca aattcaaacc acgtcaacgt cgccttaccg tactcaagta cagcctgcgg
    12001 ctagtttcct agtttgctct ttgattttca ttgagtatga ttaactctca agtcttcgaa
    12061 atcaggattt tcaacagtta ttacaaggag gcgatttact acttcaaaaa catcaattat
    12121 tctatttttc atattttttc aacccattat tagaatgaac ttcttggtaa gcaaaatcaa
    12181 gtttagattt aatgttttcg tacaaatcta aaatctcttt tggagtatct tccggaagaa
    12241 aagttttctt ttccctgaaa taacttgatc actaagaatc caatgacgaa tttgttttgt
    12301 aaaaatcaaa atttcctgac ttggtccatc atttccattg cttatcacct ctcttttcat
    12361 tatagttcat acaatgacat tcagcaatat tatttctcaa gtcagcactt ccacttcttt
    12421 aggctcaact atcctatttt gagctttaag gaaaatcaaa tctctcatgc tgatacctct
    12481 cctcattaaa ttaaatagta aaaaagattc tatctcactc cctgattatt acaaaaccat
    12541 tgaaatatca caactaatag gctagaatgg acatagtaag atatagtaga tgagtcattc
    12601 tactcaaatc cacgctagaa aggactgcta tgccagacaa tctcgcgctt cgcatgcgcc
    12661 ctaaaaccat cgaccaggtc atcggtcagg agcatctggt cggacctgga aaaatcatcc
    12721 gccgcatggt ggaagccaac cgcctgtcct ccatgattct atatggccct ccgggaatcg
    12781 gcaaaaccag tattgcctct gccatcgctg gaacgaccaa gtatgccttt cgaactttca
    12841 atgcgacagt tgatagtaaa aagagactgc aagaaatctc ggaagaagct aaattttctg
    12901 gtggtctcgt cctattgcta gacgaaattc atcgactaga taagaccaag caagacttcc
    12961 tcttgcctct cttggaaagt ggactggtca tcatgattgg agcaacgact gaaaatcctt
    13021 tcttttccgt cactcctgcc attcgtagcc gtgttcaaat ttttgagttg gaacctctgt
    13081 ctaaccaaga cgtcaaagag gccctgcaga tagctctaag taaccctgaa cgtggttttg
    13141 attttccaat agaactagat gaggatgcgc tggatttcat cgcaacctct acaaacggag
    13201 atcttcgctc tgcctttaac tcactggact tggctgttct ctctacccct gagaatgacg
    13261 agggcattcg ccacatcacc ttagacatca tggaaaatag tcttcagaga agctacatca
    13321 ctatggacaa ggatggagac ggtcactatg atgttctatc tgccctgcaa aagtctatcc
    13381 gtggctcaga tgtggatgcc agtctccact atgctgcccg cttgattgag gctggggatc
    13441 tgcctagtct cgctcgtcgc ttgactgtta tcgcctatga agatatcggt ttagccaatc
    13501 ctgaggccca gattcatacc gtgactgctc tggatgctgc acaaaggatt ggtttcccag
    13561 aagcccgcat tctcatcgcc aatgtcgtga ttgatttggc cctttctcca aaatccaact
    13621 cagcctatgt agctatggat aaggcacttg ctgacctcaa agcatcaggg cacttgccta
    13681 ttccgcgaca cctgcgtgat gggcactaca gtggaagcaa ggaactgggg aatgcccaag
    13741 actatctcta tccacacaac tatcctggaa attgggtcaa gcaagactat ctgccagaaa
    13801 aaattcgtaa tcatcactat ttccaagcag aagatactgg taaatatgaa cgggctttgg
    13861 ctcaaagaaa ggaagctatc gaccatttgc gaaaaatctg aaatcctttt caaaaaactg
    13921 cactttcctc ttgatttttt ttgaaaaagt ggtatcatat aaatatagaa acgctgtggt
    13981 gtacgacttc acacttaagt gttgaccgac tattttttgt attattaggg aaacaaaagt
    14041 cttctaacag catgtaggcc gtctcacacg gaaacagctt cagttagagc gagttgccca
    14101 cctgcttaat tgcgcgggtt caatacaaac cgtgaagttt cggcaccaat acagcttttt
    14161 atttgccttc ttagctcagt tggtagagca gtagactctt aatctatggg tcgcaggttc
    14221 gagccctgca gggggcatct aaatcaacag gaaaaagcct tataatatag ggcttttttt
    14281 gcttaaataa aatttttttc aaattcttta gaaaactagt ttaaatcata atccaacacc
    14341 aattttacaa ataccataaa ctaagcttat atacccaaaa cacaaaaagt cctctttcca
    14401 ctattttaga aagagaactt tttctttagg agaaataact taatttttaa tcaattcgtg
    14461 atagctcagc tggcaccgtt aagtaataag ttgcagtcaa gcttgcttca ccttgattga
    14521 caaaaatctc gatggaattt ttatctagaa caacttccaa ttctttagct tcaatatcta
    14581 cataccgtcg actagtgtcc tgttcttctt cacctagaat tttttgaata agatggctac
    14641 gatcaatgta aacttgctgc gcattactgt cataaccaaa ttcaagataa tctgtatcat
    14701 ttcctaagtg gtaatgacaa tctttatcta tttggatttg atattggcct tttttaacag
    14761 ggaattgtct tagtttgcca tcttccaatc ttagaattct aggtaaagtc atggcacatg
    14821 cccacttgtg ttcttggtca tgggttggaa gggtacgccc ccatgtctgc atccaagcaa
    14881 tcaggatacg acgattttga tcgtccaaca atgtttgagg cgcatagaag tcttggccat
    14941 gatcaatttc ttgaactgat tctgggataa aacatttttc tccccactct accttacccg
    15001 tgaacaaaag cgatgagttg atgttatgat atgagtctcc ctcacgctga taacgcatgg
    15061 gtgacataat aaggcaatct ttcccatcta actcgaagta atctgggcat tcccacataa
    15121 acccttggtg ttctacccct tttaaaaaga tggattcgaa ctgccattct actaggttat
    15181 cggaccctag tagaacgata cagcccacat tatccttgtg tttggcagct actacggagt
    15241 aatagcgtcc atctttttca aagagttttg gatcacggaa atcagcagca atcaactcat
    15301 ctggcaagtc tgctcctgtt gcaactggat tttgttcaat cttttcaaag tgaatcccgt
    15361 cgtctgaaaa tgccatattt tgcacttggc ggacaccgtt tttttcttcg atatgcccag
    15421 tgtacatgag ccagaggcga tcatccttga caatggcaga gcctgagaaa caaccatttc
    15481 ggtcataatc ttgatcagga gcaagtgcca ctggcaagtg ctcccaagtc accaagtcct
    15541 tacttttagc atgtccccag tgcataggtc cccaaacact atcatatgga tagaattgat
    15601 aaaagagatg gtattctcca cgaaaataaa caaatccatt tggatcattg atccaaccaa
    15661 tctcagctga aaaatgttct tcaggcttat attgagtatt aacgagatgt ttattttctg
    15721 ctataaaacg attggctttt tctactgtgt atttcttatc tcccataatc aacctctagt
    15781 ttttgtttgc ttggtattgg tcgtagtatt tttgtttaat agcgaggtaa tcagaaagtc
    15841 cgtatttttc aagttctttc ttgtaatcat cccactcagt atcaatattg ccatttacaa
    15901 tccattcagc acgtttacgg tagatatagt cattcatatc tgcttcgata tgggcaatct
    15961 tgtccaaatc ttcctgtgtc ataaagactc ttggatagtt attgtcattg ctcatgtaag
    16021 gaacataata ttctttgata agatccaaac gccatttggc atcatcaggc atggttgtta
    16081 ctttaccata gtatgaatct aggatagcta gtggtcctcc tacttcagtc ttttgacgaa
    16141 gttctgctgg tgcagttccg tttagtggta agtgttttag actatttgac gcttgatcca
    16201 attcaaagat gttttgttgt ttgtcatctc cgtaagttcc ccagttattt tgcacagatt
    16261 ggagtggagc gtattgtgca tcaatccatt tagctgtcaa ttctaggttt ttgtttacac
    16321 tggtaataac catcttgtca cgtgcaaatc ccataccgtt tgtacgagct acgtgttttt
    16381 gaccacttgg tccagcaagt actggtaaaa catcataact ttcgttactt ccagtaacat
    16441 tattcttatc ccatgtaaag taaacaccaa atttctgatc atgaccttta gcaatgtaac
    16501 tattccaatc atgttcgaaa gcttctttat caatcaagcc tttttcttgc aattgacgga
    16561 taaatttgac accttcttta tagttatcgt tatctgctgt gaaatcaact ttgccatcat
    16621 ttcctactac taaatgatca tcgttatccc ctataccaaa tgcagcaaat aggaatttaa
    16681 aatcttcgtt tccgttacca ctaataaatg aaaatggaat ttcatcagcc tctccatttc
    16741 catttggatc cccgtttttg aaagcttcta ggactttaat caaatcatca gtagtttttg
    16801 gcatttcaag accaagtttc ttaagccaat ctttgttaat ccaagccata tcgttgacgc
    16861 tgtgaataga ctctttacca tctccaagct cttcaatcca tgggaatgag taaatgtgcc
    16921 catcaggtgc tgtcatcaag gccttgtact ctggtttctc atccaaaatt ttcttaagat
    16981 ttggcatgta tttatcaatc aaatcttcaa ctggaataat aacacctttt ttagcccagt
    17041 tcatcaagtc cacatctgaa gctccgtcgt tgtggatagc atctggtaaa tcaccactag
    17101 aaatatccaa gttacgtttt tctgcaaagt cggattggta gttggtccag tcaatatgaa
    17161 cgccagtttc cttctccaaa cgttgcaaaa ttaacttttc atttgggtct ttaggagata
    17221 acggtgaact ggctgtcata aacttcaatg ttttcttttc ttgaagcggg aatgttacac
    17281 cttccaactt ataatctgga cttgaagctg tattttttga gccacaggct gcaagtactg
    17341 ctaaactagc tgtcaacaaa actgctgatt ttgagaatgt tttgaatttc atgagaaaac
    17401 tccttttatt ttttcttttt ttatccttta agtgaaccag ccataattcc tttatcaaag
    17461 tatttttgga agaatggata cataacaatc aatggcaagc tggaaatgac aatagttgcg
    17521 tatttaatca attcagctaa acgtttcatt tcattcatag ccgcttgtgc tccaatcatg
    17581 tcttgacctg gttggctctg aatgagaatt ttacgaagta caagttgcaa tggttccaag
    17641 tttggatcct tgatataaat cattgcatca aagtatgagt tccactgtcc tacaaaagca
    17701 taaaggaaga gaacaaacat aattggtttt gcaagaggaa gcatgatttt gaagaaaatc
    17761 tgtaaatcat ttgcaccatc aatgacagca gcttcaacta attcttcagg caatccttgg
    17821 aaataggccc tagcaagaat aatattccaa acattgacag cacctggaac aatgatagcc
    17881 catggagtat tgagcattcc caattctttt acgagcaagt aagttgggac taaaccacca
    17941 ccaaagaaca tagttacaat caagaagtag ttaatccaac gacgtccaac caagtctttc
    18001 ttagaaagag gataagctgt aaacacagat agcaagactg ttaaagctgc aaatccaaaa
    18061 gagtatagta gagaattgat aaaacctctt agaatagatt ggtcactgaa tacacgctgg
    18121 taaccttcta cagtccaatc ggctggatta aagctaatcc ctctactaac cagaacctta
    18181 ggatccataa aggatgctac gacgatataa agtaaaggaa gcaaagtcat caaaacgata
    18241 aagacaataa tgattttatt taagagtaag atacgtctat caaattttgt atccataatc
    18301 gaatttttca tactttcctc cttaaattcc ttcaccatta ttcatgcgtt taacgatttg
    18361 gttaactgca acaagcaata ctacgttaat cactgcatta aacaaaccaa ccgctgttga
    18421 gtaagaatag tctcctgata caagaccaac tttatagaca tatgtcgaga taatttcaga
    18481 agttggcaaa tttaacgatg tctgcatcaa gaatgctttt tcatatccga cattcataat
    18541 tccacctgca gctaaaacaa attggataac cataataggc ttaagagctg gaatatcaat
    18601 gtgccagatt cgttggaaga tattggctcc atccagtcga gctgcttcta ctaaggctgg
    18661 atctacattt accaatgttg ccgtgtagag cgttgaagcc cagcccattc cttgccagat
    18721 accactaaag atgtataagg gtctaaagaa gtctggattt gtcaagaagt cagccttcat
    18781 tccaaacata gaaagaaaat tgttgattgg tcctcccact gaaaagaaga ggaaaatcat
    18841 accgacaata acaacgactg agataaagtt tggtgcgtat aaaatgagct gaattcgttt
    18901 tttgactttt tcactcaaga gttgattgag cataatcgca agaatgattg gtggtaaaaa
    18961 gccaagcaat aaaccataga tacttaattt taatgtgttg gctaacaaga taccaaagtt
    19021 gggagaggat atgaattttg taaactcaga aaaaccaatc caatcactcc ctaaaattcc
    19081 ttttaaagga ttgtaatctt taaatgcgat taaaacccca tacataggga tgtatttaaa
    19141 gataaaggtt aaaactaaac cggggacagt catcaataac aagagttttt gtttcttgat
    19201 tctttcccaa agagaaactt gcttcgcttt gctattcata gcaacctcct tttttttgaa
    19261 acgtttcatt ttttctataa aagatagcgc ttggcgctgt ctttttattt ctttttttga
    19321 aacgtttcat ttttgtttta aaaataaatc tttttatctg aattcattct atcattgata
    19381 gcgctttctg tcaagactta ttttcatttt ttgttgtttt tccttcaaca aattttactg
    19441 gtaggattgt ctgcaaatta acagtctttc catcaattat atcaaacaaa atacgaaccg
    19501 cttcttttgc catttcttcc aatggttgtt caatagttga aagtagataa tctctatctc
    19561 cagccatttt aattccatcg tatccaataa tttggacatc atgaggaata cgtttgccat
    19621 ttttttctag tatttcaatc acatccaaag ctgtaaaatc gttgattgta aaaattccat
    19681 ctatagaaga attttcttcc aagaaactct ctagctttcc ataatagtca gtaactgtct
    19741 catccaaatc aaaaatacta agaggtattt tatgctcttg gacatacttt tcaaatgaaa
    19801 ttctacgacg tttggtttca ttaatggttg tattatgtcc gcccacaaag gctaaatgct
    19861 gacatccttt actaattaat tgtttagctg cttctcttcc acctgcatca ttatctgatg
    19921 aaacacaagg aatggcaata tctgagtatg tgcggtcaat actaacaaag ggaattcctg
    19981 acgtcaagta atgttcaatt ggactatagg taatggcaac cactccatca actttattat
    20041 gacgcaacat ctccagatag tcttgctctc tatttgtacc atttatagaa cataagagta
    20101 atttgttatt tctcttatag acttcatttt ccacatgcat agcaaattct gaaaagaagg
    20161 gatgccagat acttggtaca atgattgcaa tcgtttctgt tcgatttttt ttcattcctc
    20221 tagcatagta atctggaatg taattcaaag ttttaatcgc ttgttccact tttttcaaag
    20281 ttacttcttt aatgcctttt tctttattaa ttacacgtga aacagttcca acactaactc
    20341 ctgcttctaa agcaacatct ttcatggtaa ttgattttct ttgttctacc atattatcac
    20401 ctcctttcaa tatatagtat catgcaaatg ctttttaagc aactatttct caatcatttt
    20461 tggccagatc atttatccca tcatgaataa aatcactcca attagctttt gaaaatactt
    20521 caattttcat gtgtaaacat ctacataaaa caggaaaagc cttggtttta tggctttttt
    20581 cgtatcttct acaaaaaaag caagagtttt agagggctat aaatctagat gtacattttg
    20641 cttaaatgat tgaaggtctt ttcttaacaa aaacacccca aaaattagat tttttctgtc
    20701 taacttttga ggtacagttc aaacgcgaaa tagcgttttt ttgttatttt tggttactca
    20761 tctaatcgaa taaacatcat ggcatttaac aagtatatga gtgagaccgt gtttatatta
    20821 tttgaataga tgagtctctt attttcaata ggaggaataa taaaattaga aataatgata
    20881 tcataaggtg aatcttctaa agattccttt gataattcta attcagtcca aacttccagt
    20941 tcaaaattat tgctacagta ataagaaagt gtctctgcaa cgaattttgc atgatactga
    21001 tcaaaattac tcataactaa aacctttagt ttaggctgat tttgtagcaa attaatcacc
    21061 aaatgtttgg tatgagtgat gaaggtataa gatagatgat ttaccatcat tgaactagaa
    21121 caaacctcaa gagtctctaa atagtgagaa agctcttttt ttatatctga aacaaatttt
    21181 ggaaaaatat tttgaaagtt cctgattgta ttcccttttt gatcaaataa aataaactca
    21241 gtaaacaact cttgacgata cagatgtgcg gtattatgca gatgccaaat cagattatcc
    21301 ttattctcca tttcaatctg atacttgact gaaatctgat caataaaatc actcaataga
    21361 tggtaagatt tttcaacata actatccttt tttacgcatt tcataaagag actttcatct
    21421 atgaaaaaca ttttttgaaa gtaagacaca aataattggc aaacaacttc ttcatctaaa
    21481 gagatattgt attctgattc aaaactctga gcaacacctt ctattccttc tgcctgcatt
    21541 aaaaaatcca aactttggtc gttaaaagaa tctttatcta cttccataaa atgaccaaac
    21601 tttattctat ataggttcgt aactaggagc aactttagca ttctatgcgt tgacaaattc
    21661 attggaaagc ttgtttcctt ataaaccaat tctaacaatt gagatagtgg ctctgatgaa
    21721 aaattttcaa atggccattc taggaaataa tatttttctg aaaaatattg tgcaaaaaag
    21781 taacgaatgt ctctctcatt tccaatgatt tgaacagggg tcagactgac ttcaaattga
    21841 aattgccttt taatcacttt attgatttgg ctaataatac gatagagcga agatgaactg
    21901 atataaaatt ctttacaaat actctcagct tgacaacctt cattaaagaa gatgaattct
    21961 aaaatcgaaa aatgagttga atgtttaaag aaatgatggt aaaccatttc aatatcacta
    22021 tcatcggtat taataatgcg tataccatta gtagaagaat gaaaaatcaa gtcaggaaaa
    22081 gcagatttaa catgggatag atcatctttg actgcacgtt ctgtacaatt taataactct
    22141 gctagttcag aacgatgaaa ccaacgttta tgttcaaata ataattctaa taattctaat
    22201 tgcctatgac tttttttaga taataaatct ctcatgaata tctttctctc tttataaatt
    22261 atcggattaa acctcttgca attataccac aaagaatagg tatagcatga tataacgact
    22321 tttcctaaaa tcttttattt cgtataa
//
LOCUS       DAVKLW010000033        22087 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKLW010000033 DAVKLW010000000
VERSION     DAVKLW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.033"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..267
                     /locus_tag="VW324_001265"
     CDS             1..267
                     /locus_tag="VW324_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEU9726904.1"
                     /translation="MLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAEL
                     YSLEKNEDASLSKLQADGRITEEQAKAYKEYHDKNGVANRKVND"
     gene            260..664
                     /locus_tag="VW324_001266"
     CDS             260..664
                     /locus_tag="VW324_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9726905.1"
                     /translation="MIKAFTMLESLLALSLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            669..929
                     /locus_tag="VW324_001267"
     CDS             669..929
                     /locus_tag="VW324_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726906.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            892..1353
                     /locus_tag="VW324_001268"
     CDS             892..1353
                     /locus_tag="VW324_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9726907.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRFYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            1331..1744
                     /locus_tag="VW324_001269"
     CDS             1331..1744
                     /locus_tag="VW324_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9726908.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            1881..2474
                     /locus_tag="VW324_001270"
                     /pseudo
     CDS             1881..2474
                     /locus_tag="VW324_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            2535..3488
                     /locus_tag="VW324_001271"
     CDS             2535..3488
                     /locus_tag="VW324_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9726909.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGETEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            3539..4729
                     /locus_tag="VW324_001272"
     CDS             3539..4729
                     /locus_tag="VW324_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9726910.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            4878..5249
                     /gene="rnpA"
                     /locus_tag="VW324_001273"
     CDS             4878..5249
                     /gene="rnpA"
                     /locus_tag="VW324_001273"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9726911.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            5224..6048
                     /locus_tag="VW324_001274"
     CDS             5224..6048
                     /locus_tag="VW324_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9726912.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            6067..7053
                     /locus_tag="VW324_001275"
     CDS             6067..7053
                     /locus_tag="VW324_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9726913.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            7104..7727
                     /locus_tag="VW324_001276"
     CDS             7104..7727
                     /locus_tag="VW324_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9726914.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQCLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            7868..9325
                     /locus_tag="VW324_001277"
     CDS             7868..9325
                     /locus_tag="VW324_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9726915.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDREKYYNGEVQEEA"
     gene            9348..9629
                     /locus_tag="VW324_001278"
     CDS             9348..9629
                     /locus_tag="VW324_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9726916.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            9705..10190
                     /locus_tag="VW324_001279"
     CDS             9705..10190
                     /locus_tag="VW324_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9726917.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            10207..10872
                     /locus_tag="VW324_001280"
     CDS             10207..10872
                     /locus_tag="VW324_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9726918.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            10876..11739
                     /locus_tag="VW324_001281"
     CDS             10876..11739
                     /locus_tag="VW324_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9726919.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            11741..12445
                     /locus_tag="VW324_001282"
     CDS             11741..12445
                     /locus_tag="VW324_001282"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9726920.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            12622..14295
                     /locus_tag="VW324_001283"
     CDS             12622..14295
                     /locus_tag="VW324_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9726921.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            14407..15498
                     /gene="ulaG"
                     /locus_tag="VW324_001284"
     CDS             14407..15498
                     /gene="ulaG"
                     /locus_tag="VW324_001284"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9726922.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNSKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            15612..17588
                     /gene="tkt"
                     /locus_tag="VW324_001285"
     CDS             15612..17588
                     /gene="tkt"
                     /locus_tag="VW324_001285"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9726923.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            17705..18004
                     /gene="yajC"
                     /locus_tag="VW324_001286"
     CDS             17705..18004
                     /gene="yajC"
                     /locus_tag="VW324_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9726924.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            18050..18478
                     /locus_tag="VW324_001287"
     CDS             18050..18478
                     /locus_tag="VW324_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9726925.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            18480..18890
                     /locus_tag="VW324_001288"
     CDS             18480..18890
                     /locus_tag="VW324_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726926.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            19190..21841
                     /gene="adhE"
                     /locus_tag="VW324_001289"
                     /gene_synonym="adhC"
     CDS             19190..21841
                     /gene="adhE"
                     /locus_tag="VW324_001289"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9726927.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELSFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6934 a         3976 c         4839 g         6338 t
ORIGIN      
        1 atgttggtgg tcttgctgat tatcagcgtg cttttcttgc tctttgtacc taatctgacc
       61 aagcaaaaag aggcagtcaa tgacaaagga aaagcagctg ttgttaaggt ggtggaaagc
      121 caggcagaac tttatagctt ggaaaagaat gaagatgcta gcctaagcaa gttacaagca
      181 gatgggcgaa tcacggaaga acaggctaaa gcttataaag aataccatga taaaaatgga
      241 gtagcaaatc gtaaagtcaa tgattaaggc ctttaccatg ctggaaagtc tcttggcttt
      301 gagtcttgtg agtatccttg ccttgggctt gtccggctct gttcagtcca cttttgcggc
      361 agtagaggaa cagattttct ttatggagtt tgaagaactc tatcgggaaa cccaaaaacg
      421 cagtgtagcc agtcagcaaa agactagtct gaacttagat gggcagacgc ttagcaatgg
      481 cagtcaaaag ttgccagtcc ctaaaggaat tcaggcccca tcaggtcaaa gtattacatt
      541 tgaccgtgct gggggcaatt cgtccctggc taaggttgaa tttcagacca gtaaaggagc
      601 gattcgctat caattatatc taggaaatgg aaaaattaaa cgcattaagg aaacaaaaaa
      661 ttagggcagt gattttactg gaagcagtag tcgctctagc tatctttgcc agcattgcga
      721 ccctcctttt gggacaaatt caaaaaaata ggcaagagga agcaaaaatc ttgcaaaagg
      781 aagaagtctt gagggtagct aagatggccc tgcagacggg gcaaaatcag gtaagcatca
      841 acggagttga gattcaggta ttttctagtg aaaaaggatt ggaggtctac catggttcag
      901 aacagttgtt ggcaatcaaa gagccataag gtcaaggctt ttaccttgtt agaatccctg
      961 cttgccctca ttgtcatcag tgggggatta ctcctttttc aagctatgag tcagctcctc
     1021 atttcagaag ttcgctacca gcaacaaagc gagcaaaagg agtggctctt gtttgtggac
     1081 caacttgagg tagaattaga ccgttcgcag ttcgaaaaag tggaaggcaa tcgtttctat
     1141 atgaagcaag atggcaagga catcgccatc ggtaagtcaa agtcagacga ttttcgtaaa
     1201 accgatgcta gtggacgggg gtatcagcct atggtttatg gcctcaaatc agctcagatt
     1261 acagaggaca atcaactggt tcattttcgt ttccagtttc aaaaaggctt agaaagggag
     1321 ttcatctatc gtgtggaaaa agaagaaagt taaggcaggt gttctcctct atgcagtcac
     1381 catggcagct atctttagtc ttttgttgca attttacttg aaccgacaag tcgcccacta
     1441 tcaagactat gctttgaata aagaaaaatt ggttgctttt gccatggcta aacgaaccaa
     1501 ggataaagcc gagcaagaaa gtggggaaca ggtctttaat ctaggtcagg taagctatca
     1561 aaacaagaaa acaagcttgg tgacgactgt tcgtacggat aagagtcaat atgagtttct
     1621 gtttccttca gtcaaaatca aagaagagaa aagagataaa aaggaagagg tagcgactga
     1681 ttcaagcgaa aaagcggaga agaaaaaatc agaagagaag cctgaaaaga aagagaattc
     1741 ctagtcaatt caactataat gcgttgaatc caaaatagtc cactgtagtt tctagaaaat
     1801 tgctggaaat ggatgttaag ctccaattca tttgtttata tcttatttca gtttactata
     1861 ctttgtgcta aactaaagat atgaaacatg attttaacca caaagcagaa actttcgatt
     1921 cccctaaaaa atcttcctcg caaacttggt atgtcaagca gccgagaaac agattgatct
     1981 tctatcagac aaagaaattt tagatttcgg tggtggcacg gatctattag ccttgcccct
     2041 agcccctagc caagcaggct aagtcagtca ctcttgtaga catttctgag aaaatgttgg
     2101 agcaagctcg tttgaaagtg gagcagcaag caatcaagaa tatccagttt ttggagcaag
     2161 atttaccgaa aaatcccttg gagaaagagt ttgattgcct tgctgttagt cgggttcttc
     2221 atcatatgcc tgatttggat gcggctctct cactgtttca tcaacatttg aaggaagatg
     2281 ggaaactcat cattgctgat tttaccaaga cagaagctaa tcatcatgga tttgatttag
     2341 ctgaactgga aaacaagcta attgagcatg gtttttcatc tgtgcatagt cagattctct
     2401 atagcgctga agacctgttt caaggaaatc actcagaatt ctttttaaca gtagcccaaa
     2461 aatcactcgc ctagtcaggg agtgattttt ctataaggat ggaaaaaaga agggaaattt
     2521 ggtaagatag taatatggat tttgaaaaaa ttgaacaagc ctatacctat ttactagaga
     2581 atgtccaagt catccaaagt gatttggcga ccaactttta tgacgccttg gtggagcaaa
     2641 acagcatcta tctggatggt gaaactgagc taaaccaggt caaagacaac aatcaggccc
     2701 ttaagcgttt agcactacgc aaagaagaat ggctcaagac ctaccagttt ctcttgatga
     2761 aggctgggga aacagaaccc ttgcaggcca atcaccagtt tacaccagat gccattgctt
     2821 tgcttttggt gtttattgtg gaagagttgt ttaaagagga ggaaattact atcctcgaaa
     2881 tgggttctgg gatgggaatt ctaggcgcta ctttcttgac ctcgcttgat aaaaaggtgg
     2941 attacttggg aatggaagtg gatgatttac tgattgatct ggcagctagc atggcagatg
     3001 taattggttt gcaggctggc tttgtccaag gagatgccgt tcgtccacaa atgctcaaag
     3061 aaagcgatgt ggtcatcagt gacttgcctg tcggctatta tcctgatgat gccgttgcgt
     3121 cgcgtcatca agttgcttct agccaagaac atacttacgc ccatcacttg ctcatggaac
     3181 aagggtttaa gtacctcaag tcagatggat atgctatttt tctagctccg agtgatttgt
     3241 tgaccagtcc tcaaagtgat ttgttgaaag tctggttgaa agaggaggca agtctggttg
     3301 ctatgattag tttacctgaa aatctctttg ctaatgccaa acaatctaag actattttta
     3361 tcttacagaa gaaaaatgaa atagcagtag agccttttgt ttatccactt gctagcttgc
     3421 aagatgcaag tgttttaatg aaatttaaag aaaattttca aaaatggact caaggtactg
     3481 aaatataaaa tagattttgt tataatagtt gaaaacgctt aaaaaggggt atcatgttat
     3541 gacaaaaaca attgcaatca atgcaggaag ttcaagtttg aaatggcaat tatacttaat
     3601 gccagaggaa aaagtgttag ccaaaggttt gattgaacgt atcggtttga aagattcaat
     3661 ttcaactgta aaatttgacg gccgttctga acaacaaatt ttggatatcg aaaatcatac
     3721 acaagccgtt aaaattttat tggatgactt gattcgtttc gatattatca aggcttatga
     3781 cgagattaca ggtgttggac atcgtgttgt tgctggtgga gaatatttca aagaatcaac
     3841 agttgttgag ggagatgttt tagaaaaagt tgaagagttg agtttgttgg ctcctctaca
     3901 caacccggcc aatgcagcag gtgttcgtgc cttcaaggaa ttgttgccag acattaccag
     3961 tgtagttgtt tttgatactt ccttccacac aagtatgcca gagaaagctt atcgctaccc
     4021 tctaccaaca aaatattaca cagaaaacaa ggttcgtaaa tacggtgctc atggtacaag
     4081 tcaccagttt gtagcaggag aagctgcaaa actcttggga cgtccattag aagacttgaa
     4141 gttaattacc tgtcatattg gtaacggagg ctcaattaca gctgtgaaag ccggcaaatc
     4201 tgtagacact tctatggggt tcactcctct tggtggtatt atgatgggaa cgcgtacagg
     4261 ggatattgat ccagctatca ttccttattt aatgcaatat acagaggatt ttaacacacc
     4321 agaagatatc agtcgtgttc ttaaccgtga atcaggtctt ttgggagttt ctgctaattc
     4381 tagcgatatg cgcgatatag aagcagctgt agcagaaggg aatcacgagg ctagcttggc
     4441 ttatgaaatg tatgttgacc gtatccaaaa acatatcggt cagtaccttg cagtgctaaa
     4501 tggagcagat gccattgttt tcacagcagg tgtcggtgaa aatgcagaga gtttccgtcg
     4561 tgatgtaatc tcagggattt cgtggtttgg ttgtgatgtt gatgatgaaa agaatgtctt
     4621 tggcgttaca ggagacatct caacagaggc agctaaaatc cgtgtcttgg ttattccaac
     4681 agatgaagaa ttagtcattg cccgtgacgt tgaacgcttg aaaaaataag tgaaactaaa
     4741 aaaatattca atacaaggag ttgggaaagt tatttttcca gcttcttttt ctgatgaaat
     4801 tgtccaaaac cttgctatga ttggcttttt tgaaaaatat ggtataatag tagtaattta
     4861 atagatggag ttgagttttg aagaaaaact ttcgtgtaaa aagagagaaa gattttaagg
     4921 cgattttcaa ggaggggaca agttttgcta atcgcaaatt tgtggtctac caattagaaa
     4981 accagaaaaa ccattttcga gtaggtctat cagttagcaa aaaactgggg aatgccgtca
     5041 ctagaaatca aattaagcga cggattcggc atattatcca gaatgcaaaa gggagtctgg
     5101 tagaagatgt cgactttgtt gtcattgctc gaaaaggagt cgaaaccttg ggatacgcag
     5161 agatggagaa aaatctactc catgtattaa aattatcaaa gatttaccgg gaaggaaatg
     5221 ggagtgaaaa agaaactaaa gttgactagt ttgctaggac tgtctctgtt aatcatgaca
     5281 gcctgtgcga ctaatggggt aactagcgat attacagccg aatcggctga tttttggagt
     5341 aaattggttt acttctttgc ggaaatcatt cgctttttat cgtttgatat tagtatcgga
     5401 gtggggatta ttctctttac ggtcttgatt cgtacagtcc tcttgccagt ctttcaggtg
     5461 caaatggtgg cttctaggaa aatgcaggaa gctcagccac gcattaaggc gcttcgagaa
     5521 caatatccag gtcgagatat ggaaagcaga accaaactag agcaggaaat gcgtaaagta
     5581 tttaaagaaa tgggtgtcag acagtcagac tctctttggc cgattttgat tcagatgccg
     5641 gttattttgg ccctgttcca agccctatca agagttgact ttttaaagac aggtcatttc
     5701 ttatggatta accttggtag tgtggataca acccttgttc ttccgatttt agcagcagta
     5761 ttcacctttt taagtacttg gttgtccaac aaagctttgt ctgagcgaaa tggcgctacg
     5821 actgcgatga tgtatgggat tccagtcttg atttttatct ttgcagttta tgcgccaggt
     5881 ggagtcgccc tatactggac agtgtctaat gcttatcaag tcttgcaaac ctatttcttg
     5941 aataatccat tcaagattat cgcagagcgc gaggccgtag tacaggcaca aaaagatttg
     6001 gaaaatagaa aaagaaaagc caagaaaaag gctcagaaaa cgaaataaat aaggaggaat
     6061 ctggtaatgg tagtatttac aggttcaact gttgaagaag caatccagaa aggattgaaa
     6121 gaattagata ttccaagaat gaaggctcat atcaaagtca tttctaggga gaaaaaaggc
     6181 tttcttggtc tatttggtaa aaaaccagcc caagtggata ttgaagcgat tagtgaaacg
     6241 actgttgtca aagcaaatca acaggtagta aaaggcgttc cgaaaaaaat caatgatttg
     6301 aacgagcctg tgaagacggt tagtgaagaa accgttgacc ttggtcatgt ggttaatgct
     6361 attaaaaaaa tagaggaaga aggtcaaggt atttctgatg aagtcaaggc tgaaatctta
     6421 aaacatgaaa gacatgccag cactatctta gaagaaactg gtcacattga gattttaaat
     6481 gaacttcaaa tcgaggaagc gatgagggaa gaagcaggcg ctgatgacct tgaaactgag
     6541 caagatcaaa ctgaaaatca agacttgaaa gagatgggct tgaaggtcga gcaaagttat
     6601 gatattgccc aggtggctac ggatgtgact gcctatgttc aagcgattgt ggatgacatg
     6661 gatgttgaag ctacactttc aaatgattat aaccgtcgta gcatcaatct acaaattgac
     6721 accaacgaac caggtcgtat tatcggctac catggtaaag tcttgaaggc cttgcaactg
     6781 ttggctcaaa attatcttta caaccgctat tccaaaacct tctacgttac aatcaatgtc
     6841 aatgattatg tcgaacaccg tgcagaagtc ttgcagacct atgcgcaaaa attggcgaat
     6901 cgtgttttgg aagaaggtcg cagtcataaa acagatccaa tgtcaaatag cgaacgcaag
     6961 attatccatc gtattatttc acgtatggat ggcgtgacta gttactctga aggtgatgag
     7021 ccaaatcgct atgttgttgt agatacagaa taagtaaaat cagggttgac ctgatttttt
     7081 gctagttaga ggaggttaaa ctgatgttga ataagataag agactatcta gacttttctg
     7141 gtttgcagta ccgtagtcct gataaagcgg gagcagagcg agagaagatg ctggcgttcc
     7201 gccacaaagg acaagaggcc cgaaaggctt ttacagaact ggccaaagcc tttcaagcaa
     7261 gccatccaga atggcaactc caacagacta gtcagtggat gaatcaggcc cagtgtttga
     7321 gaccacattt ttgggtttat ctacagagag acggacaagt gacagaacct atgatggcct
     7381 tacgtttgta tgggacatct actgactttg gaatttcttt ggaagtcagt ttcatcgaac
     7441 gtaagaagga tgagcaaaca ctgggcaagc agaccaaagt tttagacatt ccaaccgtta
     7501 aagggattta ttatctaacc tactctaatg gtcaaagtca acggtgggag gcgaatgaag
     7561 aaaagcgtcg tactttacgc gagaaggtga gaagtcaaga agttcgaaaa gttttagtga
     7621 aggtagatgt tcctatgaca gaaaattcgt ctgaagaaga aatcgtagaa ggcttattga
     7681 agtcttattc taaaattctt ccctattatc tagctacgag aaaataagat aatttgtaaa
     7741 acatcataaa tcatcataaa tcatacagtc caagagtgaa cagtccgctg tgtaattctt
     7801 ggtctttttg tttgcgcttt cgcattatat aataaactta caaaaacaat tcaaaaggag
     7861 aacaattatg gaagtcgttt caagtgttct aaattggttt tctagcaata ttttgcagaa
     7921 tcccgcattt ttcgtaggtt tattggtgtt gataggatat gcacttttga aaaaacctgc
     7981 ccatgacgtt ttttcagggt ttgttaaagc aacagtaggg tatatgttgc ttaacgtggg
     8041 tgctggtggt ttggttacaa cctttcgtcc aatcttagca gctcttaact acaaattcca
     8101 aattggtgca gcggttatcg acccttactt tggacttgct gcagcaaaca acaaaattgc
     8161 agcagagttt ccagattttg ttggaactgc aactacagct ctattgattg gttttggaat
     8221 aaatatcttg ctcgtagctc ttcgaaagat tacgaaggta agaaccctct ttattactgg
     8281 tcacatcatg gtacaacaag ctgcaacagt atctcttatg gttctattct tagtaccaca
     8341 attgcgcaat gcttacggta cagcagcgat tggtatcatc tgtggacttt actgggcagt
     8401 tagttcaaat atgactgttg aggcaactca acgcttgact ggtggtggcg gatttgcgat
     8461 tggtcaccaa cagcaatttg caatctggtt tgtagataaa gtagcaggac gctttggtaa
     8521 gaaagaagaa agtttagaca atcttaaatt acctaagttc ctctcaatct tccacgatac
     8581 agttgttgca tctgctacct tgatgctcgt attcttcgga gccattcttt taatcttggg
     8641 tccagacatt atgtctaata aagaagtcat cacttcagga actctattca atcctgctaa
     8701 acaagatttc tttatgtaca ttatccaaac agcctttacc ttctcagttt acttgttcgt
     8761 tttgatgcaa ggtgtccgaa tgttcgtatc tgagttgaca aacgccttcc aaggtatttc
     8821 aaacaaattg ttgccaggtt cattcccagc ggttgacgtt gcagcttctt atggatttgg
     8881 ttctccaaat gctgtcttgt caggatttac ctttggtttg attggtcaat tgattacaat
     8941 tgtcttgctc atcgtcttta aaaatccgat tcttattatt acaggatttg taccagtgtt
     9001 ctttgacaat gcagccattg cggtctacgc tgataaacgc ggcggatgga aagcggctgt
     9061 tatcctttcc tttatatcag gtgtccttca agttgctcta ggagctcttt gtgtggccct
     9121 tctcgatttg gcatcttatg gtggctacca tggaaatatc gactttgaat tcccatggct
     9181 tggatttgga tatatcttca aataccttgg tattgttggt tatgtacttg tgtgtctctt
     9241 cttgcttgtt attcctcaac ttcaatttgc caaagcaaaa gatagagaga aatattacaa
     9301 cggtgaagtt caagaagaag cttagtatct agaaaaggag aaataaaatg gttaaagtat
     9361 tagcagcgtg cggaaatgga atgggttcat caatggttat caagatgaag gttgaaaatg
     9421 ctctccgtaa gcttaatcaa acagatttta cagtcaattc atgcagtgtc ggtgaagcta
     9481 aaggtttagc agtaggatat gacatcgtaa tcgcttctct tcatttgatt caagaattgg
     9541 aagggcgaac taatgggaag ttaattgggc ttgataactt gatggatgat aaagaaatca
     9601 ccgaaaaact cagtcaagca ctacagtaaa aggttggagg ggctggacag aaactgagag
     9661 ttatcgtttc tgtccttctc cctctttaaa taaaggaggc agatatgaat ttaaaacaag
     9721 ctttaattga caatgactcg atccgactag gtttagaggc taacaattgg aaagaagcag
     9781 tcaaggtagc agtagatccc ttaattgaaa gtggggcaat tttgccagag tattacgatg
     9841 ctatcattga atcgactgaa gagtatgggc cttactatat cttgatgcca ggtatggcta
     9901 tgccccacgc tagacctgaa gctggtgtgc aaagtgatgc cttttcattg attaccttac
     9961 aaaatcctgt tgtattttca gatgggaaag aggtatctgt tttgttggca ctagcagcaa
    10021 caagttcaaa aattcacaca agtgtagcca ttccacaaat tattgccctg tttgaattag
    10081 aagattctat tgcacgttta caggcttgcc agactaaaga agatgtcttg gctatgattg
    10141 aagaatctaa ggatagccct tatctcgaag gattggattt ggaaagttag aaagaggaat
    10201 aaagaaatga caaaaagaat acctaattta caagttgcat tagaccattc agacttgcaa
    10261 ggagcgatta aagcagctgt ttctgttggt caggaagtag atattatcga agctggaact
    10321 gtttgcttgc ttcaagttgg aagtgaactg gctgaagtct tgcgtagcct tttcccagat
    10381 aagattattg tggcagacac aaaatgtgct gatgctggtg gaacagttgc taaaaataat
    10441 gcggttcgtg gagcagactg gatgacttgt atctgttgtg caaccatccc tactatggaa
    10501 gcagctctaa aggctatcaa gactgaacga ggagaacgag gcgaaatcca gatcgagctt
    10561 tatggcgatt ggacttttga acaagctcag ctttggctag atgcaggtat ttcacaagct
    10621 atttatcacc aatctcgtga tgctcttctt gctggtgaaa cttggggtga aaaagacctt
    10681 aataaggtta aaaaactcat tgacatgggc ttccgtgtat ctgtaacagg tggtctagat
    10741 gtagatactc tcaaactctt tgaaggtgtt gatgtcttta cctttatcgc aggtcgtgga
    10801 attacagagg ctgcggatcc agcaggagca gcgcgtgcct tcaaggatga aatcaaacga
    10861 atttgggggt aaatcatggt acgtccaatt ggaatttatg aaaaggcaac cccaacacac
    10921 tgtacttggc tagaacgttt aaattttgcc aaggagttag gctttgattt tgtcgagatg
    10981 tctattgacg aacgtgacga gcgtttagca agacttgact ggagtaagga agaacgcttg
    11041 gaagttgtca aagcaatcta tgaaactggt gttcgtattc cttctatctg tttttcaggc
    11101 catcgtcgct acccattggg ttcaaaagat ccagttctag aggaaaaatc tctagaactc
    11161 atgaaaaaat gtatcgaatt agctcaagac ttgggagttc gtacgattca attagctggt
    11221 tacgatgttt actatgagga aaagtcaccc cagacacgcc aacgttttat caaaaatttg
    11281 agaaaagcct gtgactgggc tgaagaagct caggtggtac ttgctattga aattatggat
    11341 gatcctttca tcaatagcat cgaaaaatat ttggctatag aaaaagagat tgactctccc
    11401 ttcctctttg tatatccaga tattggtaat gtgtctgcat ggcataatga tatctatagt
    11461 gagttttatc ttggtcatca tgccatcgca gctctccatc tcaaggatac ttatgcagtg
    11521 acagaaagtt caaagggcca gttccgagat gtaccttttg ggcaaggttg tgtcaaatgg
    11581 gaagaagctt tcgatatttt aaaggaaacc aattataatg gacctttcct aatcgaaatg
    11641 tggtctgaaa attgtgaaac agtagaagaa acacgcgcag ccattcaaga ggcgcaagct
    11701 tttctctatc cactcattaa gaaagcaggt ttgatgtaag atgaatcaag taatcaatgc
    11761 tatgcgtaaa cgagtttgtg atgccaatca atcattgcca aaacatggac ttgtcaaatt
    11821 tacctggggg aatgtatctg aagtcaatcg cgaactcggt gtcattgtta tcaaaccatc
    11881 aggcgtggat tatgacgaat tgacacctga aaacatggta gtgactgatc tagatggtaa
    11941 gatcctagaa ggggatttaa gaccatcttc cgacctccca actcatgtgc aattatataa
    12001 ggcttggtca gaaattggta gtgtggttca cacccattcg acagaagctg ttggttgggc
    12061 tcaggcaggt cgtgatattc ctttctacgg aacaacccat gcagattatt tctacggttc
    12121 aatcccttgc gcccgtagtt tgaccaagga cgaagtagaa gtggcctatg aaaaagatac
    12181 tggcctggtt atcgtagaag aatttgaaca tcgcggactt aacccggttg aagtaccagg
    12241 aattgttgta cgcaatcacg gtccattcac ctggggcaaa aatccagaga atgctgttta
    12301 tcactctgtc gtactagagg aagtatcaaa gatgaatcgc tttacagaac aaatcaatcc
    12361 aagagttgga cctgctcccc agtacatact agaaaaacac taccaacgta aacatggacc
    12421 aaatgcttat tatggtcaaa agtaagaacg atgaaggagg agaaaaagat aaatttagct
    12481 cctcttttta catttgattt ttattgagag taaagttgga gttgaagtaa ttttgaaaga
    12541 ttttttagaa atagcgcttg atatatatgg taaaataaaa agaattgctg tgatatcaat
    12601 agatttgggg gattttttaa tatggtactg gataaggcaa gttgtgattt gcttcaatat
    12661 ttgatggatc aagaaacgtc caaaacgatt atggcgattt cgaaagattt gaaagagtca
    12721 agaaggaaaa tttattatca cattgacaaa atcaatgctg ctctggttga cgaggcgctt
    12781 cacatcatta gtattccacg aattggtatt cacttaacgg aagagcagag agatgcttgt
    12841 tgtaaactat tatcggaagt agattcgtac gattatatca tgagtgcgca tgaacgtatg
    12901 atgataatgt tactatggat aggtatttct aaagaacgta ttacgattga aaaattgata
    12961 gagttaacag aggtatctag gaatactgtt ctcaatgatt tgaatagtat tcgttatcaa
    13021 ctaactttgg aacaatatca ggtgatcttg caagtgagca agtcacaggg ataccacctt
    13081 catgcccacc ctcttaataa aattcagtat cttcaatcgc ttctatatca tatttttatg
    13141 gaagaaaatg ccacttttgt atctatttta gaagataaga tgaaagagag gttagatgat
    13201 gagtgtttgc tttctgttga aatgaaccaa ttttttaagg aacaggttcc tttagttgaa
    13261 caagatttag ggaagaaaat aaaccatcat gaaataactt ttatgttgca ggttctacct
    13321 tatttgctgt taagctgtca taatgttgaa cagtatcaag aaagacatca ggatatagag
    13381 aaagaatttt ctttgataag aaaaagaata gagtatcagg tgtctaagaa attaggagaa
    13441 cggttgtttc aaaagtttga aatttctttg tcaggacttg aagtttctct tgtagctgtt
    13501 ctcctcctct cctatcgtaa ggatttggat attcatgcag aaagtgatga ttttcggcaa
    13561 ttaaaacttg ctttagaaga atttatctgg tattttgaat cacaaatccg aatggagatt
    13621 gagaacaagg atgatttgtt acgaaatttg atgatccact gtaaagcctt gttatttaga
    13681 aagacttacg gtattttttc taaaaatcct ctaacaaaac aaattcgatc caagtatgga
    13741 gaattatttt tagtcactag aaaatctgcg gaaattttag aaggagcatg gtttattcgg
    13801 ctaacagacg atgatattgc ctatttgacg attcatattg gaggattttt aaagtataca
    13861 ccatcgtctc aaaaaaatat gaaaaaagtt tatctcgttt gtgatgaagg tgttgcggtt
    13921 tcgagacttt tgctgaaaca atgcaaactt tattttccaa atgagcaaat tgacactgta
    13981 tttacaacag aacaatttaa gagtgtggaa gatattgcac aagttgatgt agtgattact
    14041 actaatgatg atttggatag cagatttccg attttaaggg ttaatcctat ccttgaagca
    14101 gaagatattt tgaaaatgct agactatctt aaacacaata tatttcgtaa taagagcaaa
    14161 agtttcagtg aaaatctttc tagtcttatt tcgtcttata ttgtagacag caagttggct
    14221 agtaagttcc aagaagaggt tcaaacactt ataaatcaag aaatagtagt tcaagctttt
    14281 ttggaagata tttgaaggac agtccaatga tgaacacaaa cctgtgtttt tcttggtctt
    14341 ttttagtgtt ttgaagggtg gtatactaat ctcaaagata acaattatat ccaaaggagg
    14401 caacatatgc caaacgtcaa agaaattaca agagagtcat ggattttagc cactttccca
    14461 gagtggggaa catggttgaa cgaagaaatc gaagaagaag tcgtacctga aggcaatttt
    14521 gccatgtggt ggctaggcaa ctgtggtact tggattaaga caccagctgg tgctaacgtt
    14581 gtcatggacc tttggtcaaa ccgtggaaaa tcaaccaaaa aagtgaaaga tatggttcgt
    14641 gggcaccaaa tggcaaatat ggcaggtgtt cgtaagttgc aaccaaactt gcgtgttcag
    14701 ccaatggtta tcgatccatt tgctatcaac gaactagact attacttagt ttcacacttc
    14761 cacagtgatc atatcgaccc atacacagct gcagcaattc tcaacaattc taagttagag
    14821 catgttaagt ttatcggtcc ttaccactgt ggacgaatct gggaaggatg gggtgttcca
    14881 aaagaacgta tcatcgttgt taaaccaggt gacactatcg aattaaaaga tatgaagatt
    14941 catgcagtag aatcatttga ccgtacttgc ttggtaactc tcccagtgaa cggtgctgat
    15001 gagacaggcg gtgaacttgc tggcttggct gttacagatg aagaaatggc tcaaaaggct
    15061 gttaactata tctttgaaac accaggtgga accatctatc atggtgcaga ttctcacttc
    15121 tcaaactatt ttgcaaaaca tggtaaagac tttaaaattg atgttgcttt gaataactat
    15181 ggtgaaaatc cggtaggtat ccaagacaaa atgacatcta tcgatcttct tcgtatggca
    15241 gaaaatctgc gtaccaaagt cattatccca gttcactatg atatctggtc taacttcatg
    15301 gcttctacta atgagattct agaactttgg aaaatgcgaa aagatcgctt gcaatacgat
    15361 ttccatccat ttatctggga agttggcggt aagtacactt atcctcaaga tcaacactta
    15421 gtagaatacc atcatccacg tggttttgat gattgttttg aacaagactc taacattcaa
    15481 tttaaagctt tgctataata taaaaatatt ctttggaggt tatccgtatg ttgcatgata
    15541 cggataattt tcatataata gtaaaataga atgtgtgatt caataatcac ctcaaataga
    15601 aaggaaattc tatgtcaaat ctatctgtta atgcaattcg ttttctaggt attgacgcca
    15661 ttaataaagc caactcaggt catccaggtg tggttatggg agcggctccg atggcttaca
    15721 gcctctttac aaaacaactt catatcaatc cagctcaacc aaattggatt aaccgcgacc
    15781 gctttattct ttcagcaggt catggttcaa tgctccttta tgctcttctt cacctttctg
    15841 gttttgaaga tgtcagcatg gatgagatta agagtttccg tcaatggggt tcaaaaacac
    15901 caggtcaccc agaatttggt catacggcag ggattgatgc tacgacaggt cctctagggc
    15961 aagggatttc aactgctact ggttttgccc aagcagaacg tttcttggca gccaaatata
    16021 accgtgaagg ctacaatatc tttgaccact atacttacgt tatctgtgga gacggagact
    16081 tgatggaagg tgtctcaagc gaggcagctt catacgcagg tttgcaaaaa cttgataagt
    16141 tggttgttct ttatgattca aatgatatca acttggatgg tgagacaaag gattccttta
    16201 cagaaagtgt tcgtgaccgt tacaatgcct acggttggca tactgccttg gttgaaaatg
    16261 gaacagactt ggaagccatc catgctgcta tcgaaacagc aaaagcttca ggcaagccat
    16321 ctttaattga agtgaagacg gttattggat atggttctcc aaacaaacaa ggaactaatg
    16381 ctgtacacgg cgcccctctt ggagcagatg aaactgcatc aactcgccaa gccctcggtt
    16441 gggactacga accatttgaa attccagaac aagtatatgc tgatttcaaa gaacatgttg
    16501 cagaccgtgg cgcatcagct tatcaagctt ggactaaatt agttgcagat tataaagaag
    16561 ctcatccaga actggctgca gaagtagaag ccatcatcga cggacgtgat ccagtcgaag
    16621 tgactccagc agacttccca gctttagaaa atggtttttc tcaagcaact cgtaattcaa
    16681 gtcaagatgc cttgaatgtt gtggcagcta aattaccaac cttcctaggt ggatcagctg
    16741 accttgctca ctcaaacatg acttatatca agacggatgg acttcaagac gataccaatc
    16801 gcttgaatcg taacattcag tttggtgttc gtgaatttgc aatgggaaca atcttgaacg
    16861 ggatggccct tcatggtgga cttcgtgtat acggtggaac tttcttcgtc ttctctgact
    16921 atgtgaaggc agctgtccgc ttgtcagcct tacaaggact tcctgtgact tatgtcttta
    16981 cccatgattc aatcgcagtt ggggaagatg gtccgactca cgaaccagtt gagcatttag
    17041 caggtcttcg tgctatgcca aatctaaatg ttttccgtcc agcagatgcg cgtgaaacgc
    17101 aagcagcttg gtaccttgca gtgacaagtg agaaaacacc aactgccctt gtcttgacac
    17161 gtcaaaactt gactgttgaa gatggaacag acttcgacaa ggttgctaaa ggtgcttatg
    17221 ttgtatatga aaatgcagcc gactttgata ccatcttgat tgcgacaggt tcagaggtta
    17281 atcttgctgt ctcagctgct aaagaattgg ctagtcaagg cgaaaaaatc cgcgtaatca
    17341 gcatgccatc tacagatgtc tttgataaac aaaatgcagc ttacaaggaa gagattcttc
    17401 caaatgcagt ccgccgtcgt gttgcagtcg aaatgggtgc aagtcaaaac tggtacaaat
    17461 atgttggtct cgatggtgcc gttctaggta ttgatacctt cggagcctct gccccagcac
    17521 caaaagtatt ggcagaatat ggctttactg tcgaaaatct tgtaaaagtt gttcgaaatt
    17581 tgaaataatc ctaaaaatca gggcgtaagc tctggttttt cttaccagaa aagtaaggta
    17641 caatcttgta aaagtagctg aaatttgata tagtagtcct atgtaaaaga caaaggagaa
    17701 aacaatgaat ccaaatatta cttttttaat catgcttgta ggtatgatgg ccttgatgtt
    17761 ctttatgcaa cgttctcaaa agaaacaagc tcaaaagcgt atggaaagct taaataaact
    17821 acaaaaaggc tatgaagtga ttacaatcgg tggactttat ggaacagtcg atgaagtaga
    17881 tacggagaag ggaacaatag ttcttgacgt agatggagtt tacttgactt ttgaactagc
    17941 tgctatcaag acagtattac cgctgaaaga aacagcttca ctagaaggcg caattgaaaa
    18001 ataagacggg atcacgaact cccgttttct ataaaagaaa ggaaatggga tgaaaaaatt
    18061 agtctttgtc tgtctgggaa atatttgccg tagccctatg gccgagtttg ttatgaaatc
    18121 aatgacagat aactacgaaa tccaaagtcg agcaacttcc tcttgggaac atggcaatcc
    18181 gattcataag gggactcagg gaatttttca agagtatgag attccttatg acaagaacaa
    18241 gacatcgctt cagattagta aggaagattt tgaagccttt gattatatta tcggaatgga
    18301 cgcttcaaat atttctgact tacgtcagat gtgtccagta gactgtcaag ataagattta
    18361 ctcattttca tctgaaagtg ttccagaccc ttggtataca ggagattttg aagaaaccta
    18421 tcgacgtgtt caagagggct gtcaagcttg gttagaacgt ttagaaaagg agagttaagg
    18481 tggaaaatct tacgaatttt tacgaaaagt atcgtgtcta tctgactcgt ccacgtttag
    18541 agcttttggc agtagttacc attgttttct gtgctgtact cgtctttttt ctaaatattc
    18601 caggaaaagg tgtcttaaaa ctcgataatg gaacgattgt ttatgatggc agtcttgtcc
    18661 gtggtaaaat gaatggccaa ggtaccatta ccttccaaaa tggagaccaa tatacaggtg
    18721 gcttcaacaa tggagccttc aacggaaaag gtacctttca atctaaagaa ggctggacct
    18781 acgaaggtga ttttgtaaat ggtcaggctg aaggaaaagg gaaactaaca acagaacaag
    18841 aagtcgttta tgaaggaact tttaaacaag gcgtttttca acaaaaataa agcctcctta
    18901 tcaaaggagg tattattaga atcacaaggt aagcgcttac ctgtaaatcc ctttctttcc
    18961 aaatccctct tccaagcaag tttgtgaaat aaaaaatatt tgaaataaat ttcacaaact
    19021 tcaaagataa aacttgataa gaaaagaaaa tgagaaaagt ttcacaagag tttaaaaatt
    19081 ctttgtgaaa tgtttatgaa actgtttaca aagttgaaat aatgcgttat aataaacttg
    19141 tgaaaaaatt aacaaaggat atcgttcctt gaaagctatg gaggaaaata tggctgataa
    19201 aaaaactgtg acaccagagg aaaagaaact cgttgctgaa aaacacgtag atgagttggt
    19261 tcaaaaagct ctagttgccc ttgaagaaat gcgtaaattg gatcaagaac aagttgacta
    19321 catcgttgcc aaagcatcag tagcagcttt ggatgcccac ggagaattgg ctttacatgc
    19381 ctttgaagaa acaggacgtg gtgtatttga agacaaagca actaagaact tgtttgcttg
    19441 tgaacacgta gtaaacaata tgcgccacac taagacagtt ggcgttatcg aagaagacga
    19501 tgtaacagga ttgactctta ttgctgaacc agttggtgtt gtttgtggta ttactccaac
    19561 aacaaaccca acatcaacag caatcttcaa atcattgatt tcattgaaga cacgtaaccc
    19621 aatcgtcttt gccttccatc catcagcaca agaatcatct gctcatgcag ctcgtatcgt
    19681 ccgcgatgca gctatcgcag ctggtgctcc tgaaaactgt gtgcaatgga ttactcaacc
    19741 atctatggaa gcaacaagtg cccttatgaa ccacgaaggt gttgcgacaa tccttgcaac
    19801 aggtggtaat gccatggtta aggcggctta ttcatgtggt aaaccagctc ttggggtagg
    19861 tgccggaaac gttccagctt atgttgaaaa atcagcaaac attcgtcaag cagcacacga
    19921 tatcgtcatg tctaaatcat ttgataacgg tatggtctgt gcatctgaac aagcagttat
    19981 cattgataaa gaaatttacg atgaatttgt agcagagttc aaatcttacc acacttactt
    20041 tgtaaacaaa aaagaaaaag ctcttcttga agagttctgc ttcggcgtca aagcaaacag
    20101 caaaaactgt gctggtgcaa aattgaacgc tgacatcgtt ggtaaaccag caacttggat
    20161 tgcagaacaa gcaggattta cagttccaga aggaacaaac attcttgctg cagaatgtaa
    20221 agaagttggc gaaaatgagc cattgactcg tgaaaaattg tcaccagtta ttgcagtttt
    20281 gaaatctgaa agccgtgaag atggtattac taaggctcgt caaatggttg aatttaacgg
    20341 tcttggacac tcagcagcta tccacacagc tgacgaagaa ttgactaaag aatttggtaa
    20401 agctgttaaa gctattcgtg ttatctgtaa ctcaccttct acttttggtg gtatcgggga
    20461 cgtttacaat gccttcttgc catcattgac acttggatgt ggttcttacg gacgcaactc
    20521 agttggggat aacgttagtg ccattaacct cttgaatatc aaaaaagtcg gaagacggag
    20581 aaataacatg caatggatga aacttccttc aaaaacatac tttgaacgtg attcaattca
    20641 ataccttcaa aaatgtcgtg acgttgaacg tgtcatgatc gttactgacc atgccatggt
    20701 agagcttggt ttccttgatc gtatcatcga acaactggac cttcgtcgca ataaggttgt
    20761 ttaccaaatc tttgcggatg tagaaccgga tccagatatc acaactgtaa accgtggtac
    20821 tgagattatg cgtgccttca aaccagatac catcatcgca ctcggtggtg ggtctccaat
    20881 ggatgctgcc aaagtaatgt ggctcttcta cgagcaacca gaagtggact tccgtgacct
    20941 tgtccaaaaa ttcatggata tccgtaaacg tgccttcaag ttcccattgc ttggtaagaa
    21001 gactaaattc atcgcgattc caactacatc tggtacagga tctgaagtaa caccatttgc
    21061 cgttatctct gataaagcaa acaaccgtaa atacccaatc gctgactact cattgacacc
    21121 aactgtggca atcgtagacc ctgctttggt attgacagtt ccaggatttg ttgctgctga
    21181 tactggtatg gacgtattga ctcacgcgac agaagcatac gtatcacaaa tggctagtga
    21241 ctacactgat ggtttagcac ttcaagccat taaattggtc tttgaaaatc tcgaaagctc
    21301 agttaagaat gcagacttcc actcacgtga gaaaatgcat aacgcttcaa caatcgctgg
    21361 tatggccttt gccaatgcct tcctaggtat ttctcactca atggcccata agattggtgc
    21421 gcaattccac acaatccacg gtcgtacaaa tgctatcttg cttccatacg ttatccgtta
    21481 caacggtaca cgtccagcta agacagcaac atggcctaag tacaactact accgtgcaga
    21541 tgaaaaatac caagatatcg cacgcatgct tggacttcca gcttctactc cagaagaagg
    21601 ggttgaatct tacgcaaaag ctgtctacga actcggtgaa cgtattggga tccaaatgaa
    21661 ttttagagac caaggaattg acgaaaaaga atggaaagaa cattctcgtg aattatcctt
    21721 cctggcttat gaagaccaat gttcaccagc taacccacgt cttccaatgg tagaccatat
    21781 gcaagaaatc atcgaagatg catactatgg ctacaaagaa agaccaggac gccgtaaata
    21841 attgtttatc agtctagaag caagacaaaa actcaatttg agggaaagat ccagtaattt
    21901 ttctatgata aaaggcatcc tatcaaggtt tttgaatacc tgataggatg cctttttatg
    21961 atgttgaggc ctttttgccc tttttgaaaa actagaatag aaacaaaata tatagcagat
    22021 tgaaactaga atagtacata tctgcttcta aaacattgtt agaattcgat ttgactgtcc
    22081 tgatcga
//
LOCUS       DAVKLW010000034        21808 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKLW010000034 DAVKLW010000000
VERSION     DAVKLW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.034"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(143..1366)
                     /locus_tag="VW324_001290"
     CDS             complement(143..1366)
                     /locus_tag="VW324_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9726928.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            1534..2856
                     /locus_tag="VW324_001291"
     CDS             1534..2856
                     /locus_tag="VW324_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9726929.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDSVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            2858..4744
                     /locus_tag="VW324_001292"
     CDS             2858..4744
                     /locus_tag="VW324_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9726930.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEEKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRTLNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            4912..5256
                     /locus_tag="VW324_001293"
     CDS             4912..5256
                     /locus_tag="VW324_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9726931.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            5266..6678
                     /gene="lacG"
                     /locus_tag="VW324_001294"
     CDS             5266..6678
                     /gene="lacG"
                     /locus_tag="VW324_001294"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9726932.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            6748..8427
                     /locus_tag="VW324_001295"
     CDS             6748..8427
                     /locus_tag="VW324_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9726933.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(8553..9992)
                     /locus_tag="VW324_001296"
     CDS             complement(8553..9992)
                     /locus_tag="VW324_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9726934.1"
                     /translation="MNKSMIHYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(9996..11345)
                     /gene="trkA"
                     /locus_tag="VW324_001297"
     CDS             complement(9996..11345)
                     /gene="trkA"
                     /locus_tag="VW324_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9726935.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKEVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11533..12381
                     /locus_tag="VW324_001298"
     CDS             11533..12381
                     /locus_tag="VW324_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9726936.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            12396..12944
                     /locus_tag="VW324_001299"
     CDS             12396..12944
                     /locus_tag="VW324_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9726937.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            12951..13199
                     /locus_tag="VW324_001300"
     CDS             12951..13199
                     /locus_tag="VW324_001300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726938.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVR"
     gene            13310..14992
                     /locus_tag="VW324_001301"
     CDS             13310..14992
                     /locus_tag="VW324_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726939.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKKEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            14977..15807
                     /locus_tag="VW324_001302"
     CDS             14977..15807
                     /locus_tag="VW324_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9726940.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGAYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            16000..16503
                     /locus_tag="VW324_001303"
     CDS             16000..16503
                     /locus_tag="VW324_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9726941.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      16557..16791
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            16956..17519
                     /locus_tag="VW324_001304"
     CDS             16956..17519
                     /locus_tag="VW324_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9726942.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            17533..18159
                     /locus_tag="VW324_001305"
     CDS             17533..18159
                     /locus_tag="VW324_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9726943.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            18186..18602
                     /locus_tag="VW324_001306"
     CDS             18186..18602
                     /locus_tag="VW324_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726944.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            18500..18811
                     /locus_tag="VW324_001307"
                     /pseudo
     CDS             18500..18811
                     /locus_tag="VW324_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            18939..19526
                     /locus_tag="VW324_001308"
     CDS             18939..19526
                     /locus_tag="VW324_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9726945.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            19916..21523
                     /locus_tag="VW324_001309"
     CDS             19916..21523
                     /locus_tag="VW324_001309"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9726946.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6501 a         3710 c         4797 g         6800 t
ORIGIN      
        1 aaagttattt taatataaat cactcaacat agcgagaagg cggtttccta tatggaagtc
       61 gtcttgtttt catattgtct aaatacgaga tatctactac aggttatagt tgatttaaac
      121 aggtctatag atttctaata tattaaggtg aaataatatc ttgtaataca ttgctatgtt
      181 ctatgaaaaa agcgacgata gctaatgcac aagctcctat acttccacga tgtttgttgt
      241 aaggtaaaat ttcaatttct atattacgag taaagggaac gaagtggagc tggtcttgga
      301 ttttatccct gacttcatga gtgaaaggtt gataattaag caattgactg ttgatataga
      361 ttttatcagc aggattgatg aggaggagat ttgcaataga agtggctaat aaattgactc
      421 ctttatcaat ttgttgacgt aaagcggagt cgcctaaatt ataagccgtt aaaagggtgt
      481 ctaaatgaat gtctttttca gtctttacaa ggctttttag tacagttagt tgggaatttt
      541 taaataataa ttgggcgtgt ttgattaacc aagcatccga aatatatgtt tgtaaacaac
      601 ctttttttcc gcattcacaa tattgcccat tcaattcgac aatggtgtgt ccaatttctc
      661 cgatataagg atttttagag gctcctattt tttcttttgt gaaaaaggaa gtgtaaatac
      721 ctaatccagc gtgtaggaaa ataaagttat cgggggtatt gtgtggatta aaaaggtatt
      781 gtcctatagc catacaatcg atattatttt ttacaataaa aggaaaattg aatcttttaa
      841 ttacatttaa taaattaaaa gattcccata tggggttgtt tgtaatgata cttccggagt
      901 ctttgttata atgtccaggt attgataagc caaatccaag taattttgtt tccaaaggga
      961 cgtactctat agctgttttt atcatattgg taatggtgga attgatttct gaaataggat
     1021 aatcttcatt caaaatttct gtctcgaaaa gagttttccc ttgtaagtca catagactga
     1081 gaaagatacc taggacgttt atttcaatac ctccaacaac atacggaata ttatctgtga
     1141 tagaaatagt ttttctacgt cttccagatc cggaaaactc gctaaattca tcaccagttt
     1201 cgtatatgag ggactgttcg cttagctctt ttatgatgtt agtcgttgtt gctggagtaa
     1261 gtcctgtttc attaacaata tctgtacggg atgttgctgg atttcgatat agaaactcca
     1321 gtatagtggc tctattttgt aaaaaggctg attgttttgt aatcatttgt ttcctccttt
     1381 ggtttatttt atattaaaca taagaaaaaa tcaattaaaa ttattattct atacaaagaa
     1441 aaaaatattg taaaaacaac aaaaaatata ttgacaacgc tttctgttag tggtatattt
     1501 aatttataaa aaataaaaaa aggagttccg gtgatgaaca atattttagc gtttttagaa
     1561 acaaaagtcg ctccgtttgg tgaaaaagtt ggcaaccaac ggcatttgaa agctattcgt
     1621 gaaggattta tgatggcaat gcctttgatt ttagtcggat ctttatttct tattctaatc
     1681 agttggcctc aagaggattt tacaaattgg ctgaatagtg ttggattgct aagtatcttg
     1741 acaactatga atcagtcaac agtagcgatt atctccttgg tcgcttgttt cggtattgcc
     1801 tacaggttgt cggaaggata tggtacagat ggtccgtcgg cagggatcat agccttatcc
     1861 agttttgtat tgatggcacc tcgtttttcg agtatggttt atgataaaaa tggggagcag
     1921 gtcaagcagt tatttggcgg cgcaatacca ttttctagcc tgaatgcatc ttctttgttt
     1981 atggcgatta ctattggatt ggttacagca gagatttatc gtatgtttat ccagcgcgga
     2041 attacgataa aaatgccaag tggtgtccca gatgtggtaa gtaaatcatt ttcagctctt
     2101 ttacctggct ttactacttt tgttttgtgg gctttggtct taaaaggtct tgaagcggca
     2161 ggagttgcag gaggtctcaa cggactccta ggtgcaattg ttggaacacc gcttaagtta
     2221 attgcaggaa cgcttccagg tatgattcta tgtgttattg taaactcatt cttttggttc
     2281 tgtggagtta atgggggaca agttttaaat gcttttgtag actcagtttg gttacaattt
     2341 actacagaaa accaagaagc tgtggctgca ggacaaacac tccaacacat tattacatta
     2401 ccgtttaaag atttatttgt atttattggt ggcggtggag cgactattgg tcttgcgatt
     2461 tgtctcttcc tatttagtaa gagtcgtgcg aataaaacat taggtaagct agctattata
     2521 ccgtctattt ttaatatcaa tacagctatt ctatttacgt ttccaacagt tttaaatccg
     2581 attatgctga ttccgtttat tgctactcct acaatcaatg ccttgattac ctatgtatca
     2641 atggctgtag gattagtacc ctatacaaca ggtgtaatcc ttccgtggac aatgccaccg
     2701 attataggag gcttccttgc aacaggggct agttggcgag gagctctatt acaagttgtt
     2761 ttgattttgg tttctgtagc aatttattat ccattcttca aaattgcaga taaacgcaat
     2821 cttgaaaaag aaaaagctac tgttggaggg aaataagatg gttatcagag tatttgatca
     2881 acagaaaaat acttattcta gctttgcctt agaggaatta agttactata tgaatcgggt
     2941 ctttaagact aacatagagc ttgtcgagga gaaggaagcg gatatttttg taggattagt
     3001 caataaagag gacagaaaag accatgttct tatctcatta gacaagggta aggggagaat
     3061 tgagtctaat acaattgtag gtttacttat tggaatttac cgaatgtttc atgaatttgg
     3121 ggttgtgtat actagaccag ggcgcagaca tgactttgtt ccagagttac gatttgaaga
     3181 ttttttagat aaacagctat ctatagatga aacagccagt tactatcata ggggagtatg
     3241 tatagaggga gcggattcat ttgaaaatat actagatttc attgattggc tacctaagat
     3301 tgggatgaac agttttttca tccagtttga aaatccttac tcttttttga aacgttggta
     3361 tgaacatgag tttaatccat acctaaataa agaaaaattc tcaaatgaat tagtacaaga
     3421 attgagtgat aggttggata aagaattgca aaaaagaggt cttattcatc atcgtgttgg
     3481 tcatggatgg acaggtgagg ttttaggtta ctcttcaaaa tttggctggg aatcaggtct
     3541 tagtatttca gaggaggaga aaccgtatgt cgctgaaata aacgggaaac gagaattatt
     3601 taatacggct ccgattttaa ccagtctgga tttttcaaat ccagatgtgg cggataagat
     3661 ggtagaaatt atcaaggatt atgccaagaa aagacctgat gttaactact tacatgtatg
     3721 gttgtcggat gctcgtaata atatttgtga atgcgaaaac tgtagacaag aattggtttc
     3781 ggatcagtat attcgtactc tcaatcaatt ggatagggct ttaacgagtg agggattaga
     3841 tacaaagatt tgttttctgc tttaccatga gttgctatgg gcgcctcaga aagaaaaatt
     3901 agataatcct gaacgcttta ccatgatgtt tgcaccgatt acaagaacat ttgaaatgag
     3961 ttatgcagat gtagattttg acaattccat acctacgcct aaaccttatc tgcgtaataa
     4021 aattatactt ccgaattctc ttgaggaaaa tttatcttat ctttttgagt ggcaaaaaac
     4081 atttaaagga gatagtttcg tatatgacta tcctttaggg cgtgctcatt atggcgattt
     4141 aggctatatg aaaattagtc aaaccattta cagagatgta tcttatcttt ctaacctaca
     4201 tttgaacggt tacatttcgt gtcaagaatt acgtgccgga ttccctcata attttcctaa
     4261 ttatgttatg ggggaaatgc tctggaagaa gacaagaagt tatgaagaat tgattgaaga
     4321 atacttttct gctttgtatg gggaaaattg gcagtctgtt gttgaatatt tagaaaaatt
     4381 atccagttat tcctcatgtg attattttaa tgcaattggc agtcgtcaaa atgatgtttt
     4441 agcgaatcat tattatatag cttacaatct agctgataat tttttgccaa ttattgagga
     4501 aaatatttct aagttattaa atagtcaaaa ggatgaatgg aaacagctca gttatcatcg
     4561 tgaatatgtt gttaagatgg cgaaggcttt atatcttcaa gcaaccggaa aaacaaggca
     4621 agctcaagat gaatggagaa atgtgttgaa ttatatccgt gggcacgaac tgctattcca
     4681 atctaatttg gatgtttatc gtgtaattga agtagcaaaa aattacgctg gtttccactt
     4741 ataaatcata agtatagaaa atgaaccaag gtattcagag aagattgatc ctaaatatta
     4801 tgaaatttaa ggatttttaa gatatttagg gtcgactttc tatttatatc gtagcgaagt
     4861 cattttaata atgatgtgta aaagatggat caagattgag gaggaagaaa gatgaaatca
     4921 aaagaagaaa taaatatgct tggttttaca attgtcgctt acgcaggaga tgcaaggtca
     4981 gatttgatgg atgctttggc gtttgcgaga gatggatatt ttgaacaggc aagagaattg
     5041 gttgagtctg caaacgactc aatagtgtct gcccatcgag aacagactaa tttattagcg
     5101 gaggaggcat ctggagataa ttttgaagtg agttttatca tgattcatgg tcaagatact
     5161 ttgatgacaa cgatgctatt gtatgatcag gtcaagtttt ttattgatga atatgaacga
     5221 attcgaaaga ttgaagaaca tattggtttg caatgaggat tagtcatgga aaatttacag
     5281 gttaaagcct taccgaagga gtttttatta ggagctgcta ccgctgctta tcaagtagag
     5341 ggtgcaacta gggtagatgg caaaggaaca aatatgtggg atgtttattt gcaagaaaac
     5401 agtccgttct taccagatcc agctagtgat ttttattatc gttacgaaga ggatatagct
     5461 ttggcggcag aacatggttt gcaggctttg cgtttatcta tttcttgggt tcgtatattt
     5521 cctgatatag atggggagcc taatatatta gctgttcgtt attaccatag agtctttcag
     5581 tcttgcttaa aacataatgt gattccgttt gtttctttac atcattttga ttcgcctcag
     5641 aaaatgttag aaacagggga ttggttgaat agagagaata ttgatcgttt catacgatat
     5701 gctcgctttt gtttccaaga atttacagaa gtcaagcatt ggtttacaat caatgaactg
     5761 atgtctcttg ctgcaggtca atatatagga ggtcagtttc ctccaaatca tcattttcaa
     5821 ttatctgaag caattcaagc gaatcataat atgttgttgg cgcatgctct tgcagtcctc
     5881 gaatttcatc aattagggat tgagggaaag ataggttgta ttcatgcttt aaagccaggc
     5941 tatcctattg atgggcaaaa agaaaatatt ttagcagcta aacggtatga tgtttataat
     6001 aataaatttc tattagatgg aacttttttg ggctactaca gtgatgacac gctttttcac
     6061 ttgaatcaaa tattggaagc taataattct agctttatta ttgaagatgg tgatttagaa
     6121 attatgaaaa gagctgcacc tcttaatacg atgtttggga tgaattatta tcgttcagaa
     6181 tttattcgtg aatacaaagg tgaaaataga caagaattta attcaacagg aataaaagga
     6241 cagtcttctt ttaaattaaa tgctctaggt gaatttgtaa aaaagcctgg tattccgaca
     6301 acagattggg attggaatat ttatcctcaa gggttatttg atatgttgct tcgtatcaaa
     6361 gaagaatatc ctcaacatcc ggtcatttat ttaactgaaa atggtacagc ccttaaagag
     6421 gttaagccag agggcgagaa tgatattatt gatgacagta aaagaatccg ttatattgag
     6481 caacatttac acaaagtttt agaggctcga gataggggag tcaatattca aggctatttt
     6541 atatggtctt tgcaagatca attttcttgg gcgaatggct acaataagcg atatggtctt
     6601 ttctttgttg attatgaaac acagaagaga tatattaaga aaagtgctct ttgggtaaaa
     6661 ggattaaaac ggaattaagg ttagcgattt gactgatgtt taatatattt taactatgag
     6721 cttaaatttt ttacaggagg agtttttatg gataagttag tcgctgccat tgaaaagcaa
     6781 caagggaaat ttgaaaaaat ttctactaat aattatatga tggctattaa agatggattt
     6841 attgctacta tgcctttaat tatgttttca agctttttga tgattattat tatgattcct
     6901 aaaaatttcg gagtagagtt accgagtcca gctattgcct ggatgaggaa agtgtatatg
     6961 ctaaccatgg gagttttggg tgttattgtt tcagggacag ttgcaaagtc attggctgga
     7021 aatgttaata gaaaaatgcc tcctggaaag gtaataaatg atacttctgc aatgttggcg
     7081 gccatatgtg gctatcttgt attaactgta acgcttgtag ttgatgagaa aacgggatct
     7141 acaagtttgt cgacaaacta tttaggatct caaggattga taacttcgtt tgtcagtacc
     7201 tttattactg taaatgttta ccgattctgt attaagcgag acattactat tcatttacct
     7261 aaggaagttc ctggggctat atcacaagct tttagagata tttttccttt ttcttttgtt
     7321 ttacttatta gtggtttgtt agatattgta tctcggttta gtttagatgt tccttttgcc
     7381 caagtatttc aacaactatt gactcctatt tttaaggggg cagaatcata tcctgctatg
     7441 atgttgattt ggtttatgtg cgctttgctt tggtttgttg gaattcatgg accatctatt
     7501 gtcttacctg ctgttacagc tttacaactg agcaatatgg aagagaatgc tcaacttctt
     7561 gcaaatgggc agtttcctta tcattcttta acgcctaatt tcggaaatta tatcgctgct
     7621 atcggaggaa cgggggctac ctttgttgta ccatttattt tgattttctt tatgcggtct
     7681 aaacaattaa aatcggtagg taaagctaca attactcctg ttttatttgc ggtgaatgaa
     7741 cctcttctat ttggtatgcc agttattttg aatccctatc tttttgtacc ttttttgatg
     7801 actccaccag tgaatgtatt tctaggaaag gtctttattg atttctttgg aatgaatgga
     7861 ttttatatcc agttaccttg ggcctttcct ggtcccttgg gattgttaat tggaacgaat
     7921 tttcaatcta tctcctttgt atttttatct ttgattttag ttgtcgacat attgatttat
     7981 ttgccattct gtagagcgta tgatagacag ttactgatga aagaagatgt tgcaagctca
     8041 aatgatatta ttttagagga ggagacaagt gaaataatgc ctggtgagtt agatgaaata
     8101 aaaagtaagg agttgaaagt actggttctt tgtgcagggt ctggaacaag tgcgcaatta
     8161 gccaatgcaa ttaacgaggg cgctcaatta gcagaagtta gagtgattgc gaattcagga
     8221 gcgtacggag ctcattatga tattatgggt gtttatgatt taattattct ggctccacaa
     8281 gttcggagtt attatagaga gatgaaggtg gatgcagaaa gattaggtat tcagatagtt
     8341 gctaccagag gaatggaata tattcattta acaaagagtc caagtaaatc cttacaattt
     8401 gtattggagc attaccaagc tgtgtagtaa gtttttccat cttttatttg agtaaggatt
     8461 ttgcttacag ataggcttgg atttaaaaac gttccccctt ttttaatata agaatccctc
     8521 tttcacaatt gtaaaaagag ggattttgta ttttatctct tagaccaagt tctcttcata
     8581 aagagaagga ggattgggta aatctccaag cgccctgcaa tcattgcaaa ggataggaga
     8641 atttttgaga tgggactaaa gattgagaaa ctagaagtgg ttcctagaat aggcccgata
     8701 ttattgaaac agctaaagac agcgctggtc acgaccagaa aatcattgct atctaggctg
     8761 acaataaaga taagcgctag caaaatcata gcatagatga caaagtactt gagaatctta
     8821 tgctgggtat ctttatcaat caccgtttga ttcacatgga gggtcaaaac acggtggggc
     8881 gataggattg acaaaatttg gtttttggca atttttgaaa ggatgaggcc tcgaataatc
     8941 ttgagtccac ctgcagttga tccagcagag ccaccgattg ccatgaggaa aaggaggata
     9001 aactgggaga agaggggcca gttggtaata tctccatatc caaaaccagt tgttgtaatg
     9061 atgttggaaa cctggaagaa ggtcatttca aagctctttg aaaaccctgg gtagaggtag
     9121 agggtgttga ggctaatcaa gcctgtagaa accagtacaa tgaccaagta agcccgaagt
     9181 tcttcgtcac caaagaaggc cttgatgcga cggagcatga ggtagtagta gaggttgaaa
     9241 tttaccccaa aaaccagaac tccgatactg accagatagg taatcagtga gctgccatag
     9301 tgggcaattc cgtcgttata gacggtaaag cctccagttc ccgctgtccc catagcaatg
     9361 acaaaactat cgtagagagg cataccagct agataataga tgatgacaaa gagggagaag
     9421 agagctagat aaaggagata gagaatctgg gcagtgtttt ttagtttgga tacaaccttg
     9481 ccaaaaacag gacctggaac ctcagccttc atcacctcta ggtggctatt tttggcattg
     9541 tccataatag caagtgcaaa aacaagcact cccatccctc caatcaagtg ggtaaaactt
     9601 cgccagaaga ggagggaacg gctgagaacc gaaacgtcgt tcaaaatact tgctccagta
     9661 gttgtaaacc cagaactaat ttcaaaaaag gcatcaataa ggctggggat ttgcccagaa
     9721 aagacaaagg ggagaccacc aaagaaagac caaaggatcc aacagagggc aacgatcaag
     9781 actccctcct tggcataaat ccgttgattt tttggcttct gtaaactccc tgaaccgcct
     9841 aacaatacga gaatccctat ggttgaaaag agggctgtaa agacttggct cgattcacgg
     9901 taatagacag cgatagccac aggaaccaag aggagaacag cttcaatcaa aagtaacttt
     9961 gaaaggaggt aatgaatcat acttttattc atttcttacc tcgcaatcaa gtcataaatc
    10021 ttggtgatgt ttggcaacaa ggttgttact aggagcttgt ctccaacttc caacatatcc
    10081 tccccagttg ggaaaatagt cttgcccttt cgaataatgg ctgcaataag aacctctttt
    10141 ttcaatttca gttgagaaag aggtttggca gtcattttat tggcttcctt gatatggaat
    10201 tgcagggttt cgatttggcc attggctaga tggtgcatgg cttgaaggtc tgaatactgg
    10261 gcattaactc gaccacgaat aaagtgcata atcgtatcta cagcgatgct tttaggtgtg
    10321 atgatacttg aaaaatcagg cgcattgata atctcgagga gactggtacg attgacctta
    10381 gtaatatttt tctgtacacc taccctgtca aggaacatag atgtaatcag attttcctca
    10441 tcgactcctg ttagagtcgc aacggcatca tagtgttgag cactttcttc cagcaggata
    10501 tcttttgcgg ttccatctcc ttgaacgatg tagagatttg ggaatttctc gctaaagaag
    10561 ctggcgattt caggattgat ttcaatgact tttgtatcga tacgactatc tttgagaata
    10621 ccaagtagat aataggcaat tctacctgct ccaacgatga gaaggctctt cacggcgcgt
    10681 gatttaaaat aattatggaa gagtatcata tcgacacggt taccagtgac aaagattcta
    10741 tctttatcct gtacagtcat gtcaccgctt ggaatgataa tttgatgatc cctctctatc
    10801 gcacagacaa tgacattacc aaatttttta cgaaaatcag aaatgggcat ttggcaaaga
    10861 ccgctggtgg acttgacgac aaattccatg aggctaacgc gtccaccagc aaagcgttcg
    10921 acagacaggg cgttggggaa gtcaatgata ttcgcgatag cgcgggcagc caagagctca
    10981 ggattaacga taagagaaaa accgagaata ttcttttcct tgaaataaga gttagaatat
    11041 tcagggttcc gcacccgaac gatagtttct ttagctccca ttttcttggc tagaactgct
    11101 gcaatcatgt tgacttcatc gtgctcagtc agggcgataa agatatcaca atcttggacg
    11161 ctggcttgct caagaatggc aaaatcggcc ccgttaccaa ggataccaat gatatcaaag
    11221 cgactgacaa tatgattgag aacagcttcg tcttgctcaa tcagcaaaac atcatgcttt
    11281 tctgcaacca aggagcgaca gagggcaaaa ccaacttttc cccctccgac aaggataatt
    11341 ttcataataa aacctacttt ttcatgatgt aactatcata ccctttttca agaaaaaatg
    11401 cacctactag ctaataacaa gagcttttag tgaaaattcg ctataaggta aaactatacc
    11461 ctaaccaatt gaaatagcta ttagcgactt tctctgaaat atggtatgat aaaggatata
    11521 caaggagata aaatgaacaa taatttactg gtattacaat cagactttgg tctggttgat
    11581 ggtgcggtgt cggctatgat tggagtggct ttagaagagt ctccaacctt aaaaatccat
    11641 cacttgacgc acgatatcac gccttataat atttttgagg ggagctatcg tctctttcag
    11701 acggtggatt actggcctga gggaacgacg tttgtatcgg ttgtcgatcc aggtgtcggt
    11761 tcgaaacgta agagtgtagt tgccaagact gcaaaaaatc aatacattgt cacgccagat
    11821 aatgggacgc tttcctttat caagaaacac gttggcattg tagccattcg tgagatttct
    11881 gaggtggcca ataggcgtca aaacacagag cattcttata ccttccacgg tcgtgatgtc
    11941 tatgcctata ctggtgctaa actggccagt ggtcacatta cttttgagga agtagggcca
    12001 gagctcagtg tggaacagat tgtagagctt ccagtcgtag cgaccatcat agaagatcat
    12061 ctggtgaagg gagccattga tattctggat gtgcgtttcg gttcgctttg gacctctatc
    12121 acacgggaag aattttacaa gctggaacca gaatttggtg atcgttttga agtgaccatc
    12181 tatcatgctg atatgctggt ttatcaaaat caggttgtct atggcaaatc atttgcagat
    12241 gtgagaattg ggcaacccat cctttacatc aactctctct atcgtttagg tttggctatc
    12301 aaccaaggtt cctttgccaa ggcctataat gtaggtgtcg gttcatcttg gaccattgaa
    12361 ataaagaaaa tagaaggata aaataggaga atagtatgga aattaaattt acaattaaac
    12421 aagttgttgc tgtcggaatt ggcgctgccc tctttgtcgt catcgggatg atcaacattc
    12481 caacccctgt tccaaataca agcatccagc ttcagtatgc ggtacaagcg ctactttcta
    12541 ttatttttgg accgattatc ggtttgcttg tcgggttgat tggtcatgca atcaaggact
    12601 ctcttgctgg ttatggtcta tggtggactt ggattatcgc tagcggactc tttggtttag
    12661 ttgtgggact ttttagaaag tacgttcgcg tgatcaatgg tgtttttgac tggaaagata
    12721 ttcttatttt taacctcatt caactacttg caaatgccct tgtttggggt gtcttggcac
    12781 cacttggaga tgttgtgatt tatcaagaag cggcagaaaa agtatttgca caagggattg
    12841 ttgcgggaat tgccaatggt gtatctgtag ctattgcagg aactcttctc ttacttgcct
    12901 atgcaggaac ccaaactcgt gcaggaagtt tgaaaaagga ctaatactca atgaaaatca
    12961 aagagcaaac taggaagtta gccgcaggtt gctcaaaaca gtgttttgaa attgtggata
    13021 gaactgacga agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca
    13081 gtaaccatac ctacggcaag gcgaagctga cgtggtttga agagattttc gaagagtata
    13141 aaatgatggg gaaggctggg caactagtct ttttcgatgt ttatagatta gttaggtaag
    13201 taagctagga taggaatctt tttggcgcta agattttaag aggagagact gcaagaaggg
    13261 cttgatttat gataaaatag aagtctaaga agcgaatgaa gagagtaaga tgaaagaagc
    13321 tataattgag tggaaggatt tctctttccg gtatgaaaca caacaagaac cgaccttgca
    13381 agggatagac ttgaccattt acaagggaga gaaagtctta attgttggac catctggatc
    13441 aggtaaatct accttgggtc agtgtttgaa tgggattatt cccaatattt acaagggtca
    13501 gacatatgga gaatttttga taaagggtca aacagccttt gatatgagca tctatgataa
    13561 gtctcatctg gttagcacag ttttgcagga tacagatggg cagtttattg gcttgtctgt
    13621 ggcagaagat ttggcgtttg ctctggaaaa tgatgtgaca gccctagatg agatgaaagg
    13681 tcgtgtttat aaatgggctg aaaagctgga ccttcttcct ttactggatc agcgccctca
    13741 ggatttgtca ggtggacaaa agcagcgagt cagtctggct ggtgtcttga ttgatgaaag
    13801 tccgattctc ttgtttgatg agccactcgc caatctagat cccaagtcag gtcaggatat
    13861 tatcgaattg attgaccaga ttcataagga agaggggacg acgactctta ttatcgagca
    13921 ccgtttggag gatgttctgc atcgccctgt ggatcggatt gtcttgataa acgatggtcg
    13981 tatccttttt aatgggagcc ctgaccagtt gcttgcaact gatttattga ctcaaaatgg
    14041 aattcgagaa cccctttatc taacgactct ccgtcaatta ggtgtggact tagtcaagaa
    14101 agaacagtta gcgaatctgg ataacatgtc tatctcaaaa ggtcaggttc agctgcagaa
    14161 tgaactggca aaagaaactc cagaattgca gtcactcttt aaattagagg acgtgtcttt
    14221 ctcttatgat gatagaccga ttttaaaatc cctacattta gatattaaaa agggtgaaaa
    14281 gattgctatt gtcggaaaaa atggagcagg gaaatcaact ctagccaagg ctataagtag
    14341 ctttattcag acggaaggac gctatctttg ggaaaaacag gatataaaag gcgattctgt
    14401 tgcagagcgg gcggaacgag taggctatgt gctacaaaat cctaatcaaa tgatttcaac
    14461 caatatgatt tttgatgagg tggctctagg gctccgtttg cgaggtgtgg atgagaagga
    14521 aattgaaacg agagtctatg aaaccttgaa aatctgtgga ctttatgaat tccgtaattg
    14581 gcctatttct gccctgtcat ttggtcagaa aaaacgtgtc accattgctt caattttggt
    14641 cttaggagct gaaattattc tcctagatga accgactgca ggtcaagatc agaagaacta
    14701 tactgagatt atggaatttc tcgaagagtt acatcaaaaa gggcatacca ttgtcatgat
    14761 tacccatgat atgcaattga tgctggatta ttcagaccgg gtccttgtca tggtggatgg
    14821 agaattgatt gccgatactg ttccagccag tctgttgagc gatcctgagc tgttagtaaa
    14881 agccaatcta aaagaaacct ccatctttaa cttggctaag aaactagatg tggatccact
    14941 ggatttaacg gcattttaca aagaaaggag agagggatgc aagctaaatt aatcggttac
    15001 cagcatagag atactgtgat tcatcgcttg tcgggagctg ggaaacttct ctttttcatt
    15061 ctggtatcat tggcggccat gattagctat gataccagaa tgctcttgct gattgccatc
    15121 ttttcagtct ttctcctcta tttatcagaa attcgcttta aagatgtttc ctttgtagcc
    15181 gtttttgcga cggtatttgc cgttttaaac gttttgatgg tctatctctt ttctcccgag
    15241 tatggggttg gactttacgg agagagaagt gtgatttggc agggaatcgg tgcctacact
    15301 ctgaccagtc aagagctctt ttatctgcta aatttggcta ttaagtacct ttgcactatt
    15361 cctctggcta ttatcttttt gatgacaacc caccctagtc agtttgcttc cagtttaaat
    15421 caaattggtg tgccttacaa gattgcctat tctgtcagtc taaccttgcg ctatattcca
    15481 gatttgcagg aagaattctt taccatcaag atgtctcagg aagcgcgtgg gatggaatta
    15541 tccaagaaag cttctcttat gcaacgaatc aaaggcaatc tgctcattat tactcccttg
    15601 atttttagct cgctagaacg cattgatacc attgcgactg ccatggagct tcgccgcttt
    15661 gggaaagaga aaaaacgcac atggtatagt tatcaggcct tgaaaaaagg agactatatt
    15721 accttgctct tggcaacctt gtttttagta gttagtttac tacttatctt gcagaatcag
    15781 ggacgatttt acaacccttg gaaatagctt gaaaaaattg aaaaaatcaa gtcatttcta
    15841 ttgacaatga ttctgaaagt gttatactaa gaaagtagtt tcgctgattt acttcaaacc
    15901 tgttgtgagg taagttaacg atgccttaac cacgctgttt gctgagcttg actccgggca
    15961 gtgtggctat ttttttgcaa tggtgaaagg aagcaagtca tgacaaatca cattgtatta
    16021 tttgaacctc aaattccaca aaatacaggc aatatcgcgc gtacttgcgc tgcgaccaat
    16081 tctcccctcc acatcatcaa gcctatgggc tttcctattg atgaccgcaa gatgaagcgg
    16141 gctggattgg attactggga taagctagag atttattttt acgagagctt agaagatttc
    16201 atgtctcaga tgaagggaaa actctatctg atttcgaaat tcgcggaaaa aatctattct
    16261 gaggtggatt tatcgactga cgaggaccat tattttctct ttggacgtga agacaagggc
    16321 ttacctgaag attttatgcg agaacatcct gagaaggctc ttcgtattcc catgaatgat
    16381 gaacatgtcc gcagtctcaa tgtgtctaat accgtctgca tgattgtcta tgaggctctt
    16441 cgccagcaga actttgcagg tcttgaactc gtccatacct atgaagtgga taaattgaaa
    16501 taaaaaatga aaatgaacaa aatgcttgcg cttgcaagcg ttttttgtta tgataaaaga
    16561 gtcttcaggg cagggtgtga ttcccgaccg gcggtgactt taactaggaa atgatctttt
    16621 ccttttatac tttgttgaca agctttgcct aaccagaagt tatgcctaca gcttgtcgcc
    16681 tagtctaaaa gaaaaatctc tatttccttc tctttaaaga agtccgcgag cgcaagctga
    16741 tgtggtgaga ttccacaacc gacagtatag tctggatggg agaagacgaa agaatagctt
    16801 tgtctgtttt gatagattta tagccggatt gtaaccactt gctttgtttt tctttaatag
    16861 agtaagaggg aacttttggg atataaaaag tgagaataga tagaggaatc ctttccaact
    16921 tcttctgatt ttatagaaaa ttggaggaac ctgttatgac aaacacacgt cgactttcga
    16981 ccattgcaat tctatcagcc atctcatttg tgctgatgta ctttgacttt ccgcttttac
    17041 cagcggcatc tttcctcaag atcgaattta gtatcttgcc agtccttgtg ggtctggtgg
    17101 tcatggattt gcctgctgct ctaggagtgc tcttgcttcg ctcactattg aaattgcttc
    17161 ttaacagcca aggagtgaat acttacattg gtttgccgat gaatatcgta gctttgggag
    17221 tttttgtcat cgtatttgct ttgatttgga aaaaggaacg gacaaccctt cgtttcctac
    17281 taggctctct agctggaact gttggtttaa ccttggccat gttggttctc aactatgttt
    17341 atgctgttcc tttgtacgct aagtttgcta actttgatat tggaaaaatt ttgggacttt
    17401 ccaactacct aatgaccatg gtattacctt ttaacttgat tgagggtgta atcttttccg
    17461 tttcattctg gttgttgtac gtccttttga aaccaacctt aaaacactat gaaagataaa
    17521 caaacatttt taatgaaggg cagttttgcc cttttacttt tcgttattct tggctatatg
    17581 gtcaaatttt accctgaaat gttggtcaat tttgaccaat cgattcagac tgccattcga
    17641 ggagacttac cagattattt gactattctt tttcgagccc tcacacgtct gattgatatc
    17701 ccagtgatta tcacttgggt tgtcattaca gcttttgtct tttatcgtaa gcgatggaag
    17761 atagaaagtt tcttcatgct gggaaatctg gctttggcag gtcttttaat cgtgaccttt
    17821 aaaaatatct accagcgccc acgaccagct attttacatc tggtggagga gaagggattt
    17881 tccttcccaa gtgggcattc tctagctgta acgctgatgg tagggacttt gattgttatt
    17941 cttagtcagc ggattaaaga tcaggtctgg agaaaaatcg tacaaatcgt ccttggcctc
    18001 tacctagtca gtgtgctggt atcaagagtc tatctgggag ttcattaccc atcagacgtc
    18061 cttgccagtc tctgtgtggg cttgggagtc ctgtttatcg agtttccctt ttatgacaag
    18121 ctccgcttcc aatggcgatt taaaggcaaa cagaagtgag tatcgaattc ccttgagaga
    18181 aagaaatgaa agtcaatata gcagatcttc atccgactca actatattta tcagaaaaga
    18241 agttgcaaga tattcagatg ctttaccagt cggcagaaac aatccaagtc gatccaatca
    18301 gtattcttgc ctttggagat tgtttgctga ttacagatgg gcatcacagg gcttatcagg
    18361 ctttattggc aggtcgggat acgatttctg ctgagtggga tagagatggt ggtgatgaac
    18421 tatatcatct ctatgcgcaa gcttgcgagg aaagaaagat ttactctgtt tttgatttaa
    18481 aaaatcatat cttacctctt tggatcggca tggtgagctt ttttggattg aagtcaagga
    18541 gaagggagaa tggtttccaa ttggggatat tacactatct caggataatc tccccattgt
    18601 gattgggaat tccgcttacc aacatcgagg acttggaaaa aagattctaa gtgctttgat
    18661 tgaattggct cgagtaaaag gatggaaaga attgagagtc aaggaaatct acacctacaa
    18721 tcatgcttct aggaggtgtt tcaagtcgct tggatttgtg gaaaatggag caacagaaaa
    18781 aggaaggagt tttatattgg aattagtcta atcctgccac tttctccccc ttaacctttg
    18841 actattccgc aaaattatcg taaaataaag agtaaatgat aaaatgaggt cagagtctgt
    18901 tcgctctggc gatagtagta taaatgagga gaaacgcttt ggaattagaa gtatttgctg
    18961 ggcaagaaaa aagtgaacta tctatgattg aggtagcgcg tgctatcttg gaacttcgtg
    19021 gtcgcgatca tgagatgcat tttagcgatc ttgtaaacga aattcaaaac taccttggaa
    19081 catcaaacag cgatatccgc gaagctttgc ctttgttcta cacagagttg aactttgacg
    19141 gtagcttcat ctcacttggg gacaacaaat ggggtcttcg ttcatggtat ggtgtggacg
    19201 aaatcgacga agaaatcatc gctcttgaag aaaatgacga cgatgaagta gcaccaaaag
    19261 ctaagaaaaa acgtgtcaat gcctttatgg atggtgattc agatgccatt gactacaatg
    19321 cagatgatcc agaagacgaa gatgcatacg aagcagatcc agctctttca tacgatgatg
    19381 aaaatccaga tgatgaaaaa aatgaagtgg aagcttatga tgcagaaatc aacgaaatcg
    19441 ctccagatga cttgggagaa gatgtggatc tcaacgaaga cgacgacgag ttttcagatg
    19501 acgatgctga aaccagcgag gaataagagt tagctattga caattatcct atttttaggt
    19561 atcatattgt ttgggcacct cttttagagg tcggggctcc ctagttctta gggagctatt
    19621 tttgtttttt caagaagtta tcttcttgta ttttatactc aatgaaaatc aaagagcaag
    19681 ctaggaaact agccgtaggc tgctcaaaac actgttttga ggttgtagat aagactgaca
    19741 aagtcaggaa cacatatcta cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa
    19801 gagtattagt tgtgaatctg gtgcagtcgt cccagattat tcttattagt agggtcttgt
    19861 tttctatatc ccctcgtagt taacaagacc ttgagcattt tagaaagagg aatctatgtc
    19921 tacgaaatat atttttgtaa ctggtggtgt ggtatcgtct attgggaaag ggattgtggc
    19981 agcgagtcta ggccgtctct tgaaaaatcg tggtctcaaa gtaaccattc aaaagtttga
    20041 cccttatatc aatattgatc cgggaaccat gagtccttac cagcacgggg aagtttttgt
    20101 gacagatgac ggagctgaga cagatttgga cttgggtcac tatgaacgtt ttatcgatat
    20161 caatctcaac aaatattcca acgtgacaac tgggaaaatt tacagtgaag ttcttcgtaa
    20221 agaacgccgt ggagaatacc ttggggcaac tgttcaagtc attcctcata tcacagatgc
    20281 tttgaaagaa aaaatcaagc gtgccgctct aacgaccgac tctgatgtca ttatcacaga
    20341 ggttggtgga acagtaggag atatcgagtc cttgccattc ctagaggctc ttcgtcagat
    20401 gaaggcagat gtgggtgcgg ataatgtcat gtatatccat acaaccttgc ttccttacct
    20461 caaggctgct ggtgaaatga aaaccaaacc aacccaacac tctgtcaaag aattgcgtgg
    20521 cttgggaatc caaccaaata tgttggttat tcgtacagaa gagccagctg gtcaaggaat
    20581 taaaaataaa ctggcccagt tctgtgatgt ggcaccagaa gccgttatcg aatcgttgga
    20641 tgttgaacac ctttaccaaa ttccactgaa cttgcaggca caagggatgg accaaattgt
    20701 ttgtgatcat ttgaaattag acgcaccagc agcggatatg acagaatggt cagccatggt
    20761 ggacaaggtc atgaacctca agaaacaagt taagatttcc cttgttggta agtatgtgga
    20821 gttgcaagat gcctatatct cagtggtcga agccttgaaa cactctggct atgtcaatga
    20881 tgtagaagtt aaaatcaatt gggtcaatgc caatgatgtg acagcagaga atgtagcaga
    20941 actcttgtct gatgcggacg ggatcatcgt accaggtggt tttggtcaac gtggtacaga
    21001 agggaaaatc caagccatcc gctatgcgcg tgaaaatgat gttccaatgt tgggagtctg
    21061 cttgggaatg cagttgacat gtatcgagtt tgctcgtcac gttttaggtc ttgaaggtgc
    21121 caattctgca gagcttgcac cagaaacaaa ataccctatc attgatatca tgcgtgatca
    21181 gattgatatt gaggatatgg gtggaaccct tcgtttggga ctttatccgt ctaagttgaa
    21241 acgtggctct aaggctgctg ctgcttatca caatcaagaa gtggtgcaac gccgtcaccg
    21301 tcatcgttat gagtttaata atgccttccg tgagcagttt gaggcagcag gttttgtctt
    21361 ttcaggagtt tctccagaca atcgtttggt agaaatcgtg gaaattcctg aaaataaatt
    21421 ctttgtagct tgtcagtatc accctgaact gtcaagccgt ccaaaccgac cagaagaact
    21481 ctacactgcc tttgttactg cagcggttga gaacagcaat tagcaaaatc agaacctttg
    21541 agaaaaatct cagaggtttt ttgcatacaa tgatattgca gtatatctga ggtaggagtc
    21601 ctctgtatgt acctgctacc gttgaaatta atagcgactc cctcttgccc tgtgctagtg
    21661 aatggattta tcagtatatt gaaatgaaat aaaatctgaa caaattaatt cggaaagcca
    21721 aatcaatttc tagcaaagtt ttaggaactg gattgtatag tgaattgaaa taagatatga
    21781 acatctctat cagaaaagtc aaattaat
//
LOCUS       DAVKLW010000035        20547 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKLW010000035 DAVKLW010000000
VERSION     DAVKLW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.035"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(96..245)
                     /locus_tag="VW324_001310"
                     /pseudo
     CDS             complement(96..245)
                     /locus_tag="VW324_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(399..2030)
                     /gene="rlmD"
                     /locus_tag="VW324_001311"
     CDS             complement(399..2030)
                     /gene="rlmD"
                     /locus_tag="VW324_001311"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9726947.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGLAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2104..3078)
                     /locus_tag="VW324_001312"
     CDS             complement(2104..3078)
                     /locus_tag="VW324_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9726948.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDSDKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3152..3766)
                     /gene="pmp23"
                     /locus_tag="VW324_001313"
     CDS             complement(3152..3766)
                     /gene="pmp23"
                     /locus_tag="VW324_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9726949.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLTLAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3766..4740)
                     /locus_tag="VW324_001314"
     CDS             complement(3766..4740)
                     /locus_tag="VW324_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9726950.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4748..6004)
                     /locus_tag="VW324_001315"
     CDS             complement(4748..6004)
                     /locus_tag="VW324_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9726951.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6066..6497)
                     /locus_tag="VW324_001316"
     CDS             complement(6066..6497)
                     /locus_tag="VW324_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9726952.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6499..7101)
                     /locus_tag="VW324_001317"
     CDS             complement(6499..7101)
                     /locus_tag="VW324_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9726953.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7085..7924)
                     /gene="prmC"
                     /locus_tag="VW324_001318"
     CDS             complement(7085..7924)
                     /gene="prmC"
                     /locus_tag="VW324_001318"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9726954.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7924..9003)
                     /gene="prfA"
                     /locus_tag="VW324_001319"
     CDS             complement(7924..9003)
                     /gene="prfA"
                     /locus_tag="VW324_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9726955.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9000..9590)
                     /locus_tag="VW324_001320"
     CDS             complement(9000..9590)
                     /locus_tag="VW324_001320"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9726956.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9713..9895
                     /locus_tag="VW324_001321"
     CDS             9713..9895
                     /locus_tag="VW324_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9726957.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10031..11404
                     /gene="mnmE"
                     /locus_tag="VW324_001322"
     CDS             10031..11404
                     /gene="mnmE"
                     /locus_tag="VW324_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9726958.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11451..12386)
                     /gene="dapA"
                     /locus_tag="VW324_001323"
     CDS             complement(11451..12386)
                     /gene="dapA"
                     /locus_tag="VW324_001323"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9726959.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12442..13518)
                     /locus_tag="VW324_001324"
     CDS             complement(12442..13518)
                     /locus_tag="VW324_001324"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9726960.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14104..14176)
                     /locus_tag="VW324_001325"
     tRNA            complement(14104..14176)
                     /locus_tag="VW324_001325"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14141..14143),aa:Thr,seq:ggt)
     gene            complement(14276..14785)
                     /locus_tag="VW324_001326"
     CDS             complement(14276..14785)
                     /locus_tag="VW324_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9726961.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14783..14879)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14908..15339
                     /locus_tag="VW324_001327"
     CDS             14908..15339
                     /locus_tag="VW324_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9726962.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15421..15798
                     /gene="mscL"
                     /locus_tag="VW324_001328"
     CDS             15421..15798
                     /gene="mscL"
                     /locus_tag="VW324_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9726963.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15840..16934)
                     /gene="hemH"
                     /locus_tag="VW324_001329"
     CDS             complement(15840..16934)
                     /gene="hemH"
                     /locus_tag="VW324_001329"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9726964.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVTLVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17089..18309
                     /gene="pepT"
                     /locus_tag="VW324_001330"
     CDS             17089..18309
                     /gene="pepT"
                     /locus_tag="VW324_001330"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9726965.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVRSL"
     gene            complement(18352..18981)
                     /locus_tag="VW324_001331"
     CDS             complement(18352..18981)
                     /locus_tag="VW324_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726966.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVAA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            18946..19167
                     /locus_tag="VW324_001332"
     CDS             18946..19167
                     /locus_tag="VW324_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726967.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVFLKQESYNPRLQ
                     GLTEIRNPDKTMSVQDAIRFAQQRGFNML"
     gene            complement(19244..19891)
                     /locus_tag="VW324_001333"
                     /pseudo
     CDS             complement(19244..19891)
                     /locus_tag="VW324_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19995..20547)
                     /locus_tag="VW324_001334"
                     /pseudo
     CDS             complement(19995..20547)
                     /locus_tag="VW324_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5918 a         4325 c         3884 g         6420 t
ORIGIN      
        1 ctatttattt tttgagtttt aaactttggt agaagtcttg aactccttat atttacataa
       61 ctatttgcct ctataactgt tatgacacga aaccaacaag caagttcaag cacctccata
      121 tctttattca aatgaggact aagatattca caaactttat cataaacctc tttatctttt
      181 ttcataaaag gggcatacaa cttagcaaat ttatcctaaa attttttgtt gcctttatca
      241 atcatttcaa cgctccttta aatataaaaa tgatcctatc aatctttata ttgagcctat
      301 tttctaattg ataccttgca agaaaggaat aatagctgtt cctataccaa gtaggaatgt
      361 aataaatgca gaaagtcata tactgtcatt cttttctatt aaatcatttt gaagtgtctc
      421 aaagcatcca tgatggcttc ttctttttca ggtgtacact gtggaataat ttgtttatcc
      481 tttttagact tgttgtaatg ttctcgtaat tctattccac atttcttttt tatctgtgca
      541 atatataatg tcgaaacttt taattcaaat ttattccaaa catattcttt gatttgagca
      601 tatgttgctt tgctctccgc acttgtcaaa tccatctcat ccagctcaat ttcaacactt
      661 atgtgcttat cgacatcgag tttggacaaa agtgctaccg tctcgacgtg atgcgtttga
      721 ggaaatagat ccaccggctg gactttcttc aattcatatc ccaactcttg gtatagttta
      781 atatcacgcg ccatggttgc gacattgcag gagatatagg cgatgcgatc ggctcctgtt
      841 tgggcgcttg ctttgataaa gctttctgtc aagcccttgc gtggaggatc aaccaagata
      901 acggttggtt gaatcccatc tttaagccaa ttcttcatag cattttcagc tgtgtcacag
      961 acatagtggg cgtttgaaat attgttcagc tgagcatttt ttttactatt ctcaaccgct
     1021 tctggaatca gttcaacacc gtagacttct ttgacatgct tggcgactga taaaccaatg
     1081 gttccaatac cagaataagc atcaataacc acgtcatctt tttttaactc tgcaaagtca
     1141 atggctgttt gatagagttt ctccgccatt tcagtattga cttggtaaaa ggctaggcca
     1201 gcgatttgga agtcatttcc caacatctgg tccgtaatat agtcttgacc ataaagagtg
     1261 cgccactcct taccaaaaat cgcattggta ttctggtcgt tgatattttg catgacagac
     1321 acaatctctg ggaactgctt gataacttgt tcaatcaatt ggtcaacacg aaaaactttt
     1381 ggacgagttg tcaccaaaac gaccatgatt tgtcctgaat agtgaccacg acgcaccaca
     1441 agattccgaa tcaatccaga ctgttccttt tcgtcataag gttttaaatc aaaacgacgg
     1501 agcaggtctc gaagagctac tacgacttgg tcaatgacag gatcctggat aaagaaatct
     1561 tcaaggggca tgaggttgtg cgaattctta cggaaaaatc ctgtttccaa gacaccattc
     1621 actcgacgaa cgggcacctg cgccttattg cgatacttga ctggatgttc cataccaagc
     1681 gtttcagcaa cttctacatc tgcaattcca gcaatcttgt agagactgtc cttgacttgc
     1741 ttggttttaa acttgagctg ttctggataa gaaaggtgtc ctaaatccgc gattcctgaa
     1801 cgcaggtaag ccaaatctag atcttgatta cggtgtggtg actggacaag gtatttttca
     1861 acttttccaa agccaatctt tttattgacc ttgaggacac gcatgagaat tttttcactc
     1921 ggtaaagcat tctctacaaa aaagaccaaa ccatctacct tggcaactcc tgccccttca
     1981 tgggtcaaat caacaatttc aacttctaca atatcatttt tctttaacat tcttttctct
     2041 ttctattggc cgacaaaaaa gacggcaacg ttacggttac catccattat accatgaaat
     2101 ttcttaagaa ccaaaactct tgaagaagtt gacaagggct tgccagaggg agcttaagaa
     2161 gttaccaccg tcctctagcg ccttatccga atcaaagtta aggttgatat ttttaaaact
     2221 gtcgccagct tgtgatacga tgctttgttt aaggtcattt aaggttttag tgaaatctgc
     2281 attgctgagg atatcactct ttgagagatt caaggcaaaa ttgatgatga tattgatctg
     2341 gtttcctgtt atgacctgat caagtttgta attttttaag gtatcttcaa caatcttgcg
     2401 gatatcttct tctgtcagat ttcccttgct ttctttagct ttggcgagtc ctgacttgat
     2461 atcagctagg gcaacgttta atttattagc atcatagcct gatttgtcct tgttttcagc
     2521 attgatatct gacaaagcct ttagctcttc ttgagccaaa tctttattgg cttgcggcac
     2581 cttggctcca ttagcttcta gcgaatagta gatcccagcc aaagcactct cacctgtaac
     2641 tggaatgggt gctgctacag tgattttggc gtgttccaca cccaaagtta cggctgcatt
     2701 tcggtacata tcctgagtta ccttagtgat gttttcaggt gtttcaatct tgacctcaag
     2761 tggcgatttg tcacctagct tttgaatctt ggctgatgaa tacaactgta agctagagtc
     2821 attggccaca ttcatgattt tagaataaac atcaggtgtc atggtcttga gttctttggt
     2881 atctgttgag gcattgtagc ccagtttttt aagagtttga tttttttggt cttcagatag
     2941 ggaagaacct aggacatatt caggttggac ataggtttca tcgataactt tttgaacatc
     3001 tgttgctgca tggacgctat tcatagctgt tactgcccac aagatcgcag cgctagtcag
     3061 aaagagtttc tttctcatag ggaatttcct cctttacttc tttagagtaa tatatctatc
     3121 ttaaagaaaa cttataacaa aaacacctgg tctagccaga tgttgaaaag agagtgaaac
     3181 atttgatgat gtaaaggtta agtcgtacct gtctagaata ataatagttt cctccattta
     3241 catagagttc agcaccgtga aaaatggaaa tggggtgaat ataactataa gtctttccag
     3301 tcctattacc aagcaagggg gcaacagtct cacgagagta ctgtttggct agagtcaagg
     3361 tattttcctt gccattttgg gcgataaaat cgatataggc aggtccaaaa ttataggctt
     3421 gaacagctgt ccagatatct acacccttct tctgcgccag atagagattg cctgtcagag
     3481 tttgaatgcc ttgccgaatg ctagaggcat tatcattgat ggtgttggtg gaaccacttg
     3541 cagactcact agactgcata acatcgcctt cttttccttt tgtttcagta taaatcatag
     3601 caagcacaag ctcttcgttt gctggggtgt ctttttcact caatatttct cgcaccatgg
     3661 gttgataggt catgacttgt ttgacatctt gatgaacgcg gtaagcttta tagccagcaa
     3721 aaaggaagac tgctagtaca agcactcttc gaattcgttt aaacattatt tactttggat
     3781 atcctcgata tttttgatta agatagagta ggttccattt tcgttttgga taaactcaac
     3841 agactcggcg tcttgataga cgttattggg aacgatgagc tcaattccat ttgataagga
     3901 gagtttttgg ttttcaaatt tctttaattg gcgactggca tcaatttcat caaattgaac
     3961 aggttctggt acggcttctt tgacttggtc aataaagctc aaacgagccg tcagattgtt
     4021 gtcaaaaagg tcattagcca atttctcagg tgacaattca ttgctttctt ctaggttgtt
     4081 gaaaatagct gatttgacct tggattgaaa ttgaaaatca tctgtgttaa aagattcagc
     4141 aattctctgg gctgtttttt ccagttcctt gatagatttt ttaggagaaa tcttaggagc
     4201 gacagcaaga agattatctg aaaaatagtt caaaaaagtc ccgttgtact tgattcgttt
     4261 ttcaatcagg tgatacttgc gactctgaag attgaccacc aaggcctcgt cagcacccgt
     4321 tccaaatcca ggcaggttat tctgagtcag cttgattgga ttatcaactt ctcctccgag
     4381 gtgggtcaag gtctcccgca gggcaattcg caagaaagcg aaatgttcta caccttcttt
     4441 agaaaattga acaaaaatca agtcattggt cttgagattt tcagaaatgc taaactcctc
     4501 tttccagaga ttagccagcg ttactgatgt ctccaacaaa tcgtctgtaa tatgattgaa
     4561 gaagggattt tcttcttcga aaatcccagt cttggcttca tctgaataca catgttcaat
     4621 ttttttacgt aggtattctt cgatttttgg agtaatattg agaaacttat ctgctaagaa
     4681 cagctcggta tcatccggac tgaactggtg aataatggct ttcttaatat aaatgtccat
     4741 aaaagtttta ttcctcgtat aatgggaagg catctgtcaa ttctttgact gcacttctca
     4801 cttcttctaa tacagcctca ttttctgaat tcttaagggt tttaatgatg agttcagcca
     4861 ctttgcgact ttcttcttca ccaaatccac gtgcagtaat agctgctgct ccgatacgaa
     4921 tcccacttgt cttgaatggt gacaaggttt cgtaagggat tgaattttta tttagggtaa
     4981 tattgacttc atccagcaag ttttgagcaa cttttccgtt ttctacaacc ttagtcacat
     5041 caacaaggaa gagatggttt tcagttccac ctgaaataat acggaaatca gggtcttgca
     5101 agaagacatc tgccatagcc ttgctgttct taattacatt ggcagcatat tccttgaagg
     5161 ctggatccaa aacttctttg aaggaaactg ccttagccgc cacaacatgc tctaaaggac
     5221 cgccctgaat acctgggaaa atagctgaat tgattttttt agcaagttct tcgtcattgg
     5281 tcaaaatcaa accaccacga ggtccacgaa gggttttgtg ggtcgttgtt gttgtgatat
     5341 gagcgtatgg aactgggctt ggatgaaggc cagccgcaac caaaccagcg atatgggcca
     5401 tgtccaccat gagcttcgca ccgacagcat ctgcgatttc acggaatttt gaaaaatcga
     5461 taatttgaga atatgctgaa gcaccagcta caatcagttt tggttttact tcttgggctt
     5521 gtttcaagat agcatcaaag tctaagagtt ccgtttcagg atcaacacta taagaaacaa
     5581 agttgtaggt ttgaccagag aagctaacag gagccccatg agtcaaatga ccacctgatg
     5641 ccaaatccat tcccataacc gtatcacctg gctcaatcaa ggacatgtaa gccgcacagt
     5701 tagcttggct tcctgaatgt ggttggacat tggcaaattt agcaccgaaa atttcttttg
     5761 cgcgttcaat agcaagagtc tctacaacgt ctactacatc agttccacca taataacggc
     5821 gtcctgggta accctcggca tatttatttg tcaagataga cccttgagct gccataacag
     5881 ccttggaaac tacgttttcc gaagcaatta actcgatatt attttgttgg cgttcttctt
     5941 ctttggcaat agcattccag agatcagcat catatgcttt aaaatcatct ttgtcaaaaa
     6001 tcataggtct tctcctttat tgtgtgacta gtccattagt ttgattttac aataagaaaa
     6061 tcaaactaaa agatgcgaat aaaccgtttc tgcattttat cacaagtata gccaactttt
     6121 tcataaaatg catgagcacc cagacgatga ttggcagaat ttaagcggat aaacccataa
     6181 ccacatcttt ttgcttcttc ttccaaccct tgtagtaaac ttttaccaat accttgacct
     6241 tgcgcttgag gtgaaactgc taaagctaag atattaaatc ctgctttgga atagagtgat
     6301 tcgtaaactt cagcgtggac atatccaagt aagacatgat tagctgcatc ctcatagcca
     6361 agtaggaaat gatgggaatc ctgagacagt ctagctagtt ggctagccgt ttcctctgga
     6421 ctaaaagtat aacccaaagc ctcttggttg atgtcacata tcgctttcac atctgtttct
     6481 tgcaaatctc ttagcatttc aagcctcctc aaaagaaatc tctggcaacc gagcaagaat
     6541 atcttctcgt ttaattgcgc cttggcgtaa gattttcacc ttgtctccag acaaatccac
     6601 aatagttgaa tcctgtccag ttagaaaagc atcgtcttcc agacccagaa cctcttggtc
     6661 aaaatccttc agaatttgtt caaaggttac accacttgcc tgacctgaga tattggcaga
     6721 cggcccaatc aagggacctg tctctcgaat taaatccagt gtgatagggt gactgggcat
     6781 ccgaaatcca atagttgcaa ggtcagaatt tacccaatag ggaactcggt cattggcttc
     6841 gagaataatg gtcaagggac ctggcaaaaa ggtctctaca agtttttgta gataagttgg
     6901 ctgattctta gaaaagtgca agatatcctc gaaagaggcg atattgagat tgagtgcctt
     6961 atctctagga cgacgtttga gttggtaaac atggtcaact gctttttcat ctaaggcctt
     7021 ggcaaaaaga ccataaacag tctctgtagg tagaacgaca gctccaccat tttccaactc
     7081 ttgtctaatc ctgtccatca tcaaccacaa ccatcctatt ttgaccaaat tggtctttga
     7141 gtgttcgaac ttttttttca ggaagatgtt ttctaaaaag ttcgggaaca ctttgacctt
     7201 gcttgtatcc aatttcaagg taaatcttac caccatcctt gagatagtct tttgcatctt
     7261 ccgcaattct acagtaaata gctaggccat cctcgtctgc aaagagagct agatgaggct
     7321 ccgaatgcaa aacattcaaa cctacctctg actcatcttc acgagagata tagggtggat
     7381 tggatacaat tatatcatat ttttcagaaa tttctgcaaa acaatcagat tttttaaaaa
     7441 atatattaag attttgatcg ctagcattct ctgatgcaag ctctaaggcc tcttgagaaa
     7501 catctgctgc tgtcactgac caatctggtc tattttttgc tagagcgaga gcaatagctc
     7561 cactacctgt tccaatatcc agaactgaga gattcgtctc aagattttca gttagaataa
     7621 gctccactaa ctcctctgtt tctggacgag gaatcaaaac ccgttcatcc acctttaaat
     7681 gcattccata aaaatctgcc tgtccaatga tgtactgagc tggcttgtga gctgctagtt
     7741 gctggtaaat atcttctaca aattgttttt cttcctctgt tgtcacctcc tgctggaggg
     7801 caaaaataaa gtctgtaaaa gatagatttt tcagactacg atagacaaaa gagaggcttt
     7861 ccgcttcctc tccttgtctt atcaactctt cttcaaaatt tgaaaataat tgagctaatt
     7921 tcattatttg tttaattctt ctagtttttg tgtttggtca taaagcacca aggcatccac
     7981 aacttcgtcc aatttaccag acaaaatcgt atctagtttt tggagggtca agccgatacg
     8041 gtggtctgtg acacggtttt gtgggaagtt ataagttcgg atccgttctg aacggtcacc
     8101 agtaccgatt gtcgacttac gctcagcgtc ttgttcatcc tgagcaatct gagcaaagtg
     8161 gtcagcgacg cgtgcacgga taatcttcat agccttctcg cggttcttct gctgggtacg
     8221 ttcttcctgc atctcaacct tgatattggt tggcaagtga acgatacgaa cggcagtcgc
     8281 aaccttattg acgttctgtc caccagcacc agaggcgtga tagatgtcga cacgaaggtc
     8341 ttttggatca atgtcgtatt caacctcttc aacttctggc ataacaagaa ctgtcgctgt
     8401 cgaagtatga acacggcctt ggctttctgt cacaggaaca cgttgcacac ggtgggcacc
     8461 tgattcatac ttaagcttag agtatacaga ctgacctgaa accatagcaa ccacttcttt
     8521 aaaaccaccg acaccattca tagaggcttc catgacttca aagcgccaac cttgggcttc
     8581 cgcatacttt tggtacatag ttagcaaatc tccagcgaaa agtgccgctt cgtctccacc
     8641 agctgctcca cggatttcaa ggatgatatt cttgtcatcg tttggatcct ttggaaggag
     8701 caaaattttc agtttttctt catattcttc tttttcagcc ttggcatctt tgagttcttg
     8761 cttggccatt tcttccaagt ccgcatctcc gcctgattcc ttaatcatct cttcggcatc
     8821 gacgatattt tgaaggactt gtttatactc acggtaggct attacggtgt cacgattgga
     8881 agcttcttct tttgaaagct ccataaaacg cttggtgtct gaaacgacat cagggtcact
     8941 cagcaattct cctagttctt cataacggtc ttctacagct tgtagttgat catagatgtt
     9001 catttttctt cattctcctt attgatttca ggggcaaaat aatgtttacg gcaaaccgag
     9061 atataggttt cattaccacc aatctggatc tgttctccat cgtaaacggg cagtccatcc
     9121 tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt cttgatttcg
     9181 tcaatcttgt ctgctaaaag caagagatat ttggaacctt cgaacaattc attgcgaaag
     9241 tcatttttca agccaaaagc catgacgggt atgtctaact cgtccacaac acgagctagg
     9301 tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaaaacaca gtaaggtttt
     9361 tctggtaggt ctcggatata gccaaagata tccgttgttt cctcaatcgc aagggcaggg
     9421 cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc cagagccgag
     9481 gtcataatca caacaccttt tccttgctcc tcgtagttat aggccacttt gagaatctca
     9541 atcgttttac cagagttcat ggtcccataa cgatagtaca actgtgccat gtttcttgct
     9601 tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct taagctttaa
     9661 acggcaaaat gtggtaaaat ggaagaaatc aaaactagtg gaggaagcta ttatgccatt
     9721 tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc ttgctaagga
     9781 agtaacggaa gcggttgttc gcaacactgg agcacctcaa tctgctgttc atgtcatcat
     9841 caacgacatg ccagaaggaa cttatttccc acaaggggaa atgcgcacca aataagctag
     9901 cttaagcaga attgcttagg ctttttcaat ctccaagtag cacccattga agaaatagtc
     9961 taaatttgtt acaatttgaa aagagacttg gaaaattcca agaaaagagc aattaattaa
    10021 aggaaacatt atgattacac gtgaatttga taccatcgct gctatctcta ctccactagg
    10081 tgaaggggct attggtattg tccgcctgag cggaacagac agttttgcta ttgtgcaaaa
    10141 gatttttaaa ggaaaagact tgaacaaggt tgccagccac actctcaact acggtcacat
    10201 tattgatcct ctgactggta aagtcatgga cgaggttatg gttggggcta tgaagtctcc
    10261 aaagaccttc actcgtgagg atattatcga gattaacacc cacggtggga ttgcggtgac
    10321 caatgaaatt ctccagctag ctattcgtga aggggctcgg ttggcagaac ctggtgaatt
    10381 taccaaacgt gcttttttaa acggtcgcgt agacttgaca caggcagagg ctgtgatgga
    10441 tatcatccgt gccaagactg acaaggccat gaacattgcg gtcaaacaat tagacggctc
    10501 cctttctgac ctcattaaca atacccgtca agaaatcctc aatacacttg cccaagttga
    10561 ggtcaatatc gactatcctg aatatgatga tgttgaggaa gctactactg ccgttgtccg
    10621 tgagaagact atggagtttg agcaattgct aaccaatctt cttagaacag cccgtcgcgg
    10681 caaaatcctt cgtgaaggaa tttcaacagc tatcatcgga cgtcccaacg ttgggaaatc
    10741 cagccttctc aacaacctct tgcgtgagga caaggctatc gtaacagata tcgctgggac
    10801 aacacgagat gtcatcgaag agtacgtcaa catcaatggt gtacctctca aattgattga
    10861 tacagccggt attcgtgaaa cagatgacat agtggaacaa atcggtgttg agcgttcgaa
    10921 aaaagccctc aaggaagccg acttggttct actagtgcta aatgccagtg aacaactgac
    10981 tgcgcaagac agacaacttc ttgaaattag ccaagatacc aatcgcatta ttctacttaa
    11041 taaaaccgac ctgccagaaa cgattgaaac ttcgaaacta cctgaagacg ttatccgtat
    11101 ttcagtcctt aaaaaccaaa acatcgacaa gattgaagag cgaatcaaca acctcttctt
    11161 tgaaaatgct ggcttggtcg agcaagatgc tacttacttg tcaaacgccc gtcacatttc
    11221 cctgattgaa aaagcagttg aaagcctaca agccgttaat caaggtcttg agctggggat
    11281 gccagttgat ttgcttcaag ttgacttgac tcgtacttgg gaaatcctcg gagaaatcac
    11341 tggggatgct gctccagatg aactcatcac ccaactcttt agccaattct gtttaggaaa
    11401 ataagaaaaa tccatgatcc ttcattcggt catggatttt attgtcttta ttagtaatct
    11461 ggtcttaaga cccctgttac agttgcctta gttgcttcgt agtcgccatc tacgacaacc
    11521 ttgataatgc gtttggcatc ttcttctggt gctggaacaa gaggtagacg agtgggtcca
    11581 gcttcaaatc ccatatagtt aagaattgcc ttaactggag caggacttgg ataagagaag
    11641 agagcattaa ccttaggaat gaatttacgc tgaattgctg cggctttctt catatcgctt
    11701 tctgcaatgg cagtaaacat ctcgtgcatt tcatccccat ttgtatgaga ggcaacagaa
    11761 ataaccccat ccgccccaag gttcatggca tggaaagcat ctccatcctc acctgtataa
    11821 atcaagaact cttcaggctt gtgctcaatc aagtaagcca tattagccaa gctagtacat
    11881 tctttgacac cgataatatt tggatggtca gccaagcgaa gcatggtttc tggagtcaat
    11941 tcgacaacta cacgccctgg aatgttatag ataataattg gtaggtcaga agcatctgca
    12001 atagccttaa agtgctgata catcccttct tgagaaggtt tgttgtagta aggaacaata
    12061 gcaagcccag ctgcgaaacc accaaattcc gctacttctt tgacaaactc aatagagtca
    12121 cgcgtatcat tagtacctac acccgcaatc aaaggaacgc gtccattgac aaccttttgt
    12181 acagccgcaa acaactccaa ctcctcatcg tgggtcaaag ttggactctc agcagtcgtt
    12241 cctgcgagaa gaattccatc cgtatgatgg gccaataaat gctcaatcaa ggctggaata
    12301 gcatcaaagt taatggaacc atcctcatgg aagggggtaa taaaggctgt aatgatttta
    12361 cattctttta aatcttgata agacacgaaa acacctctct atttcaaaga aggagttcat
    12421 ctgaactcct aaacgatatg actattttaa ttcaaatttc aattcagctg ttggacgaac
    12481 caatccacgt tcatgaagag tttcagcaat ctgaactgag ttccaagcag cacctttgag
    12541 aaggttatct gaaacaaccc acatgtgaat tcctttttct gcatccaagt ctttacggat
    12601 acgaccaaca aaggtatcac gtgaaccaac tgcattgatg gcttgaggat agatttgatg
    12661 aactacatca tcttcaagaa cagcacctgg gaaggatgcg atagctgctt tgacttcttc
    12721 aattggagcc acttcctttg tttcaatata aactgactca gagtgagctg acaagactgg
    12781 aatacgcaca catgtcgcag atactgcaat gctatcatct tccataattt tcttagtttc
    12841 cttggtcatc ttcatctctt cgtacgtgta atcattatca gtgaagacat caatttgtgg
    12901 aagagcgtta aaggcgatag gataatgttt cttgtcacca cctgaaggca agatttccgc
    12961 atgcaaatca cgtggtttca ctccatcatt caagacctca cgaagttcac gttgtgtctc
    13021 aagaattgct cccataccag cacctgaaac ggcttgataa gttgaaacaa tgatacggtc
    13081 caagccccat ttttggcgaa ccggctcaag agccaccatc atttggattg ttgaacagtt
    13141 agggcaggca atgattccgt tgtgggcatc aagtgcatga gcattgacct ctggaacaac
    13201 caaaggaaca tctggatttt gacggaaata agatgtatta tccactacta ccacgccagc
    13261 ttttactgcg tatggtgcat acttagctga tgtcgaacca cctgctgaaa agagagcaat
    13321 atcaactcct tcaaaagctg tttcagtcgt ttcttcaatc gtaatatctt gatctttaaa
    13381 tttcaatgac ttacctgctg aacgtgcaga agcaaggtaa cggattttat cgattggaag
    13441 tgttgattct tccaacattt ttatcatctg agcaccgaca gcacctgtcg cgccgactac
    13501 agcaactgta tatcccataa ataacctctt tcggaatttt ctaaaaattt ctataataga
    13561 agtattatac tactttttcc atgaattgca aagctttttc atcatttttt ggaaaataaa
    13621 aatccccaat cgctagacta gggattaaga ggcatcttca tgtgatgagc aggttcacac
    13681 aactcatcaa ggtccgctcc tgcgttatga cctccttatg ctcaatagta gcccgaagac
    13741 tacctatcga ctcatcgcat ctactattct actaaagaga atgacttttg tcaacagacg
    13801 aaactcttta tactcttcga aaatctcttc aaacttcgtc agcgtcgcct tgccgtatgt
    13861 atggttactg acttcgtcag tttcatctac aacctcaaaa acatgtttta agctgacttc
    13921 gtcagttcta tctacaacct caaagcagtg ctttgagcaa cctgcggcta gtttcctagt
    13981 ttactctttg attttcattg agtattagtt ttatttacac aggtgcataa gaaatatatt
    14041 ggaaaaattg ggggcagaca taacaaaaac ccttgtaaat caagggcttt tctgtctatt
    14101 taatgctaat tgccgggatt gaaccggcga cctcatcctt accatggatg cgctctgcca
    14161 actgagctaa atcagcttac ttaagaagta tactatattc agaaaaactt gtcaagtttc
    14221 cttagaaatt ttccatagga aaaagccagg taagaactac ctagcttatc ttcttctatt
    14281 tacttaaatc aattcgacga ccaattttac caataataat cgctccaata atcaatgaag
    14341 caaattcacc gattcctgtt gtaaaccaag taaggaagaa tggtaattct gctacaatat
    14401 ttaactctgc agcgatagta aacatggaaa ttgaaaagag gattgaaaag aagaaatgat
    14461 cttttcgaat caatccgtta aagaggtaat ccttgctata ttttgcaaaa agccaaacac
    14521 ctaaactgag gaatactaag gttgaaccac caccaacaaa gacatctagc agtccgaagc
    14581 taaagaaatt agcaatcata caaccgatgg taactccgat gatgtactta ggattgtaaa
    14641 aagccataaa gttcatcatt tctgaaatac gaaactgata agcaccatag ctgatggcat
    14701 ttaggggcgg tgtgacagtc aaaacgacgt agatagcagc gacgattgca atatctgcaa
    14761 catcacgaat agttaatttt ttcatctttt ctcctttggc ggttatccgc gtgtaatatg
    14821 cttggtgaaa gaagctaagc accaagggtt gattcattca accttactag tatagcacaa
    14881 tagcctgctt tatactatac ttaaagcatg aaaaaaccta ttcaaaaatt ttttaacaat
    14941 gaaatcttag cctatctctt ttttggcctt gcgacaactc tagtttcgat tctctcacga
    15001 ctagtcattt atcaactaag tcatcaggaa ctccttgcta cggccttggc aaatattatc
    15061 ggtatcttat ttgcctttat cacaaatgat acgattgtat ttaagcaaaa aaagaaaaat
    15121 agacttatcc gtttagtgaa attttctctt gctcgccttt ctacttttct gttagacttg
    15181 tttttaactt ttctctttgt aacccaattt cctcacatca tagggcaatt cgtaactgaa
    15241 aacattgata agataaatgc tattgaaaca ataattgcac aaatattcat tattatcatc
    15301 aattatatct tgagcaaggt ctatattttt agaaaataat gctccattta tgagcctttt
    15361 aattgccttc tataatgatt taggatacaa tgaaacacga aacattgaaa ggaaaacagt
    15421 atgttaaaaa atctaaaatc gttcttgctt cgaggaaatg ttattgacct tgctgtcggt
    15481 gttgtcattg cctctgcttt tggtgctatc gttacttcac ttgtaaacga cattatcact
    15541 cctcttattt taaatcccgc tttgaaagct gctaaagtcg aacgtatcgc tcaactttct
    15601 tggcatggag tcggctatgg taacttttta agtgctatta tcaattttat ctttgtgggt
    15661 accgccctct tctttattat caagggcatt gaaaaagcac agaaactgac tggcataaag
    15721 aaagaaaaaa ctgccgaaaa aaaaccaacc gaattggaag tccttcaaga aataaaagct
    15781 ctccttgaga aaaaataatc gattaaaagg atctagcgca aagctaaatc cttttttctt
    15841 tactctttgg gtaacttgcc tgctttttca agcatttttt tgataagata aggcatctta
    15901 acattctccc gccctttttt ctcaatcagt tttttcacaa attctggcat ttgtaaatct
    15961 tcagattcag ctaaaatgtt tttcgtctcg tctgaaggaa ctaactcatc aaaggtctct
    16021 tcttctggga gaaattcctt aaactcttga ttttcattga ctctaactgt attgaccaaa
    16081 gcatcaatca aacgagaatc tgtatttggc attggtggac gatggtagtt taccccaaat
    16141 tcctggcata agtcataaca ttctacatca ttgtcaaaca agacctcaat atgctcactg
    16201 atgaaactaa taggtacaaa aatataatgg tctggatgtt ctgtctgttc tctgagatac
    16261 tccaaaacat ctggtttaat ccatggaata ccaatatcac tctcactctg ccaagtgttg
    16321 gtatattgtt ctgaactcaa gcccagtttt tcagcgacta gcttgctatt ttcgaaaatt
    16381 tggtctatat aaggatcacc aaagtccaag gcaaaaatgg gcacactatg ggctgaaaag
    16441 atgactttaa agctgtcttg ctttacttct tcttttaaaa tcttagcaat ttcatctgcc
    16501 caatagttta agagcgcttc ttcctgatac cagtccttaa tgaccaaaaa ttgaatttgt
    16561 ttgctttcta aaaatttttc atagcccatg acagagtaga aagaataatg gggctccaaa
    16621 atcaagcaaa tacactgttc aattccgtct gcttccattt gaccgatcac atctggaata
    16681 aagggactgg aaaacttatt agcaaagtag acactgtatt cattccccag cctagcttct
    16741 accaaggtaa cctcttgacg ggtaattttt tgcagaggcg tgcctccaat tcgtacataa
    16801 ttatcataga gagtctgaat ctcgtggtct tggggtctca ccccacgacg aatatttgtg
    16861 aaaaaatcag ctacaccttc aaaggtaatc tcttctggtg aaccgaaggt catcattaaa
    16921 attgcttttt tcatgactgc ttccttgtga tgtttttatc aagtttattt tatcattaat
    16981 tcatcgataa gtaaacgcta acatagggac attctcaatc ttctgtcttt tgtttttctc
    17041 ttctttctat gatacaatgg aaaaaataaa ttcaaaagga gtttttttat gacttatcca
    17101 aatctcttgg accgcttctt aacctacgtt aaggtcaaca cgcgctctga tgaacactct
    17161 actactactc caagtacaca gagtcaggtt gacttcgcaa caaatgtcct aattcctgaa
    17221 atgaaacgtg ttggactgca aaatgtttac tatctaccga atggttttgc tattggaacc
    17281 ttgccagcca acgatccgtc tttaacacgt aagattggtt ttatatcgca catggatact
    17341 gctgatttta atgctgaagg agtcaatcca caggtaattg aaaactacga tggtggtgtg
    17401 attgaactag ggaattctgg tttcaaactc gatccagctg acttcaagag tcttgaaaaa
    17461 tatccaggac aaacgctcat cacaacagat ggaacaacct tgctaggtgc tgatgacaag
    17521 tcaggaattg ctgaaattat gacagccatt gaatatctaa ctgctcatcc tgaaattaag
    17581 cactgtgaga ttcgtgttgg ttttggtcca gatgaagaaa tcggtgttgg tgccaataaa
    17641 tttgatgcag aagattttga tgtggatttt gcctacactg ttgatggtgg tccactaggt
    17701 gaacttcagt acgagacttt ctcagccgct ggtgctgaat tgcatttcca aggccgtaat
    17761 gtccaccctg gtactgccaa agggcagatg gtcaatgccc ttcagctagc aattgatttt
    17821 cataatcaac ttccagaaaa tgaccgacct gagttaactg aaggttacca aggtttttac
    17881 catctaatgg atgtgacagg tagtgttgag gaggcgcgtg caagctacat cattcgtgat
    17941 tttgaaaaag atgcctttga agcgcgtaaa gcatccatgc aatctatcgc tgataagatg
    18001 aatgaagaac ttgggagcaa ccgtgtcact ctcaacttga cagaccagta ctacaatatg
    18061 aaagaagtca ttgaaaaaga tatgactcca attaccattg ctaaagccgt tatggaagat
    18121 ctaggtatca cgcctattat cgaaccaatc cgaggtggaa cagacggctc taagatttcc
    18181 tttatgggaa tcccaactcc gaatatcttt gcaggtggcg aaaatatgca cggacgtttt
    18241 gaatacgtta gccttcagac tatggaacgt gcagttgata ccatcattgg catcgtacgt
    18301 agcttataaa agctaaaaag acgaggtagc tcagctactt cgcctttctt tttattctac
    18361 tggtttttct tgatttccag tagttgtaga agattctgtt gttttatttt ctgaagttga
    18421 ttcagcaggt ttagaatctc ttgtattgct tggtttgttt tcgtcgctag cagtttcaat
    18481 gttagattct gcagttgcgt ttggttggtt ctcagcactg gtgttatcac catttgcttc
    18541 agcatttctt gctggacttg tttcttcact tgcgctagct tttgactgga tttgatgatt
    18601 caaaactaga atagcttttg tcgattcaag taaagctgtt ttgtctttac tcttagcaga
    18661 aagttgatct aataatgcag ccaccttatc aaagtccgca tcagatccat tattactttc
    18721 taaataagag tgaagcgaca tgagaatatc gtagagtttt tgatagagta caagtgtctg
    18781 aggatcttgc tcagcatttt ccttttcttg ttgaagggcg ctagcgatac gagtcaagac
    18841 atcttttacc tgactgttta cttcatccaa gtctgcatca gccttgtttg tggcagcttt
    18901 tagattttct acttcttctg ccaaggattg tctgattcct tcttcatgga tttgttccaa
    18961 gagttgattt gccttgctca aaagactttc tacttcttcc ttgctatctg tcgcagatta
    19021 ttggttgcta tctaccatgt attcctaaaa caggagagtt ataatccaag attacaaggc
    19081 cttacagaaa taagaaatcc agataagaca atgtccgtcc aagacgctat tcgcttcgca
    19141 cagcagcgcg gattcaatat gctttaattt taaagtttag gtgtcaagac ctctttttag
    19201 tgtgcccaaa atttagagaa gtaatcaatc aactaacttt tatttttttc aaactttcag
    19261 taaactgacc taaagctaac tcaatctgtc tttgttcgat aggcttgtct ttgtagatgc
    19321 ttctgctatc agctagaagt tgatctactt ttgccaagac tgccttctca tcaaaagttc
    19381 caggtggata gttggattgc agggatggaa tcttgttttt caaagccgct tcatatccct
    19441 tagtttgaac cttgatgtag tgattgtggt cgccatgagg aatcacaaaa ccttctgaat
    19501 cttcacttat aattcgattg gcatcaaaac catgaccatc ttcttcctca tggtggacat
    19561 gtagtgacgg attacttaat acagaactag aagaacttcc tacctcttcc gtgttagagt
    19621 gtgatggggg attgttaaga gatgacttag gaatatagtg atagtgatcc ccatgtctta
    19681 ctatataagc atcacctgta tctctgacaa tatcattagg gttaaagaca taaccatcat
    19741 ctactgcaga aacaccatta ttcggtgtca ccggcaaaga ttgactgaga gctgtagtat
    19801 tccctgataa ttatactttt gcgactgcta attcacctgc cgacaagtca ctctcaggaa
    19861 tgaaatgata gtgaccacca tgtggtacta tcgtagattg aaatagaata tgagcaaatt
    19921 gataagggga ttttaaagta atttctaaca atgatttaga aactatgatg tgctattcta
    19981 aattcaactc actatatata accatcatcg gtagtataac gtccctgtaa ttttgctaca
    20041 gatacttctg cactagctcc tttatcgtct ttaccatgtt cttgtttttg gcgattgatt
    20101 tcatcttttg ttcgtacatt ttctgcatga gcttgatctt taaggtaaac ataatacttt
    20161 ccatctccct taataatata tcctccctta acctaactga cgatatcttg atctttcggc
    20221 tgatagttgg gggctttcat tagtagctct tcactaaaga gcgcatcaaa aggaacttta
    20281 ccattatagt agtgataatg atcgccatga gaagttacat aaccttgatc tgtaatctta
    20341 ataacaattt gttttgcttg aattccttct ttttgactaa cctagtctgg agtcaaattt
    20401 tcagtcttct tagtgtcttt attactgttt acatatgaaa cacgattttt atctgtattg
    20461 gcctgttagc tatgttggtt cagagcataa acacacagac ttaaggaaag gataacaaca
    20521 gatccagctg ctatatattt cttttta
//
LOCUS       DAVKLW010000036        19799 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKLW010000036 DAVKLW010000000
VERSION     DAVKLW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.036"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..634
                     /locus_tag="VW324_001335"
                     /pseudo
     CDS             1..634
                     /locus_tag="VW324_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(751..1098)
                     /locus_tag="VW324_001336"
     CDS             complement(751..1098)
                     /locus_tag="VW324_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9726968.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1209..2984)
                     /gene="spxB"
                     /locus_tag="VW324_001337"
     CDS             complement(1209..2984)
                     /gene="spxB"
                     /locus_tag="VW324_001337"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9726969.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3191..5434)
                     /locus_tag="VW324_001338"
     CDS             complement(3191..5434)
                     /locus_tag="VW324_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9726970.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5444..5815)
                     /locus_tag="VW324_001339"
     CDS             complement(5444..5815)
                     /locus_tag="VW324_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9726971.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(5826..6221)
                     /locus_tag="VW324_001340"
     CDS             complement(5826..6221)
                     /locus_tag="VW324_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9726972.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6405..6503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6587..7378
                     /gene="thiD"
                     /locus_tag="VW324_001341"
     CDS             6587..7378
                     /gene="thiD"
                     /locus_tag="VW324_001341"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9726973.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7588..8220)
                     /locus_tag="VW324_001342"
     CDS             complement(7588..8220)
                     /locus_tag="VW324_001342"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9726974.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8213..9016)
                     /locus_tag="VW324_001343"
     CDS             complement(8213..9016)
                     /locus_tag="VW324_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9726975.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9016..9540)
                     /gene="thiW"
                     /locus_tag="VW324_001344"
     CDS             complement(9016..9540)
                     /gene="thiW"
                     /locus_tag="VW324_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9726976.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSATKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9545..10237)
                     /gene="tenA"
                     /locus_tag="VW324_001345"
     CDS             complement(9545..10237)
                     /gene="tenA"
                     /locus_tag="VW324_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9726977.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10248..10898)
                     /locus_tag="VW324_001346"
     CDS             complement(10248..10898)
                     /locus_tag="VW324_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9726978.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10900..12285)
                     /locus_tag="VW324_001347"
     CDS             complement(10900..12285)
                     /locus_tag="VW324_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726979.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12286..12846)
                     /locus_tag="VW324_001348"
     CDS             complement(12286..12846)
                     /locus_tag="VW324_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9726980.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(13109..13197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13406..14035)
                     /locus_tag="VW324_001349"
     CDS             complement(13406..14035)
                     /locus_tag="VW324_001349"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9726981.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(14037..14819)
                     /gene="thiM"
                     /locus_tag="VW324_001350"
     CDS             complement(14037..14819)
                     /gene="thiM"
                     /locus_tag="VW324_001350"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9726982.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(15101..15769)
                     /locus_tag="VW324_001351"
     CDS             complement(15101..15769)
                     /locus_tag="VW324_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9726983.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15984..16072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16324..17460)
                     /gene="lctO"
                     /locus_tag="VW324_001352"
     CDS             complement(16324..17460)
                     /gene="lctO"
                     /locus_tag="VW324_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9726984.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(18085..19575)
                     /gene="lysS"
                     /locus_tag="VW324_001353"
     CDS             complement(18085..19575)
                     /gene="lysS"
                     /locus_tag="VW324_001353"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9726985.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19703..>19799)
                     /locus_tag="VW324_001354"
     CDS             complement(19703..>19799)
                     /locus_tag="VW324_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9726986.1"
                     /translation="YDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         5935 a         4341 c         3674 g         5849 t
ORIGIN      
        1 ccacgtccag aacatcctaa gaaagcagac gctcaaacca ttgtcgcgtc taaaaataaa
       61 atctcaattc aagaagacaa gaaagcgctt taaaaccagt agaccttttc ataaggttcg
      121 cttgatgtcc caagatgagg ctggtttcgg tagaatcagt aaactgggat cttgttgggc
      181 tccaatagga gtaggtccac atgtccatag tcactatata cgagaatttc gctattgtta
      241 tggagctgtt gatgcccata caggcgaatc atttttctta agagctggta gatgtaatac
      301 tgagtggatg aacgcctttt tagaagagct ttcacaagct tatccagatg attatttttt
      361 actcgttatg gacaatgcta tatggcataa atcaagtacc ttaaagattc cgactaatat
      421 tggctttgca tttattcctc catacacacc agagatgaag cccattgaac aagtgtggaa
      481 agagattcgt aaacgtggat ttaagaataa agcctttcaa actttggaag atgtcatgaa
      541 tcaactccaa gatattatac aaggactgga gaaggaggtg ataaagtcca tcgttaatcg
      601 gagatggact agaatgcttt ttgaaagcag atgagtatta tatgcaattt ctttatataa
      661 aaagaccgga ttgctccgat ctttcaatag ttcatattct caatttctat tttaaaaata
      721 gctaaggtta acgtcaaatg actacgcgac ctatttcata cgataaaaat caagcactag
      781 accagcaggt ccttgaacta ataaggactc tgttccccaa tcggttacag ttggtccgtg
      841 taaaaccttt ataccaagct cgttcaaccg tttgtagttc tggtctacat cctcaacctc
      901 gatatgaata atgattcctg actgaaagtt ttccaaagga accaaatgat tttgtgacaa
      961 cataaggcag tgactaccaa tcgtaaactg agcaaaacca tcatcagcat aatctgcctt
     1021 tttatccaag atatgctcca agtcagcaca gacttgggga acatttgaaa cgataatatc
     1081 taattgattt aaattcattt actctcctcc ataaaaagac cggattgctc cgatctttta
     1141 aagttctgct ctatgaaaat caaagaataa agtctacaag tttcatattt gattttcggc
     1201 gagaggaatt atttaattgc gcgtgattgc aatccttctt cttccaagaa gagacggaat
     1261 ggtacgagtt cttctgcttc gtatttttcc ttgaaggctt tgatagcttc ttctgagtga
     1321 agttttggat ccaattcaag tacttctact ggaagtggac ggtgttgagt gatgcgagca
     1381 tcgatgacaa cagttttacc ttctttgttc aatttaacag cttctgcaac aactgcatcg
     1441 atgtcttcga tacggtcaac tgtgaatcca acagctcctt gagcttccgc aattttagcg
     1501 tagtcagcgt ttgtgaagtc tacaccaaac aagtgtttgt ttgtatcttc gtatttgttc
     1561 ttgatgaagc cgtactcagc atttgagaag acaaggttga taactggaag gtcgtattga
     1621 acgtttgtga taacgtctgg gtagcacatg ttgaatgctc cgtcacccat gatgttccat
     1681 acttggcgat ctggattgtc tttcttagca gcgataccac caggaagggc aatacccatt
     1741 gtcgcaaaga gtggagatgt acgccacatg ttcttaggtg tcatgtgaag gtgacgagta
     1801 gatgtttgag tagtgttacc tacgtcgatt gagtagatag cgtcttgatc agcatgtttg
     1861 ttgattgcat tgtaaacttg atacaattgc aattcaccct cagttttacc ttcgagtttg
     1921 ttcatgtaat cacgccagtt ttggttgttc ttaacgtttg cacgccacca tggagttgat
     1981 tcaactgggt ttactttgtc aaggatagct ttagctgctt gaccagcatc accaaggatt
     2041 gaagcgtcaa gggcatgacg tttaccaagt ttgtaagggt cgatatcgac ttggatgaat
     2101 ttttcagtgt tcttgaatgc ttcgtaaact tcagcaaatg ggaagtttga accaaggaaa
     2161 agaactgtgt ctgcttcaaa gaccacttcg ttggctggtt tccaaccaac acggtaagca
     2221 gaacctgtca aaccttcata gttccattcg aaagcttcaa agtttttacc agttgtgatg
     2281 attggtgctt tgattttacg tgacaattca gtaatcactt caccagcttt aacaccacca
     2341 aatccagcat agataactgg gcgttcagca ttgttcaaga tttcaacagc tttgtcgatt
     2401 tcaacttcgt tcaaagcagg agcgatgaat gagcgttcgt atgaacctga accgtagtat
     2461 gagttttcat cgatttcttg gaaaccgaag tttactggaa tttcaacaac agctggacct
     2521 tttttagaaa ctgcagcacg gcaggcttcg tcaattactt ttggcaattg ctcagcgtaa
     2581 gctacacgtt tgttgtaaac agcgataccg ttgtacattg ggttttgatt aagctcttgg
     2641 aaagcatcca tgttcaattc gttaactgga cgtgatccaa ggatcgctag gaatggagtg
     2701 ttatccatag ctgcatcgta aacaccgtta atcaaatgag tcgcacctgg accacctgaa
     2761 ccaactgcaa ccccgattga gccgccgaat ttagcttgca taaccgctgc aagagcacct
     2821 gtctcttcgt ggcgaacttg taagaagcgg atatctttgt cttcagccaa agcgtccatc
     2881 aatgagctga gtgttcctga tgggataccg tagattgtat ctacgcccca tgttttcaat
     2941 acgttaagca ttgctgcaga tgcagtaatt ttcccttgag tcataatgat aactctcctt
     3001 caattttttt agacttggag aatacgatta catagaattg gaaacgttct ccaaattttt
     3061 actattccac tgtatcatat ttatgctgac ttttctaaaa atctgctcaa aactctctat
     3121 tctctattct aatacagttt tgaaagtgtc gtcatttctg ttttataaca aaaaaatcta
     3181 gtcattactt ttagtctatt ttactaaaat ttaacagaag ggaactggtc agaacagata
     3241 cagaactaaa ggccatggct agacctgcca attctgggtt gagagccagt ccaacacctg
     3301 aaaagactcc tgctgcaatc ggaattccga caacattgta gataaaagcc cagaaaagat
     3361 tgagcagaat acgatgaaag gttttcttac tcatgtcgaa ggcacgcacc actcctaaaa
     3421 gattattggt tgtcaacacc aaatctgctg actcgatggc gatatctgtt ccagctccca
     3481 tagcaatccc cacatctgct acactaaggg caggagcgtc attgatccca tcaccaacaa
     3541 aggctacttt acctgactgt tgcagtttat ggatttcatg ggctttttct tctggcaaga
     3601 cgcctgcaat gacctcttca attccgattt gatctgcaat agcacgcgcc acaccagcat
     3661 tgtctcctgt cagcatgact gtcccgagac cacgtttttt tagctgactg atggctagct
     3721 tagcattttt cttaggaata tcttgcaaag caagcaagcc tttgatttca ttgtcaacag
     3781 ctaagaacac aactgtctta gcttcttttt ctagttcttc tagtttatct tgataagtat
     3841 tagaaatatc catgccatcc agcattttag catttccaag taaaacttgt tttccattga
     3901 ttcgccctga aacacctttc ccgtgcaagg cctgaaaatt ttcaacagtt tgaaactcaa
     3961 gtccagcttc actcgctcgc ttaacgatag cctcagccag tgggtgttga gaagcatctt
     4021 ccaaggaggc tgccaatcca aacacttcta cttcgtcgcc gatgacatct gttaccacag
     4081 gtttcccttc cgtcaaagtc ccggtcttat caaagacaag ggtttgaact ttctggattt
     4141 cctgtaagac agttccattt ttgaggagaa cccccatctt ggcactacgt cctgtcccca
     4201 ccataagggc tgtcggtgtt gcaagtccca aggcacaagg acaggcgata atcaaaaccg
     4261 ccactccgta gagaagagag gacacaaagc tagctccaag cacaaccaca ctatccctga
     4321 gcaagacgaa ccaaacccaa aaggtcatga ttcctaaaat gacaactact gggacaaaaa
     4381 tccctgaaat cttatccgtc aagtcctgaa tcggcgcacg acttgtctga gctttcttca
     4441 caaaatccac aatctgagcc aaaacagtct ctgagccaac tttttctgct ctaaagacaa
     4501 gcgttccact atgattgatg gttgagccaa tgacagtatc tccaactgtc ttgtccacag
     4561 gcagactctc acctgtcacc atggattcgt caatactaga gacaccttct actacgacac
     4621 catcaacagc aatcttttca ccgggacgca ctcgaatcag gtcgcctacc ttgacttgtt
     4681 ccaaaggaac ttggacataa ctatcatcac tcaagacttc tgcggtttta gcttgcaagt
     4741 ccagtaattt ctccacagct tgggacgtat tttttctcat tttttcctca aaaactgctc
     4801 ccaaaagaac gaaaaagagg ataaatccag cactttcgaa gtaaacaggg agaccagcaa
     4861 agagagcaac taggctatag aaataagcca ctagagttcc cagcgcaacc aaggtatcca
     4921 tgttggcatt gtgcttttta aaactggccc aagcactctg gatatatggc ttacctgcaa
     4981 ctaacataat aggcgttgtt gctagaaagg ttccccaatg catgacttga tgactaatgc
     5041 tacctgccaa catcccaatc atgagaatca caagaggcac agtacagata ctagtaatcc
     5101 aaaaacgttg caggagagat agagattttc gagtcttctc aacgactgta tagcttccct
     5161 tttgcatctt catgccacaa gaaaattcat gtcgccctaa ttcttgaggt gtaaaacgaa
     5221 tgactttctc ctcatctacg ccgattggtt ccaagatacc ttcttcttca aacagaattt
     5281 ccttataaca gtttgaagga gtagcacgat gaaaggtaat ctcagctgga attccctttt
     5341 gaagctggat atgggctgga tgatagcctt tttcagcttg gatacggatt ttttgaatgc
     5401 cattttctaa gcttgctttc acaatttctg tcatagtctc cacctactct acaatcatct
     5461 tgccgtgcat catgttcata ccacaagcaa agctaaactc tccagcctgt tcaggcgtga
     5521 tttccactac atactcttcc cccattggca ggttcgcatg tacaccaaaa tctggaaaaa
     5581 caatttgatc cagacatggt gaaggatcct tgcggtcaaa gacaatgcgg gctggcactg
     5641 atttcttgag gacaatcaac tcaggagtat agcctcccat gacttccact cgaatctctt
     5701 ggtatccgtt tttttgctgg gctttttgtc cagatttttc aggctttttg aaaaaccaaa
     5761 acaagataaa cgcgataagg gcaatacaaa taatggttac aatactattt aacatgacgt
     5821 ctcctttaca tacaattaca tcttacttct gttacagcac ttgatttctt ctctgaaatc
     5881 acagcttcca agtcttccaa gtcagtctga gtaaattcac attctacaat caagtcagcc
     5941 aacaaattcc taatcctacg ggaacaaacc ttgtctttga tatcttggac aagtaaatcc
     6001 cgactttggt ctagagttaa aagcgctgaa taaacaaagg acttgccttc ttttttccga
     6061 gtcaaacact ctttctcaac tagacgagcc aaaagtgtct gaaccgtgga cttggaccag
     6121 tcaaaccgct ctgccaaaac cctaatcaaa tctgtactgg tctgctcccc ctgcatccaa
     6181 ataatcttca tgacctgcca ttctgcatct gaaatctgca ttaccatacc tccaaaatct
     6241 acatttgtca attacactca acagtatact cccaaaatct acatttgtca attatagaaa
     6301 taatattttc ttcgaaaaat agaattttaa tcatttgaaa aacgatttgc agtcaaatat
     6361 tactatataa acaataaaaa tatgctatac taaagaaaaa agaaaacaac cactaggggt
     6421 gcgtaaagct gagattaacg actgttagat ccctctgact caatctaggt aatgctagct
     6481 gatggaagtg gaaatgataa tggggactag cagtcttcta ttgcctttct aaaacagact
     6541 agcttgttct taagaataca aacttcagtt ggttgggagg ttttagatga cttatttacc
     6601 cgttgctttg accattgcag ggactgaccc tagtggtggt gctggcatta tggcagattt
     6661 aaagtcattc caagcgagag atgtctatgg aatggctgtt gtaaccagtc ttgtcgctca
     6721 aaataccaga ggtgttcagc taatcgagca cgtttctcct caaatgttga aagcccaatt
     6781 ggagagtgtc ttttctgata ttccacctca ggctgtaaaa actggaatgt tggctactac
     6841 tgaaatcatg gaaatcatcc aaccctatct taaaaaactg gattgtccct atgtccttga
     6901 tcctgttatg gttgctacaa gtggagatgc cttgattgac tcaagtgcta gagactatct
     6961 caaaacaaac ttactacctc tagcaactat tattacgcca aatcttcctg aagcagaaga
     7021 gattgttggt ttttcaatcc atgaccccga agacatgcag cgtgctggtc gcctgatttt
     7081 aaaagaattt ggtcctcagt ctgtggttat caaaggcgga catctcaaag gtggtgctaa
     7141 agatttcctc tttaccaaga atgaacaatt tgtctgggaa agcccacgaa ttcaaacctg
     7201 tcacacccat ggtactggat gtacctttgc tgcagtgatt actgctgaac tagccaaggg
     7261 caagagtctt taccaggcag ttgataaggc caaggccttt atcacaaaag ctattcaaga
     7321 tgcccctcaa ctcggtcatg gttctggtcc agtcaaccat acaactttta aagattaaga
     7381 aaaaaaactc tctagttccc actttaaggg aattagagag tttttatact cttcgaaaat
     7441 ctcttcaaac tacgtcagct tccatctgca acttcaaaac actgttttga gctgacttcg
     7501 tcagtcttat ctaaaacctc aaggcagtac tttgagcaac ctgcgactag ctttctagtt
     7561 tactctttga ttttcattga gtattaatta ggaaagaatg tcatgcaact ttgttaaaaa
     7621 ggcttgcgtt tttgcctcaa tatcttctgc ttgcatcaaa tcacgtacaa cagctacacc
     7681 agctatgcca gtgcccataa gctgatcaat attctccgaa gtcaagccac caatagcaac
     7741 tattggaatg gcaaccgttt ggcaaattgt tttcaaggtt gaaatcaggg tgatgggtgc
     7801 attttccttg gtggttgtcg gaaaaatggc tcctgtcccc aagtaatctg cacctgattt
     7861 ctccgcttcc agagctcttt taaccgtttt agcggtgaca ccgaggattt tttcaggacc
     7921 caagacttgt cgggcaaccg aaactgggag ttcgtcatct ccaatatgca gaccagcagc
     7981 atcaaccgca agacaaacat ccaaccgatc atcgattatc aagggtacct gataagcatc
     8041 tgttatttcc ttgacttgtt ttgccagttg ataatattga ttggttgtga gatttttttc
     8101 tcgcaattgg actatggtaa cccctgaacg gcaggccgtc tcaacttttg caagaaagct
     8161 ttccacggaa tcttgatagc gattggttac cagatatagt ctaagtgctt ctctattcat
     8221 aaacctctcc tttgatggta tctagccaat tttcatctct tcttaggagc gaaagctgat
     8281 tgagtacttg gtaacgaaat tcttccaatc ccattccttg aacaactatt ttctcagcag
     8341 cgatattgag ataagagact gctaagcaag aagcttcaaa accagtcttt ccttggctga
     8401 gaaaaacggc tgttaaggct ccaaccaaat ctcctgtccc tgttatccag tctaattctg
     8461 cacagccatt tcccagtaca gcgacctgat ttttcgaaac gacgaggtcc ttgggacctg
     8521 tgactaagaa agacatacca tgataggtct gacaccagtc tttcaagact tgaagcaaat
     8581 cctccgtttc ttgatcttta gcactcgcat cgaccccaac gccgtggtgc tttaatccaa
     8641 caagacttcg aatttctgac atgtttcctt taaggaccgt aggtctatag tctaaaaggt
     8701 ctctgactaa gctcttacga atggatgaag ctgttacgcc aaccgcatct actaccattg
     8761 ggagacaaac ttgagctgca taagaagctg ctatacggat tgctttctcc ttctcagctg
     8821 acaaatgccc caaattgatg aagagagcct gactttgctt agtaaaatca agaacctcac
     8881 ggggatcatc tgccatgaca ggtttgcatc ccagagccaa aatcccattt gccagcatct
     8941 cacaagaaat ctcattggtc atacagtgaa tgagggaact agagcctata ggaaagggat
     9001 ttgtaaattc ctgcatcagt ctatcctttc actaaagaaa tatccctgca cttttttaaa
     9061 gaattcctgc ttgattaaaa atcgaaaggc aataaaggaa atcgctgtac caatcaaggt
     9121 tgctccgaaa aatcgaggcg tgtagataaa ccagctaagc ttagtagctg atcctgtaaa
     9181 gagtaccata acaggatagg aaacaataga accaataata cctgttccca caatttctcc
     9241 caaggcagaa aagtaaaatt ttcgaccgta cttataaaag agacctgcta gaagggctcc
     9301 aaaagtcgct cctgtgagag ctaaaggcgg aatcccttga gtcgtcatac ggataaaggc
     9361 tgtcactgta gccatagcca aggcataaac aggtcccatc atgattcccg ctagaatatt
     9421 gactacactg gacatcggtg ccattccctc aatccgaaag ataggtgtaa ggactacatc
     9481 aagggcaatc atcatagata aaatggtcaa tttgtgaact tgtagttggt gctttctcat
     9541 gtttctattc ttctcctttt tctaaagact gtaaatcgct cttccatgtc tggtgttggt
     9601 aagccatctc ccaaaacttg gcttccatat gaacactgat gtggaaggca tctagcattt
     9661 tttgcttatc tgtctcatca ctttctcgat agagctgatt gaccagtgct ccctcctctc
     9721 tgatctgttg ctctaactca tccgtaatat aagtttcaat ccattgttga tagagaggat
     9781 ttggtgatgg tttaagatta agtgatttgc ctatatcatg gtataaccaa ggacaaggaa
     9841 gcaagcttgc aaaagcgatg gctaagttcg gttctgcaaa ttgcctataa atatgagaaa
     9901 tgtaatgata acaggttgga gcgattggat gttgctccat ttcctggtcg ctgatttcca
     9961 attccttgaa aaattgttgg cgaataaata actcaccctc cactaaaccc tgagcatttt
    10021 gtttcaagag tcttttcatc tcttggtttg aagtcttatc agccaaaaga tgatagattt
    10081 ctgagaaagc cttcagatag taggcatcct gaatcaggta atagcggaaa atggcaggtt
    10141 ctaaattccc ctcttgtaat tgtaaaataa agggatgatg aaaggaagcc tgccaagctt
    10201 tcttggataa ttccatcgca atatctgtaa attccataat aactccttta taaaaataga
    10261 ctggtttgaa gcaataaaaa gaaaagcagg tagattaatt ttgttttttt aggaatataa
    10321 aaagtccgat agctattctt caactgtgca tgttcgtcat atccgtgagc agatagagct
    10381 ctcaggtaaa gatggcgcca cctaaagact gtcatcagaa ccttactgta aatcaagggc
    10441 gaccaaaaat gtagttcttg accacgtaat aggcaagctt ctttgaggga cttgatttct
    10501 tgctgaatga gaggaaagga attgaatacc acaatcaagg cataggacca agagcgtgat
    10561 agcccctttt gagccaagta caagagaagc tcttttagtg aaatagagga aacaaagaca
    10621 aggccgatac aaactgtcac aaaggccctc gttccaagca tgactgcctg tgaagcatct
    10681 ccgtgtaact gaactgccca gtagttggca aaagatggta aaatggcaag tatgatcatc
    10741 caagctaaca ttttaaatcg acggtaatag agcataaaga gaatacaaaa tgcgactacc
    10801 gaaagattca gagcaatcga aggaatgaaa gatgtttcca aggataaaat cagcaagaag
    10861 agactgataa tcggtgtctg ggttgctact ttgaccatac tatctcacct ccccttgggt
    10921 attgctactc tgagatgtaa gtggtttggt aatggtcact tctttcacat gccgaagacc
    10981 ctgactagtc atctcaatcc aataatcaac cacagaaatc aaagggtcta aacgatgact
    11041 aatgagcaga aaacttcttc cttgattcct ctcctccaca atccacttgc aaaaataatg
    11101 gcaggctcta tcatccaaac ctgcaaaagg ttcatctagc aagatcacgg aagccttact
    11161 ggtcaagatg gtcaagagct gaagaatttt ttgctgacca ccacttaatt gatagggact
    11221 cttatcgact gcctgctcca aatcaaaata tcgtaaagct tgaaaaatcc gctgatttct
    11281 ttcagaatca ggtccatcta attgaagctc ctctcgcaga ctgactcgga taaactgctt
    11341 ctcagcttcc tgaacaatac ccgtcagatc acggtacaaa ctctttttct ttttcaggac
    11401 tgtccccttc catgcaatgc gccccttata cttttgaaat tgaagaatag accgaaagag
    11461 ggttgatttc ccgacaccat tgtcacccag gatacaggaa atcccttgat agaatgtgaa
    11521 atcagcaatt gaaaagaggg ggcgattacc aagctcacca gtcacacggt tcatatggaa
    11581 taattccggg ctagaagcaa cttcctttga agcaacctgt gtcatctcat aggaagggat
    11641 ttgaaacact tcccttagtt ttccgtctct tagctcaacc atatggtcga tataggcttt
    11701 atagtcagat aaatcatggt cgcacaaaat aactgtcttc ccatcaagag ccaactcttt
    11761 tagaatctcc aatatctcga ttctgctctt gcggtcaatg gaagcgaagg gctcatccaa
    11821 gagatagacc ctaggattca tggcaaagag gacagccagc gctgcttttt gcttttcccc
    11881 acctgatagg tgatgaatgg gacggtgtaa gattgcctcg caacgacatt gctggacaac
    11941 ctctgctatt ttagaatcaa tttcctgaag gtgatagccg atattctcca aggtgaaaat
    12001 cagctcctca aacaagttct ccatggtaaa ttgatgattg ggattttgaa agagaatgcc
    12061 aaccttctgg acacgttcga cgatagaaag ctgactgacc tcactcccat ctatcaggac
    12121 ttgaccgcta tagggaagag aactgacttg ggcaatcatt tgaaagaggc tggattttcc
    12181 tgaaccactg ctcccaacta acaaggtaaa ggcttgcgca tgaaaagtaa aatcaaacgg
    12241 ctcagagaaa attggggact gaatcgctcg tagttccaga cccatctatg cttttcctcc
    12301 agttgcaaac tgatgataga gtttgacaat ggcacgaacc aagatggtac agaagaaata
    12361 aacagaaata aaacgtacca caagcaagga aaggacaaac ggaagggaaa aggcgtagta
    12421 acctaactta atgtattcat agacaaagct aacaagcgta atcccaatac tattagcagt
    12481 tagagagagc caactttcat agcgattctt agttacgata aaaccaaatt cacttcccaa
    12541 accttgaacc aagccagaca aaagagctcc tagaccaaat tggctaccat aaaggacttc
    12601 agcaagcgca gctagcactt ctccaatcgt tgcacttccg actctcggaa caaagatggc
    12661 agcaatgggc gcagccatac accagagacc gaagaggatt tcattggcaa aggcctgcaa
    12721 accaagaggt gttaagagta gactgagaat attatacaca tatcctgaac caacgaaaac
    12781 cccaccaaaa aagatagaca agaaagcaag caagataaca tcttttaact gccatttttt
    12841 caacataaaa aaactccttt ttttaaagaa aagtggggca ctcaagaaga ccgacctaaa
    12901 tactttgtat agcagactga atttagaaca gtacacaaga acactaaaat atttctagaa
    12961 attaatttga attttctaat tgatttgttc gcatcttatt tcaatctact atatcatctt
    13021 catccagttt cgtaaaagaa aaaactctaa ttacagatac gaattagagt tcagcttaca
    13081 agattagaca gttcttttcg acatacgaaa aaaacatttc acatttccct tcgccagtct
    13141 taactgtatc aggttcaatg ggtatcatct cagcctaaag caccccaaat gtctttatta
    13201 tttaattatg tgattattat aacacacatt ttatactagt tcaagaaatt gaactggaaa
    13261 tacagccttg cactcacaaa gacagcagat ctttcttttg caaaaaacaa atgacctgtt
    13321 tgatgaatta gccattcaag ctgaatctgg acatagcttt ttaaaaaagg aaaatcctac
    13381 ttacttagaa tccaaggata gatatctatt gttcactcat ttcccgaaca gttttttcta
    13441 tattttttgc atacgatatt gccgaaatga ttgaaacgcc atccatattg gtctttataa
    13501 tgtctttaat atgtttcgtc tgtatcccac caattgcaac taaaggcatt tgtggcaata
    13561 gttttctcat caattcaaga ccttcataac ctatagcacc accagcatca tcttttgact
    13621 gggtatcaaa tacaggccca acacctacat aatctacata ttcaactttt gattgttgaa
    13681 attcttcttc gtttcttata gaaagaccaa ttattttatc tggcatcaat tttctaattt
    13741 catcaacacc aatatcatct tgacctacat gtacgccatc ggcgtcaatt tccattgcta
    13801 aatctatatc gtcattaacg ataaatggaa cattgtattt tttacaaagt tctttaattt
    13861 ggatagctag ctcaagtttt tctaagcctt ctaaagcacc ctcacctttt tctcgaaatt
    13921 gaaataaggt tataccacct tttaaggctt cctcaacgac tgtatataga ttttttcctt
    13981 ggcaagtagt cgttccacaa ataaaatata gttttagtaa ttctttatga aacatcttac
    14041 ttcactcttt tgaattcctt tacatcttca tctgtaatct cgtataaggc atttataaat
    14101 tcaactttaa atgtcccagg aagatgtcca tttggacgtt tttctgccat ttctccagcg
    14161 atattgtaaa ctaacattgc tgtttctaat gattttaatt cttgaccttt ttccagtccg
    14221 ataaaacttg ctactacagc tcctaataag catcctgtcc caatgacttt cggcatcata
    14281 gcactaccat tatgaatcgt taccacttct ccattaacag caatggcatc cacttcacct
    14341 gttactacta ttggaatatt gaacttctca tttgctgcta gagcaatttc gtcaatatta
    14401 tctacgcccg cactatctac tcctttagat gccacatcta ttcctactaa agaggcaatc
    14461 tctccagcat tccccctaat cgcagctagt ttgtaattgt tgattaaatc atctgctact
    14521 ttttttctat attctcctgc tccacaggct acaggatcta aaactactgg gacattatat
    14581 ttctctgcaa ttttcagagc agcttggtat aatttccaat tttcatctgt caatgttcct
    14641 atgtttatta ataaaccacc agcatacttt aacaaatcct ctaaatctgc tggaaactca
    14701 ctcatggctg gtgaggcgcc cagtgctact aatccatttg ctgtgaaatt ttttactaca
    14761 tcattggtta tacaaatgac caatggtgct ttttctttta ataattttaa acttgtcata
    14821 ttgaaatcct tccttttcac tttatacgat ctactaattt cgatttatct ttagttgaga
    14881 atttttttca tttacattga atgatttata ctcaatgaaa atcaaagagc aaactaggag
    14941 gctaaccgca ggttgctcaa aacactgctt tgagcctgta gataagactg acgaagtcag
    15001 ctcaaaacac tgttttgagg ttgtggatag aactgacgtg gtttgaagag attttcgaag
    15061 agtcttacct catcaaattt gtaaatatca tgagccttct ctagacatcg taaccaatat
    15121 caaaaaaagc taattctaaa gcgactgctt gattccagcg ttgctgaagt tctgtcaaat
    15181 cttctcgatt tttaccgaca cgattgagtt catcaaccag aaattgaacc cactctgcaa
    15241 agaaaggacc tctgtggaga ttgatccatt ccgaatgaat atagacttca ggtagagcca
    15301 aatctttaga accccagtct aaatagagac cttctgcaat gaccagcata accaaaagat
    15361 gggcatagtc tgatgaagcc actgcccaat acattagatc ctgaaaggct tttgttacag
    15421 ggtgcaaagt cacttctaga tagtcattct ctgctacttt taactcttta aaagcctttt
    15481 ggaaataacc atcttcatct gcttcaagaa agcctagttg cttggcaaaa cgaagcttgg
    15541 attcaagttt atctgcgtga gctacgcagg cacccagcat ggataagaag gcatcaaaga
    15601 agtgataatc ttgaatcaga tagtccttta agaccttatt ctcaattgtc cccgcaaaaa
    15661 gttccttaac aaaacgatga ttgattgcag cctgccaatc cttctgactg ctttttaata
    15721 attctccaac agtcaaacct ggctgaaatg catagtcttg tgtttccata tttacttctc
    15781 ctctctttac ttgttagtaa ttaataaaac accaagaaat atcaagcaaa atcgtaattc
    15841 cacttgatcc ttttaaagca catcgagagc atttgcagag agctaactaa acaagcctat
    15901 ccagtttata taaacaaaaa actccaatta caatcaagaa ttagagttga cttacaagat
    15961 tagaccgttc atttcgccat acgaaaaaac gtttcacatt tcccttcgcc agtcttaact
    16021 gtatcaggtt caatgggtat tatctcagcc taaagcaccc caaatgtctc tattatttaa
    16081 ttactgaacc agtatagcaa aaaatgaaag ccctagcaag atatttgacc gaaaaatatc
    16141 tttatatagt agattgaaac tagaatagta cacctctact tctaaaacat tgttagaaat
    16201 cgatttgact gtcctgattg atttgtccta ttcttatttc attttactat agttttcgat
    16261 agcaatttat tcttccaata cacgaagaaa aacctccaca ttcagtggag gcaatctgtt
    16321 ttatcaatac aattttaagt cacgagggtc aactgggaag gttgggttgt atggattgtg
    16381 acggagcttg aagtgtttga catcttcaat ggtctgagct ccagacaatt gcataactgt
    16441 cttcaattcc gcattcaagt gttcaaagac ttgacgcaca ccgacactac caccgagagc
    16501 caaaccatag atgacagggc gtccaatagc aaccaagtct gctcctgatg ccaaggcttt
    16561 aaagacgtgt tgaccacgac gaacaccaga gtcaaagaca atcggcacac gtctatcaac
    16621 tgcttctgcc acttcttgaa gcgagtcaaa ggcagctggt ccaccgtcga tttgacgacc
    16681 accgtggttg gttacccaga taccagaagc tcctgcagca agcgaacgtt caacgtcctc
    16741 acggcattgt ggtcccttga catacacagg aagaccagag tattcagcga taaattctac
    16801 atcgcgtgga gacaagcgtt gtttagctga tttgtaaaca aagtccattg atttaccagc
    16861 accttctggc aggtattctt caacaatcgg catgccaact gggaagacaa aaccattacg
    16921 cttatccact tcacgattcc cccctacagt agcatctgcc gtcaagacaa tcgctttata
    16981 accttcagcc ttcacacggt ccatgatgtg gcggttgata ccgtcatcct tactaaagta
    17041 aaattgaaac caatgaggtg tcccttgaag ggcttcagaa atctctggaa ggtcaacagt
    17101 agagtaagaa ctggttgtat aaagagaacc aaactcatgc acaccacgcg cagtcgccac
    17161 ttccccctgt tcatttgcca atttatgagc cgcaacaggc gccataatga ttggtgaaga
    17221 tagtttttca cctgcaaatt caatctctgt acttggattt tcaacatcgc aaagtgtatg
    17281 aggaacgatg agtttgtggt taaaggcgcg gatattctct cttaaagtga aagtatcttc
    17341 cgccccacta gcgatatagc caaatgctgc tttaggaata acttgttgcg ccattggctc
    17401 caaatcatag gtattgatga agtctacatg accttctgca ttgcttgttt tgtatgacat
    17461 aaaatgccct ccttgattaa gtaagcgttt gctttttact acacaactat aataactctt
    17521 ttttcaatca ttttcaagag ttttgcttaa aaaccatctt attttttcat acaatatact
    17581 agccctactt tccttaaact ctaaacttta gtctatatta agtgaatcgt tgagattttt
    17641 tgctgccagg catttgaact acttttttat ttgctgattc tatctactaa tactataaaa
    17701 aagcaagtga ataagacacc agaacatcct ctaataggac ctatggactt atatcagtcc
    17761 catatatact gtttaagcaa gatactatcc tagttgacgc taacttccta tcccacttat
    17821 taatttttta tttatagtga atctgaacaa atcaattggg gaatccaagt caatttctag
    17881 tactttttga acagtttcag cgtactatcg taaaatgaaa taaaacatgc acaaattgat
    17941 tagggaattt aaaccaattt ctaacaatgt cttagaaatc aaagtgtact atttcaactt
    18001 caatatacta cacttcattt tctccttaaa ttcagtctct ttttgaatca aaaaatcctc
    18061 tggtaagagc cagaggataa gaatttattt cattgttggg aagagcaata catcacggat
    18121 agttgttgta tcagtgagga gcatgcagag acggtcgata ccgattccca aaccacctgt
    18181 tggtggcata ccgtattcaa gagcctcgat gtagtcatag tcgatgcctg tcgcttcatc
    18241 atcaccaagt tctttagctt tagcttgggc ttcaaaacgg ctaagttggt cgattgggtc
    18301 gttcaactca gtaaaggcat taccgtactc cttagtcatg ataaagagct cgaaacggtc
    18361 agtaaagcgt tgatcttcag gatttttctt agcgagtgga gatacagcta ctggatgtcc
    18421 atagacaaag gttggttgga ttaaagtttc ttcaacaaac tcttcaaaga aggcattgat
    18481 gatgtgacca acctcagtgt agtgtttctc aactggaact ttcttctcag cagcgatagc
    18541 tttagcttct tctaaagtca tgtcttgcca gaaatcgaca ccagtaattt ctctgatagc
    18601 atccaccata tgaacacgct taaatggttc gttaatcttg atttcagtac cttggtagtt
    18661 gactgggcca tcgcccttaa ctgatttagc agcgtgttgg ataatgcctt ccgtcaagtc
    18721 catgatgtct tggaagtctg cataagcttg gtaaacttcg atagaagtaa actcagggtt
    18781 atgagtagcg tccattcctt cgttacggaa gatacggcca atttcataga cacgttccat
    18841 accacccacg ataaggcgtt ttaagtgaag ctcagtcgcg atacgaagca ccatgtcaat
    18901 gttttgggca ttgtggtggg tgataaatgg acgggcagca gcaccaccgg cttcattatg
    18961 aagaacaggt gtttccactt caaggaatcc tttttggtca aggtaacgac ggatttcaga
    19021 gatgattttt gaacgagtga caaagcgttc aaagctttca cggttagaaa tcaagtcaag
    19081 gtaacgttta cggtaaattg tttcaacgtc tgtcaaacca tggaatttct caggaagagg
    19141 acgaagagcc ttagacaagt gtgtgatgtg ggttgccttg atagagagtt ctcccatatc
    19201 cgtacgcatc acttcacctt cgacaccaag gaagtcacca aggtctgctt ttttgaagat
    19261 ttcgtagttt tcttcaccga cagcatcctt acgaacgtag atctgaatct ggccttcgcg
    19321 gtcttgaagg tgggcaaaac caacttttcc tttaccacgt ttggttacca agcgtcctgc
    19381 gatagtagct gtttcgtttt tatcgtgtaa ttgttcttta tcgaggttgg tatatttatc
    19441 ttttaattct tgtgaatttg cagtacgttc aaaacgtttt ccgaaaggat cgattccttg
    19501 ttcgcggagc gcagccattt tttcacggcg aacgatctgc tggtcattta gttcttccat
    19561 atgttctgta gacatgggat cctcctagtt ttactcaaaa ttgaatacga tgagtcattt
    19621 tttgcgatac tcccatttta tcataaataa gcaagaaatt cacggataat ctttaaaaac
    19681 taaaaagaac ctgacgctaa aatcactgga aagaagtgaa agtatctccc gccccactag
    19741 cgatatagcc aaatgctgct ttaggaataa cttgttgcgc cattggctcc aaatcatag
//
LOCUS       DAVKLW010000037        19592 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKLW010000037 DAVKLW010000000
VERSION     DAVKLW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.037"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            98..436
                     /locus_tag="VW324_001355"
     CDS             98..436
                     /locus_tag="VW324_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726987.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            502..2484
                     /locus_tag="VW324_001356"
     CDS             502..2484
                     /locus_tag="VW324_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726988.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYVQLFITLKQNEYVKKIILGLSKTYIAISSLKYFMMTITMVILFTFLMTGQIE
                     LLYIGAASLLVLMLSIIMSFRKLSESYTKILKGDES"
     gene            2481..3104
                     /locus_tag="VW324_001357"
     CDS             2481..3104
                     /locus_tag="VW324_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9726989.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            3101..3601
                     /locus_tag="VW324_001358"
     CDS             3101..3601
                     /locus_tag="VW324_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726990.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKELYKKIYES
                     FKEKNK"
     gene            complement(3767..5113)
                     /gene="gor"
                     /locus_tag="VW324_001359"
     CDS             complement(3767..5113)
                     /gene="gor"
                     /locus_tag="VW324_001359"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9726991.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRSFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            5378..6577
                     /locus_tag="VW324_001360"
     CDS             5378..6577
                     /locus_tag="VW324_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9726992.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            6561..7262
                     /locus_tag="VW324_001361"
     CDS             6561..7262
                     /locus_tag="VW324_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9726993.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            7264..8523
                     /locus_tag="VW324_001362"
     CDS             7264..8523
                     /locus_tag="VW324_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9726994.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            8647..10644
                     /gene="metG"
                     /locus_tag="VW324_001363"
     CDS             8647..10644
                     /gene="metG"
                     /locus_tag="VW324_001363"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9726995.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            10688..10768
                     /locus_tag="VW324_001364"
                     /pseudo
     CDS             10688..10768
                     /locus_tag="VW324_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11065..11385
                     /locus_tag="VW324_001365"
     CDS             11065..11385
                     /locus_tag="VW324_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9726996.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            11389..12204
                     /locus_tag="VW324_001366"
     CDS             11389..12204
                     /locus_tag="VW324_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9726997.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            12279..13205
                     /locus_tag="VW324_001367"
     CDS             12279..13205
                     /locus_tag="VW324_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9726998.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            13271..13537
                     /locus_tag="VW324_001368"
     CDS             13271..13537
                     /locus_tag="VW324_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9726999.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            13530..14228
                     /locus_tag="VW324_001369"
     CDS             13530..14228
                     /locus_tag="VW324_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9727000.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            14239..14718
                     /locus_tag="VW324_001370"
     CDS             14239..14718
                     /locus_tag="VW324_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9727001.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            14726..15454
                     /locus_tag="VW324_001371"
     CDS             14726..15454
                     /locus_tag="VW324_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9727002.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            15496..16365
                     /locus_tag="VW324_001372"
     CDS             15496..16365
                     /locus_tag="VW324_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9727003.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            16340..16780
                     /locus_tag="VW324_001373"
     CDS             16340..16780
                     /locus_tag="VW324_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9727004.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            16903..19449
                     /locus_tag="VW324_001374"
     CDS             16903..19449
                     /locus_tag="VW324_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9727005.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
BASE COUNT         6113 a         3322 c         4238 g         5919 t
ORIGIN      
        1 cctaaatata atcaattcat attaaagtaa tatggaggga ctaaaaagag actattattt
       61 aatcttcatc aaatatttaa attcaaggag aagtaaaatg aaaaaaagaa ttaataagaa
      121 agcatctgtt tttctagttg caagtgtttt cagttttagc tttgctacta cagctagtgt
      181 cgttaacgca gtaacggcac accctgctcc aagtcccatt attgatgtat gggaatatgg
      241 tgtaaatgat aactatggtt attcgaatta ctttgttagt tctccaagta atattggatc
      301 aaaatcttca gtaacgaatt tttggggaac agttaaagct caggataaaa aagattatgg
      361 atgggcatat tcaagtgcaa caaaatcttg gaacgatgtt cgcttaaatg catattggga
      421 ttattataga ttttaaaaat aatttatgaa aacaagagac ccaattataa gattgggtct
      481 cttgtaatat ggtaattgct tatgaaatta aaattatgta tcattggatt cttcttttgt
      541 ttgattgcta caattggtct ggtcactatt agtgataccg aaatccccat acctttacca
      601 attgatggag ctttttctat ccaaggaaaa agtaatcttt ctaataacga aatatacgaa
      661 atggttcgtg acttatcaaa aacggaaaag gttaccattt acaagccaat cgttcagagt
      721 tcgggtcagt tgaagtatgt aaattttgat gatgtaaata atgaacaatt aaagtcagca
      781 ccaatagtag ggatgtatta cacacttggg aaaatggatg tagacagttt gaaaccactt
      841 acgatgacgg gactacaaac agtctatatg gcttatcctt ggtatatagg aggaatatta
      901 caattcactg gaactttaag aatactgtta atgggttcaa tttatttaac tttattagtt
      961 gttttatttg ttgttaggac gaggcagatc aaagaaggag tgatacgacg ttctttgggc
     1021 ttgcctatat acgacttaag aagagagtat ggcatttctc ttatttttga actgattatg
     1081 atggctttat tgatgatttc ttacagttct tttttgggta atggtttctt cacctatagt
     1141 tctaagttat ttttctctct gcttttaacg aattttatac tatttcaaat cattgatctt
     1201 attacttttg ttttattttg gttgacgatt cagattgaaa agcctattga aattattaaa
     1261 aacaaggcaa aaaacaagct tatttttgtc gtatggcttg ctattatttc gattatcatc
     1321 cttgtctcag gagttttttt acaagaaaca aagagtagtc aatctagtat taacatacag
     1381 atacagaatt tagtaccatg ggatacagta aaagactgga gaaggattga gttccttgga
     1441 attgaaagta actctactaa aaatagagaa gttaatgatt cagatggtca gtatctacag
     1501 atagttgctg ccttaaaaaa cttagatttc ttatatatag aacggtcttc agcatatgtt
     1561 ccagatttca tgaaaacttc acatgttata gaaaattttt ctaaacaatt agaaaatgac
     1621 gggataacga atccagaaat aaataaagag ttaatttata tcaaccaaac aggtgctaac
     1681 ctacaaaata aagtgaatgg aacaaactat catcttttag ataataagat agcaaccatc
     1741 tatattcctg agaagtggaa agaaaaccag aaatctattg agaatacagt tgtagcagaa
     1801 caatttattg gaacaaacta tacaagagaa cagcttgcag ttcaaataat tcctgatggg
     1861 gaaaaaattt tttattttaa tgaggatgct gataataatc ttaaaatgaa agatatttta
     1921 ccgctggcaa atgtagcaga tagcaaagat aatatagttg ttgtgttaga tacggacaaa
     1981 atgatggaaa ataataagtt ttctttggct agtaacatac tatataaatc tctttttagt
     2041 cctgaagcgg taaaaaaaat aaatgagatg actgtcctgt tgaatttctc aatgaatcca
     2101 gtagatgttt accaaattgt gaaattaaag attcaatcct tagagcacca aattttactt
     2161 tcacaaatct tgcagaagat aatttacagt attgtcttta tactcattta tcaatatgtc
     2221 caacttttta ttactctaaa acagaatgaa tatgtgaaaa aaatcatttt gggtctgtca
     2281 aaaacatata tagcaatttc aagtctcaaa tattttatga tgactattac aatggttatt
     2341 ctatttacat ttcttatgac agggcaaata gagttgctat atattggagc ggcttctctg
     2401 ctagttttga tgttgtcaat cataatgagt tttagaaaat tatctgaaag ctacactaaa
     2461 attttaaaag gagatgaatc atgacaattg accttttgaa cgtttcaaaa agtttcggct
     2521 caaagaagat ctttacagac ttaaatttaa tatttgaatc tggaaaaagc tatgcattga
     2581 ttggaggttc tggctccggt aaaagtaccc ttcttaatat tataggaaga ttagaaaaaa
     2641 ttgacagtgg gaatgtttta gttgataaac aagatatttg gaagataaaa gaaagaactt
     2701 tttttaaaaa tactgttgga tatgtatttc agaattattc tttaatagat aacaaaacag
     2761 tgtatgataa tctgagcctt atcactaaag acaaaaaaac aataactgac gtacttgaaa
     2821 aagtaggact gtcaagtgac tatttacacc aaaaaatata tgaattgtct ggagggcagg
     2881 ctcaacgtgt agctattgcc agaatgttaa tgaaaccacg taaaattatc ttagcagatg
     2941 aacctacagg agctttagat ggtgagattg gaaaagaaat tattcgttta ctattgaatg
     3001 aaacagctga agataaatat gtcattatag cgacacatga tccagctgtc tataacgaag
     3061 ttgatgtgat aattgacatg aaagatatag ggtataaggt atgaaaaaga aaatttatat
     3121 tgctttgatc tttgtaactg gggtactcgc cattttcttt tttggaaaac aaatgattac
     3181 aaaagaaaat atcaataagc caacggtaga gttaacaatc tatacgcttt cttctagtga
     3241 tacagaaaaa tggaataaag tgagacaagt agaaacggaa gaagctatat acttcataac
     3301 tgtgaaagaa gtatcatctt cagaagaagt attttcaaac atcattgcaa atggagctgc
     3361 tacggggttc ggagttcgtg aagaagaagt gaagaaattc aataatggtt taggagacac
     3421 catagaggac tctaagcata ataaactaat tgaaatagaa ttttttactt tttctgatga
     3481 tggcgcggga tttgtggtag caaactttga ctacggtaaa gaagagttga attctcagaa
     3541 aaaagatata aaagaattat ataaaaaaat atatgaatct tttaaagaaa agaataaatg
     3601 attgtattat aatccttgta ttttgctatg gtttgttaat tgatttttgc atatcttact
     3661 tcaacatact ataaaataaa acataagaaa acgagtatct ccaactacgg aaatactcgc
     3721 tttttgatat ttagaagtgt tttttgcttt gcttcttttg tttcgattaa cgcatggtta
     3781 caaattcttc agatgcagtt gggtgaattg ccacagttgc atcaaagtca gccttggttg
     3841 ctcccatttt gatagcaacg gcaaatcctt gaatcatttc atcaacgccg tagccaattc
     3901 catgaagtcc gacaactttt tcttctgaac cagctgttat gagtttgaaa cgggattctt
     3961 gacggttgcg agtgcaagca gagtacatag atgcaaagct tgatttataa accttgattt
     4021 ggtcttgacc gtattcttta atagcttgct cttctgtcaa accaacagtt ccgatagcag
     4081 ggtgtgaaaa gacaacagtt ggaatagttg agtaatccat ttttgcagta gtttttccgt
     4141 taaagagacg ttcagatagg gtacgtccgg ccttgattgc aactggagtc agttctttct
     4201 cgcccgttac atcacctaga gcatagattc cctcaacaac agtattttgg tattcatcca
     4261 cttggataaa gccacgttcg ttcagagtca ctccagcttt ttcaagttgc aagcccttaa
     4321 cgtttggacg gcgacctgta gcccagataa cttggctagc tgtgtgacta gtaccatctt
     4381 cgaaatgaat ggtaatgccg tcagtagttt tttctaactt gacagggact ttgtgagtgt
     4441 gaagtggtaa gtttgttctt tccatttcct tgaccaaacc ttcaacgatg taggaatcaa
     4501 aactacgtaa aggacgatcg cggcgaacaa agagatctgt cttgacacca aaagtgtgga
     4561 gtacgccagc taattcaacg gcgatataac cagcgcctag aatggcaatt gactctggaa
     4621 gttcttccca ggcaaataca tcatcagaag agccacctag ctcagcacca ggaatattag
     4681 gaatacttgg atgggcacct gtagcaatca cgatatgttt agcacgaatc agttcaccat
     4741 ttacgcttac agtatgagaa tctacaaatt cagcatgacc ttcaatcaag tctacaccgt
     4801 tgcgtttaaa actaccatca taagaagaac gagcgcgatc aatgtaggct tcacgattgc
     4861 gacgtagggt tgcaaagtca aagttaagat cagtagtctt aaagccgtag tcttctccaa
     4921 attgatggaa agtctcagcg atttgcgccc cgtaccacat gattttttta ggaacacaac
     4981 cgacgttgac acaggttcca cctaatttct tttcctcaat aacggctgct ttggctccat
     5041 gttcaccagc acggttcatg gtagcgattc ctccgctacc tccaccgata gcaatgatat
     5101 catattctct cattgaaaac tcctttgtaa tcttttaaat agtagagcgt cttttttgat
     5161 agttatattt tattcttttt ttgatttgat tgcaaaaatc tgctacgcac aaaaaattgc
     5221 aaaaaagctt gccaaataag tcaagatagt gtattatata tctagtacaa tagaaaagca
     5281 aaattccgaa cgattgttta atcctgaact gtattgtttg ctggagggaa atttggtata
     5341 atagcaaagt attgtaaaaa agaaaaggag tttcattatg aagaaaaaga atggtaaagc
     5401 taaaaagtgg caactgtatg cagcaatcgg tgctgcgagt gtagttgtat tgggtgctgg
     5461 ggggatttta ctctttagac aaccttctca gactgctcta aaagatgagc ctactcatct
     5521 tgttgttgcc aaggaaggaa gcgtggcctc ctctgtttta ttgtcaggga cagtaacagc
     5581 aaaaaatgaa caatatgttt attttgatgc tagtaagggt gatttagatg aaatccttgt
     5641 ttctgtgggc gataaggtca gcgaagggca ggctttagtc aagtacagta gttcagaagc
     5701 gcaggcggcc tatgattcag ctagtcgagc agtagctaag gcagatcgtc atatcaatga
     5761 actcaatcaa gcacgaaatg aagccgcttc agctccggct ccacagttac cagcgccagt
     5821 aggaggagaa gatgcaacgg tgcaaagccc aactccagtg gctggaaatt ctgttgcttc
     5881 tattgacgct caattgggtg atgcccgtga tgcgcgtgca gatgctgcag cgcaattaag
     5941 caaggctcaa agtcaattgg atgcaacaac tgttctcagt accctagagg gaactgtagt
     6001 cgaagtcaat agcaatgttt ctaaatctcc aacaggggcg agccaagtta tggttcatat
     6061 tgtcagcaat gaaaatttac aagtcaaggg agaattgtct gagtacaatc tagccaacct
     6121 ttctgtaggt caagaagtaa gctttacttc taaagtgtat cctgataaaa aatggactgg
     6181 gaaattaagc tatatttctg actatcctaa aaacaatggt gaagcagcta gtccagcagc
     6241 cgggaataat acaggttcta aataccctta tactattgat gtgacaggcg aggttggtga
     6301 tttgaaacag ggtttttctg tcaacattga ggttaaaagc aaaactaagg ctattcttgt
     6361 tcctgttagc agtctagtaa tggatgatag taaaaattat gtctggattg tggatgaaca
     6421 acaaaaggct aaaaaagttg aggtttcatt gggaaatgct gacgcagaaa atcaagaaat
     6481 cacttctggt ttaacgaatg gtgctaaggt catcagtaat ccaacatctt ccttggaaga
     6541 aggaaaagag gtgaaggctg atgaagcaac taattagtct aaaaaatatc ttcagaagct
     6601 accgtaatgg tgaccaagaa ctgcaggttc tcaaaaatat caatctagaa gtgaatgagg
     6661 gtgaatttgt agccatcatg ggaccatctg ggtctggtaa gtccactctg atgaatacga
     6721 ttggcatgtt ggatacacca accagtggag aatattatct tgaaggtcaa gaagtggctg
     6781 ggcttggtga aaaacaactg gctaaggtcc gtaaccaaca aatcggtttt gtctttcagc
     6841 agttctttct tctatcgaag ctcaatgctc tgcaaaatgt agaattgccc ttgatttacg
     6901 caggagtttc gtcttcaaaa cgtcgcaagt tggctgagga atatttagac aaggttgaat
     6961 tgacagaacg tagtcaccat ttaccttcag aattatctgg tggtcaaaag caacgtgtag
     7021 ccattgcgcg tgccttggta aacaatcctt ctattatcct agcggatgaa ccgacaggag
     7081 ccttggatac caaaacaggt aaccaaatta tgcaattatt ggttgatttg aataaagaag
     7141 gaaaaaccat tatcatggta acgcatgagc ctgagattgc tgcctatgct aaacgtcaga
     7201 ttgtcattcg ggatggggtc atttcgtctg acagtgctca gttaggaaag gaggaaaact
     7261 aagatgcaga atctgaaatt tgccttttca tctatcatgg ctcacaagat gcgttctttg
     7321 cttactatga ttgggattat tatcggtgtt tcatcagttg ttgtgattat ggctttgggt
     7381 gattccctat ctcgtcaagt caataaagat atgactaaat ctcagaaaaa tattagcgtc
     7441 tttttctctc ctaaaaaaag taaagatggt tcttttactc agaaacaatc atcttttacg
     7501 gtttctggaa aggaagagga aattcctgtt gaaccgccaa aaccgcaaga atcctgggtc
     7561 caagaggcag ctaaactgaa gggagtggat agttactatg taaccaattc aacgaatgcc
     7621 atcttgacct atcaagataa aaaggttgag aatgctaatt tgacaggtgg aaacagaact
     7681 tacatggacg ctgttaagaa tgaaattatt gcaggtcgta gtctgagaga gcaagatttc
     7741 aaagagtttg caagtgttat tttgctagat gaggaattgt ccattagttt atttgaatct
     7801 cctcaagagg ctattaacaa ggttgtagaa gtcaatggat ttagttaccg ggtcattggg
     7861 gtttatacta gtccggaggc taaaaggtca aaaatatatg ggtttggtgg cttgcctatt
     7921 actaccaata tctcccttgc tgcgaatttt aatatagatg aaatagctag tattgtcttt
     7981 cgagtgaatg ataccagttt aaccccaact ctgggtccag aactggcacg aaaaatgaca
     8041 gagcttgcag gcttacaaca gggagaatac caggtggcag atgagtccgt tgtatttgca
     8101 gaaattcaac aatcgtttag ttttatgacg acgattatta gttccatcgc agggatttct
     8161 ctctttgttg gaggaactgg tgtcatgaac atcatgctgg tttcggtgac agagcgcact
     8221 cgtgagattg gtcttcgtaa ggctttgggc gcaacacgtg ccaatatttt aattcagttt
     8281 ttgattgaat ccatgatttt gaccttgtta ggtggcttaa ttggcttgac aattgcaagt
     8341 ggtttaactg ccttagcagg tttgttactg caaggtttaa tagaaggtat agaagttgga
     8401 gtatcaatcc cagttgccct atttagtctt gcagtttcgg ctagtgttgg tatgattttt
     8461 ggagtcttgc cagccaacaa ggcatcgaaa cttgatccaa ttgaagccct tcgttatgaa
     8521 tgagatcaac aagatggaca ctcgtctatc ttgtttttgt atggatttcc ctatcgaaat
     8581 tcattaaaaa tgtgatataa tgaagtgtta gaaaaacagg tttcatttgc ctggaaagga
     8641 aaaattatgt ctgaaaaaaa tttttatatt acaacgccga tttactatcc atctgggaaa
     8701 cttcatatcg gttctgccta cacaactatc gcatgtgatg tcctagcacg ttacaaacgc
     8761 ctgatgggct acgatgtatt ttatctgaca ggtcttgatg aacatggtca gaaaatccag
     8821 cagaaagcgg aagaagctgg tattacacct caagcctatg ttgatggaat ggcggttgga
     8881 gttaaagaac tctggcaatt actagatatc tcatacgata aatttatccg tacaactgat
     8941 gactaccatg aaaaagttgt cgcacaagtc tttgaacgct tacttgctca agatgatatc
     9001 tacttgggtg aatactctgg ttggtattca gtatcagacg aagaattctt tacagaaagc
     9061 cagctggcag aagttttccg tgatgaagct ggaaatgtga ctggtggtat tgctccatca
     9121 ggtcatgagg ttgaatgggt ttctgaagaa tcatacttcc ttcgccttag caaataccaa
     9181 gaccgtttgg tcgaattttt caaagctcat cctgaattta tcacgccaga tggtcgcctt
     9241 aatgaaatgc tacgcaactt catcgagcca ggtttggaag atttggcggt atctcgtaca
     9301 acctttacat ggggagtgcc tgtcccatca aatccaaaac acgttgtcta cgtttggatt
     9361 gatgcccttc ttaactatgc gacagctctt ggctacgctc aagacgaaca tggtaacttt
     9421 gacaagttct ggaatggaac agtcttccat atggtaggaa aagatatcct tcgcttccac
     9481 tctatctact ggccaatcct tcttatgatg ttggatgtta aattacctga tcgtttgatt
     9541 gcccatggtt ggtttgtcat gaaagacgga aaaatgtcta agtcaaaagg gaatgtcgtt
     9601 taccctgaaa tgttggtaga gcgttatgga ctagatccac ttcgttacta cctcatgcgt
     9661 aaccttccag ttggttcaga cggaaccttt actcctgaag actatgtcgg ccgtatcaac
     9721 tatgaattgg ctaatgacct tgggaacctc cttaaccgta cggtttccat gattaataag
     9781 tactttgatg gacaaatccc tgcctatgta gaaggtgtga ctgaatttga tcatgttctt
     9841 gctgaggttg cagaacaatc aatcgcagac ttccatacac acatggaagc agttgactat
     9901 ccacgtgcgc ttgaagcagt ctggactctg atctctcgta ccaataaata catcgatgag
     9961 actgcaccat gggtcttggc caaggatgaa gctcttcgtg accaattggc aagtgtcatg
    10021 agccacttgg cagccagcat tcgtgtagtt gctcacttga ttgaaccatt tatgatggaa
    10081 actagtcgtg cagttttgac tcaacttggt ttggaagaag tttctagtct tgaaaacttg
    10141 agtttggctg acttcccagc agatgtgact gtagttgcca aaggaacacc tatctttcca
    10201 cgtctaaata tggaagaaga aatcgcctat atcaaggaac aaatggaagg caataaacca
    10261 gcagtcgaaa aagaatggaa tccggacgaa gttgagctca aactaaacaa ggatgaaatc
    10321 aagtttgaag actttgacaa ggttgaaatc cgtgtcgcag aagtcaaaga agtgtctaaa
    10381 gtagaaggtt cagataagtt gcttcaattc cgcttggatg ctggtgatgg agaagatcgt
    10441 cagattcttt caggaattgc aaaatactat ccaaatgaac aagaattggt cggcaagaaa
    10501 gttcaaatca ttgctaacct caaaccacgt aaaatgatga aaaaatatgt cagccagggt
    10561 atgattctct cagctgaaca tgatggcaaa ttaacccttc tcacagttga tccagctgta
    10621 ccaaatggaa gtgtgattgg gtaaaagtag acaggactag ttcaggctag ttctgttttt
    10681 ttatttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa
    10741 acacagtttt gaggttgtag atgaaactga cgaagtcagc tcaaaacaca gttttgaggt
    10801 tgtagatgaa actgacgaag tcagctcaaa gcactgcttt gaggttgtag atgaaactga
    10861 cgaagtcagc tcaaaacaca gttttgaggt tgtagatgaa actgacgaag tcagtaacca
    10921 tacataaggt agggcgacgc tgacgtggtt tgaagagatt ttcgaagagt attaactatt
    10981 atgctttaca aactagtgta gaagtgatat actataggtg aagttactag taggtattac
    11041 aaaatagatt agaagggaaa agtgatgaaa gaaactcagc tattaaaagg tgttcttgaa
    11101 ggttgtgtct tggatatgat tggtcaaaaa gagcggtatg gttatgagtt ggttcagact
    11161 ttgcgagagg ctggatttga tactatcgtt ccaggaacta tttatccttt gttgcaaaag
    11221 ttagaaaaaa atcaatggat aagaggcgac atgcgcccgt cgccagatgg tccagatcgg
    11281 aagtattttt cattaatgaa agaaggagaa gagcgtgtct cagtcttttg gcaacaatgg
    11341 gacgatttga gtcaaaaagt agaagggatt aagaatgggg gttaaaccat gaagaaaatg
    11401 aagtattacg aagaaacaag cgctttgcta catgagtttt ctgaggagaa tcaaaagtat
    11461 tttgaggagt tgtgggaaag ttttaatctt gctggatttc tctatgatga agactatctc
    11521 agagagcaga tctatttgat gatgctagat ttctcagaag cagaacgaga tggcatgagt
    11581 gcagaggatt atctaggtaa gaatcctaaa aaaataatga aagagattct caagggagca
    11641 cctcgcagtt ctatcaaaga gtcccttttg acgccaattc ttgtcctggc ggtattacgt
    11701 tattatcaac tactaagtga tttttctaaa ggtcctctct taacagtcaa tttgctcaca
    11761 tttttagggc aacttcttat ttttctgatt ggatttggac ttgtggccac aattttacga
    11821 agaagtttag tccaagattc tcctaaaatg aaaattggca cttacattgt tgttgggact
    11881 atagttcttc tagttgtttt aggatatgta ggaatggcaa gcttcataca agaaggagcc
    11941 ttttatattc cggctccctg ggatagtttg tctgtcttta cgatttcgct agttatcggt
    12001 atttggaatt ggaaagaagc ggtctttcgt ccatttgtca gtatgattat tgcccatctt
    12061 gtggtgggtt ctctgctccg ttattatgag tggatgggaa tttcaaatgt tttccttaca
    12121 aaagttattc ctttagctgt cctctttatt ggcatttttg tcttgttccg tgggtttaag
    12181 aagataaaat ggagtgaagt atagtcaaaa agccgttgca aagcggtttt ttgttataat
    12241 gaagggaaga atgttagaaa ttaaaggaga aaaatttgat gagatacata actcttggtc
    12301 aagatgacaa agaattatca gaaattgttc tcggaatgat gagaataaaa gataagtctg
    12361 taaaagaagt tgaagagctt gtagaaacag cactttctgt tggaatcaat gccttcgact
    12421 tggctgatat ttatggtcgt ggtcgttgtg aagaactgtt aggtcttgtc ctaaaaaatc
    12481 gtccagattt aagagaaaag atgtggattc agtccaaatg tggcattcgc attgaagaat
    12541 ttacctattt tgatttttct aaggactata ttataaaatc agtagacggt attttgcaaa
    12601 gattgaagat tgatcatcta gatagcttgc tccttcatcg accagatgct ttgatggaat
    12661 ctgaccaagt agcagaagcc tttgatctcc tttataaaca aggtaaagtt cgagattttg
    12721 gagtttctaa tcaaaatcct atgatgatgg agttgcttaa aaaagatgtc aagcagccgt
    12781 tagctgttaa tcagctacaa ttgagtgcgg cttttactcc aggatttgaa tcagcttttc
    12841 atgttaatat ggaagatagt caagcagcta tgcgagatgg cagcattttt gaatattgcc
    12901 aattacacga tgtggtcatt caagcatggt ctgtcttgca attcgggtat tttaaaggga
    12961 actttgttgg aaatgagaaa tttcaagctt taaatcaagt acttgatcgt ttagctatta
    13021 aatatggagt aacctcttca actattgcca tttcttggat attgcgttat ccagcaaaaa
    13081 tgcaggcagt cgtaggtaca acaaatccta agcacttgag agaagttagc cgagcggcaa
    13141 actttagctt aacaagaaaa gaatggtatg aaatttatct tgctgcaggg aataatttgc
    13201 cgtaagtagt agcaggtttg aataactcac agtcaaaaag ccgtataaag tggttttttg
    13261 ctataattaa atgaagaatg tagaattaaa ggagaaaaac atgacatttg aagagatcct
    13321 gcctgggtta aaggctaaga gaaaatatgt acgaactggt tggggaggtg cggaaaacta
    13381 tgtccaactc tttgatacca tcgagcaaaa tgggcttgca cttgaaatga caccttattt
    13441 cctaatcaac gtttctggag aaggagaagg tttttccatg tggagcccga cagtttgtga
    13501 tgttttggca acggattggg tagaagtgca tgactaaacg tgtactcatt acaggagtga
    13561 gttcagggat cggattggct caagctcgcc tctttttaga gaagggctat caagtttatg
    13621 gagttgacca aggtgaaaag ccactcttag agggtgattt tcacttttta cagagagatt
    13681 tgaccttgga cttggaacct atttttgact ggtgccctca ggtggatgtt ttgtgtaata
    13741 ctgccggagt tttggatgct tacaaaccac tgttggaaca aacggcgcag gacattcaag
    13801 agatttttga aatcaactac attattccag tagagttgac tcggtattat ttgacacaaa
    13861 tgctggaaaa taaaaaggga atcatcatca atatgtgttc cattgcttct agcctagcag
    13921 gtggaggtgg tcacgcctat acttcatcca agcatgcctt ggctggcttc accaagcagt
    13981 tggctctaga ctatgctgaa gctaggattc aggtctttgg tatcgctcca ggagcagtca
    14041 agacagctat gaccgctgcg gattttgaga caggtggctt agctgactgg gtggctagtg
    14101 aaaccccaat caagcgctgg attgaaccag aggaagtggc agaagtcagt ctctttttgg
    14161 ccagtggaaa ggcatctgcc atgcagggac aaattctgac aatagatggt ggctggtctt
    14221 tgaagtagga ggagttggat ggaaatcaaa aatcactttg gggtctactg tgtttgcttt
    14281 gaaaatggaa agttactttg cattgaaaaa acgagaggcc cttatcaaca tcggtatgat
    14341 ctacctggag gcagtcagca acttggtgaa ggactgacgg aaacgctgac tagagaagtt
    14401 atggaagaga cgggatttac tgttagaagc tactctaatc ctcgaatcta cgatgttttc
    14461 gtcagggaag agttaaaaaa ttttatggtt caccatgtca tagccttgta tgatgttgaa
    14521 atgaatgaga gtgcacctca agttacgatt tcggaagctg tgtctgatgg tgcgaatgat
    14581 tcacttggat atatttggat ggatattcaa gagattacag aagaaaatgc atcgccatta
    14641 gtcttgaagg ttaagtctga attattagga ttgccagaac tggacaagac ttcttacatg
    14701 aattggaagg tgaagtagag ggataatgga actatttaaa acatggaaga aaaatatggt
    14761 tctctatggt cttaaatctc aaattggaac tgtctaccga aacaatgata ggacaacaag
    14821 cttttatgat gttgggaatt ttctatacct agcaggggag ttggattcca gattttggga
    14881 agattttgtt agaaaatatg gtttagatta taagattatt atttcagaaa atactaattg
    14941 gcaagatttt ctgcatcgaa aagtggggct aaattctttt actcgctatt cttttaaaga
    15001 taaggcaaat tttcaagttg aatttttaaa taatctagtt actcatttag aggaaggtta
    15061 caatattgtg cctattgata atcatattta taactgcttt tctacggaag aatggtcaca
    15121 ggatttacag ggggattttg agtcctatca ggattttgtt ttaaaaggtg gatttggctt
    15181 tgtgattctt aaaaataatg aactgattgc tgggatttcc tcagggttag tttaccgtaa
    15241 agcagttgaa gtggaagttg caactagacc aaacgaacaa ggaaatggat ttgctaaaaa
    15301 gcttggtgct gcaatgattc tagagagttt aaatagagat atgtttccac tttgggatgc
    15361 tcataatgag gcttccaaaa aagtagcaga atttttagga tatgagttat ctgaacctta
    15421 tgaagctttt gaactagagg aaattttgat ataattatat atgatattgt atcttttaag
    15481 acgaggtgaa tggctatgag aaatcaactt gcccttagtg gcgaaaaaat tcttgaaaaa
    15541 atttatcctc aactatttca ccacgtcggc atgattcgtg gggaatattt gttgagagag
    15601 cttaatcaaa acatcatatt agcaagttgt cagcaatttg taaaagatta tctagagact
    15661 atctgctccc tgtactcaga tgaagaagtt tggtatcgtt tttctgaatt aacgaataca
    15721 gaagccaatt gtttagtggg gactagagag ttttttgacg aagggcatcc cttgtttggc
    15781 tatagaggaa ccaggcgttt actggcgtgt ttggatgaat ttcaggctga agcacatgtc
    15841 gttacagaag tttatcaaac caaccccaat ctatctgtta tctttccttt tgtcaatgat
    15901 gccgaccaat taaaacaagc tattacagta ttgcgtcagc agggttttac tgggaaagtc
    15961 ggaacgatga ttgaattacc gtcagcgtat tttgacttat ctagtatact ggaaacgggc
    16021 atttcaaaga ttgtagttgg aatgaatgat ttgacttcct ttgtttttgc gactatgaga
    16081 aacagtcaat ggcatgatat ggaaagtcca ataatgttag atatgctaag agatatgcag
    16141 gataaagcaa gaaagaacaa gattgatttt gctgtagcag gctatctgaa tacttctttc
    16201 atacaaaaaa tgaatcagtt gggtatcaag tgcattatcc actacagttc tattccagag
    16261 atttttgatt tagagattga ccatccagat catcttaaac acataaaaga agaaagtaaa
    16321 aaattacaaa ggagtaccca tgataccgca agaaatgtgg aataaataca agcaaatcaa
    16381 tcctttgatc ggagatgaaa tcgatgcttg ggcttttgga gttgaaccag atcttttagc
    16441 ggatttggtg tttaaaggag aaaagacagc aacagcatca gcctatgacc tctatgtact
    16501 agaagacgaa ccacttcccc aagtagggac cttcgatatc attttagata gtcaaaatca
    16561 gtctgtctgc attgtcgaaa ttacaaaggt ttctgttgaa ctcttcaatc aagtttctgc
    16621 gcaacatgcc tttaaggaag gtgagggaga caaatcactt gcctattggc gccaggttca
    16681 tgaggacttt ttcacagact gtttgggtga agtagggctg acttttacac ctgaaagcaa
    16741 ggttgtttta gaagaatttc gcaaggtcta cccactgtag actattagaa ggaagaaagt
    16801 tttggaaatc gctgtccaat cctttttttt caagcaaaat atgatataat aagtttgttt
    16861 gaagaagagc agcagctctt aaacttagaa taggagaaaa ctatgcaagc agttgaacat
    16921 tttattaagc aatttgttcc tgaacattat gatttatttt tagatttgag tcgtgagacc
    16981 aagacttttt ctgggaaagt gaccatcact ggtcaagcac agagtgaccg catctccctc
    17041 caccaaaaag acttggaaat cacctctgta gaagttgcag gtcaagctcg tccatttaca
    17101 gttgaccatg acaatgaagc ccttcatatc gaattggctg aggctggtca agttgaattg
    17161 gttcttgcct tttctggtaa aattacagac aacatgacag ggatttaccc ttcttattat
    17221 acagttgatg gagtcaagaa ggaggtcttg tctactcagt tcgagagcca ttttgcgcgc
    17281 gaagctttcc catgtgtgga tgagcctgaa gccaaagcaa cttttgacct ctctcttcgc
    17341 tttgaccaag cagaaggtga attggccttg tcaaacatgc cagaaatcga tgttgaaaac
    17401 cgtaaggaaa caggtatctg gaagtttgag acaacacctc gcatgtcttc ttacttgttg
    17461 gcctttgttg ctggtgattt gcaaggggtg accgctaaaa ctaaaaatgg taccctggta
    17521 ggtgtctact caaccaaagc acatccactt tcaaatcttg atttctcact ggatatcgct
    17581 gttcgctcta tcgagtttta cgaagattac tatggagtta agtatccaat tcctcaatct
    17641 ctccacatcg cccttcctga cttctcagct ggtgctatgg aaaactgggg tcttgtgacc
    17701 taccgtgaag tttacttggt tgtcgatgag aactctacat ttgctagccg tcaacaagtt
    17761 gcccttgttg tggcccatga attggctcac caatggtttg ggaacctcgt gactatgaaa
    17821 tggtgggatg acctttggct caatgaaagt ttcgctaata tgatggaata cgtctgtgtg
    17881 gataccatcg aaccaagctg gaatatcttt gaagatttcc aaacaggtgg agtacctctt
    17941 gctcttgaac gtgacgctac tgatggcgtt cagtctgtcc acgtcgaagt taaacatcca
    18001 gatgaaatca atacactctt tgacggcgct atcgtctatg ccaaaggaag ccgtctcatg
    18061 cacatgcttc gccgttggct aggagatgct gattttgcta aaggtttgca tgcctacttt
    18121 gaaaaacacc aatacagcaa caccattggt agtgaccttt gggatgccct tggtcaagcg
    18181 tcaggacgtg atgtcgcagc cttcatggat tcttggttgg aacagcctgg ttacccagtt
    18241 cttactgtca aagttgaaaa tgatgtcttg aagatttcac aaaaacaatt cttcatcggt
    18301 gagaacgaag acaagaaccg tctctgggtg gtgcccctca atagcaactg gaaaggcttg
    18361 cctgatacac tcgaaactga aagtatcgaa atccctggct acgcagctct tcttgctgaa
    18421 aatgaaggag ctcttcgtct caacactgaa aatactgccc actatattac cgactatcaa
    18481 ggagacttgt tagaagctgt tcttgctgag ctagagacac ttgataacac aagcaaactg
    18541 caaattgttc aagaacgtcg tttgttggct gaggcagggc acatttctta tgcagacttg
    18601 cttccagttc ttgataaact tgctaaggaa gagtcttatc tggtggtttc agctgtttct
    18661 caagtgattt cagcccttga gcgctttatt gatgaaggaa cggatgctga aacagccttc
    18721 aaaggactgg ttgctaaatt ggctcgtcat aactatgacc gtcttggttt tgaagctaaa
    18781 gacggagaat cagatgagga tgaattggtt cgtcagctgg ctgtttcgat gatgattcgc
    18841 tcaaatgatg cagaagctcg tcaagtcgct agccaaatct tcgcaacaca caaggagaat
    18901 cttgcagaac tcccagcagc tattcgttca caagttctga ttaatgagat gaaacatcat
    18961 gagactaaag acttgttagc actttatctt gatacttata ctcacgcaac agatgctgtc
    19021 tttaaacgcc agttggcagc agctcttgca tacagtacag atgcggataa tatccaaaac
    19081 ttgattactt cttggaagga caaatttgtg gtcaaaccac aggacttgtc tgcttggtat
    19141 taccaattcc tagctcatca agcaactcag aaaacagctt ggtcttgggc gcgtgaaaac
    19201 tgggcttgga ttaaggcagc ccttggagga gatatgagct ttgatagctt tgttatcctt
    19261 cctgctcatg tatttaagac tcagcaacgc ttggcggaat acaaggagtt ctttgagccg
    19321 caactttctg accttgctct tagccgtaac atcggtatgg gaatcaagga aattgcagca
    19381 cgtgttgact tgattagccg tgaaaaagct gcagtcgaag cagttgttct tcaatacgga
    19441 aatgcataaa taagcctaaa ataaaaagaa aaggctcttt gtcaactgta gtgggttgaa
    19501 aaaaagctaa gctcgagaaa ggacaaattt tgtcctttct tttttgatat tcagagcgat
    19561 aaaaatccgt tttttgaagt tttcaaagtt tc
//
LOCUS       DAVKLW010000038        18477 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKLW010000038 DAVKLW010000000
VERSION     DAVKLW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.038"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(83..892)
                     /locus_tag="VW324_001375"
     CDS             complement(83..892)
                     /locus_tag="VW324_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9727006.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(894..1697)
                     /locus_tag="VW324_001376"
     CDS             complement(894..1697)
                     /locus_tag="VW324_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9727007.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1707..3194)
                     /gene="tacF"
                     /locus_tag="VW324_001377"
     CDS             complement(1707..3194)
                     /gene="tacF"
                     /locus_tag="VW324_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9727008.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3530..4237
                     /locus_tag="VW324_001378"
     CDS             3530..4237
                     /locus_tag="VW324_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9727009.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4242..5264
                     /locus_tag="VW324_001379"
     CDS             4242..5264
                     /locus_tag="VW324_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9727010.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLL
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5281..6150
                     /gene="licA"
                     /locus_tag="VW324_001380"
     CDS             5281..6150
                     /gene="licA"
                     /locus_tag="VW324_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9727011.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIGSAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6140..7018
                     /locus_tag="VW324_001381"
     CDS             6140..7018
                     /locus_tag="VW324_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9727012.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7030..7719
                     /locus_tag="VW324_001382"
     CDS             7030..7719
                     /locus_tag="VW324_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9727013.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7815..8663
                     /gene="dprA"
                     /locus_tag="VW324_001383"
     CDS             7815..8663
                     /gene="dprA"
                     /locus_tag="VW324_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9727014.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9552..10595
                     /locus_tag="VW324_001384"
     CDS             9552..10595
                     /locus_tag="VW324_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9727015.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10762..12849
                     /gene="topA"
                     /locus_tag="VW324_001385"
     CDS             10762..12849
                     /gene="topA"
                     /locus_tag="VW324_001385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9727016.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            12955..13314
                     /locus_tag="VW324_001386"
     CDS             12955..13314
                     /locus_tag="VW324_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9727017.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13407..14039
                     /locus_tag="VW324_001387"
     CDS             13407..14039
                     /locus_tag="VW324_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9727018.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            14083..14739
                     /locus_tag="VW324_001388"
     CDS             14083..14739
                     /locus_tag="VW324_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9727019.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14758..15390
                     /locus_tag="VW324_001389"
     CDS             14758..15390
                     /locus_tag="VW324_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9727020.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            15418..15852
                     /locus_tag="VW324_001390"
     CDS             15418..15852
                     /locus_tag="VW324_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9727021.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15864..16901
                     /gene="leuB"
                     /locus_tag="VW324_001391"
     CDS             15864..16901
                     /gene="leuB"
                     /locus_tag="VW324_001391"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9727022.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            16898..17167
                     /locus_tag="VW324_001392"
     CDS             16898..17167
                     /locus_tag="VW324_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9727023.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17171..17530
                     /gene="leuD"
                     /locus_tag="VW324_001393"
     CDS             17171..17530
                     /gene="leuD"
                     /locus_tag="VW324_001393"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9727024.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17692..18315
                     /locus_tag="VW324_001394"
                     /pseudo
     CDS             17692..18315
                     /locus_tag="VW324_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         5785 a         3181 c         3909 g         5602 t
ORIGIN      
        1 tgtgggaata tgtatttcat tttctactaa aattatgttt ttgaataacc tctatcttag
       61 tagtttgtat aatccccctc aatcagtttt tgcgataagc tttaatgcta tgactatacc
      121 actcttgcat ttcttttgat ggtggtgtca tataatcgcc atacatctgg gttaaaaatt
      181 ggtcatattt tttgggaaca ggcaacatac ggccctcaaa ctcagttaaa atcagttctt
      241 taaaggtatc aactgggaag atttctttca tcccttcctt accgatccca actcctcctt
      301 catattgagg aatgttggtt acagcatttt tgactagttg atcaattttc ttgtaaaagt
      361 agcgaggatt gacaaatcgg agagcgtacc agctacataa tctaagaaaa tcttttagtt
      421 tgctatcacc gtgaactgct cgtgattttt tgatataagc tagttgacga agagccacat
      481 acttatagct cttgtcgaca atgctcaagt ctgtaaatcg atcaattggg aagacatcga
      541 tgaaaaggct ggtatcatga cgcttgtact taacatggtc ttctataaca gtagaagtgt
      601 ccaaaatcga tgcgaaatta tggaagtacc aagaagatgt atcgtaggaa agaaccttgt
      661 agcgagggtg attttcttct tcaataatct tcagtaaacg ctcataatcc tcacgataaa
      721 gggaaatatc aatatcatca tcccaaggaa tcataccttt gtggcggatg gctccaagca
      781 tggttccata actgagaaaa taaggaatat catgtttctt acaagtctca tcaatatagt
      841 ccagcagggc tagttgaatt tctttaattt cttttttttc taaatattgc atcctaatcc
      901 tccaatttat aagcgtgaaa ttcatgactg tagaagcgtt tttcttctgg tggtagggtc
      961 atataatctc cataaaattg tgtcaaaata gtatcaaatt tttcaggtgc aggaaggctt
     1021 aaattctcaa agggtaaatc gattgtttta tcaaaggtac cacttgggaa gacttccttt
     1081 tccttaaatt ttgaagggat aaaagccata tactgcccat tttcacgact atatttttga
     1141 atttctttct cgattttatt tgcaaaataa cgaggagaaa ctggtcgaag gagcaaccaa
     1201 aaggctgttc gtatccaatc ttttaaaagg ctatccttat agacaatatt tttatgttta
     1261 ctgaaagaca gcagtttgaa gctttccagt ttataacaag tatcaatgac cttaggatca
     1321 tcaaagcgat ccatagggaa aatatcgata aagataccag actcataggt ttttgtattt
     1381 cgagtatcaa taattttagt cgtactgtcg gttatcttga taaagttgtt aaagtagttc
     1441 ttatcagttt ctaaggatag gagttcatac ttgctttttt ccttttgaaa aatgttaata
     1501 aatcgttggt agtcttctct aggcatggat aaatcaatat cgtcgtccca agggataaag
     1561 ccctcatgtc gaaccgcccc aatcagagta ccgtagttaa taatatagtt gatattgtgc
     1621 tttttacaga gagtatcaat ataatccaaa atttctaatt caatttgttt ggcatcttca
     1681 acggttagtt gtttcatttt aaactcctat gattttttaa atttattttt taaggctaga
     1741 acatggttta aaaattcata gaaaatgcta tcttttgtga agacaagtag actaatataa
     1801 gagatagctg atagcaagat aatcaaacca gtattaatca aaaatggcaa attaatgacc
     1861 atatccacag gatacacgaa attaatcagg aaataaattg ctacaaagga aagtgaaaag
     1921 agagagtatc gaacagtata gctaaagata tgtcccaagt ggatgagttg ttttctatgg
     1981 atgaaaatga tatagaaaac aagtagagaa gcctctgata acatagttgt cagtaagtag
     2041 tattcgggag ccacgatacg gttgaaaaag aggagactat tcaagcccaa attgagtagc
     2101 ccagcaaaga ctgtataaac tgtgatacgt ttttcatagc cattggtaaa gagaatttgg
     2161 gaaccaagaa tggtatctaa ggccaggata atcgtacgaa aagcgaagag agaggtcaag
     2221 atgccgcctc cgatatattt ttcactaccg taaagtagga tggcatttgg tcctaaaacc
     2281 atgagtccaa aactcagtgg aatgataaag aagttaaaga ttcgactacc tctattaacc
     2341 agagaaacat aggcttcttt gtctcctttc cccagatagt aactgagacg aggcacactc
     2401 actccaattg cacctgttac aaccccagct ataacggtca caattcgctg agctatggta
     2461 tagtaactaa cgttgacatc aatccctgtt ttaacgagga agaggcgatc taaaaaagtg
     2521 aagagcatat tggcattggc aaagactaac atggctgtca gagggagaaa gagtggttta
     2581 aaatcactta ggtgaatctt aacaagtttg atgtctcttt taatccaaaa ataactaatc
     2641 aggtagttaa tcagcgtcga taaactcatc acaagtgtat agacaacaat atcgtgttca
     2701 tttttaacaa ataagaaaat agagaccagc atcaggatac ggatgaaggc agttttgtaa
     2761 aagagaaaac tgtaattttc cagagcttca ttgacccatt cgattgaaaa aatctgggca
     2821 atgagttgaa tccccataac aaggtagacc tttttgatga ttggattatc agtaaagaag
     2881 agaggatagg ctaggatata gacagcagtg gtcaaaatcg tacaagcgat gcacaaataa
     2941 aaaagactag aaaaggttct gttaagatct tttttgttat ccttgacatt actgatagcc
     3001 cttaaaccgt agttatagac accataagtt gcaaagggca agaaaaatga caaaatagtg
     3061 tcgactgagt tgaagtaacc atagtcagtt cggtccaaga cacgcgcgac ataggttcca
     3121 gttaggatgg gaaaaataat attcaagaca cgaattccca tgtaagatag agcatttaat
     3181 tttatacttt tcattcaatt tacctcgttt ttcattatat cataaagtta gctaataaga
     3241 aatgaagggc agtaagtcaa gtaatcactt tgaagtttca aatcttaagt tttaagtttt
     3301 ctttaaggaa agtatattat tctgaaggac tctaaaattt cgcagccatt tattagtaat
     3361 tgctacagaa ttcctagtca ttactagaaa tggactagtt tctttgaata atagaactgc
     3421 ataattctcc tattctagaa ggggaggacc agtatttctt ttatgatagg actagattgt
     3481 ggtataatag agagaataag tttttttagt aagacaaagg agaaaataga tgatttatgc
     3541 aggaattctt gccggtggaa ctggcacacg catggggatc agtaacttgc caaaacaatt
     3601 tttagagcta ggtgatcgac ctattttgat tcatacaatt gaaaaatttg tcttggagcc
     3661 aagtattgaa aaaattgtag ttggtgttca tggagactgg gtttctcatg cagaagatct
     3721 tgtagataaa tatcttcctc tttataagga acgtatcatc attacaaagg gtggtgctga
     3781 ccgcaataca agtattaaga acatcattga agccattgat gcttatcgtc cgcttactcc
     3841 agaggatatc gttgttaccc acgattctgt tcgtccattt attacacttc gcatgattca
     3901 ggacaatatc caacttgccc aaaatcatga cgcagtggac acagtggtag aagcggttga
     3961 tactatcgtt gaaagtacca atggtcaatt tattacagat attccaaatc gtgctcacct
     4021 ttatcaagga caaacacctc aaacattccg ttgcaaggac ttcatggacc tttatggatc
     4081 tctttctgat gaagagaagg aaatcttgac agatgcatgt aaaatctttg tgatcaaagg
     4141 aaaagatgtg gctttggcca aaggtgaata ctcaaatctg aagattacaa ccgtaacaga
     4201 tttgaagatt gcaaaaagta tgattgagaa agactagtaa aatgattaat caaatttatc
     4261 aactaactaa gcctaagttt atcaatgtca aatatcagga agaggctatt gaccaagaga
     4321 atcatatcct tatccgtccc aactacatgg ctgtctgtca tgcggatcag cgttactatc
     4381 agggaaaacg tgatcccaag attttgaata aaaagcttcc aatggcaatg attcacgagt
     4441 catgtggaac cgtcatttct gacccgaccg gaacctacga ggttggtcaa aaagttgtca
     4501 tgattcccaa tcagtctcct atgcagagtg atgaagaatt ctatgaaaac tacatgacag
     4561 ggacccattt cttgtctagt ggatttgatg gctttatgag agagtttgtt tctctcccta
     4621 aagatcgtgt ggtggcttat gatgctattg aagatacggt tgcagccatt acagagtttg
     4681 tcagtgtggg catgcacgct atgaatcgtc tattgactct tgctcatagc aagcgggagc
     4741 ggatcgccgt tattggagat ggaagtttag cttttgtggt tgccaatatt atcaactata
     4801 ctttgccaga agcagagatt gtggttattg gtcgtcattg ggaaaagttg gaactcttct
     4861 catttgccaa agaatgctat attacggata atattcctga agatttggcc tttgaccatg
     4921 cttttgaatg ttgtggtggt gatggtactg gaccagctat taatgacttg attcgctaca
     4981 ttcgtcctca gggaacgatt ctcatgatgg gagttagcga atataaagtc aatctcaata
     5041 ctcgcgatgc cttagaaaag ggcttgctct tggttgggtc atctcgttct ggtcgcattg
     5101 attttgaaaa tgctatccaa atgatggaag tcaagaaatt tgccaatcgt cttaaaaata
     5161 tcctttatct agaagaacct gtaagagaaa ttaaagatat tcatcgtgtc tttgcaaccg
     5221 atttaaacac agcctttaaa acagtgttta agtgggaagt ataagtactg gaggttaatt
     5281 gtggagaaaa tcattaaaga aaaaatttct tccttactta gtcaagaaga ggaagtcctc
     5341 agtgttgaac aactgggtgg aatgaccaat caaaactatt tggccaaaac aacaaataag
     5401 caatacattg ttaaattctt tggtaaaggg acagaaaagc ttatcaatcg acaagatgaa
     5461 aagtacaatc ttgaactact aaaggattta ggcttagatg taaaaaatta tctttttgat
     5521 attgaagctg gtatcaaagt aaatgagtat atcggatctg cgattacgct tgattcaacg
     5581 tcaatcaaga ccaagttcga caaaattgct ccaatattac aaactattca tacgtctgct
     5641 aaggaattaa gaggagaatt tgctcctttt gaagaaatca aaaaatacga atccttgatt
     5701 gaagaacaaa ttccttatgc caactatgaa tctgttagaa atgcagtctt ctccttagag
     5761 aaaagactgg ctgacttagg tgttgacaga aaatcttgtc atatcgattt ggtgcctgaa
     5821 aactttatcg aatcacctca aggacgactt tatttgattg actgggaata ttcatcaatg
     5881 aatgatccaa tgtgggattt ggctgccctc tttttagagt ctgaattcac ttcccaagag
     5941 gaagaaactt tcttatctca ctatgagagt gaccaaacac cggtttctca tgaaaagatt
     6001 gctatttata aaattttaca agatactatt tggagtctat ggactgtata taaggaagag
     6061 caaggtgcag attttggtga ctatggtgtg aatcgttacc aaagagctgt taaaggtttg
     6121 gcttcttatg gaggatcaga tgaaaagtaa aaacggagtt ccttttggcc ttctctcagg
     6181 tattttctgg ggcttgggtc taacggttag tgcttatatc ttttcgattt ttacagattt
     6241 gtcacccttt gtggtggctg caactcatga ttttttgagc atctttatct tactagcttt
     6301 tctcttggta aaagaaggga aagttcgcct ctcaattttc ttaaatattc gcaatgtcag
     6361 tgttatcatc ggagccttgc tagcaggccc tatcggtatg caggccaatc tttatgcagt
     6421 taagtatatc ggaagttctt tagcttcatc tgtatcggct atttaccctg cgatttcagt
     6481 tctattggct ttcttctttt tgaagcacaa gatttcgaaa aatactgtat ttgggattgt
     6541 cttgattatt ggagggatta ttgctcagac ctataaggtt gaacaggtta attctttcta
     6601 cattgggatt ctttgtgctt tggtttgtgc tattgcatgg ggaagtgaga gtgttcttag
     6661 ctcttttgcc atggaaagtg aattgagtga aatcgaagcc ctcttaatcc gtcaagtaac
     6721 ttcgttcttg tcctatcttg tgattgtgct cttctctcat cagtcattta ctgcagtagc
     6781 caatggacaa ttgctaggtc tcatgattgt ttttgcagcc tttgatatga tttcctactt
     6841 ggcttattat atcgctatca atcgcttgca accagccaag gctacaggct tgaacgtgag
     6901 ctatgtagta tggacggtct tgtttgcagt tgttttcttg ggtgcaccgc tagatatgct
     6961 gaccattatg acgtcacttg tcgtcattgc tggagtttat attattatta aagaataaag
     7021 gagattcgtg tgaaagccat tatcttagca gcgggattgg gaactcgctt gcgtcctatg
     7081 actgaaaata cccctaaagc cttggttcag gttaatcaaa aacctttgat tgagtaccaa
     7141 attgagtttc tcaaagaaaa aggaatcaat gacatcatca tcattgttgg ttatcttaaa
     7201 gaacaattcg attacttgaa agagaaatac ggtgttcgtc tcgttttcaa tgataaatac
     7261 gctgactaca ataactttta ctctctctat cttgtaaaag aagaattggc caacagctat
     7321 gttattgatg ctgacaatta tctctttaaa aatatgttcc gcaatgattt gacacgttcg
     7381 acttatttta gtgtttatcg tgaagattgt accaacgaat ggttcttggt ttatggagat
     7441 gactacaagg ttcaagacat tattgttgat agcaaggcag gtcgcatcct tagtggtgta
     7501 tccttctggg atgctccaac tgcagaaaag attgtcagct ttatcgacaa ggcttatgta
     7561 agtggtgaat ttgttgatct ctattgggac aatatggtta aggataatat caaagagcta
     7621 gatgtctatg ttgaagaatt agaaggcaat agcatttatg agatcgatag tgtccaagac
     7681 tatcgtaaat tagaagaaat tcttaaaaac gaaaattaaa gattccaaca tctgacaaaa
     7741 tagtcggatg ttttttgatt ttttacgaac ttttacgaat agatagatga gtagaaaaag
     7801 aaatggagtt atttatgaaa atcacaaact atgaaatcta taagttaaaa aaatcaggtt
     7861 tgaccaatca acagattttg aaagtgctag aatacggtga aaatgttgat caggagcttt
     7921 tgttgggtga tattgcagat atctcaggtt gccgtaatcc agccgttttt atggaacgtt
     7981 attttcagat agacgatgcg catttgtcga aagagtttca aaaatttcca tctttctcta
     8041 ttttagatga ctgttatcct tgggatttga gtgaaatata tgatgcgcct gtacttttat
     8101 tttacaaggg aaatcttgac ctcctgaaat tcccgaaggt agcggtcgtg ggcagtcgtg
     8161 cttgtagcaa acagggagct aagtcagttg aaaaagtcat tcaaggcttg gaaaatgaac
     8221 tggttattgt cagtggtctg gccaagggca ttgacacagc agctcatatg gcagctcttc
     8281 agaatggcgg aaaaaccatt gcagtgattg gaacaggact ggatgtgttt tatcctaaag
     8341 ccaataaacg cttgcaagac tacatcggca atgaccatct ggttctaagt gaatatggac
     8401 ctggtgaaca acctctgaaa tttcattttc ctgcccgtaa tcgcatcatt gctggacttt
     8461 gtcgtggtgt gattgtagca gaggctaaga tgcgttcagg tagtctcatt acgtgtgagc
     8521 gagcaatgga agaaggacgc gatgtctttg ctattcctgg tagcatttta gatggactat
     8581 cagacggttg ccatcatttg attcaagaag gagcaaaatt agtcaccagt gggcaagatg
     8641 ttcttgcgga atttgaattt taaaaatgac ctaagctaga attctaagaa aaaatcaatt
     8701 ttaagagaaa atgaacccaa catttccata ataaaacgca tattagcaag tttttaacac
     8761 ttgataatat gcgttttttc taagtggatt agtagagtag aggatttttc tcatataata
     8821 ctcttcgaaa atctcttcaa actacgtcag cttccatctg caacctcaaa acagtatttt
     8881 gagctgactt cgtcagtctt atctacaacc tcaaagcagt gctttgagca acctgtggct
     8941 agcttcctag tttgcgcttt gattttcatt gagtataagg gaaagtatag tgaattgaaa
     9001 taagatgtga acaactctat caggaaagtc aaattgattt atagaaatat tttagcagcc
     9061 aaggtgcact gttatagatt caatacacta taatttagtg taattgagaa aggagaaatg
     9121 attgtgattg atgttggcta ggttatgttc aatgattcct accgtctcaa atcttgtcag
     9181 taaggaaaaa taaattcttc aaaagtagag attacaaggc ttgtttaaga aagaattcaa
     9241 agaccttgac aaataaaaat aaaatggtta ttataaaaaa tggtctgaaa tagatgatga
     9301 tacttttcga aaatctcttc aaactacgtc agctcagctt tgccttgctg tgttttgagc
     9361 aagctacggt tagcttccga gtttgatttt catttactag aaatgaaact gatgagagat
     9421 atcagtagac atttgagtca ggatattatg gaaaatgata aaaagagctc gtgagattgg
     9481 catatcagac tactaaagta ttgagtttgt taggatttta gcgactagtt agctgggaaa
     9541 ggaagatatt tgtgacaaat aataaactgt attcgttgat agaatttaga aataaaatat
     9601 atgaagaatt agaactttcc agaagtgatt tagcgatttt actatgtgcc atgcttatcg
     9661 cctctatcgg attaaatatg gattcgactc ccgtgattat tggagccatg ttaatctctc
     9721 ctttgatgac acctattctg ggagtggggc tctctctagc tatatttgat tttaaattgt
     9781 taagaaaatc ttttaaaata ttagctattc aaattcttgc cagtctaata gcttcaacac
     9841 tttattttta tctttctccc atttcgtatg ctagttcgga gattgttgct agaacctctc
     9901 cgactatttg ggatgttctc attgcttttg taggagggat agcaggtatt attggtgcta
     9961 ggaaaaaaga gaccaataat attgttcctg gtgttgctat tgcaaccgcc ttgatgcctc
    10021 ctctttgtac agtaggttat gctattgctt ctgctaatct aaaatttatc ataggctcct
    10081 cttacctatt cctcatcaat tgtagcttta ttgtcattgc gacttatata ggtgttaggt
    10141 tgatgatggt taagaaacat tattttaaag ataatgaaga agactctaaa atgcgtagga
    10201 ttttgcttct agttgctgtt ttgctgatga ttccgagttt catctctgca acgactttag
    10261 tgagagaaac gttgaaaaaa gagtccctta agaaatttat atcagagcag tttcaggggt
    10321 ataatatttt gaaaaaaacc tattctaaaa agactcatac cctaaagcta accatttcag
    10381 gaaattattt gacagaagaa gaactcgata tgatttcaag taagagaggt gactatggtt
    10441 taagtgatgt ttctgttcaa gtttcacaat tgtctgattc agaacaactt agtaaggaag
    10501 agctggtgga gtatttcttc cagtatatca aggataagga agcaaaagaa aaggagaaag
    10561 ccaataaatt tgatacagag tctgaggagc aataatttct tgagaatagc tggtttctct
    10621 cgtgagtctt ctatgtatat caaaggaaga ctgaggtttt aagtataaaa cttttcttct
    10681 attatagtag ctaagtcttg acagggttca aaaaatggtt tacactttat aaagtttatt
    10741 actttgaaaa ggtgtgatac tgtggctacg gcaacaaaaa agaaaaaatc aacagttaaa
    10801 aaaaatctag tcatcgtgga gtcgcctgct aaggccaaga cgattgaaaa atatctaggc
    10861 agaaactaca aggttttagc cagtgtcggg catatccgtg atttgaagaa atccagtatg
    10921 tccgtcgata ttgaaaataa ttatgaaccg caatatatta atatccgagg aaaaggccct
    10981 cttatcaatg acttgaaaaa agaagctaaa aaagctaata aagtttttct cgcgagtgac
    11041 ccggaccgtg aaggagaagc gatttcttgg catttggccc atattctcaa cttggatgaa
    11101 aatgatgcca accgtgtggt cttcaatgaa atcaccaagg atgcagtcaa aaatgctttt
    11161 aaagaacctc gtaagatcga tatggacttg gtcgatgccc aacaagctcg tcggatcttg
    11221 gatcgcttgg tagggtattc gatttcgcct attttgtgga agaaggtcaa gaagggcttg
    11281 tcagcaggtc gcgttcagtc cattgccctt aaactcatca ttgaccgtga aaatgaaatc
    11341 aatgccttcc agccagaaga atactggaca gttgatgctg tctttaaaaa gggaaccaaa
    11401 caatttcatg cttccttcta tggagtagat ggtaaaaaga tgaaactgac cagcaataac
    11461 gaagtcaagg aagtcttgtc tcgtctgacg agtaaagact tttcagtaga tcaggtggat
    11521 aaaaaagagc gcaagcgcaa tgctccttta ccctatacca cttcatctat gcagatggat
    11581 gctgccaata aaatcaattt ccgtactcga aaaaccatga tggttgccca acagctctat
    11641 gaaggaatta atattggttc tggtgttcaa ggtttgatta cctatatgcg taccgattcg
    11701 actcgtatca gtcctgtagc gcaaaatgag gcggcaagct tcattacgga tcgttttggt
    11761 agcaagtatt ctaagcacgg tagcaaggtc aaaaacgcat caggtgctca ggatgcccat
    11821 gaggctattc gtccgtcaag tgtctttaat acaccagaaa gcatcgctaa gtatctggac
    11881 aaggatcagc tcaagctata tacccttatc tggaatcgtt ttgtggctag ccagatgaca
    11941 gcggccgttt ttgataccat ggctgttaaa ttgtctcaaa atggagttca atttgctgcc
    12001 aatggtagtc aggttaagtt tgatggttat cttgccattt ataatgattc tgacaagaat
    12061 aagatgttac cggacatggt tgttggagat gtggtcaaac aggtcaatag caaaccagag
    12121 caacatttca cccaaccgcc tgcccgttat tctgaagcaa cactgattaa aaccttagag
    12181 gaaaatgggg ttggacgtcc atcaacctat gcgccaacca ttgaaaccat tcagaaacgt
    12241 tattatgttc gcctggcagc caaacgtttt gaaccgacag agttgggaga aattgtcaat
    12301 aagctcatcg ttgaatattt cccagatatc gtaaacgtga ccttcacagc tgaaatggaa
    12361 ggtaaactgg atgatgtcga agttggaaaa gagcagtggc gacgggtcat tgatgccttt
    12421 tacaaaccat tctctaaaga agttgccaag gctgaagaag aaatggaaaa aatccagatt
    12481 aaggatgaac cagctggatt tgactgtgaa gtgtgtggca gtccaatggt cattaaactt
    12541 ggtcgttttg gtaaattcta cgcttgtagc aatttcccag attgccgtca tacccaagca
    12601 atcgtgaaag agattggtgt tgagtgtcca agctgtcatc agggacaaat tattgagcga
    12661 aaaaccaagc gtaatcgcct cttctatggt tgcaatcgct atccagaatg tgaatttacc
    12721 tcttgggaca agcctgttgg tcgtgactgt ccaaaatgtg gcaacttcct catggagaaa
    12781 aaagtccgtg gtggtggcaa gcaggttgtt tgtagcaaag gcgactacga ggaagaaaag
    12841 attaaataag aggaggggcc cgaaagtaaa tttcaggctc tttttggtta gaacttgaca
    12901 aaatacatca ttttatgcga aactagaaga agattatttt aactaggaga agttatgcgt
    12961 cttatctatc tgataattgg ttttttatca ctggccttgg ctattgttgg ggttgtttta
    13021 cccttgttgc ctacaacacc tttccttttg ttgtctattg cttgtttctc cagaagttcc
    13081 aagcgattcg aagactggct ttatcatacc aagctctatc aaacatatgt agctgatttt
    13141 cgcgagacta agtctattac gcgtgaacga aagaaaaaaa tcatcgtatc catttacatt
    13201 ttgatgggga tttctattta ttttgcccct ctcttaccag tcaaaatcgg tctgggtgct
    13261 ttgaccatct ttatcactta ttatctcttc aaggtcattc cagacaaaga atagttaaaa
    13321 cagtagttat ttaccttgat aaaattgaaa gcatattcat aacaatatga tataataaat
    13381 ttgaagtaat attcaaggag aatcaaatga tttacgaatt ttgtgctgaa aatgtgactt
    13441 tacttgaaaa agcgatgcag gctggagctc gtcggattga actctgtgat aatctagcag
    13501 ttggtgggac aacacctagc tatggagtga ctaaggcagc ggttgaactg gcagctaact
    13561 acgatacaac catcatgacc atgattcggc cacgtggtgg tgactttgtc tataatgacc
    13621 tagaaattgc tatcatgcta gaagacattt gtttgactgc tcaggctgga agtcaagggg
    13681 ttgtatttgg agctttaact gctgataaaa agttggataa gcctaatctg gaaaagttaa
    13741 ttgctgcatc aaaaggaatg gaaattgtct ttcacatggc ctttgatgaa ctaagtgatg
    13801 aagatcaacc ggaagctatt gactggctca gtcaagccgg tgtcactcgt atcctaactc
    13861 gtgctggtgt gtctggcgac tccttagaaa aacgttttgt tcactatcac agaattttgg
    13921 agtacgctaa aggtaaaatt gaaattctac caggtggggg gattgacctt gacaaccgtc
    13981 aaacctttat tgaccaggtg ggggtaacac aattgcatgg tactaaggtt gttttttaaa
    14041 aaatagaaag gaactgctag ccttgggtag cagttttcac ttatgtttga aatttttaaa
    14101 tcctatcaat ttaatcaaga aaaggctcat gattatggtt ttatagaaaa tagcgaagtc
    14161 tggacatata gttgccagat tttgcaaggt gactttgtca tgactgtgtc catcactgct
    14221 gataatgtga actttcaagt ctttgaccaa gagactggtg acctctatcc tcacgttcat
    14281 atggaaagca tgaggggaag ttttgtcgga aatgtccgtg aggcttgtct ggagattctt
    14341 taccagattc ggaaggcttg ttttgatgtg caagatttta tctgtcatca gactaagcgt
    14401 atcatgactc aagttcagga aaagtatgga aaccagttgg agtatctgtg ggaaaaatcg
    14461 cctgatacag ctgtattgcg ccatgaaggc aatcaaaagt ggtatgccgt cttgatgaaa
    14521 atctcttgga ataagctgga aaagggcaga gaaggacaag tggaagcagt caacctcaag
    14581 catgaccaag tagctaattt gctttcacaa aaggggattt atccagcctt ccatatgagc
    14641 aagcgctact ggattagtgt gtcccttgat gatactttat cagatgaaga agtactggaa
    14701 ttgatagaaa aaagttggaa cttaacctct aaaaaatgaa atattttaat aattttcatg
    14761 aactttcaat tagctaaata ttctttactg aagaaatttt tagaaaatat aggatttacc
    14821 acactagagg aatatggtgc catcttcaaa tacctgattg agaatgtcaa gacggatcgt
    14881 cagatcatct gttcgcctca ctgtcatgat gacctcggaa tggcagtggc aaatagcctt
    14941 gctgctgtca agaatggtgc aggacgtgtt gaagggacta tcaatggtat tagggagcga
    15001 gctgaaaatg ctgctttgga agaaattgca gtggctctca atattcgcca agattactac
    15061 caagtagaaa ccagtattgt cctaaatgag accatcaata cgtcagaaat ggtttctcgc
    15121 ttctctggta ttccagttcc taaaaacaaa gccgtcgttg gtggcaatac cttctcccac
    15181 gaatctggta ttcaccaaga tggagtcctt aaaaatcctc tcacttatga gatcatcaca
    15241 cctgaattgg ttggtgttaa gattccgctt ggaaaattat ctggtcgcca tgcttttgtt
    15301 gagaaactga gagaattggc cctagatttt acagaagagg atatcaaacc actctttgct
    15361 aagttcaagg cactggccga taagaagtaa gaaatcacag atgcagatcc tcgagctttg
    15421 gtagctggaa ccatggttga aaatccagaa ggcttccact ttgatgattt acaacttcaa
    15481 actcatgcag ataatgacat tgaagcgctc gttagcctag ccaatatgga tggtgagaaa
    15541 gtcgaattta atgcgacagg gcaaggttcc gttgaagcaa tctttaacgc tatcgataag
    15601 ttctttaacc aatctgttcg tttggtgtcc tacactatca atgcggtaac agatggaatc
    15661 gatgcccagg atcgggtttt ggtcactgtt gaaaacagag atacagaaac catctttaat
    15721 gcagcagggc ttgattttga tgtgttgaag gcttctgcta ttgcctatat aaacgctaat
    15781 acctttgttc aaaaagagaa tgcaggtgag atgggacgca gtgtttctta ccacgatatg
    15841 cctagtgtgt aaaggagaag gctatggcaa agaaaatagt agctctagta ggagacggaa
    15901 ttggcccaga aatcatggag gctggtttag aagttctgga ggctctagct gaaaaaacag
    15961 gttttgacta tgagattgac agacgaccgt tcggaggtgc agatattgat gcagcaggac
    16021 ctcccttacc tgatgaaacc cttaaggcaa gtagggaagc agatgctatc ctactagcag
    16081 ctatcggtag tcctcagtat gatggagcag cggttcgccc tgaacaaggc ctgatggctc
    16141 tccgtaagga actcaatctt tacgctaata ttcgtcctgt aaaaatcttt gacagtctca
    16201 agtatttgtc accactcaaa ccggaacgaa tttctggtgt agactttgtc gtggtgcgtg
    16261 aattgacagg cgagatttac tttggagatc atattcttga agagcgcaaa gcgcgtgata
    16321 tcaacgacta tagctatgag gaagtggagc ggattattcg caaagccttt gaaattgcaa
    16381 gaaatcgcag aaaaatcgtt actagtatcg ataagcaaaa tgttctagcg acctcaaaac
    16441 tctggcggaa agtagctgag gaagtcgcac aggatttccc agatgtaacc ttggaacacc
    16501 agctggtaga ctcagctgct atgcttatga ttaccaatcc tgctaagttt gatgttattg
    16561 taacggagaa tctttttgga gatattttat ctgatgaatc aagcgtctta tctggtacac
    16621 ttgaggttat gccatcagcc agtcattctg aaaatggacc aagtctctat gaacctattc
    16681 acggttcagc acctgatatt gcaggtcaag gaattgccaa tcctacttcc atgattttat
    16741 cagttgccat gatgttgaga gatagtttcg gacgttatga ggatgcagag cgtatcaaac
    16801 atgctgttga gacaagtctg gcggcaggaa ttttaacgag agatatagga ggtcaggctt
    16861 caacaaagga aatgacggaa gctattattg caaggttatg aagttagacg aaaaaattac
    16921 tctagtcctt ttgatttgga atgtcatcat tttcttgatt tatggcattg acaaattcaa
    16981 ggcaagaaga agaacttggc gcatccaaga gaaaatctta ctcattttag cccttacttg
    17041 tggtggtttt ggggcctggt tagctggaat cacttttcac cacaagactc gaaaatggta
    17101 ctttaaaaca gtttggtttc ttgggatggt gaccacacta gtagccttat attttatttg
    17161 gaggtaatgg atggcagggt cttcgaggga acacgctgct tgggttctag cggactatgg
    17221 ttttaaggtc gtgattgcag gatctttcgg tgacattcat tacaataatg aactcaataa
    17281 tggcctgttg ccaatcgttc agcctagaga ggttagagag aaactagccc agctaaaacc
    17341 aaccgaccag gtaactgtgg acttggaaca acaaaaaatc atctcaccag ttgaagaatt
    17401 caccttcgag atagatagcg agtggaaaca taaactccta aatagtttgg atgatatcgg
    17461 tattaccttg cagtatgaag agttgattgc tgcttatgaa aaacaacgac cagcctactg
    17521 gcaggattag aaaaaataga aaaggaaata tagtaaactg aaataagatg taaacaaatg
    17581 aattagagct taacatccat ttccagcaat tttttagaaa ctacagtgga ctattctgga
    17641 ttcaacacat tataaaatta tgacaaaaca cattcacaag aaggctacga cattttaaaa
    17701 ggtgagggcg gatgtatcgt ttgccctact aaagttggtt acattatcat gaccagcaac
    17761 aaggcaggac ttgagcgtaa gttcgcagcc aaagaacgta agcgtaacaa accaggtgtt
    17821 gttctctgcg gtagcatgga tgaacttcgc gctttagcgc aactcaaccc agaaattgaa
    17881 gcattctact aaaaacattg gatgaagata ttcttcttgg ttgtatcctt ccttggaaac
    17941 cagaagcctt tgaaaaactc aaagcatacg ggaatggccg tgaagaactt atgactgatg
    18001 tacgtggtac tagctgtttt gttatcaagt ttggaaaagc aggtgaacaa ttggctgcca
    18061 agctttggga agaaggtaaa atggtctacg cctcatatgc ttcaatgaca aaacgattga
    18121 aactcgctat gagagcaagg tgtaatggtg tctatggtcg ataaggacgg caaactcatc
    18181 ccagaacaag gaggagcacg ttcaacttca ccagcaccag ttgtgatccg taaagggctt
    18241 gacattgata aaatcatgat gcacctgtca gatactttta actcatggga ctaccgtcag
    18301 gttgagtatt attaagataa aaaaagaagt ctagtgttat gagatattaa agctcctaac
    18361 actggacttt ttgtttagaa tttcttttct ttttctatag gatatggtat tctatgtaga
    18421 aaatatatgt taataagtaa tgccaatatt taaacatcat tagtaaaagg agttaga
//
LOCUS       DAVKLW010000039        18455 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKLW010000039 DAVKLW010000000
VERSION     DAVKLW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.039"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..928
                     /locus_tag="VW324_001395"
                     /pseudo
     CDS             1..928
                     /locus_tag="VW324_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            1324..2187
                     /locus_tag="VW324_001396"
     CDS             1324..2187
                     /locus_tag="VW324_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9727025.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            2473..2673
                     /locus_tag="VW324_001397"
     CDS             2473..2673
                     /locus_tag="VW324_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9727026.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            2740..3405
                     /locus_tag="VW324_001398"
     CDS             2740..3405
                     /locus_tag="VW324_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9727027.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3477..4703
                     /locus_tag="VW324_001399"
     CDS             3477..4703
                     /locus_tag="VW324_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9727028.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTTLYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(5056..5130)
                     /locus_tag="VW324_001400"
                     /pseudo
     CDS             complement(5056..5130)
                     /locus_tag="VW324_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5201..5857
                     /locus_tag="VW324_001401"
     CDS             5201..5857
                     /locus_tag="VW324_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9727029.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            5847..6056
                     /locus_tag="VW324_001402"
     CDS             5847..6056
                     /locus_tag="VW324_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9727030.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPTIVERFLKFL
                     PVSIIFALILSRVVTGKVGNLPQIK"
     gene            6274..7104
                     /locus_tag="VW324_001403"
     CDS             6274..7104
                     /locus_tag="VW324_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9727031.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            7258..8112
                     /locus_tag="VW324_001404"
     CDS             7258..8112
                     /locus_tag="VW324_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9727032.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            8212..9585
                     /locus_tag="VW324_001405"
     CDS             8212..9585
                     /locus_tag="VW324_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9727033.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            9578..10639
                     /locus_tag="VW324_001406"
     CDS             9578..10639
                     /locus_tag="VW324_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9727034.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            10641..11333
                     /locus_tag="VW324_001407"
     CDS             10641..11333
                     /locus_tag="VW324_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9727035.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11362..11931)
                     /locus_tag="VW324_001408"
     CDS             complement(11362..11931)
                     /locus_tag="VW324_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9727036.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            12066..12680
                     /locus_tag="VW324_001409"
     CDS             12066..12680
                     /locus_tag="VW324_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9727037.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12682..14328
                     /locus_tag="VW324_001410"
     CDS             12682..14328
                     /locus_tag="VW324_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9727038.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14340..15626
                     /locus_tag="VW324_001411"
     CDS             14340..15626
                     /locus_tag="VW324_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9727039.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15678..16211
                     /locus_tag="VW324_001412"
     CDS             15678..16211
                     /locus_tag="VW324_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9727040.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            16289..16759
                     /gene="nrdI"
                     /locus_tag="VW324_001413"
     CDS             16289..16759
                     /gene="nrdI"
                     /locus_tag="VW324_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9727041.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(17052..18313)
                     /locus_tag="VW324_001414"
                     /pseudo
     CDS             complement(17052..18313)
                     /locus_tag="VW324_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
BASE COUNT         5442 a         3350 c         4024 g         5639 t
ORIGIN      
        1 gacagattat gatgagaaaa ttggtagttt taatacatat gaaatcgaac aaacggtatc
       61 gaagattctg agggtaaaac ctaatggaaa gattatttta aagtcaacag ttccgttcgg
      121 cttttcaaac aaattaaaaa ggctgtttga tacaaaaaat atcatttttg tccctgaatt
      181 tttgagagaa ggttgttcta tatatgataa tttatatcca agtcgcatag ttgttggaga
      241 tgagacagtt gaaggaagaa aaattgcaga gttgttcctt tcgattagta ctcatagtac
      301 tgccaatatt aaaaatgtta tgttagtttc tcctactgaa gcagaagcaa ttaagctttt
      361 ttctaacaca ttcttagctc tccgtgttgc tttttttaat gaactagatt cttttgctga
      421 gaggagaagt ttaaatgctg aagttgtaat aaaaggtgtt tgtttagatc caagaattgg
      481 aaatttttat aataatcttt cttttggatt tggaggatat tgtcttccca aagatactaa
      541 acaataaaaa aaagaattta tagaaataaa tgccccagtg atagaagcga ttgatatttc
      601 aaacacaaat agaaaacagt tcatagttaa acaaatattg gaacgaaagc caaagatagt
      661 aggaatatat aaattgggga tgaaatataa ttcagataat tacaaagagt cagctatttt
      721 aagtataatt aatgaactat tgattgtcgg tataaaaatt ttagtatatg aaccaaattt
      781 aaacgttagt atagataacg taatttttga aaagaatttt gaactattta caaaacaaag
      841 tgatctaatt gttgcaaata gatgggatag gggccttgaa gcttataaag ataaagttta
      901 cacaaggggg atctggataa gagattaact attgatatta aaaaggcaat aatttataaa
      961 atttgtacaa aaaaatgtta aattttcaca ttttcatagt atactgttta atagaaagta
     1021 ggttctttgt aaccgctcca ttataaaatc ctttcactct tccggtatac tattaggaaa
     1081 aacataactg gaggatattt tgagctcaca cctcatcgtt ccagtggctg ttccccaagc
     1141 tcgtgtactg cccaaaaaag ttagacattc tatttagaac gaggattgag ttctgtattg
     1201 tacaaggctc ggtcctttta gagtcagctt aaggctggct tttttatttt ttctaactat
     1261 cagaatattt tgacaaatga acacaaataa tgatataata tgcaaaagct aggaggtggt
     1321 aggatgattg aaaaaatgga attgggggaa ttttacaagg aattacgttt ggcgagaaag
     1381 ctcaagcagt cagatgtggc ttgtgacggg ctaacagctt ctcagttgtc caagtttgag
     1441 ctagggcagt ccatgttgtc tgctgataag ctcatattag ccatccaagg gatcaatgtg
     1501 acctttgatg agtttgggca caagctcaac aactatcaag aatctccaca tatgcagatt
     1561 ggtagaaagg ttgtggatcg ctttgctcat caagatatag ctggcttaga gcaactgttg
     1621 gaggaagtcg agcaagaaca gatggcagag acttatcgtc gtttgaatgc tattgtaatc
     1681 aaagatgcca tccactcact agataaaagc tacccactag cagaggagga tagcgagttt
     1741 ttgaccacct atctctatgc tatcgagtct tggacctggt ttgaactcta tctcttttgc
     1801 aatactctgc ccttcttgag caatcaagat ctgatctttt tatcaacctc cttactcgaa
     1861 aaatccaaag aatttaaaga gttagtacac aatcgattgt atatgaagca aggactctta
     1921 aatatcatct cagagctcat ggagcgcaaa cttttttctt atatcccaat ctttgaagcc
     1981 gagctggaga gtatgctccg tccatacgat gtttttgaga aagtgttgtg gcagttttta
     2041 aagaagatga gcattttcct tcaaactaag ggaagcaatc aaaaagagat tgaacacttt
     2101 atccaatctc tgcaagtatt agaaaatcca caattaatag ccctttttga attgcgtttg
     2161 cagcaataca aaaagcttat cgattagtta gaaatggcaa aataataata atctcagcaa
     2221 cgattttcaa taatgttaaa atttgtttct tcatatttct ccactcctca ttagtagtaa
     2281 ctttattata ccagagaagt aatcaaattc accaaaatcg caaatctgaa atttctataa
     2341 gaaaaaatat caaatatgcg attttctaaa ataagccaat tttcgtgtta tactgtactt
     2401 gtaaagcact tgaagcaaat cctaggtcgc agaagtggtt tacaaatgaa gataattgaa
     2461 ggagtgtaaa agatgttaaa tttacaattt gcagaaacaa tggaattgac agaagctgag
     2521 ttggagatag tttatggagg ggaatttggg aataatgctg ttatcccagc cggtgcttgg
     2581 ggagggttcg ggacaccttg gtcaatcact aatttctgga agaaaaattt taacgataga
     2641 cctgactttg attctgaccg ccgtcgttat taatatccaa agcccttgcc ttttcttggg
     2701 taggggctgt ctttatttca tagtatagga ggaaatagaa tgaaaaagat aatctcacac
     2761 cgctacttta ttatagtttt tctactagtt attgctgacc agaagttcag tgttctagtt
     2821 ttacgcagcg accttgttac tgggctatct gactttgcct attatctgtc ggatatgatg
     2881 ttgaattttc ttgtggtttt atttgctctt attgctatga tttggtcggg gaaatggcag
     2941 aaaatcaata gtagaaagtt taaaggatcc tatcttttct attccttcct agctctgctt
     3001 gcttttgttg tttggaattt cgttaccttt tttcttttcc cacctactcg aaatgaaatt
     3061 tcttatcaac atgctgctcc gacttttaca ggggctacag catttttgat gtattttttc
     3121 tatcctgtga ttgcaggtcc catttttgaa gagataatcc atcggggatt ggtgatgaca
     3181 gctctggaaa aaggaaagaa atgggggcta gatgtacttg gctctgctat tttatttggg
     3241 gtctcgcaca ttagtaatca cggttgggtc ttgacagact ttgtcttcta tatgggtgga
     3301 ggtcttatat ttgcagtctt atttagaatg acaaagtcaa tttattggcc tattggactg
     3361 catatagtct acaatggtat tggtcagctt ttgatgttac tgtaattttg gttgttagta
     3421 gtatcgcggt ctattcttgg ggaaagattc ccatttgaac ttaagaagga gttgtaatga
     3481 aagtatttct tcaaaataga gattttaggc aattaactat caaccagtgg atttcaacgc
     3541 ttggggatac gattttttat ctggccttcc tgaattatgt ggcagatgcg tcttttgccc
     3601 ctttggcgat tttacttatc acgatttcag aaacccttcc ccaagttcta caaatctttc
     3661 tgggagtttt ggcggatttt caacatcatc gtgtcttgaa atacacagtc attagtttta
     3721 caaaattttt gctttactct atagtgtccc tatcgctttc agggcagtct ttttccttgt
     3781 tgctagtaac ctttatttgt atgattaacc tcttgtctga cacattgagt tatttttcag
     3841 gcgccatgct cactccgatt ttcattagaa ttattgggca agatcatctg gcagaagcta
     3901 ttggatttaa acagtcaact gttagtttag tgaaaacaat cagtaatatc ctaggaggag
     3961 tcttactagg tattctatcc atccagttta tttccttact gaacgctctt acctttttaa
     4021 tcgcattttt aggtatcctc ttcataaaaa ctgacctctt gaaagtagaa aaaacgatta
     4081 gctatcaaga aggactctct gtaaaatcct tttgccagca tttgctccaa tcatcgaaat
     4141 tgatatggaa tatgaataag gtgctcttgg ttttgtttat tatctctact agtcaagcag
     4201 tgataaatgt tacagttcct gtctctactc tatttttgag gaatcaaccc tttttgaatt
     4261 tacaaacagg tcaatctctt gccttgctat ccacttttga actgtcagcc ctgattgtcg
     4321 gaagccttgt aagtggctat ctgcaacata ccatttccat aaaaacaact ctctatgcct
     4381 cacttgtcat ccagttgctt cttctagtag gatttgccac agttcgtttt gactggattc
     4441 tcatctttag taccttggat gcctttttcg caggtgttct ctctcctaga ttgcaagaac
     4501 tcgtctttaa acaaatacct gaggagtcaa tgggagcagt tcaatcttct atcagtgcca
     4561 ttacggttgt tttacctagc ttatttacaa tatctttggt aaccattgct actagctttg
     4621 gaactctggc agttagcttt gttttattgc tatttcttct agttgccttt gttatgcttc
     4681 tgaatattcg tgaaagtatt tagtggagaa tttctataat atatgtttga gactggattg
     4741 ccagtctttt tatgatggtc aaaatcaaca gactttttct cttttctgaa tgtgttatag
     4801 tagactgaat ctaaaatagt acgaaacaat tgctaaaaca tttatagaaa ttaattttac
     4861 tttcccaatc gatttgttct catcttattt caatctgcta tataatagtc catgctgtgg
     4921 ctggattctt ttcagcctac ttatactctt cgaaactctc ttcaaaccac gtcagcttca
     4981 ccttgccgta tatgtgttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt
     5041 tgagctgact tcatcagttc tatctgcaac ctcaaaacag tgttttgagc aacctgcggc
     5101 tagtttccta gtttgctctt tgattttcat tgagtattat taagacaata tgaggagaaa
     5161 gatgaaagaa aaaggatttt aggagggggc gcaggcagcc atgccaacgg ctcttggcta
     5221 tgtcagtatc ggcctggcct gtggaattat cggtgcgccc tatgtgacac ctgttgagat
     5281 gggcttgatg agtctctttg tttatgctgg gagtgcccag tttgccatgt tggcactgat
     5341 tgtggttcaa gctcctgtgg cagctattgc tatgacggtt tttctaatca atttgcgtct
     5401 ctttttgttg agcttacacg catcgactta tttccgtcat accagtcttt ggcacaatat
     5461 cggtatgtct agtatcttga cagatgagac ctatggcgtt ttgatgggcg aattagccca
     5521 tacagataag gtcaatccga tgtggatgca cgggaacaat ctcaacagct atgtggcttg
     5581 gtttgtggga acagtggtcg gaacggctct aggtgacctg ttgccaaatc cagaaatctt
     5641 tggtttagat tttgccctgg ttgggatgtt tatcgggatt tttgcttcgc aattccagat
     5701 gatgcaaaga cggatttctg tccgcaatct gctcattatc ttagcagttg ttgcagtgtc
     5761 cttcttttta cttttgacag tggtgtctca gtcactagct gttctgtttg cgacgctact
     5821 tggttgtagc atggggatgg ttttagatgg tcagtaagta tcttttatta gcagttattt
     5881 tctctggtct tgtgacctgg attccccgta tgattccctt catcttggtc aagtataagg
     5941 gcctgcctac gattgttgag cgttttttga agtttttgcc tgtttccatt atctttgcct
     6001 tgattctttc aagagtagtg acaggtaagg ttgggaacct tcctcaaatc aaatgactgg
     6061 acttcttagc agtctttcca acagcttggg tagcttttcg ctaccgcaat ctagtcggta
     6121 cggttctctt tggagtagtc ttgattgccg tcttgcgttt ggtcttttaa ataagtcata
     6181 aagaaaactt atcacagaga tagatatcat ataatggcgt aattactttt tttctgataa
     6241 gattataagg tattctattt tggaggaaat gacatgaaaa aaatcgttaa atactcatct
     6301 cttgcagccc ttgctcttgt tgctgcaggt gtgcttgcgg cttgctcagg tggtgctaag
     6361 aaagaaggac aagcagctag caagaaagaa atcatcgttg caaccaatgg atcaccaagg
     6421 ccatttatct atgaagaaaa tggcgaattg actggttacg agattgaagt cgttcgcgct
     6481 atctttaaag attctgacaa atatgatgtc aagtttgaaa agacagaatg gtcaggtgtc
     6541 tttgctggtc ttgacgctga ccgttacaat atggctgtca acaatcttag ctacactaaa
     6601 gaacgtgcgg agaaatacct ctatgccgca ccaattgccc aaaatcctaa tgtccttgtc
     6661 gtgaagaaag atgactctag tatcaagtct ctcgatgata tcggtggaaa atcgacggaa
     6721 gtcgttcaag ccactacatc agctaagcag ttagaagcat acaacgctga acacacggac
     6781 aacccaacta tccttaacta tactaaggca gatttccaac aaatcatggt acgtttgagc
     6841 gatggacaat ttgactataa gatttttgat aaaatcggtg ttgaaacagt gatcaagaac
     6901 caaggtttgg acaacttgaa agttatcgaa cttccaagcg accaacaacc gtacgtttac
     6961 ccacttcttg ctcagggtca agatgagttg aaatcgtttg tagacaaacg catcaaagaa
     7021 ctttataaag atggaactct tgaaaaattg tctaaacaat tcttcggaga cacttatcta
     7081 ccggcagaag ctgatattaa ataatttctt gaaatcatcc attctgagta agatgggtgg
     7141 tttctcagtt tttagggata tagttttaaa ctatatatct gactatctaa taacaatttg
     7201 tgaaatcaaa caaattgtga gatactagta cggtattatt tttaaggaga aagaatcatg
     7261 aaaatcaaaa aatggcttgg tctagcagcc cttgctacag tcgcaggttt ggctcttgca
     7321 gcttgcggaa actcagaaaa gaaagcagac aatgcaacaa ctatcaaaat cgcaactgtt
     7381 aaccgtagcg gttctgaaga aaaacgttgg gacaaaatcc aagaattggt taaaaaagac
     7441 ggaattacct tggaatttac agagttcaca gactactcac aaccaaacaa agcaactgct
     7501 gatggcgaag tagatttgaa cgctttccaa cactataact tcttgaataa ctggaacaaa
     7561 gaaaacggaa aagatcttgt agcgattgca gatacttaca tctctccaat ccgcctttac
     7621 tcaggtttga atggaagtgc caacaagtac actaaagtag aagacatccc agcaaacgga
     7681 gaaatcgctg taccaaatga cgctacaaac gaaagccgtg cgctttactt gcttcaatca
     7741 gctggcttga ttaaattgga tgtttctgga actgctcttg caacagttgc caacatcaaa
     7801 gaaaatccaa agaacttgaa aatcactgaa ttggacgcta gccaaacagc tcgttcattg
     7861 tcatcagttg acgctgccgt tgtaaacaat accttcgtta cagaagcaaa attggactac
     7921 aagaaagcac ttttcaaaga acaagctgat gaaaactcaa aacaatggta caacatcatt
     7981 gttgcgaaaa aagattggga aacatcacct aaggctgatg ctatcaagaa agtaatcgca
     8041 gcttaccaca cagatgacgt gaaaaaagtt atcgaagaat catcagatgg tttggatcaa
     8101 ccagtttggt aataagaaac agggaggtgg gagagaaaat tccacctctt gcttttgtat
     8161 agagtataga ttgtaaagaa gactattcgt tcatagaaag gtagagagaa tatggttttt
     8221 cctagcgaac aagaacagat tgaaaaattt gaaaaggatc atgtagtcca acattatttt
     8281 gaggttttgc gtaccttgat ttctaagaaa tcagtctttg cccagcaggt tggactcaag
     8341 gaagtcgcaa attatctggg tgagattttc aagcgtgttg gagctgaagt ggagattgat
     8401 gagagctata cagcgccctt tgtcatggca catttcaaga gttcgcgtcc agatgccaag
     8461 accttgattt tctataacca ctatgacact gtgccagcgg atggggatca ggtctggaca
     8521 gaggatcctt ttacgctttc ggtccgcaat ggcttcatgt atgggcgtgg ggttgatgac
     8581 gacaagggtc atatcacagc tcgcttgagt gctttgagaa aatatatgca gcaccatgat
     8641 gatttacctg tcaatatcag ctttatcatg gagggagcgg aggaatcggc ttcaacagac
     8701 ctagataagt atttggaaaa gcatgcagac aaactccgtg gggcggattt gttggtctgg
     8761 gaacaaggga ccaaaaatgc cttggagcag ctggaaattt ctggtggcaa taaggggatt
     8821 gtgacctttg atgccaaggt aaaaagcgct gatgtggata tccactcgag ttatggtggt
     8881 gttgtggaat cagctccttg gtatctcctc caagccttac agtctcttcg tgctgcggat
     8941 ggccgtatct tggttgaagg cttgtacgaa gaagtacaag agcccaatga acgagaaatg
     9001 gccttgctag aaacttatgg tcaacgaaac ccagaggaag ttagtcggat ttatggattg
     9061 gagttgcctc tcttacagga ggagcggatg gcctttctaa aacgtttctt tttcgagcca
     9121 gcgcttaata tcgaaggaat ccagtctggt tatcaaggtc agggtgttaa gactatttta
     9181 cctgcagaag ccagtgccaa gctagaggtt cgtctggttc cgggcctaga accgcatgat
     9241 gttctggaaa aaattcggaa acagctagac aaaaatggct ttgataaggt agaattatac
     9301 tataccttgg gagagatgag ctatcgaagc gatatgagcg caccagccat tctcaatgtg
     9361 atcgagttgg ccaagaaatt ctatccacag ggcgtttcag tcttgccgac gacagcgggg
     9421 acaggaccta tgcatacggt ctttgatgcc ctagaggtac caatggttgc attcggtcta
     9481 ggaaatgcca atagccgaga ccacggtgga gatgaaaatg tgcgaatcgc tgattatcac
     9541 acccatatcg aattagtaga ggagctgatt agaagctatg agtagagata ttatcaagtt
     9601 agatcagatc gatgtgactt ttcaccaaaa gaagagaacc attacagcgg ttaaggatgt
     9661 gaccattcac atccaagaag gggatatcta cggaatcgtt ggatattctg gagcaggaaa
     9721 atcaaccctt gtacgggtga ttaatctctt gcaaaaacca tctgcaggga aaattaccat
     9781 tgacgacgat gtgatttttg acggcaaggt gaccttgacg gcagagcagt tgcgtcgtaa
     9841 acgtcaagat atcggaatga ttttccagca ttttaacctg atgagccaaa agacagcaga
     9901 ggagaatgta gcctttgccc ttaaacactc tgaactcagc aaggaagaaa agaaggctaa
     9961 agtagctaag ttgttggact tggttggttt ggcagatcgt gctgaaaact acccttcaca
    10021 actatctgga gggcaaaaac agcgtgtggc aattgcgcgt gccttggcca atgatccaaa
    10081 aatcttgatt tcagacgagt caacttctgc ccttgatccg aagacaacca agcagatttt
    10141 ggccttgttg caagatttga accaaaaatt aggcttgact gttgtcttga ttacgcatga
    10201 aatgcagatt gtcaaagaca ttgccaaccg tgtggcggtt atgcaggatg ggcatttgat
    10261 tgaagagggt agtgtgcttg aaatcttctc agaccctaaa caacctttga ctcaagactt
    10321 tatctcaaca gctacaggta ttgacgaagc catggtcaaa atcgagaagc aagaaatcgt
    10381 ggaacacttg tctgaaaaca gtctcttggt gcaactcaag tacgctggat cttcaacaga
    10441 cgagccactt ttgaatgaat tgtacaagca ttatcaagta atggctaata ttctctatgg
    10501 gaatatcgaa atcctcgatg gtactcctgt tggagaattg gtggtggtct tgtcaggtga
    10561 aaaagcagcg ttggcaggtg cccaagaagc cattcgtcaa gcaggcgtac agttaaaagt
    10621 attgaaggga ggacagtaag atggaatcat tgattcaaac ctatttgcca aatgtctata
    10681 agatgggttg gtctggtcag gcaggctggg gaacagctat ctacctaacc ctctatatga
    10741 cagttctttc cttcattatc ggaggcttct tggggctagt ggcaggtctc tttctcgtct
    10801 tgacagcgcc aggtggtgtc ttggagaata aagtcgtatt ctggatttta gacaaaatta
    10861 cctcaatttt tcgtgcgatt ccctttatca tcctcttggc aatcttgtca ccactttctc
    10921 acttgattgt taaaacaagt atcgggccaa atgcagccct tgtcccactt tcttttgcag
    10981 tctttgcctt ctttgcccgt caggtgcagg ttgtcttggc tgaattggat ggcggtgtca
    11041 ttgaggcggc tcaagcgagc ggagcgactt tctgggacat cgtgggtgtt tacctatcag
    11101 aaggtcttcc agatttgatc cgtgtgacga ctgtgacctt gatttccctt gttggggaaa
    11161 cagctatggc cggtgcggtt ggagctggtg gtatcggtaa cgtagccatc gcttatggat
    11221 ttaaccgcta caatcacgat gtgaccatct tggcaaccat tattatcatt ttgattatct
    11281 ttgcaatcca attcttggga gatttcttga ctaagaaatt gagtcataaa taaaaagagc
    11341 cgcgtggctc tttttaactg atcagatttt ctgtgcaaat tttttactca aggcttgtcc
    11401 aatcaaggca cccactaggg ctccgatgac aatacttgcg ataaatagaa ggacagttcc
    11461 agggtttgga gcgaccatga tacggtcgat atattcttgg gattttcctc ttgccagaag
    11521 agtagccata taggctttgg gcgcaatcca cataagcaag attggtcctg ttgtactaaa
    11581 ggcgaaaata atgaaagaaa ggaagttctt tgttttgtcc ttgtattttc ctaaatgagc
    11641 tacttcatct gctaggaggc cacagataat tccaggaagg aaggaaccag caccgtgttt
    11701 agttcccaag aaaaagaggg caatgacaag gccgatagtg gtaatggctc caaagcgcgg
    11761 aacttttgcg actaggatca tatagacgct accgccgaca agggcagtaa aggcaggcgc
    11821 ataaaacatg tttccagttt ggtcaaagag attgcccaaa aggacaccaa tccccatgca
    11881 gaggaagtaa agaactgcaa aaagcagtgt agttaagata gatttcttca tgaattgtct
    11941 cctgataatt ttttcacaat tctcattgta ccacggtttg gtgggattgt aaatggatac
    12001 tagaagtttt tgaaaacgct tgaaatatga tataatggtt tcatagttat ttttagggga
    12061 atatcatggg tagattttta gactttgtct ttaatcgttt ctttttaggg atgattgcga
    12121 cagccttctt ttggctatta actttagcag gagggattat ccttggtcta gcgccggcta
    12181 gtgccacctt gatgagctta tatgcagaac atggttatag ctttcgggaa tacagtttga
    12241 aggaggcttg gtctctttac aagcaaaatt ttgtctcaag caacctgatt ttctatagct
    12301 ttttaggtgt gggtctagtt ttgacctatg gtttgtatct cttggtgcaa ttgcctcatc
    12361 agaccattgt tcatttgatt gcgacccttt tgaatgtcct agtagttgcc ctgatctttt
    12421 tggcttatac agtatcttta aaattacaag tttattttgc cttgtcctat cgaaatagtc
    12481 tcaaattatc cttgattggc atctttatga gtctagcagc tgtggccaag attctccttg
    12541 ggactgtgct acttgtagca attggttact atatgcctgc cctgctattt tttgtaggaa
    12601 ttgggatgtg gcatttcttt atcagtgata tgttggaacc tgtctatgaa atcatccatg
    12661 aaaaattggc gacaaaatag aatgaagcag ttttggctac atacgcttct aagaacctat
    12721 agttcagtga tgatcattat cattgcgagt tttgcaatct tactctctta cgctgactgg
    12781 gattcacgtg aaaaggaagc ccagagagta gcccagcgtg taactgctag aacagtgagt
    12841 gaaattgaat attaccatag agagtcaacc cagatagctc aggctttagt tgaaaaccaa
    12901 gctcgtattg agggaatcta taaatacttt agtcttagca tgccagacta tttttactgg
    12961 caattagagc ggaaagcttc gccttatata tcagtctctc tgtatgaaaa tgttgatgac
    13021 ctctatgttc gaaatgattt tgtaactgga gtggccattg cttttcaaga ttacaaggaa
    13081 gtctatgttt ctactaaaga caaacgtagt ggagaaaaaa tcagggctga ggatttcaaa
    13141 ccagcaggaa atagttttgc cattccggtg tcagatccag tgtcagatca agatttagga
    13201 gtgatttaca tctccttgga tcctgctgtt ttataccatg ccattgataa tactagaggt
    13261 catactccga tggcagtaac agtgacctca ccttttgata cggagatttt tcatattggt
    13321 gagacagttg ataaggagag tgaaaattgg ctagttggct taacttctca tggatatcag
    13381 gttcaggtgg cagttcctaa aaactttgtt ttacaaggaa cagtgactag ctctgctttg
    13441 attgtgggtt tgagccttct ctttattgtc attctttatc tgactttgag gcagactttt
    13501 gctaattacc aaaagcaggt agtggattta gtagaatcca ttcaagtcat tgctcaaggc
    13561 gaagaggggc gtcggattga catttccgag aaagatcagg aattactcct aatcgcggag
    13621 acgaccaatg atatgttgga tcgattggaa aagaatatcc atgatattta ccagttagag
    13681 cttagtcaaa aagatgccaa tatgcgagcc ttgcaggcgc aaatcaatcc tcattttatg
    13741 tataatacgc tggagttctt gcgcatgtat gcagttatgc agagtcaaga tgagttggca
    13801 gatatcattt atgaattcag tagtctcttg cgtaacaata tttccgacga aagagagacc
    13861 ctcctcaaac aggaattaga attttgccgt aaatacagct atctctgcat ggttcgctat
    13921 cccaagtcca ttgcctatgg tttcaagata gatccagagt tagagaatat gaagattccc
    13981 aagtttacct tgcaaccgct ggtagaaaac tatttcgcgc atggtgttga ccacaggcgg
    14041 acagataatg tgattagcat caaggctctt aaacaggatg gttttgtgga aattttggtg
    14101 gtcgataatg gtagaggaat gtcggctgaa aagttggcaa atatccgaga aaaattaagt
    14161 cagagatatt ttgaacacca agccagctac agtgatcaaa ggcagtctat cgggattgtc
    14221 aatgtacacg agcgttttgt gctctatttt ggagaccgct atgccattac gatagagtct
    14281 gcagagcaag ccggtgttca gtatcgtatt acaattcaag atgagtagaa agggagaaaa
    14341 tgtataaagt attattagta gatgatgagt acatggtgac agaaggtctg aagcgtttga
    14401 ttccctttga taagtgggat atggaggtcg tcgcaacagc cagtcatgcc gatgaagctc
    14461 tagaatatgt tcaggaaaat cctgtcgatg tcatcatttc cgatgtcaat atgccagaca
    14521 aaacagggct tgatatgatt cgggagatga aagagatctt accagatgct gcctatatcc
    14581 tgctctcagg ttatcaggag tttgattatg taaaaagagc aatgaacctt agtgtggtgg
    14641 actatttggt caagcctgtt gataaggtag agctgggaaa tctgctggag aagattgcag
    14701 gtcagctcgg cgagagaggg aagaaaagtc agactcttag tcaagaatta gacgaggctg
    14761 gatttgttag ttatttaggg gataaggaga attggtggat aggtctatcc aaggaaaaac
    14821 aaggttcctt cgccattccc tactatgtct tgggccaaga ctggcagatt ttcatttctg
    14881 accaacccct agatggttta gtcgttacac cttttgaagc tccttatcaa gaacactttg
    14941 aacgctggaa gctgaatgct gagaaaaccc tcttttacgg ttctgtaaat ctgcagcagt
    15001 ctgagagtct ctttgcctat tacgaaccga tttatagggt tatcattcag ggaaatctca
    15061 atcaaatcgt agaagagtta aatctcttgg agaaggtagt tcttgaaaat acgccgcgtg
    15121 ttccgattac taaacagctt tttattcagt ttgtcatgga tgttttccat ttatttgaac
    15181 atctcaaagc tgatgacctg acagacatcg tcaaaaccat tcatgctatt caatccttcg
    15241 atgaattggt tccttatatc aaggaaactc tgaccagctt tttcggtcaa taccgtatga
    15301 atgaaaatgt ggtcagtgtg ctggaagtca ttggtcgtga ttaccaaaaa gagctttccc
    15361 tcaaggatat cagtaaggct ctctttatca atcctgtcta tctagggcag ttgattaagc
    15421 gtgaaaccga ttcgactttt gcagagttac taaacaaaca acgtattaag gctgcccaac
    15481 aacttttgct ttcaactagt gacagcatcg aagatatttg ttatgctgtt ggttacagta
    15541 accttggata tttctataaa gtttttcgaa aattgtgcgg aaaatcgcca aaagcctacc
    15601 gaaaacaggt agaaactata ctataagatt tgtattcctt tacaaaaggt gctataatat
    15661 tatcaggagg ttctatcatg aaaaagaaac caatttatct atgggtcttg ctaatcttgt
    15721 ctgcccttat ttcagttccg tctctgtttg gaattgtgag tcccttgcct agtaaagaag
    15781 cccttcgtgc tgctcagaaa caagttgcag gggtcaatgc tcagcaatta gaagaccagc
    15841 ttaattatac ctatagagta gcagaagcat ctcattctat ttttaatgtt gctttgattg
    15901 tgctatctac tattttagtg gtcgtagcga ttgtcttcct tgttcgtaaa aatttgcaat
    15961 atgcaaacta tacttacgtt ggttatgttt tgttagctat tattggttcg atttatggct
    16021 atgttggttt acaagacgct gtgcagttgg ttcaagatga gagcatgcgt ttgacagtga
    16081 gtattggttc aaaagctgtt agcattttct atatcgtcat caatgttctc ttcctagccc
    16141 ttgtcttcta taagatgtgg cgacaacaga aagccttggc agaagaagag gaaacagaag
    16201 agcttaccta agtattgaca aaaaagaact atcaagatac aatcttggta gttgtttttt
    16261 gattatagat aaaatcatgg aggtacatat gaagacaatt tctttagttt atattagttt
    16321 gagtggcaac actgaaagtt ttgtgacgcg cttgaaagac tatctcttgt cccagtacaa
    16381 agggattgag gttcaaaaga ttcatatcaa ggatttagtc aaggaaggca aaaatttcta
    16441 tgaaatggac catccctatg tcgctttttt gccgacctat ctcgaaggtg ggaatggcgt
    16501 ggataacgga gatgttgaga ttttgacgac accggtggga gattttattg cctatggtaa
    16561 caatgctagt aagtgttttg gtgtggttgg gtcaggaaat cgtaacttta ataaccaata
    16621 ctgcctaaca gccaagcaat acagtcaacg ctttggcttc cctgtattgg ctgactttga
    16681 aatgcgaggc atgctggaag atatcaaaca tgtcgcagct attatcgcag atttgtatga
    16741 gttggaaaag gagaattaat actcaatgaa aatcaaagag caaactagga agctagtcgc
    16801 aggttactca aagcacagct ttgaggttgc agatagagct gacgtggttt gaagagattc
    16861 tcgaatagta taaagttctt ataagaggtg gttgaagttg ttcaactgtt ttttgagtat
    16921 aaacagtctt taagaacgta aaaaagcata agttcaggtc ttcaaaacct tgttcttatg
    16981 ctttttatca tagaaatatt tgctgaattt tctcttgaaa tgaaacctta tcttcatctc
    17041 gcgcttgtcc cttaccctgc ttttccgatt gcgagtgcgt agataaagaa gatggcgaag
    17101 ccaaagagga aaatcaatcc cgcaatggca aggtgtttga gccaagaagg aagatttttg
    17161 ttttcacgcg ttaccaaggc gtaattcaaa agagcaaaga aaggtgttgt caggaaagag
    17221 ccaatcatgg caaagcggag catggttgaa acctgaccag cgaagaactt gataatgacg
    17281 ataccgatga tagcagtgat ggtcatccag atgttcaaag atttacgatt gtcctctttt
    17341 tgactgatta gcagtcggag agattcctga ttaacgcgag aatagccatc gataacagtt
    17401 ataactgttc caaagataca gaggaaggca ataaaggtaa tcaagtaacg ggaccattcg
    17461 ccaagaacag aggcatacat acccacgaat tgagagatgt atttggctga agcagcttca
    17521 accgcctgcc ctgtaggata ctgaatcagt gctcccagtg ccacaaagaa gacggctagg
    17581 atagctgttc caatataacc agtgttaaag tcaaacagag cgtcttctgt gttaaagttg
    17641 acggtctttc tcttttcagc tgaccaaagt gaattgatag ctgaaatttc aataggagcc
    17701 ggcatccatc ctaggaggga gacgatgaag ggcagagctg ccatttgcca aggtgtcttc
    17761 tcgacaaatc agaactgtat tctggatgct tgaccgccgc aatgataact gcaagaacag
    17821 tcgcaatggt taaggcagac ataatccatt tgaccatgcc gtctaaaagt ttgtagcctc
    17881 caaagagtag catagcccaa atgattgcaa cgagaatgag ggaccactga gtaatgctaa
    17941 gtccaatcat tgggaaggca ctggcgatga tagctgagca cagaatggca acaccagccg
    18001 tgttgaccat agccgaaaag acattgagga taaagaaaat ccagagatag agttttcctt
    18061 tttcggcata accttcaacc aaagtctttc cagtatcagc tgtgtattca gcaccaaaac
    18121 ggaaaaatgg atatttaaag acattggcta agatgaccaa gagaagtaga gaccaaccgt
    18181 aagaaccgcc agcttgagtt gaggatacaa tgtgggaacc tccaacagcg gcagttgcca
    18241 ttaggattcc aggtcccatt gcttttagct tgcttgccca ggttgattga tttaaggaaa
    18301 tagcttgcga catgataaat actccttttt gaattttcag aataatctgt atatgtaatc
    18361 tatattctac aaaaaatttt ggaaaaatgc aagagtattt tgaaaaaaga tagaaaattt
    18421 acaaagaaga tctgaaataa ctgttatgat ataga
//
LOCUS       DAVKLW010000040        17474 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKLW010000040 DAVKLW010000000
VERSION     DAVKLW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.040"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            338..1384
                     /gene="pheS"
                     /locus_tag="VW324_001415"
     CDS             338..1384
                     /gene="pheS"
                     /locus_tag="VW324_001415"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9727042.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1384..1893
                     /locus_tag="VW324_001416"
     CDS             1384..1893
                     /locus_tag="VW324_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9727043.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1970..4372
                     /locus_tag="VW324_001417"
     CDS             1970..4372
                     /locus_tag="VW324_001417"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9727044.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4440..5441)
                     /locus_tag="VW324_001418"
     CDS             complement(4440..5441)
                     /locus_tag="VW324_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9727045.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5597..6043
                     /locus_tag="VW324_001419"
                     /pseudo
     CDS             5597..6043
                     /locus_tag="VW324_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6169..6435
                     /locus_tag="VW324_001420"
     CDS             6169..6435
                     /locus_tag="VW324_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9727046.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6696..8945
                     /gene="metE"
                     /locus_tag="VW324_001421"
     CDS             6696..8945
                     /gene="metE"
                     /locus_tag="VW324_001421"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9727047.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNSITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9009..9875
                     /gene="metF"
                     /locus_tag="VW324_001422"
     CDS             9009..9875
                     /gene="metF"
                     /locus_tag="VW324_001422"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9727048.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10560..12773
                     /gene="pnp"
                     /locus_tag="VW324_001423"
     CDS             10560..12773
                     /gene="pnp"
                     /locus_tag="VW324_001423"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9727049.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12789..13406
                     /gene="cysE"
                     /locus_tag="VW324_001424"
     CDS             12789..13406
                     /gene="cysE"
                     /locus_tag="VW324_001424"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9727050.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13418..14302
                     /locus_tag="VW324_001425"
     CDS             13418..14302
                     /locus_tag="VW324_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9727051.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14389..15732
                     /locus_tag="VW324_001426"
     CDS             14389..15732
                     /locus_tag="VW324_001426"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9727052.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15725..16111
                     /locus_tag="VW324_001427"
     CDS             15725..16111
                     /locus_tag="VW324_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9727053.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16115..16999
                     /locus_tag="VW324_001428"
     CDS             16115..16999
                     /locus_tag="VW324_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9727054.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(17046..>17474)
                     /locus_tag="VW324_001429"
     CDS             complement(17046..>17474)
                     /locus_tag="VW324_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9727055.1"
                     /translation="RKLSDKHFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLL
                     FHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKKKIVNALQLPYSNAKLEATNN
                     LIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         5193 a         3386 c         3990 g         4905 t
ORIGIN      
        1 tttgttttca tcttatttca atccgctata aagttagata agaataagct aacaagcaga
       61 agtaagatag gtcttgcaag aatggtaaaa atctgataaa ctaagtggta attgaataga
      121 aatctgaaag actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc
      181 tctctatatg aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact
      241 tgtcagatga aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg
      301 gttttctttt tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt
      361 aaaagcgctt cgcgaagaaa cgctgactag cttgaagcag attactgctg gaaatgaaaa
      421 agagatgcaa gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct
      481 caaagggatg aaagatgttt ctgctgagat gcgtccaatc atcgggaaac acgtcaatga
      541 agctcgtgat gtcttgacag ctgcttttga agaaacagct aagctcttgg aagaaaagaa
      601 agtcgcggct caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc
      661 gactggtcac cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg
      721 tatgggttat caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg
      781 tatgaacctt ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga
      841 agaaatcttg ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga
      901 tttttctaaa ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga
      961 cgatgcgacc cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat
     1021 ctctatggct gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga
     1081 gcgtcaaatt cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga
     1141 tgtttcttgc ttcaagtgtg gtggagaagg ctgtaacgta tgtaagaaaa caggttggat
     1201 cgaaattatg ggagccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc
     1261 gactgtatac tctggttttg cctttggtct tggacaagag cgtgtagcta tgctccgtta
     1321 tggaatcaac gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa
     1381 ataatgatta gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa
     1441 gcctttcgtg aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa
     1501 gaggcttata gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat
     1561 ttcattatgc atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa
     1621 actgagagag aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc
     1681 caaggatttg gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat
     1741 agtttttctt gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat
     1801 aatcgatatg gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat
     1861 acggattggt tactgagaaa gaaattaagg taagaagagg attcttctat tgaaatgata
     1921 ggaaaggaaa agaaccagtg tggcaatggt cattctggag aactaaatta tgcttatatc
     1981 ttataaatgg ttaaaagaat tggtggacat tgatgtgcca tcacaagagt tggctgaaaa
     2041 aatgtcaact acaggaatcg aggtagaggg tgtcgaatca ccagctgctg gtctctcaaa
     2101 aattgtcgtc ggtgaggtct tgtcttgcga agatgtgcca gagactcacc tccatgtttg
     2161 tcaggttaac gttggcgaag aagagcgtca gatcgtttgt ggtgccccaa atgtgcgtgc
     2221 tgggatcaag gtcatggtgg ctcttccagg agctcgtatc gctgataact acaaaatcaa
     2281 aaaaggaaaa atccgtggtt tggagtcact tggaatgatc tgttcacttg gtgaattggg
     2341 aatttctgac tcagttgtgc ctaaggaatt cgcagatggc atccaaatct tgcctgaaga
     2401 tgccgtgcca ggtgaggaag tcttttctta cctagacttg gatgatgaaa tcatcgaact
     2461 ttccatcaca ccaaaccgtg cagatgccct ttctatgtgt ggagtggctc acgaagtggc
     2521 agccatctat gacaaggcag tcaactttaa agaatttact ctaacagaaa ctaatgaagc
     2581 tgcggcagat gccctttctg tcagcattga gacagacaag gcgccttact atgcagctcg
     2641 tatcttggac aatgtgacca tcgcaccaag tccacaatgg ttgcaaaatc ttctcatgaa
     2701 cgaaggaatc cgtcccatca ataacgtagt ggacgtgacc aactacatcc tgctctattt
     2761 tggtcaacca atgcatgcct ttgacttgga taactttgaa gggactgaca tccgtgtgcg
     2821 tgaagcgcgt gctggtgaaa aattggtgac cttggacggt gaagaacgtg acttggacgt
     2881 gaatgaccta gtcatcactg tcgcagacaa gccagtagcc cttgcaggtg tcatgggtgg
     2941 tcaagcaaca gaaatctctg aaaaatctag tcgtgttgtc cttgaagctg ctgttttcaa
     3001 tggcaaatct atccgtaaga caagtggtcg cctgaacctt cgttctgagt catcttctcg
     3061 ctttgaaaaa ggaattaatg tggcaacagt taatgaagcc cttgatgcgg cagctagcct
     3121 gattgcggaa cttgcaggtg cgacggtgcg taagggcatc gtttcagcgg gtgagcttga
     3181 tacttcagat gtagaagttt cttcaaccct tgctgatgtt aatcgtgtcc tcggaactga
     3241 gctgtcttat gctgatgtag aagacgtctt ccgtcgtctt ggctttggtc tttctggaaa
     3301 tgcagacagc tttacagtca gagtcccacg tcgtcgttgg gatatcacaa tcgaagctga
     3361 cctctttgaa gaaattgctc gtatctatgg ttatgaccgc ttgccaacta gtctaccaaa
     3421 agacgatggt acagcaggtg aattgacagc cacacaaaaa ctccgccgtc aagttcgtac
     3481 tattgctgaa ggagcaggtt tgacagaaat catcacctat actctaacaa ctcctgaaaa
     3541 agcagttgag tttacggctc aaccaagtaa ccttacggaa ctcatgtggc caatgactgt
     3601 ggatcgttca gtcctccgtc aaaatatgat ttcaggtatc cttgataccg ttgcctacaa
     3661 cgtggctcgt aagaataaaa acttggccct ttacgagatt ggaaaagtct ttgaacaaac
     3721 aggtaatcca aaagaagaac ttccaaatga aatcaacagt tttgcctttg ccttgacagg
     3781 cttggttgct gaaaaagatt tccaaacagc agcagttcca gttgatttct tctatgctaa
     3841 gggaatcctt gaagccctat ttactcgttt gggactccaa gtaacctata cagcaacatc
     3901 tgaaatcgct agccttcatc caggtcgtac agccgtgatt tcattcggtg accaagttct
     3961 tggtttcctt ggccaagtgc atccagtcac tgccaaggct tacgatattc cagaaacgta
     4021 tgtggctgag cttaaccttt cagctatcga agctgcgctt cagccagcga ctccatttgt
     4081 agaaatcacc aaattcccgg cagtcagccg tgacgttgcc cttctcctca aggcagaagt
     4141 gactcatcaa gaagttgtag atgctatcca agctgccggc gtgaaacgtt tgacagatat
     4201 caaactcttt gacgtcttct caggtgagaa attgggactt ggtatgaagt caatggctta
     4261 tagcttgacc ttccaaaatc cagaagatag cttaacggac gaagaagtcg cacgctatat
     4321 ggaaaaaatc caagcatcgc tcgaagaaaa agtcaatgca gaagtgcgtt aaacgatagt
     4381 atgatacaaa ttccccctct agtttatgaa cactagaggg gtgattttat ataaaataat
     4441 taaaatgtga tttctgtaaa aatacatcta tgatcagagt tttgaaaact tacaatgtct
     4501 aaatctttaa catagtagtg gtttttaggc aataaaatat gatctattgt tgcattaaaa
     4561 agttttggac tttggctatt ccaagttcct ctttcaaaag gtggcagtac atttaatgcg
     4621 tccctatgag agcttatttt tgcaagtgct ccatgacgca tagttgcatt aaaatcacct
     4681 gcaataatag cctttggata ttttgaagcc aattgattat gaatgatgtt taagtcttgc
     4741 ttccagattt ccattaaacc tggcagagga ggagcagtat gcaaggcaat gatatctggt
     4801 atattttgtt ttctcgaatg taatacaatt gtcccgaacc gtgttgtatg aaaagtttta
     4861 gcttctgtat agaaaccata acttttcttg acaatcacag tcacaggagc tattccacta
     4921 ttggtaggtg gagaagtgaa aatatcatag ttggccatag aaagtccaac ttgatgaaac
     4981 aatagtttga ttctctggtt ttcttgctca cctctgatat tggtagctag ttcagggaat
     5041 atagccatat cggcgtcaaa atggctaaaa attcgctcaa tatgttgtgc ttctatttga
     5101 ttagcgacgt tccaagttac taattttaag gttttagtct tatttgatgc agaagaaagg
     5161 gttttatctg ttccgaataa tagtaatatg agcgatagta agagcaaaat gccagagtaa
     5221 aaccgtattt gtttattttt cttgtaaaaa gatagggaaa aaaatacgaa acttaaaata
     5281 gcagccatag ttgcagtagc taaggggaag gcataaatct gtgccaagat aggttgggta
     5341 gatagccaaa tgttaagatt agaagatggg aaaaaagaat aagttataat agggatagaa
     5401 aagagaatga ccgaaagggc agagagaaat gttttaatca ttgtgtgagt acctcaattt
     5461 gcaatggttg ctcggagcta atgtgacttt gtgactttac ttgattgata ttctttttct
     5521 tgtcttcctc atagattaag tacaacccaa gtaaaaaaag tccaatttaa gattgaaatg
     5581 attaaaaggc acagaaatgc aagacaatta tactacaaaa ggcaaacatt tgacaatcga
     5641 tagccgtcgc ttaatcgaac gatggaaaaa agaaggaaaa tcaaatagag aaattgcctc
     5701 tctacttgga aaagctcctc aaactatcca tactgaaatc aagtatggga cagtccgaca
     5761 atgtcttgga aaagggcgct tcaaagagat ttattctgcc gactacgctc aacagtctta
     5821 tgaaaataat cgcaagcact cggtcaagaa atcaagcttg actaagaaac taaaggaaaa
     5881 gattctccac tatcataacc aaaaattttc gcctgaaatg atggtgatgg ctaaaggggt
     5941 taacgtggga atttcaacca tttactattg gattcatcgt ggaaaattgg ggttaagcaa
     6001 gcaggatctg ctttatccta gaaaaggaaa agcgcttaag aaataggcta gcaccaactt
     6061 taaacccgct ggtcaatcta tcgaatcata attcgctcat ttttttaaaa atacatagac
     6121 gtaaaaaaaa gagggttaag tgatgaagaa tagtgctatt gggagtaatt ggaaggatgt
     6181 ccatctgagc tctttagcaa ggaggaaatc cttgaaagtg atatgcgagt ggctatcatg
     6241 agcgagttga ttgaggctag gtatgaacaa ggaatcagtc agaaaaagct ggaagaagtc
     6301 agtggaataa gccagcctgt catagctagg atggagacag gagagagcag tcttcagttg
     6361 gatacggtct taaaagttct aaccagtcta ggaaagacac tagcagtcgt tccacttgaa
     6421 caggggaaaa gttgactaga atcagttata gtcttttcta ataacaggtc atagtcactt
     6481 ataagaatta ctaataacat gttttagtat tctaattgga atatagctgt aaagtaagaa
     6541 ttacaaagga tttgagacgt ttgcttcaag tctttttctt ttgtccgaaa cagatatata
     6601 gtttcaaact atatcaaacc ctaatttttt acaattatac agacgctttc ttttctgtta
     6661 agatagtttc aacaacaaat tttggaggac acatcatgtc aactacaatc atcggtttcc
     6721 ctcgtttggg cgaattccgt gaattaaaat ttacaactga aaaatacttt agaaaagaaa
     6781 tctcagaaga agaactttta gcagcagcaa aagacttgcg tgctaaacac tggaacattg
     6841 tcaaagaaaa aggcatcact gaaattccat caaatgactt ttctcactat gataacttcc
     6901 tagatgcggc tttccttttc aacgtggtgc ctgcatccgt tcaaaacttg gacttgtctg
     6961 accttgagcg ctacttcgct ttgggacgtg gttaccaggg agaaaaaggg gacgttcgcg
     7021 cccttccaat gaagaaatgg ttcaacacta actaccacta catcgttcct aaatttgaaa
     7081 aagacactca agtaaaattg gctggtcaca agattttcga tgagttccaa gaagcaaaag
     7141 aacttggatt gaacactcgt cctgttcttg taggtccatt cactttcctt caattgtcag
     7201 actttgaaga aggcgtgaaa gcagacgact tcgtagatag cttagtggtt gcttaccaag
     7261 aagtttttgc taaattggct gaacttggtg cgactcgcat ccaattggat gaagcggctc
     7321 ttgtaaaaga cttgacagct gaagaaaaag ctctcttctt gaacctttac aacaaactct
     7381 tggctgacaa aaaaggtctt gaagtcttgc ttcaaactta tttcggtgat gttcgtgacg
     7441 tatacgctga ccttgtaaac ttgccagtag atgctatcgg tcttgacttt gttgaaggta
     7501 agaaaactct tgaactcgtt aaaggtggct tcccagctga caagactctc tatgtaggta
     7561 ttgtcaatgg taaaaacatc tggcgcaaca actacgaaaa gagcttggct gttcttgagc
     7621 aaatcccagc tgaaaacatc gttttgacaa gctcttgctc acttcttcat gtgccattta
     7681 caactgctaa tgaagaattt gaaccagcac tcttgaacca ctttgccttt gcagttgaaa
     7741 aattggatga aatccgtgac ttggatgcta tccgcaatgg tcaaggttca gaagctcttg
     7801 cagccaacaa agaactcttt gcgactgagc gtgttggtga aaatgctgaa cttcgtgcgc
     7861 gtatcgctgg cttgacagac gcagactaca ctcgtttgcc agcctttgca gaacgtgaag
     7921 ctatccaaga agaagctttc aaacttccag ctcttccaac aacaacgatt ggttcattcc
     7981 ctcaaacaaa agaagttcgt gccaaacgtt tggcttaccg taaaggtgaa ttgtctcaaa
     8041 aagagtacga tgctttcctt gctgaaacga ttgacgaatg gatcaagtgg caagaagata
     8101 ttgactttga tgtccttgtt cacggtgaat ttgagcgtaa tgacatggtt gagtacttcg
     8161 gtcaaaactt gtcaggatac ctcttctcta aaaatggatg ggtacaatca tacggtatgc
     8221 gtggggtaaa accaccaatc atctggggtg atgtcactcg tcttaactct atcactgtta
     8281 aatggtctag ctatgcacaa agtcgtacaa acaaacctgt taaaggtatg ttgactggac
     8341 ctgttaccat ccttaactgg tcattcccac gtgaagacat ctctatcaag gattcaactc
     8401 ttcaaatcgc ccttgctatc aaggatgaag tacttgacct tgaagctgct ggtgtgaaaa
     8461 tcatccaaat cgacgaggct gctcttcgtg aaaaattgcc actccgccgt agcgactggt
     8521 acgaagacta ccttgactgg gctattcctg ccttccgctt ggtacactca acagtagcgc
     8581 cagacacaca aatccacact cacatgtgtt actcagaatt tacagatatc atcccagcta
     8641 tcgataacat ggatgcagac gttatttcct ttgaagctag ccgttcaaac cttgaaatct
     8701 tggacgaact caaagcgaaa aacttccaaa cagaagtggg acctggggtt tacgatatcc
     8761 actcacctcg tgtaccaaat gaaggcgaaa tcgacaacac aatcgaagcc atccttgcta
     8821 aagtgccaag caagaaagtt tggatcaacc ctgactgtgg tttgaaaaca cgtggtatcc
     8881 cagaaacaaa agaaagcttg atccgccttg ttgaagcagc taaagctgcg cgtgagaaat
     8941 tgtaagattg gatttctctc catacagtgg ttgttcaaaa agaaatcaac tagaaaaggt
     9001 tattacatat gtcacgccaa acaccgtcac tctcatttga agtgttccct ccaaacccag
     9061 ccgtgggtaa tggtaacatt atttcagcct tgcaggatat gcaggagttg gctccccatt
     9121 ttatcagtgt aactgccagc aataataaat ttaatatcaa ggaaacgacg gttcgtttgg
     9181 ctgactttat ccaaaatgac ttggcgattc cgaccattgc ccacttgcca gctatttatc
     9241 taactaagga caaggttgct gaaactattg ctgacttgga caaagttggg gtgcagaaaa
     9301 tcttggctct tcgtggggat attattccag acgtagaacc acaaaaggat ttccgctatg
     9361 caaccgactt gattgagttt atcaaggaac aagcccctca ctttgatatt attggagctt
     9421 gctatccaga agggcatcca gattcgccaa atcagatttc agatattcaa aatcttaaga
     9481 agaaagtgga tgcaggctgt tcgagcctcg taactcagct cttctttgat aatgagcgct
     9541 tctatgattt tcaggacaag tgtattttgg ctgggattga tgttcccatt catgcaggaa
     9601 ttatgccaat tctgaatcga aatcaggctc tccgactctt gaagacttgt gagaatatcc
     9661 atcttccacg caaatttaaa gccatcttag acaagtatga gcatgaccct gagtcgctca
     9721 gagcagcagg acttgcctat gcagtggacc aaatcgtgga cttggtaact caggatgttg
     9781 ccggtgtgca tctctatacg atgaataatg ctgatacagc aaaatacatc catcaagcaa
     9841 cccatgcctt gtttaatcac cagtctctag gataataaaa gcaaaccatt cttctcaggt
     9901 gaggggaatg gttccttttt aatggtaaag acctcacttt ttaagaaaaa tatgataaaa
     9961 taaattctgt acgtacttga tacaaagatg aaggtattaa ataatacaat gcatttaaac
    10021 tgtaagattt aacgacacga gtgagtcaaa tcgatgttat ttgacgagag agttagtaaa
    10081 gcatttagct aatcgcccga tagcgattag cgtgaaagct ttagatactt tagtattaaa
    10141 cctttctaat gatgtatctt gaaaactgaa cttgcctaca actctgtgga aaaagataaa
    10201 tctccctagg agcagtcgct ccgtcgttcg atttcctatt ttcctttgag ttgcttgacg
    10261 gcttggtatc ttgatgactt gaattttggt ctcatttctt aggaaaaaag ataagcttcc
    10321 tagcactcgt cgagtgcgac gtcaccttcc tatttttcta cagaaatgtt cggcaagccg
    10381 aaccgtccaa aatatcttgt gtaattgaac acggccgaaa agctgtgtaa aaaagataaa
    10441 ctgtcttgtc ttcatcgaag acttcgtcag tttcctattt ttactttgct tttgacgtcc
    10501 ttggtatctt gatctttgta ggcaaggcgt ataatttcat caatccaaag gggattaaaa
    10561 tggcaaaaca agtgtttcaa acgacttttg cgggtcgtga gttaattgta gaaactggtc
    10621 aggttgctaa gcaagcaaat ggctctgttg tcgtacgtta cggtgagtca actgtcttga
    10681 ctgctgccgt tatgtctaag aaaatggcaa ctggggattt cttcccactc caagtcaact
    10741 acgaagaaaa aatgtatgcg gctgggaagt ttcctggtgg ctttatgaaa cgtgaaggac
    10801 gtccttcaac agatgcgacc ttgacagcgc gtttgattga ccgtccgatt cgtcctatgt
    10861 ttgcggaagg tttccgtaat gaagtccaag tcatcaatac agtgctttct tatgatgaaa
    10921 atgcatctgc accaatggct gctatgtttg gttcatcttt ggcactgtct atttcagata
    10981 ttccatttga cggaccaatt gctggggtac aagtgggata tgtagatggc caaatcatca
    11041 tcaacccaag tcaagaacaa gcagagcaat ctcttcttga attgacagta gctggaacca
    11101 agcacgctat caacatggta gagtctggtg ccaaagaatt gtcagaagaa atcatgttgg
    11161 aagcgcttct taaagggcac gaagctgtca aagaattgat tgccttccaa gaagaaatcg
    11221 ttgctgctgt cggtaaagaa aaagcagaag tggaattgct tcacgtggat gctgaattgc
    11281 aagctgaaat cattgcagcc tacaacagtg acctccaaaa ggcagttcaa gtagaagaga
    11341 aattggcccg tgaagctgca actcaagcag tgaaagacca agtgactgcc gtttacgaag
    11401 aaaaatatgc gaaccacgaa gaatttgacc gtattatgcg tgatgtggct gaaatcttgg
    11461 aacaaatgga acacgcagaa gtgcgacgtt taattacaga agacaaggtg cgtcctgatg
    11521 gtcgtaaggt cgatgaaatc cgtcctttgg atgcggttgt tgacttcctt cctcgtgtac
    11581 atggttcagg tctctttact cgtgggcaaa ctcaagctct ttcagtcttg accttggctc
    11641 cgatgggaga aactcaaatc attgatggtt tggatccaga gtacaagaaa cgctttatgc
    11701 accactataa cttccctcaa tattctgtag gggaaacagg tcgttacggt gcgccaggtc
    11761 gtcgtgaaat cggtcacggt gcccttggtg agcgtgctct tgctcaagtc ttgccaagct
    11821 tggaagaatt cccatacgct atccgtctag tagcagaagt tttggaatca aacggttctt
    11881 catctcaagc ttctatctgt gcgggaactc ttgcccttat gactggtggt gtgccaatca
    11941 aggcgccagt agctggtatt gctatgggac ttatctcaga tggaaataac tacacagtat
    12001 tgacagatat ccaaggtttg gaagatcact ttggagatat ggacttcaag gttgcaggta
    12061 ctcgtgatgg gattacagcc cttcaaatgg atatcaagat tcaagggatt actgcagaaa
    12121 tcttgacgga ggctcttgct caagccaaga aagcgcgttt tgaaatcctt gatgtcattg
    12181 aagcaaccat tccagaagtt cgtccagaat tggctccaac tgctccgaaa attgatacga
    12241 tcaagattga tgtggacaag attaagattg tcatcggtaa gggtggagaa accatcgaca
    12301 agattatcgc tgaaacaggt gttaagattg atatagacga agaaggaaat gtgtctatct
    12361 actctagtga ccaagatgct attaaccgtg ccaaagaaat tattgctggt ttggttcgtg
    12421 aagccaaagt ggatgaagtt taccgtgcta aagtcgttcg tatcgagaaa tttggtgcct
    12481 ttgttaacct ctttgataag acagatgccc ttgttcatat ctctgagatg gcttggactc
    12541 gtaccaatcg tgtagaggat ttggtagaaa tcggggatga agttgatgtt aaggttatca
    12601 agattgatga aaaaggccgt atcgatgcct ctatgaaggc tcttctacct cgtccgccaa
    12661 aacctgagca tgatgaaaaa ggtgaaaagt ctgagcgccc tcaccgccca cgtcatcaca
    12721 aggatcacaa acctaagaaa gaatttacag aaacaccaaa agattcagag taagaaaagg
    12781 agaaatgtat ggggtggtgg cacgaaacca ttgatatcgt aaaagaaaat gatccagcgg
    12841 cccgcaccac tttggaggtt ttgctgactt atccaggtgt caaggccttg gcggcccacc
    12901 gtctctcgca ttttctctgg aagtacgact tcaaactctt agctcgtatg cacagtcagt
    12961 tttggcgctt ttggactcag attgagattc atccaggagc ccagattgat tcaggtgtct
    13021 ttattgacca tggttctggt ctggtgattg gagagacagc gattgttgaa aaaggcgttc
    13081 ttctctatca cggagtgact ctcgggggaa cagggaaaga ctgtggcaaa cgccatccga
    13141 ctgtacgaaa gggagccctc atatcagccc atgcccaagt tatcgggcct gtggaaattg
    13201 gtgaaaatgc caaggtcggt gctgcagcag ttgtcgtagc agatgtacct agtgatgtga
    13261 cggttgtcgg tattccggcc aagattgtcc gtcttcatgg taagaaagat gagcctgtta
    13321 ttcacgaagt cgaagaaaaa cgggagtatt atgtcaataa actcgagcag gctaaagatg
    13381 ccagtcacag atcgtctggt ttgtagagga tacctatatg attcaagcaa gaaacaagtt
    13441 aagccaagag gagctatctg aggcgaaaaa agtaattaac tgttgccaaa actatgacgg
    13501 tacctatcgc gatccctatc tctctaacat gcttaatttt gacccaaaca tgcccgcctt
    13561 tttcctttat tatgaaaaag gcaaacttgt tggtttatta actgtctatg cagatgacca
    13621 agatgtagaa gtgacgatat tggttcatcc aggtcatcgc cgtcagggga ttgcgcgtgc
    13681 attgtttact agttttgaga gagaaacagc ttctttcccc attcggtcag tcacttttca
    13741 gacagaacgt atttttttag agaatcatcc tgactttgcc agtaactggg gactaatcga
    13801 ggatgaagag acagaaactt ggttaggtaa ggatagaaga ccttatccgt tagcaaatgt
    13861 ttcccatctt gaagttttgt tagcagatag ttcgtatcag gatcaaatta gtcaattaaa
    13921 atttcaggca ttttcagagg aacatgaatc gagagaagtt gtggatagat atgtcgctga
    13981 agctctgaag gatccagaaa gtcgcttata tattttgtta aaagacggtc aggttattgg
    14041 gacttgcacg gtagatttat cgactaatac gaattacttc tacggtttag caatatcgga
    14101 acctgaacgt gggaaaggct atggaagcta cttagcaaaa tccctcgtca accaactaat
    14161 tgagcaaaat gacaaggaat ttcagattgc agtggaagat agcaatgtag gtgccaaacg
    14221 tttgtatgaa aaaattggct ttgtcaaaca gacacaggtg gtttatctga atgagaaagg
    14281 agcaagggat tccgaagtgt agagacattc ggactgaaat ttacttgagc tcttagttta
    14341 gtgatgaaac taacagttct tggattttag ctttcctgat tatgatttat gattaaaatc
    14401 tatgacacca tgtctcgtga tttgcgagaa tttgtcccga ttgaggacgg caagatcaag
    14461 atgtatgttt gtgggccaac cgtgtacaac tatattcacg tgggaaacgc ccgttcgacg
    14521 gtagcttttg atacgattcg tcgctatttt gagtaccgtg ggtataaggt tgcctatatt
    14581 tccaatttta cagatgtgga tgataagatt atcaaccgtg ccagggaaga aggcatcaca
    14641 cctcaggagg ttgcggataa gtacatcgct gcctttcgtg aggatgtgac ggccttaggc
    14701 gtgaaacctg cgactcgcca tccgcgtgta gtggagttta tggcagacat catccgtttt
    14761 gtggaagact tgatcgagaa aggctttgcc tatgagagtc aaggggatgt ctatttccgt
    14821 gtagaaaaat cccacaacta tgctaaattg gctaataaaa ccttggaaga tttggagcta
    14881 ggtgcttcag gtcgtaccga tgaagaaacg gctcgtaagg aaaatcctgt agactttgct
    14941 ctatggaaat cttccaaacc aggtgagatt tcttgggata gtccttgggg acctggacgt
    15001 ccgggttggc atattgagtg ttcagtcatg tcgacagaga ttttaggtga taccattgat
    15061 atccacggtg gtggagctga cctagagttt ccacaccaca ccaatgaaat tgcccaatct
    15121 gaagctaaaa caggcaaggc ttttgctaac tactggatgc acaatggctt tgtcaatatc
    15181 gacaatgtca aaatgtctaa gtccttgggt aacttcatta cggtacacga tgcccttaaa
    15241 actcttgatg ggcaagtact tcgtttcttc tttgcgaccc agcactaccg taagcctatc
    15301 aacttcacgg aaaaggcagt tcgcgatgcc gagaccaatc tcaagtatct gaagaatact
    15361 tacgaacaac catttacagg aaacgtggat gcccaagagt tacaaaactt taaagataag
    15421 tttgtagcag ctatggatga agattttaat gctgccaacg gtatcacagt tgtctttgaa
    15481 atggccaaat ggatcaactc agggaactat gatgcaagtg tcaagcaagc tcttgcagat
    15541 atgttagaaa tttttggaat tgtctttgtt gaggaagttt tggatgcaga gattgaagac
    15601 ttgattcaaa aacgccaaga ggcgcgtgcc aatcgtgact ttgagacagc agaccaaatc
    15661 cgtgaccaat tggttactca aggaattaag ctccttgata ccaaggatgg agtgaggtgg
    15721 acacgtgatt gatgtcaatc tcattaacgg gattgcgcta gcctttgagg gagatgcggt
    15781 gtattctatg tatattcgcc gccacctcat cctcaaaggt atgaccaagc ccaataaact
    15841 ccaccaagag gcgaccaagt acgtgtcagc caaggctcag gctcgtctga ttgctctcat
    15901 gttggaggag cagatcctaa cggaaaaaga agaagaaatc tacaaacgtg gccgcaatac
    15961 caatagtcac acaaaggcta aaaatgcaga tgtcgtgact tatcgtatgt ccacgggatt
    16021 tgaagcagtt atgggctatc tccatatgac tgagaatctg gaacgtcttg agagtttggt
    16081 ttcatggtgc atccaaaaag tggagggcta gaacatgagg gcaaaagaac tacaagactg
    16141 gtttcctgag gctcggattt cagaccaacc agtagagaaa gagggctatc tcacgcttcc
    16201 tttagcttct cagcagtgga ttttgctgga ggaagctggg ctcagcgagc gtgaaaagca
    16261 gttggttgcc cttttgaccc agcaggagca ggctcgttcg ctaaaccctt ggtattccta
    16321 tctggttgag ggcaagggac aggcaccgca agtttttaaa aagattcagt tggtttattg
    16381 ccatctttct tattttcagc aggaaaatct gacttcttgg ctagatatga tgcggactct
    16441 ttttccgaat tgtcagacag tgctacaggt cggagctcag gattatgttt tcgtgcttca
    16501 acaagacaaa tacacttctg taagagatat tttgagtgat acgattgaag cggttgagta
    16561 tgactttgga cttcgtcttt ctatcatgtt gggtcaggtt tggtctcaga caggacatca
    16621 agccctatca gacttaatca aagctgagcg ggatttgttc aagacatggt ggcgtcaggg
    16681 tcaccaaggt gttcatactt tttctcagct ctatctttgg agtatgggag aaagactcgt
    16741 ggacttgaag ccaattaaag aatgtctaca ccagatgatt ttggatcaag atcagattca
    16801 ggaaatcatt ctctctcttt gggaaaatag tgctgttctc actaaaacag cccagcaact
    16861 ctatctgcac cgcaattctc tccaatacaa gattgataaa tgggaagagt tgacagggct
    16921 tcagttgaaa gagttgaccg acctgacctt gtgttatcaa ttgattttag gttctttgtc
    16981 aactatagtt ggtttgtaaa gaagttaata tggctctttg tcaactgtag tgggttgaaa
    17041 aaaagctaag ctcgagaaag gacaaatttt gtcctttctt ttttgatatt cagagcgata
    17101 aaaatccgtt ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg tttgataagt
    17161 ttgatgagat tattggtcgc ttccaatttg gcgtttgaat agggtagttg aagggcgttg
    17221 acgattttct ttttgtcctt tagaaaggtt ttaaagacag tctgaaaaag aggatgaacc
    17281 tgcttaagat tgtcctcaat aagtccgaaa aatttctctg gttccttatt ctgaaagtga
    17341 aacagcaaga gttgatagag ctgatagtga tgtttcaagt cttgtgaata gctcaaaagc
    17401 ttgtctaaaa tctctttatt ggttaaatgc atacgaaaag tagggcgata aaaatgttta
    17461 tcgctgagtt tacg
//
LOCUS       DAVKLW010000041        16941 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKLW010000041 DAVKLW010000000
VERSION     DAVKLW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16941
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.041"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..238
                     /locus_tag="VW324_001430"
     CDS             <1..238
                     /locus_tag="VW324_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727056.1"
                     /translation="ICADTTIKFLFIRLFDTSTLMKRRIFFLFALDVIVYLFLGYLLV
                     IQKAGYLFSVLLLFSNFSVPFIKEKEYELFKNSK"
     gene            1364..1867
                     /locus_tag="VW324_001431"
     CDS             1364..1867
                     /locus_tag="VW324_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727057.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTISVIQTGVFILEDFRNILLYNDVIERISLYEFIGDIFYSKLTSYC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTN
                     VKEMKWL"
     gene            2153..4246
                     /gene="pspA"
                     /locus_tag="VW324_001432"
     CDS             2153..4246
                     /gene="pspA"
                     /locus_tag="VW324_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9727058.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKSEAAKKAYEEAKKKAEDAQKKYDEDQKKTEEKAENEKKAAADLNEATEVH
                     QKAYVRYFEIQRAKDSKKYKNNRDKYNKDLAEADQKIKDTKTVLDEKQSKFYAVRAVV
                     VPEAKELAVTKQKAEETKKGAEVAKEKYDKAAQEVEVAKKEVEAEEAELDKKVAELQN
                     KVADLEKEIADAEKTVADLEKEVAKLEKDVEGFKESDGEYAKFYLEAAEKDLATKKAK
                     LAEAKIKAATKKAELEPELEKAEAELENLLSTLDPEGKTQDELDKEAAEAELNKKVEA
                     LQNQVAELEEELSKLEDNLKDAETNNVEDYIKEGLEEAIATKQAELEKTQKELDAALN
                     ELGPDGDEEETPAPEAPAEQPKPEKPAEETPAPAPKPEKSADQQAEEDYARRSEEEYN
                     RLTQQQPPKAEKPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGS
                     WYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYN
                     DSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWAK
                     VNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVN
                     TTVDGYKVNANGEWV"
     gene            4647..5768
                     /gene="mnmA"
                     /locus_tag="VW324_001433"
     CDS             4647..5768
                     /gene="mnmA"
                     /locus_tag="VW324_001433"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9727059.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5909..6364
                     /locus_tag="VW324_001434"
     CDS             5909..6364
                     /locus_tag="VW324_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9727060.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            6374..8287
                     /gene="mnmG"
                     /locus_tag="VW324_001435"
     CDS             6374..8287
                     /gene="mnmG"
                     /locus_tag="VW324_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9727061.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMILPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQDKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            8476..8565
                     /locus_tag="VW324_001436"
                     /pseudo
     CDS             8476..8565
                     /locus_tag="VW324_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8666..10345)
                     /locus_tag="VW324_001437"
     CDS             complement(8666..10345)
                     /locus_tag="VW324_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9727062.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(10347..10580)
                     /locus_tag="VW324_001438"
     CDS             complement(10347..10580)
                     /locus_tag="VW324_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9727063.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(11398..11550)
                     /gene="cibB"
                     /locus_tag="VW324_001439"
     CDS             complement(11398..11550)
                     /gene="cibB"
                     /locus_tag="VW324_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9727064.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(11552..11737)
                     /gene="cibA"
                     /locus_tag="VW324_001440"
     CDS             complement(11552..11737)
                     /gene="cibA"
                     /locus_tag="VW324_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9727065.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11915..12598
                     /gene="tsaB"
                     /locus_tag="VW324_001441"
     CDS             11915..12598
                     /gene="tsaB"
                     /locus_tag="VW324_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9727066.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPR
                     QKEGLVVPIMDARRNNVYAGFYENAKPVLPEAHLSFAEVLEQVKDAEQVTFVGEVGAF
                     VEQIQEQLPQASYQETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            12595..13032
                     /gene="rimI"
                     /locus_tag="VW324_001442"
     CDS             12595..13032
                     /gene="rimI"
                     /locus_tag="VW324_001442"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9727067.1"
                     /translation="MIEIKRIQQQPDLAKAIYAVMIAVYPVSPWTLEQIQADLFQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGKGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIADRKAYYHDPVEDAIIMKREIDEG"
     gene            13022..14032
                     /gene="tsaD"
                     /locus_tag="VW324_001443"
     CDS             13022..14032
                     /gene="tsaD"
                     /locus_tag="VW324_001443"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9727068.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(14072..15378)
                     /locus_tag="VW324_001444"
                     /pseudo
     CDS             complement(14072..15378)
                     /locus_tag="VW324_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15654..16005)
                     /locus_tag="VW324_001445"
                     /pseudo
     CDS             complement(15654..16005)
                     /locus_tag="VW324_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16112..16405)
                     /locus_tag="VW324_001446"
                     /pseudo
     CDS             complement(16112..16405)
                     /locus_tag="VW324_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            16648..>16941
                     /locus_tag="VW324_001447"
     CDS             16648..>16941
                     /locus_tag="VW324_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU9727069.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVK"
BASE COUNT         5171 a         3151 c         3820 g         4799 t
ORIGIN      
        1 aatttgtgca gatactacga taaaattttt attcatcaga ttatttgaca caagtacttt
       61 aatgaagagg agaatatttt ttcttttcgc attggatgtt atagtttact tatttttagg
      121 atatctttta gtgattcaaa aagcaggcta tttgttttcg gttttacttc ttttttctaa
      181 tttttcagtt ccatttatca aagaaaagga atacgaatta tttaaaaata gcaagtaata
      241 gaaatatgga ttaaatctta gaacggctta aaatcagaca tgataaagga aagctatcta
      301 ggtaaccaag tactataaac acagtcgagg atcacttgtt aggattacat tttaatcatg
      361 cttaataaga atggagaatt cctatgggga ttgtattact ctgaagtttt gaatacctta
      421 gttctcgtga ttttgagcta ttctagcttt agaatccttc agaacgtaaa attttaagca
      481 gtcaaagact tagaatagcc cgaaaacatt tattttatag caattctaag attagaattg
      541 ctcatatata tgtatttgag ctgctcgata gttaggacag ctctattctg agaaatctga
      601 cctatcaatg cttcagaata gtatttcgca aatggttttt attgaagagg tgattgtttt
      661 atgatagtct aaaataaaac tgatttatgg agtaaatgga gaatcgtcac atgaaacatt
      721 tttttgcagg aattggtgag ataaaacttg ttggctactt attgtatata tgtgtaggac
      781 tagcccctct ttttcatata tatgtcatag gttcggaact gagttttgtg aaaatagtgt
      841 tatctatttt gggagttata tttgtaagta tgttaactat tgctcgaatt tatcggactt
      901 ttctataaag aatagtttgg acttgaactg tatctaaaaa cagaggttgg tcgattatca
      961 gcctctttct tgtcgttgaa aagagtgaga tatattgact tttgaaaaaa atgtcataat
     1021 tgagttggga taaacctaaa agtaatatag tagattgaat ctggaatagt ccgttatgac
     1081 tgttaaaata tttctagaaa ttaatttgac tttcctaatc gatttgttca catcttgttt
     1141 caatctacta tactttctcg ttaaaccgtt cggcaattca tggtgacatg gacacatagt
     1201 agtaagcaca ctatgtggct tggcagagct aaaaactgtt cccttgcgat aagcctaata
     1261 agcacaacat agggagttag agaagccgac tctaatcaat cactttgggc agtagtgaga
     1321 actgccccgt gctttttatt ttgagaaaat atggagtttg tcgttgaaat tacttgattg
     1381 tattttggat tatcaagaaa gattcaatgg aaaaacatgt caagtatcaa cgaattataa
     1441 gtatttagag actttcaaag tgaatttttg cttgactgat ttacatcatt tatttggctt
     1501 acacaaaatc acacgagatt ttgctagtca tacgatatct gttattcaaa ctggtgtttt
     1561 tattttggaa gattttagaa atatcctatt gtacaatgat gtaatcgaaa ggatatcttt
     1621 atatgagttt atcggcgata tcttctattc taagctaaca agttattgca tcgtagctaa
     1681 ggatttatct aaaaatacta tgaaattgga cgtcatattt tttgaggata aaaataaaag
     1741 atccgcagtt ttaggtttac gaagagataa aagcggagta tttaaaccag ttactctaca
     1801 ttttacaagc gctaagaaat atgctaaagt tcgtaaaaca aatgtgaaag aaatgaaatg
     1861 gctataaaga ttgtccgcag ccttaagctt gcgttttttg ttgtttaacg aaattaaaaa
     1921 acaagctctc tcatcggaaa tgtttttata ggaatgaagg aagatgatgc tatgtttaaa
     1981 aagagaatcg aaaagggcaa aagtagtgtc tttatctttt tggagtagaa gcttatgata
     2041 tagaaatttg taataaaaat gtaatataaa acatttgaca aatatttacg gggggagtat
     2101 acttaatata agtatagtct aaaaatgact atcagaaaag aggtaaattt agatgaataa
     2161 gaaaaaaatg attttaacaa gtctagccag cgtcgctatc ttaggggctg gttttgttac
     2221 gtctcagcct acttttgtaa gagcagaaga agcccccgta gctagtcagt ctaaagctga
     2281 gaaagactat gatgcagcag tgaaaaaatc tgaagctgct aagaaggctt acgaagaagc
     2341 taaaaagaaa gcagaagacg ctcagaaaaa atatgatgag gatcagaaga aaactgaaga
     2401 gaaagccgaa aatgaaaaaa aggcagcagc agacttgaac gaagccacag aagtgcatca
     2461 aaaagcatat gtaaggtatt ttgaaattca acgagcaaaa gattcaaaaa aatataaaaa
     2521 taatcgtgat aagtacaata aagatctcgc tgaagcagat caaaaaataa aggatacaaa
     2581 aacagtatta gatgaaaagc aatctaaatt ttatgctgta agagcagttg tagttcctga
     2641 agcaaaggag ttagctgtta ctaaacaaaa agcggaagaa actaaaaaag gcgccgaagt
     2701 agctaaggag aaatatgata aggcagctca agaggtagaa gtagcgaaga aagaagtaga
     2761 ggcagaagaa gctgagttgg ataaaaaagt tgctgaactt caaaataaag ttgctgattt
     2821 agaaaaagaa atagctgatg ctgaaaaaac ggtagcagat ttagaaaaag aagttgctaa
     2881 acttgaaaaa gatgtagaag gtttcaaaga atcagatggt gaatatgcca aattctacct
     2941 tgaagctgct gaaaaagacc tagctactaa aaaagctaaa ttagcagaag ctaaaattaa
     3001 agctgctact aaaaaagctg aattagaacc tgaattagaa aaagcagaag ctgaacttga
     3061 aaacctatta tctacactag accctgaagg taagactcag gatgaattag ataaagaagc
     3121 tgctgaagct gagttgaata aaaaagttga agctcttcaa aaccaagttg ctgaattaga
     3181 agaagaactt tcaaaacttg aagataatct taaagatgct gaaacaaaca acgttgaaga
     3241 ctacattaaa gaaggtttag aagaagctat cgcgactaaa caagctgagt tagaaaaaac
     3301 tcaaaaagag ttagatgcag ctcttaatga gttaggccct gatggagatg aagaagaaac
     3361 tccagcccca gaagcaccag ctgaacaacc aaaaccagaa aaaccagctg aagaaactcc
     3421 agctcctgca ccaaaaccag agaagtcagc agatcaacaa gctgaagaag actatgctcg
     3481 tagatcagaa gaagaatata accgcttgac tcaacagcaa ccgccaaaag cagaaaaacc
     3541 agctccagct cctgcaccaa aaccagagca accagctccc gcaccaaaaa cgggctggaa
     3601 acaagaaaac ggtatgtggt acttctacaa tactgatggt tcaatggcta caggttggct
     3661 acaaaacaac ggttcatggt actacctcaa cagcaatggt gctatggcta caggatggct
     3721 ccaatacaat ggttcatggt attacctaaa cgctaacggc gctatggcaa caggttgggc
     3781 taaagtcaac ggttcatggt actacctaaa cgctaatggt gctatggcta caggatggct
     3841 ccaatacaac gattcatggt attacctaaa cgctaacggc gctatggcaa caggttgggc
     3901 taaagtcaat ggttcatggt actacctcaa cgctaatggt gctatggcaa caggttgggc
     3961 taaagtcaac ggttcatggt actacctaaa cgctaacggt gctatggcaa caggttgggc
     4021 taaagtcaac ggttcatggt actacctcaa cgctaacggt tcaatggcaa caggttgggt
     4081 gaaagatgga gatacctggt actatcttga agcatcaggt gctatgaaag caagccaatg
     4141 gttcaaagta tcagataaat ggtactatgt caatggttta ggtgcccttg cagtcaacac
     4201 aactgtagat ggctataaag tcaatgccaa tggtgaatgg gtttaagccg attaaattaa
     4261 atcatgttaa gaacatttga cattttaatt ttgaaacaaa gataaggttc gattgaatag
     4321 atttatgttc gtattcttta ggtacctatc ttatgatttc aggaaatgtc attaaaaaaa
     4381 cgactccttt tctctaactt gaaaaataga ttagagaaaa tgggttgttt tatttattat
     4441 agttatttga atgaagataa gaagaaggta tactcacatc attcacataa tctgtatatt
     4501 gactataagt tttaaaaaac aatttttaag ctcttccttg tcttctctaa ccaagcgtgt
     4561 tataatgaat actgctcaag cgaccttcaa tcgtgaagca cacacgacct tcaatcgtga
     4621 ataaacgaat agatgggaga cttaccatga gtgataactc taaaacacgt gttgtcgtgg
     4681 ggatgagtgg tggtgttgat tcgtcggtga cggctctttt gctcaaggag cagggctacg
     4741 atgtgatcgg tatcttcatg aagaactggg atgacacaga tgaaaacggc gtctgtacgg
     4801 cgaccgaaga ttacaaggat gtggttgcgg tggcagacca gattggcatt ccctactact
     4861 ctgtcaattt tgaaaaagag tactgggacc gcgtttttga gtatttccta gcggaatacc
     4921 gtgcagggcg cacgccaaat ccggacgtta tgtgcaacaa ggaaatcaag ttcaaggcct
     4981 ttttggacta tgccatgacc ttgggggcag actatgtagc gactgggcac tatgctcgag
     5041 tggcgcgtga tgaggatggc actgttcaca tgcttcgtgg cgtggacaat ggcaaggacc
     5101 agacctattt cctcagccaa ctttcgcaag aacaactcca aaaaaccatg ttccctctgg
     5161 gacatttgga aaagcctgaa gtacgcagac tagcagaaga agcaggcctt tcgactgcta
     5221 agaagaaaga ctcgacaggg atttgcttta tcggagaaaa gaactttaaa aactttctca
     5281 gcaactacct gccagctcag cctggtcgca tgatgactgt ggatggtcgc gatatgggcg
     5341 agcatgcagg tcttatgtac tatacaatcg gtcagcgtgg cggactcggt atcggtgggc
     5401 aacacggcgg tgacaatgcc ccttggttcg ttgtcggaaa agatctaagc aagaatattc
     5461 tctatgtagg acaaggattc taccatgatt cgctcatgtc aactagccta gaagccagtc
     5521 aagtccactt tactcgtgaa atgccagaag agtttacgct agaatgtacg gctaaattcc
     5581 gttaccgtca gcctgactct aaggtgaccg tccatgtcaa aggagacaag gcagaagtta
     5641 tctttgcgga accacaacgc gcgattacac caggacaggc agttgtcttt tacgatggcg
     5701 aagagtgtct aggtggcggt ttgattgaca atgcttaccg cgatggacaa gtttgtcagt
     5761 acatttagat tgacaaattt tctcaatttg ctacaataat aacagcaata gaaatgatgg
     5821 tcaaagctca tggatgttgc aggctttttt gtcctgcact tctttgtagt tttgactgtt
     5881 tttgtgtcgt ttaagggaaa ggacaagaat gactcaacaa gactttcgga caaaagtaga
     5941 caatacggtt tttggtgttc gggcgacagc tttgattctt caaaatcgca agcttctagt
     6001 caccaaagac aagggcaagt attacattat cggcggtgcg attcaagtta acgaaaaaac
     6061 ggaagacgcg gtagtccgtg aagtgaagga agaactgggt gttaaatctc aagctgggca
     6121 gctagctttt gtggttgaaa atcgttttga agtggacggc gtttcctatc acaatatcga
     6181 gtttcattat ctggtggatt tgcttgaaga tgccccgttg accatgcagg aagatgagaa
     6241 aaggcagccc tgtgagtgga ttgacttgga taagcttgag gatatccagc tagttccagc
     6301 ctttttaaaa acggccctac cagattggga aggccaacta agacacattc atcttgagga
     6361 ataggagaga aacatgactt atcattttac tgaagaatac gatattattg taattggtgc
     6421 gggacacgct ggggttgagg cttccttggc cgctagccgt atgggctgta aggtcctgct
     6481 tgcgaccatc aatattgaaa tgctggcttt catgccttgt aatccctcta tcggtggttc
     6541 tgccaagggg attgtcgtgc gtgaagtcga tgccctcggt ggcgagatgg ccaaaaccat
     6601 tgacaagact tacatccaga tgaagatgct aaacacaggg aaggggccag ctgtccgtgc
     6661 ccttcgtgcg caggctgaca aggaacttta ctctaaggag atgcgcaaga cggttgaaaa
     6721 ccaagaaaat ctgacccttc gtcaaaccat gattgatgag attttggtgg aagatggcaa
     6781 ggttgtcggt gtgcgtacag ccacccatca agaatatgct gctaaggctg ttattgtgac
     6841 gacagggact gctctccgtg gggaaattat catcggagac ctcaagtact catcaggtcc
     6901 taaccacagc ttggcttcta ttaacctagc tgacaatctc aaggaactgg gcctcgaaat
     6961 cggtcgtttc aagacaggaa cccctccacg tgtcaaggct tcttctatca attacgatgt
     7021 gacagaaatc caaccaggag atgaagcacc taatcacttc tcatacactt cacgtgatga
     7081 ggattatgtc aaggaccaag tgccatgctg gttgacctac accaatggta ccagtcatga
     7141 gattatccaa aacaacctcc accgtgcgcc tatgtttaca ggtgtggtca agggagtggg
     7201 acctcgttac tgtccatcga ttgaggacaa gattgtgcgc tttgcggaca aggaacgtca
     7261 ccaactcttc cttgagccag aaggacgcaa tacagaggag gtctatgtcc aaggactttc
     7321 aaccagtctg cctgaggatg tccaacgtga cttggttcat tccatcaaag gtctggaaaa
     7381 tgcagagatg atgcgaacag gttatgctat tgagtatgac atgatcttgc ctcaccagtt
     7441 gcgtgcgact ttggaaacca agaaaatctc aggacttttc actgctggtc agacaaatgg
     7501 aacatcaggt tacgaagagg cagcaggcca agggattatc gcagggatca atgcggctct
     7561 gaaaatccaa gataagcctg agttgatttt gaagcgtagt gacggttaca ttggggtcat
     7621 gattgatgat ttggtgacca agggaaccat cgaaccctac cgtctcttga ccagtcgtgc
     7681 tgaataccgt ctcattctcc gtcatgacaa tgctgatatg cgcttgactg agatgggacg
     7741 cgagattggc cttgtggatg atgaacgctg ggctcgtttt gaaatcaaga aaaatcaatt
     7801 tgataatgag atgaaacgcc tagacagtat caaactcaag ccagtcaagg aaaccaatgc
     7861 taaggttgag gagatgggct tcaaaccctt gacagatgca gtgacagcca aggaattcct
     7921 tcgccgtcca gaagtttctt accaagatgt ggtagccttc atcggaccag ctgcagagga
     7981 tttggatgat aagattatcg aattgattga aacagaaatc aagtacgaag gctatatttc
     8041 aaaagccatg gatcaggttg ccaagatgaa acgtatggaa gaaaaacgca ttccagccaa
     8101 tattgactgg gatgacatcg attctattgc gacggaagct cgtcagaagt tcaaactcat
     8161 caatccagaa accatcggcc aagccagccg tatttcgggg gtaaacccag cagatatttc
     8221 tattttgatg gtgtatctgg aaggtaaaaa tcgtagtatt tctaaaactc ttcaaaaatc
     8281 aaaatgatac gtcgtcggct tcttacgaat gagttcaaag cttggctttg attcatctcc
     8341 agcctcccat agtttcccga actatgggag ctaactcaag ttatgcattt tcctagcctt
     8401 ctggtctgat tttcattgag ttttaatctc aaaagaaagc atagagaaaa acagctccga
     8461 agacggggct gttttgttga cttgtactca atgaaaatca aagagcaaac taggaagcta
     8521 gccgtaggtt gctcaaagca ctgctttgag gttgtagata gaactgacga agtcagctca
     8581 aaacaccgtt ttgaggttgt ggatagaact gacgaagtca gtaacatata tacggcaagg
     8641 cgaagctgac gtggtttgaa gagtattatt cttcatctgg tgtgaggatc atcgggatga
     8701 tgatcggttc acgttcggta ttttcataga ggaaggggcg aatagcgttg acaatggcac
     8761 cattgacaga ttgcacgcta gcatccttat ttttcagtgc gatacgaatg gcattgaaga
     8821 ggatacgctg gctttggcga atcaagtcgc cagactctct catgtagaca aagcctcggc
     8881 tgaggatgtc tggaccagat agaatcatct gcgatttgaa gtcaacagtt gcgactgcca
     8941 gaacgacacc gtcttcagat agatcgcgac gatctttgag gacagctgcg ccaatttcac
     9001 cgatacgatt tccatcgaca tagatatctt gggcgttgaa atgacctgcg atacgagctg
     9061 agtcagcagt aagggcaagc acatcgccat tgctcatgat aaagatattg tccttctcaa
     9121 caccagtatc cactgctagt ccagcgtgga ctttttgcat gcggtattca ccgtggacag
     9181 gcatgaagta ttttggctta atcaagcaga gcatgagttt ttgctcttgc tgaccaccgt
     9241 gtccagatgt atggatattg ttcactttac cgtggataac ttcgacacca gcttcagaaa
     9301 tgatgttaat cagcttgttg acgctagtag tgtttccagg gatgggacta gaagagaaga
     9361 taacggtatc acctggttgt aattgtactt gacggtgggt tccgttggcg atacgagaga
     9421 gggctgccat aggctcaccc tgactacctg tacagaggat aagaacttct cctgcaggat
     9481 aatctttgat ttcatttggc tcgataaagg ttcccttagg agctttgatg tagccaagat
     9541 cgattccgtt gacaatggcc ttttccatag aacgaccaaa gaccgcaatc ttgcgtccag
     9601 tcttaacagc agcttctgtt gcctgctgga gacggaagat atttgaggca aaggatgcaa
     9661 agatgatacg tccttcaata ccttggataa tcttcataat ggactgacca acgacttttt
     9721 cagagttggt aaaggttggt acttccgcat ttgtcgagtc agacaggaga cagagcacac
     9781 cttcttcacc aagcgcagcc atacgatgca agtccgcagg ttctccaact ggagtaaagt
     9841 cgaacttaaa gtcccccgta cagacgattt tcccttgagg agtatgaatg acaatcccca
     9901 aaggctctgg aatagagtga gtcgttctaa agaaagttgc cttgagattt ttaaaggtca
     9961 actcggtgtt gtggttgatt tcgtaaagtt tggcgttgcg caagaggccg tgttcttcga
    10021 gtttcccacg gatcaaagcc aaggcaagcg gtccagcgta gatagggaca tttgcttgct
    10081 tgagtaggaa cggaatccca ccaatgtggt cctcgtgtcc gtgtgtgatt aaaacagcct
    10141 tgacgcggtc gatattgtcc acgatgtaag agtagtcagg aatgacatag tcgataccaa
    10201 gcaagtcatc ttctgggaat ttaatcccag catcgacgat gataatctcg tcttggtatt
    10261 caattccgta agtgtttttc ccgatttctc ctagaccacc gatggcaaaa acgccgactt
    10321 cttcaggttt aagagtgtag gccatattag aactccgtaa tttcgaaggc gccagtttct
    10381 ttttcgtaat cgagcaattt gtcagacaag agttcgatat attcgatatt gtactctggg
    10441 cgattttctt cgacaagttg gcgagcagtg atacggccct caagttctga gctggcatcg
    10501 atgtctaggt aaagcgcgcg tgttgtttca cggcgtgggc tacgttcttt tgtttcttga
    10561 taaaaaactt tgtaaatcat atagttcctt tctatttaca ggtcagctta ttccaaactt
    10621 ttagcagata tttgcttgat ttcattccat tttgtgtcta gattgacctt gattcgttac
    10681 aattatttca attataccac aaaatgaaaa attagtaaaa gacggaaacg agaaagttag
    10741 ggggcaattt ctttggtaag cgtttacaaa aataaagaaa atgtattatg ttgataacgg
    10801 aagtgaaatt tattgcttgt cataattcca tatagatttt aagtcaagga ggttgcccta
    10861 tgtataactt atttgctttt gctatcggag ttcgtttgtt agcttttatc gtttttgttg
    10921 cttgcgttta tggaggaaat cttccatttg ccaaactgga acgatgacaa accaagttct
    10981 tgtggaaaat tacctttgtg actctctact cgctttttct cctcctcgtg ccctatgtcg
    11041 tttggtttgt attttacttt ggattaaatg cttagatgcc ctgcttatgc tggggttttt
    11101 tgtttgggga tgtagagata gtagatttac gacacgaaaa ttccacccca tctatcaaga
    11161 tgaggtggtc tttgcatttt tctaaaatga catgacaatg gaattttcaa agcttattat
    11221 ttttatggga gtcattgttc tgctcaaaaa tagagttgaa gcataaaaat aaaacaatac
    11281 aaacagataa atcgaatggt aaagggatag tagtaatgtt caaatcattg agaatgctga
    11341 gcaaactgta agagatgaag acaataaaat ttatccttct aaaaatgtgt ttcttatcta
    11401 gcatgactta ccaaactttt tacgaaggtc ttttccaatg gtataacctg ccataaataa
    11461 agcgatacca acacctacac caaagccacc cttgatttct tgcaattcct tattagaaat
    11521 ttcacgatag ttgttcaaat ttttcatcat attaatctcc tcctaaaaaa taaccagctg
    11581 ccaaaccaga acctataagt gctgttccac ctgcaatagc cttccaagat actgctactc
    11641 caaagataac caagggagcg agaccgccat caatatctga taattcattt tcagataagg
    11701 ataaaaattg attgtcaaga atgtcaaaat ttgtcattga actttcctcc ttttttcttg
    11761 ttcactttta tattcggaaa agaaaaggaa aagtgagcat ttttttaaat tatttttagt
    11821 taagtgaggg cactcttgtc tgggggcaaa gaaaattccc atgtgccagc ttttgtagta
    11881 taatagtaag cagacaaaaa gataggaatg tgttatgaaa gtattagctt ttgatacgtc
    11941 cagcaaggct ctttctctcg ctattttaga ggacaagcag gttcttgccg agacgacgat
    12001 taatattaag aaaaatcaca gtattactct tatgcctgcc atagattttt tgatggcaag
    12061 tttggattgg acacccaagg atttggaccg aatcgtggta gctgaagggc cgggtagcta
    12121 tacaggcttg cgaattgcgg tagcaactgc caagaccttg gctcataccc tgaacattga
    12181 gttagttggg atgtctagtc tattggctct ggtgccacgc caaaaagaag gcttggtggt
    12241 tcctatcatg gatgcgcgtc gcaacaatgt ctatgcaggc ttttatgaaa atgccaagcc
    12301 agtcttgcca gaagcgcatc tgtcttttgc ggaagtgcta gagcaggtca aggatgctga
    12361 gcaggtgacc tttgtcggag aagttggagc ctttgtggaa cagatccaag aacaactacc
    12421 acaagccagc taccaagaaa ccttgccaaa tgcagctaat ctagctcttt gggcttggga
    12481 caaagaagca gactccttgc acgactttgt gccaaattac ctcaagcgtg tcgaggctga
    12541 ggaaaactgg ctcaagaacc ataccgagtc tggcgagtct tacattaaac gcctatgata
    12601 gaaatcaaac gaatccaaca gcagcctgat ctagctaaag ccatctacgc tgttatgata
    12661 gctgtttacc cagtcagtcc ttggacgctg gaacaaatcc aagctgacct gttccaagac
    12721 cagacttggt atgctctggc ttatgatggg gcagaagtga ttggctttct agctgttcag
    12781 gagactctct ttgaagcaga agtcctgcaa atcgctgtca aaggagccta tcagggtaag
    12841 ggcattgcgt cagccttgtt tgctcaattg ccgacagata aggagatttt cctcgaagtc
    12901 agaaagtcaa atcaacgagc gcaagcattt tacaagaaag aaaaaatggc ggtcatcgct
    12961 gatcgaaagg cctactacca tgacccagtc gaggacgcca ttatcatgaa gagagaaata
    13021 gatgaaggat agatatattt tagcatttga gacatcctgt gatgagacca gtgtcgccgt
    13081 cttgaaaaac gacgatgagc tcttgtccaa tgtcattgct agtcaaattg agagtcacaa
    13141 acgttttggt ggcgtagtgc ccgaagtagc cagtcgtcac catgtcgagg tcattacagc
    13201 ctgtatcgag gaggcattgg cagaagcagg gattaccgaa gaggatgtga cagctgttgc
    13261 ggttacctac ggaccaggct tggtcggagc cttgctagtt ggtttgtcag ctgccaaggc
    13321 ctttgcttgg gctcacggac ttccactgat tcctgttaat cacatggctg ggcacctcat
    13381 ggcagctcag agtgtggagc ctttggagtt tcccttgcta gccctcttgg ttagcggtgg
    13441 gcacacagag ttggtctatg tttcggaggc aggagattac aagattgttg gggagacacg
    13501 agacgatgca gttggggagg cttatgacaa ggtcggtcgt gtcatgggct tgacctatcc
    13561 tgcaggtcgt gagattgacg agctggctca tcaggggcag gatatttatg atttcccccg
    13621 tgccatgatt aaggaagata atctggagtt ctccttctca ggtttgaaat ctgcctttat
    13681 caatcttcat cacaatgccg agcaaaaggg agaaagcctg tctacagaag atttgtgtgc
    13741 ttccttccaa gcagcagtta tggacattct catggcaaaa accaagaagg ctttggagaa
    13801 atatcctgtt aaaaccctag ttgtggcagg tggtgtggca gccaataaag gtctcagaga
    13861 acgcctagca gccgaaatca cagatgtcaa ggttatcatc ccccctctgc gactctgcgg
    13921 agacaatgca ggtatgattg cctatgccag cgtcagcgag tggaacaaag aaaacttcgc
    13981 aggctgggac ctcaatgcca aaccaagtct tgcctttgat accatggaat aaagagtggc
    14041 tctttgtcaa gtgtagtggg taggcgaaaa gctacaatct ggagagtacg aaattcgtac
    14101 tctctttttt gatgttcaaa gtcatcaaaa tccgtttttt aaaattgtta aagttccgga
    14161 aaccaaaggc tttgcgcttg ataaccttaa tcaaattatt ggtcgcttca agtttagcgt
    14221 tggaataagg cagcttaagt gcgttgataa tcttatctct atcccttaaa aaagtccaaa
    14281 agaccgtttg aaaaagcgga tgaaccgttg gcagttcttg ctcaatcaat ccaaagaaat
    14341 ggtcggcatt cttctcttga aaatgaaaga gcaggagttg atagagttcg tagtgaacct
    14401 gtaatccctc agagtaagaa agcagcttat ctcgtacttc ccgatgggtt aagtgcattc
    14461 gaaacgtggg ctgatagaat cgtttctgac agagcttgaa gctatcctgg tgtagtaaat
    14521 cagtccagtg gactgattta gcccgagcct agaaacgaaa aagcgagggt tccaaaagcg
    14581 cttgagcgcc cgatactcca aggattttcg gtcaaattgg ttcatgattt ggattcgtac
    14641 tcggttcata gctcggctca gatgttggac aatgtggaag cggtcaagaa caatcttcgc
    14701 ctttggaaag agttgcttag cgagccgata ataagggctg tacatgtcca tggtgatgac
    14761 ctcgaccagc tcccgaacct ctctctgata gcgttggaaa tggtttcgaa tcaccgcatg
    14821 agttcgcccg tctaaaattg cgaggatgga tttggactca aaatcttggg caatgaagct
    14881 cattttgctc tttttgaagc tatactcatc ccaactcata acttttggca acttggtcca
    14941 atcggtttca aacttaaatt ccctcagttt tcggatgacg gtggaagttg agaccgccaa
    15001 tctgtgggca atctcagtca tagactgctt ttcaaggagg agctgagcga ttttttgtct
    15061 caccatgttg gaaatctggc aatttttctt gacaatggat gtttgagaaa cgaccacctt
    15121 aaggcaattc ttgcactgaa agcggcgctt tttgagatgt agtaccgtgg gtaaaccctg
    15181 acagtcgaga agcggaattt tagaggcttt ctggaagtcg tatttgatca tcttcccttg
    15241 acaatgagga caaggaggag cgggggaatc caactttgcc tgaacgacta gatgggtttg
    15301 gtatttcaga acagacaaga ttttgatatt tttgtctttc aatccgagca aatctgtggt
    15361 attcttaagt tgttccatat gaaccctttc taatgagttg gttagacgct tttcattata
    15421 ggtcatatgg ggcttttttt ctacaagaaa cgaccctata attcctgggg tgggattacc
    15481 cactacagaa attatagagc caaaacattc caaagtcttg tctgactctg gaatgttttc
    15541 tgttcacagt tgttttgtaa ctgcctaata agtaaccgcg ctataacgag gtatctaccg
    15601 agattgtcaa ttctccagta tactgttata aaaaacaaaa ctggaggatt tttttatgtc
    15661 cccccatcgt cccatcgact gttccgtagc tgcccaataa cggagtatag tcggctgaaa
    15721 caagaatagg acaaaagtgc ctcaagaaaa gcatcgtaat ttgacaatat tattctgaga
    15781 tgtttttgat gtaagcccat attttctcaa tgggattata ctcgggtgaa taggtaggaa
    15841 gtggtaacag tctatgccct gctccttaca catgttcatt ctgtgaaacc ttgcattgtc
    15901 cataatgata agggatggtg tatctaaagt aggtagtaag aatttttgga agcaagcttc
    15961 gaaaaagtca ctcgtcatcg tatctttata ggtcatcgga gccatagtat attgaaacaa
    16021 aaaagggcat tagtacttct ataacagtat cgcaatctct ggtgtgcgtt gtctcgatct
    16081 taattcaaat aattatatct tcttccagag agactaactg cctgctcaag gctcgatcat
    16141 attcgagata aaaataggtc tcaacccctg tctcataaat ataaatagga gtcaggtagc
    16201 ttaagtgatt caattcttta aggaaccgat ttactttttc tgggtcttgt tcgcagtagg
    16261 tacagctctt ttttagacta tatcccatag cctttagagc gtaatgaata gttgttggag
    16321 gacagttgaa ttcagcagct atttcagtca aataagcatc gggatgagtt tcaatataga
    16381 tctttaattt atctctatca actttttcaa ttaatagtgg aagttttctt aaagttagtg
    16441 tgttgaaacg tttttataca gtgctaaaat ttgaagtgta gtgcagatgt atttgtaata
    16501 gtattatagc aattctttgg agaaggtcga gacaagagaa aataggtatc tttgagatat
    16561 aagcatgatt tactataaaa ttattgagtt attcataaaa atttctctat caaatatcat
    16621 aatgcgtcgt ttaaggagat ggttacaatg caatatagtt gtggaaaaat aaatattaat
    16681 atccctgatg gatatggaga cattaaggat atagtgtttt cggctcatat tatagttagg
    16741 tataataatg gacattgtgg aggtatagat ccgcatataa ttggactttg taaaaagcag
    16801 atcagaagga tgtctctata tcctattttg ataatcgtat ctagagattc aaaggttatt
    16861 gatgattata aaaatttaga tattgcctat gttgattgta ctcaatgttc aaataatttt
    16921 gaaactgcct tacacgttaa a
//
LOCUS       DAVKLW010000042        16686 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKLW010000042 DAVKLW010000000
VERSION     DAVKLW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.042"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            78..428
                     /locus_tag="VW324_001448"
     CDS             78..428
                     /locus_tag="VW324_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9727070.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            643..759
                     /locus_tag="VW324_001449"
                     /pseudo
     CDS             643..759
                     /locus_tag="VW324_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            752..985
                     /locus_tag="VW324_001450"
     CDS             752..985
                     /locus_tag="VW324_001450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727071.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEELL"
     gene            1112..1393
                     /locus_tag="VW324_001451"
                     /pseudo
     CDS             1112..1393
                     /locus_tag="VW324_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1527..2495)
                     /locus_tag="VW324_001452"
     CDS             complement(1527..2495)
                     /locus_tag="VW324_001452"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9727072.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2640..3423)
                     /locus_tag="VW324_001453"
                     /pseudo
     CDS             complement(2640..3423)
                     /locus_tag="VW324_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(3448..3945)
                     /locus_tag="VW324_001454"
     CDS             complement(3448..3945)
                     /locus_tag="VW324_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9727073.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            complement(4018..5379)
                     /gene="radA"
                     /locus_tag="VW324_001455"
     CDS             complement(4018..5379)
                     /gene="radA"
                     /locus_tag="VW324_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9727074.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(5393..5908)
                     /locus_tag="VW324_001456"
     CDS             complement(5393..5908)
                     /locus_tag="VW324_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9727075.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5910..6353)
                     /locus_tag="VW324_001457"
     CDS             complement(5910..6353)
                     /locus_tag="VW324_001457"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9727076.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            6658..6807
                     /locus_tag="VW324_001458"
     CDS             6658..6807
                     /locus_tag="VW324_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727077.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            6949..7128
                     /locus_tag="VW324_001459"
     CDS             6949..7128
                     /locus_tag="VW324_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727078.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(7348..7713)
                     /locus_tag="VW324_001460"
                     /pseudo
     CDS             complement(7348..7713)
                     /locus_tag="VW324_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            7733..7900
                     /locus_tag="VW324_001461"
     CDS             7733..7900
                     /locus_tag="VW324_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEU9727079.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            complement(8055..8258)
                     /locus_tag="VW324_001462"
     CDS             complement(8055..8258)
                     /locus_tag="VW324_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727080.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(8281..8472)
                     /locus_tag="VW324_001463"
     CDS             complement(8281..8472)
                     /locus_tag="VW324_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9727081.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            9044..9412
                     /locus_tag="VW324_001464"
     CDS             9044..9412
                     /locus_tag="VW324_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9727082.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            9412..9645
                     /locus_tag="VW324_001465"
     CDS             9412..9645
                     /locus_tag="VW324_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727083.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            9645..9908
                     /locus_tag="VW324_001466"
     CDS             9645..9908
                     /locus_tag="VW324_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9727084.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            9921..10297
                     /locus_tag="VW324_001467"
                     /pseudo
     CDS             9921..10297
                     /locus_tag="VW324_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            10314..11384
                     /locus_tag="VW324_001468"
     CDS             10314..11384
                     /locus_tag="VW324_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEU9727085.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            11439..12580
                     /locus_tag="VW324_001469"
                     /pseudo
     CDS             11439..12580
                     /locus_tag="VW324_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(12663..12756)
                     /gene="ffs"
                     /locus_tag="VW324_001470"
     ncRNA           complement(12663..12756)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW324_001470"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(12789..13256)
                     /locus_tag="VW324_001471"
     CDS             complement(12789..13256)
                     /locus_tag="VW324_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9727086.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(13457..14743)
                     /locus_tag="VW324_001472"
     CDS             complement(13457..14743)
                     /locus_tag="VW324_001472"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9727087.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(14974..15210)
                     /locus_tag="VW324_001473"
     CDS             complement(14974..15210)
                     /locus_tag="VW324_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9727088.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            15476..16273
                     /locus_tag="VW324_001474"
                     /pseudo
     CDS             15476..16273
                     /locus_tag="VW324_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16308..>16686)
                     /locus_tag="VW324_001475"
     CDS             complement(16308..>16686)
                     /locus_tag="VW324_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9727089.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEEMVTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIKK
                     NLKKVLPSCNTFYEALFSCSCFN"
BASE COUNT         5556 a         3661 c         2800 g         4669 t
ORIGIN      
        1 tctataaatt aatttgactt tcctgataga gttattcaca tcttatttca actcactata
       61 gaaggaggaa taggaggatt ctcagacatc cgggcatcag cccaactaat gatttgattg
      121 ctaagaaaat attcagcaat ccagaaatca cttgtcaatt tattcgcgat atgctggact
      181 tgccagcaaa aaatgtgacc attttggagg gaagcgatat tcacgtatta ctctccatgc
      241 cttactcggt gcaggatttt tataccagta tagacgtctt ggcggagttg gataacggta
      301 ctcaagtaat tattgagatt caagtccatc atcagaattt tttcatcaat cacttgtgga
      361 cttacctgtg cagtcaggtt aatcaaaatc ttgaaaaaat tcgtcagcga gaaggtgata
      421 ctcactagag ctacaaacac atcgctcctg tttacgccat tgctatcgtg gatagtaatt
      481 atttctcaga tgacctggct tttcatagct ttagtatgcg cgaagacaca acaggtgagg
      541 tattggcgat taccaacaat ggacaggaaa accatctggt taagatggca ttcttggaat
      601 taaaaaatac agagaaacca gcaaagacaa ggttcgcaag ccatggttgg agtttttcgg
      661 caacaagccc tttacccagc aaccgcaacg agccattagc caagcaaatc aactgctgga
      721 ctacaagagc tggtccgagg aggacaggaa aatgtttagt caactacata tgcgagaaga
      781 acaagtcttg ttagcacagg actatgcctt ggaaactgct agggctgaag gccttgaaca
      841 aggactagag cgtgggaaag ttgaaggaag ggcagaaagg aaactttttg ccttcctaga
      901 catagtacgc caaggtcttc tgacttctga ggttgccagc cagcaattag gtatgtcagt
      961 atctgaattt gaggaactgt tgtaaaatgg ctccataata tccatagtgg gtaaatcccc
     1021 tatggatatt atggagccta ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa
     1081 agcgacaaaa caactcatta gaaagaatca tatggaacaa ttacatttta tcacaaaatt
     1141 actagacatt aaagacccta atgtccagat tttaaacatc atcaataagg atacacacaa
     1201 ggaaatcatc gccaaactgg actacgacgc cccatcttgc cctgagtgcg gaaaccaatt
     1261 gaagaaatat gactttcaaa aaccttctaa aattccttat cttgaaacga ctggtatacc
     1321 tactagaatt ctccttagaa agcgtcgatt caagtgctat cactgttcaa aaatgatggt
     1381 cgctgaaact tctgatgacg tatagtcata tttcttctct ttttattata tcacagtttt
     1441 aaatctagct ttactagatt caccgctact atccatttat tcggaaaaaa gacgaaaaaa
     1501 cctgagaatc atctcaggct tggtcattaa atttttttct caatatcgaa aagtggagaa
     1561 agtggtcgtt tttcatgaat acgtacgata gcatccccta ggagatgagc gattgaaatc
     1621 tgctcaatct tatcaatcaa acgctcttct ggcagataga tggtatccaa aacaaccaat
     1681 ttcttaatag ctgatttttg gatattgtcc gtagcaggac cagaaagaac tgggtgcgta
     1741 cagcttgcat agactgcaac agcaccagct tccgcaagag catctgccgc atgacaaatc
     1801 gttccagcgg tatcaatcat atcatcaatc aagatacaag tcttgccttc aaccttaccg
     1861 atgatgttca taacttcact agtattcatc ttatcaacgc tacgacgttt atcaataata
     1921 gcgatagatg ttttcaaaaa ttctgccaac ttacgagcac gagtcacccc tccatggtcc
     1981 gggctgacaa ccacatagtc agaaccaacc ataccacgcc gctcaaaata atctgcaatt
     2041 agaggagcac ccatcaaatg atccacagga atatcaaaga atccttgaat ttgcgcagca
     2101 tgcaagtcga tggtcaataa acgatccact ccagctactt caagcatatt tgcgacaagt
     2161 tttgaagtga ttggctcacg cgctctcgcc tttctatcct gacgtgcata cccatagtaa
     2221 ggcatgacaa cattgacaga ctctgcactc gcacgcttca aagcatctac cataatcaaa
     2281 atttcaagca gattgtcatt tacaggcgaa ctagttgatt gtaagataaa gacgtgtttc
     2341 ccacggattg attcttcaat gttgacctga atctctccat ctgaaaattg gcgaacactt
     2401 gatttcccca actctatccc aatctcctgc gccacacgtt ctgccaattc tttattagaa
     2461 gaaagggcaa acagctttaa atcagaaaaa gacatgattt cctccggtat atatgtatcg
     2521 cttgtgcttt tcacaagatt ttccatctac cattgtagcg ctttttgcac tatttttcaa
     2581 tcaaaaataa aagaagggca ccatatttgt acccttgcat cattcttttg aaaaatattc
     2641 taggtcatca actcattgtg tttctcaaca aagcaataag catgataata accatagaga
     2701 gcaatagccg taaccactgg aatcgctaaa ggcaactctg tttccaactc cacaaaagga
     2761 gagttaaaca ataagaccct gtttcttgcc aaccttctgt cctttatagg ctctgtaaag
     2821 caagtaaaca cctactacag ctagacctga aaaagtccag tgagaggcaa ttcctgagat
     2881 gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat ccgtacgaat
     2941 ataaccaata tcctcaatca tttggaatcc caaaccggag gcaattccaa gtaaaaacaa
     3001 aaattttaat tttcgcacag gaatcaaagc caaaacaaaa acaagtggca ataatttcaa
     3061 gggttcttct accaaaggag ccacaatagc actttcaaag gcatttaaaa atggactatc
     3121 tgggaaaaga acccccagta aatcatggat ataagtatta gcaaaactag acaaccagcc
     3181 taaaaggaac atccctccca ataaagacag aatcaaaacc ttctttggca attcccattt
     3241 tttcccaata cggaagagaa aataaagagc cggaatcatg taaaagagag ctagaaagat
     3301 agaaactccc attagtccat attccgcacc tgacctcgaa ccgtccgtat agtagatggt
     3361 ttcatactgt aaaccaatac atagcaataa aataaaaata aataaaatac tgcttttctt
     3421 catacacttt ctttctaaat gaagtattta taattctacg actgccatac ttcctgtatc
     3481 aacattgtaa atggcaccag agataatgac atcgtctggt attaggggag actcgataag
     3541 cagttgcata tcctcgcgta cactctcttc tatatcttgg aagggcaaga agtcctggtc
     3601 tgacacatcg acacccaatt cttccttcaa atactcctga aaaggttcat tttcaaaggt
     3661 ctgagcacca cagtctgtat gatgcaatac cacaatttct cttgtcccca tttgttgctg
     3721 ggaaataact agagaacgaa tcatatcctc agtcactcga ccacctgcat tccgcaaaat
     3781 atgagcatcc ccaagtgcca aacctagagc ttgcgcaacg tgcaaacgtg agtccataca
     3841 ggtcacaatg gctactctgg ttttaggttt aagtggcaga tttaactgcc catgtagggc
     3901 aacataagcc tgattggctt gcataaactg ttcaaaatac gacacgattc cctccttgaa
     3961 aatttgatag tcaaatattt ctcctatctt atcattttta agagaatttg tcacggatta
     4021 tgcaaagacc tttttcaaga cttcctgaat cgttgtcacg ccaatgacct gaatttcctt
     4081 aggcagagtg attcctgtca aggaattctt aggtacataa atcttagtaa agcccagttt
     4141 agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc cgaatctctc ctgtcaagcc
     4201 cagttctccg acaaaacatt cctgaggatt agttggcttg tctttgtagc tcgaagcaat
     4261 agcaactgca acagccaagt caatcgcagg ttcatccaat ttaacaccac cagcagattt
     4321 gagataggca tcctgatttt gcaagagaag ccctgcccgt ttttccaaaa cagccataat
     4381 caagctagca cggttaaaat caagtcctgt cgtagtacgc ttggcatttc caaacatggt
     4441 cggtgttacc aaagcctgaa cctccgccaa aatcggacgc gtcccttcca tggttacaac
     4501 gatggaggaa ccagtcgccc catccaaacg ctcttctagg aaaacttgac tcggattgag
     4561 tacctcaacc aagccgcccg actgcatctc aaaaatccca atctcattag tggaaccaaa
     4621 acgatttttg accgctctca aaatacgaaa ggtgtggtga cgctcccctt caaagtaaag
     4681 caccgtatcc accatatgct ccaacatacg aggcccagcc aaggttcctt ctttggtcac
     4741 atgacctacg ataaagatgg caatgttatt ggtcttggcc aactgcatga gttcagcggt
     4801 cacttcacgt acctgagaaa cagacccctg cacccctgaa atctcaggag acatgatggt
     4861 ctggatggaa tcaataatga gaaagtctgg ctggatacgc tccacttctg cacgaacact
     4921 ctgcatattg gtctctgcat agagataaaa ctcactatca atatcaccta agcgctctgc
     4981 acgtagttta atctgctggg cagactcctc cccactgaca tagagaactg tccccacttg
     5041 ggacaactgg gttgagactt gtaggagaag agttgatttc ccaatcccag gatccccacc
     5101 gatgaggacg agacttcctg gtaccactcc gcctccaagc acacggttga attcctccat
     5161 ctccgtcttg gttcgattga cattgatgga agtcacctca gctagtttca tgggcttggt
     5221 tttctcacct gtcaaggaca cacgcgcatt cttgacctcg gcaacctcaa cctcttccac
     5281 aaaagaagac caagacccac agttggggca acgtcccaga tatttagggg aattataccc
     5341 acaattttga catacaaatg tcgctttttt ctttgcgatg acaaacctct ttctatatct
     5401 ctaactcaca ctcaatcact tggcaaaaat caatcttctc atttggcaca aactggcgca
     5461 tgagcattcg atgagcaaca actaccacag tctgatgttc tcgatactta gacatacatt
     5521 ctagaaaccg agacttcatt tccgtagctg tctcatattg aataggacta ttaggaagca
     5581 actccccctt gttttctaaa aacagtcttc tagctgtttc aaagttttct attcctgttt
     5641 tatagacctg ccattcatgt aataaaggct ctactcttaa aggaagaccc gtagcacaga
     5701 ccacatacga agccgtttct aaagctcttg tgactgcaga agatacgatt atttcagctg
     5761 acgagagtaa aggatttttg ctcaatttct ggacttgctg ccgtcccatc tcagacaagg
     5821 gtgccaaatc tatcccaaat cctatataag aacgctcctc taactcacgg taatctggct
     5881 ccccatgacg tacaaagata atcttcattc tagtgccctg tcgatccaaa tccaccagtt
     5941 cgaacgccat cagctgcatc tccatctgca attaagaaag tagcaaaaac agcctggaca
     6001 atacgttccc caacttcaag aacaacctct tgatctgtga tattcttcat ctgcgcaaaa
     6061 atatgccctt catttccagg atttccataa taatccccat caatgacccc aactgagtta
     6121 attaaaacca agcccttctt acgaggattt gaagaacgat catagaggta gagaacctca
     6181 gtcggctgca tataagcctt aacccctgtc ggaaccaaga caatctctcc tggcgcaaca
     6241 actgtacgca cagcaacctt taagtcgtaa ccagccgcat gcgctgtctc acgcttgggc
     6301 aataaatttt catctgtaaa actcgaaacc aattcaaaac cacgaatttt cataattttc
     6361 tcttttctat tatcatttat tctagattat tctatcttat ttattcggaa aaagcacgaa
     6421 aaaaagagca cacaacaatt ataggcgata cgataattta cactgtttca caatacgttg
     6481 aaatttagag ctttaaagcg agggcaaagt tgatttttta catcgtttta cagaggttta
     6541 cgacactttt gccccttttt tgcccctttg tttttcaaaa aaacttcatc aaatcgcttg
     6601 actttcacgg tttaccgtga tataatataa tcaagataag gaaaggaggt gaggaagttg
     6661 aacaaagaag attggcttag gttactggaa aaggcgatag ataatatccc tgaaacggta
     6721 actgctatag caagtctggt gactgcaata acggtcgcaa ggcaaaacaa aaagcgtaaa
     6781 ccgaaatccc gtcaaagaaa aaggtgaacg ctaagaggtt ggggcgaaag cccctcacac
     6841 ctctatttta tcaaatgaaa agaggaaaag caatggttag tgcaatagct atttttataa
     6901 ttgcggtcaa tgtatatatt tatctaaaaa ataaaaagga caaataagat gagagaagtt
     6961 attcaagaat tgttagacag ttcgatgtct acatctgcta tttcccaagg tgctggagtt
     7021 ccatggacta ctgtttctga cctcagaaaa ggaaaaacaa gcatggacaa aatggcactt
     7081 ctcacagcag aaaaacttta tgaatttgct acaactgata agcagtgatt tcggtcactg
     7141 tttttatttt tgacaaaaca ccgtttttga caataatcaa aaaataaaaa ggagctatta
     7201 ttaacaaaat ggcgtttttg gcaataatac cctgccattc ctcctcccta ttcttcaaga
     7261 aaacgctttt ttgaagaatc agagcaaata aaaaaccgca agcctgagcc tgcggtgaaa
     7321 gaacaattta gaaagtttcc tttctattta tttaactgta atcaagccat ctggctctac
     7381 tgtgaactct ggcttgtctg ccagtgttcc gtctggtttg aggtagtacc agcctgttcc
     7441 gtccgctgac tggataaagg catttgatac catggcgcct tctttagcgt ctaagtagta
     7501 ccaagtgtcc ttgtacttga cccagcctgt cttcatggca ccttctacat caaaatagta
     7561 ccacttgtca gcgattttct tccagcctgt ggccatttcg cctgattggt caaagtagta
     7621 ccaattaccg tctgtgtgct tcttccagcg gtctgaaagc atatagcctg agccatcgaa
     7681 ataataccag ataccgttga ttttctcaaa cttatagaaa ggaggagata gtatggcaaa
     7741 tactccaata aaacctggaa cagataatca gaaacctggt cgctatgtag aggtaggacc
     7801 tcgtggcgga aaagttacta atggtcatac cgcaactatc ggaaaaggtg atcggctccc
     7861 tccgacatct gctaaaggca acggttggaa aaaagtctaa tcttcgtttg cgtacaatcg
     7921 ttcaatggtt gtacgctttt tccatacaca aaagcacatt cccaaaaaat caatttgaat
     7981 ccatgcttcg gcgtaatctt tcccattgct ggcatagtga gttatataat ggtgaatcat
     8041 tttattcctt cctcctattg agttacttga gaagtatcat gaatggcttc atagctaaga
     8101 cgcttaaatt cttctgagtc tatctgaaaa ttgataggct ttttttgtaa acactcaaga
     8161 aaactagtgt ttcttaaaag tttttcaact aactcagggt ctgcctttac aaaggtggac
     8221 tctttttgtc cactatacgg atatcgtttt ggtctcattt tcctactcct caaatctttc
     8281 ctactcaatc ccataatctt caataacctg aagaatgaaa ctgttcgctc gtggaccttt
     8341 agtcgttcca cttagaatgt ttgtcacttc ctgtcgttta aagccgtaag caaccgctag
     8401 agttgctttt ttaatgcctt tctctttcaa gaaagcaata actctttcgc gaccgtttgc
     8461 gatatctggc atattttctc ctttcttttt ctttcttctt tttctgctat aatataagca
     8521 gaaaggagtt aaccttatga cttttaaaga atatttactc aaagcaagca aacgcgacat
     8581 ctacgatgat ggtaaagatt ttgagaaatc aatttcagat tctcactaat cgagcgtgac
     8641 ttttttgata tggcaatctc agaaatttac caccagcgct gactacctta atgacttttt
     8701 caaggtggtc tttttcttgt tctaaatttt taagaatact taccatcagc acgttcctcc
     8761 tctttactta tttgtaaata agaaacaact aaaaatgtaa ctatttttct gcattatact
     8821 tgacaactta caccaaatcg gctaaaatta aagcataata aaaacaccaa taaatctata
     8881 aataccgttc gccaaaacat ttttataatt tatttcttag ttgttttttt agttgtttat
     8941 cacttacaaa aaacatttta cacctttttg gataattagt caaccttttt acaccaaatt
     9001 tgttaaaatt tttttgtgat gtcttagaaa ggttgattta acaatgtttg agacatttga
     9061 aaaaataaaa gaattggcaa aaaagcgtgg aaaagctctt ggacaagtag aagaagactt
     9121 aggttatggt agaaatacac tctataagat aaaaaattct acgccaaacg ctgaacgtat
     9181 cgcagaaatt gctaattatt tcaacgtatc caccgactac ctgctcggac gaacagataa
     9241 ccctgctatc gctgggagtg atgaatttgc ccaagtaaac ggacaaatca tagacttacg
     9301 caaagcagca gccaacacca tgttatttga cgggaaacca ctaaatgaag atgatatcga
     9361 cttcattaca tccgtcctat ccgcccactt caaaagcaaa ggagaacgct aatgactatc
     9421 actatcaact tcacagaaaa aaattcctac attacggact acctaaacaa acacggtatc
     9481 gacacaacga ccatggattt tgacgacttc atggcactca tggaagatat cgaagacgca
     9541 cgagcagccg accaagccta tatggagtat ttagccgacc cagctactta taccatggat
     9601 gaggtcttgg atgaactagg actaactcga gaggatattg cttaatgtat cggctagata
     9661 ttgataaaaa agctctcaag caacttaaaa aactagatac cccaaccaga aaacaaatcc
     9721 tatcctggct tgctaaaaac attgaaaaca cgaccaatcc acgacaacac ggaaaagcac
     9781 taaaagccaa ccttgcaggt tactggcgat acagagtaga gaattaccgc atcatctgtg
     9841 atatccaaga cgataaacta gtcgtcctag ccgtggaaat cgcccaccgc agagatgtct
     9901 ataaataacg aaggagaact atgacactcg ctaaactctg cgaagaatat caagtagaac
     9961 tttgtctctt cgacggttca aactggcaca atagcggttt ctacaatcca gacacaaacg
    10021 tgctcgctat tgaccacaac ttgactcctg aacaacaaat ccaagtcgcc ctacacgaac
    10081 ttggacacaa agaccacaca cgctcagagc accagaacgc ccgtctacgc tgtgaaaacg
    10141 aagctgatat atgatccatc atctcgtaaa agacgctcta gaaaacttag acgaccccac
    10201 agagtttgat tacctcaaat tcatgtctta ctacaatcta aaaaccatga caaatgaaat
    10261 catggttaaa gaagagtatt tagcattagt aaattaaaaa aaggagtatc aatatggaaa
    10321 ttgataaagt aaaagccgat ttaaaacaag tcggcaaacg tgtagcagac ctcagccaaa
    10381 gcattacgaa tgaagaacaa acaaagaatg ccttcattat gccgttcttc caagcgcttg
    10441 gatatgatat cttcaatcca cttgaatttg tcccggaatt tactgccgac gtgggcatta
    10501 aaaaaggcga aaaagtcgac tatgcgatca ttttggatgg cgagcctcaa atcctaattg
    10561 aatgtaaatc aattacagaa aaccttacta agcacgactc tcaactattc agatatttcg
    10621 taactactaa atcaaaattc ggtatcttga caaatggtag agaatataaa ttctttactg
    10681 atttagatga gccaaataaa atggacacaa ctccattctt aacaatcgat gtgactgata
    10741 tcaaagaaaa tcaatttact gaaatcatca aattccacaa agagaatttt gatattgata
    10801 atattgtttc atcagcttct gaattgaaat acctcaacaa tcttaaagcg ttccttactg
    10861 aaaatatcac tacaccgtca gatagcttcc tcagatatct aacttctgaa atctatgaag
    10921 ggcgtgttac tcaaaatatc ttaacgacat tttctcccat tattgtgaaa gggtttaatc
    10981 aattcatcac agagcgagtt aatgaaaaat taagtgctgc acttaataca agcgttgaaa
    11041 ccaaagtaac aaccgatatt ccaaaagttg aagctgaagc tgaagagatt gtcgaagtaa
    11101 ctgacgaaat tatcacaact cctgctgaat tagaagttta tactgttgtt aaaatgcttg
    11161 ctagggatgt agtttctcca gaacgtgtat tttacagaga taaccgtagc tatttcaatg
    11221 ttttggttga cgataacatc aaaaaatggg tattacgtta tcgttcaaat tcaaaaaaaa
    11281 gcactatcga aatccgtgat aaaggcatct tcccggtgtc tacccctctc gaagtcgcaa
    11341 attatgccaa tgaaatttta gaagtaatca agaaattctc ataataaaaa aagccctctt
    11401 tactataccc attttatcaa aaaagtgagg taaaatcaat gtggatggaa gaattgccaa
    11461 acggcaaata taaatttttt gagagataca aagatccata tactgagaaa ttaaaaaaag
    11521 tctcagtaac gatggagaaa aaaactcccc aagcaagaaa tcaagctgcg attttacttc
    11581 aggaaaagat aaaacaaaag ttaggagaaa aacaacattc tgtttctaat ataacttttg
    11641 aaaaactata tgaagaattt gaggagaatt ggaaacacgg cgtaaaaaac tcaaccgtct
    11701 acgcttcaaa gaatgtaaaa aaagagattt taaagcagat agagggcgac tacctagtta
    11761 gaaatttgat agacgtttat tacaaaaagt aatagatcaa ctattacaag atgggagatc
    11821 tcataactat gtttctaaaa tcaaattcaa gctcaatcag ataatgaaat ttgctatcag
    11881 aatgaattat attgatacaa atgaaatgct atttgttgaa acgcctagaa aagtaattac
    11941 atccgacgaa ctcagaaaga aaaatacaaa atacttagac caaaaagagt ttaagttatt
    12001 catccaaaat ttaaaagacg aggccctatg tgattatcga attacaaagt atatccgaat
    12061 agctaaagtt ctttttctta ctggcatgag gtatggagag ctggcagcct taaactacaa
    12121 ggaagatata gatttttcta aaaagactat tcacatcaag catacatacg atttcagaca
    12181 aaaagagaga actacaccaa agacaatcaa gtccgatagg gttataacag cacctcaaaa
    12241 agtgttagat attatcaaag agcaaataat agagaatgcg acaaatggat ttgatacaga
    12301 ttttattttc atcaatactc taggagaacc aataacaaat gccagggtta tttgtgcatt
    12361 gaaaagacat ggtcaaaaaa tcggcataga aaaaaacata actacacata catttagaca
    12421 ttctcacata tccctacttg ctgagctggg cattcccttg actgccatca tggacagagt
    12481 agggcatagt gactcaaaga ccacactaga gatttattct cacgttactc aaaaaatggt
    12541 atcagacata tctagcaagt tagacaagat aaaattttaa attgtgcccc tcgtctgccc
    12601 ctttttctta tacaagacaa acaaaaaccc cttaaagtgt tgattttaaa gggtttttaa
    12661 agtgcacgaa aaaagagcac acaattcaaa tcgcttaggg ctgctggatt cctcccctga
    12721 cccgcttcac gcagaactgt tgctccacta tttattatat cacattccta ttcattttaa
    12781 aagcaaaatt attttttccg tctatttcta aaaaaatcct gcatgatagc tgcgcattca
    12841 tcttccaaaa ttcctatttc aacctccaca cgatgattga gacgctcatc tgtcaagata
    12901 tcgtacaaac tcccagcagc gccaaatttc tggtttttag ccccatagac cacatttgga
    12961 atgcgggcaa gcccaatcgc cccactacac atgacacaag gttcaatggt cacaaaaagt
    13021 gtgcaatcca gcaagcgcca gctctcctca ctcaagttcg catcctctat agccataatt
    13081 tccgcatgca taaccgctcg ctgtaattcc tcacgcgcat tatgcccacg accaatgatt
    13141 tccccatctt tgacaatcac acaaccaatt ggaatttcat cgtgttcaag agcaatctca
    13201 gcctctctca aagcctccct cataaagact tctttttctt caagcgtata atacatctct
    13261 tttctctttt cctacttatc gattttatta ttatatcatg aatcccaaca caaaagaagc
    13321 caccgaatgc ggtgactcta tagggagatt attatgaaaa agaaaagttt aggatatttg
    13381 ttacaacaag ttaggaggtc ttcttgtaac tgtctatagt atacccgacc tatcttaaac
    13441 aaatcttaaa aatctcttag gaccaaacac tttctaaaat atttgtttgt tcacgaccag
    13501 gacctactga gaaagtagaa atacgaacgc caaccaattc actcacacga cgaacatagt
    13561 tacgcgcatt ctcaggaaga tcttccaaat tgcgaactcc ggtaatatct tctgaccaac
    13621 ctggcaactc ttcatagata ggcttgcaac gtttcaattg ctcaagacta gctggatagt
    13681 agtcaatacg ttgaccgtca agatcatagg ccacacagat tttcacagta tccaaaccgc
    13741 tcaaaacatc aatagagttc aaagaaaggt tagtaatacc agaaacacga cggctatgac
    13801 gcatcacaac tgagtcaaac caacctacac gacgtggacg accagttgtt gtaccatact
    13861 catgacccac ttcacggata cgttctccca cttcatcaaa caactcagtt gggaaaggac
    13921 catctcctac acgactcgta taagctttac atacacctac aaccttgtca atcttgcttg
    13981 gaccgacacc agaaccaatt gtcacaccac cagctacagg gtttgatgac gtaacaaatg
    14041 gataagtacc ttggtcgata tctagcataa caccttgtgc accttcaaaa agcacacgtt
    14101 tgccattatc aagcgcatca ttcaagataa cagatgtatc tatcacgtat ttcttgattt
    14161 gttgaccata ttcgtaatat tcttcaaaaa tatcatcgaa aacaatcgct ttactgtcat
    14221 acaatttttc aaaaagacga ttcttttcag caaggttacg ttctaaacgc tcacggaaaa
    14281 tatctttatc taaaagatct gcaatacgaa ttccaacacg agcagccttg tccatataag
    14341 ctggaccaat tcccttaatt gtcgtaccaa tcttattgtc gcccttagct tcttcttgca
    14401 agcgatccaa ctcgatatga taaggcaaaa taacatgcgc acgatcagaa atacgcaagt
    14461 tatcagttgt tacaccttcc tcatgaagat agctcaactc ttttacaaga gatttaggat
    14521 ttacaaccat accattccca atgacagata ttttttcagg gaagaaaatc ccagatggaa
    14581 tcaagtgcaa cttaaatttc ttaccgtcaa tcacaatcgt gtgaccagca ttatcaccac
    14641 cttggtaacg tgcaatcact tctgcattcg ctgaaaggaa gtctgtaatc ttccctttac
    14701 cttcatcacc ccattgggta cctacaacaa caactgaagt cataattttg tctgagccct
    14761 caggctcttc ctttctcaca tacatggcag gactctcacc tgcaattata tcttacaatt
    14821 tattataata aaaaatcgcc tttttatcaa gaagaaacaa tagaaagatt tgctatttcc
    14881 aactattaaa aaatgattta gaaaaattac tagctattta ctattatctt tacataaaag
    14941 agtaaattag ttcggaaatt tactaaaatt acctcaacaa gaaataaaac cccgattcat
    15001 taccaatttt tcaagataca aacgataagc aacacgataa tggtaaacga taaaatccct
    15061 atgacaacca atgccatatc tcactaaata ataaattaaa aatttaaaat gaacatgttc
    15121 ccagtcaaaa ttatcaccaa atgtaggacc atactcttct tcaatactat catagaaatt
    15181 agccatctgc tcataaattt tttgtaacat aatcaaatac tccttttctt ttttataaac
    15241 ttattctaac aaaaaatttt acacattcac tatcaattcc tgaattgtta aaataccagc
    15301 ctcaacaaga taaaaatagg aaaagttgac aaaatagaaa caaatttgct acctaaattt
    15361 caaacaatta aagttctaaa aagaccgcct taaaactttt gggtaaatcc tattagaact
    15421 ataatatttt tatataggta ttatggttct gattttctag tataaaaaaa ttcaaactag
    15481 aattcttctt agaaaaggcc gttttaagtg ctatcattgc tcaaaaatgg agctcgctga
    15541 gacttctatc gtcaagaaga atcatcaaat tccttgtatc atcaaccaaa agattgctca
    15601 aaagttaatt gaaaagattt ctatgaccga cattgatcat cagttgtcta tttcaacttc
    15661 aactgtcatt cgcaagatca ataattttca ctttgagcat gatttttcgc gtcttcctga
    15721 gattatgtcc taggacgttg aaacagtccg gggagtgact gtttcaatcg ggagatggag
    15781 atgagcttta ttgctcagga ctttgataaa ctcaatatca taactgttct tgagagcaga
    15841 acacaagcca tcatccgaaa tcccatgaat acaaggctat caagcgctac tggaagctca
    15901 ttcaacaaga tagtcagaaa ctgagtgata aacgatttta tcgccctact tttcgtatgc
    15961 acttgactaa taaagaaatt ctagataaac ttttgagcta ttccgaagac ttgaaacacc
    16021 actataatct ctatcagctc ttgcttttcc acctctgttt cagactttgt ttaaaacctt
    16081 cctcaaagat aaagagaaaa tcgtcaacgc ccttcaatta ccttattcca acgccaaact
    16141 ggaagccacc aataatctca tcaaacttat caagcacaat gcctttggtt ttaggaactt
    16201 tgaaaacttc aaaaaagaaa ggacgaaatt tgtcctttct agatcttcgc tttcttcaac
    16261 ccactacagt tgacaaagaa ccgaaaaatg tctcgcctct aatatagtca attgaaacaa
    16321 gaacaagaga aaagagcctc gtaaaaggta ttgcaacttg gtaatacctt tttgaggttc
    16381 tttttgatat aagcccatgt tttctcaata ggattgtact caggtgagta gggaggaaga
    16441 ggtaaaagtt tatgcccaaa ctcctcgcat aaaagttcta acttacccat tctatggaat
    16501 cttgcattat ccataataat aaccgatggt gtggttaatg ttggtaagag aaacttctga
    16561 aaccaagctt caaaaaagtc gctcgtcacc atctcttcgt aagtcattgg agcgattaac
    16621 tcaccatttg ttagacctgc aaccaaagaa atcctctgat atcttcttcc agatacttta
    16681 cctctt
//
LOCUS       DAVKLW010000043        16544 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKLW010000043 DAVKLW010000000
VERSION     DAVKLW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.043"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..885)
                     /gene="phtB"
                     /locus_tag="VW324_001476"
     CDS             complement(<1..885)
                     /gene="phtB"
                     /locus_tag="VW324_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9727090.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGL"
     gene            complement(893..1810)
                     /gene="adcAII"
                     /locus_tag="VW324_001477"
     CDS             complement(893..1810)
                     /gene="adcAII"
                     /locus_tag="VW324_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9727091.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDREHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            complement(1953..3344)
                     /locus_tag="VW324_001478"
     CDS             complement(1953..3344)
                     /locus_tag="VW324_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9727092.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            complement(3718..4275)
                     /gene="sdbB"
                     /locus_tag="VW324_001479"
     CDS             complement(3718..4275)
                     /gene="sdbB"
                     /locus_tag="VW324_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9727093.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDDKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(4286..4993)
                     /gene="ccdA2"
                     /locus_tag="VW324_001480"
     CDS             complement(4286..4993)
                     /gene="ccdA2"
                     /locus_tag="VW324_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9727094.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(5224..5640)
                     /locus_tag="VW324_001481"
     CDS             complement(5224..5640)
                     /locus_tag="VW324_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727095.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            complement(5665..5865)
                     /locus_tag="VW324_001482"
                     /pseudo
     CDS             complement(5665..5865)
                     /locus_tag="VW324_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6020..6678)
                     /locus_tag="VW324_001483"
                     /pseudo
     CDS             complement(6020..6678)
                     /locus_tag="VW324_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6892..8655)
                     /locus_tag="VW324_001484"
     CDS             complement(6892..8655)
                     /locus_tag="VW324_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9727096.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(8664..9254)
                     /locus_tag="VW324_001485"
     CDS             complement(8664..9254)
                     /locus_tag="VW324_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9727097.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(9352..9843)
                     /gene="rocS"
                     /locus_tag="VW324_001486"
     CDS             complement(9352..9843)
                     /gene="rocS"
                     /locus_tag="VW324_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9727098.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(9920..10612)
                     /locus_tag="VW324_001487"
     CDS             complement(9920..10612)
                     /locus_tag="VW324_001487"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9727099.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            complement(10629..10943)
                     /locus_tag="VW324_001488"
     CDS             complement(10629..10943)
                     /locus_tag="VW324_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9727100.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(10955..11500)
                     /locus_tag="VW324_001489"
     CDS             complement(10955..11500)
                     /locus_tag="VW324_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9727101.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(11510..12889)
                     /gene="glmU"
                     /locus_tag="VW324_001490"
     CDS             complement(11510..12889)
                     /gene="glmU"
                     /locus_tag="VW324_001490"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9727102.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            13033..13833
                     /locus_tag="VW324_001491"
     CDS             13033..13833
                     /locus_tag="VW324_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9727103.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            13928..14770
                     /locus_tag="VW324_001492"
     CDS             13928..14770
                     /locus_tag="VW324_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9727104.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGALAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLTWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            14770..15252
                     /locus_tag="VW324_001493"
     CDS             14770..15252
                     /locus_tag="VW324_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9727105.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            15252..15872
                     /locus_tag="VW324_001494"
     CDS             15252..15872
                     /locus_tag="VW324_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9727106.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(16126..16221)
                     /locus_tag="VW324_001495"
                     /pseudo
     CDS             complement(16126..16221)
                     /locus_tag="VW324_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5020 a         3489 c         2901 g         5134 t
ORIGIN      
        1 aaggccatca gattccacat ggcgttctga taagggttta gcatacaatt cacgtaaaag
       61 gcttgaaatg ttttcccctt gattttgatg ataagttgga gtgacagtca gattgtggtt
      121 ctctgacaat cttggttgag ctggatttgc attataacta gaacttgaag aaggacgaga
      181 tccctgcttc ccattccaat aggcttctgc agcagctaac tcgctagctg ataactcatt
      241 cttaggaatg taatggtaat ggtcgccgtg aggaacgata taagcatcac ccgtgtcctc
      301 aatgatatca gatgcattga agatataccc atcatccgtt gtataacgtc cttgggctct
      361 ggctgcagca acagcattat ctgctcttga gttatgatta tgactgcgtt cctgcttctg
      421 acgtttaatc tcttcttttg tccgaatatt atccgcatga gctgcatcct taaggtaaac
      481 atagtattta ccgtttacct taatgacata accacccttg atttcattga caatgtctga
      541 atccttcaac tgataattcg gatctttcat gaggagctct tcactgatga tggcatcata
      601 aggaaccttg ccattatagt aatgataatg gtctccatga gaggtcacat aaccttgatc
      661 cgtaatcttg ataacaattt gttcggcgtt gatcccctcc ctcttactga cttcatctgg
      721 tgtcaagttt tctgcctttt gaccagcctg atcaccatct atataagcaa ctcgattaga
      781 ctctttctta tcctgaccag cttggtaacg tccaagctca taggaacaaa cacttagggc
      841 aaggactgcc actgaacctg ctagatattt tttattaatt ttcattcttt cctcacttta
      901 attcttctgc tagaatactc atattttctt caagattttc taaataggtc ttgtcatttt
      961 gtgggtctga ctctaaagga ttcagagttt taagacccac acctgttgat ttgacaagag
     1021 tttcagctac ttttgaagaa gcgttacttt ctgtaaaaat cgttttaacc ttataggtct
     1081 taacaaattc ctgaatttct gttagttgtc gtggacttgg ttcttgttca ggagagatac
     1141 ctgcaatacc aagttgatta agcccaaatc tcttcgctag ataagaaaag gctgtatgtt
     1201 gtgttacaaa tgttttctga gtcgcttttt caaattttgg ttggaatttc ttagtcaatt
     1261 cctgagcttt tttgataaag gcttgcgcat ttttttgata agtctcttta tgctccctat
     1321 ccacctctga aagtttatca gcgataattt gggcttcttc tccagctttt tcaggatcta
     1381 gccatgtgtg agggtcatag agcgtttttt catcaactcc atcccctgct tccacatcct
     1441 ctagtccagg gacacgttcc aaggtcattc cctcagaagc ctctaagacc ttcactttgg
     1501 atttttttag atttggatcc agacttcctg cccaagattc gagtgtatga gaatggtaaa
     1561 caaagacatc tgcatcatag atggctgcga tatcatttgc cgaaggttca aaggagtgaa
     1621 taccactact tgactgaatc atccgaacat cattcaagtc accagatact tccttaacca
     1681 tagcgtagat aggataaaaa ctggtcacaa ttttcatccc ctttcctgtc tgactttcct
     1741 tttgaccaca agcccccaag cacaaaagaa agacacttaa caggactaaa aataaatttt
     1801 gtttcttcat gaacaactcc ttaaccattt aattaactag taaatattct actcctgaaa
     1861 atttaatctg tcaagatatt tttagaaaaa atttgaaatt tctttcacat ataaaaatct
     1921 actgagtgtc aacaactcaa tagactttca ctttattctt ctatggtaga atgcttatag
     1981 ccataagtga agtaaataat acttcctact aacaacgcaa ctaggaaagc aatccaggta
     2041 ttcatattat actgtaacat gaaggataaa caaatgatga tcgataaaat tggtaacaga
     2101 ggtaccaatg gtgttttaaa ttccccagct ttgggcattc ctttttcttt ccgtaagcga
     2161 atcagaccat aagccaacat gatcaagtag gctaaggtac aaatatttaa aaaggctgca
     2221 atactggcta gtgggaacat tcctgctgct actgctgaag ctagacctgt caagatagta
     2281 gcattctttg gtatcttgct agtcttcgtc agttccttaa aggcagcagg cactaagccg
     2341 tcacgtgcta aactgtaaat catacgcgat agggcatagg tcatcgaaat acaaactgta
     2401 atcaaggtca agatagccac taatgacaca tagttggctg cccaactaat cccaacacta
     2461 cgaagggcaa aggcaacggc atcatcgaca tttaaatgac tatagtgaac cacaccagtc
     2521 aaaacaaggg tcaccaaggc atagagaatg gttacgatag aaagcgataa gacaatccct
     2581 ctaggaatat ttttttgagg agtcttgact tcatctacag ccatagagat ggattcaaat
     2641 cccaaaaaac cgaagaacat caaggatgca ccagccataa taccagtact agctccatag
     2701 atttgtccaa aaccgtaagg agcaaaattg ctccaattat caaacttaat attccaaatt
     2761 cctactaaaa caaagagagc taaagcggaa aatttcaaaa taactagaat cgaattaaag
     2821 cgtaaggctg ccttggcatt tagtaaaaca agcgaagtca ccaagaccaa gacaagaata
     2881 ggcaataaat caacaaatgt ccctgcttga ggattaaagg taccatttaa agcctgagga
     2941 aaggctatcc cgtattgaga gagcaagcct ttaaaataag ctgcccaacc cgaagctacg
     3001 cctgatatgg ctgtcatgaa ctccatcatg gttaaccaac cagccaacca ggcagggaat
     3061 tctcctaaga tagcatagag gtaactatag gcacctcctg tagcgggtac tcgcgaggca
     3121 aattctgcaa aaaagagggc tgataatccc acacacaagg cagaaataac gattgaaatc
     3181 actagggctg ggccagcaag tgttgcagct gcagtacctg tgattgtaaa gacgcctgtc
     3241 cctaccatgg ctccgatacc cagcaaaatc aaatcccata acttcaaatg cctatgcatc
     3301 tctgttttat ctaaactaac attctttgtt ctaaatatat tcatctttga ctccaaaata
     3361 aactgataat tctattttac cataaatata ggagatatgt gaatatttat aaaatgtttt
     3421 tctaaaaaaa tctgactacc gtaacttctc aaagtaactt ccatctctct cccaaaactg
     3481 gaagttactc taagacgact ctgttttagg gctatttgtc gtgcactttt tctgagtctt
     3541 ttcgcttttg aacccttata tatcaagaaa aagaaaacca gtggaagtta gtctaagacg
     3601 gatttccact gatgtcacgc gtttttctca tactaaaagc taggttgtgg gaaactggaa
     3661 gttagtttta gaagttacca aaatctgact acatcttgta atcagatttt atcgattcta
     3721 gttcatttct ttaaatgctg cttctgcatc cgcattactg atagcaccaa attgaatctt
     3781 tccaatcttt ccttggctat caattaaata ttctgtagga atgcttcgaa tttgataagc
     3841 ttggaaggtg gttgctttgg tatcataaag aactgggata tccttatatc cttgttcctg
     3901 gaaccattgt gggaattgct caacagtttt ttcaccttga attcctggtg caatgacagt
     3961 aagaatttcg aaatcacgat ctggtttcgc cgctagttcc atcaactctg gcatactttt
     4021 cttgcatgga ccacaccatg aagcccaaaa cttcaagtaa acctttttac ccttaaaatc
     4081 agataactta acttctttgt catccatgga ttgcaatgta aagtctggag catcttttcc
     4141 aacagaaatt tgttgtacag tcgtttgttg ttttggctgt tgtgctgctt gagtcttttt
     4201 agtttcttcc tcaccacagg ccatcaatac aactaatgac aagagactta agccagcaaa
     4261 cattactttt ttcatttgtc ctcctttatt caaaaattcc agctagaaca tttacttgtc
     4321 ctaatagtaa caaaattccc attaaaacga tgaggaaacc accaattttc tttagtagca
     4381 tcatatgacg cttgatttta ctaaaatatg gcatgactag acctgaagct agtgccaaga
     4441 ccaagaaagg aagggccatg cccagagtgt aaatgagagt ataaatcgct ccttgccaag
     4501 caccattgcc tccagaagcc gcaagtgcta aaacagaact taaaactgga ccaatacaag
     4561 gtgtccaacc aaagctaaag gtaataccaa gtaaaaaagc tgaccaataa cgattagaat
     4621 ctgatttttt aaaggtaaaa cttttttgaa cttctaattt cttcaaatga aaaatttcca
     4681 tctggtgaag acccaaaatg ataataatag ctcccatgac atatcgaaac caatttgcat
     4741 agagaatatg accaaagtaa ccagcaccaa agcctagaat aaagaaaatg agagagatac
     4801 cagcgataaa gcaaagtgtt cgaatcaagc ctgaccagag aacctttctc ccaaacaaag
     4861 aaaagctttt tgcactttct tgatcatcca ataaaatccc agcatagact ggcagaagag
     4921 gaaaaataca aggagaaaaa aaggataaaa cacctgctag aaaaacagag attaaaaata
     4981 ctatcgtttc caataaagaa ccaactttct taataattct aatcctattt tactatattc
     5041 aattttattt gtaagctttc tgctacgcaa aatcgtatcg ggcactattg gaccaatctt
     5101 ttcttttgct agtcaaggcg gatcttatcc cccaaaatag ccaaaaagca acgacaagga
     5161 ttactcgtca ctgcttttgt gaacgaaaat gtcttttagg tctgacattt cataaatcat
     5221 gttttacttg agtttgtcaa ggattgcttt aagctcctct actagtttag tttctgtctc
     5281 tgctgagcca ttttcttctt tcacgaaatc aagggtttct tggagaaggt tttgggcttt
     5341 ggcaaggact tttttatccg ctttttctgc atctagctgt cctagaacct tgatcaattc
     5401 cgtgcttaat tgctggattt ctgactcttt cttacggcga atcagccaga aggcaatcac
     5461 gcctaggagg gcaagtagac tgaccacaat cactcctgcc ggaactgagt ttgtttcagt
     5521 catcttatct gaatccttac tatcttccgt tccttgtttt gcatccttct tgtcctgtgc
     5581 aggcttgctg tcgctagcat ttgctttcac atctttgaga gagtccaagg cagccttcac
     5641 agactctact gcagtatgca gaccttactc tgtcaaggca ctatcttccg gagctttttg
     5701 agcatctagg aggacagcct tggttgcatc gattttcgga tcagatactg ttgccaaagc
     5761 tttcaagcgt tggtctaact cttgactcaa ggcacgaagt tcagacttgt caacttactc
     5821 ttgagcttgt gtgctcgttg agctagccga agcgcttact accactctag gatcttgagt
     5881 cggagctgag cttggagctg gggcaggact tgcaggttga ctaggagcag ttatggtata
     5941 ttgaaactag aatagtacat atggacttct aaaacattgt tagaattcga ttttactgtc
     6001 ctgatcgatt tgtcctattc ttatttcgtt ttactataat aaccgatggt gtggttaatg
     6061 ttggtaagag aaacttctga aaccaagctt caaaaaagtc gctcgtcatc gtctcttcgt
     6121 aagccattgg agcgattaac tcaccattca tttgttagac ctgcaaccaa agaaatcctc
     6181 tgatatcttc ttccagatac tttgcctctt attaactgac tttttaatga gcgaccatat
     6241 tctcgataaa aataagtatc gaatcctgtt tcatcaatct aaacaggtgc taggtgcttt
     6301 aatctattaa aattcttaag aaataaggct actttttctg ggtcttgttc atagtaggtg
     6361 tggttctttt tttcgagtgt agcccatagc tttgagcgca tagtggatgg tagttggatg
     6421 acagccaaat tcagaagcta tttcagtcaa ataagcatct ggattgtcag taagatagtt
     6481 tttaagtcta tctctatcaa cttttcttgg ttttgttcct tttacttggt ggtttagctc
     6541 tcctgttttc tcttttagct ttaaccagcc ataaatggta ttacgtgaga tttggaaaac
     6601 gtgtgatgct tctgttatac tacctgttcg ctcacaataa gagagaactt ttttacgaaa
     6661 atctattgaa tatgccataa gaagattata ccacattgtg tactattttt ggttcatttt
     6721 actatatttt agaagttata gtgtagcatt ccaacttcaa agcactataa agtaaattga
     6781 aacaagaaca atacaaacaa ttctcgtaaa cggattgcaa ccacaaaaaa gcaagcattc
     6841 acaagaatac ttacctatca tgggaggaac aaccgttcct cttttttatt actaaaattc
     6901 aaagaattcc aatgcttttt tcaagagcaa atccgtatat tctggatctt cttgggctac
     6961 ttctatttcc cgctgaactt tttccaaatc atctgtaatc actccatcta ctcctaagtg
     7021 aagagatttg ctgatagctt ctgaatcatt gacagtccag acataaagtt tctgatccgt
     7081 tgtccatagt ttgcttacaa aatattcatc caaggttgag tactccatag tatatcctgt
     7141 cgctcttgtt ttaggaaaga cagaattgta gggcatgatg aaataaactg gcagttcggc
     7201 atcatactgt cttacttttt cgacaacatg gtagtctaaa gactggattt gatgtccata
     7261 aatcttgagc tttgcagcat aacgggctaa aaagcggttc atcatgtctg gactatcttt
     7321 tttactggtt ttaatttcaa ttagtaattt ttgaccaagt tcgttggctc gactgagata
     7381 atcttcaaag cttgaaattt tagtctggta gccattttca aaaatatcaa tccctttaag
     7441 ctcctccaag tttaagtctt gaggactttt attgatacct gctagatttt tcaagttagc
     7501 atcatgcatc atgacaaact gcccatcttt tgtttcctgc acgtccgtct ccaccaagtc
     7561 tggtttgagt tgtgctgtag tttccaagga ctctactgta ttttgaatcc catttgcatt
     7621 ggaaacccct cggtgagaaa taagttgagg tagatgaacc atgggagcct ccagataaat
     7681 ataaccttct aaggcaaaga aaaaactggc acaagtcatg acaccccatc gcacgatgtg
     7741 atctttttct ctcctaggaa gcatatccag ctcctttcct gtcaaaaatg aaacaaattt
     7801 aaccaaaaaa taagtcagag ccatataata gagattttta atcacgacaa aattcaaaat
     7861 accaagaatc agagactctc tctgagtgat atcatctacc aaagtttgag ccaataataa
     7921 aggaatcaaa ggaagataaa ataataaatg tgctttgagc aagatgtaaa ataaattcca
     7981 agcataaaaa gtaactctct tcttggtttt ctccaagcta aacatcactg cttctcgaac
     8041 agtcagctga tcatatacaa tcttcggaag ggcaaacatc aatctgacag agacatagag
     8101 aaagataaga gatagaagta ggatgctcag ccaccacatc caatatttat cttctaaata
     8161 agcttggata aactctggaa tgacgatttt attaagataa taaatcttca gcattttccg
     8221 tataaaagga aacagcatag ctatatagaa aaagataaac aaggctttag cgccagttag
     8281 ctttttcata aattcaaaac tttcatggaa aaccttgcgg atatactcaa ttagccttcg
     8341 cttttcatta tagaggagat gacgagcacc aataaagagg agtcctattt gaaaataagc
     8401 aaccagaaag ttaattacaa tcaaggctaa aaaagctaga ctaatcaatg gagaatgagt
     8461 aaggatggct aagacattgt tataggaaat aaaaagataa cctgtctgat ctaataagaa
     8521 gctagccaac catgaattga atggtaccca caaatactcc actatcataa aaatcaagaa
     8581 aaatagaaag aggattttat caagattgag gtaaatctgt ttaagaccca attttttagg
     8641 tttttcaggt ttcataggca ctcctagtca aataattgag acaagtccaa gccaccaaaa
     8701 ggattgtttg ataagctact ttctgtctct aacaattccc tagcttgatc cgactctaag
     8761 aaggattcgt aaacacgcgc cgtcatccga gcatcctcta aactattatg agactgacct
     8821 tgaaatccaa gaaatgaggc aacagtttgc aatttgagat tggcaatacc atgtaaatct
     8881 gaactccgac gttcaaaagc ttcatcatac aaatccacct tgtactgttg gctatagtct
     8941 aaaccatgct ctgctaaaat aggtaaatca cttttagcag cattgtagcc tatcatcagc
     9001 aaagaaccca caaactcttg gaattcttct aagactttct caactggagg agcatctttt
     9061 aaggtctcag ctgtaatccc ggtcaaacca ttgataaagc tttttagagg gacgctggta
     9121 tgaacatagg aatcataggc atcgatttcc cgaccatctc taaaacgcac tgccgatact
     9181 tgaatcaagt gtgtttgtcc ttcgtgctga ttaaattcta aatcaaaagc aatgtaatct
     9241 tctaatcgtt ccattctgta cctctcctct actgtctctg ttctaatcaa ctatactatt
     9301 cacaaacaaa agaagccatc aggttagcat ttaacctaaa cagctccttg attatcctcc
     9361 aaataaacga gcaaaaaagc cttttttagt ggattggact tcttcttttg cttggtccag
     9421 ctctagctta agattttctt gatccttcat ggcttgaagg gtcaattgtt gttgctgatc
     9481 taattgtttg tctttctcag caatctgacg gtctttgata cgcatctgct cgtctttttc
     9541 tgataactga cgatccttgg cttttaattg ttcataaaga cgaagaattt ctgcattctt
     9601 ctcatccact agaatctcca tcagttcacg ttgtttgaca tcttcactga caggttcatc
     9661 ttcaaaaatc gtttttttat agatttcttc tagcttaatc aagccacttc tggtaacaac
     9721 tgttacccct ttgtcatttt tatctgtgtc ttcttctggt aattctttga cacggttatt
     9781 gattgcttgg cgagataatc ctaagacctc tgcaatctca ctgacggtca tttcaatact
     9841 cataatatcc tctgaaacgt tttctagctt ttctttactt aaatcttatc ataagctaga
     9901 aaaactgtca aatttccgct taatctaaag ccttcaaaaa ggccaacaag acttgggcag
     9961 agcgacgtcc agcttcgata ataaactcat caaaaaagat gtttgcttca tggttggcat
    10021 tgtcactcat agctcggatg actaagactg ggagattgag ggtatgcgct gcttgagcaa
    10081 tagctgcccc ctccatctcc acggctaaaa cttctgggaa atgggactta atcgcttcta
    10141 tcttgtcatt tcctgcaaca aaactatctc ctgtagcaat caaaccaaga tgccagtttt
    10201 ggtccaattg agataaactc tcttggattt gagcaacaaa ggttttgtct gattcgaaat
    10261 aaagcggttg ttgcgccatt tgtccataag catagccaaa agctgtgaca tccacgtcat
    10321 gataggctaa tttgtcagca atcacgacat ccccaacagc gataccttct gctactgccc
    10381 cagctgaacc cgtattaata agggcatcca cctggaaatg atcagccaaa atcgccacac
    10441 tcatagcaga catgacctta ccaattccac tttctacaag aacgacttca tgagaagcaa
    10501 tggttcctgt atgataggta ttccccaaaa caacttgctc ctggacatta tataaatgct
    10561 ggaccagata agccagttct tctggcatag cagcaataat tcctattttc atttcaattc
    10621 cttttctatt acaaaagttt cattgctaaa acaagcaaaa tcaagagaaa gatgaccgcg
    10681 aataagattt tattcaactt agaattgaag acatttctct tggtattttc aatgcgacgg
    10741 cttttatgaa tagatggttc gacctgaatc ttcaaggttt cctggctaaa accatgattc
    10801 ttaggattgg cataaccaaa acgggaagaa gaggttggta aaatcttagt ctcctcatta
    10861 tcatctagca aaggaggacc tgaaattttt tcgcctctat tagctctttc aatcatttca
    10921 tccgttaata aagatttacc catactgacc tcctctattt tttctgcaac tcccaatact
    10981 gaacagccat aattgtcttg gcatcacaaa tatgacctga ttggattaat tctttcgctt
    11041 cttctaagct cacttcaagg acttccaagg tttcatcctc atcctgcgga cgcggatttt
    11101 ccacttttgt caaatcgctt gctaaatata gttttaactt ctcattacaa aagccaatag
    11161 ctgaataaaa atcgtacaag agttctaatt tccctgtata ggctgtttct tcctctaatt
    11221 cacgaagggc agctgccaca ggggctgtgt tttctcctac ttccaatttt ccggctggaa
    11281 tttcgtaaga gacagcctcg atagctttgc ggtactgctt gaccaagatc agtttttgtt
    11341 catccgttac tgctaaaaca cagacagccc cattgtggaa aatcaaatcc cgttgggcag
    11401 ttcccttgcc ttctggtaat tcaacctgat cttggaccag tttaaatatt ggtccttgat
    11461 agatttcttt tcggctaagc gttttttctt caaactccat gataggctcc tactggttct
    11521 taggatgatg aggaagacgt gttgcatatt cgtctttatt gatctgacga ccgcgaccaa
    11581 tagcaatagc atctgctggc acgtctttag taatagttga accagcacca acgagggaat
    11641 tgtcaccaag ttctactggt gcaataatgg ttgaatttga accaacaaag acattgtctc
    11701 caatgactgt cttgtatttg tttttgccgt cataattgac tgtaatagtt ccagcaccga
    11761 aattaacgtt gcttcccact tcacagtttc cgatataagt caaatgacca gccttggtat
    11821 tctcaccgat tgaagatcct ttcacctcaa caaagttacc aatatgaact tgggcaccca
    11881 gacttgaatt tggacgaatg tgagcataag gaccgactgt cacaccgtct gcaacactac
    11941 tttcctcaat catagaattg gtaatgaccg ctcctgctcc gatagtgctg tccactacat
    12001 aagtaccgtt tgtcaaaaca gtctcagcac caattttcgt ttgccctttc aaggtaacat
    12061 tggcttcgat ttgaacttcc ggagcaatct caacatcaat atcgatataa gttgcttctg
    12121 gattgacaaa gctaacaccg ttgaccatat gtttatgatt gatgcgacga cgcataactg
    12181 actcagctgt cgcaagcgcc acacggtcat ttaccccaag actttcatca aaatctttca
    12241 aagtataagc gccaactttt tcaccagttt cacggaaaat accaatgacg tctgtaatat
    12301 agtattcgcc ttgagcgtta ttggtattga tatttttcaa agcctcaaac aaacgctcgt
    12361 tgtcaaagac gtatgttcca gtgttgattt ccttgatttg cttttcaaaa tctgtagcat
    12421 ccttctgctc aacaatacga agaacctcag cattgtcatt acgaacaatt cgtccatagc
    12481 caaaaggatt atccgtttca gcagtcaaga tagtggccac atttttatga ttgatatgga
    12541 aatcaatcaa gtttttcaag ctttcaccag tgattaaagg agtatctcct gcaatgacca
    12601 aggtgtgtcc tgacaaacct tctaagatag gttctgtcat cataactgca tgaccagttc
    12661 ccaactgttc agattgagtc acaaattctg tctgtccagc caagacctcc tcaaccaatt
    12721 ctgccttgtg tcctacaact gttactgtct tttcaggttg gatagctccc acactacgga
    12781 aaacatgttc caacatagaa atacccgcaa ccttgtgcaa aacttttggc aaatcagatt
    12841 tcatgcgagt ccctttaccc gctgctaaaa taatggcaaa atttgacata atcttctctt
    12901 ttctgtagaa attccctttt attataccat atttcaaatc attccgcact cttgaatgaa
    12961 aaaatgctct aaagtctctt ccttaaccca tttttttgat tttttttgat ttttagggta
    13021 gaattataag atatgagaaa acaaattcat ccacttattt tatttagttt tttggggatt
    13081 atgatgttca ttttaatcat caatcgccca cttaatgaca accaatcgtt taaaacaaaa
    13141 tccaatatag cacagattga agcacaggct ttgaaacact tagacaaacc gattattgac
    13201 ctctctggct ggcagcgtcc tgaagaaatc aactacgatg ccctctcaca aaatatttca
    13261 ggtgctattg ttcgtgttca cagtggtgct caaacgacaa aagaaaatga tgcttcattt
    13321 atcaatggaa tagacaaggc ctataagagt catatcactg aacttcaaaa gcggaatgtc
    13381 ccagttgctg tctacgctta tgtagctgga aaaagtgtcc aagaaatgga aaaggctgcc
    13441 gaagttttct acaatgctgc ctctccttac agccctagtt actattggct tgacgtggaa
    13501 gacaaaacca tgtctaatat gaacgaaggt gtcgaaaact ttcgagcaaa actagcatca
    13561 ctaggtgcta aaaacatcgg catctacgtt ggtgtctact tcatggaaga acacagtatt
    13621 gatacaggca agtttacctc tgtttggatt ccgtcctacg gctcagactc aggatttttg
    13681 gagagcagtc ctaaaactga tttagattat gatattcacc aatacacatc taaaggaaaa
    13741 attgccggct ttgaccacga tttagatatc aacgttatct ctcccttaaa aaataaagaa
    13801 gaaacctttc gaaaactttt tttaaaacct taaaagcatt tgtttatcat ttgctttttc
    13861 tttttgtgtc tgattgtgat acaatatagt aaggattttt tgaaatagaa atagttggag
    13921 gaaatatatg ctctcatggt tagcacgcgt tattaaaggg attgtaattg ctcttggatt
    13981 tatcctaccg ggaatttccg gaggggctct agcagcaatc ttaggaatct atgaacgaat
    14041 gattggcttt ctggcccatc cctttaaaga ctttaaagaa aatgttttgt actttattcc
    14101 agttgccatc ggtatgcttc taggaatcgg cttattttcc tacccgattg aatacctgct
    14161 tgaaaattat caggtctttg tattatggag ctttgcggga gctattatcg gtacagttcc
    14221 tagcctcctc aaagaatcaa ctcgagaatc tgaccgagac aagattgatt taacttggtt
    14281 atggacaacc tttatcattt ctggattagg actctatgcc ttaaattttg tcgttggaac
    14341 cttaagcgcc agctttctta acttcgtcct agcaggcgca ctattggccc ttggcgtctt
    14401 ggttcctggc ctcagcccat caaatttact tttgattttg ggactctatg ctcctatgtt
    14461 gactggtttt aaaacttttg atctcttggg aaccttcttt ccgattggaa ttggtgcagg
    14521 tgcaactctc atcgtttttt caaaattgat ggattatgcc ttaaacaact accactcacg
    14581 cgtctatcat ttcatcatcg gtatcgtcct atcaagtacc cttttgatct taattccaaa
    14641 tgcaggaaac gctgaaagta tccaatacac aggactttca cttgtcggtt atgtcatcat
    14701 cgccttcttc tttgcgctgg gaatctggct tggtatttgg atgagtcaat tggaggataa
    14761 atataaataa tggcaaaaaa agttaaaatc aaaaaaacat tggtggaaca aatcctatct
    14821 aaagcagcta tccctcatca gggaattcaa atcaatgccc tagaaggaga gcttcctcaa
    14881 ggttatgaac gagatcagat tttcaaaacc ttggcgcttt tgggagacaa gaccggaccg
    14941 attatcggaa ttgtccctat cactcagcac ttgtctgaaa agaaactagc taaaatttct
    15001 ggcaataaaa aagtgagcat gattccacaa aaggacttag aaaaaacaac tggttacatt
    15061 catggagcca ataatcctgt cggaattcgt cagaaacaca attatcccat ttttatcgat
    15121 aaaattgctc tagatttgga tcaaatgatt gtctctgcag gcgaagtcgg acacagcatt
    15181 atcgtcgcgc cacaagactt ggctagcttt gtaaaggcag actttgtaga tattttggag
    15241 gacatcaagt aatgaaactc tactttgtcc gccacggtcg taccctctgg aaccaagaag
    15301 gtcgttttca aggtgctagt ggtgattctc ctcttcttcc tgaatccatt gaaaccctaa
    15361 aacgacttgg tcagtatctc aaggaaattc cttttgatca gatttattca agtgatttac
    15421 ctcgagcggt caaatctgct gagattatcc aaagtcaact ctataccccc tgttctttag
    15481 aaatcgttcc taatctccgt gaatggcaac ttgggaagtt agaaggtttg aaaattgcaa
    15541 ccttggaagc tatttacccg caacaaatcc aggcttttcg ttcaaatctt gctcaatttg
    15601 acactcgaat gttcggagcc gaatccctct atagtacaac tcaacggacc atccaattta
    15661 tcaaatcatt aaaagatagt ccagctgaac gtattctaat tgtcggacac ggcgccaatc
    15721 ttactgccag tcttcgtact ctcctaggct ataaagaacc acttcttcgt aaagatggcg
    15781 gtctagcaaa tgccagcctg accattatag aaacccatga ttttgaaaca ttcactctca
    15841 atacttggaa tgatacttct tatcaatcat aattatagcc tagccttctg aactagaacc
    15901 ataggagata tagtcagttg aaacaaaaat aagataaaag agcctcgtaa aaaggtattg
    15961 caacttgcca ataccttttt tgatatgaac acgtgtcttc tcaacaggat tgtagagcaa
    16021 aatgaaataa gaactgaata atatgatttg agaatttaaa tcaatttata agaatgtttt
    16081 agaagcagtt atgtactatt ttagattcac ttttctatac taagatgagt agggaggaaa
    16141 aggtaaaagt ttatgcccaa actcttcaca taagaactct agcttaccca ttctatggaa
    16201 tcttgcatta tccattaaaa cttccagact catacccttc taaaatctat tcaaaccacg
    16261 tcagcttcac attatatatg ttggttacct cacaagtttt atctgcaacc tcaaagctgt
    16321 tctttgagca gcctacagct agcttcctag tttaatcttt gcttttcact gagtatcaag
    16381 ttaaaaaatg aggctggtca aaaagtcttt aaaataaaaa aaacgcatat tatcaggtgt
    16441 taaatgaact gcaccccaaa agttagacag aaaaaatcta acttttggag tgtttttatt
    16501 atgaaattaa cttatgatga taaagttcag atctatgaac ttag
//
LOCUS       DAVKLW010000044        16490 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKLW010000044 DAVKLW010000000
VERSION     DAVKLW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.044"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(125..841)
                     /locus_tag="VW324_001496"
     CDS             complement(125..841)
                     /locus_tag="VW324_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9727107.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1189..2976
                     /locus_tag="VW324_001497"
     CDS             1189..2976
                     /locus_tag="VW324_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9727108.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2973..3449
                     /locus_tag="VW324_001498"
     CDS             2973..3449
                     /locus_tag="VW324_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9727109.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3477..4382
                     /locus_tag="VW324_001499"
     CDS             3477..4382
                     /locus_tag="VW324_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9727110.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4369..5184
                     /locus_tag="VW324_001500"
     CDS             4369..5184
                     /locus_tag="VW324_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9727111.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            5191..5595
                     /locus_tag="VW324_001501"
     CDS             5191..5595
                     /locus_tag="VW324_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9727112.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            5894..7060
                     /locus_tag="VW324_001502"
     CDS             5894..7060
                     /locus_tag="VW324_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9727113.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            7176..8213
                     /locus_tag="VW324_001503"
     CDS             7176..8213
                     /locus_tag="VW324_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9727114.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            8421..9275
                     /locus_tag="VW324_001504"
     CDS             8421..9275
                     /locus_tag="VW324_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9727115.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            9412..9669
                     /locus_tag="VW324_001505"
     CDS             9412..9669
                     /locus_tag="VW324_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9727116.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(9711..10073)
                     /locus_tag="VW324_001506"
     CDS             complement(9711..10073)
                     /locus_tag="VW324_001506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727117.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMR
                     FFNFRDFLQLLLTHIKLF"
     gene            complement(10127..10423)
                     /locus_tag="VW324_001507"
     CDS             complement(10127..10423)
                     /locus_tag="VW324_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9727118.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(10413..10574)
                     /locus_tag="VW324_001508"
     CDS             complement(10413..10574)
                     /locus_tag="VW324_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9727119.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            10644..11408
                     /locus_tag="VW324_001509"
     CDS             10644..11408
                     /locus_tag="VW324_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9727120.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(11648..11800)
                     /locus_tag="VW324_001510"
     CDS             complement(11648..11800)
                     /locus_tag="VW324_001510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727121.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            12018..13397
                     /locus_tag="VW324_001511"
     CDS             12018..13397
                     /locus_tag="VW324_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9727122.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            13411..14076
                     /locus_tag="VW324_001512"
     CDS             13411..14076
                     /locus_tag="VW324_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9727123.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            14190..14285
                     /locus_tag="VW324_001513"
                     /pseudo
     CDS             14190..14285
                     /locus_tag="VW324_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14431..14969
                     /locus_tag="VW324_001514"
                     /pseudo
     CDS             14431..14969
                     /locus_tag="VW324_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            15036..15446
                     /locus_tag="VW324_001515"
     CDS             15036..15446
                     /locus_tag="VW324_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9727124.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            15562..>16490
                     /gene="pavB"
                     /locus_tag="VW324_001516"
     CDS             15562..>16490
                     /gene="pavB"
                     /locus_tag="VW324_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9727125.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASLAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNV"
BASE COUNT         4629 a         3086 c         3719 g         5056 t
ORIGIN      
        1 ttggttctat ttctataaaa tattccacaa attatagaat cttccaaaaa taggtaagcg
       61 ctaccttttt ggtgtggtat aataagcata gaaaaagccc aagcgattag ctcaggtttt
      121 cttcttagtg atcacggtca catgagataa atttaatctt gtagtaatca gatcgtttgt
      181 aagtttcact gtattctaaa acttggccag ttgattcgag tttggtgatt ttagtttgta
      241 ggacagtagg gaattgttca tcgactccga ggactgaagc cgcatgttct ggagttggaa
      301 agactatttc gttgatttct tcaaagtgtt catcattcat gtgaatgtgg tagtctaact
      361 tgaaacgatt atagatagaa ctatagtatt caaggtttgg ataatttgcg ttgatatatt
      421 gttctgggat gtaggatgta tggtagatat aaacgacacc gtttgattcg cggatacgtt
      481 caatcttgta gtagaattga tcgccgcgta gacccaattt ttccaagtaa acaagcttgt
      541 ttccgcgttc aattgaaaga acagttacct tatcatcttt agcattgaag agctcaatat
      601 ctgaaaactc tacaagcttg tgtttgcgtg cacgtgaaac gaaggttcct tttccttgtt
      661 ggcggacaat atagccatct ttggcaaggt cgtttaaggc gcgaacaact gtgatagagc
      721 tgacatcgta cattgaaatg agttctgctt cagtgtaaaa tttatctcca ctgctaaact
      781 gcccagagat gattttattt tttaattcgt cttttatgta ttgatatttg ggaattgcca
      841 tatttttacc tctattttcc ttctccatag aaaattttac cataaaagcg aaaaaaatag
      901 tagtcttttg aataaaaaat tagaataata gtttgaaaaa ttaagcgata tagggattgc
      961 tcatatttta tcaatgcatt ctatacaaaa ttattttagg tcctatattc cgaatttaaa
     1021 gagcgtttga aatgggtcta aaaatgtttg aaactaattg aatgtgtgta taatagtaaa
     1081 aatgcagtaa atatcccgac aatttctatt gacaattcaa acagattggt ttataattaa
     1141 tataacaaca aatgaaagcg caaactttcg cggtcggaag gtagttttat gacacgattt
     1201 gagatacgag atgatttcta tctcgatgga aaatcattta agattttatc tggtgccatt
     1261 cattatttta ggattcctcc agaggattgg tatcattcgc tctataactt gaaggctctt
     1321 ggttttaata cggtagagac ttatgttgct tggaatttac acgagcctcg tgaaggtgag
     1381 tttcattttg aaggtgatct ggatttagag aaatttctcc aaatagcgca ggatttgggt
     1441 ctctacgcaa ttgtgcgtcc gtctccattt atctgtgcgg aatgggaatt cggtggctta
     1501 ccagcttggc tcttgaccaa gaacatgcga attcgctcat ccgacccagc atatatcgag
     1561 gcagttggtc gctactatga tcagttattg ccaagactgg tgcctcgttt gttgaacaat
     1621 ggtggcaata ttctcatgat gcaggttgaa aatgagtatg gttcttacgg agaagataag
     1681 gcttacctga gagcgattcg acagctaatg gaagagtgtg gcgtaacctg tcccctcttt
     1741 acatcagatg gtccatggcg agctactctg aaagctggaa ccttaattga agaggacctc
     1801 tttgtaacag gaaactttgg ttctaaggca ccttacaact tttcgcagat gcaggaattc
     1861 tttgatgaac atggtaagaa atggccactc atgtgtatgg agttctggga tggttggttc
     1921 aatcgctgga aagaaccgat tatcacacgg gatcctaagg aattggcaga tgcagttcga
     1981 gaggttttgg aacaaggctc tatcaatctt tacatgttcc acggtggtac aaactttggt
     2041 ttcatgaatg gttgctcagc tcgaggaact ttggacctgc cacaagttac atcttatgat
     2101 tacgatgccc ttctggatga agaaggaaat ccaactgcta aatatcttgc agtcaagaag
     2161 atgatggcaa cacatttttc agagtatccg cagttggaac cactctacaa agagagtatg
     2221 gagttggatg ctattccact agttgaaaaa gtttctttgt ttgaaacctt agatagcttg
     2281 tcaagtcctg tagaaagtct ctatcctcaa aagatggagg agctgggaca aagttatggc
     2341 tacctacttt atcgaacaga aacaaactgg gatgcagaag aagaaagact tcgtatcatt
     2401 gatggtcgag atagggccca gctgtatgtc gatggtcagt gggttaaaac tcaatatcag
     2461 acagagattg gggaagatat tttttatcaa ggtaaaaaga aagggctatc taggttagat
     2521 atcttgatag aaaatatggg gcgtgtcaac tatgggcata agttcttagc ggatacgcaa
     2581 cgtaagggaa ttcggacagg ggtctgtaag gatctgcatt tcttactaaa ctggaaacac
     2641 tatccactcc cactagacaa tcctgagaaa attgattttt caaaaggatg gactcaagga
     2701 caaccagcct tttacgctta tgactttaca gtcgaagagc caaaagatac ttacctagac
     2761 ttgtctgagt ttggtaaggg agttgccttt gtcaatgggc agaatctagg acgtttttgg
     2821 aacgttggcc caactctctc actttatatc cctcatagct atctcaagga aggtgccaac
     2881 cgtatcatta tctttgaaac agaaggtcaa tataaagaag agattcattt aactcgtaaa
     2941 cctacactaa aacatataaa gggggaaaac ttatgacaat tgtaggatgc cgtattgatg
     3001 gacgtttgat ccacggacaa gtagccaatc tttgggctgg aaaactaaat gtttcacgca
     3061 ttatggttgt agacgacgaa gttgtcaaca acgatattga aaagagtggt ttgaaacttg
     3121 cgacaccacc aggtgtgaaa ttgagtattt tgccagttga gaaagctgca gccaatattc
     3181 ttgctggtaa atacgatagc caacgtctct ttatcgtggc tcgtaaacca gaccgcttcc
     3241 ttggtttggt agaagcaggt gtaccacttg aaacccttaa tgttgggaat atgtctcaaa
     3301 caccagaaac tcgttctatt acacgttcta tcaacgtagt agacaaggat gtggaagact
     3361 tccacaaact ggcagaaaaa ggtgttaaac ttactgctca gatggttcca aatgatccaa
     3421 tttcagactt tttgagctta ttaaaatagg aaaaaaattt ttaggaggtc attgttatga
     3481 tacaatggtg gcaaatttta cttctcactt tgtactcagc ttatcaaatc tgtgatgagt
     3541 tgacgatcgt ttcatctgca ggttcccctg tatttgctgg tttcattact ggtttaatca
     3601 tgggagatgt gactactggt ttacttatcg gtggtaactt gcaactgttc gttcttgggg
     3661 ttggtacctt cggtggtgct tctcgtatcg acgcaacttc tggtgcggtt cttgcgacag
     3721 ccttctctgt ttcacaagga attgatgcac cgcttgccat tactacaatc gctgtaccag
     3781 tagcagctct cttgacttac ttcgacgttc ttggtcgtat gactactacc ttcttcgctc
     3841 accgtgtgga tgctgcaatc gaacgctttg actataaagg tattgaacgc aactacttgc
     3901 ttggtgcgat tccgtgggct ctatctcgtg cccttccagt cttctttgcc cttgcttttg
     3961 gtggtgcctt tgtacaatca gtagtagact tcgttgaagc ctacaaatgg gttgcagatg
     4021 gcttgacact tgcaggacgt atgcttccag gtcttggatt tgcaatcttg cttcgttacc
     4081 ttccagttaa acgtaacctt cactaccttg ctatgggatt tggtttgaca gctatgttga
     4141 ctgttcttta ctcatatgta acaggtcttg gtggcgctgt tgctggtatc gtaggtactc
     4201 ttcctgctga agttgctgaa aaaattggtt tcgtgaacaa cttcaaaggt ttgtctatga
     4261 ttggtatttc tatcgtaggt attttccttg cagtgcttca cttcaaaaat agccaaaaag
     4321 tagctgtagc agcaccttct acaccatcag aaagtgggga aatcgaagat gacgaattct
     4381 aattacaaac ttacaaaaga agattttaat caaatcaaca aacgtagctt gtttactttc
     4441 caattaggtt ggaactacga acgtatgcaa gcttctggtt acctttacat gatcttgcct
     4501 cagttgcgta aaatgtatgg tgatggaact cctgaattga aagaaatgat gaaagttcat
     4561 actcaattct tcaatacttc accattcttc cataccatta tcgctggttt tgaccttgcc
     4621 atggaagaaa aagatggtgt aggttcaaaa gacgccgtta acggtatcaa gacaggtttg
     4681 atgggaccat tcgctcctct tggggataca atctttggtt cacttgtacc tgctatcatg
     4741 gggtcagtcg cagcaactat ggctatcgct ggccaacctt gggggatctt cctttggatt
     4801 gcagttgcag tagcgtatga catcttccgt tggaaacagt tggaatttgc ttacaaagaa
     4861 ggggttaacc ttatcaacaa catgcaaagt accttgacag ctttgattga cgctgcatct
     4921 gtacttggtg tcttcatgat gggtgctctt gtagcaacag tgattaactt tgaaatttct
     4981 tacaagttgc caatcggtga aaagatgatt gatttccaag acatcttgaa ccaaatcttc
     5041 ccacgtttgc ttccagcaat ctttactgcc tttatcttct ggttgcttgg taagaaaggt
     5101 atgaactcta ctaaagctat cggtattatt atcgtacttg ctttggctct ttctgccctt
     5161 ggtcactttg cacttggaat gtaattcctt atgactaaat cattaatttt ggtgagccat
     5221 ggtcgcttct gtgaggagct tagaggtagc acagaaatga ttatgggccc acaagacaac
     5281 atttacacag tagctcttct tccagaagat ggcccagaag aatttactgc taaatttgaa
     5341 gctgttattg aaggattgga tgatttccta gtctttgcgg atcttctcgg tgggacacct
     5401 tgtaatgtgg tgagtcgctt gatcatggaa ggtcgtgata ttgaccttta cgcagggatg
     5461 aatcttccaa tggtgattga atttatcaat gcgagcctta caggcgcaga tgcggactac
     5521 aagagccgtg ctgcagaaag cattgtgaaa gttaatgacc tgttagcggg cttcgatgat
     5581 gacgaagatg aataatactc ttcgaaaatc tcttcaaact acgtcaacgt cgccttgccg
     5641 taggtatatg ttactgactt cgtcagtctt atccggcaac ctcaaaacgg tgttttgagc
     5701 tgacttcgtc agtcttatcc ggcaacctca aagcagtgct ttgagcagcc tgcggctagt
     5761 ttcctacaga ttttagttgg aactcgattc aattcatgtg acaacgtgaa aatcgttaga
     5821 gcattttata tagaatatac atgggaatgt agcttactcc cattcccata tttaatagaa
     5881 aaagaggaac tcaatgctac attatacaaa agaagacttg ctcgaattgg gtgcagaaat
     5941 cactacgcgt gaaatctacc aacagcctga tgtatggaga gaagcttttg aattttatca
     6001 agcaaaacgt gaagaaattg cagccttcct acaagaaatc gctgataaac atgactatat
     6061 taaggttatc ttgacaggtg ctgggacttc tgcttatgtg ggagatacct tgctacctta
     6121 ttttaaggaa gtctatgacg aacgcaaatg gaatttcaat gctattgcga caacagatat
     6181 cgttgccaat ccagcaacct atttgaaaaa agatgtggca actgtccttg tgtcttttgc
     6241 tcgtagtggg aattcgcctg aaagtttggc gactgttgat ttggccaaat ccttggtgga
     6301 tgagctttat caagtgacga ttacttgtgc agcagatggt aaattggctc ttcaagctca
     6361 cggtgatgat cgtaatctct tgctcttgca accagctgtc tctaatgatg ctggatttgc
     6421 catgacttct agctttacgt ctatgatgtt gacaactctc ttggtctttg atcctacaga
     6481 atttgctgtt aagtctgaac gttttgaagt tgtatctagt cttgcccgta aagttttaga
     6541 caaggcagaa gatgtcaaag agctcgttga tttagacttt aaccgtgtca tctatctagg
     6601 cgctggtcct ttctttggac ttgctcatga agctcagctc aagattttgg aattaactgc
     6661 tggtcaagtt gcgaccatgt atgaaagccc agttggcttc cgtcacggtc caaaatctct
     6721 tatcaacgac aatacagttg ttttggtctt tggtacaacg acagactaca ctcgtaagta
     6781 cgacttggac ttggttcgtg aagttgctgg tgaccagatt gctcgtcgtg ttgtgctttt
     6841 gagtgatcaa gcttttggtc ttgaaaatgt caaagaagtg gcccttggtt gtggcggtgt
     6901 cttgaatgat atttaccgtg tcttccctta catcgtttat gcccaactct ttgctttatt
     6961 gacttcactc aaggtagaaa ataaaccaga tacaccgtct cctacaggta cagtaaaccg
     7021 tgtagtacaa ggtgtcataa ttcacgaata tcaaaagtaa gacagtgttt atgaattctt
     7081 gacaagagga tttgtaaatt atcagataaa ccatagattg tcagtacgct ttctatggtt
     7141 tgtttgcttg agagaaatag taaaaggaga acagaatgaa agcatacaca gagcgtgtat
     7201 ttggaaatgt tgagggtgag gatgtcttgg cctatcgatt tgagacagac ggtggctacc
     7261 aacttgaggt tatgacttat ggtgcgacta tcttgcgcta tgtcgcacct gacaaggctg
     7321 gaaattttgc caatgttatc ttgggatttg atgactttga tagttatgta ggcaatagtc
     7381 ccaagcatgg agcaagtgta ggtcctgtag cgggtcgtat tgcaggtgcg acctttgagc
     7441 tcaatggtaa gacctatgac cttgaggtta ataatgctag caactgtaat cacagtggtt
     7501 caactggttg ggattccagc ttgtttgaag ttgaagaagt aagcgatcat ggcttgactc
     7561 tctacacaga gcgtacagat gggacaggag ggttccctgg aaatctcaag atttggatca
     7621 gttatcactt ggaagaaact ggtgcctatg aaatcagcta caaggtaacg accgatcagg
     7681 atacgctggt caatccaacc aaccacagct atttcaactt gtctggtgat ttcacgcaga
     7741 cgattgaccg tcatgtcttc caactaaaca cagagggcat ttactcaatc gctcctgacg
     7801 gtgttcctgc caaaactcca gaagccaacc gtgatgtggt caaacacgtc tacaatggta
     7861 ccttgttgaa ggatatcttt gcagaagaag atgagcaaat ccagctggca tcaggtttgg
     7921 atcatccatt tgcccttcct gcaggccatg acaatgctgg attcctttat gaccaaaatt
     7981 caggtcgctt cctgcttttc aagacagaag ctccttgctt tgtggtctac acagcaaact
     8041 ttgtggatga aagtgtcatc ataggaggtc agccaatgct acagcacaat gggattgctc
     8101 ttgaagcgca agctttacca gatgccattc acagtgacct taaaggccaa gtcattctta
     8161 aagctggtca aaccttcacc agtaagacac gttatgaact tgttgtgaaa taaaaaaagc
     8221 tggtcaaaaa gtctttaaaa ttaaaataac gcatattgtc aggtgatgaa aagtttgata
     8281 atatgcgttt tattatgaaa tgatttactt tatattatcc tgaaatggtg ttatttttga
     8341 gttatctggc ttgactttct tgatggaaat tatataatag ttgtaacaat aataattaca
     8401 actagaaaag aggaccattt atgacctatg aatacaagag tcacatttat ttggcagaga
     8461 cagctttaaa tgtaaaggat ttggccagtc aaacagcctt ttatcagcaa gttattggtt
     8521 tagaaatctt atctcaaact gagacagagt ccattctagg ccttggtgga aaagtcttgg
     8581 tacagctgat tcaagcacaa gagagcggag aagtgaggga atattatggt ctttaccatt
     8641 tggccattct cttgcccaca cgcaaggctt tagctgatgt cttgaaacac ctgacagatt
     8701 tacaaattcc tcttgtcggc ggtgcagacc atggttacag tgaagctatt tacttggaag
     8761 atttggaggg aaatggcatt gaactctatc gagataagcc agtttccaca tgggatattc
     8821 gagaagatgg acgcattatc ggagtgacgg aggctcttgc ggcgcaggac atttatgagt
     8881 tgggggaaag agtagagccc tttatcctgg cagaaggtac aagaatgggg catattcatc
     8941 tttccgtcaa ggatagtcga aagtccagcc agttttatca aaaggtgtta gggctagaga
     9001 ataaattcag tgtgcctggc gctagttgga ttgcggctgg agattaccat catcatttag
     9061 cagttaacga atggggagga aaaggtctgg cttcgcgtaa gcaaggctta ccaggtttag
     9121 cctactatgt catcgaagtc gcacataaaa aagaactgtt aacgattgcc caacgagcac
     9181 aagaagttga cgcaccaatc aaatggatga catcgatcca attggaaatc acagactcag
     9241 atggcatcgt gacccgtatt cgtttagcaa ggtaaaatta ggaattttta ataagttata
     9301 agtattgcta gtttgctttt tgaacggtct ttggtaacag aactgcgtga gaattttgac
     9361 aaagaagctg attagagata taatgaagaa aaatcgagca aggaaaaaga actgacaaat
     9421 tcaaagtata ttacttgttt gaaacgttca gagggccagt tgtgtgggat tcaaaagatg
     9481 attgaaggag atcgtgactg tgctgatatt gtgacgcaac tgacagcagt gaaatctagt
     9541 gtagagcgcg tgattgagat gataattacc gaaaatctta ctgaatgtat caatcagccc
     9601 ctagatgatt ctgaagctca aaaggaacgc ctagaaaagg ctatccgata cttgattaaa
     9661 cggaaataaa tgaatgtttt gagacgatat gtgcaaaggt ctattaacta ttagaatagt
     9721 ttaatgtgag tcaaaagaag ttgtaaaaaa tctctaaaat taaaaaaacg catagtatca
     9781 ggtgttgaat gtactgaccc ccaaaagtta gattttttct gtctaacttt tggggtgcag
     9841 ttcataagaa ccttggtaat atgcgtttta ttatgtagag acttatactc ttcgaaaatc
     9901 tcttcaaacc acgtcagctt caccttgccg tagatatggt tactgacttc gtcagttcta
     9961 tccacaacct caaaacagtg ttttgagctg acttcgtcag ttccatccac aaccttaaaa
    10021 cagtgttttg agcagcctgc ggctaacttc ctagtttgca ctttgatttt cattgagtat
    10081 tacttcatct tttcctgaaa ttgagttttt gcccaactcc tcttgattat tctacttgac
    10141 cgggccaagc attcatacca ccttctacgt tggtaacggt gaggccttgg gagctgagaa
    10201 actggcaggt agatgcagag cggactcctc cttgacaaat gacatagtat tcatggtcag
    10261 gtttgagttc tttgtagcct tgctctaagg tacttaatgg aagatttttg gcacctggtg
    10321 catgtcctgc ttggaattca tgtgcttcac ggacatcgat aagttctaga ttttcatttt
    10381 ggtattttcc ataaaagtca gccatgctga tactagtttc catattctac tccttctggg
    10441 ttaaccattt tgtatagtga ataagcgccg tcaaggtttt gggcggtaaa tcctgcttgc
    10501 ttgaggatag gctctgcgat atagctgcgc aaaccactgt ggcagctgac gatgtaggct
    10561 tgttttttgt ccagttcatc agtttcctcc ttgacacttt gtcagttttt gatacaatag
    10621 tacaaaatta gaggaggcag gctatgattc agaaacatgc gattcctatt ttagagtttg
    10681 atgacaatcc tcaggcggtt atcatgccca atcacgaggg gctggacttg cagttgccaa
    10741 agaagtgtgt ttatgcattt ttaggtgagg agattgaccg ctatgcaagg gaagtagggg
    10801 cggattgtgt cggcgaattc gtttctgcta ccaagaccta tccagtctat gtcatcaact
    10861 acaagggtga ggaggtctgt ctggctcagg ctcctgttgg ctccgctcca gcagcccagt
    10921 ttatggattg gttgattggc tatggtgtgg agcagattat ctctactggg acctgtggtg
    10981 tcctagctga tatagaggaa aatgcctttc tagtccctgt tcgcgctttg cgagatgagg
    11041 gagccagtta ccactatgtg gcaccttgtc gttatatgga aatgcagcca gaggctattg
    11101 ctgctattga ggaagttttg gaagacagag ggattcctta tgaagaagtc atgacctgga
    11161 cgacagacgg tttttaccga gaaacggctg aaaaggtggc ttatcgtaag gaagaaggct
    11221 gtgctgttgt ggagatggag tgttctgctc ttgcggcagt agctcaattg cgtggggttc
    11281 tctggggtga attgttgttc acagcagatt ctctagcgga cttggaccag tacgacagtc
    11341 gtgactgggg ctcggaagct tttaataagg cgctagaact gagtttagca agtgttcacc
    11401 acctttagtt gtactggcaa aggatttgtt ttatcataaa atgtctagct catacttttc
    11461 aaaaatatgt ttaaacgagg tcaccttcct cttgtcctag gcatgttgag gttgggaaaa
    11521 atctttaaaa tcagaaaaac gtatcatatc aggtgatgaa aattttgata ctatgcgttt
    11581 tatgttggta agatttagag tgagatgaaa tgatactctt cgaaaatctc ttcaaaccag
    11641 gtcagcttca ccttgccgta tagatgttac tgacttcgtc agttctatct gcaacctcaa
    11701 aacagtgttt tgagctgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc
    11761 aacctgtgac tagctttcta agcgatgcct tggttctcat tgcctataat caaaaagaga
    11821 aattttctcc tgaaaagcat atagagtagc tggcgttaaa agctcctgtc ttgctttttt
    11881 gacctatagt cacatctatc aagtattgtt cttgcctaag ctatcaataa aaaggtggca
    11941 ttttttaggc ttggtgttag tgaattttgc cttatcctat ctaagtcatt tcgagctttt
    12001 tatggtacaa tggaaacatg ttattcaaat tatctaagga aaaaatagag ctaggcttat
    12061 ctcgtttatc gccagcccgt cgtatttttt tgagttttgc cttggtcatt ttactaggct
    12121 ctcttctttt gagcttgccc tttgtccaag ttgaaagctc acgagcgact tattttgatc
    12181 atcttttcac tgctgtctct gcagtctgtg tgacaggtct ctcaaccctt ccagtagctc
    12241 acacctataa tatatggggc caaataatct gtttgctctt gattcagatc ggtggtctag
    12301 ggctcatgac ctttattggg gttttctata tccagagcaa gcaaaagctt agtcttcgta
    12361 gccgtgcaac tattcaggat agttttagtt atggagaaac tcgatctttg agaaagtttg
    12421 tctattctat ttttctcacg acctttttgg ttgagagctt gggagctatt ttgcttagtt
    12481 ttcgccttat tcctcaactt ggctggggac gtggtctttt tagttccatt tttctagcga
    12541 tctcagcctt ctgtaatgcc ggttttgata atttagggag caccagttta tttgcttttc
    12601 agaccgattt actggtcaat ctggtgattg caggcttgat tattacaggc ggccttggtt
    12661 ttatggtctg gtttgatttg gctggtcatg taggaagaaa gaaaaaagga cgtctgcact
    12721 ttcatacgaa gcttgtacta ttgttgacta taggtttgtt gttatttgga acggcaacta
    12781 ctctctttct tgagtggaac aatgctggaa cgattggcaa tctccctgtt gccgataagg
    12841 ttttagttag cttttttcaa acagtgacga tgcgaacagc tggcttttct acgatagatt
    12901 atactcaggc tcatcctgtg actcttttga tttatatctt acagatgttt ctaggtgggg
    12961 cacctggagg aacagctggg ggactcaaga ttacgacatt ttttgtcctc ttggtctttg
    13021 cacgaagtga gcttctaggc ttgcctcatg ccaatgttgc gagacgaacg attgcgccgc
    13081 gaacggttca aaaatccttt agtgtcttta ttatcttttt gatgagcttc ttgataggat
    13141 tgattctgct agggataaca gccaaaggca atcctccctt tatccacctc atatttgaaa
    13201 ccatttcagc tcttagtaca gttggtgtaa cggcaaatct gactcctgac cttgggaaat
    13261 tggctctcag tgttatcatg ccacttatgt ttatgggacg aattggtccc ttgaccttgt
    13321 ttgttagctt ggcagattac catccagaaa agaaagatat gattcactat atgaaagcag
    13381 atattagtat tggttaagaa aggaaagagc atgtcagatc gtacgattgg aattttgggc
    13441 ttgggaattt ttgggagcag tgtcctagct gccctagcca agcaggatat gaatattatc
    13501 gctattgatg accacgcaga gcgcatcaat cagtttgagc cagttttggc gcgtggagtg
    13561 attggtgaca tcacagatga agaattattg agatcagcag ggattgatac ctgcgatacc
    13621 gttgtagtcg cgacaggtga aaatctggag tcgagtgtgc ttgcggttat gcactgtaag
    13681 agtttggggg taccgactgt tattgctaag gtcaaaagtc agaccgctaa gaaagtgcta
    13741 gaaaagattg gagctgactc ggttatctcg ccagagtatg aaatgggaca gtctctagca
    13801 cagaccattc ttttccataa tagtgttgat gtctttcagt tggataaaaa tgtgtctatc
    13861 gtggagatga aaattcctca gtcttgggca ggtcaaagtc tgagtaaatt agacctccgt
    13921 ggcaaataca atctgaatat tttgggtttc cgagagcagg aaaattcccc attggatgtt
    13981 gaatttggac cagatgacct cttgaaagca gatacctata ttttggcagt catcaacaac
    14041 cagtatttgg ataccctagt agcattgaat tcgtaaagag ggatgacccc tcttttttga
    14101 tgcccaagat ggcaaataga gacagaagcc ccttgtcttc tagtaaaagt tcttcaaagg
    14161 ctggacttta tggtaaaata gaaggaagtg acaagagaga gtaatactca atgaaaatca
    14221 aagatcaaac taggaaacta gctgcgggct gctcaaaaca ctgttttgag gttgtggata
    14281 gaactgacga agtcagctca aaacactgtt ttgaggttgc agatagaact gacgaagtca
    14341 gtaacatcta tacggcaagg cgacgttgac gcggtttgaa gagattttcg aagagtataa
    14401 gaaaaaatca gttccctaaa ggagcagatt atgaagttat tgtctatcgc tatttctagc
    14461 tataatgcag cagcctatct tcattactgt gtggagtcgc tagtgattgg tggtgagcaa
    14521 gttgggattt tgattatcaa tgacgggtct caggaccaga ctcaggaaat cgctgagtgt
    14581 ttagctagca agtatcctaa tatcgttaga gccatctatc aggaaaataa aggacatggc
    14641 ggtgtggtca atcgcggctt ggcagaggct tctgggcgct attttaaagt agttgacagt
    14701 gatgactggt ggatcctcgt gcctacttga aaattcttga aaccttgcag gaacttgaga
    14761 gcaagggtca agagatggat gtctttgtga ccaattttgt ctatgaaaag gaaggtcagt
    14821 ctcgtaagaa gagtatgagt tacgagtcag tcttgcctgt tcggcagatt tttggctggg
    14881 accaggtcgg aaatttctcc aaaggccagt atatcatgat gcactcgctg atttatcgga
    14941 cagctttgtt gcgtgctagc cagttctaac tgcctgaaca tactttttat gtcgataatc
    15001 tctttgtctt tacgcccctt cagcaggtca agaccatgta ctatctgcct gtcgatttct
    15061 atcgttattt gattgggcgt gaggaccagt ctgtcaatga gcaagtgatg attaagtgca
    15121 ttgaccagca actcaaggtc aatcgactct tggtagacca gcttgatttg tcccaagtga
    15181 gtcatcccaa aatgcgagaa tatctgctga atcatattga aatcacgacg gtgatttcca
    15241 gtaccctgct caaccgatct gaaacagcgg agcatctggc aaaaaaacgc caattgtgga
    15301 cctatattca gcagaaaaat ccagaagtct ttcaggctat tcgtaagacc atgttgagcc
    15361 gtttgaccaa acattctgtc ttgccagatc gcaaactgtc caatgtcgtc tatcaaatca
    15421 ccaaatctgt ttatggattt aattaatata agtgttttat aagagggatt taagaaaaat
    15481 tttaactttt tcttagtcct ttttaatttc aggagattat actagagtca tcaaataaag
    15541 aaagactcta aggagaatcc tatgaaattc aatccaaatc aaagatatac tcgttggtct
    15601 attcgccgtc tcagtgtcgg tgttgcctca gttgttgtgg ctagtggctt ctttgtccta
    15661 gttggtcagc caagttctgt acgtgccgat gtggtcaatc cgaccccagg tcaagtctta
    15721 cctgaagaga catcgggaac gaaagagggt gacttatcag aaaaaccagg agacaccgtt
    15781 ctcactcaag cgaaacctga gggcgttact ggaaatacga attcacttcc gacacctaca
    15841 gaaagaactg aagtgagcga ggaaacaaac tcttctagtc tggatacact ttttgaaaaa
    15901 gatgaagaag ctcaaaaaaa tccagagcta acagatgtct taaaagaaac tgtagataca
    15961 gctgatgtgg atgggacaca agcaagtcta gcagaaacta ctcctgaaca agtaaaaggt
    16021 ggagtgaaag aaaatacaaa agacagcatc gatgttcctg ctgcttatct tgaaaaagct
    16081 gaagggaaag gtcctttcac tgccggtgta aaccaagtaa ttccttatga actattcgct
    16141 ggtgatggta tgttaactcg tctattacta aaagcttcgg ataatgctcc ttggtctgac
    16201 aatggtactg ctaaaaatcc tgctttacct cctcttgaag gattaacaaa agggaaatac
    16261 ttctatgaag tagacttaaa tggcaatact gttggtaaac aaggtcaagc tttaattgat
    16321 caacttcgcg ctaatggtac tcaaacttat aaagctactg ttaaagttta cggaaataaa
    16381 gacggtaaag ctgacttgac taatctagtt gctactaaaa atgtagacat caacatcaat
    16441 ggattagttg ctaaagaaac tgtcgaaaaa gctgttaaag ataacgttaa
//
LOCUS       DAVKLW010000045        14453 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKLW010000045 DAVKLW010000000
VERSION     DAVKLW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.045"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(122..1972)
                     /locus_tag="VW324_001517"
     CDS             complement(122..1972)
                     /locus_tag="VW324_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9727126.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2014..3171)
                     /locus_tag="VW324_001518"
     CDS             complement(2014..3171)
                     /locus_tag="VW324_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9727127.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNML"
     gene            3229..4395
                     /locus_tag="VW324_001519"
     CDS             3229..4395
                     /locus_tag="VW324_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9727128.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="VW324_001520"
     CDS             4534..4860
                     /locus_tag="VW324_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9727129.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="VW324_001521"
     CDS             4847..5440
                     /locus_tag="VW324_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9727130.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="VW324_001522"
     CDS             5433..6344
                     /locus_tag="VW324_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9727131.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6406..7470
                     /locus_tag="VW324_001523"
     CDS             6406..7470
                     /locus_tag="VW324_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727132.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAAESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7541..8401)
                     /locus_tag="VW324_001524"
     CDS             complement(7541..8401)
                     /locus_tag="VW324_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9727133.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWRIIKLFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAHLTDNAVRFTICQGIFNPIFFSHEDLKSFLEEDGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGQVHGVYHLAQGVKTVLPREPFVPAAYMEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(8523..9509)
                     /locus_tag="VW324_001525"
     CDS             complement(8523..9509)
                     /locus_tag="VW324_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9727134.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9781..11256)
                     /locus_tag="VW324_001526"
     CDS             complement(9781..11256)
                     /locus_tag="VW324_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9727135.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11514..12437)
                     /locus_tag="VW324_001527"
     CDS             complement(11514..12437)
                     /locus_tag="VW324_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9727136.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12451..13380)
                     /locus_tag="VW324_001528"
     CDS             complement(12451..13380)
                     /locus_tag="VW324_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9727137.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13699..13920)
                     /locus_tag="VW324_001529"
     CDS             complement(13699..13920)
                     /locus_tag="VW324_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727138.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(13874..14134)
                     /locus_tag="VW324_001530"
     CDS             complement(13874..14134)
                     /locus_tag="VW324_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727139.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            14214..14351
                     /locus_tag="VW324_001531"
                     /pseudo
     CDS             14214..14351
                     /locus_tag="VW324_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4334 a         3088 c         2666 g         4365 t
ORIGIN      
        1 aaccatatac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtataagtc
       61 tctacataat aaaacgcata ttaccaaggt tgtgtaactt gatactatgc gtttttcttt
      121 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
      181 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
      241 ccttaccaac gaaaatctta tccataactt gattatcaac gagttcggtt gataccaaga
      301 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
      361 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
      421 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
      481 ggctagcttc tggaatggtc ataaaataac gggtcatgcg gaagtctgtc accgttacag
      541 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
      601 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
      661 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
      721 tcgaaatcat aaccatctta gacactttag cttcatcaac agccttagca acattgtaag
      781 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
      841 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
      901 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
      961 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt
     1021 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
     1081 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     1141 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     1201 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     1261 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     1321 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     1381 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     1441 attcactggt tggatgttgg taactatcca taaaaagagc cccaccatca ccggcaccaa
     1501 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     1561 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     1621 tgaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     1681 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatgc
     1741 tcgcgttcaa cccccaaaaa ccaatcatca attgatagaa caggaaggcc aaactcgtat
     1801 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     1861 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     1921 tatttaaaat ttccaccaga tcaatcacat aatctgttag ttttttattc atagaaatct
     1981 actcctcctt aaaagagttt tactatattg ttattatagc atattttgat ttatttggct
     2041 tattttatcc aaataaatta ttgttccctt gtactccttc ttccatttaa atcctctaat
     2101 taaacctaca atcgtcccaa agccataagc aaagtgaatg gaaaataaaa gaaagggcat
     2161 cacaattaga aatccatttt tatgttttaa taaagtcagc aaagtgagta gtgacaaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacatag tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatgt tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac attccatgaa aaatagaaga
     2581 aacatatcta tcctcggaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct tcgacaatcg tcggtctagg
     2701 ccccccacag acaaattcac cttgttgaat aatagccaca ttgttcatta caaaagtctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggtcc cctacagaat gtttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgtatct tcctttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tattctcttt aggataagtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaatttttcc ttccaccatt ctatcataat
     3121 tttcaagact ttcccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga ggtattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccaa
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctactctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat ccttctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgtcat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gtgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctgttcc actcttgggt accatctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatgggca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagcggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaattctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgcaa ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg caaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc
     6361 tgctactatg gaagcatatt tatctaacca aaaggaggtt ctaccatgaa acaagaatgg
     6421 tttgaaagta atgattttgt aaaaacaaca agcaagaaca agcctgaaga gcaagctcaa
     6481 gaggttgcag acaaggctga agaaacgata gccgatctcg atacaccaat tgaaaaaaat
     6541 actcagttag aggaggaagt ctctcaagct gaagtcgaat tggaaagcca gcaagaagag
     6601 aaaattgaag ctcctgaaga cagtgaagcg agaacagaaa tagaagaaaa gaaggcatct
     6661 aattctactg aagaagagcc agacctttct aaagaaacag aaaaagtcac tatagctgca
     6721 gagagccaag aagctcttcc tcagcaaaaa gcaaccacga aagagccact tcttatcagt
     6781 aaatctttag aaagtcctta tatccccgac caagctccaa aatctaggga taaatggaaa
     6841 gagcaagtgc ttgatttttg gtcttggcta gtggaagcga tcaaatctcc tacaagtaag
     6901 ttggaaacaa gtatcacaca cagttacaca gcctttctct tgctcattct gttttctgca
     6961 tcttcctttt tctttagtat ctatcacatc aaacatgctt actatggaca tatagcaagc
     7021 attaacagtc gcttccctga gcagctagct cctttaactc ttttttctat catctctatc
     7081 ctagtagcga caacactctt cttcttttca ttcctcttgg gtagtttcgt tgtgagacga
     7141 tttatccacc aggaaaagga ctggacgcta gacaaggttc tccaacaata tagtcaactc
     7201 ttggcaattc caatcttcct cactgctatt gctagtttct ttgctttctt tgatagccta
     7261 cgatttacag ccctcttgtg tgtgattagc attggaatca ttctgcttgc cagtctccat
     7321 atcattacaa gaccaagtca agcaagtgaa accgactcct tctatcaatt attcttgtct
     7381 gtccttgtga acggagttat tatcctcctc ttctttgtag ctgaagtggc actgattgga
     7441 gattatcttc gtatcttggc ctttctttaa aattcaatcc tgtcatcctt ggcaggattt
     7501 ttctatttca aaaaagcaag tcgattgacc tgcttctgct ttacttttct tgtgctaatg
     7561 ttttaaaatc ttgtcctagg atgggttgta tttctatttg attggcgtct agttggtggt
     7621 aaaaagatgg gggtaatacc tctaagaatt ttgcatagtc caagcgagct atggattcat
     7681 agtcttctgg tttagagccg tctcctgtga tttgaaccaa gtcaatctcc cactggactt
     7741 ccttatccac caattgctcc atataggcag caggaacaaa tggttctctc ggtaaaacgg
     7801 tcttgacgcc ttgagccaaa tgataaacac cgtgcacttg cccttctcca tcctgataga
     7861 aggaaactct tgcaaagtag gcatctgtct cttgaaccgc acgcacacgc gcttcaaact
     7921 gagccaaacc atcttcctct aaaaagcttt tcaagtcctc atgactaaag aaaataggat
     7981 taaaaattcc ttggcaaatc gtaaaacgaa ctgcattatc tgtcagatgg gcttgaatac
     8041 ttgcttggaa ataagcttca aaatcaaaac catctagtcc ttcaatatca gtccctgtat
     8101 aagcaagcag agcatctaaa aataacttga taatccgcca gtctgtcttc gtgagtaggt
     8161 caggaagatc gacacggtaa gccttttgac catcagcata actgacctta aaaagaaatt
     8221 gtgattgccc cactatcgca cattcgatat actcgagacg attaagaggc tgacggagat
     8281 agactacatc atagctatgt gactccaaac cgtctagcaa ggccaagatc gacttggcat
     8341 tcaaaatctc ttgttctcct aaaatgctct gtttatttgg aataaaaaat gttttcgcca
     8401 taactggact cctttgaaat cgtttacata cagtatacct tatttttctg aaagatacaa
     8461 aaacaaactt tagatttttg agtacaaagc atagaaaaac agcccctgag gactgtttga
     8521 tcttatacag tagctgcctg atccaagctt tcaccgatag cggctaggcg ctcaatcact
     8581 tcagcttgtg tcaattcatt ttttgaaaca tagcggttac gtgggtgaac acggcactcg
     8641 tgtgagcatc cacgaaggta cttgtcttca ttttcttctg atgtcaagat acgacggtta
     8701 cagaagggat ttccacagtt aacataacgt tcacatggtg ttccatcaaa ccagtctttc
     8761 cctacgatgg ttgggttgac atggttgaca tcaacggcga tacgctcgtc aaatacgtac
     8821 atttttccat cccaaagctc accttgaact tctgggtctt taccgtaagt tgcgattcct
     8881 ccgtgcaatt ggccgacatc tttgtagcct tcacggacca tccagcctga gaatttctca
     8941 cagcgaacgc cacctgtaca gtaaaccacg acacgcttgt ccatgaattt ttccttgtta
     9001 tcacggaccc attgtggtaa ctcacggaag ttgcgaatat ctgggcgaat agctccacgg
     9061 aaatgtccta ggtcgtactc ataatcgtta cgtgtgtcaa ggacaacggt atctttatca
     9121 agaagcgctt ctttgaactc ttttggagac aagtaagcac ctgttgtttc aagtgggttg
     9181 atgtcattgt caaagtcgtt gtcttccaaa ccaaggtgga caatttcttt cttgtagcga
     9241 acaaacatct tcttgaaggc ttgttcattt tcttcgtcaa tcttgaacca gagttcttcc
     9301 attcctggaa ggctgtgaac gtagtccatg tatttttgag ttgtttcata gtcacctgaa
     9361 actgttccgt taattccctc gtcagcgact aggatacggc ctttaaggcc gattgattta
     9421 cagaaagcca agtggtctgc agcaaattgc tctgcatttt caattggagt ataaaggtag
     9481 taaagtaaga cacgaatatc ttttgccata agatttgttc tcttttctat tcttaaattt
     9541 tctaaatttt ttatttatct acactagtat acctgcttga gaaaacgaat gcaatttatt
     9601 tgactggaaa ttgtagaggg ttctcgaaat ttcctgaatg gttaaaataa ggacaagaga
     9661 aaacatggat atctatatcc ttgtgccaaa aaaaccactg ccctccccag accaacctga
     9721 ggaaagcagt gattcttatt ttaggagtta ggaatgaata cacgaaatca atttagctga
     9781 ttattttttg tttttcaaga attcatcgta ttgtttttgc atttcgttca atactttttc
     9841 gtaggcacct tcagatttca atttttccat caattctgga atcgctttat ctgggtctac
     9901 agtaccagtg ttgatagctg tatcaaattg ttgcattgtg ttagcaatag ctgagatttc
     9961 agatttcaca ttgtcagtat tgaagataaa tccaagcgct ggagattctt tagcttctgc
    10021 caattctttc ttagaatttt cgatttgttg gtctgtaacg ttttcgttga tgtaaaggat
    10081 ccagttgtta ccagtgttcc atccacccat gtgagtgttt cctttgtagc catcaagaac
    10141 gcgaacacgg ttttctttac cttcaatttt ttcccagttc ttgccttctg gaccgtaaac
    10201 aagaccgttc aagagttctg gattcgtatt caagaggttc aagatttcca ttgatttttc
    10261 tttgttctta gagttgtttg agatgacaaa gttagcaact tgtgttgttt ggtttttctt
    10321 gatgaagtta gtaattggtt tgatttggat atctttgttg gcaacacgtg aaagcaagct
    10381 gttaccgtag tcagctggtc ctactgtttc ttcacgaacg aaccaagtat cttgttgaag
    10441 gtcaaaggaa gtatcgcttg ttgcgacgtc ttttggaatg tagccagctt catagaattt
    10501 gtgaagagtc ttcaagtgtt ctttgaaacg aggcacttcg taacggttta caactttagt
    10561 agtatcgcct tcaaggtcga taacgaatgg aagaccgttt gctactgggt agtcaaagtt
    10621 atcagatggg atgaaaactt taccaatagc aaatggtact acgtctggag ctttttcttt
    10681 gatttgtttc aagactggct caagagtttc gtaagaagta acacctgaaa tatcgatacc
    10741 atatttagca aggagagttc cgttgaaggc aaagttttga gatgatgcaa cgttggctgc
    10801 aactggaaca gcgtaaatct taccatttac agtattaccc ttgatgtaag ctgggtcaag
    10861 tgctttgtaa aggtctttac cttctttttt gtacaattct gtcaagtcag cgtaagcacc
    10921 tttttgagca tttacaatat agttatctgc aaaggcaata tcatagtttt caccagatga
    10981 tgtgataact gacattttct taccatagtc accccagcca aggtattgga tatccaattt
    11041 ggcaccaact ttttcttcaa tgattttgtt ggcatttgct aacaattcat ccaagttgtc
    11101 tggtttgtca ccgatttggt acattttgat aacaggtttg tcacctgaat cagcagcttt
    11161 tttgctgtta cctgtcaaat ttccacaagc agcaagacct gcagccaaag cgactacact
    11221 agcagatgca aaagcatatt ttttccagtt tttcatgata aaaactcctt tttttatttt
    11281 taaacttata aacaatgtaa tgatcttata ctcaatgaaa atcaaagagc aaactagaaa
    11341 actagccgca ggctgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag
    11401 ttacatatat ctacggcaag gcgacgttga cgcggtttga atttgatttt cgaagagtat
    11461 taacttcaca caagggaagt tgggaactga gaaatgttct ttttcaataa gcactattct
    11521 ttcacaccac cgatagtcaa accttttaca aagtagcgtt ggaagaatgg atataaaatc
    11581 gcgattggaa gggttgcaac cacaaccatg gccatacgac ctgtttcttt cggtagagca
    11641 actcccagtt gaccagtcaa gccgaccgct ttggcaatgt agtccatatt ttgttggatt
    11701 tgcatgagca aatattgcaa tggatacaag ttatcactct tgatgtaaag aagggcgttg
    11761 aaccagtcat tccagaaacc aagagctgtc aaaagcgtga tggttgcgat acctggtagt
    11821 gacaatggca aacagatttg gaagaaaatc cgggcctcac tggcaccatc gatacgagcc
    11881 gattcgagaa tggcttctgg aatggtcttc ttgaagaagg aacgcatcaa gatgatattg
    11941 aatggtgaga gaagcattgg aacaatcaag gcccaaacag tgtcaccaag ctgaagtaca
    12001 cgggtcacca tgatataacc tggtaccaaa ccagcgttga acaacatact gagaaggacg
    12061 aagatggtaa aaaatctgcg atacttaaag gttgtccgtg aaatagcgta ggcataggtt
    12121 gttgtgataa agacatttgt caatgtccca actacggtta caaagacaga gatgaagagg
    12181 gcttgtagga ttttatcctt aaactgtgcc aaaaactcaa aaccgtctaa gccaaattgg
    12241 gatgggaaga agctatagcc gtattggagg aggcttttct cgtctgtcac tgaaataatg
    12301 ataacgaata caaaaggtag gatacaagag agggcaatca aacccgaaat gatactgaag
    12361 aagatatctg ctttcttact gaaggagtga atgccgacat tatcaatttt ttctttttta
    12421 attttctttt ttgccatatt ctcctccttt ctagaacaaa gctgagtttg gatcgactcg
    12481 tcttgcaagc aagtttgata ggataaccag aatcaaacca acaacggatt ggtaaagacc
    12541 ggctgctgca gccataccga tatctgctgt ctgagtcaaa ccattaaaaa catatacgtc
    12601 caaaacgttg gttacattgt aaagctgacc agcattgtgt gggatttgat agaagagacc
    12661 gaagtctgcg cggaagatat ttccgactgc aaggatggtc aatacagtta caagcggagt
    12721 caactgagga atggttacgt tgcgaatacg ttgccacttg ctagctccgt ccactgtcgc
    12781 tgcttcgtag taggttggat caattcccat gatcgtcgca tagtacatga cactgctata
    12841 tccaaaacct ttccaaatac ctaggaaaag taggagatag ggccagatgc ccaggtcagc
    12901 gtagaaattg acttctttga gaccaagact ttccaataga tgattgaaca cccctttatc
    12961 aatatttagg aaggcatctg taaagaaact gatgataacc caagacaaga agtaagggaa
    13021 caacatagaa gtttggaaaa tcttcaccat tctcttagaa cggagctcgc tgaggataat
    13081 ggcaatccct acagatacaa ctaaacctag aaagataaag ccaagattgt agaggacagt
    13141 atttcgtgtg ataataaagg cgtctcttga actaaataag aatctgaaat tatcgagtcc
    13201 gacccattta ctatttatga tactatctat gaaaccatta ctggtcatgt ggtagtcttt
    13261 gaaggcaacc acgttcccaa atactggaat gtaaaagaat agaatcaacc agagtgcccc
    13321 tggcaaaacc atcaagagaa agatccagtt gtctctcaat gtttttgaaa actttttcat
    13381 aatttcctcc ctttttattt tgatatccat ctaaaaattc ttttttagac ttttgataac
    13441 gattacatta ttagtatact cctatttgca ggttaggtta aactcctaat tatagaaaaa
    13501 actccacaaa ttatgtagca gatttaaaac tttatcacca ctatcaaaca aatgtcctaa
    13561 atcaattgtt tattttatct ctattagccc agtgatggcg tcactctgtt ataagcatcc
    13621 aacaacgggg tatactgaaa aatctccaga ctagggaact cagagatagt tcctaatctg
    13681 gagattttta atatgttatt aggcgtttgc tttcaactta gcaataacct ctttaagatt
    13741 atcaatcacc tctgcttcag tatgctcaga gcctttttca tctgccaaga acaaaactgc
    13801 tttttgaagt tctttttgag agttttcaag gacatcctta tctactgttt caaggtttga
    13861 gtctttaaga agtttactta attccttggc taatttcttg agtttgattt gcagactcat
    13921 cttctcctgc tgtttctttg cccgctgttt gtcctccatc cttagttgct gactggcttt
    13981 ccttaatgga ctctagggaa gcaatggcat ctttgactgt ttgcaagata tcacgtaaac
    14041 cttgctctgt caaactatca tctgcaaaag ctttcttagc ctctgccaaa accagacgtg
    14101 ctgaatctgt ggtaggattc gatacacctg tcaatgatct caaaagattt tctaaggttt
    14161 gagtctgctt actaatacta gactaaaatc aaaaagtatt atataacagt gatatgaaat
    14221 caactaaaga agaaatccaa accatcaaaa cacttttaaa agactctcgt acagctaaat
    14281 atcataaacg ccttcaaatc gttctatttt gtctgatggg caaatcttat aaagagatta
    14341 tagaactttt atagtagttt gaaataagat gtgaacatct ctatcaggaa agtcaaatta
    14401 atttatagaa atattttagc agccaaggtg tactgttata gattcaatac act
//
LOCUS       DAVKLW010000046        14332 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKLW010000046 DAVKLW010000000
VERSION     DAVKLW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.046"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(61..1350)
                     /locus_tag="VW324_001532"
     CDS             complement(61..1350)
                     /locus_tag="VW324_001532"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9727140.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1428..2675)
                     /locus_tag="VW324_001533"
     CDS             complement(1428..2675)
                     /locus_tag="VW324_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9727141.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2866..3717)
                     /gene="rgg"
                     /locus_tag="VW324_001534"
     CDS             complement(2866..3717)
                     /gene="rgg"
                     /locus_tag="VW324_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9727142.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3841..4182
                     /locus_tag="VW324_001535"
     CDS             3841..4182
                     /locus_tag="VW324_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9727143.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            complement(4391..6094)
                     /gene="ilvD"
                     /locus_tag="VW324_001536"
     CDS             complement(4391..6094)
                     /gene="ilvD"
                     /locus_tag="VW324_001536"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9727144.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAEATLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6327..7259)
                     /locus_tag="VW324_001537"
     CDS             complement(6327..7259)
                     /locus_tag="VW324_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9727145.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7256..8113)
                     /locus_tag="VW324_001538"
     CDS             complement(7256..8113)
                     /locus_tag="VW324_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9727146.1"
                     /translation="MILSENREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8117..9463)
                     /locus_tag="VW324_001539"
     CDS             complement(8117..9463)
                     /locus_tag="VW324_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9727147.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(9476..9760)
                     /locus_tag="VW324_001540"
     CDS             complement(9476..9760)
                     /locus_tag="VW324_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9727148.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9764..11794)
                     /locus_tag="VW324_001541"
     CDS             complement(9764..11794)
                     /locus_tag="VW324_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9727149.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            11985..12992
                     /locus_tag="VW324_001542"
     CDS             11985..12992
                     /locus_tag="VW324_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9727150.1"
                     /translation="MTEKLINSKPNGVFALILIELTIILGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            12995..13195
                     /locus_tag="VW324_001543"
     CDS             12995..13195
                     /locus_tag="VW324_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9727151.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13347..13529
                     /gene="rpmF"
                     /locus_tag="VW324_001544"
     CDS             13347..13529
                     /gene="rpmF"
                     /locus_tag="VW324_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9727152.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            13545..13694
                     /gene="rpmG"
                     /locus_tag="VW324_001545"
     CDS             13545..13694
                     /gene="rpmG"
                     /locus_tag="VW324_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9727153.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14186..>14332)
                     /locus_tag="VW324_001546"
     CDS             complement(14186..>14332)
                     /locus_tag="VW324_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727154.1"
                     /translation="YLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANG
                     EWVG"
BASE COUNT         4618 a         3037 c         2457 g         4220 t
ORIGIN      
        1 caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgc tctttgattt
       61 tcattgagta taaaatccta gtttttcaaa gatttctgag aagttttggc tgattgtctc
      121 aagtgacact tgcacttctt ctcgggtttg gttgttcttg accgtcactt gtccgctttc
      181 gacttcgctc tctcctaggg tgatgagggt cttagccgca aagacatctg ctgacttgaa
      241 ctgagctttg agcttacggt tgaggtaatc acgctctgct ttgaaacctt gttggcgaag
      301 agcttggact agttctaggg ctttgacatt tgctccatca cccaagactg cgatatagac
      361 atctagggcg ttttcgatag ggagggccac gccttgtttt tcaaggatga gaagcaggcg
      421 ctctacacca agcccaaaac caaatccagc agtttcaggg cctccaaagt aagcaaccaa
      481 accatcgtag cgaccacccg cacagactgt caagtcattg ccctcaatct ctgtgataaa
      541 ctcgaaaatg gtgtggttgt agtagtccag accacgcacc atattggtat cgatgatgta
      601 atctactcca agattttcca acatctgacg cacagcatca aaatgagttt ggctttcttc
      661 atcaagaaag tccaagatag acggcgcatt ctctactgcc accttgtctt ctttttcctt
      721 agagtccaag acacgaagag gattttcctc caagcgacgt tggctatcct tagacaaggt
      781 ctccttgagc ggtgtcaaat agtcaatcaa ggcttggcgg taggctgcac ggctctcagg
      841 atttccaaga gtgttgaggt gcaatttgac accttgaata ccgatttcct tcaaaaaatg
      901 ggctgccata gcgattgttt ccacatcggt agctggattg ctagagccaa aacactcaac
      961 accaatctgg tggaattggc gcaagcgccc tgcctgtgga cgctcataac ggaacatagg
     1021 tcccatatag tagaacttgc ttggcttttg cacttctggg gcgaaaagtt tattttccac
     1081 ataggaacgg acaacgggtg cagttccttc tggacggagg gtaatatgac ggtcaccctt
     1141 gtcataaaaa tcgtacattt ccttggttac gatatccgtt gtatctccga cagagcgact
     1201 gataacctcg taatgctcaa aaataggcgt gcgcacttct gcatagttat agcgcttgaa
     1261 aatctcacgg gcaaagccct caacgtactg ccacttagca gactcagcag gtaaaatatc
     1321 ctgcgttcct tttggttttt gtaatttcat agggaatcct ctttaaactt aatagtctta
     1381 ttttaccaca aataaaggga ttaaaacagt gagaaaaaaa ttaggattta gatatcattt
     1441 ttgagattaa gaattgtcaa aaaaatagct agcaaggaaa gaccaacaaa tagcatccaa
     1501 gtcaactgta tattccatac ggctactagt gaaaaacaag ctgttcccac aggtatggat
     1561 aaggtaaaca atagacctaa aaaattacta gtacgagcta gaacctctgg agctagattt
     1621 ttcatgagca tggcactaat ctttggttga actttaccag acacatacag agtaaagaag
     1681 agaaatagca aaccaagcac gacttgattg aataaattag ccaaaccaac tagactaagt
     1741 cctacggtcg cccacatcat caatctaggc aaggactgct tcccaaaata atcattgccc
     1801 gtaaggctac tgatgataac tgctactaaa acacagaatt gattgataaa tagtgcctct
     1861 gtataagaaa aattcaagag agaatggctc aaaaagaaga tattataaat tccacccaaa
     1921 gcgccaccca aggaattaat aagcaagaca gcaaagagca taaaaccaaa gtttttctgt
     1981 ccacttttaa gaaaaacgag acgtaaattt cggtaaattg ttaggaactg gtctttgata
     2041 gaaagcttct cattttttaa gttttcacca tcagcagatg acattgacag gctcaatttg
     2101 ctttttccta aaaagaggat agtggctgat actaggaaaa agcaggcatt gattcccgca
     2161 acgagagaaa aattgttgac cgatagagct aagagccaga ctccgaaagc ttgaccacca
     2221 atagctgaaa tataggtgat gaactgtgaa aaagaataag cctccatcag atcatcttca
     2281 gctacttttt ccttaataag aggcatacgc aggccacctg caaaatcact gatgatatca
     2341 ctaatgacat tgatcaaaca caggctagaa aaggcaaaga gactagcttg ctgaacaact
     2401 agggctgcta gaaaaaatag aaccgcctga aacaaaccgc tatagaccat ccatttgacc
     2461 ttgtccctcg tgtaatctgc ccgaatccct gcaaaaactg taaagagggt cggaagaatc
     2521 atgacaatat tcgccatagc aacagcaaaa gatgcttgtg acaaggtcga tgcatagacg
     2581 ataaagacca ggttgaaaat cgaagcacca aaagcattga agaagcgtga taaagtcaaa
     2641 attctataag ctggatttct aaacaatagt ttcatagata atctccctcc ttgttctaag
     2701 ttctacatca agaatagctt tagtatagca cttatgaatt tgcgaaacga ttcttttgaa
     2761 tatatgcaac aaacaaaatg agcaaaaaaa cgatagaact tttgtaatga cctgagagag
     2821 ggcaacagtt ctatcgcttc aaattttttc ttggtttgca gatattcaac attcggtcgt
     2881 atttctatag tattcggcag atttattaca gccaagcatc tcaaaaatac ggacagcatc
     2941 ctccatcttt ttctggcctt ccttgactct accttgcttg ctatcaagga gaccttctgc
     3001 ccacagataa acaattcgga aataggtctc attttccttg tagaaatgct cttcgataac
     3061 acgtttaaaa taataggcat tggtaaattc ttcacactca atactagcta aaaagccatt
     3121 caatagtata gtatgaaaaa ggtttcgatt gccagacatt tccattagaa aatcagattt
     3181 acgtaccatt tctcgtacat atctagtaaa aagagaaaca gataaaaatg gagaactgac
     3241 tgaaaataaa ttgagttcat agattcccca gatctcggta gaaaacaaat aatcatgaag
     3301 gacttttcct tcctctgctg ttaactctac cctttcatcc atgctcttca tataagactt
     3361 gatcataatg gcatttagaa tatgtttctg tttgttgtga gaatgggcat gcttttgata
     3421 ctccctgcga tataagtcct caagaggtgc tatattcttt ggttccaaga catctgtaat
     3481 ttcttttctc aactcagaat ctgtatcata ctggaaacct ctcgccagaa agaggatctc
     3541 ctccacactg gcagatatat tttccagagc aaatagaaac ttttccaccg aaagctcact
     3601 ctgacctgtt tcaaaacggg acaacataga cggcgaaaat tgtcctccgg ttgcttgtct
     3661 cagtgagata tttcttgact ctcgtaattg tctaaagact tttccaatct gctccataga
     3721 cttccccttg attccgtatt ttcttcattt tatcatattt ttcagaaaat tcatcaaaaa
     3781 cttgccaaat tgtcagaatt atgagaaaat agaggatatt tatcacgtgg agggactgct
     3841 atgagagacg atatcaaaat caatgaccgt gctttggcct tgcaagacca aattatcgaa
     3901 aaactagaga aagtttttga tacagatgtg gaattggatg tttacaatct aggtctgatt
     3961 tatgaaatca atctggatga aacggggctc tgcaagattg tcatgacctt caccgatact
     4021 gcctgtgatt gcgccgaaag cctgcctatt gaaatcgtgg caggtttgaa acaaatcgag
     4081 ggtatcaagg atatcaaggt tgaagttacc tggtcgcctg cctggaaaat cacacgaatc
     4141 agtcgctatg gccgtattgc ccttggacta ccacctcgtt aagcaggtca atcactttta
     4201 aagatgaaaa aagcaagccg aaaatggctt gctttttgat actcaatgaa aatcaaaggg
     4261 caaactagaa aactagccgc aggttgctca aaacactgtt ttgaggttgt agatagaact
     4321 gacgaagtca gtaaccatac ctacggcaag gcgacgttga cgtgatttga agagattttc
     4381 gagtatgagt ttatttttta cctgacttgt ccatattcca gaagtctgtc acggctccgc
     4441 gtgaagcaga tgatacgatg tgggcatatt taccgagtac accacggctg taaagtggtg
     4501 gcaaggttgc ttctgccttg cgtttttcaa gttcttcttc ggatacggcc atggaaattt
     4561 ctttggtatc ttggtcaacc gtaacgatat cgccggtacg gagataggca attggtccac
     4621 catcctgagc ttcaggagcg atatgtccaa caaccagacc ataagtacca ccagagaaac
     4681 gtccgtccgt caagagggcc accttatctc cctgaccttt accaacaatc attgaagaaa
     4741 gtgatagcat ctcaggcata ccaggaccac ctttaggtcc aacaaaacga acaacgacta
     4801 catcgccatc aacgatttca tctgtcagaa cggcctgaat cgcatcttct tctgagtcaa
     4861 agaccttagc tggcccaacg tgacgacgca ctttaacacc tgataccttg gcaactgcac
     4921 cgtcaggagc aaggttcccg ttcaagatga taagcggacc atccgcacgt tttggatttt
     4981 caagtggcat gataactttt tggcctggag tcaagtctgc aaagtcagcc aagttttcag
     5041 ctacagtctt accagtacat gtgatgcgat ctccgtgaag gaaaccattt gccaacaagt
     5101 acttcataac cgcagggaca ccaccgactt cgtagaggtc ttggaagaca tactgaccag
     5161 atggtttcaa gtcggccaag tgaggcacac gttcttgaat cgtattgaag tcctcaagtg
     5221 acaagtcaac atttgcggca tgggcaatgg cgagcaagtg aagagtggcg tttgtagaac
     5281 caccgagagc catcgttaca gtaatagcat cttcaaaggc ttcacgagtc aagatatctg
     5341 atggtttgag accaagttcc aacatcttaa caacagcacg tcctgctgct tcgatatctt
     5401 ctttcttatc agctgattca gctgggtgag aggatgaccc tggcaaactc atccctagaa
     5461 cttcgatagc agttgccatg gtattagcag tatacatacc accacaacca ccagggccag
     5521 ggcaggcatt acattcaaga cgtttcacgt cctcagctgt catgtcaccg tggttccatt
     5581 ttccgatacc ttcaaagaca gaaaccaagt cgatatcttt accatcaaga tttcccggtg
     5641 caatagttcc accataggcg aaaatagctg ggatatccat attagcaata gcaatcatag
     5701 atccaggcat gttcttgtca cagccaccga tagcgacgaa ggcatccacg ttgtgaccac
     5761 tcatagccgc ctcgatggag tccgcgatga tgtcacgaga tgttagagag aaacgcatac
     5821 caggcgttcc catagcgatc ccgtccgcta cggtaatggt tccaaactgt acaggccaag
     5881 cgcctgcaga tttgacacct tctttagcca gtttcccgaa atcatgcaag tgaatgttac
     5941 atggtgtatt ttccgcccaa gtcgaaatca ctcccacaat cgatgtttca aagtccttat
     6001 ctgtcatacc agtcgcacga agcatagcac ggttaggtga tttaaccatg ctgtcataga
     6061 tgctactgcg gtgacgttta tctaattcag tcatcttatc cctcccattt cagtttttac
     6121 tattatagca caatttaaag gcaatgaaca gaataaaatt cttgaatttt cagaaaattc
     6181 tatacgcatg tgaaatattt aaaattaaaa acaacaaagc ggattagtgc actttctgat
     6241 gaccagaata tgctttttaa tccgctttct ttaaataacg tactgtaatt tttacagaaa
     6301 ttctttcaga taagtgtatt taacatctat cttgcattat aaatttctag aaccttctct
     6361 tttatattcg attcactcaa accatactca ttaagaagat aatccatttt ccctacttga
     6421 ccaaatcttt cttgaacacc catccgatga atttttgtta ttccatcatc agagaataat
     6481 tcacataaag cactgccaat tccacctatc tgattgtggt tttctacagt aaatatagtt
     6541 tttccactta acattgtttt tatctgttct ggtatcggtt tgattctaaa taaatctatc
     6601 acacctactg aataacctaa tttagacagt tcatctgcaa ctcgaatact tggagcaacc
     6661 attataccag aagcaacgat tacaagatct tcaccatgcc ttaactcaat gtagccttta
     6721 gaaaaatctt ctccaccttg atacacagga actggagctt ttctaattgt tcgaatatat
     6781 tttagtcctt ttaagtctaa tgtctggttc aatatttcac gaaattggat atcatcagtt
     6841 gcttcaaaaa tgatcgattt aggaattaaa cgtaacaatc caatttcttc aaatggcata
     6901 tgtgtcccac cattcatttc tgcagttact cctgcatctg atccaatcac agtggcatcc
     6961 aattgtgcgt atccaagaga aataaataat tgatcaaata ctcttcgtga agaaaaagga
     7021 ccaaatgtat gaagataagg tctaaacccc tgaatagaca agcctgctgc aaggccgacc
     7081 atttctgctt ccataatccc aacattcaca taacggtctc caaagtcctt ttcaagatta
     7141 tgagtagcca tcgaacttga cagatcggct tctaagacta ctatatcaga atcactttga
     7201 ttagcctcta aaaggaagtc tctatataca tgccgtaatt ctttcgtact tctcatcatt
     7261 ctgtttcctc caattcctga cttaatcttt ctacaactga agttaacatt tgtctctcct
     7321 ctacagtagg gcgaagatga tgattggatt tcatttcttc cagctcttga accccttgac
     7381 ctttaatagt atctaataca atgcacttag gtgatgaatt atttgactgt tttaattgga
     7441 caatcccttc ataaatttct ctaatatctg aacccttgac cctaatggat tcaaatccaa
     7501 atgctgaaaa tttttctacg aaatcacctg gattacaaat atcctttgta aaaccatcta
     7561 attgtttttt gttgtcatca acaaatacaa ttaagttgga taactgttga tgagaagcaa
     7621 actgtatagc ctcccaacat tgtccctcat ttaactcacc atctccaaca atagcataag
     7681 tataaaaggg actctttctt attctctgac catatgcaag tccagttgca acactaattc
     7741 cttgtcctaa agagcccgtt gtcatatcta tgcctggcgt tagatttcta tcaggatgag
     7801 acggtaattt ggttccattt gtatttaaag aatataagaa ttctttgtca aagaaaccat
     7861 tcaaatagag tgtactgtat agagctggtc ctccatgacc ttttgataat atgaaataat
     7921 ctctatctcg tgctgcaaat atttctggag tcattggcat tatttcacca taaagcactg
     7981 ctaaaacttc tacgatagac agacttcctc cgtaatgtcc gaatccaaga tgattcaatg
     8041 ttctaagagt atttaatcgg atgttagtcg caaattttct taactcatct tctctatttt
     8101 cacttaaaat catcccttac tcctcctttg cagatggctt tttaataaag gatactccaa
     8161 acataactgc tagaataaga acaagaccaa tcacaatgcc tgcttgtgaa ccaaattgat
     8221 ttaacattcc taaaataatt cctgatagac caaaatctgc atctgagaaa gttgatcctt
     8281 ggaaaccaag tcctcccaaa actggcatta aaaagactgg aagaaaactg attaaaatac
     8341 cttgtaaaaa tgctccaata gtggctccac gaacaccacc agatgcattc ccaatgacac
     8401 ctgcagtcgc tccacagaag aaatgaggca caacgcctgg taagataaca accgttcctg
     8461 aagcaatcat aattgccata cttactaaac caccaataaa actagagata aatccaatta
     8521 gaactgcatt gggtgcataa gtataaacaa tcggacaatc caaagcaggt tttgaattag
     8581 gtacaagacg ctctgaaata cctttaaagg ctggaacaat ttcacccaaa ataaggcgaa
     8641 cacctgctaa aataacaaat acccctgctg caaattgacc tgctaattgt aaagcataaa
     8701 ctagaccact tgtaccacta ctgatttctt tttctatata ttctgaccct gcaaagatag
     8761 ctacaataat gtaaataact gccatggata aagtaatact aacagtgcta tcacgtaaaa
     8821 aagctaaact ctttggaaat ttaatgtcct ctgttgattt tgatttgtca ccgataaggc
     8881 taccagtaaa accactcaac caatatccca aagaactgaa atgacctaaa gccaccttgt
     8941 catttccagt taattgaacc atatattttt gcacaaatgc tggggaaata ctcataataa
     9001 taccgagtgc taatcctcct agtaagataa gaggcaagct agtaaagcca gcaactgata
     9061 aaatgaccgc aatcatacac gccatatata gagtgtggtg ccctgttaaa aagatatatt
     9121 taaatcgagt aaaacgagcg attaagatat tgaacaccat tcctgcaaac ataatcattg
     9181 cagtagctga gccatatgtt gttaaagcta cagctacaat tgcttcatta ttcggcacaa
     9241 cgccagataa atgaaaagca tgctcaaaca tggtaccaaa tggatttaaa gaattttgta
     9301 caattcctgc accaccagat acaactaaga aaccaacaaa ggtcttaatt ccacctttaa
     9361 taatatcagg taatttcttc ttctgaagaa ctaatcctaa aattgcaatt aaagctacta
     9421 aaatagctgg tgtactaaca atatccaata tgaacttcat catgacgcta gcctcctata
     9481 taagtccttt ttcttcacaa agtttagtaa ttaattctcg tagttcatcc atatcaataa
     9541 tactatttaa gatacgaaca tctccaagat gactagctga atcagctaga tcacgaccaa
     9601 caatccaaat atcagctgca tttggatctg ctccacctaa atcataatgt tcaacttcta
     9661 catccgaaac attcaaatca ctcaatacag attcaatatt catctgtacc ataaaacttg
     9721 aacctaatcc tgaaccacaa gctgtaccaa tttttaacat tatctaatcc tcctgtttaa
     9781 ttatcatttt aatatcatca tagttttttg atgatattaa agtttgaaca tgatttttat
     9841 ctcttaaaat tgttgttaaa tgtgacaaag cctttaaatg actctcatta tcaatggctg
     9901 caatacaaat caacaatctt acctcttgtt ctggattatc caataaataa atcggttctt
     9961 ccaaaactaa cattgacatt cctatttcat tcacaccttc atctggccga gcgtgaggaa
    10021 ttgctactcc cttccctaaa ttaataaaag gtccaaactc ttctactttt tgaatcattg
    10081 cctcagggta gttctccgtt atcttatctt gatccaaaag cggtttagct gctaaacgaa
    10141 tcgcctcctt ccatcctaat ttttgcgaac taacctgata ggtttctttg gtaataagtt
    10201 gttctagcac tggtacaatt tcctttctat catttttttg ataaagataa ttctttaacg
    10261 ccaatcttaa ttccaattct tgtgtaataa ttccatatct tttgacaata ttcaggattt
    10321 gttcaatctc aaaatctcca tactctaaat tcggaaaatc ttttaacact agttctacta
    10381 gttgtattgc ttgctcttca gtcatcataa ccgaaactag ataatttggc ttttctgtct
    10441 ccacctttat ggtagaaaaa accatatcat agtcactact agctttcacc tgtaaatcat
    10501 caatctttga ggttcctata aactcaattt gaggaaataa tgctaataga ttctctttta
    10561 acatcaatga agaactaaca ccattaggac aaatgattgc tgctttatac catttttgag
    10621 gcaaagtatc tgctttcttt aaataacctc cgaaatggat aacaaaatat gctgtttcac
    10681 tatcaggtat gggattgtca atagcgtcca tcaagggcat caaagaatct ttgactaatt
    10741 caaataaatc aggataatgt tctttaacat gcaatacata ttcatttgaa ctaggtaggc
    10801 cgaactttaa tctatagtaa gccggtataa ggtggcggcg aagattttct ctcaatcctt
    10861 ccctttgttt aaaatgtaac aaagaaatat cttccattct acttataata gcctctgtta
    10921 attgattaaa gtaaaccgga gcaacatcta cttcaccttc aaagcaactt gataataaaa
    10981 ctgtgatata gcgataatca tcctcagaaa acaccgtatc tataattccc aaatcaacca
    11041 ctgtatccaa taaaatagtg gttatatctt gaataacagg agatactaat gtctctgaaa
    11101 gacatactct ttcaacatcc ctttgatacc tacacagaat gaatactaaa ccgaaaaggt
    11161 aaacttttaa ttgattaaca ataggtacta gctgtagctt ctcataataa tctttaacta
    11221 cctgatttat caaatcataa gttaatgaat acccccaact ggataaaaca taatccaaac
    11281 cccaaatccc tatggaggat tccagcaact cactaaccat ttgaaaagct aagcggtgct
    11341 tattccactc tgaaccgtgt aaagtataac cttttgctct actgtaccct agctccaaat
    11401 cattatctaa cataatcttt cttaatgatt gaatatcaga taaggttgta ttcttactta
    11461 ctttcaaaaa gtcttggtaa tgattattcg atataaaatc taatcggcaa aaagtgtaaa
    11521 gatagattaa agctaagcga gtcgactttg gtaaaaccaa ttcatccgac ttaataatat
    11581 ctgtcaaaga ctgcttcgta cgatttgata aactatagcg accttgtttt ttatccagca
    11641 ctatcccttt attagctaga taaggcacta aataatctat tccttctttg acttccttta
    11701 taggtaagct caccttaaca gataattcat ataatgatag ctcacaatga tccatcaaag
    11761 tcatcaaaat aactagtgct ctataatcaa acatttctct tcctttctaa ctacaattat
    11821 agtgtaaaaa ttataagaat aaaagagtat ttgagcacaa tataatctca ccttttaaaa
    11881 gaaattattg ggctgaaagt ttttagtgat tattttcaac tttctattga cttttaaaaa
    11941 caaagtgata tcatttagat atcaaataat gggaggtcat tcttatgact gaaaaactaa
    12001 tcaattcaaa accaaatggt gtattcgcat tgattctcat tgagttgaca atcatacttg
    12061 gtatctttat atttataatg ggtgttggtt cggaaaacat ttttggaatt attatcggac
    12121 ctttactaat cgtaattgca ggtctagctc atgctggttt aaaagttgtc aaacctcagg
    12181 aagctctggt tctgacactc tttggtaatt atacaggtac catcaaagaa cctggttttt
    12241 actttgtcaa tcccttcagc gtagcagtca accctgcaaa ccacactcga cttggacaaa
    12301 gtggtgatgt tagcacaaaa tctccttttt taggagctaa atcatcaaat gacaatgatg
    12361 taaatcttga aattggcaag aaacacattt ccctcaaagt catgaccttg agcaattctc
    12421 gtcaaaaaat caatgattgc ttaggaaacc ctgtagaaat cggtatcgcg gtaacttgga
    12481 gagttgtcga taccgctaag gcagtcttca acgttgataa ctacaaagaa tatctttcat
    12541 tgcaatgtga tagcgccctc cgtaatattg tccgcatcta tccttacgat gtgtctccta
    12601 atgtggatac tacgggtgac gggcaagcag atgaaggtag tctccgtggc tctagcgaaa
    12661 ttgttgctaa ccgtattcgt gaagaaatcc aaagtcgtgt tgaggatgct ggcttggaaa
    12721 tccttgaagc acgtatcact tacctagctt atgctccaga aattgctgcc gttatgcttc
    12781 aacgccaaca agcatctgcc attattgatg cacggaagat gattgtagat ggtgctgtag
    12841 gaatggttga aatggcacta gaacggctca atgaagggga attggtagaa cttgacgaag
    12901 aacgaaaagc tgccatggtt tcaaatctcc ttgtcgttct ttgtggcaat catgatgcac
    12961 aaccaattgt caacacagga agtctttact aaagatggct gaagctaaaa aaaagcagat
    13021 cccccttcga ctctcaacaa agttatacgc tgcactcgca tcatgggcag aagacgactt
    13081 tcgttcagtc aatgggcaaa tcgaatatct cctcacagag tgtgtcaagc aacggaaaaa
    13141 agacggaaaa tacgtatcag aaaccattga cgaaccattt gagattgata tttaacatct
    13201 tctcctgtct gacaagtcgg acaggagaat tttttcatct ttttttgtca agtaacttta
    13261 ctttacaaaa aaaatgtgtt atcctagtat ggttgatgaa aatcagtaga ttgaatcgaa
    13321 tataaatacc taaaggagaa atcaaaatgg cagtacctgc acgtcgcact tcaaaagcga
    13381 agaaaaacaa acgtcgtaca cactacaaag taacagctcc atctgtaaac tttgacgaaa
    13441 ctactggaga ttactcacgt tctcaccgtg tatcacttaa aggatactac aaaggacgta
    13501 aaatcgctaa agctgcatca gctgaataat agaagggaga taccatgcgc gtaaatatta
    13561 cacttgaaca caaagaatct ggtgaacgct tgtaccttac ttctaaaaac aaacgtaaca
    13621 ctccagaccg ccttcaattg aagaaatact caccaaaact tcgcaaacac gttgtgttta
    13681 cagaagtgaa gtaagcattc aatacgaatc aatacagaag aaaaacgctt gatcagcgtt
    13741 ttttcttttt gttaatttca ctatattgca agggaaatca ttcgaatgac tacctttttc
    13801 actacctttt ttgaaataag aatacggatt cggacgatta aaagtaagag aaactcaaat
    13861 gtaagttggg gttccttttt tgtatatttt tcaaataacg cctctacact atttgtagca
    13921 aattcaccaa ctacagttgt atcttagtta aaataagtta gaatatgtaa gtgagtacca
    13981 gatataccaa gacatcgtca ccatctaagg tatattcaaa atacaaaagt tgaccaacta
    14041 gatttctgaa tatccttata tatccattct taaaattggt ttaaatagcg tagtctttta
    14101 aactagtttt gagaatccaa aaaatcttcc tacatatgta agaagatttt ttagttcaga
    14161 atgattagat ttagctaatg gatacctatc ctacccactc accgttagca ttgactcgat
    14221 agccatcggg tgtagtcgtg tttactaata aatctcctga attataggta tagtaccatt
    14281 taccagaaac tgtaaaccaa ccagtagcca ttgaacctga ttcatttaag ta
//
LOCUS       DAVKLW010000047        14035 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKLW010000047 DAVKLW010000000
VERSION     DAVKLW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.047"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(13..864)
                     /locus_tag="VW324_001547"
     CDS             complement(13..864)
                     /locus_tag="VW324_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9727155.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            1191..1583
                     /locus_tag="VW324_001548"
     CDS             1191..1583
                     /locus_tag="VW324_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727156.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAVDMARQNWWDLSAAISGSWSPN"
     gene            1605..2396
                     /locus_tag="VW324_001549"
     CDS             1605..2396
                     /locus_tag="VW324_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727157.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            2389..3030
                     /locus_tag="VW324_001550"
     CDS             2389..3030
                     /locus_tag="VW324_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727158.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            3034..3684
                     /locus_tag="VW324_001551"
     CDS             3034..3684
                     /locus_tag="VW324_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9727159.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            3695..3982
                     /locus_tag="VW324_001552"
     CDS             3695..3982
                     /locus_tag="VW324_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727160.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            4128..6641
                     /gene="secA"
                     /locus_tag="VW324_001553"
     CDS             4128..6641
                     /gene="secA"
                     /locus_tag="VW324_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9727161.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            6722..7753
                     /locus_tag="VW324_001554"
     CDS             6722..7753
                     /locus_tag="VW324_001554"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9727162.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            7755..8786
                     /locus_tag="VW324_001555"
     CDS             7755..8786
                     /locus_tag="VW324_001555"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9727163.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            8825..9193
                     /locus_tag="VW324_001556"
     CDS             8825..9193
                     /locus_tag="VW324_001556"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9727164.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            9183..10286
                     /locus_tag="VW324_001557"
     CDS             9183..10286
                     /locus_tag="VW324_001557"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9727165.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            10305..12320
                     /gene="recG"
                     /locus_tag="VW324_001558"
     CDS             10305..12320
                     /gene="recG"
                     /locus_tag="VW324_001558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9727166.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWCMIALHLEKKEHLD"
     gene            12636..13616
                     /locus_tag="VW324_001559"
     CDS             12636..13616
                     /locus_tag="VW324_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9727167.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         4133 a         2552 c         3125 g         4225 t
ORIGIN      
        1 agttattaca aattataggt ttgaaaaatc attaatttct ttttcgagta ttttaactaa
       61 tgcctcttct ttggttagtc gtaataaagt tattccttta tcaatcaaat cggagttatg
      121 ttgtagtatt cccaagcgta cttgagagaa tccaagaata tcgtatattt ttaattcttc
      181 tgctaatacc aaaagttgta gggtgattgt ttcacattct ttgaataaat gatgttgaaa
      241 atatacagtg gagagattgg ctaaaaaaga tgataaaaat gcttttgttt ctcggaagta
      301 tttatatttc tcgatggttt tcataattct atcaataaga aggggaagat tttcagaagg
      361 aaagaaaaag agaatggttc caagcaattt caaatcacta atgtaccatg tatccatagg
      421 ttcaagatag tcccaaattt cttcatacaa atctttcaag acgtggttgt ttttgaatcc
      481 tttagttcgt aactctgtga caatttttgt atgacgatag atattttgaa taggaacatc
      541 gtgatgtgtc ttaagatatt tttgacattt ctcagtgagt tcaaccattt ttctagtatc
      601 ttgaaaagta gacggatttt ggctctctac aataatatct cgccttttac taggatggta
      661 ttcgttgcat atatacttga attctgccaa gctcatatca atttgttcaa gaagaaatac
      721 catgttttca aaactgggaa taactttacc gtgttcaata tttgttagag ttgttcgatg
      781 tatgacttgt ccacatacat cttgttgggt caatcctttt gactttcgaa tttctttaaa
      841 aatttgtcca taatcccatc tcatattgtt cctcctaggt aaatgtgaat attttctaca
      901 tttataaaaa acgtagaaaa aggtggtatt attatatcaa aaaggaggga aaaagtcatg
      961 ggctttaaaa aatatttgaa gaatttaccg aaaaactctg gatttttgat ttggagttgg
     1021 attcaactta tctggtttga aacatggttt tggggataaa gaaattagag tctatacata
     1081 ataagtaaag gaggaatttg tatgatatac catcgtttag aataagtcta tatttaaaat
     1141 agaagttata aggaatcttc ttgaataaaa aatgaaagga aacagaaaaa atgaaaatta
     1201 agaaattatt gaaaatggtt attcctgttt taatgataag tgctgttgga actactttcg
     1261 tagaagcaaa tcagataggt gcttttagta attttgttat tactaccagt tataagagaa
     1321 caggttattt gactaaagaa aatgaaggtg cggaatatat catgaactta aatccttgta
     1381 gaaatttaca tcccatgact gttaagcatc gtatagtaaa ttctaatgga gaagctcgta
     1441 gtggagaatc gttaacaacc tgtggtactc gttcaactca tggtaattgg gcaacggttg
     1501 gctatgttta tgcagtagat atggcaagac aaaattggtg ggatttatca gctgccattt
     1561 caggtagttg gtcaccaaat taattcgtaa agtaggtata atttatgaag attcctctct
     1621 taacttttgc aaggcataaa tttgtttatg tcttgcttac tttgcttttt cttgctttgg
     1681 tttatcgtga tgttttgatg acttatttct tttttgatat tcatgcgccc gatctagcta
     1741 aattcgatgg acaagcaatt aaaaatgact tattaaaatc agcattagat tttcgtattc
     1801 tccagttcaa tctaggtttt tatcaatcat ttattattcc aatcatcatt gttttgctag
     1861 gttttcaata tattgagctg aaaaataaag ttttacgatt gagtattgga agagaagtga
     1921 gctatcaagg gttaaaaaga aagttgactt tgcaagttgc aagtatccct tgtttgatat
     1981 atttagtgac tgtgctgata attgcaatta taacctattt ctttgggact ttttctcctc
     2041 ttgaatggaa ttctctattt tctgatggga gtggtttaca aagactccta gatggagaga
     2101 taaaaagcta tttgttcttt acttgtgtcc tattaatcgg tattttcatc aatgcaatct
     2161 attttttaca aatagttgat tatgtgggga atgtgactcg ttcggcaatc acctatttga
     2221 tgtttctttg gcttggttct atgctgctct atagtgcctt gccttactat atggttccta
     2281 tgacgagttt gatgcaagct agctatgggg atgtaagttt gatgaaactc tttactcctt
     2341 atatccttta tgttgtccct tatatggtac ttaaaaaata tgaagataat gtttaagaat
     2401 tttaacaata tttggctaaa gagaaagatc gttttactac ttcgtatagt tctgatgatg
     2461 attttgataa actatctatt gtcaacagcg gttcaaaagc aggatgctgt tatctttttc
     2521 aagagagaat tgatttcaat tttttcctat aatgactatt ctgaagcgaa tttagaaatc
     2581 cccaaactct tgttaaacct ttcgcttttc atggtaggat ggctctctgt cattttactt
     2641 gaaagtgatt tggcagacca ttaccatcac ttgattcgct atcaatcaag ctcctttttc
     2701 gattatacaa ggaaacgatt ggttgtcatt tctaaatttt ttactcaaga tttgtttgtc
     2761 tggtttcttg gtttacttcc gctaggaatt catttcaaaa cagtcgcact tttcttttta
     2821 cttgctcagt taatgatgtt gtacttacta ctgtcttatc tgatagcact gattagtgcg
     2881 ggcgctggtt tttctttttt tctctatttt ttggcatttg tgggacaaga atggatgatg
     2941 gatcatattg taacagtgta tttagtactc ttaagcttat tagttatgtt gattcttagt
     3001 cgcttggaag agaaatttaa gaaaggataa acgatgagac ttgaaattat aaatggacag
     3061 aaaatttatg ggaaaagacc tattttaaat cagttgaatt tggtgtttca atcaggaaaa
     3121 atttatggac ttaaaggtga taatggatct ggcaagacgg ttcttttaaa gatacttgct
     3181 ggttatatta agcttgacaa aggaaaagtt cttcaagatg gtaaagttta cggggtaaaa
     3241 aatcattata ttcaggatgc aggaatttta attgaaaaag tcgagttttt atctcattta
     3301 tccctgagag aaaatttgga actgttaagg tatttttcat ctaaagttac ggaaaaaaga
     3361 attgcctatt ggattcaata ctatgattta caggaatttg aagacgttga ataccgtcat
     3421 ttatccttgg gaacaaagca aaaaatggcc ttgattcaag cctttatttc ctctccttct
     3481 atactctttc tcgatgaacc tatgaatgct ttggatgaga aaagtgtgag gttaaccaaa
     3541 caggtcattt tatcttacct gaaaaaagaa aatggtctgg ttatcctgac gtcgcacata
     3601 tcggaagata tttcagacct ttgtacagat gtattagttg tcgaaaatgg acatatacaa
     3661 tatgtaaagg atatacaatc ctaggagatg gcttatggca catctaaaat catttattac
     3721 acgctattcc aaggtttata ttggtttagt tctgctgatc tggctgtctt tcttctttat
     3781 cccttgggat agagcgattc tgggggtaag gattgacatc ttcatcatac agaaagtctt
     3841 gctagttttt ggaattctgt ccattttgat ggccttgctg tccaagaaag tcagtctctt
     3901 tgtttttgga ctgatttgct gtctttctct ttggattaac ttatttatca catttgccat
     3961 tttgccaatt tttggcaatt aaacagtcat aaaagtcgga gaggttagct tgaaaactaa
     4021 cctctttttc cttttcaaaa tggggattct tccttgaaaa taatcagtaa ttgtgctaaa
     4081 attaaaggaa cattctaaaa tattcggaat ttaaagtaag gaaaaacatg gctaatattt
     4141 taaaaacaat tatcgaaaat gataaaggag aaatccgtcg tctggaaaag atggctgaca
     4201 aggttttcaa atacgaagac caaatggctg ctttgactga cgaccaacta aaagcaaaaa
     4261 cagttgaatt taaggaacgt tatcaaaatg gagaatcact ggattcattg ctttacgaag
     4321 catttgcggt tgtccgtgaa ggtgccaaac gtgtcctagg tctcttccct tataaggttc
     4381 aggtcatggg ggggattgtt cttcaccatg gtgacgtgcc agagatgcgt acaggggaag
     4441 ggaaaacctt gactgcgacc atgccggtat acctcaatgc cctttcaggt aaaggggttc
     4501 acgtagttac ggttaatgaa tacctgtcag aacgtgacgc gactgagatg ggtgaattgt
     4561 actcttggct tggtttgtca gtagggatta acttggctac caaatctcca atggagaaaa
     4621 aagaagccta tgagtgtgat attacttact caactaactc agaaatcgga tttgactacc
     4681 ttcgtgacaa catggtcgtt cgcgctgaaa acatggtaca acgtccgctt aactatgcct
     4741 tggtcgatga ggttgactct atcttgattg acgaggctcg tacacctttg attgtatcag
     4801 gtgccaatgc ggttgaaacc agtcagttgt atcacatggc agaccactat gtaaaatctt
     4861 tgaacaaaga tgactacatc atcgatgtgc agtctaagac tattggtttg tctgattcag
     4921 ggattgacag ggctgaaagc tacttcaaac ttgaaaacct ctatgacatc gaaaacgtgg
     4981 ctttgaccca ctttatcgat aacgcccttc gtgccaacta catcatgctt ctcgatattg
     5041 actatgtggt gagcgaagag caagaaatct tgattgtcga ccaatttaca ggtcgtacca
     5101 tggaaggtcg tcgttattct gatggattgc accaagctat tgaagccaaa gaaggtgtgc
     5161 caatccagga tgaaaccaag acatctgcct caatcacgta ccaaaacctt ttccgtatgt
     5221 acaaaaaatt gtctggtatg acgggtacag gtaagactga ggaagaagaa tttcgtgaaa
     5281 tctacaacat tcgtgttatt ccaatcccaa caaaccgtcc tgttcaacgt attgaccact
     5341 cagaccttct ttatgcaagt atcgaatcta agtttaaagc ggttgtcgaa gacgttaagg
     5401 ctcgttacca aaagggtcaa cctgtcttgg ttggtacagt agcggttgaa actagtgact
     5461 acatttctaa gaaattggtt gcagctggtg ttcctcacga agtcttgaat gccaaaaacc
     5521 actatagaga agcccaaatc atcatgaatg ctggtcaacg tggtgccgtt accatcgcaa
     5581 ccaacatggc gggtcgtggt accgacatca agcttggtga aggtgttcgt gaacttggag
     5641 gactttgtgt tattggtaca gaacgtcatg aaagtcgtcg tatcgataac cagcttcgtg
     5701 gacgttcagg tcgtcaagga gatccaggtg agtcacaatt ctacctatct cttgaagatg
     5761 atttgatgaa acgttttggt tctgaacgtt tgaagggaat ctttgaacgc ttgaacatgt
     5821 ctgaagaggc cattgagtct cgcatgttga cgcgtcaggt tgaagcagct cagaaacgtg
     5881 tcgaaggaaa taactacgat acccgtaaac aagtccttca atacgacgat gtcatgcgtg
     5941 aacaacgtga gattatctat actcaacgtt atgatgtcat tactgcagac cgtgacttgg
     6001 cacctgaaat tcaggcaatg atcaaacgca cgattggtcg tgtcgttgac ggtcatgcgc
     6061 gtgctaaaca agatgagaaa ctagaggcaa ttttgaactt cgctaagtac aacttgcttc
     6121 ctgaagattc tattacgatg gaagacttgt caggcttgtc tgataaggcc atcaaggaag
     6181 agcttttcca acgtgccttg aaggtttacg atagtcaggt ttcaaaacta cgcgatgaag
     6241 aagcagttaa agaattccaa aaagttttga ttctacgagt ggtggataac aagtggacag
     6301 atcatatcga tgcccttgat caattgcgta acgcggttgg acttcgtggc tatgctcaga
     6361 acaaccctgt tgttgagtat caggcagaag gtttccgtat gtttaatgat atgattggtt
     6421 cgattgagtt tgatgtgaca cgcttgatga tgaaagcaca aattcatgaa caagaaagac
     6481 cacaggcaga acgtcatatc agtacaacag cgactcgcaa tatcgctgct caccaagcaa
     6541 gtatgccaga agatttggat ttgagccaga ttggacgcaa tgaactttgc ccatgtggtt
     6601 ctggtaagaa atttaaaaac tgtcacggta aaagacaata aaatgagata gtttagaggc
     6661 ggatatcttg tgaaaagtaa atttttactg ggtatccgtt tgctttataa ggagatgagt
     6721 tatggtattt acagcaaaaa gctctaaaat aaatatagaa gaagttcgtg ccttgtcaaa
     6781 attagaaggt caggctttgg agaggaaatc acagcgagat caagagctag aagccattat
     6841 acgtggagaa gaccagcgaa ttctcttggt aatcgggcca tgctcatctg acaacgaaga
     6901 agctgtcctt gaatacgcta agcgtttggc agtcctacaa gaagaagtgg cagatcgtat
     6961 ctttatggtt atgcgtgttt atactgccaa accccgtacc aacggagatg gctataaggg
     7021 cttgattcac cagcctaacg cgacagaagc gcctagtctt atcaatggaa tcaaagccgt
     7081 tcgccatctt cactatcgtg tcatcacaga aacagggatg acaactgctg atgaaatgct
     7141 ttatcctgaa aaccttccgc ttgtagatga tttgatttct tacatggcag ttggtgcccg
     7201 ttcagttgaa gaccagcaac accgctttgt ggcaagtggg gcaggatttt ctactggttt
     7261 taaaaatcca acctctggaa atctcaatgt catgtttaat gggatttatg ctgctcaaaa
     7321 caaacaaagt ttccttttct taggaaaaga agtagaaaca actgggaacc cgctttcaca
     7381 cgctattctt cgtggtgctc ttaatgagta tggaaaaaat attcccaact actattatga
     7441 caatttaatt gataccattg cccagtatga gaaaatgggc ttggaaaatc cttttatcat
     7501 cattgatacc aatcatgaca attctggtaa gcagtatatt gaacagatcc gaattgtccg
     7561 ccagaccttg attaaccgtg cttggaatga aaaaattaag cagttcgttc gtggttttat
     7621 gattgagtct tatctggaag atggtcgaca aaatgagcca gaagtatttg gtaagtctat
     7681 cacagaccct tgcctgggtt gggataacac agaagctctt gtcagagaaa tttacaaaac
     7741 gttaggagaa taagatggca tttattgaaa aaggtcaaga aatcgatatg gaagtcatca
     7801 aggctgaaac ccaattgtct gcagaagcct tgagactcaa ggaaagccgt gacagggaat
     7861 tggcagatat tatttcaggg gaagatgacc gtattctctt ggtgattggt ccttgctctt
     7921 ctgataatga agaggcggtc ttggaatatg ctcgccgttt atccgccttg caaaagaagg
     7981 tagcggacaa gattttcatg gtcatgcgcg tgtatactgc taagcctcgt accaatggag
     8041 acggctataa aggattggtt caccagccag atacttctaa ggctccaagc ctgattaacg
     8101 gcttgcaggc tgtgcgccag ttgcactacc gcgtgattac agagactggt ttgacaacgg
     8161 cagatgagat gctttatccg tcaaatctga tcttggtgga tgacttggtc agctaccatg
     8221 ccgttggagc tcgttctgtg gaagaccaag agcaccgctt tgtggcttct gggattgatg
     8281 caccagtagg gatgaaaaat ccaacctcag gaaatttggg tgttatgttt aacgccatct
     8341 atgctgctca aaacaagcaa accttccttt atcatgggca ggaagttgag acatcaggta
     8401 atcctttggc ccatgttatc ctccgtggag cagtcaacga gtatggcaat tatatgccga
     8461 attactacta tgaaaaccta ctccaagcca ttgaacgata tgaaaccatg ggacttgaaa
     8521 atccttttat cctcattgac accaaccatg ataactcagg caagcaatat atggagcaga
     8581 ttcgaattgt tcgccagacc ttgcagaatc gtgattggaa tgagaaaatt aaaaagacgg
     8641 ttcgaggatt tatgattgaa tcttacctag cagatggtcg tcaaaaccaa ccagagatct
     8701 ttggttgctc tattactgac ccttgcctag gttgggaaaa tacagaggcc ttggtagaag
     8761 agatttatgt taccttgaca aaataagtga aaaggatgga gttggggaat ctcaactcct
     8821 tttgatgaga atgatagttg gacacggaat tgacatcgaa gaattggctt cgatagaaag
     8881 cgcagttaca cgacatgaag gatttgctaa gcgtgtactg accgctcagg aaatggagcg
     8941 cttcaccagt ctcaaaggac gcaggcaaat agaatattta gctggtcgct ggtcggctaa
     9001 ggaggccttt tccaaggcta tgggaacggg cattagcaag ctcggttttc aggatttgga
     9061 agtcttgaac aatgaacgtg gggcgcctta ttttagtcag gcaccatttt caggaaagat
     9121 ttggctgtct atcagccaca ccgatcagtt tgtgacagcc agtgtcattt tggaggaaaa
     9181 tcatgaaagc tagtccacat agaccaacca aggctctgat tcatctggga gctattcgac
     9241 aaaatattca gcaaatgggg gctcatatcc ctcaaggaac gctcaagttg gctgtggtca
     9301 aggccaatgc ttatggtcat ggagctgttg ccgttgccaa ggcaattcaa gatgatgttg
     9361 atggcttttg cgtttccaat atcgatgaag ccattgaact cagacaagct ggactcagca
     9421 agccaatcct cattttagga gtttctgaaa tcgaagctgt tgctctagct aaagaatatg
     9481 acttcacctt gacagtggct ggactggagt ggattcaagc actcttagat aaggaagtgg
     9541 acctaactgg attgacagtc cacctcaaga ttgattcagg gatgggacgg attggtttta
     9601 gagaggcaag tgaggttgag caggctcaag atttgctcca acaacacggt gtttgtgttg
     9661 aaggaatctt tacccacttt gctactgctg atgaggaatc agatgactat tttaatgccc
     9721 agttagaacg gtttaaaact attttagcta gtatgaagga agttccagag ctggttcatg
     9781 ctagcaattc tgcaacgact ctttggcatg tagagactat tttcaatgcg gttcgtatgg
     9841 gagatgccat gtatggcctc aatccaagtg gagcggtctt ggatttgcct tatgatttga
     9901 taccggcctt gaccttggag tctgctctgg ttcatgtcaa gacagttcca gctggagctt
     9961 gcatgggcta tggagcaact tatcaagcgg atagcgagca agtcatcgcg accgtgccaa
    10021 tcgggtatgc agatggatgg acaagagaca tgcaaaattt ctctgtcttg gtagatggcc
    10081 aagcttgccc aattgtcggc agggtttcga tggaccaaat cactattcga ttgcctaagc
    10141 cttacccact cggaaccaag gtaaccttga ttggctccaa tggggataag gaaatcactg
    10201 caactcaggt agcgacctac cgcgtaacca ttaactatga ggtggtttgc ctcctcagcg
    10261 accgtattcc gagagaatat tattagaaaa gaaaggagtg gagcatgaat ctacatcaac
    10321 ccttgcatgt cttgcctggt gtgggaccaa agtcagcaga aaaatatgcc aaactaggaa
    10381 ttgaaaactt gcaagatctc ttgctctact ttcctttccg ttatgaagac ttcaaaacca
    10441 agcaggtgct ggagctggaa gacggtgaga aggcagttct ttctggtcag gtagtgactc
    10501 ctgctagtgt ccagtattat ggtttcaagc gcaatcgcct gcgttttagt ctcaagcagg
    10561 gagaggtcgt ttttgcggtg aatttcttta accagcccta tctggctgat aaaatagagt
    10621 tgggagcaac ccttgctgtc tttggaaaat gggaccgcgc taaggctagt ctgactggga
    10681 tgaaggttct ggctcaggta gaagatgacc tccagcctgt ctatcgtctg gctcagggaa
    10741 tcagtcaggc cagtctggtt aaggtcatca agacggcttt tgatcaggga ctggacctct
    10801 tgatagaaga aaatctgccc cagtctttac tagacaaata caaactcatg tcccgttgtc
    10861 aggcagtccg agctatgcat tttccaaagg atttggcaga atacaagcag gctcttcgcc
    10921 gtatcaagtt tgaggaactc ttttatttcc aaatgcagtt gcagacgctc aagtctgaaa
    10981 atagagttca gggaagtggt ctggttctga attggtctca ggaaaaagtg acagcagtta
    11041 aagtaagtct tccttttgcc ctgacccaag ctcaggaaaa gagtttgcag gaaattttaa
    11101 ctgatatgaa gtccgaccac cacatgaatc gtctcctaca aggggatgtg gggagtggaa
    11161 aaacggtagt cgctggcttg gccatgtttg cggcagtgac agcaggttat caggctgccc
    11221 taatggtacc aacagaaatc ctcgcagagc aacactttga gagtttacag aacctttttc
    11281 ccaatttgaa actggctctc ttgacaggtt ccttgaaagc tgcagaaaag agagaagtct
    11341 tggagaccat tgccaagggt gaggctgatt tgattatagg aactcacgct ctgatacaag
    11401 atggggtgga gtatgctcgt cttggtttga ttattatcga tgagcagcac cgttttggtg
    11461 tagggcaaag gcgtatttta cgggaaaaag gtgacaatcc agatgtcctc atgatgacgg
    11521 cgactcccat tccacggacg cttgccatca cagcctttgg agatatggat gtttccatta
    11581 tcgaccagat gccagcaggt cggaagccta ttgtgacgcg ctggatcaaa catgagcaac
    11641 tacctcaggt cttgacttgg ttagaggggg aaattcaaaa aggttcccaa gcctatgtca
    11701 tctctccttt gattgaagaa tcagaagctc tagatttgaa aaatgccatt gccttatcag
    11761 aggagttgac gactcatttt gcaggcaagg cagaggtggc tcttctacat ggtaggatga
    11821 agagtgacga aaaagaccag atcatgcagg atttcaagga gagaaagacg gatattctgg
    11881 tttcgacgac ggttattgag gttggggtca acgttcccaa tgcgactgtc atgattatca
    11941 tggatgccga tcgcttcggt ctcagtcaac ttcaccagct tagaggtcgt gtcggtcggg
    12001 gagacaagca gtcctacgct gttctcgttg ccaatcccaa gacggattct gggaaagacc
    12061 gcatgcgcat catgacagaa acgaccaatg gatttgtcct tgcggaggaa gatttgaaaa
    12121 tgcgtggttc tggtgagatt tttggaacca gacagtcagg acttccagag ttccaagtgg
    12181 ctgatattat cgaagatttt ccgattttag aagaagcaag aaaggttgct agctacatta
    12241 gttctataga agcttggcaa gaagatccag agtggtgcat gattgccctt catctggaaa
    12301 agaaagaaca tctggattaa gctttctcta aggaaaactt aagctagttt ttaggtttgg
    12361 ctcttatact aaagtcatca aaaagaaacg aggactctca tatgacagta acgattaaag
    12421 taaattacca aaccactttc caaaagaagg aagcaaaaaa ctagtataaa cagaagagag
    12481 agcgaaatgc tctttttcgt ttctaaaact actttcagcc catcatccta aaagtaaaga
    12541 atctaaattc actttctatt tatccttctt tcttgcattg attacataga tatgctacag
    12601 ttgtggtaac gattacaaaa taaaaggagc atgctatgaa aaatccagct ttgctagaag
    12661 aaattaagac ctatagagga agggatgagg ttccggaaga ctttgatgct ttctgggatg
    12721 gggaagtgaa aaatgtttcc acgcttccat cctaccactt ggaggaaaga gatttccaca
    12781 ttcctcaagt caagtgctat gagttaacat ttgaaggaag caaggaagga aaggtctatg
    12841 cacgcattgt tcttccaaag agtgaggaga aggttccact aatcttccat tttcatggtt
    12901 atatgggacg tggctgggac tgggccgaca tgctgggctt caccgtagct ggttacggtg
    12961 ttgtttccat ggatgtgcgg ggccagtcag gttactcaca agacggcttg cgttctcctt
    13021 taggaaatac cgtgaagggg catattatcc gtggtgctat ggaaggtcgg gaccacctct
    13081 tttataagga tgtttatctg gatatttacc agttggtcga aattgttgct agtctgtctc
    13141 aggttgatga gaagcgtctt tctagctatg gtgcctcaca aggaggggct ctagctctag
    13201 ttgcagcagc gctcaatcct cgaattcaga aaacagttgc catttatccc ttcttgtcag
    13261 acttcagacg ggtgattgag attggtaata ctagcgaggc ttacgacgaa cttttccgtt
    13321 atttcaagtt tcacgacccc ttccatgaaa cagaggagga aatcatggcg acccttgcct
    13381 atatcgatgt caaaaatctt gcccatcgta tccaaggtga ggttaagatg attacgggct
    13441 tggacgacga tgtttgctat cccattaccc agtttgcgat ttataatcgt ctgacctgcg
    13501 ataaaaccta tcgcatcatg cctgagtatg ctcacgaagc catgaatgta tttgtcaatg
    13561 accaagtcta caactggctc tgtggaagtg agattccttt taaatatcta aaataaggag
    13621 tcgactctaa gcacaaaatc ttaaaaatta caaacacgca tagtatcagg ggattaaaaa
    13681 aacttgatac tatgcgtttt atcatggaaa tatagtaaaa tgaaacaaga ataggacaaa
    13741 acgatcagga cagtcaaatc gatttctaac aatgttttag aagcaaatgt gtactattct
    13801 agtttcaatc tattatattt atagaatttt ttgttgctag atttgtcaaa ttgcttaaaa
    13861 taattttttt cagaaagcaa aagccgatac ctatcgagta gggtagttct tgctatcgtc
    13921 aggcttgtct gtaggtgtta atacttttca aaaatctctt taaaccacgt cagcttcgcc
    13981 tcgccgtata tatgttactg acttcgtcag ttctatctgc cacctcaaaa cggtg
//
LOCUS       DAVKLW010000048        13815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKLW010000048 DAVKLW010000000
VERSION     DAVKLW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.048"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(192..1823)
                     /locus_tag="VW324_001560"
     CDS             complement(192..1823)
                     /locus_tag="VW324_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9727168.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLHQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2112..7052
                     /locus_tag="VW324_001561"
                     /pseudo
     CDS             2112..7052
                     /locus_tag="VW324_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            7142..8338
                     /locus_tag="VW324_001562"
     CDS             7142..8338
                     /locus_tag="VW324_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9727169.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8474..9001
                     /locus_tag="VW324_001563"
     CDS             8474..9001
                     /locus_tag="VW324_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9727170.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9078..9434
                     /gene="glnR"
                     /locus_tag="VW324_001564"
     CDS             9078..9434
                     /gene="glnR"
                     /locus_tag="VW324_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9727171.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9471..10817
                     /gene="glnA"
                     /locus_tag="VW324_001565"
     CDS             9471..10817
                     /gene="glnA"
                     /locus_tag="VW324_001565"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9727172.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLELY"
     gene            complement(10913..11032)
                     /locus_tag="VW324_001566"
     CDS             complement(10913..11032)
                     /locus_tag="VW324_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9727173.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11290..11427
                     /locus_tag="VW324_001567"
     CDS             11290..11427
                     /locus_tag="VW324_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727174.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEDLNIRLEVK
                     N"
     gene            11577..12896
                     /locus_tag="VW324_001568"
     CDS             11577..12896
                     /locus_tag="VW324_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9727175.1"
                     /translation="MPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDT
                     EKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAIS
                     NYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRII
                     EAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLL
                     EKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYI
                     QRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNS
                     TGLGTLGRLAIYHENKNPYVWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEE
                     KASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            12953..13750
                     /gene="psrA"
                     /locus_tag="VW324_001569"
                     /pseudo
     CDS             12953..13750
                     /gene="psrA"
                     /locus_tag="VW324_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
BASE COUNT         4512 a         2435 c         2908 g         3960 t
ORIGIN      
        1 atgaaataat aacaggataa atcgatcagg acagtcaaat cgatttctaa caatgtttta
       61 gaaatagagg tgtactattc tagtttcaat atactatata tctgcagaat actctttcag
      121 tttcattcag cctagtcttg cttaggaaaa agtaaaaagc catccaatta tgatggatag
      181 cttcttggtt cttagatttg ttctgcaaaa accttttcag caaggttcat agcgtggtct
      241 gatacacgag tgtagtgtga gatgatgtcg ataaagttga ccccagcttg tgttgaacat
      301 tcgcctttgt tgaggcgttt gatgtgggtt tttctgagaa cacgttctat cttattgatt
      361 gcttcatgac gttctacaag actgcgtgct ttttcaatat cattgttttc cacactatcc
      421 agagcatctt tgataaagtc actagtttgg cggtaaactt cctctaattc tttcaaggcg
      481 gcatcagaaa attcaacatt tttccgttga agatagtcag tcagattgag tagagcctcc
      541 gtgtggtctc caatccgttc caaatcacgg gaggaatcaa ggatattggt aagcacttca
      601 ctttcttttt ggctgagagc ttcgcttgaa agggcaatga gataacgtgt taattgctca
      661 tcgatggtgt taattgcttc ttcggtttta tgcccttttt cagcaacttt ttcatccaag
      721 tcaatgatgt acttatagga aaggtcaaag gctttagcag cgtagtttcc taagtgcaag
      781 agctctttct tagcatttcc tagagcgata gatggggcct gtttgatgaa atgttcatca
      841 agatataagg gttcgtattt gacaacctcg tcctctccag gaataatctt ggttacaaag
      901 taagccagag ctccgataaa tggaaattgg acaatggtgt tggtaatatt aaaggttccg
      961 tgagcaaagg cgatggtcat ttccggtgct agatttagcg tagcttcaaa ccaatgaatc
     1021 aggacagtaa aaggaactag aaaaataacg cagacgactg ttccgataac gttgaaggca
     1081 acatgagctc ctgctacccg tttagctgca atattagccc ctaaagaggc aatgatggct
     1141 gtaatggttg tcccgatatt gtcaccaaat agaaccggca aagctccctg taggtcaatt
     1201 agattgccgg cgtagaggtt ttgtaaaatc ccaatggtag ccgaagaagc ttgaattagc
     1261 aaggtcaagc cagtaccgac aaagacaccc aaaacaggat ttttacttag ctcaatcata
     1321 tagtccttaa agacctgtaa atccttgagt ggagccattg cgccgctcat gagattgagg
     1381 gcaaaaaaga taccaccgac accaaagagg atgcgtccga tattattgac tgtccgattt
     1441 tttgtaaaaa agagacagac ggcaccgata aagagcatag gtagggcata gttacctaat
     1501 ttaaaaccga tgagaaagga tgtgacagtt gtcccaatat tagcacccat gacaatcccg
     1561 atagcctgat gtaaggttaa gagaccggca ctgaccaggc cgactgtgat aactgttaca
     1621 ccagaactag actgaattag agcagtcatc ccaataccaa ccagaactcc aaagaaagga
     1681 ttactagtat atttgtcaat gtaaaaacga aggcgatctc cagcagcttg ttgtaaaccg
     1741 tctcccatgg tcttgatact atataagaat agccccagac cacctaaaaa gtgaaataaa
     1801 atttcctgcc aattaatgga catttctttt tcctccgaaa aataatcgcg gaatatctcc
     1861 tattctattt taaaggataa aagtaaatct aacaagtgtt aatgtcaagt ttgtaaaaga
     1921 aaattataat tgataagtgt attattttta agagttttta accttatttt agatttgtga
     1981 aaacagctat tgttttttgt ctaaaaatgt tttaggataa aaatgtgatt atcatgattc
     2041 tagagttaaa aaactacaga atataaagcg ctttcttgaa aacaacaaaa tcaatctttt
     2101 aggaggagaa aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt
     2161 tatcagtagg agcctgctcg ctgatgattg gtgctgtttt atttgctggt ccagccttgg
     2221 ctgaagaaac tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga
     2281 gtgagcattc gaccaatgaa gctgataagc agaatgaagg ggaacatact agagaaaaca
     2341 agctagaaaa ggcagaagga gtagcgacag catctgaaac tgcagaagca gctagcgcag
     2401 ctaaaccaga ggaaaaagca agtgaggtgg ttgcagaaac accatctgca gaagcaaaac
     2461 ctaagtctga caaggaaaca gaagcaaagc ccgaagcaac taaccaaggg gatgagtcta
     2521 agccagcagc cgaagctaat aagactgaaa aagaagtcca gccagatgtc cctaaaaata
     2581 cagaaaaaac attaaaacca aaggaaatca aatttaattc ttgggaagaa ttgttaaaat
     2641 gggaaccagg tgctcgtgaa gatgatgcta ttaaccgcgg atctgttgtc ctcgcttcac
     2701 gtcggacagg tcatttagtc aatgaaaaag ctagcaagga agcaaaagtt caagccttat
     2761 caaacaccaa ttctaaagca aaagaccatg cttctgttgg tggagaagag ttcaaggcct
     2821 atgcttttga ctattggcaa tatctagatt caatggtctt ctgggaaggt ctcgtaccaa
     2881 ctcctgacgt tattgatgca ggtcaccgta acggggttcc tgtatacggt acactcttct
     2941 tcaactggtc taatagtatt gcagatcaag aaagatttgc tgaagctttg aagcaagacg
     3001 cagatggtag cttcccaatt gcccgtaaat tggtagacat ggccaagtat tatggctatg
     3061 atggctattt catcaaccaa gaaacaactg gagatttggt taaacctctt ggagaaaaga
     3121 tgcgccagtt tatgctctat agcaaggaat atgctgctaa ggtaaaccat ccaatcaagt
     3181 attcttggta tgatgccatg acctataact atggacgtta ccatcaagat ggtttgggag
     3241 aatacaacta ccaattcatg caaccagaag gagataaggt tccggcagat aacttctttg
     3301 ctaactttaa ctgggataag gctaaaaatg attacactat tgcaactgcc aactggattg
     3361 gtcgtaatcc ttatgatgta tttgcaggtt tggaattgca acagggtggt tcctacaaga
     3421 caaaggttaa gtggaatgac attttagacg aaaatgggaa attgcgcctt tctcttggtt
     3481 tatttgcccc agataccatt acaagtttag gaaaaactgg tgaagattat cataaaaatg
     3541 aagatatctt ctttacaggt tatcaaggag accccactgg ccaaaaacca ggtgacaaag
     3601 attggtatgg tattgctaac ctagttgcgg accgtacgcc agcggtaggt aatactttta
     3661 ctacttcttt taatacaggt catggtaaaa aatggttcgt agatggtaag gtttctaagg
     3721 attctgagtg gaattatcgt tcagtatcag gtgttcttcc aacatggcgc tggtggcaga
     3781 cttcaacagg ggaaaaactt cgtgcagaat atgattttac agatgcctat aatggcggaa
     3841 attcccttaa attctctggt gatgtagctg gtaagacaga tcaggatgtg agactttatt
     3901 ctactaagtt agaagtaact gagaagacca aacttcgtgt tgcccacaag ggaggaaaag
     3961 gttctaaagt ttatatggca ttctctacaa ctccagacta caaattcgat gatgcagatg
     4021 catggaaaga gctaaccctt tctgacaact ggacaaatga agaatttgat ctcagctaac
     4081 tagcaggtaa aaccatctat gcagtcaaac tatttttcga gcatgaaggt gctgtaaaag
     4141 attatcagtt caacctagga caattaacta tctcggacaa tcaccaagag ccacaatcgc
     4201 cgacaagctt ttctgtagtg aaacaatctc ttaaaaatgc ccaagaagcg gaagcagttg
     4261 tgcaatttaa aggcaacaag gatgcagatt tctatgaagt ttatgaaaaa gatggagaca
     4321 gctggaaatt actaactggc tcatcttcta caactattta tctaccaaaa gttagccgct
     4381 cagcaagtgc tcagggtaca actcaagaac tgaaggttgt agcagtcggt aaaaatggag
     4441 ttcgttcaga agctgcaacc acaacctttg attggggtat gactgtaaaa gataccagcc
     4501 taccaaaacc actagctgaa aatatcgttc caggtgcaac agttattgat agtactttcc
     4561 ctaagactga aggtggagaa ggtattgaag gtatgttgaa cggtaccatt actagcttgt
     4621 cagataaatg gtcttcagct cagttgagtg gtagtgtgga tattcgtttg accaagccac
     4681 gtaccgttgt tagatgggtc atggatcatg caggagctgg tggtgagtct gttaacgatg
     4741 gcttgatgaa cactaaagac tttgaccttt attataaaga tgcagatggt gagtggaagc
     4801 tagctaagga agtccgtggt aacaaagcac acgtgacaga tatcactctt gataaaccaa
     4861 tcactgctca ggactggcgc ttgaatgttg tcacttctga caatggaact ccatggaagg
     4921 ctattcgtat ctataactgg aaaatgtatg aaaagcttga tactgagagt gtcaatattc
     4981 cgatggccaa ggctgcagcc cgttctctag gcaataacaa ggtacaagtt ggctttgcag
     5041 atgtaccggc tggagcaact attaccgttt atgataatcc aaattctcaa actccgctcg
     5101 caaccttgaa gagcgaagtt ggaggagacc tagcaagtgc accattggat ttgacaaatc
     5161 aatctggtct tctttattat cgtacccagt tgccaggcaa ggaaattagt aatgtcctag
     5221 cagtttccgt tccaaaagat gacagaagaa tcaagtcagt cagcctagaa acaggaccta
     5281 agaaaacaag ctacgccgaa ggggaggatt tggaccttag aggtggtgtt cttcgagttc
     5341 agtatgaagg aggaactgag gacgaactca ttcgcctaac tcacgcaggt gtatcagtat
     5401 caggttttga tacgcatcat aagggagaac agaatcttac tctccaatat ttgggacaac
     5461 cagtaaatgc taatttgtca gtgactgtca ctggccaaga tgaagcaagt ccgaaaacta
     5521 ttttgggaat tgaagtaagt caggaaccga aaaaagatta cctagttggt gatagcttag
     5581 acttgtctga aggacgcttt gcagtggctt atagcaatga caccatggaa gaacattcct
     5641 ttactgatga gggagttgaa atttctggtt acgatgctca aaagactggt cgtcaaacct
     5701 tgacgcttcg ttaccaaggt catgaagtca actttgatgt tttggtatct ccaaaagcag
     5761 cattgaacga tgagtacctc aaacaaaaat tagcagaagt tgaagctgct aagaacaagg
     5821 tggtctataa ctttgcttca ccagaagtaa aagaagcttt cttgaaagca attgaagcgg
     5881 ccgaacaagt gttgaaagac catgaaacta gcacccaaga tcaagtcaat gaccgactta
     5941 ataaattgac agaagctcat aaagctctga atggtcaaga gaaatttacg gaagaaaaga
     6001 cagagcttga tcgcttaaca ggtgaggctc aagaactctt ggctgccaaa ccaaaccatc
     6061 cttcaggttc tgccctagct ccgcttcttg agaaaaacaa ggccttggtt gaaaaagtag
     6121 atttgagtcc agaagagctt gcaacagcga aacagagtct aaaatatctg gttgctttat
     6181 tgaaagaaga caagtcagca gtcttttctg atagtaaaac aggtgttgaa gtacacttct
     6241 caaataaaga gaagactgtc atcaagggtt tgaaagtaga gcgtgttcaa gcaagtgctg
     6301 aagagaagaa atactttgct ggagaagatg ctcatgtctt tgaaatagaa ggtttggatg
     6361 aaaaaggtca agatgttgat ctctcttatg cttctattgt gaaaatccca attgaaaaag
     6421 ataagaaagt taagaaagta tttttcttac ctgaaggcaa agaggcagta gaattgactt
     6481 ttgaacaaac ggatagtcat gttatcttta cagcatctca ctttactcat tatgcctttg
     6541 tttatgaatc tgctgaaaaa ccacaacctg ctaaaccagc accacaaaac acagtccttc
     6601 caaaacctac ttatcaaccg gcttctgatc aacaaaaggc tcctaaattg gaagttcaag
     6661 aggaaaaggt tgcctttcat cgtcaagagc atgaaaatgc tgagatgcta gttggggaac
     6721 aacgagtcat catacaggga cgagatggac tgttaagaca tgtctttgaa gttgatgaaa
     6781 acggtcagcg tcgtcttcgt tcaacagaag tcatccaaga agcgattcca gaaattgttg
     6841 aaattggaac aaaagtaaaa acagtaccag cagtagtagc tacacaggaa aaaccagctc
     6901 aaaatacagc agttaaatca gaagaagcaa gcaaacaatt gccaaataca ggaacagctg
     6961 atgctaatga agccctaata gcaggcttag ccagccttgg tcttgctagt ttagccttga
     7021 ccttgagacg gaaaagagaa gataaagatt aaatatcgaa aaatcttgtg aaatctttcc
     7081 ttatatttcc aaagtgtgat ataatagttt cgaataaaat aaataaagga gtttttgtaa
     7141 catggcaaaa cttactgtta aagacgttga cttgaaaggt aaaaaagtcc tcgttcgtgt
     7201 tgacttcaac gtaccattga aagatggcgt aatcactaac gataaccgta tcacagcagc
     7261 tcttccaact attaagtaca tcatcgaaca aggtggacgt gcaattcttt tctctcacct
     7321 tggacgtgtg aaagaagaag ctgataaagc tggtaaatca cttgctcctg tagcagctga
     7381 cttggcagca aaacttggtc aagatgttgt tttcccaggt gtcactcgtg gtgctgaatt
     7441 ggaagcggca atcaacgctc ttgaagatgg acaagttctc ttggttgaaa acactcgtta
     7501 cgaagatgtt gacggcaaga aagaatctaa aaacgatcct gaacttggta aatactgggc
     7561 atcacttgga gatggtatct tcgtaaacga tgcattcggt acagctcacc gtgcacacgc
     7621 atctaacgtt ggtatctcag caaacgttga aaaagcagtt gctggtttcc ttcttgaaaa
     7681 cgaaattgcc tacatccaag aagcagttga aactccagaa cgtccattcg tggctatcct
     7741 tggtggttca aaagtttcag acaagatcgg tgttatcgaa aacttgcttg aaaaagctga
     7801 taaagtcctt atcggtggtg ggatgactta cacattctac aaagcacaag gtatcgaaat
     7861 cggtaactca cttgtagaag aagacaaatt ggatgttgcg aaagctcttc ttgaaaaagc
     7921 aaatggtaaa ttgatcttgc cagttgactc aaaagaagct aacgcatttg ctggttacac
     7981 tgaagtgcgt gacactgaag gtgaagcagt ttctgaaggc ttccttggtc ttgacatcgg
     8041 tccaaaatct atcgccaaat ttgacgaagc tttgactggt gccaaaacag ttgtatggaa
     8101 cggacctatg ggtgtatttg aaaacccaga tttccaagct ggtacaatcg gtgtgatgga
     8161 cgctatcgtg aaacaaccag gagttaaatc aatcatcggt ggtggtgact cagctgccgc
     8221 agcgattaac cttggccgtg cagacaagtt ctcatggatt agtacgggtg gtggagcatc
     8281 aatggaactt cttgaaggta aggttcttcc aggacttgca gccctgacag aaaaataaga
     8341 ttttataaac aaatcaaaga agagagggat gaaagttcct cttttctttt gcttaaaata
     8401 aaaacgcttc ctctcaacta ttactcataa aaatcaccga tttatgataa aatggaaata
     8461 gaaagttgag attatgagtt attttaaaaa atataaattc gataaatccc agttcaaact
     8521 tggtatgcga acctttaaaa caggtattgc tgtttttcta gttctcttga tttttggctt
     8581 ttttggctgg aaaggtcttc aaattggtgc tttgacagcc gtttttagcc tgagggagag
     8641 ttttgatgag agtgttcatt ttgggacttc gcgtattcta ggaaatagta tcggtggact
     8701 ctatgccttg gtctttttct tattaaatac ctttttccac gaagcctttt gggtgacctt
     8761 ggtagttgtt ccaatctgca ccatgttaac cattatgaca aatgtagcca tgaataacaa
     8821 agcaggggtt attggtggtg tagcagctat gttaatcatt accctatcaa ttccaagtgg
     8881 tgagacaatt ttgtacgtgt ttgtgcgtgt attagaaacg tttatgggag tttttgtcgc
     8941 aattatcgta aattacgata ttgatcgtat tcgtctcttt ttagagaaaa aagaaaaata
     9001 atgttacatt ttataacatt atcaattgac gtttgtcttt ttttagacta taatagacag
     9061 aaagaaggaa attgtaaatg aaggaaaaag aatttcgccg aaatatggct gtttttccta
     9121 tcggcagtgt tatgaagttg accgatttat cggcgcgtca gattcgttat tatgaagatc
     9181 aagagttgat caagcccgat cgaaacgaag gaaatcgtcg catgtattcc ttgaatgaca
     9241 tggatcgtct gcttgaaatc aaagattata tctctgaagg ttataatatc gctgccatta
     9301 agaaaaaata tgctgaacgt gaagcgaaat ccaagaaagc ggttagtcag actgaagtac
     9361 gtcgtgcact tcacaatgaa ctcctccaac aggggcgttt tgcttcagta cagtcacctt
     9421 ttggtcgcgg ttaggcaacc gcaagtagtc atagattaag gagaaaactt atgccaatca
     9481 cagctgcaga tattcgtcgt gaagtcaagg aaaaaaatgt tacctttatt cgtcttatgt
     9541 tctcagatat tttgggaacc atgaaaaacg tcgaaattcc tgctacagat gaacagttag
     9601 ataaggtctt gtcgaacaag gttatgtttg atggatcttc tattgaaggt tttgtacgta
     9661 tcaatgagtc ggatatgtac ttgtacccgg acttggatac atggacagtc ttcccttggg
     9721 gagatgaaaa tggaagtgtt gcaggtctga tctgtgatgt ctatacaaca gaaggtgaac
     9781 catttgcggg tgaccctcgt ggtaatttga aacgagctct tcgtcacatg gaagaagttg
     9841 gattcaaatc cttcaacctt ggtccagagc cagaattctt cctatttaag ttggatgaaa
     9901 atggggaccc aacacttgaa gtgaatgaca agggtggcta ctttgatttg gcacctactg
     9961 accttgcgga caacacacgt cgtgagattg tgaatgtctt gaccaaaatg ggatttgaag
    10021 tagaagcgag tcaccacgag gttgcggttg gacagcatga gattgacttt aagtacgatg
    10081 aagttctccg tgcttgtgat aagattcaaa tctttaaact tgttgttaaa accattgctc
    10141 gcaaacacgg actttacgca acatttatgg cgaagccaaa atttggtatt gctggatcag
    10201 gtatgcactg taatatgtcc ttgtttgatg cagaaggaaa taacgccttc tttgatccaa
    10261 atgatccaaa aggaatgcag ttgtcagaaa cagcttacca tttcctaggc ggtttgatca
    10321 agcatgctta caactatact gccatcatga acccaacagt taactcatac aaacgtttgg
    10381 ttccaggtta tgaagcgcct gtttacattg cttgggctgg tcgtaaccgt tcgccacttg
    10441 tgcgcgtacc tgcttcacgt ggtatgggaa ctcgtcttga gttgcgttca gtggatccaa
    10501 tggcgaaccc ttacgttgct atggctgttc ttttggaagt tggtttgtat ggtattgaaa
    10561 ataaaatcga agcaccagct cctatcgaag aaaatatcta catcatgaca gcagaagagc
    10621 gcaaggaagc tggtattaca gaccttccat caactcttca caacgctttg aaagctttga
    10681 cagaagatga agtggttaaa gctgctctcg gagatcacat ctacactagc ttccttgaag
    10741 ccaaacgaat cgaatgggca agttatgcaa ccttcgtttc acaatgggaa attgataatt
    10801 atttagagct ttactaatac taatatagaa gaaagattgc ctgtgggtaa acagtcacac
    10861 agcagtctaa gccatcaaat acctataacc cttaaaatag cgatttcggt cgctatttat
    10921 ttttcgaaaa atcctcttga ctcttttgcg aattagattt atcatcagac gtgtagaaga
    10981 aaattaagga gtcactatgg aaaaattcaa tttcaaaaac aatatcgggc aagaaaacaa
    11041 gttacttcaa atcgaaatct acaagtttac taacttttgc aagcttcaaa attacacatc
    11101 tgtaaatatt ttttcgaaag atatatttga aaccatagta aattgagact agaatagtac
    11161 actacatctg ctaaaaattt ctagaaacta atttaacttt cctaatcgat ttgtttacat
    11221 cttatttcaa tctactatat tatctcaata aatgatgatg gcgactgttt tgtttgtgct
    11281 tgtcgatttt tgaatcctga tcgagcggaa gaatatttct gccgaggttg ccaaggggaa
    11341 aatcctgaag atattgaatt ttatgatgaa caattacagg cagaaaaggt ggaggatttg
    11401 aatattcgat tggaagtaaa aaattgaatt atctacaatc tcatcaacaa taaacctatt
    11461 tcaaccccat tatctatagg cgtattttta cgccttttta tatacttgaa gcacataaga
    11521 aacattatgg gaagtttgct gatggaagta ctcaagaaat tgatgttcct tatgatattc
    11581 ctgatacttg ggagtgggtg aggttttcta cattggttga aattgtcaga ggtggctctc
    11641 cacgaccaat caaagattat cttacttctg aagtagatgg aataaattgg ataaaaatag
    11701 gtgatactga aaagggtgaa aagtatataa ataatgttaa agaaaaaatc aaaaaatcag
    11761 ggcttaacaa aactagattt gtaaaaaaag gtacattttt gttaactaat tctatgagtt
    11821 ttggtagacc ttatattttg aatgttgatg gtgcaataca cgatggatgg ttggctattt
    11881 cgaactatga aaactcatta aataaagatt acctattcta tattctttca tcaaatgtag
    11941 tttattctca atttctatct ctaattagtg gagctgttgt gaaaaacttg aatagtgata
    12001 aagttgcttc tattcttatc cctctccccc cactatccga acaacaacgc ataatagaag
    12061 caatcgaatc agctttagaa aaagtagatg aatatgctga aagttataat agactagaac
    12121 agctagataa agaatttcca gataaactaa aaaaatctat tcttcaatat gctatgcaag
    12181 gaaaattagt tgaacaagac ccaaatgatg aatcagtcga agttttactt gaaaaaatac
    12241 gagcagaaaa acaaaaactc tttgaagaag gcaagattaa aaagaaagat ttggacattt
    12301 ctattgtttc ccaaggagat gataactctt attatgagga agtaccttgt gaaataccag
    12361 aaagttggga atgggtgagg ttaaacgata taacctccta cattcaaaga gggaagtctc
    12421 caaaatattc aaatattcct atataccctg tgattgctca gaaatgtaat caatggtctg
    12481 gcttttctat tgacttagca agatttattg atcccgaaac agttcatagt taccagaaag
    12541 aaagattact aagagatggt gatttaatgt ggaattcaac aggtctaggt acgttaggac
    12601 gattagctat ttaccatgaa aataaaaatc cttatgtctg ggctgttgca gatagccatg
    12661 ttacagttat aagagtctta tcgggagtta ttaattgcca tttcatctat aattttcttt
    12721 cctctcctat tgttcagtca gttattgaag aaaaagcgtc gggtagtaca aaacaaaaag
    12781 aattattaac gaaaactata aaagaatatt taatccccct cccacccctt cctgaacaat
    12841 ccagaatcgt tgataaaatc gaacaatttt tcgcccatat tgacgcacta atttagtata
    12901 atagttttac gtgcggtgga atttctatca aacataagat aggagtttta cgttgaaaga
    12961 ttttgaatta catttgaaga aagctggttt agctgaaaat actgtgcgtt cgtaccttta
    13021 tggtgttcga tttttcttgg agaattacga actcaaaatg gaagatttat ttgaatacaa
    13081 aggatattta ttagacaatt ttaaacctaa aactgtcaat ttgcgactac aaggagtaaa
    13141 taaatatctg gcttttattg gtcatgatga tttgaaatta aaatttgtca aagtacagca
    13201 aaaaccgttt ttagaagatg tcattagcca tgctgattac ctttttctta aacgtagttt
    13261 gaaaaaagat ggtattttaa aatggcattt tgtcgtttga tttttaggtg caactggggc
    13321 tcgtgtaagt gaacttatta aactaaaagt agagcatgta gaaatcggtt attttgacat
    13381 ttactcaaaa ggtggtaaaa ttcgtagact gtacattcct aaaaaattaa gaaatagttg
    13441 tcttagttgg ttagaatcag aaaatcgccg aagtggttat ttatttttga ataaatttaa
    13501 cgaaccaatt acagcaagag gagttgctca acagttaaaa aattatgctg ataaatacaa
    13561 aatgaatcct aaagtaattt accctcattc ttttaggcat ttatttgcta agaatttttt
    13621 agcgaagtat aatgatattg ccttgcttgc agatttgatg ggacacgaaa gtatagaaac
    13681 tactcgaatt tatctaagga aaacagctac tgaacaacaa aatattgtag ataaaattgt
    13741 taattggtaa aaaataacag gtggtcaaac tgactacctg ctatttttgt gattatggct
    13801 cttattatgg gaata
//
LOCUS       DAVKLW010000049        13789 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKLW010000049 DAVKLW010000000
VERSION     DAVKLW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.049"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            29..298
                     /gene="blpJ"
                     /locus_tag="VW324_001570"
     CDS             29..298
                     /gene="blpJ"
                     /locus_tag="VW324_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9727176.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            367..615
                     /gene="blpK"
                     /locus_tag="VW324_001571"
     CDS             367..615
                     /gene="blpK"
                     /locus_tag="VW324_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEU9727177.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            859..1008
                     /gene="blpO"
                     /locus_tag="VW324_001572"
     CDS             859..1008
                     /gene="blpO"
                     /locus_tag="VW324_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9727178.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1112..1231
                     /locus_tag="VW324_001573"
     CDS             1112..1231
                     /locus_tag="VW324_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9727179.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            1699..2070
                     /locus_tag="VW324_001574"
     CDS             1699..2070
                     /locus_tag="VW324_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727180.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            2699..3082
                     /locus_tag="VW324_001575"
     CDS             2699..3082
                     /locus_tag="VW324_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9727181.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            3134..3823
                     /locus_tag="VW324_001576"
     CDS             3134..3823
                     /locus_tag="VW324_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9727182.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            3865..4098
                     /locus_tag="VW324_001577"
     CDS             3865..4098
                     /locus_tag="VW324_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727183.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            4249..4860
                     /locus_tag="VW324_001578"
     CDS             4249..4860
                     /locus_tag="VW324_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9727184.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            5021..5815
                     /locus_tag="VW324_001579"
     CDS             5021..5815
                     /locus_tag="VW324_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9727185.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5812..6447
                     /gene="trmB"
                     /locus_tag="VW324_001580"
     CDS             5812..6447
                     /gene="trmB"
                     /locus_tag="VW324_001580"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9727186.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6573..7052
                     /gene="rimP"
                     /locus_tag="VW324_001581"
     CDS             6573..7052
                     /gene="rimP"
                     /locus_tag="VW324_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9727187.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDGLTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            7096..8232
                     /gene="nusA"
                     /locus_tag="VW324_001582"
     CDS             7096..8232
                     /gene="nusA"
                     /locus_tag="VW324_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9727188.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            8254..8547
                     /locus_tag="VW324_001583"
     CDS             8254..8547
                     /locus_tag="VW324_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9727189.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            8540..8839
                     /locus_tag="VW324_001584"
     CDS             8540..8839
                     /locus_tag="VW324_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9727190.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8856..11648
                     /gene="infB"
                     /locus_tag="VW324_001585"
     CDS             8856..11648
                     /gene="infB"
                     /locus_tag="VW324_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9727191.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11899..12249
                     /gene="rbfA"
                     /locus_tag="VW324_001586"
     CDS             11899..12249
                     /gene="rbfA"
                     /locus_tag="VW324_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9727192.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            12528..13307
                     /locus_tag="VW324_001587"
     CDS             12528..13307
                     /locus_tag="VW324_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727193.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            13309..13491
                     /locus_tag="VW324_001588"
     CDS             13309..13491
                     /locus_tag="VW324_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727194.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            13451..13639
                     /locus_tag="VW324_001589"
     CDS             13451..13639
                     /locus_tag="VW324_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727195.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
BASE COUNT         4203 a         2341 c         3085 g         4160 t
ORIGIN      
        1 aattgaaaat aaaaaggagg tattcatcat gaatacaaaa atgttgtcac agttagaggt
       61 tatggatact gaaatgcttg cgaaagttga aggggggtat agctccactg attgtcaaaa
      121 tgcactgatt acaggagtca ctacgggaat tataactggt ggaactggag caggtttggc
      181 cactttagga gtagctggac ttgctggtgc atttgttgga gcacacattg gtgcaattgg
      241 gggtggatta acatgcttag gaggtatggt tggtgataaa ttgggactaa gttggtaaga
      301 cttatagtaa aattatgagg attctattct gatttatttt agttttttaa aaaaggagta
      361 tttgtcatgg atacaaaaat gatgtcacaa ttttctgtta tggatactga aatgcttgct
      421 tgcgttgaag gtggcggatg caattgggga gattttgcca aagcaggtgt tggaggagca
      481 gctgtagttg ctgctttagg ttgtgcagcg ggtggagtga aatatgggaa aattctagga
      541 ccatggggcg ctgcaatagg aggaattgga ggagcagtgg tttgtggata tttagcctat
      601 accgctacat cataaaaatg aaagctaaat tttatgaagg ctatattctt tattattctc
      661 ttcgcttttc aaacctatct tatttatcta tccataagta tctctgataa aaaacaaaag
      721 actattgaat taacatcatt gaattgtttt gtcatattat ttctaatcta tgataaatta
      781 atttttctct ttattgccta tgtttttttg ataattttta tattaaactt gttccgtaac
      841 tgaaaagtga catactatat gaatacaaaa atgatgtcac aattttctgt tatggataat
      901 gaaatgcttg cttgcgttga aggtggagat attgattggg gaagaaaaat tagttgtgca
      961 gcaggggttg catatggcgc aattgatggg tgtgcaacaa cggtttgaca tttctattgg
     1021 gaccatttgc tataggaata ggtgtaactg gtgctgcagg tggagctata cttggaggtg
     1081 tagcctatgc agcaacatgt tggtggtaat tatggatttt aaaagtttta ttattggttt
     1141 agtagttggt atatttggtc cttatatgga tgacttaatt agaaaaatat tttcaaaatc
     1201 ttccaaaaag gatacggata gtactctcta aaaaataaat ttctcccctg tatttgctag
     1261 cttaatttac aggggagttt ctttaatatt gtttcgcctg tcaaaatgat atgtgatagc
     1321 aggatgaaaa gtctacaaac aagttggtat caatacaata taaagaggaa agtatgtttt
     1381 tcatgcttgc ttttttaatt tttacaattc aagaggtttt gatgaccatt tatgatttga
     1441 gtgatccaag gtcaaaataa gtgctatact agcagtgtaa atgttcttgc tcaactaaga
     1501 taaatcgcac atttaggagg aaaacttatg aagaaaaaaa tactgattat tttcgttctg
     1561 tatctgatca tgtccatctt tctttatccg cttagggaga gtatttggta taatctattt
     1621 tataccatag cctatatgat tgcggttatg atctattttt ctttaattaa aaagaaagaa
     1681 aagaaatgaa agattttctt gattaaaaag aatcatattc tttgtgagat tgttcctggg
     1741 tcagttgcct ttgcttgttt ctacttatct atttctattt cgtcagtttt ccaatttctt
     1801 ttagtagtta ttaacttgac ttctattttg gttactgttt atcttactag ggaaatgaga
     1861 ttaagagagt ttgaagatga tgatttggtt agtcctagaa ccaatcaact catgtttatc
     1921 ggcttgacag gctttatgtc tattatttgt ttgtatagag gtatcacagc aggagaatct
     1981 tatcaacaac taatcgctta tattggtgct attctttgct tgatcatcat gcttctactc
     2041 atttggggtt tgaagtatta taaaaagtag gggctaatga agtttttgca atttaggagg
     2101 tttcgatgac aattcaagat ttggatgaaa aattttaaaa aacaatgata tactaaactt
     2161 gtcaaagttg caacgggtgc tgctacatta tgttaaaaat cgctcttgat tgataagttg
     2221 gatttgttag gaaaggagta aataattatg gataagaaaa agatagtttc gactataata
     2281 tgtatagtat ttcttgtagt gtcagtagat aattttttta gagacttaac cccgttactg
     2341 ttcattttaa atattattgg cctgtcttgc ttttcagtgc taacatacat taacataaaa
     2401 gagatactct taaatataag taaatgatga tttggttagt cctagaacca atcaacttat
     2461 gtttatcggc ttggcagatt ttatgtttgt catttgtttg tatagaggta tttcagaaac
     2521 agaattctat caacaactaa tcgcttatat aggtgtattc tctgcttgtt tatcacgctt
     2581 ctgctcatgt ggggcttgaa gtattataaa aagtagcttt ctatcctttt ctgacaggat
     2641 atttgagtag aaggctctat taaattgtta gtgaaataaa tgaatggagg tatttaatat
     2701 gaaatacaga ttattttttg ttattttctt gagtagtgtg ttggatattc ttttagggac
     2761 atttttacaa atctctatcg tatctattgg gtggcttgtt ctttacagtg gtttgtttga
     2821 agcaggagtt ttccttcttg ctaataaagg ggtggcggta aaaatcaagg aagtagatat
     2881 tcgaaatcgc tttaaattta tttttggaaa aaccttatgg tttcaaattc ttttgctcat
     2941 ctttttgata atcaaacttt atcttggttt ggatgcgagg ttgattttat tctatggaca
     3001 tattttcatt gtctttaatg ccttaatgta tctattatct agtagtcagg ttagccttaa
     3061 aaaaaacaaa ctgtcttctt aatcttacag cagtagagcc tgacaaggtg aggtcatagt
     3121 agggaggatt tctatgaaaa agtatcaact tctattcaaa ataagtgcag ttttctctta
     3181 cttatttttc gtatttggtc tttctcagat gacgcttatt attcaaaatt attggcaatt
     3241 ttcttcccag attggcaatt tcgtctggat tcaaaatttc ttgagtttgc tatttagcgg
     3301 agtcatgatt tggattctgg ttaagacagg tcatggttat ctctttcaca ttccaagaaa
     3361 aaaatggctt tggtattcga ttttgacagt attagtggta gtgctccaga tctcttttaa
     3421 cgttcagaca gctaaacatg ttcagtcaac tgctgaaggt tgggctgtat tgatcggtta
     3481 tagtgggacc aactttgctg agctaggtat ctatataact ttgttctttc tgactccact
     3541 tatggaagag ctaatctata gaggattact gcaacacgcc ttttttaagc attcgagatt
     3601 tggccttgat ttgcttcttc cgtcaatttt atttgctctt cctcattttt caagcctgcc
     3661 tagtttgtta gatatcttca tctttgcaac atctggcatc atctttgcta gtttgacccg
     3721 ctataccaag agcatttatc cttcctatgc ggtgcatgtg atcaataata ttttcgcaac
     3781 attaccattt ttgctgactt ttttacatag ggtcttgggg taaaaaaaca aaaggcttgc
     3841 ttttcagcca tagaggagat tatcatgtat aaacacttat ttttcctaga ttccaaaact
     3901 ttagatcggt tgacacccta tattctagtc ttggcttctg acaccattgc ttttaatgtt
     3961 tttgtgctaa cctttgtatc tgcggtggtt tttaatttcc taaattccat gctagcttta
     4021 atggctatat tcataggggc tggctatgtg gtcggatttt ggttactaat actcaatgaa
     4081 aatcaaagag caaactagga aactagccgc aggctgtact tgagtacggc aaggcgacgt
     4141 tgacgtggtt tgaatttgat tttcgaagag tataaaatgg tttgttttgg aaaggctaga
     4201 actaaagaat gacgcgtagg aagtctgttt ggtgaggagg atagttttat ggagtttttt
     4261 gataaatttc atgccttgtg ttttggattt ttagtactaa taattgtcat tacagttcct
     4321 tatacgatta accatggggg tttttttcaa aatgaatctg cattgattct tgtaagtctt
     4381 cttgtaacct cgctgagtgt tgcttatgct agaaagtttg aaatgatttc ttttgggatg
     4441 ttaagcaaga aacaactttt gcttttcatt gcaatctttc ttctaagtgt acttgagacg
     4501 ctggtttata ttcatttctt cgctgtttct tctggctcag gggtccaaca cttggcggaa
     4561 gtcagcagag gaatttccct gtctttgatt ttgactacct cagtttttgg ccccatccag
     4621 gaggaactca ttttcagagg acttcttcaa ggtgcggttt ttgacaattc ttggttaggg
     4681 cttgtgctaa cttcctctct cttttctttc atgcatggac cttctaatgt cccttcgttt
     4741 attttttatc tacttggggg tttgttgctg ggctttgctt ataaaaagag tcaaaaccta
     4801 tgggtttcta ctctagtcca catgttttac aacagttggc cactcctata ttatttataa
     4861 aaattatgaa aaggtaagca gaagatggtg cttacctttt tcaagctata atgataccca
     4921 agccaatcca gaggcttttc tttgagaatc gggttagagt ggttgacgaa taggccaaaa
     4981 actagtagaa tagtaaggaa actttatacg gaggaaagaa atggatttgg gtgataatga
     5041 gctaacactg actcccatac ctgggaaaag tggcaaggct tatatgggta gctatcctga
     5101 cgggaagcgc atctttgtaa aaatgaacac ctctccaatc ctacctggtc tagctagaga
     5161 acaaattgct ccacaattat tatggagtcg tcgtttggca gatgggcgtg atatgtgtgc
     5221 tcaagaatgg ttgacaggca agatattgac cccctatgat atgaatcgta agcaaatcgt
     5281 caatatttta acccgtcttc atcgctcacg tccgttgatg acacaattga gtcgtttggg
     5341 ctatgccatg gaaacacctg tagatttact ccagtcttgg caggaaacgg ctccagatgc
     5401 tttgcgtaaa aatcatttta tcagtgaagt gatggctgat ttacgtcaga ctattccagg
     5461 atttagagag gaccatgcga ccattgtcca tggagatgta cgacatagta attggattga
     5521 gacagatagt ggcttgattt atttagtaga ttgggattcg gttcgcttga ccgatcgcat
     5581 gtttgatgtg gcccatatgc tctgccatta tatttcagaa catcagtgga aggaatggtt
     5641 gacctactac ggttacaagt acaatcaaac ggtattaagt aaattgtatt ggtatggtca
     5701 attgtcttat ttgagtcaga tttccaagta ttatatgaac caagatttag aaaatgtcaa
     5761 tcgggagatt catggtttgc gtcatttccg agacaagtat ggaaagagaa gatgagagtt
     5821 agaaatcgta aaggggcaac agaattacta gaggcaaatc cccagtatgt ggtcctcaat
     5881 cccttggaag ccaaggcaaa atggcgggac ttgtttggca atgataatcc cattcatgtg
     5941 gaagttggaa gtggaaaggg tgcctttgtt tcaggtatgg ccaagcaaaa ccctgacatc
     6001 aactatatcg ggattgatat tcaaaagtct gttttgagct acgctttgga caaggtgctt
     6061 gaagttggag tgcctaacat caagctcttg tgggtagatg gttctgactt aactgactat
     6121 tttgaagacg gtgagattga tcgcttgtat ctgaactttt cagatccatg gccgaaaaaa
     6181 cgccatgaaa agcgtcgttt gacctacaag accttcttgg ataccttcaa acgtatcttg
     6241 cctgaaaatg gagaaattca tttcaagacg gataaccgtg gcttgtttga gtacagttta
     6301 gtgagctttt ctcaatatgg catgaaactc aatggtgtct ggttagattt gcatgccagt
     6361 gattttgaag gcaatgtcat gacagaatac gagcaaaaat tctcaaacaa ggggcaagtt
     6421 atctaccgag ttgaggcaga attttaagag atagcctaaa attaggctgt acaagtgctt
     6481 ttgctttaca taagttggca aacgtgctat actgatagta agaatatgaa aagtgaggcg
     6541 gggaaatatc ttcgcctctt gcttatgagg aggtggacgc aatcgcaaca atcgtagaat
     6601 tagtcagaga agttgtagaa cctgtcatag aagctccttt tgaactcgtg gatatcgagt
     6661 atggaaagat tggcagtgac atgattctca gtatttttgt agataaaccc gaaggaatta
     6721 ccttgaacga cacggcagac ttgacagaaa ttatcagtcc tgtcctagac accatcaagc
     6781 cagatccctt cccagaacaa tatttcctag aaattaccag tccaggtttg gaacgccctt
     6841 tgaaaaccaa ggatgccgtc gctggagcgg ttggaaaata catccatgtc gggctctacc
     6901 aagccatcga taagcaaaag gtctttgaag gaaccttgtt ggccttcgaa gaggacgggt
     6961 tgactatgga atatatggac aagacgcgta agaaaaccgt ccaaattcca tacagtttag
     7021 tatcaaaagc acgtttagca gttaaattat agaaaaagaa aggatagctt ttgaggattc
     7081 aaaagtgaag aaaacatgag taaagaaatg ctagaggcct tccgcatttt ggaagaagac
     7141 aagggaatca aaaaagaaga tatcatcgac gcagtagtag agtcgcttcg ttccgcttat
     7201 cgcagacgct atggtcagtc agacagcgta gctattgact tcaacgaaaa aacaggtgac
     7261 tttacagttt atactgtccg tgaagttgtt gatgaagtat ttgatagccg tttggaaatc
     7321 agcttgaaag atgctcttgc cattaattca gcttatgaac ttggagacaa aatcaagttt
     7381 gaagaagcac cagctgagtt tggtcgtgta gcagcccagt ctgccaaaca aaccatcatg
     7441 gaaaaaatgc gcaagcaaac acgtgccatc acttacaata cttacaaaga acatgagcaa
     7501 gaaatcatgt ctggtacagt agaacgcttt gacaaccgct ttatctatgt caaccttggt
     7561 agcatcgaag cccaattgtc aaaacaagac caaattcctg gagaagtttt tgcttctcat
     7621 gatcgtatcg aagtttatgt ttacaaggtt gaagacaacc ctcgtggtgt gaacgtcttt
     7681 gttagccgta gtcatccaga aatgatcaaa cgtttaatgg agcaagaaat tccagaagtt
     7741 tatgatggaa ctgttgaaat catgagcgtg gctcgtgaag caggtgaccg tacgaaggtt
     7801 gctgttcgta gccacaatcc aaacgtggat gctatcggta caatcgttgg acgtggtggt
     7861 gctaatatca agaagattac tagcaaattc cacccagctc gttacgatgc taaaaatgac
     7921 cgcatggtac caatcgaaga aaatatcgat gttatcgagt gggtagcaga tccagctgaa
     7981 tttatctaca atgccatcgc tcctgctgag gttgaccaag ttatctttga tgaaaacgac
     8041 agcaaacgtg ccttggtggt tgttccagat aacaagcttt ctcttgccat tggtcgtcgt
     8101 ggacaaaacg tgcgcttggc ggctcacttg actggttacc gtatcgatat caagtctgct
     8161 agcgaatttg aagccatgga agacgctgct tcagtagagt tggaagtaga aaacgatact
     8221 gtagaagaat aaaagctgct agaggaggga aagatgaaaa caagaaaaat ccctttgcgc
     8281 aagtctgttg tgtctaacga agtgattgat aagcgtgatt tgctccgcat tgtcaagaac
     8341 aaggaaggac aagtctttat tgatcctacg ggcaaggcca atggccgcgg cgcttatatc
     8401 aaactagaca atgcagaagc cctagaggcg aaaaagaaga aggtctttaa ccgcagcttt
     8461 agcatggaag tggaagaaag cttttatgac gagttgatcg cttatgtgga tcacaaagtg
     8521 aaaagaagag agttgggact tgaataagca aaagataagt aatctcttgg ggcttgctca
     8581 gcgagcaggg cgcatcatat cgggtgaaga attggtggtc aaggccattc aagacggcaa
     8641 ggccaagttg gtctttctag ctcatgatgc tggacccaat ctgaccaaga agattcaaga
     8701 taaaagtcat tattatcaag tagaaattgt aaccgtgttt tcaacactgg aattaagcat
     8761 agcagtcggg aaatcgagaa aggttttggc tgtaacagat gctggattta caaagaaaat
     8821 gaggtctctt atggaataga agaggaggac atgatttgtc taagaaaaga ttgtacgaaa
     8881 tcgcaaaaga acttggaaaa gaaagtaaag aagttgtagc gcgtgcaaaa gagttgggct
     8941 tggatgtgaa aagccactca tcaagtgtgg aagaagctgt cgctgcaaaa attgctgcca
     9001 gctttaagcc tgcagctgct ccgaaagtag aagcaaaacc tgcagcacca aaagtaagtg
     9061 cagaaaagaa agccgaaaaa tctgagccag ctaaaccagc tgtagctaag gaagaggcaa
     9121 aaccggctga gccagttgct ccgaaaacag aaaaagtagc agtgaaaccg caaagtcgta
     9181 atttcaaggc tgagcgtgaa gcccgtgcta aagagcaggc agaacgacgc aagcaaaata
     9241 agggcaataa ccgtgaccaa caacaaaacg gaaaccgtca gaaaaacgac ggccgtaatg
     9301 gtggaaaaca aggtcaaagc aaccgcgaca atcgtcgctt taatgaccaa gctaagaagc
     9361 agcaaggtca gcaaaaacgt agaaatgagc gccgtcagca agaggataaa cgttcaaatc
     9421 aagcggctcc acgtattgac tttaaagccc gtgcagcagc cctaaaagca gagcaaaatg
     9481 cagagtacgc tcgttcaagt gaggaacgct tcaagcagta tcaggctgct aaagaagcct
     9541 tggctcaagc taacaaacgc aaggaaccag aggaaatctt tgaagaagcg gctaagttag
     9601 ctgaacaagc acagcaagtt caagcagtgg ttgaagtcgt ccctgagaaa aaagaacctg
     9661 cagtggatac acgtcgtaaa aaacaagctc gaccagacaa aaatcgtgac gattatgatc
     9721 atgaagaaga tggtcctaga aaacaacaaa agaatcgaag tagtcaaaat caagtgagaa
     9781 atcaaaagaa tagtaactgg aataacaaca aaaagaacaa aaaaggcaat aacaagaaca
     9841 accgtaatca gactccaaaa cctgttacgg agcgtaaatt ccatgaattg ccaacagaat
     9901 ttgaatatac agatggtatg accgttgcgg aaatcgcaaa acgtatcaaa cgtgaaccag
     9961 ctgaaattgt taagaaactt ttcatgatgg gtgtcatggc cacacaaaac caatccttgg
    10021 atggggaaac aattgaactc ctcatggtgg attacggtat cgaagccaaa caaaaggttg
    10081 aagtggataa tgctgacatc gaacgtttct ttgtcgaaga tggttatctc aatgaagatg
    10141 aattggttga gcgtccacca gttgttacta tcatgggaca cgttgaccac ggtaaaacaa
    10201 cccttttgga tactcttcgt aactcacgtg ttgcgacagg tgaagcaggt ggtattactc
    10261 agcatatcgg tgcctaccaa atcgtggaaa atggtaagaa gattaccttc cttgatacac
    10321 caggacacgc ggcctttaca tcaatgcgtg cgcgtggtgc ttctgttacc gatattacga
    10381 tcttggtcgt agcggcagat gacggggtta tgcctcagac tattgaagcc atcaaccact
    10441 caaaagcagc taacgttcca atcatcgtag ccattaacaa gattgataaa ccaggtgcta
    10501 acccagaacg cgttatcggt gaattggcag agcatggtgt gatgtcaacc gcttggggtg
    10561 gagattctga atttgttgaa atctcagcta aattcaacca aaatatcgaa gaattgttgg
    10621 aaacagtcct tcttgtagct gaaatccaag aactcaaggc agacccaaca gtgcgtgcaa
    10681 tcggtacggt tatcgaagcg cgcttggata aaggaaaagg tgcggtcgca acccttcttg
    10741 tacaacaagg taccttgaat gttcaagatc caatcgttgt cggaaatacc ttcggtcgtg
    10801 tccgtgctat gaccaacgac cttggtcgtc gtgttaaagt tgctggacca tcaacaccag
    10861 tctctatcac aggtttgaac gaagcaccga tggcgggtga ccactttgcc gtttacgagg
    10921 atgaaaaatc tgcgcgtgca gcaggtgaag agcgtgccaa acgtgccctc atgaaacaac
    10981 gtcaagctac ccaacgtgtt agccttgaaa acctctttga tacccttaaa gctggggaac
    11041 tcaaatctgt taatgttatc atcaaggctg atgtacaagg ttctgttgaa gccctttctg
    11101 cctcacttca aaagattgac gtggaaggtg tcaaagtgac tatcgtccac tcagcggtcg
    11161 gtgctatcaa cgaatcagac gtgacccttg ccgaagcttc aaatgccttt atcgttggtt
    11221 tcaacgtacg ccctacacca caagctcgtc aacaagcaga agctgacgat gtggaaatcc
    11281 gtcttcacag cattatctac aaggttatcg aagagatgga agaagctatg aaagggatgc
    11341 ttgatccaga atttgaagaa aaagttattg gtgaagcggt tatccgtgaa accttcaagg
    11401 tgtctaaagt gggaactatc ggtggattta tggttatcaa cggtaaggtt gcccgtgact
    11461 ctaaagtccg tgttatccgt gatggtgtcg ttatctatga tggcgaactc gcaagcttga
    11521 aacactacaa agacgacgtg aaagaagtga caaacggtcg tgaaggtgga ttgatgatcg
    11581 acggctacaa tgatattaag atggatgatg tgattgaggc gtatgtcatg gaagaaatca
    11641 agagataaga ttttttgctc ctttcttagg tggtgaggga cgcaagcaaa ccgatggttt
    11701 cattgcttat ttttgagcct agggtctcaa aaatcccctg tgatgggact gataaatcag
    11761 ttccatcact ttcaccacgg cgaaagaagc agatgacttc aaattgaact tcgtttcaat
    11821 ttaaactgaa aatcaagaag tttaaaatag ctaggtctgc tggcctagct tttggttcaa
    11881 agtagagaaa ggaatatcat ggcaaatcat ttccgtacag atcgtgtggg catggaaatc
    11941 aagcgtgaag tcaatgagat tttgcaaaag aaagtccgtg atccacgtgt ccaaggtgtg
    12001 accatcacag atgttcagat gctgggtgac ttgtctgttg ccaaggttta ttacaccatt
    12061 ttgagtaacc ttgcttcgga taaccaaaaa gcccaaatcg ggcttgaaaa agcaactggt
    12121 accatcaaac gtgaacttgg tcgcaatttg aaattgtaca aaatcccaga tttgaccttc
    12181 gtcaaagacg agtccatcga gtatggaaac aagattgacg agatgctacg caatctggat
    12241 aagaactaaa gaagaggggt tgcccctctt ttttggtgga ggaaaatagg ttgaatttga
    12301 aatggaaaaa tattctttta aaatatattg aaactagaat agtacgcctc tacttctaaa
    12361 acattgttag aaatcgattt gactgtcctg atcgatttgt cctgttcttg tttcatttta
    12421 ctataaaaaa ggggttctgt attttttaat gttatctaat tagaaaatgc tttttttgta
    12481 ggaaatataa tatgataagg tgtaaaaaag aaataaggag tttgtatatg gctgaacaag
    12541 acttagctat gcaagtattg caacaagtgg tgaaactacc tgttgttaag gttgatcgtt
    12601 cgaaattttt agtggataag ttttccaaag aattggatcc aaaagatatt cctaccttat
    12661 tggaacaagg tacaacgact cttctatctc aagaaatatt agatcgtgta gctaatgctt
    12721 gtattcggga caatgtatta ttagcgagtg ggacttctgt tttggcagga ttagctggag
    12781 ggcttgctat ggcaattacc attccagctg atgtggctca attttatgct ttctctctga
    12841 aattggctca agaattaggt tatatttatg gttatgagga tctttgggct tcacgcgagg
    12901 agttgagtga agatgctcaa aataccctct tgctttatct aggcgtaatg ttaggggtga
    12961 atggaaccgc tgctttgcta tgtgctggtg gtataacaat tgccaaacag gtaatgaaaa
    13021 cagtgcctaa taaagcttta acaaagacgc tttggtaccc tattttgaaa aaagtcttaa
    13081 aaatatttgg tgtgaatctt accaagggag ggctggccaa aggaataggg aaatttattc
    13141 ctatcttggg tggtatcatt gcaggtggtt taacctttgc aactatgaaa ccaatggggg
    13201 aaagcttgca gaaagaatta tccaagctag tcaactatag tgaagttcaa tatcaagaag
    13261 atgttgaaac aatccgaaaa gaggctgaaa tcattaaagg agagtaatat gaatcctatc
    13321 aaagcttttg ataaaattta tggtaattac tttttgaccg tgcaaggtgt aaaagtgatg
    13381 aaaacgataa agaaagctga ccatgtcgtt gttggtctgg gaaaactttt tattgcagat
    13441 aagctaatgg atacggctcg gtggctcatt aggccagagg agagagaatg aaattttttt
    13501 tggtcttctt gctattcttt ttatcaaacc gattattggg attgtgaaat tcttttggat
    13561 gatcatttcc tttgcagttc aattgctgtt ttacaagata ttatttaaga tattggattg
    13621 gctctttaaa cttatctaga tggtaatcca agtcgcagag agctagcagg aactccactg
    13681 ctagtttttt aatctctctc catatggtat aatataagca gtaaaatcat tttatactct
    13741 tcgaaaatct cttcaaacca cgtcagcttc accttgcagt atatatgtt
//
LOCUS       DAVKLW010000050        13724 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKLW010000050 DAVKLW010000000
VERSION     DAVKLW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.050"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(177..1592)
                     /locus_tag="VW324_001590"
     CDS             complement(177..1592)
                     /locus_tag="VW324_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9727196.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1605..3443)
                     /locus_tag="VW324_001591"
     CDS             complement(1605..3443)
                     /locus_tag="VW324_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9727197.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3461..4300)
                     /locus_tag="VW324_001592"
     CDS             complement(3461..4300)
                     /locus_tag="VW324_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9727198.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4600..7158)
                     /locus_tag="VW324_001593"
     CDS             complement(4600..7158)
                     /locus_tag="VW324_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9727199.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
     gene            7706..8906
                     /locus_tag="VW324_001594"
                     /pseudo
     CDS             7706..8906
                     /locus_tag="VW324_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9021..9824)
                     /locus_tag="VW324_001595"
     CDS             complement(9021..9824)
                     /locus_tag="VW324_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9727200.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(10107..10862)
                     /locus_tag="VW324_001596"
     CDS             complement(10107..10862)
                     /locus_tag="VW324_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9727201.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(10932..13583)
                     /locus_tag="VW324_001597"
     CDS             complement(10932..13583)
                     /locus_tag="VW324_001597"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9727202.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(13595..>13724)
                     /locus_tag="VW324_001598"
     CDS             complement(13595..>13724)
                     /locus_tag="VW324_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9727203.1"
                     /translation="KYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
BASE COUNT         4332 a         2900 c         2188 g         4304 t
ORIGIN      
        1 ataaccaatc tatcctaagt taactattta taatcggctc tctgtaacaa aattaaatta
       61 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ctctttattt
      121 catatttaat gagatgctgc cttaaccgtg ataatatcta atcatcaata aacaaactat
      181 ttgaataagg attctccatt tgattcaatc actcctttat accaagtaaa ggacattttc
      241 ttatatcgat tgaatgtgcc atttccatca tcatttcgat caacataaat gagaccgtac
      301 ctcttagaaa gctgtgcagt ggacatagaa acgcagtcaa tacatcccca agacgtatag
      361 cccataattt ccacaccatc ttgtagagct tcagctactt gcaataaatg ttctttcata
      421 tactgaattc tataatcatc ttggactgtt aagttattaa attcatcttt tattagttga
      481 tctttagcac ctaatccgtt ttccactata aataatggaa tttgataacg gtcataatat
      541 ctatttaaaa ttatacgtag tccaattgga tcaatttgcc atccccactc agaagactct
      601 aaataaggat ttactaaacc accaataata ttcccttctc ctgaattata ctgtgttgga
      661 agagcagatt gagtcacact catgtaatag ctaaaggata aaaaatctac agtataattt
      721 tttaataact ctgcatcttc agctgcaaac tctatgttaa tgtcattttc cttaaaatat
      781 ctttttgcat aattcggata ataacctcta acatgcacat ctgaaaatag ataatttaga
      841 ttctcatact catgagtcgc ccatacatct tttggatttg gagtcattgg ataagctggc
      901 atagctaata ccatacaccc caccttaaac tctgaattaa tctcacgagc aatttttgta
      961 accaaactcg aggcgactaa ttcatgatgt atagcttgat ataattcttg tttcgaaaga
     1021 ttctccttag gtatatctat tcctccacta gtaaacggta attccaaaac agagtttact
     1081 tcgttaaatg taagccaata tttaacttta tctttatacc tttctaaaac tgttcgagca
     1141 aatttttcat aaaaatgaat cattctccta tcaatccatc catgatattt tcttgctaaa
     1201 tataatggag tctcatagtg tgaaagagtt acaagtggtt ctatcccgtg agcatgtagt
     1261 tcatcaaaca attcatcata atatttcaac ccagcttcgt taggttcttc ctcatctcct
     1321 tttggaaaaa ttctactcca tgcaatagaa gtacgaaaaa cattaaagcc catttcagaa
     1381 aacaaggata tatcttcctt atatttatga taaaaatcaa tacctatcaa ttttaagtta
     1441 tcttctgtag gattttctgt tgcttctcct aatccacctt tgggtaacac atcctgaact
     1501 gataagccct taccatcttc attatatgct ccctctactt gattagctgc aacagctcca
     1561 ccccaaagaa aatcatctgg aaaaatggtc ataactttcc tccattataa tattaccagt
     1621 aattccttag aatgctcgat tgtctgatta ttaggtaata ctaatacatc tagaaaatca
     1681 ttggtattcg ttacaattac tggtgtaact gtttcgtagc ctttagtctt gattaaattc
     1741 aagtccattt caaaaatcaa ctgatttttg aaaactctgt ctccttcttc tacatgacta
     1801 ataaaacctt gaccttttag ctcaacagta tctaatccaa tatgaattag taactcaaca
     1861 ccctcatcac tcttcaatcc aattgcgtgc ttagtcggaa aaatatttgt aattttccca
     1921 tcaaatggtg cataaacctt accttcactt ggaataatcg ctactccgtc tccaattagt
     1981 ttatctgaaa atgttttatc ctggacatcg cttaacggaa tgatttctcc tgatatagga
     2041 gaaaatatca tttttttatt tgaaactcca gctttaactt ctaaattgct agaactctct
     2101 tcttcatcga ttccaaatat ataagctaat acaaaggtaa taacaaccga aataaccgcc
     2161 acaattaaag catttacaat atttgatggc acatcagaat aaataaattg aggcaacgct
     2221 atcaaagatg ggacagcaaa tagatacgct ttaacactag taagacctgc aaataatccc
     2281 gctaatccac caccaatcat agctgcataa agcggttttt tatattttaa agtcacacca
     2341 tataatgcag gttcggtaat ccctgcaagt aaggctgaga aacctgctgc aaaagcaatt
     2401 tgttttgtat tattattttt actctttaat gcaacagcca tcgaagcagc cccttgagct
     2461 aagtttgacc ctaacattgc tggaagaatt aatacgtctg gagtagcaat agatgccgcc
     2521 aaaaaaatag gtgcaaaagc ccaatgcatt ccagtcataa caataaatgg cataatagca
     2581 ccaagaatag ctaatgtaag ccatccagct acaccataca tttgcccaac tagatttgat
     2641 aatccttcac caacaattac tccaataggc ccgactacaa ctaaggcaat acagcttgat
     2701 actaataata ctagcgtagg ttgcaaaaaa ctcttagtaa cagctggtgt taatttagca
     2761 atcatttttt caatatattt catcaaccaa accataataa gaattggaac gactgacgaa
     2821 ccataactag ctggtgtcac aggtacacca aataaactaa gaggattccc tgattgcacc
     2881 atttgaacaa aatttggatg gagaagtaca cctgctacag acatagctaa tgtagatgtt
     2941 acttttaatt tttgtgatgc agaataagct aataacagcg gtaagaaata atatggagca
     3001 tccccaaaaa atgtcaaaaa agcaatagtc tgagaatctg attgcaatat accaagcatt
     3061 ggtaaaataa ttaccaagac tttcagcata cctcccccta acattgctgg aatgattgga
     3121 gtcatggaac cagcgatata ctcaatgatt ctttctaaaa tattcccttt gtgcccttga
     3181 acaactgaat cggattcaaa attgccaagt ttaacgaatt ctttataata attagctaca
     3241 tcattaccaa gtataatttg atattgtcca ttctttttca taatacctat tacacctggt
     3301 atcttcttca catcatcatc attgactaaa ttttcatctt ttaattctaa tcttaaacgt
     3361 gttacacaat gggtaactct attgacattt ttttcacctc caattacatc gaggattttt
     3421 tgtaccgtat ctttataact catggtattc tcctattcta ttaatctaaa ttttttgtta
     3481 agcgacgaat atgagccatc aaataaacta attcactaga agtcagcaaa taattgtact
     3541 ccgtttgtat aaacattgcc acctgttcac cacattcata ttctctagga tatttatttt
     3601 tcattaatgc taacaagtct tcatcatcat cgtcacaata agttgtattt tcaaccaagc
     3661 gatgggcaaa taattttata tgagttatga atctataata atcaatagaa tcttggttaa
     3721 actcagtaca atagaaatct tgaatgattt tctcaattcc cataattatt tcagtgattt
     3781 tatacgactc ttgaaaatta ttttctaaat tagcattaac aaaatgtagc gctatgaatg
     3841 cagcttcgtc aattggaagt tcaataccta actctttttt tattatttgt aaagcccttt
     3901 tcccaagttc aaattcttct ttataatatt tcgatatttc ccagcgtagg ggattcgaaa
     3961 taataatccc ttctttataa cgttctatgc tcgaagaaat atgatccgtt aaattaatat
     4021 agataatttc atcaaaatta ttacccaatg ttattttacc taggttaatt attttttcgg
     4081 tgatgtacac caactccaaa ggaacgttaa taaacatctc tgtaaaacgg gtcatattat
     4141 ctctattttt gagaaaaaaa cttttctcaa tatcaattgg atttacttta tctccagttt
     4201 ttcttccaaa agctattccc cttccaaaaa gaagaatatc gatatctttt ttactttgcg
     4261 caattacggc attgtggttt agtattcgtt ttatgatcat aaaatttccc ttttaaaata
     4321 aaaggaacct acttaaaaac atcaagaaac ttcttctgat gtgattaaat aggttcctgc
     4381 tcacttgtat tagtgataac atccttttat ttcaattttt aatggtgaca tgaagcttaa
     4441 ataattaata actctgtaac cgtttttatt ttatgatatt tcaatctatt tgtcaagtac
     4501 ttataaaaat ttttttattt tgtgtgattc gaatgtgata atggcaacag tctcactaaa
     4561 gaaatgaaaa tcatacatag aaatttttaa aaattttctc tatcgtaaac ctgccactag
     4621 ttcagaaact atactagctg attccaaacg taatatggtc atctcatcta ccttccctaa
     4681 taatggcatt gcaccatacc aaacaatagt cttattcacc aaaagaatag gcgttactat
     4741 ttttgattga gtaaaagaca ctttaatttt ctggctcttc aactggtcta gccatgcttt
     4801 atttgcaacc gtctctggta tacatatctc aatttgacta atcctaaatt cttttagaaa
     4861 attcatcagt tttgtctggt gcacataagg taaaataagc agacattcct gtctttcccc
     4921 tgctaagtcc tctctcaaca ccttctcata tctactatca acataaacga attgtttctc
     4981 ctcaccctcg atgacacgat aatccatctt tcgataagct acttgtcgtt tctgaaacat
     5041 cttttctaaa taaggaacat gaatatccac ataatcgaaa atacgcacca aagacttatc
     5101 cttgtagttt ctatgaatcc gacctgcata ctgaattaaa ttatttttcc aagaaaaggg
     5161 tgctgccaag ataagcgtgt ccaactgagg taagtcaaaa ccttcgccaa tgtattttcc
     5221 agtagacaac aaaacaaacc ctttatctaa ctgttctaac gtctccagta aactcgttct
     5281 ttctcggact ttggtttttc cgctaataat gtaacagcca tcaacctctt tctctttcaa
     5341 taacttttca aagacatcta tctgttgaat tcgattaact aaaaccaaga tattccgtcc
     5401 ttctgccact tgggctagaa tatccttgag aatcaactga ttcctcgctg agtctgtagc
     5461 aatccaatca ctgagctgta taaaattact tgctttggtc ttttcaatct ccaaatgacc
     5521 aaaagaagtg aatcttaatt gcaattgccg tttaaaatcc gtttccctct tatcagcagt
     5581 atggagtatc tcaccaattc tctgaaaaac aataggctca tgaccattct tacgctcagg
     5641 cgtagccgtc aaaccgtaaa gatacttccc tctaaactga gcaacaactt tttcaaacat
     5701 caaggcagag acatgatgac actcatccac aatcatcatc tcatactcat ccaaaagact
     5761 ttgactattt tccaacttaa atagagattg aatcataacg acatcaacca gtttactcag
     5821 ccatttctta gtcccaccgt actgtccaac atagccgatt accttttcac gacctgatgc
     5881 cgtataacgg atagcctcct cctcttcgaa agtcaaaaag cagtttaagc gatctagcca
     5941 ttggtctaag agttgcttat tatggactag aataattgtt tttgttttcc gttcagagat
     6001 aagagcagca cctaaaacgg tcttcccaaa accagtctcc gcatgaagta aaccattttc
     6061 tttagaagtc atatctgaca gggctaactc ttgctcaaaa gtgagttctc ccttaaattc
     6121 cacgctaata gacctttgta cctttctcct atcttccaca gatacctgct taaatttatc
     6181 ttgcaatgga tacagcaaac ctcttggcaa ccataaataa tgatcggatt ctccaaataa
     6241 atacattctc tcaggaattt gataggttgg ctgtcgcata gcctgcttta aataaaattc
     6301 gggattagaa aatgaagcca tatttttcaa gaaaaacagt gtcttgggtg tcacagacga
     6361 tttttcaagt tggatcatat tggataagac gatcttcaac tccttgtcca attcttgctt
     6421 gcctaactcc tcttggatta acagtgccac tttagcagtt gaaatcttct gaatttcttg
     6481 tagatacctc cattgatctt cataaggctg aaactgttca tccacaaaga ccgttcgccc
     6541 ttgatgataa gcttctcctt gaaaaggaag ggaaattaaa ttatcaaatc cacctttagg
     6601 aagaacatcc tgatttggaa acatgcgatc aaaagaatca aaggacagtt gcatactttc
     6661 ctgcatagcc aattctaata gtttctttcc aaacaggcga gcctttcgac tcagaatcgc
     6721 ttcctcaaag aagaaccaaa tatggagtcc gtaacccgaa cgagaaatct ctaaatgggc
     6781 ttccatctgg cgttccctgg ctattcttcg aatggttaaa ccagcttctt tccaatctcc
     6841 ttcatccaaa tccagtacca aaaaataaca gctatcatct aagtgcatag gaaagatacc
     6901 gatagctgcc tccccacgaa aatgagattt caaaacggag ttcgtcaatg tctggaaact
     6961 ccgtttttca ggtggcaact gcttccaacc ataatcatag gaaggaaaat actgaatttt
     7021 cccttgttca ttgataaaat tcttagcata aacatcataa cggccagcaa acacagaggt
     7081 aaacaattgt aatttttctt gcgtcgtcaa gcgactactt tgaagttgga acatctcctc
     7141 gaactgagag cgttccataa caaactgact cttgtcttta agacgtttat agtggaatga
     7201 aataagatat gaacaaattg attaggaaaa tcaaattaat ttatagaaat cttttagcag
     7261 tcatggcgtc ctattccaga ttcaaaatgc tatacacaag aaaaactcac tatccccatt
     7321 aaaagaagaa aagacatcaa ggacttctac cacaattcca tccaaagaca caaaaacagc
     7381 cataagagtc acctccttga ttcctatagg ctgattataa caagactggc tgaaattgta
     7441 catgaaaata aaatcctaat agtactcatt ttgtatgtga ctaatattcc gtctcgctcc
     7501 agaaggtacg aagtaaatag agtttaaacg agcagtctat aaaattcaaa aaacgcatat
     7561 tatcaagcga cctaaaactt tgggctctat aatatttgta gtgggtaaat tccctatgga
     7621 tattatggag cctattttgg gtagaaaaaa agtcccataa gatctataat gaaaagcgac
     7681 aaaacaactc attagaaaga atcatatgga acaattacat tttatcacaa aattactaga
     7741 cattaaagac cctataaaat tccttacctt gaaacgactg gtatgcctac tagaattctc
     7801 cttagaaaac gtcgttttaa gtgctatcat tgcttgaaaa tgatggtcgc tgaaacttct
     7861 atcgtcaaga aaaatcacca aattcctcgt attatcaacc aaaagattgc gcaaaagtta
     7921 attgaaaaga tttctatgac cgatattgcc catcagctgg ccatttcaac ttcaactgtc
     7981 attcgaaagc tcaatgactt ccactttgag tgtaatttta gaaatctgcc taagattatg
     8041 tcttgggacg ttgaaacagt ccggggagtg actgtttcaa tcgggagatg gagatgagct
     8101 ttattgcgca agattttgaa aagctcgata tcatcactgt tcttgagggt agaacacaag
     8161 ctgtcatccg agatcacttt cttaaatatg atagagccgt ccgatgtcgc gtcaaaatta
     8221 ttactatgga tatgtttagt ccttactatg acttagctaa acagcttcgc tttcaaattt
     8281 ctaggctcag gctgaaacag tctcccagac tgtttcactc ccgaatgcta aaatcgttct
     8341 tgatcgcttt catattgtac aacatcttag ccgtgctatg agtcgtgtgc gtgtccaaat
     8401 catgaatcag tttcatcgaa aatcccatga atacaaggct atcaagcgct actggaaact
     8461 cattcaacag gatagccgta aactcagcga taaacatttt tatcgcccta cttttcgtat
     8521 gcatttaacc aataaagaga ttttagacaa gcttttgagc tattcacaag acttgaaaca
     8581 tcactatcag ctctatcaac tcttgctgtt tcactttcag aataaggaac cggagaaatt
     8641 tttcgaactt atcgaggaca atcttaagca ggttcatcct atttttcaga ctgtctttaa
     8701 aaccttcctc aaagataaag aaaaggttat caacgccctt caactacact attctaatgc
     8761 caaactggaa gcgaccaata atctcatcaa acttatcaag cgcaatgcct ttggttttcg
     8821 aaactttgaa aacttcaaaa aaacggattt ttatcgctct gaacatcaaa aaagaaagga
     8881 cgaaatttgt cctttctcga acttagcttt tcttcaaccc actacaattg acaaagagcc
     8941 aaactttgat gatatacgtt ttcttgtgag aatatttact tcattttttc ctaaaattca
     9001 atgtttactc agtatttgga ttatgaaaaa tcgaggtcta aatctagata cattttttct
     9061 gaagacaaat cattttgacc accgagcaag agattttcaa aaaaagctgt taaaaactca
     9121 ggacgtcgct gtaaaatctt tgcattatct aataccaagg catcacgaaa atacttggaa
     9181 tgttgctgaa atggtatatt atcaatatca aaaccaaact cacgaagata ctgaatcaaa
     9241 aagaccgtta ctgtccgagt gtttccttcg cgaaatggat gaatctgcca gattcctgaa
     9301 ataaaatgct ggatttgttt aaccacatcc gcctgagtta gtgtcgcata tgcaacttgt
     9361 ttttcctgat taaaatcata atctaaggtc atttgaatca tggagtaatc agagtacaca
     9421 acactttcac cattcaaaac aggttcattc tttgtgatat tggtctgacg aaattgcccc
     9481 accggaatag agggttcaaa tatatcttga aacaactcct tatgaatagc aagtaaggtc
     9541 gcaggactaa agctaaagcc tcttcgagac aatagttcta caatacgtag agaaaccaag
     9601 tctgcctctt ccgtacttgc atcaatagta tggtgataag ccgttgcatc ctcataaacc
     9661 tgctcataag tcagttctcc ccgggactgt ttctcagcca aagattccat atacgctgat
     9721 ggcactagat tgtcaacttt ctgcagacca aaacctatcc gccataaatc acgcttcgct
     9781 tcataagaca agtttggatt gtcaatgttg taagttggtt gcatagaaat atcctttcaa
     9841 ttgtttttct tcattaccag taaatcacga aattggttgg aaactggctt aaactataaa
     9901 aagtcctcac aataggttct atgatatata tatatatata tacttcatgt aaataataaa
     9961 agagttataa tcagcactac tgataaattc aataccgatt tcgccatctt aaaacttagt
    10021 caaacgaata ccgttgattt ccagttcatt aagtcgttga cctgtcaaga cttctcgttc
    10081 cttcaactgg aaaagttaag ggggaactaa tacaaatcct ctacgcttct atattcaatg
    10141 atttcatttt tgataatatt ctttttaaac tcaaaatgtt ttaaaggatt atcgattaac
    10201 actaactttg gaatatcatc tagatttgtg accaaggata aaataaaatc cgattgaaac
    10261 tccttgactt tttcaaattc ttttgcagtt aaacgcacac gccccttaga ttttcggata
    10321 ccttttactt cggcaaggta agaatactct tggacatcta cctgaaagtc atatccatca
    10381 ccataaagac gggcatcggt taactgtcca ccttggaatt tttcttcttt atcaaaatga
    10441 aggataaaat aattctcagc ctccattccc gtttcctgta gacgtttgaa tttagttcga
    10501 actattggtt tttcaacgat ggttatgcct gttttcttcc cttcactggc aagtaacatt
    10561 ttaactattt cagcataact atgaacatct tcatttccaa acatcatatc aattaaatct
    10621 ttacgaaaac gataaacttc agctttctgc caccaacctt ttcgattatt gtcaaaataa
    10681 ggatcaaata aatccattct attttttaca actccagttg tttcagcaat tcccagagaa
    10741 accacatagc gataaaactc cgacttgcta aagcattgaa attcttttat aaaaagcttg
    10801 tcaaacttgg caagaccgta gccaattaaa tttaacaatt cataatgagg gtgtttggac
    10861 atcactttct ccatcaatag atattgattt cattatacca aaaaacacgg ctcatcaccg
    10921 tgtttctgtg tttatttcac caacttcttc atctcatcaa tacgtgctac ggtcacatca
    10981 tacttggctt ggtagtcggc ttgtttgtct ttttcttttt ggacaacttc tggtttagcg
    11041 ttggcgacga agcgttcgtt agagagtttc ttaccaacca tgtccagttc tttttgccat
    11101 ttagcgagtt ctttgtcgag acgagctagt tcttcttcaa cattgaggag atcggccagt
    11161 ggcaagaaga tttcggctcc tgtaataacg cttgacatag ccagttcagg tgcagggatg
    11221 gttgatgcga tttccaagtg ttctggattt gtaaagcgtt tgatatagtt gacattgctg
    11281 ttaaagaagg cttccaagtc actatcgctt gtcttaacaa ggatggtgat aggcttgctt
    11341 ggtgctacat ttacttccgc acgcgcattc cgaacagcac gaattaagtc tttgagactt
    11401 tccacaccag tgtgagccgc aaggtcttca aaggctagat taacagttgg gtatgcagct
    11461 gtcacgatag aaccttctga gatttgtcca aagatttcct ctgtcacgaa tggcatgatt
    11521 gggtgaagga gacgaaggat cttgtccaaa gtgtatagta gaacagaacg tgtgatgact
    11581 ttctcttctt cgttatcgct ataaaggact tccttggtca actcaacata ccagttggca
    11641 aattcttccc aaatgaagtt gtaaaggata tgaccagcca caccaaactc gaacttatca
    11701 aagttttcag taacttttgc aatggtttcg ttgagattgt ggagaatcca gcggtccgtc
    11761 acattaccag cctcacctgt tgcaactttt gtgacattgt catgcgccac atccagcgtc
    11821 aaacctccat tgttcatgag gatatagcga gaaatgttcc aaatcttgtt aataaagttc
    11881 catgaagcat ccattttctc ataagagaaa cgcacgtctt gtcctggcgc agaaccgttt
    11941 gaaaggaacc atcgaagggc atcggcaccg tatttctcga taacatccat tgggtcaatc
    12001 ccgttaccga gagacttaga catcttgcgt ccttgctcgt cacgaatgag accgtggata
    12061 aggacgtttt ggaatggttg acggcctgtg aattccaatg actggaagat catacgagac
    12121 acccagaaga agatgatgtc gtaacctgtt accaaggttg aagttgggaa ataacgttta
    12181 aagtcttctg agtcgacttc aggccagccc atggttgaaa atggccagag ggcagaactg
    12241 aaccaagtat ccaagacgtc ttcgtcctga gtccatccgt caccttctgg agcttcttcg
    12301 ccgacataca tttcaccatc agcattgtac caggcaggga tttggtgacc ccaccaaagc
    12361 tgacgagaga taacccagtc gtggacattt tccatccatt gaaggaaggt atcgttgaaa
    12421 cgaggtgggt agaattcgac cttgtcctct gtgtcttggt tggcaatggc gttcttagcc
    12481 aattggtcca tcttgacgaa ccattgagta gacaagcgtg gctcaactac gacacctgtc
    12541 cgttctgagt gaccgactga atggacacgt ttttcgattt tgacaagggc accgatttct
    12601 tccaacttag caacgactgc cttacgagct tcaaaacgat ccatgcctga aaattcaaag
    12661 gcaagctcat tcatagttcc gtcgtcgttc atgacgttga cttgtggcaa gttatgacgt
    12721 tggccaacca agaagtcatt tggatcgtgg gcaggtgtga ttttcacgac accagtacca
    12781 aactcaggat ctgcgtgctc atctccaacg attgggatga gtttattagc gattggaagg
    12841 atgacgtttt taccaatcaa gtccttgtag cgcgggtctt ctggattaac cgcaaccgca
    12901 acgtccccaa acatagtctc aggacgagtt gtagcaactt caagggcgcg tgaaccatct
    12961 tccagcatgt aattcatgtg gtagaaggca ccttctacat ccttgtgaat cacctcaata
    13021 tcagaaaggg ctgtgcgagc tgctgggtcc cagttgatga taaactcacc acgatagatc
    13081 cagcctttct tgtaaaggtc cacaaagacc ttacgaacag cttttgacaa accttcgtca
    13141 agagtgaaac gctcacgaga atagtctaca gaaagcccca tcttgcccca ttgttccttg
    13201 atggtagtgg catattcgtc tttccattcc cagaccttcg tcaagaaaga ctcacgacct
    13261 aggtcataac gcgtaatacc ctcaccacgt aagcgctcct caaccttagc ctgagtcgca
    13321 ataccagcgt ggtccatacc tggaagccaa agggtatcaa agccctgcat gcgtttttga
    13381 cggatgatga tatcctgcaa agtcgtatcc caagcgtgac caaggtgaag tttcccagtt
    13441 acgtttggtg gtggaatcac gattgaataa ggcttagcct tttgatcgcc tgaaggcttg
    13501 aaaacatccg catcaagcca tttttggtaa cgaccagcct caacctcggc tggattgtat
    13561 ttagatgaaa gttctttaga catgtgtgtt ctccttaatt acttttattg acaattttaa
    13621 ccaaaccaat ttttgaaagt tcaatataaa tctcttcaat ttcagcaaat gctgcaagtg
    13681 gtgacatagt ataatttcca ggattatatt taagatcata cttg
//
LOCUS       DAVKLW010000051        13684 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKLW010000051 DAVKLW010000000
VERSION     DAVKLW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.051"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(779..1648)
                     /locus_tag="VW324_001599"
     CDS             complement(779..1648)
                     /locus_tag="VW324_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9727204.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1872..2549)
                     /locus_tag="VW324_001600"
     CDS             complement(1872..2549)
                     /locus_tag="VW324_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9727205.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKI
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFF
                     KKKEDEK"
     gene            complement(2507..3613)
                     /locus_tag="VW324_001601"
     CDS             complement(2507..3613)
                     /locus_tag="VW324_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9727206.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3724..3978)
                     /locus_tag="VW324_001602"
     CDS             complement(3724..3978)
                     /locus_tag="VW324_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727207.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(4318..4764)
                     /locus_tag="VW324_001603"
     CDS             complement(4318..4764)
                     /locus_tag="VW324_001603"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9727208.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            complement(4837..5162)
                     /locus_tag="VW324_001604"
                     /pseudo
     CDS             complement(4837..5162)
                     /locus_tag="VW324_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284991.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5396..7114
                     /locus_tag="VW324_001605"
     CDS             5396..7114
                     /locus_tag="VW324_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9727209.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            7225..7572
                     /locus_tag="VW324_001606"
     CDS             7225..7572
                     /locus_tag="VW324_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9727210.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7850..8686)
                     /locus_tag="VW324_001607"
     CDS             complement(7850..8686)
                     /locus_tag="VW324_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9727211.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8699..9328)
                     /locus_tag="VW324_001608"
     CDS             complement(8699..9328)
                     /locus_tag="VW324_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9727212.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9338..9979)
                     /locus_tag="VW324_001609"
     CDS             complement(9338..9979)
                     /locus_tag="VW324_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9727213.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(10125..11354)
                     /locus_tag="VW324_001610"
     CDS             complement(10125..11354)
                     /locus_tag="VW324_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9727214.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11344..12510)
                     /locus_tag="VW324_001611"
     CDS             complement(11344..12510)
                     /locus_tag="VW324_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9727215.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12654..13667
                     /locus_tag="VW324_001612"
     CDS             12654..13667
                     /locus_tag="VW324_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9727216.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEKGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         4134 a         2932 c         2436 g         4182 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatag cccccctaat
      361 cgcttatttt atagcaaaat gaaatagaaa ctgaacattt tgttgaggaa ggtaaatcaa
      421 ttcctcacaa tgtttgagaa agaagggagg actattccaa cttcaaccca ctataaagca
      481 gacttacaag atagggaacc catctccgat aatatatagt agaatgaaaa ctgcacaatt
      541 tgattggaga attcaaacca atttctcaca atgttttaga aacaagggtc tattatttca
      601 actgcaacct actatagtcc attgaagctg gaatagccca ctgagacttc taaaccactg
      661 ctggagagga gtttgtttag agtttctttc atttccctaa aaaatcaaaa agagagaacc
      721 agtctgatcc tctcttactt atagatggtg ctcactcaaa tgttaaagga gctatttctt
      781 agagataaag gaatttgaat acagctactg ccgcaattgc tgctacgata ggtgctacta
      841 ctggtaccca agaataccac cattttgaat cgcctttatg ctcaccaaga actgattttg
      901 gaaggaaagc atgaaggaga cgtggtccca agtcacgggc tgggttcaag gcaggtcctg
      961 taggtcctcc aagtgatgtt accaaagcca taacgaggaa tccaagtgcc aagtgagcca
     1021 cagaaagtcc tgaagcagtg tgtggagcca cctgtgcttt aatagccaag tcagaaaaat
     1081 caactgtttg tcctgcttct attgcctttt gtttcatgaa ttgaagcact tcagcaccaa
     1141 agaagttttt agtcaaacca agagctgcaa agaacaaaac aaatgaacca acaaactcat
     1201 taatcaaacc attgacagtt gctgcatagc gactttcttt tgtaccatgg tcaatacttg
     1261 aaatagttga gaaagttccc aagatgttat ttgggttttc agttttcaag tagaatggac
     1321 ggtatgttgc cacaactaag gcttggccaa agatagctcc caagacttgc gcgataatat
     1381 aaggtaccac ttgtgcccaa gggaaaagac cgctaactgc aagccctaga gtgaaagcag
     1441 ggttgatgtg attcccagat acgttaccaa acatcaaggc tgggatcata acccccatac
     1501 cataaccaac agcgatgacg atccagccac tttggtgacc tttcgtacct ttaagttcaa
     1561 cgttggcaac tgcaccattc ccaagaatga tcaaaatggc agttcccaaa aattcagtgg
     1621 catacttcag tgcccatgtg aaatccattt gctagattct ccttaaaatt ttttagatat
     1681 tccttatttt atcaattttt accctattta gcaactgatt tctggaaata tttttaaaaa
     1741 aataggattc atagtttccc agttcaacgg acttgtaagc gtttttattc ttcacgaaag
     1801 acctcgttct caaaaccagg aaaaaagccc acatctgtga gcttttcctg gttcctagat
     1861 tctaaaatct attatttttc atcttctttt ttcttgaaga atcctaatcc tagaccagct
     1921 aacaaggtca tgataccagc actagccaag gtagcattag cttctgtacc tgtgtttggc
     1981 aattcctctc tcttacctgt ctcataagtc ggaacttctg ggtctggatt cactggagtt
     2041 tcagtttttg gagtacctgg ttctggagtt ggctctggta ttggagtctc ttgatttggt
     2101 agttctgggt ttggtgtcgg aacacttgga tgctctggat ttggttgtgg gattggtttt
     2161 tctggagtcg gttcacctgg agtacctggt tcctgaggac ttggaatttc tggtttttct
     2221 ggagttggtt tatctggtgt tgataaacgg tcatacctta ccgttatttc tttatcacta
     2281 gagtctgacg taacttcttg tgattcaact gttggaatat ctggatcttt gtacttgtca
     2341 atcttaccag atacaacctc gtcccagttt cctgttgtcc attcaccgta ggttacaact
     2401 cccgtgacct tgttctcaat ttttgtacgg cttaaggtta caggttgaac aacatcttct
     2461 tttacatttt ggttcgtaac tttatcaacg taatgaatga tacgcgttat agtcttcgtc
     2521 tcagtagagg ttgctgtttt gggaaccact gtttcctcaa cattctcacg gtagtaatag
     2581 tcaactgttg caccgtcttc tggtacgcat ttgcaggagt tgctaccaag gtgtatgttg
     2641 ttttccttgt gataactcgg tcttctttgt cctcagttgt tgttttccct tcaatagttt
     2701 ttgattctgt ggtatactca gaacctatcg ctaaatcagc ttttataaca gactctgcca
     2761 acttctcttg gctaccttct ttatagtaat tggctgttac tgtagcagtg gttggcgctt
     2821 cgctttctct ataaactaag gtcactgttc taccttcgct tacaatattc ccagttaaac
     2881 ttgcagaatt tgtatctgct tctttaaaag tataatattt tccgtcagta gtagtcatgc
     2941 tactgagttt tttatctgtg acattgtagc tggtaccaat cagttgtttt ttattggtaa
     3001 tgtaggttcc gtcactttct ttttctccaa ttccagtatc atttttcaat gatagcaaac
     3061 gcccttgttc atcaacatag cgaactttca cattttctga gattagttct gccaattctg
     3121 aggttttttt ctttttcttg atttcttcgg ttattttccc tttctcttct tcgggaatat
     3181 ttagttttgg aatgattttt tcaacaacgg ttcgtgatgg ttccacagta tcttggatga
     3241 ctgaaaagtc agctagaatt gggagattat actgaacacg gtgactttga gtgtttactc
     3301 ctactctttc attattctct gaaaatactt gtacggtata agaaacaaca tcttttccta
     3361 atagaacatc cccagtagag aaatagccgc cttttcctag tttgctatct ccagagccca
     3421 cttctttcct aatcttatca gatagttttt taccagtcag tacattcgtt cgcacaatcc
     3481 ctttgtctac ccctacaaag tgggagaact ttttgaactc ttcagaacca gatctagccc
     3541 aaccattatt aagggcattt gcttttgtat ttgtattctc tctcaaaagt ttggcgatta
     3601 gaattatatt catcggcact tagagttgct gctatatctg actcttgaat accaacttcc
     3661 ttactaccat ttctagcggc agtatatgtg tatttatctg tttctacttc tagctgatta
     3721 tccttaacat gataggtaat tttctcctgg tataagagtc ttcagtagtt tatatactct
     3781 gtcatcatct ttagctttag aagcagctga gtttagccaa gttggggtat taggagtatc
     3841 tttatcagcc tgtttcaaga cattattcaa gtcatttgct agcctattgg ttatcctcac
     3901 tgtgaaagtt ttcttccagt ctgaaccact tttattagac ttagatatgc tcatgaaatc
     3961 ctggtcaaca ttcaacacaa tatcgccttg ttttttagca tcttccgttg cttctgcttc
     4021 aaattgatca tggagttttt gttgctctgg tgtatagttt ttttatttac acgatttaat
     4081 gtactatttt ttattttagt attaagaact ccattaatac gatttttaaa ctctgcttca
     4141 aaaaatccct taatttgttg gtgcgcaagt tcaacattca aacctttctt atacaagtct
     4201 tcattctgaa cgatttcggc tacacggtga atggtttgat tgtataccgt cttctcttaa
     4261 ccagcagtat ccttagtttt aatgactact cgataggttc ctttagaacc gttatagtca
     4321 tctagtttta aaaaagcatt ccaaacattc aaaaatagtt ggaacttccc ttaataaatc
     4381 cgtcactttc tttttcaaga tagcccaagc ttgctcaata ggattcaaat ccggtgaata
     4441 aggtggtaga ggtaagaaaa agtgtttatc ttggatgcag agttcatcca aaatgttctt
     4501 gggatgaaaa ccagcattgc ccatgacaat aacatgaggt gtcttcaaag caggtaggag
     4561 ttgcgttttg aaccactcca caaagaaatc gcttgtcatg cttttcttgt aaatcatggg
     4621 agctataaac tctccgtcta cttgtcctgc aacaattgaa gttcgctcaa aacgacgtcc
     4681 gctaatcttt tcatagactt tctcccctct aggagcccct gcataaggac gatagagata
     4741 gcggtcgatt cctgtttcat caatataaac aggtgctagg tgctttaaac tattaaaatt
     4801 cttaagaaat aaggctactt ttctgggtct tgttccttaa agctcctcat ctttttttaa
     4861 agtgacctta atctgcttta aagctgccca tactgaagga acagcacaat caaaatgtgc
     4921 cgcaatttcc cgtaaaaaag catctggatg agcctctaca aaggctttca attcctctaa
     4981 aggaatcttt cggtttttga cgactcgctt tttcctctct atgtgttctt gttcacgtct
     5041 ttcttttccc acgtgaagag agttctgacg ccaacaccaa aaaacttggc tgcctcgaca
     5101 tggctgtgcc cctctttgat gtaatctaat gctcgctgtt taaaatcagt actatatgcc
     5161 atagctttat tataatatgc ttgtttaaaa ctaagtaact atatcaatgt tttgttatga
     5221 gaagacttac tttttttact gaaattgaat ttttcctagc ctcaacatca tataacccca
     5281 aaattcaatc tgacctggtt tgaagagatt ttcgaagcag ataaggtttt actaaaacta
     5341 aaaaaaatga tataattaaa ctgattaaat gttttaaaaa taaaggagaa tttgaatgtc
     5401 ttaccaagaa aattaccaga aatgggttga ttttgtggag cttcctgact accttcgtca
     5461 agatttggaa aatatggacg aaaaaactaa ggaagatgcc ttctatacaa atcttgaatt
     5521 tggtactgca ggtatgcgtg gcttggttgg tgctggtaca aaccgtatca acatctacgt
     5581 tgttcgccaa gctactgaag gattggctcg tttgattgag tcaaaaggtg gaaacgagaa
     5641 agaacgcggt gtagcaattg cctacgatag ccgtcacttc tcacctgagt ttgcctttga
     5701 atctgcggca gttcttgcta aacacggcat caaatcttac gtatttgaaa gccttcgtcc
     5761 aactccagaa ctatcatttg cagttcgtca tctcaactgt ttcgcaggta tcatggtcac
     5821 agccagccac aaccctgctc catttaacgg ttacaaggtt tacggtgaag acggtggaca
     5881 aatgcctcca cacgatgcgg acgctttgac tacttatatc cgtgcaatcg aaaacccatt
     5941 tgcagttgaa gttgctgatg tggaaactga aaaagcttct ggcttgattg aagttatcgg
     6001 cgaagctgtt gacatagaat accttaaaga ggttaaggac ataaacatca acccagcctt
     6061 gattgaagaa tttggtaaag acatgaagat tgtctacaca ccacttcatg gtactggtga
     6121 aatgttggct cgtcgtgctc ttgcccaagc aggatttgac tctgttcaag ttgttgaagc
     6181 gcaagcaact gctgacccag acttttcaac tgtaacatct ccaaacccag aaagccaagc
     6241 agcctttgcc cttgctgaag aacttggtcg tcaagttggt gcagatgttc ttgtcgcaac
     6301 tgacccagac gctgaccgtg ttggtgttga agttcttcaa aaagatggta gctacctcaa
     6361 cctttcaggt aaccaaatcg gtgctatcat ggctaaatac atcttggaag ctcacaaaaa
     6421 cgctggaact cttcctgaaa atgccgctct ctgcaaatct atcgtttcaa ctgacttggt
     6481 aacgaagatt gctgaaagct acggcgcaac tatgttcaac gtcttgacag gtttcaaatt
     6541 tatcgctgaa aaaatccaag aattcgaaga aaaacacaac cacacttaca tgatgggatt
     6601 tgaagaaagc ttcggttact tgattaaacc atttgtacgt gataaagatg ccatccaagc
     6661 cgttcttgtc gttgctgaac ttgctgccta ctaccgttct cgtggtttga cacttgctga
     6721 cggtatcgaa gaaatctata aagaatatgg ctactacgca gaaaaaacaa tctctgttac
     6781 tctttcaggt gtcgatggtg ctgaacaaat caaagcgatt atggctaaat tccgcaacaa
     6841 tgctccaaca gaatggaacg caacagctat cactgtcgta gaagacttca aggcacaaac
     6901 tgctactgtt gctgacggta ctgttacaaa cttgacaact cctccaagtg atgtgttgaa
     6961 atacacactt gctgacggtt catggattgc cgttcgccct tcaggtacag aaccaaaaat
     7021 caagttctac attgcagttg taggggaaac caacgaagaa tcacaagcta agattgctaa
     7081 catcgaagca gaaatcaatg catttgtaaa ataaagacct ataaaagatg agaccaacca
     7141 acctcatctt ttttcgtatc aaatctaagc aattttagaa actttatggc atactagact
     7201 tatcaatgaa agcgaggtaa tctcatggaa caatttttgg ataacatcaa agaccttgaa
     7261 gtcactacag ttgtgcgtgc gcaagaagct cttgataaaa aagaaactgc aaccttcttt
     7321 atcggtcgca aaacttgccc ttactgccgt aaatttgcag atacattgtc aggtgtcgta
     7381 gctgaaacca aagctcacat ttacttcatc aatagtgaag aaccaagcca actcaatgat
     7441 ttgcaagcat tccgctcacg ctatggaatc ccaactgtac caggttttgt tcatattaca
     7501 gatggacaaa tcaacgtccg ttgcgactct tcaatgtcag cacaagaaat caaagatttc
     7561 gcaggattgt aaaaaatcag ctaacaagtc ttaaaatcac aaaaacgcat aatgtaaggt
     7621 gcaaaaacct tgatattatg cgttttattg ttcctaaaac agttacatca gcatttcatt
     7681 acagaacatt ttaattttgg aagtcaaatc aatttttaac aatgttttag aaatcacagt
     7741 gggctattcc atattcaatt atccatattc aatcgctatt atcgttttcc tagattacaa
     7801 aaaaactacg ttttgctaat caaaacgcag tttcagccac tattttatct tactgtcctt
     7861 cttttacttc tttggttgct acatcttctc caaaccattt ttggctgatt tcttggaact
     7921 tgccgtcctt gtaaagacta gaaaaagctt catttatctt cttaaccaag gttgtatctt
     7981 ccttacgggc tccaaccgca aaagcttctg tttctagtcc aactgtaaag acattataat
     8041 cgtttaaaac accttctgct tctaaataat agtttgcata gacacggtca atcaatagac
     8101 catcaattcg atcgtttttc aaatcaatca aggcttcatt aaaggtttgg tattgattcg
     8161 cttccttatt agcgacaata ttcttcaaaa tttctggatt tgcttcaaag tccgcataac
     8221 cagatgaacc agcttgagct cctaatgtct ttccagtcat atcctttgca gtcgtgatac
     8281 cagatgattt cttcgtaacc aatacctgct cattcttcat atatgagtta ctgaaagcca
     8341 ccttttcacg gcgttcgtct gtagcggaat agccattcca aatcagatca atcgttcctt
     8401 ttgtcaattc agcttctttc aaatcccaat caatcggttg ccaatttacc gtgattccgt
     8461 atttttcaaa aacagctgta gctaaatcaa tatcaaatcc tgcataagaa ccatctttct
     8521 gagcaaatcc cattggaaca aaagtactat caaatccaat agtaatagac ttgttagact
     8581 ggtactttga ccaattatct ccactagttt cgctagaatt tttcccacaa gctactaaga
     8641 acaaagcagt catcagactg actaatacaa gcatccattt tttcatccat ttttcctcct
     8701 atttaggttc tactttcaat aatacatctg cgatattttc agcaaactgc aaatcatggg
     8761 taaccacaat ctgggtcatc ccaagttccc tattttgcaa gattagcttc tccacttcca
     8821 aacgtaattc tggatccagg gcagaagttg gttcatcgta gccaatgatt tctgggtcaa
     8881 tcatcatagc acgcgccaaa gccacccgct gcttttgccc accagatagt gagaaaggat
     8941 aggcctctgc gtgtcctcct agtcctaact gttccaagag ttcactcgcc ttcttctcag
     9001 cctcttcctg cttcattccc atggtcttca caggcgataa agtcaaattt tccagaactg
     9061 atagatgagg aaatagttga aaatcttgga agacaaatcc cagtaggttg cgcttctgca
     9121 attcatccag ctctaaaggt tgtccattat aaaagatttg ccctgaatca atggtttcaa
     9181 gacctgcaag catacgtaaa agagttgtct tacctccacc agaaggtcca acgatagcca
     9241 ggatttgctt ttcaggaata cttagactga aatcagacag gatttgtttg tctccaaaga
     9301 ctttattgat atttcgtaat tctaacatag ctgcccccta tctataataa ctgtactttt
     9361 tctcaacttt tttggaaata attgtcacaa tcccaattaa aatcaagtaa atggctcctg
     9421 ccaagaacat aggaaccaga ctagcatcgc ggttagcagc tgttcgacta gccaagataa
     9481 ggtctgaaat tccgagagca tagaccaaag aagtatcctt gaccaaactc ataacttcat
     9541 taaagacact aggaagaacg atcttggtca cttggggcaa gataatatag cgcactctgt
     9601 caaaagggct aaacttcaag accttggcag cctcatactg tcctcttgga atagtgtcaa
     9661 ttcccccacg gaaaatttct gcaaagtaag ctgcatagtt gagaacaaag gcaataatag
     9721 ctgcaggaag gcggtctaaa cgaatcccaa tacttgggag cacataatag ataaaaatca
     9781 gttgcaagag taagggggtt cctcgcataa cccagatgta aatgttaatc agataatgga
     9841 ggggcttcca atggacttgc aaggcaaagg caatcaaaac gcccaaggga atcgaaaata
     9901 gcaagaccag tgcaaagacc tgtacagtcg tgctcgcacc attcagtaaa ctcggtaata
     9961 tctcaaataa ataagacata ctgcacctcc taaaagtaat tgttcctatt atagcatgaa
    10021 taaacaagat taccaagtat ttttgtaaaa agattctacc atatacaaat aaaaaccgta
    10081 gctatcaaaa gagataaact acagttctat atgtttaaac attcttacaa tctctcaaac
    10141 agttcttgca tttgcacatc atctggaacc agttttaagt aagtgtgagc atggactttt
    10201 gcttcttcaa aatgtcccaa ttcacgcaag agatagatat agtgttccag aaattctgga
    10261 ttgtccttca aatctcctgt caactcttga taatgctcat aagcagtatc caaatcgtcc
    10321 atttcttgat aagaacgagc aatcatccac ttggtcaaaa gattttctgg ctcctcactc
    10381 tgcaagtcta gaatatcctc ataacgctcc tgctccagat aaatagtggc taaacgaagc
    10441 aagatttctt ctgtatcctc agcgtcttct tttgcagtaa ggagataatt ttctgcacca
    10501 ctagcatcat gcaattcata agaaaattgt gaagcagcta gcaagaggcg agtttcaaag
    10561 ggatttttct ctaatccttg cttagcgata cgcagggctt cttgaacttg atgttcctta
    10621 tgtaaagcct gactgtaccc atactcatag ccttcaaagt caggagaaat ggtatcaagc
    10681 tgcttaaagt agagggtggc tttttgatat tcttcttgat caaaataaag actggccaac
    10741 tcaaaagctg ttaagtcatc gtattctaac tccagggctt tttctaaaaa ctcagtagcc
    10801 gtttcaaatt tccctaactg agcataggca aagccaattc gttgataggt ggaaatgccc
    10861 gtttgctcat aaatcgagcg attatctaac tgggcatagg cttgaatagc cgcttggtaa
    10921 ttttccaact cactatccaa ctctgccaaa cccaatatca agagagaatc ctctgagtag
    10981 gtcaaggcct ccaataattt ctcacgtgcc acatctgtca aaccttccag ctggtaaagg
    11041 tctgccttca aaaccaaaga cgagacatac cagtcactgt cagcttggat ttcctcaaga
    11101 taggtaaagg cttcttctat ttgaccatcc tcgctagcaa ttgcagctag attaagatga
    11161 acctctggaa aatcctctac aattttcagg taaatttcct tggcctgagg atagaaacca
    11221 atcccttcaa gataagttgc caactcatac agaagattac ttgaatcatt ttctaaagct
    11281 ttggcgaaat aatgctcagc cttagttaaa tcttgctcct ccaaagcctg taacatctgt
    11341 tgactattgt tcactcttga cttcctcacc ctctaattca tatagaccac tgactacctt
    11401 ataccattcg aaaatggctg aaatgacaac cttagcagag gcataaaccg gaataccaag
    11461 taaaactccc cagataccaa acatagatcc tgaagttaac aaaacaaaga gaacattaat
    11521 agggtggatg tttaattgac ttcccaaaat caatggagag acaaaacggc cttcaatagt
    11581 ttgttctacg ataaagacaa tcactacttt caaaagcatg actggaccag caatcaaacc
    11641 caatactaga gcaggaagca tggctagaaa gctaccaaga taagggacca gatttaaaat
    11701 accagcagta acccccagcg taaccgcata gcgtagacca ataatcttga agaagatgat
    11761 aaacattact gctacaataa tagccactgt cacttgccct cgaacatagt tggacaactg
    11821 ttgattcaca tctgatagaa cttgtccaac aggttccttc aattttcttg gaatgaattg
    11881 ggtcaaatag ttacgcaagc ctttcccatc acgcaagaga taaaagagca tgaaaggaac
    11941 gataatcaag gcaacaatca cttgagaagc tccgctaata aaggcactca cccagttgac
    12001 tgcctgagat gaaaccttac ttgccaaaac tgtagcctgg ctagaaaaat tggtcaaaac
    12061 ttgctctaat tgaggtctga aatcatctgg caggtgctgg gctaccaatc cattaacaat
    12121 cctatctata tcttctaagt aaacaggaac gtttcttgca aaggtcaaaa cctgacgttg
    12181 cagatttgga atggcgactg ccaagcccca aatgataaag agagcgatga taacaaagac
    12241 aatagtgata gctataacac gattaacctt atgcttctcc atccaatcaa caataggatt
    12301 caacaaataa tataacaaac cagacaaaat gactggcaac atcacaactg ctaaaaagtc
    12361 taaaacaggt gaaaatagaa aactaatctt acttaaaata aaaagattca gtcccaataa
    12421 taaggttact aaaaataccg taattgcctt gttatctaaa aaccacttga aaaaccaaga
    12481 taggctaaaa tgtttctctt tttgctccat aaatacttcc tttctattgt tctctcatta
    12541 taccattttt gaaacaaaat aactcttttt taaagtgctt acatctaatt ggatatcttt
    12601 aatttacttg tttttgtgat ataatagtct tatctttaca cagaggtata gtaatgaaag
    12661 aaactgttta ttttggaact tatacacgtc gtacttctca agggatttac aaggcagact
    12721 ttgatacaga aactggtcag ctttcaaatc tagaactttt tgcagctgag ccaagtccaa
    12781 cctaccttgc ctttgaccag caccaacatt tatacactgt tggtagccaa gacgataagg
    12841 ggggaattgc agcctatcaa actgacggga ctgtgttaaa tcatgttgtt gaaaaaggag
    12901 ctccccactg ttatgttgct gtcgatgaaa agcgtgattt ggtttacgca gccaactatc
    12961 acaagggaca agtccttgtt tataaacgcc aggaagatgg tagtcttcta cttagtgata
    13021 tggatcaaca cagtggccaa ggtccacatg aaaatcaagc ttccccccat gttcactata
    13081 cagatttaac acctgaccac tatctagtga cctgcgactt gggaactgac caagtcatca
    13141 cctatgacct cgatcaagaa ggaaaattat ctaagctcta tacctatcac agcaagccag
    13201 gagcaggctc acgccatatc attttccata accactataa aatcgcttat ctcatttgtg
    13261 aactcaatag tactatcgaa gttttaatct acgatggcgt tggcgaattt gaacgtatgc
    13321 aggtcatttc aactttaccg gaagcttacg aaggctttaa tggaaccgct gctattcatc
    13381 tctctaaaga cggtaaatac ctctacgctt ctaaccgtgg ccatgattct atcgcagtat
    13441 ataccatcct tgcggacggt agcttagagt tattagaaat cgttccaacg catggtcaga
    13501 ctccacgtga ttttgatttg acacccgacc aaaaatttct cattgttgtc catcaagact
    13561 ctgacaatgc aactgtcttt aaacgtaatt gtgacaatgg tcgtctagca gaactctcca
    13621 acgacttcca tgttcccgaa gcagtctgca tccgttttgc tccttaaaac accataaaaa
    13681 atgg
//
LOCUS       DAVKLW010000052        13660 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKLW010000052 DAVKLW010000000
VERSION     DAVKLW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.052"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..625
                     /locus_tag="VW324_001613"
                     /pseudo
     CDS             1..625
                     /locus_tag="VW324_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(832..3090)
                     /locus_tag="VW324_001614"
     CDS             complement(832..3090)
                     /locus_tag="VW324_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9727217.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(3319..3549)
                     /locus_tag="VW324_001615"
     CDS             complement(3319..3549)
                     /locus_tag="VW324_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9727218.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3739..4422
                     /locus_tag="VW324_001616"
     CDS             3739..4422
                     /locus_tag="VW324_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9727219.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4422..5156
                     /locus_tag="VW324_001617"
     CDS             4422..5156
                     /locus_tag="VW324_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9727220.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            5310..6167
                     /locus_tag="VW324_001618"
     CDS             5310..6167
                     /locus_tag="VW324_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9727221.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            6304..7125
                     /locus_tag="VW324_001619"
     CDS             6304..7125
                     /locus_tag="VW324_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEU9727222.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            7139..8011
                     /locus_tag="VW324_001620"
     CDS             7139..8011
                     /locus_tag="VW324_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9727223.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            8293..8976
                     /gene="rpiA"
                     /locus_tag="VW324_001621"
     CDS             8293..8976
                     /gene="rpiA"
                     /locus_tag="VW324_001621"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9727224.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            8990..10201
                     /locus_tag="VW324_001622"
     CDS             8990..10201
                     /locus_tag="VW324_001622"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9727225.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            10203..10745
                     /locus_tag="VW324_001623"
     CDS             10203..10745
                     /locus_tag="VW324_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9727226.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            10761..11570
                     /locus_tag="VW324_001624"
     CDS             10761..11570
                     /locus_tag="VW324_001624"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9727227.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRTYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            12046..13026
                     /locus_tag="VW324_001625"
     CDS             12046..13026
                     /locus_tag="VW324_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9727228.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAEVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNDRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRSSSQPSAEPMENIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHT
                     NFQSKPWWQVDLAKEETIRQINIYNRTDTAQDRLANFDVILLDSSGKELE"
     gene            13051..13563
                     /locus_tag="VW324_001626"
     CDS             13051..13563
                     /locus_tag="VW324_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9727229.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         4075 a         2456 c         3052 g         4077 t
ORIGIN      
        1 gcttatttga ctgaaatagc ttctgaattt ggctgtcatc taactaccat ccactatgcg
       61 ctcaaagcta tgggttacac tcgaaaaaaa gaaccacacc tactatgaac aagacccaga
      121 aaaagtagcc ttatttctta agaattttaa tagtttaaag cacctagcac ctgtttagat
      181 tgacgaaaca ggattcgata cttattttta tcgagaatat ggtcgctcat taaaaggtca
      241 gttaataaga ggcaaagtat ctggaagaag atatcagaga atttctttgg ttgcaggtct
      301 aacaaatggt gagttaatcg ctccaatgac ttacgaagag acgatgacga gcgacttttt
      361 tgaagcttgg tttcagaagt ttctcttacc aacattaacc acaccatcgg ttattattat
      421 ggataatgca agattccata gaatgggtaa gttagaactc ttgtgtgaag agtttgggta
      481 taacttttta cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc
      541 tcatatcaaa aagcacctca aaaaggtatt accaagttgc aatacctttt atgaggctct
      601 tttgtcttgt tcttgtttca attgactata gcagattgaa ataagatgag aacaaatcga
      661 ttgggaaagt aaaattaatt tctataaatg ttttagcaat tgtttcgtac tatttcagat
      721 tcagtctact atatatcata atggacaaag ggcaagctgt caagaaatta cagttatttt
      781 agtaaaaaaa gaagcagaaa aatttcattt tctactcctt ttctatagga tttatttttc
      841 tttttcagaa cttttaactt cagctttttt ggcagattta atctgaatgt ttcccttgct
      901 agtcataact gctttgagat ctttttcgct tggattttca aggtagtagt cagtgattgt
      961 gtcctcaata tagtcttgaa tagtccgacg aagtgggcgt gctcccattt ttggatcata
     1021 acctaggtca accaactttt ccttgacctt gtcagttaca tccaaacgaa tgttgttgct
     1081 agagaggcgc ttgttaacat ctgctagcat gagctcgaca atctgaagga ggttatcctt
     1141 gctgagagcc ttaaattcga taatgccatc aaaacggttc ataaactctg ggctaaagaa
     1201 gttaccgagt tcaccgagga cagaattggt acgtccttct ctagcagcac caaatccaac
     1261 gctagcttcg gtctttcctg tacctgcatt tgaggtcatg ataatgatgg catccttgaa
     1321 gctaacggtg cgtccttgcc cgtctgtcaa acgaccatcg tccaagactt gaagaaacat
     1381 gtgcataaca tctgggtgag ctttttccac ttcatcgaga aggatgagag aatacggatt
     1441 gtggcgaact ttttcagtta attgaccagc ctcatcatag ccaacataac ctggaggagc
     1501 gccgaccaac ttagccacac tatgtttttc catgtattca ctcatatcaa agcgaatcat
     1561 actatcagca gaaccaaaaa gttcgatagc cagttgtttg gaaagttctg tcttaccgac
     1621 accagttggt ccaacgaaga ggaagcttcc gattgggcgg ttaggggtac caagtccgac
     1681 acgattacgg cgaatagcct tggcaatctt atcgactgca tcatcttggc caataacatg
     1741 agacttgaga tcttcggcta gatggatgag ttgagattgt tctttctctt tcaaatcacc
     1801 aacagggata ttggttttct gctcgataat gtgctcaata gttttctcgc tgatgatagg
     1861 agtatcctgg tctgtgatct ttttcttttg catttcctta tacttggcaa tctggtcgcg
     1921 gaagtaggcc gccttctcaa aatcttcttc tcgtgtagct tgagacttga gattttcagc
     1981 ctcaatcaag cgctgatcaa ttactttagg atccacaaaa ttcaaggtca agttcatctt
     2041 agaaccagct tcatctagga ggtcaatggc cttgtcaggc aagaagcgat cttggatgta
     2101 gcgattggaa agagttgcag ctgcttcaat cgcagcatcg gtatattgaa cgtggtggta
     2161 atcttcgtat ttcttttgaa tccctttgag aatagtgatt gtttcatcca ccgttggttc
     2221 atcgacttta acaggctgca tacgacgctc gagggcagca tccttttcaa tgatacggta
     2281 ttcattgagg gtagtagcac cgactagttg cagttctcca cgagcaaggg ctggcttgag
     2341 gatatttcct gcgtccatat taccatcact cgcagaacca gcaccaacaa tttcatggat
     2401 ttcatcgata aagaggatga tgtcttcacg tttgcgaatt tcttccatga gtttttgcat
     2461 gcgttcttca aattgtcctc gaatccccgt tccttgaact aagctaacca catccagacg
     2521 gatgacttgt ttaccttgga gtttatgtgg cacatcgcca tcgacaattt tctgagctag
     2581 accttcgaca acggccgttt ttccgacacc aggttcaccg ataaggacag gattattctt
     2641 ggttctacga ttgagaatct cgatgacacg gataatctca tcgtcgcgcc caataacggg
     2701 gtcaatgtct ccacgacggg caatttcagt tacattaata ccaaattctt ccagcaggcc
     2761 tttttcttgg ctaggtggcg gagtttgagc agatccacga ttttgggaac cataaccgcc
     2821 gtttccaccg taacctccac ctgattgggt tgggggaata ggaggagtat tgctagaagg
     2881 tctgaaattg tttagatcat tgaagaaatc accaaaggga tcgaagtcac gattgttcag
     2941 atccgtcata cctttgaaga ggctattgtt aggatctgtt ttgataatct tatagcagtt
     3001 ttgacagagg tcaatttgtt tttgttttcc attgagattg gtgtaaagat gaattgttga
     3061 gtcgttaatt ttacagtttt gacaaagcat acatctacct catttctttc tttagcctga
     3121 cttgtttaaa ggtcaaaaat agtcaaattc tatactctca ttataacagg attggggtgt
     3181 aaagcaagta aaaagattga agctttgaga agttgttaca atgaaaattt ttgtgaattt
     3241 ggactataaa aaaatcctat ttgtgagaca aaaaggattt tacttagaca tgcagggaca
     3301 atcccaactt gttttagatt agtagagaag ttcgtaaatt tccattgcga ttaagtcaat
     3361 gctatcaaac gtatacgttt cgcgatcttc tacgtcacga agggtaaaga ttgggccgtt
     3421 ttcttcgtcg tggctgtacg caacttctgc aacaacaact ccttcgcgtt caaaattacg
     3481 ttttaaattt ccgccatcgt ttgccattgc ttcaaggcgg ttgatgattc taaccaaatg
     3541 tgattccatt ttgattctcc ttcatttctt atcgttacta ttttaccata aaggagggca
     3601 acttgactag aaaaaactaa gatttctcgc taattctgct agtttaaaga tttttaaata
     3661 aattggataa aaaatttaaa ttttaattca aaacatgtta taatcataca gtattctatt
     3721 ttagaaagca gtgtgactat gaatttttct tttttaccta agtatttacc ttattttaac
     3781 tatggggctg ttgtgacgat tcttatttct atctgtgtta tctttttggg aactattttg
     3841 ggtgttgtct tggcttttgg gcaacgttca aagtttaaac cgcttgtttg gttggccaac
     3901 ttgtacgttt ggattttccg tgggacaccg atgatggttc aaattatgat tgcctttgct
     3961 cttatgcata tcaatgctcc gactattcag attggaattt taggtgttga tttttcgcgt
     4021 ctgattccag ggattttgat tatctctatg aatagtggtg cttatgtttc ggagactgtt
     4081 cgtgccggaa tcaatgcggt tccaaaaggt cagctagagg cggcttattc gctagggatt
     4141 cgtcttaaaa atgcgatgcg ttatgtgatt ttgccacaag cagtcaaaaa tatcttgcca
     4201 gcattgggga acgaatttat caccattatc aaggacagct ccctcttatc agctattggg
     4261 gtcatggagt tgtggaatgg ggctacaaca gtttctacaa caacctatct acctttaaca
     4321 ccacttttat ttgcagcatt ttactacttg attatgacct ctattctgac agtagccttg
     4381 aaagcttttg aaaaacatat gggacaagga gataagaaat aatgacagaa accttgataa
     4441 aaattgaaaa tttacataaa tcttttggaa agaatgaagt attgaagggc atcaacctcg
     4501 agattaaaag aggagaagtt gtcgttatca tcggtccttc agggagtggg aaatctacct
     4561 tgcttcgctc tatgaatttg ttggaggaag caaccaaggg gaagcttatc tttgagggag
     4621 tcgatattac ggacaagaag aatgacctgt ttgccatgcg tgagaagatg ggcatggttt
     4681 tccaacaatt taacctcttt cctaatatga ctgtgatgga aaatattacc ttgtcaccta
     4741 tcaagactaa aggtgaaagc agggaagttg ctgagaagag agcccaagag cttttggaaa
     4801 aagttggttt gccagataag gcagacgctt atccacagag tctgtcgggt gggcagcaac
     4861 aacggattgc catcgcgcgt gggttggcta tggaaccaga tgttttgctc tttgacgaac
     4921 caacttcagc cttggatcct gaaatggtag gtgaggtatt ggctgttatg caagaccttg
     4981 ccaagtcagg gatgactatg gttatcgtaa cacatgagat gggatttgcc cgtgaggtgg
     5041 cagatcgtgt tatctttatg gcagacggtg tggttgttga agatgggaca cctgagcaga
     5101 tttttgaaca aacccaagaa caacggacta aagatttctt gagtaaggtt ttataatcta
     5161 ttttaaaatt tcaagacaag attagtgaaa agttcaacca gagctttttc ttatagtttg
     5221 aaactatagg ataacctagg aaagaagtgt tagagggcac tagcagtttt tggtataatg
     5281 gaagatattt gaaagaaaag agaagtgata tgacacagat tattgatggg aaagctttag
     5341 cggccaaatt gcaggggcag ttggctgaaa agactgcaaa attaaaggaa gaaacagctc
     5401 tagtgcctgg tttggtagtg attttggttg gggataatcc agccagccaa gtctacgttc
     5461 gcaacaagga gaggtcagct cttgcggctg gttcccgtag cgaagtagtg cgagttccag
     5521 agaccattac tcaagaggaa ttgttagacc tgattgctaa atacaatcag gatccagctt
     5581 ggcatgggat tttggttcag ttgccattac caaaacacat tgatgaagag gcggttctat
     5641 tggctattga cccagaaaag gatgtggatg gtttccatcc tctaaatatg ggacgccttt
     5701 ggtctggaca tccagtcatg attccatcaa cacctgcagg aattatggaa atgtttcatg
     5761 aatatgggat tgaattagaa ggtaaaaatg cggttgtcat cggtcgttct aatatcgttg
     5821 gaaaacccat ggctcagctt cttttggcca agaatgcaac agtgactttg acccactcac
     5881 gcacccataa tcttgctaag gtggctgcta aagcagatat tctggtggtc gcaatcggtc
     5941 gtgccaagtt tgtgactgct gactttgtca aaccaggtgc ggtagtcatt gacgttggga
     6001 tgaatcgtga tgaaaatggt aagctctgtg gggatgtgga ttatgaggca gttgccccac
     6061 ttgctagcta tattacgcca gtccctggag gtgtcggccc tatgaccatt actatgctga
     6121 tggagcaaac ttatcaagca gcacttcgga cattggatag aaaataagag aaaaatcgct
     6181 gaagagagtg tattttctat agctatattt aaaatggcag aaatgaatat taaattttag
     6241 atataagatt acaaaaggag gtctgcgcct cctttttgtt gtataatgga gtgaggtgat
     6301 tagatgattt taaaaattta taatagggaa tatagtttac aatgggatgg aatatactac
     6361 ctagcactaa ttgattatcc aaatattcaa gagtgggaat tagaaaaaat tgctaaattt
     6421 atagcttacg aaaaacttca taaacgtcaa acaagtattg agtgtgctga ttcttgttta
     6481 aaaaaagaaa ttttagatta catctgtcag catccctttc tgccaccatt tacccctaca
     6541 gataaaagag tagcctcgac ttatgatcta cataagaggt tagtgacttc agactactgt
     6601 agtcatacta cgactataga tgcagcgatt tctatcttta aaacaggtca gctcttatct
     6661 gctgtgaaag cctttaggcg agatgctgaa gagttggttt tggatagtag aaatgctgca
     6721 tcggatccga tagattattt tgattatgtc atgttagggt ggtcaaatac aagttctggt
     6781 tatcgattag cgatggagcg tttattaggt cgagctcctt cagagaaaga attacaagac
     6841 aagtttattc ctggagtgag ttttcatttt atctatacag atttgattaa agttactggt
     6901 tatatttttg atggctacca tgttgcaaaa attaaggaca tgttgaattt atttagtgag
     6961 ttgtatattt gtattattcc aactcataat aagagccaat ttgaaaatat tattccaact
     7021 aaaatacaag atagggtgca ttatcttgac tatgctggag aagacttaga agagtggact
     7081 aagaaagtct atcaagttgt tttaaaacaa tcagataaag gatagttgag gaagaacgat
     7141 gaaagtgatt aatcaaacct tattagaaaa agttattatt gaacgttctc gttcaagtca
     7201 taaaggagat tatggtcgtc tgctgctgct tggtggtact tatccttatg gaggtgccat
     7261 catcatggct gctttagcag ctgtaaaaag cggtgcagga ttggtgaccg ttggaacgga
     7321 cagggaaaat atcccggctc tgcacagcca tttacctgag cctatggcct tttctcttca
     7381 agaccagcaa ttgttaaaag agcaattgga gaaggcagaa gttgtcttgc tggggcctgg
     7441 tttacgagac gatgcttctg gagaaaatct agtaaaacag gtctttgtta atttaagcca
     7501 aaatcagatt ttgattgtag acggaggtgc cttgaccatt cttgctagga caagtttgtc
     7561 atttccctca agccagctta tcctaactcc tcaccaaaaa gaatgggaaa aattatctgg
     7621 aattacgatt gaaaagcaaa aggaagatgc aacggctagt gttctgactt ccttccctca
     7681 aggaacaatt ttggtggaga aaggcccagc tactcgtatt tgggaagttg gccaatctga
     7741 ttattaccag ttacaggttg gtggtcctta tcaggcgact gggggaatgg gagatactct
     7801 ggctgggatg atcgcaggat ttgtaggcca attccgacag gccagtctct acgagcgtgt
     7861 ggcagtagcg acccatcttc attcagccat agcccaagaa ctatctcaag aaaattatgt
     7921 ggtcttaccg acggaaatca gcaggtatct tcccaaaata atgaaaatta tttgtcaaca
     7981 agagagggta agcaaagata aacttgttta aaatagacag ataaatctac ttccttctct
     8041 taatttatga ttcttgaaat gcgcctgctt taaggctaaa ctagacgtat caaagttaag
     8101 agtttgtttt tgtaactgga aagagatgaa caaatcaaat acataaaaga taaactcatt
     8161 tttattcgtt tggtaaaaat aaaatgcata tttttaaaga aagattgatg aaaaatgtag
     8221 atttgatgtt tttacttgaa taaaaagcgg tttcatagta aaataagaac tgagaatgat
     8281 ggaggtaaat cggtggaaaa tctgaagaaa atggcaggta tcaaggctgc tgagttcgtg
     8341 agcgatggaa tggtcgttgg acttggaaca ggctcgactg cctattattt tgtcgaagaa
     8401 atcggtcgtc gtatcaagga agaaggattg cagattacag ctgtaacaac ttctagtgtg
     8461 accactaaac aggcagaagg ccttaatatc ccgctcaagt ctattgacca agtagatttt
     8521 gtcgatgtga cggttgatgg cgcggatgaa gtggatagtc agtttaatgg aatcaaaggc
     8581 ggtggtggtg cccttctcat ggaaaaggtg gtcgcaacac catcaaaaga atacatttgg
     8641 gtggtggatg aaagcaagct ggtcgaaaaa ctaggtgctt ttaaattgcc agtagaagtg
     8701 gttcagtatg gtgcagagca ggtctttcgt cattttgaac gagctggcta caaaccaagt
     8761 ttccgtgaaa aagacggcca acgttttgtg acggatatgc agaattttat cattgacctc
     8821 gccttggatg tcattgaaaa tccaattgct tttggacaag aattggacca tgtcgttggt
     8881 gttgtggagc atggtttatt caaccaaatg gtggataagg taatcgttgc tggacgagat
     8941 ggagttcaga tttcaacttc aaaaaaagga aaatagaagg gggcataaga tgtctaaatt
     9001 taatcgtatt catttggtgg tactggattc tgtaggaatc ggtgcagcac cagatgctaa
     9061 taactttgtc aatgcagggg ttccagatgg agcttctgac acactgggac acatttcaaa
     9121 aacagttggt ttgaatgtcc caaacatggc taaaataggt cttggaaata ttcctcgtga
     9181 aactcctctt aagactgtag cagctgaaag caatccaact ggatatgcaa caaaattaga
     9241 ggaagtatct cttggtaagg atactatgac tggacactgg gaaatcatgg gactcaacat
     9301 tactgagcct ttcgatactt tctggaacgg attcccagaa gaaatcctga caaaaatcga
     9361 agaattctca ggacgcaagg ttattcgtga agccaacaaa ccttattcag gaacggctgt
     9421 tatcgatgat tttggaccac gtcagatgga aactggagag ttgattatct atacttcagc
     9481 tgaccctgtt ttgcagattg ctgcccacga agacattatt cctttggatg aattgtaccg
     9541 tatctgtgaa tacgctcgtt cgattaccct tgagcgtcct gcccttcttg gtcgcatcat
     9601 tgctcgccct tatgtaggtg aaccaggtaa cttcactcgt acggcaaacc gtcgtgactt
     9661 ggctgtatct ccattttccc caactgtttt ggataaattg aatgaggctg gtatcgatac
     9721 ttatgctgtg ggtaaaatca acgatatctt taacggtgct ggtatcaacc atgacatggg
     9781 tcacaacaag tcaaatagtc atggaattga tacactattg aagactatgg gacttgctga
     9841 gtttgaaaaa ggattctcat tcacaaacct agttgacttt gatgcccttt acggccatcg
     9901 tcgtaatgct cacggttacc gtgattgctt gcatgagttt gatgaacgct tacctgaaat
     9961 tatcgcagct atgagagaga atgaccttct cttgattact gcggaccatg gaaatgaccc
    10021 aacgtatgca ggaacggatc acactcggga atatattcca ttgttggcct atagccctgc
    10081 ctttaaagga aatggtctca ttccagtagg acattttgca gatatttcag cgactgttgc
    10141 cgataacttt ggtgtggaaa ctgctatgat tggggaaagt ttcttagata aattggtata
    10201 agatgacgcg ctatgctttg ctggtgagag gtatcaatgt tggtggtaag aataaggtcg
    10261 tcatggcgga gcttcgtcaa gaattgacaa acttgggact ggaaaaggtt gagagctaca
    10321 tcaatagtgg caatattttc tttacttcga tagattccaa agcccaattg gttgaaaagc
    10381 tagagacttt ctttgcagtc cattatccat ttattcagag cttttcttta ctgagtctag
    10441 aggactttga ggcggaactt gaaaatctac cagcttggtg gagcagagac ttggcacgaa
    10501 aagattttct cttttacact gagggtttgg atgtggacca agtcatcgcg acagttgaaa
    10561 gtttagagct gaaagatgaa gtgctttatt ttggaaaact tgggattttc tgggggaaat
    10621 tttctgaaga atcctattct aagactgcct atcataagta cttgctgaag gtgcctttct
    10681 accgccacat tactattcgt aatgctaaaa cctttgacaa aattggtcaa atgctaaaaa
    10741 aataataaag gagacacaca atgacatttt taaacaaaat ccatgaaact gctactttcc
    10801 tgaaagaaaa gggaattgca gcccctgagt tcggtctaat ccttggatca ggacttggag
    10861 aattggcaga agaaatcgaa aatccagttg tagtagacta tgctgagatt ccaaactggg
    10921 gccgttcaac agtagtcggt catgctggta aattggtata tggtgaactg gcaggtcgca
    10981 aggtcttggc tcttcaaggt cgtttccatt tctatgaagg gaatcctctg gaagtggtga
    11041 ctttcccagt tcgtgtgatg aaagttcttg gatgtgaagg tgttattgta accaatgcag
    11101 ctggcggtat cggatttggt cctggtacct tgatggctat ctcagaccat atcaacatga
    11161 cggggcaaaa tccattgatg ggtgaaaact tggatgactt tggcccacgt ttcccagata
    11221 tgtctaggac ctacacacca gaataccgtg ccactgccca tgaagtggct aaaaaactta
    11281 atatcaagct tgatgaaggt gtctatatcg gagttactgg tccgacttat gaaacaccag
    11341 cagaaattcg ttcctataag acactgggag cagatgcagt tggtatgtct acggttcctg
    11401 aagttatcgt ggcagcccac tctggcttga aagttctggg aatttcatgt atcactaact
    11461 ttgcggctgg tttccaagaa gaactcaatc acgaagaagt tgtagaagtg actgaacgtg
    11521 ttaaaggtga tttcaaaggc ttgcttaaag cgattcttgc tgaattgtaa gaaaaaagat
    11581 ttaaaagggg gagtgcctct gtcttttcag gattgactgc ctatccggat taaagaagaa
    11641 acagaggaat actatgagct tcttcctgct cttataactg aaagaagcgg aagaataggt
    11701 atgtctgatc tgatagccag cattgtgaaa gacaagattc taggatacta gcattagctt
    11761 cctagccaag tagactagta tgataaggag agatgagaat gaattgactt tctgaatttc
    11821 tcagtcttat catatatagc acaatgagat ttcgcttgag tctgcttgta aataaacgaa
    11881 aagaaagata agaaataatg aaaattggtc aacgaattat gcgctttggc ataaaaaatt
    11941 aagtatcgga gttgtatctg ttgtagtcgg ctttgatttc tagctccagc tggaatttca
    12001 gccaatgaag taaagcaaga tgtaacatct gaagtggtaa taggtgtgct agattctaag
    12061 gaggaattga aagagtcaga aaatgatgct ccaaaactag aaactcctct tagagaggag
    12121 ccaagactag ctcctcaaac gcttccggaa gcaagtgaag ttcttgaaaa caaaagggaa
    12181 gagtcaaaag tagagataac agagccagct caagcggatg atatccgcaa ggttgttggg
    12241 gaattagcca aggatataag tattactaag ttgtatatga caggtcattc tcttggatgc
    12301 tacctagctc agattgcaga ggttgaagct taccaaaaat atcctgattt ttataaccat
    12361 gtattgagga aagtgacaac tttcagtgct cctaaagtga ttacttccag aactgtttgg
    12421 aatgctaaga atggtttctg ggatgttggt ttggaaagtc gtaaattagc tgttagtgga
    12481 aaaattaagc attatgtggt tgataatgac aatgttgtga ctcccttgat tcataatgat
    12541 cgtgatattg ttacatttac aggtaattca cgctttaaac accgttctcg tggctatttt
    12601 gaaagtccaa tgaatgatat tcctaacttt aatattggta aacaagctac cttggataaa
    12661 catggttatc gtgatccgaa attggataaa gtgcgattct ttaagaaaca ggctctgcct
    12721 cgatcttcta gtcaaccaag cgctgaacca atggaaaata ttgccttagg aaaacaggtt
    12781 actcaaagtt cgacagcttt cggaggagat gctagaagag ctgtggatgg caaagtcgat
    12841 ggtaactatg gtcacaattc tgtcactcat acaaacttcc aatctaagcc ttggtggcaa
    12901 gtagatttgg ctaaagaaga aaccattcgc caaatcaata tttacaaccg aacagacact
    12961 gcccaggata gattggcaaa ctttgatgtc attcttttag acagttctgg taaagaactt
    13021 gagtgaaaac gtataacatc tcctaaagat gtgtcagcac aaattacgat taaccataaa
    13081 aaagcgcgct atgttcggat tgagctagaa ggctataatg ccctcagtct tgcagaagtt
    13141 gaagttttcc gctttatagc tacgaatggt gaaacggcga tacaagtttc taagtcagtt
    13201 caaccaatca gtcagactcc tgtgaaggat aaaacattga caattcaaca cagtggagct
    13261 tacattgccc gctactccat aacttgggaa gaagttccag tagataaaga tggaaaccaa
    13321 gttgttcgta gtcattcttg ggaaggaaac ggtcgcaacc agactgcagg ttttgtcctc
    13381 aacctcccaa tcaaagaaaa tatgagaaat ctgcgagtta agattgagaa aaagacgggc
    13441 ctactatgga atagatggca aacaatctat gaaaacagac caattttagc tcaaccccac
    13501 cgtaaaatta cccattgggg tacgacattg aattccaagg tgagtgacga tgatgtcttg
    13561 taatctgatg gtacaatgat agttagtttg tctagtttat aagaaagtac tacctgagct
    13621 tgaataggac tcaggtagct ctctgtgaaa gaacaaaatt
//
LOCUS       DAVKLW010000053        13637 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKLW010000053 DAVKLW010000000
VERSION     DAVKLW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.053"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(380..1573)
                     /locus_tag="VW324_001627"
     CDS             complement(380..1573)
                     /locus_tag="VW324_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9727230.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1711..3153)
                     /gene="gtfA"
                     /locus_tag="VW324_001628"
     CDS             complement(1711..3153)
                     /gene="gtfA"
                     /locus_tag="VW324_001628"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9727231.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(3430..3510)
                     /locus_tag="VW324_001629"
                     /pseudo
     CDS             complement(3430..3510)
                     /locus_tag="VW324_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3511..4344)
                     /locus_tag="VW324_001630"
     CDS             complement(3511..4344)
                     /locus_tag="VW324_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9727232.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4368..5234)
                     /locus_tag="VW324_001631"
     CDS             complement(4368..5234)
                     /locus_tag="VW324_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9727233.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELLYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5248..6506)
                     /locus_tag="VW324_001632"
                     /pseudo
     CDS             complement(5248..6506)
                     /locus_tag="VW324_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(6598..8760)
                     /locus_tag="VW324_001633"
     CDS             complement(6598..8760)
                     /locus_tag="VW324_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9727234.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGV
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            8868..9728
                     /locus_tag="VW324_001634"
     CDS             8868..9728
                     /locus_tag="VW324_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9727235.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKDKIVYLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            9725..10660
                     /locus_tag="VW324_001635"
     CDS             9725..10660
                     /locus_tag="VW324_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9727236.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            10806..12107
                     /locus_tag="VW324_001636"
                     /pseudo
     CDS             10806..12107
                     /locus_tag="VW324_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12211..12298)
                     /locus_tag="VW324_001637"
     tRNA            complement(12211..12298)
                     /locus_tag="VW324_001637"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12262..12264),aa:Ser,seq:gct)
     gene            complement(12305..12378)
                     /locus_tag="VW324_001638"
     tRNA            complement(12305..12378)
                     /locus_tag="VW324_001638"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12342..12344),aa:Ile,seq:gat)
     gene            complement(12414..12484)
                     /locus_tag="VW324_001639"
     tRNA            complement(12414..12484)
                     /locus_tag="VW324_001639"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12450..12452),aa:Gly,seq:tcc)
     gene            complement(12504..12576)
                     /locus_tag="VW324_001640"
     tRNA            complement(12504..12576)
                     /locus_tag="VW324_001640"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12541..12543),aa:Phe,seq:gaa)
     gene            complement(12580..12653)
                     /locus_tag="VW324_001641"
     tRNA            complement(12580..12653)
                     /locus_tag="VW324_001641"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12617..12619),aa:Met,seq:cat)
     gene            complement(12664..12753)
                     /locus_tag="VW324_001642"
     tRNA            complement(12664..12753)
                     /locus_tag="VW324_001642"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12715..12717),aa:Ser,seq:tga)
     gene            complement(12764..12837)
                     /locus_tag="VW324_001643"
     tRNA            complement(12764..12837)
                     /locus_tag="VW324_001643"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12801..12803),aa:Ile,seq:cat)
     gene            complement(12851..12924)
                     /locus_tag="VW324_001644"
     tRNA            complement(12851..12924)
                     /locus_tag="VW324_001644"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12888..12890),aa:Met,seq:cat)
     gene            complement(12930..13003)
                     /locus_tag="VW324_001645"
     tRNA            complement(12930..13003)
                     /locus_tag="VW324_001645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12967..12969),aa:Pro,seq:tgg)
     gene            complement(13019..13092)
                     /locus_tag="VW324_001646"
     tRNA            complement(13019..13092)
                     /locus_tag="VW324_001646"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13056..13058),aa:Arg,seq:acg)
     gene            complement(13102..13187)
                     /locus_tag="VW324_001647"
     tRNA            complement(13102..13187)
                     /locus_tag="VW324_001647"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13151..13153),aa:Leu,seq:taa)
     gene            complement(13195..13266)
                     /locus_tag="VW324_001648"
     tRNA            complement(13195..13266)
                     /locus_tag="VW324_001648"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13232..13234),aa:Gly,seq:gcc)
     gene            complement(13289..13361)
                     /locus_tag="VW324_001649"
     tRNA            complement(13289..13361)
                     /locus_tag="VW324_001649"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13326..13328),aa:Thr,seq:tgt)
     gene            complement(13380..13461)
                     /locus_tag="VW324_001650"
     tRNA            complement(13380..13461)
                     /locus_tag="VW324_001650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13425..13427),aa:Leu,seq:tag)
     gene            complement(13467..13539)
                     /locus_tag="VW324_001651"
     tRNA            complement(13467..13539)
                     /locus_tag="VW324_001651"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13504..13506),aa:Lys,seq:ttt)
     gene            complement(13564..13636)
                     /locus_tag="VW324_001652"
     tRNA            complement(13564..13636)
                     /locus_tag="VW324_001652"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13601..13603),aa:Asp,seq:gtc)
BASE COUNT         4326 a         3015 c         2405 g         3891 t
ORIGIN      
        1 agtttactat gtcttttcac accaacctta ttccgaattc aattactttc gtgatttaca
       61 catatagatt aagactatct tttatacttt aaaatttctc gctaccttgt ccactatatg
      121 ctcctcgcta tcacgtttct attcatagcc tacgatttca ctattgcttt ctctgacaat
      181 tcttatttcc tgcgtcagac ttaaaacgat ctatccccag accattttaa tccgctacct
      241 cacgatagtc aggcttgggg agcgctattg tattcaccgg tagtggatcc ctacagagga
      301 cttacacctc agatgcacga catgcccatc gtataaaaaa atctcctacc caaggtagaa
      361 gatttcaaac ttataaaact taatccgtca tgtccgatac caacattcga tgctccaatg
      421 gaatactgca cataactagc aagaaaataa agcctgactg aatccagaag agagccaagt
      481 caaaaattcc gtgcacagca accactgtaa ggaaagatag ataaaggccg ataatcggac
      541 gtttccccga ctcctgactc atatccatca tcaagcgaac aggagcaaca gaagacaaaa
      601 ctaataaaat ggtacccaca attccgtaac tcagaatcgt atcaatataa agactgtggg
      661 catgttcatg ataaggagca tgtatccgag gataagagtg catataggtc aatggccctt
      721 caccccaaaa aggattttgc ttaaacaagg ccatcccagc atcccagata gaaatgcgtt
      781 cttccataga agagtctaaa gtacccattc gaactcccaa atcactagaa aagaggaaac
      841 tcaaaccaat cgcgaagacc ccaatactaa gccaaaaggc cttccagttt ttaatagtcg
      901 taaagagata gataattgct ccagcgataa tagcaggaaa ggcagttcga ttttgagtaa
      961 agttcaaacc aaagagattc acaaagcctg caatcacaca gaatactttc aaccaattca
     1021 acttggtcgt tgtaaacaga tagaaagcaa tcataataca gaaacaacaa ataattccat
     1081 aataattagg attaaagaag gtcacttctg cccggttctg atgccacacc tgcatattgg
     1141 gtgaaagaaa agcatagtta aatttcttca caatttggaa atgttctaaa ctggcaaaag
     1201 cagctgacaa gacactacca aacaagacga actgcaaaat caatcgaaag aatttatggg
     1261 ataaaatcga ctgatagtgc aaaaagaaaa tagtaaatag aaacattcct actgaagcca
     1321 caagacccat ccaattttgt gcaagaatgg atataacagt actatagcta agaaaaagaa
     1381 gcagcatcgg atgctccccc attttctgaa gaatactttt catgtctcct gtaaaaatca
     1441 aactgataat atataaacag agtacaacta caaaaagata aaagggtaaa aagatactta
     1501 ggataattcc caataaaatc agctctttac tagacaaccc cttcagcttt tcaataaagc
     1561 ctattgattt caaaatgaat cctttctctc caaatcagct gattcagata atagtaagct
     1621 atcctatatt gtaccacttt tttagcaatt tgaaaacaaa ggaaacgttt tccaaaataa
     1681 aaaccctatt ttatccacca tatcaaggct tcaaaatgat acttcaactc cattctcaat
     1741 tacccgataa gtctgatttt gcaaatcaat ttctactact gctgttacgg acttatcttt
     1801 attttgacgt ttgattacaa tgctgtgagc tgttggtgtc tctatctcag tagtcccttc
     1861 tagatcaaag gcttctgaac ggttacggaa agaaaataga ttgagaaggg ccttcacaac
     1921 aggtcgttgc acttcttttg ctatttcctc gttgctatag taatgacgat taatatttcg
     1981 accttcttta gtttcttcta ataatttcaa gtcattcttg cctgctaata gacccacata
     2041 gtaaacctga ggaatacctg gggcaaaagc ttgaattaga cgagcgagaa aatacttgac
     2101 atcatcatct ccaagcgctg aatagtaggt tgaattgatt tggtagatat ctaagttgtt
     2161 atactcggca ctagagtact tacgtttgac attggctcca accttataga gttcatttga
     2221 agcatagtca atctcctcat cggtcaggat atccttgaca tctactactc caatcccatc
     2281 atgggtatct agcgtcgtaa attgcttcat cgggctcatc tttaaccact tagccaaacg
     2341 ctctgttctg gaactgtaaa gagtataaag tgtcaccatt ggaagagcaa aatcataaac
     2401 atagtaatca tggtctgcta ttttaaactg aatcgaatag tgttcatgaa tctcaggtaa
     2461 aagctctgtc ccatactcag cagcgatatc tcgaactttg tccaataaat cccaaatatc
     2521 tggttccaca aagaaatcat tagtatccaa tttcttcact gcataagcaa aggcatctag
     2581 acgaatcaaa tcacacccat tacttgccaa gtgctgaatg gtcttacgga taaattccat
     2641 agttacttct ttggtcacat caagatcaat ctgctcctca ccaaaggtat tccacaaatg
     2701 ttccactgaa ccatcttcaa acacaatctc ttgctttggt gcacgatcct tacgcttgta
     2761 aattaaatct acatcagact gtgtcggacg gttttctggc caaaacttat cccagtttaa
     2821 aaagagagct ttaaattcac tggcttcatg tttttcttga tagtccttat aatacttgga
     2881 ttgacgagaa atatgattaa tcataaaatc aaacataaga taatatttct cacctaaacg
     2941 cttaacatcc tcccaatcac caaaagctga gtccacttcg tcgtagtcaa ctggcgcaaa
     3001 tccacgatca cctgttgatg ggaaaaatgg taaaaggtga actcctccaa tagcatctcc
     3061 aaaatgctct tccaaattat catataagtc tttaagatta tttccaaggc tatcagaata
     3121 ggtaatcaac atggttttat tttgaatcgg catcattact ctcctttttc taattgaagc
     3181 caagtctcat atgatctggc ttcgtaaatc aaattcattt taaatctcta tttatcatca
     3241 aactcgtact aatatagact gtgataaaca aagtactact ttcttgtttt ctacatagaa
     3301 ttatcaacaa gctgaactct tcctctgtgt caaagactat agattccatg agctcttctt
     3361 atactcttcg aaaatctctt caaaccacgt cagcttcacc ttaccgtagg tatggttact
     3421 gacttcgtca gtttcatcca caacctcaaa acagtgtttt gagcaacctg cggctagctt
     3481 cctagtttgc tctttgattt tcattgagta ttacttcact gccccgttgc tcattcctga
     3541 aatgatatgg cgttggaaga agagatagac aatggtgata ctgataatgc cgaccacgta
     3601 agaggcaaag cttggtccgt agtcgttgaa atattggcct gcgtagttgt attggaacaa
     3661 aggcagagtc cacattttgg aatcccggtt caagacaagg agtggcaaca tgaagtcatt
     3721 ccagaaccaa agggcattga tgatcatggt tgtcgcatgc atcggtttca tcattgggaa
     3781 gatgatgcgg aaataggttg taaattgatt agccccatcg atctctgctg cttcatccag
     3841 actttctgga atcgagattt tgatatagcc aacatagaga aagagggtct gtggaatcgc
     3901 ataggtcaag tagagcaaga tcaaaccaaa ggtattagcc aaaccgagtt tactcatcat
     3961 aaccgtaatc ggaatcatga tgacttggaa aggtacgaag attccgagga ttaagagggt
     4021 atacatgatg gtaaagactt ttcttttact catattgcga gcgatggagt aggctgccat
     4081 agggataaag atcattactg caagtaaaga caagacagtg atgacgacag agttccaata
     4141 atagcctcca atcccatcag ctaagagacg gctaaagttg tcccatgtga agttggttgg
     4201 aaagccaaag aaattatcta caatatcctt agtgggtttg aaggaactaa agagggtagc
     4261 aaggagcggc actaaaatca gaaccgatcc tagaatcaat agaatgtatt tgccaatcag
     4321 ggcttttctt tcatcttgtt tcatcttgtt tcatcatgct tctcctctta aatttcaaat
     4381 ttcttagata ctctcaattg gatgatcgaa atcactacaa ttaagaggaa caagattacg
     4441 gcaatggcat tggcataacc gaattggttg tttttaaagg catagttata aaccaagagc
     4501 ccaagtgagg ttgtggcatt gtttggacca ccaccggtca tggcaaagac ttggtcaaag
     4561 gcagtcagcc caccttttag ggctaggata aagaccatag agacacttgg tagcaagtaa
     4621 agcaattcaa tgttccagaa aacttgcttg ctagtcgcac catcaatcct tgctgcctct
     4681 gtaatctcag ttggaataga ttgcaaacca gctaggaaga tgatgatggg catagccacc
     4741 ccttgccaaa gaaggacaaa gacagccgca aagattgctc cccacttagt ccctaaaaga
     4801 ctggtttgga gaaattcaat atgaagggca ttcccaatcg ctggaagacc gtagttgaag
     4861 acttgcttga agatcaaagc cactgtcaaa ccagataaaa cagctgggaa gaagaaccaa
     4921 gcacggaaga aggtttggcc tttgatttta gagttcaaga cacgcgcaat gaagatcccg
     4981 agtgcaatct caccaaccac catggcaatc gtaatgattg cggtaaagcc aatcgcattc
     5041 atgaattttg gatccatgaa gaggagctta aagttgttta agccgacaaa tttgtagttg
     5101 taagtcaatc ctgtccagtt ggtaaaactg taaaaggctc cttgaaacat cggcacatag
     5161 aagaaaattg cttgtaacaa gagggggatg accacaaaag cccatgccca atatttttgt
     5221 aatacttttt tcatagtctc tctactccta atccacatcc gctttcatcg ggttaaagaa
     5281 ggcattcaaa tcattgacca tgccttgttt atcaccggtc aagacatagt tcatggtcaa
     5341 ggtatggaag tctgcttcac tggtccagta ttgttgcaac cagaccaagt gacgatccgt
     5401 aaaggcatat tcggtcatac cagcaagcgg tgaatcttct cctgcttgtt tgaccccttc
     5461 gatcgctgtt ggagatccgt ccacatcgta gtatttttgc atgacttctg gacgggtcat
     5521 atattccaca aaggcattgg cttcttttgg atgtttggtg gtggctgaga tagaccatgc
     5581 caagtctccc gcaccaacgg ttaagctttg tcctttttct tttcctggaa tcatgaaggt
     5641 tccaatctta aagttcggtt tttgttcatt aatcgctgtg atcgcccaag acccatttgg
     5701 tgtcatgagg acatccccac gtgcgaaggc tccgataaca tcggtatagc cagcaccttc
     5761 ccagttcttt tgcttagagc cattgatgcg aaggatgtcc atgaccttga tatcatcttt
     5821 cataatcgga tccgacaatt taatggcatt tggttgagaa taacgaaggt attgatttgc
     5881 ttcttttcct ccacctgttg ctgtcgcaaa ggctaattga ttgtaaccat tgagtgtcca
     5941 agcatctgca cctgcaattc caaatgatgt ttgtccttta gcaacgatat ctttgactaa
     6001 ctgttcaaat tcatcccagg tttcaggaac cttcaagccc agttcttcga atttatcttt
     6061 gttgtagtaa attccataag cattagctgt aaaaggaacg ttataaactt tttcgtttac
     6121 agcatatttt tcagcgtagg catttttcac gcgtttcagg tagtctttgt tgctcaaatc
     6181 ttcaaaaaca cctgcttttg cccattcttg cagttcgatg gactgtgggt aaatattgac
     6241 cacatcaggc acatctcctg cgagaacgcg tgtcttcaat acttcaccag catttggtac
     6301 attgacgact ttgaccttga tcttagggtt tccttctcaa aatcacgagt gatttcttcc
     6361 aaggttttgg tcatttcttt tttctggttg aaatactcga tggtcactgt gccatccgca
     6421 gatttaccat agttggagca agcgccgagc ccaaacaaag ctaaacctgt agttgcaaga
     6481 agtccgattt ttttatacca ttccattaga aagcctcctt tataaattta tacaccctta
     6541 ttgaactgca ccccaaaagt tagacagaat aaatctaact tttggggtca gtacatatca
     6601 tagttttcta aaaatatact gtctactcaa aaaatctcct tgggataaga taacagttaa
     6661 gcccgcatac attagttctg cacctgagta aacttcgcca ttttcctgta atttatatag
     6721 tccctcttca tccaaatctt ttaattttaa agttgtttcc atggtctcta caacagataa
     6781 aacgcgaacg taggttacaa tcgtttgatt tccgtaatta aattgtacag ctgcttcatt
     6841 ggatgcagta tcaggattaa ttagtctata ctgctgtcct aactgaacta ctggtcgtaa
     6901 ttctttatac aagttcacct gattagcaat cgtagctttc tcttcatctg ataaatttgt
     6961 caaatcaagc tcatagccca aatttcccat cattgctaca tggccacgtg tttctaatgg
     7021 tgtcattcgt cccatctgat gattcggtac tgctgacaca tgaaccccca tagaaatggt
     7081 tggatagaga taggatgaac cgtattgaat tggtaaacgt gcaatggcat cagtattatc
     7141 actagcccag acttgtggga aatagcgtat cataccaaga tcatttcgtc caccaccacc
     7201 agagcaggac tcaaagagaa tatggctgtg cttctctgtc agataagaaa cgagttcgta
     7261 aagccccagc atgtactgat gagattgcat ctgtgtctct agataagtta atccattccc
     7321 tagcttagtg atattgcggt tcatatccca tttaatgtaa tcaatctcat gataagatag
     7381 gagttgatct aagacacttt tcaagtattc tactacctga ggattggcaa gattaagtac
     7441 taattgattc cgagaataag tatgctcata atcaggaacc tgaatagccc agtcaggatg
     7501 ttgacgatac aaatcactat ctacagaaat catttcgggt tctaaccaaa gtccaaactg
     7561 caaacctctt tcatggatag ctgaaatcag actttctaga cttccaccca gtttttcctc
     7621 attaacaacc caatcaccta aagcacgatt atcatcaaaa cgattgccaa accaaccatc
     7681 atctaataca aaaagttcaa tgccaacttt cttagcttca tctgctaact ctaacagttt
     7741 ttctctctga aagtcaaagt aagtagcttc ccagttattg attagaattg gacgttcttt
     7801 tttagaaaat tcacttagca taatgtgctt cagtacaaaa ttctgacttt catgactaat
     7861 accagttaat ccctgatctg aatgagtcac taaagctacc ggtgtttcaa agtattcctc
     7921 aggggctaac ttccaagaaa agttttctgg attaatgcca atagccaccc gaacttcatt
     7981 caattgattt ttttgaacaa aagcttcaaa gttgccacta tacattagtt gaatagcaaa
     8041 cacattccca gcatcctctg tgactccttg ttcgcatagt agaagagctg gtgtttgagc
     8101 atgaccagaa gcacctcggt ttgaactaat cgaaaagatt ccttgttcta cctgttgacg
     8161 tctaacagtc ttttcacgag cataagcacc ctgcagagtt gctatttcat aatctgcagc
     8221 tggaaaatca gccataaaag aaaaatcttt atggatgaca acttcctgat tactattatt
     8281 atctaattta ctgtagctag taatagtcgc atcattatta aaagtagtat aatacaaagt
     8341 cagactaagt tgagccttag aatcttctaa cattaagaca agagtctctg tatcgtccat
     8401 gctatgtgga gaaggtaagc cctgtggacc attctgacct tttaaaatct ttgcttctac
     8461 aaatcgaaag tctgttactt cagttacact atgctgaacc tgtatagttg gtttcctaaa
     8521 atctcctaag ccatgttgtc caaaaatctg tcgctgagta tctaaactaa aggttcgatt
     8581 agtagccgtt ggatttcctg aaaaggcatg gtctcgttca taaacactat tggaaccttt
     8641 atagttctta atagtctttc ctaaatgttt caaaagtaag tagccatttc gattttcaat
     8701 aatcaaactt agatttttac tctcaacata aaatagatta ttctctatcc taactcccat
     8761 ttacttcacc tcatcacttt attgattata ttttatcacc tgaaatcgct ttccaaaata
     8821 gaaaaatgtc tcaagaatat ggtaaaatgt taggtaggag gtagcacatg ttagtttttt
     8881 cagaatacca gactggaaca atcgaccttg ccctaagctt ttatggatat gaggaatgca
     8941 cacctaatta ctcttttggt ccagccattc gtgatacata cgtcctacat tacattacta
     9001 aaggacaagg aaaatttcat tacaaggata aaattgttta tttaaaagaa ggagatttct
     9061 ttctattaaa accagaggaa ctaacctttt atcaagcaga tagtaaagaa ccttgggcct
     9121 actactggtt aggaatcact ggagggaaat cccctgatta ttttgctctt tctcaaattt
     9181 ctgatcaatc ctatctcatc caatctgaaa cttgtcatac ccagactact gcaaaactca
     9241 tctcagacat tgtccgcttc gctcagatta caaaatcaag tgaattagct caactccata
     9301 tcatgggaca acttcatgaa ctgatgtttc atctgggaac tattgctccc aatcagaaaa
     9361 aaaagaatat ttcatcaacc caccaactct atcttgaatg caaacgatta attgatagcc
     9421 actatcctca atcacttaca attcaagatt tagcaaaaga actatccgtt cacagaagct
     9481 acttatcaag cgtattcaaa gaatttaata ccttatcacc caaagaatac ctactctacg
     9541 ttcgaatgca ccgagctaga caacttctcg aaaataccca agagtccatc aaggtaattg
     9601 catactcggt aggtttttca gatccactcc atttttcgaa agcttataaa caatacttta
     9661 atcagactcc aagtcataca agaaaagaat actctcaata ccaactagtg agaaaggcaa
     9721 cattatgaaa tcctaccaag ctgtctacca aatcctatct aaagaaaccg actatatcag
     9781 cggagaaaaa atcgcagaaa aactatccct aagccgaaca tcaatttgga aagctatcaa
     9841 gcgactagaa caagaaggca ttgaaattga tagtatcaaa aatagaggat ataaactaat
     9901 gaatggtgac cttattcttc cagagattct agaagaaaat cttccaatta aagtcagctt
     9961 taaacccgaa acaaaatcaa cacaactaga tgcaaaagaa gcaattgatt taggccatga
    10021 agcaaatacc ctctatctag cttcctatca aacagcaggc cgaggacgtt ttcaacgttc
    10081 cttctactca ccacaaggtg gtatttatat gacactccat cttaaaccaa atctccccta
    10141 tgacaaatta ccatcctaca cactacttgt agctggagct gtctacaaag ctattaagaa
    10201 cctaacttta atagatgtcg acataaaatg ggtcaatgat atctatctaa acaatcataa
    10261 aattggagga atccttactg aagcaatgac ctctgtagaa actggcttag tcacagatat
    10321 cattattgga gtaggtatca atttcactat taaagacttc cctcaggaat taaaagaaaa
    10381 agctgccagc ttatttaaag ctacagctcc tataacaagg aatgaattga tcatagaaat
    10441 ctggcgtgct ttcttcgaaa caccagcaga agagctatta tacctataca aaaaacagtc
    10501 attcattcta ggaaaagaag tcactttcac actacagcaa aaagactaca agggacttgc
    10561 taaagacatc tcagaaaatg gaaaactttt agttcaatgt gataacggaa aagaaatctg
    10621 gctaaatagt ggcgaaattt ctctcaatag ttggaagtaa aataacacaa ttataatata
    10681 aggctctata atatttgtag tgggtaaatc ccctatggat attatggagc ctatttttgt
    10741 gtagaaaaaa agtcccataa gatctataat gaaaagcgat caaacaactc attagaaaga
    10801 ttcatatgga acaattacat tttatcacaa aactactcga tatcaaagac acaaatatcc
    10861 aaattataga tgtcgtcaat agggattcac acaaagaaat catcgccaaa ctggactacg
    10921 acgcaccatc ttgccctgag tgcggaaacc aattgaagaa atatgacttt caaaaacctt
    10981 ctaaaattcc ttatcttgaa acgactggta tgcctactag aattctcctt agaaaacgcc
    11041 gtttcaagtg ctatcactgt tcaaaaatga tggtcgctga aacttctatc gtcaagaaga
    11101 atcaccaaat ccctcgtatc atcaaccaaa aaattgcgca aaagttgatt gagaagattt
    11161 ctatgaccga tattgctcat cagctggcca tttcaacttc aactgttatt cgtaagctca
    11221 atgattttca ctttaaacat gatttttctt gtcttcctga gattatgtct tgggatgagt
    11281 atgcttttac aaagggaaag atgagcttca ttgcgcaaga ttttaacaat ctcaatatca
    11341 tcactgttct tgagggtaga acacaagcta tcattcgaaa tcactttctt cactacgata
    11401 gagctgtccg atgtcgcgtc aaaatcatta ctatggatat gtttagccct tactatgact
    11461 tggctaaaca gcttcgcttt caaatttcta ggctcaggct gaaacagtct cccagactgt
    11521 ttcactcccg aatgctaaaa tcgttcttga tcgctttcac attgtacaac atcttagccg
    11581 tgctatgagt cgtgtgcgtg ttcaaatcat gaatcagttt catcgaaaat cccatgaata
    11641 caaggctatc aagcgctact ggaaactcat tcaacaggat agtcgtaaac tcagcgataa
    11701 acatttttat cgccctactt ttcgtatgca tttaaccaat aaagagattt tagacaagct
    11761 tttgagctat tcacaagact tgaaacacca ctatcagctc tatcttgctg tttcactttc
    11821 agaataagga accagagaaa tttttcggac ttattgagga caatcttaag caggttcatc
    11881 ctatttttca gactgtcttt aaaaccttcc tcaaagataa agaaaagatt gtcaacgccc
    11941 ttcaactaca ctattctaat gccaaactgg aagcgaccaa taatctcatc aaacttatca
    12001 agcgcaatgc ctttggtttt cggaactttg aaaacttcaa aaaacggatt ttcatcgctc
    12061 tgaacatcaa aaaagaaagg acgaaatttg tcctttctcg agcttagctt ttcttcaacc
    12121 cactacagtt gacaaagagc cttaagttta cggatctgaa gttttattgt ctctaaaaac
    12181 aaaaaagaga gttacaaact ctcattaaaa cggagaataa gggattcgaa cccttgcgcc
    12241 agttacccga cctaacgatt tagcaaaccg tcctcttcag cctcttgagt aattctccaa
    12301 ttaatgggca cgagtggact cgaaccaccg acctcacgct tatcaggcgt gcgctctaac
    12361 cacctgagct acgcgcccaa gttaaaaaac ttggtaattt gaacaaagtt caaagcgggt
    12421 gacgagaatc gaactcgcga caacagcttg gaaggctgta gttttaccac taaactacac
    12481 ccgcataaat actatcaata aaatggcgcg agacggaatc gaaccgccga cacatggagc
    12541 ttcaatccat tgctctacca actgagctac cgagccttat tgcgggagca ggatttgaac
    12601 ctacgacctt cgggttatga gcccgacgag ctaccgagct gctccatccc gcgttaatat
    12661 aaaaggagga tgtgggattc gaacccacgc acgcttttac acgcctgacg gttttcaaga
    12721 ccgttccctt cagccggact tgggtaatcc tccaatattc aaatggacct tgtaggactt
    12781 gaacctacga ccactcggtt atgagccgag agctctaacc agctgagcta aaggtccgac
    12841 aagatcatta tagcggcgaa ggggatcgaa cccccgacct cccgggtatg aaccggacgc
    12901 tctagccagc tgagctacac cgccatgaat cgggaagaca ggattcgaac ctgcgacacc
    12961 ttggtcccaa accaagtact ctaccaagct gagctacttc ccgagttaaa tagaaaaatg
    13021 caccctagag gagtcgaacc tctaaccgcc tgattcgtag tcaggtactc tatccagttg
    13081 agctaagggt gctccatatt atgccgagga ccggaatcga accggtacga tcgttaccaa
    13141 tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca ccccggcctc tctaagcgaa
    13201 cgacgggatt cgaacccgcg acccccacct tggcaaggtg gtgttctacc actgaactac
    13261 gttcgcactg ttttcttcta tctaaaaatg ccggctacat gacttgaaca cgcgaccctc
    13321 tgattacaaa tcagatgctc taccaactga gctaagccgg ctcatttgtt atatcttaat
    13381 gcgggttaag ggacttgaac ccccacgccg ttaagcgcca gatcctaaat ctggtgcgtc
    13441 tgccaattcc gccaaacccg catatatgac ccgtactggg ctcgaaccag tgacccattg
    13501 attaaaagtc aattgctcta ccaactgagc taacgagtct aaaataactt gcgttacctt
    13561 aaacggtccc gacgggaatc gaacccgcga tcttcgccgt gacagggcga cgtgataacc
    13621 gctacactac gggacct
//
LOCUS       DAVKLW010000054        12896 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKLW010000054 DAVKLW010000000
VERSION     DAVKLW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.054"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            122..277
                     /locus_tag="VW324_001653"
                     /pseudo
     CDS             122..277
                     /locus_tag="VW324_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VW324_001654"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VW324_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9727237.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYIDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERKNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQPITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VW324_001655"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VW324_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9727238.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7614..8792
                     /locus_tag="VW324_001656"
     CDS             7614..8792
                     /locus_tag="VW324_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727239.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            9001..>12896
                     /locus_tag="VW324_001657"
     CDS             9001..>12896
                     /locus_tag="VW324_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9727240.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEPSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPS
                     AEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHN
                     FINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVI
                     KLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQ
                     YQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVY
                     DELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENY
                     FMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKT
                     QQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGT
                     NNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHA
                     DGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLID
                     KSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLW
                     DPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEI
                     LTKDSTVQRGEVLEDAQIKEKKATFVSKNTIKTEDF"
BASE COUNT         4282 a         2457 c         2705 g         3452 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgaatt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc gccttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgctga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcccgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga cttgtatatt gacctcagtc
     1681 agtctgatat tgatgctttt gaacaatata tccgctatct tggtattaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttttg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggaaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagaa
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcagcc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggttgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtgcttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgagctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag ataaccattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatcctcaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac atgcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg aattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgctt tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggtttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagtca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agtactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt tgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgattgcgg
     7561 ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac
     7621 ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg
     7681 aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc ggccgtagca
     7741 attgtgtctg tggaaatgcc atcaaatacg cttatgaact ctttaacggt gtcacaggcc
     7801 aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc
     7861 agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc
     7921 agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt ctaaaatggc
     7981 tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact
     8041 accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa
     8101 aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg
     8161 atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc
     8221 gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca
     8281 atctggttct agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag
     8341 agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag
     8401 aacgggctat caaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca
     8461 gtcaggatcc aattgaaaga aaacagaagc agacttggct cctcaattcc tattttcgtg
     8521 agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga aaaagtggtg
     8581 aagtaccctt ttcaactgaa tacctgtccg atcatctcat cgactttttc tataaaatga
     8641 aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc
     8701 aggaagacca tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc
     8761 tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt
     8821 aggtgttatt gtacccccaa ataatataaa acaaagtatg aaagcgcatc tcaattttaa
     8881 gacttttttg taaattaaga gtataattaa ggtatactgc cttttctata gataatagat
     8941 aatacactaa tataataaag gtctttgtaa catatataaa ttttatttca aggaggaata
     9001 atggaaaagt attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga
     9061 cttgtatctg caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg
     9121 gatgctcaag aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca
     9181 gaagaaaaga agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact
     9241 tattatcttg tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga
     9301 agcaagaatg agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta
     9361 atttttgctg tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt
     9421 gcgggaatgg gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta
     9481 aattacaata cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt
     9541 tcaggttaca cttatattgg atatatcaaa gagggaaaaa cgacttctga ttttgaagta
     9601 agtaatcaag aaaaatcagc agccactcct acaaaacaac aaaaggtgga ttataatgtt
     9661 acaccaaatt ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt
     9721 tcttcaacta agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc
     9781 aatccaagaa aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag
     9841 aatctagaaa cgaagaaaga ggagaagatt tctccaaagg aaaagactgg ggtaaataca
     9901 ttaaatccac aggatgaagt gctatcaggt cagttgaaca aaccggaact cttatatcgt
     9961 gacgaaacta tagaaacaaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta
    10021 gcagaaggaa ctgtaagagt aaaacaagaa ggtgcattag gtaagaaagt tgaaatcgtc
    10081 agaatatttt ctgtaaacaa tgaagaagtt tcacgagaaa ttatttcaac ttcaacgact
    10141 gcgcctgttt caagaatagt cgaaaaaggt actaaaaaag ctcaagttat aaaggaacaa
    10201 gctgagactg gtgcagaaca taaggaagta cagtctggag ctattgttga acccgcaatt
    10261 cagcctgaac taccagcagc tgtattaacc gacaaaggtg agtcagcagt ccaaccagag
    10321 ttgccagaag ctgtagtaag tgataaaggc gtaccagaag ttcaaccagc gttaccagag
    10381 gcagtcatga ctgacaaggg tgatccagag caggtagaac cgctaccaga atatacagga
    10441 gtacaagctg gagctattgt tgaacctgaa aaagttgaac cggaatatgc tggagtacaa
    10501 gctggcgcta ttgttgaacc agagcaggta gcaccactac cagaatacac aggggtgcaa
    10561 gccggagcga tagtcgagcc agagaaagta gaagcaccga aggaatacac aggagtgcag
    10621 gctggagcga tagtcgaacc agagaaggta gaagcaccga aggaatacac aggagtgcaa
    10681 gccggagcaa tagtagaacc agaaaaagta gaaccatcaa aagaatacac aggggtgcag
    10741 gctggcgcta ttgttgaacc agagcagata gcaccactac cagaatacac aggggtgcaa
    10801 gccggagcga tagtcgagcc agagaaagta gaagcactga aagaatatac tggtaaaatc
    10861 gaacaaccta gtgcagaaga tactaaacca aataatgaaa atactaatac accagaagaa
    10921 atgagtattc agaaaaaatc tagtgcatta attaatatga actttattac agattcgtcg
    10981 aaggttactg gagtaggatc agctactttt attgcaccaa atgtactttt aacagtagca
    11041 cataatttca ttaataattc tactgataat actactggcg aatttagagg tgataaatct
    11101 aaaaacgtat atgagtgggt gactcctgat ggacaaaaag gaacatttac tgctaacaat
    11161 attcattttt ataataaaaa agattatcca aaaggattta tctatgattt agcagtgatt
    11221 aagctacctg aaacaactgg cagagaacat gtagaattag ttaaaaatta ttcgaaagta
    11281 aatcttaatg acaaactaaa tgttcatgga tatccagctg ggaaatatac gcacttaaaa
    11341 gatgctacgg tagaaatgga acaagaatat gcgaataaca cctacggtgt tcaatatcaa
    11401 ggtggaaatc ctggtatgag tggtggagga atatttaatg ctaatggtga agttattggt
    11461 gtgcatcaaa atggagcaca aaatcgctca ggcggattga ttttatctcc tacacaatta
    11521 gcttggatta agagtattat agcaggtaat gaaattccac cagtatacga tgaactttat
    11581 cgtcataaag atgagaaaaa agatgacgct aaagatgaaa aagaagttat taagaaatta
    11641 gaacttagaa atatatcttc tgtagaacta tattcaaaag atggtaataa gtatcgacat
    11701 gtaacttcac ttgcttcttt accaagtaat gcagaaaatt actttatgaa agttaaatca
    11761 gagaacttta aagatgtgat gctaccagtt acaagcatta ctaatgatac taaagataat
    11821 agagatgttt ataagatagt agcaagtgca aatagtctga tacaacatga aaataataat
    11881 gtactagaaa attatacata ctatttacca aaaactcagc aaagtgaaac tggggtatat
    11941 acatcattta aaaatttagt agatgcaatg aatagcaatc ctaatggcac attccgttta
    12001 ggagcaacta tggatgctcg tgaagttgaa cttccagatg gtcaagaaag ttatgtgaat
    12061 aatgtattcc atggaatatt agtaggaaca aataatgaga aatattatgc tatttataat
    12121 ttgaaaaaac cattatttgg ggaattaaat ggtgctacag tagaaaaact ttcactaaaa
    12181 gatgttaata tttccgcaaa ggacgatact gcaacacttg cgaaagaagc aaataacaat
    12241 actcatattg ataacgttca tgctgatgga gcgatagctg gggaacgtag cataggagga
    12301 ttagtatcac aagttaataa ttctactatt tctaacagta gctatacagg tagaataact
    12361 aatacatata aaacagttgc tagttatcaa atcggaggtt tagtaggcaa actttctggt
    12421 ccgaggggat taatagataa gtcgttcgca tcaatcgatt tatcttcaaa tgcaacacaa
    12481 ggagaccagt ctattggtgg aatagttggt gcagtagaaa atagtgcgct tattagtaat
    12541 agttatgctg aaggaaatct aaataatgtt caacgttttg caaacgttgg tggagtagtt
    12601 ggtaatcttt gggatccggt tggaggatta gaaaaatccg gtcgactttc taatgtacta
    12661 agcgatgtta atgtaacgaa tggtaatgct attgcaggtt ataattttaa tggtataaaa
    12721 gcaaatggca catacagtaa taaaaataat aaggttgtga acgtagtaca agaagatgat
    12781 gaaatcttaa ctaaggattc tactgttcaa agaggagaag tattagaaga tgcacaaata
    12841 aaagagaaaa aagctacttt tgtatctaaa aatactatta aaacagaaga ttttaa
//
LOCUS       DAVKLW010000055        12876 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKLW010000055 DAVKLW010000000
VERSION     DAVKLW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.055"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(383..2110)
                     /gene="ezrA"
                     /locus_tag="VW324_001658"
     CDS             complement(383..2110)
                     /gene="ezrA"
                     /locus_tag="VW324_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9727241.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2192..4138)
                     /gene="gyrB"
                     /locus_tag="VW324_001659"
     CDS             complement(2192..4138)
                     /gene="gyrB"
                     /locus_tag="VW324_001659"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9727242.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4153..4716)
                     /locus_tag="VW324_001660"
     CDS             complement(4153..4716)
                     /locus_tag="VW324_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9727243.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4801..5355)
                     /locus_tag="VW324_001661"
     CDS             complement(4801..5355)
                     /locus_tag="VW324_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9727244.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5366..6589)
                     /locus_tag="VW324_001662"
     CDS             complement(5366..6589)
                     /locus_tag="VW324_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9727245.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6626..9076)
                     /locus_tag="VW324_001663"
     CDS             complement(6626..9076)
                     /locus_tag="VW324_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9727246.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVGHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELSQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9276..10205)
                     /locus_tag="VW324_001664"
     CDS             complement(9276..10205)
                     /locus_tag="VW324_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9727247.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10345..10632
                     /locus_tag="VW324_001665"
     CDS             10345..10632
                     /locus_tag="VW324_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9727248.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10666..12000)
                     /gene="ciaH"
                     /locus_tag="VW324_001666"
     CDS             complement(10666..12000)
                     /gene="ciaH"
                     /locus_tag="VW324_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9727249.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11990..12664)
                     /gene="ciaR"
                     /locus_tag="VW324_001667"
     CDS             complement(11990..12664)
                     /gene="ciaR"
                     /locus_tag="VW324_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9727250.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3916 a         2745 c         2292 g         3923 t
ORIGIN      
        1 gtgcaaataa gaaatctcca gattaggaac tatccgtgag ttctctagtc tggagatttt
       61 tcaatagact tcgttattgg acggttacca agttactcaa agtacagatt tgaagctgca
      121 gataagactg acgaaatcac tttaatacta aatgaaaatc aaaaagcaaa ctaagaagct
      181 agcctcaggc tgtacttgag tacggtaagg cgacgctgac gtgatttgaa gagattttcg
      241 aagagtataa aacactgttt tgaggtggtg gataaatcgg ctgggtaaaa actcaatctc
      301 aggagaaaat gaagtaaatc tccccacaac tatagaaaaa gatttgattc tgctcctcac
      361 acaataaaat ctttttcttt tattaaaaac gaatcgtttc acgtgttttc tcatatgagg
      421 taacaaagcg attggttaca ccaggctctg ccacttccaa tgcttgagaa atcttgtcaa
      481 atgaagcgtg ataatcaaat tctttttcaa aaatatctaa agcttcgtta aatgcttctt
      541 gaatgcgttc atcaaatgag cgatagcggt tagaatattg caagagttgc tctgtcaaag
      601 ttgcatattg tacaatatta taagtttccg tttctaaagc ttccatatca ttcgttgcaa
      661 tttcaagaac tcgggtaaca gattcaatgt taatcatttt ttgttctaac tcaaccatta
      721 aatcctcggt attattgctt gccgtaaaga ataacttcaa gaaagtttgt ggaatacctg
      781 gcagattgcg tttttccatg tatcgcttga tagtatggag acgattgaca taaacattgg
      841 ccttttgacg tgcattaata tcatctttct caatttgtgt caggcgctca ctaactgaaa
      901 tttgctcatc ttcaatatct tttagttgag tttgtaaatc ctcaagattt tcttcaagaa
      961 ctgaataagc ttgggttggt tcttcttgat ttgaagttac ctcaacaata gctgcctcaa
     1021 aactctctaa ttctgtctga atacgacgaa catggcttgc agctgtctca ggaagtaaat
     1081 aggtcttgtt caaacgtgca atatcttctc ccaataaagt attattctct ttcatatgtt
     1141 gaaggtaagt tggaagagtt gcaagtagat tttccactac tttctgagca gcaatttctc
     1201 gagtaaaaat atcatacaag gcattgattt cctcttgtgc ctgtccattc tcatattcgg
     1261 cattatccaa ttccaactga cgaatattct cttggttttt cttgaatgct tcataaagca
     1321 agtggaaacg cgcttcaata tccgtttcaa caaaatgata attagcatca attagtttac
     1381 gataaccgga ttccaaatcc tgtaattgat ctggcaattc tgtagaaagc gtcgtaacca
     1441 aggctggaac acgatccaca atatgactta aggccaaaat gtgattttct gtattatcca
     1501 aaatcactgc ggcttccaca gggtcacccg atgaattcaa ggttacaaat tgtgaaaatt
     1561 cagattggat attttctaat tgtttttcaa tttcatccaa ggcttgacca tactgttctg
     1621 aattttcagc aactctatgc tgaagttcct caaataaatc caaagcatga agaacacgac
     1681 cactattttt agattcttgc ttctctaagt ctgccaaagc attgcgaatt gccgcaatat
     1741 cttcgtcaat caaagtaatt tgactctcaa tttggtcaat ttgatgactg gccttgagaa
     1801 aacgaaatga atggttatag ccttctgctt caaagagatt attttcaata tcggcaaaag
     1861 agttgagaga taaatcgacc catttttgat tccattcacg gaaagccact tgactttgtc
     1921 caatcaagtg catatttttt acagcttcta cttcatcatt tactggaaga ttgtatagtt
     1981 cttcttttct ttcttctagc gcctctaatc tcccctcgtt tcgcttacgt agaaagattg
     2041 ccactacata agccagaact aaaatgactg caattgcaac cattaaataa attagttgtc
     2101 cattagacat atcaaactcc ttttttactt gaaacaatcg taacgattat atcatatttt
     2161 ttagaccaag ggaactactt ctccctattt tttagacatc aagtgtacta tagacagcat
     2221 tttcttcgat aaactcacga cgaggctcta ctcgatcccc catcaacata tcaaagattt
     2281 tatctgcttc tgcagcatca tctacagaaa ctctagccat caagcgatgt tcgggatcca
     2341 tggttgtttc ccacagctga tgatcgtcca tttcacctag ccccttataa cgctgaatag
     2401 tcggtttggt acgaccttca ctataacggg ctaaagcttc ttggagtttg atttcttgat
     2461 ctgcacccgg ctggatatat tctttaatct cgcttccaac cttgacacca tagattggtg
     2521 gttgggcaat ataaacataa ccagcttcta ggattggttt catataacga taaatcaagg
     2581 ttaaaagaag ggtacgaatg tgggctccat cgacatcggc atcggtcatc aaaacgagtt
     2641 tttggtaacg ggctttcgaa acatcaaatt ctgcgccaaa tcctgttccc atggctgtga
     2701 aaagactacg aatttcttcg ttagctagaa tcttatccat acttgctttt tcaacgttca
     2761 aaatcttacc gcgaattgga aggatagcct gaaactcacg gttacgacca gatttggctg
     2821 atccaccagc tgagtctcct tcgacgatga agagttctgt ttcagcaggg ttattagaag
     2881 aacagtctgc tagtttccct ggaaggttgg aaatttccaa accagatttt ttacgtgtga
     2941 cttcacgcgc acgcttggca gccacacgag ccttggcagc taaaatccct ttttccacga
     3001 tacgtttggc aatctgtgga ttttccatga ggaaatcgga gaaggcttca ctgaagaggc
     3061 gattggtaat cttgaccact tcgctatttc ccaatttggt cttggtttgt ccttcaaact
     3121 gtggatttgg gtgtttaact gagataactg cagttaatcc ttcgcgaaca tcttcccctg
     3181 ttaaattatc ttcattgtct ttcagtaact tattcttacg agcataatca ttgataacac
     3241 gagtcagtgc cgtacggaaa ccttgttcat gcgttccacc ttcatgggta tgaatattat
     3301 tggcgaaact catgacattt tcatggtaac ctgttgtgta ctgcatggct acctcaactg
     3361 tgatatcatc catctcaccg tctgtataga ttggtgtatc aaagattaca tccttgttct
     3421 cgttgatata ttcaacgtaa ctagcaatcc caccttcata atgataatgc ttggtttgtt
     3481 ccaaaccttg gcgcttatct gtaattgaaa tttgaagacc gcgatttaga aaggccaact
     3541 cttgaatccg tttatttaat ttatcaaaat caaagattgt tgtttcagtg aaaatttctg
     3601 ggtccggtgt gaagtgaacc gttgttccag ttctatctgt atccccaacc acctcaaggt
     3661 ctgcaacaac atgaccacga cggtattctt ggtaatgaat cttaccgttt ttatggacat
     3721 gaacgtctaa ttgagtggaa agggcattaa ctactgacga ccccaccccg tgaagaccac
     3781 ctgaaacctt gtatccacca ccgccgaact ttcctccagc atgaaggact gtaaagacgg
     3841 tctcaacagc aggacggcct gttttttcct gaatatcgac tgggatacca cgcccatcat
     3901 ccacaacagt aatcgaatca tctggctcaa taaaaacttg aatatggctg gcaaatcctg
     3961 ccaaggcctc gtcaattgag ttatcaacaa tttcccagac tagatggtga agaccttctt
     4021 ttgaggttga tccaatgtac atccctggac gcatacgaac agcctctaag ccctctaaaa
     4081 cttgaatttg actggcatca taatcctgtg cctgcagatt tttgatttct tctgtcatct
     4141 tattcctttt tcttatatgt ctaatacttg tcttaaattc acgatactgg taaacaagtg
     4201 ggtatcaagt cctgcagctt gacctgcttc gatatcaatc ggccgatcac caatgacaag
     4261 accagagcta atctgatact tttctcttaa ataaagcatg gattcgggat ttggctttct
     4321 cttaaagcct gagctagaag tcaccacttc tgtaaaataa gctgctatag aggttttttc
     4381 taaaatttcc aaaacctgat catttcgatg agagaccaaa aaatgacggc caccttgatt
     4441 taaaatgtct tccaataggt cagaaactcc ttcaaataaa atcgggtgtt caagctctct
     4501 ggcttcattt tccttgtact tttctaaaaa attctctaaa ttgggagcga atgtctcaat
     4561 cgcaaaagga gtagaaacct ttaaagcttg atagacactg tcatggtctt gtgtgatacc
     4621 atacagtgcc aatgtttcaa caaatgcagc tgttgaagtt tcataattat ccagtaaagt
     4681 tccacctaaa tcccagatgt aatcgtgata tttcataccc ttcattatag cattttttta
     4741 tgaaaaaagc gatgatttcc ctgagttttc cacataaaaa acgagagaat aactcccgtt
     4801 ttactgattt ttaccaaaga catctcgata gagcattcct gttcgtaaac tctctgcgtc
     4861 ccctcctagt tgctccacca actcgtaggc aaaggcaagg gctgttgaag gaccccgact
     4921 ggttgtcaac tgaccatcta ccactactgt ttccttgacg tagtgaccat caaggatttg
     4981 ctcttgaacg ccgtcataac aagtgtatcg cttatttttc aatatctctg cttgattgag
     5041 ggcaattggt gccgcacaaa tggctgctag tttcttccct tcttgctcga agctttgcaa
     5101 ttcttgaatc aaggtctgat tatcacgtaa atgtgcagaa ccaggcatac ctccaggaag
     5161 aacaatcata tcatagtctg ataaatctcc atcaaagaca tgatctgctc ttacttggat
     5221 tgcatgcgaa cccgttactt gctcttcaaa accaaccata tcacatgtga tattggctcg
     5281 acgcaagaca tctacaactg tcaaggcttc aatttcttca aagccctgag ctaacataac
     5341 tgctactttt accatgattt tctccttatt taatttgttt taatacaacc tttttccgtt
     5401 tgaatgggac ttttccgctc ttttcttcag ctaaattagt ctggtaactc atcgataaaa
     5461 gcaaaccaac accaatcaaa ttactgataa tagctgatcc cccttgcgaa atgaaaggca
     5521 agggaatccc cgtcaaagga agtagtccag tcacagcacc gatattctca aagatgtgga
     5581 agagcaacat cataatcaaa cctgtggaaa tataagtgta gaactggtta tttgatttaa
     5641 gagtaatctt caacatacgg taaatcaaca tgagatagag ggcaataacc aggacagagc
     5701 caataaagcc aaaatcttct gcaataaccg taaaaatcat atctgactct cgaactggga
     5761 taagcagatt cgaagcatta aatccctgac caaataagcc accactccca atggcaatct
     5821 gcccttgagc ctgctggtaa gtcgttgttt gggcaaactc aaagggattg agccaagcca
     5881 aaatccgatt aatttggtaa gtcggcattc caatctggtg aagaaaagct cgtccgtcct
     5941 tgctaataaa gatagctaag aaaccagcaa ctcctgttac agcagtcaca aatactggga
     6001 taataatttt ccaagaaacc cctgataata aaacgattcc tgagaaaatg gctacaaaaa
     6061 ccaaagccgt ccccaagtca ctttgaagtg ctaaaagaac taggactgga atggtaaaga
     6121 gaatcatcca gaaaattaac aaaaagtcca gcggaaccgt gcgtctccat tccttatgtt
     6181 tctttgtaaa ttggacaatg acacgagcca acatgaggat ataggatatc ttcataaatt
     6241 ctgacggttg aaatagggta attccattta ttgataccca gtttttggca cccgttgatg
     6301 caactaagct tggattataa aatacaatcg gcaagatcat aagtcccaag cctaaaatat
     6361 atagaaaggg ggtcaccttc caaagaaatt ctgtattaaa gagcatgacc acaaaaccaa
     6421 tcacaagccc caaggcaatc caggcgacct gctgccctaa aatgggcaga atattattgg
     6481 gataatcatg actaacggct atatagatag ccaccacacc gatgaccagt agaaaaaata
     6541 ctggcaagag caaactataa tcgactctag agtcgagaga acgtttcata tgtactaacc
     6601 ttatactttc atacaatact atttatcaaa gttcattaaa aaatctatca atagcctcgt
     6661 caacttcgga tcgagagatg gttttaacag tcgcttcttc tgctagagat gctactattt
     6721 gtttgccgta tcgttttccg atgattctcc tatccaaaat aagagttaag gaacgttggt
     6781 attcacgtct catacttctt cccaaagcct gttttaaacg aataatggcc attggcaatt
     6841 gataatcata aaaggcattt ttcccttctt gactcagttc ttgattaatc tttttcgtca
     6901 agggttcttg aggattttgg aaaggaagcc tcggtacaac ttgaatcaca aaaggatggc
     6961 ttgaaaaatc aactccctcc cagaaacttg ctgcaccaag caagatttgt tgttcacctt
     7021 tttcaaagcg tttctttagc tgatgaacat ccccattttt atactgggcc aagtggccaa
     7081 ctgtaagtaa atccgatact gctagaagca tgtctttagc ggtaaagaga accaaaatcg
     7141 gttgctggaa agcttgaatt tccactagta aagcagctac ctctctggca tagacttcta
     7201 aggaggtttc tgttaccaga gggaaatctt tgaccatgac cacttcttgt tcctgttttc
     7261 cccgagattc aatcttgaca aatttagctt caggatagcc taaaaggtct gccaaagaaa
     7321 ccctctgact aatctcaaga gtagccgata ctcccaagac ttggcaactc tcaggaagta
     7381 aagaggacag aagagtacgg cctgattttg tagaagaaat ctgaattttc ttttgactcg
     7441 tttcagttac ttcaagccag taatcacctt cagctgtaaa atagcgaacc agctcatcaa
     7501 agtcttctac ttccaattct gaaaaatact gatggagatt gtccagagaa tctaagatat
     7561 ttttcctaga tttgccagac tgaaattgtt ctatcaagta gagacactca aagcgaatac
     7621 tttctagtat ccgttgttga accctgtttt cttctcctac taaagcctta tcaattaaat
     7681 cgataataga ttgtatatcg taggtctctt gaagcagatt ttctagagct aacaaaatct
     7741 tttggacttc atcaataatc agtaaacggt cactgacaaa ttcaggatta tcttcaagtc
     7801 tggttacgag ataggcatga ttagtcacta aaagcttgca agtctctgcc ctttcttgac
     7861 tacgtttcca aaaatcttcc gtcacaaata agctctggga tgataaattc ccatcatgac
     7921 gaaggtctgc tagaaaatgt tggtaacggt agagttgccc gatttcatcc aaatctcctg
     7981 tctctgtctc agtaagccag accaagactt gcattttaaa gcgtctaaat aagcgatttt
     8041 catcattttc ctgcaaggaa tgataaaagg catccaactt cagataattt tgtggtccct
     8101 ttaagctatg aatatctgta tggaacactt ccttgaggcg tttaccttct tcttccatga
     8161 tttgattttg aagaatcttt gtcggaacac taagaacaat ttgtcgctct ttggattgag
     8221 ataaagcggg taagagatag ccataggttt tcccaatccc tgtcggcgct tgaatcagag
     8281 agacaggttc atctttcaat agcaagccaa cctctttagc aaaactttct tgttcctccc
     8341 tcacttcaag gttcaacaga gaaatatttt tagaaaagtc ttgagatagt tttcgtggct
     8401 ccagagaagc ttccgttttc ttaaaatata gaccttgaac ttggaccaag tctggagaac
     8461 tcaggataga ttggttgcga taagtttcct caataaccag gtaggactca tataggagag
     8521 cgtcagccat ttccagcaag cgttccaaga gacctttagg aagctgggtc atctttttcc
     8581 gtaaaaaaag aagtaattct gctgtagctt gggcatctga aagggctgtg tgtgcgtgtt
     8641 taagaggaat tcctaattct cgacacaaaa tcggcaaact atatttttcc agttcaggga
     8701 aaaagacctg ggccaattcg accgtatcaa cacgagggtt tcttagctca tagccttcaa
     8761 aaaataaatt ttccgccaag agattagcat caaactgaac attatgggct acaaaaatcc
     8821 catcctccac caagtcaaat atttttctgg caacttgcga aaaatcaggt gcttgcgcca
     8881 gacgttggtc tgtcaatcct gtcagttctt tgatatgagc atccaagggt tcatgtggat
     8941 tgacatccgt cgtatagtga tcgacgattt ctccgtcctc aatcacgaca attcccactt
     9001 ggataatttt agccttacta cctgtgctag ttgcctctaa atccaccaca acatagcgat
     9061 taccaatcgt tttcattata aaaaattata ccaaaaaaag ggccatttgg cacctgcaaa
     9121 cataggataa ttttcaaact caagaacact acttttctta aaaatcccat cgaatagagc
     9181 attctagcct gctgattttt gtaaaatagt gaagaaatac gaaaaattga gagaagaaat
     9241 gtttgcatct tccttctctc aacgaatcat gtattttatc taaccatgtc aggatcgtag
     9301 tcataaaatc ctgtatcaag gaaacggttt ttagggtgaa gtttacgaac ttcttcatcc
     9361 agcaagaagg cttgagcatg actaaagttg ccctcttgaa tcaagctacg cgcttggata
     9421 aagagttttg caatctcaac aaagttctga ccaggagtgt agagcccttc tagtttccag
     9481 cgagtaaagc catgctccac caattctgtt aatttgatca tcaaatcaag gtcattgttg
     9541 gcaaagatat gggtcccatg attatcttca aaaatggaat agtggctctc tggatcactt
     9601 ggctcagcca agaagaggtc acgtttatgc gtcttttcat catcgatatg tgtaaagtta
     9661 tagtagtttt gcaagagtgg acgtttagaa tgatggatga cgctagcacc gtaaaccaaa
     9721 acttcagcag gaatttccaa aatctctggc attttgaaaa gttcagctga tggaatttca
     9781 cgcgccaaaa cagcctcaga tgcgccagcc ttttgtcccc agaagttaat ctgacgactg
     9841 ctagttacca tggttgaagc atcgtagatg gtcttaaatg aataaccatc gcggttaact
     9901 acgtaaaaga cgcctgcatc cccaatcgta atatagtctg tcttgatttc ttccaagaag
     9961 tctaagaaag gcttgatacg gtccatcata tcttggtgca tgagagcatt gaccgcaacg
    10021 atcaattcct taccagcatc atgaaccaac ttagcgattt cacgtaattg gtcataacta
    10081 aaggtcgttg gcagacgaag accaaaatct ttctcaccga catagatacg gtctacgcca
    10141 gcttcgagta gttgttcaac ttgttcaata ctttcagcag ttgctgtaat gacaatcttt
    10201 tccataagaa aaattatacc acatttctcc aaaatcagcc atgctttgaa aatgaaaagg
    10261 gactttattc tttttgtaac ctttctgtaa taattctctg ccgaaaaaat gataaaatag
    10321 gtatgtactg ttaaggagag aatcatgccc gtaagaaaat tacaatccta tgaggtagac
    10381 tatcaagaag aattaaacca gcagcttcct cattaccaag cttatacacc tgaagcgcaa
    10441 tctgatgcca atctcaagga aatcctattt tttattaata tcgctgtttt ttgtatctgt
    10501 attgctatct ttagttttat ctttttagca ttaaaattat caactgctct tgcctttgcc
    10561 gcagcaatcg gattcagctt acttgtttta aaagttcaac ggtctattat caaacgaaaa
    10621 cgtagaagat aaatccaaaa tatcgcccca attggagcga tatttttatt ttttcttttt
    10681 agatggtgtc tgaatggcaa tcttcacttc aaagattgtt cccttgggtt tattatcttt
    10741 gacagtaaca gttcctttta gagcatctac aatttgcttg gctagggata atcctaaacc
    10801 aaaaccacct ttttgccggg ttctagcctt gtctactcga taaaaacggt caaaaatttt
    10861 ctttttatct tctgtcgaaa taccgattcc attatcagaa acaagtaaat aaagattgcg
    10921 atcggtcgcc gagataagaa aatcaatttc accatcctcc tcagtatact tgacggcatt
    10981 atcgaaaaga atggtcatca gttgtttcag aagaagctga tctgtgacaa ttgttcgatg
    11041 gatacgattt tcaaaacgga agacacgatt attttccgaa gcaatcatct catagtttgt
    11101 gaaagttgta ttaaaaaagc tagttggaac ttctgcaagc tccggcttaa tcccatcatc
    11161 tctccgagct aagttcagca agcttgtcgt taaaaaacgc atatttcgga cttcttccaa
    11221 actcgatgca atgctttcgc tcacatccat aatggtagct tctggcttac gaaaaagggt
    11281 ctctaagcga ttttgcaaaa ctgcgagtgg agttcgtaac tcatgactgg cattttccac
    11341 aaaagactgt tgcttctgca tactctcaag caggggccta acactgaccc tagctagata
    11401 gagactggca agtaaagaca aaatccagaa actagccatc acgaccacaa tcaattgctc
    11461 atgcttttga ctagcctgct ccaactgact ggtattaatc aagacagcag catacttgat
    11521 attggttgaa accgaactaa tattggtctc catcaaaatc acacgataga tttcttcctg
    11581 cccatagcta ttaaaaacct gaatctggta gatatgtcct agttctttct tttctaactt
    11641 aatcttatcc aagcccaaaa atcgatttcc agaaagaagt tgagtaaagt ctttatcaaa
    11701 gagaatgact tccgtattgg aactgacatt aggttttatt tctactttac tagcgtccgc
    11761 cctagcattt tctaaatctt taatctcttc tgttgcccta tttatagcca gctgaataac
    11821 tgcttgagga ttttcactca gtccatgaag cttatcgtcc accgaagtat aaagactcga
    11881 atgcatgact tgtaaaataa tcagagtcat tgtagaaaaa atcagggtga agacaccgaa
    11941 gttgcggata aaataactaa agtcatccgc ataccatgtt tttttaagtt tactgaacat
    12001 cttttaaaag atacccaaca ctacgcaagg tttgcaaatt ctctgcaaaa gtggttccct
    12061 ttaatttctt acggactttt gaaacataga cttcgacaac cgaaatcgtt gtatcactat
    12121 caaatcccca tagacggtca aaaatctgcg tcttaggcaa aatcacattt tgattttgaa
    12181 ggaaataaac tagtaaatcg aactctttcc ccagcaattc gacaggagta tcttcaactt
    12241 taacggtatt ggttgataaa ttaaccacga tattcccata agtcaaggtg ttttcattaa
    12301 acttccctga acgtttgaga agggcctgaa ttcgcatttt aagttcttct aggtagaaag
    12361 gtttggtcag ataatcatcc gctcccagtt caaatccatg tcccttgtca tccaaacttt
    12421 ccttggcagt cataatcaga actggtgtcg taattccctt ttcacgcaat tcttttaaga
    12481 cttggaaacc atttttttct ggcaacatca aatccagcaa aatcaagtca tagacaccac
    12541 tctcagcttc gtagagacct tcttctccat caaatacctg cataacatcc gcaaaatcgt
    12601 ctaaaaagtc aaatactgaa tttgacagac ctaggtcatc ctcaaccaat aagattttta
    12661 tcatgagaaa ctcctcctta ttaaaactat tataccaaat ttgccttaaa aaaaactcaa
    12721 ctctctgcat tttacatgag atagctgagt tttctttggc tctataatat ttgtagtggg
    12781 taaatcccct atggatatta tggagcttat ttttgtgtag aaaaaaagtc ccatatgacc
    12841 tataatgaaa agcgacaaaa caactcatta gaaaga
//
LOCUS       DAVKLW010000056        12584 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKLW010000056 DAVKLW010000000
VERSION     DAVKLW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.056"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..1113
                     /gene="dnaN"
                     /locus_tag="VW324_001668"
     CDS             <1..1113
                     /gene="dnaN"
                     /locus_tag="VW324_001668"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9727251.1"
                     /translation="NLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGITLIGSNGQI
                     SIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGK
                     SEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFV
                     LSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFF
                     ANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSA
                     TQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALN
                     SEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1178..1372
                     /locus_tag="VW324_001669"
     CDS             1178..1372
                     /locus_tag="VW324_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9727252.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1456..2571
                     /gene="ychF"
                     /locus_tag="VW324_001670"
     CDS             1456..2571
                     /gene="ychF"
                     /locus_tag="VW324_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9727253.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2643..3212
                     /locus_tag="VW324_001671"
     CDS             2643..3212
                     /locus_tag="VW324_001671"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9727254.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            3213..6722
                     /gene="mfd"
                     /locus_tag="VW324_001672"
     CDS             3213..6722
                     /gene="mfd"
                     /locus_tag="VW324_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9727255.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6780..7046
                     /locus_tag="VW324_001673"
     CDS             6780..7046
                     /locus_tag="VW324_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9727256.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7039..7407
                     /locus_tag="VW324_001674"
     CDS             7039..7407
                     /locus_tag="VW324_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9727257.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7527..8795
                     /locus_tag="VW324_001675"
     CDS             7527..8795
                     /locus_tag="VW324_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9727258.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8792..10069
                     /gene="tilS"
                     /locus_tag="VW324_001676"
     CDS             8792..10069
                     /gene="tilS"
                     /locus_tag="VW324_001676"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9727259.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIINTVLYIEKIDR"
     gene            10073..10615
                     /gene="hpt"
                     /locus_tag="VW324_001677"
     CDS             10073..10615
                     /gene="hpt"
                     /locus_tag="VW324_001677"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9727260.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10631..>12584
                     /gene="ftsH"
                     /locus_tag="VW324_001678"
     CDS             10631..>12584
                     /gene="ftsH"
                     /locus_tag="VW324_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9727261.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4329 a         1896 c         2562 g         3797 t
ORIGIN      
        1 aatttatttc tacaagcatt aaatactact aagagagcta ttagttctaa aaatgccatt
       61 cctattttat caacagtaaa aattgacgtg accaatgaag gtattacttt aattggttca
      121 aatggtcaaa tttcaattga aaattttatt tctcaaaaaa atgaagatgc tggtttgtta
      181 attacttctt taggttcgat ccttcttgaa gcttctttct ttatcaatgt agtatctagt
      241 ttacctgatg tgactcttga ttttaaagaa attgaacaaa atcaaattgt tttaaccagt
      301 ggcaaatcag aaattaccct aaaaggaaaa gatagcgaac aatatccacg aatccaagaa
      361 atttcagcaa gcactccttt aatacttgaa acaaaattac tcaagaaaat tattaatgaa
      421 acagcctttg ctgcaagtac acaagagagt cgtccgattt taacaggtgt ccacttcgta
      481 ttgagtcaac acaaagagtt aaaaacagtt gcaacagact ctcatcgcct aagccagaaa
      541 aaattgactc ttgaaaaaaa tagtgatgat tttgatgtcg taattcctag ccgttctcta
      601 cgcgaatttt cagcggtatt tacagatgat atcgaaactg tagagatttt ctttgccaat
      661 aaccaaatcc tctttagaag cgaaaatatt agcttctata ctcgtctcct agaaggaaac
      721 tatcctgata cagatcgctt gattccaaca gactttaaca ctactattac ttttaatgtg
      781 gtaaacttac gccagtcaat ggagcgtgcc cgtcttttat caagtgcgac tcaaaatggt
      841 actgtgaaac ttgaaattaa ggatggggtt gttagcgccc atgttcactc tccagaagtt
      901 ggtaaagtaa acgaagaaat cgatactgat caggttactg gtgaagattt gaccattagt
      961 ttcaacccaa cttacttgat tgattctctt aaagctttaa atagcgaaaa ggtgaccatt
     1021 agctttatct cagctgttcg tccatttact cttgtgccag cagatactga cgaagacttc
     1081 atgcagctca ttacaccagt tcgtacaaat taagtgaaag aggttgagcc tggctcgcct
     1141 cttttatgat ataatcgaaa aagaaaagga gagtagtatg tatcaagttg gaaattttgt
     1201 tgagatgaaa aaatcacacg cttgtacaat caagtcgact ggtaaaaagg ctaatcgttg
     1261 ggaaattaca cgtgtaggag cagatatcaa aataaaatgc agtaattgtg agcatgttgt
     1321 catgatgggg cgatatgatt ttgagcgaaa aatgaataaa attattgact gagaaccctt
     1381 agttagaggg ttagcacttt atcccttttt gtgttataat attagggatt gaaatgaaaa
     1441 cggagaatga gaaatatggc tttgacagca ggtatcgttg gtttgccaaa cgttggtaaa
     1501 tcaacattat ttaatgcaat tacaaaagca ggagcagagg cagcaaacta cccatttgcg
     1561 actattgatc caaatgttgg aatggtggaa gttccagatg aacgcctaca aaaactaact
     1621 gaaatgataa ctcctaaaaa gacagttccc acaacatttg aatttacaga tattgcaggg
     1681 attgtaaaag gagcttcaaa aggagaaggg ctagggaata aattcttggc caatattcgt
     1741 gaagtagatg cgattgttca cgtagttcgt gcttttgatg atgaaaatgt gatgcgcgag
     1801 caaggacgtg aagacgcctt tgtagatcca cttgcagata ttgatacaat taatctggaa
     1861 ttgattcttg ctgacttaga atcagtgaac aaacgatatg cgcgtgtaga aaagatggca
     1921 cgtacgcaaa aagataaaga atcagtagca gaattcaatg ttcttcaaaa gattaaacca
     1981 gtcctagaag acgggaaatc agctcgtacc attgaattta cagatgagga acaaaaggtt
     2041 gtcaaaggtc ttttcctttt gacgactaaa ccagttcttt atgtagctaa tgtggacgag
     2101 gatgtggttt cagaacctga ctctatcgac tatgtcaaac aaattcgtga atttgcagcg
     2161 acagaaaatg ctgaagtagt cgttatttct gcgcgtgctg aggaagaaat ttctgaattg
     2221 gatgatgaag ataaaaaaga gtttcttgaa gccattggtt tgacagaatc aggtgtagat
     2281 aagttgacgc gtgcagctta ccacttgctt ggattgggaa cttacttcac agctggtgaa
     2341 aaagaagttc gcgcttggac tttcaaacgt ggtatgaagg ctcctcaagc agctggtatt
     2401 atccactcag actttgaaaa aggctttatt cgtgcagtaa ccatgtcata tgaagatcta
     2461 gtgaaatacg gatctgaaaa ggccgtaaaa gaagctggac gcttgcgtga agaaggaaaa
     2521 gaatatatcg ttcaagatgg cgatatcatg gaattccgct ttaatgtcta aaaattaata
     2581 aatggtgtca attaggttgg aaaaaaaatt ccaacccttt tggcttttga aaggaaaaat
     2641 aaatgaccaa attacttgta ggcttgggaa atccagggga taaatatttt gaaacaaaac
     2701 acaatgttgg ttttatgttg attgatcaac tagcgaagaa acagaatgtc acttttacac
     2761 acgataagat atttcaagct gacctagcat cctttttcct aaatggagaa aaaatttatc
     2821 tggttaaacc aacgaccttt atgaatgaaa gtggaaaagc agttcatgct ttattaactt
     2881 actatggttt ggatattgac gatttactta tcatttacga tgatcttgac atggaagttg
     2941 ggaaaattcg tttaagagca aaaggctcag caggtggtca taatggtatc aagtctatta
     3001 ttcaacatat aggaactcag gtctttaacc gtgttaagat tggaattgga agacctaaaa
     3061 atggtatgtc agttgttcat catgttttga gtaagtttga cagggatgat tatatcggta
     3121 ttttacagtc tgttgacaaa gttgacgatt ctgtaaaata ctatttacaa gagaaaaatt
     3181 ttgagaaaac aatgcagagg tataacggat aaatggtgac cttattagat ttattctcag
     3241 aaaatgatca gattaaaaaa tggcatcaaa atttaacaga taagaaaaga caactaatac
     3301 ttggtttatc aacatctact aaggctcttg caattgcaag cagtttagaa aaagaagata
     3361 ggattgtgtt attgacgtca acttatggag aagcagaagg acttgttagt gatcttattt
     3421 ctatcttggg tgaggaactc gtctatccat ttttggtaga tgatgctcct atggtggagt
     3481 ttttgatgtc ttcacaggaa aaaattattt cacgggttga agccttgcgt tttttgactg
     3541 attcatctaa gaaagggatt ttagtttgta atatcgcagc aagtcgattg attttaccgt
     3601 ctcccaatgc attcaaagat agtattgtaa aaatctcagt tggtgaagaa tatgatcaac
     3661 acgcgtttat ccatcagtta aaggaaaatg gctatcgaaa agttactcaa gtacaaactc
     3721 agggcgaatt tagtcttcga ggagatattt tagatatttt tgaaatatcc cagttagaac
     3781 cttgtcgaat tgagtttttt ggtgatgaaa ttgatggtat caggtcattt gaagtagaaa
     3841 cacaattatc gaaagaaaat aagacagaac tcactatctt tccagctagt gatatgcttt
     3901 tgagagaaaa ggattatcaa cgaggacagt cagctttaga aaaacaaatt tcaaaaactt
     3961 tatcacctat tttgaaatca tacctagaag aaattctttc aagttttcac caaaaacaaa
     4021 gtcatgcaga ctctcggaag tttttatctt tgtgctatga taagacatgg actgtctttg
     4081 attatattga aaaagatact ccaatattct ttgatgatta tcaaaaattg atgaatcagt
     4141 atgaagtctt tgaaagagac ttagcgcagt actttacaga agaattacag aatagtaaag
     4201 cattttctga tatgcagtat ttttctgata ttgaacaaat ctataaaaaa caaagtccag
     4261 tgaccttttt ctctaatctt caaaagggtt taggaaatct caaatttgac aaaatttatc
     4321 aatttaatca atatcctatg caggaatttt tcaatcagtt ttcttttcta aaagaagaaa
     4381 ttgaacgata taaaaaaatg gattacacca ttattctgca gtctagcaat tcaatgggaa
     4441 gtaaaacatt ggaggatatg ttagaggaat atcagattaa attggattct agagataaga
     4501 caagtatctg taaagaatct gtaaacttaa tagagggtaa tctcagacat ggttttcatt
     4561 ttgtagatga aaagatttta ttgataactg aacatgagat ttttcaaaag aaattaaagc
     4621 gtcgttttcg aagacaacat gtttcaaatg cagagagatt aaaagattac aatgaacttg
     4681 aaaaagggga ctatgttgtc catcatatcc atgggattgg tcaatatcta ggaattgaaa
     4741 ccattgaaat caagggaatt catcgcgatt atgtcagtgt ccaataccaa aatggtgatc
     4801 aaatttctat ccccgtggaa cagattcatc tactgtccaa atatatttca agtgatggta
     4861 aagctccaaa actcaataaa ttaaatgacg gtcattttaa aaaggccaag caaaaggtta
     4921 agaaccaggt agaggatata gctgatgatt taatcaaact ctactctgaa cgtagtcagt
     4981 tgaagggttt tgctttctca gctgatgatg atgatcaaga tgcctttgat gatgctttcc
     5041 cttatgttga aacggatgat caacttcgta gtattgagga aatcaagagg gatatgcagg
     5101 cttctcagcc aatggatcga cttttagttg gggatgttgg ttttggaaag actgaagttg
     5161 ctatgcgtgc agcctttaaa gcagtcaatg atcacaaaca ggttgtcatt ctagttccga
     5221 cgacggtttt agcgcaacag cactatacga attttaagga acgattccaa aattttgcag
     5281 ttaatgttga tgtgttgagt cgctttagaa gtaaaaaaga gcagactgca acacttgaaa
     5341 aattgaaaaa cggtcaagtc gatattttga ttggaacaca tcgtgttttg tcaaaagatg
     5401 ttgtgtttgc tgatttgggc ttgatgatta ttgatgagga acagcgattt ggtgtcaagc
     5461 ataaggaaac tttgaaagaa ctgaagaaac aagtggatgt cctaaccttg accgctacgc
     5521 caatccctcg taccctccat atgtctatgc tgggaatcag agatttatct gttattgaaa
     5581 ctccgccgac taatcgctat cctgttcaga cctatgtttt ggaaaagaat gatagtgtca
     5641 ttcgtgatgc tgtcttgcgt gaaatggagc gtggaggtca agtttattat ctttacaaca
     5701 aagttgacac aattgttcag aaggtttcag aattacagga gttgattccg gaggcttcga
     5761 ttggatatgt tcatggtcga atgagtgaag tccagttgga aaatactcta ttagacttta
     5821 ttgagggaca atacgatatc ttggtgacga ctactattat tgagacaggg gtggacattc
     5881 caaatgctaa tactttattt attgaaaatg cggaccatat gggcttgtca accttatatc
     5941 agttaagagg aagagtcggt cgtagtaatc gtattgctta tgcttatctc atgtatcgtc
     6001 cagaaaaatc aatcagtgaa gtctctgaaa agagattaga agcgattaaa ggatttacag
     6061 aattgggctc tggctttaag attgcaatgc gagatctttc gattcgtgga gcaggaaatc
     6121 ttttaggaaa atcccagtct ggtttcattg attctgttgg ttttgaattg tattcgcagt
     6181 tattagagga agctattgct aaacgaaacg gtaatgctaa cgctaacaca agaaccaaag
     6241 ggaatgctga gttgattttg caaattgatg cctatcttcc tgatacttat atttctgatc
     6301 aacgacataa gattgaaatt tacaagaaaa ttcgtcaaat tgacaaccgt gtcaattatg
     6361 aagagttaca agaggagttg atagaccgtt ttggagaata cccagatgta gtagcctatc
     6421 ttttagagat tggtttggtt aaatcatact tggacaaggt ctttgttcaa cgtgtggaaa
     6481 gaaaagataa taaaattaca attcaatttg aaaaagtcac tcaacggctg tttttagctc
     6541 aagattattt taaagcttta tccgtaacga acttaaaagc aggcatcgct gagaataagg
     6601 gattaatgga gcttgtattt gatgtccaaa ataagaaaga ttatgaaatt ttagaaggtc
     6661 tgctgatttt tggagaaagt ttattagaga taaaagagtt taaggaagaa aattccattt
     6721 gatatttttc ttctataaaa tagataaaaa tggtacaata ataaattgag gtaataagga
     6781 tgagattaga taaatattta aaagtatcgc gaattatcaa gcgtcgtaca gtcgcaaagg
     6841 aagtagcaga taaaggtaga atcaaggtta atggaatctt ggccaaaagt tcaacggact
     6901 tgaaagttaa tgaccaagtt gaaattcgct ttggcaataa gttgctgctt gtaaaagtac
     6961 tagagatgaa agatagtaca aaaaaagaag atgcagcagg aatgtatgaa attatcagtg
     7021 aaacacgggt agaagaaaat gtctaaaaat attgtacaat tgaataattc ttttattcaa
     7081 aatgaatacc aacgtcgtcg ctacctgatg aaagaacgac aaaaacggaa tcgttttatg
     7141 ggaggggtat tgattttgat tatgctatta tttatcttgc caacttttaa tttagcgcag
     7201 agttatcagc aattactcca aagacgtcag caattagcag acttgcaaac tcagtatcaa
     7261 actttgagtg atgaaaagga taaggagaca gcatttgcta ccaagttgaa agatgaagat
     7321 tatgctgcta aatatacacg agcgaagtac tattattcta agtcgaggga aaaagtttat
     7381 acgattcctg acttgcttca aaggtgataa aatggaaaat ttattagacg taatagagca
     7441 atttttgagt ttgtcagatg aaaagctgga agaattggct gataaaaatc aattattgcg
     7501 tttacaagaa gaaaaggaaa ggaagaatgc gtaaattctt aattattttg ttgctaccaa
     7561 gttttttgac catttcaaaa gtcgttagca cagaaaaaga agtcgtctat acttcgaaag
     7621 aaatttatta cctttcacaa tctgacttta gtatttattt tagagaaaaa ttaagttctc
     7681 ccatggttta tggagaggtt cctgtttatg cgaatgaaga tttagtagtg gaatctggga
     7741 aattgactcc caaaacaagt tttcaaataa ccgagtggcg cttaaataaa caaggaattc
     7801 cagtatttaa gctatcaaat catcaattta tagctgcgga caaacgattt ttatatgatc
     7861 aatcagaggt aactccaaca ataaaaaaag catggttaga atctgacttt aaactgtaca
     7921 atagtcctta tgatttaaaa gaagtgaaat catccttatc agcttattcg caagtatcaa
     7981 tcgacaagac catgtttgta gaaggaagag aatttctaca tattgatcag gctggatggg
     8041 tagctaaaga atcaacttct gaagaagata atcggatgag taaagttcaa gaaatgttat
     8101 ctgaaaaata tcagaaagat tctttctcta tttatgttaa gcaactgact actggaaaag
     8161 aagctggtat caatcaagat gaaaagatgt atgcagccag cgttttgaaa ctctcctatc
     8221 tctattatac gcaagaaaaa ataaatgagg gtctttatca gttagatacg actgtaaaat
     8281 acgtatctgc agtcaatgat tttccaggtt cttataaacc agagggaagt ggtagtcttc
     8341 ctaaaaaaga agataataaa gaatattctt taaaggattt aattacgaaa gtatcaaaag
     8401 aatctgataa tgtagctcat aatctattgg gatattacat ttcaaaccaa tctgatgcca
     8461 cattcaaatc caagatgtct gccattatgg gagatgattg ggatccaaaa gaaaaattga
     8521 tttcttctaa gatggccggg aagtttatgg aagctattta taatcaaaat ggatttgtgc
     8581 tagagtcttt gactaaaaca gattttgata gtcagcgaat tgccaaaggt gtttctgtta
     8641 aagtagctca taaaattgga gatgcggatg aatttaagca tgatacgggt gttgtctatg
     8701 cagattctcc atttattctt tctattttca ctaagaattc tgattatgat acgatttcta
     8761 agatagccaa ggatgtttat gaggttctaa aatgagggaa ccagattttt taaatcattt
     8821 tctcaagaag ggatatttca aaaagcatgc taaggcggtt ctagctcttt ctggtggatt
     8881 agattccatg tttctattta aggtattgtc tacttatcaa aaagagttag agattgaatt
     8941 gattctagct catgtgaatc ataagcagag aattgaatca gattgggaag aaaaggaatt
     9001 aaggaagttg gctgctgaag cagagcttcc tatttatatc agcaattttt caggagaatt
     9061 ttcagaagcg cgtgcacgaa attttcgtta tgattttttt caagaggtta tgaaaaagac
     9121 aggtgcgaca gctttagtca ctgcccacca tgctgatgat caggtggaaa cgattttgat
     9181 gcgcttgatt cgaggaactc gcttgcgcta tctatcagga attaaggaga agcaagtagt
     9241 cggagagata gaaatcattc gtcccttctt gcattttcag aaaaaagact ttccatcaat
     9301 ttttcacttt gaagatacat caaatcagga gaatcattat tttcgaaatc gtattcgaaa
     9361 ctcttactta ccagaattgg aaaaagaaaa tcctcgattt agggatgcaa tcttaggcat
     9421 tggcaatgaa attttagatt atgatttggc aatagctgaa ttatctaaca atattaatgt
     9481 ggaagattta cagcagttat tttcttactc tgagtctaca caaagagttt tacttcaaac
     9541 ttatctgaat cgttttccag atttgaatct tacaaaagct cagtttgctg aagttcagca
     9601 gattttaaaa tctaaaagcc agtatcgtca tccgattaaa aatggctatg aattgataaa
     9661 agagtaccaa cagtttcaga tttgtaaaat cagtccgcag gctgatgaaa aggaagatga
     9721 acttgtgtta cactatcaaa atcaggtagc ttatcaagga tatttatttt cctttggact
     9781 tccattagaa ggtgaattaa ttcaacaaat acctgtttca cgtgaaacat ccatacacat
     9841 tcgtcatcga aaaacaggag atgttttgat tcaaaatggg catagaaaaa aactcagacg
     9901 tttatttatt gatttgaaaa tccctatgga aaagagaaac tctgctctta ttattgagca
     9961 atttggtgaa attgtctcaa ttttgggaat tgcgaccaat aatttgagta aaaaaacgaa
    10021 aaatgatata attaacactg tactttatat agaaaaaata gataggtaaa aaatgttaga
    10081 aaacgatatt aaaaaagtcc tcgtttcaca cgatgaaatt acagaagcag ctaaaaaact
    10141 aggtgctcaa ttaactaaag actatgcagg aaaaaatcca atcttagttg ggattttaaa
    10201 aggatctatt ccttttatgg ctgaattggt caaacatatt gatacacata ttgaaatgga
    10261 cttcatgatg gtttctagct accatggtgg aacagcaagt agtggtgtta tcaatattaa
    10321 acaagatgtg actcaagata tcaaaggaag acatgttcta tttgtagaag atatcattga
    10381 tacaggtcaa actttgaaga atttgcgaga tatgtttaaa gaaagagaag cagcttctgt
    10441 taaaattgca accttgttgg ataaaccaga aggacgtgtt gtagaaattg aggcagacta
    10501 tacctgcttt actatcccaa atgagtttgt agtaggttat ggtttagact acaaagaaaa
    10561 ttatcgtaat cttccttata ttggagtatt gaaagaggaa gtgtattcaa attagaaaga
    10621 ataatcttta atgaaaaaac aaaataatgg tttaattaaa aatccttttc tatggttatt
    10681 atttatcttt ttccttgtga caggattcca gtatttctat tctgggaata actcaggagg
    10741 aagtcagcaa atcaactata ctgagttggt acaagaaatt accgatggta atgtaaaaga
    10801 attaacttac caaccaaatg gtagtgttat cgaagtttct ggtgtctata aaaatcctaa
    10861 aacaagtaaa gaaggaacag gtattcagtt tttcacgcca tctgttacta aggtagagaa
    10921 atttaccagc actattcttc ctgcagatac taccgtatca gaattgcaaa aacttgctac
    10981 tgaccataaa gcagaagtaa ctgttaagca tgaaagttca agtggtatat ggattaatct
    11041 actcgtatcc attgtgccat ttggaattct attcttcttc ctattctcta tgatgggaaa
    11101 tatgggagga ggcaatggcc gtaatccaat gagttttgga cgtagtaagg ctaaagcagc
    11161 aaataaagaa gatattaaag taagattttc agatgttgct ggagctgaag aagaaaaaca
    11221 agaactagtt gaagttgttg agttcttaaa agatccaaaa cgattcacaa aacttggagc
    11281 ccgtattcca gcaggtgttc ttttggaggg acctccgggg acaggtaaaa ctttgcttgc
    11341 taaggcagtc gctggagaag caggtgttcc attctttagt atctcaggtt ctgactttgt
    11401 agaaatgttt gtcggagttg gagctagtcg tgttcgctct ctttttgagg atgccaaaaa
    11461 agcagcacca gctatcatct ttatcgatga aattgatgct gttggacgtc aacgtggagt
    11521 cggtctcggc ggaggtaatg acgaacgtga acaaaccttg aaccaacttt tgattgagat
    11581 ggatggtttt gagggaaatg aagggattat cgtcatcgct gcgacaaacc gttcagatgt
    11641 acttgatcct gcccttttgc gtccaggacg ttttgataga aaagtattgg ttggccgtcc
    11701 tgatgttaaa ggtcgtgaag caatcttgaa agttcacgct aagaacaagc ctttagcaga
    11761 agatgttgat ttgaaattag tggctcaaca aactccaggc tttgttggtg ctgatttaga
    11821 gaatgtcttg aatgaagcag ctttagttgc tgctcgtcgc aataaatcga taattgatgc
    11881 ttcagatatt gatgaagcag aagatagagt tattgctgga ccttctaaga aagataagac
    11941 agtttcacaa aaagaacgag aattggttgc ttaccatgag gcaggacata ccattgttgg
    12001 tctagtcttg tcgaatgctc gcgttgtcca taaggttaca attgtaccac gcggccgtgc
    12061 aggcggatac atgattgcac ttcctaaaga ggatcaaatg cttctatcta aagaagatat
    12121 gaaagagcaa ttggctggct taatgggtgg acgtgtagct gaagaaatta tctttaatgt
    12181 ccaaactaca ggagcttcaa acgactttga acaagcgaca caaatggcac gtgcaatggt
    12241 tacagagtac ggtatgagtg aaaaacttgg cccagtacaa tatgaaggaa accatgctat
    12301 gtttggtgca cagaatcctc aaaaatcaat ttcagaacaa acagcttatg aaattgatga
    12361 agaggttcgt tcattattaa atgaggcacg aaataaagct gctgaaatta ttcagtcaaa
    12421 tcgtgaaact cacaagttaa ttgcagaagc attattgaaa tacgaaacat tggatagtac
    12481 acaaattaaa gctctttacg aaacaggaaa gatgcctgaa gcagtagaag aggaatctca
    12541 tgcactatcc tatgatgaag taaagtcaaa aatgaatgac gaaa
//
LOCUS       DAVKLW010000057        12302 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKLW010000057 DAVKLW010000000
VERSION     DAVKLW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.057"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            89..3190
                     /locus_tag="VW324_001679"
     CDS             89..3190
                     /locus_tag="VW324_001679"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9727262.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLIGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3273..4280
                     /gene="pfkA"
                     /locus_tag="VW324_001680"
     CDS             3273..4280
                     /gene="pfkA"
                     /locus_tag="VW324_001680"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9727263.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4339..5844
                     /gene="pyk"
                     /locus_tag="VW324_001681"
     CDS             4339..5844
                     /gene="pyk"
                     /locus_tag="VW324_001681"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9727264.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6579..6804)
                     /locus_tag="VW324_001682"
                     /pseudo
     CDS             complement(6579..6804)
                     /locus_tag="VW324_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7652..8524
                     /locus_tag="VW324_001683"
     CDS             7652..8524
                     /locus_tag="VW324_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9727265.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8670..9014)
                     /locus_tag="VW324_001684"
                     /pseudo
     CDS             complement(8670..9014)
                     /locus_tag="VW324_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9157..9468
                     /locus_tag="VW324_001685"
     CDS             9157..9468
                     /locus_tag="VW324_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727266.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9472..10188
                     /locus_tag="VW324_001686"
     CDS             9472..10188
                     /locus_tag="VW324_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9727267.1"
                     /translation="MKKYFIGGLGNNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYLTSEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLALLISKEKSGAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10318..11133)
                     /locus_tag="VW324_001687"
     CDS             complement(10318..11133)
                     /locus_tag="VW324_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9727268.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11130..12035)
                     /locus_tag="VW324_001688"
     CDS             complement(11130..12035)
                     /locus_tag="VW324_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9727269.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
BASE COUNT         3849 a         2152 c         2610 g         3691 t
ORIGIN      
        1 tagtaagctg atgaaatttt tctcaagaga agccatttat aggtgaaaat ggtataatat
       61 agtgagaagg atagaggaga agtgtaaatt gatcgcacaa ctagatacaa aaacagtcta
      121 tagttttatg gaaagcgtca tttcgatcga aaagtatgtg agagcagcta aagaatacgg
      181 ctacactcat ttggctatga tggatattga caatctttat ggcgctttcg actttctaga
      241 gattacaaaa aaatacggca ttcatccttt gatagggctt gaaatgacag tgtttgtaga
      301 tgatcaggaa gtgaatttgc gctttttagc tctatctagt gtgggctatc agcagttgat
      361 gaagctttcg acagccaaga tgcaggggga gaaaacttgg tcagtcctgt cccagtacct
      421 ggaggatatc gcggtcattg tgccttattt tgatagagtt gagtcgttag aactaggctg
      481 tgattactat ataggggttt atccagaaac actagcaagc gaatttcatc atcctatctt
      541 acctctttat cgggtcaacg cttttgaaag cagggataga gaagttcttc aagttttaac
      601 agcgattaaa gaaaatctac cgctcagaga agttcccttg cgttcgagac aagatgtctt
      661 tatatcagca agttctttag agaaactatt ccaagagtgt tttccgcaag ctttggacaa
      721 tttagaaaag cttatttcag gcatttctta cgacttggat actagtctga aactgcctcg
      781 ttttaatcca gctagaccag cagtagagga gttgagagag cgtgctgaac tggggcttgt
      841 tcagaagggg ttgactagta aagaatatca agatagacta gaccaagaat tgtctgttat
      901 tcatgatatg ggctttgatg attatttctt ggttgtttgg gatttgttgc gttttggacg
      961 atcgaatggc tattatatgg gaatgggaag gggttctgca gtaggcagtt tggtttctta
     1021 tgccttagac atcacgggga ttgacccagt agagaaaaat ctgatttttg aacgctttct
     1081 taatcgtgaa cgctatacca tgcctgatat tgatattgat atcccagata tttatcgtcc
     1141 agattttatc agatatgttg gtaataaata tggtagtaaa catgcggcac aaatcgttac
     1201 tttttcaacc tttggagcca agcaagctct tcgagatgtc ttgaaacgct ttggtgtgcc
     1261 agagtatgaa ttatctgcaa ttactaagaa aatcagtttt cgtgacaatc ttaagtcggc
     1321 ctatgagggc aatctccagt ttcgtcagca aatcaatagt aagttagaat accaaaaagc
     1381 ttttgagatt gcttgcaaga tagagggcta tccaagacaa acctctgtcc atgcggctgg
     1441 tgttgtaatt agtgaccaag atttaaccaa ctatattcct ctaaagtatg gtgatgaaat
     1501 tccactgact cagtatgatg ctcatggagt tgaggctagc ggacttttga agatggactt
     1561 tctgggacta cgaaatttga cctttgtcca gaagatgcaa gagttgcttg ctgaaataga
     1621 aggtattcac cttaaaattg aagaaataga tttggaagac aaagaaacgt tagctttatt
     1681 tgcctctgga aatacaaaag gtatctttca atttgagcaa ccaggtgcca ttcgtctgct
     1741 taagcgtgtg caaccagtct gttttgaaga tgtcgtcgcg actacttctc taaatcgacc
     1801 gggtgctagt gactatatca ataattttgt ggcaagaaag catgggcagg aagaagtgac
     1861 tgttctggat ccagtactgg aggatatttt ggctccaacc tacggcataa tgctctatca
     1921 ggagcaggtt atgcaggttg cccagcgatt tgccggattt agtcttggga aagccgatat
     1981 tttgcgtcgg gctatgggga aaaaggatgc ctctgccatg catgagatga gggcttcctt
     2041 tattcaaggt tcattagaag ctggtcatac tgtggaaaaa gcagagcagg tctttgatgt
     2101 tatggagaag tttgcaggtt atggttttaa caggtcacac gcctatgcct actcagcctt
     2161 ggccttccag ttggcttatt ttaaaacaca ttatccagcc attttttatc aggtcatgtt
     2221 aaattcttcc aacagtgatt acttaataga tgcacttgaa gcaggttttg aagtagcctc
     2281 tctatccatc aacaccattc cctatcacga taaaattgcc aacaaggcca tctatctagg
     2341 tttgaaatcc attaaaggag tcagtaatga tttagctctc tggattattg aaaatagacc
     2401 ttattctaac attgaagatt ttatagctaa attacctgag aattatctga aacttcctct
     2461 gctagaacct ttggtaaaag ttggtctttt cgattcattt gaaaaaaatc gtcaaaaagt
     2521 atttaataat ttagctaatc tatttgaatt tgtgaaagag ttgggaagtt tgtttggaga
     2581 tgctatttat agttggcagg aatcggaaga ttggacggaa caagaaaaat tttatatgga
     2641 acaagagctt ttagggatag gtgtcagcaa acatccacta caagctattg caagtaaggc
     2701 tatttacccg attaccccaa tcggaaattt gtcagaaaat agctatgcta ttatcttggt
     2761 tgaagttcag aaaataaaag tgattcgtac caaaaagggt gaaaatatgg ccttcttaca
     2821 ggcagatgat agtaagaaaa aattggatgt cactctcttt tcagacttat atcgtcaggt
     2881 tggacaggaa ataaaagagg gagccttcta ctatgtaaaa ggaaaaatac aatcacgtga
     2941 tggccgtctg caaatgattg cacaagaaat aagagaagca gttgctgaac gcttttggat
     3001 acaggtgaaa aatcatgaat cggatcaaga aatttcacgc attttagaac aatttaaagg
     3061 cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa accatcgttt ctccccatca
     3121 ttttgtagct aaatccaatg aattagagga gaaattgaat gaaatcgtta tgaaaacgat
     3181 ttatcgctaa aaatacggaa aatagaagaa ttttaaaatc aaatgtgata taatcaagaa
     3241 gaatgttaaa agaaaaagga gcataaacca atatgaaacg tattgctgtt ttgactagtg
     3301 gcggagacgc acctggtatg aacgctgcca tccgtgcagt tgttcgtcaa gcaatttcag
     3361 aaggaatgga agtgtttggt atctatgacg gatatgctgg tatggttgcc ggtgaaattc
     3421 atcccctaga tgcagcttca gtaggggaca tcatttctcg tggtggtact tttcttcact
     3481 cagctcgtta tccagagttc gctcaacttg aagggcaact taaagggatt gagcaattga
     3541 aaaaacacgg aattgaaggt gtagttgtta tcggtggtga cggatcttac cacggcgcta
     3601 tgcgtttgac tgaacatggc ttcccagcta ttggtcttcc aggtacaatc gataacgata
     3661 tcgttggtac tgactttaca atcggttttg acacagcggt tactactgcc atggacgcta
     3721 tcgataagat tcgtgataca tcatcaagtc accgtcgtac ttttgtaatc gaagttatgg
     3781 gacgtaacgc tggtgatatc gctctttggg ctggtattgc aactggtgct gatgaaatca
     3841 tcatccctga agcaggcttc aagatggaag atatcgtagc aagcatcaaa gctggttatg
     3901 aatgtggtaa aaaacacaat atcatcgtct tagctgaagg tgtgatgtca gcggctgaat
     3961 ttggtcaaaa acttaaagaa gctggagata caagcgacct tcgtgtaaca gaacttggac
     4021 atattcaacg tggtggttct ccaactgcgc gtgaccgtgt tttggcgtca cgtatgggtg
     4081 cacatgctgt taaacttctt aaagaaggta tcggtggtgt tgcggttggt attcgtaacg
     4141 aaaaaatggt tgaaaatcca attcttggta ctgcagaaga aggggcattg tttagcctta
     4201 ctgcagaagg taagattgtg gttaacaacc cgcacaaagc cgatattgag ctatctagct
     4261 tgaataagag cttgtcataa tttacaattt agttaataag accaaaaagg tcatatatat
     4321 aaaggagtca caaaaatcat gaacaaacgt gtaaagatcg ttgcaacttt gggtcctgcg
     4381 gtagaaatcc gtggtggtaa aaaattcggt gaggacggat actggggtga aaaacttgat
     4441 gttgaagctt cagctaaaaa cattgctaaa ttgattgaag ctggtgctaa cacattccga
     4501 ttcaacttct cacacggcga ccaccaagaa caaggtgagc gtatggcaac tgttaaactt
     4561 gcggaaaaaa ttgcaggtaa aaaagttggt ttccttcttg atacaaaagg acctgaaatc
     4621 cgtacagaat tgttcgaagg tgaagctaaa gaatattcat acaaaactgg tgaaaaaatt
     4681 cgtgttgcaa ctaaacaagg aatcaaatca actcgtgaag tgattgcgtt gaacgttgct
     4741 ggtgctcttg atatctatga tgatgttgaa gttggtcgtc aagttttggt tgacgatggt
     4801 aaacttggtc ttcgtgtggt tgctaaagat gatgcaactc gtgaatttga agttgaagtt
     4861 gaaaacgatg gtatcatcgc taaacaaaaa ggtgtgaaca tccctaacac taaaattcct
     4921 ttcccagctc ttgctgaacg cgataacgac gatatccgtt tcggtcttga acaaggtatc
     4981 aacttcatcg caatttcatt cgtacgtact gcaaaagatg tgaacgaagt tcgtgcaatc
     5041 tgtgaagaaa ctggaaacgg acatgttcaa ttgttcgcta aaatcgaaaa ccaacaaggt
     5101 atcgataact tagatgaaat catcgaagca gctgatggta ttatgattgc tcgtggtgat
     5161 atgggtatcg aagtaccgtt cgaaatggtt ccagtttatc aaaaaatgat tatcaagaaa
     5221 gtcaatgctg caggtaaagt tgttatcact gcaacaaaca tgcttgaaac aatgactgaa
     5281 aaaccacgtg caactcgttc agaagtatca gacgtattca acgctgttat cgacggaact
     5341 gacgctacaa tgttgtcagg cgagtctgca aacggtaaat acccactcga gtcagtaact
     5401 acaatggcta caatcgacaa gaacgctcaa gctcttctta atgaatacgg acgtcttgat
     5461 tcagattcat ttgagcgtaa ctctaagaca gaagtaatgg cttctgctgt taaagatgct
     5521 actagctcaa tggatatcaa attggttgta actcttacta agacaggtca tactgcacgt
     5581 ttgatttcta aataccgtcc aaatgctgac atcttagcat tgacatttga cgaattgaca
     5641 gaacgtggct tgatgttgaa ctggggtgtt atcccaatgt tgacagatgc tccatcttca
     5701 actgacgata tgttcgaaat cgctgaacgt aaagcggtag aagcaggtct cgttgagtca
     5761 ggcgatgata tcgttatcgt tgctggtgtg ccagtaggag aagctgttcg cacaaacaca
     5821 atgcgtatcc gcacagtacg ttaagaaaaa tataaaaacc tatcatatcc agctttctag
     5881 cctgtatgat gggctctttt tttgtccagg gggcaaaaag ggggcaaaac tttcataatg
     5941 gattgatact cttcgaaaat caaattcaaa ccacgtcagc gtcgccttac cgtatatatg
     6001 ttactgactt cgtcagttct atctacaacc tcaaagcagt gctttgagca acctgcggct
     6061 agcttcctag tttgctcttt gattttcatt gagtatgaaa taagatatgc ataaattgat
     6121 tagaaagtca aatgaatttc tacaaatgtt ttagcaatcg caatctactt gtctagattc
     6181 gatctgatat attttcgatt taatgatatg gtatttaaaa cctccaaagt agcttgctcc
     6241 attcttttac ctacgtgagt ctagatgtta tttactgttt tagcgttttt gtgttctact
     6301 ctaaccatta tagcattctt ctcagctagt gtactaagga gtgtgtgcct gaaaatatgg
     6361 gaactaaggg gctggtttat cggtttctct agtttagtat ttgccttttg caaagtgatc
     6421 ttaaatgcct ttctctaaat ttacatatca ctattgttta acaaaatcta atctatttta
     6481 ggtcacttat tctttttttg aaatgtagaa tgaacttttt caaagttttt cgaatctttt
     6541 aaaatctgtt tgttttatat cgccattctc cccctttttt aattctccct atatagcctg
     6601 acaacttttc cgatggtacg aatatggttg ctttcgtcta ggtggatgtc ggagtattcg
     6661 ggattgagtt tttttgaggt agccttggcg gagtttcttg acatagttag tgccgtctac
     6721 ttggaagatg ccgatggtat tatagtcaat ctgtggggta ttcttgataa ataggtagtc
     6781 gctgtttctt atctttggct ccatggactt gctgacgaca taagcgattg ggtcgtagtc
     6841 gtctgggata atggaaactc cataactaaa tcgtcgtcct gcatcaagcg gctacctgca
     6901 gagataaact acctaacacg agagtaagta gtctgtctgt agtcgtccag tctgatgatt
     6961 tttacgatac ttcgtttttc tgatcataca gttgcctctc ggcataggtc agaactttac
     7021 cttgtctggg tggtgcccgt tggtcgtaga tagattggat atcgctagga gaatcctttt
     7081 gaactggagg aaagagggca tcaatcaagc tactgaatac tttaactaag tcaaatatag
     7141 tatttttctt agtagaccta accctttttt cataatttct aatggtgttt ttacttatac
     7201 ctatcttagt acccaattct tattgagtcc aaccattact agtctatatt gttttatagt
     7261 tgattgagtt tggaatagta cgctgtagct gctaaaacat ttctaggaat taatttgcct
     7321 ttcctaatag agttgttcat atcttatttc aatctactat atttttcacc tctaacaatc
     7381 gcaatctctt ctttatccat gaacgaaaac gctttctatc tttgtaagta aagcatcaca
     7441 cgaaatccac gaaaatgaaa acctttgttg tgttttcgta aaaaatttgt tgacagagca
     7501 cgaaacgcct actataatta aatcaagttt aaggaaataa caaaaaaaca aatcggaggg
     7561 aaagacgatg aacacattaa atgagaaagt aatcaatatc tgtaaagcag tagttaaaga
     7621 aactttaatc caagacattt aggagaaaga aatgaagaaa tcaagaaaat tagccacatt
     7681 aggaatctgt tcagccttgt ttttaggctt ggctgcttgt caacaacaac atgctacttc
     7741 tgaggggacg aatcaaaggc aaagcagttc agcgaaagtt ccatggaaag cttcatacac
     7801 caacctaaac aaccaggtaa gtacagaaga ggtcaaatct ctcttatcag ctcacttgga
     7861 tccaaatagt gttgatgcat tttttaatct cgttaatgac tataatacca ttgtcggctc
     7921 aactggctta tcaggagatt tcacttcctt tactcacacc gaatacgatg ttgagaaaat
     7981 cagtcatctc tggaatcaaa agaagggcga tttcgttggg accaactgcc gtatcaatag
     8041 ttattgtctt ttgaaaaatt cagtcaccat tccaaagctt gaaaagaatg accagttgct
     8101 tttcctagat aatgatgcga ttgataaagg aaaggtcttt gattcacaag ataaggaaga
     8161 gtttgatatt ctattttcga gagttccaac tgaggcaact acagatgtca aggttcacgc
     8221 tgaaaagatg gaaacattct tctcacaatt tcaattcaat gaaaaagctc gaatgctgtc
     8281 tgtagtcttg cacgacaatc tggatggcga gtatctgttt gtaggccacg ttggggtctt
     8341 agtacctgct gatgacggtt tcttatttgt agagaaattg actttcgaag agccctacca
     8401 agcgattaaa tttgctagta aggaagattg ctacaagtat ttaggcacca agtatgcgga
     8461 ttatacaggc gagggactgg ctaagccttt tatcatggat aatgataagt gggttaaact
     8521 ttaatagtgt tatgatatga cagtaatctt aggattttta tttctatttt tgatgagttg
     8581 gtgggttatt tcaatcgtta atagccagaa accacgcaaa aatgaaacat ttattggcta
     8641 tatccaaaga tacgatattg atgggaatgg ctatcaatca ttaccgtgtc ctcagaactg
     8701 agaggagttc ttgaaatcgt aaaactactt tgaacaagag ttacttcaac ccattggctc
     8761 cgacggatta agttgctttc gtgaacacca aaatcagccg caatttcttc ataagtgcgg
     8821 tattctcgca catattgaag agtggccata agaaggtctt ctaggcttaa tttaggtttt
     8881 cgtccccctt ttgtgtgttt aagttgataa gcagttttta atacagctaa catctcttca
     8941 aaagtggtgc gctgaacacc aacaagacat ttaaatcgtg catcagttag ttgcttactt
     9001 gcttcataat tcatagaact actataccat attttgtttc gcaggaagtc taatgctaaa
     9061 atcttatcag tgagaagatt tcttaaagtt tattctttgt agtttcatct gtattagtac
     9121 ataatttaac ttagagaaaa taggaggact aatcgaatgg aattaaaaga ttttacagaa
     9181 aaagaacagg aaatgattaa gaaaaggctt acaatgtcta atattagtga caaagaaact
     9241 actgagaaga ttctggcgct agtaccacaa gacttgatta agcgaatccc gttttttgtc
     9301 agaaaacatg ctacaacacg tacgattaaa cgcatttcaa ttgaacaccc tgaactctac
     9361 gctgtagctc aaacaagtgg tgagattcca gaaaaagaac gcgaagaatt gcgtcagatt
     9421 atcacaacta tctttgaaca aaagatgaat aagcatagta ttaagtagag aatgaaaaaa
     9481 tattttattg gcggtttggg aaataatgcc tatcatagca aggattttct tcaagaacta
     9541 gattcgcagg tctattttct aaatccatat gaaaagcatc ttcgagatga aacagaattg
     9601 aaatcatggt ttaaaaatga gattgtagag gaagaatcta tctgtctgat aggccattct
     9661 cttggaggag atttagctcg ttatctcaca tcggaatttg aagaagtaaa aaaactgatt
     9721 cttttggatg gtggctatct agatttagat aagattttac ctttggatac agagttagag
     9781 gaaactaaaa attatatcaa atctcaaatt gttttggact tagctcttct tatttctaaa
     9841 gaaaaatctg gagcaaagca ttggtcagaa aatatggaga aagctgtaag acagtcttat
     9901 cactggaatg ttgagtataa tagatatgag ttggctataa attatgaaaa tatagaagcg
     9961 atactccgcc tacggagaaa aatacaagct tttaagagag aagtgggaga taccttgttt
    10021 attagtcctc gctatcctaa tgaagctaca tggagagagg aagccctaaa agaattgcca
    10081 gactattttg atactatttt tttagaaaac tttggccatg agctttatac tcaagcacct
    10141 aaagaaatcg ctagtctaat gaatgagtgg ctcgcttatt ttctatgagc tacttgtatt
    10201 gctggaaatg gtataagatg aaaatctaca agtcactaat tttcatataa ccaaaaaacg
    10261 aacaggctct ttttctgtta ttcagaagag aacttgttcg tttttatctt gattttctta
    10321 aaaagctcta tatacatagg gattttctgg tttatcgact ttggtaaagc tgtcgatttc
    10381 caaggttgga aggttttgtt tgagttcttt atggtagtga ttgaccagtt ttcctttggc
    10441 tgaaatccaa gtgttgtttt catactgccg ggtattgccc ttggtcagca atccatagac
    10501 accagaatct gcgatacagt ggataatgcc gaatcggaac agaaattgac tattggcatg
    10561 actggggtcg ttatagacaa agcctgtaaa ctggattgtc ttgccctcaa actcatctgg
    10621 atagtcgtag atagcctcca tgacttccat atagttttca ttagtgatct gaatactatc
    10681 ttgggataag tatttatccg ccgccgttcg catttccttt tcataggctg attttgaaaa
    10741 ataagaactg gtatctggtt tcaaatattg gcttgtcgtc ccttcgcttg tctgaatggc
    10801 tagatccgtt ccttccgata gggggaaatg ataaccttta gcagaaacag tctgagaatc
    10861 caagctaaca gttgggaaag ttaagccaat gacaagtgga atagctagaa gagaaatact
    10921 cgtcatcttg gccaatcggc tgttcagatg actgtgggtt ttgacttgct tcatccagat
    10981 atacaattga acaatagcca agataaagga aagcaccatg gaaatatagg ccagatagga
    11041 atagtgcatg ttgatgtact ggtttagttt gcccgacaga tggaggtaaa tagtcagttc
    11101 aaaatagcca gctaaaacta aaaatcgaat cataggatga ctccaatcaa gagagaatag
    11161 gctaaaacga ccagagtcac aatcgtaatg aagtgactaa taaatcgtgc tttcaagtaa
    11221 tttttcatca tgagaatatt tttgatatcc agcattgggc caatgacgag aaaggccaga
    11281 actggagcca aaccgaaact cgagagtaga gaagcaccta taaaggcatc cgcctcacta
    11341 cagagcgaaa gaagaaaggc caaaagcatc aagagcagga tggcaaaaag aggagtcgca
    11401 ctgatagagg tcagaatccg agtcggaacg tagacctgta ttatagaagc aaagaggcag
    11461 ccaaatacca aataacgccc cgtatcaaaa aattcatcaa tggcctgcac aaagacttga
    11521 aaaacttttt ttgcagaact caagtaagaa aaatcatgct catgacaagc cagacgattt
    11581 tctttctgaa tcggttcttg ccagaaaaat cctagaaaaa ttcctagtat tacagccaca
    11641 agaatggaac ccagagctcg taatagggcg acatggaagg agttgccaaa ggcagaatag
    11701 gtcgcaaaaa gaacaatggg attgataaca ggtgctgtca caagaaaagg aacggccgtg
    11761 taacttggaa cctttttttc cagaaaacga ttgatgatgg ggacgattcc acattcacaa
    11821 gaagggaaaa gtataccgac aaaggtccca aaaaagattc tcccccaacg atttcgaggg
    11881 agaaaatgat aaaccttgtc aggtgtgata taaacttcaa tcagccctga gacaatgctt
    11941 cctatcagaa cgaagggaag ggcttcaatg ataatggaga gaaaaatggc tccagtttga
    12001 agtacactag atgggagttg ttgaaaagca gtcatctagt ttttcttttc tgctttgact
    12061 tttttctctt tgtcatcagg gaaagtaact tgtttcaaat cagggagttt agcaaactct
    12121 tcaaacatct tgtccaagtc gtctcgtttt gtaaatttga cagccatagg tgtacctcga
    12181 ttctaaattc tacctctatt atactattat tttgcggaaa agggagtttt ttaatttgtt
    12241 ttcgatagtt gatacacttc gaaaatctct tcaaactacg tcagcttcgc cttaccgtag
    12301 ta
//
LOCUS       DAVKLW010000058        12243 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKLW010000058 DAVKLW010000000
VERSION     DAVKLW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.058"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            206..2866
                     /gene="rrgA"
                     /locus_tag="VW324_001689"
     CDS             206..2866
                     /gene="rrgA"
                     /locus_tag="VW324_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU9727270.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3090..5054
                     /locus_tag="VW324_001690"
     CDS             3090..5054
                     /locus_tag="VW324_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEU9727271.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAEQKTKTLTVHKLLM
                     TDQELDAWNSDAITTAGYDGSQNFEQFKQLQGVPQGVTEISGVAFELQSYTGPQGKEQ
                     ENLTNDAVWTAVNKGVTTETGVKFDTEVLQGTYRLVEVRKESTYVGPNGKVLTGMKAV
                     PALITLPLVNQNGVVENAHVYPKNSEDKPTATKTFDTAAGFVDPGEKGLAIGTKVPYI
                     VTTTIPKNSTLATAFWSDEMTEGLDYNGDVVVNYNGQPLDNSHYTLEAGHNGFILKLN
                     EKGLEAINGKDAEATITLKYTATLNALAVADVPEANDVTFHYGNNPGHGNTPKPNKPK
                     NGELTITKTWADAKDAPIAGVEVTFDLVNAQTGEVVKVPGHETGIVLNQTNNWTFTAT
                     GLDNNTEYKFVERTIKGYSADYQTITETGKIAVKNWKDENPEPINPEEPRVKTYGKKF
                     VKVDQKDERLKEAQFVVKNEQGKYLALKSAAQQAVNEKAAAEAKQALDAAIAAYTNAA
                     DKNAAQAIVDAAQKTYNDNYRAARFGYVEVERKEDALVLTSNTDGQFQISGLAAGSYT
                     LEETKAPEGFAKLGDVKFEVGAGSWNQGDFNYLKDVQKNDATKVVNKKITIPQTGGIG
                     TIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            5101..6282
                     /gene="rrgC"
                     /locus_tag="VW324_001691"
     CDS             5101..6282
                     /gene="rrgC"
                     /locus_tag="VW324_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU9727272.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKTN
                     N"
     gene            6621..7523
                     /gene="srtC-1"
                     /locus_tag="VW324_001692"
     CDS             6621..7523
                     /gene="srtC-1"
                     /locus_tag="VW324_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU9727273.1"
                     /translation="MAKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            7510..8403
                     /gene="srtC-2"
                     /locus_tag="VW324_001693"
     CDS             7510..8403
                     /gene="srtC-2"
                     /locus_tag="VW324_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU9727274.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEEILQKGAGLLEGASLPVGGENTHTVVTAHR
                     GLPTAELFSQLDKMKKGDVFYLHVLDQVLAYQVDQILTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLAALVMILVLSYGV
                     YRHRRIVKGLEKQLEEHHVKG"
     gene            8390..9241
                     /locus_tag="VW324_001694"
     CDS             8390..9241
                     /locus_tag="VW324_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEU9727275.1"
                     /translation="MSKAKLQKLLGYLLMLVALVIPVYCFGQMVLQSLGQVKGHEIFS
                     ESVTADSYQEQLQRSLDYNQRLDSQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLAMGLAHVDGTPLPVEGKGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(9314..10590)
                     /locus_tag="VW324_001695"
                     /pseudo
     CDS             complement(9314..10590)
                     /locus_tag="VW324_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10705..10845)
                     /locus_tag="VW324_001696"
     CDS             complement(10705..10845)
                     /locus_tag="VW324_001696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727276.1"
                     /translation="MKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVDAFM
                     AL"
     gene            complement(10847..11131)
                     /locus_tag="VW324_001697"
     CDS             complement(10847..11131)
                     /locus_tag="VW324_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727277.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
BASE COUNT         3871 a         1986 c         2763 g         3623 t
ORIGIN      
        1 aagtgtgtca ttgccaggtt gagaagatag ctataacgca cttttatacg cttttgctac
       61 gtttgttagt gaacggatta actcagtgag ataaatttta tcagaacata agtaatccgt
      121 ttcttcgtgt atacagattg aaagtaccta tgaatcatag aaggattaac ttgttctatg
      181 aataatgctt aacagggaga cacacatgaa aaaagtaaga aagatatttc agaaggcagt
      241 tgcaggactg tgctgtatat ctcagttgac agctttttct tcgatagttg ctttagcaga
      301 aacgcctgaa accagtccag cgataggaaa agtagtgatt aaggagacag gcgaaggagg
      361 agcgcttcta ggagatgccg tctttgagtt gaaaaacaat acggatggca caactgtttc
      421 gcaaaggaca gaggcgcaaa caggagaagc gatattttca aacataaaac ctgggacata
      481 caccttgaca gaagcccaac ctccagttgg ttataaaccc tctactaaac aatggactgt
      541 tgaagttgag aagaatggtc ggacgactgt ccaaggtgaa caggtagaaa atcgagaaga
      601 ggctctatct gaccagtatc cacaaacagg gacttatcca gatgttcaaa caccttatca
      661 gattattaag gtagatggtt cggaaaaaaa cggacagcac aaggcgttga atccgaatcc
      721 atatgaacgt gtgattccag aaggtacact ttcaaagaga atttatcaag tgaataattt
      781 ggatgataac caatatggaa tcgaattgac ggttagtggg aaaacagtgt atgaacaaaa
      841 agataagtct gtgccgctgg atgtcgttat cttgctcgat aactcaaata gtatgagtaa
      901 cattcgaaac aagaatgctc gacgtgcgga aagagctggt gaggcgacac gttctcttat
      961 tgataaaatt acatctgatc cagaaaatag ggtagcgctt gtgacttatg cttccactat
     1021 ctttgatggg accgagttta cagtagaaaa aggggtagca gataaaaacg gaaaacgatt
     1081 gaatgattct cttttttgga attatgatca gacgagtttt acaaccaata ccaaagatta
     1141 tagttattta aagctgacta atgataagaa tgacattgta gaattaaaaa ataaggtacc
     1201 taccgaggca gaagaccatg atggaaatag attgatgtac caattcggtg ccacttttac
     1261 tcagaaagct ttgatgaagg cagatgagat tttgacacaa caagcgagac aaaatagtca
     1321 aaaagtcatt ttccatatta cggatggtgt cccaactatg tcgtatccga ttaattttaa
     1381 tcatgctacg tttgctccat catatcaaaa tcaactaaat gcatttttta gtaaatctcc
     1441 taataaagat ggaatactat taagtgattt tattacgcaa gcaactagtg gagaacatac
     1501 aattgtacgc ggagatgggc aaagttacca gatgtttaca gataagacag tttatgaaaa
     1561 aggtgctcct gcagctttcc cagttaaacc tgaaaaatat tctgaaatga aggcggctgg
     1621 ttatgcagtt ataggcgatc caattaatgg tggatatatt tggcttaatt ggagagagag
     1681 tattctggct tatccgttta attctaatac tgctaaaatt accaatcatg gtgaccctac
     1741 aagatggtac tataacggga atattgctcc tgatgggtat gatgtcttta cggtaggtat
     1801 tggtattaac ggagatcctg gtacggatga agcaacggct actagtttta tgcaaagtat
     1861 ttctagtaaa cctgaaaact ataccaatgt tactgacacg acaaaaatat tggaacagtt
     1921 gaatcgttat ttccacacca tcgtaactga aaagaaatca attgagaatg gtacgattac
     1981 agatccgatg ggtgagttaa ttgatttgca attgggcaca gatggaagat ttgatccagc
     2041 agattacact ttaactgcaa acgatggtag tcgcttggag aatggacaag ctgtaggtgg
     2101 tccacaaaat gatggtggtt tgttaaaaaa tgcaaaagtg ctctatgata cgactgagaa
     2161 aaggattcgt gtaacaggtc tgtaccttgg aacggatgaa aaagttacgt tgacctacaa
     2221 tgttcgtttg aatgatgagt ttgtaagcaa taaattttat gataccaatg gtcgaacaac
     2281 cttacatcct aaggaagtag aacagaacac agtgcgcgac ttcccgattc ctaagattcg
     2341 tgatgtgcgg aagtatccag aaatcacaat ttcaaaagag aaaaaacttg gtgacattga
     2401 gtttattaag gtcaataaaa atgataaaaa accactgaga gatgcggtct ttagtcttca
     2461 aaaacaacat ccggattatc cagatattta tggagctatt gatcaaaatg gcacttatca
     2521 aaatgtgaga acaggtgaag atggtaagtt gacctttaaa aatctgtcag atgggaaata
     2581 tcgattattt gaaaattctg aaccagctgg ttataaaccc gttcaaaata agcctatcgt
     2641 tgccttccaa atagtaaatg gagaagtcag agatgtgact tcaatcgttc cacaagatat
     2701 accagcgggt tacgagttta cgaatgataa gcactatatt accaatgaac ctattcctcc
     2761 aaagagagaa tatcctcgaa ctggtggtat cggaatgttg ccattctatc tgataggttg
     2821 catgatgatg ggaggagttc tattatacac acggaaacat ccgtaaagtg tagaaatgat
     2881 aatatctatg ttctgaacga tacttttaag aagtagcact caagaagaga tttaagttta
     2941 cttggtgaaa cctgttttat tcgtaagtaa actatcattg aaaggggaga tgttttcgaa
     3001 aacttgcaca gaaaaaggat tattattgtc atgtgtaatt cattacattg ctcacagttg
     3061 attttaagag atatgaataa ggagaaatca tgaaatcaat caacaaattt ttaacaatgc
     3121 ttgctgcctt attactgaca gcgagtagcc tgttttcagc tgcaacagtt tttgcggcgg
     3181 aacaaaaaac taagacactt acagttcata aattattgat gacagatcaa gagcttgacg
     3241 cttggaattc tgatgcgatt actactgcag gttatgacgg ttcgcaaaat tttgaacagt
     3301 tcaaacaact tcaaggtgtt ccacaaggag taaccgaaat ctctggtgtt gcattcgagt
     3361 tacagagtta tacgggtcct caaggaaaag aacaagaaaa tttaacgaat gatgcggttt
     3421 ggactgcggt taataaaggt gtgacgactg aaacgggtgt taaatttgat actgaagttt
     3481 tacaagggac atatcgtctt gtcgaagtac gtaaagaatc gacttatgtc ggtccaaatg
     3541 gtaaagtttt aacaggtatg aaagctgttc ctgctttaat tactctgccg cttgtaaacc
     3601 aaaatggtgt tgtagaaaat gcacatgtct atccaaagaa ttctgaagac aaacctacag
     3661 caacgaaaac atttgatact gcagcaggtt tcgtagatcc aggtgaaaaa ggtttagcaa
     3721 ttggcactaa ggtaccgtat attgttacaa caactattcc gaaaaactca actcttgcaa
     3781 cagctttctg gtcagatgaa atgacagaag gtctagatta taatggtgat gtagttgtta
     3841 attataatgg tcaaccgctt gataattctc attacacatt agaagcaggt cataatggct
     3901 ttatcttgaa gttaaatgaa aaaggtctgg aagcaatcaa cggtaaagat gcagaagcaa
     3961 caattacgtt gaagtatact gcaactttaa atgctcttgc tgttgctgat gtgccagaag
     4021 cgaatgatgt aacattccat tatggaaaca acccaggtca tggtaacact ccaaaaccaa
     4081 acaaacctaa aaacggtgaa cttacaatta ctaaaacatg ggctgatgct aaagatgctc
     4141 ctatagcagg tgtagaagta acttttgatt tggtaaatgc tcagacaggt gaggtcgtta
     4201 aagtacctgg acatgaaaca ggtattgtat tgaatcaaac aaataattgg acatttactg
     4261 ctacaggtct tgataataat acagaatata aatttgttga acggacaatt aagggatatt
     4321 ctgcagatta ccaaacaatt actgaaacag gaaaaattgc tgttaaaaac tggaaagatg
     4381 aaaatccaga accaataaat cctgaagagc cacgtgtaaa aacatatggt aaaaaattcg
     4441 ttaaggttga ccaaaaagac gaacgcttaa aagaagcaca attcgttgtg aagaatgagc
     4501 aagggaaata tcttgcactc aaatctgcag cacaacaagc tgtaaatgag aaagctgccg
     4561 cagaagcgaa acaagcgcta gatgcagcga tagcagccta tacaaatgct gcagataaaa
     4621 atgcagcaca agctatagta gatgctgcgc aaaaaacata taatgacaat tacagagcag
     4681 ctagatttgg ctatgtagaa gtagagagaa aagaagatgc gttagttctt acttctaaca
     4741 ctgatggtca attccaaatt tcaggtcttg ctgctggaag ctacacgttg gaagaaacaa
     4801 aagctccaga aggctttgca aaacttggag atgtgaagtt tgaggttgga gcaggttctt
     4861 ggaatcaagg tgatttcaat tatttaaaag atgttcagaa gaacgacgct acaaaagtag
     4921 tcaacaaaaa aatcacgatc cctcaaacgg gtggtattgg tacaattatc tttgctgtag
     4981 cgggggctgt gattatgggt attgcagtgt acgcatatgt taaaaacaac aaagatgagg
     5041 atcaacttgc ttaagtaaga gagaaaggag ccattgatga caatgcagaa aatgcagaaa
     5101 atgattagtc gtatcttctt tgttatggct ctgtgttttt ctcttgtatg gggtgcacat
     5161 gcagtccaag cgcaagaaga tcacacgttg gtcttgcaat tggagaacta tcaggaggtg
     5221 gttagtcaat tgccatctcg tgatggtcat cggttgcagg tatggaagtt ggatgattcg
     5281 tattcctatg ataatcgggt gcaaattgtg agagacttgc attcgtggga tgagaataaa
     5341 ctttcttctt tcaaaaagac ttcgtttgag atgaccttcc ttgagaatca gattgaagta
     5401 tctcatattc caaatggtct ttactatgtt cgctctatta tccagacgga tgcggtttct
     5461 tatccagctg aatttctttt tgaaatgaca gatcagacgg tagagccttt ggtcattgta
     5521 gcgaaaaaag cagatacggt gacaacaaag gtgaagctga taaaggtgga tcaagaccac
     5581 aatcgcttgg agggtgtcgg ctttaaattg gtatcagtag caagagatgg ttctgaaaaa
     5641 gaggttccct tgattggaga ataccgttac agttcttctg gtcaagtagg tagaactctc
     5701 tatactgata aaaatggaga gattgttgtg acaaatcttc ctcttgggac ctatcgtttc
     5761 aaggaggtgg agccactggc aggctatact gttacgacga tggatacgga tgtccagttg
     5821 gtagatcatc agctggtgac gattacggtt gtcaatcaga aattaccacg tggcaatgtt
     5881 gactttatga aggtggatgg taggaccaat acctctcttc aaggggcaat gttcaaagtc
     5941 atgaaagaag aaaacggaca ctatactcct gttcttcaaa atggtaagga agtagttgtg
     6001 gcatcaggga aagatggtcg tttccgagtg gaaggtctag agtatgggac atattattta
     6061 tgggagctcc aagctccaac tggttatgtt caattaacat cgcctgtttc ctttacaatc
     6121 gggaaagata ctcgtaagga actggtaaca gtggttaaaa ataacaagcg accacggatt
     6181 gatgtgccag atacagggga agaaaccttg tatatcttga tgcttgttgc cattttgttg
     6241 tttggtagtg gctattatct tacgaaaaaa acaaataact gatattcaat gtacatcatt
     6301 atgaaaaaga tagcaggctg aagggaagac cagagtactc tgaggtgatg ttaatcagga
     6361 atcatggtga tttggcatga atcacaataa cggatatgag gctgggcaga ttgtgccagc
     6421 ctcattgtgg gttattgttt gtaaaacgat aggactggtc tggtaatcat tttaggaatg
     6481 gacaggactg ggattctgat ttaaaatgga tggtgaatca gaaagaaatg agattttctc
     6541 gtttctctta gcagatagga ttgtctgtta ggaaaagcga taaaatgatg agtttgaagt
     6601 taaaggaatg ctgataaaaa atggcaaaaa caaaaaagca aaaacgaaac aatctcctat
     6661 taggagtggt atttttcatt ggaatggcgg taatggcgta tccgctggtg tctcgcttgt
     6721 attatcgagt ggaatcaaat caacaaattg ctgactttga taaggaaaaa gcaacgttgg
     6781 atgaggctga cattgatgaa cgaatgaaat tggcacaagc cttcaatgac tctttgaata
     6841 atgtagtgag tggcgatcct tggtcggaag aaatgaagaa aaaagggcga gcagagtatg
     6901 cacgtatgtt agaaatccat gagcggatgg ggcatgtgga aatccccgtt attgacgtgg
     6961 atttgccggt ttatgctggt actgctgaag aggtattgca gcaaggggct gggcatctag
     7021 agggaacttc tctgccgatc ggaggcaatt cgacccatgc ggtgattacg gcacatacag
     7081 gtttgccaac agctaagatg tttacggatt tgaccaaact taaagttggg gataagtttt
     7141 atgtgcacaa tatcaaggaa gtgatggcct atcaagtgga tcaagtaaag gtgattgagc
     7201 cgacgaactt tgatgattta ttgattgtac caggtcatga ttatgtgacc ttgctgactt
     7261 gtacgccata catgatcaat acccatcgtc tattggttcg ggggcatcgg ataccgtacg
     7321 tagcagaggt tgaggaagaa tttattgcag caaacaaact cagtcatctc tatcgctacc
     7381 tgttttatgt ggcagttggt ttgattgtga ttcttttatg gattattcga cgcttgcgca
     7441 agaagaaaaa acaaccggaa aaggctttga aggcgctgaa agcagcaagg aaggaagtga
     7501 aggtggagga tggacaacag tagacgttca cgaaaaaaag gcacaaaaaa gaagaaacat
     7561 ccgctgatcc ttcttctgat tttcttagta ggattcgccg ttgcgatata tccattggtg
     7621 tctcgttatt attatcgtat tgagtcaaac gaggttatta aagagtttga tgagacggtt
     7681 tcccagatgg ataaggcaga acttgaggag cgttggcgct tggctcaagc cttcaatgcg
     7741 accttgaaac catctgaaat tcttgatcct tttacagagc aagagaaaaa gaaaggcgtc
     7801 tcagaatatg ccaatatgct aaaggtccat gagcggattg gctatgtgga aattcctgcg
     7861 attgatcagg aaattccgat gtatgtcgga acgagtgagg aaattcttca gaagggcgca
     7921 ggattgctag agggagcttc gttaccggtt ggtggtgaaa atacccacac agttgtcact
     7981 gctcatagag gattaccgac ggcagaactg tttagtcaat tggataagat gaaaaaaggg
     8041 gatgtctttt atcttcacgt tttagaccag gtgttggcct accaagtgga tcagattttg
     8101 acggttgagc caaatgactt tgagcctgtc ttgattcaac atggggaaga ttatgcgacc
     8161 ttgttgacct gtacaccgta tatgattaac agtcatcgtc tgttggtacg tgggaagcgg
     8221 attccgtata cggcaccaat tgcagagcga aatcgagcgg tgagagagcg tgggcaattc
     8281 tggttgtggt tattgctagc ggcgttggtt atgattctgg tattgagtta cggggtgtat
     8341 cgtcatcgtc gcattgtcaa agggctagaa aaacaattgg aggagcatca tgtcaaaggc
     8401 taagctacag aaattactag ggtatttgct gatgctggta gcattggtga ttcctgttta
     8461 ttgttttggg cagatggtgt tacagtcttt aggacaagta aaaggtcatg agatattttc
     8521 agaatctgtg acggccgaca gttaccaaga gcaattgcaa cggtcgcttg attacaatca
     8581 acgcttggat tcgcaaaatc gtattgtaga tccttttttg gcggaagggt atgaggtaaa
     8641 ttaccaagtg tctgacgatc ctgatgcagt ctacggctat ttgtcgattc cgagtttgga
     8701 aatcatggag ccagtttatc taggagcgga ttaccatcat ttagcaatgg ggttggccca
     8761 tgtggatggg acgcctcttc ctgttgaggg aaaagggatt cgttcagtga ttgctgggca
     8821 ccgtgcagaa ccaagccatg tctttttccg ccatttggat cagctaaaag ttggagatgc
     8881 tctttattat gataatggcc aggaaattgt agaatatcag atgatggaca cagagattat
     8941 tttaccgtcg gaatgggaaa aattagaatc ggttagctct aaaaatatca tgaccttgat
     9001 aacctgcgat ccgattccta cctttaataa acgcttatta gtgaattttg aacgagtcgc
     9061 tgtttatcaa aaatcagatc cacaaacagc tgcagttgcg agggttgctt ttacgaaaga
     9121 aggacaatct gtatcgcgtg ttgcaacctc tcaatggttg taccgtgggc tagtggtact
     9181 ggcatttctg ggaatcctgt ttgttttgtg gaagctagca cgtttactac gagggaaata
     9241 aaaagaaatg aaaggaaagc taaggctgtt cctttttccg gctctttgtc aactgtagtg
     9301 ggttgaaaaa aagctaagct cgagaaagga caaattttgt cctttctttt ttgatattca
     9361 gagcgataaa aatccgtttt ttgaagtttt caaagtttcg aaaaccaaag gcattgcgct
     9421 tgataagttt gatgagatta ttggtcgctt ccagtttggc attagaatag tgtagttgaa
     9481 gggcgttgat aatcttttct ttatctttga ggaaggtttt aaagacagtc tgaaaaatag
     9541 gatgaacctg cttaagattg tcctcaataa gtccgaaaaa tttctctggt tccttattct
     9601 ggaagtgaaa cagcaagagc tgatagagct gatagtggtg tttcaagtct tgtgaatggc
     9661 tcaaaagctt gtctaaaatc tctttattgg ttaagtgcat acgaaaagta ggacgataaa
     9721 atcgcttatc actcagtcta cggctatcct gttgaatgag tttccagtag cgcttgatag
     9781 ccttgtattc atgggatttt cgatgaaact gattcatgat ttggacatgc acacgactca
     9841 tagcacggct aagatgttgt acaatgtgaa agcgatcaag aacgatttta gcacacggga
     9901 aaagttgtct agctaagtca tagtaaggac taaacatatc catagtaatg attttgacgc
     9961 gacatcggac ggctctatca tatttaagaa agtgatctcg gatgacagct tgtgttctac
    10021 cttcaagaac agtgatgata tcgagctttt caaaatcttg cgcaataaag ctcatctcca
    10081 tctcccgatt gaaacagtca ctccccggac tgtttcaacg tcccaggaca taatctcagg
    10141 aagacgcgaa aaatcatgct caaagtgaga atcattgagc ttgcgaatga cagttgaagt
    10201 tgaaatggcc agctgatgag caatatcggt catagaaatc ttctcaatca acttttgcgc
    10261 aattttttgg ttgataatac gaggaatttg atgattcttc ttgacgatag aagtttcagc
    10321 gaccatcatt tttgaacagt gatagcactt gaaacggcgt tttctaagga gaattctaga
    10381 aggcatacca gttgtttcga ggtaagggat cttagacggt ttttgaaagt catatttctt
    10441 caattggttt ccgcactcag ggcaagatgg ggcgtcgtag tccagtttgg cgatgatttc
    10501 cttgtgtgta tccttattga tgatgtctaa aatctggata ttagggtctt taatgtctag
    10561 taattttgtg ataaaatgta attgttccat atgattcttt ctaatgagtt gttttgtcgc
    10621 ttttcattat aggtcatatg ggactttttt tctacacaaa aataggctcc ataatatcca
    10681 taggggattt acccactaca aatattatag agccataaaa gcatcaacat cttgtgtggc
    10741 acgtaaacca tgatattcta agacataacc accgacacag ataattgtta aagtcagatt
    10801 ttcctgaatt aattccttat ttagggcttg gaagaccggt ttcattttat ccatgtttgt
    10861 atttctccat caatttttga acattggtta acttagcagg tttatttaat gaaatcacat
    10921 atttatgagt tggagtatgc tcttgaaagg catacttcaa gctaattttt tgaaaacggg
    10981 ttagttcttt ggagtctagc aacttttcta catcttggtt tgttaaataa gaatagtctt
    11041 caaaaataac ttttcgcaaa tagcgaaacc aatggcttgc gttgaattgt gattgtttgg
    11101 atgcctgtat catataatcg taccaatcca ttgtcttcct cctataaaat ttagtttata
    11161 ttattgtagt ttatattcaa aaatgtagtt tttcttaatt agctcgtatc tataggatga
    11221 tactgaaaat cttcttgttg ctttaaatca caatcgaaaa attctgtttt tcatcttaga
    11281 aaagaatgtt tagaaatgta aaatgaacct tttttcttat ccgaaaacct tactaaagta
    11341 tataatttag gaagtcaatc ctgtatgata atgtttgtac tatcgtcgag tttttgcttg
    11401 attgcctaac gagataaatt ttagaagaat tttcggtgtg atttgacaag cttctgactg
    11461 gaaggatagt tacactagta ttgtttatta aaatgagcag atagagaaaa tttggaatct
    11521 atttgcttat tatcaatcgt ttttttagct tatttgtctt agattggtat agaactgagc
    11581 tatggattaa tactcttgta tcttatcctg ttcctaaata tgttggtaga aaaatgaagc
    11641 agttgttacg agtgtctgta ttggaaaaaa ttttgtctat agaattcttt aaatgatatg
    11701 aaggctagga atgccaaagc actgacatat ttcgtttgaa tctcatccgc tctctttact
    11761 tgtatttgta gaaatgctat cagtaatata ttcgcgaaca ctatgattta caacagtgtg
    11821 gtcgtacatc tttgtaactt tattatacaa caaccattta caggtgtaaa gttgagcagt
    11881 gtctttagta aatttaggga tttgttttgt catagttttg atacctgtat tcgttgtttc
    11941 atgagcattt gacgtttctt ttcatgtagg catttcaaca attagaatga tatttttccg
    12001 aggatgaact tatatattgt gactttttag agttatgatg cctcttgatt tgactatcgc
    12061 tatcaatttc atccactttt acagtttcat ggttttatga accgaaacat ctaggtacag
    12121 gttttagcat caattgattg tttttgagcc atatgaagtt gaaacgtgga gatgcttttc
    12181 ttgtgataac gccctattca aagaggaata accgtttttg gagagagttc agtactatgt
    12241 ggc
//
LOCUS       DAVKLW010000059        11920 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKLW010000059 DAVKLW010000000
VERSION     DAVKLW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.059"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..169
                     /locus_tag="VW324_001698"
     CDS             <1..169
                     /locus_tag="VW324_001698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727278.1"
                     /translation="SIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHDKPTLTWFEE
                     IFEEYHSPFHN"
     gene            complement(503..1639)
                     /gene="dnaJ"
                     /locus_tag="VW324_001699"
     CDS             complement(503..1639)
                     /gene="dnaJ"
                     /locus_tag="VW324_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9727279.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(2041..2397)
                     /locus_tag="VW324_001700"
     CDS             complement(2041..2397)
                     /locus_tag="VW324_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9727280.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(2399..4222)
                     /gene="dnaK"
                     /locus_tag="VW324_001701"
     CDS             complement(2399..4222)
                     /gene="dnaK"
                     /locus_tag="VW324_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9727281.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4702..5226)
                     /gene="grpE"
                     /locus_tag="VW324_001702"
     CDS             complement(4702..5226)
                     /gene="grpE"
                     /locus_tag="VW324_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9727282.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(5253..6287)
                     /gene="hrcA"
                     /locus_tag="VW324_001703"
     CDS             complement(5253..6287)
                     /gene="hrcA"
                     /locus_tag="VW324_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9727283.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(6455..6961)
                     /locus_tag="VW324_001704"
     CDS             complement(6455..6961)
                     /locus_tag="VW324_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9727284.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREDLSMSFFLIFCFICFSDFLSNSWLFQ
                     TKKEIPSR"
     gene            7569..9902
                     /locus_tag="VW324_001705"
     CDS             7569..9902
                     /locus_tag="VW324_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9727285.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            9915..11378
                     /locus_tag="VW324_001706"
     CDS             9915..11378
                     /locus_tag="VW324_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9727286.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            11378..>11920
                     /locus_tag="VW324_001707"
     CDS             11378..>11920
                     /locus_tag="VW324_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9727287.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVL"
BASE COUNT         3672 a         2329 c         2313 g         3606 t
ORIGIN      
        1 ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa aacaccgttt
       61 taaggttgtg gatagaactg acgaagtcag taaccatacc catgacaagc cgacgctgac
      121 gtggtttgaa gaaatttttg aagagtatca ttcaccattt cacaactaaa tcgaagactt
      181 tctgccgtaa tgaaaacacc tgaaacagct tggtttcggc tatccggaaa ctttaagact
      241 taggttcaaa gtttaggtat ggaacttcga aggaggtcgc taccgtccgt cattacttag
      301 gaaagtctta aaaaatctat aaagtaaaaa gagcccgaca gctctttttg tttatactca
      361 atgaaaatca aaaagcaaac taggaaacta gccacgggct gctcaaaaca ctgttttgag
      421 gttgtagata cgactgacga agtcagtcac atatatacgg caaggcaacg ctgacgtggt
      481 ttgaagagat tttcgaagag tattattctc catcaaaggc atctttaata tggtcaaaga
      541 agcctttttt ctttggattt actttcaagt caccagcagc cgcgaattct ttcaaggcta
      601 ctttttggcg gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg tattggtcac
      661 caactgcacc gccacgaagg ctcggtgccc ccttactacg taggcggaac ttcttaccag
      721 tctgagttcc ctctggaata accaattcaa catcaccgtg aacagttgga atatctactg
      781 tatcaccaag agccgcttgg acaaagttga ggttgagatt gtagaagata gtcgttcctt
      841 cacgttcaaa cttgtcgcta gcttccacag aaactactac atacaagtca ccataaggtc
      901 caccgttaaa gcctgcttca ccttgaccag cgaggcgaat ttgttgacct gtttccacac
      961 cagcagggat tttcacatgt acgctatgag cttgtttctc atgacctgtt ccatgacagg
     1021 ttgtacatgg atatttgatt tcttttcctc gaccgtgaca gacatcacag gttacttggc
     1081 gacgcatcat accaagagga gtctgcgtat cgacgttaat gacaccagcg ccatgacagc
     1141 gtccacaagt gactggactt gtccctggct tagcaccaga tccattacat gtacgacagc
     1201 cagcttcacg atgatactta acttccttct cagttccgaa gatagcttct tcaaaggtca
     1261 aattgacacg atactggaga tcatctcctt ggcgaggagc gtttggattg cgcgaagaac
     1321 cgcctccgcc gaagaaactt gagaaaatat cctcaaaacc accgaagcca cctgccccat
     1381 tgaaaccgcc gaaaccacca gctccaccaa aaccaccatt ggcgcctgca gcaccatact
     1441 ggtcataggc agcacgtttt tggtcgtcac tcaaagtctc ataggcttct tgaacttcct
     1501 tgtacttgtc ctcagcacca ggctccttgt tgatatctgg gtgatatttt ttggaaagct
     1561 tacgataagc ctttttgatt tcgtctgccg aagcgttttt ggataccccc agacgatcat
     1621 aaaattcagt attgttcata attcaagata caaagggcgt caaacggaca cagccatcta
     1681 ggagttttga cgacggacgc ttacgtccta gaaaaaacta tctttttggc acagtccata
     1741 gcccgtgttc agttacgaac acccttattc ctttctattg atgttctgaa tcgaaccacc
     1801 atttaggtcg ggttcaattc ctttctttta tatttagtaa gaaattctga aacccgtgtt
     1861 cagttacgaa cactctttgt tcctttctat tgatgagaca aaacccaaac aaatgagatt
     1921 ccgtcccttt ttatcgaaaa acagaggctg ggttgcaacc tctgcaaata agtaaattaa
     1981 ctttattttg attttaaata tcctataagt acagaaatat ttatatattt ttgtctcttc
     2041 ttagtatttg cctatataat tccaattgat cataagaaat acaactactg tccaaatcat
     2101 accaattatg aacggtatta caaatttata tgtttgtatt tctaatgata tcatagctga
     2161 taccatcgct gatacaaatg cagacaacaa tactgaagta acaaaattcc ttaccgcttc
     2221 atcatttttg acacaactat catgctcagc taatataatt tctctatata tagtcaaaca
     2281 ttctactgga atattatcta taaaaccttt tgctgcttca tagttgtcag cttgattaca
     2341 aaaatcatta taaacttttc gtgtattttt tagatactca tcatccaata cactcatctt
     2401 atttttccgt aaactctccg tctacgacgt catcgcctgc gtttcctgtt gcttgtgcgc
     2461 cttctgctcc ttcttgagct tgttgcgctg ctgcggcttg ttcgtagagt ttcacagcaa
     2521 ggccttgagc tttttcgttc aacgcttcaa gtttcgcttt catgtcgtcc aagttgttgt
     2581 cttcttgagc tttcttaagg tcatcaaggg cagcttgggc agcgtcacgt tctgcgtcga
     2641 agcctttacc ttcagtttcc ttgattgtct tttcagtcgc aaagattgct tggtctactt
     2701 cattacgaag gtctacttct tctttacgtt tcttatcggc ttcagcgttt gcttctgcat
     2761 ctttcatcat gcggtcgatt tcttcgtcag tcaaacctga gttcgattgg atgacaatag
     2821 tttgttcttt ttgagttcca aggtctttgg ccttaacaga cacgataccg ttcttgtcga
     2881 tgtcaaatgt tacttcgatt tgaggaattc cacgaggtgc agctgggata tcagtcaatt
     2941 ggaagcgtcc aagagtcttg ttatctgctg ccattgggcg ttcaccttga agaacgtgga
     3001 tatcaacggc tggttggttg tctgctgctg ttgagaagac ttgtgattta gatgttggga
     3061 ttgtagtgtt gcgatcgata agttttgtaa atactccacc cattgtttcg ataccaagtg
     3121 acaatggcgt tacatcaaga aggacaacgt ccttgacatc accagtaatc acaccacctt
     3181 ggatagccgc acccatagca actacttcat cagggtttac tgatttgttt ggttctttac
     3241 cagtttcagc tttaacagct tcaacaacgg cagggatacg agttgaacca ccaacaagga
     3301 taacttcgtc aatttctgac aagctcaaac ctgcatctga aagggcttga cgaactggaa
     3361 cttttgtacg ttcaacaagg tcacgagtca aatcgtcaaa tttcgcacga gtcaaagtca
     3421 tttccaagtg aagaggtcca gcctcacctg cagtgataaa tggcaagctg atttgtgttg
     3481 aagttacacc agaaaggtct ttcttcgctt tttcagccgc atctttcaaa cgttgcattg
     3541 ccatcttgtc agtagacaag tcgataccgt tttctttctt gaattctgct accaagtggt
     3601 caatgatttt ttggtcaaag tcgtcaccac caagtttgtt gtcccctgca gttgacaata
     3661 cgtcgaagac accgtcaccc aattcaagga tagagacgtc gaatgtacca ccaccaaggt
     3721 caaataccaa gattttttct tctttgtcag tcttgtccaa accataagca agagctgctg
     3781 cagttggttc gttaacaata cgttctactt caagaccagc aattttacca gcgtcttttg
     3841 ttgcttgacg ttgagcgtcg ttgaagtaag ccggaactgt gataacagct ttggttactt
     3901 tctcaccaag gtagtcttca gcgtagcctt tcaagtattg aaggatcata gctgagattt
     3961 cttgtggagt gtattctttt ccatttgcag aaactttttc agaagttccc atcttagatt
     4021 tgatagagat aactgtatct gggtttgtga ctgcttgacg ttttgcagca tcaccaacga
     4081 tgatttctcc gtttttgaat gagactacag atggagttgt gcggttccct tctgggtttg
     4141 cgatgatttt gctttcagtt ccttcaagaa ctgcaactgc tgagtttgtt gtacctaagt
     4201 caataccgat aattttagac atgtgttttt ctccttgtta gttaattttc tatttttctt
     4261 tttgatactc ttcttaagtg gcgacgccgt cagagcttga cttcgtcaat ctctttgact
     4321 aaactttgag cctaaggtct caaaatttgc gcaaatagca ccacggcgaa gagtatcgtc
     4381 tttggttcgc ttcgctcact attacaaagc caaacatatt ttatctttct tcatagttta
     4441 ttgtcgtttc ggacaagttt tctattatgt aaattgcgac acgaggagtc gaaatcgatt
     4501 ttatttcgac gacgagttag taaggaagct aggcaaacgt catagcgatt gccgttttct
     4561 gacgagttgc tttagctacc gtcagaattg ccgaatcgaa cacgccctaa gctctgagta
     4621 aaaaagatag attttcttgt gttcatcgaa cacttcgtca atctcctatt tttactgcga
     4681 gctttttacg agctttgtat cttagttata caccactacc attgctgggc gtaggatgcg
     4741 gtcatggagt ttgtagcctt tttgaaagac ttgggcgatg gtatctactg ggtgttcatc
     4801 gtctgctggg agagtttgga tggccatatg gtagttatgg tcaaattcgc catctgctgc
     4861 gatttcttca attccttctt ctttcaaagc gtgaatcaag ctttcttgca ccatcgccaa
     4921 gcccttcttc acatcatctg tcaaaccttc aactgcaagt gcacgctcaa ggttgtcaag
     4981 agatggtaag attgcttttg ccaagtcctg gctacgataa cgttgcaagt tttgacgttc
     5041 ttcattggca cggcgttgga tattttgcat ttctgcatga gcgcgaagat atttgttttc
     5101 gaactcatct gcacgttcat ttgccaagtc caactcagac ttttcaggag ttgtttcttc
     5161 agctgtttcc acaacttcct cttcttgaac ttcttctact tcttcatttt ttatatcttg
     5221 ggccatgttc gcctccttta atgatttcaa tcttaatgta cttcgtaatg attactgctg
     5281 aggtagcggt aaaaatctgt caacttcatg gtcaaaacac ggttgaccac attgacttga
     5341 ttgattagct gttggtaatc cagattaact ggaccgataa tggctagaat tccaactccc
     5401 cgataaggaa tgaggaactt actactaatc accgctaggt cagctaaaca ggactcttga
     5461 ccgtctgcaa cacgaacatt ttgcatctga tcctcacgca acccctcacg aatctccaag
     5521 gccacctttt gcggttggtc aaagaactga taggctgcta gattggcaaa attcaagaga
     5581 tggaccttgc ccgccatcac aatgttttcg ttgaacattt ccttaaagat gtgttcaaag
     5641 agatcgatga cattatccgt tgttgtaaag taacgctgga taatctgcgg aatctccgtc
     5701 cgaatcttgt agtgaatatc taaaacggtg tgaccgagga aacgttcctg aatgatgctc
     5761 ttcagtttca gcaaatcctc ctgcaagaag ttccttggaa tcagaaactg actagtaacc
     5821 gttcgcgact cgtctagggt aaataccgcc aaggctgtat gttgccccaa aacaacgata
     5881 tcaaaggctg tcaaacgttg cctgctcggc tcaacatcca gtgccactac cgtacagcca
     5941 ctcaggtctg ttagcaagtt agcagcctct tgcagaatat cctccaattt gaagaattcc
     6001 tgatcaaagg ctttgacaat ctcatatacc tcattttcag ccagtcggtc aaaatccagt
     6061 gagtgtttca catagtactg aaaaccagca acacttggca tccgaccgct tgaagtatga
     6121 gccttctcaa gcaacccttg tttttctagt tccgccatgt cattacgaat ggttgcactg
     6181 ctagagttaa tagactcttg caaggttttt gatccgacag gttcgtgcgt tttggtaaag
     6241 atgtcaataa tcagatttaa aatatcctgc tgacgctctg taaccatctc atcactcctc
     6301 tctgtctgat caattagcac tcaatgcatc caagtgctaa ctcatgattc tattatacac
     6361 ccttagaaga gaatgtcaag acaaaaactc aaaaaattag cactctttta tcaagagtgc
     6421 taaaaatcaa tctgaagacc tagaaccttg ctattcatct actcggtatt tcctttttag
     6481 tctgaaataa ccatgaatta gataagaaat cagaaaagca tataaaacaa aaaataagaa
     6541 agaaagacat agaaagatcc tctctaaaca aactcataca aacaacaaga ccacctaaga
     6601 aagtagctgt acaggaacga agtctagatc gcataacttc ccatttgagc cccttactca
     6661 tatagacttg atcctctgga agtaaatcag aagacgattt acgaagaatc gaacaagaac
     6721 ctgctcctat caattcccaa cctctatctc taaaatcttg cagttcacgc ttatgctctt
     6781 taagaaatct agaatcatag atacgataga taacatcgtc cggttgacct tgctcaaaat
     6841 agaacaaacc aaaacgactc gttctatacc tccaaccttt caaatgcatc tcatgtaaat
     6901 attcttcttt cttgtccaaa tcaacaatgg tgaaaatccg aaattctact ctgctattca
     6961 ttgtcttacc ccaaaattag aaaacatgcc tggcgttatt tattagataa ttctttccac
     7021 ttttgactca atctccaaaa aatataagaa atctgaatcg caaaaactat caataaaacc
     7081 caatctatta tgaaaatcaa aaacactttc caactgaaag aactacctcc agtaacaaac
     7141 tttgagaaaa acggtagtag agctaaaaag agaagtaaaa taggaagcat ccgcattgtt
     7201 aaaatccgtt tggcataaaa aaatctttat ttaaactaaa atattatagc aaaatttacg
     7261 ccagtttttg aacggctgat gtagatattt tatactttca aaatgtttaa atgtgattat
     7321 ttatttttga aaaatagctc accagcccga ctgaaagtgc ttatagaatg ataataagtc
     7381 gcctgccgaa aacagcggaa aatagcggtg ttatgcggag ataatctgac gcgatgcgaa
     7441 agtatattgc atacttattt tcaacaattt agcagagtat ttttataagt gtgatataat
     7501 agaagtataa tttgttctga tagtttattt tatggagaag tagattttta gaatgcggag
     7561 ggttcaatat ggttgagttt ataaagtcta agaaagaaat gagtgaggag gatattaaag
     7621 caaatttcat cactcctgct attgtatcca aaggatggaa aaatggtgag catatcgctt
     7681 acgaagaata cttcactgat ggtcgaattg aagttagagg agataaggct cgtcgtaaag
     7741 aaggaaaaaa atcagactat tcactgtatt accaatttgg aactcgaatt gcaattgttg
     7801 aggcaaagga taataaacac agcgttcgag caggattaca acaagctatt gaatatggag
     7861 agattttaga tgttccattt gtttattctt cgaatggtga tggctttatt gaacacgacc
     7921 gtatcacgag agaagaacgt gagctggagt tagacgaatt ccctactcgt gaagaattat
     7981 tttctcgtat gacgaaggaa aaaggattga cgtacgaaat tacagaagct atctcaactc
     8041 catactatac agacgccttc tcaatgaaaa cgccacgcta ttatcagcaa atagctatca
     8101 accgtactat tgaaacagtt gccagaggac aaaaacgagt aatgtttgtg atggcaacag
     8161 gaacggggaa aacgttcatg gcttttcaaa ttattcatcg ccttcgaaaa gctggtttgg
     8221 ctaaacgagt tttattctta gcagatagaa acatcttagt agaccaaacg atggctgaag
     8281 actttaggcc attcgaaaag gtaatgacga aaattacacc aaaacttttg actgctcctg
     8341 aaaaattaaa ttcttttgaa atttatctag ggctttatca gcaactaact ggtgaagatg
     8401 gaactgaaac acattatcaa aaatttgaca aagacttctt tgatttaatc gtaattgatg
     8461 aagcacaccg tggttcagct aaggaaaaca gtaactggcg taaggtaatt gattatttca
     8521 gttctgcgac acagattggg atgaccgcta ctcctaaaga aaccaagaat gcttccaata
     8581 cggaatactt tggtgagcca atctatactt atagtttaaa acagggaatc gaggatggtt
     8641 ttttggctcc atatcgtgtt atgagggtta atttagatgt ggatgtggat ggttatcgtc
     8701 cagaaactgg aaaagttgat gctaacggac aattaataga agataggtac tacggcagga
     8761 aagattttga taaaaccatt gtcattgatg atagaacgca aagagttgcc aagtttgttt
     8821 ctgattatat gaagcaaaac aatgcacgat ttgataaaac aattgttttt tgtgttgata
     8881 ttgaccatgc cgagcgaatg cgtgctgcac ttgtaaaaga gaatctagac ttagtccaag
     8941 aagactatcg ttatgtcatg caagtaactg gtgacaacgc tgaaggaaaa gctcaactgg
     9001 ataactttat ggatgtcaat tctaattttc ccgctattgt aacaacgtct aaattattaa
     9061 cgacaggagt taatgctaaa acatgtcgtt tgattgtttt agactctaat atccaatcca
     9121 tgactgaatt taaacaaatt attggtcgtg gcacacgtct ttatcctcaa aaggggaaag
     9181 aattttttac gattattgat tttcgaaatg ttaccaattt gtttgctgac cctgattttg
     9241 atggtgatcc agtgaaggtg ctggaaacag gtgcgaaaac agtcagtggt tctacgcccg
     9301 gtttcgtaga tgaggaaggt gacccagtag aaaaatatat cgttacagac aagcaggtta
     9361 ccattcttaa ttctactgtt caagtattgg atgaaaacgg gaaactgatt accgaaagcc
     9421 tgaccgacta cactcgaaag aatatcttag gtagctacgc cactttgaac gattttatca
     9481 cagtttggca tacggcagat aaaaagaagc ttatcttaga cgaactttat aaaaaaggag
     9541 tttatctaga tgctattcga gagtcggagg gaatatcaga acaagaaatc gatgattttg
     9601 atttactcct aaaacttgcc tatggtcaaa aagaattaac caaaacggaa cgtatcaata
     9661 aactcaaaca aagcggatat ttatataaat atagtgagga agcgcgtgct gttttggaaa
     9721 ttttactgaa caaatacatg gataaaggta ttggagaact cgaaagcatt gaaacattaa
     9781 aacttccaga atttcagata tatggtggaa ccttcaaaat catcaatact tattttggag
     9841 ataaaaaacg atatttacaa gcaattaaag aattggagca agagctattt acagtagctt
     9901 aatgaaagga aagtatgtca attacatcat ttgtaaaaag aattcaagat atcactcgaa
     9961 acgatgctgg tgttaatggt gatgctcaac gtattgagca aatgtcttgg ttattattct
    10021 taaaaattta tgatagccgt gaaatggttt gggaattaga agaagacgag tatgagtcaa
    10081 ttatcccaga ggaattaaaa tggcgaaatt gggctcatgc tcaaaatggg gaacgggtat
    10141 tgacaggcga tgaattactt gattttgtca ataacaagtt attcaaagag ttgaaagagc
    10201 ttgaaataac ttcaattatg cctattcgaa aaacgattgt taaatcagct tttgaagatg
    10261 cgaacaacta tatgaaaaat ggcgtcttgt tacgccaagt catcaatgtt attgatgaag
    10321 ttgatttcaa tagccctgaa gatcgtcatt cgtttaatga tatttacgaa aaaattctta
    10381 aagatattca aaatgctggg aactcaggag aattttatac gccacgtgca gcgactgatt
    10441 ttattgccga agttcttaac ccaaaacttg gagaatcaat ggcagacctt gcttgcggaa
    10501 caggaggctt cttgacttcg actctgaacc gtttaagtag tcaacgtaaa actagtgaag
    10561 ataccaaaaa atataataca gctgtttttg gtattgaaaa gaaagcattt cctcatcttt
    10621 tagcagttac aaatctgttt cttcacgaaa ttgatgaccc taaaattgtt catggaaata
    10681 ctttggagaa aaatgttcgt gaatatacgg atgatgaaaa atttgacatt attatgatga
    10741 atccaccttt tggagggtca gaattagaaa caataaaaaa taactttcca gcagaattac
    10801 ggagttctga aacagctgat ttatttatgg ctgtcattat gtatcgtttg aaagaaaatg
    10861 gtcgtgttgg agttatttta cctgatggtt ttctatttgg tgaaggtgta aaaactcgct
    10921 tgaaacaaaa actggtagat gagttcaact tgcatacgat tattaggttg cctcatagtg
    10981 tctttgcacc gtatacagga atccatacga acattctttt ctttgataaa acaaagaaaa
    11041 cagaagaaac ttggttttat cgtttagata tgccagatgg ttataaaaat ttctcgaaaa
    11101 ctaagccgat gaagtcagaa cacttcaatc ctgttcgtga ctggtgggaa aatcgtgaag
    11161 agattctgga aggtaagttc tacaaatcta aatcatttac acctagtgaa ttggctgagt
    11221 tgaattataa tttagaccag tgtggttttc caaaagagga agaggaaatc ttaaatccct
    11281 ttgagttgat tcagaattat caagcggaaa gagcaacttt aaatcataag attgataatg
    11341 tattagctga tattttgcag ttgttggagg acaaataatg acaccagaac aacttaaagc
    11401 aagtattctc caaagagcga tggaagggaa attagtgccg caaaatccca atgacgaacc
    11461 tgcaagtgaa ttattaaaga gaattaaagc tgaaaaagaa aaacttatca gtgaaggaaa
    11521 aatcaaacga gataaaaagg aaactgagat atttcgtggt gatgatggga aacattatgg
    11581 gaagtttgct gatggaagta ctcaagaaat tgatgttcct tatgatattc ctgatacttg
    11641 ggagtgggtg aggataaaat caatttattg gaattttggg caaaataagc cagagaaatc
    11701 ctttaggtat atagatacgt ctagtattga tagaaaaaag aacataatca actacaaaaa
    11761 tctacaatat ctttcacctg aacaagcgcc ttcccgtgct agaaaattag tttcgcagaa
    11821 tagtgtctta ttttcaacag ttagaccata tctaaaaaat attgctgtag ttagagaact
    11881 taaagagtat ttgatagcta gtacagcatt tattgttttg
//
LOCUS       DAVKLW010000060        11854 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKLW010000060 DAVKLW010000000
VERSION     DAVKLW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.060"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..674
                     /locus_tag="VW324_001708"
     CDS             <1..674
                     /locus_tag="VW324_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU9727288.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
     gene            1358..1519
                     /locus_tag="VW324_001709"
     CDS             1358..1519
                     /locus_tag="VW324_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727289.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1611..3290
                     /locus_tag="VW324_001710"
     CDS             1611..3290
                     /locus_tag="VW324_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9727290.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEERQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3558..4124
                     /locus_tag="VW324_001711"
     CDS             3558..4124
                     /locus_tag="VW324_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727291.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4446..4634
                     /locus_tag="VW324_001712"
     CDS             4446..4634
                     /locus_tag="VW324_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9727292.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4696..5454
                     /locus_tag="VW324_001713"
     CDS             4696..5454
                     /locus_tag="VW324_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9727293.1"
                     /translation="MVVYIRQSKLLSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIDVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            5512..6366
                     /locus_tag="VW324_001714"
     CDS             5512..6366
                     /locus_tag="VW324_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9727294.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKDYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6478..7362
                     /locus_tag="VW324_001715"
     CDS             6478..7362
                     /locus_tag="VW324_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9727295.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7525..9264
                     /locus_tag="VW324_001716"
     CDS             7525..9264
                     /locus_tag="VW324_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU9727296.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIWKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            9539..9994
                     /locus_tag="VW324_001717"
     CDS             9539..9994
                     /locus_tag="VW324_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727297.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSENLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            10439..10915
                     /locus_tag="VW324_001718"
     CDS             10439..10915
                     /locus_tag="VW324_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9727298.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            10915..11676
                     /locus_tag="VW324_001719"
     CDS             10915..11676
                     /locus_tag="VW324_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9727299.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT         4288 a         1637 c         2449 g         3480 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata
      481 aaaatctaaa agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat
      541 acatggaaag cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg
      601 aagaagataa agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag
      661 gagaatcttt atagataaaa agaggtggaa aggcactatt aaagacttta agtttaaaaa
      721 ggtatcaaag tatgccagag ataaaaaaat ataaatatga cactttaaag gcgattagaa
      781 aattaaagaa atgctagaag acttttagat gacagttacc aagagggtct aattttcact
      841 gctacttatg cagtaggaat tggacctttt tatataagca aattctattt caaaattggt
      901 taccaagaaa gacttctttt cactgttaag tataagaggg taatttatag aattttgtta
      961 catcaagata aatattttct gatggtcaaa ttttaaaaag attaataact ttcgataaat
     1021 tttgaacctt aacaattgaa tagaccaaga caagacgtta attatttttg gagcgtaata
     1081 acctatccgt caagatcttt cggctgaata attcggcaaa aaaagttctg ctaagcaaaa
     1141 agaaagtgag cgataagtaa gagtttgaat atagggaggg ataccctatc cgctatgaag
     1201 aaaatgagga taatgatact tctaaggttg aagccggctc atccggaata gaggcggagg
     1261 tgagattcct atgttgctaa tccaaagcac ttgtcaatgt caaaaaactt aattaaacaa
     1321 ataattatta gatataaaac tttagaggag gtatatattg gacaatgact ggaatggatt
     1381 ggctgattta atagcaaatc tgattgcaaa atatgctggt gctttagatt tagataatct
     1441 acccgatcca acgccagcaa aaaatcaaga gatgaaaaat agttttgaca tggcaaaaac
     1501 acaaattgag acggactaat aaaagtgtta taatatactt gaaatgatag tccaaactta
     1561 aattaaaaaa attatatatg atatagaaaa attatattga ggttaatact atgtcaaaag
     1621 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
     1681 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
     1741 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
     1801 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
     1861 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
     1921 attatggagt taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga
     1981 aactaatgat ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc
     2041 aaacaatgga aggttgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc
     2101 cgtatggata taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa
     2161 taagaacgat tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat
     2221 acttagagaa tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg
     2281 atgcaggtct tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg
     2341 gaagaagaac tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag
     2401 atgaatatct aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag
     2461 ctgctcaggt taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag
     2521 atacacgcac acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt
     2581 ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact
     2641 atggttgtaa acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta
     2701 gagaagaatt gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga
     2761 aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa
     2821 aagaaataga taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa
     2881 ttgaggaaat agataattta gatgttgaag ataagcatta taagagaagg aaacaggatt
     2941 tagatgatag actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg
     3001 atgcgaaagc aaagaaacag actattgaag ctgaaaagct tacaggagat aacatatata
     3061 aggttctaat ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt
     3121 taatttcagc tttgatttct gaaattcaag tttatgaaga aagacaatct aacggacaat
     3181 ggctaaaatc cattactttt aagctaccaa ttatagaaga aaatctcaac ataggtttgg
     3241 acaatgatga acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag
     3301 gttccccgaa ccttttgagt cccactgacg catcacactg aggcggtatc actgcttgta
     3361 cgagttgaag catcagcaaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg
     3421 aaggtcgata aggtgtgttc tgggtataag actcgtaaag taagtaggaa atatgatgag
     3481 atgatacaga gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga
     3541 aaatggagag attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt
     3601 tagtagcatt tgattccata gaagatggga aagcctttgt agctcaaatc ccgggataca
     3661 ctctggaaac agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt
     3721 atatggaaat tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg
     3781 aggaaaatgt aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag
     3841 tgatcgaagg atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct
     3901 atgtcgaaac aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat
     3961 atgaaatcga cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa
     4021 aagggatcga agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag
     4081 atgtagaagg atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt
     4141 gtgagtatat acctttgcta gtaaaagagt caaaacgtta taacgaaggg ggggaagaaa
     4201 acccttgagt gtaagtcttt ttcttgttta gagggaaagt attagagtag ccgctcataa
     4261 tttatactag atttgataac tgatttgaga cagattttat ttctataaat attgtgaaaa
     4321 ttttttaata ccaaagattt aaagttactg tgttaaaaat cctctccact tttaaactaa
     4381 tgtattataa aagccagttg acacaagtgt tacacctgtg atagaattaa cttgaggtga
     4441 tgaaaatggc aaccgataaa aatagaataa tgattagctt ggatgataaa aatctagaga
     4501 aacttgaaaa tttagtagaa gatgctagag atagaagggg aatgcgacta acaaagtccc
     4561 aggttattga attactccta aataccgttg attatttcga tgatattatg ggggctattt
     4621 actcaaaaaa ataagagcaa aattgctatt attttttaca aattggtgta acactagtga
     4681 tataggagtg atgaaatggt tgtttatatt agacaaagta aattactaag tgaggtttca
     4741 attaataaat acaatgctca agtaggtgcc tacttacaag gagaagaagt tattttatat
     4801 caatcttttt ctgaaataaa agagttaaca agtgaagata tagatgtaga ttatataatg
     4861 gagactagag cattactaaa aatgatgggc ttaaacgttc cggtttatga ctatcctatt
     4921 gagcttaaag agttttatgg tcgaaagatt tatgctggta ttttaggaga gattgtgaat
     4981 atacctgata attggggaaa atttattaag cctaaagctg gttcaaaagt ctttactgga
     5041 agagttgtta atggaaccca tgatttaata ggtattggtc tacctttcga ctatcctata
     5101 tggattagtg aggttgtaga attcatagct gaatggcgtt gttttgtgtt agatggtcgc
     5161 gtattagatg ttcgacccta tacaggtgat tatcatgcac aatttgatgc aagtgtaatt
     5221 gatgaagcga tatcatgttg gaaagatgcg ccaatagctt atggactaga tatcggtgtt
     5281 actcgcgatg gcagaacact tgttgttgaa gtaaatgatg gttatgcatt gggaaattat
     5341 ggcctatctc ctttaaaatc aatcaatttt catagagcta ggtggaaaga aatggtaaaa
     5401 ccctattttg aaaaaaatga aatttttaaa atccaacagg atgttatttt ctaaaataaa
     5461 tatggtagat aaccaatata aaaagcatca aaatgaacaa ggaggaatct gatgcttgga
     5521 gcaattattg gagatattgt cggttctgtt tacgaatgga acaatatcaa aacaaaggac
     5581 tttcctttat ttcggaagga ctgctttttt acagatgaca cggttatgac ctgtgctgtt
     5641 gcagaagcaa ttatgaatgg tggacaaaaa gacgacttca ttgacgcgat gaagaaatat
     5701 ggcagaatgt atccgaatgc tgattacggt gctcggttta atgcgtggct aaacagcgat
     5761 aaccgtgagc cttataatag ctttgggaat ggatcagcta tgcgtatttc tccatgtgct
     5821 tggatcatgg actgtggttt ttatgcgaaa actggtatat ggccatcatc tagaggactt
     5881 acgagtcttt ctgcagaggt aactcataat catccagaag gtgtcaaagg cgctatggct
     5941 acagctgatg ctatctttct atgtcgtttt tactttggtg gttattgtag agagtacgag
     6001 caatcaatta acgacaatcc tacagagtgt aaaagacgaa ttaaggatta tatagaaaaa
     6061 gaatacgatt acaatctatc tcaaacttta gatgaaatcc gtcctaacta tcgttttaac
     6121 gaaacatgtc aggaaactgt ccctcaagcc attatcgcct ttcttgaaag tagagacttc
     6181 gaagatgcga taagaaatgc catctcactt ggtggcgaca gtgatactct cgcagcaatc
     6241 acttgtagca tagcagaggc agcttacggt attcctgatt ggatcaagga taaggactat
     6301 tcttacttgg atgaaccctt aaaggatgta gttaggcgat gggaaaatag aataaaagcg
     6361 tattaatctc cagtacgtca gatttctctt ttctgtattt tcattgtata ataggattac
     6421 atttacttgc atacgacgaa atgtaaacat ttctgtttaa aattaaggat aaaatcaatg
     6481 aaaaaagcaa tggtaattat caaccctact tctggtggcg agaaggcttt ggattacaaa
     6541 gaaaagctgg agaataaagc aaaagaatac tttgaatatg ttgaaaccaa aattaccgaa
     6601 aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc gtgagcagta tgatgcagtg
     6661 gttgtgtttg gtggagatgg aactgtcaat gaagtcattt caggtattga tgagagagac
     6721 tacattccta agttagggat tatcccaggc ggtacgggta acctcattac aaaacttttg
     6781 gaaatcaatc aagacatcga tggcgcaatt gacgaactgg attttgattt aaccaataag
     6841 attgatatcg gtaaagcaaa tgacaactat tttggttata tctttagtat cggttctctg
     6901 cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa aattcggtat tttaacctat
     6961 gctgtaaata ccatgaagtc tgtcatgaca gatcaggtct ttaacattaa ggttgagaca
     7021 gaaaatggaa attatgttgg tgaagctagc catgttttgg tccttttgac aaattacttc
     7081 gctgataaga aaatctttga agaaaacaag gacggctatg ccaacatttt gattctgaaa
     7141 gatgcctcta tattctccaa attatccgtc attcctgatt tattaaaagg ggatgttgtc
     7201 gcaaatgata atatcgagta tatcaaagcg cgtaatatta aaatctcttc agatagtgaa
     7261 ttggagtcag atgttgacgg agataaatca gataacctac ctgtagaaat caaagtccta
     7321 gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt agtatataga gaaagccttt
     7381 tttaaggctt tttgtatact ttaaaagata gttcctttaa caacggacat tccttgcaaa
     7441 tagtttacaa aaatagtata ctggattcat tgagcttgaa aacgtttgcg taaaatttga
     7501 atgaatactt taggagacaa attgatggaa ttgagtgcta tttaccatag gcctgagtcg
     7561 gagtatgcct atctttataa ggataagaaa ctccatattc gaattcgaac taagaaaggg
     7621 gacattgaaa gcatcaactt gcactatggg gaccctttta tctttatgga ggagttttat
     7681 caggatacaa aagaaatggt caagataact tctggtacct tatttgacca ttggcaggtt
     7741 gaagtgtcag ttgactttgc acgtatccag tatctctttg agctcagaga tacagaaggt
     7801 caaaatattt tgtatggcga taaagggtgt gtggaaaatt ctctagaaaa tcttcatgca
     7861 attgggaatg gatttaagtt gccttatctt catgagattg atgcctgcaa ggttcctgac
     7921 tgggtttcaa atacggtatg gtatcagata tttcctgaaa gatttgccaa tggcaatgct
     7981 ctattaaacc cagaagggac tttagactgg gattcatctg tcacacctaa gagcgatgat
     8041 ttctttggtg gtgatttaca ggggattatt gatcatatgg attacttgca agacttgggt
     8101 attactggac tatatctttg tcccatcttt gaatctacaa gcaatcacaa gtacaatacg
     8161 acagattact ttgaaattga ccgtcatttt ggagacaagg agacctttcg ggaactggtg
     8221 gatcaagcgc atcatcgtgg catgaaagtc atgctggatg cggtatttaa tcatattggt
     8281 tcgcaatctc ttcaatggaa aaatgtcgtc aaaaatggtg aacagtctgc ttataaggat
     8341 tggttccata ttcaacaatt cccagtgaca actgaaaagc tagttaataa gagagactta
     8401 ccctatcatg tttttggttt cgaggactat atgcctaagc taaatacagc caatccagag
     8461 gtcaagaatt atcttttaaa ggttgcgact tattggattg aagagtttaa tatcgatgct
     8521 tggcgtttgg atgtggctaa tgagattgac catcagttct ggaaggattt tcgtaaggca
     8581 gttttagcta aaaatcctga tctttatatc ctaggagaag tctggcatac atctcagcct
     8641 tggctaaatg gagatgagtt ccatgccgtc atgaattatc ctttatctga tagtatcaag
     8701 gactatttct tacgaggaat taagaagaca gaccagttca tcgatgaaat caatggagag
     8761 tctatgtatt acaagcagca gatttcagag gtcatgttta atctcttgga ttcacatgat
     8821 acagagcgaa tcctgtggac ggccaatgaa gatgttcaac tggttaaatc agccttagcc
     8881 tttctctttt tacaaaaagg aacaccgtgc atttattacg gaaccgagct agccttgact
     8941 ggaggaccag atccagattg tcgtcgttgt atgccttggg aacgtgtatc aagtgacaat
     9001 gatatgctga actttatgaa gaggctgatt aagatttgga aatacgcgtc agtaatcatt
     9061 tcgcatggca agtatagcct tcaagaaatc aaatctgatc tagtagctct ggaatggaaa
     9121 tacgaaggac ggatcctcaa agcaatattc aaccaatcaa cagaagatta tcttttagag
     9181 aaagaagcag tagcactagc aagcaattgc caagaattgg agaatcagct tgtcatctct
     9241 ccagatggat ttgtgatttt ctaaaaacta gttgatgaag attatggtac atttcatatc
     9301 ttatatagta taataaggct agttactaaa cttgtaaagg agaacttaaa tgaattgtag
     9361 aggacatgaa acaagacaaa gaattgttag agattttgaa gtttagccta aagcacatat
     9421 taagctgtta gcaaatcaac aaaaacatag tgatgcagga gcaactattg aagatgaata
     9481 ttatgtattt atcgctgaga gtaaaattga tggcaagaag gaagttattc agtgttgcat
     9541 gggtgcggca agggattttt tagaactaat taatcacaaa gggctacctc tttttaatcc
     9601 gcttgtaggt gattctcatg taaataatag acaagaatat gacaatacag ggagtgaaaa
     9661 tttacaacct gaaaagtgga atgaaactgc aaagcagctt tataatgcta taatgtggtt
     9721 gattatttta tggaatgcta agccggatac acctttattt aattttaaag acgaagtaat
     9781 taagtataaa acatatgagc cttttgaaag cagtataaaa agagtaaata ctactataaa
     9841 gaatggtagt aaagggaaaa ctctgactga gatgattaat ggctacagag cggataacga
     9901 tattagagat gaaatttgta actttaatat tctgaaaaat aaaattcgtg atatgaaaga
     9961 ccaacaagga aatacaatgg aatcttactt ttagttattg ttgaattttg ggtattctat
    10021 aaaatatcct aattgagatt taaatagtag actatacaat atagttaaaa tatcagtaaa
    10081 aacaacactt tattgaggta ttggatacgc tttgctaata gcctaataat cacatgtgga
    10141 gtgttgctac aacgaaaaag gtgataatcc ttaatttcaa gctattttat aagcattttg
    10201 tctttgtaga taaaggcaat tttgacaata aaaatcctaa aaggtgaatc gttatagatg
    10261 tatttgtaga tattgtttgc gcatcgaaaa aattaataca agaataaata tttatagctc
    10321 tttaggtgac ttttatagaa gtaaagttta ggatagaaaa acaagaaata acgcaccatt
    10381 tttggtgcgt tatgcttttt tatgctataa tggatttata aaaataaagg agtttgctat
    10441 gattggaaag aacataaaat ccttacgtaa aacacatgac ttaacacaag acgacttttc
    10501 ccgaattgta ggaatttctc gaaatagctt gagccggtat gaaaatggaa ctagttcagt
    10561 ctctacggaa ctaatagaca tcatttgtca gaagtttaat gtatcttatg tcgatattgt
    10621 aggagaagat aaaatgctca atcctgttga agattatgaa ttgactttga aaattgaaat
    10681 tgtgaaagaa agaggtgcta atctattatc tcgactctat cgttatcaag atagtcaggg
    10741 aattagcatt gatgatgaat ctaatccttg gattttaatg agtgatgatc tatctgactt
    10801 gattcatacg aatatctatt tagtagaaac ttttgatgaa atagagagat atagtggcta
    10861 tttggatgga attgaacgta tgttagagat atctgaaaag cggatggtag cttaatggaa
    10921 atccaagatt atactgatag tgaattcaaa catgctttag caaggaatct tcgttcactg
    10981 acaagaggaa aaaagtccag taagcaacct atagcgattt tgcttggagg gcaaagtggt
    11041 gccggtaaga ctacaattca tcgtattaaa cagaaagaat ttcaaggaaa tattgttatc
    11101 atagatggtg atagttttcg ttctcagcat ccacactatt tagaactgca gcaagaatat
    11161 ggcaaagaca gtgtagaata taccaaagat tttgcaggaa aaatggtaga gtctttagta
    11221 acaaaattga gtagtttgag atacaatctt ttgatcgagg gaactttacg aacagttgat
    11281 gttccaaaga aaacagcaca actcttgaaa aataagggat atgaagtaca attggcctta
    11341 attgcgacaa agcctgaatt gtcgtatcta agtactctta tccgttatga agaactgtac
    11401 attatcaatc caaatcaagc acgcgcaact ccaaaagaac atcatgattt cattgtaaat
    11461 catctagttg ataatacacg acaattggaa gaactggcta tctttgaaag cattcaaatt
    11521 taccaacgag atagaagttg tgtatatgat tcaaaagaaa atacaacttc agcagcagat
    11581 gttcttcaag agttactctt tggggagtgg agtcaggtag agaaggagat gttgcaggtg
    11641 ggggaaaaga gacttaatga attacttgaa aaataaacaa ttgatatttt taggagaata
    11701 gaaatgagag ggtttaataa caagataaag tctgtttatc aagaactaac aaattccaaa
    11761 gagaaattcg gtagctttca caagacttta attcatttgc atacacctgt ttcttatgat
    11821 tacaagctat tttctaattg gactgcaacg aaat
//
LOCUS       DAVKLW010000061        11790 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKLW010000061 DAVKLW010000000
VERSION     DAVKLW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.061"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            96..749
                     /locus_tag="VW324_001720"
     CDS             96..749
                     /locus_tag="VW324_001720"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9727300.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            759..1223
                     /locus_tag="VW324_001721"
     CDS             759..1223
                     /locus_tag="VW324_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9727301.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1236..2504
                     /locus_tag="VW324_001722"
     CDS             1236..2504
                     /locus_tag="VW324_001722"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9727302.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2518..2778
                     /locus_tag="VW324_001723"
     CDS             2518..2778
                     /locus_tag="VW324_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727303.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2789..4150
                     /gene="pdrM"
                     /locus_tag="VW324_001724"
     CDS             2789..4150
                     /gene="pdrM"
                     /locus_tag="VW324_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9727304.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4304..5272
                     /locus_tag="VW324_001725"
     CDS             4304..5272
                     /locus_tag="VW324_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9727305.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5288..6280
                     /locus_tag="VW324_001726"
     CDS             5288..6280
                     /locus_tag="VW324_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9727306.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6498..7541
                     /locus_tag="VW324_001727"
     CDS             6498..7541
                     /locus_tag="VW324_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9727307.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHSYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7605..9290
                     /gene="lpdA"
                     /locus_tag="VW324_001728"
     CDS             7605..9290
                     /gene="lpdA"
                     /locus_tag="VW324_001728"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9727308.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9354..10343
                     /locus_tag="VW324_001729"
     CDS             9354..10343
                     /locus_tag="VW324_001729"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9727309.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10416..11486)
                     /gene="xerS"
                     /locus_tag="VW324_001730"
     CDS             complement(10416..11486)
                     /gene="xerS"
                     /locus_tag="VW324_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9727310.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3496 a         2210 c         2650 g         3434 t
ORIGIN      
        1 tagtcgattt attaaatctt tagataaact gtaaaaagtg gtttccatag ccactttttg
       61 ctataataga ggcagtaaaa atgaaggagt cggctatgga acactcgtct tggcatgctt
      121 tgattaaggc gcaattacct gagggttatt tcgggaaaat caatcagttt atggagcagg
      181 tctattctca ggggattatt tatccaccca aggaaaaggt ttttcaggct ctcttgacaa
      241 cactgcttga agaagttaag gtggtaattc tagggcaaga cccctatcac ggaccaggtc
      301 aagcgcaggg cttgagtttt tctgtacctg actctatccc agctccacca tccttgcaaa
      361 atatcttgaa agaattgtca gatgatatcg gagttaagaa atctcatgat ttgacagctt
      421 gggctgagca aggagtcttg cttcttaatg cttgtttgac tgttcctgct ggacaggcca
      481 atggtcatgc tggtcaaatc tgggagcctt ttactgatgc tgtgattcag gtggtcaatc
      541 atctagatag accagtcgtt tttgtactct ggggagctta tgcacgtaag aagaaggcat
      601 tagttaccaa tcctcatcac ttgattatcg aatcagccca tccaagtcct ttgtcggttt
      661 atagaggatt ttggggttcc aagccctttt ccaaggccaa tgcattctta aaagagacag
      721 gacaagagcc aatcgattgg cttagataag gagaaaatat gcctcagtta gcgacgattt
      781 gctacattga taatgggaaa gaactgctca tgctccatcg taataagaaa cccaatgatg
      841 tccatgaagg gaaatggatt ggtgtgggtg gtaagctaga gagaggagag acgccccagg
      901 aatgcgcggc gcgtgaaatc cttgaagaaa cagggctcaa agccaagcca gttctaaaag
      961 gtgtcatcac ttttcctgaa tttacaccag atttagactg gtacacctat gtttttaagg
     1021 tgacggagtt tgagggcgac ttgattgact gcaatgaggg gacgctagaa tgggttccct
     1081 atgatgaggt tttgagcaag ccgacttggg aaggtgacca cacctttgtt gagtggcttt
     1141 tagaggataa acccttcttt tcagccaagt ttgtttatga tggggataaa ttgttggata
     1201 cccaagttga tttctatgaa taaaggagaa agcagatgct actaatcaaa aatggtcgtg
     1261 taatggatcc caagtctggt ttggatcaag tttgtgatgt cttagttcaa gatgggaaaa
     1321 ttatcaaaat tgcgtctgag atcacggaag aaggagcaga aacgattgat gctactggtc
     1381 ttgtggttgc tcctggcttg gtcgatattc atgttcattt ccgtgaacct ggtcaaacac
     1441 ataaagaaga cattcatact ggtgccctag cagccgctgc aggtggtttt actactgtcg
     1501 tcatgatggc taataccagt ccaaccattt cagacgtgga gactttgcaa gcagttctcc
     1561 agtcagctgc caaagagaag attaatgtca agacagttgc gaccattact aaaaacttta
     1621 atggtaaaaa cttgactgac tttaaggcac tcttagaagc tggtgcggtt ggtttctctg
     1681 atgacggtat tccgcttgag agcagtaaga ttgtcaagga agccatggag gaagccaaaa
     1741 aactcaatac ctttatcagc cttcatgagg aagatccagg tttgaacggt gttcttggct
     1801 ttaatgaaaa tattgctaga gaacatttcc atatctgcgg tgctactggg gtggctgagt
     1861 acgctatgat ggcgcgtgat gtcatgattg cctatgcaac taaagcccat gttcacatcc
     1921 agcatttgtc taaggaagaa agtgttaaag tagtggagtt tgctcagggg ttaggtgcag
     1981 aagtcacagc agaagtagcg ccacagcatt tctctaagac agaagcactt cttttaacac
     2041 aaggtagcaa tgctaagatg aatccaccgc ttcgtttgga atcagaccgt cgtgccgtta
     2101 tcgaaggtct caaatcaggt gtcatcacag ttattgcgac tgaccacgcg cctcatcatg
     2161 tagatgaaaa aaatgttgag gatattacca aagcgccatc tggtatgact ggcttagaaa
     2221 catccctgtc tctcagcttg acctatttag tagaagctgg tgagttgagc ttgatggaat
     2281 tacttgaaaa aatgacatac aacccagcca agctttacaa ctttgaagca ggttacttgg
     2341 ctgagaatgg tccagcagat atcactattt ttgatgccaa ggctgaccgc tttgtggact
     2401 cccattttgc ttccaaagca gctaattcac cattcatcgg tgaaacctta aaagggcagg
     2461 ttaaatatac catctgtaag ggacaaatcg tctatcaagc ttgataggag ttagaatatg
     2521 aattattttc gaaaacgtag ggagagacaa gcgaaatcta atagtggaat ttactgtccc
     2581 gcagctaatc gttccaaaat tcaatatgaa actaaagaaa aagctgacca tgcggttcaa
     2641 tatgataacg gaggtttagt tagaagctat tattgtcgta catgtgcgtg ttggcatact
     2701 acttcaaaat ctaataaacc tccgctaagt ttgaaaaact atggtctgaa actctttggt
     2761 ttagataaat cagaatagaa agagaagtat gtataagaca aagtgtttac gagagaagtt
     2821 agtattattt ttaaaaattt tcttcccaat cctgatctac caatttgcca attattctgc
     2881 ctcttttgtt gatactgcaa tgacaggtca atacaacact atggacttgg ctggtgtatc
     2941 tatggcaacc agtatctgga atcctttctt tacatttcta acagggattg tgtcagcctt
     3001 ggtgcctatc attggtcacc atcttggtcg aggcaaaaag gaagaagttg cgtctgattt
     3061 ttaccaattt atttatttgg ccttgggcct atctgtggtc ttgctgggga tggtactttt
     3121 cttggcacca ataatcttga atcatattgg gttagaagca gcagtagcgg cagtagcggt
     3181 tcgctatctt tggtttttat ctatcgggat tatccccttg ttgctcttta gcgtcattcg
     3241 ttccttgctg gattcgctgg gcttgaccaa actgtccatg tacctcatgc ttttgttact
     3301 ccctctcaat agcggattta actatctctt gatttacggt gcctttggtg ttccagaact
     3361 gggaggggct ggtgctggtt taggaacatc cttggcctac tgggtcttgc ttgggatttc
     3421 tgttctggtt ttatttaaac aggagaagct caaagcctta caccttgaga aacgaattcc
     3481 acttaatatg gataacatta aggaaggagt tcgtttaggt ctgcctattg ggggaactgt
     3541 cttcgcggaa gtggctatct tttcagtggt tggcttgatt atggctaagt tttcgtcctt
     3601 gattatagct agtcaccagt cagctatgaa cttttcaagt cttatgtacg cctttcctat
     3661 gagtatctca tcggctatgg ctattgtcgt ttcctatgaa gtgggagcca agcgatttga
     3721 tgatgcgaaa acctatattg gtctaggaag atggactgct ctcatttttg cggccttcac
     3781 cctaaccttc ctttacattt ttaggggaaa tgtggccagt ctttatggta acgacccaaa
     3841 atttatcgat ttgacagcgc gttttttaac ttatagtctt ttcttccagt tagcagatac
     3901 ctttgcggca ccgcttcagg gaattttgcg ggggtataag gatacagtta ttccttttta
     3961 ccttggtttg cttggttatt ggggcgtagc aatccctgtg gctacgctat ttgattccct
     4021 aacagatttt ggagcttatt cttactggat cggcttgatt attagtttga ttgtgagtgg
     4081 ggcgctctac cgttggcgtt taactgtgat tatgaagaga tttgaatctt tagcaaaatc
     4141 caaacgctaa aaagtttgtg aaaaaatatt cttgataaga aaatcccttg ttttcacggg
     4201 gttttctttt ttattttgtg aaatttcttt tagaaaaact ttgacagtat ttgccaaaag
     4261 cggtaaaata gtaaggtaag aagaaagtta gaaaggcaag aagatgtcaa ctttagataa
     4321 aaatcttttg ctagaaatgt tccgtaagat ggaagaaatc cgtcgcatgg acttaaaaat
     4381 tgcacaatta gtaaagaaag ggaaagtgcc aggaatgacg cacttttctg ttggtgaaga
     4441 ggcagctaac gtgggggcta tgttagctct caatccagat gatctgatta cctcaaacca
     4501 ccgtggacac gggcaagcta ttgctaaagg gattgacctc aacggaatga tggctgaaat
     4561 ccttggtaaa tacactggaa cctgtaaagg gaaaggtgga tctatgcata tcgctgacct
     4621 tgatgctggg aaccttggtg ccaatggtat cgtaggtggt ggtatgggga tcgctgtcgg
     4681 tgcagccctc agtcagcaaa tgcaaaatac cggtaaaatc gttgtctgct tctttggaga
     4741 tggtgcgacc aatgaaggtg ttttccacga agcagtgaac atggcttcta tctggaacct
     4801 gccagtcatt ttctattgca ttaacaacgg ttacggtatc tctgcggata tcaagaaaat
     4861 gaccaatata gaacatatcc atcaacgtag cgccgcttat ggaattcctg gaatgttcat
     4921 cgaagatggt aacaatgtca tcgatgtcta tgaaggattt cagaaagctg tggaccatgt
     4981 tcgcagtggc aatggttcag tcttgattga aagtgtaact tatcgctggc tcggtcactc
     5041 atcatctgac cctggtaaat atcgtacgcg tgaagaagtg gaattgtgga aacaaaaaga
     5101 tccaatcgaa aacctccgca attaccttat tgaaaataac attgcaagtg ccgaagaatt
     5161 ggaagaaatc caagcgcaag taaaggaagc agtagaagct tctgttaaat ttgcagagga
     5221 aagcccattc ccaccgcttg aatcagcctt tgaagatatt tacgcagact aaggagaaag
     5281 agataaaatg gaaacaaaaa caatgtcctt ccgtgacacc attatccttg ctatgtctga
     5341 ggaaatgcgt cgcgatgaaa atgtgttctt gatgggagaa gatgtcggtg tcttcggagg
     5401 agacttcggt acttctgttg gaatgcttga agaatttggt ccagaacgtg tccgtgactg
     5461 tccgatttct gaagctgcca tctctggagc agcagcagga gcagccatga caggacttcg
     5521 tccaatcgtc gatatgacct tcatggactt ctctgttatt gccatggaca atatcgtcaa
     5581 ccaagctgct aaaacacgtt acatgtttgg tggtaaaggt caggttccaa tgactgttcg
     5641 ttgtgcagct ggtaacggag ttggctctgc cgcccagcac tcacaatctc tagagtcttg
     5701 gtttactcac attccaggac ttaaggttgt ggcacctgga acacctgcgg acatgaaagg
     5761 cctgcttaag tcttctatcc gtgataacaa cccagttatt atacttgagt acaagtcaga
     5821 atttaaccaa aaaggggaag tgccagttga tccagactac acaattccac ttggggttgg
     5881 tgaaatcaaa cgccaaggaa cggatgtaac agttgttact tatggaaaaa tgcttcgccg
     5941 tgtggttcaa gctgctgaag aattagcaga agaagaaatt tcagttgaaa ttgttgaccc
     6001 acgtaccctt gttccgcttg ataaggatat catcataaac tcagtgaaga agactggtaa
     6061 ggttattctg gtcaacgatg cccacaaaac aagtggctat atcggtgaaa tttcagctat
     6121 tatttcagaa tcagaagcat ttgactatct agatgcacca atacgccgtt gttcaggaga
     6181 agatgtgccg atgccttatg cacaaaacct agaaaatgca atgattccaa cagttgaaag
     6241 tatcaaagat gcaatccgaa aaacttataa caaagactag agttacataa tataagttat
     6301 gtaactaaaa taaagctgac gagaaacttt gtatctttta ggtgcagagc tcttcgtcat
     6361 taatatctta tattcgagca cgggctaaaa gctcggaaaa aagataaatc tccttggctt
     6421 catcgaagcc atcgtcgatt tcctattttt cagtcgcttt tgacgccctt tgtatcataa
     6481 atagaattgg agaggtcatg gctgatgaca agctaagagc gactcctgca gctagaaagt
     6541 tagcggatga tttagggata aacctctacg acgtttctgg ctcaggtgca aacggtcgtg
     6601 tccacaaaga agacgtggaa acttataaag acacaaacgt ggttcgcatt tcgccacttg
     6661 caaaacgaat tgccctcgaa cataacattg cttggcagga aatccaagga accggtcatc
     6721 gtggtaaaat catgaagaag gatgttttgg ccctgcttcc tgaaaatatc gaaaacgata
     6781 gcatcaaatc tcctgctcag attgaaaaag tggaagaagt ccctgataac gtaacaccat
     6841 atggtgaaat cgagcgtatt ccaatgacac caatgcgtaa ggttattgcc caacgcatgg
     6901 ttgaatctta cttgactgcg ccaaccttca ccctcaacta tgaagttgat atgactgaaa
     6961 tgttggctct tcgtaagaag gttcttgagc caatcatgga agcaactggg aagaagacta
     7021 ctgtaacaga ccttctttcg cttgcagttg ttaagactct tatgaaacac tcatacatca
     7081 acgcttcatt gacagaagat ggcaagacta ttatcactca caactatgtc aatcttgcca
     7141 tggcagttgg gatggataat ggattgatga cacctgttgt ttacaatgct gagaagatga
     7201 gtctttcaga actggtggta gcctttaagg atgttattgg ccgtaccttg gatggtaaat
     7261 tggctccaag tgagttacaa aattcaacat tcacaatcag taatttggga atgtttggtg
     7321 ttcagtcctt tggtccgatt attaaccaac ccaactcagc tatccttggt gtcagttcga
     7381 caatcgagaa gccagttgtc gtcaatggtg aaattgtgat tcgcccaatc atgagtttag
     7441 gattaaccat tgaccaccgt gtcgtagatg gtatggctgg tgctaagttt atgaaggact
     7501 tgaaagagtt gattgaaacg ccaatctcaa tgttaattta agagcaagag tattcatttt
     7561 taagatatag ataaaggaag gaagacatgg ccttagaaat aattatgcca aaagccggcg
     7621 tggatatgac agaaggacaa atcgtccaat ggaataaaaa agtcggagaa tttgtaaaag
     7681 aaggagaaat ccttttggaa atcatgactg ataaagtcag catggaattg gaagccgaag
     7741 aagatgggta cttgattgcc attctcaaag gagatggtga aactgtccct gtaacggaag
     7801 ttatcggtta ccttggtgaa gaaagggaaa acatcccaac agctggagca gcatcgccag
     7861 aagctagccc tgtacctgta gcaagtacct caaacgatga tggtaagagc gatgatgctt
     7921 ttgatatcgt tgtgattggt ggaggtcctg ctggttatgt tgcagccatt aaagctgccc
     7981 aactcggcgg taaggttgcc cttgttgaga aatctgaact tggtggaacc tgcttaaacc
     8041 gtggatgtat tccaaccaag acctaccttc atagcgctga aattattgaa aatatcggtc
     8101 atgctgcaaa tcgtggtatc gtgattgaaa atcctaactt cactgttgat atggaaaaac
     8161 ttttagaaac taaatctaaa gttgttaata ctcttgttgg tggtgttgca ggacttcttc
     8221 gtagctacgg agttgctgtt cataaaggaa ttggtacaat cactaaagac aagaacgtct
     8281 tggtaaatgg ttctgaattg cttgaaacca agaaaatcat tcttgctggt ggttcaaaag
     8341 tcagcaagat caacgtccct ggtatggaat ctccacttgt catgactagt gatgacattc
     8401 ttgaaatgaa cgaagtgcca gaaagccttg ttatcatcgg tggtggagtt gtcggtatcg
     8461 aactcggtca ggccttcatg acatttggtt caaaagtgac tgttatcgaa atgatggacc
     8521 gtatagttcc agctatggat gtggaagttt ctaagaacct tcgcttgatc cttgagcgta
     8581 aaggaatgac catcttgact ggtactaaac tgcaagaaat cattgaggaa aatggtcaac
     8641 ttcgtatcaa ggttgaagga aaagacgata tcatcgcaag caaagctctt ctttcaattg
     8701 gtcgtatgcc agaccttgaa ggtattggag aggttgagtt tgaattggat cgtggttgta
     8761 tcaaggtcaa cgaatacatg gaaacttcag ttccaggcat ttatgcacca ggtgacatca
     8821 acggtactaa gatgttggct cacgcagctt tccgcatggg tgaagtttcc gctgaaaatg
     8881 cccttaaagg aaatcatgca gttgccaaat tgaatttgac tcctgcagcc atctacactc
     8941 ttcctgaagt agcagcagta ggtttgacag aagaacaagc ccgtgagaaa tacgatgttg
     9001 ccatcggtaa gtttaacttt gctgctaacg gtcgtgctat tgcatctgac gcagcccaag
     9061 gtttcgtaaa agttatcgct gataagaaat acggagaaat ccttggtgta cacattattg
     9121 gtcctgcagc cgcagaatta ataaacgaag catcaagcat catcgaaatg gaaatcactg
     9181 ttgaggaaat gctgaagacc atccacgggc acccaactta ctctgaagtg atgtacgaag
     9241 cgtttgcaga tgttctagga atggccatcc attcacctaa gaaaaaataa aacgaagata
     9301 gattagactg gaaggttatt tccagtttct atcatataaa gtaaagggat atcatgaaat
     9361 acattatcaa tcattcaaat gacactgctt ttaatattgc cttggaagaa tacgccttta
     9421 aacacctttt ggatgaggat caaatcttcc tactttggat taacaagcca tctatcattg
     9481 ttggtcgtca ccagaacact attgaagaaa tcaaccgtga ttatgttcgc gaaaatggta
     9541 ttgaggtagt tcgccgtatt agtggtggtg gagctgttta ccacgattta aataacctca
     9601 actacacgat catctccaaa gaagatgaaa ataaggcatt tgacttcaag agcttctcaa
     9661 ctccggttat caataccttg gctcaactcg gggttaaagc tgagttcaca ggtcgtaatg
     9721 accttgagat tgatggtaag aagttctgtg gcaatgccca agcctatatc aacggccgta
     9781 tcatgcacca cggttgcttg ctctttgacg ttgatttgtc agtcctcgca aatgccctta
     9841 aggtttcaaa agataaattt gaatcaaaag gtgtgaaatc cgtccgtgcc cgtgtaacca
     9901 atattatcaa tgaattacca aaaaaaatca cagtcgaaaa attccgtgat ttactcttgg
     9961 aatacatgaa aaaagagtac ccagagatga ctgaatacgt cttttcagaa gaagaattgg
    10021 ccgaaatcaa tcgcatcaag gatactaagt ttggaacttg ggactggaac tatggtaaat
    10081 cacctgaatt taacgtccgt cgtggaacca aattcactag tggtaaggtt gaagtctttg
    10141 ctaacgttac tgaatcaaaa atccaagaca tcaagattta tggtgacttc tttggtattg
    10201 aagacgttgc tgcagtagaa gctgtccttc gtggggtgaa atacgaacgc gaagatgttc
    10261 ttaaggcgct aaaaaccatt gatatcacac gctacttcgc tggtattagc cgtgaagaaa
    10321 tcgctgaagc ggtagttgga taaatcaaaa agaagttggc caactaacca acttcttttt
    10381 ttgatattta tttacataat ttaaaatacg taaaatcata aactatccag agcattcttt
    10441 tgttcatcac taacaatatg ggtatagagg tcagtgactt gtgtgctagc atgtcctagt
    10501 tggtgactga ctaaaacttg tgatttagtc gcatcataga gcctagttgc tagtgtatgg
    10561 cgcagtttat ggggtgttac acgcacttta aaatcctctg agtatttagc aaccattttc
    10621 tcaacgctag aagcatcgat acgattagga acacctctgt agagagttaa aaaaagggct
    10681 gtatctgttt tttccgtttt atagcgttga ttccgaatgg ccagataatt ctctaaataa
    10741 ggtttagcaa aagcagcgac attgactgag tcacgtttgc caccttttcg agtaacatca
    10801 ataaccatca tttttagatt gagatctctt agatctagat taacagcttc agataagcga
    10861 acaccagatg ccaagagaag ggcaataatg gctaaatctc gttctttatt tttgttgaat
    10921 gatgagagag ctcgatttga aagctgttgt gggtgctctt gatcgatata agttagaaaa
    10981 ccttctgttt catcacctag aaagagtttt tgcttgatat tttcagctct ggcagcaagg
    11041 gtttctttct ttttcttggt tgaaactttt ttcattacat tacgatagaa ataaggttcc
    11101 ccctgatcgt tttcaacctc ctcggttaga tacttgtaaa gactagaaag tgctgataag
    11161 gttcgattga tagttgtctg tgaaacacct tgttttgttg tattagcatt cagcaaggga
    11221 cgttcacgta gataaaggat aaaggattcc atgtctttct tagacatatt ttccaaaact
    11281 gataaaggaa tatcggatat tttatcagcg tttgaaatac cagactccaa aacccagctg
    11341 aaaaatcggt catattcctt aaggtattcg tacagggttg taaaactgta gggcacagcc
    11401 agtttagatt ggtagtattc cagaacatac cagggcatga gttgttttag tttgtcgatt
    11461 cgttccagta aaatctcacg tttcatgtat tttctccata attaagtata ctagtagtat
    11521 agcatagact tgtatatttt tcaagaaaat tatagttttt ctgaaagatg ttataggagt
    11581 ttttcaaagt gataaaaaga cttagaccta caaacaaaac agaataagga aaataataaa
    11641 aatctcaccc actaaacttc tgttaatact acataactga agagctatat aaggagctat
    11701 gagatacaaa acacattgta taatagattg aaactagaat agtacatctg gacttctaaa
    11761 acattgctag aaattgattt gattgtcctg
//
LOCUS       DAVKLW010000062        11525 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKLW010000062 DAVKLW010000000
VERSION     DAVKLW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.062"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            720..1712
                     /locus_tag="VW324_001731"
     CDS             720..1712
                     /locus_tag="VW324_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9727311.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VW324_001732"
     CDS             1714..2532
                     /locus_tag="VW324_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9727312.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VW324_001733"
     CDS             2534..3319
                     /locus_tag="VW324_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9727313.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="VW324_001734"
     CDS             3417..3635
                     /locus_tag="VW324_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9727314.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3781
                     /locus_tag="VW324_001735"
     CDS             3620..3781
                     /locus_tag="VW324_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9727315.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
     gene            complement(4243..4317)
                     /locus_tag="VW324_001736"
                     /pseudo
     CDS             complement(4243..4317)
                     /locus_tag="VW324_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4550..10984
                     /locus_tag="VW324_001737"
     CDS             4550..10984
                     /locus_tag="VW324_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9727316.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         4027 a         1726 c         2310 g         3462 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat taatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta caaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cggtgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tatttcatga
      541 gagaaatcct aatttcacaa tctataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttctgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aacattttgt gaagattgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg agcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca aaagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctatc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttcg tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttaatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgtt gaggttttga
     3421 aggatttgcc agaatggttt ggaatcccag aaagcacaca agcctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaaggttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagg ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt tctcagctat ggaatccgta
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctattctcag
     3841 agtgccatac tgtaagtgta atcgccgatt tagctcagtt ggtagagcaa ggcactcgta
     3901 aagcctaggt tataggtaga taaacgactg aggagttgaa aaaatagata ggtagaagat
     3961 aaccgttaag ccttattctt agcggttatt tatattgttt aatagcgctg atatttcatc
     4021 aattatgcct gttttcgtgt ttctggtagt tgttcaagtt tattgctact atttttgatg
     4081 gtatgaatgt gcttataatg tatcccggtt aacgaaagtt ttggacttat actcatagaa
     4141 aatctcttca aaccacgtca acgtcgcctt gccgtgcgta tggttactga cttcgtcagt
     4201 tctatccaca acctcaaaac agtgttttga gctgactacg tcagttccat ctacaacctc
     4261 aaaacagtgt tttgagcaac ctgcggctag tttcctagtt tgctctttga ttttcattga
     4321 gtactaatct tattttttaa cgtgtgctaa aaaataaatt aatgcatgtt aatttttctt
     4381 gaattaaatt tttttaaatg atatactgtt tttatggaga gataacaatt atatataaat
     4441 gtactactct atttcctaga taatacttag taaaactttt tataacaaag gctagcaaag
     4501 ttaaagttta ttatctattg gaagttaaat aaatatgtaa ggaattaaaa tgaaaaaaag
     4561 tacagtattg tcattaacca cagctgcagt tattttagca gcctatgccc ctaatgaggt
     4621 agtcttagca gacacatcta gctctgaaga tgctttaaac atctctgata aagaaaaagt
     4681 agtattagat aaggaaacag aaaataaaga gaaacatgaa gatattcata atgctataga
     4741 aacttcaaag gatactgaag aaaagaaaac aacagttatt gaggaaaaag aagttgttag
     4801 taaaaatcct gtgatagaca ctaaaactag caatgaagaa gcaagaatca aagaagattc
     4861 caatcaatcc caaggagata atgcacactc gtctgcaaat aaaggcacag aaaatccgaa
     4921 aaaagaagat agacttgtct atattgctga atttaaagat aaagaatctg gagaaaaagc
     4981 aatcaagcaa ctatccaatc ttaagaatac aaaagtttta tatacttatg atagaatttt
     5041 taacggtagt gccatagaaa caactccaga taacttggac aaaattaaac aaatagaagg
     5101 tattacatcg gttgaaaggg cacaaaaagt ccaacccatg atgaatcatg ccagaaagga
     5161 aattggagtt gaggaagcta ttgattacct aaagtctatc aatgctccgt ttgggaaaaa
     5221 ttttgatggt agaggtatgg tcatttcaaa tatcgatact ggaacagatt atagacataa
     5281 ggctatgaga atcgatgatg atgccaaagc ctcaatgaga tttaaaaaag aagacttaaa
     5341 aggaactgat aaaaattatt ggttgagtga taaaatccct catgcgttca attattataa
     5401 tggtggcaaa atcactgtag aaaaatatga tgatggaagg gattattttg acccacatgg
     5461 gatgcatatt gcagggattc ttgctggaaa tgatactgaa caagatatca aaaactttaa
     5521 cggcatagat ggaattgcac ctaatgcaca aattttctct tacaaaatgt attctgacgc
     5581 aggatctggg tttgcgggtg atgaaacaat gtttcatgct attgaagatt ctatcaaaca
     5641 caacgttgat gttgtttcgg tatcatctgg ttttacagga acaggtcttg taggtgagaa
     5701 atattggcaa gctattcggg cattaagaaa agcaggcatt ccaatggttg tcgctacggg
     5761 taactatgcg acttctgctt caagttcttc atgggattta gtagcaaata atcatctgaa
     5821 aatgaccgac actggaaatg taacacgaac tgcagcacat gaggatgcga tagcggtcgc
     5881 ttctgctaaa aatcaaacag ttgagtttga taaagttaac ataggtggag aaagttttaa
     5941 atacagaaat ataggggcct ttttcgataa gaataaaatc acaacaaatg aagatggaac
     6001 aaaagctcct agtaaattaa aatttgtata tataggcaag gggcaagacc aagatttgat
     6061 aggtttggat cttaggggca aaattgcagt aatggataga atttatacaa aggatttaaa
     6121 aaatgctttt aaaaaagcta tggataaggg tgcacgcgcc attatggttg taaatactgt
     6181 aaattactac aatagagata attggacaga gcttccagct atgggatatg aagcggatga
     6241 aggtactaaa agtcaagtgt tttcaatttc aggagatgat ggtgtaaagc tatggaacat
     6301 gattaatcct aataaaaaaa ctgaagtcaa aagaaataat aaagaagatt ttaaagataa
     6361 attggagcaa tactatccaa ttgatatgga aagttttaat tccaacaaac cgaatgtagg
     6421 tgacgaaaaa gagattgact ttaagtttgc acctgacaca gacaaagaac tctataaaga
     6481 agatatcatc gttccagcag gatctacatc ttgggggcca agaatagatt tacttttaaa
     6541 acccgatgtt tcagcacctg gtaaaaatat taaatccacg cttaatgtta ttaatggcaa
     6601 atcaacttat ggctatatgt caggaactag tatggcgact ccaatcgtgg cagcttctac
     6661 tgttttgatt agaccgaaat taaaggaaat gcttgaaaga cctgtattga aaaatcttaa
     6721 gggagatgac aaaatagatc ttacaagtct tacaaaaatt gccctacaaa atactgcgcg
     6781 acctatgatg gatgcaactt cttggaaaga aaaaagtcaa tactttgcat cacctagaca
     6841 acagggagca ggcctaatta atgtggccaa tgctttgaga aatgaagttg tagcaacttt
     6901 caaaaacacg gattctaaag gtttggtaaa ctcatatggt tccatttctc ttaaagaaat
     6961 aaaaggtgat aaaaaatact ttacaatcaa gcttcacaat acatcaaaca gacctttgac
     7021 ttttaaagtt tcagcatcag cgataactac agattctcta actgacagat taaaacttga
     7081 tgaaacatat aaagatgaaa aatctccaga tggtaagcaa attgttccag aaattcaccc
     7141 agaaaaagtc aaaggagcaa atatcacatt tgagcatgat actttcacta taggcgcaaa
     7201 ttctagcttt gatttgaatg cggttataaa tgttggagag gccaaaaaca aaaataaatt
     7261 tgtagaatca tttattcatt ttgagtcagt ggaagaaatg gaagctctaa actccaacgg
     7321 gaagaaaata aacttccaac cttctttgtc gatgcctcta atgggatttg ctgggaattg
     7381 gaaccacgaa ccaatccttg ataaatgggc ttgggaagaa gggtcaagat caaaaacact
     7441 gggaggttat gatgatgatg gtaaaccgaa aattccagga accttaaata agggaattgg
     7501 tggagaacat ggtatagata aatttaatcc agcaggagtt atacaaaata gaaaagataa
     7561 aaatacaaca tccctggatc aaaatccaga attatttgct ttcaataacc aagggatcaa
     7621 cgctccatca tcaagtggtt ctaagattgc taacatttat cctttagatt caaatggaaa
     7681 tcctcaagat gctcaacttg aaagaggatt aacaccttct ccacttgtat taagaagtgc
     7741 agaagaagga ttgatttcaa tagtaaatac aaataaagag ggagaaaatc aaagagactt
     7801 aaaagtcatt tcgagagaac actttattag aggaatttta aattctaaaa gcaatgatgc
     7861 aaagggaatc aaatcatcta aactaaaagt ttggggtgac ttgaagtggg atggactcat
     7921 ctataatcct agaggtagag aagaaaatgc accagaaagt aaggataatc aagatcctgc
     7981 tactaagata agaggtcaat ttgaaccgat tgcagaaggt caatatttct ataaatttaa
     8041 atatagatta actaaagatt acccatggca ggtttcctat attcctgtaa aaattgataa
     8101 caccgcccct aagattgttt cggttgattt ttcaaatcct gaaaaaatta agttgattac
     8161 aaaggatact tatcataagg taaaagatca gtataagaat gaaacgctat tcgcgagaga
     8221 tcaaaaagaa catcctgaaa aatttgacga gattgcgaac gaagtttggt atgctggcgc
     8281 cgctcttgtt aatgaagatg gagaggttga aaaaaatctt gaagtaactt acgcaggtga
     8341 gggtcaagga agaaatagaa aacttgataa agacggaaat accatttatg aaattaaagg
     8401 tgcgggagat ttaaggggaa aaattattga agtcattgca ttagatggtt ctagcaattt
     8461 cacaaagatt catagaatta aatttgctaa tcaggctgat gaaaagggga tgatttccta
     8521 ttatctagta gatcctgatc aagattcatc taaatatcaa aagcttggcg agattgcaga
     8581 atctaaattt aaaaatttag gaaatggaaa agagggtagt ctaaaaaaag atacaactgg
     8641 ggtagaacat catcatcaag aaaatgaaga gtctattaaa gaaaaatcta gttttactat
     8701 tgatagaaat atttcaacaa ttagagactt tgaaaataaa gacttaaaga aactcattaa
     8761 aaagaaattt agagaagttg atgattttac aagtgaaact ggtaagagaa tagaggaata
     8821 cgattataaa tacgatgata agggaaatat tattgcttat gacgatggta gtgccttaca
     8881 atatgaaact gaaaaacttg acgaaataaa atcaaaaatt tatggcgttc taagcccatc
     8941 taaagatgga cactttgaaa ttcttggaaa gataagcaat gtttctaaaa atgccaaggt
     9001 atattatgga aatagctata aatcgataga aatcaaagcg actaagtatg actcacattc
     9061 aaaaacgatg acatttgatt tatacgctaa tattaatgat attgtggatg gattagcttt
     9121 tgctggagat atgcggtttt ttgttaaaga tgataatcga ataaaagctg aaactaaaat
     9181 tagaatgcct gaaaaaaata aggaaactaa agcagaatat ccctatgtat caagttatgg
     9241 gaatgtaatc gaattaggag aaggagatct ttcaaagaac aaaccagaca atttaactaa
     9301 aatggaatct ggtaaaatct attctgattc agaaaaacaa caatatctgt taaaggataa
     9361 catcattcta agaaaaggct atgcactaaa agtgactacc tataatcctg gaaaaacgga
     9421 tatgttagaa ggaaatggag tctatagcaa ggaagatata gcaaaaatac aaaaggccaa
     9481 tcctaatcta agagtccttt cagaaacaac aatttatgct gatagtagaa atgttgaaga
     9541 tggaagaagt acccaagctg tattaatgtc ggctttggac ggctttaaca ttataaggta
     9601 tcaagtgttt acatttaaaa tgaacgataa aggggaagct atcgataaag acggaaatct
     9661 tgtgacagat tcttctaaac ttgtattatt tggtaaggat gataaagaat acactggaga
     9721 ggataagtcc aatgtagaag ctataaaaga agatggctcc atgttattta ttgataccaa
     9781 accagtaaac ctttcaatgg ataagaacta ctttaatcca tctaaatcta ataaaattta
     9841 tgtacgaaat ccagaatttt atttaagagg taagatttct gataagggtg gttttaactg
     9901 ggaattgaga gttaatgaat cggttgtaga taattattta atctacggag atttacacat
     9961 tgataacact agagatttta atattaagct gaatgttaaa gacggtgaca tcatggactg
    10021 gggaatgaaa gactataaag caaacggatt tccagataag gtaacagata tggatggaaa
    10081 tgtttatctt caaactggct atagcgattt gaatgctaaa gcagttggag tccactatca
    10141 gtttttatat gataatgtta aacccgaagt aaacattgat cctaagggaa atactagtat
    10201 cgaatatgct gatggaaaat ctgtagtctt taacatcaat gataaaagaa ataatggatt
    10261 cgatggtgag attcaagaac aacatattta tgtaaatgga aaagaatata catcatttga
    10321 tgatattaaa caaataacag acaagacact aaacattaag attgttgtaa aagattttgc
    10381 aagaaataca accgtaaaag aattcatttt aaataaagat acgggagagg taagtgaatt
    10441 aaaacctcat agggtaactg tgaccattca aaatggaaaa gaaatgagtt caacgatagt
    10501 gtcggaagaa gattttattt tacctgttta taagggtgaa ttagaaaaag gataccaatt
    10561 tgatggttgg gaaatttctg gtttcgaagg taaaaaagac gctggctatg ttattaatct
    10621 atcaaaagat acctttataa aacctgtatt caagaaaata gaggagaaaa aggaggaaga
    10681 aaataaacct acttttgatg tatcgaaaaa gaaagataac ccacaagtaa accatagtca
    10741 attaaatgaa agtcacagaa aagaggattt acaaagagaa gatcattcac aaaaatctga
    10801 ttcaactaag gatgttacag ctacagttct tgataaaaac aatatcagta gtaaatcaac
    10861 tactaacaat cctaataagt tgccaaaaac tggaacagca agcggagccc agacactatt
    10921 agctgccgga ataatgttta tagtaggaat ttttcttgga ttgaagaaaa aaaatcaaga
    10981 ttaagacaaa agctatagaa aaaaatggtt tatgtactga gattagatag tgaggtgatg
    11041 acatagtttt gtgaaaacgg ccatttataa ctcaattatt tagtttactt cacttcacta
    11101 ttgatactat ttggagttat taatggactt agtttagata actaatgaat tgattgaaag
    11161 ggttagtatt gacaatattg gtcatattga ttagaaaata gagtctatca aaatttaaag
    11221 gctaatagag gtgatgagac aatttcaagg aattaagtag cgtttggttg cttaattcct
    11281 ttttatttgg aaggaagttt ttcgtaaccg cccaataacg aagtctattg gacggttacg
    11341 atatgatcac tgacttcgtc agtcttatct acaacctcaa aactgtgttt tgagcaacct
    11401 gcggctagct tcctagttta ctctttgatt ttcattgagt attagaacag aaaaaatgct
    11461 tggagtattt gtttgtgtgt ttatttttat ataacaaact ataaacaaaa caaaaatata
    11521 aaaaa
//
LOCUS       DAVKLW010000063        11365 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKLW010000063 DAVKLW010000000
VERSION     DAVKLW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.063"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(303..2192)
                     /locus_tag="VW324_001738"
     CDS             complement(303..2192)
                     /locus_tag="VW324_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9727317.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2330..3895
                     /locus_tag="VW324_001739"
     CDS             2330..3895
                     /locus_tag="VW324_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9727318.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3895..4635
                     /locus_tag="VW324_001740"
     CDS             3895..4635
                     /locus_tag="VW324_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9727319.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4744..4959)
                     /locus_tag="VW324_001741"
     CDS             complement(4744..4959)
                     /locus_tag="VW324_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727320.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(5843..7093)
                     /gene="ilvA"
                     /locus_tag="VW324_001742"
     CDS             complement(5843..7093)
                     /gene="ilvA"
                     /locus_tag="VW324_001742"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9727321.1"
                     /translation="MLSSKDIIKAHKILNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7143..7499)
                     /locus_tag="VW324_001743"
     CDS             complement(7143..7499)
                     /locus_tag="VW324_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9727322.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7737..7973)
                     /locus_tag="VW324_001744"
     CDS             complement(7737..7973)
                     /locus_tag="VW324_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727323.1"
                     /translation="MLADQNKADWSYDENTVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSSFERAGATKSARYIAKWILRNKKH"
     gene            complement(8080..9102)
                     /gene="ilvC"
                     /locus_tag="VW324_001745"
     CDS             complement(8080..9102)
                     /gene="ilvC"
                     /locus_tag="VW324_001745"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9727324.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVTEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9168..9644)
                     /gene="ilvN"
                     /locus_tag="VW324_001746"
     CDS             complement(9168..9644)
                     /gene="ilvN"
                     /locus_tag="VW324_001746"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9727325.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9637..11337)
                     /locus_tag="VW324_001747"
     CDS             complement(9637..11337)
                     /locus_tag="VW324_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9727326.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         3442 a         2582 c         1954 g         3387 t
ORIGIN      
        1 aaggaaaaca ttacaatgta gccatcatcc accttgcaaa aaaacttatc cgaaccctgt
       61 tttatatcct aaaaaagagc tgccatttga cgaacaaaaa gtgatataat ttctgaaatt
      121 aacacaggat tacctttgaa aaaatggact ttttcaatag ctttttgccg tgctctaaaa
      181 tcatcgccaa atcatcaaat tgaaataaga aatagtttag ataaaactat tgacttttct
      241 tatagtttgt cttttttcta tcttccatta tatcatactt tttcagtcta tggtagctct
      301 ctttaaaagg caccgatact gccgccacct ccgcctccag agaagccacc accagaactt
      361 ccacttccag aagatacaga gtaggtgctt gctgtatttg cgacactagc ataatggctc
      421 atttgtgctg ttgaatgata aaacatactg tgccagccat aagctacata gagattgata
      481 tctggatttt ccacttgaat ctgatgaacc ttcatcaaat gactaacctt gtccgcatag
      541 ccaaataagg tcgcatagac caagaggcga ttccagacca caatactttc cagttcagcc
      601 tgatccaatc gtgcaatctc acgcaacata ttttcaaaac tggtccagag atagtagacc
      661 tcagctcccg cttcatttag aacaccatca cgattatcta gtcgaagctt ccaataatag
      721 aaaacagaca aaactaaccc tagaaaacca agtattggca aagggaggta aagatagcca
      781 tgaacgtcta aactgtacaa gaacaaacca aatccgataa ataggggcag gatagtcaag
      841 gcacccatac ccacttgcaa ggccttttcc ccaccagtta aaggacgata ataatctggg
      901 agcccccaga aggaaactcg ttttctcact ccttcttgca tctggttcaa tacctcttca
      961 aaagaagatt tgagttgaag ccctcttgct tgaatccgtt tttcatcaga aactttggct
     1021 ctacgataaa gactatcaga taccttgtaa tccgcaaaca aattggaaag agtttcttct
     1081 tttttacctg aaaaagctag atttaggcag tctttctcaa agcttgacaa accatcttct
     1141 tttactagcc tcaaaccaac tgcatctcct tctgaaatga tagagacatt cccacggtct
     1201 atcacatcta gcaaggtagc ttgaataagt tgatcaaagg tgaattttcc agctcccttg
     1261 accaagggac tcacttcctc caaggaggtc gagtagactg cttctgataa aaccataggc
     1321 tctaattcca ttggtggttc atagagacga tgatttttgg catatttgac tgaaggagtg
     1381 gtctttcttc tataaataaa atagaagcag acactcaata acaaggagat agaaagtatc
     1441 gaagggagga cccaagtaac gagttgttta ctctgatctt tttctctaac aatcgagtct
     1501 tctatcttat taaactcttc taaacgattc cctttcaatc cctgatccct agcgctagca
     1561 aaatcggtcc gaggccaata ggcatgcaac tcaactccac gcttagccgg aagattgtct
     1621 aaacggatag tataatcaag gttactcttt tcaatcgttc cctctctaaa aagtttccct
     1681 gtatggaaaa agagtttttc agcccccttg tctcccctta catgaaattc aaacttttca
     1741 atagactctg aactatctgt cagaggttgc caatttaatt cagcgatatc atcataaagg
     1801 aaaagtaaat tttttaagtt ccagacgagg tcaacttcaa ctatgtcgcc ctcctgacct
     1861 ggattataga ctctcacagt ataaccatcc gcttcttctg ttacttcgct agtcacatct
     1921 gctagttctg caccgttttt cgcggcctga acctttggat gagggtcaat gtcaaacccg
     1981 ctaggcatct taccagcacg tccaagtccc acgatttgtc ccttaaagtc ctcctcaaac
     2041 tggtaaacta tcttctgtct aaactctgcc gtattatctg catgaatata caaatcccct
     2101 tgataagagt ttatcttgaa atcaatggca aaaacagaga gtggaagaag gcaaaacaag
     2161 cctaacacca gtaagaaaaa agtttttttc atcaactaag cccccttttt atctttctta
     2221 tcattatagc attttttcgt aagtaaaggc ttacttatgt tgaaaaatta cgcatttttc
     2281 gataaaatag gagacagtta ttttttaata gatcagaata ggagaaatca tgagaaaaat
     2341 atacttatct attttcacaa gtctcttgct gatgctagga cttgtcaatg ttgctcaagc
     2401 cgatgaatat ttacgcatcg ggatggaagc agcatatgct ccctttaact ggacccagga
     2461 tgatgatagc aacggagctg tcaaaatcga tgggaccaat cagtatgcca acggatacga
     2521 tgttcaaatc gccaagaaaa tcgctaagga cttaggtaaa gaacctttgg ttgttaaaac
     2581 caagtgggaa ggtctagtcc ctgcccttac ttctggtaag attgacatga ttatcgcagg
     2641 tatgagtcca actgcagaac gcaaacaaga aattgccttt tcgagcagtt actacactag
     2701 cgaaccagtt ttgcttgtca aaaaagattc tgcctacgca agtgctaaat ctttggatga
     2761 ctttaacggt gcaaaaatca cttctcaaca aggggtctac ctctacgact tgattgcaca
     2821 aatcccaggt gctaaaaaag aaacagccat gggagacttc gctcaaatgc gacaagctct
     2881 tgaggctggt gtcattgatg cttatgtttc tgaacgtcca gaagcactga ctgctgaagc
     2941 tgcgaactct aagttcaaga tgattcaagt agaacctggt ttcaaaactg gggaagaaga
     3001 tacagctatc gctatcgggc ttcgtaaaaa tgacaatcgt attagccaaa tcaatgccag
     3061 cattgaaacc atttcaaagg acgaccaagt tgccctgatg gatcgtatga tcaaggaaca
     3121 acctgccgaa gctacaacaa ctgaagagac tagcagtagt ttctttagcc aagtcgctaa
     3181 aattctttct gaaaactggc aacaactctt gcgtggtgct ggtatcactc ttttaatctc
     3241 tatcgtcgga accatcatag gtctcattat tggacttgcc attggtgtct tccgtactgc
     3301 tcctctctct gaaaacaaag ccatttatgg ccttcaaaaa ctagtcggct gggttctcaa
     3361 tgtctacatt gaaattttcc gtggtacgcc aatgattgtt caatcgatgg ttatctacta
     3421 tggaactgcc caagctttcg ggatcaacct tgaccgtaca ctggctgcta tcttcatcgt
     3481 ttcaatcaat accggtgcct acatgactga aatcgtccgt ggtggtatcc tagcagttga
     3541 caagggacaa tttgaagctg cgactgctct tggtatgacc cataaccaga ccatgcgtaa
     3601 gattgtccta cctcaggtag tccgtaacat cctacctgca actggtaatg aatttgtcat
     3661 caatatcaaa gatacatctg tattgaacgt tatctctgtt gtcgaacttt atttctcagg
     3721 aaataccgtg gcaacacaaa cctatcaata cttccagaca tttacaatca tcgccgtgat
     3781 ttactttgtc ctcaccttca ccgtaacacg tatcctacgc tttatcgagc gcagaatgga
     3841 catggatacc tacactacag gtgctaacca aatgcaaacg gaggatttga aataatgaca
     3901 caagcaatcc ttgaaattaa acacctcaaa aaatcctatg gacaaaacga agtgctaaaa
     3961 gacatttctc tcactgtcca caagggagaa gttatttcta tcatcggaag ctctggaagt
     4021 gggaaatcaa ccttcctacg ctccattaac ctacttgaaa cgccaactga tggacaaatc
     4081 ctttatcatg gacaaaacgt cctcgaaaaa ggctatgacc tcacgcaata ccgtgaaaag
     4141 ttagggatgg ttttccaatc ctttaacctc tttgaaaatc tcaacgttct tgaaaacaca
     4201 atcgtcgctc agacaactgt ccttaaacgc gaacgcacag aagctgaaga aattgccaaa
     4261 gaaaacctgg aaaaggtcgg catgggagaa cgctactggc aagctaaacc caaacaactc
     4321 tcaggtggtc aaaaacaacg tgtggccatt gctcgtgccc tttcaatgaa tcctgacgcc
     4381 attctctttg atgaaccaac atcagctctc gatccagaaa tggttggaga ggtcctcaaa
     4441 atcatgcaag atctggctca ggaaggcttg accatgattg tcgtaaccca tgaaatggaa
     4501 tttgcccgtg atgtctctca ccgtgttatc tttatggata agggcgtgat cgctgaagaa
     4561 ggtaaaccag aagacctctt caccaatcct aaagaagacc gaacaaaaga gttccttcaa
     4621 cgctatctca aataaaaaga aaaggctgca tcaaccgtgc agtctttttg ctgtcctcaa
     4681 aatgaaaagg cagattctct atctctacta cttgaaggtc aagaatccgc cgttatccaa
     4741 agattaatct tcaaattttt catgattttt ttcatagaaa tcaattaggc ctagggctgt
     4801 ttcaaatgaa atatttttta cttttgcacg accctgcgcc agagcaatga tagacatttc
     4861 acgcgcattc gtttctttag agatacggta gcctgtgatt ttcttatcac gaacccagcc
     4921 aacaactgat tctacttttt caaagtttga cttagccatg tttttctcct attttcttct
     4981 ttataatatt taattgtata cgtttttact acttttgtca ataaaaaaac atgtattcaa
     5041 cgaaaataat tagttcagaa ttttagcttc agaattttct tacttccttt ttgaatccga
     5101 taatatatag gttttactag tctctacacc ataaatattt gctaataaaa atactgtatt
     5161 attaccctct taaggtgcga aattattcaa gttcttaaaa aatgcttctc tatttccgta
     5221 agatagctta tttcttcgat ttaagaactg aagatgatag ttctaacctt tagaaatcca
     5281 agccttcact agctcaaaaa ctcctatctt tcttcctcaa taaatcatct gagagatagg
     5341 tattccttat atccttcaaa ttcatcactc ctttggatat tgaggtgcta gcctagaagc
     5401 agacacccga ttatctaaac catccttaat agagatgaca agacagataa gctcagttaa
     5461 ttacttatat atctttttaa tatggtatat ttgtcaacaa aaagaaatca aatcatacct
     5521 tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaagttgc tcaaaacact
     5581 gttttgaggt tgcagataga gctaacgtgg tttgaagaga ttttcgaaga gtattaaaga
     5641 cagtgtgact atgcttgtca atagatagga aatcaaaatg aaggatggaa atctgagcca
     5701 aacatcataa gtgctataat gaacagaagt aagaattgca cgataagttt cctaaaacca
     5761 tagaaatcgt cccctttctt catcttcatt atagcacata tacatcagtt gtacaaataa
     5821 tatgatattt ttttattagt cctcagacaa gcatattata aagcgtttca ttaccattta
     5881 agttaatata agctggatca aaaccttcca ttctacgaat caaacctgca taatcatgct
     5941 tatctgctaa agcgatccca attaatactg ggcctgttcc cttgctagct cgtttgatat
     6001 actcaaaacg tgtgatatca tcatttggcc ccaggatatc atttacaaac tcacgcaaag
     6061 ctcctggacg ttgtgggaaa ttgaccacaa agtaatgttt gataccatca taaatcaagg
     6121 cacgctcttc catttctggc atacggttga tatcattatt tcctccagaa atgatacaac
     6181 aaatggtttt ccccttaata tattcagcta aaacctctaa agaggcgata ctagccgctc
     6241 cagcaggttc tgcgactatc ccttgcttag agtaaaggtc aatcaaggtt tcagaaatca
     6301 atccctcatc gacacctacc aaagttttaa tatgttgacg agttgcttca taggtcaact
     6361 gacctacctt ttgcacagca atcccatccg caaatttatc aatttccttg agtttgactg
     6421 gacctccagc ctcaaaggca gctttcatgg aacgcgctcc attcgcctct actccgataa
     6481 cctcaatctc tggacttgtt tccttgatat aggtagaaac cccggcaatg agaccgccac
     6541 caccaacagg aaccaagaca gcatcaaaat caatcgattc ttttcgagct tcttctaaaa
     6601 tctcataagc aactgttcct tgacctgctt gaacatgagc atcatcaaaa ggatcaataa
     6661 aggtacgatt ttcagagact gtaaattctt gagctgcttt ggctgaggca tcaaaggtat
     6721 ctccaactag tttaatagtt acaaaatccc caccaaaaaa gcgaacctga ccaatctttt
     6781 gttgtggcgt agtaataggc ataaagatag tagcaggaat tttcatttca ttacaagtat
     6841 aggctactcc ctgcgcatga tttcccgcag aagcgcagac taccccacgt tcacgttctt
     6901 ccttgctgag ctgggaaatg gcataatagg caccacgaat tttaaaggag cgaacacgct
     6961 gggcattttc ttttttcaaa taaatcttag caccatactt ctccgataaa taatgatcgt
     7021 aatccagtgg agtattcaca accacaccgt tcaagatctt gtgagccttg attatatctt
     7081 ttgaacttaa catataaaac ctctttttct ataaaaaaca gtcactaatg actgtttttt
     7141 agttattcat ctccgaaaac ttcagtaaca ctttcatggt atttatgagc catacgaatc
     7201 gaaaactctt taggaagctc ataacgattt gtggaaatcc aatcatataa ccattctacc
     7261 aaactttctt cactatcatt acgatggaag ataaaatagt ttaaaatttc tttatattct
     7321 acatcattca aatccaacgt gtctacaaat tgactacgag gaacaaaatt agaatctaat
     7381 tttcttagtt gatctgtcaa ttctcccaat tgtaaatcca actgaaaaaa tagtttttgc
     7441 agttgcctat cagtttcatc acatcctgtc ttgatagaaa acaaattggt aatattcata
     7501 tgatttcaac ccctaaataa gtacatctaa tatcccacga atagctgaag ctaaagtttt
     7561 cgcagtccag tcatttaaac ccgattcttt caatccagat gcaataattt gctctatttg
     7621 atataaaccg ccatctatcg tctcatttat tctgcgaaga ggaccttcaa tataggataa
     7681 agcttctccg aatctaacag ctgatttagg tcctaatgat ttaccaacta tatttgttaa
     7741 tgttttttat ttctcaaaat ccatttcgct atataacgag cagatttagt cgcaccagct
     7801 ctctcaaacg aagaatgaat ttcaagacct tcttctctag ctatttgtgc caatttgaag
     7861 aattgttcaa aggtcatatg caacctacca tcttcaaaat tagcatcatc ataaatgtta
     7921 attacagtat tttcatcata cgaccaatct gctttatttt ggtctgctag cactattacc
     7981 gacggtgtaa ttacattata caactagaac caataataac gatgcaattt ttctcataat
     8041 ctttttcata aaatcatctc cagcgctata tatttctaat tagttataga ttttgaatgc
     8101 atcatcgtcg tttttaccaa cgaatggcat tgctttacgc aattctgcac caactttttc
     8161 aatttcaagg ttagctgctt gttcacggta agcagtcaat tttggacgtc cagctttata
     8221 gtcatttaca aagtcatttg caaatttacc attttggatg tctgccaaga cagccttcat
     8281 attttcttta acttgttcag tgattacacg tggacctgat acatagtcac cgtattcagc
     8341 agtgtttgaa atagattgac gcattttctt gaatccacct tcgtagatca agtcaacgat
     8401 caatttcatt tcgtgaagaa cttcaaagta agccaattct ggagcgtaac ctgcttctgt
     8461 caagacttcg aaacctgctt cgataagggc agtcaaacca ccacaaagta cagcttgttc
     8521 accaaacaaa tcttcttcag tttcttcttt gtaagttgtt tcaagaagac ctacacgagc
     8581 cgctccaaca cctttacacc agtccatagc aatgttttta gcatttcctg ttgcatcttg
     8641 gtatactgca taaagagctg gaacaccaaa tccttcttcg taagtacgac gtaccaagtg
     8701 tcctggtcct ttaggagcac acatgaagac atctacatcc gcaggaactt tgataaattc
     8761 aaagtggatg ttgaaaccat gggcaaatcc aactgcgttt ccagcttcca agtttggagc
     8821 gatttctgct tcgtacaatt cttgttgaat ttcgtctggc gccaagatca tgataacatc
     8881 agccaactta gtagcttctg ttactgtgta agtatcaaat ccatcttctt ttgctttatc
     8941 aaaagattta cctggacgta caccgataat aacgtcacga cctgaatcac gcaagttttg
     9001 agcatgcgca tgcccttgtg aaccataacc gataacggcg atttttttac cgtcaagtgc
     9061 tgctacttta acatcttttt catattccat ttgaactgtc atagcttttc tctcttttct
     9121 attatttatt gccttttaag ctggtttaac aaatttaagt tggattttta atcgcgggta
     9181 aatccagttg cacccgttcg agcaatattg cgaataccgt atgggcgaat gactcgcaat
     9241 agggcttcgc tcttttctgc atttcccgtc atctgaatgg taatcgagct tggcgctacg
     9301 tctaccactg ttgcacggaa aggttgaata atcgctaaaa tctcagctct cttctcagct
     9361 ggcgctgaca tcttaaccaa aatcacctcg cgctccaaat gaggcttgtc tgtaatatct
     9421 cgaatgcgaa tcacatcaat ctgacgattg agctgtttga tgatttgctc cacttcatca
     9481 tgagaagcaa catcaataat aatagtgata cgcgatacat tcggatcttc tgttgctcca
     9541 acagagatgc tttcaatatt aacctgacga cgagatagga cacctgtaaa gcgattgagg
     9601 actcctgaac gattttgtag ttttgctgtt aacattctac gcatggaact gcacccccaa
     9661 catctcatga ttactcttac cagccggtac cattggtaac acctgttcct tacgagaaat
     9721 atctacctca attagcatag gaacatcctc agtgatgact tcaaggtctt gagccaaggt
     9781 ctcaggattg tcaaacttat agtttttaat accataagcc tgcgccatca attggaaatc
     9841 aggaagggta tcaaagaccg actctgatgt tctgccttca tagaaggatt cctgccactg
     9901 gcgaaccatt ccgagtgaat gattgttcag cataaccacc ttgattggca ccttgtaaat
     9961 attcaaaata gccaactcct ggttggtcat ttggaaacca ccatccccaa caaacaagac
    10021 tacttcctta tctgggttag caattttagc accgattgct gctggaattc caaagcccat
    10081 tgttcccaaa ccacctgaag tcactaactg acgttcattt tggtagggat aatactgagc
    10141 tgtccacatt tggtgttgac caacgtctgt taccacaatg gcatctccat tcgtcaattc
    10201 accaattcgt tcaataactg cttgcggttg aaccacacgc tctttcttgt catacgaacg
    10261 aacacgattc ttgtctttag tgactttctc aatccacttt tcagtattgt tgtgaactgt
    10321 tggttctgct agcaacattt gcaaggcctt cttagcatct ccaactacag gaatgtctgc
    10381 actgataatc ttgccaatct cagctgggtc aatatcaatg tgggcaacct tagcattctt
    10441 agcgaaagtc ttaggattcc ccgtcaaacg gtcatcgaaa cgagaaccaa tactaatcat
    10501 aaagtccgct tccgtcatgg caatatttgc tgcgaatgac ccgtgcatgc ctcccattcc
    10561 aagaaagagt gggtgactcg ttgcaatcgt tccttgtccc aaaagactgg ttaccactgg
    10621 aatttgatag cgttctgcaa attcatttag ttccgtagca gcctcagcat aactaattcc
    10681 accaccagct aacaagactg gctttttagc cttggacaat tgcttcaaga ttttcttgat
    10741 ttgcatatca ttcggctcaa gagtcggctg ataacttggt aaattcactt ctggtgaata
    10801 aatgaagtct gtttctaaag cagatatgtc ttttggtagg tcaattacaa ctggccctgg
    10861 acggcctgta gttgcgatat ggacagcttc cgtaatgata cgcggaatat cagctgtctc
    10921 acgaacttgg taattgtact tagtgattgg catggtaatt cctacgatgt ctgcctcctg
    10981 aaaggcatcc ttcccaatcc ctgctcgcgc cacctgacct gtaaagacca aaaggggaac
    11041 gctatcgctc atagcatccg caatccctgt aatggcattt gttgctcctg gtccactagt
    11101 gacgacggca acacccaact ttccagttga tttggcatag ccttcagctt catgcaaaca
    11161 accttgctca tggcgcccta gaatgtggcg aatgccttta aaattatata tcgcatcata
    11221 aaaaggcaaa accgcaccac caggataacc aaagatggta tcaactccta aatcacgaag
    11281 tgtttccaaa actaggtccg accccgtctt aggagattct aaactgattt tctccattat
    11341 tcccctttct ttcctcttaa aaata
//
LOCUS       DAVKLW010000064        11010 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKLW010000064 DAVKLW010000000
VERSION     DAVKLW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.064"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..2294
                     /locus_tag="VW324_001748"
     CDS             <1..2294
                     /locus_tag="VW324_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9727327.1"
                     /translation="QTDFSNVAEYRIGGTNLIYTPNTLLRNYQNILDEVLPALNSVEY
                     KSEAIRKVLDVSKDVSLTELYLEEQFNTTKTNLKDSLTKLLTADAAIAENNNKVIDNY
                     VIEKIKNNKEALLLGLTYLERWYDFKYRDTKAKDLVMYHLDFFGKSNSSALDNVIELG
                     KSGYNNLLAKNNVITYNVLLAKNYKTNNLFDALEKYRKAFVPDKTNNEWFKEQTKAYI
                     VEEKSTIKEVSDKQSIAGSPYSIGVYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANIS
                     TIGFGAYDRYRSKEHPAGTDLNDYVEKKAKEAAVRFRDHYDYWYRILDDKNKEKLYRS
                     VLVYDAFRFGTDEKEDKDTYQATFETNHPAIKHFFGPAGNNVVHNSNGAYATGDAFYY
                     MAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRNGLGPEFYAKGLLQAPDHPNDPTV
                     TINSILKYDQSEESTRLQVADPTQRFGSVDDLNKYMHNMFDVIYMLEILEGKAVAKLD
                     TNQKYDLLRKIENEYKPDPDGNSVYATNVVRRLKPEELTKLTTFNSLIEHDIITRRGY
                     VDEATYKRNGYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKGYKDGMVPYIS
                     NQYEKEAKAQGKVITSYGKQIGLVTDEIVLSKVFNNQYNSWIDFKKDMYKEREDKFGK
                     LNKVSFIDPNGSWARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRVLKLK
                     KAIFKAYLDQTGDFRSSIFENKK"
     gene            2338..7551
                     /locus_tag="VW324_001749"
     CDS             2338..7551
                     /locus_tag="VW324_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9727328.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            7805..8656
                     /gene="ylqF"
                     /locus_tag="VW324_001750"
     CDS             7805..8656
                     /gene="ylqF"
                     /locus_tag="VW324_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9727329.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            8643..9422
                     /locus_tag="VW324_001751"
     CDS             8643..9422
                     /locus_tag="VW324_001751"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9727330.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            9438..>11010
                     /locus_tag="VW324_001752"
     CDS             9438..>11010
                     /locus_tag="VW324_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9727331.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLLYYDHLHNL"
BASE COUNT         3803 a         1790 c         2226 g         3191 t
ORIGIN      
        1 atcaaactga tttttcaaat gtagcagagt atagaatagg cggtacaaac ttaatttata
       61 caccgaatac attacttcgc aattatcaaa atattttaga tgaagtatta ccagcattaa
      121 atagtgtaga atataaatca gaggcaatta gaaaagtatt agatgtttct aaggatgtta
      181 gtttaacgga gttatattta gaagaacaat tcaataccac aaaaactaat ttgaaagata
      241 gtttgacaaa acttcttact gcagatgctg caatagctga aaataacaat aaggtaatag
      301 ataattatgt aatcgagaaa attaaaaata ataaagaagc tttacttcta ggattaacat
      361 acttagagcg ttggtacgac ttcaaatatc gtgatacaaa agcgaaagat ttagtcatgt
      421 accacttgga tttcttcggt aaatcaaata gttcagcgtt agataatgtt atcgaactag
      481 gtaaatcggg atataataac ttactagcaa aaaataatgt tataacttat aatgttttat
      541 tagcgaaaaa ttataagact aataatttat ttgatgcatt agagaaatat agaaaagcat
      601 ttgtaccaga taaaacaaat aatgagtggt ttaaagaaca aactaaagca tatattgtag
      661 aagaaaaatc tactattaaa gaggtaagtg ataagcaatc aatagctggt agtccatatt
      721 caattggagt atatgataga ttaactagcc catcttggaa atatccaagt atggtgttgc
      781 cgctattaac attacctgaa aaatcggtgt ttattatagc caacatttcg actataggtt
      841 tcggtgctta tgatagatat agaagtaaag aacatccagc aggaactgat ttaaatgact
      901 atgttgagaa aaaagctaaa gaagcggctg ttagatttag agatcactat gattattggt
      961 atagaatact agatgataaa aataaagaaa aattatatag aagtgttcta gtctatgatg
     1021 catttagatt tggtacagat gaaaaagaag ataaagatac atatcaagca acttttgaaa
     1081 ctaatcatcc agctataaaa catttcttcg gtccagcggg aaataatgtt gttcataatt
     1141 ctaatggagc atatgctaca ggggatgcat tctattatat ggcatatcgc atgcttgata
     1201 aagatggtgc ggtaacttat acacacgaaa tgacacataa ctcagataga gaaatttatc
     1261 ttggaggcta tggaagacga aatggacttg gaccagagtt ttatgctaaa ggactgctac
     1321 aagctcctga tcatcctaat gatccaacag tcactattaa ttccatatta aaatatgatc
     1381 aatcagaaga atcaacaagg cttcaagtag cagatccaac tcaacgattt ggaagtgttg
     1441 atgatctaaa taagtatatg cataatatgt ttgatgtaat atacatgctt gaaatattag
     1501 aaggtaaagc tgtagctaaa ttagatacaa atcaaaaata tgatttatta agaaaaatag
     1561 aaaatgaata taaacctgat cctgatggaa attctgttta tgcaacgaat gttgtacgta
     1621 gattaaaacc agaagaactt actaaattaa ctacatttaa cagtttgata gaacatgata
     1681 ttataacaag aagaggttat gtagatgaag caacttataa gcggaatggc tactatacaa
     1741 ttaatctatt ctcaccaatt tattcagcat tgagcagtaa aataggaaca ccaggagatt
     1801 taatgggacg acgtattgca tttgagttat tggcagccaa ggggtacaaa gacggtatgg
     1861 ttccatatat ttctaatcag tatgaaaaag aagcgaaagc tcaaggaaaa gttattacat
     1921 catatggtaa acaaataggt ttagttactg atgaaattgt tctttcaaaa gtatttaata
     1981 accagtataa ttcttggata gatttcaaga aagatatgta taaagaaaga gaagataaat
     2041 ttggaaagtt aaataaagtt agttttatcg atccaaacgg ttcttgggct cgacaacaga
     2101 aagtgactat tgataatatt aatagacttg aaaaaatgat tgaagatgca gtaaaatttg
     2161 atgctgagga tgaagttgcc aaattatatc ctgagacaaa tagtagagtt cttaaactga
     2221 aaaaagcaat ctttaaagct tatcttgacc aaaccggtga ttttagaagt tcaatttttg
     2281 agaataaaaa atagtgttta ctattaggaa ataaaaagtg aaggtggagg attagaattg
     2341 tcattattaa aaaaagataa attttccatt cggaaaataa agggtattgt tggttcggta
     2401 ttcttgggga gtcttttatt tgcaccgtca gttgttggtg catcgactta tcattactta
     2461 gattatagta gtttgacaca aactgaacgt gatcaactca aacaaggtag acctgatgaa
     2521 tccaaagagt catatgcttt ggattatgaa aaagacgcac taccaaacac aggaagttct
     2581 caatctatta tgactgcatt gggtttgtta gctatcggga gtcttattgt gattatcaca
     2641 aaagataata gaaataaaaa aatagctaca tttttgattg taggggcgac aggtttagtt
     2701 acactatcaa ctgcttcagc acttaatttg aatgctaaca tccatgagtc tggacgtgat
     2761 ggtgtgttgc aaatttctgg ttacagatat gttggttact tggagcttga tgataaaact
     2821 gtttcgtcgg tttctccagc atcaactgtt tcgccagttg agcaacccaa agttgtaaca
     2881 gagaaaggcg aaccggaagt tcatgagaag cctgactata cgcaaccaat aggtgcaaac
     2941 cttgttgagc ctgaagttca tgagaaacta gcatacactg aaccagtagg gacaacagga
     3001 gtagatgaaa atggaaactt gattgaacca cctgttaacg acatcccaga atatactgag
     3061 ccagtaggta caacaggagt ggatgaaaat ggaaacttga ttgagccacc tgttagcgac
     3121 attccagagt atactgaacc aatatctaca gtttcagaag ttgcatcaga aagagaagag
     3181 ttgccttctt tacataccga tattcggaca gagactattc ctaaaaccac tatagaagaa
     3241 tctgatccta gcaaatttat aggcgatgat tctgttagac aagttggtga ggatggcgaa
     3301 cgtcaaattg ttactagcta tgaagagtta catggcaaaa aaatcagtga cccagttgaa
     3361 acagtaacta ttttgaaaga aatgaagcct aaaattcttg taaaaggtac caaagaaaaa
     3421 cctaaagaaa aaacggctcc tgttttgacc ttagatagaa caaatacaaa tgtattaaac
     3481 cgatccgcaa ctctttctta tcatttagtt aatacagatg gagtcacaat caataaaatt
     3541 actgcgacaa ttaaagatgg aaatgaaatt gtcaaaacag tagatttgac ttcagagcaa
     3601 ttagataagc aggtagaaga tttaaaattc tacaaggact ataagataga gaccactatg
     3661 acctatgacc gtggcaaggg agaagaaact gcaacattag aagagaaacc acttcgcttg
     3721 gatttgaaaa aagttgaaat taaaaatatc gcatctacta atttagtgaa ggtaaatgac
     3781 gatggtactg aaacacctag tgattttatg actgaaaaac cttcagatga agatgtgaag
     3841 aaaatgtact taaaaattac tagtcgtgat aataaagtaa cacgtttagc agttgataaa
     3901 attgaagaag tgactgaaga aggtaagaaa ctttataaaa tcactgccga agctcaagat
     3961 ttgattcaac atacagaccc aacgaaggtt cgtaacaagt atgttcatta tattgaaaaa
     4021 ccagttccaa aagttgatga cgtttattat aactttaaag agttagtaga tgccatgaat
     4081 gctgataaaa atggtacatt taaaatcgga gcagatttga atgcaacgaa tgtaccaaca
     4141 cctaacaaac aatatgttcc aggtacgttt aagggacatc tatcaagtgt tgatggtaaa
     4201 caatatacaa ttcataacat tgcccgtccc ttatttgacc gtgttgaaaa tggttctgtg
     4261 aaaaatatta atttaggtaa tgtggatatc aatatgcctt gggctgacgg aatagcacct
     4321 gttgccaata tggtgaaaaa tgcaactgtt gaagatgtta aggtaactgg taatgtagtt
     4381 gctaataaca atattgcggg tatcgtcaat aaaatcgata gtggtggtca gttgacaaat
     4441 gtagccttca tcggtaatct tacaggtgtt ggtgataaag gtcaatatat ggctggcatt
     4501 gccggtgaga tttggagagg taatcttgca aaagcatatg tcgaggctga tattgttgct
     4561 aacagagccc gtataggtgg tttagttgct aaaacagata atggtaacga ctctatggga
     4621 attggtaaat acggttcaat tcgtaaatca gtcaccaaag gaaccattaa gactaaagtt
     4681 ttgtttgaaa caggtggatt tatcaatagc aatcttcctt ttggtaaact tgaagataat
     4741 atttcaatga tgagagttga aaatggtgaa gagttctttg gttcttccga tttggactac
     4801 gatggtggtt atttcacaaa tggctggtta gagcgtaact ttgttgtcaa aggtgtcagt
     4861 tcaggtaagc attcatacaa acgttctcgt gacaaaatta aagaaattag tcaagatgaa
     4921 gctaataaga gaattgcaaa ttttggactt actgctgata aatacgaaat caacgaacct
     4981 gtggtcaatc gcttaaatcg tttaaccaga agagaagatg aatataagtc aactcaagac
     5041 tacaaatccg aacgtgattt agcttatcgt aatattgaga aacttcaacc attttataat
     5101 aaagaatgga ttgtcaacca aggtaacaaa cttgcagaag attcaaactt agctaagaaa
     5161 gaagtccttt ctgttacggg gatgaaggat ggtcaattcg tgaccgattt atcagatatt
     5221 gataagatta tggttcacta tgctgatggt actaaggaag aaatggatgt tactaagaat
     5281 actgattcta aagtccaaca agtgcgtgaa tatagtgtat ctggtcttgg tgatgttgtt
     5341 tatacaccga atatggttgt taaaaatcgt gataaattga ttgctgatgt taaatctcaa
     5401 ttatcaagtg tggaattgat ttcacaagaa gtaagagatt taatgagtag acgtgataaa
     5461 cctgcagaga atacggatga acgtaagaat ggttatatta aagatctata cttagaagaa
     5521 agctttgccg aagtgaagca aaatctagat aagttagtca aatccttggt cgaaaatgaa
     5581 gaccatcaat tgaatggtga tgaagctgcg ataaaatcac ttctcaagaa agtggaaacc
     5641 aataaagcta aaatcatgat ggctctgact tatctcaatc gttactatga tatcaaatac
     5701 ggtgacataa gtataaaaaa catcatgatg ttcaaacctg atttttatgg taaaacacca
     5761 agtgttattg acagactcat caacatcggt tcaagtgaaa agaacttgaa aggggatcgg
     5821 actcaggatg cttaccgtga aatcatcgca ggtaatactg gtaaaagtaa tttgcgtaac
     5881 ttcttagaat acaacatgcg tctgtttaca gaggataaag acatcaatga ttggtttatc
     5941 cactcagcta agaatgttta tgtttcagaa cctaagacaa caaatacaga gttgaaagat
     6001 aaacgtcatc gtgtgttcga tggtttagat aacggtgttc atggtcgtat gattttacca
     6061 cttctaacgc ttaaagatgc ccatatgttc ttaatttcaa catataacac gatggcgtac
     6121 agttcatttg agaaatatgg aaaacataca gaagaagctc gtaatgagtt caaaactaag
     6181 attgatgaag ttgcacatgc tcaacaaact tatttagatt tctggtccag attagctctg
     6241 cctaatgttc gtgatagact tcttaagagt caaaatatgg taccgacacc agtttgggat
     6301 aaccagacct acaatggttc tcctgtaggt cgtcgaggat ttgacagtaa aggtaaccct
     6361 atagctccaa ttcgtgaatt atatggtcca acatggcgac accatgatag agactggcgt
     6421 atgggcgcta tggcatcaat attccctaat ccaaataatg atgacaaggt cttgtttatg
     6481 gtgacggata tgattagccc atttggtatt tcagccttta ctcacgaaac aacacacgta
     6541 aacgatagaa tgctttactt cggtggtcat aagcatcgtc aaggtacaga cgttgaagcc
     6601 tatgctcaag gtatgttgca aacacctgac agctcaacga ctaatggtga atacggcgca
     6661 cttggaatta acatggctta ccatcgtcca aatgatggta accaatggta caacccagat
     6721 cctgacaaat taaagacacg tgatgacatt gaccgctaca tgagaaacta caatgaagct
     6781 atgatgttgc ttgatcatgt agaagctgat gctgtacttc ctaaaattaa aggagacaat
     6841 agtaagtggt tcaagaagat tgacaaagaa atgcgctcta agattcagta taatgatctt
     6901 ttaggtccaa accaatggga tagtatccgt gatttgaaag atgaagaaaa ggtaatgact
     6961 ttatctagcg tcaatgattt agtggataat aacttcatga caaaacatgg aaatccaggt
     7021 aatggacgtt atagaccaga agattttaca ccaaactcag cttacgtaaa tgtcaatatg
     7081 atggcaggaa tttacggtgg gaatacgagt caaggtgctc ctggttcatt atcatttaag
     7141 cacaatgctt tccgtatgtg gggctattat ggttatgaaa atggctttat tagttatgta
     7201 tcaaataaat acaaagcaga agcagataaa aacaaccacg gtttactaag cgataagcta
     7261 ataattaata aagtatcgaa aggaaacttt aatactcttg aagagtggaa gagacattgg
     7321 tacggggaag ttcttgctaa agctaagaaa ggttttgagg ctatcgatat tgatggcgtc
     7381 catatcagta actatgatga gttaaggcct ttatttgata aagcagttga agaagatttg
     7441 aagaaaccag atgacttctc tcacactgtt gctcttaaat ctaaggtctt caaagccctt
     7501 cttaaaaaca cagacggttt ctttaataaa ctgtttaaag aagacattta aatgtttaga
     7561 atatagaagg aacagttttt tgctccctct gaaaagtcat catttgatgg cttttttcta
     7621 tatagggtac ttgaaaggct atcatcggac aaaataaaga aggcatgata taatataagg
     7681 tagatttcta tgtcataaaa caaaaaactg tttgaacatc attcatttga gaacccttta
     7741 tgttcaaacg atagtaaaat aaaataggat ctctaaaccc ttgctacgaa aggaaaaaaa
     7801 ctcaatggct actattcaat ggtttcctgg tcacatgtct aaagcgcgtc gacaggtgca
     7861 ggaaaattta aaatttgttg attttgtaac gattttggtg gatgcacgct tacctctatc
     7921 tagtcaaaat cctatgttga ccaagattgt tggtgataaa ccaaaactct tgattttaaa
     7981 taaggcggat ttggctgatc cagcaatgac caaggaatgg cgtcagtatt ttgaatcaca
     8041 aggaatccaa actctggcta tcaactccaa ggagcaagtg actgtaaaag ttgtaacaga
     8101 tgcggccaag aagctcatgg ctgataagat tgctcgccag aaagaacgtg ggattcagat
     8161 tgaaaccttg cgtactatga ttatcgggat tccaaacgct ggtaaatcaa ctctgatgaa
     8221 ccgtttggct ggtaaaaaga ttgctgttgt tggaaacaag ccaggggtca caaaaggtca
     8281 acaatggctt aaaaccaata aagacctgga aatcttggat acaccgggga ttctctggcc
     8341 taagtttgag gatgaaactg ttgcacttaa gttggcattg actggagcta tcaaggatca
     8401 gttgcttcct atggatgagg ttaccatttt tggtatcaat tatttcaaag aacattatcc
     8461 agaaaagctg gctgaacgct tcaaacaaat gaaaattgaa gaagaagcgc ctgtgattat
     8521 tatggatatg acccgcgccc tcggtttccg tgatgactat gaccgttttt acagtctctt
     8581 cgtgaaggaa gtccgtgatg gcaaactcgg taactatacc ttagatacat tggaagacct
     8641 cgatggcaac gattaaagaa atcaaagaat tccttgtgac agtcaaggag ttagaaagcc
     8701 ctattttttt agagcttgaa aaggataatc gctcaggagt tcaaaaggaa atcagcaagc
     8761 gtaaaagagc cattcaagct gaattagatg aaaatttgcg cttggaatcc atgctttctt
     8821 atgaaaaaga actttataag caaggattga ccttaattgt aggtattgat gaggttggtc
     8881 gtggtcctct tgctggtcct gtagtcgctg cggccgtcat tttacctaaa aattgtaaga
     8941 ttaaaggtct caacgacagc aagaaaattc ctaaaaagaa acatctggag attttccaag
     9001 ccgttcaaga ccaagccttg tcgattggaa ttggtatcat agataatcag gtcatcgacc
     9061 aagtcaacat ctatgaagca accaaactag ccatgcaaga agcaatctcc cagctcagcc
     9121 ctcagccaga gcaccttttg attgatgcca tgaaactgga cttgcccatt tcacaaacct
     9181 ccattatcaa aggagatgcc aactccctct ctatcgcagc agcatctata gtagccaagg
     9241 taacacgtga tgaattgatg aaggaatacg atcagcagtt ccctggctat gatttcgcta
     9301 ctaatgcagg atatggcaca gctaaacatc tggaaggcct cacaaaacta ggagttaccc
     9361 caattcaccg aaccagcttt gaacccgtta aatcactggt tttaggtaaa aaagaaagtt
     9421 aattgaaagg aaataccatg gaggaacagt cggaaatagt ccgttctaag aaagaattcg
     9481 cctttgcatc cagcactata ctatcccaag ttggtcgagg aatcattgtc ggcctcatcg
     9541 ttggaattat cgtcggatcc tttcgtttct taattgaaaa gggcttccac ctgatacaag
     9601 gagtttatca agatcaaggg tacttagtgc gcaatctttt tgtactggtt ttgttttata
     9661 tactcatctg ttggctcagt gccaaactaa cacggtcaga aaaagatatt aaaggctcag
     9721 gaattcctca agtcgaagcc gaactgaaag gcctcatgtc cctcaactgg tggggcattc
     9781 tttggaaaaa atatgtgcta ggtattcttg ctattgccag tggactcatg ctgggtcgag
     9841 agggacccag tattcaactt ggagcagttg gtggtaaagg aattgccaag tggctcaaat
     9901 ccagtccagt agaggaacgt tccttgattg ccagtggagc tgcagcaggt ttagccgcag
     9961 cctttaatgc tcctattgca gcacttctct ttgttgtaga agaagtctat caccattttt
    10021 cgcgcttttt ctgggtctca actctagcag ccagcatcgt agcaaacttt gtgtctctac
    10081 tcatgttcgg tttgacacca gtattggata tgccagataa cattcctccc atgaccctag
    10141 atcagtattg gatatatctc gtcatgggaa ttttccttgg attttcaggt tttctctatg
    10201 agaaagctgt attaaacgtt ggaagagttt atgacttgat tggtcaaaaa atccatttgg
    10261 atagggctta ttatcccatc ttggctttta tccttatcat accagtcgga atcttcttac
    10321 ctcaaatcat tggtggcgga aatcagcttg tcctttcttt aactgaacaa aattttagtt
    10381 tccaagtttt attagcttac tttttaatcc gctttatttg gagtatgatt agctatggaa
    10441 gtggactgcc aggaggaatt ttcctcccca ttttagctct tggttctttg cttggtgcct
    10501 tagttggtgt tatctgtgtc aatcttggac ttgtcagtca agagcaattc cctatatttg
    10561 tcattctagg aatgagtggc tattttggag ccatatcaaa agctccctta accgctatga
    10621 tcctcgtaac tgagatggta ggagatattc gcaaccttat gccacttggt cttgtcactc
    10681 ttgtttctta tattatcatg gatttgctca aaggtacgcc agtctatgaa gccatgctgg
    10741 aaaaaatgct tccagaagaa gtatctagcg aaggagaagt tacacttatc gaaataccag
    10801 tttctgataa aattgctggg aaacaagttc atgaactcaa cttaccacac aacgtcctca
    10861 tcacaactca agtccataat ggcaagagcc aaacggttaa cggctcaacc agaatgtatc
    10921 tgggtgatat gattcacctg gttattccaa aaagtgaaat tggaaaagtc aaagatttgt
    10981 tgttgtatta tgatcattta cataatttat
//
LOCUS       DAVKLW010000065        10839 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKLW010000065 DAVKLW010000000
VERSION     DAVKLW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.065"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            195..692
                     /locus_tag="VW324_001753"
     CDS             195..692
                     /locus_tag="VW324_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9727332.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            692..2347
                     /gene="dnaX"
                     /locus_tag="VW324_001754"
     CDS             692..2347
                     /gene="dnaX"
                     /locus_tag="VW324_001754"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9727333.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2375..2569
                     /locus_tag="VW324_001755"
     CDS             2375..2569
                     /locus_tag="VW324_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9727334.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2719..3489
                     /gene="sufC"
                     /locus_tag="VW324_001756"
     CDS             2719..3489
                     /gene="sufC"
                     /locus_tag="VW324_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9727335.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3517..4779
                     /gene="sufD"
                     /locus_tag="VW324_001757"
     CDS             3517..4779
                     /gene="sufD"
                     /locus_tag="VW324_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9727336.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4790..6016
                     /locus_tag="VW324_001758"
     CDS             4790..6016
                     /locus_tag="VW324_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9727337.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPLKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6003..6443
                     /locus_tag="VW324_001759"
     CDS             6003..6443
                     /locus_tag="VW324_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9727338.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6497..7909
                     /gene="sufB"
                     /locus_tag="VW324_001760"
     CDS             6497..7909
                     /gene="sufB"
                     /locus_tag="VW324_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9727339.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFGDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8140..9381)
                     /locus_tag="VW324_001761"
     CDS             complement(8140..9381)
                     /locus_tag="VW324_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9727340.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNTDHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9484..10593
                     /locus_tag="VW324_001762"
     CDS             9484..10593
                     /locus_tag="VW324_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9727341.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3215 a         1977 c         2347 g         3300 t
ORIGIN      
        1 atatgattct ttctaatgag ttgttttgtt gcttttcatt ataggtcata tgggactttt
       61 tttctacaca aaataggctc cataatatcc ataggggatt tacccactac aaatattata
      121 gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg ctcctttttt
      181 gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg ttaaatgaag
      241 aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc aacgccagtg
      301 ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg ttcgatggaa
      361 aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt gcactaggca
      421 agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga gatgtgacga
      481 cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg gtgccgatga
      541 tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt gaggattacg
      601 atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa aagacaacat
      661 gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc gaaaatatag
      721 aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc ttaaacaagc
      781 ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg gaacgggaaa
      841 aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag tgggtggcga
      901 accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag aagatgtcat
      961 tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc gtgataaatc
     1021 tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg ttcacatgct
     1081 gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac agaatgtagt
     1141 ctttattttg gccactactg aattgcacaa gattcctgct actattctat cccgtgtgca
     1201 acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc actatatctt
     1261 agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca ggcgggcgga
     1321 aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga cacagggaaa
     1381 tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat cagccttgga
     1441 tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt gcttgaatct
     1501 tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc actatttaag
     1561 agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag tctttgtaga
     1621 aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa cagtgagttt
     1681 agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga ccgtccgttt
     1741 ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa ttgctacgct
     1801 gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg ttcctaaaca
     1861 agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg tcgatcgcaa
     1921 taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac gtcaaaatct
     1981 aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc cggacaaggc
     2041 tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc ttgcttttga
     2101 gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca tgtttggtaa
     2161 tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca tggaggaatg
     2221 gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg aaaaagaagt
     2281 agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga aggtagagga
     2341 agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat ttattattat
     2401 ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga ctggccgcta
     2461 tattatggca gccttttggg caattttact ctttagaaat ttccgagtca gttatgtgat
     2521 gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc cctcagcttc
     2581 cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta tatttataga
     2641 atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt aaaagttttg
     2701 aaaaagaaag gaactatcat gtcagtatta gagatcaaag atcttcacgt tgagattgaa
     2761 ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat tgccgctatc
     2821 atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg aaatccaaac
     2881 tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga gttggaagtg
     2941 gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga aatccctgga
     3001 attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaaga tgatgagaag
     3061 atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct taacatgaaa
     3121 gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa aaaacgcaat
     3181 gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga gattgactca
     3241 ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat gcgtggtgaa
     3301 ggttttggtg ctatgatcat tactcactac caacgtcttt tgaactatat cacacctgat
     3361 gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga attggctgcg
     3421 cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga ctacaaggaa
     3481 gaattgtaat tccctcgtat cttttaggag aagtaaatga ctagagaaaa tattaaactt
     3541 ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa agcttttgac
     3601 aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg ttggaatctg
     3661 ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt cacagcttta
     3721 gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca aactccagtt
     3781 gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga agaaattcca
     3841 gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa gttggcggct
     3901 taccacacag cttactttaa cagtggtgct gtactctata ttccagataa tgtagaaatc
     3961 acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc gtttaacaag
     4021 catattatga ttatcgctgg taaaaattct aagattaatt atctggagcg tttagagtca
     4081 cgcggtgaag gaagtgccaa agcaactgct aatatcacag tagaagtgat tgcacgttca
     4141 ggtgcgcaag tcaagtttgc tgctatcgac cgtctaggtg aaaacgtcac tgcctacatt
     4201 agccgtcgtg gtaaattagg caacgatgca agtattgact gggctatcgg tgtcatgaac
     4261 gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag ccatgctgac
     4321 ctcaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac tcgtgtaact
     4381 aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct tgaaaaagca
     4441 actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc agatgcccaa
     4501 caagagagcc gtgttctcat gctttcagac caagcgcgtt cagatgctaa cccaattctt
     4561 ttgattgatg aaaatgacgt cactgcagga catgcagcct ctattggtca ggtagatcca
     4621 gaagatatgt actacctcat gagtcgtgga ttggataagg caactgcaga gcgtttggtt
     4681 gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt tcgtgatgaa
     4741 atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta tgttagatgt
     4801 agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg aacctctggt
     4861 ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag ctattaacag
     4921 ttactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag cggaacgagc
     4981 gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg caggctctac
     5041 aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg cacgctttgc
     5101 tgaggaaatt ctcactgagg gagaccaggt cttgatttca gtaatggaac accattctaa
     5161 tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct atgtctatct
     5221 taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata aggttaaatt
     5281 tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca aggaaatcac
     5341 tcaattagcc caccaagttg gggcaatcat ggtagtggat ggtgctcaat ctacacctca
     5401 tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg gtcacaagat
     5461 ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg agcaaatgtc
     5521 tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatctg ctagttggaa
     5581 ggaattgcct ttgaaatttg aggctggaac gccaaatatg gcaggagcta ttggacttgc
     5641 gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc atgaacagga
     5701 attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca tttacggttc
     5761 tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc tccatcctca
     5821 cgatcttgcg acggctctgg attatgaagg agtggctgtt cgtgctggtc accattgtgc
     5881 gcaacccttg cttcagtatt tggaagtccc agcaacagct cgtgcaagtt tttatatcta
     5941 taataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa aggagttttt
     6001 caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac cattcgaaaa
     6061 atccacatca ccaagggaag ttagaagacg ctgagcaaat cagtctcaac aatccgactt
     6121 gtggagatgt catcaatctc tctgtcaagt ttgatgcaga ggaccgtttg gaagatattg
     6181 cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg acagatgccg
     6241 ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct gaaatggttc
     6301 aagggcaaaa agatgagcgt caagaccaac ttggagacgc ggcattcttg tcaggtgttg
     6361 ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt aagaaaacaa
     6421 ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta ttagaaatga
     6481 agaaagaaag gatattatgg ctgaagaaag agtagaacca aaaccaattg accttggtga
     6541 atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa aaggactcaa
     6601 cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga tgttggagtt
     6661 ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg gagcagactt
     6721 gtcagagatt gactttggtg acttaatcta ctaccaaaaa ccatctgaca aaccagcccg
     6781 ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg ggattccaga
     6841 agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag tggtttacca
     6901 caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag attccgcact
     6961 caaggaatac ccagacttat ttaatcaata ctttgcgaag ttggtaccgc cgacagataa
     7021 caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct acgtgccaaa
     7081 aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg aaaatatagg
     7141 tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact acgtagaagg
     7201 atgtacagca ccaacatatt caagcaatag cttacacgct gccattgtag aaatttttgc
     7261 tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata acgtctataa
     7321 cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga ttgatggaaa
     7381 cttgggtgcc aaaacgacta tgaaatatcc atctgtttac cttgatggag aaggagcgcg
     7441 tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca cgggtgctaa
     7501 gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca tcgctaaagg
     7561 tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta agaaatctgt
     7621 ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag atactattcc
     7681 atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag tatctaagat
     7741 ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg aggcaactga
     7801 aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg aatacgcagt
     7861 tgagctgaac cgcttgatta gctatgaaat ggagggatca gttggataaa atttgatttt
     7921 atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg tatggttact
     7981 gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg aggctagctt
     8041 cctagtttgt tctttgattt tgagtattag atttactcaa aatcaaggat tttgaagatg
     8101 aacttgtatc aaaaaatcgc ggtttaaaat cgcgattttt tataatttct cgttaacaaa
     8161 gcggacaaac tgattccacc aaacttttaa gaagaaggct ttttcaattt tcttgtctgc
     8221 taccatttcg aaactagggc gctctgtggt gatgtaacct tgaccaatca agtccttgtc
     8281 ttcataagtc aaatggccaa ccactgttcc agcttcaagt ggtgctggga ttgctttgga
     8341 atcaggtgtg aattgaacag attgggaaga ttgattccca acacgttcga ttagatagat
     8401 atcctctgga gccactgcag ttactgtatc ttcttttcca tcttgtacag gggctttgct
     8461 atcttgatag gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag aagaaatata
     8521 atccattagg gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt gatgatctgt
     8581 atttaaaaca actgtgatga ctctcatgcc tttttcgaca gtagtaccaa caaaagactc
     8641 tccagcctta tctgttgttc ctgtttttag cccatcaaaa ccaccacggt aagcaggcat
     8701 accttctaac atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag aagaaggttt
     8761 tttggtgatt tctaagactt gtgggtattt tttgatgagg ttgcgagcaa cgatagcgac
     8821 atcataagca ctaagcttat tttcctcatc ttttttagaa cctgggtaaa tgttatcccc
     8881 tagagtttca ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc
     8941 caagagtttt gcccgcatca tatcgacgaa atctttttct gagccagcaa ttttctcagc
     9001 tagggcaata gcggcgctgt tggcactaga taccagagtt gcttcaagca actcttcgac
     9061 agtataatta cgggcctcca taggaatatt actggcttca gaatttgtcg tcaattgata
     9121 aggataatca gaaatatcta caggagtgga gagggtaata cttccgtttt ccaaagcttc
     9181 atagaccaga taaacagtaa tcaattttgt tatggaagca atttcgacag gttgcgttgc
     9241 atccttctca tagagaattt taccagtatt tgcctcaaca gcaatcgcat gtttagcggc
     9301 aatggtaaaa tcttgagcaa cagcagtaga agcaccccct aaaagagaga cagttaacaa
     9361 agttaaaaat atttttttca tagtagtctt attctatcat aaagaaaaaa aatattcttg
     9421 ctttaataat tcatctgtta agctttttga aaatatggta aaataaagtg agggaggtaa
     9481 ctcatgtttc gtagaaataa attatttttt tggaccacag aaattttact cttaaccatc
     9541 atcttttacc tatggagaca gatggggtct ttgattaacc cttttgttag cgtgcttaat
     9601 acaattatga ttccattttt attaggaggc tttctttatt atttgacaaa ccctattgtt
     9661 actttcttaa ataaagtctg taaactcaat cgtttgcttg gtattttaat taccttgtgt
     9721 actttggtct ggggaatggt cataggtgtt gtctatctct tacctatttt gattaatcag
     9781 ttatctagtt tgattatatc tagtcaaact atttatagtc gagtacaaga cttaatcata
     9841 gacttatcta attatcctgc gctccagaat ttggatgtag aagctacaat tcagcagtta
     9901 aacttatcct atgtggatat tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg
     9961 agcgtcttgt cagctcttat cagtactgtt ttgattttga ttatgactcc agtttttttg
    10021 gtttatttct tattagatgg acataaattc ttgcccatgc ttgaaagaac gattctaaag
    10081 agggatcgct tgcatattgc aggcttatta aagaatttaa atgcgacgat tgctcgctat
    10141 attagtggag tttcgattga cgcaatcatt ataggttgtt tggcttatat tggctatagt
    10201 attattggtt taaaatatgc tttagttttt gccatttttt ctggtgtagc caatttaatt
    10261 ccttatgtgg ggccaagtat tggtttgatt cctatgatca tcgcaaatat attcactgat
    10321 ccccatagac tgctgattgc agtgatttat atgcttgttg ttcagcaggt agatggcaat
    10381 atcttatatc ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac gattttagtt
    10441 ttacttttgt tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc agtgccaacc
    10501 tattctatct tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata
    10561 atgaaagaac gagaaagaga attagctaag taaaagtcag gagaaccctg atttttcttt
    10621 actggaagtg gcctttagat tagaagactg aaaataagtt aaagtcttaa actaattttc
    10681 acagctaaga atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg gaattctgtg
    10741 tcagggtaag ttccactggt tttcatagtc tattaaagtt cgaatgaaac ctatttatag
    10801 tagattgaaa ctagaatagt acacctctaa ttctaaaac
//
LOCUS       DAVKLW010000066        10675 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKLW010000066 DAVKLW010000000
VERSION     DAVKLW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.066"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            264..932
                     /locus_tag="VW324_001763"
     CDS             264..932
                     /locus_tag="VW324_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9727342.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            892..1569
                     /locus_tag="VW324_001764"
     CDS             892..1569
                     /locus_tag="VW324_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9727343.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1570..2328
                     /locus_tag="VW324_001765"
     CDS             1570..2328
                     /locus_tag="VW324_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9727344.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2340..3201
                     /locus_tag="VW324_001766"
                     /pseudo
     CDS             2340..3201
                     /locus_tag="VW324_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3281..3928)
                     /locus_tag="VW324_001767"
     CDS             complement(3281..3928)
                     /locus_tag="VW324_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727345.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3925..4623)
                     /locus_tag="VW324_001768"
     CDS             complement(3925..4623)
                     /locus_tag="VW324_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727346.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(4633..5385)
                     /locus_tag="VW324_001769"
     CDS             complement(4633..5385)
                     /locus_tag="VW324_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727347.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(5385..6149)
                     /locus_tag="VW324_001770"
     CDS             complement(5385..6149)
                     /locus_tag="VW324_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727348.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(6245..6650)
                     /locus_tag="VW324_001771"
                     /pseudo
     CDS             complement(6245..6650)
                     /locus_tag="VW324_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6830..7462)
                     /locus_tag="VW324_001772"
     CDS             complement(6830..7462)
                     /locus_tag="VW324_001772"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9727349.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKDDQENWQEG"
     gene            complement(7496..8197)
                     /gene="pyrF"
                     /locus_tag="VW324_001773"
     CDS             complement(7496..8197)
                     /gene="pyrF"
                     /locus_tag="VW324_001773"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9727350.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            8518..8610
                     /locus_tag="VW324_001774"
                     /pseudo
     CDS             8518..8610
                     /locus_tag="VW324_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8932..9645)
                     /locus_tag="VW324_001775"
     CDS             complement(8932..9645)
                     /locus_tag="VW324_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9727351.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(9638..10471)
                     /locus_tag="VW324_001776"
     CDS             complement(9638..10471)
                     /locus_tag="VW324_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727352.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKLRVDNLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
BASE COUNT         3523 a         2180 c         1745 g         3227 t
ORIGIN      
        1 tctacatgac cttctgcatt gcttgttttg tatgacataa aatgtcctcc ttaataagta
       61 agcgtttact ttgtgtatta caaaaatatc ttaactcttt ttcaaaactt ttaaaatatt
      121 ttgtttggaa atttcagaaa ttttatgtct atgataaaaa tccttataac ggcaataaaa
      181 aatagatatt atccaaagaa gattttaagt gctacaataa ctgtattatt tctagatggg
      241 aggttctatt tttggattga tccattgttg aacaatatct accactatat caaaaggcat
      301 tctttctgac cttgcatatt gcagtttggg gaattttggg atcctttctg ctcggtttaa
      361 tcgttagtat catccgacat tatcgaatcc ttgttttgtc gcaagtagcg acagcctaca
      421 ttgaattgtc acgtaatacg ccccttttga ttcaactctt ctttctctac ttcggtcttc
      481 cccgaatcgg gattgtccta tcttcagaag tctgtgcaac gcttgggctt gtctttttag
      541 gagactccta tatggcagaa tctttccgaa gtgggctgga agccatcagt caaacccagc
      601 aggagattgg cctcgctatt ggtctgacac ctctacaggt cttttactat gtggttcttc
      661 cgcaagcaac agcggtggca ctcccctcct ttagtgccaa tgtcattttc cttatcaagg
      721 aaacctctgt tttctcagca gtggctttgg ccgacctcat gtacgtcgcc aaggatttga
      781 ttggtctcta ctatgagaca gacattgcgc tagctatgtt ggtagttgct tatctgatca
      841 tgctgctacc catctcactg gtctttagct ggatagaaag gaggctccgc catgcaggat
      901 tcgggaatcc aagtactctt tcaaggaaat aatctcctga gaatcttaca gggattgggc
      961 gttacgattg ggatatccat cctgtctgtc ctcttatcca tgatgttcgg aacagtcatg
     1021 ggaatcatca tgacctccca ttctagaatc atacgatttt taacacgatt gtatctggaa
     1081 tttatccgta tcatgcccca gctggtgcta ctcttcatcg tttactttgg cttggctcga
     1141 aactttaata tcaatatctc aggtgagact tcagctatta tcgtttttac cctctgggga
     1201 acagctgaaa tgggagactt ggtacgtgga gctatcactt ctctccctaa acatcagttt
     1261 gaaagtggac aggcactcgg cttgactaat gttcaacttt actaccacat catcatccca
     1321 caagtcttga gaagactgct accgcaggct atcaatcttg tcactcggat gattaaaacc
     1381 acttcattag ttgttttgat tggggttgtg gaagtgacca aagttggaca acaaatcatc
     1441 gatagcaatc gcctgaccat cccaactgct tcattttgga tttatggaac cattctagtc
     1501 ttatatttcg cagtttgcta ccctatttcc aaactatcca ctcacttaga aaaacattgg
     1561 agaaactaaa tgtctgaaac tatcttagaa atcaaggaac taaaaaaatc cttcggagac
     1621 aatcccatcc tccaaggact ttctctagaa atcaaaaaag gggaagttgt tgtcatccta
     1681 gggccatctg gttgtgggaa aagtaccctc cttcgttgcc tcaacggctt agaaagtatt
     1741 caaggtggag atatttttct ggatggtcag tctatcgttg aaaataaaaa aaattttcac
     1801 ctagttcgcc aaaagattgg catggtcttt caaagttatg aactctttcc ccatctggat
     1861 gtcttacaaa acctcatcct aggccctatc aaaactcaag gaagggacaa gaaagaagta
     1921 acggaagaag ctttgcaatt actagatcgt gtcggtttgc tggataaaca acatagcttt
     1981 gcccgtcaat tatctggtgg acagaagcaa cgtgttgcaa ttgtccgtgc cctcctaatg
     2041 catccagaaa tcatcctttt tgacgaggtg actgcttcgc tggatccaga aatggtgcgt
     2101 gaggtgctgg aacttatcaa tgatttggcc caagaaggcc gtaccatgat tttagtaacc
     2161 cacgaaatgc agtttgccca agccattgct gaccggatta tcttcctcga ccaagggaaa
     2221 atcgctgaag aaggaacagc tcaagccttc tttaccaatc cgcaaaccaa acgagcccag
     2281 gaatttttaa acgtctttga ctttagccaa tttggctcat atctataaag gagattctta
     2341 tgaaactatt caaaccactc ttaactgttt tagcacttgc ctttgccctt atctttatca
     2401 ctgcttgtag ctcaggtgga aacgctggtt catcctctgg aaaaacaact gccaaagctc
     2461 gcactatcga tgaaatcaaa aaaaggcggt gaactgcgaa tcgccgtgtt tggagataaa
     2521 aaaccgtttg gctacgttga caatgatggt tcttaccaag gctacgatat tgaactaggg
     2581 aaccaactag ctaaagacct tggtgtcaag gttaaataca tttcagtcga tgctgccaac
     2641 cgtgcggaat acttgatttc aaacaaggta gatattactc ttgctaactt tacagtaact
     2701 gacgaacgta agaaacaagt tgattttgcc cttccatata tgaaagtttc tctgggtgtc
     2761 gtatcaccta agactggtct cattacagac gtcaaacaac ttgaaggtaa aaccttaatt
     2821 gtcacaaaag gaacgactgc tgagacttat tttgaaaaga atcatccaga aatcaaactc
     2881 caaaaatacg accaatacag tgactcttac caagctcttc ttgacggacg tggagatgcc
     2941 ttttcaactg acaatacgga agttctagct tgggcgcttg aaaataaagg atttgaagta
     3001 ggaattactt ccctcggtga tcccgatacc attgcggcag cagttcaaaa aggcaaccaa
     3061 gaattgctag acttcatcaa taaagatatt gaaaaattag gcaaggaaaa cttcttccac
     3121 aaggcctatg aaaaaacact tcacccaacc tacggtgacg ctgctaaagc agatgacctg
     3181 gttgttgaag gtggccatta actcttaaaa gggactggat tttaagctcc aatccctttt
     3241 taagatttta cctataacat cctgagtcta tctaagatgt tcaatctgaa cacagtgtac
     3301 atactttatc ttctattgca tatactttat cacataagat acgaatatcc tcttcactat
     3361 gactagcaat caaaattgtt gtcccttttt cactagagag ctttctaaac aatgttctca
     3421 tattttctac acttgattta tccaaggcat tcataggttc atctagtaaa agaatagagg
     3481 gattctccat aattgcttga gcaatcccta gctttttcct catacctagc gaataagttt
     3541 taactttctg gtctttttgc tcatatagac caactatttt cagtgtatca ttgatttcct
     3601 gattaccaac tactcctcgt atgcttgcca aatattgtaa attcttaaag ccactataat
     3661 aatttataaa accaggttct tcaatcaaag ctcccaaatt agctggaatt tttctctcag
     3721 gaacaatatt ttccccattg attaacactt ctccataaga cggactatat aaaccagcta
     3781 ttaatttaaa caatacactt ttccctgagc cattcgcacc agtaattcct ataatttccc
     3841 cctgtttaca actaaaatta aggttttgaa aaacacatgt tttttttaat ttcaactcaa
     3901 tattttttaa tatgattatt tcattcattc tataaacctc ctcttttgac gagtgaaata
     3961 gaaaatactt ttaaaaagaa agactaaaaa tagcaactga agaaataaat ctcgtcctat
     4021 atctccattc cctcgattca aaatataaaa tagataatta gttcgatttc ctacaaatag
     4081 accaccaaac acaatcatga gtaaaaagaa actaacgcaa gcaaagttca tttgtacatt
     4141 cgctcctagt gcgtataaca taaagcaaaa caagactaac acacaagcat tcaaagaatc
     4201 aactataaac aaaactccta tatcccagtt tggtaactgt aacataaatt gaatcactag
     4261 aacttttacc atgattaaaa tgagtccata aagaaaacag gtccaaatct gatacctaat
     4321 ccagagctta atagaagaaa atcgaatcac ttccagaaag aaatcggatg ctcttctacg
     4381 atatacaact gctagaaaaa atgtatgaat aataaaccaa tgaagcaacc atatagataa
     4441 gactaaaaaa gagttttgac tcagattagg cccttttaaa atttctataa caagtctatc
     4501 atttccttct aaccaatacc ataatcctaa acaaatcaat atccaaaaaa gaacataacc
     4561 ccaaaagtac ttggtataga ttatctgatt caacctccac atgtgactac ttaactttcc
     4621 catttccaac ttttacactc tcctccagtt tctatgaaca gaaaatacag tcaatggaat
     4681 tatgatagat agtaatgtaa gccaataaat caatgcatac ggattggaat ttaccatata
     4741 ctgaaaaatt ggcaataatc caattatccc attgtccaac tgataaatcc agaataagga
     4801 ttctacaaat aaaaaagtaa acagaaatac taagctcatg tttgattttt taaaccaaga
     4861 aaaactgcta atagtaagta aggataaaaa gaaatagtat gctatataag agaaaaaaaa
     4921 tcctataaag aaactagcat tgtttgcaat acttatacca taaaattctc ttaaaaaatc
     4981 aacctccttt atctccaaag agaagctaaa accattacta aatgcaatta gaaaaatcaa
     5041 taaaaataaa gtcgccgtcc aaatccccgt actaagagct gctaatttga aactaaaact
     5101 ggtaaagtgc ttaattgatt tcagacgaat gcgacactcc aacctattaa aatagttatt
     5161 catcaaataa aaaaagaaca atatatatgt gaacggaaag caatatactc cagtcgtcat
     5221 atcttgaagt aaaactaaga tccattctaa tacatttgga tggattgaat attggcgaca
     5281 gcgcaataaa tatactgtac tagataaaac acaggatagc agtaatataa aataaaccaa
     5341 tactgataaa aaatcttttt tgtaaaattg aacaaattgt ttcattatac gtagtcctct
     5401 gaatgtagaa aaaatgtacc ataaacaacc aaacaactaa caaataaaat aaaagcaaga
     5461 tgcccactaa ctaaggaaag actgatatct ttctgatatc ccaaagctaa tgttgtcaca
     5521 ggttctaagt aagatagccc taaaatagcc caaaaaatac caccaaccat catataggca
     5581 actgggatga aaatagctcc tatttttttc ttcactagca aagcactagc tagtccaaaa
     5641 atagagaaca cagcgcccca aactccatac cagagagtcg tcacaagact atagagcaac
     5701 tgattagaat caaataattc ttttaaggca ccactataat ctccaatata aatttcctga
     5761 taaggagtca ctaaaagatt aattcctaat aataataaat aggggagaaa aaagactaga
     5821 aaagaagaaa taattgcagt actacccaca atagctagat acctcttttt agaaattcgg
     5881 cacaattgtg ctgttagaaa atgactctca gcatcctcta ttatctgact agaatagggc
     5941 agtgtacaga taagtgcagc tactaggcta atcggtgaaa atcctcgaat agaagaagcg
     6001 gcaaaaaaaa tcgataaacc ttcaatttta taaataccat tgaaagcaag gaaatttcct
     6061 aaactcatgc aaagaagggt caaaaataaa gacatataaa atcgaggtga ttgaacgact
     6121 cgtacaagat tacaaatgaa aaattccatc cttactcctc cttataataa aaatagggtg
     6181 tagcattctt ttttcatgct acacccacaa tcaaccatct ttaaggctta ctctgacaag
     6241 taagttaata agaatctgga ctccaagaac ctgaagtatg aattcttaca tgatttccaa
     6301 attgtggcgc catagctaat ctagtaccag aaccaatata attgtcacca cctccattat
     6361 agtacatgac aatcctagag ccagacccca atgaatatac cggggtaata tctgacccac
     6421 tataggcgcg ctacgaatag aggtatttaa ccttttaccg ccaccagtgc tgtcactatt
     6481 attaattcca gcagaggcgt tttctttctc aagctcacta gttgtatccg aaccccaacg
     6541 cggcaacgtt aaatcataat ttccaatctg tctagctaaa actgtaggga gaaacaaact
     6601 agatgcaatt gtaactacgc aaaaaaggaa tatatttttc ttcttttcat gacatcgtcc
     6661 tcttaagaag tgataaacaa gatagttgat tgatcaattt gatacatatt acattttcat
     6721 tatatcaata aaagaaagcg ttgtcaatta ttttatttaa aatattgtca tattttaaat
     6781 aaaatggtaa aagaggctcg gtagcctcgt ctctctttat cttactgacc taaccttctt
     6841 gccaattttc ttggtcgtct ttaaagcgtt ttagaagatc aaggccctct ggcgtgatgt
     6901 aaccttcttc ttgggctaga tggataagct cgctatagtt tgaaagcgtc acaagtttaa
     6961 caccagcatc tgcaaagttc ttatctgctt ttggcaattg gtagctgaaa atcgctacaa
     7021 ctccaagcac atctgctcct tctcgcttgg ctgctgctac agcttcaaga actgaaccac
     7081 ccgttgaaat aaggtcttca accactacca ttttttgacc ttgagctacg cgaccttcga
     7141 tttgattacc agctccatgg tcttttggtt tgctacggat gtaggcaaaa ggcaagtcca
     7201 tcttatcagc aataatggct ccgtgtggaa tccctgctgt tgcagttcct gcaatcactt
     7261 ctacttcagg aaaggcttct ttgatagctt ccacaaaacc attttcaatt agggtacgag
     7321 tttctggata ggctagtgtc acacgattat cagtgtaaat cggtgacttg ataccagatg
     7381 cccaagtgaa gggttcctct ggtttgaggt aaacggcttg gattttcaag aggtggctag
     7441 cgatatcttt agcaagtgtc atggtattct ccttttattt ttctaatcta gttctttaat
     7501 tccagtcctg tgtccattca tccttgatgg catgataagc tgcaacagga tcctcagctt
     7561 gggtaatggg acgtcccact acgatatagt cactgccgat ttgataggca tcagcaggtg
     7621 tcatgactcg tttttgatct ccaactgcaa caccagctgg acgaatccct ggtgtcagac
     7681 agataaaatc tggattggta gcctgcttga tgacttgtac ttcctgagcc gagcaaacaa
     7741 caccatccaa gccagcttca gctgtcttct tggcatagtg aatcacagac tcttgcagac
     7801 tggtttggat attttgaaac tcctgcatct gagcttctga cgttgatgtg agctgagtta
     7861 cagcgatcaa tttggcttga cttccaagac cttcacgcgc agccttcatc atctctacac
     7921 caccagccgc atgaacattg gtcatatcca caccaagctg agataggatc ttcattgctg
     7981 acttgactgt attaggaatg tcatgaagtt tgagatccaa aaagacacta tgacccaaac
     8041 cttttaagta ggacacaatc tcaggccccg ctgcgtaata aagctccatc cctaccttga
     8101 gataaaggct ttcttctgct gggaaaagag ctaaaaattc cttgaccgcc tcaaaactag
     8161 gaaaatcaag agcaatgatt ggacgatgtt ctcgcatagg tttctccttt tacctttact
     8221 agtgagagag tctggccaac agaagccacg aaaaaaggaa cctgccaaca gcaaggttcc
     8281 acgaaaaatg ggtagtctcc accctttcac cgtctaacct tagctgcctc tctggactgc
     8341 tttaaaggtt ttttactatt ctattatttc tactagcact tgtcaagtaa aaacatggtc
     8401 acgtatgtgt tgactgctat tgaacacctc acaagactct ttccaaaaga atagttaaag
     8461 actttcactt aggacagaga aaaagagttt gcttgttacc ctttaattga acaaatggga
     8521 attctgtttt attttgcgga tgcctattca tcttggcaaa ggggaagtaa tgagaatgcg
     8581 aatggacttc ttcgtgagta ttttcttaaa ccgatttggc aacaattacc gaaaaaatcc
     8641 ttaatggtgc tttgctagct tattaatcac cgtccccaaa aatacttgga ttacaagaca
     8701 ggttttgaag cacttataga ggagatttaa aaatgttgca ttttttattg caatttatca
     8761 gttatcttat cacccaaaac ttctgcaatt agtatgagca ctattgttat ttttcttttt
     8821 tcgtacactc aaatcaaaat atagcaataa gactattctg tagaattgct gtcaaaaaga
     8881 tcagttcatt tttccttccc acttgtgttt ataatcatta aaataactca attaagaaat
     8941 tttctttttc ctaatagtta tcgtaattaa agttaataga agaacaatcc aaataagtag
     9001 tgttagatta ggaattagag tttcattcca tgaaaatccc tcccattttg tgaaaaactt
     9061 agtaaacagt cccataaaac tataatctgt tatcttcgca actgtcttat ccaaaccaga
     9121 tagcatcggt aagaaagcaa ctaaaatcat agcaggaaga ctgataacct gagctactac
     9181 ttggctcttc gcggttaaac ctatcaataa atagaataaa atgatggata aagaggttag
     9241 aagaacgatt gtaatataat taaaagtatg tacaattgta actcctaaaa taagaggagt
     9301 agttcccata atcacaaaag ttagcaaaaa aggaagaaac atcgttgata aaatgtattc
     9361 ggatccttta acaccactca acagaagagt ttgtaaattg tacttttctt tttcttcaga
     9421 caagataata gttataggac ttccaacagc caaagccaaa gaaaaaggta aacacatcat
     9481 caaaagaact aatgcctgtt gatcgttgac cttcccctgt gtttccataa gatatttata
     9541 aaaatatgtg aaagcaaaag gcactaaaac ttgcaataaa atactcttat tggaaaggat
     9601 aatttgacaa cgcaaccata ccaaagccag aaaccgttta agcatttagc tttcctcctg
     9661 ttaatgtgat aaaaatatct tctaaagtag gctcacatga atgaattgaa aaaatatttt
     9721 ccagatcagc ctgagatacc tgttctagtg atgtaaaatc aaaagttatc tgattcccat
     9781 tataatctgt aaccttaatc tttttatctt tattatatct ttgaatgagt tcagaaggag
     9841 ctccttgctc aactaatttc cctttattta ataaggcaac ataatcacat aaaagagttg
     9901 cttcattcat atcatgagtc gttagaaaaa tcgttgtccc tgctgttttc aattctaaaa
     9961 ttaactcatg aattgttcga gaagttgtgg gatctagacc tgaggtcggt tcatccagaa
    10021 agagtacagc gggtttgttg ataagagctc gtgctaaaag cattcgttgc ctcattccag
    10081 tggataattt tcctgctacc atcttgcaac tatcatataa tcctactcgc tttaacaaat
    10141 tatcaacacg taatttacta atattataaa atttactata aaaaagaaga ttgttataca
    10201 gagacatttt ctcataaaat ccacttgtat cgctaaccaa tccaattctc tttaattcac
    10261 cgcttgttaa attttgagat ttttgtccca aaataataga ttgtccttta tcggcaagga
    10321 gctgcccagt cagaatatta atcgttgtgg tctttccaga accagatggt cctaaaaatc
    10381 caaaaatctg accttcttta acagtgaaac ttatatcatt taaagctatt ttatcaccaa
    10441 aactttttga taaatggcta acttcaatca tactcattgc ccctccttta tattctacat
    10501 ttttacttaa catctattta cttagcttta tcaaataaac gtcttaaaaa aaacaataac
    10561 attaagcata taaccgttag caagattgtt tcaatattat tgataatatg atacattctc
    10621 aagcagtata aaataattat atatatgcaa atgctcatta tacctatttt tttaa
//
LOCUS       DAVKLW010000067        10623 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKLW010000067 DAVKLW010000000
VERSION     DAVKLW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.067"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(117..1541)
                     /locus_tag="VW324_001777"
     CDS             complement(117..1541)
                     /locus_tag="VW324_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9727353.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW324_001778"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW324_001778"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9727354.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW324_001779"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW324_001779"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9727355.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6528..7706)
                     /locus_tag="VW324_001780"
     CDS             complement(6528..7706)
                     /locus_tag="VW324_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9727356.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMVGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLQSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7786..8649)
                     /locus_tag="VW324_001781"
     CDS             complement(7786..8649)
                     /locus_tag="VW324_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9727357.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8652..10553)
                     /locus_tag="VW324_001782"
     CDS             complement(8652..10553)
                     /locus_tag="VW324_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9727358.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYIEKRATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3192 a         2346 c         1897 g         3188 t
ORIGIN      
        1 tgtactattc tggtttcaat ctattataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctggttt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag aagattgccc
      481 tcacgtttga ttggtgtaag agcctgagct tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa tttcttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg taaataagcc gcacgttcaa ctgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaaacttg atttttcaaa gtatactcaa taaaaataaa aaaaatccac
     1681 tacgtcaagg cgaggctaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagccaaacc aatttctcac
     1801 aatgtttcag aaacaagggt gtgctactcc aatttcagcc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag acctcctatc aagtgttcaa
     1921 aaactttgat aggaggtctt gttttgtgaa aatatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt actaatactc
     2041 ataactggga atttgtctgt gtaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttaccaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaaccac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggtag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtatagct
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggcaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatct ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatgt gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagtc ttacctacca aatcttcttc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgcact tctgtcgctg taggtgacca gagggaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatgatca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaagga ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgttaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaac actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactagcc acttcctctg aatctgcctg
     4861 atacagattt tcaattgaat ggaaatattg aagtaaaagc tgactagcct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaaggc ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 ggcagggaac ttgtaggcta ctgcccactt tggagcctta actgtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gcagattttc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcacttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcagctcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgaaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 gcggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctta tatcaatttt
     6541 tcaaaatgag ttcgaacata aataaacgat atacaagaca agatgataac accacttcca
     6601 attatcagga aagaagagag atgtacactt tgcaagactg tcataaatcc ttttgcaata
     6661 ggcataaata gaatagctaa ggtaaaaatt gtactcagta ctctcccaag aaattcgctc
     6721 tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa aaatcgtcat aaacaattca
     6781 caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg gtaatcccat cataaaaact
     6841 ccgacacctg tcaaagccag taaaatcaaa agattataaa tattagcttt aattttacta
     6901 gctagaagag ccccaatgat ggaaccaata gcccccatag ttaaaatact tgcataggct
     6961 ccttctgacc cgtaaagctg attcgaaaag ggaagtagaa attcaaaagc tgcaaaaaag
     7021 aaattaacgc tggaagctac cagcaaaagg aagaaaattt cttgctgatg ccagatatag
     7081 tgtaacccat ccttgatatc tacaaaaata tctctcccag taaaagcctt tttctcttga
     7141 acttttgctt cctcttttgg aaggaaagcc actagaacaa aagcaatgaa aaaagtcagc
     7201 gagtctagca gtagcgtcat atggagactt gcaaactgta aaacaaggaa ggaaagaaca
     7261 ggagagctaa cacctacaac ctgcaaaacc agctctaagc gagaattata gatcacaatc
     7321 tcatctttct ccaccacttc agttatgata gctttattgg ctgtgcgaga aaaggcaaaa
     7381 gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa ccatccagct atcattcctt
     7441 atgaaagaaa tagccagaca aagaatccca caaacaagat ctgccgtcat taaaatctta
     7501 cgacgagaaa aacggtctga aataactccg ccaaagggat tgacgagaat agatgtgacg
     7561 agctcagaaa tctgatacat tcctaaaact gtctgtccta tagtccccat agaagccaac
     7621 cagacactat ttccataatc atagagcata tttcccattt tattgatagc cccacggcta
     7681 atcaactgca ctgcatagcg attcatatta aagctcctct caaattttga aactattgta
     7741 tcaaaaccga aaggagcttt ttattttttc ccttatttga gaaaattaac ttttgacaaa
     7801 tttttcgtag tgttcctgat aataggctac ttgctctgga agacctaaca catcaaaaat
     7861 atgcatggcc tcttgcatct gcttacagcc ttctttacac tgtccttttt gatataaggc
     7921 aaaacctttt aaataatgga aaacattacg ctcataaagc ttaatacctt tgtcaataat
     7981 cttctctgta taagcctcaa aatagttggc attataaaaa gaagaatgct ctaaacaatg
     8041 ctggtaacaa ttgagggcca aaattaacac taatctctta tggcgactaa tctcttggta
     8101 aaattcctcc ctctccataa cttctctacc aatccgagtg acatagtcta catcgtagaa
     8161 actatagagg ttaccgaaaa gaatcaactc atacatggtc cattcttctg ttttgaagag
     8221 ataatctgct accttatcca aatcatcctg cttcatatca taagtcgcat ctctttgaca
     8281 aatcagacct tgtagcaaaa tccagttcag ctcaaaataa aggggagtcg tcgaactctt
     8341 agacttttca agttgttctc tttgaagctt ttgaaaacct gcaatatcgt ttgaatagta
     8401 aagtgggata atctgtgcca tcatagacac atgttcatga ttatgaaaat tccttgcctt
     8461 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8521 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8581 tgctgcttcc tttaaagaat aatttccact tgttcgaaat tcacgaaata cttttccaag
     8641 atgttccatt tttacacctg ctctgataat tcttcccact caagcatagc ttcttcctga
     8701 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8761 tccaacattt gttcagaaat ggcttggctt tgactttcta gctcttcaat ttcagcttct
     8821 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8881 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8941 ctcatttctg ctgttgctct cttctcaata tagtagtcgt aatctccaag gtagagagtt
     9001 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9061 tgactgacaa aaagcaaggt accatcaaag tcaatcaagg cattttctag cacttcctta
     9121 ctatcaatat ccaaatggtt ggtcggctca tccagaatca aaaagttatt gttttccata
     9181 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9241 ttaacatcat ctcctgagaa aaggaaggct ccaagacggt tgcggatttc aacttctggt
     9301 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9361 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc acctttgata
     9421 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc atttggacca
     9481 acgatagcga cagcattcat cttacgaaga tctaggttga taggttgtga caaaacttcc
     9541 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttca
     9601 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9661 ttttctagtt gtttacggcg agattgagca cgtttggtcg ttgaagcacg aactagattg
     9721 cgattgacaa agtcttccag agcagcgatt tccttctgtt gcttttcata gttttttgcc
     9781 tcagtagcta gcttttgctc cttcaactca acaaaacgag agtaattccc cacatagcga
     9841 tccaaggaat acttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9901 tcgtggctga cgataatgag ggcaccgcta tagtttacca agtaattctc tagccaggag
     9961 atggtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
    10021 aggagcattt tagctagagc caaacgagta ttttgaccac cagaaagctc agcaattttc
    10081 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10141 taggtaaagc cacctgcttg gcgaaaattt tcagataagc ggtcataatc tgacatcagt
    10201 ttatccaaat cctcaccaga cttttcaccc atctccagct ccatctgacg cagttgttta
    10261 tctgtccgac gtaagtcatc aaagacatga agcatctcat catagatggt attttcagac
    10321 tcaaaacggc tatcttgagc taggtaagac agagaaatat cttttttctt attgatttct
    10381 ccactagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10441 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10501 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat catacaagta
    10561 gtatagcatg tttccctaag gcattcaaga taatcgtaag tcttttagta caacttttat
    10621 aac
//
LOCUS       DAVKLW010000068        10525 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKLW010000068 DAVKLW010000000
VERSION     DAVKLW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.068"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(391..588)
                     /gene="blpI"
                     /locus_tag="VW324_001783"
     CDS             complement(391..588)
                     /gene="blpI"
                     /locus_tag="VW324_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9727359.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            870..1457
                     /locus_tag="VW324_001784"
     CDS             870..1457
                     /locus_tag="VW324_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9727360.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1384..3027
                     /locus_tag="VW324_001785"
     CDS             1384..3027
                     /locus_tag="VW324_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9727361.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            3038..4399
                     /locus_tag="VW324_001786"
     CDS             3038..4399
                     /locus_tag="VW324_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9727362.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4456..4584
                     /gene="blpC"
                     /locus_tag="VW324_001787"
     CDS             4456..4584
                     /gene="blpC"
                     /locus_tag="VW324_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9727363.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4655..5995)
                     /locus_tag="VW324_001788"
     CDS             complement(4655..5995)
                     /locus_tag="VW324_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9727364.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(6009..6746)
                     /locus_tag="VW324_001789"
                     /pseudo
     CDS             complement(6009..6746)
                     /locus_tag="VW324_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(6751..7089)
                     /locus_tag="VW324_001790"
     CDS             complement(6751..7089)
                     /locus_tag="VW324_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9727365.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7365..7694
                     /locus_tag="VW324_001791"
     CDS             7365..7694
                     /locus_tag="VW324_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9727366.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7862..8911)
                     /locus_tag="VW324_001792"
     CDS             complement(7862..8911)
                     /locus_tag="VW324_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9727367.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8908..9639)
                     /locus_tag="VW324_001793"
     CDS             complement(8908..9639)
                     /locus_tag="VW324_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727368.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9707..10117
                     /locus_tag="VW324_001794"
     CDS             9707..10117
                     /locus_tag="VW324_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9727369.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            10127..10414
                     /locus_tag="VW324_001795"
     CDS             10127..10414
                     /locus_tag="VW324_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727370.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         3488 a         2203 c         1799 g         3035 t
ORIGIN      
        1 ctttttgatt ttcttaaaaa agtatatacg tctactatct tcttaaaggt agcagtacct
       61 attttttagt ctaagatttc aataatcttg agtatctaaa atatcttaat ttcgttattc
      121 tccttgcaat aaaaagtttt actatactat ttattaactt gcagaaagca aaaaatatta
      181 gtaaataata gtttatagtt aagtttttta ttcctaccaa tccatcaact aagtaaagca
      241 tcaacgatta cataaacgat tgataatata attaaaattt tgctaactat cttattctca
      301 tgattcttag ataactttga tattttgtaa gtaagtaaat aagacagtaa attaatagcg
      361 ataataatac tatatttaag aatcataatc ttacaaagag gacataattc ctgaacctac
      421 acaaataagt gttgctgctc ccccagttat cggaccagtc gcagcagcta atagtactgc
      481 tccaatacaa ccaccgattg cagatcctaa attgcctctt cctccactaa ctatttcgag
      541 ttcttcatta tccataacag aaaattgttc catcattttt gtattcatga caaatactcc
      601 ttttttcttt ttttattttt gtcttgttgt aactttgata agtttagtat atcatcgttt
      661 tttaaaattt ttcatccaga tcttgaattg tcatcgaaac gtcttgaatt aggtttttta
      721 tttcaagcca cctctaaatg tttaaaaaaa ataatttcta atcacttttt taccattcag
      781 gaagttttaa tgactattca agatttcata aaatatgaac ttagttttat gacataatag
      841 acctatccac tatatgaaag gaattgccaa tgacttctta taaacgtaca tttgttcctc
      901 aaatagatgc gagagactgt ggtgtcgctg ccttagcctc gattgctaaa ttctatggtt
      961 cagatttttc tctagctcac ttgagagaac ttgcaaagac caataaagaa gggacgactg
     1021 ctcttggcat tgtaaaagcc gctgatgaaa tgggctttga aacaagacct gttcaagcag
     1081 ataaaacgct ctttgacatg agtgatgtcc cctatccatt tatcgttcac gttaacaaag
     1141 aaggaaaact ccaacattac tatgttgtct atcaaacaaa gaaagactat ctgattattg
     1201 gtgatcctga cccttctgta aaaatcacta aaatgtcaaa agaacgcttt ttctatgaat
     1261 ggactggagt agctattttt ctagctacca aacccagcta tcaaccccat aaagataaaa
     1321 agaatggtct actaagcaag cttccttcct ctgattttca aacaaaaatc tctcattgct
     1381 tacattgttc tctcaagctt attggtcact attatcaata taggtggttc ttactatctc
     1441 caaggaatct tggatgaata cattccaaat cagatgaaat caactttagg aatcatctca
     1501 gttggtctgg ttatcaccta tatcctccaa caagtcatga gcttctccag agattatctc
     1561 ctaaccgttc tgagtcagag attaagtatt gatgtgattt tatcctatat tcgccatatt
     1621 tttgaacttc ccatgtcttt ctttgcgaca cgtcgtacag gagaaatcat ttcacgattc
     1681 acagatgcta actctattat agatgccttg gcttctacca ttctttctct ttttctggat
     1741 gtttctattc tgattcttgt aggaggcgtc ttactggcac aaaaccctaa tctcttcctt
     1801 ctttctctta tttccattcc tatatacatg ttcatcatct tttcttttat gaaacctttc
     1861 gaaaaaatga accatgatgt catgcaaagt aattctatgg ttagctctgc cattatcgaa
     1921 gatatcaacg ggattgaaac tataaagtcg ctcacgagtg aagaaaatcg ctatcaaaat
     1981 atagacagcg aatttgtaga ttatttggaa aaatccttta agctcagtaa atattctatt
     2041 ttacaaacga gtttaaagca gggaacaaaa ttagttctga atatccttat cctatggttt
     2101 ggcgctcaat tagtcatgtc gagtaaaatt tctatcggtc agctgattac ctttaacaca
     2161 cttttttctt actttacaac tcctatggaa aatattatca acctccaaac caaactccaa
     2221 tctgcgaagg tcgctaataa ccgtttgaac gaagtctatc tagtcgaatc tgaatttcaa
     2281 gttcaagaaa accctgtcca ttcacatttt ttgatgggcg atattgaatt tgatgacctt
     2341 tcttataagt atggttttgg acgagatacc ttaacagata ttaatctcac gattaaacaa
     2401 ggagataagg ttagcctagt tggagttagt ggttctggta aaacaacttt agccaaaatg
     2461 attgtcaatt tctttgaacc ctacaaagga catatttcta tcaatcatca ggatattaaa
     2521 aacattgata aaaaagtctt gcgccgtcat attaattacc taccacaaca agcctatatc
     2581 tttaatggct ctatcttgga aaacttaact ttgggcggta atcatatgat tagccaagaa
     2641 gatattctaa gagcttgtga attagctgaa attcgtcaag acattgaaag aatgcctatg
     2701 ggctatcaaa ctcagctctc tgatggagct ggtctatcag gaggacaaaa gcaacgaata
     2761 gccctcgctc gtgctctttt aactaaagct cctgttttaa tactagacga agctactagc
     2821 ggtcttgatg tcttgactga gaaaaaggtt atagataatc ttatgtctct aactgataaa
     2881 accattctct ttgtagccca tcgtctcagt atagccgaac gaaccaaccg tgtcattgtt
     2941 cttgaccagg ggaaaatcat tgaagttggt agtcaccaag agttaatgca ggcgcaaggt
     3001 ttctaccatc atctattcaa taaataagga gaatgtcatg aatcctaatc tttttagaag
     3061 cgtcgagttt tatcagagac gttaccataa ctatgcgaca gtgttcatta tacctctttc
     3121 attactattt actttcatct tgattttctc ccttgttgcc acaaaagaaa ttactgttac
     3181 ttcccaagga gaaatcgccc ctacaagtgt cattgcctcc attcagtcaa ccagtgataa
     3241 tcctatccta gctaatcatt tagtggcaaa tcaagtagtt gaaaaagggg acttactcat
     3301 caaatactct gaaacaatgg aagaaagtca gaaaactgcc ttagcaactc aattacaaag
     3361 acttgagaag caaaaagaag gacttggaat tttgaaacaa agcttagaaa aagcgactga
     3421 tcttttttct ggcgaggatg aatttggcta ccataatacc tttatgaatt ttactaaaca
     3481 atcccatgat attgaactgg gtatcacaaa gactaacacc gaagtttcaa atcaagctaa
     3541 tctttccaat agcagttcat cagctattga acaagaaatt acaaaagttc aacaacaaat
     3601 tggagaatat caagagttga gagatgctat cataaataac agagcacgct taccaactgg
     3661 caatccgcac cagtcaattt tgaatcgtta tcttgtagcc tcacaaggac aaacacaagg
     3721 aactgcagag gagccatttt tatctcaaat taatcaaagt attgcaggtc ttgaatcatc
     3781 tatcgcaagc ctcaaaattc agcaagctgg tatcggaagt gtagcaactt atgataacag
     3841 tttagcaacc aaaattgaag tactccgcac tcagttttta cagacagcct cacagcaaca
     3901 actaactgtg gagaatcaat taacagaatt aaaagtacaa ctagatcaag ccacacagcg
     3961 tttggaaaac aataccttaa cctccccaag taaaggtatc gttcatctga acagcgaatt
     4021 tgaaggtaaa aatagaattc caactggtac agaaattgct caaatattcc ctgtcatcac
     4081 agatacaaga gaagtactaa tcacttacta cgtatcttct gactatctac ctctactaga
     4141 taaaggacaa actgtaagat taaaactgga gaagattgga aatcacggca ccaccatcat
     4201 cggccaactt cagacaattg atcaaactcc taccagaaca gagcaaggaa atctctttaa
     4261 attaaccgct cttgcaaaac tatctaacga ggatagtaaa ctcatccaat atggcttaca
     4321 aggtcgcgtc actagtgtaa ctacaaagaa aacatatttt gattatttca aagataaaat
     4381 tttaacccat tctgattaat tttcagataa cactctataa ctatttatta tcttatcaaa
     4441 aaggagaatc ataacatgga taagaaacaa aacctaactt catttcaaga actaacaact
     4501 accgaactca accaaattac aggtggagga ttgtgggaag atttattata taacattaat
     4561 agatatgctc attacatcac ataagaactt catcatccaa tacaactata aaaaaataag
     4621 accgagaaac aagtactctc ggtcttattt ttcatcattc tgtatgtatc acagtaagta
     4681 cctgacgaaa gacttgattt tgacaggtgg tatttagact ggtattagga tggctttcca
     4741 caatcttcat gacggtatag agaccaactc ctctctcctc ccctttagaa ctggctccaa
     4801 aggagaagat ttcagaaata tcgatgccct cttctttgat ggagttttcg atgataaagg
     4861 tctcctgtgc tccatttttt aaaaaggcga ttgaaacatg aggttgacta gcttccacac
     4921 tggcttcaat agcattgtca caaaggatag acacaatggt tagaaaatca agtagactca
     4981 tcccctcgac ctgaatctcc tcaggaactt cgacattaaa gacaatgttc ttatctctgg
     5041 cttttaaaaa tttccctgct agaagacttt tgagggcttt atcacgaata tttaccaatc
     5101 tgcccaggtc atatttattg ttctgcaatt tctgactgga atcctttaag acggagccat
     5161 agacctcttt tatctgctcc atatcctcct cttcaatgcc cagacgtaag ctagtcaaga
     5221 ggttggtata atcatgacga aagctccgta cttccttgta aagctcctct atatgccgac
     5281 tatagcgttc catatctcta tagcgcaggg cctgctcttg ttccaatctc tcatagagtt
     5341 tttccttcaa ataggtatcc aatttcttga taacccccat aaaaaagagt aggtaaaaga
     5401 ctaggatgag atggcgaaca gtctttgatt gaatactttg ttcatattca aaaaaagaca
     5461 gactttccat gactagatag tagccaccca ttatccagtt aatctgagtc agggactttt
     5521 gaaaggcttt atcgagaatc tcctttctca agctagtaaa atcgtagtcc aaccatttca
     5581 aaaaagctag agaaatgaag aaattgaaaa ttattataca taacccagta aatgagtagc
     5641 catcatatac ttgcccttgt cccaaaaatg gaagcacaaa ataggagact cctctataaa
     5701 agagattcac caatatcatt ggaaagagac cataaaagaa aaggagtttt ttaggaagcc
     5761 ctctcaataa taagaaagat aagcctatgc cgtacaaggg ttccataaaa taagataggt
     5821 aaacatttcc tactatatag ctaatcatca caaaaacaaa ggccaacagt atcttaaaaa
     5881 gaaaggcctt aaaaatcctc tcgaaagtaa gatcaattcc atccacctta aagaagatga
     5941 caatttctag tccattagta acaagtgtat acaacaatat ccaagcaatg ttcataaatt
     6001 ctcctagctc agtgtaattt attgatggcc tcagacactt ccctgacctt ataacgggcg
     6061 attagacaac ttccaccatt gggaaagaaa agcagttttt ctttcttatc caaatgcacc
     6121 acatttgcag gattgatgag aaaagagcgg tggcactgca agagacgggg ctcctgcttg
     6181 aaaacctcct ctaaactcgc tgtaaattcc agcctgtctg tcttggtata gagaataaca
     6241 cgatgggctc tgggcgacgt ttcgagatag taaacctctt taaaaggata ctgaaattgg
     6301 gcaaattttg atttaaagta aaagcaatct tccgccagac ttttactatc ttgactattg
     6361 gcatagagga gggctgtctc gatccgagat tcaaactcct ctgctgacaa ggccttatca
     6421 atgtagtcca aagcagacac ttggtagcga aaagacaggg gcataaactc cgagtgagtc
     6481 gtcacaaaga caatcagggc ataaggatcc cgatcccgaa tctttctagc cacttccagt
     6541 cccttcatct cttcatttcg aatctcaata tccaaaaaga atagctgatg ggcccccttc
     6601 tcatgcactt cagccagcag ttggtccggc ttgccaaata cctcaaaaga gctaggaatg
     6661 atatgatgtg ctttcaaaag tttctcaatc gtcgtttcaa ttctagtcta ttgggaaaaa
     6721 tcatcctcta aaacaaatat tctcatcttc ttactctccc tctttcaacc atttttgacg
     6781 aatttctcta tagcgacgtc tggcatactt gacagcgtat tgaccttctt ctccgagaaa
     6841 aaggattctt tcttgaaaat caatcgattt taatttatca agattaacca aacaatttcg
     6901 atgacatctc atcaaggttt ccccacattg gttctcaaga ttacttaaat tttgaagcat
     6961 attaaaacta gcttcttctg taacaatctg tacggtatgg gctttagttg gatgtgtttg
     7021 gatatagtag atatcgtcga tgtttacttt atagactgtt ttctgcgtct gaataatcat
     7081 atatttcatt ggattctcct cctaagaaaa gaataacaga aaaggtctag cccaagcaca
     7141 cccaatccta aacagtcttt gaaaactcct aaaaggatta ttttctacaa catcatgatg
     7201 agcttacaac tattttatca taaaattcac tcagcgcaat tcaagacatt tcaatgacaa
     7261 ttaaagattt gaataaaaaa aaagaattaa aagtgataaa ctaacagtat caaaacacag
     7321 ttgccagaag taagactggc acccagatta aaggaggcat tcccatgaca gatacagacc
     7381 ccatcaaaag agctcatact ttgattactg acttaaacaa agcctaccaa gcatgcaaac
     7441 aggcaagcgc tgacgacgtc cgctttcagg agcaattaaa ctctattctt ggttttctag
     7501 ccaaggctga aacagtggat aatcgattct tgattgaact agaaaaattt taccagactt
     7561 ccagtcttct catgggactc agcgctcttg atccagatgc tccaactcgc gccgcttggc
     7621 gggcctatga ccgcttccac tttgaccaag tcaagaccaa gttaatactc aatgaaaatc
     7681 aaagagcaaa ctaggaagct agccgcaagc tgtacttgag tacggtaaga cgacgctgac
     7741 gtggtttgaa gagattttcg aagagtataa gtctctacgg accaaccatt atcctgtagt
     7801 aacaaaaaag aagttgagac agactgaact caacttcttt tttagtgtcg tatcagcaat
     7861 gttagtcctg catctgacgt tttacctgat aaggcaaata caagactagt aaaaccaaac
     7921 cagctcctag taaggctaga agggctagtt tttcttggaa ggtaatcaac ccaacaacta
     7981 attccacaag taaaacaaaa ctggtcaatc ctcgaactac ctcctgtaag cctttttcct
     8041 tttgcccctt gtccagcgga aagagttggg tcaaatactg gtagtcaaag gcatgataga
     8101 gggccagcaa ctggaagagc aagaggtagt taaagagaac taccactgct gtcgcaatcc
     8161 aagcttgctc gataaaaacc tgcgccagta cggaaagtaa gagaagacga agactgagag
     8221 caaagaggtc gccatttcgc agataagaac gcagatagag attttgccaa atcttcccag
     8281 gcaccttctg aacagccttt aagataaagt ccagataggc acgacgctta acgctgtttg
     8341 aaattccctt gacctgcgta aagagggcaa agaaacgaag caagacttgc ttacgcttgc
     8401 tttcttggga aatgacatag tcccaatcca gtccagtttc agtgaaaaat ttgctggcct
     8461 tttgacgaaa gtggaaatat tttcctaccc ccaataaaag cacatagagc agaaaaactg
     8521 gcaagccata acccattgct aaaaataaag gcgcaaataa cagcaagaaa agggtctgta
     8581 caaagagcca aaagactagg gaaatgccag tttgacgctt gagatggagc ttgatttcct
     8641 cttctccaac taagagaaag agcttgtctg gagcctccat ataggtggca attcctcccc
     8701 aaagtaaaag taaaacagac gtaattccta caaacaaaag gataggccaa tgattttcag
     8761 gaaaatgttg taagagttga ctgtactggt aggctagaaa gcccaacagg acaagcagga
     8821 acaagacaaa gtggtcattg agcacatagc gcagataacc aagacactcc ttacgaaagg
     8881 cctgctttct ctttaaaaac aagtctttca taggtcctcc tcttttgtca gagccaagta
     8941 aatatcattc aaactagcct caggcatatc aaaggcttcg cgtagttgca ggagattgcc
     9001 tttggcacgc acctctccct tgtgaagaat gacaaaggca tcacacatct tctccgccga
     9061 atccagcacg tgggtactca tgagaataga cttgcccttt tgcttctcca cttccaaaag
     9121 ctgaatcaaa tcagaaatag ccagcggatc aagaccaagg aaaggctcat ccacgatgaa
     9181 aagacttgga tccgccacaa aagcacagat aatcatgacc ttctgcttca tcccttttga
     9241 aaaatgaaca gggaaccagt ctaatttctg ttccaaacgg aacattttta acaagggctc
     9301 tactcgttcg aaagccactt tttgctcaat accgtaagcc atagcaaccg tttcgatatg
     9361 ctctctgagg gtcaattcct catacagact aggcgtctca ggaatgtagc caatctgctt
     9421 gcggtagcta gtcgcatctc cttgcagagt caggccattg atattgatgg agccactata
     9481 aggtgccaac agaccgataa tctcattgat cgtcgttgat ttcccagcac cattgagacc
     9541 aatcaaaccg accaactgcc cactttcaac agtaaaggac acatctttca aaacaggaac
     9601 atgaacatag ccacctgtca ggtttttaat ttctaacata ttttctccaa atctggtata
     9661 atgtagctat attatatcaa aattcagtac agtagaggta gattttatgt cagattgcat
     9721 tttttgtaaa atcatcgcag gggaaattcc tgcttcaaaa gtatatgaag atgagcaggt
     9781 ccttgccttt cttgatatct ctcaagtaac actaggacac accttggtcg tgccaaaaga
     9841 acactatcgc aatcttttgg agatggatgc tacgagcgcc agccaactct ttgcccaagt
     9901 accaaaagta gctcaaaaag tcatgaaagt cactaaggct gctggtatga atatcatttc
     9961 caactgtgaa gaagtcgctg gtcaaacagt ttttcatact cacgttcacc ttgtacctcg
    10021 ctacagtgct gacgatgacc tcaagattga ttttatcgcc cacgaaccag actttgacaa
    10081 acttgctcaa gtcgctgaaa ccatcaaaaa cgcttaagga gttgctatga aattatccaa
    10141 cctactgcta tttgcaggag ctgcagccgg aagttatctg gtcacaaaaa atcgccaaac
    10201 catcacagat gaagtcttga ataccactga ccgcgttcaa gctatcaagg acgatgtgga
    10261 tattatccaa aacagcctgc aaatcattga ccagcaaaaa gaacttatca aggaatacca
    10321 agaagacttg acttacaagt ttaaggtctt ggaaaaggat atccagacta gactagctgt
    10381 gataaaagaa atgcagggaa ctgaagataa gtaaaaagag cccgacggct cttttttaaa
    10441 acgtaaacaa gttatagtga attgaattta gaatagcaca tcatagtttc taaagcattg
    10501 ttagagatta ctttaaaatc ctctt
//
LOCUS       DAVKLW010000069        10266 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKLW010000069 DAVKLW010000000
VERSION     DAVKLW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.069"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..389
                     /locus_tag="VW324_001796"
                     /pseudo
     CDS             1..389
                     /locus_tag="VW324_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            475..687
                     /locus_tag="VW324_001797"
                     /pseudo
     CDS             475..687
                     /locus_tag="VW324_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            587..1033
                     /locus_tag="VW324_001798"
     CDS             587..1033
                     /locus_tag="VW324_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9727371.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VW324_001799"
     CDS             complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VW324_001799"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9727372.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2639..3085
                     /locus_tag="VW324_001800"
     CDS             2639..3085
                     /locus_tag="VW324_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9727373.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3082..3885)
                     /locus_tag="VW324_001801"
     CDS             complement(3082..3885)
                     /locus_tag="VW324_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9727374.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQNFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3894..4223)
                     /locus_tag="VW324_001802"
     CDS             complement(3894..4223)
                     /locus_tag="VW324_001802"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9727375.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4168..4539)
                     /locus_tag="VW324_001803"
     CDS             complement(4168..4539)
                     /locus_tag="VW324_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9727376.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VW324_001804"
     CDS             complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VW324_001804"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9727377.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VW324_001805"
     CDS             complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VW324_001805"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9727378.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7157..8299)
                     /locus_tag="VW324_001806"
     CDS             complement(7157..8299)
                     /locus_tag="VW324_001806"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9727379.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTIKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VW324_001807"
     CDS             complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VW324_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9727380.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3136 a         2264 c         1815 g         3051 t
ORIGIN      
        1 caccaagtaa aaggaacaaa accaagaaaa gttgatagag atagacttaa aaactatctt
       61 actgacaatc cagacgctta tttgactgaa atagcttctg aatttggctg tcatccaact
      121 accatccact atgcgctcaa agctatgggc tacactcgaa aaaaaagaac cacacctact
      181 atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt ttaaagcacc
      241 tagcacaggt ctaacaaatg aatggtgagt taatcgctcc aatgacttac gaagagacga
      301 tgacgagcga cttttttgaa gcttggtttc agaaatttct cttaccaaca ttaaccgcac
      361 cattggttat tatattaaaa cgaagtaaga ataggacaaa tcgatcagga cagtaaaatc
      421 taattctaac aatgttttag aagtagaggt gtactattct agtttcaatc tactatatta
      481 atggacaatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
      541 cataaacttt tacctcttcc tccctactcg cctgagtaca atcttattga gaaaacatgg
      601 gctcatatca aaaagcacct caaaagggta ttaccaagtt gcaatacctt ttatgaggct
      661 cttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      721 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaccag ttcatagttc
      781 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      841 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      901 tttaaaaaca atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta
      961 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
     1021 aaagattttt taaaaagttt tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg
     1081 gataaaatcc gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg
     1141 gttcgggata aaatagttca ctgaactatt ttatttttta aggttgtaga atgatttcaa
     1201 tccacgatat tcagctactt caccaagttg gtcttcgata cgaagcaatt ggttgtattt
     1261 agcgatgcgg tctgtacgtg aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac
     1321 tgcaatatca gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caacagcagt
     1381 gtaaccagct tctttagcca tttcgatagc ttcaaaagtt tcagtaagag taccgatttg
     1441 gttaactttg ataaggattg agttagcagc accttcttgg ataccacgtg caaggtagtc
     1501 agtgtttgtt acgaagaagt cgtcaccaac aagttgtact ttcttaccaa gacgttcagt
     1561 aagagctttc caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg
     1621 gtatttgtta accaattctt caaggtagtc gatttgttct gcagatgtac gaacagcagc
     1681 accttcacct tcaaatttag tgtagtcgta aactttacgt tctttatcgt agaattctga
     1741 tgaagcacag tcaaatccga taaatacgtc tttacctggt acatatccag cagcttcaat
     1801 cgcagcaagg atagtttcaa caccatcttc agttccttcg aaacgaggag cgaatccacc
     1861 ttcgtcacct acggcagttt ccaaaccacg tgatttaagg attttcttaa gagcgtggaa
     1921 gatttcagca ccgtaacgaa gggcttcttt aaatgttggc gcaccaactg gcaagatcat
     1981 gaactcttgg aaagcgattg gagcgtcaga gtgagaacca ccgttgatga tgttcatcat
     2041 tggagttgga agaactttag tgttgaatcc accaaggtag ctgtaaagtg ggatttcaag
     2101 gtagtcagca gcagcacgag ctacagcgat agacacaccg aggattgcat tcgcacccaa
     2161 tttacctttg ttaggagtac cgtcaagtgc aatcatagca cggtcaatag cttgttgatc
     2221 acgtacatcg tagccaatga tagcttcagc aatgatgttg tttacgttgt caacagcttt
     2281 ttgtgtacca agaccaccgt aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg
     2341 ttcaccagta gaagctcctg atggaaccat accacgtccg aaagcacctg attcagtgta
     2401 aacttctact tcaagtgttg ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc
     2461 agtaataatt gacatttttt actctcctta tgagttaaat tttttacacc tctataatac
     2521 cttaaaaccc ctcctttttc aagaaaaaac gttatctttg tgcaactttt ccttaacttt
     2581 ataaagtaat cgctttcttt tgtctgtttt attctaactt ttatgatata ctgttttcat
     2641 gacagattta tcaaaacaat tacttgaaaa agctcatggt gggttaaaaa taaatccgga
     2701 tgagcaaaga cgctatcttg gtacttttga ggaaagagtt cttggatatg tagatattga
     2761 cacagcaaat agccctcagt tagaaaaagg ctttttattt attttagaaa accttcagga
     2821 aaaagcaaag ccactatttg tgaagatttc accaactatc gaatttgata agcaagtttt
     2881 ctacttaaaa gaagcaaaag aaactgatag tcaagccacc atagtatctg aagagcatat
     2941 tacttctcct tttggcctgg ttattcatag caatgcacca gttcaagtag aagaaaaaga
     3001 ccttcgactt gcttttccaa aactttggga agttaaaaag gaagaaccag ccaaaacatc
     3061 cttatggaag aaatggttta gctaaatctt gcacatattt aataagtgcc caatattggc
     3121 agccgtgcgc tccagataga gactggcatt tttcaaacta tcttctaaag gttcactttt
     3181 ctccaaaata gaaaagacag cttggatatt ttcaaatggt aggggaggta aatcttcagc
     3241 aagactaccg caaatagcaa taacaggaac tccaacaggg gttctttttg caacacctat
     3301 aggcgctttc ccagcaaagt tttgactatc aagtcttcct tctccaacaa caaccaagtc
     3361 agcatctgaa actttcttat caaagttgat taagtccaag caggtatcaa ttccagacac
     3421 gatacttgcc tgagcaaagg cacacaaacc agcagcaatc cccagtcctc catcattact
     3481 ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagaaatga gatggagaat
     3541 caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta gcggaatttt
     3601 tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt gaaaatagcg
     3661 catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt ttaggtcaag
     3721 agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc agaggagaca
     3781 ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta cctgttgagc
     3841 tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt ccctcattct
     3901 aaacagtcaa tcaaaggaag aacttctaaa aaatccctct tgtcaacatg atgtggtatt
     3961 tcttttttga gcatttcttt ggaacaaaag gcgattccta actccgccga cttcaacatt
     4021 aatagattat taaccccatc accgattgcc accgttcttt ctttagaaag ttttagtttc
     4081 tttctccatt tttccagagt ttcttttttg acctggggac ttataatttg tccaactaat
     4141 tttcctgtta aaagaccttc tttgacttca agctggttgg cagtgaaata ggcaatacca
     4201 agggattttg ctaatctctc caactattgg tgtaaatcca ccagacacca gaccaactag
     4261 gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata gatgaattga
     4321 gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca ctctttttct
     4381 taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct gcgaaatttc
     4441 cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga ttaaggttcc
     4501 atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc tctaaacagc
     4561 ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa ctatggtaca
     4621 agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata gcttgttttt
     4681 gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt tggaaagtcc
     4741 aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt ccttcgattg
     4801 gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat ggcaaggttc
     4861 cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt ggcatgagga
     4921 agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg atatttgctg
     4981 atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag gctggatcgc
     5041 cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga ctttcgacca
     5101 ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt ggaacgtctg
     5161 ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct ttcccagaca
     5221 aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta taaagatcag
     5281 catcaatccc attcacgata ccagatactt taccagactc cattttaaga atctgatcca
     5341 aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa acggttgaaa
     5401 cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc catcgaaggg
     5461 tgccatcagc gtaacgttca aggccaactc caaacaaatc acccaacatt ccttctgaaa
     5521 attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgtcc tcataggctt
     5581 gaatccaaca gtatttttcc ttcaacaaga aaggaatcat agctgtatgg tagtcatgaa
     5641 catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca gccagttgga
     5701 aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg aagaaataat
     5761 attgattgtc aataaagtag aaggttacac catttaatac tgttttctta attccacaat
     5821 actgtctacg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc tgatttccaa
     5881 atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc ccagctttta
     5941 ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt gaaaagggtg
     6001 caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta ctttagcacc
     6061 tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccaa gctcaacttc
     6121 aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa caacacgagg
     6181 gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac gtgatagaac
     6241 agaattagct acttgacctt caataatact accagaggca aactgagaag tgcttacctt
     6301 agatgtatta gcataataag ttggctcttc gtttttgacc tttgtataaa tcttttggtt
     6361 tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatatttg cttgataata
     6421 agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcaa aagctccctc
     6481 ttttacagcc aaatcccgta aaacatagcg caatttctct ggatgttctt ttttagcttc
     6541 ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg tagacatatt
     6601 gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat ggtctgtttc
     6661 atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt ttttataaac
     6721 tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca aatcaatgtt
     6781 aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt tcaaataagt
     6841 aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg tattgtaaat
     6901 tcctagatag taatggctaa gaagggttga taagccccac tcgcgccctg aacgaatatg
     6961 gtcaaagact gagctgatat tatcctgctg gaaaatacca aagacactac gaacacctgc
     7021 attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa atggcaaact
     7081 tgctactgga cggtggtccg tcaatgtcga catatcgtga aaaccaactg tatttcctaa
     7141 aatggcagaa tatttatcaa tcttcatctg ttgctacccc cactacttca ttatatccta
     7201 caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc gcaccttcac
     7261 caataatggc acgtttaatc ttagctcctt gaccaataat agctccactc atgataactg
     7321 aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata gaatgtttaa
     7381 tagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttccacatga gcatttgccc
     7441 cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga cggttacgac
     7501 tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt gactcaatag
     7561 taccaacatc tttccaataa ccactaaatt cgtaagcata aacactttca cctgactcaa
     7621 ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc ttttcagcag
     7681 cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata gaagcttttg
     7741 tagatttagg ttgagctggt ttttcttcaa attcaacaat acgattgtta gcatctgtgt
     7801 tcatgatacc aaaacggctc gcttctttaa gagggacgtc taaaactgct actgtcaagc
     7861 tggcattatt atccttatga gactggagca tatcatcata gtccattttg tagatgtggt
     7921 ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg atattttggt
     7981 aaatagcgtg actagttccc tcaaaccaac gatttccttc acttgcagaa taaggttgaa
     8041 gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc ccaatatggt
     8101 tgttgagagc aagtggttga tactgtgtaa ccaccccaac attatgaatc cctgagttgg
     8161 cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc acagctggtt
     8221 ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca agaatcaaag
     8281 ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac ggttttagta
     8341 gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag ggtaaaggtt
     8401 aaggtctgct cataatcttt ccataggcct tcttgcgttt gaacagtttg attatgttct
     8461 ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta aattcctgca
     8521 acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatgttaaa gatacagact
     8581 aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc tcgattatcc
     8641 gcatcaatga tttcaatacc atcatagctg gtatcgattt cccacagaca gcgatgatct
     8701 ttgtaaaact ggtttagctg agaagtgaaa tacttcatct tagcattcat tgggtcttct
     8761 aggttagacc attccaactg ttcttcagat ttccattcta ggaattgacc gtattcgcta
     8821 cccatgaaga gcaatttctt accagggtga caaatttggt acgtatagag attgcgcaag
     8881 cctgcgaatt gattgtaacg atctccccac atcttatgca tcatactctt cttgccatga
     8941 accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata cataaagctg
     9001 aaagtcacca ggttaaagtc atatttacga taaatcggat cttcttcgta gaaacggagg
     9061 atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc aatctctttc
     9121 attcccgtaa tcttggtcgc agacgaactt tcttctgcaa tcatcatcac atctggatat
     9181 tctaacttaa taacttcatt caagcgttga agaaaataat aaccttcata gttgagattt
     9241 ccgccatctt tattaggtgt ccatggagca ttatcatagt ccaaatagag catgttgcta
     9301 acagcatcca cacgaatacc atccaaatga tagacatcaa tccaatgctt aatgcaagaa
     9361 attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc ccaaccatgg
     9421 ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata ataggctaag
     9481 gcatcatcgt tgatggtaaa gtgacctggt acccagtcca caataacccc aatattatgg
     9541 gtatgacact cctcgacaaa atcttgaaac tcctctggtc tgccataagc atgctctaaa
     9601 gcgaagtaac ccataagctg atacccccaa ctcaagccca aaggatggga catcaagggc
     9661 ataaactcaa tatgagtata gttcatttca acgagataag gaatgagttc atccttgagc
     9721 tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc gtgaacttca
     9781 taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag ccaaagtcca
     9841 tccttccatt tcttctcagg aagctctgtt aagattgccc ctgttcctgg acgagcctca
     9901 tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg tgtgacatga
     9961 tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac cccaaaatca
    10021 tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac caagtgaaca
    10081 gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt tagttcttcc
    10141 ctatgtgctc ctagataatg ttggagataa aaattttcac ccgtcataaa ggtttttaat
    10201 gcttctctat tatccatata ctccccttct cctgtaagcg ttttctatgt ttttattata
    10261 ctacct
//
LOCUS       DAVKLW010000070        10185 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKLW010000070 DAVKLW010000000
VERSION     DAVKLW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.070"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            256..603
                     /gene="ssrA"
                     /locus_tag="VW324_001808"
     tmRNA           256..603
                     /gene="ssrA"
                     /locus_tag="VW324_001808"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(786..873)
                     /locus_tag="VW324_001809"
     tRNA            complement(786..873)
                     /locus_tag="VW324_001809"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Ser,seq:gga)
     gene            1035..1775
                     /locus_tag="VW324_001810"
     CDS             1035..1775
                     /locus_tag="VW324_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9727381.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1772..2683
                     /gene="pfkB"
                     /locus_tag="VW324_001811"
     CDS             1772..2683
                     /gene="pfkB"
                     /locus_tag="VW324_001811"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9727382.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2680..4632
                     /locus_tag="VW324_001812"
     CDS             2680..4632
                     /locus_tag="VW324_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9727383.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4796..7021
                     /locus_tag="VW324_001813"
     CDS             4796..7021
                     /locus_tag="VW324_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9727384.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7174..7602)
                     /locus_tag="VW324_001814"
     CDS             complement(7174..7602)
                     /locus_tag="VW324_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727385.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            7740..8882
                     /locus_tag="VW324_001815"
     CDS             7740..8882
                     /locus_tag="VW324_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9727386.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8891..10105
                     /gene="thiI"
                     /locus_tag="VW324_001816"
     CDS             8891..10105
                     /gene="thiI"
                     /locus_tag="VW324_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9727387.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
BASE COUNT         2944 a         1957 c         2314 g         2970 t
ORIGIN      
        1 attcgttcta ttcttatttc attttactat attttggtgc aataagtgaa aagtagaccg
       61 aataatataa ggattgattt tatagttttt aaactcaaat gaattgaaat aaagagagta
      121 cgaaaattct catctgaaag tattttagaa taattctctt cgtgaatttc ttcaaaacag
      181 atagcttcat cttaggtatg tgatttcttt ttgcattttt gagttagata aggtataatg
      241 gttttattgt cttttggggt cgttacggat tcgacaggca ttatgaggca tattttgcga
      301 ctcgtgtggc gacgtaaacg ctcagttaaa tataactgca aaaaataaca cttcttacgc
      361 tctagctgcc taaaaaccag caggcgtgac ccgatttgga ttgctcgtgt tcaatgacag
      421 gtcttattat tagcgagata cgattaagcc ttgtctagcg gcttgataag agattgatag
      481 actcgcagtt tctagacttg agttatgtgt cgaggggctg ttaaaataat acataaccta
      541 tggttgtaga caaatatgtt ggcaggtgtt tggacgtggg ttcgactccc accggctcca
      601 ttattccttt gcattctttt gcattccttg gtaaaacgtt gttaaatcaa cgttttttat
      661 ttttatcttt ggtattcctt tgtattcttt tgctaaaaag ggagtcacaa acagacccta
      721 ttttaaaaaa aggatagaaa aaaggataca acatttgtcg catcctaaaa ataatctttt
      781 ttcgacggaa gacatgggat tcgaacccac gcacgctatt acacgcctac cgcgtttcca
      841 acacggcctc ttaagcctct tgagtaatct tccaatactt actcaaatag tctaccataa
      901 aggctcttat cttgcaataa aaattctaga aataagaaaa atgatagatt ttgaaagaaa
      961 atgataaaaa atgcttgact tcgaaagaaa gtatgataga atgaatagtg taaacgataa
     1021 caggaggtga ttcagtgtta aaaacagaac gtaaacaact aattttagag gagttaaatc
     1081 agcatcatgt agtttctcta gaaaaattag ttagtttgct agaaacgtca gaatcaacgg
     1141 ttcgaagaga cttggatgag ttggaagcgg aaaacaagct tcgtcgtgtg catggtggag
     1201 cagaactccc ccactcctta caggaagaag aaaccattca agaaaaatct gtcaaaaacc
     1261 ttcaagaaaa gaagttgctg gctcagaaag cagcctctct cattaaagaa aaagatgtca
     1321 tctttatcga tgctggaaca acaactgctt ttttgattca tgaattggtc aataagaatg
     1381 ttacagttgt gaccaactcc attcaccatg ccgctcagtt ggttgaaaag cagattccaa
     1441 ctgtcatggt tggaggaaac gtcaagacgg cgacagatgc tagtatcggg ggcgttgctc
     1501 ttaaccagat taaccaattg cactttgacc gtgcctttat cggaatgaat ggtgttgacg
     1561 atggctatta tacgactcct gatatggagg agggagctgt gaaaagagct attttggaga
     1621 atgccaagca gacctacgtc ttggtggatt cgtcaaaaat tggacaaact tgctttgcca
     1681 aggtagcacc actcaaacgc gctatcgtta tcactagtca agggcatgag ctcttgcagg
     1741 ttattaagga gaaaacggag gtaatagaag tatgatttat acagtcacac tcaatccatc
     1801 cattgactat atcgttcgtt tggaccaagt caaagttggt agtgtaaatc gtatggacag
     1861 tgatgataag tttgctggtg ggaaaggaat caatgtcagc cgtgtcttga aacgtttgaa
     1921 tataccaaat acagcgacgg gatttatcgg tggctttact ggtaaattta tcacagatac
     1981 tttagcagag gaagaaatcg agacacgttt tgtccaggtg gcagaagata ctcgtatcaa
     2041 tgttaaaatc aaagcagacc aagaaacaga aatcaacgga acgggtccaa ctgttgaacc
     2101 ggttcagcta gaagaattga aagctatttt atctagtctg acagcagaag atacagttgt
     2161 ctttgcaggt tcaagtgcta aaaatctagg caatgttatc tataaggatt tgatttcctt
     2221 gacgcgccag actggtgcgc aagtggtctg tgactttgaa ggacagacct taattgatag
     2281 tttggactac cagcctcttc ttgtaaaacc aaacaatcat gaacttggag cgatttttgg
     2341 ggttaaactc gaaagtttag atgaaattga gaaatacgct cgtgagttac tggctaaggg
     2401 tgctcaaaat gttattatct ctatggctgg tgatggcgcc cttcttgtca catctgaggg
     2461 agcttacttc gctaaaccaa tcaaaggaac agtcaaaaat tcagttggag ctggtgattc
     2521 tatggttgct ggattcacag gtgaatttgt caaatcaaaa gacgcagtag aagccttcaa
     2581 atggggagtg gcttgcggaa cggcaactac cttctcagat gacttggcaa cggcggaatt
     2641 tattaaagaa acatatggaa aagttgaggt agaaaaaaga tgaaaattca agacctattg
     2701 agaaaagatg tcatgttgct agatttgcag gcaactgaaa aaacagctgt catcgacgag
     2761 atgattaaaa atttgacaga gcacggttat gtaacagatt ttgaaacatt taaagaagga
     2821 attttggcgc gtgaagcttt gacttctact ggtttgggtg atggaatcgc aatgcctcac
     2881 agcaaaaacg ctgctgtcaa agaagcgaca gttctatttg ctaagtcaaa taagggtgtt
     2941 gactacgaga gcttggatgg acaagcaact gacctcttct tcatgattgc agctccagaa
     3001 ggtgccaatg atactcactt ggcagccttg gcagaattgt ctcaatactt gatgaaagac
     3061 ggttttgcag acaaacttcg tcaagcaaca tctgcagacc aagttatcga actttttgac
     3121 caagcttcag aaaaaactga ggaacttgtt caagcacctg ctaatgactc tggtgacttt
     3181 atcgtagctg ttacagcttg tacaacaggt attgcccaca cttacatggc ccaagaagcc
     3241 cttcaaaaag tagctgctga aatgggggtt ggtatcaagg tcgaaaccaa cggtgctagc
     3301 ggtgttggaa atcaactaac tgcagaagat atccgtaagg ctaaagctat tatcattgca
     3361 gcagacaagg ccgttgaaat ggatcgattt gatggaaaac cattgatcaa tcgtccagtt
     3421 gctgacggta tccgtaagac agaagagcta attaacttgg ctctttcagg agatactgaa
     3481 gtctaccgtg ccgctaatgg tgccaaagct gcaacagcct ctaacgaaaa acaaagcctt
     3541 ggtggtgcct tgtacaaaca cttgatgagt ggtgtatctc aaatgttacc attcgttatc
     3601 ggtggtggta tcatgattgc ccttgccttc ttgattgacg gtgctttggg tgttccaaat
     3661 gaaaaccttg gcaatcttgg ttcttaccat gagttagctt ctatgttcat gaaaattggt
     3721 ggagctgcct ttggtttgat gcttccagtc tttgcgggtt atgttgccta ctctattgct
     3781 gaaaaaccgg gtttggtagc aggtttcgtg gctggtgcta ttgccaaaga aggttttgcc
     3841 tttggtaaaa ttccttatgc cgcaggtggt gaagcaactt caactcttgc aggtgtctca
     3901 tctggtttcc taggtgccct tgttggtgga tttatcgcag gtgccttggt tcttgccatc
     3961 aagaaatacg ttaaagttcc tcgttcactc gaaggtgcta aatcaatcct tctattgcca
     4021 cttcttggaa caatcttgac aggatttgtt atgctagctg tgaatatccc aatggctgca
     4081 atcaacactg ctatgaatga cttcctaggc ggtcttggag gaggttcagc tgtccttctt
     4141 ggtatcgtcc ttggtggaat gatggctgtt gacatgggtg gaccagttaa taaagcagct
     4201 tatgtctttg gtacaggtac gcttgcagca actgtttctt caggtggttc tgtagccatg
     4261 gcagcagtta tggctggagg aatggtgcca ccacttgcaa tctttgtcgc aactcttctt
     4321 ttcaaagata aatttactaa ggaagaacgt aactctggtt tgacaaacat catcatgggc
     4381 ttgtcattta tcactgaggg agcgattcca tttggtgccg ctgacccagc tcgtgcgatt
     4441 ccaagcttca tccttggttc agcagtagca ggtggactcg ttggtcttac tggtatcaaa
     4501 ctcatggcgc cacacggagg aatcttcgtt atcgccctta cttcaaatgc tctcctttac
     4561 ctcgtttctg tcttggtagg agcaatcgta agtggtgtgg tttatggtta cctacgcaaa
     4621 ccacaagcat aaaaaataga aaaatgaaaa gattggaccg tttggtgcag tctttttctc
     4681 ttcccgaaat gcctgtgaaa tatggtataa tagaagaatg gcaaacaaga atacaagtac
     4741 aacaagacgg agaccgtcta aagcagaact ggaaagaaaa gaagcgattc aacgaatgtt
     4801 gatttcgtta ggaattgcga ttttattgat tttcgcagcc ttcaaattag gggctgcagg
     4861 tataaccctt tataatttaa ttcgcttgct agtgggtagc ctagcttatc tggcgatatt
     4921 cggcctatta atctatctct tctttttcaa gtggatacga aaacaggaag gactcttatc
     4981 tggctttttc accatatttg ctggcttact cttgattttt gaggcctact tggtttggaa
     5041 atatggtttg gacaagtccg ttctaaaagg gaccatggct caggttgtga cagatctgac
     5101 tggttttcga acgactagct ttgctggagg gggcttgatc ggggtcgctc tttatattcc
     5161 aacagccttt ctcttttcaa atatcggaac ttactttatt ggttctatct tgattttagt
     5221 gggttctctc ctagtcagcc cttggtctgt ttacgatatt gctgaatttt tcagtagagg
     5281 ctttgccaaa tggtgggaag ggcacgagcg tcgaaaagag gaacgctttg tcaaacaaga
     5341 agaaaaagct cgccaaaagg ctgagaaaga ggctagatta gaacaagaag agactgaaaa
     5401 agccttactc gatttgcctc ctgttgatat ggaaacgggt gaaattctga cagaggaagc
     5461 tgttcaaaat cttccaccta ttccagaaga aaagtgggtg gaaccagaaa tcatcctgcc
     5521 tcaagctgaa cttaaattcc ctgaacagga agatgactca gatgacgaag atgttcaggt
     5581 cgatttttca gccaaagaag cccttgaata caaacttcca agcttacaac tctttgcacc
     5641 agataaacca aaagatcagt ctaaagagaa gaaaattgtc agagaaaata tcaaaatctt
     5701 agaagcaacc tttgctagct ttggtattaa ggtaacagtt gaacgggccg aaattgggcc
     5761 atcagtgacc aagtatgaag tcaagccggc tgttggtgta agggtcaacc gcatttccaa
     5821 tctatcagat gacctcgctc tagccttggc tgccaaagat gtccggattg aagcaccaat
     5881 ccctgggaaa tccctaatcg gaattgaagt gcccaactcc gatattgcca ctgtatcttt
     5941 ccgagaacta tgggaacaat cgcaaacgaa agcagaaaat ttcttggaaa ttcctttagg
     6001 gaaggctgtt aatggaaccg caagagcttt tgacctttct aaaatgcccc acttgctagt
     6061 tgcaggttca acgggttcag ggaagtcagt agcagttaac ggcattattg ctagcattct
     6121 catgaaggcg agaccagatc aagttaaatt tatgatggtc gatcccaaga tggttgagtt
     6181 atctgtttac aatgatattc cccacctctt gattccagtc gtgaccaatc cacgcaaagc
     6241 cagcaaggct ctgcaaaagg ttgtggatga aatggaaaac cgttatgaac tctttgccaa
     6301 ggtgggagtt cggaatattg caggttttaa tgccaaggta gaagagttca attcccagtc
     6361 tgagtacaag caaattccgc taccattcat tgtcgtgatt gtggatgagt tggctgacct
     6421 catgatggtg gccagcaagg aagtggaaga tgctatcatc cgtcttgggc agaaggcgcg
     6481 tgctgcaggt atccacatga ttcttgcaac tcagcgtcca tctgttgatg tcatctctgg
     6541 tttgattaag gccaatgttc catctcgtgt agcatttgcg gtttcatcag gaacagactc
     6601 ccgtacgatt ttggatgaaa atggagcaga aaaacttctt ggtcgaggag acatgctctt
     6661 taaaccgatt gatgaaaatc atccagttcg tctccaaggc tcctttatct cggatgacga
     6721 tgttgagcgc attgtgaact tcatcaaggc tcaggcagat gcagactacg atgagagttt
     6781 tgatccaggt gaggtttctg aaaatgaagg agaattttcg gatggagatg ctggtggtga
     6841 tccgcttttt gaagaagcta agtctttggt tatcgaaaca cagaaagcca gtgcgtctat
     6901 gattcagcgt cgtttatcag ttggatttaa ccgtgcgacc cgtctcatgg aagaactgga
     6961 gatagcaggt gtcatcggtc cagctgaagg taccaaacct cgaaaagtgt tacaacaata
     7021 aaaaaatagc ttctttccga gtttggaggg aagctatttt agtggctatt gattactttt
     7081 atactcttcg caaatctctt caaaccacgt cagcttcgcc ttgccgtact caagtactgt
     7141 ctacggctag cttcctagtt tgctctttga ttttcattga gtattatttt ctgaagttgg
     7201 agtattgggc tgtttttcat tttcagtggc aggtttactt gtagcaggtg tagtagagtc
     7261 ctgagttgct gttttctgat cttctttttt ctcttccttg acgctagatt ttggcgtttc
     7321 ttcttgttgt gttttttctt gagaagtgtt attttcctta tttggagtag tattttcctg
     7381 aggggattct ttttgaattt ctttgacaaa ggttgtcgtc tggcttgtcg taggttcttt
     7441 tttagtattt ttattattat tatccaaggc gttcatgatc gtgatacccg cggtaatcaa
     7501 accaaggata ctaccgatag tagtaatggt tttttgaaaa acagtaaagc cttttttgat
     7561 atgttctgtt ttcggttttg aagttctacg atagttagac atacattctc tcctttgatt
     7621 atgatttatt atactctgtt tctttaaaaa aatcttaaaa aaagagaaat tgccctttct
     7681 tgtcgcaggc tccgtttcat gatacaatag aaggagtgat tttagaaagc gtgagaaaca
     7741 tgatttactt tgataattcg gcgacgacca ggccctatcc agaagccctt gaaacctata
     7801 tgcaggtggc ttcaaaaatt ttaggaaatc catctagtct ccatcgtttg ggagaccagg
     7861 caacacgaat tttagatgct tcccgtcaac agattgcaga tttaatcggt aagaaaagtg
     7921 atgaaatctt ctttacatct ggtgggacag aaggggataa ctggatcatc aagggagtag
     7981 catttgaaaa agcccagttt ggcaagcaca tcatcgtatc agccattgaa catccagcag
     8041 ttaaggaatc agccctctgg ttgaaagctc aaggttttga agtggatttt gctccagttg
     8101 ataacaaagg atttgtggat gttgaggcgc tagaagattt gataagacct gatacgaccc
     8161 tcgtttccat catggctgtg aacaatgaaa tcggctctat ccaacctatt gaggctattt
     8221 cagaattctt ggcagacaag ccaactattt ccttccacgt tgatgcggtt caggcgcttg
     8281 ccaagattcc gactgaaaag tatctgacag aacgagtgga tttcgcgact ttctctggtc
     8341 acaagttcca cggaatccgt ggtgttggtt ttatctatat caagtctggc aagaagatta
     8401 cgcctctttt aacaggtggt ggtcaggaac gtgattaccg ttcgacaact gaaaatgtgg
     8461 caggaattgc agcgaccgct aaggctctcc gtttatctat ggaaaagcta gatatcttta
     8521 ggagcaagac tgggcagatg aaggcagtga ttcgtcaagc acttctggac tatccggata
     8581 tatttgtctt ttcagatgag gaagacttcg cccctcatat tctgactttt ggaatcaagg
     8641 gtgttcgtgg tgaggtcatt gttcacgcct ttgaagacta tgatattttc atctcaacga
     8701 cctcggcttg ttcgtccaag gctgggaaac cagccggaac cctgattgct atgggagtgg
     8761 acaaggataa ggctcagtca gctgtgcgtc ttagtctaga ccttgaaaat gatatgagtc
     8821 aggtcgagca gttcttgacc aagctaaaat taatttacaa tcaaactaga aaagtaagat
     8881 aggagcattc atgcagtatt cagaaattat gattcgctac ggtgagctgt caaccaaggg
     8941 caaaaatcgt atgcgtttca tcaataaact tcgcaataat atttcagacg ttttgtctat
     9001 ctatccccaa gttaaggtaa cagcagatcg cgaccgtgcc cacgcttacc tcaatggagc
     9061 agattacaca gcagtagcag aatcgctcaa acaagtattt ggaattcaaa acttttcccc
     9121 tgtttataag gttgaaaaat ctgtagaagt tctgaagtct gctgtccaag agattatgca
     9181 ggacatctac aaggaaggca tgacctttaa aatctctagc aagcgtagcg accacacctt
     9241 tgaattggac agtcgtgaac tcaaccaaac gcttggaggg gctgtattcg aagccattcc
     9301 aaacgtacaa gctcaaatga aaagtcctga catcaatctt caggtggaga tacgtgaaga
     9361 agcagcctat ctttcttatg aaaccgttcg aggggcaggt ggattaccag tgggaacttc
     9421 tggtaagggc atgctcatgt tgtcaggggg aatcgattcc cctgtagctg gctatctagc
     9481 gcttaaacga ggggtagata tagaggcagt tcactttgcc agcccacctt acacgagtcc
     9541 tggtgctctt aaaaaagccc aagatttgac ccgtaaatta accaaatttg ggggaaatat
     9601 ccagtttatc gaagttcctt ttacagagat tcaagaggaa atcaaagcca aagcgccaga
     9661 agcctacctt atgaccttga cgcgtcgttt tatgatgcgt attacggacc gtattcgtga
     9721 ggtacgaaat ggtttagtta tcatcaatgg ggaaagtcta ggtcaagtag ccagccaaac
     9781 ccttgaaagt atgaaggcta tcaatgctgt taccaacact cccatcattc gtcctgtggt
     9841 taccatggac aagttggaaa tcattgacat cgcccaggaa atcgatacct ttgacatttc
     9901 aatccaaccg tttgaagact gttgtactat ttttgcacca gatcgtccaa aaacaaatcc
     9961 taaaattaag aatgcggagc agtacgaagc gcgtatggat gttgaaggct tggttgagcg
    10021 agcagtggct ggaatcatga ttactgaaat cacacctcaa gccgaaaaag atgaagttga
    10081 tgacttgatt gacaatctgc tctaattcag aaaatccaaa agaatagcga aaatcagtaa
    10141 aaaaagttag ttttttctct aaaaacaggt aaaaaactaa ctttt
//
LOCUS       DAVKLW010000071         9750 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKLW010000071 DAVKLW010000000
VERSION     DAVKLW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.071"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            25..954
                     /locus_tag="VW324_001817"
     CDS             25..954
                     /locus_tag="VW324_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001150386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9727388.1"
                     /translation="MQDESIYGLVYKTIREERGFTQKEAAGTTVTPHFLRQFEQGKSR
                     ITIQKFEEILSNIGIDKETFDSLKTQMFPTEFHKLQAQVADLAYKREFKKIISLINQP
                     MENPGIAEHYVIANRVVNKFAIANIIGDSFLTPKDYQELEYIKNYLSELDDWNKIEVN
                     IFSSILPHFSIEFLDYRLYHLLDTLKKQTEYHSIRTADYYIACLRTAIKHYSVNGYYD
                     KAESLAVKTLEVINTFPLLSTKMTEMISLSMERANNFLRKDDVRGLELAKHIFASLDN
                     FEKVYPNQLLTRMREDFFVTVTQLNHTGQPLDV"
     gene            complement(1004..3340)
                     /locus_tag="VW324_001818"
     CDS             complement(1004..3340)
                     /locus_tag="VW324_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9727389.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLGPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGAALNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESRVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQVDQD
                     NDVNRIIEQLEEQTLESHKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKILQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQVQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(3444..3992)
                     /locus_tag="VW324_001819"
     CDS             complement(3444..3992)
                     /locus_tag="VW324_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9727390.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(3989..4291)
                     /gene="zapA"
                     /locus_tag="VW324_001820"
     CDS             complement(3989..4291)
                     /gene="zapA"
                     /locus_tag="VW324_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9727391.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            4378..5259
                     /locus_tag="VW324_001821"
     CDS             4378..5259
                     /locus_tag="VW324_001821"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9727392.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVITRDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5263..5877
                     /gene="lepB"
                     /locus_tag="VW324_001822"
     CDS             5263..5877
                     /gene="lepB"
                     /locus_tag="VW324_001822"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9727393.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            6005..8371
                     /locus_tag="VW324_001823"
     CDS             6005..8371
                     /locus_tag="VW324_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9727394.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(8419..9702)
                     /locus_tag="VW324_001824"
     CDS             complement(8419..9702)
                     /locus_tag="VW324_001824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9727395.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         2753 a         2183 c         1983 g         2831 t
ORIGIN      
        1 aaaaaggatt cgaggagatt taggatgcaa gatgaaagta tttatggatt agtctataag
       61 accatccgtg aggaaagagg gtttacacag aaggaagctg ctgggactac agtgactcct
      121 cattttttac gtcagtttga gcaagggaaa tctcgaataa ccattcaaaa atttgaagag
      181 attctttcta atattggaat tgataaagag acctttgata gtctcaaaac acagatgttt
      241 ccaacggaat ttcataaatt acaggctcaa gttgctgatt tggcctataa gcgtgaattt
      301 aaaaagatca ttagtttgat taatcaaccc atggaaaatc ctgggatcgc tgaacactat
      361 gtgattgcca atcgtgtagt caataaattt gctatcgcaa acatcatcgg cgattctttt
      421 ctgactccaa aagattacca agaactagaa tatatcaaaa actatctgag tgaattagac
      481 gactggaaca aaattgaggt caatatcttt tcatctatcc tgcctcactt ttcaatcgaa
      541 tttttagatt ataggctcta ccacttatta gatactctaa aaaaacagac tgaataccat
      601 tcaatccgta ctgctgacta ctatattgcc tgcttaagaa ctgctatcaa gcattactct
      661 gttaatggct actatgataa agctgagagc ttagctgtga aaaccttgga agtgattaat
      721 actttccctc tactgagcac caagatgaca gagatgatta gtctttccat ggaacgggct
      781 aataattttc tcagaaaaga tgatgtcaga ggtctagagc ttgctaaaca tatctttgcc
      841 agtcttgata actttgaaaa ggtctatcca aatcaacttc tgactcgaat gagagaggat
      901 ttctttgtta ctgtcacaca actcaatcac actggacaac ctttagatgt ctaacctaga
      961 aaataaaaaa gcttgaggaa ccacatattt cctcaggctt ttcttatcct ttaaaagtga
     1021 caatagtcgc gccactgcct ccagcatttt gtggggcata gccaaaactc ttgacatgtt
     1081 tgtttctttg taggtatttg gtgactcctt cacggatgac acctgttccg ataccatgaa
     1141 tgatatcaac ttgagccata ttgttaagca gggcttggtc gataaaggca tctagctcat
     1201 tcatggcctc ttcatagcgt ttgcctcgaa ggtccagtct ggcttgtgga cctcggccag
     1261 atgtccgttt aacaacattg acttgtttct tcttaactgg cttttcttgc tgaacttgaa
     1321 caaggtcaaa ctctttctct tccagggtca tcttaatcaa gccaacttgg gcttcccagc
     1381 ggccgtcctt gagttgactg gtcagggtac cacgctggcc ataactgaga accacgatat
     1441 catctcccac ctttggagct cgttttttct tggccttttg aaggatcttg tttttagaca
     1501 aatctacttt ttcaggagcc aattttttca gcttggcctt ggcttcaatg atttcgtggg
     1561 gcttgagttg agatttactg tggagatttt tgagaatctg gtcactctca ctgagggcca
     1621 tgtccacaat ctcagcagcc tgttcacgcg ccttattaag ctcggtttcc ttttcacgat
     1681 tgagttcgtt gtaaagtttt ttgagagcac ggttcatctt gagattttct tgctccacct
     1741 cacggatatt gtccaagcgt ttatgacttt caagcgtctg ctcttccaat tgttcaataa
     1801 tccgattgac atcattgtcc tgatcgacct gctggctggc atcccctacg ataacttcag
     1861 ataggcctag acgtttggca atttcaaagg cattacttcg gccaggaaca ccctgcataa
     1921 agcgataagt cgggcgaaga gttgcagtat caaactccat actagcattt tgcacaaagg
     1981 ctgtctcaat accgtaagcc tttagttccg gatagtgggt agtcgccatg gtcttgattt
     2041 gacgcaggcg aaggtcctcc agaatagcca tggcaagagc agctccttct tgagggtctg
     2101 taccagcccc caactcatcc aagagtaaga gtgaatgttg attgaccttg ccaagaatat
     2161 ccacgatatt ggtcatgtgg ctagagaagg tagacaagct ctgctcaata gactgctcat
     2221 ctccaatatc ggcaaagatt tcttcaaaga taccaacacg actgccctta tctgctaaaa
     2281 tcggcaaacc ggactgagcc atgacctgtg tcaagccgag agttttaagc atgatggtct
     2341 taccacctgt attgggacct gtaatgacaa tagctgttaa atcttgacca aaatggacat
     2401 catttgcgac agcatttttg accaaaggat ggcagacatg gagcagttga atctcttgat
     2461 tttctgacag ctgaggtacg actgcttgcc tttcttggat aaagcggacc ttggcacgaa
     2521 tcaagtctag atgaccgata atccaggcgt cattggcaat ctcagccgca tgagggcgga
     2581 cacgctcaga aatttcttgg agaatgcgaa gcatttcata gcgctcatct gctcgcagac
     2641 tagcaatttc ttcgctcagt ttgactacct cacgtggttc gatatagacg gtgtttccac
     2701 tagcagaaat atcatgaacg acacctgcaa tcttattgcg gtaggtgttt ttgactggta
     2761 aaacctgacg gccatttctg ctagcaacaa ttccttctgt caacatctgc gctttttgct
     2821 tgagcaagtc ttgtaaaaca tcgcgtaccc gactctcgct atcatgtatt tttcgacgga
     2881 ttcgcgccaa ttcttcactg gcaaaatttt caatgaaacc cgcatcatta aaggcctgaa
     2941 gatttccttg taattgcgga aaatcatgta atttctcaaa ccaaagggct aattcttcca
     3001 agctgacatt ttccagattg gcgtaaaaac tttgaagttc tcggctggca agaagcacgc
     3061 gtttcaagag taggaactcc tcgatattga gagccgctcc catctccaac ctcttgcaga
     3121 ctcctgcaat ttccttagtt gagagaatag taaaatgcgg ttgctcgacg aaaagagcct
     3181 gcatttcctt catctcagca aaagcctgtt tgattttatc tgctttggca gtcggaccca
     3241 gctgtctcaa ttgctccaag ccctgctcgg tcaacaaatg aggctcaaac aaggccttga
     3301 ccttatcgaa ctctaatgtt tctaatattt tcttattcat ctttatttcc ttgtctttga
     3361 atgtctaggt gtggtagctt tttacattct gatagattcg agtcaaatct gtaaacaaag
     3421 ggctaggaaa actcctgccc tttttatccg attaaatttg tcacccagat ttgtttgagc
     3481 caactggttg ttatcggtat gctctggatg atgtgttttg cgacgatact cttttcaaga
     3541 ggattttgta taactgccat ggggatggtc gccaagattg tcaaggccat ttgcaagaca
     3601 aataaggtca ccaacatgga caagatacct gctgaaactt ggaacaactt accacccagt
     3661 tttttgctag gaatcaagtg taagagaaga ccaagcaaac gaccaatgct atagacaatc
     3721 ccaaatacaa gcaagtagcc gatgcctgca taaaagacct tatccagctg aaagagttga
     3781 tccgatggga aaaagaaagt cccctgacct tcctgcgaat ttgcataagg gagcaataaa
     3841 tggaattgct ctccaagccc cttataaaac tggccagcca taaaagccga tgccatggct
     3901 gaaatcaggt aataaacctg taagagcagg cctctccgat agccgatata aaatccccaa
     3961 accaagacca atagaagaag gaatgaaatc ataaggaatc ctcaatcttg ctctgttctt
     4021 gcttacaagt cacaagcttg tgacggagtt cttctagctc ttgctcctta tcgtcaaatt
     4081 caatctcacg gctgagctga gttgataaac agttgactgc caacaaaaga gcgattgttt
     4141 catcatctgc gctaggcatt tgttctttaa ttgcttggta tttttctgtc gcaaccttag
     4201 cgatttcctc cataaaaagg ttatcatgtt cgcttgtcaa ggttaacgat tttttcccga
     4261 atgtaaattt gaatcgattt agatttgcca taaaattcac ctcacgatat tataccaaaa
     4321 ttcgctaatt ttgtcagttt ttacaaattt tactgctttt gtggtacaat agaaactatg
     4381 gcaagtatca cactcacacc aagcgaaaag gatattcagg cttttcttga acactatcaa
     4441 accagtctgg ctcctagcaa gaatccctat atccgctact ttttgaaact acctcaagca
     4501 acggtttcta tctatacttc tggaaaaatc ttgcttcagg gtgaaggggc tgaaaaatac
     4561 gccagtttct ttggctatca agctgtagag caaaccagcg gacaaaatct tcctttaatt
     4621 gggacagatg aggtgggaaa tggttcctac tttggtgggc ttgcagttgt ggctgccttt
     4681 gtcacacctg accagcacga ctttttacga aaactcggtg tgggggattc taagactctg
     4741 accgaccaaa agatccgtca gattgctcct attctcaagg aaaaaatcca gcaccaggca
     4801 ctccttctct cacccagcaa gtacaacgag gtcatcggag accgctacaa tgctgtttcg
     4861 gttaaggttg ccctccataa tcaggctatc tatctcctcc ttcaaaaagg tgttcagcct
     4921 gagaaaattg tgattgatgc ctttaccagt gctaaaaatt atgacaagta cttggcacaa
     4981 gaggccaatc gtttcagcaa tcctatcagc ttagaagaaa aggctgaggg caaatacttg
     5041 gctgtcgcag tttcttctgt cattacgcgt gatctctttc tggaaaatct tgaaaatttg
     5101 ggacgagaac tgggttatca gcttccaagt ggagctggaa cggcttctga caaggtggct
     5161 agccagattt tgcaagccta tggtatgcag ggactcagct tctgcgccaa attgcacttt
     5221 aaaaacactg aaaaagcgaa aaaacgctta gaaaggtaag ttatgaattc atttaaaaat
     5281 ttcttaaaag agtggggatt gttcctccta attctgtcat tactagcttt aagtcgtatc
     5341 tttttttgga gcaatgttcg cgtagaagga cattccatgg atccgaccct agcggatggc
     5401 gaaattctct tcgttgtaaa acaccttcct attgaccgtt ttgatatcgt ggtggcccat
     5461 gaggaagatg ggaataagga catcgtcaag cgcgtgattg gaatgcctgg cgacaccatt
     5521 cgttacgaaa atgataaact ctacatcaat gacaaagaaa cggacgagcc ttatctagca
     5581 gactatatca aacgcttcaa ggatgacaaa ctccaaagca cttactcagg caagggcttt
     5641 gaaggaaata aaggaacttt ctttagaagt atcgctcaaa aagctcaagc cttcacagtt
     5701 gatgtcaact acaacaccaa ctttagcttt actgttccag aaggagaata ccttctcctc
     5761 ggagatgacc gcttggtttc gagcgacagc cgccacgtag gtaccttcaa agcaaaagat
     5821 atcacagggg aagctaaatt ccgcttatgg ccaatcaccc gtatcggaac attttaagaa
     5881 acctaagagg ccgagaatca ccaatctcag cctcttcttc tatcgtgaga aaatgattgg
     5941 taactatcta aacttaccag aacagaaaca cctcaactct cacctattca tgcaaaggaa
     6001 ttttatggaa gtttattttt caggaaccat tgaacggatt atttttgaaa atcccagcaa
     6061 tttttatcgc atcctcctcc tagaaatcga cgatacggac gcagaggatt ttgatgactt
     6121 tgaaatcatt gtcacaggaa ccatggctga tgtaattgag ggcgaagact atactttttg
     6181 ggggcaaatt gtccagcact ccaagtatgg agaacaactg caaatcagtc gttatgatcg
     6241 cgcaaaacca actagtaagg gcttggtcaa gtacttttca agtagccatt tcaaggggat
     6301 tggtctcaag acagctcaga aaatcgtgga tacctatggc gaaaatacca ttgacgaaat
     6361 tttgcaacac ccagaaaagt tagaaggcat cgcaggactc tctgccaaaa atcgcgaggc
     6421 tttcgtctcc actctccgtc tcaactacgg aacggagatg gttttggcca aactagccaa
     6481 ctacggcatt cccaacaaac tagcctttca gattcaagac ttttacaagg aagaaaccct
     6541 tgatgtggtt gaaaattatc cctaccagct ggttgaggat atcaagggtt tgggctttac
     6601 cattgctgac caactagctg aggaactagg catcgaaagt caggctcctg aacgcttccg
     6661 cgccggtcta gttcacagtc tttttcaggc ctgtatggaa acaggggaca cctatgttga
     6721 agcacgggat ttgctggaac aaacccttac tctccttgag tcttcccgtc ccgtggaact
     6781 agaccccagc caagttgccc aagagctctc ctacctgatc gaagaagaca aggttcagca
     6841 gattgatacc aagatctttg acaacagcct ctttttcgct gaggaaggca tccgcagtca
     6901 cttggttcgt atccttgaaa aaggaaaaca gaagagtcat gatttaaaaa ccattcaaaa
     6961 acatatcact actatcgagc aagagcttgg aattgagtat gataacattc aaaaacaggc
     7021 tatctgtgat gctatccaga ataaggtctt tatcctgaca ggtggacctg gtactggtaa
     7081 gacaactgtt atcaatggga tcatcgctgt ttacgccctg ttagagggac ttgacttcag
     7141 gaagaaaagc aacctgccca ttcttcttgc tgctccaact ggtcgagctg ctcgtcgcat
     7201 gaatgaattg acaggtttgc ctagcgcgac catacaccgc cacttgggaa tgacaggtga
     7261 cgatgatacc agtcatctgg aagattacct agatgctgac tttatcatcg tggatgaatt
     7321 ctctatggtg gatacttggc tggccaatca actcttctcc aatatctctt ctaacagtaa
     7381 gatcctcatc gtaggtgaca gtgatcagct accttctgtc agtcctggac aggttctagc
     7441 ggatctgctt catattcctt tgattcctca gactcgcttg gaaaaaattt atcgacaaag
     7501 cgaagaatca accatcgtca ccctagctag tcagattcga cagggcatct tgccagctga
     7561 tttcacccaa aaaaaagctg accgttccta ctttgaaatt gctagtggcc atattcctgc
     7621 caccattgaa aaaatcttag gtgccgccct cagaaatggt attcctgccc gtgatatcca
     7681 agttctggct cctatgtacc gagggacggc agggattgat gctatcaatc agctcatgca
     7741 agacctgctc aatcccccac aaaaagatca actcagtttt gaagctcctc agtgccacta
     7801 tcgtaaggga gacaaggtca ttcatttggt taacgatgct gaaatcaatg tctttaatgg
     7861 ggatttagga gctatcacag acctgattcc tggtaaatac accgaatcga aacaagacga
     7921 gattgtcatt gattttgatg gtaatgaagt ctcttacccc cgtaacgaat ggtacaagat
     7981 tcgcttggcc tatgccatga gtattcataa gtctcaggga agtgagttcc cagttgtcat
     8041 cctaccgatt actagtgcta gccgacgtat gctggagcga aatctcatct acacagccat
     8101 tacacgtgcc aaaagcaaac tcatcttact aggcgaatta caagccttcg actatgctac
     8161 ccaacacatc ggaactgccc gaaaaaccta tctgattgaa cgcttcagtg atctactgga
     8221 gaatgttgaa gaaaagcaac aagctgtctc tgaaacagtc acatcaagtg cctctgaaca
     8281 atcctacatc ctaaccgaag aaaactggga cagcattcca gccatgattg ggattacaga
     8341 cacagacctc aaagagattt ttggaaaata gtacatcaga aaacccacca attcaggtgg
     8401 gttttctgtt tattaagatt attttactgt tgctgtgctt gtgatcaatt caacagcttt
     8461 tttgatagtg atatcatgtt tcaacatgtc agctgaaagc aagttttgaa cttgtgcaac
     8521 ttccatgttg tagtctgctg ccaattgctc aacttctttt tggatttctt cttctgaagc
     8581 atcaaatcct tcagctttgg caactgcttc gataacaagg ttagtcttag tacgtgactc
     8641 agcttctgct tggtattggt tgtgaaggtc ttcttgagta gttccagtga tttggaagta
     8701 catgtcaggg ttgatccctt gacgttgcaa gttcccaagg aattcattta ctgaacggtg
     8761 aacttcttca tggatcattt cttctggaag ttctacgatt tcagcgtttt ctacagctgt
     8821 atcaattgct gcaccttcaa ctgcatcttt gtaagcttct tctttagcag cagccaattc
     8881 tttgcggtat ttttctttca agtcagcaag tgtttcaact tcttcatcaa tgtcttttgc
     8941 aagttcatcg tcaagagctg gaacttcttt agcttttact tcgtggatag ttgtcacgaa
     9001 tttagcttct ttacctgcaa ggtcttctgc ttggtagtct tctgggaatg ttacgataac
     9061 atcaacggtt tcgccagctg agtgacctac caattggtct tcgaaaccag ggatgaattg
     9121 acctgaacca agtccaagtg agaagttttc accttttcca ccgtcaaatt caacaccgtc
     9181 gatagaacca acgaagtcga tcacaacagt gtcgccgttt tcagcagcag cttccttgat
     9241 aaccaattca gccagattgt tgcgttcgcg ttcgatacgc tcttcaacat cagcgtcagt
     9301 tacttctttt tctacatcaa ctgatacttc aaggtttttg tagtcaccca attttacttc
     9361 aggttttgta acgacttcag cagcgataac ccagtcttga cctttttcca ttgaagttac
     9421 gtcaattttt ggttgggcaa ccacttcaag accagcttct tttacagctg cttcataagc
     9481 gtttggcaaa agagcgttca taacgtcttg gtaaagtgat tcttcaccaa attttttatc
     9541 gaagatagga cgtggaaggt gacctttacg gaaacctgga acattaagag atttcttcac
     9601 tgacttgaag acacggtcca attctggttt gatttggtct tgagagatag tgaaagtcaa
     9661 gacaccacgg tttgtttctt tgttttcaaa tgatacagac attctgtcat ttctccttaa
     9721 aattttttaa atacagtcta ttataacatt
//
LOCUS       DAVKLW010000072         9740 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKLW010000072 DAVKLW010000000
VERSION     DAVKLW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.072"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(386..2887)
                     /locus_tag="VW324_001825"
     CDS             complement(386..2887)
                     /locus_tag="VW324_001825"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9727396.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(3342..4430)
                     /locus_tag="VW324_001826"
     CDS             complement(3342..4430)
                     /locus_tag="VW324_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9727397.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(4448..5116)
                     /locus_tag="VW324_001827"
     CDS             complement(4448..5116)
                     /locus_tag="VW324_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9727398.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(5135..7573)
                     /locus_tag="VW324_001828"
     CDS             complement(5135..7573)
                     /locus_tag="VW324_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9727399.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(7724..9022)
                     /locus_tag="VW324_001829"
     CDS             complement(7724..9022)
                     /locus_tag="VW324_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9727400.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGTIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            complement(9039..9347)
                     /locus_tag="VW324_001830"
     CDS             complement(9039..9347)
                     /locus_tag="VW324_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9727401.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(9393..9713)
                     /locus_tag="VW324_001831"
     CDS             complement(9393..9713)
                     /locus_tag="VW324_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9727402.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         3006 a         2065 c         1599 g         3070 t
ORIGIN      
        1 tttcatttgg agatattcca aaaatattgc ttgatttatc atttgaaaaa ctaaagaaag
       61 taataaaaac tgcaaagaga gtgtctaaag tgagtgtcaa atttaaactt tccttgtccc
      121 aaagacaatt tttaaaataa tatgcaccca ctattgacat tactaatact ataactgaca
      181 ctacaataat agaaatctta aataataaaa tttcacactt cctgctatcg ttcactaatc
      241 tattcattct gacctattct aaaagtaact ctaaagttca tactctagag ttacttcttt
      301 ttatttcaca acaatgttaa ctaatttagt tggcttcaat agtccagtgg actattgaag
      361 gtggcagata gagctaataa actcgttatt taacgacgat attaaccaat ttatttggta
      421 cactaatcac tttcacgatt tccttaccgt caatttctgc tttgactttt tcatccgcta
      481 gagcaatttc ttgcaattct tcacgtgata ggtctttagc gaccatgagt ttggcacgga
      541 ctttaccttt gatttggaca acgatttcga tttcgtcttc aaccagttta ctttcgtccc
      601 atgttggcca agtcacgtaa gagattgact cacctgttgc agcaacagtt tgccagagtt
      661 cttctgccaa gtgaggtgca aatggggcaa tcaattggat aaatcctttg gcatagtcaa
      721 catagagctt gtcttccttg ttagccgcat tgacaaagac cataagttgg gcaatggctg
      781 tgttgaattt gagggaatca atttgctctg tgacagcttt aacagtttcg ttgtaaacct
      841 tgtcaagagc accattgttt tccgcaagga tttctttact tgtaatcaaa cggtaaactc
      901 ggtcaaggaa cttacggctt ccttccaaac cttcttctga ccaagcaatc gaagcatcga
      961 gtggtcccat aaacatttca taaacacgaa gggtatcggc accgtattgt tccaccacat
     1021 cgtctgggtt aacaacgttc ttgagcgatt tagacatctt ggctggcgct tgctccaact
     1081 cttcccctgt ttctacatga aagaaggaac catcacgttt ttcaaccttg tcggttgcca
     1141 caagagcacc acggtggtca cggtagcttg ttcccaaaat catcccttga ttaaagagtt
     1201 tttggaatgg ttccttagtc ggaacaacac cgaggtcata gaggaatttg tgccagaaac
     1261 gagcgtaaag caagtgaagt acagcatgtt ccgcaccacc cacgtagata tctactggca
     1321 accattgttt gaggaggtcc tcatcagcca atttctcagt attgtgtgga tcaatatagc
     1381 ggaggtagta ccagcttgaa ccagcccatt gtggcatagt gttggtttca cgacgacctt
     1441 tgacaccatc ttcacgagtc acttcaagcc aatctgtcaa gttagctagt ggactttcac
     1501 cagtacctga aggacggata tccttggtta caggcaagac aagcggcaat tcagtttcag
     1561 gaacagctgt tgaagttcca tcttcccaat gaatgattgg aattggctca ccccagtaac
     1621 gttgacggct aaagagccag tcgcggagac ggtaggtaac cttctcctga ccacagcctt
     1681 tttcttccaa ccaagccaca atcttggcaa tagcgtcttc tttgttgagt tcatctagga
     1741 agtctgaatt gacatgcagg ccatcctctg tgtaggcagc ttctttgaca tctccacctt
     1801 caagtacttc gacgattgga aggtcaaatt gtttggcaaa ttcccagtca cgttggtcgt
     1861 gggcaggcac agccataacc gcacctgttc cataactagc aaggacatag tctgcaatcc
     1921 agattggcat ttccttacca ttgacagggt tgatggcata ggcacctgtc caaacaccag
     1981 ttttttcctt agcaaggtct gtacgagcca agtcagactt aaggctggct tggtgtttat
     2041 agtctgctac agcttctgct tgctctgaac ttgtgatagc gtctactaat tcatgttcag
     2101 gagccaagac agtgaaagtc gcaccgaaaa gtgtgtccgg acgagtagta aagactgtaa
     2161 attccttgtc tgttcctttt actttgaaag ttacattggc accagttgat ttaccaatcc
     2221 agttgcgttg catatccttg atagactctg accaatctag ttcatctaag tcattgagca
     2281 agcgctctgc gtaagccgtg attttgagca tccattggcg cattggtttg cggacaactg
     2341 gatagcctcc acgctcagaa gttccgtcag gaagcacttc ttcattggca atggcagttc
     2401 ccaattcctc aacccagttt actggcactt cagcttcata ggccaagcct ttttcgtaaa
     2461 gcttggtgaa aatccattga gtccacttgt agtagtttgg atctgttgta ttgacttcac
     2521 gatcccagtc ataagaaaat ccaagcgcat tgatttgacg tttgaagttg gcaatgtttt
     2581 ccgctgtaaa ttctgctggg tcattaccag tatccatagc gtattgctct gcaggcaaac
     2641 caaaagcatc ccaacccatt gggtgaagga cattgtagcc ttgcgcacgt ttgtaacgac
     2701 tgaggatatc ggttgcagta taaccttctg ggtgtcctac gtgcagacca gctccagacg
     2761 gataagggaa catatcaagc gcataaaact taggttttga tgcatctgtt cctgtcttaa
     2821 atgtatgatg ttctgcccag tagccctgcc acttaggctc aatttcttta tgattgtaaa
     2881 aactcatggt ctctctccaa ttttgtgatg ttactattat accatttttg agggaatttg
     2941 aaatgtgttt aaaggaaaaa ctgtatactg tatctcatta attttgatga ctatgaattg
     3001 ctctatctcc cactttttat cccattgtta tgcagtaaat caaggagtaa gtcaagaagc
     3061 ttatctcgta tagcacaaaa tacttatttt agaccgaaga taaaactgga atgctaatta
     3121 atgtactcac aataaatagc cgactaccta tgtcgctttt tgatttatga agaacgtata
     3181 gtagactgaa tctgaaatag tacgaaacaa ttgctaaaac atttatagaa attaatttta
     3241 ctttcccaat cgatttgttc tcatcctatt tcaatcttct atagaattca atacagggat
     3301 caataaaaga ctaccatttg gaaaacagta gtccttattg tttattttga atttacatag
     3361 gcatctacag cgataatagc ttgagcaaca tctgaaggcg aaatcttaaa cggcatctga
     3421 tgaattgtct caccctccat agttgcttgt ttaccaactt ttattaaatc atcatatcca
     3481 acttgatcca aatgcatttc ttttagagtt gttggcatac caattttttt gtaaaactca
     3541 atatacttat caagttcttc tttaggtcta ttttccaata atagttgtac taaagttcca
     3601 taagctactt tttcaccatg tgttaaatga tgaatgtcac ctgtcaatgc agtaaaacca
     3661 ttatgaattg catgcgccgc agctaatcct ccactttcaa aacctagacc actcaataaa
     3721 gtattagctt caacaatatt ttctaatgct ggtgtcacca ctttagcttc acaagctgcc
     3781 atagcctgta aaccatctgc aaacagcgtt tcttcacatt tcttcgcaat tgcaactcca
     3841 gccaatgttt gctgttgtcc caacatagtt tttccatttg cctgcataac cgcacgcgcc
     3901 tcaacccaag ttgctaaacc atctgcaata ccagacgcta ataaacgctt aggggcttgt
     3961 gaaataactt ttgtatcaac caaaactaaa tctggatttt tagaataaaa tagataatga
     4021 tcaaatgcac cttcatctgt ataaataaca gataaagcag atacaggtgc gtcggtcgat
     4081 gcaattgttg gagcaataat aacaggcttt tcaatcaaat ctgcaatagc ttttgcgcta
     4141 tcaatcgtct ttcccccacc aagaccgata atactatcac aattttcttt ctcagccaag
     4201 gcaacaactc gattgatttc attgtcagaa gcttcaccat taaatagcgc cagaacaata
     4261 tggaaaccat acctatgtag gtaatcttca aatcgttttc caacaatatc ataaaccaac
     4321 tgatcgcata atagaatagg gcaatttccc aaatccaaaa ttgatttggc attttcaaac
     4381 aaggcatttt ccccctgaat atatctagaa ggactagcaa aaattctcat atgtatttcc
     4441 tctctatcta aatggaacga ctattttgaa taacaaacca atcgtcagaa aaatcatcaa
     4501 ccgccttttg gatagatggc atggcgaaag ctgattcaaa aacatccgct cctgctgtaa
     4561 cagcatgcgc acctgcagct aaagcattat ttacttgtgc tacattttta aaggatgcag
     4621 ctaaaatctt actaggagag ttctgtctat caatagcaag agctaattga cgaatgacag
     4681 aatttgaatc aatgttcaga ttttccattc tattataata tggagctagg taatccgctc
     4741 ctgcttcgat agctaataat ccctgaataa ctgtataaat agctgttgca gtgatatggt
     4801 agccctcttt ttttagcgcc tttattgcac gtaatccagc tggagtaaca ggtactttga
     4861 taaatatatc atctcctgct tgtcttcgaa ttttatgagc atcctttaag atgccttcaa
     4921 aatcttgaga aatcacctga acatgaatag agggtgtaga gccaatcaat tctcttacat
     4981 ctttgattcg ttcaaaaaaa ttaatagaac cctctctttt tgcaatagtg ggatttgaag
     5041 ttaccccagc tagcggcaaa atttcagacc actttttaat ctcatctaaa tttaatgtgt
     5101 caagcataaa ttccataaaa agaacctctt tattttacaa agtatgctca gtacgtccta
     5161 taatgtcatc ttgggttgcc ttagaaagaa cattgaagaa tgcagagtat cctgcaacac
     5221 gaacaattaa gtctctgtgt ttttcaggat gtttctgagc gtcaatcagc gtctctctgg
     5281 aaacaacatt gtattgaata tggtacccat gtaaacgatt aaagaatgtt cgtaacaaag
     5341 caattagttt taatttatct tcttctttgg ctaacgtttg aggatttact ttctgattta
     5401 agagaacccc acctacgatt tcatctgttg gtaattttga aacagatttt aaaacagatg
     5461 tagggccgtg ttgatccata ttatgtgatg gtgaacaacc ctctgctaac ggtgtacccg
     5521 cgttgcgtcc atctggagtt gctaatgttc cacgtccctg ccctacgttg gctgagatag
     5581 aagatgttcc tgaataacga attcctccaa ttggacctct tccataacgt gtattaggat
     5641 atttagcaat ttcatcaaca taaatgtcat aagcagcagt aaccaatttg tcagcataat
     5701 catcatcatt accatactta ggtgcatcat gaatcaacat ttcttgaatg accttacctt
     5761 cttctccggc ataatctgtt tccagtgcat gccaaagctg acttgggctt atacgttctt
     5821 cctcaaacac caatttttta attgcagcta atgaatccga caaatttgca attccaactt
     5881 gcaatcctga tatataatca tatactgctc caccttcttt aaggtgtttt ccacgaccaa
     5941 tacaatcatc agtcaatgct gaacatagaa tatcaggaac ttctcgttcc aatgataaat
     6001 caatagaatt ttcaacaata acactcattc gtgtcaaata tcttagtgtt ttatcccaag
     6061 cattttctaa ttcagaaaag ttcttcatat ccttaaaatg accaaagctt ggtgcaaacc
     6121 gtttacccga agccggatca attccatcat tcatcgtgat aagtagaacc ttagggaagt
     6181 tcatataact catacctgtg caacgatagc cccatttccc tggaactgcc gtttcaacac
     6241 atccaatggc actgtaatca taagcatcat cttccaatac tccttttgca ataaaagaag
     6301 gaataataat ctcatcatta ttaaatgcag gcataccaaa accaagtttc atcacttcaa
     6361 tacactcatt catgaaacga gcatctaaac ctgcatggta acgtacagtt agattaggtt
     6421 gcggtagatg ggtttgtgca actgatttta ataccaaata agataatggg ttaacagcat
     6481 ccttcttatc tcgagtctgt ccaccaattg taacattttg atataaagga cttcctgctg
     6541 aagaaaatgt atgtgattga ctgcgaacct tattaattgt aattgtctta atccaaagat
     6601 ttgtcagacg ttcaacaatg ctatcttctg tttctttacc actttctaaa tcagccttca
     6661 tatatggata catatattga tcaaaacggc catatgaaag agagtggcca ttagattcaa
     6721 tttgtaaaat acattgaata aaccaaactg attgaatagc ttctgcaaaa gtagttgccg
     6781 gttcatatgg gactctagag caaatatctg caatctcaag taattctttc ttacgtttag
     6841 gatttgcatt ttcggctaaa cttttagcaa gagcaacaaa gcgctttgca tatactttaa
     6901 tagcatcgat tacgataaat atagagtcgt aaaaatgata tttatcaata cttgctggat
     6961 ctgttaaatc tagagctgct tttgctttac gagcccgctc ttcaaaacct cttaagccaa
     7021 attgcaaaag tttctgatag ttaactgcta ggtgagcatc tccagaattc atcttacctt
     7081 ccataccgaa gaatcctgtt tccatataaa cagacacttc ttcaggtaat aaggcaccag
     7141 ctctagcacg taaattatta ttttcccaaa acggagcaat acttctaagt tgttcttttg
     7201 tttcttctgt aatatagaaa acatctccat cacgcttttc aaaaagatcc aactcattga
     7261 gaacaaattc tagcgtatat tccggaaaaa taggagcatc tttattggaa gaagcttgat
     7321 ttcccgcaat catagattct tcttcaatat agatagtcat attttccaaa atttctttca
     7381 gcatatatgc acgttttagg acattaggtt gttccttgta tcgttcatag gcgcgtgttg
     7441 ctagaactgc tctctcagca tcaatataag gttttttatt taaaacatct tctcgatatt
     7501 tgttcatcct ttcagtaagg ctgccaaaat attctgtctt gattgttgtt cttgctactt
     7561 ctgtatttac catcgtaacc acctttcttt cgaaaatatt tttatttctt tcgaaaatat
     7621 aataccgatt tttatagaaa ttttcaagct tttaactaga aaaattttaa ctctctaatt
     7681 tttagcacaa atgtgtgagt ttaacagtaa cacacaaata cctctaagat tgtttgattt
     7741 cattttggta agctaaacga tcctgtactt taaagaatgg aaaataaacc aatgtagaca
     7801 tcgctaatat caccagctga gtaataactc cttgccatcc acccaccaaa aatcctgata
     7861 aaatagctgg tgtactccaa ggcaatgtta cccctgagaa tggctgcatg aaacctgttg
     7921 caatagttcc atatactatc acagctgcaa gtacaggaac aagaatgaaa ggtacaaaca
     7981 taactggatt catgacaatc ggaaatccaa atacaactgg ctcatttacg ttaaatattg
     8041 ctggaaaagc tgcaactttt cctaaggctt ggtattgttt tgattttgct gcaaaaagca
     8101 tggcaactac aagaccaaac gtaatccctg aacctgatag aattaaaaat gaatctaaaa
     8161 attgttgagt aacaatatgt gcaccatttt ctaatgatag attagcagag gctaacatag
     8221 ctttattagc atcaagatta gataaaagca gagctgtcac tactccattt actaccgatt
     8281 gtccatgaac accaaaccac cacaaaaatg atataaagaa tgcaattcca attgcaccat
     8341 acaaagatcc agttaaacct tgcaatggaa cttgaatagc agaataaatc atttctatga
     8401 atgttccgcc attagtcaat gacttcgcta aaatatatac aatcatagaa agtaagaaaa
     8461 ttacaaatgc tggaatcatt gcttcaaact gtttggcaat agcttgtgga acttgttctg
     8521 gcatcttaat aacaattttt ctctttataa agaaggtata aatacttcct actaccaaac
     8581 ctataatgat agcaccgata attccttgac ctccaaacca aactttacta atagcatccc
     8641 caattgcctc accttgttta gggatataag atgatcttag caaaataaag aatgcagata
     8701 cagatagaac tccagctggt aaagcctcta ctccgctatt cttagcataa gaataggcaa
     8761 ttgaaaaaca agaaattaga cccataatag caaaagttcc tgaatatact tgcataaacg
     8821 gctctgtcca attagctcca aaaacactag caatgctctt atttaatcct tcgaacggca
     8881 attgtcccat aatcaagaat aaactaccaa ctactgtcaa tggcaaaatt gctaacatcc
     8941 catcttttag agctataatg ccacgcatat tcacaaactt catcatcggt gcaatgattt
     9001 tctgaacatc catctttgcc ataataaatc tccttttctt acccactaat caaagatagg
     9061 gccaaatcta atactttttt cccatctaac ataccatagt ccatcatcgg aataacagct
     9121 atcggaacat cacacttatc acaaatttct tttgatttat ctaatgtata agcaacttgt
     9181 ggacccaata gtgcaacatc tatatttggc gcataatccg ctaatttaga ctgagaaaac
     9241 gcctctattt ctgcctcaac tccactagct tgcgctgcaa ttttcatatt atttacaagc
     9301 ataccagtag aaaaacctgc tgcacaaaac aaaccaatct tcaccattat gttttcctcc
     9361 tctatgttaa taacaatgat aatactctag tattattttt tatgaagttc ttttctcaaa
     9421 ctaataattt ctttgattaa attaatctcc gtcatactag tcatgagatg atcttgtgaa
     9481 tgaacaaaga gagctgtaat ttctgtcttt gtaccagacg cttcctgtgc caagaatttt
     9541 gtttgtagat tatgagcttc taataaggct gcatcagcta actggatttc ttcttcacat
     9601 cgttcacatg taccgttctt tatgtaatct aatgctttat aaatatgttg tttcgcatcg
     9661 ccagcatata aaattaaacc catgataatt tgatctggaa caataatttc cataaattcc
     9721 tcctcatttc actatgtaaa
//
LOCUS       DAVKLW010000073         8980 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKLW010000073 DAVKLW010000000
VERSION     DAVKLW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.073"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(1..917)
                     /locus_tag="VW324_001832"
                     /pseudo
     CDS             complement(1..917)
                     /locus_tag="VW324_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1173..2018
                     /locus_tag="VW324_001833"
     CDS             1173..2018
                     /locus_tag="VW324_001833"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9727403.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(2165..3226)
                     /gene="dinB"
                     /locus_tag="VW324_001834"
     CDS             complement(2165..3226)
                     /gene="dinB"
                     /locus_tag="VW324_001834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9727404.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            3619..5943
                     /gene="pflB"
                     /locus_tag="VW324_001835"
     CDS             3619..5943
                     /gene="pflB"
                     /locus_tag="VW324_001835"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9727405.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(6001..7263)
                     /locus_tag="VW324_001836"
     CDS             complement(6001..7263)
                     /locus_tag="VW324_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9727406.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(7528..>8980)
                     /gene="rlrA"
                     /locus_tag="VW324_001837"
     CDS             complement(7528..>8980)
                     /gene="rlrA"
                     /locus_tag="VW324_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU9727407.1"
                     /translation="ELDELSTLTSLQSKSLLSILQELQETFEEELTFNLDTQQVQLIE
                     HHSHQTNYYFHQLYNQSTILKILRFFLLQGNQSFNEFTQKEYISIATGYRVRQKCGLL
                     LRSVGLDLVKNQVVGPEYRIRFLIALLQFHFGIEIYDLNDGSMDWVTHMIVQSNSQLS
                     HELLEITPDEYVHFSILVALTWKRREFPLEFPESKEFEKLKNLFMYPILMEHCQTYLE
                     PHANMTFTQEELDYIFLVYCSANSSFSKDKWNQEKKTHTIQLILQHTRGKHLLSKFKN
                     ILGNDISNSLSFLTALTFLTRTFLFGLQNLVPYYNYYEHYGIESDKPLYHISKAIVQE
                     WMTEQKIEGVIDQHRLYLFSLYLTETIFSSLPAIPIFIILNNQADVNLIKSIILRNFT
                     DKVASVTGYNILISPPPSEEHLTEPLIIITTKEYLPYVKKQYPKGKHHFLTIALDLHV
                     SQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYLL"
BASE COUNT         2652 a         1676 c         1830 g         2822 t
ORIGIN      
        1 tcttcctgct aaatcaatct gacctgactg ataaatagaa gaatccagtc cagcgaaagc
       61 ttgtaattga gcaggattat caaaggcatg aatatttcga atctcggcta aaatgaccgc
      121 ccctaaacga ttctcaatcc cagtaaccgt cgtgatgacc gagttgaact cagccatcaa
      181 gtcattgaca cattttttcg ccttgtcaat gagcctcttg taatgtttga tgttttcgat
      241 gatttcgaat tcacgagcag gagatgttgt tccgatagaa cgaggtgcga ctgagaggat
      301 atcctgaatt ttagaagcgg tcaatcgctt aatttctatc agcttatcaa atcctgcctc
      361 aagccttttc tgaggattag ggtagcatgt caagagttgg taggtatatt ctgaatgctt
      421 tccaacgatt ttatccaact caggaaagat gatatcaaga caacgagtgt attgcacttt
      481 ccaatcagac tgttttttct tgagacgatg aatatgtcta gccagtattt ttagttctac
      541 ttgccgatta tcgtgttgaa attgttcacg attggggtca gaaagaagtt taagagcgat
      601 gccatgagcg tctttcttat ccgttttagt cttgcgaagt aataatgatt tggcaaattt
      661 cttgatgagc aaaggattgt aggtgtaaac tttatatcct tgttcatgca ggaagttcag
      721 tagattaaag gcataatgtc cggtatcttc aagagcgatg agatagtctt ggtcgagctg
      781 tcgaagagac agatctaaaa gttcaaaacc agctttatta tttgaaaaag tgagtggttt
      841 aagaacagtt ttcctcgaac attcaaggct gtaacatcgt gtttattttt agcgacatca
      901 atgcccacat aaagcatggg agtacctcca gatatagtat ttcaagtcta cttggttatc
      961 cacgaacttt ttgccttgtt accttagacg agataaaacg tctatgcgtt atcaaactca
     1021 ttaccaattg aaacaaaaag ctgtggttag accctttcgg aaatcgtcaa gcgattggag
     1081 gaaatgaact aatccacagt ggcttattcc aagtatacca cttgggcttt ggcagtagct
     1141 aactgcgtta aatataatat aaggagaaat atatgtatct tattgaaatt ttaaaatcta
     1201 tcttcttcgg gattgttgaa ggaattacgg aatggttgcc gatttccagt acaggtcact
     1261 tgattttagc agaggagttt atccaatacc aaaatcaaaa tgaagccttt atgtccatgt
     1321 ttaatgtcgt gattcagctt ggtgctattt tagcagttat ggtgatttat tttaacaagc
     1381 tcaatccttt taaaccgact aaggacaaac aggaagttcg taagacttgg agactatggt
     1441 tgaaggtctt gattgccact ttgcctttac ttggtgtctt taaatttgat gattggtttg
     1501 atacccactt ccataacatg gtttcagttg ctctcatgtt gattatctac ggggttgcct
     1561 tcatctattt ggaaaagcgc aataaagcgc gtgctatcga gccaagtgta acagagttgg
     1621 acaagcttcc ttatacgacc gctttctata tcggactctt ccaagttctt gctcttttac
     1681 cagggactag ccgttcaggt gcaacgattg tcggtggttt gttaaatgga accagtcgtt
     1741 cagttgtgac agaatttacc ttctatcttg ggattcccgt tatgtttgga gctagtgcct
     1801 taaagatttt caaatttgtg aaagccggag aactcttgag ctttgggcaa ttgtttttgc
     1861 tcttggtcgc gatgggagta gcttttgcgg tcagcatggt ggctattcgc ttcttgacca
     1921 gctatgtgaa aaaacacgac ttcacccttt ttggtaaata ccgtatcgtg cttggtagtg
     1981 ttttgctact ttacagtttt gtccgtttat ttgtataaga aaaaccttga aggggcaact
     2041 cttcaaggtt ttatactctt agaaaatctc ttcaaaccgc gtcagcttta tctgcaacct
     2101 caaaacagtg ttttgagcag cctacggcta gcctcctagt ttgctctttg attttcattg
     2161 agctttaaaa tccagtcatg gtaatcccca ataggcggac acctctttct ttctcgctta
     2221 attcttcata gagttgcagg gctatttggc ttatctgact agcatcttgt gttttttgag
     2281 caagactttt tcgtttggta agagttgaaa agtcctcgta gcggattttc aaaatgacaa
     2341 tttttccagc tttttcttgt tgatgtagat tgagagcgac tttttctgat agaagagtca
     2401 gctctttttt gatatcttcc tcggcacgga gaatcttccc gtaggttttc tccttgccga
     2461 ttgatttacg gatgcgattg gatttgactg gagagttgtg aatgccacga gcctttcgat
     2521 atagatcata gcctagtcta ccaaaacggt ctattagggt cacctcagga acttcaagta
     2581 aatcagcacc agtaaaaacg cccatttgat gaagacgttc tactgtcttt tttcctactc
     2641 catgaaattt ggaaatatcc atttgtttga gaaaatcctc agcctgttca ggtagaatca
     2701 ctgtcaaacc atgaggtttt tgataatcac tcgccatttt agctaagaac ttgttgtagg
     2761 aaataccggc agaagcagtt agatggagtt cttgccagat atctttttga atgaggcgag
     2821 caattttgac cgctgacttg ataccaagtt tattttctgt cacatccaaa taggcttcgt
     2881 caatgctcat gggttcaatc aaatctgtat agcgcttaaa aatagctcga atctggagtc
     2941 ccacagcttt gtatttctca taatttcctg agataaagac ggcttgggga caacgttcat
     3001 aagcttcctt ggaactcatg gcagaatgga caccaaaagc tcttgcctca taactacagg
     3061 tagaaacgac tccccgtcca cctgtttgcc gagggtcgct tccaataatg acaggttttc
     3121 ctctgagttt aggattatcc ctgatttcca ctgcagcaaa aaaggcatcc atgtcaatat
     3181 ggatgatttt tcttgacaaa tcatttaaca aaggaaaaat caacatgcct agcacctttt
     3241 tatactcttc gaaaatctct tcaaaccccg tcagctctat ctgcaacctc aaaacagtgt
     3301 tttgagctga cttcgtcagt tctatttaca acctcaaagc agtgctttga gcagcctgcg
     3361 gctagcttcc tagtttgctt ttcgatttcc attgagtgtt actgcttatt ttcttttatt
     3421 ataccctttt ttctgaaaaa aagaaaaaag gactttattt tttcaaaaat ataatacagt
     3481 ttgaaataaa atatagactg ttttagaaaa gaaagtttaa aaatagggat ttttcacttg
     3541 ttgaaatcgg ttactatatg gtatacttgt ctcatgaatg taacagatga ctgttactag
     3601 aaaaaagagg acattaatat ggttgttaag acagttgttg aagcacaaga tatttttgac
     3661 aaagcttggg aaggcttcaa aggcgtagat tggaaagaaa aagcaagtgt atcacgcttt
     3721 gtacaagcta actacacacc ttatgatgga gacgaaagct tccttgcagg accaacagag
     3781 cgttcacttc acatcaagaa aattgtagaa gaaactaaag cacactacga agaaactcgt
     3841 ttcccaatgg acactcgtcc aacatctatc gctgatatcc ctgctggatt tatcgacaaa
     3901 gaaaatgaag ttatctttgg tatccaaaat gatgaactct tcaaattgaa cttcatgcca
     3961 aaaggtggta tccgtatggc tgaaactact ttaaaagaaa atggatacga accagaccca
     4021 gctgttcacg aaatcttcac taaatatgta acaacagtta acgacggtat tttccgtgcc
     4081 tacacttcaa atattcgtcg cgctcgtcac gcacacactg taactggtct tccagatgca
     4141 tactcacgcg gacgtatcat cggtgtttac gcacgtcttg ctctttacgg tgcagactac
     4201 ttgatgcaag aaaaagtaaa tgactggaat gcaatcaaag aaatcgatga agaaacaatc
     4261 cgtcttcgtg aagaagtaaa ccttcaatac caagcattgc aacaagttgt tcgcctgggt
     4321 gacctttacg gggttgatgt tcgcaaacca gcgatgaacg tgaaagaagc aatccaatgg
     4381 gttaacattg ctttcatggc tgtctgccgt gtgattaacg gtgctgctac atctctaggt
     4441 cgtgtaccaa tcgtattgga catctttgca gaacgtgacc ttgctcgtgg tacatttact
     4501 gaatcagaaa tccaagaatt cgttgatgat ttcgttatga aacttcgtac agttaaattt
     4561 gctcgtacaa aagcttatga ccaattgtac tcaggtgacc caacctttat cacaacttct
     4621 atggctggta tgggtaacga cggtcgtcac cgtgttacta agatggacta ccgtttcttg
     4681 aacactcttg acaacatcgg taactctcca gagccaaact tgacagttct ttggactgac
     4741 aaattgccat acaacttccg tcgctactgt atgcacatga gccacaaaca ctcttctatc
     4801 caatacgaag gtgtaacaac aatggctaaa gacggatatg gtgaaatgag ctgtatctca
     4861 tgctgtgtgt ctccacttga tccagaaaat gaagaacaac gccacaacat ccagtacttc
     4921 ggtgctcgtg taaacgttct taaagccctt cttactggtt tgaatggtgg ttacgacgat
     4981 gttcacaaag actacaaagt atttgacatc gatcctattc gtgacgaagt tcttgaattc
     5041 gaatcagtta aagcgaactt tgaaaaatct cttgactggt tgactgacac ttacgtagat
     5101 gccttgaaca tcatccacta catgactgat aagtacaact acgaagctgt tcaaatggcc
     5161 ttcttgccaa ctaaacaacg tgccaacatg ggattcggta tttgtggatt tgctaacact
     5221 gttgatacat tgtcagcgat taaatacgct acagttaaac caatccgtga tgaagatggc
     5281 tacatctacg attacgaaac aatcggtgac tacccacgct ggggtgaaga tgacccacgt
     5341 tcaaacgaat tggcagaatg gttgatcgaa gcttacacaa ctcgtctacg tagccacaaa
     5401 ctatacaaag acgcagaagc tacagtatca cttttgacaa tcacatctaa cgttgcttac
     5461 tctaaacaaa ctggtaactc accagttcac aaaggtgtat acctcaacga agatggttct
     5521 gtgaacttgt ctaaacttga attcttctca ccaggtgcta acccatctaa caaagctaaa
     5581 ggtggttggt tgcaaaactt gaactcactt tctagccttg actttagtta tgcagctgac
     5641 ggtatctcat tgactacaca agtatcacct cgcgctcttg gtaagactcg tgatgaacaa
     5701 gttgataact tggtaacaat ccttgatggt tacttcgaaa acggtggaca acacgttaac
     5761 ttgaacgtta tggacttgaa cgatgtttac gaaaaaatca tgtcaggcga agacgttatc
     5821 gtacgtatct ctggatactg tgtaaacact aaatacctca ctccagaaca aaaaactgaa
     5881 ttgacacaac gtgtcttcca cgaagttctt tcaatggatg acgccttgga tgcattgagc
     5941 taatcaagtt cttgaataat aaaaagggct ctttgtcaac tgtagtgggt tgaagaaaag
     6001 ctaagctcga gaaaggacaa attttgtcct ttcttttttg atgttcagag cgatgaaaat
     6061 ccgttttttg aagttttcaa agttccgaaa accaaaggca ttgcgcttga tgtctttgat
     6121 gagtttgtta gtggcctcaa gtttagcgtt agaataaggc aattcaatgg cgttagtgat
     6181 gtagttttta tagcaaataa atgtgctcaa agtggtttta aaggtgcggt tgagatgagg
     6241 taacgtgtct tgaattaagc cccaaaactg gtcagtattc ttctcttgta gatgaaatag
     6301 gagtagttga tacaggtcat agtaatcttt aagttcaggt actagagtaa agattttctt
     6361 cagacactcc ctaggagtta aggtctctct gaaagttcta gcatagaaag gcttaagaga
     6421 gagtttccga ctatctttta ggataaattt ccagtaatat ttaagagctc tgtattccag
     6481 agatttatca tcaaattgct tcatgatgtt gattctagtc tgattaagag ccctgctcat
     6541 gtgttggaca atgtggaaac gatcgagaac aattttagca ttgggaaata atttcttaat
     6601 gagagggata taacttccag acatatcaac agtgacgact ttaacttttt ttctagcttc
     6661 tttcgagtac ttgaagaaat gatttcggat ggttgtttga cgtctgttat caagaatggt
     6721 catgattttc ttagtgttga aatcctgagc aatgaaagcc aatttcccct tctggtagga
     6781 gaattcatcc caggagagga tttcaggcaa agtggtgtaa tcctcttgga aatgaaattg
     6841 cttgagctta cgatagacgg tagaggtaga ggtagagatg gctaatttag aagcgatatg
     6901 tgtaagagcc tctctgttga gtaggagttg ggcaattttc tgtctcacca tttccgagat
     6961 ttggcaattt ttctgaacga gagttgtttc agctacagtg actttccgac aggacttgca
     7021 ttgaaatcgt ctctttttca aatgaatgag gctagggaaa ccaccaatct cgataaaagg
     7081 gattttagaa ggcttttgga agtcgtattt gatttgtttt cctttacagt gtttacattt
     7141 aggtgggtga taatcaagtg tagcgaagac ttcgatatgg gtatcgtgct gaatggcttt
     7201 atttaaggtg atgtttttgt cttttattcc gatgagtaat gtggtatgat tgatgtgttc
     7261 cataagatac tttctaatga gttgtttagg cgcttttcat tataagtctt atgggacttt
     7321 tttgatactc aaaaagccct ataatctcca cagtgggatt tacccactac agaaattata
     7381 gagccagaaa aaacactttt gttcactagc agaaactaga gagcagaagt gtttttctgt
     7441 tcagatttac ccaaaactgg gaaatatggg gataagaata gagatggctt aggaagcccc
     7501 tttttgtgtg tagacagtac gatgaactta taacaaatag tgagcctttt tagcaatcat
     7561 tgcgacccgt ttgtcaaaag cctcttttcg gatatctaca attgtctgat agatgagacg
     7621 ctgttggcta acatgcaaat ctaaggcaat cgtcaaaaag tgatgtttcc ctttgggata
     7681 ctgcttttta acgtaaggca ggtattcttt cgttgtaata ataatcaatg gctctgtcaa
     7741 atgctcctct gaaggaggag gactaattag aatattgtat cctgtaacag aggcaacttt
     7801 gtcagtaaaa ttccgtaaaa taatggactt tattaagttt acatctgctt gattatttaa
     7861 aatgataaaa atcgggatag caggtagtga ggaaaagatg gtttctgtca agtagagtga
     7921 gaaaaggtac agccgatgct ggtcgataac tccttcaatc ttctgctcag tcatccactc
     7981 ttgaacaatt gctttcgaaa tatgatacag tggcttgtcg ctttcaatcc cataatgttc
     8041 gtaataatta taatagggaa ctagattttg taaaccaaac aaaaacgttc ttgttaagaa
     8101 agtcagtgct gttaaaaaag aaagagaatt cgaaatgtca tttcctaaga tattcttgaa
     8161 cttggatagt agatgctttc ctcttgtatg ctgaagaatc agttgaatag tatgagtctt
     8221 tttttcttga ttccatttgt ccttggaaaa cgaagaatta gcagaacaat aaaccaaaaa
     8281 gatataatcc agttcttcct gagtaaaagt catgttggca tgtggctcta agtaagtttg
     8341 gcaatgttcc atcaaaatcg gatacataaa gaggtttttt aatttttcaa actctttgga
     8401 ctcagggaac tcaagtggaa attcccgacg tttccaagtg agtgccacta gtatgctaaa
     8461 atgaacatac tcgtcaggtg tgatttctaa cagttcatga ctgagttgag aattagactg
     8521 cacaatcata tgtgtgaccc aatccatact tccatcattc aaatcataaa tctcaatacc
     8581 aaaatgaaac tggaggagtg caattaaaaa acgaatgcga tattcaggac caactacttg
     8641 atttttcaca aggtccaaac ctactgaacg tagtaacaag ccacactttt gtcgtacgcg
     8701 gtagcctgtt gcgatggaaa tatactcttt ttgtgtaaat tcgttaaagc tttgattacc
     8761 ttgtagtaga aagaagcgga gtatttttaa aatagttgat tggttataaa gctgatggaa
     8821 gtaataattc gtttgatgag aatggtgttc gattaattga acttgttgcg tatctaaatt
     8881 aaatgtcaac tcttcctcga atgtttcttg taattcctgc aaaatgctta ggagactttt
     8941 agattgtaat gaagttaaag tagacagttc atctagttca
//
LOCUS       DAVKLW010000074         8946 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKLW010000074 DAVKLW010000000
VERSION     DAVKLW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.074"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(310..939)
                     /locus_tag="VW324_001838"
     CDS             complement(310..939)
                     /locus_tag="VW324_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9727408.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVCYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGFPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGW"
     gene            complement(984..1223)
                     /locus_tag="VW324_001839"
     CDS             complement(984..1223)
                     /locus_tag="VW324_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727409.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1373..1639)
                     /locus_tag="VW324_001840"
                     /pseudo
     CDS             complement(1373..1639)
                     /locus_tag="VW324_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1664..1975)
                     /locus_tag="VW324_001841"
     CDS             complement(1664..1975)
                     /locus_tag="VW324_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002876249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9727410.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKTMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(1900..2142)
                     /locus_tag="VW324_001842"
                     /pseudo
     CDS             complement(1900..2142)
                     /locus_tag="VW324_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2143..2775)
                     /locus_tag="VW324_001843"
     CDS             complement(2143..2775)
                     /locus_tag="VW324_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9727411.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(2789..3784)
                     /locus_tag="VW324_001844"
     CDS             complement(2789..3784)
                     /locus_tag="VW324_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9727412.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(3781..4479)
                     /locus_tag="VW324_001845"
     CDS             complement(3781..4479)
                     /locus_tag="VW324_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9727413.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4556..5566)
                     /locus_tag="VW324_001846"
     CDS             complement(4556..5566)
                     /locus_tag="VW324_001846"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9727414.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(5550..6557)
                     /locus_tag="VW324_001847"
     CDS             complement(5550..6557)
                     /locus_tag="VW324_001847"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9727415.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRSFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6544..7497)
                     /gene="mvaD"
                     /locus_tag="VW324_001848"
     CDS             complement(6544..7497)
                     /gene="mvaD"
                     /locus_tag="VW324_001848"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9727416.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7479..8357)
                     /gene="mvk"
                     /locus_tag="VW324_001849"
     CDS             complement(7479..8357)
                     /gene="mvk"
                     /locus_tag="VW324_001849"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9727417.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8479..8808)
                     /locus_tag="VW324_001850"
     CDS             complement(8479..8808)
                     /locus_tag="VW324_001850"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9727418.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TSCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2624 a         2077 c         1598 g         2647 t
ORIGIN      
        1 ggactatcca aaaacacttc gttattggac gattacatta tagtaaaacg aaataagaac
       61 aggacaaatc gatcaggaca gtcaaatcaa tttctaacaa tgttttagaa gtagaggtgt
      121 actattatag tttcaatcta ctatacaaga cgaaattgaa gaaatctttc cattataaaa
      181 cgcatattat caaggttttt aaacacctaa taatatgcgt tttttcctat tttaaagact
      241 tttatccacc tattatacaa agttaagtat ccatcacagt ttacaattag ccttcattac
      301 ctaacccatt caccatcctt caagagagta acctttaaga accgaataaa taaatgacaa
      361 atttcgttca tttaatttaa ctgcactgtt aatttgatta gctccatctc ctaaagtatg
      421 cactcctact acacggtgac tagcatcata aactgtagat ccactagacc cctctaaagt
      481 atcaacttgg taatccaaga acatgccatc atcacttaaa acttgttttt tatctgtata
      541 catttgatga attttaaaat tatatgatgg atagcctgtg atagtcacag ttattcctgt
      601 caaatttttt tgactagtag gaaaacccaa agtccctaat tttgcaccaa tgggctcttc
      661 tagaattaat aaagccaagt catattccct tgcatcctta gaatttaaat ttctaaattc
      721 cttcaaataa caaacttcct ttactttgat ctttccaaat agttcttgac ttggactaac
      781 agccggaaga acataaatat catccgcttc tttcccatag tcatgtctgt agtagttgtg
      841 agccgctgta attaacacat tatctttaat aaaattcgca cttcccctcc cttctacatt
      901 cattccatcg gctactccat aatacttaga taaaaccata gctgaagtcg aatacggata
      961 ctgtaaagta ttatcaattt taatcaaatc atcattaccg ataatacttc tgattgcttt
     1021 tggtagtatg aaccatacgt tggtgaaatc tcagataatg aagaatcatt agaccctgga
     1081 cctttttcta gtgtctcact tacctcatat tcttcacctg tactagaaat aacactcaaa
     1141 gcagatactg tcgataactg gctagccaat aaagtactcg caataattga aatacccaat
     1201 tttttataaa cagttttctt cattattgta tcctcctaat gtaattatag cgtactattc
     1261 taaatttctt aatctactat agaatcaaga aatctaccac cttctttaaa taccctccat
     1321 tatcacataa acaggtaaac ttttcaatta atgactgcgc ttttcaatca cgctagaggt
     1381 acttacttgc ttctttgata ctaagttcag ccattctttc cttgtttttc tcaataaagc
     1441 atgttaccca agtgggattc gttttggagt agtctcgcag agtccagcca atggctttat
     1501 tgataaaaaa ttctgtttgg ttcaagttat gaagtagaat cttttccatt aattgagtat
     1561 tggtcttctc ttttcttaac aactggtggt caatagcgac acgtctcagc cagatattat
     1621 ctgataggct ccattttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
     1681 gattgctcaa aacactgttt tgaggttgca gatagagctg acgtggtttg aagagatttt
     1741 cgaagagtat taagattatt tcttctagtt cagggtgttc atacaccaaa ctccctacta
     1801 ctcgatctag gatatctacc gtgtcccacc aggattttgt cacgactaac tgctctagct
     1861 taggcaaatc ggtttccttt agataagact gcattgtttt caaatagtta gcagccacat
     1921 attggtattt tctaggctcc ttttcccagc aagtgtctgc aaaatcccaa tcgataatct
     1981 ttgttttttt cgcttctagt aaatattttt atagagttta tttctttcag gcaccgcaat
     2041 acctagaaaa gaaaattgat ggcgcatata ggcttccatg gaccttgctt ttttagagtc
     2101 ttttgctgct tctagctcct caagtaaatc tgctaaactc atctaaaact catcttgccc
     2161 caccaaatgg tgctgaaagg catagacagc cgcctgggta cgatcgctga cttcaagttt
     2221 ggcaagaatg ttggacacgt gggtcttgac cgtcttgaga gagataaaga ggtcatctgc
     2281 gatgcgctga ttttcgtagc ccttggcgat gagttggaga acatctcgct cacgcgcagt
     2341 caattcttca tgaagttcca tatgattgcg gtggtattca accttcttgc taacctcttg
     2401 ctcaatggcc agctcgccag cagctacctt actgacggca tgaagcaatt catctgcact
     2461 agaagtcttg agcatatagc ctttggcacc agcatctaag actggcatga ttttttcatt
     2521 gtccaaataa gaggtcacaa tcaaaatctt ggcttcaggc cattctttaa ggattgctaa
     2581 ggtcgcgtca atcccattca tctcaggcat gacaatatcc atgacaatga catctggacg
     2641 cagttccaag gccaagtcaa tcccttgaga cccgttggac gcctcaccca caacttctac
     2701 atcgtcttgg aggtcaaagt agcttttcaa acccaaacgg accatttcat ggtcatctac
     2761 tagtaaaatt ttcatcttta ctcctttatc attccttatc taacagggga atacggatat
     2821 caactgccag cccttgcttg ggagctgtca agagttgaac tgttccagcc atatcttcaa
     2881 ccctctcctt gatatttcgc agtccataac tcaagtcgtc taagcttcct aaccggaaac
     2941 caatcccatt gtccaccacc ttcagttgca attcaacatc tgtctgatag aggtagacat
     3001 ctaggcaaga tgcctgggca tggcggaggg tattgctaat caactcttgc aggatacgga
     3061 agatatgctc ctcgattttc ttaggcaatt tcgtcatatt ctgcttgaga ctaaccctaa
     3121 gatcactctt gtcctcaagc tcttttaaga gaatttgaat cccttctatc aagctcttct
     3181 gctccagttc aactggtcgc aaatgcaaga gcaaaacccg caaatccttc tgggcagttt
     3241 ctaaaatagc tgtgacactc tgcaactgga tctgcatctt ttctctatcc aatttcaaag
     3301 cctgctgact gatacccgat aaaatcatgt gggccgcaaa caactcctga ctgactgtat
     3361 cgtgcaaatc ccgagcaatt cgcttccgtt ctttctcgat gatttcctct tcctgagcaa
     3421 ggctatgatt ttcagctttt tgaagagctt ctgtcaaaag gttaagttta cctgataagg
     3481 acttgaaact ggcatccaaa tctggatctg caacctgaac cacttcttgc cctgccaata
     3541 aacgcttgag attagcctgc atttttctta gagaaagctc ttcgatccct cgccaaaaca
     3601 gggctaagag acaggttatg gacatgctga aaaccaacaa taaaaagaca aatttttctg
     3661 ttttttcgac atcgtgcaaa aagatagacc agtcaaaatc aagtatttcc agcaagctgt
     3721 gggagaaaaa aaagacaaac aggaaggagg tgagagcaat aatgacatag gcttgttttt
     3781 tcatcctcta accacctcca catcaccaat catagtggtc aagaaaatct tgacactctt
     3841 gttactcttg agatagtctt ttgtttcttg atgatagtgt tcattgcgga gggctcgctt
     3901 gggctggttg aaaaaagtca aatccccata gagacagtta acgctgagac tgacttccac
     3961 atctacaggt acgatgattt tggtcgttcc taccatcttt ctgaggataa tgacattgtc
     4021 atgattggtt aagatgaccc tctccagatg aatagtgtcc ttgcccatga agcgaaagag
     4081 attgatatca tcgaattggc aagtctggta gcttgaaaaa tgatgaagat ttccaaacca
     4141 acgatttttc tccttcttaa ccgtcacgac ctcttcaaaa accaaattgg tctgctcttt
     4201 ttcctggttc atcatcgggt aaagaagaaa gaggctatag ataaccgcaa caaaaatagc
     4261 tagaatcaca aaaggattga gcataacgat gaaaaagaag agaatggttg ccgctactaa
     4321 aagaagatta tttccctctt taccagtgta gtagcgaatc aaaagcaaaa agaggaatag
     4381 tatcagcaga aaacgcgaaa aatgctctga taccatcaaa atcagagctc ctgtcagaag
     4441 acaggcttcg ataaataaaa agattttaaa ttttctcata ggttcatcct ctcccttcta
     4501 ttttatcaca attcaaaaaa gtcacctcag tctgaggatg gaaaaaaggc gctggttacg
     4561 cctttttcat ctgatccttt gcttctttta attttccata aagaagatag tctacttttt
     4621 gtagatctgc tatggtggca cagttaaggg aacacataat caagcgtagg tctgctttcc
     4681 agccttggac aatgccaatc acttcttcaa ctgtgtaggt ttcaaccaat tccagaacgg
     4741 ttcgtgacaa tcccacagac ttagcaccaa aaaccaagca cttaatcata tccagcggat
     4801 tccgaacccc tccactaacc aagagttcga ccttatcttt ccattcttgg gcattgagaa
     4861 gggcctgcat ggtagactga ccccattgat tgaggtaatc acgctggcca ctacgacggt
     4921 tttcgatata ggcaaagctg gtgccaccac gacccgatag gtccactgta cgaacaccga
     4981 attcataggc tctttcgatt gtcttggcat ccattccaaa gcccacttcc ttgaggacaa
     5041 taggaacggg aatttgcttg ctataatctg ctagatgcga ttgccagctt ctaaacttcc
     5101 tttctccctc gggcatgagt aattcctgca tgacattgac atgcacttgc aatagaacag
     5161 gattcatctc ttctacagtc tgaagtccta actcgacagg tttgtccaat ccaatattgg
     5221 ttccaaggag gagattggga tgactagact tgacagaaaa agaatcatcc gttggatttt
     5281 tgagggctgc gctataagaa cccgttacaa ataaaatacc acaggattcc gccacctgag
     5341 ccagcttttg attgatttct cttcccttat tacttccacc agtcatggca ttgatataaa
     5401 aaggaaagtc ccactttcga ccagcaaact ctgtcgaaag atcgatttca tccagattgt
     5461 aaagaggcaa ggaagaatga atcagctcca cctcatcaaa gctattatag gaacttttct
     5521 gctcaagggc atagaggata tgctcgtcct tacgatttgt cgtcatgtcc tatcctttct
     5581 tgatataaga gctcaatccc cagatcggcc caacgatttt ttaaggtttc ggttgattgc
     5641 gcatcaaaac tcaaggcgat gccacagtca ccaccaccag caccactact cttggcaacg
     5701 gcctgcaaat cttgactggc ttctttcaac tgtctaagca aaggcgtgta aatatctgta
     5761 ctcaagcctt ctaaaagctt gctggctact tctacttgct cgataacttt ttcggctttc
     5821 ccctgctcca aggcttcgac caaagaaacc accgtttctt ttgaggaagt taaaaaattt
     5881 tgattgatat tttgcttgat ttgctggacc atgtgactcg atacagccac ttccttggtc
     5941 catcccacta agaaatcaca ttctaaagtt ggtttcactt gtgagataaa aaatccccaa
     6001 tcacgctcca gaactgtcgc caagttttct tcttctaacc aagcagccac cttctggcga
     6061 tcaaatgact ggtagagaac caaatcctct gccacaatac aggcaaggtc gcccatggaa
     6121 ccattgtctc ctcgcttgag caagacagcg ctagtcagct tgaacaagag attctgatca
     6181 accgaaagat tatagagagc cagtaaagcc ttgacaacca agacaacaac gctgccacta
     6241 gaacctagac caaacttttt cccttctcgt tccattttgc cacagatttt tagggaaaaa
     6301 gatcttaaat tctgaccgcg aacagcgagg aagtctccca tcaaagcaat cgtttcttga
     6361 atcaagctgt agtcaggatt aggccttaag tccactgcga aatcaaacat atctgaatag
     6421 atacggtagc tgtcagaaaa agcaatttca gccctcatat agatgggaat atcctttatc
     6481 aaagctaact gccctggctc taaaatagca tattcacctg cccaatagag ttttccgcaa
     6541 gttttaacag caatcatctt gactcaaatc ctttgttttt gacacaatca agcgataacg
     6601 ctgaccgaaa atttctgata aatgctccaa gtctttctcc tgacagaaga ccttaacatt
     6661 gggaccagca tccatggtaa agtagcaggc ctctcctttc tcacgaagct ggcgaacaaa
     6721 gtccatagcc tcataagagg catccgtcag ataagaaaag gctggactag cagtctttgt
     6781 cgtagcatgc atagccaggg catttttctc cgttaattct ccaatcttgg caaaatcatt
     6841 ttccttgaga taaatcagca tatcctgata gtccttctca gactgacgaa cccagtcgtc
     6901 gaaagtcgtc gaggtttcca cacaaagttt catcccgtca cggctagaga ttggtttttt
     6961 cttgtcctct agcaccaaca taatcatagc tagtttcaag tctgtctcta cagggtaaat
     7021 ttctccacta tccttatccc aggctcctag tggtccataa aaactccgag aagaagagcc
     7081 tgaggcaaat ttggcttcct gtgccaactg acttctatcc aatccaagtt tgaaataagc
     7141 attacaagcc ttgaccaggg cggacaaacc actagaactt gaggacaggc ccgctgccgt
     7201 aggcatattg ttttgagtat cgatacggac aaagccctca ccagctggac gataacggtc
     7261 aataatctta ctcatcttgg catgctcgac ctcattttgt agctgaccat tgatgtaaaa
     7321 ttcgtcagct gttacattgg ctggtaaagg cgacaaggtc gtctctgtat acatattttc
     7381 caaagttaga gaaatactgc tagtagcagg caccatctct ttttcttttt tctttcccca
     7441 atatttgata atagcaatat ttgcgtagga acgtactgtt acaggctctc tatccatgtc
     7501 tgaacagctc ctttctcttc taatctttct gctagttctt gtgcgtgtgt caaattggtt
     7561 accaaggcta tgatacaacc tcctagccca ccaccgctca tcttggcacc cagagcacca
     7621 tggctaagag tcgtttcaac caaaaagtct gcctcagggc tactgactcc aatttctttt
     7681 aaatgtaaat gcgcttgact gaggatttgt cccagtcctt cagcatcttt ttgtgaaatc
     7741 gcaacttctg cttgctgggt taattctccc aaggcatgca aaaacggtag ggcatccttg
     7801 cccttatttt gaaccacttg gatggcttca cgagtatgac cataaacacc cgtatcggca
     7861 atcaccaaat aggcggataa atccatctca agttctgtaa atcctacgtt cttgataaag
     7921 cgaataggtt ggtcactgag acaggtctta gcatccaaac cactaggatt catatgggca
     7981 atcatttcag ctcgattgac caagatttct agtacatcat gaggcagatc agcctgatag
     8041 tagtcaaata ccgcacgaat ggccgctatg ctgatagccg ctgacgaacc catcccccgt
     8101 ttctcaggga tagccgagtc aatctcacaa cgaatgcagg cttctgtgat atccaaatac
     8161 tccagtgagg cataaaccgc catggacaag gtatcctcct cataaaggcg ccaaggactc
     8221 tctgcaggaa ctaccttaca ggtcacctcc acctccaaaa gaggcaggga aatggcagga
     8281 taaccgtaaa cgaccgcatg ttcccctatt aaaattatct tactatgtgc ctgaccgaca
     8341 ccaacttttt ttgtcatttt ttccttttac tagacgaaaa aacgtcttat ttttcataca
     8401 agtattaatt ctttcctatc tattttatta tattttcaca aaaaaagcga ttgtttccat
     8461 tcacaatcgc ttctttcatt attgaaccca ttcgccatta tagttgacag aatagccatc
     8521 tacggtcgta ttcactgcca aggcacctga gctataagcg tagtaccatc tgccattgac
     8581 ctggaaccaa cctgtcgtca tagaacgacg aaagaaactc cataccatta agtaaagagg
     8641 aaagtcgtga gggagcatgc gccattgaca acttgtttta gtgacgtaca aagtctcatt
     8701 aacaagtact cgtttcggcc atttataggt gcggtgtttg gagaaatagg gttcaatctt
     8761 agcccattct tgatcgttta aatcagtatc atatgctttg cgtgtcataa ctctagttta
     8821 acattttttt gtgaatacag gttctaaata atcgaccacg aaaatttctt aagtggaaaa
     8881 cgccttatga agtatgctac gggaaagtta tgcacttaat ttgacaattc aagatgtaaa
     8941 aatata
//
LOCUS       DAVKLW010000075         8911 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKLW010000075 DAVKLW010000000
VERSION     DAVKLW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.075"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            81..533
                     /locus_tag="VW324_001851"
     CDS             81..533
                     /locus_tag="VW324_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9727419.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(568..1956)
                     /locus_tag="VW324_001852"
     CDS             complement(568..1956)
                     /locus_tag="VW324_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9727420.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2022..2984
                     /locus_tag="VW324_001853"
     CDS             2022..2984
                     /locus_tag="VW324_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9727421.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3190..4200)
                     /gene="ccpA"
                     /locus_tag="VW324_001854"
     CDS             complement(3190..4200)
                     /gene="ccpA"
                     /locus_tag="VW324_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9727422.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4625..5224)
                     /locus_tag="VW324_001855"
     CDS             complement(4625..5224)
                     /locus_tag="VW324_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9727423.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5226..6323)
                     /locus_tag="VW324_001856"
     CDS             complement(5226..6323)
                     /locus_tag="VW324_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9727424.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(6323..7060)
                     /locus_tag="VW324_001857"
     CDS             complement(6323..7060)
                     /locus_tag="VW324_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9727425.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(7062..7946)
                     /locus_tag="VW324_001858"
     CDS             complement(7062..7946)
                     /locus_tag="VW324_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727426.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(7933..8127)
                     /locus_tag="VW324_001859"
     CDS             complement(7933..8127)
                     /locus_tag="VW324_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727427.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(8124..8330)
                     /locus_tag="VW324_001860"
     CDS             complement(8124..8330)
                     /locus_tag="VW324_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727428.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         2742 a         2025 c         1562 g         2582 t
ORIGIN      
        1 tttgaaaaat actttttttg ctttacttct atagtgaaaa ggtttacaat tatagtaaga
       61 aaatttcagg aggtttcatt atggcacaac gttaccaaaa tatcatggtc gcaatcgatg
      121 gttctaagga agcggacttg gcttttgtca agggagttca ttctgctcta cgaaacgacg
      181 ctaaactcac catcgcacat gtcattgaca cacgcgctct ccaaagcgta tccacctttg
      241 atgctgaagt ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa gagtacgaaa
      301 aacgtgctaa agatgctggg gtagcagatg ttcatatcgt cattgaaatg ggaaatccaa
      361 agaccctgct agcacgtact attccagatg ccgaggaagt ggacctcatc ctcgttggcg
      421 caactggtct caacgccttt gaacgcctct tggtcggctc ttcatctgaa tacatactcc
      481 gccatgctaa ggtcgatttg ctggttgtga gagaacaaga aaaaacctta taatcacaaa
      541 gaaaaggagc ccctagctcc tttttgttta cgatttattt ctctctttat ggcgttcgta
      601 agccttgagc tggcgctgca gttccttttt aatagcaggt tctggagcat atttttcttc
      661 ccaattatct ggttttaaga ttttatgggt cactggatca aaatgagcct tgccatctgg
      721 aaaaattttc cccatattgg cctgatggac aatatcaaaa atacgttctg ggtccacccc
      781 catcaagaca aaactgccgt aggtgaagta aagcgtgtca atcaaggcat ccacttgccc
      841 tatcaaatct tgctgagcag gtgtcttctt ggctacttta cctgctgcct tatcaagggc
      901 ctgatgaagt tgcgatacag cttgaccaaa atcttcttca gaaggactgg ctgatcgaac
      961 aaactccacc aattcttcta ttttaaaacc agccctatgg gttgcaccct ctaaatccca
     1021 agctcgaggt tcttcttggg ttcgttcatc catcatgtgg tggaaggtct tgaccttatt
     1081 gaaatgatag tcacggctga caaaaacttt ttctgaagac aaaacaccaa aatgttccaa
     1141 ggccttgtgg atcccatctt gttgattgct agtggtaatg tgcttggcag cttccttgac
     1201 actgctacta ccatttccca tagcgaccga cataccgacg ccagccagca tttcaaggtc
     1261 attatctgag tcaccaaaag ccatgacttg gttgaggtca aagccatatt ctttcccaac
     1321 tcggcgaatg ccttctaatt tagaatttcc ctgattgatg acatccgatg caaaaggatt
     1381 gctacgtgtc aatttcaagt cttcaaaatc agctgccgcc ttctcagatt cttctggtgt
     1441 catcagcatc aaaacttggt aaataggttg attcatcagg tgaagcaggt cctcttcctt
     1501 ttggggaaca accttgctga ccatgcgatt aaaagactga ctcaccgtcc gagttaaaac
     1561 agagggaacg aagcgactaa ttcgttggga aaaagaaccc agaccaaagg acatgatttt
     1621 agaacccaac atggcatcct tggtccctaa ggcaatctcc gtgccctctt ttttagcata
     1681 gctaattaga tggcgcaaat gtaacttgga aatagggctc gtgaacaaga ctctgtcttt
     1741 actaaagata tactggccat tataggttac cgcaaaatcc agatccaaat cgtccatcaa
     1801 ttccttaaca aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc cttgttcttt
     1861 gacaatctta atcgcatcct tagtggattt caaaacactc ttgcgattgt tgaccaaggt
     1921 tccatcgata tcaaaaaaaa cagctttgac ttccatccta tcccaatctc cccttttgtg
     1981 atacaatgat tataccacat ttcagaaaga gtgagtaaat catgcctaag aaaatccttg
     2041 ttttacatac gggtggaact atttccatgc aggccgatgc ttctggcgct gttgtgacga
     2101 gttcagataa tcccatgaac catgtgtcca acccacttga aggaatccaa gtccacgcct
     2161 tggacttttt taaccttcca agtccccata tcaaacccaa acatatgctg gtcctctacc
     2221 agaaaattaa agaggaagca gataactacg atggagtggt gatcacacac ggaaccgata
     2281 ctttagagga aacagcctat ttccttgata ccatggaagt tccccatatg cctatcgttc
     2341 taacaggagc catgcgtagc tccaatgaac tcggtagtga tggtgtttat aattacctaa
     2401 gtgctttacg agtggccagc gatgacaggg ctgctgacaa aggagttttg gtcgttatga
     2461 acgatgaaat ccacgctgcc aagtatgtca ccaaaacaca tacgactaat gtcagcacct
     2521 tccagactcc aacacatggc ccccttggtc tcatcatgaa acaggaaatc ctctacttca
     2581 aaacagctga acctcgtgtt cgctttgacc ttgatcacat acaaggttta gtccctatca
     2641 tctcggctta tgctggtatg acagatgagc tgattgatat gctggattta gaacacttgg
     2701 acggtttgat tatccaagcc ttcggagctg gtaatattcc caaagaaacg gctcaaaaat
     2761 tagaaagcct tctgcaaaaa ggaattccag tcgctctggt atcacgatgc tttaacggta
     2821 ttgccgagcc tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa gcaggcgttt
     2881 tctttgttaa agaactcaac gcccaaaaag ctcgcttgaa actcctcatc gccctcaatg
     2941 ccggactaac aggacaggct ttgaaagact atatggaagg ctaatactct tcgaaaatct
     3001 ctgcaaacca cgtcacgtcg ccttaccgta tgtatggtta ctgacttcgt cagtttcatc
     3061 tacaacctca aaacagtgtg ttgcgcagcc tttaaccagc cgcctagtcc gctctatggt
     3121 attcattaag tcaacatctc ttgtttaaga gcacaaaatc aggaaatctt ctcgattccc
     3181 tgattttttc tatttacgtt ttcgtgttga gctacgttct gtcaaaccat gaggtaagag
     3241 aacttcacgt tcttccaact cttccttatg cataatcttg gtcaacatac gcatactaat
     3301 ggcaccaagg tcataaagag gttgggcaat cgttgtcaag tttggacggg taaagcgtga
     3361 gatttgtgaa tcatcactag taataatttc aaaatcttct ggcacagaaa cacccttatc
     3421 agccaaaccg ttcaagactc ctgctgccaa ctcatcacct gtcacaactg ctgcagttgc
     3481 atttgatgaa atcaaacgct ctgctaaggc gtaaccatca tcatagctat atttagattc
     3541 aaataccaaa ccctcactat aagtgattcc tgcttttttc aaggtttcct tgtagccaac
     3601 taaacgaacc ttaccattga tgtcatccac tagcggaccg ctaacgaaag caatacgctc
     3661 attttcttta gcaaggtaac tcactgcatc aattgttgct tgcttatagt caatattgac
     3721 acttggcaac tggtgctcaa catcgacagt tcctgcgaga acaatcggag tacgtgaacg
     3781 cgaaaattct gagcgaattt tatctgtcaa gtgatacccc atatagataa tgccatctac
     3841 ctgctttgaa aagagggtat tgacaacaga aacttctttc tcgttatctt catcgctatt
     3901 agctaggaca atattgtact tgtacatttc tgcaatatca tcaatcccct tagccaaact
     3961 cgaaaaataa ccattggtaa tatttggaat cacgacaccg acagtggttg tctttttact
     4021 tgcaagacca cgcgcaactg catttggacg ataatccaaa cgatcaatta cctctagcac
     4081 ttttttacgg gtattctctt ttacattttt attgccattg accacacggc tgaccgtcgc
     4141 catggaaaca cctgcttcac gagcgacatc ataaatggtt actgtatcat ctgcattcat
     4201 tccttttcct gtcctttcta tctcacacat tcttttacaa gtagaggtac tgattgaagc
     4261 tctatatcta cttacaaaag tgaagatgtg aaaatttcgt tttcatattt ctacttattc
     4321 cattctatca ctaattgtaa acactttcaa gtgttttttg aagattgatt gaaaaaattt
     4381 catagaaaac ctaggtttag ctccttgcta ccaccttaga ctaaacaaaa aggaggaaac
     4441 taagccctcc taaagttata gtaaaatgaa ataagaacag gataaatcga tcaggacagt
     4501 caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatctact
     4561 ataggtattg ttccattcac taccgtcaat tttagcacat agtcttcatg aaaatattat
     4621 atcatcataa ccaaccagat tcttttgcga tattagctgc ctctgttcga ttaccagcat
     4681 ccagttttga aagaatattg gtgacatagt ttcgaatagt tccgtttgag aggtaaagct
     4741 gatctgcgat ttcttggttg gataagcccc gggcgattcc ctttaagaca gcaatctctt
     4801 gttctgtcaa cggattggga cgggtcatca ccatttccat caattcaggc gaatactcct
     4861 tgcgtccttc gaggacggtg tgcaaggttt gcatgaggtc tgcaatgctt cgttccttta
     4921 atacataagc atccactcca gccttgaccg cacgttcaaa atacccagca cgcttgaagg
     4981 tcgtcaccac aaccaccttt gtttcaagct tttctgatcg tatccactcc aagacttcaa
     5041 gacctgtctt aacaggcatt tctacgtcaa ggatggcgat atctacagac tccttttcta
     5101 atagttggat tgcttcttgc ccattcttgg cttgaaggac agactccaca tctggttgaa
     5161 gcgtgagcaa ttgacacatg gcatctcgca acatactttg atcttctgcg actaatactt
     5221 tcatcttagt ttctctcctt ataaggtagt cgaacttgca cttccgttgg atgtttctga
     5281 ctgattacac ttacttctcc tgaaaatgga aaaacacgat ctcggactgt atggagctca
     5341 tctcctttta gaaaagcaaa gccgcagcca tcatcactta ctgttaaaat gagttccttc
     5401 tctgtccgtt ctaattttaa gtaagcttta gacgctttgg catgtttgat gatattggtc
     5461 accaactcaa gcaaaatcat agaagccatt gactccaatt cttgagtaag gctagcagta
     5521 tctagttggt tatccgtttc cacctcaatt ccagcaattt ctaacatctt tttcacagtc
     5581 tctagttcgg atgtcaaagt tctagactta agattttcca cgatagttcg aacttcattc
     5641 atggaatcct tgctgatctg gtgaatttct tttaattcct tttccacctg tggataagcc
     5701 tccatctgaa ataactgcaa ggctagatca gttttaacac taagcatagc aaaggtatgc
     5761 cccagactat catgcaaatc ttgaccgata cgattgcgtt cattttcagc aagcaataga
     5821 tttatctggg cattttgctt agcctgtgct tctttcaaat cctccacaat tcgaatccga
     5881 accaagccaa aagtcattaa atcgacaaaa gcaagaatta caagtagata gaatagaaac
     5941 tcaacttcga ttctctgaaa aatcaacagt tgacccacaa caaggacttg agcaagaaga
     6001 aaagtccaga catgtaaaga ctttaaacca cctacgctga aatgatagct taagagattg
     6061 gataggaaaa agaaaaacca gatataatta acagccacaa aggcagtatt cccaactaca
     6121 taagtcagca ggatgcccca atatagccaa gatagacgct ggctcttagt tgttaaaaca
     6181 cccaaatacg ccactacaaa tagaatatca atcaataaat gccaggcaga aagccaccca
     6241 gtcactacag ttaggatggg gaaaaccata aaaattaaac tgatccaaaa catataatgt
     6301 atgcttttca gtctttcaag cattaagcat tctccttatg accttgaagg taaatggtca
     6361 aaccaaacaa aactactgaa aaaacaagta aataaactgt ggctgataga ttgatgccac
     6421 cctcatttaa gaaggtcttg agcaactcca tcaactgata ggtcgggaga cacttaccta
     6481 ctacttgcat ccagtctgga aataaagaga taggcatcca gagtccacct aaaacagcca
     6541 accctagata aagaagattg cccacgacag acatcaactg actagttggt aagagagtca
     6601 aggtcaaacc aagcgctacg aaggcaatac ttcctactat cagcaaaagt gcagccccaa
     6661 tccaatttcc aagagacatg tccacacctc ttacaaaatg cccaactgag aaaaccacca
     6721 agattgaaac caaataatca accagcatac ttgttatctt tgatagataa tattctacca
     6781 tatttacagg gctatgacgc aatgttttct gccagttgtt gatcttgtcg gtatgtaaaa
     6841 caactgggaa tgagaagata gctgttgaca tcatggaaaa tgcagtcatg gagataagat
     6901 aatcacgcat aaaattcgcg agttcacctg gtgtgtcctg atagatacca gaaaaaaata
     6961 aatagaaagc cgtcggcatc cctaccgaca atagataata gattaattgt cgtttggtca
     7021 ataaaaattc tatcttacta agtgctatcc atcgtttcat cttagttatc tcccttctgc
     7081 gtctcttcaa agattgtatt caacaaacta cgattattaa cttcgatttc ttgcatccta
     7141 catcctgctt gggctaatag ttcccagaaa gcatctgctt ctcgagtgac tacttgtaaa
     7201 gaatcttgct ttagggtcca gttttcaacc aaatttgact gctctacgac ttccttgtaa
     7261 gctataggaa ggataaagtg cttttctatt tcctcgctac gcatagctag aggcgtcgta
     7321 tctcgaatca actctccctt atttaagagc aaaatgcggt ctgcagtgtg ttccacttcc
     7381 tcaatataat gggacgaata gagaatggtg actccttgcg cttttaactc ctggacaatt
     7441 tcccaaaaac gttgacgagt tgaggtatcc atggacgcag taggctcatc taaaaaaaca
     7501 atctttggtc gcccaattaa ggtcaagatg aaagaaaaga gacgcttttg accgcctgat
     7561 aatttttctg ccaactgttc tttttgttgc ttgacaaact gcaatagttg atcaatttcc
     7621 tgattgctca aaggatttgg atagatacgt tgaaagaaag caatcaactc tttaactttt
     7681 aatttctgaa cgattatatt ttcttgaggg aggtaagccc tagtatagtc taacttagaa
     7741 ctcgtcactg gtaaaccttg aatagatact tgaccgcttg tgaccagttt atctccaagc
     7801 aaacaatcta agagtgttgt ctttccggca ccatttggac caattaaggc aacacattca
     7861 ccatcgttga tttcaaagga gatgttccgc aaaatttcct tgtcttttat tttcttactc
     7921 aatttttcaa ctttaaccac agtcatgaga ctctcctttc aaccactcca ttcccatagg
     7981 gaaaacgacg aaaatcataa atccaaaccc caaagcacca caaatgaatt ggcgaagcaa
     8041 ggcttgatca aaccaatcag taaacatttc cactaaccat accaagagtg acaggccgat
     8101 aaagaaatag atgatccctc tcttcattcc tcaaactcct ttttcacatc tctgactaat
     8161 ttcaaacctt ctctaacaag ccaagacatg attccaaagc cagcaaagac cttccaagga
     8221 aaatggtaga aatcctcatc caatcccgaa aacatgagat aggtcataac tcctgctact
     8281 actaaactca ttgcaataat cattttattt ctcatctctt cttcccccat ttcatactac
     8341 aattatagtc ttttaaaatc agaggaggca gaagcttctg tcactagaaa atatgacaaa
     8401 tgtcataaaa attctgttca aaacaagcaa gatacactat acaataaaac acaatcagaa
     8461 aaatctaagg caacttcctc aaaagagata tcaaacccaa ttcacaccat aatgtaaact
     8521 aatacttatt taaaatcaaa aagagtagaa atttttatca gacaaacaca tatatagtgt
     8581 attgaatcta taacagtagg ccttaaatac taaaatattt ctataaatta atttaacttt
     8641 cctgatagag ctgttcatat cttatttcaa ttctctatat tatacgttga acaaaaccct
     8701 tctattgctt tcttaaaaat ttataagagt tataaaatct gttaaatttc aatgtgtata
     8761 cctaaactac ggtatttatt gaaaagactg gagacaaaaa gtatacgctg ccaaaatgaa
     8821 ttactgaaaa tcaaaaaaga gagaaccaaa ctgattccct cttaatgtat ataatatcta
     8881 gttttaaaaa tacacactca catatctctg t
//
LOCUS       DAVKLW010000076         8826 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKLW010000076 DAVKLW010000000
VERSION     DAVKLW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.076"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..582
                     /locus_tag="VW324_001861"
     CDS             <1..582
                     /locus_tag="VW324_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9727429.1"
                     /translation="NAEKLQGKGTKNKIVSKSLTQLDFVFKNNEEKDHVALLGRIGSE
                     RVYRYINKKYLDLPETFENYNVFVPEANGSGALGEVLSTPLIGEPLIGEPLIGHTDTF
                     LSIGNFKTKFEADACIKFIKTKFARVLLGVLKVTQHNSRKTWYYVPLQDFTVNSDIDW
                     TQSVADIDLQLDQKYDLSPEEIAFIENHVREMD"
     gene            747..4001
                     /locus_tag="VW324_001862"
     CDS             747..4001
                     /locus_tag="VW324_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9727430.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(4387..5373)
                     /locus_tag="VW324_001863"
     CDS             complement(4387..5373)
                     /locus_tag="VW324_001863"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9727431.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            5567..8035
                     /gene="gyrA"
                     /locus_tag="VW324_001864"
     CDS             5567..8035
                     /gene="gyrA"
                     /locus_tag="VW324_001864"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9727432.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            8035..8778
                     /gene="srtA"
                     /locus_tag="VW324_001865"
     CDS             8035..8778
                     /gene="srtA"
                     /locus_tag="VW324_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9727433.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         2858 a         1512 c         1881 g         2575 t
ORIGIN      
        1 aatgctgaaa agctgcaagg aaaagggaca aaaaataaaa ttgtttcgaa atcactaact
       61 cagttagatt ttgttttcaa aaacaacgaa gaaaaagacc atgttgctct acttggaaga
      121 attggctccg aacgtgttta tcgatatatt aataaaaaat atttagattt accggaaaca
      181 ttcgaaaatt ataatgtttt tgtaccagaa gctaatggaa gtggtgcctt aggtgaagtc
      241 ttatcaacac ccctaatcgg ggaaccccta atcggggaac ccctaatcgg gcatacagat
      301 acttttttat ctattggtaa ttttaaaaca aaatttgaag ccgatgcttg tattaaattt
      361 attaaaacta aattcgctag agtattatta ggtgttttga aagttactca gcataattca
      421 cgcaaaactt ggtattacgt ccccctccaa gactttacgg tcaattcgga cattgattgg
      481 acacaatcag tggctgatat tgacctccag cttgatcaaa aatatgacct ttcccctgaa
      541 gaaattgcct ttattgagaa tcatgtaagg gagatggatt agaaaagtat ttttatttga
      601 caaatagtgc tcaatgatct aaaatgacta tataggatta ggtcaggaag catacgatgc
      661 cctgaccctt tttgtactta tgagatgaga aagtcatttg ttagataaat tgacttcgtt
      721 agcaaacgtt caaaaaagga aaacttatgc cagtagaaat taaaaccact aaagagattc
      781 atcctaaaat ctatgcctat accacaccga cagtaaccag taatgaaggc tggattaaga
      841 ttgggtatac agaacgtgat gtcacacaac gtatcaagga gcaaacgcat acagctcata
      901 tagctacaga tgtcttatgg actggtgatg cagcttatac agaagagcct gataagggga
      961 aaactttcaa ggaccatgat ttccaccatt tcctttcttt ccatgatgta gaacgtcgtc
     1021 ccaagacgga atggttctat tttaatggaa ctcctgaaaa atcaaaaaat ctttttgata
     1081 agtttgttca gcatgatttg tctggttatc agcctggaaa aggacaggac tatactctgc
     1141 gacaagagca agaagaagca gttgctaaga cattagctta tttccaagaa catgctggag
     1201 gcaagtttct ctggaatgcc aagccacgct ttggtaaaac cttgtctacc tatgacctag
     1261 ctcgacggat ggaagctgtc aatgtcctaa ttgtaacaaa ccgccctgcc attgctaact
     1321 catggtatga tgattttgaa acattcatag caggtcaaac gacttacaag tttgtttctg
     1381 aatcagatag ccttaagagt cgtccaatct tgtcacgaca agaatttctt ggtattttag
     1441 ctgacgatgt aagacaactt gcttttatca gtctccaaga cttgaaagga tctgtttatt
     1501 taggtggaga gcacgataaa ctcaaatggg taactgatct gcattgggac ttgttggtta
     1561 ttgacgaggc tcatgaagga gttgatacct tcaagactga ccaagccttt aataagattc
     1621 gacggaattt tactctgcat ttgtcaggta catcatttaa agcattggct aaaggagatt
     1681 ttacagagga acaaatctac aactggtctt atgctgatga gcaggctgct aagtattcgt
     1741 ggtctcttga gcaagaagag gaaaatcctt atgaaagctt gcctcagttg aatctcttta
     1801 cctatcaaat gtctcagatg attggcgaaa agttagaaaa aggcgctcag atcgatggtg
     1861 aaaatattga ctatgttttt gacttaagtg aatttttcgc tacagatgat aaagggaaat
     1921 ttattcatga gcatgatgtc agaaattggt tagatactct atcaagcaat gaaaaatatc
     1981 cattttcaac caaagaactc cgtaatgaac tcaagcatac tttttggctt ttagaacgtg
     2041 tcgcttcggc caaagcatta aaagccctac tagaagaaca cccaatctat gaaaactatg
     2101 agatcgttct agctgctggt gacggacgta tgtccgaaga agacgataaa gtcaaactca
     2161 aatccttgga cttggttaga aaagcgatag cagagaatga caaaaccatt accctatccg
     2221 ttggtcagct gacgacaggt gtcactatcc ctgaatggac aggtgtattg atgttatcaa
     2281 atttgaaatc accagctctt tatatgcagg ccgccttccg tgctcaaaat ccttactcat
     2341 ggagcgataa caaaggaaat cactttcgca aagaaagagc ctatgtattt gactttgcgc
     2401 cggaaagaac cttgattctc tttgatgagt ttgccaacaa cttatcgctt gtaactgcag
     2461 ctggtagagg aacttcagct acacgcgaag aaaatattag agaattatta aacttctttc
     2521 caattattgc cgaagaccgt gctggtaaga tggttgaaat tgatgcaaag gcagttctaa
     2581 ccactcctcg ccagataaaa gctagagaag ttcttaaacg aggttttatg tccaatctct
     2641 tatttgataa tattagtggt attttccaag caagtcaaac agttttagat attttaaatg
     2701 agctgccagt tgaaaaggaa gggaaggtac aagatagttc tgatttatta gatttttcag
     2761 atgttacagt cgatgatgag ggaaatgcag tagtagacca tgaaattgta gttaatcagc
     2821 aaatgcgact ttttggtgaa aaagtttatg gacttggtga atctgttgct gagttagtca
     2881 caaaagatga ggaacgaact caaaaacagc tggtcaatga cttgagtaag accgtttctt
     2941 cagtgattgt agaggaattg aaagcagatt attctctaaa aacaagggaa actgagcaaa
     3001 ttaagaaaca aattacagca acacttgaga atgaaattcg aaaaaatgat atcgaaagaa
     3061 aaatttctga agctcatatc aagcaagagt tgcaacagca gctcaaagaa gcaaatgata
     3121 aagcgcaaaa agataagatt caagaagatt tagaaaaacg tttagaagaa aataaactca
     3181 ttcataaaga aaaactagaa caaacactca aaaaagaagt ggaaaaaatg cctgagaaat
     3241 ttatcgaaca ggttgagata aaacgtgtgg aacagttgaa acaatcagct caagatgaaa
     3301 ttcgtgacca tttacgaggg tttgcaagaa caattccaag ttttattatg gcttacggtg
     3361 atcaaactct aacacttgat aattttgatg cctttgttcc tgaacatgtt ttttatgaag
     3421 taacagggat tacgattgat cagtttagat atttgcgaga tggtgggcag gattttgcag
     3481 ggcatctctt tgataaagca acatttgacg aagctattca agaatttctt cgcaagaaaa
     3541 aggagttggc ggattatttt aaagatcaaa aagaagacat ttttgactat attccaccgc
     3601 agaagaccaa ccaaattttc actcctaaac gagtggtgaa aaggatggta gatgatttgg
     3661 aaaaggaaaa tccagggatt tttgatgatc catctaagac ttttattgat ttatatatga
     3721 agtcaggcct ctatattgca gaacttgtga agcggttata taatagcaat ggcttgaaag
     3781 aggcctttcc aaatcctgaa gaacgcttaa aacatatttt ggaaaagcaa gtttatggat
     3841 ttgctccgtc tgagattatc tataacattt ccactaattt tatatttggt aatctttcta
     3901 aagatatcag taggaagaat tttgttttag cagataccat tccagcggct aaagaaggga
     3961 gcattcaaaa gttggttgat tcctattttg aaaataatta aaaagaaggc cgagtcaaaa
     4021 ttctttgaaa tcagaaaaaa cgcataatat tgagtgcttt tgtactgccc ccaaaaagtt
     4081 agacagaaaa aatctaactt ttggggggca gttcagacaa tccttggtat tatgcgtttt
     4141 attgtgggaa gatgtataat ggattgaaat aagatatgaa caaatcgatt aggaaaggaa
     4201 aattgattta tagaaatgtt ttagtagtcg atgcgtactg ttatagattc aacgaactat
     4261 aaaatctgaa gaaagcaatt ttaaccagat cttagaagca gttgtgtact actctaactt
     4321 caatagacta tatcatatag ataaaaaaca actccctgat gattcaagga gttgtctata
     4381 gttaaattag tttttagaag cttcttggaa ttctggattt ttccatgctt cgtcaatgat
     4441 agcttgcaat tctttagcag atgcttgcat tttttgagtt tctgcatcgt tcaatggaat
     4501 gtttactgga cgaacgatac catgtgcacc aacaacagct ggttgaccga taaagacatt
     4561 ctcaactccg tattgacctt cttggaatac tgaaagtgga agtactgcgt tttcatcgtc
     4621 aaggattgct ttagtgatac gagcaagggc tactgcgata ccgtagtatg ttgcaccttt
     4681 tttgttgatg attgtgtagg ctgcatcacg aacaccttcg aacaattcaa tcaattcagc
     4741 ttcttgaaca ttttgagtgt ctttaaggaa ttcttcaagg tttacaccag cgatgttagc
     4801 gtgtgaccaa acagcgaact cagagtcacc gtgttcaccc atgatgtagg cgtgcactga
     4861 acgagcatcc acatccaatt tttcagcaag tgcttgacgg aaacgagctg agtcaagtga
     4921 agtacctgaa ccgataacgc gttctttagg gaaaccagag aatttccaag ttgagtaagt
     4981 caaaacgtca actgggttag cagcaacaag gaagatacct ttgaaaccag attcaacaac
     5041 ttgagttacg attgatttgt tgatagcaag gtttttacct acaaggtcaa gacgagtttc
     5101 acctggtttt tgaggtgcac ctgcagtgat cacaacaagg tcagcgtctg cacagtcaga
     5161 gtattgagct gcatagattt ttttaggtga agtgaaggca agggcgtgac taaggtcaag
     5221 cgcatcacca acagcttttt catgcaattg tggaatttcg ataattccaa gctcttgtgc
     5281 aattccttgg ttaacaagtg caaaagcgta agatgaacct acagcaccat caccgacaag
     5341 gataactttt ttgtgttgtt tagttgaagt cattgtttta aacatctcct taattttatt
     5401 aggggatttt ccctagacaa cttcattcta tcacttttaa aaaactttgt cacgaatatg
     5461 ccttatagtt ctcgatgtaa acgttttagt ggtttagagg ctgaaataga tgggaattta
     5521 tggtataatg ttgttactta ctaattgtga aatgaggcat ttattaatgc aggataaaaa
     5581 tttagtgaat gtcaatctga caaaggagat gaaggcaagt tttatcgact acgccatgag
     5641 tgttatcgta gcgcgagctc ttcctgatgt tcgagatggc ttaaaacctg ttcaccgtcg
     5701 cattctctac ggaatgaatg aattgggtgt gaccccagac aaaccccata aaaaatctgc
     5761 tcgtattaca ggggatgtca tgggtaaata tcacccacac ggggattcct ctatttatga
     5821 agccatggtc cgtatggctc aatggtggag ctaccgttac atgcttgtag atggtcatgg
     5881 gaattttggt tccatggatg gagatagtgc tgccgctcaa cgttataccg aggcacgtat
     5941 gagcaagatt gctctggaaa tgcttcgtga tatcaacaaa aatacagttg atttcgttga
     6001 taactatgat gccaatgaac gggaaccctt ggtcttgcca gcgcgttttc caaacctttt
     6061 ggttaatgga gcaactggta tcgcggttgg gatggcaacc aatattccac ctcataatct
     6121 gggtgaaacc attgatgcag tgaagttggt catggataat cctgaagtga ctaccaagga
     6181 cttgatggaa gtcttgcctg gaccagattt tccaactggt gctcttgtca tggggaaatc
     6241 aggtatccat aaggcttatg aaacaggtaa aggttcgatt gtcctacgtt ctcgtacaga
     6301 gattgaaacg actaagactg gtcgtgagcg tatcgttgta acagaatttc cttacatggt
     6361 caataaaacc aaggtgcatg agcatattgt tcgcttggtt caggaaaaac gcattgaggg
     6421 tatcacagca gtacgtgatg agtcaaaccg tgaaggtgtt cgatttgtta ttgaagtcaa
     6481 gcgcgacgcc tcagccaatg ttattctcaa taacctcttc aaaatgaccc aaatgcaaac
     6541 caattttggt ttcaatatgc tcgctatcca aaatggtata ccgaaaattt tgtctcttcg
     6601 tcagattttg gatgcttata tcgagcacca aaaagaagtg gttgttcgtc gtacacgttt
     6661 tgataaggaa aaagcggaag cgcgcgctca tatcttagaa ggtctcttga ttgcgctaga
     6721 ccatatcgac gaagtgattc gtatcatccg tgctagtgaa acggatgcgg aagctcaagc
     6781 tgagttgatg agcaagttta agctttctga acgtcaaagt caagctatcc ttgatatgcg
     6841 tcttcgtcgt ttgacaggtt tggaacgcga taagattcaa tctgagtatg atgacctctt
     6901 ggctctgatt gcggatttag cagatattct tgctaagcct gaacgtgttt ctcaaattat
     6961 caaagacgaa ttggatgaag ttaaacgtaa attttctgat aaacgccgta cagagttgat
     7021 ggttggacag atcttgagtc tcgaggatga ggacttgatt gaagaatcgg atgtcttgat
     7081 taccctttct aacagaggct acattaagcg tttggatcag gacgagttca ctgctcaaaa
     7141 acgtgggggt cgtggtgtcc aaggaacggg agtgaaagat gatgactttg ttcgtgagtt
     7201 agtgtcaact agcacccatg atcatctgct cttcttcaca aacaagggac gtgtctatcg
     7261 tcttaaaggt tatgaaattc ctgagtatgg tcggactgcc aaagggctac cagtagtcaa
     7321 tctcttgaaa ttggatgaag atgaaagtat tcagacggtt atcaatgttg agtctgatcg
     7381 cagtgatgat gcttatctct tctttacaac ccgtcacggt attgtgaaga gaactagtgt
     7441 taaggagttt gccaatattc gtcaaaatgg tctcaaagcg ctgaatttaa aggatgaaga
     7501 tgagttaatc aatgtcttgt tgacagaagg agatatggat attatcattg gtaccaagtt
     7561 tggttatgca gttcgcttta atcaatcagc cgttcgtggt atgagccgta tcgccactgg
     7621 tgtgaaaggt gttaaccttc gtgaaggaga cacagttgtt ggtgccagct tgattactga
     7681 tcaagatgag gttcttatta tcacagaaaa aggatatggt aagcgtacag tcgctactga
     7741 atacccaaca aaaggtcgtg gtggtaaggg aatgcagaca gctaaaatta ccgaaaaaaa
     7801 tggcttgctg gccggtctta tgactgttca aggtgatgag gatttgatga ttatcactga
     7861 tacaggtgtc atgattcgaa ccaatcttgc caatatttca caaacaggac gtgcaactat
     7921 gggagttaaa gtaatgcgcc tggatcaaga tgctcagata gtgactttca caacggttgc
     7981 ggtggcagaa aaagaagaag ttgggacaga aaacgaaaca gaaggtgaag cataatgtct
     8041 caaaaaaata ataaaaagaa aaacaagcga aaaaatctgc tgacaaatat cctagcagga
     8101 tttctgatat tactgtcact ggctttgatt tttaatactc aaattcgaaa tattttcata
     8161 gtctggaata ccaataagta tcaagttagc caggtatcaa aagaaaaatt agaagaaaat
     8221 caggatacag aaggcaattt tgactttgat tctgtcaaag ctatctcttc ggaagctgtt
     8281 ctaacttctc aatgggatgc tcaaaaatta ccagttattg ggggaattgc aattcctgaa
     8341 ttggaaatga atttgccgat ttttaaagga cttgataatg ttaatctctt ctacggagct
     8401 ggtacaatga aacgcgagca agtaatggga gaaggaaatt atagtctagc tagtcaccat
     8461 atctttggtg ttgataatgc taataaaatg ttattttctc ctttagataa tgctaaaaat
     8521 ggcatgaaga tttatctaac cgataaaaat aaagtttata cttatgaaat acgtgaagtc
     8581 aaacgtgtga caccggatcg tgttgatgaa gttgatgata gagatggggt caatgaaatc
     8641 acattagtaa cctgtgaaga ccttgctgct acagaacgta ttattgtcaa aggtgatttg
     8701 aaagaaacaa aagattattc acaaacatct gatgaaatcc taacagcttt caatcaacca
     8761 tataaacaat tttattaata caaatcagtg aaatcatgga tttcactgat tttttaagat
     8821 tttcat
//
LOCUS       DAVKLW010000077         8679 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKLW010000077 DAVKLW010000000
VERSION     DAVKLW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.077"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            153..2786
                     /gene="polA"
                     /locus_tag="VW324_001866"
     CDS             153..2786
                     /gene="polA"
                     /locus_tag="VW324_001866"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9727434.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2871..3308
                     /locus_tag="VW324_001867"
     CDS             2871..3308
                     /locus_tag="VW324_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9727435.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(3591..4601)
                     /locus_tag="VW324_001868"
     CDS             complement(3591..4601)
                     /locus_tag="VW324_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEU9727436.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            4750..5919
                     /locus_tag="VW324_001869"
     CDS             4750..5919
                     /locus_tag="VW324_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9727437.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            5916..6686
                     /gene="recO"
                     /locus_tag="VW324_001870"
     CDS             5916..6686
                     /gene="recO"
                     /locus_tag="VW324_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9727438.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDDRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            6683..7675
                     /gene="plsX"
                     /locus_tag="VW324_001871"
     CDS             6683..7675
                     /gene="plsX"
                     /locus_tag="VW324_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9727439.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            7681..7914
                     /locus_tag="VW324_001872"
     CDS             7681..7914
                     /locus_tag="VW324_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9727440.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            7960..8250
                     /locus_tag="VW324_001873"
                     /pseudo
     CDS             7960..8250
                     /locus_tag="VW324_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8453..>8679
                     /gene="blpU"
                     /locus_tag="VW324_001874"
     CDS             8453..>8679
                     /gene="blpU"
                     /locus_tag="VW324_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9727441.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCW"
BASE COUNT         2515 a         1573 c         2196 g         2395 t
ORIGIN      
        1 ttatagattc aatatattat attatagcac atttttaaat ctaggtttac taaattctct
       61 ggctctatct atttattcgg aaaaagtttg aaaaatactt gctctagctc ttcccaatgg
      121 tattttttga ttctttcctt tataatgggt gtatggataa gaaaaaatta ttattgattg
      181 atgggtcttc tgtagctttt cgggcgtttt ttgcgctgta tcagcagttg gaccgtttta
      241 agaatgcggc tggtttgcat accaatgcga tttatggttt tcagttgatg ttgagtcatt
      301 tgttggagcg ggttgagccg agtcatattt tggtggcttt tgatgcggga aagacgacct
      361 tccggacaga gatgtatgcg gactataagg gtggtcgggc caagactcct gatgagtttc
      421 gtgagcaatt tcctttcatt cgtgagttgc tggatcatat ggggattcgt cactatgagc
      481 tggctcagta tgaggcggat gacatcattg ggacgctgga taagctagca gagcaggatg
      541 gttttgatat taccattgtc agtggggaca aggatttgat tcagctgacg gatgagcata
      601 cggtggttga aatttccaag aaaggtgtgg ctgagtttga ggcctttacg ccagattacc
      661 tcatggaaga aatgggcctc acaccagctc agtttatcga tctcaaggcg ctcatgggtg
      721 ataagtcgga taatatccct ggggtgacca aagtcggtga aaagacgggt attaagctct
      781 tgctggagta tggttcgctt gaggggattt atgaaaatat tgatggaatg aagacttcta
      841 agatgaagga aaatctcatc aatgacaagg aacaggcctt tttgtctaaa acactagcga
      901 ccattgatac caaggcaccg attgcgattg gtttagagga cttggtctat agtggtccag
      961 atgttgaaaa tcttgggaaa ttttacgatg agatgggatt caaacagctc aagcaggctt
     1021 taaatgtgtc gtcagctgat gtggctaagg gtttggattt tactattgtt gaccaaatca
     1081 gtcaagatat gctgagtgaa gagtctatct tccactttga gctttttggt gagaattacc
     1141 atacggataa tttggttgga tttgcctggt cttgtgagga taagctctat gccacagaca
     1201 agcttgagct gttgcaagac ccgattttca aggatttctt agaaaaaaca tctctgagag
     1261 tttatgactt taagaaggtt aaagttcttt tgcaacgttt tggtgtggat ttgcaggcgc
     1321 ctgcttttga catccgtttg gctaaatacc tcctttcgac tgtggaggac aatgaaattg
     1381 cgaccatcgc tagtctttat ggtcagactt acttggttga tgatgaaact ttctacggta
     1441 agggtgttaa aaaggccatt cctgaacgtg agaaattctt ggaacactta gcttgtaaac
     1501 ttgctgtttt ggtagaaaca gagcctattt tacttgaaaa actcagcgaa aatgggcaat
     1561 tagagcttct ttatgatatg gagcaacctc tggcttttgt tcttgccaag atggaaattg
     1621 ctgggattgt ggtcaagaaa gagaccttgc ttgagatgca ggctgaaaat gagcttgtca
     1681 ttgaaaaact gactcaagag atttacgagc tggctggtga ggagtttaat gtcaactcgc
     1741 ctaagcagtt gggcgtgctt ctctttgaga aattgggact tcctctagaa tacactaaga
     1801 aaaccaagac aggttattcg acagcagtgg atgtgttgga gcgtctcgct cctattgctc
     1861 cgattgttaa gaaaatcctg gattaccgtc aaattgctaa gattcaatct acttatgtaa
     1921 ttggcttgca ggactggatt ttggctgatg gaaagattca tactcgctat atgcaggatt
     1981 tgacccagac cgggcgtttg tctagtgtgg atccaaactt gcaaaatatt cctgcccgat
     2041 tggaacaagg gcgcttgatt cggaaggctt ttgtgccaga gtgggaggat agtgtgctac
     2101 tcagctctga ctattcacag attgaattgc gcgttttggc gcatatttct aaggatgagc
     2161 acttgattaa ggccttccaa gagggggcag atatccatac ttcgacagcc atgcgggtct
     2221 ttggcattga gcgtcctgat aatgtgactg caaatgaccg tcgcaatgcc aaggcagtta
     2281 actttggagt ggtttatggg atttcagact ttggcttgtc taataatttg ggaattagtc
     2341 gtaaggaagc caaagcctac attgatacct actttgaacg ttttccagat attaaaaact
     2401 acatggatga agtggtgcgg gaggcgcgtg ataagggcta tgtagagacc ctctttaagc
     2461 gtcgccgtga gttgccagat atcaattcgc gcaacttcaa tattcgtggt tttgcggagc
     2521 gaactgctat caactcacct atccagggtt cggcagcaga tattctcaag attgccatga
     2581 ttcagctgga taaagccttg gttgcaggtg gttatcagac taagatgctg ttacaagtgc
     2641 acgatgaaat cgtccttgaa gtgcctaaat ctgaattggt agagatgaaa aaattggtga
     2701 aacaaaccat ggaagaagcc attcaactca gtgttcctct tatcgcagat gagaatgaag
     2761 gggcaacctg gtacgaggct aaataaaaag ggggctagtc ctcctttttt gtagtataat
     2821 tctgcaaacc ttttcaaaat gtgctatact gatgaaaaag gaggatttct atgagtcaag
     2881 aatttatcaa tccaagtgat ggcgtgattc gtcagtatct cgcaacgagt aaaacccttg
     2941 ctgtggtggg tttgtctgac cgtgaagaaa caaccagcaa tcgagtaaca aaggaaatgc
     3001 aggctcgggg ctataaaatc atcccagtca atcccaaggc ggcaggtggc gaaatcttgg
     3061 gtgaaaaggc ttatgctagc ctcgctgaaa ttccttttcc tgtagatatt gtcaatgttt
     3121 atcgtcgcag tgaatttctg cccgatgtgg cgcgtgattt tctcaaagct gatgccaaga
     3181 ttttttgggc acagctagga cttgaaagtt tagaagcgaa agagattttg cgtgatggtg
     3241 gatgtgatga tatcgtgatg aatcgctgta tcaagagaga acatactcgc ttgattgagg
     3301 aagcataaga aaaaggtagc tgatgggcta ccttttgtgt tatactcaat gaaaatcaaa
     3361 gagcacacta gcaaactagg aaactagccg cgggctgctc aaaacaccgt tttgaggttg
     3421 tagatagaac tgacgaagtc agctcaaaac accgttttga ggttgtagat agaactgacg
     3481 aagtcagctc aaaacaccgt tttgaggttg tagatagaac tgacgaagtc agtaacatat
     3541 acctacggca aggcgacgtt gacgtggttt gaagagattt tcgaagagtg ttagaaaatg
     3601 ccgataaggg tctgcatacc aagagtggtg aggatgatgg caatccagca gatggctcca
     3661 agaacaatgg attttccact ggatttgacc atagcgacca gattggtttt gagaccgata
     3721 gcactcatgg ccatgacaat aaggaattta gagagttgtt tgagaggggt aaagaaacta
     3781 ctggacacac ctagagaggt gagtagtgta gtgaggagag aggcaaggat aaagtaaagg
     3841 ataaaaagtg ggaagacttt tttcagttgt aggctttgct tgttttcttg ttggcgactt
     3901 tgccagtagg atagaaagag cgtgataggg ataatggcca aagtacgtgt gagtttaaca
     3961 atggttgcag actcgagggt attgctttgg taaagactgt cccaagcgct ggcggcagcc
     4021 gttacagagg aagtgtcgtt gaccgcagtc cctgcaaaga gggcgaagcc ttcattggat
     4081 agatgaagcc aggtgccgag ggttggaaag atgagcgcag ccaagacatt gaagaaaaag
     4141 ataacggaaa tggcttgggc tacttccttt tccttagcat ggataacagg cgctgtcgct
     4201 gcaatggcag aacccccaca gatagaagaa cctactccaa ccaaggtagc cagttttgta
     4261 tccagtgcaa agaaacgctg gaagaggtag gcaataatca gagctattga gatagtggac
     4321 aggatgacag ggagtgaaga ttggccaact gcgaagacct gcgagatatt gagaccaaaa
     4381 ccaagcaaga caacggcata ttggagcaac ttcttggaac taaaggtcaa accagcatcc
     4441 agttgtttat aggacgagag aaagggatgt aggagcatgc ctatgaaaat ggcaaaaacg
     4501 ggcgcgccaa tcacagggaa gaatcctcct aagtaccaag ataggatgga aatgagaagg
     4561 caggccaaga tacctgctcc attttttgat agaaatgaca taaaaacctc caaataaaat
     4621 ctattaccat tatagaccta taaacaggaa aagtaaaata gaaagtggaa agctattctc
     4681 taatgtattt ttgcggtcgg ggggcttttg tagtataata gagatacgtt ttgaaagtag
     4741 gaggtatcta tggacttaac taagcgcttt aataaacagt tagataaaat tcaagtttcg
     4801 ttgattcgtc agtttgacca ggttatttcg gagattcctg gggtcttgcg tttgaccttg
     4861 ggggaacctg attttacaac gccagaccat gtcaaggagg cggccaagcg agcgattgat
     4921 cagaaccaat cctactatac agggatgagt ggtctgctga ctctacgtca ggcagccagt
     4981 gactttgtta aggaaaagta ccaactggac tatgctcctg aaaatgaaat cttggttaca
     5041 attggggcga cagaggcttt atctgcgact ttgacggcta ttttggaaga gggagacaag
     5101 gtacttttgc cagctcctgc ttatccaggc tatgaaccga ttgttaactt agttggggca
     5161 gaaattgttg agattgatac gactgaaaat ggttttgtct tgactcctga gatgttggag
     5221 aaggccattt tggagcaggg tgataagctc aaggcggtta ttctcaacta tccagccaat
     5281 ccgacaggaa ttacctacag tcgagagcag ttagaggcct tggcagctgt tttacgcaag
     5341 tacgaaattt ttgttgtctg tgatgaggtt tactcagaat tgacctacac aggcgaagcc
     5401 catgtgtctc taggaacgat gttgagagac caggctatta ttatcaatgg tttgtctaaa
     5461 tcgcatgcca tgacaggttg gcgtttgggg ctgattttcg ctcctgcgac cttcacagcc
     5521 cagttaatca agagtcacca gtacttggtc actgccgcaa ataccatggc gcaacatgct
     5581 gcagtagaag ccttgacggc tggtaaaaac gatgcggagc ccatgaagaa ggaatatatc
     5641 cagcgtcggg actatatcat cgaaaaaatg actgctcttg gttttgagat tatcaaacca
     5701 gacggtgcct tctatatttt tgctaaaatt ccagcgggct acaatcaaga ctcctttgct
     5761 tttctgaagg attttgctca gaagaaggcc gttgccttta tccctggtgc agcctttgga
     5821 cgttacgggg aaggctacgt ccgcctatct tatgcagcca gcatggagac tatcaaagaa
     5881 gccatgaaac gacttgagga gtacatgaga gaagcatgat tcagtctatc acgagtcaag
     5941 gcttggtgct ttacaatcgc aattttcgtg aggatgacaa gctcgtcaaa atttttacag
     6001 agcaggttgg caaacgcatg ttttttgtca aacacgctgg tcagtctaag ctggcgcctg
     6061 ttattcagcc cttggtgctg gcacgatttc tcttgcgaat caatgatgac ggactcagtt
     6121 acatcgaaga ctatcatgag gtcatgactt ttcccaagat taatagtgac ctctttgtca
     6181 tggcctatgc gacctatgtg gcagctcttg cagatgctag tttgcaggac aatcagcagg
     6241 atgctccctt gtttgctttt ttgcaaaaga ctttggagtt gatggaagca ggcttggatt
     6301 atcaggtttt gaccaatatt tttgaaattc aaattttgac tcgatttgga atcagcctca
     6361 attttaatga gtgtgtcttc tgccatcggg ttggtcaggc ttttgacttt tctttcaaat
     6421 atggagcctg tctctgtcca gagcattatc atgaggatga tagacgttgc catctcaatc
     6481 ccaatatccc ctatctgctc aatcaatttc aagctattga ttttgagact ttggagacca
     6541 tttcgctcaa gcctggaatc aagcaagagc tacgccaatt tatggatcaa ctatatgaag
     6601 agtacgttgg gattcaccta aaatcaaaga aatttattga ttccctagca gactggggac
     6661 aattactaaa agaggaaaag aaatgaaaaa aatcgcagta gatgccatgg ggggcgatta
     6721 cgcacctcag gccatcgttg agggtgtcaa tcaagcccta tctgactttt cagatatcga
     6781 ggttcaactt tacggagatg aagctaaaat caagcaatat ctgacagcga cagagcgcgt
     6841 cagcattatc catacggatg agaagattga ttcggatgat gaacctacga gagctattcg
     6901 gaataagaaa aatgccagta tggtattggc agccaaggct gtcaaagatg gtgaagcaga
     6961 cgctgtcctt tcggctggga atacaggtgc cttgttggca gcaggattct tcatcgtggg
     7021 tcgtatcaag aatatcgacc gtcctggact catgtctacc ttgcctaccg ttgatggaaa
     7081 aggttttgac atgctagacc ttggtgccaa tgcagaaaat acagcccagc acctccatca
     7141 atatgcggtt ctaggttcct tctatgctaa aaatgtccgt ggcattgcgc aaccacgcgt
     7201 tggtttgctc aacaacggaa cagagagtag caagggcgac ccgcttcgta aggaaactta
     7261 tgaattactg gcggctgatg aaagtttgaa ctttatcgga aacgtggaag cgcgtgattt
     7321 gatgaatggc gttgcagatg ttgttgtggc agatggtttc acgggaaacg ctgtgctcaa
     7381 atccatcgaa gggacagcta tgggaatcat gggcttgctc aagacagcta ttacaggtgg
     7441 tggtcttcga gcgaaactag gtgccctcct tctcaaggac agcctcagtg gtttgaaaaa
     7501 acagctcaat tattcagatg ttggtggagc ggtcttgttt ggtgttaagg cacctgttgt
     7561 caagactcat ggctcaagcg atgccaaggc tgtttatagt acgattcgtc agatccgtac
     7621 catgctagaa acagacgtgg ttgcccagac tgcgcgtgaa ttttcaggag aataaaagag
     7681 atgacagaaa aagaaatttt tgaccgtatt gtgaccatta tccaagagcg acagggagag
     7741 gactttgtcg tgacagaatc cttgagtctg aaagacgatt tggatgcgga ttctgttgac
     7801 ttgatggagt ttatcttgac tctggaagat gaatttagta tcgaaatcag cgatgaagaa
     7861 attgaccaac tccaaaacgt aggagatgtg gttaaaatca ttcaaggaaa atagcaatcg
     7921 gagttccaag tcaacggaag tagatggttt ttagaaatga gaaatatcgg acaagctgga
     7981 aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga tatatcctca agcacaaact
     8041 ccacgtaaat ccagcaaact caagccgcta acagttgaag ataaagcctg taatcatgcg
     8101 ctatctaagg agagaagcaa ggttgagaat atctttgcca aagtaaaaac gtttaaaatg
     8161 ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct
     8221 ggtattatca atcatgaact aggattctag ttttgcagga agtctaatag taaaaaagtg
     8281 attagaaaac atctttttta aaaatagaga tgattttgaa acaaaaaagc taattcaaga
     8341 cgtttcgatg ccaattcaag atttggatga aaaaaattaa tagatactgt tatactaaac
     8401 ttgtcaagtt tgtaacaaga caaatattaa aaataaaaaa gaggtattcg ttatgaatac
     8461 aaaaacgatg tcacaatttg aaattatgga tactgagatg cttgcttgcg ttgaaggtgg
     8521 cggatgcaat tggggagatt ttgccaaagc aggtgttgga ggaggagcag cacgaggtct
     8581 tcagctagga attaaaacaa gaacatggca aggtgcagca actggtgctg tgggaggagc
     8641 tatacttgga ggtgtggcct atgcagcgac atgttggtg
//
LOCUS       DAVKLW010000078         8429 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKLW010000078 DAVKLW010000000
VERSION     DAVKLW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.078"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            9..1001
                     /locus_tag="VW324_001875"
     CDS             9..1001
                     /locus_tag="VW324_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9727442.1"
                     /translation="MNGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVI
                     EELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNAIVE
                     YFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVY
                     VSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVI
                     YGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFD
                     NIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1609..1686
                     /locus_tag="VW324_001876"
                     /pseudo
     CDS             1609..1686
                     /locus_tag="VW324_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2142..3503
                     /gene="trpE"
                     /locus_tag="VW324_001877"
     CDS             2142..3503
                     /gene="trpE"
                     /locus_tag="VW324_001877"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9727443.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3500..4066
                     /locus_tag="VW324_001878"
     CDS             3500..4066
                     /locus_tag="VW324_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9727444.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4077..5081
                     /gene="trpD"
                     /locus_tag="VW324_001879"
     CDS             4077..5081
                     /gene="trpD"
                     /locus_tag="VW324_001879"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9727445.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5078..5845
                     /gene="trpC"
                     /locus_tag="VW324_001880"
     CDS             5078..5845
                     /gene="trpC"
                     /locus_tag="VW324_001880"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9727446.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5832..6431
                     /locus_tag="VW324_001881"
     CDS             5832..6431
                     /locus_tag="VW324_001881"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9727447.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLPQSFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            6409..7632
                     /gene="trpB"
                     /locus_tag="VW324_001882"
     CDS             6409..7632
                     /gene="trpB"
                     /locus_tag="VW324_001882"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9727448.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7625..8401
                     /locus_tag="VW324_001883"
     CDS             7625..8401
                     /locus_tag="VW324_001883"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9727449.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIAGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
BASE COUNT         2492 a         1584 c         2035 g         2318 t
ORIGIN      
        1 tgaaaaatat aaatgggaaa aaggtaacta tttatgatat tgcacgttta tcaggttttt
       61 ctccaaaaac agtttcacga gttattaatg gtggagtaaa tgttaaagaa gaaacttatc
      121 aagcgattca aaaagttata gaggaattgt cgtatattcc aaatgcgtat gcaaaaaatt
      181 taactaaaaa agaagctata aatatattga tttctgtaaa aaaaatcgac tcttttcctt
      241 taatttggtt tcatacgctg ttagataaag tgttacggac gtgtaaagaa tttggtgtta
      301 atgctatagt tgagtacttt ggagaggagg atacaattag taattcaatt atatcaagca
      361 caggtagcct agtagatggt gttattgttt tttatgaaag tgtagatgat atcagaattc
      421 agtatttaaa gaagaaccat atgccttttc ttgttttcgg tgaatctcaa acgtctggag
      481 tagtctatgt atctaataat aattttcaag ctacttatga tatgatgaaa gctgtaactg
      541 aagaaaaatt taaaaatatg tggttgctta tgggaggaga atctcatgtt aataaggatc
      601 gtgaaagagg tgtgcgttct tttctgaatg ataaaaatta ctttatggat ttaaaagtca
      661 tttatggctt atctacaatt gattcagttt attcttatgc aatgcagcac ttgactaccc
      721 aaaattatcc ggacattatt tttgtttcag gagatgaaaa ggttcagggc ttgattcgtg
      781 cgtgctatga gaaaggaatt cttattccag atgatatttc tattattgga tttgataata
      841 ttcctatttc tcagtattat actccagctt tgtctacaat tgctcccaat tatgttaaat
      901 tggctaaaga aatgatagaa ggtgttttag caattattaa aggggaaagt gtcacatctg
      961 ttgaagtttc tcctaaattt gttaggagac agagtttcta gtattgttta attcttgaaa
     1021 atcctagtgg aaaagatgga gtgttctaaa ataagttctg tttagcctcc attcagtttc
     1081 tattgagttg gatccaattt ctaacaagtg ggacatgttc atgttcgatc taaggaagac
     1141 atggtcatat tcgaatgata ttttcaaaaa aatcgtaaaa aagtcttgac aaagaaaaaa
     1201 caaagtatta aaataagttc aacaaatcag aaaagtaact atttttgatc ttcagggagc
     1261 ctgtggtgat tgtgaatagg tggttggaag tagtgaaagt gggctgattt tgaaaatgaa
     1321 tttgaaacaa tgaaaattcg gtgcgcacac cttacagtgc agcttgttgt taaacaaggc
     1381 agagatgtaa agggatagtc cctttataat tgaggtggca ccgcgttacc aacgccctca
     1441 cacggaagta tattctgtgt gtgggctttt ttctatccgt cgtttggttt atcttttatt
     1501 agggccttaa gtcgctttga tgaacttgag ttctatctac agctccatgc ctactactaa
     1561 aagcaaacaa aaaggctgga ctaatacgaa aaaagctagt aatactcaat gaaaatcaaa
     1621 gagcaaacta ggaaactagc cgcaggctgc tcaaaacact gttttgaggt tgtggataga
     1681 actgacgaag tcagtaacca tacctacggc aaggcgacgt tgacgcggtt tgaagagatt
     1741 ttcgaagagt ataaaggtgt accgagatga acatctcttc atcaatgtct ctggtcgtgg
     1801 aggcaaggac ctagctgcga ttgcagatta ctagaagcta aaaaataata gatggaaaaa
     1861 ttacagttct ttctctcaca gagagcttgt ggttgctgaa aacaagcaga gaaagctgta
     1921 aagttgggtt catctgaaac gagagaagaa aacatcattc tcaagggagt gccctttatc
     1981 gcacagcctg ttacaggaga tatctgagac aggaatgaga gataggagaa atcctataat
     2041 tgaggtggca ccgcgaattt cgtcctcacg caagttattt tgcgtgggga tttttcatac
     2101 agtcaccacg gactagaagt agaactgaaa gggaaattac aatggaacga atcattcatg
     2161 gagatgtctt atcaccaatc ttggcttata tgcgcttaaa ggggcaacat aaggttattt
     2221 tagagagtat tccgagagac aaggaaacag ctcgtttttc tatcctagcc tataatccag
     2281 tttttgagat taagtttgaa aatggggttc tctatcaaaa tggtcaagtg attgatcggg
     2341 atcccttgga tttcctttat gaagtgattc ataagagtca gcaccactca gagctacctt
     2401 ttggtggtgg agccattggt tttgtgggtt acgatatgat ttcgctttat gaagaaattg
     2461 gtcaaatccc tgaggataca attgggacgc cagacatgca tttctttgtc tatgagagct
     2521 atatggtctt tgaccacaag aaggagaaga tacatgtcat agaggatgca ctttatagtg
     2581 agcgcagtca agaggccttg gaaaaatcct tgaaccaagt gcttgaggaa ttacgcattc
     2641 ctgctccaaa tgaatttgaa gatttggatc tatctccgtt agacttcaaa ccgcatatcg
     2701 ctccacataa gtttgaggga atggtagaaa cagctcgtga cttgattcgt aacggagata
     2761 tgttccaatg tgtactcagc cagcgttttt cagcagaagt tactggaaat ccatttgact
     2821 tctacagaaa tctccgcgtg actaatcctt ctaattacct ttatttctat gattttgggg
     2881 attatcaaat catcggagct agtccagaaa gtttagtttc tgtcaaaaat ggcatcgtga
     2941 caaccaatcc gattgcaggg acgcgaccaa gaggggctac ggatgaagaa gacaagacct
     3001 tggcgactga cctcctctct gatgagaagg aaacagcaga acatcgaatg ttggtagact
     3061 tggggcgtaa cgatattggc cgcatctctg aaacggccag tgtccaagtt actaagtata
     3121 tgaaggtgga gctcttccgc tacgtcatgc atttgaccag cgtggtcaag gggcgtttgc
     3181 ttccagaact cacggccatg gatgccttga aagctacact tcctgctgga accgtttctg
     3241 gagcaccaaa gattcgggcg atgagacgca tctatgaact ggaaacggaa aaacggggcg
     3301 tatatgcagg agcaatcggc tacttgtctg cgacgggtga tatggatttg gccattgcca
     3361 tccgaaccat gattctcaaa aatcaaagag cctatgtgca ggctggggca gggattgtct
     3421 acgactctat cgcccaaaac gaataccaag aaaccattaa caaagcaaaa tctatgacta
     3481 gaattggaga actaagacca tgattttatt gattgacaac tatgattctt ttacctataa
     3541 cttggcccaa tacattggga attttgcaga agttcaggtt ctgagaaatg atgattccaa
     3601 gctgtatgaa gaagctgaaa aagcagatgg tctggtcttt tctcctggtc ctggttggcc
     3661 agttgatgct ggaaagatgg aagacatgat tcgtgatttt gcaggcaaga agccgattct
     3721 tgggatttgt ttgggccacc aagccattgc agaagtcttt ggtggtaagc taggtttggc
     3781 tccaaaagtc atgcatggga aacagagcaa tatcaacttt gaagcgccat ctgttttgta
     3841 tcaaggtatt gaggaaggac gtgcggtcat gcgttatcac agtattttga ttgaggaaat
     3901 gccagaagac tttgaagtga cagctcgttc gactgatgac caagccatca tggggattca
     3961 acataaaaac ctaccgattt atggcttcca gtaccatcca gagagcattg gaacgccaga
     4021 cggcttgtct tctattcgga attttatcga gaaggttgta aagtgaggaa actaggatga
     4081 aagagattat tgaaaaacta gcaaaatttg aaaatttatc aggtgtggaa atgacggatg
     4141 tcattgagcg tatcgtaact gggcgtgtaa ctgaagcgca gattgcttct ctcctcttag
     4201 ctcttaagat gaagggggaa acacctgaag aacgcacagc cattgcccaa gtcatgagag
     4261 gacatgccca gcatattcca actgagattc atgatgccat ggacaactgt ggtacaggtg
     4321 gggacaagtc tttcagtttt aatatttcca caactgcagc ctttgtcttg gctggtggcg
     4381 gtattcacat ggccaagcat ggtaaccgct cgatttcttc taaatctggt tccgcagatg
     4441 tcctcgaagc cttgggaatc aatcttgacc tcaaaccagc tgaactaggt aaggtctttg
     4501 ataaaactgg aatcgtcttt ctcttcgcta aaaatatgca cccagctatg aaatacatca
     4561 tgccagctcg tttggaattg ggaattccaa cgattatgaa cttgactggt ccactgattc
     4621 atccaatggc tttagaaaca cagcttcttg gaattagtcg tccagaactc ctagaaagta
     4681 cagctcaggt tttgaaaaat atgggtcgca aacgtgccat cgtggttgct ggaccagaag
     4741 ggttggatga agctggcttg aacggaacaa ccaagattgc acttcttgaa aatggcgaaa
     4801 tcagcttgtc aagctttact ccagaggatt tgggaatgga aggctatgct atggaagata
     4861 ttcgtggtgg gaatgctcag gaaaatgcag aaattttgct tagcgttctg aaaaacgaag
     4921 caagtccatt cttggaaacg acagtcttga atgctggtct tggtttctat gctaatggta
     4981 agattgatag catcaaggaa ggagttgcct tggctcgtca agtgattgct agaggcaagg
     5041 cccttgaaaa actcagactg ttacaggagt accaaaaatg agtcaggaat ttttagcacg
     5101 aatcttagag cagaaggcgc gtgaggtgga gcagatgaag ctggagcaaa tccagcctct
     5161 gcgccagacc tatcgcttgg cagaattttt gaagaatcat caggaccgct tgcaggtaat
     5221 cgctgaggtc aagaaagcta gccctagttt gggagatatc aatctcgatg tggatattgt
     5281 gcaacaggcc cagacttatg aagaaaacgg agcagtgatg atttcggtgt tgacagatga
     5341 ggttttcttt aaagggcatt tggattatct acgggaaatt tccagtcagg tagagattcc
     5401 gacgctcaac aaagacttta tcatagatga aaagcaaatc atccgcgctc gcaatgcagg
     5461 tgcgacagtt atcttgctca ttgtggcagc cttgtccgaa gaacgcctca aggaactgta
     5521 tgactacgcg acagagcttg gtctggaagt cttagtggag actcacaatc tagctgaact
     5581 agaggtagcc cacagacttg gtgctgagat tatcggggtc aacaaccgca acttgactac
     5641 ctttgaggtc gacttgcaga ccagtgtaga tttagcccct tactttgagg aaggtcgcta
     5701 ttacatttct gaatctgcca ttttcacagg gcaggatgcg gaacgactag ccccatactt
     5761 taacggaatt ttggtaggga cagctcttat gcaggcagaa aatgtggccc agagaatcaa
     5821 ggagttgcag attgacaaag gttaaaattt gtggactatc gaccaaagaa gcggtggaaa
     5881 cagccgtttc agcaggagcc gactatatcg gttttgtctt tgcacctagt aaaagacagg
     5941 tgactttaga agaggcagct gagttggcaa agcttattcc tgcagatgtg aaaaaggttg
     6001 gagtatttgt ttcaccaagt cgggtagaac tgctggaagc gattgacaaa gttggcttgg
     6061 acttggttca agttcacggt caggtggcag atgatttatt tgagaatttg ccttgtgcta
     6121 gcattcaggc tgtgcaggta gatggagagg gtcatgtgcc caattctcag gcagactatc
     6181 tactctttga tgcccctgtg gctgggagtg gccagccctt tgattggggt caactggata
     6241 cgactggatt acctcagtct ttctttatcg caggtgggct taatgaagac aatgtagtaa
     6301 aagcaattca acactttact ccctatgcag tagatgtatc aagcggagtg gagacagatg
     6361 gacaaaaaga tcatgaaaaa attagaagat ttatagagag ggtaaagaat ggcatatcaa
     6421 gaaccaaata aagatggatt ttacggaaaa ttcggcggac gttttgtccc agaaacattg
     6481 atgacagcag ttttggagtt ggagaaggcc taccgtgaaa gtcaggcaga cccaagtttc
     6541 caagaggaat taaaccaact tttgcgccag tatgtgggac gtgaaactcc cctttactac
     6601 gcaaaaaact tgacccagca tatcggcgga gccaagattt atctcaaacg ggaagacctt
     6661 aaccatacag gagcccacaa gattaacaat gccttaggac aagtttggct tgccaaacgc
     6721 atgggtaaaa agaaaattat cgcagaaact ggtgctggtc agcacggtgt ggcaactgca
     6781 actgctgcgg ccctctttaa catggaatgt accatctaca tgggtgagga agatgtcaaa
     6841 cgccaagccc tcaatgtctt ccgtatggag cttttgggag ctaaggtcga ggccgtgaca
     6901 gatggttcgc gcgtgctcaa ggatgcggtc aatgcagctc ttcgttcatg ggtggctaat
     6961 atagatgata cccactatat ccttggttct gccttggggc ctcatccatt tccagaaatt
     7021 gttcgtgact tccaaagtgt cattggtcga gaggctaaac aacagtaccg tgacttgaca
     7081 ggacaaaatc tgccagatgc cctagtagcc tgtgttggtg gtgggtcgaa tgccatcggt
     7141 ctcttccatc cctttgtaga agatgagtca gtagccatgt atggagctga agcggctgga
     7201 cttggtgtgg atacggagca ccacgcagct accttgacca agggtcgtcc aggtgtcctt
     7261 cacggttccc tcatggatgt gctccaagat gcccatggtc aaattcttga agccttctct
     7321 atctcagcag gtttggacta tcctggtatc ggtccagagc attctcacta ccacgatatt
     7381 aaacgtgcca gctatgtccc tgtgacagac gaagaagcct tggaaggatt ccaactcttg
     7441 tctcgtgtgg aagggattat cccagccttg gaatctagcc atgctatcgc ctttgcggtg
     7501 aaattggcca aagaacttgg accagaaaag tctatgattg tctgcctatc aggtcgtggg
     7561 gacaaggatg tggttcaagt caaggaccgc ttggaagcag atgcagcaaa gaagggagaa
     7621 gctcatgcct aagacactaa cagaaaaatt aaatgctatt aaagcagctg gaaaaggaat
     7681 ttttgttccc tatatcatgg ctggtgacca cgagaaaggt ttggacggtc ttgctgaaac
     7741 aattcacttt ttagaagatt tgggtgtctc tgcaattgaa gtgggtattc ccttttcaga
     7801 ccctgttgca gatggacctg ttatcgaaga agctggcttg cgcagtttag cccacgggac
     7861 ctctacccag gctttagttg aaaccttgaa aaccattgaa acagagattc cactggtcat
     7921 catgacctac ttcaaccccc tctttcagta cggtgtggag aactttgtca aagatctggc
     7981 tgatacagct gtgaagggct tgattatccc agacctgcct catgaacatg ccaactttgt
     8041 agaaccattt ttggcaaata cagatatcgc cttgattcct ttagtaagct tgaccacagg
     8101 aattgagcgc cagaaagagt tgattgcagg ggcggaggga ttcgtctatg ccgttgccat
     8161 caatggggtg acagggaaat caggcaatta ccgtgcagat ttggacaagc acttggcgca
     8221 acttcatcaa gtggccgaca tcccagtctt gacaggtttt ggtgtatcta gtcaagccga
     8281 tctggaacgc ttcaatgcgg tgtcagatgg tgttatcgtc ggttcaaaaa tagtaaaagc
     8341 tctccaccaa ggagagccga ttcaggattt tatcagacaa gcagtagctt accaaaaata
     8401 atcaaacaag tagcaagtaa gaggaaagg
//
LOCUS       DAVKLW010000079         8394 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKLW010000079 DAVKLW010000000
VERSION     DAVKLW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.079"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..199
                     /locus_tag="VW324_001884"
     CDS             <1..199
                     /locus_tag="VW324_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU9727450.1"
                     /translation="IQKQNEIEELSINIEKKIMNYIFEDDGNVSEVIGALDIIHHLDK
                     IAHTTQAIYKWIMYRKYGNIN"
     gene            212..1686
                     /gene="galT"
                     /locus_tag="VW324_001885"
                     /pseudo
     CDS             212..1686
                     /gene="galT"
                     /locus_tag="VW324_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
     gene            1699..2712
                     /gene="galE"
                     /locus_tag="VW324_001886"
     CDS             1699..2712
                     /gene="galE"
                     /locus_tag="VW324_001886"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9727451.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKIDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YNL"
     gene            2893..3552
                     /locus_tag="VW324_001887"
     CDS             2893..3552
                     /locus_tag="VW324_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9727452.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3577..4644
                     /locus_tag="VW324_001888"
     CDS             3577..4644
                     /locus_tag="VW324_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9727453.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEAAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQTEIQVEYSKNFGSIPANKDALKDLPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            4747..5757
                     /locus_tag="VW324_001889"
     CDS             4747..5757
                     /locus_tag="VW324_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727454.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5769..7461
                     /locus_tag="VW324_001890"
                     /pseudo
     CDS             5769..7461
                     /locus_tag="VW324_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            7464..8174
                     /locus_tag="VW324_001891"
     CDS             7464..8174
                     /locus_tag="VW324_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9727455.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2851 a         1142 c         1636 g         2765 t
ORIGIN      
        1 tatacaaaaa cagaatgaga tagaagagct tagtataaat attgaaaaga aaataatgaa
       61 ttacatattt gaagatgatg ggaatgtttc tgaagtaatt ggtgctttag atattattca
      121 tcaccttgat aaaattgctc atactacgca agcaatttat aaatggataa tgtataggaa
      181 atatgggaac ataaattaga aagaaaaata tatgaaaaca attattgata attttgttga
      241 tagagtaatt gaatacggaa tgtataatga aattgacaaa atatatgtga aaaatagagt
      301 actagcgtta ataggtgagg agggaattga tcgcatttcc gatgaaaatg acctgaaaca
      361 aataaaagat tacttagtgg aaatcgcctt aaaaaacggt aaaattaaag atttaatcga
      421 ggaaaaggaa tgtttaggag cagagttaat gaatttcata gttcctttac ctagtcgatt
      481 aaatgatatt ttttggagtt catatgatat atcacctcaa gaagcagttg aagagtttta
      541 taagttaagt aaagatagtg attatattaa aacttctgct attgctaaga atattgagtt
      601 tcgagcatca actaaatacg gagagctaga gattacgata aatttgtcaa aacccgaaaa
      661 gatccaaaaa caattgctgc tgaaaaatta gtaaaagcaa ctaattatcc caaatgttta
      721 ctatgtatgg aaaatgaagg atatcagggg cgaataaatt atccagcacg ttcgaatcat
      781 agaattattc ggttgaaact tggtgacgaa gtatggggat tccagtattc tccctactca
      841 tactttaatg agcatgcgat atttttgaat agtcaacatg taccaatggc tattacctct
      901 aaaacatttg aacaattatt ggagattgtt gatattttgc caggatattt tgctggttca
      961 aattctgatc ttcctatctc aggaggctcc attcttagtc ataaccacta ccagggaggg
     1021 aaacatattt tcccaatgga aaaagcaaaa tttgagagtg aatttcgttt taaagacttt
     1081 gaggatgtta atgctggtat tgtaaaatgg ccaatgtcag taattaggtt acaaagtgaa
     1141 aataaaaatc gcttgttaga tttagctatt aaaattctaa ataagtggag agaatattca
     1201 gatttagaag tagacatcat tgctatgact gaagatgtcc cacaccatac ggtgactcct
     1261 attgctcgta aagtggacgg gaaatatgaa ttagatattg ttctaagaga taatcatact
     1321 actgaacaat acccagatgg tgttttccat cctcatcaag atgtacaaca tattaaaaag
     1381 gaaaatatcg ggttaatcga agtgatggga cttgcgatat taccacctcg tttgaaacca
     1441 gagttagaag aagttggaaa atatctttta ggagaggata acgcaattgc ggattatcat
     1501 ttagaatggg ctgatcaatt gaaagaaaaa tatcctaaca ttaaaaaaga agaggttaat
     1561 agcgtagttc aacacgaagt aggacaagca tttgcgagag tcctagaaga tgcaggtgtt
     1621 tataagaata cttcatctgg acatgaagcg tttatgagat ttgttaaatc ggttggaata
     1681 aattaggagg aaacaaaaat ggcaatattg gtaacaggcg gagctggtta tattggtagc
     1741 cataccgtag tagaattact aaatttagga aaggaagtta tcattgttga caacctttca
     1801 aactctagca tcttggtgct agatcgtatt gaagcaatta caggaatacg tcctgtgttt
     1861 tacgaattag atgtttgtga taaacaagca ttgagaaagg tatttgaaca agaatcgatt
     1921 gatgctgcaa ttcattttgc aggttataaa gctgtcggag aatccgtgca aaagcctgtg
     1981 atgtactaca aaaataatat tatgagtaca ttggcacttg ttgaagtgat gtcagaattt
     2041 aatgttaaaa agattgtctt ttcttcaagt gcgactgtat atggaattaa caatcagtca
     2101 cctctaattg agacgatgca aacaagtgct acaaatcctt atgggtatac gaaagtgatg
     2161 cttgagcaaa ttttaaaaga tgttcatgtg gcagattcag aatggagtat tgcgttgctt
     2221 cgttatttca atccaattgg tgctcatgag tctggtttga ttggggaaga tccctcagga
     2281 attcctaaca acttgatgcc ttatattgca caagtagcgg taggtaagtt gtcagagcta
     2341 agtgtgttcg gaaatgatta tgatacgctt gatggtactg gtgtgcgtga ttatatccat
     2401 gtagtagatt tagcaatagg acatataaaa gctttagaaa aagtatctga aaaaatagat
     2461 gtttatatct ataacctagg ctcgggagaa ggcactagtg tattacaact tgtaaataca
     2521 tttgaaagtg ttaataagat ccctattcct tataaaatag ttccaagacg ttcaggagac
     2581 gttgcaactt gttatgcaaa tgcagacaaa gcatataagg aattaaattg gaggacaaca
     2641 aaatcgattg aagacatgtg tagagataca tggaattggc aatcaaaaaa tcccaatggc
     2701 tataatttat gatactgtct atatgagtgc tgtattttgg aaaaattact taaactttta
     2761 atgaaatgca tatttaatga aagataaatt tttttaaaaa atgtttgaaa gcgcttgaca
     2821 aaggaaacgg tttcgttata taatataagc atagatgaca ccggtgtcaa atataaaaaa
     2881 gagggtcttt atatgaaaaa cctaacaaaa attaagttca aagaaaatgg agaatttaat
     2941 cattttccag gaaatactgt tgtagcaaat ctttatacta aacaagattt gatggaagtt
     3001 gttgatatta ttcaatcacg ttatagagaa ttaccattta tcgataagtt tactttaact
     3061 ccaaggaatt ctattcacat gactgtaatt gaattgttgt gccatgaaaa tcgtgaaacg
     3121 gagttttgga gcagtaatct tcctcttgat acacctttac aggaaataca tgattacttt
     3181 gctaaacaac ttgaaatttt tccattgttg gatgaagaaa ttcatatgcg tgtaactgaa
     3241 atgggaaaac aaaacatact agttgaacct gcagatgaag cttctgcaaa gagattagaa
     3301 gaaattcgta cttatgtctc agaaaaagca ggtgttcgtt tccctaatca tgatagatat
     3361 caattccata tttcaattgg gtatcttcgg attcctctaa ccgaagagga ggaagaagag
     3421 tttactaaag tcagagcaga attaactgaa attttattag agaagattcc aactattact
     3481 gtaaaccgta ttgactatac tgtatttgaa gatatgagac aatttgttcc atatcatgaa
     3541 aaatttaaat aaaataataa aaaaggagaa cttatcatga aaaaaatgaa agtttggtct
     3601 actgtacttg caacgggagt tgctcttact acacttgctg cttgctctgg aggttcaaat
     3661 tctacgactg cttcttcatc tgaagaaaaa gctgataaaa gtcaagaatt agtaatctat
     3721 tcgaactcag tctcaaatgg tcgtggtgat tggttaactg ctaaagcaaa agaagctggt
     3781 tttaatataa aaatggttga tattcctggc gctcaattag cagaccgtgt tattgccgag
     3841 aagaataatg cagttgcaga tatggtattt ggaattggtg ctgttgattc aaataaaatt
     3901 agagatcaaa aattactagt ccagtacaag cctaaatggt tagataaaat tgatcaatct
     3961 ttatcagata aagataatta ttataatcct gtgattgttc aaccattagt tttaattggg
     4021 gcgcctgatg taaaagaaat gcctaaagat tggactgaat taggtagtaa gtataaaggt
     4081 aaatattcaa tttctggtct tcaaggtggt acaggacggg caattctagc aagcatctta
     4141 gttcgatacc ttgatgataa aggtgaatta ggtgtttccg aaaaaggttg ggaagcagca
     4201 aaagaatatt tgaaaaatgc atacactctt caaaagggag aaagttcaat tgttaagatg
     4261 ttagacaaag aagatccaat acaatatgga atgatgtggg gttctggtgc attagttgga
     4321 caaaaagaac aaaatgttgt tttcaaagtt atgactcctg agattggtgt accatttgta
     4381 actgaacaaa ctatggtttt aagcactagt aaaaaacaag cgttagctaa agaatttatt
     4441 gattggtttg gtcaaacaga aattcaagta gaatatagta agaactttgg atctattcct
     4501 gcaaataaag atgccctcaa agatctacca gaagatacta agaaatttgt tgatcaagtg
     4561 aaaccacaaa atattgactg ggaagctgtt ggaaaacatt tggatgaatg ggtagaaaaa
     4621 gctgaattag aatacgtaca ataagaaata agagttaatt tttatgtcca agtctctcta
     4681 ttatttctaa atttatagaa gaaataaaga gaggcttttg gtttagtaat agagggagtt
     4741 agtgagatga ttaaatttga taatattcaa attaaatatg gtgattttgt tgcaattgat
     4801 aatctgaatt tagatataca tgaaggggaa ttctttacat ttcttgggcc ttcaggatgt
     4861 ggtaaatcaa ctactttgag agcattggta ggttttttag atccatcatc aggaagtatt
     4921 gaagttaatg gaacagatgt cactcatttg gaacctgaaa agcgtggaat tggtattgta
     4981 tttcaatctt atgcgctatt tccaactatg actgtttttg ataatattgc atttggttta
     5041 aaagttaaga aggtagctcc agatgttatt aaagctaaag tatcagcagt ggcagcaaaa
     5101 attaagatct ctgatcaaca gttacagcgt aatgtatcag aattatctgg gggtcaacaa
     5161 caaagggtag cattggctcg tgctctggtt cttgaaccta aaattctttg tctagatgaa
     5221 ccattgtcaa accttgacgc aaaattacgt gtagatttga gaaaagagtt gaaaagactt
     5281 caaaaagagt taggtattac tactttatat gttactcatg atcaagagga agccttgact
     5341 ttatctgata gaattgcagt ctttaacaat ggatacatcg aacaggtcgg tacaccagta
     5401 gagatttatc ataattctca aactgaattt gtatgtgatt tcattggaga tattaatgtt
     5461 ttgaccgatg aaacagtcca cgaagtatta ttgaaaaata caagcgtttt cttagaggat
     5521 aaaaaaggat acattcgatt agagaaagtt cgattcaatc gtgaaactga acaagatttt
     5581 attctaaaag ggacaattat tgatgttgag ttttctggag ttacaattca ctatacaata
     5641 aaagtttctg aaagtcagat tcttaatgta acaagtattg atagtcaggc tgctattaga
     5701 tctgtcggag aaagtgtgga attatttatc acaccatcag acgttctgca attttaagga
     5761 ggtgcagtat gcgtcataaa ttaaatttaa aagattggct tattcgttta gggttaatct
     5821 ggttcttagt aacatttatt atttatccaa actttgatct agtagtgaat gtatttgtaa
     5881 aaggaggaga attttccctt gatgctgtac atcgtgttct aaaatctcag agggcacttc
     5941 agagtattat gaacagtttt aagttagcat tttcactcat tattacagtt aatgtcgtag
     6001 gtattctttg tgttctattt acagagtact ttgatattaa aggtgctaaa attttaaaat
     6061 taggttatat gacctcttta atttatggag gagtggtttt agcgactgga tataaatttg
     6121 tctatggtcc ttatggattg attacaaaat ttttacaaaa tgttatccct tctttagact
     6181 ctaactggtt tattgggtat ggtgcagtct tattcattat gacattttca ggaactgcta
     6241 atcatacatt gtttttaaca aatacaattc gaagcgttga ctatcacact attgaggctg
     6301 ctcgaaatat gggagcaaaa ccatttactg ttttccgaaa agtagtgtta ccaaccttaa
     6361 ttccaactct atttgcactt actattatgg tttttcttag tggtttatct gcagtagcag
     6421 cacccatgat tgttggtggt aaagaatttc aaactataaa tccaatgatt attacatttg
     6481 cagggatggg gaattctcgt gatttagctg ccttacttgc aattatttta ggtattgcaa
     6541 ctacaatttt gcttactatc atgaataaga tagaaaaagg tggaaattat atttctatct
     6601 ctaagactaa agcgcctctt aaaaaacaaa aaattgcgtc taagccttgg aatatcattg
     6661 ctcacattgt agcatatgga ttgttcacag ttttcatgct tccactaatt tttatagtat
     6721 tatactcatt tacagatcca gttgcaattc aaacaggtaa cttaacatta tcaaacttta
     6781 ctttagaaaa ttatcgctta ttctttagta atagtgcggc attctctcca ttcttggtca
     6841 gctttattta ttctattatt gctgcgacaa cagcaacaat tctcgcagtt gtatttgctc
     6901 gtgttgtcag aaaacataaa tctcgttttg atttcttatt tgaatatggt gctctacttc
     6961 cttggttact accaagtaca cttttagcag taagtttatt atttactttt aatcagccac
     7021 aatttcttgt cttgaatcag attttggtag gtagtttggt aattctactt attgcatata
     7081 tagttgtaaa aatcccattt tcttatagaa tggtacgtgc tattttattt agtgttgatg
     7141 atgagatgga agatgcagca agaagtatgg gtgcttcacc tttttatact attgatgaag
     7201 gttatcattc catttatttt accggttgtt ctctctgtta ttgctttaaa ctttaactct
     7261 ttattaactg acttcgactt atctgtattc ctttaccatc ccctagctca accattaggt
     7321 attacgattc gatctgcagg tgatgaaaca gcaacatcta atgcacaagc tctggtattt
     7381 gtttatacaa ttgttctgat gattatttct ggaacggtat tatacttcac acaaagaccg
     7441 gggcgtaaag taaggaaata atcatgacag ccactagtct tgggttatca aatattgaaa
     7501 tagttgtcag gattgtttta tcagtagtca ttggtagtat aattggttta gagagaggga
     7561 gcaaatccca gcctgcaggc atccgaactt atagtattgt ttgtctagct gcatgtttga
     7621 ttatgatgac gaatgaatac gtatcttata aatttgggac aggagatcct acacgattag
     7681 gagctcaagt tatatcaggt gtgggttttc taggcgctgg aacgattctt attacagata
     7741 aaaagaaaat tacaggtctg acaactgcag caggcatttg ggcttcggca ggaattggat
     7801 tagctattgg agtaggtttt tatgagggag ctcttttagt agccatttct gtttggggtg
     7861 tgatatccat gttccaacca ctaaaaaaat atctgcaaaa tcgttctaaa atgattgaat
     7921 tgtatatagt ggttaaatct acagaagcct ataatcgtgt attggtttat tgtgcagaaa
     7981 atggtatcag aatgaccgat tcaagaacag cgtttggaga tgttaattct gacagaattg
     8041 aatactttga tgttccggac aaaaaaatag cgtcatttat tactctggaa ttatcaggta
     8101 gatttgaaca tcttcgactg atggaagaaa ttgctaatat tgtaggtgtt atttatgtcg
     8161 aagaaattag ctgaaattta gtaaacaaag ggacagatgt ttttgtcctt ttgcttttta
     8221 gatgttttaa tcatctaatt tttgagtaga tttttgctca gaggttttta tgaaagatta
     8281 taaaatcaat tttgatttag gtaaaataga atattttgat aataattgtt tgatacaagt
     8341 atacaaattt atttcttttt atgatatttg tgaaatggtg tttgcctttc attt
//
LOCUS       DAVKLW010000080         8333 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKLW010000080 DAVKLW010000000
VERSION     DAVKLW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.080"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..935
                     /locus_tag="VW324_001892"
     CDS             <1..935
                     /locus_tag="VW324_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9727456.1"
                     /translation="APQTIHTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSV
                     KKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLFYP
                     RKGKALKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDR
                     KSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAH
                     PYASWERGTNENHNRLIHRWLPKGTKKMTPKEVAFIEKWINNYHKKCLNYKSPREDFL
                     MANLNLKFSVRNKSRNRFKFCRSFKYPARRCSWC"
     gene            907..1551
                     /locus_tag="VW324_001893"
     CDS             907..1551
                     /locus_tag="VW324_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9727457.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1593..3581)
                     /locus_tag="VW324_001894"
     CDS             complement(1593..3581)
                     /locus_tag="VW324_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9727458.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVYFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3583..4341)
                     /locus_tag="VW324_001895"
     CDS             complement(3583..4341)
                     /locus_tag="VW324_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727459.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4769..4960
                     /locus_tag="VW324_001896"
     CDS             4769..4960
                     /locus_tag="VW324_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727460.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5314..5586)
                     /locus_tag="VW324_001897"
     CDS             complement(5314..5586)
                     /locus_tag="VW324_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9727461.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5645..6094)
                     /locus_tag="VW324_001898"
     CDS             complement(5645..6094)
                     /locus_tag="VW324_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9727462.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            complement(6081..8210)
                     /locus_tag="VW324_001899"
     CDS             complement(6081..8210)
                     /locus_tag="VW324_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9727463.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVNPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESETFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNINTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2522 a         1758 c         1624 g         2429 t
ORIGIN      
        1 aagctcctca aactatccac actgaaatca agcgtgggac agtccgacaa tgtcttggaa
       61 aagggcgctt caaagaggtt tattctgccg actatgctca acagtcttat gaaaacaatc
      121 gcaagcgctc ggtcaagaaa tcaagcttga ccaaggaact aaaggaaaag attctccact
      181 atcataacca aaaattttcg cctgaaatga tggtgatggc taaaggggtt aacgtgggaa
      241 tttcaaccat ttactattgg attcatcatg gaaaattggg gttaagcaaa caggatctgt
      301 tttaccctag aaaaggaaaa gcgcttaaga aacaggctag caccaacttt aaacctgctg
      361 gtcaatccat cgaacagcgg tctgaagcta tcaatcttcg cttggagaat gggcattatg
      421 agattgatac ggttctactt acgagagcga aaaactactg cttgcttgtc ttgacggatc
      481 gaaagagtag acatcagatc atccgattga ttccaaataa aagtgctgag gtggtcaatc
      541 aggctctaaa actcatctta aaacaacaca agattctttc catcacggca gataatggaa
      601 cggaattcaa tcgcttgttt gatgtatttt ctgaggaaca tatctactat gcgcacccct
      661 atgcctcttg ggaaagggga actaatgaga atcacaacag gctcattcat agatggttac
      721 ctaagggaac caagaaaatg actcccaaag aagtcgcatt catcgaaaag tggattaaca
      781 actatcataa aaaatgcttg aactacaagt cacccagaga agacttcttg atggctaact
      841 tgaacttgaa atttagcgtc agaaacaaat cacgaaatcg attcaaattt tgcaggtcgt
      901 ttaaatatcc tgcgcgcagg tgttcttggt gctaacgatg gaattatttc cattgctggt
      961 gtggttatcg gggttgccag tgccacgacc aatatctgga ttatcttttt atcaggattt
     1021 gcggctatct tagctggtgc cttttcaatg gctggtggag aatatgtatc cgtttcaact
     1081 ccaaaagata ccgaggaagc tgccgtttcg cgagaaaaac tcttgttaga ccaagatagg
     1141 gaactagcca aaaaatccct ctatgctgct tatatccaaa atggagaatg caaaacttct
     1201 gcccaactct tgaccaataa gatctttctt aaaaatccac tcaaggctct ggtagaggaa
     1261 aaatatggga ttgagtatga agaatttacc aatccttggc acgctgccat ttctagcttc
     1321 gttgcctttt tccttagaag tttgcctcca atgctgtcag tgaccatttt tccaagtgat
     1381 taccgcatcc ctgctaccgt ccttattgtc ggtgtggccc ttcttctcac tggttacaca
     1441 agtgctagac ttggaaaagc cccaaccaaa acagctatga ttcggaacct tgctattggt
     1501 ctcttgacca tgggagttac cttcctgctc gaacaacttt tcagcattta gaatacaaga
     1561 aatacctcga ttttgaagtc gaggtatctt ttttacattt gcacaatctt gcgataactt
     1621 cttgaagtaa tcatgaaaat cagcacatag gcgatgagga agatagcgca gatagacaag
     1681 gtcacaatca acatcatagt cgtatccagt acaccaatca cttttaaaat cagactaagc
     1741 atatggtagg caaaggctag atgtaggaag gcaaagagca aaggaaggaa gtatacagtt
     1801 aaaacctgtt tgttgatggt ttgcttgatt tgcttttgat ctaaaccgac tttctgcaag
     1861 ataataaagc gctcacggtc ttcatatcct tcagaaattt gtttgtagta gatgaccaga
     1921 acggttccga ccataaagat aatggataag aaaataccga taaagaagac accaccaaag
     1981 agggcactca tctgagcact agcatctgct agagtgctac catacacata gttaccttca
     2041 gtgtttaatt gagcattaaa cttttgtaag tatttttcat actcctcagc gaccttgagt
     2101 tgttctgctt cactggcatt tacattcata ccaccgtaaa actgattata gatagccgaa
     2161 tctgggaatt gatccaaaaa ggcttgtaaa tcaggtacaa caaggtaatt gtaatcagca
     2221 gtcaaaatat taaactgatt tggaacatgg ttgacaataa aatctttagt aaattcttct
     2281 tttacagaaa attgatgatc atttagagtt agagctttct gttctttaac tccctcattc
     2341 tttgcaaaga gtccgacctc atttcctgat agagacagtt tttgaccagt catattttca
     2401 taatcttttt ggtcaaatac catgaaaact gtttttggtt ggacacggtt ttgtcctttt
     2461 tcaaaaatag ttaacttggt tccttcttga ttcgcaacag caaagtaagt gtaacgaaat
     2521 acttcttttt ctttaatctt ataacctttg tcacttgcaa actggctcaa gagtttgtcc
     2581 aagtcttctt tttcaacatt ttgccctgaa accccaaaat catgaggatt tagaactttt
     2641 ttaaagcttt ctgaggaatt gaaaatgctt gtcgctgctg acatggttac caaaaccatt
     2701 gttgacaaga tagcgatggt ggctagtcca actgcatttt tcttcatacg gaaaatcaag
     2761 ttagaaacag atatgagatt atttggttgg taatagtatt tcttattttt ctttaagatt
     2821 tggaggaaaa cggtaatccc tgcattaaac aagagataag tcccaaagat aaccagtaaa
     2881 acagctatga agaaggttgt taaggctgta agaggatctt ttaccgtaag ggcaagataa
     2941 tagccaattc ctaaacttat ggaaccaaga atggtttgga gaggaaggaa gcgacctttt
     3001 ttctctccac tagctttctc acgagagagc tggagggcat tcatacgggc gattcgaagg
     3061 gcattcagga acatgaggcc taggaaaatc aaaccgaaga caacaagcac tgtaatgaca
     3121 actttcgtct ggaaggtagc aaccagctca accttcaatt tcattagttt gagcaggaaa
     3181 gcgaaaatta acttgtcaaa caaggctcca ataccgatac ccgctccaac agttagaatc
     3241 ccaaatacca ctaactcctt aaaggtcata ctgataagat gacgcttctc caagcccaac
     3301 atgccataaa ttcctagttc cttggaacgg ttcttcatga caaaactatt ggcatagaga
     3361 acgataatgg ctgacgcaag ggtgacgaca aacataccaa atccaagtgt agcctgaatg
     3421 gttgttcctc cacggatttc cgcaatctta ggattgaagg ttagagagta aaagagatag
     3481 gtgacagtga ctgccaagag aacagccagc gcaaaaggat agtagagttt gcggttttta
     3541 atcaagttcg ataccgctaa cttattggtt aatcgaaaca tactaattca cctcgcttgc
     3601 catgacagtc aaggtatcag agatttcttg gaacatctga cgctctgtct tctctccacg
     3661 gtagatttgg ttgtaaagaa tgccgtcttt gataaagaga acacgcttgg ccctgctagc
     3721 agctgctgtt gagtgggtta ccatgaggat ggtttgccca cgctcattga tttcattaaa
     3781 gacatcaagt aaggctgcag atgacttgga atcaagggct cctgttggct cgtccgcaag
     3841 gagaatttca ggttctgtga tgatggcgcg ggctactgct acacgctgtt tctgaccacc
     3901 agaaatctcg taagggtact tctcttgcaa ttggttgata cccagatttt cagctgtcat
     3961 caccaatttc ttcatcatct ccgttatagg tcttcttgac aagacaagcg gaagcaagat
     4021 attgtcctta acagacagag tatctagcaa gttaaagtct tggaagacaa atcctagctt
     4081 ttcacgccgg aaactagaag cctgtgaatt tttaatagtt gctgtatcag ttccatttaa
     4141 gtaaacctga ccacgacttg gtttgtccag catagctagg atattgagaa gagtggattt
     4201 accagaacca gactcaccca tgatggcaac atagtcaccc ttttctacgg taaagtgaat
     4261 atccttgagg gcttctactt ggttgccctg aaaacgtgtt ttataaattt tttgaacgtg
     4321 ttttacatct aaaagtgtca tgagaatctc ctttcttaat atcccataaa atgaaatgta
     4381 gcttgcatca tagtgcatcc cagctgaacg cgctcaattt ctttgtgact tggttttcta
     4441 gttttcttat gtacgatttg tttcatgatg ttactccttt gttctttatg agtctagttt
     4501 acatcaaaaa aagaccgctt gccataacct aaccttacaa aagagacttt aatcttacat
     4561 ttttgtaaga ttgggggaaa tataagcaaa attacttaga aaatgatttg atttcctatt
     4621 tttaaagttg taaaatataa gtgcgaaaag gcttactttg aaaaggagga agcgggaact
     4681 aacattttag taaggttacc tagatgacac accattaaat tgaacacttt caaatcgaac
     4741 tacctataag gaaagaaggt gcgctttaat gaaacataaa gaacatatac tcatcggatt
     4801 tctctatctg ctctctccat tcatcggtca gctcttagtt gaacacacac actttatcgg
     4861 tacagaattt accggcactg cttatgttat ttgctggtta tcagttgtta tcagcatcca
     4921 ccatttttcc aaaaatgtat tgtctcagca gcaaaaatag attaaaaaac tgagtttaaa
     4981 ttactgcttt tatagtaaaa tgaaataaga acaggacaaa tcgatcagga cagtcaaatc
     5041 gatttctaac aattttttag aagtagaggt gtactattct agttccaatc cactatagtt
     5101 tgtagatgaa gctggcgaaa tattattaat actattcaaa aatcaaagtg caaactatat
     5161 caacttcaca ttgccgacta aattacagcc tgcggctggc ttcctagttt gcactttgat
     5221 ttttattgag tataaaagta aaaaagcttt aaatcaagca gtttagacta ttgatttaaa
     5281 gctttttgcg tattccaaat cttttgtaaa acctcaccaa aaccagcctt cattatcgtc
     5341 aatggcttga gcttctttgc gtttacgtgg gcctgttccg tacatttctg cttcgatttc
     5401 ttccttggtt ggcatgatag aagctaggat gatatagata atcaccccaa gtccaaagtt
     5461 tgcgactgta aaaatggcaa acagaaaacg tacaagggta acatcaaagt tccacttgtc
     5521 tgacagacct gccaaaactc ctgaaatcat gcgatttcgt ctcattttat aaaatttact
     5581 gttcatgata tttcctcttt ctgctaggtt agacatagta tatcacgggt aggctcggct
     5641 ttcatcagtc ctcaggctga tttattagta gcaatttacc tcgacaaagt ccacagcgat
     5701 agcgtctggt atcaattctt cgcttacgct gatacctttg ctggcaggat tggcaacgat
     5761 agagcttgat tggcttggag ttactattgg gcaaggatgg tacaaaccgt aatccatcca
     5821 ctgctttcaa cagttcctta aaatcccgat ccttatgttg atagcctttc ccttgaaaat
     5881 agaggtgata atgacagagt tcatgtcgaa caattttcct aaaaacgtcc aaccccagtt
     5941 cctgataaac cttgggatta aaatccaaat gcccatcttt ggggaaaaat cgcccacctg
     6001 tcgaacgtag acgcctattc cactggacat gatggataaa aggtctgccg aagtcttcta
     6061 gtgaaacctg cttgacgtaa tcagtcagtt tcatttggag ctaggagaga cagattaact
     6121 ttttcacgtt cagtatcaat tttcttaacc caaacgctca ccaaatctcc aactgccacc
     6181 acttgactag ggtgtttgat aaacttgcga ctcatatggg aaatatggat gagaccgtcc
     6241 tcatgaattc cgatatcaac aaaagcaccg aaatcaacaa cgttacgcac cactccttct
     6301 agcttttgtc caaccactaa gtccttgata tctaggacat cttgacggag aactggagca
     6361 tcaaaggaat cacggaaatc tcgacctggt ttgagaagat ctgcaatgat atctttaaga
     6421 gtttctggac caaggtctaa ctcttgcgcc atttccttga ctgaaagcga cttgagtttg
     6481 ctttgggcct cttcattcag gtccttaatg tccaagcgtt tgaagagttc cttaacggca
     6541 gcgtaatttt ctgggtgaac tcctgtatta tcaaggatat tgctactttc ggggatacga
     6601 aggaaaccag ctgcctgttc aaaggcctta gcccccaaac gaggaacttt cttgatttgg
     6661 gagcgtgaag tgatttttcc ttcctcctca cggtatttga caatattttc agaaatggtt
     6721 ttattgagtc cagccacgtg agaaagaaga gctggactgg ccgtattgat attgacaccg
     6781 acttggttaa ccaccgtatc cacgacaaag tccagactct cagatagttt cttctgactg
     6841 acatcgtgtt ggtattgacc gacaccaatt gacttaggat cgattttgac caattcggca
     6901 aggggatctt gcaaacgacg ggcgatagaa atagcagagc gtttttcaac agtcaagtct
     6961 ggaaactcct gacgagcaag ttcactggca gagtagaccg aagcaccact ttcatttacg
     7021 atgacataac taacctcagg aaaatcttta agaacttccg ccacaaaggt ttcactttca
     7081 cgactagcag taccatttcc gatggcaata atctctacac cgtattgacc aatcaagtcc
     7141 gccaaatctt tcttggcttc ttcgatttga cgagctgatg ctgggttgac aggatagatg
     7201 acctgagttg tcagcatttt tcctgttgca tccacgacag ctaacttggc acctgtacga
     7261 aaggctgggt caaacccaag aaccacgcgc cctttcagtg gagcaaccaa gaggagattg
     7321 cgcaaattgt cagaaaaaag ttggatagct ccctcttcag ctttctcagt taattctgtc
     7381 cgaatacgac gctcaatagc aggcaagacc tttttcttaa cggattgctg aacaacttca
     7441 tcaatataag catttttcac cttgaaacga gtagcaaaga aggcaagaat acggtccgtc
     7501 gcatgttcaa aaccgatctt caagacacca agtttctccc cacgattgag agccaaggta
     7561 cgatagcctt gcatagttcc aactgtctct gaaaaatcat aataaatctg aaaaacctgc
     7621 ttttcatcaa gactttcatc cttggcttga gaagtgagtt tagagtgtct cagcacttcc
     7681 tgataagtca tagaacgcaa ggtcacatct tccgataagg cttcgaccaa aatatcaact
     7741 gcaccggtca aggcttcctt gccagtcgca aatccttcac agacgaactt ttcagcttct
     7801 ttctctaagt caactatatt ctgcaaaatc aagcgagcaa gaggaaagag tccagcttca
     7861 cgggcaatgg ttgccttggt acgacgcttt tccttataag gaagatagag ttcttcaacg
     7921 tctgctaatt tttcggcaac taagatagct tcttccaatt ccttggtcaa cttgccttgc
     7981 tcttgaatct tagctaagac agcttcctta cggtcattga gatttgtcag acttttatcc
     8041 aaatcaataa tagccttaat cgccacctca tccagactac cagtcatgtc cttacgataa
     8101 cgcgcgataa agggaatagt cgccccttca gctgtcaaac ttagaacggt atcaatttgc
     8161 tttaacgtca ctcccaaatc ctgagagatt ttttcatatt ttttatccat aaatctatta
     8221 taccacaagc tggaaggctt tctccaatcc ttttcatact caatgaaaat caaaaagcaa
     8281 actaggaagc tagccgtagg ctgctcaaaa tactgttttg aggttgtgga taa
//
LOCUS       DAVKLW010000081         8230 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKLW010000081 DAVKLW010000000
VERSION     DAVKLW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.081"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(47..529)
                     /locus_tag="VW324_001900"
     CDS             complement(47..529)
                     /locus_tag="VW324_001900"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9727464.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(624..2108)
                     /locus_tag="VW324_001901"
     CDS             complement(624..2108)
                     /locus_tag="VW324_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9727465.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            2261..3871
                     /locus_tag="VW324_001902"
     CDS             2261..3871
                     /locus_tag="VW324_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9727466.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKSVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(4015..5412)
                     /locus_tag="VW324_001903"
                     /pseudo
     CDS             complement(4015..5412)
                     /locus_tag="VW324_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            5588..7033
                     /locus_tag="VW324_001904"
     CDS             5588..7033
                     /locus_tag="VW324_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9727467.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLSDIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
     gene            7035..7766
                     /gene="cps4B"
                     /locus_tag="VW324_001905"
     CDS             7035..7766
                     /gene="cps4B"
                     /locus_tag="VW324_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9727468.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            7775..>8230
                     /locus_tag="VW324_001906"
     CDS             7775..>8230
                     /locus_tag="VW324_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEU9727469.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIA"
BASE COUNT         2343 a         1508 c         1862 g         2517 t
ORIGIN      
        1 acgtacggtg gtgtgagagg ggctagagat tatcccctac tcgattttaa atcacatgac
       61 gttcaaaggc atcatctgaa atcccttgtt ccaagatgag ttttgcccat tctttagcag
      121 agaagaggct gtggtccttg tagtttccgc aagattcgat ggttgtccct gggacatctt
      181 cccaagtagt agtttcagcg atttccttga gcgaatcctt gataacagct gcgattttag
      241 cactggtgtg acgtccccac ataatcatgt ggaagcctgt gcggcaacca aatggtgaac
      301 agtcaatcat gccgtcaatg cgggtacgga tgagtttggc taagaggtgc tcgatagtgt
      361 gaaggccggc agtagggata gagtcttcgt ttggttgcac caagcgaata tcataattgg
      421 agatgatgtc tccttttggt cctgtttctt ccccaatcaa gcgaacatag ggtgctttga
      481 caatggtgtg gtcaagttca aaactttcga caataacttc ttttgacatg gtaaatcctt
      541 tcagttttct tctctcatta tatcataaag gttgctcctg agacagagag aaaacctctc
      601 cgaggctgga gaggttgaaa tctttactta cgatataagc ggtcgtattg gtagtatggg
      661 tcaaaggtta cgttgatacc cagtttacga aggacattct tgtcttcatc agtcaagatg
      721 atggttgagt gggcttcgct tcctttgagg ttgccgagtt cttccatagc gcgggcagca
      781 tcaggatttt ctgtagctgt gatagcaagt gcaatcagga tttcatttga atgaaggcgt
      841 ggattgcggc taccgagatg atcgatttta agaccttgga ttggcttaac aacttccggc
      901 tcgattaatt ttacttcttt agcgatgtca gctgattttt tgatggcgtt gatcaaggca
      961 gcggctgtag ggccaaagag ttctgagttc ttaccagtga cgatttcccc atttggcaat
     1021 tcaaaggcta gggctggtcc accagtttct tctgcttttt ggcgcgcaac gacagcaacc
     1081 ttacggtctg caggtgtgat accgaggtcg ttcatgagca actcaatttt cttgacggca
     1141 gcttcgccaa ctttttcagc tttgaagtca agaactgttt gatagtaacg gcggatgatt
     1201 tcttgtttag aagcttcgac agcagcctcg tcatctgtaa tagcgaaacc aaccatgttg
     1261 acacccatat ctgtcggtga agcgtatggt gattttccga gaatacgttc caacatgcgc
     1321 ttgagcactg ggaagatttc gatatcacgg ttgtagttga cagtggtttc tccataggtt
     1381 tgaagatgga aggggtcaat catgttgaca tcatcaaggt cagctgtggc agcttcataa
     1441 gccaagttaa ctggatgatg aaggggaaga ttccaaacag ggaaggtttc aaatttagcg
     1501 tagccagact tgatgccatt gatttggtcg tggtacatat tggacataca cgttgccaat
     1561 tttccagaac cgggtccagg agcggttacg acaatcaagt tgcgactggt tttgatgtag
     1621 tcgtttttgc ccatgccttc tggggaaatg atgtgatcca tatccgtcgg atatcctttg
     1681 attggataat gaagataaga atcaattccg tttttctcaa gttggttgag gaaggcatct
     1741 gcagcgggtt ggccagcgta ttgtgtaatg acaacggaac caacaaaaat ccctaattca
     1801 ttgaatttgt caatcaaacg aagaacttct tggtcataag aaatgcccaa gtcgccacgt
     1861 gctttggaat gttcaatgtt gctagcatta atggcaatca caacctcaac ctgctctttc
     1921 aattcttgca agagcttgat tttgttgtca ggttcataac caggaaggac acgagcagcg
     1981 tggaaatctt ctaacatttt accgccaaac tctaagtaga gcttgccgtc aaattggtta
     2041 atgcgttcca aaatatggtc gcgttgtaaa ttcaaatatt gttcagaact aaaagcttgt
     2101 tttttcattt ttttacctct gacctctatt ataataaaaa attggaagtt aggaaactac
     2161 ggagctaaaa aagaaattaa aaagattaag caaacgcttg cacaaaattt taaaaagtgt
     2221 tatcatatac tatagattat gaaaataatg aggtaaacag atgcaagaaa aatggtggca
     2281 taatgccgta gtctatcaag tctatccaaa gagttttatg gatagtaatg gagatggagt
     2341 tggtgatttg ccaggtatta ccagtaagtt ggactatcta gctaagttag gaatcacagc
     2401 gatttggctt tctcccgttt atgacagccc tatggatgat aatggctacg atattgctga
     2461 ttatcaagcg attgcaacga tttttggaac catggaggat atggatcaac tgattgcgga
     2521 agctaagaag cgtgacattc gtatcatcat ggacttggtg gttaatcata cctcagatga
     2581 acatgcttgg tttgtcgaag cctgtgaaaa tactgatagc cctgagcgag actactatat
     2641 ctggcgcgat gaacccaatg acctagattc tatctttagt gggtctgctt gggaatacga
     2701 tgaaaagtca ggtcaatact atctccactt tttcagcaag aaacagccgg atctcaactg
     2761 ggaaaatgaa aaacttcgcc agaaaattta tgagatgatg aacttctgga ttgataaagg
     2821 tattggtggt ttccgtatgg atgttattga catgattggc aaaattcctg acgagaaggt
     2881 agtcaataat ggtcctatgc tccatcccta tctcaaggaa atgaatcagg cgacctttgg
     2941 agctaaggat ctcttgacag taggggagac ttggggagca acgccagaga ttgccaagct
     3001 ctactctgat ccaaaggggc aagaattgtc tatggtcttc cagtttgaac atatcggtct
     3061 tcagtatcag gaaggtcaac ctaaatggca ctatcaaaaa gagctgaata tcgctaagtt
     3121 aaaagaaatc ttcaacaaat ggcagacaga gttaggagtt gaggacggct ggaattccct
     3181 cttctggaac aaccatgacc tccctcgtat tgtctcaatc tggggaaatg accaagaata
     3241 ccgcgaaaaa tctgccaaag cctttgcaat cttacttcat ctcatgagag gaactcctta
     3301 tatctaccaa ggtgaggaga ttgggatgac caactatccg tttgaaacac tggatcaagt
     3361 agaagatatt gaatccctca actatgcgcg tgaggctctt gaaaaaagtg ttccgattga
     3421 agaaatcatg gacagtatcc gtgttattgg acgtgacaat gcccgtaccc ctatgcaatg
     3481 ggacgagagc aaaaacgctg gtttctcaac aggtcaacct tggttggcgg ttaatccaaa
     3541 ttacgagatg atcaatgtcc aagaagcgct ggcaaatcca gattctattt tctataccta
     3601 tcagaaactg gtccaaattc gcaaggagaa tagctggcta attcgagctg actttgaatt
     3661 gcttgatacg gctgataagg tctttgctta tatacgtaag gatggcgacc gtcgcttcct
     3721 agttgtggct aacttgtcca atgaagagca agacttgaca gtagaaggaa aagtcaaatc
     3781 tgtcttgatt gaaaacaccc tagctcaaga agtctttgaa aaacaaatct tagttccatg
     3841 ggatgctttc tgtgtggaaa tgactgacta gaatgagcaa acctcaagtt tttgaagctt
     3901 gaggtttttt actatagtgg attgaaacta gaatagtgca cctctgcttc taaaacattg
     3961 ttagaaatcg atttgactgt cctgatcgat ttgtcctatt cttatttcat tttgctatac
     4021 ttgtagttga ggagaattaa gctcctcact agtaaactct tcataatcct ttttattttt
     4081 atgaagatat tgtttgaaag atgtgagttt ccacggatgg gtttgtggag ggatatactt
     4141 gcgtctttct ttttttgttc tggttcttgt tcaaagtttt tcgaatagag ttcatgattt
     4201 agtagctcct ttgtgtgata gattttgtca gcgatattga ggtagatgtc tccgtcaaat
     4261 gcttttataa ctaatgcttt cgtctttctg atgaaataga cttcttttcc ttgttcggta
     4321 gggatatagt aacgattttg gaatcggata tggtgtccac tatcgacgac tctctccgcc
     4381 agtctagcca gaatgagatt cctttcagag gggttaggaa cctcctcgaa gacagagagt
     4441 tttgtcttgt ttccaaactg ttcattaaag gtttgaatgt aggaaagcag gaaggtattg
     4501 gcttcttcca aggtatgaat attgttttgt tccagttcga taggcaggcg agattgtagt
     4561 gtctgattga gtctttcgac tctcccttta gcttgaggga tagaggttgt ctcaaggaga
     4621 atccctagtt ggtgacaggc gtatccaaac tgtgtatggg tatcgtcctc cattttctta
     4681 gagttggagg cttgataggt aaagaccgtt ctcttatctg ttttaatttg aaggggaatg
     4741 ccgtgattgg ctaagatttg ttcgaggaca tgatagtaag cattcaagtt ctcttgttta
     4801 tcaaaataag cgcctaggat attgccagaa gcatcatcaa tggctaagtg taagttagat
     4861 gtttgggctc caaactaggc atgaggactg gcatccattt gaatgagttc accagcaaat
     4921 ttctttctgg gtctactagg atgtactttt ttagggtctt caaggaagtt ttcagccgtc
     4981 ggtaagattg gattgtctag gggttgattc aggttcagtt tagcttgttt tcttactctc
     5041 ttctttgtct ttctatggga cttaggcgac aggatatttt tcttatagag tatttttcta
     5101 acagtagtat cagagagctg aattccttct tcttcagcta gcaattcaca gaaatgaagg
     5161 acatttggtt tatatgtttc ataggagagg tattttttta ggagacgttc tttgatttca
     5221 tcagggattg catgttttgg ttttcgattt ctgtttccgt gtctgaaggc ttctttgtcc
     5281 tttcagttga taggctagta gcagacgatt gatttgtctt tcagaaagat tgagctcgac
     5341 acaggctcgt ttctttgttt tctttccttg ggctatagct tttatcacaa gatatttttt
     5401 cgtttcattc atattcagtt ggatcctttt catatgacta ttctaccaaa tgggacattt
     5461 tcacgttcga tttactaaag acattatcac attcgaatta cacaagatgc agatagtaaa
     5521 aaaaaatgta gacattaccg taaaaaagtg atataatcgt atgatgttca aggtataggt
     5581 gttaatcatg agtagacgtt ttaaaaaatc acgttcacag aaagtgaagc gaagtgttaa
     5641 tatcgttttg ctgactattt atttattgtt agtttgtttt ttattgttct taatctttaa
     5701 gtacaatatc cttgctttta gatatcttaa cctagtggta actgcgttag tcctactagt
     5761 tgccttggta gggctactct tgattatcta taaaaaagct gaaaagttta ctatttttct
     5821 gttggtgttc tctatccttg tcagctctgt gtcgctcttt gcagtacagc agtttgtggg
     5881 actgaccaat cgtttaaatg cgacttctaa ttactcagaa tattcgatca gtgttgctgt
     5941 tttagcagat agtgagattg agaatgttac gcaactgatg agtgtgacag caccgactgg
     6001 gactgataat gaaaacattc aaaaactact atctgatatc aaatcaagtc agaataccga
     6061 tttgacggtc aatcagagtt cgtcttactt ggcagcttat aagagtttga ttgcaggaga
     6121 gactaaggcc attgtcttaa atagtgtctt tgaaaatatc atcgaatcgg agcatccaga
     6181 ttacgcatcg aagataaaaa agatttatac caagggattc actaaaaaag tagaagctcc
     6241 taagacgtct aaggatcagt ctttcaatat ctatgttagt ggaattgata cttatggtcc
     6301 gattagttcg gtgtcgcgtt cagatgtcaa tatcctgatg actgtcaatc gagataccaa
     6361 gaaaattctc ttgaccacaa cgccacgtga tgcttatgta ccaatcgcag atggtgggaa
     6421 taatcaaaaa gataaattga cccatgcggg catctatgga gttgattcgt ccatccacac
     6481 tttagaaaat ctctatggag tggatatcaa ttactatgtg cgattgaact tcacttcttt
     6541 cttgaaattg atcgatttgt tgggtggggt agatgtttat aatgaccaag attttacatc
     6601 tctacacggg aagttccatt tcccagttgg aaatgtccac ttagattctg agcaggctct
     6661 gggatttgtt cgtgagcgct attccctagc ggatggtgat catgaccgcg ggcgcaatca
     6721 acaaaaggtg attgcggcca tccttcaaaa attaacttcg agcgaagcgc tgaaaaatta
     6781 tagtaggatc attgatagct tgcaagattc tatccaaaca aacatgccac ttgagaccat
     6841 gataaacttg gtcaatgctc agttagaaag tggtggaacg tacaaagtga attcgcaaga
     6901 cttgaagggt atgggacgga cggatcttcc ttcctatgcg atgccagata gtaacctcta
     6961 tatgatggaa attaacgaca gtagccttgc atctgtcaaa actgctattc aggatgtgtt
     7021 ggagggcaga tgaaatgatt gatattcatt cgcacatcgt ctttgatgta gatgatggtc
     7081 ccaagtcaag agaggaaagc aaggctctct tggcagaatc ctacaggcag ggggtgcgaa
     7141 ccattgtctc tacctctcac cgtcgcaagg gcatgtttga aactccggaa gagaagatag
     7201 cagaaaactt tcttcaggtt cgggaaatag ctaaggaagt ggcgagtgac ttggtcattg
     7261 cttacggggc tgaaatttac tacacatcag atgttttgga taagctggaa aaaaatcgga
     7321 ttccgaccct caataatagt cgttatgcct tgatagagtt tagtatgaac accccttatc
     7381 gcgatattca tagtgccttg agtaaaatat tgatgttggg aattactcca gtcattgccc
     7441 acattgagcg ctatgatgca cttgaaaata atgaaaaacg cgttcgagag ctgatcaata
     7501 tgggctgtta cacgcaagta aatagttcac atgtcctcaa atccaaactt tttggagaac
     7561 gttataaatt catgaaaaaa agagctcagt attttttgga gcgtgacttg gttcatatca
     7621 ttgcaagtga tatgcataat gtggacagca gacctcccca tatggcagaa gcatatgacc
     7681 ttgtttccca aaaatacgga gaagcgaagg ctcaggaact ttttatagat aatcctcgaa
     7741 aaattgtaat ggatcaacta atttaggaga aatgatgaaa gaacaaaaca cgatagaaat
     7801 cgatgtattt caattactta aaaccttgtg gaaacacaag ctaataattt tattagtggc
     7861 acttgtgaca ggggcgggag cttttgcata tagcactttt attgttaagc cagaatatac
     7921 aagcaccacg cgtatttacg tagtcaaccg taatcaagga gataagccgg gactgacgaa
     7981 tcaggacttg caggcaggaa cttatctggt aaaagactac cgcgaaatta tcctttcgca
     8041 agatgtattg gaaaaggtag cgacaaattt gaaattggat attccagcaa aaacgttaac
     8101 tagcaaagtg caagtgactg taccagccga cactcgtatc gtctcaatct ctgtcaagga
     8161 taaacaacca gaggaagcca gtcgcattgc taattctcta cgagaagttg ctgcagaaaa
     8221 gatcatcgct
//
LOCUS       DAVKLW010000082         8160 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKLW010000082 DAVKLW010000000
VERSION     DAVKLW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.082"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            124..288
                     /locus_tag="VW324_001907"
     CDS             124..288
                     /locus_tag="VW324_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9727470.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAEMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            372..938
                     /gene="amaP"
                     /locus_tag="VW324_001908"
     CDS             372..938
                     /gene="amaP"
                     /locus_tag="VW324_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9727471.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            950..1120
                     /locus_tag="VW324_001909"
     CDS             950..1120
                     /locus_tag="VW324_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9727472.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1159..1767
                     /locus_tag="VW324_001910"
     CDS             1159..1767
                     /locus_tag="VW324_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9727473.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1798..2001
                     /locus_tag="VW324_001911"
     CDS             1798..2001
                     /locus_tag="VW324_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9727474.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2112..2384)
                     /locus_tag="VW324_001912"
                     /pseudo
     CDS             complement(2112..2384)
                     /locus_tag="VW324_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2693..2980
                     /locus_tag="VW324_001913"
     CDS             2693..2980
                     /locus_tag="VW324_001913"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9727475.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3258..4028)
                     /locus_tag="VW324_001914"
     CDS             complement(3258..4028)
                     /locus_tag="VW324_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9727476.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGYLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(4043..4669)
                     /locus_tag="VW324_001915"
                     /pseudo
     CDS             complement(4043..4669)
                     /locus_tag="VW324_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(4575..5177)
                     /locus_tag="VW324_001916"
     CDS             complement(4575..5177)
                     /locus_tag="VW324_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9727477.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSTGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGRKGGISSSCWGSVVVTSSERLLETSLR"
     gene            complement(5187..5504)
                     /locus_tag="VW324_001917"
     CDS             complement(5187..5504)
                     /locus_tag="VW324_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9727478.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5521..5838)
                     /locus_tag="VW324_001918"
     CDS             complement(5521..5838)
                     /locus_tag="VW324_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9727479.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(5853..6749)
                     /locus_tag="VW324_001919"
     CDS             complement(5853..6749)
                     /locus_tag="VW324_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9727480.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(6752..8014)
                     /locus_tag="VW324_001920"
     CDS             complement(6752..8014)
                     /locus_tag="VW324_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9727481.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         2804 a         1602 c         1282 g         2472 t
ORIGIN      
        1 ttaatcaaag aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg
       61 atcaattatt gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaagtagt
      121 tctatgggaa tcatcgcaaa tgtattcgct ggtttagtcg gggcatatgc aggacaatct
      181 cttttaggta gttggggtcc atcaatcgct gaaatggctt tgctcccatc tattgtaggt
      241 gcagcgattg tgattactgt agtgtcattc tttacaggta gaaagtaaac ttttcgccag
      301 taaagttagc aaactatttt taaatcaatg acgggaaaaa tagtttaaat gttaaatcga
      361 aaggattgta tatgtcaaaa gcaaagaaaa tatgtttcat tattttctgt attttaatct
      421 tgacaatttt ccttcctgtt ttgatagatt atcatcaagt tagtgatcta ggtattcatc
      481 tacttagctg gagacagaac tccgtagttg aattctatct tgctagatat gtcttttggg
      541 ggacagtggt tctatcaact ttagttttat tatccatttt agttgtgatg ttttatccta
      601 aacgttactt ggaaatccaa cttgaaacta aaaacgatac attaaaatta aagaattcgg
      661 caatcgaagg ttttgttaga agtttggtga gtgatcatag attgatcaag aacccaactg
      721 ttcatgtaaa tttacgaaaa aataaatgtt tcgttcatgt agaaggtaaa attcttcctt
      781 cagacaacat cgctgacaga tgccaaataa ttcaaaatga aataactaat ggattgaagc
      841 agttttttgg tattgagcgt caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa
      901 aacctcaaaa caaaaagact gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct
      961 taaacaatat cgatatccaa ttatcgctgg tctcataggc gtatttctgg cttgtttgat
     1021 tgtctccttt ggcttcttca aaacaatatt tgtattgatt ttaggagcac tgggagttgc
     1081 agctggatta tatatcgaaa aaaactatat agataaataa aaaaataaaa attactaatt
     1141 taattaaagg agtttcatat gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa
     1201 gatgctactg ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc
     1261 caaaaaatca ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt
     1321 ttcttctcaa atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac
     1381 gtagaagttg gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa
     1441 aatgttccag ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg
     1501 actgacttgg aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag
     1561 catgaagcag actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga
     1621 gaattcactt cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact
     1681 gttcaagaaa aagttagcga aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta
     1741 tctcacgaaa acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg
     1801 tcagtagaag aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc
     1861 atcggtgatg aaaaaatgga aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa
     1921 gaagttgccg aagacgctaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg
     1981 ttgagtggcg acgataaata aggttaaaag ttactttatc ttttttggta acattagtca
     2041 aaagagtctg agtcaagatg attctcagaa aacaaaaagc tagagattcc caattgcgga
     2101 actctagctt tttaattttg cctctttctc ttattatatt tcagcaggtt gttggccatg
     2161 agtatgaatc ccatgtcaat tctcacttga cgcttacctc tcagatgaca tctcttataa
     2221 cccaaacaaa cctttatctg cccaaagaca gatttcatat caatcttacg tttagcgaaa
     2281 atttgtctac ccttggaaga taaaagtgcc tgatattctt tagtttttaa acactggtaa
     2341 cgttcattca tatacagtcc cttttgaggg gctgattcag gttcataatc gcagtcaaca
     2401 ttgatttcaa ggctgtttgc tttctatctc ccctttttca taatgtatag taaaatgaaa
     2461 taataacagg acgaatcgat cgggacagtc aaatcgattt ctaacaatgt tttagaagta
     2521 gaggtgtact attctagttt caatctacta tattttcgta caggtgcttc aatagtttct
     2581 atagtaaaat gaaccaaaaa tagtacacgt tgcggtataa tgttcttgtg gcatattcat
     2641 tagattttcg taaaaagttc tctcctattt gtgagcgaac aggtatagtc aattgattgt
     2701 cagtttgaag accaagagtc gtaaggcaaa ggatatggca gaatccatcc aagggtggct
     2761 ggcacaattt ctagtgaacc tctttaaatc tattacgttt gactgtggaa aagagttttc
     2821 aaaatggaaa gatatttcca atcatcacga tagtgagagc ttttttgcga atctaggatg
     2881 tccaagacag cggtgtctca acgaacattc aaatagactt ttaaggtgtc atgacttgcc
     2941 aaagcagaca gatttcaacg aagtcagtca agaattttag gctgggtaaa aactcaattt
     3001 caggagaaaa tgaaataaat attcccacaa taaaacgcat agtatcaaag tttttccaac
     3061 acctgatatt atacattttt taattttaaa gactttttcc cagcctcaag aaatcacatc
     3121 ccacctaaat cctttggata ccaaatacct catcaggtat tttctgagtt ggtaagactg
     3181 tctagcttaa tttgacaaat ggagggcgtt aaatccagtt tttacaggaa gtctatttaa
     3241 atattttcac actgacctta caaaatatga aataaattat ataaagtcat tcccatgaca
     3301 aagaataaca tccctttaaa acagtatctt acccaatttt caggtaagac ttttgataca
     3361 accgtcccca atacccctcc gcatatagaa gcaagaaaaa taactggaac aggtgctaaa
     3421 tgatactgat ttaaacctgt aaccacaaca attgtagcca aatgagacag ctgggaaaag
     3481 aaaataattg caagagaata catcgttgct tcttttatcg aaatagaaaa aaacaccatc
     3541 aataaagata cattgagcgg accaccacca atccctaaaa aactagaaat caagcccaat
     3601 agaagaccac aaatcatata gaatcctatc ttatcaaatt gataagtttt tttaaaatct
     3661 gttaaaacaa atgatacaag taacatcaca aatagtaaaa tcatttgaac aatagatact
     3721 agccgtactg ataattgaga taacaaaact tgaaaaataa gataacctag aaatcctcct
     3781 aaaacagaag aaaaggatac cgtaaaaatc aatctccatt ttatctcttt actttgtgaa
     3841 aatcttttga ttgttgaaat aatcgccatt ataaaaacag agaaactaga ataaaaagca
     3901 atatcactga ccgaatggta tccaaaacta tccataagtg gcttaattat aattccacca
     3961 cccatgcctg atatagaacc caatccacta gctattataa tgattagaaa ataaataatc
     4021 tcaaccactt tttaagtcca tcctattctt tcaaacatct aaaacctaga tggctactag
     4081 tggaagtaga aatacatcca tttctagcag ctactcggta tcgattacaa taagaacaat
     4141 gacataaaaa ggaacctcct ctgatagcat actcctcatc ttgtatccca tattttggta
     4201 actcatagtc atcttcatta aaactagcta ataaggtata tctcggattg cgacaccatt
     4261 cccaaacatt cccaatcatt tgatatagac caaaatcatt aggtaaaaat tcatagacag
     4321 gagccgttcc tataaatcca tctaaggcag tattttcata aggaaatctt ccttgccaag
     4381 tattggcatg ataatatcca ccttctaaaa gagtatctcc ccaaagatat tcagattgtc
     4441 ttcctcctct agcagcatat tcccactgtg cttcagtcgg taaagacatt ccgctccaat
     4501 tacagaaagc caatgcatct tctaaagcaa catgaactac tggatggtct tctaaaccca
     4561 ctagattact gttctcaccg tagggatgtt tccagcaagc gttcggaact tgtaaccacc
     4621 acggagcccc agcaggatga ggatatccct ccctttctgc ctctggcatt aatatggttt
     4681 cctggaatca cattcccaat agttaccatc agatttttac catcaaatgc tagattatca
     4741 attccttgta agatctgatc taatttcaca tctcctacat ttgccaaagg gaaactcttt
     4801 gtaactgtac ttccaatcga aatgacaaac gaagcaagtg ataccaaacc agtagaaact
     4861 gtatcaacct tgtaaatctt agcgatattc actcccaagc aatagatgaa caacaaggaa
     4921 acaattggta tacttccctt gaataccaaa ttattgatgt caacaagcca ctgaaaggtt
     4981 ttcgtaatac tttctaggtg aaattgttgt ggtaaatcca ctagaaaagc atttaataac
     5041 aaagcaatgg aacctgtcat aataacaggc atagtcccca caaatgaatc acgtaaagca
     5101 actaaaaact tctgattccc aactttcatt gcaattggag aaagtttttc ttgcatttta
     5161 tccaacattg tattcatacc aaactcctaa tttttttcaa atcgttcgta tacttctacc
     5221 acttctttcg ccaagtcaac aaaagtcaga ctcgtcatta aatgatcttg cgcgtgtacc
     5281 atcaagaggc taattgatgt tttttccccc tgtgcctctc ttgtcaacat ttctgtttga
     5341 gccttatgcg cctgtaataa ggcagcattc gcatcagcta atcttcgatt ggctttagag
     5401 aaatcccctt tttttgctgc ctgaatagcc tccatagcat tacttttggc ttccccacca
     5461 tacataatta gccccattac agattctaaa ttactttcat ccataacatt ctcctcaatc
     5521 ttagtctaat aaactttcag ctaaatctaa aactttagaa ccattcatca ttccgtaatc
     5581 tgtcatcgga ataaccgcca caggaatctg tctatctttt agtttttctt gaaagtcccc
     5641 tagtaaatag cgaacttgag gacctaaaag caacacattt acttcttttg ttgctacaat
     5701 ttcttctgct tcaggagctg gaactgcaaa aatttctgca tccaacccct tatcttctgc
     5761 tgccttttgc atctttgtca ctagcagact tgtactcata cctgctgcac atgctaacat
     5821 aattgttact ttagccatag cttactcctt tatcacctat ttactgacct agccaagcga
     5881 gatactgtat gtctcactcc tctaattgcc ttgctcaatg cataaatatt atcgactgtt
     5941 gccagtcccc catatcctgc gtcacctata tgttgaatat caactccaca aattttattt
     6001 ctaagtgcaa tttccttgat agtatctgta tcagatgttt cttggctagt accaatagca
     6061 ctcaatacaa gaccaccctt actatgaacg agatcaacga cttcacgcaa ctcttggtca
     6121 tgaaaggctg gaacggttcc aactgctgga acaagtatca catccgcacc tgcttccaac
     6181 aattgctctg cgacagaaag ctctgccaca ggctcattca ctcctgcccc gtgcatttta
     6241 ccagctataa tcaaacctga gaaattctct ttagcagatt gaactgcttt gataatctct
     6301 tgattgctaa ctcctgttcc aggatttcca gtcaaacaga caaagtcaaa acctaactcc
     6361 tctattcgat tcaatgtttc aacactagca acacgacctg caacaatttc ctgtgtttcc
     6421 tctagcatct ttgcagatgg gtcaatcggt tccaaattta caccaattgg acaagcaaca
     6481 agctggtgta agcgatgaat aacatcatca cctgaactat ccaaagcata aattttaggc
     6541 tcaaaaacat caacacaatt caataaaatc atatcagctc caaaagctct agcaatttca
     6601 gaattagtta tatccccaac aaatgtctca cgagtaacta cattttcaga tagaacaact
     6661 ctaccttcac ttgctaaaat actttgtttt aattcttcag cattcatctg taatatctca
     6721 gatggatttg cactaattaa acgtttcatt ctcaatcctc caataactta aaccgttttt
     6781 caatagctat tacaaatggt aaataaagta agacatccaa aacaatcaat acaatttgta
     6841 ataatgaacc agaaatactt cctgtagcaa gtaaaccact tataacaggt ggcatagtcc
     6901 aacctggatc tgtataagtt agcggaacca aacctgatgc cattgctccc cacgctacta
     6961 gtaagttaaa cattggagct aagataaatg gaactagtaa caaaatgttc aaaacaatag
     7021 gaacgccaaa cattgctggt tcatttatat taaataatcc aggtataaca gttataggtg
     7081 ccaaagtttt tagttgttta ctagctttct tcttatgtgc taaatatcct aacgcaagaa
     7141 ctaaacctat ggttgcacca ccgccaccta tgaatacaaa attatccata aatacgctag
     7201 taaaaatatt tggaagagtt tctccagttt gataagctac tttatttgca tctagattag
     7261 ctaaccataa cggtttcata actgcattaa caacatttcc tccgtgtaat cctacaaacc
     7321 aaaaactact attaaggaaa attaagataa gaagaccaat aacattattt cctaaaagtc
     7381 caagtggacc tcccaaagct acttgagcaa tttcatgaag attaggtaaa cttaatttat
     7441 ctaaagttgc ataaacaatc aaccaaccaa caatagtcac tgcaccagga ataatggcac
     7501 taaagctttt agatactgct ggtggaacac catccggcat ttttatctgt atattatgat
     7561 ttataaacca ttggtagata tagccattaa tcaatcctaa aatcatagca acaaataaac
     7621 cttttgatgc catgaaagcg gtaggcattc ctgctccagc ctctcctcta atgaaaggcg
     7681 taactgtaat aaaagatgat aatgacacaa taccagcagg aataccatca actttgtatt
     7741 gtctagccat aaagtaagcg ataccaaaac tagctacaag accaagtaat ccaaaactac
     7801 tatcaacacc cttccataaa tagtcagcaa caccaatatc acccagatat ttttcccatc
     7861 ctggaatagg aaaacttgca ataaccatta gtaatgaacc tatcaacaat aaaggtatag
     7921 ttagagtaat accgtctcga atagaaacca acgctttatt attacccaat ctagccgcta
     7981 ctggaagtaa cttttcttgt aataattttt ccataacctt ttcctcttaa atatatttta
     8041 ttatcaaaag ttatcaatta tctttatttt ttgacgtcaa gttgttattt gattatcttt
     8101 tgttatttat attttacaac tataagaaag ccttgtcaag gatttgtgta aacatttgat
//
LOCUS       DAVKLW010000083         7252 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKLW010000083 DAVKLW010000000
VERSION     DAVKLW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.083"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(162..965)
                     /locus_tag="VW324_001921"
     CDS             complement(162..965)
                     /locus_tag="VW324_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9727482.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(1049..1261)
                     /locus_tag="VW324_001922"
     CDS             complement(1049..1261)
                     /locus_tag="VW324_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727483.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(1740..1949)
                     /locus_tag="VW324_001923"
     CDS             complement(1740..1949)
                     /locus_tag="VW324_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727484.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            2119..2391
                     /gene="rpsP"
                     /locus_tag="VW324_001924"
     CDS             2119..2391
                     /gene="rpsP"
                     /locus_tag="VW324_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9727485.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            2411..2650
                     /locus_tag="VW324_001925"
     CDS             2411..2650
                     /locus_tag="VW324_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9727486.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            2918..3122
                     /locus_tag="VW324_001926"
                     /pseudo
     CDS             2918..3122
                     /locus_tag="VW324_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            3226..4014
                     /locus_tag="VW324_001927"
     CDS             3226..4014
                     /locus_tag="VW324_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9727487.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            4045..4563
                     /gene="rimM"
                     /locus_tag="VW324_001928"
     CDS             4045..4563
                     /gene="rimM"
                     /locus_tag="VW324_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9727488.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            4553..5272
                     /gene="trmD"
                     /locus_tag="VW324_001929"
     CDS             4553..5272
                     /gene="trmD"
                     /locus_tag="VW324_001929"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9727489.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGSQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKILVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            5284..5622
                     /locus_tag="VW324_001930"
     CDS             5284..5622
                     /locus_tag="VW324_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727490.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(5653..5973)
                     /locus_tag="VW324_001931"
     CDS             complement(5653..5973)
                     /locus_tag="VW324_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727491.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            6071..6286
                     /locus_tag="VW324_001932"
     CDS             6071..6286
                     /locus_tag="VW324_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9727492.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(6355..6435)
                     /locus_tag="VW324_001933"
                     /pseudo
     CDS             complement(6355..6435)
                     /locus_tag="VW324_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6491..7027)
                     /locus_tag="VW324_001934"
     CDS             complement(6491..7027)
                     /locus_tag="VW324_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9727493.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT         2343 a         1246 c         1503 g         2160 t
ORIGIN      
        1 tctttctaat gagttgtttt gtcgcttttc attataggtc atatgggact ttttttctac
       61 aataaaatag gctccataat atctatagtg gatttaccca ctatagatat tatagagcca
      121 gaaatacagt accgaatgtg gatactgtat ttttttggtt tttaagattt aaaatcgtag
      181 tctttcacca cttcgatgct gtcgattgtg atagcagtag ttggcttgtc tttttcatct
      241 ttttcggctt tagcaatctt atccacaaca tccataccgt caatcacttg accaaagact
      301 gggtgtttgc catctagact agggtttcca ccttctttgt aggcttcgat aattttctgt
      361 ggatacttgc ttgtagggag tttagaagag gtatctgtag agttttggtt gatgaagaac
      421 tggctgccat tggtgtttgg ttgaccagta ttagccatag caagagcacc acggatgtta
      481 tacaaataag gagtaatctc gttcttgaaa ccagttcctt tgtctttagt cttatccttg
      541 tcatgccata tggactgacc acctgtaccg tccccttttg gatctccagt ttggaccata
      601 aagccatcga tgacacggtg gaaggtaata ccgttatagt agccttcttt ggcgtgagtg
      661 aggaaatttt caaccgctag aggagcgagt ttagggaaga gtttaatgcg aatatcacct
      721 tggcttgtgt ggagaatcac ttcggcttca tcttcagcaa cttccttaga tagttgtggg
      781 aaattggcgt tttcgtttgt taaagcatcg tttaactcct tggcagattg ggcagctact
      841 ttggaacttt cttcagcagc aagactggaa tcaacataat catcaccacg cagactgcgt
      901 tggacgctgc tacacccagc tagggctaca gtagacagta aaagaagggt tgctagtttt
      961 ttcattctga tcctctcaaa aattcattta taaccattgt accctaaaag gaagtggatt
     1021 tcaaatatta gtagtttaca gaactggctt agataatagt tgggatttca tcatcatctt
     1081 gaaaatcttc aacttcttcc ccagtaaatg gtttctgaaa agaacttagt ataaaacggg
     1141 caattggttc aacaattagg aaatttagag gaagagctgc aataaagttt acgaaccagg
     1201 cgcttagata ggtattgggt gtgatagcaa tgccattgta aaaaagtcca tagaaggaca
     1261 tgaaagtgac cagctaattc ttcttctgaa aaatgctcat gaagggcttg atccaggtcg
     1321 caagatttca ttttagaaag tccagtaact cccttcttct tcaggatatt ctttttcatg
     1381 gtagtattta gatggtcaag tgagtcaaaa gaagtttcga caagagcata ccctttttct
     1441 accagagttt ttagatgttt aggggcatca ataccataag tgtactcaaa gtattttgga
     1501 aaccagatct taagaaagct cgtaaagcta gccagttctc taaacgctaa gaactagcta
     1561 caacacagca tttacaacac ttcatggttc acaccatggg gtgtttttta atgattttaa
     1621 gcaaaattca cacttaattg aaagttattt aaattagatg aaaatttatt ttttgaaaaa
     1681 gtgggaaaac attgattcta agcgaatttt gaatcatatt cgaatgggag tttttaaaat
     1741 catgtttcag tggtaaaagt accaaactgg attctccata gtaaaatgat atctcctgct
     1801 agtgaatcat ctactaataa gtccatattt cgcttgggaa cgggcttggg acttagaagc
     1861 caagtatcca actctcgttc aggcgatatg aagggtttga cttggtgttc tttataaaat
     1921 ttttctaaaa tagtattcac ggatagcatc tttcaaacat ttatttccta ttctaacaaa
     1981 tttctaaata ctttaagaaa tttatagtct gtcaagtttt tttcttgaca catgccagaa
     2041 atctgctata atagaacatg tgctagatag ctcagctatt tcaccgaaat aaaaaattaa
     2101 agaaatgaga ctttaaaaat ggcagttaaa atccgtttga ctcgtatggg ttctaagaaa
     2161 aaacctttct accgtatcaa cgtagcagat tcacgttcac cacgtgacgg acgtttcatc
     2221 gaaacagttg gaacttacaa cccacttgtt gctgaaaacc aagtaacttt gaaagaagac
     2281 cgcgttcttg catggttggc taatggagct caaccttcag acacagtacg taacatcctt
     2341 tcaaaagaag gcgtattgaa aaaattccac gattctaaat tttcaaaata agtttaaagt
     2401 aggttgacag atggatacga ttgaaaatct cattattgcg attgtgaaac ccttaatttc
     2461 acaaccagat gccttaacta tcaagattga ggatacacca gaatttttgg aatatcattt
     2521 gaatcttgat caaagcgatg tgggtcgtgt aatcggtcgt aagggtcgca ctatttctgc
     2581 gataagaacg attgtctact ctgtcccaac tgaatacaaa aaagtaagaa tcgttattga
     2641 cgaaaaataa gaagggcggg acggatgtct cgcttttttg tgtggaatag aagatatagt
     2701 aaaatgaaat aataacagga caaatcgatc aggacagtca aatcgatttc taacaatgtt
     2761 ttagaagtaa aggtgtacta ttctagtttc aatatactat aaaagattga acgtttaggc
     2821 aattccaaga ctacttactc gaacattaac agcagtctcg gactgatgaa ggtctcttta
     2881 tgaagttagt ggagaagtcg gagaagtagc cgaggttttg aataggcgct ctggtcgaaa
     2941 agaagggata ggattcgaac gaggaacttg ccagagaact ggctgatatc atttactaca
     3001 ccgtcgcaat cgtagctatt aatcatattg accttaccaa gactgtcttt gagaaagaca
     3061 aaaccgcagc cgttaattac caacataaac atgatttgga aggatttttg aagggaaagt
     3121 agagatgttg tccccttcct ccaacttgag aaaatccttg gaacgtgata aaataaaaga
     3181 taaggatttt taataattca ttatatagaa tgagtggatt tctttatgaa gaggtttgaa
     3241 gtatctacag aaattggtag cctctctgtt acttatcaaa agcaaaagaa agtgctagtt
     3301 tgtctaaacg gagcaggttt gctaccaagt tatgaaaatt tttcacttat acttgaaaaa
     3361 cctcctccta caattggtta tttgacaatt gattttccga acacaggtag gagtccgatt
     3421 catgaccaag ctggaaaaaa tctagataat cttgcagatg tggtttatga agtacttgaa
     3481 gaattaggga tttctgaata tatactttgt gcacatagtt ggagtggaat tttagcttgc
     3541 aaattactcg agaaaccaat taagtgtcag actttagtag caattgaacc gacaactaaa
     3601 aaagtcatgt ttgctgattt ttcagaaaat ccttatccag aaatggaaga gcagatgagg
     3661 ctgattgacg agtgtggtcc tgaactttat tttaagaact taactcaagc aacatttagt
     3721 cctgaaacga ataaaaaaat ctgggaatta atgcaagaaa aaggcttaga gttggaaaat
     3781 caagatccag aatttcagat atctggagag attactgagg aagattttga gaatttgtcg
     3841 atagaatctc atgtccctgt atttattttt tgtcagactt atagagaaaa agagtacaga
     3901 gaatcagaat attggacttc taatactaaa ctcattttag gagggaatca ccattattta
     3961 caatggtcag aatcggaaaa aattgcggct attattcgag aattgtcaga ataagatgga
     4021 aaaaaggaga ttacaggaga caagatgaac tactttaatg ttgggaaaat cgttaatacg
     4081 cagggattac agggtgagat gcgagtcttg tctgtgacgg attttgcaga agaacggttt
     4141 aaaaaaggag ctgagctggc tttgtttgat gaaaaagatc agtttgtcca aacagtgacc
     4201 atcgctagcc accgtaaaca gaagaacttt gacattatta aattcaaaga tatgtaccat
     4261 atcaatacta tcgaaaagta caagggatat agtctcaagg tcgctgagga agatttgaat
     4321 gacctagacg atggtgaatt ttactatcac gagattatcg gtttggaagt ctatgagggt
     4381 gatagcttgg ttggaaccat caaggaaatc ctgcaaccag gtgctaatga tgtctgggtg
     4441 gtcaaacgaa aaggcaaacg tgatttgctt ttaccttata tcccaccagt ggttctcaat
     4501 gttgatattc caaataaacg ggtcgatgtg gaaatcttag aagggttaga cgatgaagat
     4561 tgatatttta accctctttc cagagatgtt ttctccactg gagcactcaa tcgttggaaa
     4621 ggctcgagaa aaagggctct tggatatcca gtatcataat tttcgagaaa atgctgaaaa
     4681 ggcccgtcat gtagatgatg agccctacgg aggcagtcaa ggcatgttgc tcagagcaca
     4741 acctattttc gattcctttg atgctattga aaagaaaaat ccgcgcgtta ttctcctcga
     4801 tcctgctgga aagcagtttg atcaggctta tgctgaagat ttggctcaag aggaagagct
     4861 aatctttatc tgtgggcact atgagggtta tgatgagcgc attaagatct tggtaacaga
     4921 tgagatttcc ctaggcgact atgtcctcac tggtggagaa ttggcagcta tgaccatgat
     4981 tgatgctaca gttcgcctga ttccagaagt gattggcaag gagtctagcc accaagatga
     5041 tagtttttct tcaggtcttt tagaatatcc tcagtacaca cgtccctatg attatcgagg
     5101 catggtcgtg ccagatgtat tgatgagtgg ccaccatgaa aagattcgtc agtggcgatt
     5161 gtacgagagt ttaaagaaaa cctacgagcg cagaccagat ttacttgaac attatcaact
     5221 gacagtagaa gaagaaaaaa tgctggcaga aatcaaagaa aacaaagaat aaaggagaaa
     5281 cctatgcaag taatcaaacg taatggcgaa attgctgaat ttaatccaga taagatttac
     5341 caagccatct tgaaggcagc ccaaactgtt tatgttttga cagatgattt gcgtcaaaat
     5401 cttgctcaag tcactaagaa ggtagttttg gatttacaag aagccaaggt ggaacgtgcg
     5461 actatcagta tgattcaatc tatggttgaa catcgtttat tgggcgcagg ttacattacc
     5521 attgcagaac actacatttc ctatcgttta caacgtgact tggaaagaag tggttatgga
     5581 gatcatattg cagttcattt acattttgaa caaattcgct aagaaaaaag agtgggatga
     5641 agcaactaca tctcactctt tcttaaatct atttttttgg gctgtttgga cagttgaggg
     5701 gacaaatcgc aggcgttgaa tatcgcttat acggataatc cggctcacat ttttggcaaa
     5761 atttttcacg ataatttgct ggccttgctg atcatatttg atgatatcac ctgtaaaact
     5821 tgtctcagac aagataaggt gaacagcggt ttgtttctgg atagcctctt caatagtggc
     5881 tgtaagggag ttggtttcag tctggtcagg ttggtcatgt ccatttaaaa aatcatgtat
     5941 tttatctaga acttgctgga atttatgtct catattggcc tcccttcttt tatatcaata
     6001 ttatataaaa ttttcctaaa atctacaaga ttttacgaat aaactaatga aactaaaaaa
     6061 ggagaagaaa atggcagaat ttacatttga aatcgaagag cacttgttga ctctttctga
     6121 aaacgaaaaa ggttggacca aggaaattaa ccgtgtgagc tttaatggtg cccctgcaaa
     6181 gtttgatatt cgtgcttgga gtccagacca tactaaaatg ggcaaaggga ttactctctc
     6241 aaatgaagaa tttcaaacga tggtggatgc ctttaaaggc aactaatact cttcgaaaat
     6301 ctcttcaaac cgcatcagct ttgccttgcc gtatgtatgg ttactgactt cgtcagtttc
     6361 atccacaacc tcaaaacagt gttttgagca gcctgcggct agcttcctag tttgctcttt
     6421 gattttcatt gagtataagt tttcaaaata aaaaaaagga tattgagtca tgataactcg
     6481 gtatcctttt ttagttagta aagtaagcct gatttttaag gcgttgaagt aggggacggc
     6541 taataaaact aatagccaga agtttgccaa ggtctggaat gataaaggga agaaccccca
     6601 caacaagagc tttttcaaat gccattccag ctaggaaatg caagctgaga atcccgccga
     6661 caaagacaag ggcatcaccc aagaggtttg caagaaaaat cttaacaaca ccactcttgc
     6721 tgttggttag agaggaagta agtccagagt aaacgagata aaaccaaaga tagcctgcag
     6781 tagggccaac taaagcctga aaaccagctc cacctcctgc aaagacagga agaccgatag
     6841 cacctagaag aagatagagt ccagcagaaa gtacagcctc tctcggtcta aagacagtag
     6901 caatcaagcc gattgcaaag ttttgcagag tgaagggaac aggtccaatt ggaagactga
     6961 tttgtgccaa aacagcaatg agagcagccc caatagcagg gatagcataa acgtgagctt
     7021 ttttcaaaat agttaacctc ttttctaatg tatagtaact attatactca tataggagtc
     7081 gatgtcaacc attttttaaa aacaaggtaa caaaaactgt aacagccgat tttcgtgact
     7141 agtcaagttg gataattttt ttgttgattc gaaaattgtc tgtatagata atcctatttt
     7201 attaataaaa aatatactag taaatacttg acaaattaaa aaataagtgt ta
//
LOCUS       DAVKLW010000084         7161 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKLW010000084 DAVKLW010000000
VERSION     DAVKLW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.084"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..255
                     /locus_tag="VW324_001935"
     CDS             <1..255
                     /locus_tag="VW324_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9727494.1"
                     /translation="HIYYAHPYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEK
                     WINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRFQ"
     gene            330..530
                     /locus_tag="VW324_001936"
     CDS             330..530
                     /locus_tag="VW324_001936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9727495.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            565..1281
                     /gene="atpB"
                     /locus_tag="VW324_001937"
     CDS             565..1281
                     /gene="atpB"
                     /locus_tag="VW324_001937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9727496.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1295..1789
                     /gene="atpF"
                     /locus_tag="VW324_001938"
     CDS             1295..1789
                     /gene="atpF"
                     /locus_tag="VW324_001938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9727497.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            1789..2325
                     /locus_tag="VW324_001939"
     CDS             1789..2325
                     /locus_tag="VW324_001939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9727498.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2340..3845
                     /locus_tag="VW324_001940"
     CDS             2340..3845
                     /locus_tag="VW324_001940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9727499.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            3861..4739
                     /locus_tag="VW324_001941"
     CDS             3861..4739
                     /locus_tag="VW324_001941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9727500.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            4825..6231
                     /gene="atpD"
                     /locus_tag="VW324_001942"
     CDS             4825..6231
                     /gene="atpD"
                     /locus_tag="VW324_001942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9727501.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6242..6661
                     /locus_tag="VW324_001943"
     CDS             6242..6661
                     /locus_tag="VW324_001943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9727502.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(6733..>7161)
                     /locus_tag="VW324_001944"
     CDS             complement(6733..>7161)
                     /locus_tag="VW324_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9727503.1"
                     /translation="RKLSDKHFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLL
                     FHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLPYSNAKLEATNN
                     LIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         2143 a         1327 c         1578 g         2113 t
ORIGIN      
        1 cacatctatt atgcgcaccc ctatgcctct tgggaaaggg gaactaatga gaatcacaac
       61 aggctcattc gtagatggtt acctaaggga accaagaaaa tgactcccaa agaagtcgca
      121 ttcatcgaaa agtggattaa caactatcct aaaaaatgct tggactacaa gtcacccaga
      181 gaagacttct ggatggctaa cttgaacttg aaatttagca aaatggaaat aatttttatt
      241 aaacgcttcc aatagcggtt aaaagttgac aaatttaaat tttaggacta cactaagtaa
      301 gtaaatttta ttttaaagga gaatttgtta tgaatttaac atttttaggc ttatgtattg
      361 cctgtatggg cgtatctgtc ggtgaaggtt tattgatgaa tggactgttt aaatcagtag
      421 cacgccaacc agatatgctt tctgagtttc gtagtttgat gtttttaggt gttgccttta
      481 ttgaaggaac tttctttgta actcttgtct tctcatttat tatcaaataa atacatggaa
      541 cgagaagaaa agggaggatt ttagatggaa gaaagtatta atccaatcat ctctattggt
      601 cctgttatct tcaatctgac tatgttagcc atgactttgt tgattgtggg agttattttt
      661 gtctttattt attgggcaag ccgcaatatg accttgaaac ccaaaggaaa gcaaaatgta
      721 cttgagtatg tctatgactt tgttattgga tttacagaac ctaacattgg ttcgcgctac
      781 atgaaagatt actcactctt tttcctttgt ttattccttt tcatggtgat tgccaataac
      841 cttggcttaa tgacaaagct tcaaacgatc gatgggacta actggtggag ttcgccaacc
      901 gctaatttac agtatgactt aaccttatct tttcttgtca ttttgttgac acatatagaa
      961 agcgttcgtc gtcgtggatt taaaaaaagt ataaaatctt ttatgagtcc tgtttttgtc
     1021 ataccgatga atatcttgga agaatttaca aacttcttat ctttggcttt gcggattttt
     1081 gggaatatct ttgcaggaga ggtcatgacg agtttgttac ttcttctttc ccaccaagct
     1141 atttattggt atccagtagc ctttggagct aatttggctt ggactgcatt ttctgtcttt
     1201 atttcctgca tccaagctta tgtttttact cttttgacat ctgtgtattt agggaataag
     1261 attaatattg aagaggaata gaaaggagta actgatgcac gtaacagtag gtgaattaat
     1321 tggtaatttt attttaatca ctggctcttt tattcttttg ctagtcttga ttaaaaaatt
     1381 tgcatggtct aatattacag gcattttcga agaaagagct gaaaaaattg cttcagatat
     1441 tgacagagct gaagaagccc gtcaaaaagc agaagtattg gctcaaaaac gcgaagatga
     1501 attggctggt agccgtaaag aagctaagac aatcattgaa aatgcaaagg aaacagctga
     1561 gcaaagtaag gctaatatct tagcagatgc taaactagaa gcaggacact taaaagaaaa
     1621 agccaatcaa gaaattgctc aaaataaagt agaagcttta cagagtgtta agggtgaggt
     1681 cgcagatttg accatcagct tagctggtaa aatcatctca caaaaccttg acagtcatgc
     1741 ccataaagca ctcattgatc agtatatcga tcagctagga gaagcttaat ggacaagaaa
     1801 acagtaaagg taattgaaaa atacagcatg ccttttgtcc aattggtact tgaaaaagga
     1861 gaagaagacc gtatcttttc agacttgact caaatcaagc aagttgttga aaaaacaggt
     1921 ctgccttctt ttttaaaaca agtggcagta gacgagtcgg ataaggaaaa aacaattgct
     1981 tttttccaag attctgtgtc gcctttatta caaaacttta tccaggttct ggcctacaat
     2041 cacagagcaa atctttttta tgatgtgctt gtagattgct tgaaccgact tgaaaaagaa
     2101 acaaatcgat ttgaagtgac gattacgtct gctcatcctc taactgatga acagaagact
     2161 cgtttgctcc ctttgattga gaaaaaaatg tctctgaaag taaggagtgt aaaagaacaa
     2221 atcgatgaaa gtctcattgg tggttttgtc atttttgcca atcacaagac aattgatgtg
     2281 agtattaaac aacaacttaa agttgttaaa gaaaatttga aatagaaagt ggtgttcttt
     2341 tggcaattaa cgcacaagaa atcagcgctt taattaagca acaaattgaa aatttcaaac
     2401 ccaattttga tgtgactgaa acaggtgttg taacctatat cggggacggt atcgcgcgtg
     2461 ctcatggcct tgaaaatgtc atgagtggag agttgttgaa ttttgaaaac ggctcttatg
     2521 gtatggctca aaacttggag tcaacagacg ttggtattat catcctaggt gactttacag
     2581 atatccgtga aggcgataca atccgccgta cagggaaaat catggaagtc cctgtaggtg
     2641 aaagtctgat tggtcgtgtt gtggatccgc ttggtcgtcc agttgacggt cttggagaaa
     2701 tccacactga taaaactcgt ccagtagaag caccagctcc tggtgttatg caacgtaagt
     2761 ctgtttcaga accattgcaa actggtttga aagctattga cgcccttgta ccgattggtc
     2821 gtggtcaacg tgagttgatt atcggtgacc gtcagacagg gaaaacaacc attgcgattg
     2881 atacaatctt gaaccaaaaa gatcaagata tgatctgtat ctacgtcgcg attggacaaa
     2941 aagaatcaac agttcgtacg caagtagaaa cacttcgtca gtacggtgcc ttggactaca
     3001 caatcgttgt gacagcctct gcttcacaac catctccatt gctcttccta gctccttata
     3061 ctggggttgc tatggcggaa gaatttatgt atcaaggtaa gcatgttttg attgtatacg
     3121 atgatctatc aaaacaagcg gtagcttatc gtgaactatc gctcttgctt cgtcgtcctc
     3181 caggtcgtga agccttccca ggggatgttt tctatctcca cagccgtttg cttgagcgct
     3241 cagctaaagt ttctgatgaa cttggtggtg gatcaattac agccctacca tttatcgaga
     3301 cacaagcagg agatatctca gcctatatcg caaccaacgt gatttctatc actgatggac
     3361 aaatcttcct tggcgatggc ctcttcaatg caggtattcg tccagccatc gatgcgggtt
     3421 catctgtatc tcgtgtaggt ggttctgcac aaatcaaagc catgaagaag gttgctggta
     3481 cacttcgtat cgaccttgct tcataccgtg agttggaagc ctttactaag tttggttctg
     3541 acttggacgc agcaacacag gctaagttga accgtggacg tcgtaccgtt gaggtcttga
     3601 aacaacctgt tcacaaacca ttacctgttg agaaacaagt aaccattctt tatgctttga
     3661 cacatggttt cttggatact gttccagtag atgatattgt tcgtttcgag gaagagttcc
     3721 atgccttctt tgatgctcaa catccagaga ttttggaaac cattcgtgat acaaaagact
     3781 tgccagaaga agcagtcttg gatgctgcga ttacagagtt tctcaatcaa tctagcttcc
     3841 aataagaata gaggtgtcag atggcagtgt ctctaaatga tattaaaaca aaaatcgcct
     3901 caacaaaaaa tacgagtcaa atcactaatg ccatgcaaat ggtatcggct gctaagttag
     3961 gtcgctctga agaagctgct cgcaacttcc aagtttacgc tcagaaagtg cgtaaacttt
     4021 tgacagatat ccttcatggt aatggagctg gtgcttcaac taatccgatg ttgattagcc
     4081 gttctgtgaa gaagacaggc tatatcgtta tcacttcaga ccgcggtttg gttggaggtt
     4141 ataattcctc tattttgaaa gctgttatgg agttgaaaga agaataccac ccagacggta
     4201 aaggttttga aatgatctgt atcggtggga tgggagctga tttctttaag gctcgcggta
     4261 ttcaaccact ttatgaatta cgtggcttgg cagaccaacc tagctttgat caagttcgta
     4321 agattatttc aaaaactgtt gaaatgtacc aaaatgaact ctttgatgag ctttatgttt
     4381 gctacaacca ccatgtcaat acgctaacca gtcaaatgcg tgtggaacaa atgcttccga
     4441 ttgttgactt ggatccaaat gaagcggatg aagagtacag cttgactttt gaattggaaa
     4501 ccagccgaga agaaattctg gagcagttgt tgcctcagtt tgcagaaagt atgatttacg
     4561 gtgccattat cgatgccaag acagctgaga atgctgcggg tatgacagcc atgcaaacag
     4621 cgacagataa tgctaagaaa gtcatcaatg atttgacaat tcagtataac cgtgccagac
     4681 aggcggcgat tacacaagaa attacagaaa tcgtagcagg tgctagtgcc ttagaatagg
     4741 ctctagtcca gctcgtatga aaatgaactt aggacctagt tgagctagga accgacagta
     4801 tcttatatag aataggagaa ggagatgagt tcaggtaaaa ttgctcaggt tatcggtccc
     4861 gttgtagacg ttttgtttgc agcaggggaa aaacttcctg agattaacaa tgcacttgtc
     4921 gtctacaaaa atgacgaaag aaaaacaaaa atcgtccttg aagtagcctt ggagttagga
     4981 gatggtatgg ttcgtactat cgccatggaa tcaacagatg ggttgactcg tggaatggaa
     5041 gtattggaca caggtcgtcc aatctctgta ccagtaggta aagaaacttt gggacgtgtc
     5101 ttcaacgttt tgggagatac cattgatttg gaagctcctt ttacagaaga cgcagagcgt
     5161 cagccaattc ataaaaaagc tccaactttt gatgagttgt ctacctcttc tgaaatcctt
     5221 gaaacaggga tcaaggttat tgaccttctt gccccttacc ttaaaggtgg taaagttgga
     5281 cttttcggtg gtgccggagt tggtaaaacc gtcttaatcc aagaattgat tcacaacatt
     5341 gcccaagagc acggtggtat ttcagtattt actggtgttg gggaacgtac tcgtgagggg
     5401 aatgaccttt actgggaaat gaaagaatca ggcgttatcg agaaaacagc catggtcttt
     5461 ggtcagatga atgagccacc aggagcacgt atgcgtgttg cccttactgg tttgacaatc
     5521 gctgaatact tccgtgatgt ggaaggccaa gacgtgcttc tctttatcga taatatcttc
     5581 cgtttcactc aggctggttc agaagtatct gcccttttgg gtcgtatgcc atcagccgtt
     5641 ggttaccaac caacacttgc tacggaaatg ggtcaattgc aagaacgtat cacatcaacc
     5701 aagaagggtt ctgtaacctc tatccaggct atctatgtgc cagcggatga ctatactgac
     5761 ccagcgccag caacagcctt cgctcacttg gattcaacaa caaacttgga acgtaaattg
     5821 gtacaattgg gtatctaccc agccgttgac ccacttgctt caagctcacg tgccttggca
     5881 cctgaaatcg ttggagaaga gcactatgca gttgctgctg aagtaaaacg tgtccttcaa
     5941 cgttaccatg aattgcaaga tatcattgct atccttggta tggatgagct ttctgatgaa
     6001 gaaaagacct tggttgctcg cgcccgtcgt atccagttct tcttgtcaca aaacttcaac
     6061 gttgcggaac aatttactgg tcagccaggt tcttatgttc cagttgctga aactgtacgt
     6121 ggctttaagg aaatccttga tggtaaatac gaccacttgc cagaagatgc cttccgtggt
     6181 gtaggttcta tcgaagatgt gattgcaaaa gctgaaaaaa tgggatttta agaggtgacc
     6241 tatggctcag ttaactgtcc agatcgtgac accagatggt ctcgtctatg atcaccatgc
     6301 cagctatgta tcggttcgaa ctctggatgg tgagatgggg atcttgccac gacatgaaaa
     6361 tatgattgcg gttttagcag ttgatgaagt aaaggtaaaa cgtatcgatg ataaagatca
     6421 cgtgaactgg attgcagtaa acggtggcgt tattgaaatt gccaatgata tgatcacaat
     6481 cgtcgctgac tctgcagaac gtgctcgtga tatcgatatt agccgtgcag aacgtgccaa
     6541 acttcgtgca gaacgtgcaa ttgaagaagc acaagacaaa catttgattg accaagaacg
     6601 tcgtgctaag attgctttgc aacgtgccat taaccgtatt aatgtcggaa atagactata
     6661 agaaaaaatg aacttgaaaa taccaagttc attttttagg ctctttgtca actgtagtgg
     6721 gttgaaaaaa agctaagctc gagaaaggac aaattttgtc ctttcttttt tgatattcag
     6781 agcgataaaa atccgttttt tgaagttttc aaagttccga aaaccaaagg cattgcgctt
     6841 gataagtttg atgagattat tggtcgcttc caatttggcg tttgaatagg gtagttgaag
     6901 ggcgttgacg attttctctt tgtcctttag aaaggtttta aagacagtct gaaaaagagg
     6961 atgaacctgc ttaagattgt cctcaataag tccgaaaaat ttctctggtt ccttattctg
     7021 aaagtgaaac agcaagagtt gatagagctg atagtgatgt ttcaagtctt gtgaatagct
     7081 caaaagcttg tctaaaatct ctttattggt taaatgcata cgaaaagtag ggcgataaaa
     7141 atgtttatcg ctgagtttac g
//
LOCUS       DAVKLW010000085         6991 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKLW010000085 DAVKLW010000000
VERSION     DAVKLW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.085"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..230
                     /locus_tag="VW324_001945"
     CDS             <1..230
                     /locus_tag="VW324_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9727504.1"
                     /translation="KFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNE
                     FADFVVQVDKSKFACEIAIKVWRNSLKFSII"
     gene            260..3610
                     /locus_tag="VW324_001946"
     CDS             260..3610
                     /locus_tag="VW324_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9727505.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQVLLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(4116..4586)
                     /locus_tag="VW324_001947"
     CDS             complement(4116..4586)
                     /locus_tag="VW324_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9727506.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4603..6876)
                     /locus_tag="VW324_001948"
     CDS             complement(4603..6876)
                     /locus_tag="VW324_001948"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9727507.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFAYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2144 a         1186 c         1499 g         2162 t
ORIGIN      
        1 agaaatttat tatccatgtt ttaaggaata ataattatag tcgagtgata tcaggaagtg
       61 ctcagcctca gttaccaatt acaaaattaa aaaaaatact tctccccctc cccccactag
      121 ccctccaaaa tgagttcgca gactttgtag tccaggtcga caaatctaaa tttgcttgtg
      181 agatagctat aaaagtgtgg agaaatagct tgaaatttag tataatatag ctaaactatt
      241 tgtttaaagt gagaaaaaaa tgggaaattt tagctttctt ttaaaaaatg acgaatatga
      301 atctttttca aaaccttgca ttgaagctga gaatatgatt gctacatcaa ctgtggctac
      361 tgcctttatg gcgcgtcgtg ctttagagca ggctgtccat tggatatata gtcacgattc
      421 atatttagaa gctccctatc gtgctactct atcttcttta gtatgggatg atgattttag
      481 ggatatcgta gattctgaac tccacaagca gatagttctg ttgattcggt ggggaaacca
      541 tgctgctcat ggtggtgaaa ttaaggaacg agaagcgatt ttagctttgc atcatttgta
      601 tcagtttgtt aattttatcg attattgtta cagcaatgag tttgtggagc gttattttga
      661 tgagaagtgc ttaccacttt cagcaaacat caaataccga gaaactccac aatctatgat
      721 aaagttacaa gacagtttac cagaactgcc tgattttcat gaacagatgg ctgctcagtc
      781 cgtagaagtt caagagactt atactgaaaa acgtgagact gcagcgcaac ggcaagatgt
      841 gcctttccat attgatcaat tatctgaggc agagacaaga aagctcttta ttgatatcga
      901 tctccgttta gcaggatgga tatttgaaga aaactgtcgt gttgagatag ccgttgatgg
      961 tctcaagcac ggttcaggaa ttggttactg tgactatgta ctttatggta aaaatgggaa
     1021 aattttagcg attgtagagg ctaaaaaagc ctctgtcaat ccagaagtag gggaagtaca
     1081 ggtcaaagaa tatgctgaag ctcttgagaa acatatcggc tatcagccaa tttgctttat
     1141 tacaaatggc ttgaagcact atatacttga tggtccgaac cgccgccaga ttgcaggctt
     1201 ttactctcaa gaagaattgc aattagtgat ggatagacgt catcttcaaa aaccacttga
     1261 ggatatttct agtaaaatta gggacgatat ttccgggcgt cactaccaaa aacatgccat
     1321 tgcaagcgtt tgtgaagctt tctctaatca tcgtagacag gtacttttgg ttatggcaac
     1381 tggggctggg aaaactcgta cagcagtttc tctagttgat atcttatcac gtcataactg
     1441 ggtaaaaaac gttctcttct tagccgatag aacttccttg gttaagcaag cctatgattc
     1501 gtttagaaaa ttactcccag atctttccgt ttgtaacttc ttagaagata aagaaggagc
     1561 tcaatcaagt cgcatggtct tttcaactta tccgaccatg attggagcga ttagtggtca
     1621 agaagaagta aatcaacgcc ctttcactgt tgggcatttt gaccttatca taattgacga
     1681 atctcaccgt tctatttatc agaaatacaa gtccattttt gattattttg atgcaagaat
     1741 tgtaggctta acagctactc cgcgtcaaga tttagataaa aacacctatg gattctttaa
     1801 tttggagaat ggggttccaa catatgcata tgatttggaa gaggctgtta aagacggata
     1861 tttagtagcc tatcattcta tcgaaaccaa actgaaacta cctacggatg gtctacatta
     1921 tgatgatttg tccgaagaag aaaaggaaca ttttgatagc aaatttgaag acgatagctg
     1981 tgaaaaagat attgatggga gtgtatttaa ttcctttgtt ttcaataaaa gtacagtaga
     2041 aattgtttta aatgaactca tgacaagagg aattcagaca gcctcgggtg atgaaattgg
     2101 taaaactatt atttttgcta aaaatcatga tcatgcggaa tatatcagag gtatttttaa
     2161 caaccgctat cctgaaaaag ggagcgacta tgctcaggtg attgattata gtattaagca
     2221 ttatcagacc ttgattgatg attttaaaat taaggagaag tatcctcaaa ttgcgatttc
     2281 tgtcgatatg ttagatacag gtattgatgt accagaggtt gttaatttag tcttcttcaa
     2341 gaaagtacgc tctaaaacta agttttggca gatgattggt cgaggaaccc gtctatgtaa
     2401 agatttattt ggacctgagc aggataagga aaacttcttg gtatttgatt atggggacaa
     2461 ttttgattat tttcgtgcag atccaagaga tggagagggt cgtcacattg tttcgttgac
     2521 tcagcgttta tttaatatca aagtggactt gattcgagaa cttcagggac tccaatacca
     2581 agaagatcag tttgcgagag cataccgtca gcagcttgtc tcggaacttc aaggtcgtat
     2641 agagagctta aatgagttgg acttcagggt tcgtatggtt ttagatacag tttatagcta
     2701 taggaaattg gaaagttggc agaatctaac tgctgttaca agtgaaacca ttcaaaaaaa
     2761 tctctctctg cttttatttg atgaagataa agaagatgag atggcgagga gatttgattt
     2821 gtggttgctt catattcagt tggggcaact gacagctaaa tcttccactg ttcatatttc
     2881 ccaagtgatg aagacggcta gagctctttc tgctattggc aatatcccgc aggtttttga
     2941 gcaggctgaa attatcagga aagtacagga gcctgaattt tggaaagaag ttaacttgtc
     3001 tgatttggaa aaaattcgtc ttgctattcg agatttatta cagtttttgg ataaaacaga
     3061 ccgtaaaccc tactatgtta actttgaaga tcgtatactc tccactgttc acgagaccac
     3121 agcatttttg caggtcaacg atcttcggtc ttacaatgaa aaagttgagc attatttgaa
     3181 aactcatctg gatgaggagt ccatttctaa gctataccat aataaaaagt tgacatctga
     3241 tgatatgctt gcacttgaaa aattgctttg ggaaaaatta ggtagtaaag cagactacca
     3301 aagtcattat gaaaataagg caattccgag attggttcgt gagattattg gcttagatag
     3361 agagtctgcc aatcgtattt tttctaaatt tttgtcggat gagaatctta atgccaggca
     3421 gatttcattt gtaaaattga ttgtagacta cattgtagaa aatggttttt tagagacgaa
     3481 agtgttaacg caagagccgt ttaaatctta tggttctgtt caactactct tccaacacca
     3541 actaccagta cttcgtaata ttgttcaaat cattgaactt atcaataatc gagctggaga
     3601 agcggcttaa attctaaagt gattgccatg ctgagactca tttaaaatta aaaagagtag
     3661 aaatttatgc tatatatgag aagttttatt aggaagaatg tcatcgtttt cctagaatac
     3721 agtatcagtt gttaagtggt tgataaattt caaagtagat acttgtacca cgatgtttgt
     3781 tgatcgagtt attaacaaaa gagctacttt gattttaaag aaatagaaaa caaaaagccg
     3841 agcaagaatt caattgcagg agaaaatgaa ataatactca atgaaaatca aagagcaaac
     3901 taggaaacta gctgcaggct gctcaaaaca ctgttttgag gttgtagatg gaagctgacg
     3961 cggattgaag agattttcga agagtataaa tcttcctagg ataaagcaaa acgcatagta
     4021 tcaagggttt tcaacacttg atactatgcg ttttctgatg ttaaagactt tctaccaggt
     4081 tttttaaaag cataattgtt agttgtagtc atttattatt cttcaaagaa aaatggtggg
     4141 gcgaattttt tcagttcttc aaagcacttt tgagcagtat ctgcatcttc acagatgata
     4201 agacagacat cattaccaca aagggtagcg atagcgtcag ggaagctcaa agtatcaatg
     4261 atagaaccaa aggattgagc cagtccagga agggttttta gtaggacttg gtgttgaact
     4321 gggcgcatcc agacaagggc gtcttccatg tagagttcga gacgtttttc ccattttgag
     4381 atggaaccat tgttaagaac ataataagcg ctatcttctt cgcggacttt tgataggttc
     4441 atatttttga tgtcgcgtga gagggttgcc tgggttactt gaatgtcgtt ctcagcaaga
     4501 agggcttgca actcagcctg tgtatgaatc ttgttttttg tgataagagc gcgtataagt
     4561 tggtggcggt gttctgattt attcataata atgtaactcc ttttagcaag gtaaggtaag
     4621 catggactga gcgaggtcga cagtcaagtg gtagtctgta ttgtcacgga tggtgatttc
     4681 aaagtcagta gtatagagga ctaaacggag agtgtctcct tcttttagct tgtaaatagt
     4741 tggctgcagt tcaaattgaa cgtccatcca ttcatctgca gtaatatcct ctactaacag
     4801 taaatcattt ctattttgta aattaaggta accttttgtc acgactcgtt gtgcctctgg
     4861 tctaaatggc aattcacaga gattttccaa catgtgatag cgaccgttgt caatggttct
     4921 agcacttaaa atagctggat aaggttgtag gtatttcttt tgcccaaatt ctagcagttg
     4981 ggcagataag agccccttgt ttgtactgga tttgatacga agattgagct gagcgcgacc
     5041 gtttaggtgg agatctttag tcacaggaag gttaatagta atctgattgg ctttcccttg
     5101 atagagctct gtattgaagg tttggtatgt cttaccatag cgctcaaaat ccttatctgg
     5161 gtactggttt tgaatagctt gctcttcttg accaagtgag aaggtttcac agttttcttg
     5221 cccaccgaag ttatcaagtg ataaccaagt ctgtggagct gtattgtcct gccagataac
     5281 agtaggaagt tgaaagtctg tttcctgtcc tagtaatttc ttggtcaata aggcatttat
     5341 ggactcacgg aagtcaattg attgccaatt gttcatgtaa acatgggcac cattatggaa
     5401 aaagagatgc ttgtgtatat gagtaggaag agcatggaac atctggtaaa catgaagtgg
     5461 tttgacattc caatcctgag aaccatgagt aaagacaacc tctgccttta ctttatgggc
     5521 attgagcaga taattgcggt catgccaaaa ctgattgtag tccccagttt ttcggtctag
     5581 ctgagctttc actttttcta agtcagcttg gtgagcttca ttgccacgga tatagtcgcc
     5641 agctaagaga ttacgagaat aggttaactc agcaagggag tcaaagtcct cacctggata
     5701 accacctggg ctagtcacca gaccgttttc acggtagtag ttgtaccatg atgaaattcc
     5761 tgcctcggca atgataactt ctaaaccatc gactcctgta gtcgcaagac cattggacat
     5821 ggtacctaga taggaaagtc ctgttgtagc aacttttccg tttgaccaat cagccttgac
     5881 ttgacgctgg cgcgtgtgat cagtaaaggc acggcaacgg ccgttaagcc aatcgatgac
     5941 atttttataa gcctcgattt gctggtagtc tccattagtc atgaaacctg tcgagtcttt
     6001 ggtaccaaca cctgagacat agagattggc aaagcctctc ggaaggaagt agtcgtttag
     6061 tgtatagcta gagttgatgt gagttagctt ttcctcagcc tctgctataa gctcagcttt
     6121 accttggggt tggacgagat ttagttgagg tttctctagc tcaatcttgt gaggaagttt
     6181 aacctcaagc tcgccctcca tcttgtagag agccttgtca ctagccttgt cattggttcc
     6241 ctgatgataa gggctggctg tcatgatggc agggattttt ccatcaaaac gagggcgaat
     6301 aatgctaacc tttactaggt ctgatagccc tttttggtca gtatcgacac gagactcaac
     6361 gtaaacgact tcacgaatga catcctggtt agaaaaagta gccaaactct tgccgttaaa
     6421 gtagtggtag tcattatcct ccggaataag accatcacta acaagttggt cgataagagt
     6481 atttcctttt ttggtgcgag tattgagtaa ctgatagaga ttttcaatca agtcaccata
     6541 tataatggga aatccagttt ctttacgaaa aacgtcacta tcttcgaagt caaccaaata
     6601 agcaaagcct aaaagttgaa aagcaacagt ataaaaaata tctgctgtca gttcatcttc
     6661 tgattgaaaa aatgtcagca ggtctgtttt tttatcagct gctaggatag aaagtgggta
     6721 gttggtgtct tgataagtga aaaagaaacg acgtaaaaag gtttcaagtg agtctttgtg
     6781 attggctgta ttttgtaaat caaagccaca tttttttagt tcagataaga cattttcttt
     6841 tggaaaattg atataactat attgattaaa acgcatagaa cctccatata gaatgacagt
     6901 taaggttatt atatcaaaaa aaaagcagaa agggaattgt taacttcaaa aggaaataat
     6961 ccaataaaaa tgaataaagt actaaattca a
//
LOCUS       DAVKLW010000086         6901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKLW010000086 DAVKLW010000000
VERSION     DAVKLW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.086"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(97..372)
                     /locus_tag="VW324_001949"
     CDS             complement(97..372)
                     /locus_tag="VW324_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9727508.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(479..1318)
                     /locus_tag="VW324_001950"
     CDS             complement(479..1318)
                     /locus_tag="VW324_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9727509.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1459..2307
                     /locus_tag="VW324_001951"
     CDS             1459..2307
                     /locus_tag="VW324_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9727510.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2400..3317)
                     /locus_tag="VW324_001952"
     CDS             complement(2400..3317)
                     /locus_tag="VW324_001952"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9727511.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3755..3960
                     /locus_tag="VW324_001953"
                     /pseudo
     CDS             3755..3960
                     /locus_tag="VW324_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4271..4564)
                     /gene="rpmA"
                     /locus_tag="VW324_001954"
     CDS             complement(4271..4564)
                     /gene="rpmA"
                     /locus_tag="VW324_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9727512.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4581..4925)
                     /locus_tag="VW324_001955"
     CDS             complement(4581..4925)
                     /locus_tag="VW324_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9727513.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4941..5255)
                     /gene="rplU"
                     /locus_tag="VW324_001956"
     CDS             complement(4941..5255)
                     /gene="rplU"
                     /locus_tag="VW324_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9727514.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5291..5378)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5437..6141)
                     /locus_tag="VW324_001957"
     CDS             complement(5437..6141)
                     /locus_tag="VW324_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9727515.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6544..6843
                     /locus_tag="VW324_001958"
     CDS             6544..6843
                     /locus_tag="VW324_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9727516.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2171 a         1403 c         1150 g         2177 t
ORIGIN      
        1 ttcaactttt taactctatt tattactaga tttcataatt aaaaagccta ctgaccaagc
       61 tagaaagctt gatacaatag gctttttaaa gactgattat ttaacagcgt ctttaagagc
      121 tttaccagct ttgaatgctg gtactttaga agctgcaatt gtcatttctt taccagtttg
      181 tgggttgcga cctttacgtt ctgcgcgctc acgaacttca aagttaccaa aaccgatcaa
      241 ctgaactttt tcaccagctg caagatagtc agctactgct gcaaatacag cttcaactgc
      301 tgctgctgag tctttcttag tcaattctgt agcttctgct actttagcga tcaaatcttg
      361 tttgtttgcc atgttaatga ttcctccaaa taatttctaa ttaacaaata taatcatatc
      421 ctaaaatccc ttttaggtca agttaaaaac gctatttctt cccattttat ttattttttt
      481 aggagtggta acgtatcaaa atagcccaag cgttctcacc cgtgtgagtt tgaataatgg
      541 aacccgtttc caaaacagaa attggctttt caacataagc ttgtaagctt tctttcatct
      601 cttttgccca atcatcacta ccagaatatg aaattccaat ctctgctaca gcacgttcag
      661 aaagcgatgt tatcaactca tctaaccatt ttttaaatgt tttagttcca cgacctttaa
      721 ccattggctg caattcatgg tctttcattt gcatgacaac acggatattg agaagagagc
      781 tcaacaagcc agttacacgg ctaattcgtc cacctttgac aagattttcc aaagttgaaa
      841 caccaatata aagctctgta tggtttttaa cctcttctac atgagataaa attgcctcca
      901 tatctttacc ttcttgagct aacttcgcag cctcaacaac ttggaatttc agggcttggt
      961 cagtgaagga actatcaaca acagtcacat ctgcagtaga taggctagca ccttggcgtg
     1021 ctgcttctac cgtacccgaa agagcatggg acatatgaat agcaagaatc tggccaccat
     1081 ctttgcatag gtcttcaaaa atctcagcaa agacacctac aggtggctga cttgttttcg
     1141 gaagattctt actttcttgc atcaactgaa gaaatttacc ttcttctttc aaatccgcat
     1201 cagaataaac aacattatca atcattacag ataatggaac aattgtaata tctaattgct
     1261 ttactagttc aggttcaata gtaacagatg aatcggttac aatcttaatt tttgtcatag
     1321 tatcaatctt tctattttag gattcagatt ggtttcctta cttctaatta tatcaaaaaa
     1381 agattaaaaa tcctaatgga gtcaatcaaa ttttccgtaa aatttgatat aatcaactta
     1441 taagaaaaga ggtgtcctat gattaaaaaa atttacccca tttttaccat tttactaggt
     1501 gctgctattt atgcttttgg actgacttat tttgtagttc cccatcatct ctttgaagga
     1561 ggggcgacag gcattaccct catcaccttt tatcttttta aaatccctgt ttccctcatg
     1621 aacctgctga ttaatattcc ccttttcatc ctagcttgga agatctttgg agccaaatcc
     1681 ctctattcta gtttactagg aaccttagct ttgtccgcct ggttagcttt ttttgagcat
     1741 attccccttc atattgatct tcaaggtgat ttactaatca cagcccttat agcgggaatc
     1801 ctattgggaa ttggccttgg aattattttt aatgctggag gtacaactgg cggaactgat
     1861 attctagctc gtattctcaa caaatacact catatatcca taggaaaact gctctttatc
     1921 ttagattttt gtattctcat gttgattctc ctaatcttca aggatttgag attggtttcc
     1981 tacacgcttt tgtttgattt tattgtttct cgtgttattg atttgattgg tgaaggagga
     2041 tatgccggca aaggctttat gattatcaca aaacgtcctg accaacttgc taaggcgatt
     2101 aatgatgacc tcggaagagg tgttactttt atttctggtc aaggctacta tagtaaagaa
     2161 aatttgaaaa tcatctactg tattgtcgga agaaatgaaa ttgtgaaaac gaaggaaatg
     2221 attcatcgaa tcgatcctca agcctttata actattacag aagcccatga aatcctagga
     2281 gaaggcttca cctttgaaaa agaataaaaa gaggtaatgt cgtgacctca aaagttagac
     2341 taaatcatct atcttttggg ttacagacaa cctctttttt gttttattta ctcaagctct
     2401 taagaccaat tccgagttac ttcttcatca gcctttaact gatccactaa ttggtcaact
     2461 gagtcaaatt tggtcatatc acgaatgcga tcaagccaat aaaccatgac ggtttcccca
     2521 taaatatctt gattaaaatc aaaaatattg acttcaaaac gtgcttcttc tccatcaaag
     2581 gtcacatttt tcccgacact agccatagca cgatacttct gtctttgaat ctcaacatca
     2641 acgacataaa cgccatctgc tggcatataa gtacggtcta aaagcactaa attcgctgtc
     2701 ggataaccaa ttgtacgacc acgagcatta ccatgaacca ccatacctct tgatggaagc
     2761 ggtgccccca aaagttttcc tgcttctttc acatttccat ctaaaatagc ttgacggata
     2821 cgagttgaac taatctttcc tttctcatct tctacaggtg gaacaatgat aacttctcca
     2881 tcaaagtaat cctttaaatc ttctgctgtt tttttgtcag aaccaaatgt ataatcaaaa
     2941 cctgcaacaa taattttggc attcatagcc ttgatataag ttgcaaagaa ttcttgtgca
     3001 gtgagactag cgaattgact actaaaatca aggagatata attcttctac accttcgcgc
     3061 tttaattttc tttcacgttc agcagggttc aaaatatgca aaaacaaatc tggatgataa
     3121 ggctctaaag cgatctttgg agattcatta aaggtcataa cgacgatagg taataaatcc
     3181 tttctcgcag ccttgttggc aacacgaaat aattcttgat gccccttatg tatgccatca
     3241 aaatagccga gaacaacgac tgaatcagat ggtgtgccaa tatctttttg gttttttata
     3301 ggaatagtaa taatcataaa ataattatat catagcgata gctatttctg gaacagaaaa
     3361 tctgaaatgt tgtttttttc acatgaagtg tacctgtttt caaaaagcac tttattctat
     3421 cgttgcttaa ctatgaactt tgcaatattc ttctcaaaaa cttgtaggac atcttcaaaa
     3481 ttttgcaagg agtgattaga cttgttcggt aaccataaag tgtcatacta tgcttatgta
     3541 tgaaaaagca atgcaactaa ctcctgagaa ctttaaatta ctaattggtg ccgaaaaggt
     3601 agaatttaga atcgaggtac acctatggct gtaaaattta caaaatgaga caacttgggc
     3661 aagatgtttg aagaatttcc taaactccct gatttgaagc aagtcacttt ccctgatgac
     3721 aaagaaaaaa gccaaaacag caaagaaaaa ctagatgact gctttccaac aactcccatc
     3781 tagtgtgctt cagactaggc tatttttctc tccatctgtt agcttggatt ctcagaccgt
     3841 ttcagctaaa gaatatcttt tcccttatca gaaggaacgg ctcaaaccat tcagacaagt
     3901 gaagggacga caagccaata tttgaaacca gatagcagtt cttatagtca attgaaataa
     3961 aatctgaaga aatcgagtag gaaactcata tcaattttta acagtgttct attccagatt
     4021 catactcaat gaaaattaaa gtgcaaacta ggaagttagc cgcaggctgt acttgagtac
     4081 ggcaaggtga aattgacgtg gtttgaattt gattttcgaa gagtatcaat ccactataca
     4141 tattaaaaag tatgggtatc aaaataaaaa aacaggctct ccgaaaactc ggaaagcctt
     4201 attttaagct gtttctagct tcctcacctt tacatttcaa ggttcgggat aaaaggttca
     4261 atgaaccttt ttattttgcg attgggtaaa cagaaacttg ttttttatcg cgtcctttac
     4321 gttcaaagcg tactacgcct tcaactttag cgaacaaagt atcgtctcca ccacgaccaa
     4381 cgtttacacc tggatagatg tgtgtaccac gttgacggta aaggattgat ccacctgtta
     4441 cagtttgtcc gtcagctgct ttagctccaa gacgttttgc ttgtgaatca cgtccgtttg
     4501 atgtagaacc tccacctttt ttgtgggcga aaagttgcaa gttgttaaga gtcattttta
     4561 acataatgtt ttcctccgtg ttagttttct gtgataactc tggtttggac gaactcagaa
     4621 gagttctccg ataagtttgc catacctaag aaaaatgatt caaagaataa ctgggtcatt
     4681 tctctctggt gtgaaggaag atcttttggt atttcaacca tcagatagcc accttcatct
     4741 tcgtttaatt ctaggattgg ttcatagcct gcaaatttct caatagaatt gataaagtta
     4801 atggcaagcg tagaaaccga tgcacacacg acatctaagc cgtattcgcc actctcggcg
     4861 tgtccagtaa tttccgcact cctcagctcg ccatcttcgg ctctctcaaa gactgcctgt
     4921 atcatgtgtt ctccttaaaa ttaagcgttg attgcgttga tgacaacttt tgtatatggt
     4981 tgacggtgac cttgtttacg gtggctacct tttttaggtt tgtacttgta agtaaccact
     5041 ttcttttgtt ttccttgttt ttcaacagtt ccaactacag tagctccagc aacaagtgga
     5101 gttccgacaa cagtgttttc accaccaaca agaacaactt cgttaaaagt aacttcttga
     5161 ccagcttcaa cgttcaattt ttcaacgtaa actgcttgac caacttcaac tttaacttgt
     5221 tttccgccag ttttgataat tgcgtatgtg ctcattatgc acctcctatg atttttatgg
     5281 gtttccccga ttttttgtga agactcgcct agcatcgtgg gacgaaccac ttaaacttga
     5341 ataatcaagc aacgatgttc gtgcggttgc acaggatcgt gcatagtcaa ctcttcaagt
     5401 atagcatatc tcctattttc ttacaagtaa taacacctaa aatgaagctt tttcttttac
     5461 ttttttctgc caagaggcaa aaagcatgct gaggtaaaaa acgctcatca taataggaac
     5521 accaagaatg gtcttttcat gatagaaaat cgtcaaatag gctgaaaaga caacgccaag
     5581 gacaaaacta ctaagcaggc taacaaatat gaatccttca cgcaaaaaag gagtgtgctt
     5641 ggttcggaaa taatctccaa aagccagcat ggtccgtttg atattccctg tcataaaagc
     5701 gttattatag gcaatacccg acacttctcc aaaagcagtt gtcaccagtc ccatacagaa
     5761 ggccaagggc ggcactagat agatattatc cacagtttgc ggcacaaaag caataatgat
     5821 tgataagatt gccaagggaa tcaaggacag aataggtttt ttcacaattc tcaatttttc
     5881 cttataaatc gttaataaaa agactcccat cataaacgct agcaaggtga gaaccttgtc
     5941 cctaacatcc gaaacattat ttttaattaa ttctactgaa agaaagacaa catttccagt
     6001 ttgtccagct acaagggtat tcccgcgaac aataaaagtg taagcatcca catatccagc
     6061 acaaaacgtc aaaaaaagtg ctaacctttt agactgacgt gatatttttc ttataggtaa
     6121 taacctcatt ttacctccca ttgtattttc tcttagaaat attgtaccat tttctttcta
     6181 aaaaatcgta ggctaccatt tagattttac tattagcata aaaataataa tagacaacta
     6241 tttatccaaa aatagataga tgtaacatgt ttgcaaacaa agcatacgaa cctttagtaa
     6301 aatcatttcc atgaaactag aatagagccc tcttagcaaa aatcattatt ttaatttatt
     6361 tctaatcact ccttgacata aataactctc accaataaaa gactatgtct taaaaaaaat
     6421 ggtataataa aatcaatact tgggcttgat ggctatgcta ctaataacaa ttaggagaga
     6481 aaatcaggca cttgttaaca acaaggatta tccccttgag atgaaaggaa ctttagaaat
     6541 cttatgatga acatgcaaaa catgatgcgt caagcacaaa aacttcaaaa acaaatggaa
     6601 caaagccaag ctgaacttgc tgctatgcaa tttgttggca aatctgctca agatcttgtc
     6661 caagcgacct taactggcga taagaaagtt gtcagcattg atttcaatcc agctgtcgtt
     6721 gacccagagg accttgagac tctttctgat atgaccgttc aagccatcaa ctctgctctt
     6781 gaacaaatcg atgaaactac caagaaaaaa ctgggtgctt tcgctgggaa attacctttc
     6841 taaaaacaag gagctagaac aatgcttgtc gataacaaag gctaagaaag gtgcaaaaat
     6901 g
//
LOCUS       DAVKLW010000087         6800 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKLW010000087 DAVKLW010000000
VERSION     DAVKLW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.087"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..379
                     /locus_tag="VW324_001959"
     CDS             <1..379
                     /locus_tag="VW324_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9727517.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEEMMTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKK
                     HLKKVLPSCNTFYEALLSCSCFN"
     gene            465..1415
                     /locus_tag="VW324_001960"
     CDS             465..1415
                     /locus_tag="VW324_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9727518.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPISLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1592..2599
                     /gene="gap"
                     /locus_tag="VW324_001961"
     CDS             1592..2599
                     /gene="gap"
                     /locus_tag="VW324_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9727519.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2666..3502)
                     /locus_tag="VW324_001962"
     CDS             complement(2666..3502)
                     /locus_tag="VW324_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9727520.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3628..5823
                     /locus_tag="VW324_001963"
     CDS             3628..5823
                     /locus_tag="VW324_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9727521.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5876..6028
                     /gene="rpmG"
                     /locus_tag="VW324_001964"
     CDS             5876..6028
                     /gene="rpmG"
                     /locus_tag="VW324_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9727522.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6038..6214
                     /gene="secE"
                     /locus_tag="VW324_001965"
     CDS             6038..6214
                     /gene="secE"
                     /locus_tag="VW324_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9727523.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6269..>6800
                     /gene="nusG"
                     /locus_tag="VW324_001966"
     CDS             6269..>6800
                     /gene="nusG"
                     /locus_tag="VW324_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9727524.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2118 a         1187 c         1469 g         2026 t
ORIGIN      
        1 aagaggtaaa gtatctggaa gaagatatca gaggatttct ttggttgcag gtctaacaaa
       61 tggtgagtta atcgctccaa tgacttacga agagatgatg acgagcgact tttttgaagc
      121 ttggtttcag aagtttctct taccaacatt aaccacacca tcggttatta ttatggataa
      181 tgcaagattc catagaatgg gtaagttaga acttttatgc gaggagtttg ggcataaact
      241 tttacctctt cctccctact cacctgagta caatcctatt gagaaaacat gggctcatat
      301 caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc ttttatgagg ctcttttgtc
      361 ctgctcttgt ttcaattgac tatagttcac ggatacagtt gggaaagaag ctattaaata
      421 tttggagagt ccttttggga tgaacatgtc aaatattact atagatgggc aagaaaagat
      481 gggcaagaaa tggatctgag tcaaacgatg cttcttatgc tcaagtagtg agtctttatg
      541 atgatacaag tatctctgtt agtaataatg agacggacaa agtgttgact ggatccctct
      601 atactgaaac aaatgaacag ggattaacga ttccaatcag tttactaaaa aattggaatg
      661 aacagacagg aaaaaatttg acaactaatg atcttattgg caaatcagtc tcagccagca
      721 ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt tcaaacgaag attgtacgtg
      781 taatcaatga tgaagatgac atggaggaca gcaacagttt catgctgtct catcaaatgg
      841 aaacgatttt gaaagaggct ggatttacga aagctgtatc ttattttatc ttggaactca
      901 aagatccatc acagacaaaa gtagtaacag aagaattaca gaaaaataag aagtatactg
      961 tgctttctca acagagagtt cttgatattg tgattacctt tattcgtgtt attcagggat
     1021 tattgattgt gctttcatca caagctattg tggtagcagc ggttatgatt ggtatcatta
     1081 tttacatcaa tatcatgcaa cgttccaagg aaataggtgt catgaaagca gttggttatc
     1141 agaatcgtgg tgtcaaagga atttttattt acgaggctat ctggattgta ggcatcgcct
     1201 tgctgctggc atttttggtt gcacaagggg tgggaagttt ggcgaatgcg attgtaagtc
     1261 acttttaccc atccatcact aaggtttttg aattaaatct tttatctgtt ttaggaactc
     1321 tagttttcgc tctattactt ggttatgtct cagcctactt cccggcgcgt aagattagta
     1381 aaatggatcc tgtagaatcg ttacgctatg aataataagg gattccttga tttacagtct
     1441 taaaaagaaa ctcacataga aaaacaatgg ccatacttgc aattcaactc aaatagttgt
     1501 ataataggtt tgttgaaagg tgatttgtag tgattcaagt tactcttttc acaataaaaa
     1561 tttcatgatt ttcataagga ggaaatcact aatggtagtt aaagttggta ttaacggttt
     1621 cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac
     1681 acgcatcaac gaccttacag atccagttat gcttgcacac ttgttgaaat acgacacaac
     1741 tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt ggattcgaag ttaacggtaa
     1801 attcgttaaa gtttctgctg aacgtgatcc agagcaaatc gactgggcta ctgacggtgt
     1861 agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa gaagcagctg aaaaacacct
     1921 taaaggtgga gctaaaaaag ttgttatcac tgctcctggt ggaaacgacg ttaaaacagt
     1981 tgtattcaac actaaccacg acgttcttga cggtactgaa acagttatct caggtgcttc
     2041 atgtactaca aactgcttgg ctccaatggc taaagctctt caagataact ttggtgttgt
     2101 tgaaggattg atgactacta tccacgctta cactggtgac caaatgatcc ttgacggacc
     2161 acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct gcaaacatcg ttcctaactc
     2221 aactggtgct gctaaagcta tcggtcttgt aatcccagaa ttgaacggta aacttgacgg
     2281 atctgcacaa cgcgttccaa ctccaactgg atcagttact gaattggtag cagttcttga
     2341 aaagaacgtt actgttgatg aagtgaacgc agctatgaaa gcagcttcaa acgaatcata
     2401 cggttacaca gaagatccaa tcgtatcttc agatatcgta ggtatgtctt acggttcatt
     2461 gtttgacgca actcaaacta aagttcttga cgttgacggt aaacaattgg ttaaagttgt
     2521 atcatggtac gacaacgaaa tgtcatacac tgcacaactt gttcgtactc ttgaatactt
     2581 cgcaaaaatt gctaaataat tcttgagttg atagaaagca aggctttgtg gtcttgcttt
     2641 ttatatagaa aaatggatga cacgatcatc cattcttttt taattctttt tcaaatgtat
     2701 ttgaaagggt agtgaaagtt agcttttcta aagtaagtgg gtgggtaaag gaaagtcgga
     2761 aggcatgaag cataagccga cttgtctttg atttactatt atagagaggg tcgcccagga
     2821 taggaagatt atgatgcgaa aggtgcacac gaatctgatg ggttcgccct gtctttagct
     2881 tgcaacgagc caaggaagtc ttgtttgaga attgctttaa tctgcttaca tacgtttcag
     2941 catattgccc attttttgca tcaactattc tttttctacg atcatggcga tcacgtccaa
     3001 ttttgtctct gaaaacaagt tcttttctgt tgatatttcc atcaactaga gcccaatatt
     3061 ctctagaaat ctcttttttc tccaataagc gattgagaat gggcaggata aaaggatttt
     3121 tggcaaagag aactaagcca ctggtttcca tgtccagacg atgaacgaca tagcaggttt
     3181 ggccaacata ggtactgaca tggttaagaa gggcaatttc gtttggttga ttaccatgcg
     3241 ttttcatccc ctctggtttg tttacaataa tcaagtgttg atcttgataa acttcctgca
     3301 ctaagtctgg gttgccccaa gggatcgtct tttggggata atcttcctcg tcaaaagtca
     3361 actggcaaac atctccagga tttacgattt cgttccagtg gacttcttct tgatttatca
     3421 aaatatgttt cttgattctc aaaaaatgac ggatttttct agggatgagg agttgttcct
     3481 caagtaattg ctttaccgtc atttgaggta tagaggcggg taatgtaaat gtgaattgca
     3541 tacagatatt gtaacaaaaa aagccctatt tggataggaa atagctaaat tcttgtcttc
     3601 ctatgatgaa gatgataaaa taaacgcatg aaattagata aattatttga gaaatttctt
     3661 tctcttttta aaaaagaaac aagtgaacta gaggactctg attctactat cttacgtcgc
     3721 tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga ttcgaaaatt ctggcgtcgt
     3781 tatcatctaa caaagattat ccttatacta ggtttgagtg caggcttgct agttggaatc
     3841 tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt tgcaaaatgc cttgaaaact
     3901 cggactctta tttttgaccg tgaagaaaaa gaggctggtg ccttgtctgg tcaaaaggga
     3961 acctatgttg agctgactga catcagtaaa aacttgcaga atgctgttat tgcgacagaa
     4021 gaccgttctt tctataaaaa tgacgggatt aactatggcc gtttcttctt ggctattgtc
     4081 actgctggac gttcaggtgg tggctctacc attacccaac agctggctaa aaacgcctat
     4141 ttatcgcagg atcaaactgt tgagagaaaa gcgaaagaat ttttccttgc cttagaatta
     4201 agcaaaaaat atagtaagga gcaaattcta accatgtacc ttaacaacgc ttattttgga
     4261 aatggtgtgt ggggtgtaga agatgcgagt aagaaatact ttggagtttc tgcatcagaa
     4321 gtgagtctgg atcaagctgc gactctggca gggatgctca aggggccgga actgtataat
     4381 cccttgaatt ccgtagaaga ttctactaat cggcgcgata ctgtcttgca gaatatggtt
     4441 gcagcaggat atattgataa aaaccaagaa accgaagctg ctgaagttga tatgacttcg
     4501 caattgcacg ataagtatga aggaaaaatc tcagattacc gttatccctc ttattttgat
     4561 gcggtggtta atgaagctgt ttccaagtat aatctaacag aggaagagat tgtcaataat
     4621 ggctaccgca tttacacaga gctggaccaa aactaccaag caaatatgca gattgtttat
     4681 gaaaacacat cgctatttcc gagggcagag gatggaacgt ttgctcaatc aggaagtgta
     4741 gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg gtcaagttgc tgacaatgat
     4801 aaaactggat tccggaattt caactatgca acccaatcaa agcgtagtcc tggttctaca
     4861 attaagcctt tagttgttta tacaccagca gttgaagcag gctgggcttt gaataagcag
     4921 ttggataacc ataccatgca gtatgatagc tataaggttg ataactatgc agggatcaaa
     4981 acaagtcgag aagttcctat gtatcaatcc ttggcagaat cgcttaatct acctgctgtt
     5041 gccactgtta atgatttggg tgttgacaag gcttttgagg caggcgaaaa attcggactc
     5101 aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg gaagcggtgt tgaaaccaac
     5161 cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg aaggtttaat gcctgaagct
     5221 cattttatta gtagaattga aaatgctagt ggacaagtta ttgcgagtca taaaaattca
     5281 caaaaacggg tgattgataa gtctgtagct gacaagatga ccagtatgat gttggggact
     5341 ttcaccaacg gtaccggtat tagttcatcg cctgcagact atgtcatggc agggaaaact
     5401 ggaacaactg aagcagtttt caatccggag tacacaagtg accagtgggt aattggttat
     5461 actccggatg tagtgattag ccactggctt ggctttccga ccactgatga aaatcactat
     5521 ctagctggct ctacttcaaa cggtgcagct catgtcttta gaaacattgc caataccatt
     5581 ttaccttata cgccaggaag tacctttacg gttgaaaatg cttataagca aaatggaatt
     5641 gcaccagcca atacaaaaag acaagtacaa accaatgata atagccagac agatgataat
     5701 ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg aggctagccg ggctatctca
     5761 gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt cgatagtcaa tctatttcgc
     5821 taagatgctt gtcaaagcct agctttcttg ttataatgga taagatggag gcgttatggc
     5881 actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg agaaactatt caatcaagat
     5941 cagcggaaac cccaagccta cacgactaga agtaaataaa ttttgtaagc attgtggcaa
     6001 gtacactaca cacagagaaa cgagatagga gagagcgatg cgttttattg gagatatttt
     6061 tagacttctt aaagacacaa catggccaac tcgcaaggaa agctggagag attttcgttc
     6121 tatcatggaa tacacagctt tctttgtagt aattatttac atttttgacc agttgattgt
     6181 ttcaggtttg attcgattta ttaacatttt ttagaagatt agtggagtta attacactag
     6241 aaatcttcta tttatgaaag gaaatatcat ggatagtttt gataaaggat ggtttgtttt
     6301 acaaacttat tctggttatg aaaataaggt gaaagaaaat ctattacaac gtgcacaaac
     6361 ctacaatatg ttggataata ttctacgcgt tgaaattcca acacaaacag tgcaagttga
     6421 aaaaaatgga aagagaaaag aagtagaaga aaatcgcttt ccaggttatg ttcttgtaga
     6481 aatggtcatg acagatgaag cttggtttgt tgttcgaaac acaccaaatg ttacaggatt
     6541 tgtcggatca cacgggaaca gatcaaaacc aactccatta ttggaacaag aaattcgtga
     6601 cattttggta tctatgggac aaactgttca agaatttgat ttcgatgttg agattggtca
     6661 aactgtacgt attattgatg gtgcttttgc agactacact ggtaagatta cagaaattga
     6721 taataacaaa gtgaaaatga ttatctctat gtttggtaat gacacagttg cagaagtaaa
     6781 cctaaaccaa atcgcagaat
//
LOCUS       DAVKLW010000088         6091 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKLW010000088 DAVKLW010000000
VERSION     DAVKLW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.088"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VW324_001967"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VW324_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9727525.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1318..2022
                     /locus_tag="VW324_001968"
     CDS             1318..2022
                     /locus_tag="VW324_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9727526.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2015..3364
                     /gene="vicK"
                     /locus_tag="VW324_001969"
     CDS             2015..3364
                     /gene="vicK"
                     /locus_tag="VW324_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9727527.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3366..4175
                     /locus_tag="VW324_001970"
     CDS             3366..4175
                     /locus_tag="VW324_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9727528.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4266..4508
                     /locus_tag="VW324_001971"
     CDS             4266..4508
                     /locus_tag="VW324_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9727529.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4510..4764
                     /locus_tag="VW324_001972"
     CDS             4510..4764
                     /locus_tag="VW324_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9727530.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4839..>6091
                     /locus_tag="VW324_001973"
     CDS             4839..>6091
                     /locus_tag="VW324_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEU9727531.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLV"
BASE COUNT         1933 a         1013 c         1376 g         1769 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattc ctggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggcta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccggat cgtccgggtg
      661 attttaatca agcattgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ttggtcccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact atttttttgg tataatagta gagaaaaagg tgaacatatg
     1321 aaaaaaatac taattgtaga tgatgagaaa ccaatctcgg atattatcaa gtttaatatg
     1381 accaaggaag gttacgaagt tgtaactgct tttaatggtc gtgaagcgct agagcaattt
     1441 gaagcagagc aaccagatat tattattctg gatttgatgc ttccagaaat tgatggttta
     1501 gaagttgcta agaccattcg taagacaagc agtgtgccca ttcttatgct ttcagccaaa
     1561 gatagtgaat ttgataaggt tatcggtttg gaacttgggg cagatgacta tgtaacgaaa
     1621 cccttctcca atcgtgagtt gcaggcgcgt gttaaagctc ttctgcgtcg ttctcaacct
     1681 atgccagtag atggtcagga agcagatagt aaacctcaac ctatccaaat tggggattta
     1741 gaaattgttc cagacgccta cgtggctaaa aaatatggcg aagaactaga cttaacccat
     1801 cgtgaatttg agcttttgta tcatttagca tcgcatacag gtcaagtcat cacgcgcgaa
     1861 cacttgcttg agactgtctg gggttatgac tattttggtg atgtccgtac agttgatgtg
     1921 actgtacgac gtctgcgtga gaagattgaa gatacgccca gccgaccaga gtatatcttg
     1981 acgcgccgtg gtgtagggta ttacatgaga aataatgctt gatttactga aacaaaccat
     2041 ttttaccaga gattttatct ttatcctgat tttgttaggt ttcatccttg ttgtgaccct
     2101 cttattactg gaaaatagac gtgataatat tcagttgaag caaatcaatc aaaaggttaa
     2161 agatttgatt gcaggagatt attccaaggt tcttgatatg caaggtgggt ctgaaatcac
     2221 caatattacc aataatttga atgacttgtc ggaggttatt cgtctcactc aggaaaatct
     2281 agaacaagag agtaagaggc taaatagtat tctgttttat atgacagatg gggttcttgc
     2341 gaccaaccgt cggggtcaga ttatcatgat taacgataca gccaagaagc aactggggtt
     2401 ggttaaggaa gatgttctga atagaagcat tttggaattg ctcaagatag aagaaaacta
     2461 tgaattgcgt gatttgatta cccaaagtcc agaattgttg ctagattccc aagatatcaa
     2521 tggtgaatat ttgaaccttc gagttcgctt tgccttgata cgtcgagagt ctggctttat
     2581 ttcaggtttg gtggctgttt tgcatgatac gacggagcag gagaaggaag aacgcgaacg
     2641 aagactcttt gtttccaatg ttagccatga gttacggact cctctgacta gcgtaaaatc
     2701 ctatcttgaa gccttggatg agggggcttt gtgtgaaact gtagcaccag actttatcaa
     2761 ggtttctctt gatgagacca accgtatgat gcgcatggtg acggatctcc tccatctttc
     2821 acgtattgat aatgctacca gtcacctaga tgtggaactg attaacttca ctgcttttat
     2881 tacctttatc ctcaatcgtt ttgacaagat gaaaggacag gaaaaggaga aaaaatatga
     2941 gttggtgaga gattatccca tcaattctat ctggatggaa attgatacag ataagatgac
     3001 gcaggttgtc gacaatattt taaataatgc tattaagtat tcgccagatg ggggtaaaat
     3061 cactgtcaga atgaagacaa ctgaagacca gatgatttta tccatttctg accacggttt
     3121 ggggattcct aagcaggatt taccacgtat ctttgaccgt ttctatcgtg tggatcgtgc
     3181 tagaagtcgt gcacaaggtg gtacaggtct aggactgtct atcgctaaag aaattattaa
     3241 acaacataag ggctttattt gggccaagag tgaatacggc aagggttcaa cctttaccat
     3301 tgtgctccct tatgataagg atgcagtgaa agaagaagta tgggaggatg aagtagaaga
     3361 ctagaatgag tgaaataggc tttaaataca gtattttagc gtcgggttcc agtggaaatt
     3421 ctttttatct ggaaacctca aaaaagaagc ttttagtgga tgcaggcttg tctggcaaga
     3481 aaattaccag tctgctagct gaaattaacc gcaagccaga agacctggat gccatcttga
     3541 ttacccatga gcattcagat catatccatg gagtaggcgt tttggctcgc aagtatggta
     3601 tggatcttta tgccaatgaa aagacctggc aagctatgga aaatagtaaa tatcttggca
     3661 aggtggattc ttcgcaaaag catatttttg aaatgggtaa aaccaaaact tttggagata
     3721 tcgacatcga gagttttggt gtaagccatg atgcagtcgc accgcagttc tatcgcttta
     3781 tgaaggatga caagagtttt gtccttttga cagatacagg ttatgtcagt gaccgtatgg
     3841 cgggcattgt cgaaaatgcg gatggctatc ttatcgaggc caaccatgat gtagagattt
     3901 tgcgatcagg ttcttacgct tggcgactca aacaacgaat cctatctgac cttggtcacc
     3961 tttctaacga ggacggtgct gaagctatga ttcggacgct aggaaatcgc actaagaaga
     4021 tttaccttgg gcatttatct aaggaaaaca atatcaagga actggctcat atgaccatgg
     4081 tcaatcagct ggctcaagct gatctgggag tcggagtaga ctttaaggtt tatgatacct
     4141 caccagatac cgcaacacca ttgacagaga tatagaaaga acgctgagaa ggtgttcttt
     4201 ttatattgac tgaacaccta aaaagtaata caatggtgtt accattaaaa aagggagcac
     4261 aaaagatgac tactattaca ttaaaagttt ctgaagctga taaaacattt atgaaagcaa
     4321 tggctaagtt tgaaggagtt tccctttcgg aacttattcg caccaaaact cttgaagccc
     4381 tagaagatga atacgatgct cgtgtggcag atttagccta tcaagagtat ttagaagact
     4441 tggaaaaagg agttgaaccc attacttggg aagaaatgat gcatgattta ggcttgaagg
     4501 atgaataatt tgtataaatt agttccaaca agacgtttta tcaagcaatt gaaaaaattg
     4561 gaccgttata cgcagaagct aattacaaac tatttacaaa ccaatgtttt ggaagaccca
     4621 agacgacacg gaaaggcttt ggttggtaat cgcgttggtc aatggcgcta tagaattggt
     4681 aattatcgag ttatcgtaca aattgtagat gatgaattag tcgttgctac tctagaagtt
     4741 ggtcatcgga gagatattta ttgaattact tttttcaagt aaattttcat tctttaaaat
     4801 atgatataat agggcttaca atataagaga ggttgctgat gacaatagat attagtgaag
     4861 aaagtttggc taaagaatcg gctgacttat taaaaattct tttaaaagat cgaacgacaa
     4921 agaaatcaat tgtttgggct acgcattctt atgaattgtt aggaaaggga tttgctccaa
     4981 gtgatcgcat caatccaagt aaggttactg gaaactttgc caacttgatt cagcccagat
     5041 ctgaaaaatc caaatatgaa caaaaagatc ggacaaaaat aagggcagaa gtgtttacac
     5101 caacgtggtt ggtcgcaaag caaaatggat atgtagaatc gaaattaggc tcgttaagtc
     5161 ttgaggagta tgtggactta cgatggttag aggtaacttg tggtgaagca ccttatatgg
     5221 taacgagata tgataccgta actggtgagg aaattccatt atctgagcga gttggttttg
     5281 ttgatagaaa actgcaacgg attagtcgtg aggtttcaga tgaagtcaca ttttataaac
     5341 ttgtaaaaga tgcttatcat gcttcgtatg gttatgagta tcaaggagat tctctccttt
     5401 tggcgcgtga gaatcttttg gcaacttttg aggactatta tctagctaag actggaaatc
     5461 aaccaacatt agagcaaaag aaagaaattg cgactattat ctcttacaat gtctttcaga
     5521 tggatggatt gaaaaaaagt agtccttatt cagctaaaca aaagcaatct caacaattga
     5581 gtttgtttgc ggatgagcta gaagtccaag aagtagaaga atctaaaacc catatcaaag
     5641 actggaaaaa gaatagaatg ataggttttg agcgcctatc tagcgaggaa agtgaaatga
     5701 aatttgatgt cgtgatagga aatcctccgt atcaggaaac aggagaagcc agagatgagc
     5761 cgatatatca ctattttatt gatgaagctt ataaaatcgc agataaatct atactaatca
     5821 ccccagcaag gtttttattt aaagctggtc aaactcctaa aaattggatg gaaaagatgc
     5881 ttgaagacaa gcatttaaaa gttcaattct atgaacttaa aagcggtaaa gtatttacag
     5941 gaacggatat taagggtgga gtagcaataa cttatcgtga tgttgataaa gatttgggac
     6001 caatcggagt atttactatt tttgaaactt tgaatggtat tgtaaaaaaa atcgaaaaac
     6061 atccagaatt tgaaactttt tctgatttag t
//
LOCUS       DAVKLW010000089         5723 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKLW010000089 DAVKLW010000000
VERSION     DAVKLW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.089"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..237)
                     /locus_tag="VW324_001974"
     CDS             complement(<1..237)
                     /locus_tag="VW324_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727532.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYQVPILKNEYTVKLLLLME
                     CRIEDQEVIEYLVSTWKYRDLVLHSVKMYEIEKSE"
     gene            complement(492..1448)
                     /locus_tag="VW324_001975"
     CDS             complement(492..1448)
                     /locus_tag="VW324_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9727533.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLEGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     SWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1452..1784)
                     /locus_tag="VW324_001976"
     CDS             complement(1452..1784)
                     /locus_tag="VW324_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU9727534.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(1788..2087)
                     /locus_tag="VW324_001977"
     CDS             complement(1788..2087)
                     /locus_tag="VW324_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727535.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(2097..2447)
                     /locus_tag="VW324_001978"
     CDS             complement(2097..2447)
                     /locus_tag="VW324_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727536.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTGEPKE"
     gene            complement(2450..2653)
                     /locus_tag="VW324_001979"
     CDS             complement(2450..2653)
                     /locus_tag="VW324_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727537.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITNNILSIQLDEQREGNQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(2634..4016)
                     /locus_tag="VW324_001980"
                     /pseudo
     CDS             complement(2634..4016)
                     /locus_tag="VW324_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
BASE COUNT         1448 a         1338 c         1031 g         1906 t
ORIGIN      
        1 ctcactcttt tctatctcat acatctttac cgaatgcaac acgagatcac gatacttcca
       61 agtgctgacc agatactcaa taacctcttg gtcctctatc ctgcattcca taagtaataa
      121 tagtttgacc gtgtattcat tcttcaaaat cggaacctga taagtcacat ctacccaatg
      181 ctcaaaacct aagtctgtct gctctacatt tgctagttca atatttaaaa tcttcatttt
      241 tattcctcct acttatctat tcgtaaaaga agataaaaag ttatgaaaaa atcattactt
      301 ttttaattca gacagtactt tcagagcaat tttgttaaaa caaaattggc aaccaagcga
      361 tttaatacta tttgttgacg ccaacaagtc aattttcaga caaacaaaaa aaccgcaagc
      421 ctgagcctgc ggtgaaagaa caatttagaa agtttccttt ctatttattt aactgtaatc
      481 aagccatctg gttatttaac tgtaatcaag ccatctggct ctactgtgaa ctctggcttg
      541 tctgccagtg ttccgtctgg tttgaggtag taccagcctg ttccgtctgc ggactggacg
      601 aaggcgtttg ataccatagc gccttcttta gcgtctaagt agtaccatgt gtccttgtac
      661 ttgacccagc ctgtcttcat ggcaccttct tcgttgaaat agtaccactt ctcagcaatt
      721 ttcttccagc ctgtagccat ttcgcctgag ttgtcaaacc agtaccagtt gccgtctgtg
      781 tacttcttcc agctgtctga aagcatatag cctgagccat cgaaataata ccaggtaccg
      841 ttgattttct caaacttatc ttttggataa gagccgtctg agtgtacgta ccagtagcca
      901 gtgccatttt tctgccagcc tgttgcagcg ctcaagccgt tttcgatgtc ttgcttaaac
      961 tgctcacggc taatgcccca acttgcaaga tatggatatg gatccacatg gtctgagtgg
     1021 ttgtttggtt ggttattggt acagtattca tgcgtcttga taccttccaa gtcgtctgta
     1081 tcaagagtct tcggcaaacc tgcttcatct gctagattgc gtaagagttc gatataaagg
     1141 cggtagtccg tcatgaactc ttctttagtt gaatggcttt caatcagttc aaccgctgcg
     1201 taactctcag tattccaacc gcccccaaca tcccaacttc cgttgttcac aggacctact
     1261 tgcatgacac ggccatttcc gacaacatgt gaaaagaacc ctagttcagg gtcctttcta
     1321 tagtggtaat cagcctcatt ttgagctgtt gagttacggt tgcctgttga atgggcgtgt
     1381 acttgtcgat aaggctgcac cccaacctgt ggcaagcctg tacgtagtct gtttctatcg
     1441 atatccattc cctatcgtcc tttccatgcg tcattcatct gcttcactgc tgactctacg
     1501 aaggtgtcta agtctttgtc agtcatgcta atattgtatt ttgtaagctc agcacggact
     1561 ttagcgcgag cctgtgccag tttttcatct cccttgtagc cagtttcagc agctacttgc
     1621 tcaacggcat ttactgcatt tttggccaag atttcaacga ttttgatggt cttttctcca
     1681 cctttttgaa ccaggtagtc cttgactgcc ttgactgcga taccagccaa aataacaagg
     1741 atgctgattg ctccattagt aatgatttca gtaatttgtt gcatttgtta ttctcctttt
     1801 tcgatttctt ccatgcggtc gttcatgcga accatttctt tttgaatgtc tccgaccgcg
     1861 tgagtgattg tggttaattc tgtagtggtc ttttctaggt gagtcatcaa acgctcttct
     1921 cgtctattag agtcggcctt tgattgctcg tgcaaatcca taatcttctt ctctcgcttg
     1981 tccgaagtct tgataagata tcgaatgata ataaagaaaa gcaagataaa caaaatcgcc
     2041 caagctacct gactttgagc gattttttca gcttcttcaa ttggcataca cacctcctat
     2101 tctttaggtt ctcccgttgg atccgtccag tcaggattgc cctctgcatc aaatttcatg
     2161 atatagaatt catgattcaa cagaacggcg acgttgattg ttgcgattgt accaccccac
     2221 tggttgaacg cccaaacggt ttcaacatcc ttgagttggc gacggccatt tacgatcaca
     2281 ggacgttttt gaacatcacg atacatatag aagtcatcgc ttacattctt gcaacgaatg
     2341 aactctccat tttctttcat gtagcgcaaa gcactcgcaa gatcaaatgg ttctgtgatt
     2401 tttgtaaggt ctagtaagtt atctgtgttt tgatttgttt ctgccatgtc tattctcctt
     2461 tgtctgctgg tttagtttgt tcatcaagca gagcttccag ctcatccact cgtgcttgaa
     2521 gtctttgatt cccttccctt tgctcatcca actgaatact caagatatta tttgtaatca
     2581 tcgaatttgt tgaagttgtt gacatttcac taattgtcat ttgtaaggct tggttaagct
     2641 gttctgtgtt cattttctaa gttctccaat ctgtgtgttc gttttctatt ttcaagagca
     2701 agctcctgaa ttgctttaaa gtgcgatatt ggtcaacatg ttgttattca tgctattgtt
     2761 ttctccattt tttctatttt ttgatttaat tcttgaatag ccttgattaa gtaaggaact
     2821 aaagcggtat agtcaatatg cagatagcca tctggattct caggatctcg tgagacaatt
     2881 cttggaacga tggtttcagc ctcttgagct attagaccaa tctcctcatg tttcttattt
     2941 tcgatgaaat caaatgcaac cattcttaat ctgttgattt tgtccaaggc tttcacagct
     3001 gtatctgtga tgttctcttt taagcgtctg tctgattttt gttccatcca atacttcacg
     3061 ctaccgctac cgacctgatt ccaccaaaca accgcattcc ttccgccttt gggattccaa
     3121 ccatcaccaa gcacatcttt acttccaagt tcgataccat ttgaaaacac aggagaacga
     3181 gaaaaagtag tattcccata gaagtttgct ctcgatgaat tcgaaaaatc cacttgatca
     3241 taaaaaccga cttcattcct acagtacatt ttcccatcag tattgacgtt ccatgcttta
     3301 ggtccggcat agttccaatt atttccccag ttcgcccaga aggctgtccg gactccatgc
     3361 ccggcaccat tccccatacc aacagagaac tgattgacac ctgaaatcca gcgaccgcca
     3421 ccctggtcaa attgaccaag tgtgaatcca ccaattcggc cttgataggc ttctaggaag
     3481 gttgagctag aaatgacgga ctcaacctta atagagaaga tacgtttaga tgtcagtcgg
     3541 tcaataaaag catcttttgc agttaatttt ctaataagcg catcgtcaac tttcaacttc
     3601 tcagcagtta ccgcttcagc ttctaatatc gtagtcgtga ccgaaccagc ttcaaaattg
     3661 gccgttttga gcttatcaac catggcagac ttgatgactg ctctgtcaat cagggtctct
     3721 ccagtgatgt gggtcaattt cccaactaag cggttatgtc cattggcgcc aagattgatt
     3781 ccagagatga tatctccagc cgagttgatg ttttcaacta cccatgagcc agctagttga
     3841 gtcatttttg tttgcgttgc ttcaagcttc ttattcgcat ctgcgactgc atcttctgga
     3901 tgtggttgcc atgttctaga tttataacct ttgtacaagt caacttctgt aatatacaaa
     3961 tcagctgttc ctgatgatga gccattgtta tcaaaacgaa tgtaagcatt atccatttct
     4021 ccggaattaa aagttactga gacatcttcg catctagagg tagatagttt cttgctgcta
     4081 acaactttct taacgattgt gaatccatcg ctctcgcctg ctcttcgtcc caaaatataa
     4141 acatcatagc ttgctagagc actgttgtta aatcctctaa aattcagtac atagtcagta
     4201 tttcgttcaa gattaaaacg gtgactatac aaaaagtttt cgtttttagt tgcattactt
     4261 aaacgcataa ggtctttctg cccgttgtga taaaagctat gcttaaccaa tcttcctaaa
     4321 ttttgagttg agccccattc attcgtacca tttttaaaat cactattctt aatgaggtta
     4381 gggccgctta cactatattt cccaacctca acctgaaaca gttgattggt cagagccatg
     4441 cgagcaacct tatccgcaat tccattttca gtattgccca aaatccgctc gtaaagttta
     4501 ctggtttcct taacacgctg gaagtcagta gtctctactt ttcgcactag ttgattggtc
     4561 acattcgcaa attgactatc agcattcgct ttgtttgcag aaacctgatc agatattcta
     4621 cccatttgtc gttcagcatt atccttgttt gtagcgacct gagtctttaa atttgaaatc
     4681 tgattatctg cgccttgttt attactgttt atccgatttg aaagatttga aatctgagta
     4741 gtggttcctt gctcactgct tgtaagtcta tttgaaaggc cactgatttg accgcccaca
     4801 tcttgcttat aagtagttat ctgacttgaa atatccgtga acttaccatc tacagattga
     4861 cgatagctag cgatttgact agcgatgtct ttattcgcac tagttttaac agcttcaatc
     4921 ctttgattga tacccttaac atcttcttga taagtagcct taccaacgaa atcacgattg
     4981 accagctcac ggactgctgt cgcttgtctc gcgctctcct cacgagtata gcgctgtagg
     5041 gcttcctgtc gctgaccgtc tttatttaca tattcctgaa tagctgataa gtcggttcgc
     5101 aagccctgag ctgtccgctc aaaggtagcc ttagcttcag taatgagacc atcagcgtcc
     5161 tcaggcgcag gactccagtc cgtcgccaca ctaccgattt caaccttgat tcctgttacc
     5221 caagctgtac cgcttgtagc accttcaaga ttgaatcgca atgatgtctt caattgatca
     5281 aaatttgttt tttcagagta gtcataagtg aatgtaatat atttccaatc tgccgaacct
     5341 ttatacatac caagcgtagc ataatctgga ccactctgta ctccggtctc actatttttt
     5401 ctaaaaagat aatgtttgaa gcaattaaat acattccaaa aatttcgacc ttggactaca
     5461 ttttcgtact tgatccaagc gctaaaagta actttttggt acaaccttga gctgaaatct
     5521 ggttcaaggt tgaacgttaa agtagagttg ttctctagcc tatagcattc tttttgacct
     5581 gtgacgtggt tttcaggtaa tttttcaatt actgccccaa ctgtccggga ttttatccat
     5641 agattccgcc caccgacttt catcttcgaa aattcttcac gaattttccc agcttcagcc
     5701 acgacaagag tcttatctgc ctt
//
LOCUS       DAVKLW010000090         4953 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKLW010000090 DAVKLW010000000
VERSION     DAVKLW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.090"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..274)
                     /locus_tag="VW324_001981"
     CDS             complement(<1..274)
                     /locus_tag="VW324_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727538.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLIS"
     gene            complement(271..624)
                     /locus_tag="VW324_001982"
     CDS             complement(271..624)
                     /locus_tag="VW324_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9727539.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            1383..1703
                     /locus_tag="VW324_001983"
     CDS             1383..1703
                     /locus_tag="VW324_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9727540.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(1755..2342)
                     /locus_tag="VW324_001984"
     CDS             complement(1755..2342)
                     /locus_tag="VW324_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9727541.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            2584..3255
                     /gene="sdaAB"
                     /locus_tag="VW324_001985"
     CDS             2584..3255
                     /gene="sdaAB"
                     /locus_tag="VW324_001985"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9727542.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            3264..4136
                     /gene="sdaAA"
                     /locus_tag="VW324_001986"
     CDS             3264..4136
                     /gene="sdaAA"
                     /locus_tag="VW324_001986"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9727543.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            4158..4790
                     /locus_tag="VW324_001987"
     CDS             4158..4790
                     /locus_tag="VW324_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9727544.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
BASE COUNT         1521 a         1045 c          890 g         1497 t
ORIGIN      
        1 cacttattaa tacggtataa gtcaaaaaac tttctgggaa aaacttattg attaaaatgg
       61 ggaaactcat taacagaatc gaatttatgg ctagacagat agatactatt ttatttttca
      121 cagaatatag aggtagtgat actattgagt agataaaagc taaaaaaata agcaaaaaat
      181 actccgtgtc atttataatc gccctgaatg ataaaaatat atacatccaa catacgataa
      241 tgtgtatttt tcgaatctta tctacattac tcatcttact gtcgcagata tgaatccatc
      301 ccaaggataa gggttcccgc taatatcaca acctctatta agagcaccat atccaataca
      361 gtaagctatt cttcctgccg ccccagccaa tacacctgct gctgcaccaa taagccaacc
      421 tccaaggcct ccaactttaa tatagtttat tagttttttg gttaaaactg cagctccagc
      481 taatgctata aaagttcctg gactcatagc taaagctgcg caaattcctt gacaagcacg
      541 cttgcttaaa taagctcccc catcaaggta catcatctct tcttcatcaa tcacaacata
      601 attatttggt aatacaagtt ccatttgttt tctccttttt tattaagcat ggttaggatg
      661 gtttcctaac aagtgatcgc aggctttgtt tagttaccta aattagactt cctttcttta
      721 tcttatttta aatatttaaa accagttact gcttgaagct taacagttta cttaattcaa
      781 ataacagcta atcactgatt ccctgatttt atcaaatata ctaataacaa caattgtgat
      841 acgatcatca ttataagaac aacatttaac gaatcaaagt ttttccgaga taataataaa
      901 gcaacagcaa gcagtattat tactaaagat tgaataacta ctattactct taacatttta
      961 tcctccacta acaactcaag ctcccttaca ttatatcgac cagccccatg gaaagattat
     1021 agctagacta atcaatgata taatacatac tagaatacaa aatatttttt tattcatact
     1081 ttttctccta tctttatttt ctacctacat agtacctctt attaagctca tttcaaaatt
     1141 acgttaactt ttttcacgaa aaggagaaaa ttatgcgtta tgattatgga tagcgctttc
     1201 aaatttttaa actcttatcc catcctttta tctatataat aagtgaaaat ataataactg
     1261 tcaagtaatc gaagtgaatt ttataaaaaa aattacaagc caaatttgca aagtttacaa
     1321 taagccgcta gggcaatcgt ctatcagaat gtccgtttat ttgtcaataa tccgagaaaa
     1381 tcttgcaacg cttagaagtc tataaaaact atcaacattt atatgacttg cgaatagcaa
     1441 tcctgctcaa cctttccaca ctatatctat acaatcaaga taaaaacatg tgtaagcaaa
     1501 tctgctacac tttactggag gacgccaaga ataagaaaag ctacgatagg ctggctatct
     1561 gctatgtccg cattgggatt tgtacagacg attctaaact tatccaaaaa ggtttctcac
     1621 tcctagagct gaccgaggaa acttctatgc tgtctcatct caaaaaagaa gtagagatct
     1681 attaccaagc gaaggaaaga taaaaaagcc gagggatttc ctcgactctt tacatattat
     1741 ttgttcgtta gttcttaata ccagccattg ttaagccaga agtttttagc agcagtccat
     1801 gaaccgtaac gtcctgcaac gtaggcatct gctacacgtt cttggttttc agctgagtag
     1861 tcaccgttca ggtatgaatc tgttaattgg taacgtccga tataacgtcc atttgtagct
     1921 gtatagctac cacctgattc tttttgagcg atccattctt tggcttctgc ttcagatccg
     1981 cttacagttg aaacaactgt ttcacttact actggagttt ctgctactgg agctgaaaca
     2041 gtttcatctt gagcggctgg tgccgcataa gtagctggcg ctggtgttgc aacaggcgct
     2101 acagggccat cgataaccaa ctcttgatca acataaatca aatgaatgtt atcaatgtgg
     2161 ttgttttctg ccaatttttc aactgttgtg ttgtgagttt cagcgatttc tgaaagtgta
     2221 tcaccttctt taacagtgta agttgatgat tcttgagcag atacaaatga tggagcaaat
     2281 actgcaaaca aggcagctac tcctgcaaga gttgctttaa tctttttagt tattgatttc
     2341 atattcgaaa attctccttc tttctatagt tctatcatac cctttaaata ttaccgtttc
     2401 ttgacacttt tatgtagaaa tattacaaaa ttatttttta tttccctaaa taaggtgttt
     2461 tttgtcataa aagatactat ttttatgcta tttgaggtat tagaaaggtt ttcatccaca
     2521 attaaagcca agcccctcat tctttgatat aatagagata agaattttta taaggggaaa
     2581 ctgatgaaat cacttcgttt tcaatctgtc tttgatatca tcggaccagt tatgattggc
     2641 ccatctagta gccataccgc tggtgctgtt cgtattggga agattgtctc ttccattttt
     2701 gatgatactc cgacagaagt tgaattccaa ctttttaact catttgccaa gacctatcgt
     2761 ggtcacggaa cagacctagc ccttgttgca ggtattttag gaatggatac agatgatcct
     2821 gaaattccaa atagtctgga aattgcccac aagcgtggta tcaagattgt ctggaccatt
     2881 cagaaagaca gcaatgcccc tcaccctaac accactaaaa ttactgttaa aaatgcccac
     2941 aagaccatca gcgtgactgg tatttctatc ggtggaggaa atattcaagt aaccgaactc
     3001 aatggttttg ccgtctctct caatatgaat acaccgacta tcatcatcgt tcatcaagat
     3061 attccaggta tgattgccct tgttacagag gcgctttccc gctatggtat caatatcgcc
     3121 cagatgaatg tcactcgtga aaaagctggt gaaaaagcca ttatgattat cgaagttgac
     3181 agtcgcaact gtgatgaggc tatcgaagaa attcgaaaaa tccctcatct ccacaatgtc
     3241 aatttcttta aataggagga agcatgtttt attctatcaa agaattggtc gagcaagcag
     3301 atctggactt tcaaggaaat gtcgcagaac tcatgattac aacagagttt gaattgaccg
     3361 gtcgcgaacg tgaagaagtc ttccttctca tggaacgcaa tctggaagtt atgaaagcct
     3421 ctgtccaact tggcctcaat gaaaataaat ctcgtagtgg cctgacaggt ggagatgctg
     3481 ccaaattgga tcactacatc gaaaacggaa aaactctgtc agattatacg attctctcgg
     3541 ctgcccgaaa tgccatcgca gtcaatgaac acaatgccaa aatgggcttg gtctgtgcca
     3601 ctccaaccgc tggaagtgct ggctgtctgc catccgttct cactgctgct attgaaaaat
     3661 tagacctcag ccacgagcaa caattggatt tcctctttgc tgctggtgcc tttggactag
     3721 tcatcgcaaa caatgcctcc atctcaggtg ctgagggtgg gtgtcaagct gaagttggtt
     3781 cagcctctgc tatgagtgct gccgccttga ctctggctgc aggtggaaca ccttatcagg
     3841 ccagtcaagc tattgccttt gtcattaaaa atatgctagg cctcatctgt gaccctgttg
     3901 caggtttggt cgaagttccc tgtgtcaaac gtaatgccat gggagctagc tttgctttca
     3961 tcgcagcaga catggccttg gcaggtatcg aatctaaaat ccctgtggat gaagtgatcg
     4021 atgccatgta ccaagtagga gcaagcatgc caactgcctt tcgtgaaaca gctgaaggtg
     4081 gactcgctac cacccctact ggtcgtcgcc tccaaaaaga aattttcgga gaataagctt
     4141 atcaaatagg agaaaccatg acctctatca cagcgatttt tttcgatttg gatggaaccc
     4201 tcgttgatag ttctatcggg attcacaatg cctttaccta tacctttaaa gagctggggg
     4261 tgcctagccc tgatgccaaa actattcgtg gttttatggg accacctctc gaaagtagtt
     4321 ttgcgacctg cctgtccaaa gaccaaattt ctgaagccgt gcagatatat cgttcttact
     4381 ataaggcaaa aggcatctat gaagctcaac tctttcctca gattatagac ttgcttgagg
     4441 agttatcgag cagttatcca ctctacatca ccacgacaaa ggatacatct actgctcaag
     4501 acatggccaa aaacttggaa atccatcatt tctttgatgg catttatggt tctagccctg
     4561 aagcacccca taaggcagat gtcattcacc aagccttgca gacacatcaa ctagcaccag
     4621 aacaagccat catcatcgga gataccaagt ttgatatgct gggagctcga gaaacaggta
     4681 ttcagaaatt ggccatcact tggggatttg gagagcaagc agatctacta aactatcaac
     4741 ctgattatat cgctcacaaa cccttagaag ttttggcgta ttttcaatag catagactgg
     4801 gtaacaattc catttcagaa gaaaatgaag taatactcaa tgaaaatcaa agagcaaact
     4861 aggaaactag ctgcgggctg ctcaaaacac tgttttgagg ttgcagatag aactgacgaa
     4921 gtcagctcaa aacactgttt taaggttgtg gat
//
LOCUS       DAVKLW010000091         4947 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKLW010000091 DAVKLW010000000
VERSION     DAVKLW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.091"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(417..1145)
                     /locus_tag="VW324_001988"
     CDS             complement(417..1145)
                     /locus_tag="VW324_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9727545.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1303..2712
                     /locus_tag="VW324_001989"
     CDS             1303..2712
                     /locus_tag="VW324_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9727546.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2730..4025
                     /locus_tag="VW324_001990"
     CDS             2730..4025
                     /locus_tag="VW324_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9727547.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4030..4338
                     /locus_tag="VW324_001991"
     CDS             4030..4338
                     /locus_tag="VW324_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9727548.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4335..4643
                     /locus_tag="VW324_001992"
     CDS             4335..4643
                     /locus_tag="VW324_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9727549.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1676 a          826 c          909 g         1536 t
ORIGIN      
        1 aatgattatg atttagaatt tatttaatat attaaaagaa gatatgaaca aataaattgg
       61 gagattgaaa ttcatttcta gcaatttgtt agaagtcaca gtctactatt ctaaccacag
      121 tatactatat gtgttacaag aaaagactca aaaacttctc gtcagtgtaa gaatgtttga
      181 aacactattt aaaactaaat aactataaga agtagatcag gtgaacaatt tcagtaccta
      241 ctctataagc tatacgaaga tatttttcaa catgatggat acaacgatca ctcttttaaa
      301 gccatcttat ttagaaaaga gaaaccattt aagcatagat gggaccttct tgccctgttt
      361 taaatttttc ttaaactaga gtctgtaaaa gattggcttc attttcacat tttctcctag
      421 gaggaatgtc gtaatgcaaa agaattaaat tcaaataaat catagcgatg acgactaatc
      481 gagtactcaa acggagttcc gttatccaaa taagccactt gttctacttc aaataccggt
      541 tccgttggaa ggagctgtaa gtaatgttgc tcattttctg aactagaaga agctcgcaag
      601 atttttgtag cactggcaat atgcaatcct aactttcctt caatgtaatt gtaaatcgat
      661 ttttgtaaaa tatcttcagt aatacctgga ataacatcgg tactcatata agtttgttcc
      721 agaacataag gtttaccgtc aataatacgc aggcggtaaa tattataaac tggactctgc
      781 aaagcgacct gtagtttttc tgctaaaaat tcatttgcaa attctaattt gaagtgaata
      841 atcctcgagt caattgtaga atgagcatct tcagatattt ttgtcaatcc ccggatatct
      901 ctttctggaa cgattaattt tctttttgag ctgccacgag agagaacaaa ggttccctgc
      961 ccacgttttc taaaaattaa gccctcagaa actaacaagt ctaaagcttt tttgatcgtc
     1021 attcgactac aggcaaactg tttagctaat gtcatttcat caggcaattg ttcgttcatg
     1081 gtatatttat tacaaagaat cttttcacga atctcattgt aaataaataa atattttgta
     1141 ctcatttaat ctggttctct ctataatatt ctaatagtag tataacaaag ttcaagaaat
     1201 gaggcaaaca caagatatgt atcaacaatt tttaaaaatg tctagacaaa ttttaaaatt
     1261 atgctatact ttatgtatag aataatagaa aggaggttga gcatgaaaaa aaagttagta
     1321 tttcctaatc tgttttggtg gggagctgct tctagcggac ctcagacaga aggtcaatat
     1381 ggaaaagtac atgaaaatgt gatggactac tggttcaaaa cgcatccaga agattttttc
     1441 gataatgtcg gacctcttgt agccagtaac ttttttcata cttacaccga agatttccac
     1501 ttgatgaagg aaattggagt taattctttc cgcacttcca tccaatggag tcgactcatc
     1561 aagaatttag agacaggtga gcctgatcca aaaggtattg ctttctacaa tgccatcatt
     1621 gaagaagcta aaaagaacca gatggatctt gtgatgaatt tacatcattt tgatttacca
     1681 gtggaacttc ttcaaaaata cggtggttgg gaaagcaaac atgtagtgga gttattcgtg
     1741 aagtttgcca agactgcttt cacatgcttt ggagataagg ttcattactg gacaactttc
     1801 aatgagccaa tggtcattcc agaagcagga tacttatatg ctttccatta tccaaatcta
     1861 aaaggaaagg gaaaagaggc cgtacaagtc atctataatc taaaccttgc tagtgcaaaa
     1921 gtgattcaac tatatcgctc attaggactt gatggaaaga ttgggattat tttaaacttg
     1981 acacctgctt atccaagaag taattctcca gaagacttag aagcaagtcg atttacagat
     2041 gacttcttta acaaagtctt cttgaatcca gctgttaaag gaactttccc agaaagattg
     2101 gtaaaacagc tagagagaga tggcgtgtta tggagtcata ccgaaaaaga gcttcaactg
     2161 atgaaatcaa atacggttga ttttcttgga gtaaactact accatccaaa acgtgttcaa
     2221 gcacaagcaa atcctgagga atatcagacg ccctggatgc cagaccaata cttcaaagag
     2281 tatgaatggc cggagcgtcg catgaatcca tatcgtggtt gggaaatttt tccgaaagcc
     2341 atttatgata ttgctatgat tgtgaaggaa gaatatggta atatcccatg gtttatcagt
     2401 gaaaacggaa tgggtgttga aaacgaagca cggtttatcg gtgaaaatgg agttatcgat
     2461 gacgtgtatc gtattgaatt ttatgaagaa catttaagat ggctacataa agccattgaa
     2521 gagggaagtc actgttttgg ataccacgct tggaccgcat ttgattgctg gtcttggaat
     2581 aatgcatata agaatcgtta cggatttatc tccgttgatt tagaaacgca aaagagaacc
     2641 atcaagagat caggaagatg gtatcgcaaa gtaagtgaca ataacggttt tgaagtagaa
     2701 attgaggagt aaagagagga gatattcaga tgaaaaactc aaaatttata gatcaatttg
     2761 ccacctttgc tggtaaacta gggaaccaaa ttcatttaaa aaccctaaga gatgcattcg
     2821 taacagtaat gccattatat attttggcag gtttaatcgt tcttttgaac aacacggtat
     2881 ttaagtggat tttccaaggg gatacattaa caagattcca atattgggga ataacaattg
     2941 caaacggtac tttaagtatt tcaggtatga ttattgctgt aatggttggt tatttcttag
     3001 ctaaaaacag agatttcgaa aacccgttag cagcatcaat gctatcatta gtttctttaa
     3061 ttgtgatgat gccaaataca gtttctgtag ttcctgacgg agcaaaagat gcggtaaaca
     3121 tttcaggtgt tctttcattc aacaacacag gtacaggcgc aatgttcgcc ggggttatcg
     3181 tagcgattat tgcaacagaa ttattcattg aattatcaaa cgttaaagct ttacaaatga
     3241 accttggtga aaatattcca ccagctgtta gtagatcatt tagcgtatta cttccagtca
     3301 tgaccgtcat ctccttattt ggggttgttt cagcattatt attcaatata actggaatga
     3361 acttaatctc aatcattaca atctttattc aagaaccaat tcgtcatatt ggtacaagct
     3421 taatcggggt cattattatt tactctttag gaaatatgtt atggctattt ggtattcacc
     3481 aagcagttat ttacagtgcc atcctagaac cattactatt aattaacatt actgaaaaca
     3541 tcactgcagc aaataatgga caagccattc cacacatcat caacctatca caaatacaaa
     3601 cattcgcttt aatgggtggt agtggatcta cattatgttt attaatagca acattcttag
     3661 tgagtcgcaa tgctgtctct aaaaacgtgg ctaaattatc ttttggacct ggtatcttca
     3721 atatcaatga accagtatta ttcggttacc caatcgttta taacatttca ttagctattc
     3781 catttatcac agttccagtc cttggtattt taatcagcta cttagcaaca gttacaggat
     3841 tcatgagtcc tgcatttata caagttcctt ggactacacc agtattctta aatgcatggt
     3901 tagcaacagc aggggacgtg agagcagttc tagttcaatt catcatcttt gcacttggag
     3961 ttcttctata cattccattt atcaaagtta atgacaaagt tgttgaacaa gaaatggaag
     4021 gttaagatta tgaaacaaat tttattagta tgtaatgcgg gaatgtcgac aagtatgctt
     4081 gttaaaaaaa tgcaacaaag tgcgactgaa cgtgggattg aaatttctat ccaagcaaaa
     4141 tcaatgacag aagctaaaaa gaatatccat gaagcagatg tcattctaat tgggccacaa
     4201 attcgttatg agttacttgc tgtgaaagaa attgcaggaa atatccctgt agataccatt
     4261 gatatgcggg attacggaat gatgaatgga gcaaaagttc tggaacaagc acttgcatgg
     4321 ataggagaaa taagatgaca gaagaaatgg aatatatctg ttttcagctc attgccaata
     4381 gtggagcagc caaatcatcc tttattgaag ccatccaact agctaaggca ggaaatttaa
     4441 aagaagcgaa aattaaagtt gaagaagcgg aagactcctt agtagaagcg cacaagattc
     4501 actctaatct catacaaaaa gaagctactg gagaaaaaat aggattctcc ttgctattta
     4561 tgcatgcaga agaccaaatg gcttcaacag agattattca gttgctatct aaagaattca
     4621 tcgatctcta tcaaaataaa taggaacact gtcattccca tctgtcttta ttaatgacgg
     4681 ctacattgta ggaacctcca aaagttggac aaaatgattg aaaggattgg atcctgcatt
     4741 acacaagatc caatcctttt cttttactct agtttttata atgtgttgaa tttagaatag
     4801 tccactgcag tttctaaaaa attgctggaa atagatgtta agctccaatt tatttgttta
     4861 tatcttattt cagtttacta tactagctta ttattctagt aaaaaattga atgagccact
     4921 atttatttaa tatgtatgat gagggaa
//
LOCUS       DAVKLW010000092         4892 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKLW010000092 DAVKLW010000000
VERSION     DAVKLW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.092"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            89..685
                     /locus_tag="VW324_001993"
                     /pseudo
     CDS             89..685
                     /locus_tag="VW324_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            761..1210
                     /locus_tag="VW324_001994"
     CDS             761..1210
                     /locus_tag="VW324_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9727550.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1223..2298
                     /locus_tag="VW324_001995"
                     /pseudo
     CDS             1223..2298
                     /locus_tag="VW324_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2288..3933
                     /locus_tag="VW324_001996"
                     /pseudo
     CDS             2288..3933
                     /locus_tag="VW324_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4036..4728)
                     /locus_tag="VW324_001997"
     CDS             complement(4036..4728)
                     /locus_tag="VW324_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9727551.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1545 a         1036 c          843 g         1468 t
ORIGIN      
        1 aaagtgtact gttatagatt caatatacta tagaatagaa ttgtaggttt taaaataaac
       61 ttaaataaca caacggagat atcccatcat gaaaaagaaa tggatgtatt atgctgcttg
      121 ttcttctaat gaatctgccg atgacagttc atctgataaa ggagacggtg gttcgctagt
      181 cgtttattga ccaaactcag aggacttaat tggagcaact attcctgcct ttgaagaaaa
      241 atatggtatc aaagtagaac tgattcaagc tggtactgga gaacttttca aaaaactaga
      301 gtcagaaaaa gaagttcctg tagctgatgt tatctttggt ggttcttata cacaatatgc
      361 tacccacgga gaactctttg aaaactatac ttcaaaagaa aatgataatg ttatcaaaga
      421 atatcaaaac acaactggct actctactcc ttatacacta gatggtagtg ttttaatcgt
      481 caaccctgat ttaactaaag gcatgaacat cgaaggatat aacgatcttc tcaaacctga
      541 actaaaagga aaaatcgcaa ctgccgaccc agcaaactct tctagcgcct ttgctcaatt
      601 aacaaatatg ctacaagctc aaggtggtta caaagatgat aaggcttggt cttatgtaaa
      661 agatcttttc acacttattg atggtatagt aaaatgaaat aagaacagga caaatcaatc
      721 aggacagtca aatcgatttc taacaatatt ttagaagtag atgtgtacta ttctagtttc
      781 aatatactat aaaatcggtt taagttcatc tggtgtctat aaagcagtcg ctgatggaga
      841 aatgactgtt ggtctctctt atgaagatcc agcagttaaa ctcttaaatg acggagctaa
      901 cattaaggta gtctatccaa aagaaggaac cgtcttccta cctgctagtg ctgctatcgt
      961 taaaaaatct aaaaatatgg aaaatgccaa gaaatttatc gattttatta tctctcaaga
     1021 agtacaagat acacttggta caaccactac taaccgtcct gttcgtaaaa atgctaaaac
     1081 aagcgaaaac atgaaaccaa ttgacaaaat caaaacactc actgaagatt atgattatgt
     1141 catcaagaat aaatcagata tcgttaagaa atacaacgaa gtctttacag atatccaatc
     1201 taaacagtaa aagaggttca ctatgagtga gatcaaaatt attaacgcca aaaaaatcta
     1261 ccacgatgtc cctgttattg agaatttgaa cattacaatt ccaaaattct tggagcttca
     1321 ggatgtggga aaacgaccct tcttcgtatg attgcaggtt tcaacagtat caaagatgga
     1381 gaattttact tcgatgatac aaaaatcaat aatatggaac ccagcaaacg caatatcggg
     1441 atggttttcc aaaactacgc tattttccca catttgactg tccgagacaa cgttgctttt
     1501 ggtcttatgc aaaagaaggt tccaaaagaa gaattgattc aacagaccaa caagtatctt
     1561 gaactcatac aaattgctca atatgcggat cgaaagcccg ataaactcag tggtggacaa
     1621 caacaacgtg tcaccttggc atgcgcctta gcggttaatc caagtgttct cgtcatggac
     1681 gagccactta gtaatctgga ggccaaactt cgcttggata tacgtcaagc catccgagaa
     1741 atccaacacg aagtgggaat tacaactgtt tatgtaaccc acgaccaaga agaagccatg
     1801 gctatttcag accaaattgc tgttatgaaa gatggggtga tccaacaaat cggccgacca
     1861 aaagaactct atcataaacc agctaatgag tttgtggcaa cctttatcgg acgcacaaat
     1921 attatccctg ccaatcttga aaaacggagc gacggcgctt atatcgtctt ttcagatggc
     1981 tatgctcttc gaatgctagc tcttgatcag gttgaggagc aagctattca tgtaagcatt
     2041 cgtcccgaag agtttatcaa agatgaatct ggagatattg aaggaactat tagcgatagc
     2101 gtctatcttg gactaaatac ggagtatttc attgagacag gttttgcctc aaaaattcaa
     2161 gttagtgaag aatcaacttt tgaagaagat ctacaaaaag gcaatcgtat tcgtctacga
     2221 atcaatacgc aaaaattaaa cagcttttct gcagatggtt cccaaaatct gataaaagga
     2281 gtcaaccatg gaatgtaaaa aactaaatat ttggacagct tcctctttct tcctctttct
     2341 tacctatcct atctttctcg tttatcctat cgttaccgtg ctcaagcaag cacttataca
     2401 tgaaggacaa ttctcactag ctaattttgt cactttcttt agtaaagcct actactctga
     2461 gacactagtc aacagtttca aggtttccat taccgctact atcacttcct tagttgtagg
     2521 aaccctatta gcttatctct tctctatgta tgacttcaag gggaagaaat ttctacaaat
     2581 attgattatc attgcttcca tgtcagctcc tttcgtagga gcctactcct gaattctctt
     2641 gctgggacga aatgaggtca tcactaaatt tttgacaaat gcccttcatc ttccagctat
     2701 cgatatttat ggattcaaag gaattgtact tgtctttaca ctgcaactat tcccactggt
     2761 atttctatat gttgctggaa caatgaacag tattgacaat tctctacttg aagcagctga
     2821 aagcatgggg tccttcggat ttaagcgtat cgtaacggtt gttttacctc tcctagttcc
     2881 aaccttacta gcagatcctt gcttgtattt atgagagcat tctcagactt tggaacgcct
     2941 atgttgattg gcgaaggata tcggactttc cctgtcctga tttataccca atttattagc
     3001 gaggttggag gaaattctgc ttttgcaatt atggcgatta tcattgcctt ggcaattttc
     3061 cttatccaaa aacacattgc aaaccgctac agtttcagca tgaatctgct ccatccaatt
     3121 gagcctaaaa aaactacaaa aggaaaaata gctgccattt atgcaacagt ctacggaatt
     3181 atctttatct ctgttttacc tcaaatctac ttaatttata cctctttcct aaaaacatca
     3241 ggtatggtat ctgttaaagg ttattctcca aacagttaca aggtagcttt caatcgtatg
     3301 ggatctgcta ttttcaatac cattcgtatc cctttgattg ccttagttct agttgttcta
     3361 tttgcgacat ttatctccta cctagccgtt agaaaacgga atttgtttac aaacttaatt
     3421 gacagcctca gtatggtacc ttatattgta ccaggaaccg ttctagggat tgccttcatt
     3481 tcttccttca atactggtct atttggaagt ggatttctta tgattacagg gactgctttc
     3541 atcttgatta tgtctctatc tgtcagaaga ttaccgtata ctattcgctc atctgttgct
     3601 agcttacaac aaatagcacc aagtattgaa gaagctgctg aaagcttagg aagtagtcgt
     3661 ctcaatacct ttgctaagat tacaactcca atgatgctat ctggtatcat ttctggagcc
     3721 atcttatctt gggtcacaat gatttcaaaa ctctctactt ctatcctcct ctacaatgtc
     3781 aaaacaagaa caatgactgt agctatttat acagaggttc tcagaggaaa ttacggtgta
     3841 gccgcagcct tgtcaactat cctgactgtt ctaacagtag gttccttgct cttgtttatg
     3901 aaaatctcta aaagcaatag cattacactt tagttttcca catcaaaaac agccgaaagg
     3961 agtaccctcg gctgtttttt cttatcttga tattcttatc aaatccctag ctcatggcaa
     4021 gcaagtctag actgattaaa tagagccttc ttccatcttc tcacgtccta gctctcggta
     4081 actacggtca ccgacaactt caaccctaaa ctgaccgtcc tcatattcaa gaatcgtcac
     4141 gctaccatta tctagaccat gcggatgcat gccattaatc agataaacaa tggttccaat
     4201 agtcattcca tggctgacaa caagggcgtt acctccacct tgatcttcca tttcttttgc
     4261 aatcatttca aagccttcct tgattcggcc actgagtttt tcccagcctt cagcccaacc
     4321 agctgtatcg acctctacca agccctcagc cagttcagca taagacaatt ggtgaacgtg
     4381 gtccacatta aagatacgag gaataatgcc catgaaaaga tcgccatcat aggctccatc
     4441 aaaactaccg aaacaccatt ctctgatacg cttgtccatg cgataaggga ttttcccctg
     4501 caagccaagt tcttcaagga taattcccat ggtctgaatg gtacgaccag aatcactcga
     4561 ataagcgcgc tcaaactgta gatcagattc tcgcaaaccg attcctaact cttgaatccc
     4621 tcgttcacct tcagcagtta agggagtatc gctccaacct tgcgcgcgac caatcgtgtt
     4681 aaacatggtc ttgccatgac gtaccaaata caatcgtact tttaccattt tccgtcctcc
     4741 gtttgcttct attatatcat gaatgataaa ataaaagcca cccatacagg cgacttttga
     4801 aggagattat tatgaaaaag tttaggagtt cattaaataa agatattaga tgaaaatcaa
     4861 attcaaacta attcagtgtt atctgtttca aa
//
LOCUS       DAVKLW010000093         4870 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKLW010000093 DAVKLW010000000
VERSION     DAVKLW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.093"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            81..608
                     /locus_tag="VW324_001998"
     CDS             81..608
                     /locus_tag="VW324_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727552.1"
                     /translation="MIKYKELKSYIDEWRYYSDENNPRLDLEYCKTKIVEKAKEFDLP
                     CQIDEEQIKLGGLFNKEIEECLVISHPNHQKDYVKFCFRLKNQGSVQLLTIDTLGESK
                     QLKKYYISEDNKRFREAIRESDLSLGQKLGAQLSNLTVSGLRTLGKNQSKIDAELQYC
                     EFLTEVLTQFRSNND"
     gene            618..2156
                     /locus_tag="VW324_001999"
     CDS             618..2156
                     /locus_tag="VW324_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU9727553.1"
                     /translation="MSKAIGIDLGTTYSAVSVLSETGQPQILLNQDGENLTPSVVFFQ
                     DFDGKDEPLVGIQAKNLAASRPEAVVQYVKRQMGNPNWKFDSPSDTVYTAEEISAIIL
                     KRLKEGAENALGDKVEDVVITVPAYFDDARRTATKHAGEIAGLNVLRVLNEPTAAALA
                     YGISAEKNETVLVYDLGGGTFDVTLMKIKDGEFDVIATDGDRNLGGFDFDNALSMIIA
                     EKMEEQGAEDIYTDEHFTALLREKSENTKRGLTTVEKTNVFLDYKGKSYKIPITRVEF
                     EEATKSLMNRTEELLDDVVEESGMSWDEIDQVLLIGGSTRMPMVQRKLEEKIGKKIVY
                     SINPDEAVAQGAAIQAALEIANKSESADVSETVRGLAEKLVVSDVTSQALGTLALSNG
                     VKRNTIIIPKNSKVPNKYSEYFSTVVDNQQNVLVEVTQGDDEDPQFVTVIGESTIKLP
                     GDWPAGTVLKVTYHYDDDQTVFVEVHDSDNHLLGTFEVERQANLDKSAVEVASRKIKD
                     LTID"
     gene            2169..3536
                     /locus_tag="VW324_002000"
     CDS             2169..3536
                     /locus_tag="VW324_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9727554.1"
                     /translation="MSVINYYEELGISETSSLDDVKKSIKSNRRRYRQLTGSPNIDQR
                     SMAERKMEVIAQAEKVFESEETRQKYDRELENSKQSSEGVPDSTPTNHSNSSYLDSAR
                     QAFYSGKKSLAYSYIEEALKINRNDADVWYFKAMISLEDRKLSDAELAISEANRLRPK
                     NADILSLLGDVYCEQNQQKFAIQYYQEAFELSNNSFYLLKKGRSLFLFDQYKQAVKDV
                     RYLYDHYLEENKNNEEYIYYLLSCSYREIGDNEEAYNFARKLVDVSDSLEHKLHYAGI
                     LFLKSEDECENYLDTLNERYGNNKDFQQVYFKLLLDITIERMKKAPDTDVEAFFRKYI
                     DRTKYENEAVLDNKARVRIAEAYLAKAQSLGIENKIIDNDFYNEFIENKNYINYATTR
                     HSQGMGCLMFILVFAANWFLSGFFDKFVHSFWISNALSLALLSNIVLYYNYPKGWQIN
                     RRKKG"
     gene            3544..4002
                     /gene="grpE"
                     /locus_tag="VW324_002001"
     CDS             3544..4002
                     /gene="grpE"
                     /locus_tag="VW324_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9727555.1"
                     /translation="MSEEVLHEKVSYIVDKVEDIESLITRRLLEDKAKNSLIEELKQY
                     LIYRQDLDKGEKFAPFMKQILQVIDRIESSEEKSDLLTSIAEELLQILSLNGLQVIDN
                     SGMIDPSMHEVVNTVAVTDEQSENNIVEVLQKGYLLNNRVLRPSKVTIAK"
     gene            4027..4839
                     /locus_tag="VW324_002002"
     CDS             4027..4839
                     /locus_tag="VW324_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEU9727556.1"
                     /translation="MLRFFKHRRNILYFILGFLWGRRQNAKVSPEPPTPSTPKHSELP
                     SISKATHNGKMTGFEPQKLKTYQLYQHELMFGEPGKGLNKSGFDESAVNLGQEGEINF
                     AKALQKKGLLEKLVTFWSVHNLNLEDERVDADIDCVIVSGSTIWLVDLKFYASGNVIY
                     READGLLYTIDSATGAQIGRPKKMSPNMSYAEESFSHKFASLLKYYRLETRVVLMPTY
                     KGAGRLDNVFWPGHIKAVSLEEMLDELSREDKFRDTIGGQMIRQTFNLLLKR"
BASE COUNT         1770 a          631 c         1007 g         1462 t
ORIGIN      
        1 ataaatacgc ttttcgtaaa tcgagcgaag gctatgctat actgtaaccg taaacaataa
       61 gatataaaag gagattgttg atgattaaat acaaagaatt gaaaagttat atagatgagt
      121 ggagatatta ttccgatgag aacaatccaa gattggattt agaatattgc aagacgaaaa
      181 ttgttgagaa agcaaaagag tttgatcttc cttgtcaaat tgacgaagaa caaataaaac
      241 tcggcggttt gttcaataaa gagattgaag aatgtctagt tatttcgcac ccaaatcatc
      301 agaaagacta tgtaaagttt tgttttcgtt taaaaaatca aggatcagta caacttctga
      361 ctatagatac tttaggtgag agtaaacaat taaaaaaata ctatatatca gaagataata
      421 aaagattcag agaagcaatt agagaatcgg acttgtcatt gggtcagaaa ttaggagctc
      481 aacttagtaa cttaacagtt agcggtctta gaactttggg gaaaaatcag agcaagattg
      541 atgcagaatt acaatactgt gagtttttaa cagaggtact aacacagttt agatcaaata
      601 atgattagga gtgaaatatg agcaaagcaa ttggaattga tttaggaacg acttattcag
      661 cagtatctgt attatcagag actggacaac cacaaatttt attgaatcaa gatggtgaaa
      721 atttaactcc atctgttgta ttctttcagg attttgatgg gaaagatgaa ccacttgttg
      781 gtattcaagc taaaaatttg gcagcaagta gacctgaagc agttgtacag tatgtcaaaa
      841 gacaaatggg aaatccaaat tggaagtttg attctcctag tgatactgtt tatacagcag
      901 aggaaatttc agcaatcatt ttaaaaagat taaaagaagg agcagaaaat gcccttggtg
      961 acaaagtaga ggatgttgta ataactgttc cagcttattt tgatgatgcc cgtagaacag
     1021 caacgaaaca tgctggagaa attgctggac ttaatgtttt acgagtttta aatgaaccga
     1081 cagccgctgc acttgcatac ggtatatcag cagaaaaaaa tgagacagta ttggtatatg
     1141 atttaggtgg tggaactttt gacgtcacat taatgaaaat aaaagatggc gagtttgatg
     1201 ttattgccac cgatggagat cggaatctag gagggtttga ttttgataac gccttatcaa
     1261 tgattattgc tgaaaagatg gaagaacagg gagcagaaga tatttatacg gatgaacatt
     1321 ttactgcctt acttcgtgaa aaatcagaga atacaaagag aggactaaca actgtagaaa
     1381 aaacaaatgt ctttctcgac tataaaggaa aaagttataa aattccgata acacgtgtag
     1441 aatttgaaga agcaacaaaa agtctaatga atcgtacaga ggagctgtta gatgatgttg
     1501 ttgaagagtc tggaatgtca tgggatgaaa ttgaccaagt actattaata ggtggttcaa
     1561 ctcgaatgcc aatggtacaa agaaaattgg aagagaaaat tgggaagaag attgtatatt
     1621 ctattaatcc agatgaagct gtagcacaag gagcagctat tcaagcagca ttggaaatcg
     1681 ctaataaatc agaaagtgca gatgttagtg aaacagttcg tggattagca gaaaagttag
     1741 ttgtttcgga tgtcacttct caagccttgg gaactctggc tctttcgaat ggtgtgaaaa
     1801 gaaatacaat tattattcct aaaaattcca aggtaccaaa caagtactct gagtattttt
     1861 caacagttgt agataatcaa caaaatgtgt tagtagaggt tactcaaggg gatgacgagg
     1921 atccgcaatt tgttacggtt ataggagaga gtaccattaa attaccaggt gactggcctg
     1981 caggaacggt tttgaaggta acttatcatt atgatgacga tcaaacagtt tttgtagagg
     2041 tacatgattc ggataatcac ttattaggga cttttgaagt agagagacaa gcaaatctcg
     2101 ataaaagtgc tgtagaagta gctagtcgta aaataaaaga tttaactatt gattagggga
     2161 ggaacacaat gtcggtcata aactattatg aagagttagg gatttcagaa acaagttcat
     2221 tagatgatgt taagaaaagt atcaagtcaa atagaagacg ctatcgccag ttgacaggtt
     2281 caccaaatat tgatcaaaga tcaatggctg agagaaagat ggaagtcatt gcacaggcag
     2341 aaaaagtatt tgaaagtgaa gaaaccagac aaaaatatga tagggaatta gaaaattcta
     2401 aacaaagttc tgaaggagtc cctgattcga ctccaactaa tcattcaaat tcaagctatc
     2461 ttgattcagc tagacaggcc ttttattcag gaaagaaatc attagcttat tcctacattg
     2521 aagaagcctt aaaaataaat cgaaatgatg cagatgtttg gtatttcaag gctatgatta
     2581 gccttgaaga tagaaagtta tcagatgcag aattagctat tagtgaagct aatagattga
     2641 gacctaaaaa tgcggatatc ttatctttgc taggtgatgt ttattgtgaa cagaatcagc
     2701 aaaagtttgc aattcagtat tatcaggaag cttttgaact atctaataat tccttttatc
     2761 ttttgaaaaa gggaagatca ctatttttat ttgatcagta taaacaggca gtaaaagatg
     2821 tgcgatatct gtatgatcat tatttagaag aaaataaaaa taatgaagaa tatatttatt
     2881 accttttatc atgctcttat agagaaattg gagataatga agaagcctat aattttgcaa
     2941 gaaaattagt agatgtttca gattcactag agcataaact acattatgca ggtattctat
     3001 ttttgaaatc tgaagatgag tgtgagaatt atttagatac cttaaacgaa cgctatggta
     3061 acaataaaga tttccagcaa gtgtatttta agttactatt agatatcacg atagagcgta
     3121 tgaaaaaagc acctgataca gacgttgagg ctttctttag aaaatatatt gatcgaacaa
     3181 aatatgagaa tgaagcagtt ctagataata aggcgcgtgt tcgcattgca gaagcttact
     3241 tggccaaggc acaaagttta gggattgaaa ataaaattat agataatgat ttttacaatg
     3301 aatttattga aaataaaaat tatatcaatt atgccacaac acgccattca caaggtatgg
     3361 gatgtctcat gttcatctta gtttttgcag ccaattggtt tttgagtggt ttctttgata
     3421 agtttgtaca tagcttttgg attagtaacg cattaagttt ggcattgtta tcaaatattg
     3481 tcttgtatta caattaccct aaaggatggc aaataaatag aagaaagaaa ggataaagtt
     3541 tttatgagtg aagaagtatt gcatgaaaaa gtttcgtata tcgtagataa agttgaagat
     3601 atcgaatctt taattacacg ccgtttattg gaagataaag ccaaaaatag tctgattgaa
     3661 gaattgaagc agtatttaat ctatagacag gatttagata agggtgagaa atttgctcca
     3721 tttatgaaac agattcttca agttattgat cgtattgaat catctgaaga aaaatcagat
     3781 ttgcttactt caattgctga agagcttctg caaattcttt cattgaatgg attacaagtt
     3841 attgataata gtggaatgat tgacccaagt atgcatgagg ttgttaatac agtagcagtt
     3901 actgatgaac aaagtgaaaa taatattgtt gaagtattgc aaaaaggata tttgttaaat
     3961 aacagggttc ttagaccaag taaagtgact attgctaaat aagatatttt ttaggaggtg
     4021 gagaaattgt tgcgcttttt taagcatcgc agaaacatac tatattttat attaggattt
     4081 ctttggggca gaagacaaaa tgctaaggtt tctccagaac cacctactcc ttccacacct
     4141 aaacatagtg aacttcccag tatttccaag gcaactcata atggtaaaat gacgggattt
     4201 gaaccacaaa aacttaagac ttatcaactt taccagcatg aattgatgtt tggagaacct
     4261 ggaaaaggat tgaataaatc aggattcgat gaatctgctg ttaatcttgg tcaagagggt
     4321 gaaatcaatt ttgctaaagc attacaaaaa aaagggcttt tagaaaaact agttaccttt
     4381 tggtctgttc ataatctcaa tttagaagat gaaagggtag atgcggatat tgattgtgtt
     4441 attgtttctg gatctacaat ttggttagta gatttaaaat tttatgcttc aggaaatgtg
     4501 atttatagag aagcggatgg tttattgtac actatagata gtgctacagg tgctcaaata
     4561 ggcagaccta aaaaaatgtc ccctaacatg tcttatgcag aagaaagctt ttcccataaa
     4621 tttgcgagtt tattaaaata ttatcgcttg gaaacacgtg ttgtcttaat gcctacctat
     4681 aagggtgcag gtagattgga taatgtcttt tggccaggtc atattaaggc tgttagtcta
     4741 gaagagatgt tagatgagtt atctagggaa gataagttta gggatacaat tggtgggcag
     4801 atgattcggc agacatttaa tttacttttg aaacgttaaa ttatgtaaaa atagtaaaga
     4861 attgatgttg
//
LOCUS       DAVKLW010000094         4750 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKLW010000094 DAVKLW010000000
VERSION     DAVKLW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.094"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(118..195)
                     /locus_tag="VW324_002003"
                     /pseudo
     CDS             complement(118..195)
                     /locus_tag="VW324_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            226..459
                     /locus_tag="VW324_002004"
     CDS             226..459
                     /locus_tag="VW324_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9727557.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            459..1793
                     /locus_tag="VW324_002005"
     CDS             459..1793
                     /locus_tag="VW324_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9727558.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1803..2057
                     /locus_tag="VW324_002006"
     CDS             1803..2057
                     /locus_tag="VW324_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9727559.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            2153..2548
                     /locus_tag="VW324_002007"
     CDS             2153..2548
                     /locus_tag="VW324_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9727560.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            2961..3410
                     /locus_tag="VW324_002008"
     CDS             2961..3410
                     /locus_tag="VW324_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9727561.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3397..3954
                     /locus_tag="VW324_002009"
     CDS             3397..3954
                     /locus_tag="VW324_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9727562.1"
                     /translation="MARTELPDQIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDSLIYGLLKSEWEE"
     gene            3956..>4750
                     /locus_tag="VW324_002010"
     CDS             3956..>4750
                     /locus_tag="VW324_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9727563.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESL"
BASE COUNT         1455 a          859 c         1087 g         1349 t
ORIGIN      
        1 ctcaaaacgg tgttttgagc tgacttcgtc agttctatcc acaacctcaa aacggtgttt
       61 tgagctgact tcgtcagttc tatccacaac ctcaaaacgg tgttttgagc tgacttcgtc
      121 agttctatct acaacctcaa aacactgttt tgagcaacct gcggctagct ttctagtttg
      181 ctctttgatt ttcattgagt attagaacat ataatggagg tcgtcatgga caatatcatc
      241 gatgtgtcaa ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta
      301 gtggagctgg gttttaaacc ccttgccaat cccttgatgc gcaatacagt tggtcgtaaa
      361 gtatcactta aacaaggttc taagctagca ggaactccta tggacaagat tgtacgcaca
      421 ctggaagcga atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg
      481 tcctacggga tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc
      541 gttttgatgc gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc
      601 tgatgaactc agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg
      661 ccaatctttt taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc
      721 acccagttcg tgtcttcaaa gaagaaaatc tggctctccg tgcggccttg attcgcattc
      781 gtagattgtt agatacctat gagtctatgg aagacgagga aatgctggcg gagatgcgta
      841 agggtttggt gcgtcagatg ggacttgtgg gtcaatttga catccattac caacgtaagg
      901 aagaactctt ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt
      961 ggggagtgga tgatcagatt agggaactct ttcaaacagc tctaacgata gccaagtcac
     1021 taccagaagt gtcaattagc agtgtaaagg aagcttttga agcttttgcg acagagtttg
     1081 aaagtatgat tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc
     1141 aggatgactg gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc
     1201 cgtcagagaa atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc
     1261 ctgtacagct agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc
     1321 attttaccat tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac
     1381 aggcttttgg taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcacctcc
     1441 ctatggagat tacctttgtc aataaagatg atattttcca gtattacaat gacaatacgc
     1501 cagctgatga gatgattttc aaacggacgc cgtctcaagt cgggcgcaat gtcgaactct
     1561 gccatccgcc taagtacttg gacaaggtca aaactatcat gaaggggctt cgtgagggga
     1621 ccaaggacaa gtatgaaatg tggttcaagt ctgagtcgcg aggtaagttt gtccacatca
     1681 cctatgctgc agtacacgat gaagacggag aattccaagg agtgttggag tatgttcagg
     1741 atatccagcc ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa
     1801 aaatgagtta cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca
     1861 tgattaacga catggcgcac aaggaaagtc aaaaagttta cgaagaagac caggacgagc
     1921 gtgccaaaga tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg
     1981 gtaagtttga aaacttcaaa gcaggcaagg gattccatga tttgccagaa ggcttgtttg
     2041 gtgagcgaaa ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg
     2101 tttatgttaa atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa
     2161 gaaactcaat cgtaaaaaac aattacgaaa tggcctccgt cgcgcaggtg ccttttcaag
     2221 tactgtgact aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc
     2281 aagcgcagct ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga
     2341 gcaagctcaa aaagtagcta attctgtaga agattttgca gcaaacttgg gtggacttcc
     2401 acttgatcgt gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa
     2461 ctggactgaa aaagaactcc ttgccttgaa aggaatcggc ccagctacca tcaagaaatt
     2521 gaaagaaaat ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgagaaaaac
     2581 ttgctacaat agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg
     2641 ctgagaagtc cacaaatgtg tcaaaactgg ttgctaatga ctgaaaaaat gaaataaaag
     2701 tgtctttttg ctttaaagac gagagttgcg ggcaactgcc cgaaattggg tggtaccgcg
     2761 gataaacaca ttcgtccctg tcatgtagat ggcagggctt ttcttttacg tcttagtcaa
     2821 agggggttgt tatgaagcaa tcttttaaca caagtaaact atattatggt tttcctgttt
     2881 ttattttagg atatcaagat tagaattttg gctataacat cacgacctgt agttcctctt
     2941 atagtctagg agattgggtt gtgattggag tcgttgcgag agagaatgcc gcagagcaga
     3001 tcaaacagta tcaaaaattt actgtgaata tttctgatga aacttctatg cttgcgatgg
     3061 agcaggctgg ttttatcagt catcaggaga aattggaacg tttgggagtg cattatgaaa
     3121 tttctgaacg aactcagact cctattttag acgcctgtcc acttgtttta gattgtaggg
     3181 tagataggat tgttgaggaa gacggtattt gccacatctt tgccaagatt cttgagcgac
     3241 ttgttgcccc agaactcctg gatgaaaagg gacattttaa aaatcaactg tttgccccaa
     3301 cctatttcat gggagatgga tatcagcgcg tttatcgcta tctggataag cgtgtagata
     3361 tgaagggcag tttcatcaaa aaagcgagga agaaggatgg caagaactga gctgccagat
     3421 caaatcgaaa cagaacgtct cgttttacga gtccgtactg tggcggatgc tgaggatatc
     3481 tttgactatg ctagtttgcc agaggtcgcc tatccagcag gttttcctcc agtcaagacc
     3541 ttggaagatg agatttatta tctggagcac attcttccgg agcgtaatca aaaggaaaat
     3601 ctcccagcag gctacgggat tgtcgtcaaa ggaacggata agatcgttgg ctctgtcgat
     3661 ttcaaccatc gccatgaaga tgatgtgctg gaaatcggct ataccttaca cccagactat
     3721 tgggggcgag gttatgtgcc agaagctgcg cgtgccttga ttgacttagc ctttaaagat
     3781 ttgggtcttc acaagattga actaacttgc tttggatata accttcaaag taaacgagtc
     3841 gcggaaaagc ttggctttac cctcgaagct cgcataagag accgcaaaga tgctcaaggc
     3901 aactgctgtg acagtctgat atatggcttg ctgaagagcg agtgggagga ataagatgag
     3961 cgatgtaaaa gaggaagtaa gctctctatc tgagaagcag ttacgccaaa ttgatgttga
     4021 gtatgctgaa ttaaatgata gtgatattat cgagcgatta gcttatttag aaattaacaa
     4081 caatgaaaaa cgtattgtca tctctgatat tgaaccaacc aaggaaataa tgtcagtctc
     4141 ggaccaaatt tttgagattc aaaagaattt tcaaaaaatc aagaatatgt tcgaattgtt
     4201 tatttcagat gtctcggatt ttttaagtat taaaaataaa cttgagtcaa aggaattaga
     4261 aattgaagag gcagatgtaa atcgtttcat gattcattta ttatccagtg gaaaattgtt
     4321 tgtcgatttt aacgaaaacc aaattaaaca aaaatattcc aaagatagtg aggagtttga
     4381 ttgtatacat ggttttgcaa gctatcagta tgataccaat tttgcttatc gattttgcca
     4441 ttctttgaga aattattcgc aacatactga cttaccaata aatgaggtaa aagctgttag
     4501 cccagatgat gagactgtta taatagattt ctatattgat ttagattatc ttttaaattc
     4561 caactttaaa tggaaaaaat tgaaagggga gttgataaaa ctaaaccaag aaacatctaa
     4621 gattgacgct atagctctag tgaaagaata ctttaatgct cttacagagc tctatggtaa
     4681 ttataataag ttgtttttaa agttaaacca taatacatta gtagatatta aatcaaagtt
     4741 ggaatcatta
//
LOCUS       DAVKLW010000095         4738 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKLW010000095 DAVKLW010000000
VERSION     DAVKLW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.095"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            28..1573
                     /locus_tag="VW324_002011"
     rRNA            28..1573
                     /locus_tag="VW324_002011"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1625..1697
                     /locus_tag="VW324_002012"
     tRNA            1625..1697
                     /locus_tag="VW324_002012"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1658..1660,aa:Ala,seq:tgc)
     gene            1822..4723
                     /locus_tag="VW324_002013"
     rRNA            1822..4723
                     /locus_tag="VW324_002013"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1313 a          973 c         1410 g         1042 t
ORIGIN      
        1 tgacagaaat gagtgagaac tcaaactttt aatgagagtt tgatcctggc tcaggacgaa
       61 cgctggcggc gtgcctaata catgcaagta gaacgctgaa ggaggagctt gcttctctgg
      121 atgagttgcg aacgggtgag taacgcgtag gtaacctgcc tggtagcggg ggataactat
      181 tggaaacgat agctaatacc gcataagagt ggatgttgca tgacatttgc ttaaaaggtg
      241 cacttgcatc actaccagat ggacctgcgt tgtattagct agttggtggg gtaacggctc
      301 accaaggcga cgatacatag ccgacctgag agggtgatcg gccacactgg gactgagaca
      361 cggcccagac tcctacggga ggcagcagta gggaatcttc ggcaatggac ggaagtctga
      421 ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaagctctgt tgtaagagaa
      481 gaacgagtgt gagagtggaa agttcacact gtgacggtat cttaccagaa agggacggct
      541 aactacgtgc cagcagccgc ggtaatacgt aggtcccgag cgttgtccgg atttattggg
      601 cgtaaagcga gcgcaggcgg ttagataagt ctgaagttaa aggctgtggc ttaaccatag
      661 taggctttgg aaactgttta acttgagtgc aagaggggag agtggaattc catgtgtagc
      721 ggtgaaatgc gtagatatat ggaggaacac cggtggcgaa agcggctctc tggcttgtaa
      781 ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      841 ctgtaaacga tgagtgctag gtgttagacc ctttccgggg tttagtgccg tagctaacgc
      901 attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg
      961 gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg
     1021 tcttgacatc cctctgaccg ctctagagat agagttttcc ttcgggacag aggtgacagg
     1081 tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg
     1141 caacccctat tgttagttgc catcatttag ttgggcactc tagcgagact gccggtaata
     1201 aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca
     1261 cgtgctacaa tggctggtac aacgagtcgc aagccggtga cggcaagcta atctcttaaa
     1321 gccagtctca gttcggattg taggctgcaa ctcgcctaca tgaagtcgga atcgctagta
     1381 atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
     1441 accacgagag tttgtaacac ccgaagtcgg tgaggtaacc gtaaggagcc agccgcctaa
     1501 ggtgggatag atgattgggg tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg
     1561 gatcacctcc tttctaagga taaggaactg cgcattggtc ttgtttagtc ttgagaggtc
     1621 ttgtggggcc ttagctcagc tgggagagcg cctgctttgc acgcaggagg tcagcggttc
     1681 gatcccgcta ggctccattg gtgagagatc accaagtaat gcacattgaa aattgaatat
     1741 ctatatcaaa tagtaacaag aaaataaacc gaaaacgctg tagtattaat aagagtttat
     1801 gactgaaagg tcagaaaata aggttaagtt aataagggcg cacggtggat gccttggcac
     1861 taggagccga cgaaggacgt gacaaacgac gatatgcctt gggtagctgt aagtaagcga
     1921 tgatccaggg atttccgaat gggggaaccc aacaggtaat acctgttacc cacatctgtt
     1981 aaggatgtga ggaggaagac gcagtgaact gaaacatcta agtagctgca ggaagagaaa
     2041 gcaaaagcga ttgccttagt agcggcgagc gaaacggcag aagggcaaac cgaagagttt
     2101 actcttcggg gttgtaggac tgcaatgtgg actcaaagat tatagaagaa tgatttggga
     2161 agatcagcca aagagagtaa tagcctcgta tttaaaatag tctttgtacc tagcagtatc
     2221 ctgagtacgg cgggacacgt gaaatcccgt cggaatctgg gaggaccatc tcccaaccct
     2281 aaatactccc tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc
     2341 gggaggggag tgaaatagaa cctgaaaccg tgtgcctaca acaagttcga gcccgttaat
     2401 gggtgagagc gtgccttttg tagaatgaac cggcgagtta cgttatgatg cgaggttaag
     2461 ttgaagagac ggagccgtag ggaaaccgag tctgaatagg gcgccttagt atcatgacgt
     2521 agacccgaaa ccatgtgacc tacccatgag caggttgaag gtgcggtaag acgcactgga
     2581 ggaccgaacc agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc
     2641 aaacgaactt ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgacatta
     2701 gagattcttg gaggtagagc actgtttggg tgaggggtcc atcccggatt accaatctca
     2761 gataaactcc gaatgccaat gaattatggt cggcagtcag actgcgagtg ctaagatccg
     2821 tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaataatt gttaagtgga
     2881 aaaggatgtg gggttgcaca gacaactagg atgttagctt agaagcagct attcattcaa
     2941 agagtgcgta atagctcact agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa
     3001 caatttaccg aagctgtgga tacctttata ggtatggtag gagagcgttc tatgtgtgat
     3061 gaaggtatac cgtgaggagt gctggaacgc atagaagtga gaatgccggt atgagtagcg
     3121 aaagacaggt gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg
     3181 ccctgggtta gtcgggacct aaggagagac cgaaaggtgt atccgatgga caacaggttg
     3241 atattcctgt actagagtat gtagtgatgg agggacgcag taggctaact aaagcagacg
     3301 attggaagag tctgtctaag cagtgaggtg tgaattgagt caaatgctta attctataac
     3361 attgagctgt gatggggagc gaagtttagt agcgaagtta gtgacgtcac actgccaaga
     3421 aaagcttcta gcgtttaaac atactctacc cgtaccgcaa accgacacag gtagtcgagg
     3481 cgagtagcct caggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta
     3541 acttcgggag aaggggtgct gacttaaagt cagccgcagt gaataggccc aagcaactgt
     3601 ttatcaaaaa cacagctctc tgctaaatcg taagatgatg tatagggggt gacgcctgcc
     3661 cggtgctgga aggttaagag gagtgcttag cgtaagcgaa ggtatgaatt gaagccccag
     3721 taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc
     3781 cgacccgcac gaaaggcgta atgatttggg cactgtctca acgagagact cggtgaaatt
     3841 ttagtacctg tgaagatgca ggttacccgc gacaggacgg aaagacccca tggagcttta
     3901 ctgcagtttg atattgagtg tctgtaccac atgtacagga taggtaggag tctaagagat
     3961 cgggacgcca gtttcgaagg agacgctgtt gggatactac ccttgtgtta tggccactct
     4021 aacccagata ggtgatccct atcggagaca gtgtctgacg ggcagtttga ctggggcggt
     4081 cgcctcctaa aaggtaacgg aggcgcccaa aggttccctc agaatggttg gaaatcattc
     4141 gcagagtgta aaggtataag ggagcttgac tgcgagagct acaactcgag cagggacgaa
     4201 agtcgggctt agtgatccgg tggttccgta tggaagggcc atcgctcaac ggataaaagc
     4261 taccctgggg ataacaggct tatctccccc aagagttcac atcgacgggg aggtttggca
     4321 cctcgatgtc ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg
     4381 cccattaaag cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
     4441 gtcgcgggcg taggaaattt gagaggatct gctcctagta cgagaggacc agagtggact
     4501 taccgctggt gtaccagttg tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg
     4561 ataaacgctg aaagcatcta agtgtgaaac ccacctcaag atgagatttc ccatgattat
     4621 atatcagtaa gagccctgag agatgatcag gtagataggt tagaagtgga agtgtggcga
     4681 cacatgtagc ggactaatac taatagctcg aggacttatc caaagtaact gagaatat
//
LOCUS       DAVKLW010000096         4539 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKLW010000096 DAVKLW010000000
VERSION     DAVKLW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.096"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            15..839
                     /locus_tag="VW324_002014"
     CDS             15..839
                     /locus_tag="VW324_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9727564.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMLYFLGNLYL"
     gene            852..2018
                     /locus_tag="VW324_002015"
     CDS             852..2018
                     /locus_tag="VW324_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9727565.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            2526..4019
                     /locus_tag="VW324_002016"
     CDS             2526..4019
                     /locus_tag="VW324_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9727566.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            4032..>4539
                     /locus_tag="VW324_002017"
     CDS             4032..>4539
                     /locus_tag="VW324_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9727567.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLL"
BASE COUNT         1471 a          789 c          945 g         1334 t
ORIGIN      
        1 agagttttct gacaattaat caatcctact tttatctaaa aatgaaagaa cacaaactca
       61 aagttcctta tacaggtaag gagcgccgtg tacgtattct tcttcctaaa gattatgaga
      121 aagatacaga ccgttcctat cctgttgtat actttcatga cgggcaaaat gtttttaata
      181 gcaaagagtc tttcattgga cattcatgga agattatccc agctatcaaa cgaaatccgg
      241 atatcagtcg catgattgtc gttgctattg acaatgatgg tatggggcgg atgaatgagt
      301 atgcggcttg gaagttccaa gaatctccta tcccagagca gcagtttggt ggtaagggtg
      361 tggagtatgc tgagtttgtc atggaggtgg tcaagccttt tatcaagcac aaaacaggtt
      421 ggtttgatgg catgatgacg acaggttgct ctatgggagc ctatcatgca ctcaatttct
      481 tcctccagca tccagatgtc tttaccaaag tgattgctct cagtggtgtt tacgacgcac
      541 gtttctttgt cggcgattac tacaacgatg atgctattta ccaaaactcg ccagtagatt
      601 atatttggaa ccaaaacgat ggctggttta ttgaccgtta ccgtcaggca gagattgtgc
      661 tgtgtacggg gcttggagcc tgggaacaag atggtttacc atccttttac aagctcaaag
      721 aagcctttga ccagaaacaa attccagcct ggtttgctga atggggacat gatgtcgccc
      781 acgactggga atggtggcgt aaacaaatgc tttatttcct cggtaatctc tatttataaa
      841 aggagttacc tatgaattac cttgttattt ctccctacta tccacaaaac tttcaacagt
      901 ttaccatcga actagctaat aaaggcatca cagtcttggg aattggtcaa gagtcttacg
      961 agcaattgga tgagcccttg cgcaatagct tgaccgagta ttttcgtgtt gataatcttg
     1021 agaacataga tgaagtcaaa cgtgcagttg cttttctctt ttataaacat ggtccaattg
     1081 accgcatcga gtctcacaat gaatactggc ttgagctaga cgcaacactc agagaacaat
     1141 tcaatgtttt tggtgccaaa ccagaggatc tcaaaaagac gaaatataag tctgaaatga
     1201 agaaactttt caaaaaagca ggtgttcctg tggtacctgg agctgttatc aagacggaag
     1261 cagatgttga tcaagcagtg aaagaaatcg gtcttccaat gattgccaaa cctgataatg
     1321 gagtgggagc agccgcaacc tttaaacttg agacagaaga cgatatcaat cacttcaagc
     1381 aagaatggga ccattcaacc ctttatttct ttgaaaaatt tgtcacttcc agcgaaatct
     1441 gtacctttga cgggctcgtg gacaaggatg gaaagattgt cttctcaaca acctttgact
     1501 acgcctatac accgcttgac ctcatgattt ataagatgga caattcttat tatgtgctca
     1561 aggatatgga tcctaaactg cgcaagtatg gggaagcaat tgtcaaagaa tttggtatga
     1621 aagaacggtt tttccatatt gagttcttcc gtgaggggga cgattatatt accatcgagt
     1681 acaataaccg ccctgcaggt ggttttacca ttgatgttta taactttgct cattccttgg
     1741 acctttatcg tggctatgca gctattgtcg caggagagga gttcccggcg tcagactttg
     1801 aaactcagta ttgtttggct acttctcgcc gtgcaaatgc tcactatgtt tattcagaag
     1861 aggatttgct tgccaaatat agccagcagt tcaaggttaa aaaagtcatg ccagctgcct
     1921 tcgcggaact tcaaggagat tacctgtata tgctgacaac tccgagtcga caagaaatgg
     1981 atcagatgat tgctgatttc ggacaacgtc aagaataaga actatcggat taaggaaatt
     2041 cactccctta atccttttgt tttgtctggt aaaaaataag agcatcctaa caaggtagct
     2101 atcataaaac ttgttcgata actatttgaa gcaggattag gtggctagaa attgaatttt
     2161 aacatttcaa tttagtcata gtattgtgtt ttcgtatcct taaatcagct aaaaggatcc
     2221 atgacgacac ctatacgata tagttttcag gataccaaac cagtctatta atactcaatg
     2281 aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt
     2341 gtggatagaa ctgacagagt cagtatcata tacctacggc aaggtgaagc tgacgtggtt
     2401 tgaagagatt ttcgaagagt ataaaatatt caggtgacgc atagatatag ttaattgaag
     2461 ctttgtttga aatctgataa aataatgata ttactaagtt ttaaaaacta aagaaaaggg
     2521 aagatatgat tacaggcgaa ttaaaaaata aaatcgatca gctgtgggaa attctttgga
     2581 cagaaggaaa cgcaaatcct ttaacaaata ttgaacagtt gacttatctc ttatttatga
     2641 aagatttgga tagtgtcgag cttggacgtg aaagtgatgc tgaatttcta gggattcctt
     2701 atgagggagt ttttccaaaa gataaacctg aataccgttg gtcaactttt aaaaatatag
     2761 gagatgctca ggaagtttat cgtttaatga ctcaggagat ttttccgttt attaaaaatc
     2821 tcaaggggga tacagatgat acagcctttt cacgatatat gcgagaagct atttttcaaa
     2881 taaataaacc tgctacgctt caaaaggcaa tttctatctt agatgttttt ccaactaggg
     2941 gattagatgt agattttgat aatgacaaac aaagtattac tgatatcgga gatatctatg
     3001 aatatctgtt atcaaaattg tcgaccgcag gtaaaaatgg acagttccgt acacctcgtc
     3061 acatcatcga tatgatggtt gagttgatgc aaccgactat caaagatatc atctcagatc
     3121 ccgctatggg ttctgctggc ttcttagtat ctgctagccg ttacttaaag cgtaagaaag
     3181 atgaatggga aaccaataca gataatatca atcattttca taatcagatg tttcatggaa
     3241 atgatacgga tacgactatg ttgagacttg gggcgatgaa catgatgcta catggagtag
     3301 aaaatccaca aatcagttac cttgactcgc tgtctcaaga taatgaagaa gccgataaat
     3361 atactttggt tttagcaaat cctcctttta agggctcact tgactacaat tcaacctcta
     3421 atgaccttct tgcaaccgta aaaaccaaaa aaacagaatt actctttctt tctcttttct
     3481 tgcgaacttt aaaaccaggt ggacgagcag cagttatcgt acctgatggt gtcctttttg
     3541 gttcgtctaa agctcataaa ggaattcgtc aggaaattgt agagaatcat aagcttgatg
     3601 ctgtaatctc aatgcctagt ggtgtgttca agccttatgc tggagtttca actgccattc
     3661 tcatctttac aaaaactggt aatggtggta ctgacaaagt ctggttttac gatatgaaag
     3721 cggatggttt aagtttggat gataagcgac aaccgattag cgacaatgat attccagata
     3781 ttatcgaacg ctttcatcat cttgaaaaag aagcagaacg tcagagaacg gatcaatctt
     3841 tctttgttcc agttgctgag ataaaggaaa atgattatga tttgtctatc aataaatata
     3901 aagagattga gtatgaaaaa gttgagtatg aaccaacaga ggtcatctta aagaaaatca
     3961 atgatttaga aaaagaaatt caagctggct tggctgaatt agaaaaatta ctcaagtagg
     4021 gaggtggctg tatgaaaaaa gtgaagttgg gggaagtctt atctctaaaa aaaggcaaga
     4081 aagccactgt acttgctgaa caaacaactc taagccaacg ttatattcaa atagatgatt
     4141 taagaaataa taataattta aaattcactg aaagtttaaa tatgactgaa gcactcccag
     4201 atgatattct gatagcatgg gatggagcta atgcaggaac agttggttat ggattatcgg
     4261 gagctgttgg tagtacaatt acggtcttaa aaaagaatga gcgatacaaa gaaaaaatta
     4321 tatcagatta cttgggagtc tttttggaaa gtaaatcgca gtatttacga gaacattcaa
     4381 caggtgcaac aattcctcat ttaaacaaga atatattact tgatttacaa ttagaattgc
     4441 taggtatcga agaacaagag aacattatct gtattcttaa tacgattaaa gggcttatta
     4501 ctaaaagaaa attgcagtta gatgaactaa acttgctcg
//
LOCUS       DAVKLW010000097         4176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKLW010000097 DAVKLW010000000
VERSION     DAVKLW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.097"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            33..799
                     /locus_tag="VW324_002018"
                     /pseudo
     CDS             33..799
                     /locus_tag="VW324_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1618..1977
                     /locus_tag="VW324_002019"
     CDS             1618..1977
                     /locus_tag="VW324_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727568.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1987..2352
                     /locus_tag="VW324_002020"
     CDS             1987..2352
                     /locus_tag="VW324_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9727569.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            2339..>4176
                     /locus_tag="VW324_002021"
     CDS             2339..>4176
                     /locus_tag="VW324_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9727570.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKIYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYEEIARRLDLFVKIINTNKFTVL"
BASE COUNT         1519 a          623 c          823 g         1211 t
ORIGIN      
        1 ttaaaaagtt acaaatggca gtctcaatca taatgaaaaa ttatccgatt tatgatatac
       61 atcctcatat tttacatgga gtagaaatta gtgcagcaga taaattgcat attgtatgta
      121 tatatgatta tgaacaagaa tcatgggtta atcaatggtt aagtgaaaat attataagtg
      181 agaaagatgg aagttatcaa cattcactga ctataatgaa ggatttcaat aatcaaaaaa
      241 tagttaacta tattgctcat ttcaatagtt atgacatttt gaaaaaaggt tctcacttat
      301 caggtgcata taaacgaaaa attttttcta aagaaaatac acgattttgg agtttaatat
      361 taactcgaaa gaatcttcgc aacaacttga tattctctat aaagaagttg gtgtattaag
      421 tttgggacaa aaagttgtag ccatgcttga ttttttatta gcatatagtg attattctaa
      481 agacttcaga ccattgatta ttgatcagcc tgaagacaat ctagacaatc gttatatttg
      541 caggcattta gttcagcagt ttagagatgt gaaagctcaa cgtcaaatta ttttagcaac
      601 acataatgct acaattgtaa caaattctat gacagatcaa gttgttatta tggagtcaga
      661 tggagttaac ggatggattg aatcacaggg atatgttagt gaaaaatata taaaaaatca
      721 tatcatcaat caattagagg gaggaaaaga ttccttcaag cataaaatgt ctatatatga
      781 gacggcttta tcagagtaga gtcagaaaaa gtaggttaga aatttagcct acttttttct
      841 ttgtccgaca ggcatagtgt gcatctgagg tccaagtcct ctgtggatat ttgctgcaga
      901 tgaaaccaat agcgactcct aagcctgaat atcgtgaggt aggggggata ggaaggaatt
      961 agcgaaatca aggttctaca aacagaatcg tgacttgaag ccatatatag cggatgagga
     1021 actctaaaat ccaaataggt gtcgtaacct atatacgtaa attacgagag taaactagga
     1081 aagatgtacg gcttattccg tgagcgttta ggacgtagta caacgaatca tgggagtcag
     1141 ctgaacacat agtattgaag aaatttctgt aatggaaatg gagcgaagaa gtgaacaatt
     1201 aaatgaatac ctctctaatt aaatttgtca attctaattc ctggtatgaa aagacagtga
     1261 cctgaaaatg taaacgatgg gagctgatta taaatatagg acggtacatg cagtggtgtt
     1321 agagattagt ccctacttga tttgtgataa cttccccaaa tttcttctgc tatacttttc
     1381 tcaactttta aaaatccaac taagaatttt acctgggggt ttgggggcga agccaccaag
     1441 ttatcttatc gttggctgtc aaaactggaa ggttttggtt agctggcgat atgatttttt
     1501 gggtattgta accacaatgc ttgagctcgc aaagaccgaa caaggacttt aagaaatttc
     1561 ccgtatgaca cacggggtcg gggtttaccc gagaaaggat gaaagtgagg aaaagagatg
     1621 tctgaacagt accgtgacat tcgaaaagag gtaaatctaa ccgcagatga gctaaaacag
     1681 attgaaaaaa tgatggaggt tgataattat cgtcactttt caccattcgt cagggataaa
     1741 attttgatga ctgatgacaa acagttagct gccaaagaat ggttttccct ttggcagtct
     1801 caaaagtttg aacagatcag tcgagatgtg catttggttt tgattatcgc aagagagaat
     1861 caccaagtga ctcaagaaca cgtgtcaatc ttattgacct gcgttcaaga attgattgcg
     1921 gaagtcaatc aagtacagtc actcagtcgt gggtttcgtg aaaaatacat gagataggag
     1981 gctactatgg tttatcgtta tcgtaccaat ctcaaaaaag tatttctaac agattcagaa
     2041 ttacaccaat tgaatgaacg gattgctaag agcaattgtc aaaatttctc agtctatgcc
     2101 agaaaagttt tgcttaatcc caatatgtca tttgtcacga ttaacactga tacttatgaa
     2161 cagttagtgt ttgaattgag gaggattgga aataacatca atcaaattgc gcgtgcgatt
     2221 aatcaaagcc atctgatttc tcagaaccaa ttacaagaat tgagtaaagg agtcggagag
     2281 ttaattaagg aagtggacaa agaatttcaa gtggaggtga aaagactgaa ggagtttcat
     2341 ggtagttact aaacactttg cgacgcacgg aaaaaaatat cgtaggcgtt taattaagta
     2401 tattctcaac cctgataaaa cggacaattt gaaattggta tctgattttg gcatgagcaa
     2461 ttacttggac ttccctagtc atgcagaaat ggtagagatg tacaatgtca acttcagcaa
     2521 taatgacaag ttgtacgaat ccagaaacga ccgacaagaa aaacaccaac aaactattca
     2581 tgctcattac ctcattcaat cattttctcc tgaagataat ctgacacctg aagaaattaa
     2641 ccgcattggt tatgagatca tgatggaatt gacaggaggt cgttttaagt ttatcgtagc
     2701 aactcacaca gacaaagatc atgttcataa tcacatccta atcaacgcta ttgaccgcaa
     2761 ttcagataaa aagttgatat ggaactatgc cttggaacga aacctccgta tgatttcaga
     2821 ccgtatttct aaagtgactg gtgcaaaaat tattgagaaa cgttattcct atcgagatta
     2881 taaaaaatat agggaatcta gtcataagtt tgaattgaaa caacgtcttt attttttgct
     2941 acagcaatct aaatcgtttg atgatttttt agagaaagca aagcagttgc atgttcagat
     3001 tgattttagt caaaagcata gccgtttttt gatgacagat agaacgatga taaaaccaat
     3061 tcgaggacgc caactcagca aacgagattt atatgatgaa gactttttta aaatatattt
     3121 tgccaaacaa gagattgaaa gtcgtttaaa atttttacta aagtctgttt attcattaga
     3181 agaattgcat gtaaaggcta aagagttgaa tctaaccatc gagttaaaac agaaaaatgt
     3241 gatgtttacc ctagaagaag atggcaaaaa gataagcctg agtcataaaa aaattagcga
     3301 taagaaattg tacgatgttc aattttttaa tcgttatttt gaagatagag aagttagaga
     3361 tattcaagca ttagaaaatc ttcaggaaga ttttcagact tttcgagagg agcagcataa
     3421 ggagaaagtc tccgctgaag aaatcgagga agccttcaag aaatacaagg aaaaacgaga
     3481 tgctatccat gagtttgaga ttgaacttac ggataatcag atagagaagt tggttgatga
     3541 tggtgtctat atacaggttt cgtttggtat caaacagagt ggactcatct ttattcctaa
     3601 ctaccagcta gatatttttg aggaagacaa tcataaaaaa tataaagtct acattcgaga
     3661 aacgtcttct tactttgtct ataataaaga aaacatggat aataattgtt tcatcaaggg
     3721 taggacctta atcagacaat tgagcaacga tagccaaaaa ctcccctata gacgaccaac
     3781 tttaaaaagt cttaaagaga agatttctga aattaatctt atgattgaac tatctaacac
     3841 gaacaaacaa taccaagaaa tcaaagatga gcttgtatta gaaattgcag agattgatat
     3901 gcagctagaa gaaacccaag aaaaaatcgc caccttaaac aagatggcgg aagtccttat
     3961 caatttgaag agtgaagatc atgagactag aaaattagct aagtatgatt ttgaccaaat
     4021 gaacatgacg gaatcaataa tgttagatcg actcaatacg gatattttaa aattacaaca
     4081 ggaacttggt aatgaaatta ataaatatga ggagatagct agaagattgg atttgtttgt
     4141 gaaaattatt aataccaata aatttacagt tctaaa
//
LOCUS       DAVKLW010000098         4070 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKLW010000098 DAVKLW010000000
VERSION     DAVKLW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.098"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..127)
                     /locus_tag="VW324_002022"
     CDS             complement(<1..127)
                     /locus_tag="VW324_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9727571.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLK"
     gene            complement(313..924)
                     /gene="rpsD"
                     /locus_tag="VW324_002023"
     CDS             complement(313..924)
                     /gene="rpsD"
                     /locus_tag="VW324_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9727572.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1119..2171)
                     /locus_tag="VW324_002024"
     CDS             complement(1119..2171)
                     /locus_tag="VW324_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9727573.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2168..2866)
                     /locus_tag="VW324_002025"
     CDS             complement(2168..2866)
                     /locus_tag="VW324_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9727574.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(3033..>4070)
                     /gene="pavB"
                     /locus_tag="VW324_002026"
     CDS             complement(3033..>4070)
                     /gene="pavB"
                     /locus_tag="VW324_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9727575.1"
                     /translation="WSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALI
                     DQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVK
                     DSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAK
                     NPALSPLGENVKTKGQYFYQLALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKD
                     GKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPAD
                     TMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLG
                     GLGLKNKKEEN"
BASE COUNT         1096 a          900 c          801 g         1273 t
ORIGIN      
        1 cttttagctt taaccagcca taaatggtat tacgtgagat ttggaaaacg tgtgatgctt
       61 ctgttatact acctgttcgc tcacaataag agagaacttt tttacgaaaa tctattgaat
      121 atgccataaa aggattatac cacattgtgt actatttttg gttcatttta ctatatcact
      181 ttggactata tcggtctagt tatgtctcaa cgattatagc tggatactaa gagaaatcaa
      241 aaaagcacta agggagaacg tcccaaagtg cttaatatca aggcctcaaa gtctatctta
      301 ttcaataaaa tattacaaca ttttgttgta gaattcaacg acaagtgctt cgttgatttc
      361 tgggttgatt tcgtcgcgtt ctggcaagcg agtcaatgaa ccttccaact tttcagcgtc
      421 gaatgatacg aatgctggac gtccaagagt agcttctact gcttcaagga ttgctggaac
      481 tttcaatgat ttttcacgaa ctgagatcac ttgacctgga gttacacggt atgatgggat
      541 atcaacgcgt ttcccgtcaa caaggatgtg accgtggttt acgaattgac gagcttgacg
      601 acgagtagtc gcgagaccaa gacggtaaac aacgttatcc aaacgacgtt ccaaaagaag
      661 cataaagttg aaacctagga ttccgccttt gatttttgta gcttgtacga acaagttacg
      721 gaattgtttt tcacctacac cgtaagtgaa acgaagtttt tgtttttcag ccaattgcaa
      781 accgtattct gacaatttag aacggttgtt tggtccgtgt tgtcctggta cgtagttacg
      841 acgtgccaat tctttacctg tacctgtaag tgaaaggcca aggcgacgag cttgtttcca
      901 agatggtcct gtataacgtg acatatgaat gtcctcctga tataaataat atttcggtgg
      961 aaatagtcac ttagaaagcc ctgattcgtg cagatgccct tcgcctaaac agccaaggtt
     1021 acttgtcata agacacctgt tgacgagctt catgctttcc tgctgatatt tcacacaaaa
     1081 tctattctac catgaatagc tggatttgta aaggggtttt aggctttatt ttcactacca
     1141 gagaggttga gaacgagggt aaaggtactt cctagaccgt actggctgct gactgtgatt
     1201 tccccaccca attgatgggc caattcacgc gcaatcgcaa gtcctaatcc atgaccacct
     1261 gtcttcatgt tacgcgaagt ttcgacacga taaagacgtt tgaaaatatt ttccaaatcc
     1321 tctggggcaa taccctgccc ttcatcggtc acactgattg aaagctggtc cttctccagc
     1381 ttagccacca cttccagctt ggttcctgga gcagaatatt taaaagcgtt atcgaccaga
     1441 ttcaccaaga tacgagaaag cttagcataa tctccctcaa tccgggcaga ctctgggatt
     1501 acctgcaagt ggacatctct tctctcctgc tcaatcaaaa actgaaattc actcatgcac
     1561 tcaattaaga gcttgtccag aaaaatactg tctttactgg tagtttccac ctgattccta
     1621 gctgtgttta gggtcaaaaa attcaactcc tcaaccagtt tattgagcct ctccgtctgg
     1681 cgtccaatgg ttgctagata atgagcttgc tccgactcct tgataatccc atccaaaatc
     1741 ccttctaccg tcgcttggat cgaagtgata ggagtcttaa tatcatgcga caactgggca
     1801 atcatcaagc ccttttctcg ttcgctttct tccaaggaat caaagcttac ctgcaaatca
     1861 tgggacatct cattaaaagt ttgccctaat tgctgaaatt ctacaggacc ttgaacctcc
     1921 aaatttgaag gaaaatcctt ggccgctacc cgcttggcat gctccttgag tttgcccaac
     1981 gacgtaaaga ctggcaatag gagaaagaga ctaatcccag caccgacaag gctggcaacg
     2041 atggtcatcc caagcaaaaa gtaaatctcg cctttcgcaa tcagcatttt ttgaacagcc
     2101 caaaaaacaa ccaaaatggt taagagggtt gaaataatat atccaaccaa aatataactt
     2161 tttagtttca tgtttgtcct ctcggtttct ctatcttata tcccaacccc caaactgtct
     2221 taatagtggg cgtttggtca ctactatatt ttgccagctc ctgtcgaaga gcatggatat
     2281 gcacattcaa ggtattggtg tcatccacgt agtcttcttt ccagatcttt tcatagaggt
     2341 ctgtcttgga gaaaactcgc tctggattgc tagctaaaat ccacagcaat tcaaatgatt
     2401 taacagttaa atccagcatt tcttctccta tttgaacttc atgactacta tgattcattt
     2461 ttagattgcc aagggaaatc agctctgttt cgcccccacg atgaaggcgg cgcaaaatat
     2521 tgtggacacg caaaaccagc tcacgtgggc taaaaggctt agcaataaaa tcatctgctc
     2581 ccaagctcag gccgtaaatc ttgtcctgtt cactggtctt agcagtaata aataggaaag
     2641 gctgctctgg tgataagtat tgaacctcac tgattaaatc ataaccatcc atccgaggca
     2701 tcatgacatc tgtgataatc aaatcaatcg gttttttctt gaacagctct agcgcttcca
     2761 gtccatcatg ggctaccaag acctgataac ctgcctgaat taagtatctc tgatgaatat
     2821 ctgtgatttc tacctcgtcg tcaacgagta aaattgtctt tcccatctgt ctctcctttg
     2881 ataaaaacag tgctatactg ctttaagtat aacactattt ttattgtttt acgatgattt
     2941 taatctgcta ttgaaagaaa gtcctgattg ctgattgagt tgttaaacta atcttagtca
     3001 gcccactatc catcatttat ttccttagct gattagtttt cttctttttt gtttttcaaa
     3061 cctagaccac cgagtaaacc tgcaagcgca agcccaagga aaccaatact tgccattgat
     3121 gtttgagtct caccagtatt tggtagcata gctttatcct ctgacatcat cgtatcagac
     3181 atcttgttag cagaagcagc catgttttca cctgccatcg tgttggtaga acttgtcatg
     3241 gtgtcagcag gcatgctatc tgtagtacct gtagcatgat tgtgattcat cggagtcacg
     3301 ccagaaccag agttagaagg tgataatgaa ccatttgctg tgtctgatgt ttctttaaca
     3361 tttatcttaa tagtgacttt tttagttgct acgatgttgt ccaaatctgg tttaccgtct
     3421 ttgttaccat agacattgac tgtagcgctg taagtttgag taccgtttgc tcggaactgg
     3481 tcaatgagcg cttgtttttc tttgccagct acatttccgt ccaaggctaa ttgatagaag
     3541 tattgacctt tggtcttcac gttttcacct agtggagata gggctgggtt tttagcgtca
     3601 ccgttatctg accatggtgc cttgtcagat gccttgagca agagacgagt caacatacca
     3661 tcacctgcga agagttcgta tggaatcaca tggttgacac ctgctgtgaa tggaccttca
     3721 cccttggctt tttctaggta ggctgctgga acatcgatac tgtccttaac gttgtctgca
     3781 acggcttttt gaactgtttc tttagaaatt aaaccgttta tgttaatagt gactttttta
     3841 gttgctacga tgttgtccaa atctggttta ccgtctttgt taccatagac attgactgta
     3901 gcgctgtaag tttgagtacc atttgctcgg aactggtcaa tgagcgcttg tttttctttg
     3961 ccagctacat ttccgtccaa ggctacttga tagaagtatt gacctttggt cttcacgttt
     4021 tcacctagtg gagatagggc tgggttttta gcgtcaccgt tatctgacca
//
LOCUS       DAVKLW010000099         3810 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKLW010000099 DAVKLW010000000
VERSION     DAVKLW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.099"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..418
                     /locus_tag="VW324_002027"
     CDS             <1..418
                     /locus_tag="VW324_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9727576.1"
                     /translation="KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDETEIPQVENSVINAKIAEAEALLEKVTDSSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VW324_002028"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VW324_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9727577.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLVGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     SEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSETGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1429 a          622 c          747 g         1012 t
ORIGIN      
        1 taaaaataag gcagaccaag acagtaaacc tgatgaagat aagggacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 tacagatgag actgaaattc ctcaagtaga gaattctgtt attaacgcta agatagcaga
      241 ggcggaggcc ttgctagaaa aagtaacaga ttctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa aaaaagtcaa ccggctccga tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggttgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatctct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aattgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcagcctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaac tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact
//
LOCUS       DAVKLW010000100         3735 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKLW010000100 DAVKLW010000000
VERSION     DAVKLW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.100"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            81..308
                     /locus_tag="VW324_002029"
     CDS             81..308
                     /locus_tag="VW324_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727578.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            596..3541
                     /locus_tag="VW324_002030"
     CDS             596..3541
                     /locus_tag="VW324_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9727579.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSEEGENITLPAEHVESVSETELSGNKQEQERKDK
                     QEEKIPRDYYARDLENVETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFK
                     PDAKAPAFYNLFSVSSATKKDEYFTMAVYNNTATLEGRGSDGQQFYGNYNDAPLKVKP
                     GQWNSVTFTVEKPTAELPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRA
                     NNTVWGSNLQIRNLTVYNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESG
                     RNGKPNKDGIKSYRIPALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWG
                     DKVVISNLRDNPEAKDPAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTP
                     EKAYEKIGDKTYQILYKQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGN
                     LYKGQELIGNIYFAHSTKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKF
                     LGTGPGTGIVLRNGPHKGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVN
                     DNRQVDGQKIHSSTMNNRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTW
                     EKDIKRYPQVKDVYVQMSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTW
                     LKHNPIQKGEFAYNSLQELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKNLISPT
                     EAKVKRTREMGKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFA
                     VDKEDIGQEIIGIAKGSIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGIE
                     PAVHEIAEYKGSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGL
                     FTLGKKREQ"
BASE COUNT         1278 a          607 c          861 g          989 t
ORIGIN      
        1 tagattgctg taaaaatagt agtagctatg cgaaataaca gatagagaga agggattgaa
       61 gcttagaaaa ggggaataat atgatattta aggcattcaa gacaaaaaag cagagaaaaa
      121 gacaagttga actacttttg acagtttttt tcgacagttt tctgattgat ttatttcttc
      181 acttatttgg gattgtcccc tttaagctgg ataagattct gattgtgagc ttgattatat
      241 ttcccattat ttctacaagt atttatgctt atgaaaagct atttgaaaaa gtgttcgata
      301 aggattgagc aggaagtatg gtgtaaatag cataagctga tgtccatcat ttgcttataa
      361 agagatattt tagtttaatt gcagtggtgt cctggtagat aaactagatt ggcaggagtc
      421 tgattggaga aaggagaggg gaaaattggc accaatttga gatagtttgt ttagttcatt
      481 tttgtcattt aaatgaactg tagtaaaaga aagttaataa aagacaaact aagtgcattt
      541 tctggagtaa atgtcttatt tcagaaatcg ggatatagat atagagagga tcagtatgaa
      601 tcggagtgtt caagaacgta agtgtcgtta tagcattagg aaactatcgg taggagcggt
      661 ttctatgatt gtaggagcag tggtatttgg aacgtctcct gttttagctc aagaaggggc
      721 aagtgagcaa cctctggcaa atgaaactca actttcgggg gggagctcaa ccctaactga
      781 tacagaaaag agccagcctt ctgaggaagg agaaaatata actcttcctg cagagcatgt
      841 agagtctgtt tcagagactg aactttctgg caataagcaa gaacaagaaa ggaaagataa
      901 gcaagaagaa aaaattccaa gagattacta tgcacgagat ttggaaaatg tcgaaacagt
      961 gatagaaaaa gaagatgttg aaaccaatgc ttcaaatggt cagagagttg atttatcaag
     1021 tgaactagat aaactaaaga aacttgaaaa cgcaacagtt cacatggagt ttaagccaga
     1081 tgccaaggcc ccagcattct ataatctctt ttctgtgtca agtgctacta aaaaagatga
     1141 gtacttcact atggcagttt acaataatac tgctactcta gaggggcgtg gttcggatgg
     1201 gcaacagttt tacggtaatt acaacgatgc acccttaaaa gttaaaccag gtcagtggaa
     1261 ttctgtgact ttcacagttg aaaaaccgac agcagaacta cctaaaggcc gagtgcgcct
     1321 ctacgtaaac ggggtattat ctcgaacaag tctgaaatct ggcaatttca ttaaagatat
     1381 gccagatgta acgcatgtgc aaatcggagc aaccaagcgt gccaacaata cggtttgggg
     1441 gtcaaatcta cagattcgga atctcactgt gtataatcgt gctttaacac cagaagaggt
     1501 acaaaaacgt agtcaacttt ttaaacgctc agatttagaa aaaaaactac ctgaaggagc
     1561 ggttttaaca gagaaaacgg acatattcga aagcgggcgt aacggtaaac caaataaaga
     1621 tggaatcaag agttatcgta ttccagcact tctcaagaca gataaaggaa ctttgatcgc
     1681 aggtgcagat gaacgccgtc tccattcgag tgactggggt gatatcggta tggtcatcag
     1741 acgtagtgaa gataatggaa agacatgggg agataaggtg gttatctcca atcttcgaga
     1801 taatcctgaa gctaaagatc ctgctgcgcc atcgcctcta aatattgata tggttttggt
     1861 tcaagacccg acaacaaaga gaatcttctc aatttatgat atgttcccag aaggtcgagc
     1921 agtttttgga atgccaaaaa cacctgaaaa agcttatgaa aagatagggg ataaaactta
     1981 tcaaatcttg tataaacaag gagagtctgg tcattatact gttcgtgaga atggagaagt
     2041 gtataatgca caaaatcaaa agacggatta tcgtgttgta gtgaatccaa cagaacctgg
     2101 ctatagagat aaaggaaatc tttacaaagg tcaggaattg attggaaata tctattttgc
     2161 acacagtaca aaaaatccat ttagagtagc caatacgagc tatctatgga tgtcatatag
     2221 tgacgatgat ggtaaaactt ggtctgcacc gagagatatt actccaggtc ttcgcaagga
     2281 ttggatgaag ttcctaggaa caggtcctgg aacaggaatt gtacttcgga atgggcctca
     2341 caagggacgg attttgatac cggtttatac gactaataat gtatctcact tagatggctc
     2401 gcaatcttct cgtgtcatct attcagatga tcatggaaaa acttggcatg ctggagaagc
     2461 ggtcaacgat aaccgtcagg tagacggtca aaagatccac tcttctacga tgaacaatag
     2521 acgtgcgcaa aatacagaat caacggtggt acaactaaac aatggagatg ttaaactctt
     2581 tatgcgtggt ttgactggag atcttcaggt tgctacaagt aaagacggag gagtgacttg
     2641 ggagaaggat atcaaacgtt atccacaggt taaagatgtc tatgttcaaa tgtctgctat
     2701 ccatacgatg cacgaaggaa aagaatacat tatcctcagc aatgcaggtg gaccgaaacg
     2761 tgaaaatggg atggtccact tggcacgtgt cgaagaaaat ggtgagttga cttggctcaa
     2821 acacaatcca attcaaaaag gagagtttgc ctataattcg ctccaagaat taggaaatgg
     2881 ggagtatggt atcttgtatg aacatactga aaaaggacaa aatgcctata ccctatcatt
     2941 tagaaaattt aattgggact ttttgagcaa aaatctgatt tctcctaccg aagcgaaagt
     3001 gaagcgaact agagagatgg gcaaaggaga gatgggcaaa ggagttattg gcttggagtt
     3061 cgactcagaa gtattggtca acaaggctcc aacccttcaa ttggcaaatg gtaaaacagc
     3121 gactttccta acccagtatg atagcaagac cttgttgttt gcagtagata aggaagatat
     3181 cggacaggaa attattggta tagctaaagg aagcatcgaa agtatgcata atcttcctgt
     3241 aaatctagca ggtgccagag ttcctggcgg agtaaatggt agcaaagcag cggtgcatga
     3301 agttccagaa tttacagggg gagttaatgg tatagagcca gctgttcatg aaatcgcaga
     3361 gtataaggga tctgattcgc ttgtaactct tactacaaaa gaagattata cttacaaagc
     3421 tcctcttgct cagcaggcac ttcctgaaac aggaaacaag gagagtgacc tcctagcttc
     3481 actaggacta acagctttct tccttggtct gtttacgcta gggaaaaaga gagaacaata
     3541 agagaagaat tctaaacatt tgattttgta aaaatagaag gagatagcag gtttttaagc
     3601 ctgctatctt tttttgatga cattcaggct gatacgaaat cataagaggt ctgaaactac
     3661 tttcagagta gtctgttcta taaaatatag tagattgaaa taagatgtga acaactctat
     3721 caggaaagtc aaatt
//
LOCUS       DAVKLW010000101         3487 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKLW010000101 DAVKLW010000000
VERSION     DAVKLW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.101"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            805..2964
                     /gene="pfbA"
                     /locus_tag="VW324_002031"
     CDS             805..2964
                     /gene="pfbA"
                     /locus_tag="VW324_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9727580.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTIKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNGNGKETVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNIQINDKIDNKQDNKTEEVNHKIVGDGIETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGISLLLTLKGLKYYGKDK"
     gene            3123..3239
                     /locus_tag="VW324_002032"
                     /pseudo
     CDS             3123..3239
                     /locus_tag="VW324_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
BASE COUNT         1330 a          439 c          638 g         1080 t
ORIGIN      
        1 aatattgttc aaagttttgt gcctgtcttt catgttctag tcattctttt gcatgataga
       61 atttatagca tattgatatt ataataatac aaatattcta tacgtttagt gatgcttgct
      121 atacatcatt agatctcccg cgagacaatc tataaaacac ttgtctacga ttacctatat
      181 gccctattcc agtattttag aagcactgca tctattttta tcgaggttaa atctagcttt
      241 tatagaaggt ctatttaaga aatatattgt agtgttttag tttcaatccg ccatatgagc
      301 gatattcagg taaatatccc tagcgaatgc ttgtatgaca agatatttgt tctttcattt
      361 ataatttaca acatatcaac aaatttaaat atagtaaatg ggatatttta tattcaagct
      421 aagaaagata gcatcacttt tgaatggaag gctaaagagc aaactaggaa gttggccata
      481 gatagctcaa aaccctgctt tgaggttgta gatataataa aatgaaataa gaataggaca
      541 aatcgatcgg gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt
      601 atagtttcag tctactatag aactgaccaa gtcagtaacc tagacttagg gcaaggcggc
      661 actgacctag tttgaagaga tttccgaaga gtataaattt taatattttc ttgtgttatt
      721 ccttgacaat tcaatttgga aaatatatga taaagataat gacagcggtg tcattctatc
      781 tattttaaga aaagtaataa tcaattgtta aaaatagtaa aaaaattgga ggttctgatg
      841 aaatattttg ttcctaatga ggtattcagt attcgtaaat taaaggtggg gacttgctcg
      901 gtactattgg caatttcaat tttgggaagc caaggtattt tatcggatga agttgttact
      961 agttcttcac cgatggctac aaaagagtct tctaatgcaa ttactaatga tttagataat
     1021 tcaccaactg ttaatcagaa tcgttctgct gaaatgattg cctctaattc aaccactaat
     1081 ggtttagata attcgttaag tgttaatagt atcagctcta atggtactat tcgttccaat
     1141 tcacaattag acaacagaac agttgaatct acagtaacat ctactaatga aaataagagt
     1201 tataaggaag atgttataag tgacagaatt atcaaaaaag aatttgaaga tactgcttta
     1261 agtgtaaaag attatggtgc agtaggtgat gggattcatg atgatcgaca agcaattcaa
     1321 gatgcaatag atgctgcagc tcaagggcta ggtggaggaa atgtatattt tccggaagga
     1381 acttatttag taaaagaaat tgttttttta aaaagtcata cacacttaga attgaatgag
     1441 aaagctacca ttctaaatgg tataaatatt aagaatcacc cttctattgt ttttatgaca
     1501 ggtttattta cggatgatgg tgcgcaagta gaatggggcc caacagaaga tattagttat
     1561 tctggtggta ctattgatat gaacggtgct ttgaatgaag aaggaactaa agcaaaaaat
     1621 ctaccactta taaattcttc aggtgcattt gctattggga attcaaataa cgtaactata
     1681 aaaaatgtaa cattcaagga tagttatcaa gggcatgcta ttcaaattgc aggttcgaaa
     1741 aatgtattag ttgataattc tcgttttctt gggcaagcct tacccaaaac gataaaggat
     1801 gggcaaatca taagtaagga gagcattcag attgaaccat taactagaaa aggttttcct
     1861 tatgccttga atgatgatgg gaaaaaatct gaaaatgtga ctattcaaaa ttcctatttt
     1921 ggcaaaagtg ataaatctgg ggaattagta acagcaattg gcacacacta tcaaacattg
     1981 tcgacacaga acccctctaa tattaaaatt ttaaataatc attttgataa catgatgtat
     2041 gcaggtgtac gttttacagg attcactgat gtattaatca aaggaaatcg ctttgataag
     2101 aaagttaaag gagagagtgt acattatcga gaaagcggag cagctttagt aaatgcttat
     2161 agctataaaa acactaaaga cctattagat ttaaataaac aggtggttat cgccgaaaat
     2221 atatttaata ttgccgatcc taaaacaaaa gcgatacgag ttgcaaaaga tagtgcagaa
     2281 tatttaggaa aagtatcaga tattactgta acaaaaaatg taattaataa taattctaag
     2341 gaaacagaac aaccaaatat tgaattatta cgagttagtg ataatttagt agtctcagag
     2401 aatagtatat tcggtggtaa agaaggaatt gttattgagg attcaaaggg taaaataacc
     2461 gttttaaata accaatttta taatttatcc ggtaagtata tatcattcat caaatctaat
     2521 ggaaatggga aagaaactgt tatacgtgat agcgatggta atttcaatat tgtaacggag
     2581 aatgggcttt acaaaattgt aacaaataat ttaagtgata aaaacgaaaa agaaaaaaac
     2641 aaagaggaaa aacaatctaa ttcaaataat gtaattgata gtaaccagaa gaacggagag
     2701 tttaactcaa gtaaagataa tatacaaatt aatgacaaga tcgacaataa acaagataat
     2761 aagacagaag aagtgaacca taaaatagtt ggagatggca tagaaactga aaatcatatt
     2821 aataaatcta aagaaatagt agatgtaaaa caaaaattac caaagactgg ttcgaacaag
     2881 attatggaac tattcttaac agtgacagga attagtttac ttttgacact aaaagggttg
     2941 aagtattatg gtaaagataa ataaaatttg ttcgatacaa ggaagttctg tagaaaatga
     3001 ggatactgta ggttcacaaa atcagtattt ttggattatt gatggagcta cagatttata
     3061 taattctaaa gaaggagata ggttattcag tctcagaagt tgtacatatt ttatcagaaa
     3121 gtttgtcggt taattgtaaa gaatcaaaaa ctcttaaaca aatatttgaa actgctttac
     3181 tcgaggttaa agatgaaatc ggtttaaact catatgaatt aacagagtat tatcgcttac
     3241 caacatttgc ttttatattt gctaaacttt cagaaaaaaa gttggagtat atgatgctag
     3301 gtgactgcgt catgttagta aacgaaatgg aaataactga tcatagagta gataacttat
     3361 ttgaaaaagg gaaaaatgag ataaaagact caattggtac aaatagtgtt ttaaataaaa
     3421 aaattatttt acaaaaaatc agaaaattat ctaatcaacc gagtgggtac tggataggtt
     3481 cgttgga
//
LOCUS       DAVKLW010000102         3411 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKLW010000102 DAVKLW010000000
VERSION     DAVKLW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.102"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(284..817)
                     /locus_tag="VW324_002033"
     CDS             complement(284..817)
                     /locus_tag="VW324_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9727581.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(906..1682)
                     /gene="recX"
                     /locus_tag="VW324_002034"
     CDS             complement(906..1682)
                     /gene="recX"
                     /locus_tag="VW324_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9727582.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1720..3075
                     /gene="rlmD"
                     /locus_tag="VW324_002035"
     CDS             1720..3075
                     /gene="rlmD"
                     /locus_tag="VW324_002035"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9727583.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            3137..3208
                     /locus_tag="VW324_002036"
     tRNA            3137..3208
                     /locus_tag="VW324_002036"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3170..3172,aa:Glu,seq:ttc)
BASE COUNT         1098 a          594 c          631 g         1088 t
ORIGIN      
        1 aaatgttttt gatacgaagt tgacctgtat tttttatact aatcattttc gtattttttg
       61 tattaaacga tataagtttg ttgtaaactt actaggaata aagacattaa aaaataatag
      121 tatatctatt tgttttatat attttacgaa ttctgcataa atctctttct agtaatgtgt
      181 tgtaactctg ctataatgga tttattcctt tttgtattta cacaatttat tttatactac
      241 caaaaaaaca aggtcaggat tttgttcctg acctttgaca actttaccga ttctttagtt
      301 ctacatagcg cttgtaccaa atgtttacat aggcttctga gaaaggacca cgtccattgt
      361 taatccaatc aacaagaatt ttgacatgtt cttttaaaat atagtccaag tcatcagaat
      421 aattcatttt gcgtttgtga cgctcgtatt cttcaacgtc caagagacgt ttttccccat
      481 ctgtaaaaat tttaacatcc aaatcgtaat caatatactt cagtgcttct tcatccagat
      541 agtaggggct agccatattg caatagtaag aagttccatt atcacgaatc atggcaatga
      601 tattaaacca atatttcttg tgaaagtaaa caatagccgg ttctcgagtg acccaacgac
      661 gaccatcact ttcggtaaca agtgtatgat cgttgacacc aataatggcg ttttctgttg
      721 tttttagtac catggtgtcc cgccaagttc ggtggagact cccatcatgc ttataacttt
      781 gaattgtaat aaagtcgcct tcttttggaa gcttcataac taaccaactt tctacaattt
      841 ataagtttat catttactat tgtaccataa aattacctaa aatctgtgaa tttcacttgg
      901 aaatattaaa gatattctct aagagcgctt gctatatccg aaaaatcgta gccctttcgt
      961 gctaaaactt gagttaaacg ctgcttcagt tcgtatcctt catactttcg agcatactta
     1021 gtatattgct tatcaagttc cttgaagatg agttcttgag tcgtttcttg gtcaacttgg
     1081 ctatctaagt cgtcaaaggc aattttagca tcagaataag agaagccctt gttagtcaag
     1141 ttctggataa tcttatcttg cagggcacga gctggaagtt ttccctcata ttttttcaat
     1201 agtttattgg ctacacgttg agcaacttca gaaaaatcaa attctttcaa gttctcttct
     1261 atagtagatt ttgaaatccc tttttgagat agtttctgag tcagcacata aggacccttg
     1321 tctcctgaaa gttgattggt attgatgata gcataagcgt actgaccatc attaatccac
     1381 ttatcttctt taagattagc aataacttga gaaacgatgt ttttatcaat atcgtatttt
     1441 ttcagatatt ctctgacttc tttttcagtg cgtgctttaa aggataagtg gtagagagcg
     1501 agattcttac cataagaaaa ttgagcaaag tcttgaatct ctttcaattc ctcttcgctt
     1561 atcaccttat ctctcgataa cataaaacga acaattgtat cttcggtgat atagcatttg
     1621 tcgccattat caagctccat caggtagagt ctttttttct tttcaagttt tgtgattttc
     1681 atagctctat tataactcaa aatgtgataa gataggggta tgaatctgaa agtgaaacaa
     1741 aaaataccat taaaaatcaa gcgcatggga attaacggtg agggaatcgg cttttaccaa
     1801 aaaacattag tctttgtacc aggagctctc aaaggcgaag atatctattg tcagattact
     1861 tctattagac gcaactttgt tgaagcaaaa ttactgaagg tcaacaagaa gtctaaattt
     1921 cgaattgtgc catcttgtac tatttataat gaatgcggag gctgccaaat catgcacctg
     1981 cattatgata agcagctgga gttcaagacg gacttacttc atcaagcgct gaaaaaattt
     2041 gctcctgcag gatatgaaaa ttatgaaatt cgtccaacta ttggaatgca ggaaccaaaa
     2101 tattacagag ctaagttaca atttcagact cgaaaattta aaaatcaggt caaggcgggc
     2161 ttatatgcac aaaactctca ctatttagta gagttgaaag actgcctggt acaagataag
     2221 gaaacccaag tgattgctaa tcgcttagca gaattactta cttatcacca gattccaatc
     2281 acggatgaga gaaaagttct aggtgtccgt actattatgg tccgacgcgc gagaaagacc
     2341 ggacaggttc agattattat tgttacaaac cgccagctta atttaactca attggtaaaa
     2401 gagttggtta aagatttccc agaagttgtg acagtagctg ttaatacaaa tacagctaaa
     2461 accagtgaga tatatggtga aaagacagag attatctggg ggcaagagag tattcaagaa
     2521 ggtgtactca attatgaatt ttcactatcc cctcgagctt tttatcaact aaatcctgag
     2581 caaacagaag tcctctatag cgaagcagta aaagcgctgg atgttgataa agaagaccat
     2641 ttgattgacg cttattgtgg agttggaacg attggatttg cctttgcaaa gaaagttaaa
     2701 acactcagag gtatggatat tattccagaa gctattgaag atgccaagcg aaatgctaaa
     2761 agaatgggat ttgacaatac tcattatgaa gctggaacgg cagaagagat tattcctcgt
     2821 tggtacaagg aaggctaccg agcagatgct ttgattgttg acccaccacg tacaggtctg
     2881 gatgataagt tattagatac tattcttact tatgtaccag aaaaaatggt ttatatttct
     2941 tgtaatgttt cgaccttggc tcgtgatttg gtacgcttag tagaagtcta tgatcttcat
     3001 tatatccagt cggtcgatat gttcccacat acagctcgaa ctgaagctgt tgtaaaatta
     3061 ataaaaaaag tttaaaaaag tagttgacaa agtttgaaaa ggctgtataa tagtaagagt
     3121 tgaaaataac aactcaggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt
     3181 aacacgggtt cgaatcccgt acggactatg gtatgttgcg tcaggaccac ttgatgaaaa
     3241 aaagtttaaa aaaagcttaa aaatcttcaa aaaagtgttg acaagtgaaa gtggctgtga
     3301 tatactaata tagttatcgc ttgagagaag caagtgacaa agacctttga aaactgaaca
     3361 agacgaacca atgtgcaggg cgctacaaca taagttgtag tactgaacaa t
//
LOCUS       DAVKLW010000103         3409 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKLW010000103 DAVKLW010000000
VERSION     DAVKLW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.103"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(1..994)
                     /locus_tag="VW324_002037"
                     /pseudo
     CDS             complement(1..994)
                     /locus_tag="VW324_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1001..1297)
                     /locus_tag="VW324_002038"
     CDS             complement(1001..1297)
                     /locus_tag="VW324_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEU9727584.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(1828..2721)
                     /locus_tag="VW324_002039"
                     /pseudo
     CDS             complement(1828..2721)
                     /locus_tag="VW324_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
BASE COUNT         1257 a          602 c          385 g         1165 t
ORIGIN      
        1 aataacaaat tcaacaaaat actttttata tcattactat aacttgtcac cggaatcaaa
       61 cataacaaca atgaaaattg taagaaagag attaaatgcg tcaacatcat tagttgtgca
      121 ttcttaaaaa agaaattcat tttagaatga ttaagagcag ataattgaag taaagctaga
      181 tctaccccaa ttgaggattt atgattccct attattgcct ccattaatgt aatatatatt
      241 gaggtataaa gaaataatat tggtattttt gtgtcatcaa ttgataacat cgtcatcata
      301 taccaaaata gaatagatag gaaagaagaa tactaatcaa aataaaagaa aaagttaacc
      361 ctttatttaa ccaacaaaaa cgaagcaata taaatagact gctacgaaat cttttttgac
      421 tcattcgact gcttttaaaa tagaaattac tcgcatgatt atctaatatc cccatgacta
      481 aaataaaata attgagtagt atgacataaa ttacaacttg tccttgttct atagacaata
      541 gtcctataga taaaaataag aacaatttac ttataattga aaacaaacta ggtagaatta
      601 aagaaataat ttgaatagct atcaaacaaa gagtaatgga taaataaaaa tatccaataa
      661 tatctagaaa atacaaaata aataaataaa tgaatgattg taaaatttgg aaagaataat
      721 atttcttgac aatggcaaga agatagagaa aaatatagtt tttccttata tagaaagtat
      781 ttattttttt actttggata ggcagtttca tattataacg attcaaagta gcgtaatcta
      841 ctaccaaaaa aagtaaaaat aatttccctt gaaaaggaag atagctttta aaatagattg
      901 acaaacttat gataacaaaa aacaaaaggg ttatcccaat aaaattaaga taacctaaaa
      961 tactttcaca ttctttttta gaaagtattt tcatctagtt ctaccataga gctgctttag
     1021 cagcaccttt ctttttcaat atataaagaa ctgttgctgc aatacccgaa ccaatagaac
     1081 ctactcctgt gattgcacct acaattgaaa ttactgctgt aactgtacta taagcactaa
     1141 tcaaattgac tacagtagca gcagcttttg tcgaaattcc taatgtccca gccaacatag
     1201 gaaaagaaac aacacctaag cttaaccaac taatgactgc taacattgct gaaacaataa
     1261 gaaatttatt atgaaattcc attcttttag atttcatttt agtgtactcc ttttgtatat
     1321 tatgtatgtt ttattgagag ttaattcgca catgttgtat tcctaactct cttcaaatgt
     1381 tcgtctatat tttatactac ttttattaac atatctataa taaataaatt actactacat
     1441 tttcctagat tatcaaatta cttttatatc actaagactc aataaaagat aatgtctatt
     1501 taacttttat acgattacta ccttcttttt agtttacact tcctacatca taaataaaca
     1561 tactcaaccc tcacaatctc caaataactc ttctatatca ttgaaaattg agtttcatcc
     1621 tctatactaa tgtaggacat tcctttaatc caatataaga ataccttgtt ccattaatct
     1681 ctacccgata ttatagatag aaccaaattc ctatacttaa aaggacaaat aacgttccaa
     1741 ccataataaa cctcctttct ttttgagaac gttttcttta taggtttagt atagcaaaaa
     1801 catgaaaaaa acaacatgac ttagaagtca ggttgttggg aaatctatct gtttttctgc
     1861 taaaattctc tgaactaata cctgatcgtc tagcaattct gctagagaaa tcgccttttc
     1921 atagaaactt tctgcctcta aaatattctt gtctacaaat aaagcatatt ttgcctttag
     1981 caaaaataga tgaggtcggt aaaaaacatg tcttgtttta gcaataattt tctcaaatgt
     2041 ttgaatatat atcagactat cttctgtctg aatttggata aataggctca gaagaacttt
     2101 ttccatttga actaggctag ccttatcttc atattctgaa catagcaata ctttttgagc
     2161 taactcctca aaatgttctt tatcttctaa tcccatagca caacaaaaga aatacaattc
     2221 aataatcaac aaatcattta cagaaaaata tttctttttt aataactgct ggaaatattc
     2281 ttccactaac cctaaaccgt aggtcacatc tgaactacca taaatatcaa aacgagcttg
     2341 tgtcacatcc acaattaact gctcttcctc aggcaaattc tcataatagt tttcatatat
     2401 ttcctcaagt atagcttctt ttcattcgat tcttccctta tcagcgtagg tttgagaatg
     2461 aatcagctca tctttgagtt ttaggaatcc ttttggaatc tctataacct taacatccac
     2521 aatattctgt aatgatactc caagtttatc cgcaatcaat aataatttag gaagtgttgc
     2581 taaagcttgc ccattttcaa tacgtgctag ctgacgaatc gtcaactctg attcatcacc
     2641 acaaatatct atctgagttt ttccacaaga tcgcctaata gttttgattt tataaccaat
     2701 ttcacgtttc aactcatcca tagtaatact ccttaagttg attacaaaga tttctttttc
     2761 ttcctaattc ttttgtcaat catatataaa acgataagcg aaaacagcgg tagaacaata
     2821 taactctttt cactcattat cagctgaaat cccagggttg ctagtatctt aaagatgaaa
     2881 atattcatca aaaatagaaa aacaaatact aaaaaatatt ttaaaacctt cttcatttct
     2941 tttcttcaac tttcctaatt tctacaccta ttttatcgaa aatatatata aaactccaaa
     3001 taaacacttc aactgcactt tttttggcaa aaaatagaca gaacaaacat cctacctact
     3061 aaaaatatga tatgaaagga aaatgatttt ttactgacac cctaggcaag cttgggacaa
     3121 ataaatcatt gatagcaaca tttacgacat cctatttaaa gttccttcaa tttctaatgt
     3181 ccccgctatt tctaagccac ctctggtatg atctattcct agtcagaata gataacaccg
     3241 ttctaaatta cgattcatag ttactccttt tttgactcgt tactataaga ctcctattta
     3301 catgcgcaaa ataattttga ttcagattca tcatttatag ttttgattat tttttaacag
     3361 atttttctgt tttcttctcc gaagacttta aaaatttttt tctaattaa
//
LOCUS       DAVKLW010000104         3392 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKLW010000104 DAVKLW010000000
VERSION     DAVKLW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.104"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            225..1511
                     /locus_tag="VW324_002040"
     CDS             225..1511
                     /locus_tag="VW324_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9727585.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKGRKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1526..1837
                     /locus_tag="VW324_002041"
     CDS             1526..1837
                     /locus_tag="VW324_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727586.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2083..2811
                     /locus_tag="VW324_002042"
                     /pseudo
     CDS             2083..2811
                     /locus_tag="VW324_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            3193..>3392
                     /locus_tag="VW324_002043"
     CDS             3193..>3392
                     /locus_tag="VW324_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9727587.1"
                     /translation="MEVINVSKHYGHSIILKDINFALNKGEIVGLVGRNGVGKSTLMK
                     ILVQNNQPTSGNIISSDNVGYL"
BASE COUNT         1081 a          561 c          727 g         1023 t
ORIGIN      
        1 atgtattttt acgagtcagt atctttggat actggttttt acttttctag actttttgac
       61 tatttgttaa aactgggata attttcgact gtttaacagt tattatgcaa agtctaaaag
      121 attagaattg tcaaaacaat ccgtctaggc ttgattttat cctttattta ctataaaatg
      181 agaaggaaaa atgtcaaact tttatattgc aaataggaga aatcatgaca aaaacattaa
      241 aacgtcctga ggttttatca cctgcaggga ctttagagaa gctaaaggta gctgttcagt
      301 atggagcaga tgctgtcttt atcggtggtc aggcctatgg tcttcgtagc cgtgcgggaa
      361 actttacttt cgaacagatg gaagaaggcg tgcagtttgc ggccaagtat ggtgccaagg
      421 tctatgtagc ggctaatatg gttatgcacg aaggaaatga agctggtgct ggtgagtggt
      481 tccgtaaact gcgtgatatc gggattgcag cagttatcgt atctgaccca gccttgatta
      541 tgattgcagc gactgaagca ccaggccttg aaatccacct ttctacccaa gccagtgcca
      601 ctaactatga aacccttgag ttctggaaag agctaggctt gactcgtgtc gttttagcgc
      661 gtgaggtttc aatggaagaa ttagctgaga tccgcaaacg tacagatgtt gaaattgaag
      721 cctttgtcca tggagctatg tgtatttcat actctggacg ttgtactctt tcaaaccaca
      781 tgagtatgcg tgatgccaac cgtggtggat gttctcagtc atgccgttgg aaatacgacc
      841 tttacgatat gccatttggg aaaggacgta agagtttgca gggtgagatt ccagaagaat
      901 tttcaatgtc agccgttgac atgtctatga ttgaccacat tccagatatg attgaaaatg
      961 gtgtggacag tctaaaaatc gaaggacgta tgaagtctat tcactacgta tcaacagtaa
     1021 ccaactgcta caaggcggct gtggatgcct atcttgaaag tcctgaaaag tttgaagcta
     1081 tcaaacaaga cttagtggac gagatgtgga aggttgccca acgtgaattg gcaacaggtt
     1141 tctactacgg tataccatct gaaaatgagc agttgtttgg tgctcgccgt aaaatccctg
     1201 agtacaagtt tgtcgctgaa gtggtttctt atgatgatgc gacacaaaca gcaaccattc
     1261 gtcaacgaaa cgtcattaac gaaggggacc aagttgagtt ttatggtcca ggtttccgtc
     1321 attttgaaac ctatattgaa gatttgcatg atgccaaagg caataaaatc gaccgcgctc
     1381 caaatccaat ggaactattg actattaaag tcccacaacc cgttcaagct ggagacatgg
     1441 ttcgtgcatt aaaagaagga ctcatcaatt tttataagga agatggaacc agcgtcacag
     1501 ttcgagctta agaaaggaaa aggaaatgat agaggcacag ggtttcttag tggataagca
     1561 aacaagatgc attcattacc atagcaagct ggatattatt gctttacaat gctatgattg
     1621 taaaaagtat tatgcttgtt atcggtgtca tgattcatta gaaaatcacc cttttgagcc
     1681 gtatccctta tctttgatac aggataagcc tattttatgt ggtgtttgtc taaaactatt
     1741 aacatataag caatataaag aaagtttaag ttgccccttt tgtttttctc gctttaatcc
     1801 aggttgccaa aatcataagg aacgctattt taaatagcaa atcatctagt tttgaagtag
     1861 gagaaaactc aattccaaga gaaaatgaag taaatcttcc cacaataaaa cgcataatat
     1921 caagattgtt caatacctga tactatgcgt ttttaagatt ttaaagactt ttttccttta
     1981 tctggtattt tgactacttg ttaaaactgg gttaattttc aactgtttaa cagttattat
     2041 gcaaagtcta aaagattaga attgtcaaaa caatccgtct agagtatgcg tgatgccaac
     2101 cgtggtggat gttctcagtc atgccgttgg aagtacgacc tttacgatat gccatttggg
     2161 aaagaacgta agagtttgca gggtgagatt ccagaagaat tttcaatgtc agccgttgac
     2221 atgtctatga ttgaccacat tccagatatg attgaaaatg gtgtggacag tctaaaaatc
     2281 gaaggacgta tggagtctat tcactatgta ttaacagtaa ccatctgcta caaggcggct
     2341 gtggatgcct atcttgaaag tcctgaaaag tttgaagcta tcaaacaaga cttggtggac
     2401 gagatgtgga aggttgccca acgtgaactg gctacaggat tttactatgg tacaccatct
     2461 gaaaatgagc agttgtttgg tgctcgtcgt aaaattcctg agtacaagtt tgtcgctgaa
     2521 gtggtttctt atgatgatgc ggcacaaaca gcaactattc gtcaacgaaa tgtcattaac
     2581 gaaggggacc aagttgagtt ttatggtcca ggtttccgtc attttgaaac ctatattgaa
     2641 gatttgcatg atgccaaagg caataaaatc gaccgcgctc caaatccaat ggaactattg
     2701 actattaaag tcccacaacc tgttcaatca ggagacatgg ttcgagctct taaagagggg
     2761 cttatcaatc tttataagga agatggaacc agcgtcacag ttcgtgctta atgtagttgt
     2821 ttagttttaa aaaattatgc aaagctccat atacaacatt taaacgagat taaagaatgg
     2881 cgaaatccct tgatgcgcaa gagattagct gtcctttttt attttttaag tgataaagtc
     2941 ggagcttagg tatcaaagcc tatcaaatta aacaaagaag cgatgtctta gatattttga
     3001 aaaaaattaa taagcagaaa actctctatt attttgttgt agagagtttt ttattaataa
     3061 aattttacaa aatgacattt atatattgca ttaagttaga tatatgatat aatgttgtta
     3121 aaaagaggcg caacttttta aactcaataa gaatcaaaga gcaaaccaag aatattaatg
     3181 gaggaataaa aaatggaagt tataaatgta agtaagcatt atggtcattc aatcattctc
     3241 aaagatataa attttgcact taacaagggt gaaattgttg gtctagtagg gagaaatgga
     3301 gttggtaaga gtacgttgat gaaaattctt gttcagaata atcaaccgac ttcagggaat
     3361 attataagca gtgataatgt tgggtattta at
//
LOCUS       DAVKLW010000105         3317 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKLW010000105 DAVKLW010000000
VERSION     DAVKLW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.105"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            238..969
                     /locus_tag="VW324_002044"
     CDS             238..969
                     /locus_tag="VW324_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727588.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            984..2135
                     /locus_tag="VW324_002045"
     CDS             984..2135
                     /locus_tag="VW324_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9727589.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRASNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSICLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(2187..2846)
                     /locus_tag="VW324_002046"
     CDS             complement(2187..2846)
                     /locus_tag="VW324_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9727590.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            2916..>3317
                     /locus_tag="VW324_002047"
     CDS             2916..>3317
                     /locus_tag="VW324_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9727591.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQM"
BASE COUNT         1215 a          482 c          671 g          949 t
ORIGIN      
        1 aaaggtcagt tttatagtga attgaatctg gaatattaca ctacggttcc taaaatattt
       61 tcagaaagcg atttgaattt cccaataaat ttgttcagtt tttaattgat tttactatag
      121 aagatataat tttctaaatt actctgacac agaactttaa gaaaaagata attattgtag
      181 taatttcttt aagaaaatga tataattcat taaaaagtat ttggaggagg aagaattatg
      241 ttgaatcagg atctctttga ttcgcttgag gcacaaaaaa ttgtagatac tttgatgaaa
      301 gggcaaaaag attatgtaga tgaacgtcta gaaaaaagag agacaatgat agtgtcgaat
      361 ggttatgcat ggacgcgacc taatcatatt gatactgcat ttgcatcagc agatttgttt
      421 gagtataaat tacaattagc aggacagact tggggatatt tagaatttga aacaaataca
      481 gaaaaatatg ggaaagtatt gttaattata aagggtaaga agcgacttac gaaccaattt
      541 cctttggtac aaaaaaataa gagtggctac ttatttgaat acgctcagat gaatacactt
      601 tatcttaatc aacattcttc ctacaaaaat gatgaagata gtcattcctt tccaattcag
      661 atggagttag tttctgatga aatgattcaa gaaattgaac aagctactaa aaattcgaat
      721 atcgaaaaat ttatgatttt aacttatgag gcggactcag aaaacaatat tatatctgta
      781 gatgttgtta tgcctgatgc acgaactggt cagctacact tgattcaaga tttgtctgag
      841 tatattcaat caagttcgta ccatttcgaa gaagctaaat atcaagatat tcctaatttt
      901 tcagaattat cggaaacaga agattttgaa attattccaa gaatagaaaa acaagaaggt
      961 caaaagtagt aaggagtata taaatgttta atggtcgggt attgaaagaa ttacggctgt
     1021 taaatggttt aagtagagca gaattagctc agagaattaa tttaacggaa caagccattt
     1081 ggcagtttga gtccaacgaa acgaaaccta aattatcaac caaaatgcat ttggccaacc
     1141 aatttcatgt tgatttaact tattttgaac aggaagaaga gagcattcga tttgattctt
     1201 ctgtaattgc ctttagaaat gcagacctag caacacggaa aacaatagat attcaaacta
     1261 tgtatttaca taaggtagat agtttgattg attattttga aagttttgta attataccta
     1321 atattataat tcatgaccta agtaatgtag tgagtgaatc ttatcataag ggagaatcca
     1381 ttgaggaatt ggctctttat gccagggaaa aattaggtat ttcaaaagat aatcatgatt
     1441 tgctttataa attagaacgt tcaggtatct atatcgtgga acgattaatt aatggccaag
     1501 ctgatgctta tagcgcatgg tcaaaattgg gaagacctta tattgtgttg ggaacgaata
     1561 aatcatctgt acgtcgaaat tttgacttag ctcatgagct aggacatatt cttttacata
     1621 aatataagga tatgaatgaa gatggcgatc gtttggagca agaagcaaat tattttgcat
     1681 catgtttttt attgccaaaa gaagagtttt tagtcaaatt tgaagagagg gttggcaagc
     1741 gtgccagcaa tcctgatagt tatattttat tgaagtcgga tttgaatgtt tcgatacagg
     1801 ctttagagta tcgagctttt aagttaggat tattgactcc aaagcaacat tcttactttt
     1861 atcgtcaaat tgcgcaaaaa ggttacaaaa tgattgaacc tttggatgat caaatttttg
     1921 ttaaaaaacc aagcaaagta aagagtattc tggacgtcgt tttgagtaat catctagtca
     1981 gtctagcgac tataatgtct aaacaaagta tttgtttaca gtttataagc gaaatatttt
     2041 cagtcgaaat gaaatttttt gatcagtatc aagaagatag aagaacagat cgatttgata
     2101 acatcatccc tttgtacaaa agaaataatt tataatttaa ctgtgaaata atttcatgaa
     2161 ggacatatgg cagaaattgg attttatcag acaagcagta gcttaccaaa aataatcaaa
     2221 caagtagcaa gtaagaggaa aggcacgaaa ggaagtcttt cctttttctt ttgcaggata
     2281 aaggccagga tacccgtcgc agaagcaaac tgaatcaaga tcagtaactc cgttacgcta
     2341 aagacgagag cacaagaagc taaaaagagg aaatctcctg cgcccatgcg gatatcgata
     2401 aaatgagcca aaattccaag agcaaggaag gagaccatga ccagattcca gccagaggaa
     2461 gctattagga ttaggtggaa agtcatccag accagtaagg gatattcctg atggtgaaag
     2521 tcgtagatac ccaaggtcaa accagcggtg attaggacga cttgccccaa ggaaagcaat
     2581 tcccaagagt aaagcagaaa gaggagtcct aagactaatt caaagagggc ataccagaca
     2641 ggatagcgaa ctttgcagta gcgacagcga aagcgattga agacctgtga gagaatcgga
     2701 atcaaatcta agggacgcaa gcgagtctga caggaatcgc agtgactggc tgaactgata
     2761 atggattgct ctggaaaacg gtcaatgacc aaaccaagaa aggaagcgag aatgctcccg
     2821 acgagaaaaa aataaaaatc aatcatactt atctattcgt aaaaaatggg agaaatagta
     2881 taatggagaa acatagataa gatggtgagg aaaagatgac aattcgtttt gaagaaaagg
     2941 tgagtataga aaacgctcaa tttgtatgcc agtggtccaa ctccctaggc aaatcctttc
     3001 aagaacaatg gatgggacca aggattcctt ttctactgac ccttcaagcc ttggaaggag
     3061 tcttttcaat ctttgatgaa caagagtttg tgggacttat ccagaaaatc aggctagaag
     3121 acagcaatct tcatatcggg agatttttta tcaaccccca gaaacaggag caagacttag
     3181 gtagccaggc tttaaggaaa tttgttagtt tggcctttga aaatgaagat atagatagta
     3241 tttctctaaa tgtcttcgag gcaaatcaaa gagctcagaa tctttaccaa aaagaaggat
     3301 ttgaaatcgt tcaaatg
//
LOCUS       DAVKLW010000106         3194 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKLW010000106 DAVKLW010000000
VERSION     DAVKLW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.106"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..175
                     /locus_tag="VW324_002048"
     CDS             <1..175
                     /locus_tag="VW324_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9727592.1"
                     /translation="NRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDTSSSDVTL
                     ITGKNGTGKSTLL"
     gene            complement(541..1851)
                     /locus_tag="VW324_002049"
     CDS             complement(541..1851)
                     /locus_tag="VW324_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9727593.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2088..2960
                     /locus_tag="VW324_002050"
     CDS             2088..2960
                     /locus_tag="VW324_002050"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9727594.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3091..>3194
                     /locus_tag="VW324_002051"
     CDS             3091..>3194
                     /locus_tag="VW324_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9727595.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNT"
BASE COUNT          986 a          538 c          717 g          953 t
ORIGIN      
        1 aaacagaact gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat
       61 ttcgtatagg gtggatcatc gtaaattgtt tgataatatt tcttttgata cttcgagttc
      121 agacgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat
      181 tgaaactaga atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc
      241 tgatctattt gtcctgttct tatttcattt cactatatct caaattgagt atgacgaagt
      301 gcgctcccat gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga
      361 taaagactat tgggatgaat ttttaaagtt gaactaatca tttttacagg atgagattta
      421 cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt
      481 tccacttaat tttgtgtcta ctttattata ccaagtccac tcttaagtta gataataaat
      541 ctaacttaag gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct
      601 gatggggaca cccagaataa tttgcttttt gaaggcaagg ccacgttcct ctatattggg
      661 aagtgagagt tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc
      721 gccaataacg gtggctgttg tgagtaagtg aatatcaatc tgaggatttt gagcactgat
      781 gatagcaatg atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga
      841 catcactccg gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggttgttgt
      901 catgaggtgc cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct
      961 catgccgcag agcatgataa ttgtagccca gggaacctta gctaaaatgg cttcttgctt
     1021 ccctaatttg agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa
     1081 tgttttttga taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa
     1141 aagccaaacc aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct
     1201 gaggagtggt ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtgact
     1261 gtaacaggat aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg
     1321 attgccaagt gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac
     1381 aatgcctgag ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg
     1441 agctccaatc aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca
     1501 aacggccata gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta
     1561 taaaatcatg tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt gttgagccaa
     1621 aacatcaaga gtaccgttag ttgttgcaac gttataaaag agagagacgc taaaaatggt
     1681 aaaaaagagt gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt
     1741 ggttgcgatg aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta
     1801 accaattcca atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc
     1861 ctttcagtta gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc
     1921 ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac
     1981 acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagta
     2041 attgcctcta gtgcttacaa gtgatattaa aaatagagga cttagtgatg tcaatcattt
     2101 caactgattt aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg
     2161 ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt
     2221 cgctttttgc caaggaagct ggtgttttag cgggactaac cgtttttcaa agggttttta
     2281 ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt
     2341 tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg
     2401 aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt
     2461 atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta
     2521 atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat catcgcttta
     2581 atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt agtgttcaga
     2641 aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg
     2701 aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata
     2761 atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg
     2821 aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt
     2881 acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc atgaagggtt
     2941 taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc
     3001 tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatatagtaa aatgaaccaa
     3061 aaatagtaca caatgtggta taatcttctt atggcatatt caatagattt tcgtaaaaaa
     3121 gttctctctt attgtgagcg aacaggtagt ataacagaag catcacacgt tttccaaatc
     3181 tcacgtaata ccat
//
LOCUS       DAVKLW010000107         3053 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKLW010000107 DAVKLW010000000
VERSION     DAVKLW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.107"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..1001
                     /locus_tag="VW324_002052"
     CDS             <1..1001
                     /locus_tag="VW324_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9727596.1"
                     /translation="ILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDK
                     KPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFD
                     DLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKID
                     SKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTS
                     KDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEAL
                     QKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            991..1998
                     /locus_tag="VW324_002053"
     CDS             991..1998
                     /locus_tag="VW324_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9727597.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            1998..3005
                     /locus_tag="VW324_002054"
     CDS             1998..3005
                     /locus_tag="VW324_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9727598.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
BASE COUNT         1041 a          435 c          534 g         1043 t
ORIGIN      
        1 ctattttagc tatgtgtata gtttttagcg cttgttcttc taattctgtt aaaaatgaag
       61 aaaatacttc taaagagcat gcgcctgata aaatagtttt agatcatgct ttcggtcaaa
      121 ctatattaga taaaaaacct gaaagagttg caactattgc ttggggaaat catgatgtag
      181 cattagcttt aggaatagtt cctgttggat tttcaaaagc aaattacggt gtaagtgctg
      241 ataaaggagt tttaccatgg acagaagaaa aaatcaaaga actaaatggt aaagctaacc
      301 tatttgacga tttggatgga cttaactttg aagcaatatc aaattctaaa ccagatgtta
      361 tcttagcagg ttattctggt ataactaaag aagattatga cactctatca aaaattgctc
      421 ctgtagcagc atacaaatct aaaccttggc aaactttatg gagagatatg attaaaattg
      481 attcaaaagc cttaggtatg gaaaaagaag gtgatgagtt aatcaaaaat actgaagctc
      541 gtatatccaa agaattagaa aaacatccag aaatcaaagg aaaaatcaaa ggaaaaaaag
      601 tattatttac tatgattaat gctgcagata catcaaaatt ctggatttat actagcaaag
      661 atccaagagc aaattattta acagatttag gtctagtttt ccctgaatca ttaaaagaat
      721 ttgagagtga agatagtttt gcaaaggaaa tttctgcaga agaagcaaat aagataaatg
      781 atgctgatgt aatcataact tatggtgatg ataaaactct tgaagcttta caaaaagatc
      841 ctcttttagg taaaataaat gcaattaaaa atggtgccgt tgctgtaatt ccagataata
      901 caccgttagc agcctcatgc actccaacac cactttcaat aaactatact attgaagaat
      961 atctaaatct tttaggaaat gcatgcaaaa atgcgaaata aaaaacaaat aaacctaggc
     1021 ataatttttg taatctgcct aggtcttctt attacaatat ttttgtcatt aaagcttgga
     1081 acaaaagaaa ttaatatcag agatttttta gcagcttttg gaatgggtaa tacaaatgat
     1141 gattttatta aatcaattat atataaaaga atacctagaa ctatttttgc aattttagca
     1201 ggttctagtc ttgccataag cggtgtattg atgcaatcag ttactagaaa cccaatagct
     1261 gatccaggta tacttggtat aaacacagga gcaagtctta gtgtagtaat tggtctttct
     1321 tttttaggaa tttcatcaag cataagccat ataagttttg caatcattgg tggcttagta
     1381 agtgcaattt ttgtatacgc gattgctgta agcggaaaag caggccttac ccctataaaa
     1441 cttgccttat caggaacttg tgttagtatg gctttaagca gttttgtaag ttttttaatt
     1501 ttaccgaata ataacgtctt agacaaattt agattttggc aaataggtag ccttggagca
     1561 gctacattat cttctatatc tacactacta ccttttataa ttttaggtca cttgatagct
     1621 atatttattt catcagattt aaacgcttta gctatgggtg atgaaatggc tgttggtctt
     1681 ggagttaatg ttaataggat aagatcactt gcaataattg caagtgtgct tttatgttca
     1741 agtattactg caattggtgg acctattggc ttcgtaggtc ttatagttcc tcacttttgt
     1801 ggcttattta taagcaaaga tatacgcaca atgaccattt cttcatcttt tataggtgca
     1861 gagctcttgc ttatatgtga tataatcggc cgtatgttag gtaaaccagg tgaaattgaa
     1921 gtagggataa ttactgcaat aatcgggggt ccagtactta tttatgtaac tatgaaaaat
     1981 agaggggtta ataactaatg caaaatttaa ttataggtat tcaaaaaaga aaaaatagaa
     2041 taacactatt ctcctcacta tttttattaa taataatcag tctatcattt ttcattttac
     2101 ttatcggaga tgaaagttat tctttttcaa ctttgattaa agtcttaaat agtgaaactg
     2161 ttcctggagc tagtttttcg attatggaaa ttagattacc aaaattatta gcaggaatta
     2221 tagctggctg gtcttttgga ttggcaggat ttatctttca aactatgtta agaaatcctc
     2281 ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca
     2341 tattggtatt aaaaacaaat agtttaacta ctggaattat ttcaataact tgtggactaa
     2401 catcatcttt aatattattt ttactagcta aaaaagatgg tttttcagca gcaagactga
     2461 taatattagg tattggtttt caagctgtca caagagcagg cacctcattt ttattgttga
     2521 aagtagcaag atatgaatta caagaagtta tgagatggct cagtggctct ttatctttta
     2581 caaagttaga tgacatacct cttgttctaa tagtaagtat tattgctact atattagttt
     2641 tattttttaa taaaagacta gaaattattg aacttggtga agaaatagca atcggacttg
     2701 gagcaaatcc cgagctttca aggcttgttt taattttttg cgctgtatct ttaactgctt
     2761 tttctacttc aattacagga ccaatagctt gtatatcttt tttagctggt cccatagcct
     2821 taaatattgg taagaaaaga agcccaatat tagctggatt ggttggaatt ttactagttt
     2881 tgttatcaga catattctct caaaatattt taccagctag atatccagta ggtgttgtaa
     2941 ctggcttgtt aggttcacca tacttaatat acttactaat aaaaatgaac aggagaaata
     3001 tataatgaaa ggtctgtggt caaatgatct tacctgcggt tatgatgaaa aaa
//
LOCUS       DAVKLW010000108         2389 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKLW010000108 DAVKLW010000000
VERSION     DAVKLW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.108"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="VW324_002055"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="VW324_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9727599.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1449..2389)
                     /locus_tag="VW324_002056"
                     /pseudo
     CDS             complement(1449..2389)
                     /locus_tag="VW324_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          744 a          464 c          499 g          682 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacacg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaatccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggctct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat ttcgtccttt cttttttgat gttcagggcg
     1501 atgaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata
     1561 agtttgatga gattattggt cgcttccaat ttggcgtttg aatagggtag ttgaagggcg
     1621 ttgacgattt tctttttgtc ctttagaaag gttttaaaga cagtctgaaa aagaggatga
     1681 acctgcttaa gattgtcctc aatgagtccg aaaaatttct ccggttcctt attctgaaag
     1741 tgaaacagca agagttgata gagctgatag tggtgtttca agtcttccga ataactcaaa
     1801 agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaatcgc
     1861 ttatcactca gtttacgact atcctgttgt atgagcttcc agtagcgctt gatagccttg
     1921 tattcatgag actttcgatg aaactgattc atgatttgga cacgcacacg actcatagta
     1981 cggctaagat gttgtacaat gtgaaagcga tccaacacga ttttagcatt cgggagtgaa
     2041 acagtctggg agactgtttc agcctgagcc tagaaatttg aaagcgaagc tgtttagcta
     2101 agtcatagta aggactaaac atatccatag taataatttt gacgcgacat cggacggctc
     2161 tatcatattt aagaaagtga tttcgaatga tagcttgtgt tcttccctca agaacagtga
     2221 tgatattgag attgttaaaa tcttgcgcaa tgaagctcat ctttcccttt gtaaaagcat
     2281 actcatccca agacataatc tcaggaagac aagaaaaatc atgtttaaag tgaaagtcat
     2341 tgagcttacg aataacagtt gaagttgaga tggaaagctg atgggcaat
//
LOCUS       DAVKLW010000109         2375 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKLW010000109 DAVKLW010000000
VERSION     DAVKLW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.109"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..415)
                     /locus_tag="VW324_002057"
     CDS             complement(<1..415)
                     /locus_tag="VW324_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU9727600.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGKEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
     gene            537..1283
                     /locus_tag="VW324_002058"
     CDS             537..1283
                     /locus_tag="VW324_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9727601.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1296..2276
                     /locus_tag="VW324_002059"
     CDS             1296..2276
                     /locus_tag="VW324_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9727602.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          852 a          287 c          452 g          784 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttctttaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccgggc
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
      481 aggttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg tgtaaaatga
      541 aaagattgga acaaattatt aaattagtat cagaatatga aaagatcgac gttaatacat
      601 tatcggaaaa attaaatgta tcgaaagtaa cgattagaaa agatttagat aaattagagt
      661 caaaaggttt attacacaga gagcatggat atgctgtatt aaatagtgga gatgacttaa
      721 atgtacgttt gtcaattaat tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat
      781 tggtgtcaga taatgaaaca ataatgatag aatctggatc gacctgtgct ttacttgctg
      841 aggaaatttg caagcaaaaa agaaatgtta cgattgtaac aaattcattt tttatagcaa
      901 attttgtgag agcttatgat tcatgtcgta ttattgttct tggtggagag tttcagaagg
      961 attcacaggt tactgtggga cctttattaa aagaaatgat acagactttt catgtgcatc
     1021 aagcttttgt tgggacagat ggttacgata aagagatggg ctttacagga aaagatttaa
     1081 tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga taaagtcatt gtactaactg
     1141 actcaagtaa atttgataaa aggggtacag taagaagatt tgctttaagt caagtctatg
     1201 aagtaataac agacgaaaaa ctttctaaac aaaatatagc tacattagaa aatgctggga
     1261 taatggttaa ggtagtttcg taagaggtta agtgtatgaa tcaagatagg aataaactgc
     1321 tttctaaaat tgcttatctg tattatattg aaaacttaaa tcagtcacaa atagcagcaa
     1381 aattaggaat ttatagaacc tctattagta gaatgttaac agaagcaagg aatgcaggaa
     1441 ttgttaaaat tgaaatagag aattttgata ccaatatgtt taagttggaa aattatgtaa
     1501 aagaaaaata tggtctggaa agtttagaaa ttattccaaa tgaatttgat gataatccaa
     1561 caattttatc tgaaagaatt tctcaagttg cagcaggtgt ccttaggaat ctaattgatg
     1621 ataatatgaa aattggcttt tcttggggga aaagtttaag taatttagta gatttaattc
     1681 acagtaaaag tgtccgaaat gttcacttct atcctctagc aggtggtcct agtcacatac
     1741 acgctaaata ccatgtgaat acactgattt atgaaatgtc tagaaaattt catggagagt
     1801 gtacatttat gaatgcaacg attgtgcaag aaaataaatt gttagcagat ggtattttgc
     1861 aatcaagata ttttgaaaat ttgaaaaata gttggaaaga tttagatata gctgtagtcg
     1921 gaattggtga ttttagcaat aaaggaaagc atcaatggtt agacatgctt acagaggatg
     1981 actttaaaga attgaccaaa gttaaaactg taggagaaat ttgttgtcga ttttttgatt
     2041 caaaaggtaa agaagtctat gaaaacttac aggaaagaac gatagcaatc tctttggaag
     2101 atttaaaaaa tattcctcaa agtttagctg ttgcttacgg tgatacgaaa gtatcttcga
     2161 ttctttctgt cttgcgtgct aatttagtaa atcatttgat tacagacaaa aatacaattt
     2221 taaaagtttt ggaagaagat ggggatttga cttttagaga gattctaggt gagtgaaaat
     2281 gatagactga ttcagtttat cgtttttctt tttagttgat tgcacatttg tgcttatata
     2341 aacaaaaata gtttatctgt tgtttttgga ttgac
//
LOCUS       DAVKLW010000110         2233 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKLW010000110 DAVKLW010000000
VERSION     DAVKLW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.110"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..357)
                     /locus_tag="VW324_002060"
     CDS             complement(<1..357)
                     /locus_tag="VW324_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEU9727603.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFF"
     gene            complement(475..888)
                     /locus_tag="VW324_002061"
     CDS             complement(475..888)
                     /locus_tag="VW324_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9727604.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(885..1121)
                     /locus_tag="VW324_002062"
     CDS             complement(885..1121)
                     /locus_tag="VW324_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9727605.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(1181..1602)
                     /locus_tag="VW324_002063"
                     /pseudo
     CDS             complement(1181..1602)
                     /locus_tag="VW324_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(1625..2158)
                     /locus_tag="VW324_002064"
     CDS             complement(1625..2158)
                     /locus_tag="VW324_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9727606.1"
                     /translation="MKETYTDEHLEIMRDNCENLRDLAIIDLLASTGMRVGELVQLNR
                     SDIDFENRECVVFGKGKKERPVYFDARTKIHLRNYLNDRKDSHPALFVTLVGKAQRLG
                     IAGVEIRLRKLGDKLGIQKVHPHKFRRTLATKAIDKGMPIEQVQKLLGHSKIDTTLAY
                     AMVNQNNVKHSHQKFIS"
BASE COUNT          655 a          430 c          321 g          827 t
ORIGIN      
        1 aaaaaaagaa gacaatatac gcctaatatt gtctaaattt gccttactac aatccttttc
       61 agactggtaa tttgctaaat aactccttaa atcatctgtt gtgagttgtg tcacagactc
      121 tttaatagca ttaaacaagt tttcaatcgt cctctggtaa taacgaattg tttttgagct
      181 acagccctct acctgcttgg cggtaataaa tagttgcaac aggttattat tcgaatactt
      241 ctgctgcttt tttccgtcta ctagatgatg ttctaaactc tctctcaacg ctagtaattg
      301 ttcattattt aaaagaggta acatttcttg catgataatt gtaacttttg attccataat
      361 ttctcacttt cttattcatc aataaaaaga gacaatatca gtttctgcat tacagaattc
      421 taatattgtc tgttcattta ttttcttgtg ctcattcaaa tccagcatac taggttactt
      481 tttgaccttt tctctaacag aatgttcaga aacccatttg gagtagcttg tcattttttc
      541 atcagttaaa gcttctactg cgatggttac acacattttt actgcttctt ctaccgttac
      601 agaaaaacga tagccgttta attgtaaaaa tttgaccaaa acgaagaaag cagttctttt
      661 attagcatta gcaaaaacat gcttctttat caattggaca aatagtatcg ttgctttatc
      721 aaaaattgtt ggataaagag acttcccaaa gacaaattgt tctggtaagt tcacaatcat
      781 atttaaggca gaagaactaa ctgtttgaat tttctcattt ggagaatacc gttgaattgc
      841 taaagcattt attttttcaa tttgcttttc tgtcagatag attgtcattt ttccaccaaa
      901 gctttgaaaa catcgtcgta ttcgtcaaat attttatcta aatttaagtc aaaggcatca
      961 tcagatacat aagaaacctg ctgccccaat tcttcaggag taaagataat ctcaccactt
     1021 ggtaacaatt tcgcttcgta tttaacacca cttggaatat taaattcact tggaatcgta
     1081 atggtgattg aatttccttg ttttcttgtt tttactacca tctgatttcc tcctttgaag
     1141 tattataaca aatcattacc gtaattacaa tggcagaata tcagccaaaa tactcctgca
     1201 tcagagattt cttcaaagtt tcaagttctt ccagagattt ttggattgcc aatttagatt
     1261 tggtgtcttg ggacgtaata ttaccatacc tgaatttata cgtaaatgtt tatattttat
     1321 taattcatcg tagtacgcta catttccaac agtacctctt gttgtcaaga ctatatcata
     1381 acgctcaagt ttgccttttc gaagtaattt atcctttgtt ttagtgataa attgctttgt
     1441 atcgaatgaa aatccgtttt tagtaacatt ctttgtattt aaaaataaac agtactcctc
     1501 actaaacaac tcatctgatt taggataatt tttgccccta tcaccatcta taatatcaaa
     1561 taagttatca atgctttcaa atattttatt ttccccaaac atctcgttaa atcgggattt
     1621 gcttttaaga gatgaatttt tggtgtgaat gcttgacatt attttgattg accatggcat
     1681 aggccagggt tgtgtcaatc ttgctatgac ctagcagttt ttggacttgt tcgataggca
     1741 tacctttatc aattgcctta gtcgctaaag ttcttctgaa cttatgtggg tgaacctttt
     1801 gtatgccgag tttgtctcct aactttctta agcgaatctc tacaccagca attccaagtc
     1861 tctgggcttt tccaactagc gttacaaaaa gagcagggtg actatctttt ctgtcgttaa
     1921 gataatttct taaatgaatt ttcgtccgag cgtcaaaata tactggtctc tccttctttc
     1981 ctttaccaaa gacaacacac tctctgtttt caaaatcaat atctgaacga ttcaactgta
     2041 caagctcccc tacacgcata cctgtcgatg ctagtaggtc tattattgcc aaatctctca
     2101 aattttcaca gttatcacgc ataatttcca aatgttcatc agtataagtt tccttcacat
     2161 tttgctcagt cttaattttc tttatccgtc gaatgggatt tttaatgata tattcctctt
     2221 gctcaagcca agc
//
LOCUS       DAVKLW010000111         2224 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKLW010000111 DAVKLW010000000
VERSION     DAVKLW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.111"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..424)
                     /locus_tag="VW324_002065"
     CDS             complement(<1..424)
                     /locus_tag="VW324_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727607.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYP"
     gene            complement(438..1151)
                     /locus_tag="VW324_002066"
     CDS             complement(438..1151)
                     /locus_tag="VW324_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9727608.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1611..1991)
                     /locus_tag="VW324_002067"
     CDS             complement(1611..1991)
                     /locus_tag="VW324_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9727609.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
BASE COUNT          736 a          429 c          344 g          715 t
ORIGIN      
        1 aaggataata ctctttaata ttattaactt tttgttcgta ggcatatttt gctacatgat
       61 ctaggtatat atcaaaaaag tcatgaggaa aatctcccat acagccatta cgattcatat
      121 tgaggctata ttttcgatta gtaagataat tcggaaatat atttcctatt actctaagat
      181 tatctacttg tgaatccctt ctcccaatat aatccccttc tttgactaat gatggagatt
      241 tccaagtaaa aattaaatcg ccagagaaat ttttcttatc aaaaaactta aaaagagatt
      301 gataaatact atttgctaat tgacaagtgt cagatgtaaa ttcataatta acagcttcat
      361 taaaatcaac ttcagacatt tcaatgtact cttcaatagt aatattagct ttttttgtag
      421 tcattagcta atctccttta aaatgtgatc ttttcaagaa tatttttatc taaacaatcc
      481 agcaagtctt ggtaagaata gacttcgtaa gtcggctggg cttgtgtgtg attttcgagg
      541 tgatgaggat tataccagat agtgtcaatc cccgcattat tgccaccttg aatgtcggcg
      601 gttagagaat ctccaatcat cagcgtcttt tctttactaa atccagcaat ttgctggcca
      661 atcttttcat aaaagagagc atccggcttt tgagtttgca actgttctga gataaagact
      721 tgattgaaat aaggtgctag accagattga gccaaacgtc ctgtctgaat ggcagtaatg
      781 ccatttgtcg cagcatacaa gtcataatca cgctcaatga ggctgtccaa gagatcatga
      841 gcgcccgata gtgtttgtcc ctgctgggcg aggtaaaatt ggtaacgctg ggcaagaaaa
      901 ctaccgtctt tttcctgtcc aaaatgagaa aataaacgag aaaagcgcgt gttaaccagc
      961 tcttgtttac tgattttctt cagctccaag tctttccaga gagccttgtt cataggaacg
     1021 taataatctt tataagcctg aatatccgca actccttctt cttttagaag ttgggtcaaa
     1081 gccacatcct cagcagcatc aaaatcaaga agagtgtggt cgaggtcgaa gagtagaaat
     1141 ttgtagaaca atttgaggtt ttcctttctg aaaattcatt aagaacatta tatcataaag
     1201 cacctcatac aattaactaa tttaatcact taaaaaaaat tcgaacactt tctatacaac
     1261 tgacagctca aatctttcag aatagaacaa tactaactat cgagcacccc cgtcttcata
     1321 aatatatgtg taattctaga cctagaattc ctataaacta aatgctttca tactcttcca
     1381 agtaattgat tgccttaaat tttaattttt gaaggattct aaagctagaa tagccccatc
     1441 acaatcagtt ttgattgatt cacaatttag aaacactata gtttcactcc tgttaaaata
     1501 aaaaggaact gcataaagca atccctttct aattttgaaa tcatttactt aacattttat
     1561 agttgagata atcaatagct tacctataaa aagagttata gtaaaattcc ttatttattg
     1621 atttcaagct ccgctaactg tgtttgaata actgacagtt ctgcaccagc ctgcagaaga
     1681 gcagctgcat tataggcacc ttctacaatt ggaaccctgt tgatgatgat acttttatca
     1741 ctgaaatcag tcaccatttt taagttcatt ttagcagaac ctaggtcaaa aaaggcaagt
     1801 aaagtatctg ctggattttc ggaaacaacc ctatctactt gatcaaaact cgttccaatt
     1861 cctccgccct cggttcctcc tacataagta atcggaacat ctttagctac tttactaatc
     1921 agttcaacaa caccttctgc aatgtgtttg gaatgtgaaa cgataacaag accaatacca
     1981 atactttcca tcaaaccacc ccagtttcta aaatagcagt aaagagtaat cctgatgaga
     2041 atgatccagg atcaatatgt ccaagaaacc atatgctcct aagacaagag ccaacagact
     2101 ggtcatcaat aatagtattg ttcttttttt catcattact ccttaactag tgtttaactg
     2161 attaattagc cagtaaatag tttatcttta tttacactat ctgttaagat atagtaaaat
     2221 aaga
//
LOCUS       DAVKLW010000112         2034 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKLW010000112 DAVKLW010000000
VERSION     DAVKLW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.112"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            4..1596
                     /locus_tag="VW324_002068"
     CDS             4..1596
                     /locus_tag="VW324_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727610.1"
                     /translation="MKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMNLERFLKLS
                     IYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLS
                     WLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKI
                     FASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQ
                     RKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGE
                     LGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEI
                     LKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMV
                     SNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDD
                     GRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVT
                     HAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          719 a          240 c          372 g          703 t
ORIGIN      
        1 aatataaaaa aatttagttt attaacgata tttattgttg ctggtcaatt attgctaatt
       61 tatgcagcaa ctattaatgc tctggtgttg aatgaattaa ttgcgatgaa tttagagcgg
      121 tttttgaaat tgtcaatcta ccaaatgatt gtctggtgtg ggataatatt ccttgactgg
      181 gtagtgaaaa attatcaggt tgaagtgatc caagagttta atctagagat tcgaaataga
      241 gttgccacag acatctctaa ctctacctat caagaatttc atagtaaatc atcaggaaca
      301 tatctttcgt ggctaaataa tgatgttcag actttaaatg atcaggcttt taaacaactt
      361 tttttagtaa taaaaggaat ttctggtact atatttgcag ttgtgactct taatcactat
      421 cattggtcat tgactgtagc caccttgttt tcattaatga ttatgctact tgtaccaaaa
      481 atctttgcat cgaaaatgcg agaagttagt ctaaatttaa ctaaccaaaa tgaagctttt
      541 ttaaaatcta gtgagactat attgaatgga tttgatgtgt tagcatcctt gaatctttta
      601 tatgtattgc ctaagaaaat taaagaagca ggaattttat taaagatggt tatacaaaga
      661 aagacaactg tagaaacgtt agcaggcgct attagcttct ttctcaatat tttttttcag
      721 atatctctcg tttttttaac aggctatctt gcaataaaag gaatagtgaa aattggtact
      781 attgaagcaa taggagcact aacaggtgtt atttttacag cgctaggtga attaggaggt
      841 caattatcct ctattattgg tacgaagcct atttttttaa aattgtattc aattaatcca
      901 attgagtcaa ataaaatgaa tgatatcgaa ccaaatgagg tgaatagaga ttttccgtta
      961 tatgaagcaa aaaatatttg ctataagtat ggagataaag aaatattaaa aaacttaaat
     1021 ttttgttttc aacgtaatga aaagtattta attttaggtg aaagtggaag cgggaaatct
     1081 acattattaa aattattgaa tggctttttg agagattata gtggagaatt gcgattctgc
     1141 ggggatgata taaaaaaaac ctcctattta aatatggttt cgaatgttct atatgtagat
     1201 caaaaagctt atttgtttga aggtacgatt agagataata ttttattgga agaaaattat
     1261 actgatgaag aaatactaca gtctttagag caagttggtt tgagtgtaaa agattttcct
     1321 aataacattt tagattatta tgttggtgat gatgggagat tactgtcagg agggcagaaa
     1381 caaaaaatta ctttagctag agggctaatt agaaataaga aaatagtatt aattgacgag
     1441 ggaacttctg ctatcgatag gagaacttcg ttagcgattg aacgtaagat attagataga
     1501 gaggatttga ctgtcattat tgttacccat gctccgcatc cggaacttaa acaatatttt
     1561 actaagatat atcaatttcc aaaggatttt atttaagaat ataggggagg gaaacatgag
     1621 attctattgt gaagcttatg aggtaattac tgaagagaaa aaagtatact ttaatgatat
     1681 tgagttagaa gttaagtatt cctctgtcga agaattgttt ataatatgtg agaaattatt
     1741 agataagaaa aaagtatcat ttttttatgt agatgagaaa cccttaaggt atctgttatt
     1801 tgactatata tttctactag ttctagctaa aaaaatatcc caatcttatg aactgcaccc
     1861 caaaagttag atttattctg tctaactttt ggggtgcagt tcattttgca tagttttggc
     1921 attcatccta gatatccata taaaatgtct gagattgttg aaatgtctaa aaaaatatca
     1981 gatctaaacg ataaaaaaga atttcacaaa aagaactgtg agttgaggtt tcaa
//
LOCUS       DAVKLW010000113         1805 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKLW010000113 DAVKLW010000000
VERSION     DAVKLW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.113"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            309..1655
                     /gene="gdhA"
                     /locus_tag="VW324_002069"
     CDS             309..1655
                     /gene="gdhA"
                     /locus_tag="VW324_002069"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9727611.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          559 a          341 c          355 g          550 t
ORIGIN      
        1 tggtataaat attgctttcc tccaaaatgg tcataatttt actagcaaat ctaacatatc
       61 acggataaat taacaagtga tttctgaatt gctaaacatt ttcttttctt atagcatacc
      121 atactttaag attttgtctt tgagaaagat atttccaaga aaaacgttcg tttttttatc
      181 tattttcaaa aaaaaccaaa aactgaattg aaagaatttt tagaaaattt ctgttttttc
      241 tttacagtga aaaaaaattc tgctataatg tcttatgtaa taaaatatga caataaaagg
      301 agaacgatat gacatctgct aaagaatata tccaaagcgt gtttgaaact gtaaaagctc
      361 gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga atttttcaac actttggaac
      421 ctgtatttga aaaacaccct gagtatatcg aagaaaatat cttggcacgt attactgagc
      481 ctgagcgcgt gatttctttc cgtgttcctt gggttgaccg tgatggaaaa gttcaagtaa
      541 accgcggcta ccgtgttcaa ttcaactcag ctgttggacc atataaaggc ggacttcgtt
      601 tccacccaac tgtaaaccaa gggattttga aattcctcgg atttgaacaa atctttaaaa
      661 acgtcttgac tggacttcct atcggtggag gtaaaggtgg atcagacttc gatcctaaag
      721 gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt catgactgaa ttgcaaaaac
      781 acattggacc atcacttgac gtacctgctg gtgatatcgg tgttggtgga cgtgagattg
      841 gttaccttta cggtcaatac aagcgtctta accaatttga tgctggtgtc ttgactggta
      901 aacctcttgg atttggtggt agcttgattc gtccagaagc aactggttac ggtttggttt
      961 actatactga agaaatgctc aaagctaacg gtaacagctt tgctggtaag aaagtggtta
     1021 tttcaggttc tggtaacgtt gctcaatacg ctcttcaaaa agcaaccgaa cttggtgcaa
     1081 cagttatctc tgtttctgac tcaaacggct atgttatcga tgaaaacggt atcgacttcg
     1141 atcttttggt tgatgttaaa gaaaaacgtc gcgctcgttt gactgagtat gcagctgaga
     1201 aagcaactgc tacttatcat gaaggttctg tatggactta cgctggaaac tatgacattg
     1261 cgcttccatg tgctactcaa aacgaaatca acggtgaagc agctaaacgt ttggttgctc
     1321 aaggcgttat ctgtgtatct gaaggtgcca acatgccaag caaccttgat gccatcaaag
     1381 tttacaaaga aaatggtatc ttctacggac ctgccaaagc tgccaacgct ggtggtgtag
     1441 ccgtttcagc ccttgaaatg agccaaaaca gccttcgcct ctcatggact cgtgaagaag
     1501 ttgatggacg tctcaaagac atcatgacca acatctttaa cacagctaaa acaacttcag
     1561 aaacatatgg tcttgataaa gactaccttg caggagctaa cattgctgcc tttgaaaatg
     1621 tagcaaacgc tatgattgca caaggcgtgg tataaaaaaa aattacggaa actgactacc
     1681 cagacgggta gccagttttt tcaattccta aaatttaaga ttctaatacc ctttatgcaa
     1741 aaatatagaa ttctaccttt ttctttatat aggctaatgt ttttaatata tttattgtct
     1801 atttt
//
LOCUS       DAVKLW010000114         1716 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKLW010000114 DAVKLW010000000
VERSION     DAVKLW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.114"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(9..1223)
                     /locus_tag="VW324_002070"
     CDS             complement(9..1223)
                     /locus_tag="VW324_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9727612.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1379..1673)
                     /locus_tag="VW324_002071"
                     /pseudo
     CDS             complement(1379..1673)
                     /locus_tag="VW324_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          531 a          386 c          334 g          465 t
ORIGIN      
        1 tgcaagccct atctacgata ctgtttcaag aaacgagtca tcttttcttg gatttgggct
       61 aactcatcaa cacgaggaag gtaaacgata cggaagtggt ctggttcctg ccagttaaag
      121 cctcgaccat gaaccaagag aaccttttcc tgcttcaaga aatcaaggac aaactgctca
      181 tcatcatcga tacggtacat attgcggtcg atttttggga agatatagag ccccgccttg
      241 ggtttaacgg cagacaaacc tggaatatct tgaatggcat tatagatgaa atttctttgc
      301 tcgtagattc gtccaccagg aagaagcaat tcatcgactg attggtgacc ccccaaggaa
      361 gtttgtacga cttgttgggc caaaacgtta gagcaaaggc gcatattgga cagcatattg
      421 agcccttcga tatagccctt aacatgagtc ttagggccag acaagaccat ccaacccaca
      481 cggaaacctg ctatgcggtg ggattttgac agaccattca tgctgacaca gaagacatct
      541 ggtgccaagc tcgccacagg cgtatgcaca tgtccgtcca ttaccatgcg gtcatagatt
      601 tcatccgcaa agataatcaa atcgttttga cgggcaatct caataatctc cagcaagagt
      661 tccttaggat aaagggctcc agttgggtta tttggattga taaggacaat tgccttggta
      721 ttggaagtga tttttgactt gatatcgtca atatctgggt accattccac agcttcatca
      781 cagatatagt gaacggcatt tcctccagct aggctgacag ccgccgtcca tagaggatag
      841 tctggcattg gcactaagac ctcatcccca ttgtccaaaa gcccctgcat ggacatgaca
      901 atcaactcac tgacaccatt tccaaggtaa atatcatcaa tatctacgtt tgggaatttc
      961 ttcagctggc aatactgcat gatggcctta cgggctgaga aaatcccttt ggagtcagaa
     1021 tagccttcac tatcacgcgc attcataatc aagtcatgaa tgacctcgtc tggcgctgta
     1081 aagccaaatt ctgctggatt tcctgtattc agacgtaaaa tcttttctcc attcgctcgc
     1141 atccgcatgg cttcttccaa tacaggacca cggatatcat aagcaacatg ctctaactta
     1201 ctagacttgt tatattcttt catcttgttt cctcaattca tcaggatagt aacattatac
     1261 cactagaaag agaatgcgac aagtttactg aaatgtgtta ctagtttcgt atagaagaaa
     1321 aaccttgcta aaggcgaggt taagaacgta gagatagcag gtattttgca aagacacatc
     1381 acataccaaa agataaacct agaaaaaagc aagcctagaa aggagtcata aagaagatta
     1441 aacatgagga aaagccccca tatcatcaag taatcccaac gcccacttcg ttttgcaact
     1501 agaaataaga ttagatggta gagtacatga aaaactaaaa atccaataaa acttggataa
     1561 agaagcggta ccagactctc taattgacag atcatgatca cttctaccaa gactgaaact
     1621 aggataattc cataataaag gaaatttgat ttttgaaaag agtttttcat cattctccct
     1681 tccttcactt ctttcctgct atcctttctt ttattt
//
LOCUS       DAVKLW010000115         1636 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKLW010000115 DAVKLW010000000
VERSION     DAVKLW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.115"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            84..491
                     /locus_tag="VW324_002072"
     CDS             84..491
                     /locus_tag="VW324_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9727613.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VW324_002073"
     CDS             656..955
                     /locus_tag="VW324_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9727614.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VW324_002074"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VW324_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9727615.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          556 a          325 c          285 g          470 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatca atttgtcctg
     1621 ttcttgtttc atttta
//
LOCUS       DAVKLW010000116         1605 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKLW010000116 DAVKLW010000000
VERSION     DAVKLW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.116"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..280
                     /locus_tag="VW324_002075"
     CDS             <1..280
                     /locus_tag="VW324_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEU9727616.1"
                     /translation="EKKEESIKQLQNQKRQLKKKLNEQERKARNKRLIEKGAVFESIF
                     EESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKDEIT"
     gene            595..>1605
                     /locus_tag="VW324_002076"
     CDS             595..>1605
                     /locus_tag="VW324_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEU9727617.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIYDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKT
                     NKE"
BASE COUNT          764 a          194 c          273 g          374 t
ORIGIN      
        1 tgaaaagaaa gaagaaagca ttaagcaatt acaaaatcaa aaaagacagt tgaagaaaaa
       61 acttaatgaa caagaaagaa aagcaagaaa caaaagactt atagaaaaag gtgcagtgtt
      121 tgaaagtatt tttgaagaaa gtattgattt aacaaaagat gaattttata agttaataaa
      181 aacgttaaat gatgaagaaa taagattaaa cataatggaa attttagaag aaagaataga
      241 tgataatgta gaaaaatcat ctaaagatga aataacataa ttgtcccttg gttacaaggg
      301 cgcaattata caccctaaag ggtgcttgcg tcctgcggag tcaaaccctt gcagggagca
      361 ataaccattc gctttttaaa agtcaagggt aaataaactc gctaaagctc gcccttgact
      421 ttttaaaatt tgaaatgaaa gtaacaatta agccgacata ctgaaacaac aaaaattaat
      481 tcagtagtcg gcttttttaa ttttatcatt tcaaaacgct cattcacaaa gaggatataa
      541 aaaaactatt aagcctccac ctaaaacaac aaagaaagaa aggaagtgat aaaaatggca
      601 gacagttttc atttttcagt aaacataata tcaagaggaa aaggcaaaag tgcagtagca
      661 agtgcagcat atataagtgg agaaaaaata aaaaatgaat gggacggagt aacccatgac
      721 tacacaagaa aagaaaaaat attagtaaaa aatataatat tgcctgatca tataccaaaa
      781 gaatttaatg ataggtctac cttatggaat aaagtagaaa tggcagaaaa aaattctaat
      841 gcacaactag caagacaatt cataatagga ctaccaaaag agttatcttt aagtgaaaat
      901 aaaaacctgg ttgaaagata tataaaagaa aatttaacat cacaaggaat gatagtagat
      961 tatgcaattt atgatgagag tcaagataaa aatggaaata tccattgtca tataatgacc
     1021 ataatgcgac caataaacga aaaaggagag ttcttagcca agtcaaaaaa agaatatatc
     1081 ctagatgaaa aaggagaaaa agtattaaac aaaaatggaa aaccaaagac aagaaaagta
     1141 gaactaacaa cctggaacga tacaggcaat gtagaaaaat ggagagaaaa tttttcagat
     1201 ctttgtaaca aatatttaga aagagcagga gcagaaaaaa gagtagacca taggagtttt
     1261 aaaagacaaa attcagatta tctaccgaca atccatttag gatcagcagc aagtgcaatg
     1321 gaaagaaagg gaatagaaac tgacaaggga aactataata gagaaataag aaaatataat
     1381 caacttgtaa aaacaataaa agaagagata aaaacattaa agggttggat aggaaattta
     1441 ttagataact tatctactgc ttatgaaaaa tttaaggata tcgaaagaga taaggtaata
     1501 gacaacccta aactttttaa tctaacaaat tatctattaa cttattcaga aattcaaaaa
     1561 gaaaaaagta aatatctaaa aggatatgca aaaactaata aagaa
//
LOCUS       DAVKLW010000117         1475 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKLW010000117 DAVKLW010000000
VERSION     DAVKLW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.117"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            4..>1475
                     /locus_tag="VW324_002077"
     CDS             4..>1475
                     /locus_tag="VW324_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEU9727618.1"
                     /translation="MEENYNLSITQIKNSIKENSLVLFVGAGISANSNLPTWGELIQS
                     LKKELNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPNELHKLIEKIA
                     PKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGDFSKKNIVLKE
                     DDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNTFKLDAKNAYF
                     YTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLEVLSENRSQDV
                     NNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQLRFEIDGHEE
                     LRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKAKFRELANIAF
                     RQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQILNSVTGDEKKII
                     EFFRDSILNFNFLYRKLETINELFDEIREEHESYRRGGWSA"
BASE COUNT          560 a          170 c          266 g          479 t
ORIGIN      
        1 aatatggaag aaaattataa tttatctata actcaaataa agaattctat taaagaaaat
       61 tccttagttt tgtttgttgg tgcaggaata tcagctaatt ctaatctacc aacttgggga
      121 gaattgattc aatcattaaa aaaagaattg aacataccag aagaaagaac agatagtcca
      181 ctgagaattg ctcagtatta ttatgatact tttgggaaaa atcagtacac aaagaagata
      241 gaagaaattt tctttaagaa gggattgagt aaaccaaatg agttacataa attaatagaa
      301 aaaattgcac caaaacatat tattacgaca aactatgatt cacttctgga gagtcaattt
      361 gaaagtggct tgttaaagta caatgtagtc gctgaggata aagacatccc ttatacgagt
      421 tcggaaagat acttgattaa aatgcatggt gacttcagta agaaaaatat tgttttgaaa
      481 gaagatgatt atttagacta tcatttgaac ttcccaatga tttctacttt aatccagtca
      541 ttgataatga atcatactct attgtttgtg gggtattctc taagtgattc gacttttaat
      601 tcaattttta gaatgattca aaatacattt aaattggatg ctaaaaatgc ttatttttat
      661 actcccgagg aaccgtctat gatcattcga gagtattata agaagcaagg aattttcatt
      721 atctctaatg aagagaatct aggtcaggag acatcagaaa aacagaacaa attgtattgc
      781 aggacaaaag attttttaga ggttctatca gaaaaccgaa gtcaagatgt aaataatgct
      841 gatgatttat ggaatcaact ggctttttta gatagactta gttttattga tgcgaaagat
      901 ttttctagat attctgattt aaagaaaagg gctttaaact gggatgatga gtattgctgg
      961 tttggaaatg atcaattaag atttgaaatt gatggtcatg aagaattacg aattatggta
     1021 tctaaaaaat ctttattaaa tcgctttcta gatatggaga ttggtgagcc tagggattta
     1081 aaggggaatc gattcttaag taaggctttt aagttgtatg aagaaaaaca atattcttta
     1141 gcaaaagcaa aatttagaga actagctaat atagcatttc gtcagaaaga ctattttaat
     1201 ttcttagttt gtgaatttaa ttttcagcaa attcaaataa ttgatagata cgaaaaaaca
     1261 cctagttatg cagagccact ttatgatgga gaattaagtg aattaacaga acaaattctt
     1321 aactcggtta caggagatga gaaaaagatt atagaatttt ttagagattc aatccttaac
     1381 ttcaattttt tatatagaaa attagaaact attaatgaat tatttgatga aattcgtgaa
     1441 gagcatgaaa gctatagaag aggaggatgg tctgc
//
LOCUS       DAVKLW010000118         1473 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKLW010000118 DAVKLW010000000
VERSION     DAVKLW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.118"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..695
                     /locus_tag="VW324_002078"
                     /pseudo
     CDS             1..695
                     /locus_tag="VW324_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            741..1187
                     /locus_tag="VW324_002079"
     CDS             741..1187
                     /locus_tag="VW324_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9727619.1"
                     /translation="MNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMPMKFNTII
                     SEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQII
                     IAHRLSTIKDADIIFVMKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            1200..1370
                     /locus_tag="VW324_002080"
     CDS             1200..1370
                     /locus_tag="VW324_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727620.1"
                     /translation="MKKVNEYVISTAASLGVMIGIVFAIFLDFPVEYAISLGLLNGIV
                     LGSLIFYKNNKN"
BASE COUNT          560 a          163 c          273 g          477 t
ORIGIN      
        1 tgtttagtac tagaaaaata atgtatgata cggttaatag agaattacaa gaacaatcat
       61 tgatttactc agtagaaaca gaagcattaa atactatttc ttcaataaaa atatcgggat
      121 tagaagatga aatatatgaa aactggtcta agtatttaaa aaacgtattg acaaagtata
      181 aaaaacgaag tattgtgcat attttataca attcagctac taatgtattt caattgttcg
      241 ctcctataat tatattgatt tttggacttg ataatgtttt aaatggaaaa attttattgg
      301 gtgaggttgt cgcatttcaa accatggcat caattttatt tagttcagaa atctcaatat
      361 ttaatgctta tacgcagtat attttggcag caggatactt aaatagagta aatgatatct
      421 ggcttgaaaa tgaagagaat gttgaaaatg gtcttaaaaa gtgttcttta gaaggtagaa
      481 tagatattaa ggacctatct ttctcttatt ccaaagactc agctcctgta atagaaaatt
      541 taaatttgac tattgagcca ggacagagaa ttgcactagt cggtcaatcg gggtcaggaa
      601 aaagtacgtt atcaaaaata ctatcaggat tatataagat tgatacagga aaaattttat
      661 ttgatggtgt aaatattaat caaatagata aaaaaatatt aagtcaaaat ttaggagtag
      721 ttccacagga ttcattttta ttgaaccgaa gtattcttga taatataact ttaaagaacg
      781 aagttacttc acaaaagata gaggaagttt gtaaagcagt tcaaatctat gatgaaatca
      841 tggctatgcc gatgaaattt aatactatca tctcagagat ggggtcaaat atttcaggtg
      901 gacaaaggca acggatagca ctggcacgtg cattaataaa taatcctagt attgtaattt
      961 tagatgaagc aactagtgca ttagacacta ttaatgagga aagaataaca aagtatatac
     1021 aaagtcaggg ttgtactcaa ataattatag ctcatagatt gtcaacgatt aaggatgcgg
     1081 atattatttt tgtaatgaaa ggtggtaaga ttgttgagtc aggaaatcat aagtacttaa
     1141 tggatcttgg tggagagtac tacagcttat atacaaaaag gaaatgaggt gtaaagaaaa
     1201 tgaagaaagt aaatgaatat gtaatttcaa cagcagcctc actaggggtg atgattggaa
     1261 tagtatttgc aattttttta gattttccag ttgaatatgc tatttcttta ggtttgttga
     1321 atggaatagt attgggttcg ctgatttttt acaaaaacaa taagaattaa gcataatttt
     1381 ttgctgtaaa ctaaggagta gagatggcta tagttgaaat tataaatcta acaaaaagct
     1441 ttaaagatat tgaagttatt cataacactt cat
//
LOCUS       DAVKLW010000119         1427 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKLW010000119 DAVKLW010000000
VERSION     DAVKLW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.119"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            20..277
                     /locus_tag="VW324_002081"
                     /pseudo
     CDS             20..277
                     /locus_tag="VW324_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831365.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            351..524
                     /locus_tag="VW324_002082"
     CDS             351..524
                     /locus_tag="VW324_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727621.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            710..1237
                     /locus_tag="VW324_002083"
     CDS             710..1237
                     /locus_tag="VW324_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEU9727622.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1364..>1427
                     /locus_tag="VW324_002084"
     CDS             1364..>1427
                     /locus_tag="VW324_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU9727623.1"
                     /translation="MATNKRYYWIKLKEEFFTDKR"
BASE COUNT          755 a          183 c          219 g          270 t
ORIGIN      
        1 tgaagaaaaa attcttttaa tgtaaaatct tgaagaaata aataaagaaa aagacaagat
       61 aaaagatgaa taccaggaaa ttaatcatat aaaatattca gtagattttg taaacaaaga
      121 acttggtata gatttatcca tagaaataga taagctaata aaacagggag aaaaaccaag
      181 tgtaatagca cagattaaaa agttccaaga ccaagttaat aaagataatg aatacagaga
      241 aatgatgaaa aacaagaaaa tggatcaaga aagataagac ctggtaaaaa tatcttatcc
      301 aagctataat atgaccaaac aactaaagtc agatctagga ggtaatagag atgaataaaa
      361 ggcaagagct aatagatgaa ctcataaaag caaatgaaga tggaacatac aaaacctata
      421 aaagcacaga agaaataaaa gcaatgaaca atgaagaaat acagattata tattccaaca
      481 tgaaaaacta tctatcagac aaaagaacac acataaacta ttaaaggtgg aaaggtagtg
      541 taagctattt ttaaaagtat aaaaggtaca aagacctaag aaatatataa aaacatcaaa
      601 ataaagtatt ccaccaccaa gaacaataaa ataaaaacaa gcaaagggaa gtatagaaaa
      661 attaaaacct atacttccct tttttaattc aaacaaagga gagaaaagca tgataaacga
      721 agaaataagc aaggaagcag gtcaagcagc acaaaccata atatcataca caataaaggc
      781 aacaaaagaa tcaatcaatt tagaaaaaga aataagaaaa aagatgaatg aaactttaga
      841 aaaagcaaat ggaaacttaa aaagtcttat gggcgatgaa atgaaaataa aagatctcta
      901 caagaaagga caactagaaa atataagcat agatcaaatc gacctcaaag acttaaaaaa
      961 agaactaaac aaacttggag taagtttctc agtaatgaaa aacaaagaaa gcaaaaacta
     1021 tgaaatattc ttccaagcca aagacataaa agtaatggaa tatgccttta agcaagtcat
     1081 agccaaggaa aataaaaaag aaaaagaaag tatcctaaaa caaataaaga aatacaaaga
     1141 cctatccaaa aacaaagata agacaaaaga aaaaggaaaa aggaaagtaa aagaaaaagt
     1201 aaaaccaaac aaaaaagata tgaccagaga aatctaaagg gagtaacgaa aagttacacc
     1261 cttaaataac cataataaca agacttccga aaaacagaag tccagaattc cgaaaatcgg
     1321 aaatcaagaa tttcgattat cgaaaatcaa gaaaggagca gatatggcaa caaacaaaag
     1381 atactattgg ataaaattaa aagaagaatt tttcacagac aaaagga
//
LOCUS       DAVKLW010000120         1382 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKLW010000120 DAVKLW010000000
VERSION     DAVKLW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.120"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            83..1261
                     /locus_tag="VW324_002085"
     CDS             83..1261
                     /locus_tag="VW324_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9727624.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          512 a          251 c          200 g          419 t
ORIGIN      
        1 cttaaaaaaa ttaacatcta catttatcca tagcaaaaaa tcataggatt ttaagaatct
       61 gacaactagg aagaaaacta atatgacctt atctaagaaa caattacaac taagagctaa
      121 aatactggaa acagtttaca cattaggtcc catttcacga attgaaatcg ctactaaaac
      181 aggaataaca cctgcaacaa ctagcagtat cacaaatgat ctgattaaag aaaatattct
      241 cttagaactt ggtgaagacg aacacgatac aagcgtaggg agaaaaaaaa tcctgctaga
      301 cattcaagct aaacgatttt actacattgg ttgtgaacta tctgaaaaac atttcacttt
      361 tgctttagga gataatctag gaaatatctt aaaggaagag aaagaaattg ttactaaaca
      421 gttaatccaa gaaaagggaa atcaactcat taaccagaca ctaaagcaat tcttaaataa
      481 ctgctctgat tatgaaatag aagcaattgg aatcgcgcta cctggtcgct atttagataa
      541 ttataaaata acaacaaaca atcctttatg gcaacatatc gacttagaaa tgattcaatc
      601 ccactttgat aagcctttgt ttttctctaa caatgttaac tgtatggcta taggtaaacg
      661 cctatttagt cgccaacaga atgaccctaa ttttgcttat tttcattttg ctagaggtat
      721 gcactgttct tatatctacg acggaaacat ttatgggaaa ggaaatctaa tgattggaga
      781 aatagggcat acagttgttt catctgaggg agaagaatgt agctgcgggc gcaagggatg
      841 cttacaaact tttgctggag aatcttggtt aatcaaaaaa tcaaaaattc tatatcatca
      901 atctccctat tctctacttc ctagtttagt caaaaatgcc gatgatattg atatccaagt
      961 tattttaacc gcttatcaat taggtgacac tggaataatc acacttatcc atcaagcctt
     1021 actctatctt agtcaaacca ttttaaatat cagtatgatg attgactctc aaaaaatcta
     1081 cctacatagt cctttactaa caaatcaaca catcatccaa aaactatata gtgaaatgaa
     1141 ctacaaaccc aaactgctct ataatcgcct acctgaagtc attattgaac cttacaatga
     1201 ttttacagcc gcacactcag ctattgcact ctgtttgtat catacaattc tacattctta
     1261 attttctatt acatttaata aataggctaa aaagagggag tatatcataa aaagcgaata
     1321 aacctgtttt ctgaaaatca gaattctgtt ttgttcgctt tctttatatt tggaattata
     1381 ct
//
LOCUS       DAVKLW010000121         1340 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKLW010000121 DAVKLW010000000
VERSION     DAVKLW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.121"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            347..946
                     /locus_tag="VW324_002086"
     CDS             347..946
                     /locus_tag="VW324_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727625.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            1089..1259
                     /locus_tag="VW324_002087"
                     /pseudo
     CDS             1089..1259
                     /locus_tag="VW324_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          475 a          197 c          267 g          401 t
ORIGIN      
        1 agttgtttgg tcgcttttca ttatagatct tatgggactt tttttctaca ataaaatagg
       61 ctccataata tctatagtgg atttacccac tacaaatatt atagagccgt taatatttgg
      121 agaagaggat ggccatcttc tccattttta tgtgcagagt tatagtgact tgatgctggg
      181 aaagtacact gtgactgcta atactcaatg aaaatcaaag tgcaaactag gaagctagcc
      241 gcaggctgta cttgagtacg tcaaggtgaa gctgacgtgg tttgaatttg attttcgaag
      301 agtataaaac attcctagaa gttactttga tttccctaat ctatttatgc aaattttatg
      361 ttattttact ataacagttg ttgctaagcc aaataatagt ggggaagttc atttagacgt
      421 aagcattgaa gataatcagg gaggtagtgg gcataatttc agttctgttt caagtagctc
      481 acaaacagct aaatatgaag gaactggtta taataacaat tcatcattat atataacgat
      541 tgataaaacg tctgatgcaa cagctctttt gaaattaaag ttgaataatg ttgataatca
      601 acctgctact gaagttccta gttcaggaat tactgtaaaa ttaaatgcta aagataatgc
      661 tggaaactgg acaagtgctt cgaataaaaa agaagtaaca gtaaaaattg tttctgctaa
      721 accgacatat ccagacaaaa tcttagtgaa aaatcctgat aatataaaag atacagaaaa
      781 aaatgccatt attgaaaaat tgaaagaggc aaataaaaat catccagcag gagctccaac
      841 ctttgctaaa ggtgaaggag agcatgcaaa tgatattgta gcaacttatt cagatggtac
      901 aacttattat gtaccgttaa atgatgtgac aaaatatgcg aggtagtggc tgtaccactc
      961 acttattcac ctccccgtga tttgtagtag tgataggctt tctcactatt attataaaac
     1021 aaaataaaga tcacaacact ttttcattct gtgttgtgcc ttgagtgaaa cgaaaggaat
     1081 gaattataaa tatgaaaagt atagtcacta gcatagatga gcgcttgcgt actcgcctac
     1141 gagtgattat ctggaagcaa tggaagaaga aatcgagacg attatgggga ttgcttaagt
     1201 taggggttcc taaatggata gcagataagg tatctggctg gggcgaccac tatcaattat
     1261 attaaaatga aataagaata ggacgaatcg atcagggaag tcaaatcgat ttctaacaat
     1321 attttagaag tagaggtata
//
LOCUS       DAVKLW010000122         1240 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKLW010000122 DAVKLW010000000
VERSION     DAVKLW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.122"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..703
                     /locus_tag="VW324_002088"
     CDS             <1..703
                     /locus_tag="VW324_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9727626.1"
                     /translation="SVIIGSNGCGKSTLIKTLSRLIKPLEGEVLLDNKSINSYKEKDL
                     AKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVMANVEDLA
                     NNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKT
                     TICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPIS
                     NSPLMIPIGKHHVSS"
BASE COUNT          499 a          195 c          202 g          344 t
ORIGIN      
        1 atcagttatt attgggtcaa atggttgtgg gaaatcaaca ctcattaaaa ccttgtctcg
       61 acttataaag ccattagagg gagaagtatt gcttgataat aaatcaatta attcttataa
      121 agaaaaagat ttagcaaaac acatagctat attacctcaa tctccaataa tccctgaatc
      181 aataacagta gctgatcttg taagccgtgg tcgtttcccc tacagaaagc cttttaagag
      241 tcttggaaaa gatgaccttg aaataataaa cagatcaatg gttatggcca atgttgaaga
      301 tctagcaaat aacctagttg aagaactttc tgggggtcaa aggcaaagag tatggatagc
      361 tctagcccta gcccaagata caagtatcct acttttagat gagccaacta cttacttgga
      421 tatctcatat caaatagaac tattagacct cttgactgat ctaaaccaaa aatataagac
      481 aaccatttgc atgattttgc acgatataaa tctaacagca agatacgctg attacctatt
      541 tgcaattaaa gaaggtaaac ttgttgcaga gggaaagcct gaagatatac taaatgataa
      601 actagttaaa gatatcttta atcttgaagc aaaaattata cgtgacccta tttccaattc
      661 gcctctaatg attcctattg gcaagcacca tgttagctct taataattga atattggaat
      721 tcatttaaaa gtcgcaaatt ttgaactgac cctcaaaagt taggccaaaa aactaactaa
      781 aggaggtcag ttttttaatg gcaaatacag cacagaattt aagataaaat tagtaaaaga
      841 atatcatgaa tgaaaaatag gatatagaga ataacagcaa tattaaaaag atcaggactg
      901 ataataaaca aaaagaaagt acaacgacta gttcaaaagc taaaacttca agtaaaaagt
      961 tattcaagaa aatctagaaa atactcatcc tacaaaggac aggtaggaaa aatatcggac
     1021 aacaaaataa aaagaaatat ttattaccga aaaaaagggg aaatcgagtc aagtaccaac
     1081 aggctcatct attccaaaaa gatatttact cgatggaaaa actcctagaa ttagtgctac
     1141 aaatattgac aatggtatct taggttacta cgaagatata gatgataaaa attatagagt
     1201 ttttgaaaat tttatatctg tttcattttt aggagcagtc
//
LOCUS       DAVKLW010000123         1171 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKLW010000123 DAVKLW010000000
VERSION     DAVKLW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.123"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            230..>1171
                     /locus_tag="VW324_002089"
     CDS             230..>1171
                     /locus_tag="VW324_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU9727627.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFL"
BASE COUNT          410 a          206 c          243 g          312 t
ORIGIN      
        1 accttagtca agaattagat gtttcactat gtttgagtaa gttgatgatt tcatttgata
       61 acaggtttga aactgttagg ctaggtggcc aaggctaatc atagccttgg tttagctgaa
      121 aaaacaggtt caagggttcc tgttgtcaaa tgaaatgtga tttaaggtat aagaaaacac
      181 ctctgtgcta tacttgttgt tcaccacaaa cacaaggaaa ggcacagaga tgcaagaaca
      241 ttatacccca aaagggaaac atttgacaat agataaccgt cgcttgattg agcggtggaa
      301 gaatgaaaat aagtccaatc gtgaaattgc aggcttgtta ggaaaggcgc ctcaaacgat
      361 tcatactgaa gtcaaaagag gtacaacttt acaacaagtg agaaaagggc tatacaaaaa
      421 ggtctattct gccgattacg cacaaactgt ttaccaattc aatcgaaaac ggtcggtgaa
      481 aaagttaatt ttaacaaagg aaatcagaga gaagatctta cactatcata agcaaaaatt
      541 ttcgcctgaa atgatggtta acaagaagca agtgaaagtt ggtatttcaa ccatctacta
      601 ctggtttcat aatggtcatt taggattgac gaaggccgac atgctttatc ccagaaaaag
      661 gaaaggtgtc aagaagcaag ctagtccgaa ctttaagccg gcaggtaaat ctatcgaaga
      721 acgtcctgac gttattaatc ttcgcttgga aaatggtcat tatgaaattg ataccgtcct
      781 actgactaag ataaaaaatt attgcctgtt agtcttaacc gaccggcgga gcagacacca
      841 aattataagg ttaattccaa ataaaactgc tgaatctgtc aatcaggcgc ttacgttact
      901 attaggggag catcgtattc tgtccattac tgcagataat ggttcggagt tcaaacgatt
      961 gtctgaggta tttcctgagg aacatatcta ctacgcacat gcttactctt catgggagag
     1021 aggttcaaat gaaaatcata atcgattaat tcggagatgg ttacctaaag gaaccaagaa
     1081 aacgactccg aaagaagtag cttttatcga aaattggatt aacaactacc ctaaaaaatg
     1141 cttggactac aagtcgccaa gtgaatttct t
//
LOCUS       DAVKLW010000124         1120 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKLW010000124 DAVKLW010000000
VERSION     DAVKLW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.124"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..156)
                     /locus_tag="VW324_002090"
     CDS             complement(<1..156)
                     /locus_tag="VW324_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9727628.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMK"
     gene            complement(169..918)
                     /locus_tag="VW324_002091"
     CDS             complement(169..918)
                     /locus_tag="VW324_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9727629.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            complement(930..>1120)
                     /locus_tag="VW324_002092"
     CDS             complement(930..>1120)
                     /locus_tag="VW324_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727630.1"
                     /translation="FFNKIVNGLIQNIEENLEDDIERILKYLYICLFNEIFIIKNKVN
                     FFDDVEFNQTLSEFLDKL"
BASE COUNT          389 a          217 c          118 g          396 t
ORIGIN      
        1 cttcataagc tcttcatttt tagatggaag aatgcccttt tttaaattat ttatttttac
       61 agaatctata tcatatgcaa caactttata tttagatgca aatagtaacg cgtaggccag
      121 cccaacatgc cccaaaccaa ttactgctat attcataaaa ctacttcctt atttcttaat
      181 ccaaaatcta atagaataag ctgccccatt ccttaaatac aactctttaa tattgtttaa
      241 aagtttttca actgatttcc agattatcaa aatctgagat ttatagcaca atattgatga
      301 tattctatca atataatgtt tttcatcaag ttcctcttga tacattttta attctttagt
      361 ttttcccata taactaacca tactactatc acttacatat gggaagtcct cataatatat
      421 tactttataa cgcataaatt caagcgccct tccaatacta ttcacaaaaa catgatcaac
      481 atggtcacca agtgaaagcg gacaatatac gacacatttg tcgtctaaat gcattaacag
      541 ctcttttatg atatcattct ttaatgtgtc ctcatttttt aattcactat agatatgacg
      601 gtatagaaaa ttgccatttc tatctttcct atagagacat tcatagtacg ataagtgtct
      661 aaaatcacac tgtagacgtt cacaagctaa cctgtcttct ttcttccttt cttcaatcgg
      721 atatttccca aggttacaca acttatgaaa ttgcttagca gagggctgta gctgttggct
      781 caaagggtaa ccagaaaata tagtaataac aagtacaatt tctccttctg aagttaattt
      841 tgaaatataa tcaccacagg aaaaaattgc gtcatctaaa tgtggagata aaaagatata
      901 cttagtattg ttactcataa ccattccctc tacaatttat ctaaaaactc actaagtgtc
      961 tgattaaatt ccacatcatc aaaaaaattc accttattct taataatgaa tatttcgtta
     1021 aataaacata tatataaata tttcaatatc ctttcaatat catcctctaa attctcctca
     1081 atattttgta tcagcccatt tacaatctta ttaaaaaaga
//
LOCUS       DAVKLW010000125         1040 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKLW010000125 DAVKLW010000000
VERSION     DAVKLW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.125"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(25..87)
                     /locus_tag="VW324_002093"
     CDS             complement(25..87)
                     /locus_tag="VW324_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HEU9727631.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            167..1021
                     /locus_tag="VW324_002094"
     CDS             167..1021
                     /locus_tag="VW324_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9727632.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKVEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGEDKLKLHYTDH
                     FNKLVNKK"
BASE COUNT          385 a          165 c          136 g          354 t
ORIGIN      
        1 aaatacaata attaatacac ttttttaaat gatgataata tccatcacca aaaccaaaat
       61 cggtaaaaat tttgaaattt tcttcatact tggacttcct ttccgttttt atttattgta
      121 caataaataa aaacggattt aatttttgtt gggaaaatgg gaaattatga aatcaaaact
      181 tggcatcaca cttagaaaag ttcgtaaagg aaaacaaatt agtctatgct ctgtagcaga
      241 tgaacatctt tcaaaatcac aaatatctcg ttttgaacgt ggggagtcag agatatcttg
      301 tattcgacta atcaatattt tagacaaatt acatattact ttggatgagt tccttgtttt
      361 acacaatgac gactatacca gttccgaatc atttgctaat ctagtacagt acattcgtaa
      421 gcaatattca tcacaaagta tcaataatat agcatgtcta ctttctgata cttctgatta
      481 cactctaaat tcttttgaga aaacaatggt aaaatctatt ttacacacaa tggattcgaa
      541 catcattcca tcagatgaag agcttcttca tctgacagac tatcttttca aagttgaaaa
      601 atggggctat tatgaaatca ttttattagg taactgtgta cgcacaatta actacaactc
      661 ctacttctta ttaactaaag agatgttaaa taactatatt tactcatctc tgaataaaac
      721 taataagcag attgtctctc agcttgctat taattgcttc attctaagca ttgataagga
      781 agaattttct aattgctcct atttaattag taagataaag acattgttgg acaatgaact
      841 aaacttttat gaacaaacag tttttctcta tgcaactggt tactttgaat ttaaacgttg
      901 ccaatcaacc agtggaattg aaaaaatgaa acaagccata caagtcttcg atattttagg
      961 agaagataag ttaaaattac actatactga ccattttaat aaactagtaa ataaaaaata
     1021 aatgaaatta ttctctatct
//
LOCUS       DAVKLW010000126         1011 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKLW010000126 DAVKLW010000000
VERSION     DAVKLW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.126"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..559
                     /locus_tag="VW324_002095"
     CDS             <1..559
                     /locus_tag="VW324_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9727633.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
     gene            complement(513..>1011)
                     /locus_tag="VW324_002096"
     CDS             complement(513..>1011)
                     /locus_tag="VW324_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9727634.1"
                     /translation="NTGLSYKKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQF
                     ISSEQVYLKHNQLITPVATSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKF
                     LLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKVEKLFE
                     KVNQL"
BASE COUNT          361 a          163 c          151 g          336 t
ORIGIN      
        1 gaacaaagac gaaacaacct cttatcctat atatgaaatc ccagaagcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cttgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatacg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagttttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctatc aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat tttcaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgctcagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgctac aggtgttatt
      841 agctgattat gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa
      901 tcattatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt c
//
LOCUS       DAVKLW010000127          952 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKLW010000127 DAVKLW010000000
VERSION     DAVKLW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.127"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            105..584
                     /locus_tag="VW324_002097"
     CDS             105..584
                     /locus_tag="VW324_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9727635.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VW324_002098"
     tRNA            678..749
                     /locus_tag="VW324_002098"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
BASE COUNT          353 a          132 c          223 g          244 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aaacaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ttagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa agactgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc gtcaggagca cttgatgaaa
      781 aaaagtttaa aaaagcttaa aaatcttcaa aaaagtgttg acaagtgaaa gtggctgtga
      841 tatactaata tagttgtcgc ttgagagaag caagtgacaa agacctttga aaactgaaca
      901 agacgaacca atgtgcaggg cgctacaacg taagttgtag tactgaacaa tg
//
LOCUS       DAVKLW010000128          903 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKLW010000128 DAVKLW010000000
VERSION     DAVKLW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.128"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            1..788
                     /locus_tag="VW324_002099"
                     /pseudo
     CDS             1..788
                     /locus_tag="VW324_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            781..>903
                     /locus_tag="VW324_002100"
     CDS             781..>903
                     /locus_tag="VW324_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9727636.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVI"
BASE COUNT          331 a           81 c          188 g          303 t
ORIGIN      
        1 ccatggatgg gttgaatata acttaaaaaa gaaattcttg acttattttg atttttggac
       61 atattctatg ggagatgctt ttatttgtgt atctgaaact atgaaaaaaa gattggagag
      121 tataataaaa aaataagaag atagttgcaa taaataacgg aattaatgta tcaaatagcg
      181 atttagatgt agtaggtgtt caagacttta aaaaagaatt ttgtattcca aataacaaaa
      241 aaatcatttg ttatgttgga aggttggatc cagaaaaaag gccggataga ttccttgaat
      301 ttgcagaaaa attattttta gtcagagaag atgtgatttt tattatggct ggaaatggta
      361 gtatgtgggc tgctttgaaa gaaaaaattt gccatttgaa atgtagagat aatttcaggt
      421 tacttggtga aatctatcca gctactatag tttaccaaat atcggattta ttgtatattc
      481 cttctgatac agaaggaata cctatgtgtg ttttagagtc aatgtcacaa ggaactccgg
      541 ttttagctag taatgttggc gggttaagtg aaattattga acatagggtt gatggatttt
      601 tatttgagaa ggaagatgtt gagggagtgt gtgcttgtgc taatttttta ctcaatgatt
      661 ctgagtattt gaaatatata ggtgagaata gtaaatcaaa aataagaaaa catttttctg
      721 tgcaaaaaat gtttgtagaa accatgagag tatatgatga attattagag aagagtagtc
      781 atggatagga atatagatca agaattagta agtattataa taccaacaca taataggtat
      841 gaatctctga ttagagctgt gaaaagttgt ttacatcaaa gttataaaaa tatcgaagtt
      901 ata
//
LOCUS       DAVKLW010000129          788 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKLW010000129 DAVKLW010000000
VERSION     DAVKLW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.129"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(43..189)
                     /locus_tag="VW324_002101"
     CDS             complement(43..189)
                     /locus_tag="VW324_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727637.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            347..565
                     /locus_tag="VW324_002102"
     CDS             347..565
                     /locus_tag="VW324_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9727638.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          251 a          116 c          146 g          275 t
ORIGIN      
        1 cgaaatctag tggaatgaat ctggaatagt ccatcgagct ttctaatact ctttgaaaat
       61 ctcttcaaac cacgtcaacg tcgccttgcc gtgcgtatgg ttactgactt cgtcagttct
      121 atccacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgctcttt
      181 ggttttcatt gagtataaca cattgttaga agttggttta aatttcctaa tcagtttgtt
      241 cacatttacc ttcgatatat tatatcccat agttaaggtt ggtcatacag atgattatag
      301 ccatggagcc gtaaaactta gtgtttcttt agttgacaaa gatgccatga aaaaaatatc
      361 tgtaactgta ataggatatt ttgaaataaa tatagatgaa aatatcaccg atattctata
      421 cgtaaatggt actgctattc tttatcctta tttacgttct attgtttcaa tagtttcggc
      481 aattgatagc agtgaagcaa tgttgctacc taccattaat gttttagagt tactagataa
      541 atctcaacct tttgaagaag aataatttat tagctcacta aattgagggt aaggaaaagt
      601 aaaagcagta agaaaaatgt cttgcattat acagcaactt tttgggaatg agtagatgga
      661 ttgaataaaa tttgattaag agtggatgat ttatctgtag attattattg gacagttagt
      721 cttgaagtag tctaagaatt aggttataat cagtagaagc cttgctaata atgaggaggt
      781 tagtttat
//
LOCUS       DAVKLW010000130          781 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKLW010000130 DAVKLW010000000
VERSION     DAVKLW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.130"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>781
                     /gene="phtD"
                     /locus_tag="VW324_002103"
     CDS             <1..>781
                     /gene="phtD"
                     /locus_tag="VW324_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9727639.1"
                     /translation="HKLGAKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEA
                     LDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKY
                     TTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPS
                     TDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNI
                     KFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          254 a          154 c          169 g          204 t
ORIGIN      
        1 cataagctag gagctaagaa aactgacctc ccatctagtg atagagaatt ttacaataag
       61 acttatgact tactagcaag aattcaccaa gatttacttg ataataaagg tcgacaagtt
      121 gattttgagg ctttggataa cctgttggaa cgactcaagg atgtctcaag tgataaagtc
      181 aagttagtgg atgatattct tgccttctta gctccgattc gtcatccaga acgtttagga
      241 aaaccaaatg cgcaaattac ctacactgat gatgagattc aagtagccaa gttggcaggc
      301 aagtacacaa cagaagacgg ttatatcttt gatcctcgtg atataaccag tgatgagggg
      361 gatgcctatg taactccaca tatgacccat agccactgga ttaaaaaaga tagtttgtct
      421 gaagctgaga gagcggcagc ccaggcttat gctaaagaga aaggtttgac ccctccttcg
      481 acagaccatc aggattcagg aaatactgag gcaaaaggag cagaagctat ctacaaccgc
      541 gtgaaagcag ctaagaaggt gccacttgat cgtatgcctt acaatcttca atatactgta
      601 gaagtcaaaa acggtagttt aatcatacct cattatgacc attaccataa catcaaattt
      661 gagtggtttg acgaaggcct ttatgaggca cctaaggggt atactcttga ggatcttttg
      721 gcgactgtca agtactatgt cgaacatcca aacgaacgtc cgcattcaga taatggtttt
      781 g
//
LOCUS       DAVKLW010000131          671 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKLW010000131 DAVKLW010000000
VERSION     DAVKLW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.131"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..335
                     /locus_tag="VW324_002104"
     CDS             <1..335
                     /locus_tag="VW324_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9727640.1"
                     /translation="SIKHKKVTGVFNQMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLE
                     DAEKFATLFNSLNIDQVQLLPFHQFGENKYRLLNRKYEMDGVNALHPEDLIDYQKVFL
                     NHHINCYF"
     gene            410..607
                     /locus_tag="VW324_002105"
     CDS             410..607
                     /locus_tag="VW324_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9727641.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          242 a          115 c           88 g          226 t
ORIGIN      
        1 attctataaa acataaaaaa gtgactgggg tttttaatca aatgattatt aaaaacattc
       61 attatgcttt ttctcaaaat aaaactatcg ttttaagaat cccagttatt cctaatttta
      121 acaatagttt agaggatgca gaaaaattcg ctactctatt taactcatta aatatcgacc
      181 aagttcaact actccctttt catcaatttg gtgaaaacaa atatcgttta ttaaatcgga
      241 aatatgaaat ggatggagtc aacgcacttc atcctgaaga tcttattgat tatcaaaagg
      301 tatttctgaa ccaccatatt aattgttatt tctagttcat ttccttgaaa tgctctagct
      361 atttgcagat aacaagcatc tatataaggt tatcaaagaa gacttcccca tgactctaaa
      421 tgaatatatc ttacaatatc gcctgaaaca agctatagat aagatggctg aatctcccaa
      481 ctccccttta agcgctatct ctgaccaagt tggattttca gactataaat attttgccaa
      541 agtatttaaa aagtatctcc atatttcccc aaagaaattg aaatcactcg gaagaatagt
      601 aaaataggct attcttcttt ttgtagagaa ttttactcta atatagtgaa ttgaaataag
      661 atgtgaacat c
//
LOCUS       DAVKLW010000132          601 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKLW010000132 DAVKLW010000000
VERSION     DAVKLW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.132"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(154..>601)
                     /locus_tag="VW324_002106"
     CDS             complement(154..>601)
                     /locus_tag="VW324_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9727642.1"
                     /translation="RNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHPESDE
                     KENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEK
                     VTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESK"
BASE COUNT          138 a          127 c           95 g          241 t
ORIGIN      
        1 ttttagaagt agaggtatac tattctagtt tcaatctact atattttgca cattttcatg
       61 aaaaaaatga gaactagaac tcacattctg ctctcatttt tcgttttccc gttctcctat
      121 cctgttttta ggagttagaa aatgctgcta cctttactta ctctccttta ataaagccaa
      181 gagactatct acttctgctg aaatagtgtt attatctttc gttccgagaa gaagactact
      241 ttttagacca gtcaatgtct ccacagcatt ttgtctaata ctagaatctg ttactttttc
      301 tagcaaggcc tccgcatctg ctatcttagc gttaataaca gaatgctcta cttgaggaat
      361 ttcagcctca tctgtagtat cttcagcttc ttcctctgtc tcttccgtat cagtgcttgg
      421 tttataaaga ttatctgctg aaggatttaa accagcgtga ttctcttttt catcagattc
      481 agggtgagtt ggctcactta cttcatcatg ttccttatct tcctcaggtt tttctgtctg
      541 aggtttctcc tcgtttggtt tttccgtttg attggtatca gcttgaccat ttttgtttct
      601 t
//
LOCUS       DAVKLW010000133          578 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKLW010000133 DAVKLW010000000
VERSION     DAVKLW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.133"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>578
                     /locus_tag="VW324_002107"
     CDS             <1..>578
                     /locus_tag="VW324_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9727643.1"
                     /translation="NKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFL
                     DDDDEYMSDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQM
                     VHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAH
                     SKENGITGVTQKTINADEEYYNYCRKYFNLLS"
BASE COUNT          217 a           61 c          112 g          188 t
ORIGIN      
        1 gaaataaaat tatccatcaa tttggctata ccaatcatag aattaagtta attttaagta
       61 atgaagattt aggtgcaact aatgccagaa acataggtat caaaaattct agaggtaagt
      121 atatatcatt tttagacgat gatgatgaat atatgtcaga tcgaatttta aagttgatgg
      181 cttgttttaa aaagagtaga atgaagaatt tagctttagt ttatagttat ggaataataa
      241 tttatccaaa tggtacacga gaagaggaga agaccgattt tgttggaaat cccttgtttg
      301 ttcaaatggt tcacaatata gcaggtacgt cattttggtt gtgtaaaaaa gaggtgctag
      361 aattaattaa tggttttgag aaaatagatt cacatcagga cggtgttgtt ttattaaaac
      421 tacttgctca aggataccaa attgatatag tgcgagaatt cttggtgaat tactacgctc
      481 acagtaaaga aaacggtatc actggagtga cacaaaaaac aattaatgca gatgaagaat
      541 attataatta ctgtaggaaa tattttaatt tattgagt
//
LOCUS       DAVKLW010000134          567 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKLW010000134 DAVKLW010000000
VERSION     DAVKLW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.134"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..490)
                     /locus_tag="VW324_002108"
     CDS             complement(<1..490)
                     /locus_tag="VW324_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9727644.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KES"
BASE COUNT          196 a          101 c           64 g          206 t
ORIGIN      
        1 agctttcttt catggaaatt aagcgtttag caatcatatc aatacttctt gcatccactg
       61 ttgataaagt ctcatcaata aaaatataat cccaattttt agcaaaaatt aaggataagc
      121 ctaatttgaa ttttgttcca gtagatgctg tcatatagtt atctccgatt ttattatcaa
      181 aaaaagtatt aatttgttct ttatcctcaa atgatagatg gatttgaaaa gaatcacaca
      241 acaactgcaa attttcttct atagtaaatg gaacaacccc aagctgattg gtatcaaggt
      301 atagtacatc tttggagaca caattcttta aaccactatc aaaagtctct agcccagcta
      361 aaagttgtag aagggtgctc tttcctgaac cattctctcc ttcaactctg taaattccct
      421 tatcaatctc tagtgatata tttttcaata tacatttatc ataactttta gattcatttt
      481 caattttcat aatttaattc cctctatcaa cgctgctaaa attaataaaa gataagaaat
      541 gatataccat tttaaattac tttttat
//
LOCUS       DAVKLW010000135          499 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKLW010000135 DAVKLW010000000
VERSION     DAVKLW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.135"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..>499)
                     /locus_tag="VW324_002109"
     CDS             complement(<1..>499)
                     /locus_tag="VW324_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727645.1"
                     /translation="LGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDV
                     TISFYAKANKNGIMLRTRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWL
                     FNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRANSLEAGVNRLT
                     EGLRT"
BASE COUNT          129 a          110 c           88 g          172 t
ORIGIN      
        1 tttgttctga gaccttcagt cagacgattc acaccagctt cgagcgagtt ggctcgttgt
       61 ttgaaggtcg attctactgt tgaaatctga ccttctatat cttcaggagc ttctgaataa
      121 gaagtatcta catcgcttat ttcaaacttc ggcatccaaa tccaaacggt tccttcctgg
      181 ttgaaattga acaaccattc atttgtggtc tgcttggatt cgtttgtcca accttttgga
      241 atatggacaa catatcgctt aatttctgtc gacaatgtca catttccagt tttatatccg
      301 atattcccta atcgagttct tagcattatt ccatttttat ttgccttagc ataaaaacta
      361 atggttacat cttgattagt cgtacttccg ggaattactt tcccgaattg acccagagct
      421 ggataagtaa ccttgggatt acctccatca cggccagatg gattcaaacc tataatttta
      481 agagccttgt gtccaagat
//
LOCUS       DAVKLW010000136          428 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKLW010000136 DAVKLW010000000
VERSION     DAVKLW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.136"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>428
                     /locus_tag="VW324_002110"
     CDS             <1..>428
                     /locus_tag="VW324_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9727646.1"
                     /translation="TTRTKYCSQILQLLLKYFDKNPNKISEVYSILEANYGLVTEREY
                     IDYTLAENTISELANLDLTKSYNQELIVTILKQFLKIEIERTEAHEEKFTFGRYKVPD
                     SEKLKQYHRSILKLLANLYNIGSCETRFYIEKMLYDYRRK"
BASE COUNT          170 a           51 c           73 g          134 t
ORIGIN      
        1 aactacacgg acaaaatatt gtagtcaaat tctccaatta ttgttaaagt actttgataa
       61 aaatcctaat aaaatttctg aggtttatag tattcttgaa gcaaactacg ggttggttac
      121 agaaagagaa tatattgatt ataccttagc tgaaaatacc atttctgaac ttgctaatct
      181 tgatttgacg aaaagttata accaagaatt aatcgtgaca attttgaagc agtttcttaa
      241 aattgaaatc gaaagaacag aggctcatga agagaaattt acttttgggc gctataaagt
      301 cccggatagt gaaaaattaa aacagtatca tagaagtata ctaaaacttt tagcgaattt
      361 gtataatata gggagttgtg aaactagatt ttatattgag aaaatgctat atgattacag
      421 aagaaaaa
//
LOCUS       DAVKLW010000137          394 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKLW010000137 DAVKLW010000000
VERSION     DAVKLW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.137"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            complement(<1..351)
                     /locus_tag="VW324_002111"
     CDS             complement(<1..351)
                     /locus_tag="VW324_002111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9727647.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQY"
BASE COUNT          164 a           66 c           50 g          114 t
ORIGIN      
        1 atactgactc ataccttgat accaaataaa attcggtatt tcaataagag catgaggtaa
       61 tattcctttt attgtagcta ctattccaat catctgacca gatataccca attgtattcc
      121 taaagatagc aaatcaaaaa gttgcaataa gggcgaaatc ggaaaacaga atagatatag
      181 caaaaaattt gtagaatttg cctgtaaaac tgtcaatgta aattgtaatt ccagtttcgg
      241 aggtatagta gcaaaagtct cattttttaa tataaagctt aaaagtaaac ctagtaaaaa
      301 acagactata caaaataaca catagtaaat cagaaaacca ctttttctca tctaatgccc
      361 cctactaaaa ttacaaatat tatcagaaaa caat
//
LOCUS       DAVKLW010000138          367 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00053672
            SAMN33606439-rid17948433.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKLW010000138 DAVKLW010000000
VERSION     DAVKLW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606439
            Sequence Read Archive: SRR23727361
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722403.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722403.1
            Genome Coverage       :: 174x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:29:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,112
            CDSs (total)                      :: 2,065
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 64 of 131
            Pseudo Genes (incomplete)         :: 58 of 131
            Pseudo Genes (internal stop)      :: 36 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606439-rid17948433.denovo.138"
                     /strain="AUSMDU00053672"
                     /serotype="19A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST416"
     gene            <1..>367
                     /locus_tag="VW324_002112"
     CDS             <1..>367
                     /locus_tag="VW324_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9727648.1"
                     /translation="SRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWV
                     PDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVS
                     RYIPAKDLSAETAAGIDSKL"
BASE COUNT          115 a           91 c           72 g           89 t
ORIGIN      
        1 caagtcgaac cgccagaggt gtagctgtcc ctcatggtaa ccattaccac tttatccctt
       61 atgaacaaat gtctgaattg gaaaaacgaa ttgctcgtat tattcccctt cgttatcgtt
      121 caaaccattg ggtaccagat tcaagaccag aacaaccaag tccacaatcg actccggaac
      181 ctagtccaag tctgcaacct gcaccaaatc ctcaaccagc tccaagcaat ccaattgatg
      241 agaaattggt caaagaagct gttcgaaaag taggcgatgg ttatgtcttt gaggagaatg
      301 gagtttctcg ttatatccca gccaaggatc tttcagcaga aacagcagca ggcattgata
      361 gcaaact
//
